LOCUS       DATMED010000001         6299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4987960, whole genome shotgun sequence.
ACCESSION   DATMED010000001 DATMED010000000
VERSION     DATMED010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4987960"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VNP73_00005"
     CDS             <1..903
                     /locus_tag="VNP73_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL64333.1"
                     /translation="DAVIAVSPPLTLGLTGWAIARARRARFVFNLQDIFPDVAVELGA
                     LTDPRVIAAARRVERLCYSLSDAVTVLSDDLKANVAAKGVSENKIHIIPNFVDTEGIT
                     PAPKENRYRAEFGLEGKAVVMYAGNVGLSQSLGTVLDAAGALAYDDSIVFVINGQGAL
                     REELAAKARGMDNVVFVDMQPIDRVPDVLAAADIHLVPLRKGLAHSSVPSKTYSILAA
                     GRPFIAGVDAGTEIAHLADRSGAGIAIPPEDPEALTKALRSLIDDPETTAEMGASGRS
                     FIEGWASPEAVARSYEDLIGDLIR"
     gene            900..1874
                     /locus_tag="VNP73_00010"
     CDS             900..1874
                     /locus_tag="VNP73_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWL64334.1"
                     /translation="MIRRLALLLVCGIACSAGTGSPPSEARVDQGGLEWKRLAPVPTP
                     RTEVTAAAIGDRIYVIGGFADPGDTVPTVEIYDISTDDWSTGPDLPVPVNHSMAATLH
                     GDLYVAGGYLGPGVDNPTRTAFVLNGDRWMRIAPLPEPRGAGGLVAIDGRLYLVGGVE
                     GDDHLADETFVYHPRGDKWTKRRGLPGTRQHLGAAGARGRVFVVGGREHGLDTNTGRS
                     DRFNPRSRKWSRMASMPTPRGGLAATATLGFVVAAGGEADRTFDEVEAFDVERRRWRQ
                     LPDMPTARHGLGVVAVGSTVYVLAGGPEPGLTYSDANEALDLAPLRGS"
     gene            complement(1834..2481)
                     /locus_tag="VNP73_00015"
     CDS             complement(1834..2481)
                     /locus_tag="VNP73_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWL64335.1"
                     /translation="MTLQARVREVDDPIGMLVLIHGRGADENDLFPLFDILDPDGRLV
                     GVCPRGPLELPPGGAHWYVVREVGYPGPETFFPTFERASRWLDETVAQYGLGFDSVIL
                     GGFSQGCVMTYALAFAAGRPAPAGVLALSGFIPTVEGFELDLTKETRVAIGHGTYDPI
                     IEVGWGRDAHRRLSEAGADVTYTEYPLPHAIDPGYAQELQVWIKTLLEERGPGPR"
     gene            complement(2486..3907)
                     /locus_tag="VNP73_00020"
     CDS             complement(2486..3907)
                     /locus_tag="VNP73_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWL64336.1"
                     /translation="MWPFKRESDPFSSARYSGMFKSVRTRQRAHLRHWWQWALLGLFG
                     LIIVLGGFGIWRYFDLKCKISCGADPTAGAPEGEPFNVLLIGSDSRAGLTEQEQFDFG
                     AEEVGGERADTLILAHIDPANNHVTMVQFPRDLYVPIAGGGSNKINTALTGGVRGLVR
                     TVEQLTHLRIHDFAKVNIAGFRDLVDAIGGVELCMTEPLAFDPQTGIEITEEEVPGLV
                     HFDGERALRFVRSRNFPTGDFERIQNQQRFVSAAINKVTSSETLFHPTRILKLVDAAG
                     KHLRASLDPFQLKDLADRLKNFDPEHYEAYTAPNLGTAANEAGSVVLPDEPTMDLLFK
                     AISRNESPAEADGVPNVEPSTIRVGVYNGSFIDGAATTVADELQAATDIGDGPVNVVE
                     VANAGRLNHKGTTIVYAPEALQMAELLGAALPQAELLEGRTKRGIDVAVIVGQRGFET
                     RQIVQINPIPIPKPGDVPAACRK"
     gene            3938..5341
                     /gene="fumC"
                     /locus_tag="VNP73_00025"
     CDS             3938..5341
                     /gene="fumC"
                     /locus_tag="VNP73_00025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWL64337.1"
                     /translation="MSDYRLDKDSLGEVRVPAERLWGAQTQRSIENFPIGRDRFVWGR
                     RVIRALGLLKKSAALANGELGELPQEKVDLIVKAADEVISGELDAEFPLVVWQTGSGT
                     QSNMNVNEVIANRANQLAGRELGSKKPVHPNDDVNRSQSSNDTFPTAMHIAAVEEVRE
                     NLIPAAEVLRNTLASKGDQFTDVVMVGRTHLQDATPVRLSQVFSGWVAQIDDAIASIE
                     RALPGVCKLAIGGTAVGTGLNSQARFGDLVAEKVAEETGRPFVAAENKFAALSAHDDL
                     VDLSGSMRRLAVALFKVANDIRMYAMGPRAGIAELKIPENEPGSSIMPGKINPTQSEA
                     MTMVCVQVLGNDTAVAFAGTQGAFQLNVFKPVMLHNVLESASLLADASRAFNDHCAVG
                     IEPDREVIRNHLENSLMLVTALTPHIGYEKSAEIALKAFRDNKSLKEVAVELGYVEAN
                     DFDKWVRPEDMTKPTDG"
     gene            complement(5390..5734)
                     /locus_tag="VNP73_00030"
     CDS             complement(5390..5734)
                     /locus_tag="VNP73_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64338.1"
                     /translation="MCRASGEIEAHLLSGRLGACGIETHHVADRAAGEAWLHNGASPW
                     IPIAVWVRRHQLDDARIVLAEIAFDQPAHESRSTVSRWRAPMTWWTAAIALGLIFTGI
                     GFHRMAQYMDSL"
     gene            complement(5817..6299)
                     /locus_tag="VNP73_00035"
                     /pseudo
     CDS             complement(5817..6299)
                     /locus_tag="VNP73_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT         1219 a         1997 c         1971 g         1112 t
ORIGIN      
        1 gatgcagtca tcgcggtgtc accaccgctc acgctcggac taacggggtg ggcgatcgct
       61 cgtgcgcgta gggcacgttt cgtattcaac ctgcaagaca tctttcccga cgtcgccgta
      121 gagctgggag ccctcaccga cccgagggtc atcgccgcag ctcgcagagt ggaacgcctc
      181 tgctattccc tgagcgacgc ggtcacggtg ctgtccgacg atctcaaggc caacgttgcg
      241 gctaagggtg tgtcggagaa caagatccac atcatcccga acttcgtcga caccgaaggc
      301 atcacaccgg cccccaaaga gaatcggtac agggcagagt tcggactcga aggaaaggcc
      361 gtcgtcatgt acgcgggcaa cgtcggtctg tcgcagtcgc tcggcaccgt gctagacgcc
      421 gcgggagcac ttgcatacga cgactcgatc gtcttcgtga tcaacggaca aggtgccctg
      481 cgcgaagagt tggctgcgaa agcacgcggc atggacaacg tcgtgttcgt ggacatgcag
      541 ccgatcgatc gcgtaccgga tgtactggcg gcggcggaca tccatctcgt ccccctccgg
      601 aaaggtcttg cccattcgag tgtcccttct aagacctatt cgatcctcgc tgcgggccgg
      661 ccgttcatag ccggtgtcga tgccggaacc gagatcgcgc acctcgccga tcgctcgggc
      721 gcggggatcg cgatcccacc cgaagatccc gaagcgttga caaaggcatt gcgcagcctg
      781 atagacgacc ccgaaacaac cgcggagatg ggtgcgagcg gccgaagctt catcgaagga
      841 tgggcttcgc cggaggccgt cgctcgctcc tacgaagacc tgatcggcga cctcatccga
      901 tgatccgcag gctcgccctg ctcctcgtct gcgggatcgc ctgctctgca gggaccggct
      961 cgccgccgtc cgaggctcgg gtggaccagg gggggctgga gtggaaaagg ctcgcgccgg
     1021 ttccaacccc caggacggaa gtaacggcgg cagcgatcgg cgacaggatc tacgtcatcg
     1081 gaggcttcgc cgatcccggc gacacggttc ccaccgtcga gatctatgac atctcgacgg
     1141 acgattggag cacggggccg gacctgcccg tcccggtcaa ccactcgatg gctgcgaccc
     1201 tccatggaga cctctacgtc gccggcggat atctcggtcc gggcgttgac aatccgacgc
     1261 gaacggcttt cgtcctgaac ggcgatcgct ggatgcgaat cgccccactt cccgagccga
     1321 gaggcgcggg cggcctggtc gcgatcgatg gtcgcttgta cctggtggga ggagtagaag
     1381 gcgacgacca tctagcggac gagaccttcg tctaccaccc ccgcggagac aaatggacca
     1441 agcggcgcgg cctgcctggg accaggcagc acctcggtgc cgccggagca cggggacgcg
     1501 ttttcgtggt cggtgggcgc gaacacgggc tcgataccaa tacgggtcga tcggaccggt
     1561 tcaatccccg atcgcgcaag tggagcagga tggcgtccat gccgacccct cgcggtgggt
     1621 tggcagcaac cgcaaccctg gggttcgtcg tcgcggccgg gggtgaagcg gaccgcacgt
     1681 tcgatgaggt cgaagcgttc gatgtggaac gcaggagatg gaggcagctc cccgacatgc
     1741 cgacggcccg tcacgggctc ggggtcgttg cggtcggcag caccgtttac gtccttgccg
     1801 gaggccccga accgggcttg acctattcgg acgctaacga ggccctggac ctcgctcctc
     1861 taagagggtc ttgatccaca cctgaagctc ctgcgcgtag ccgggatcga tcgcgtgcgg
     1921 aagcgggtac tccgtgtagg taacatcggc accggcctcg gacaaacgcc tatgggcgtc
     1981 gcgcccccac ccaacctcga tgatcgggtc atacgtcccg tgaccgatgg cgacacgcgt
     2041 ctccttcgtc agatcgagtt cgaatccctc gaccgtcggg atgaacccgc ttaacgcgag
     2101 cacgccggcc ggcgccggcc gcccggccgc gaacgcaagc gcgtacgtca tcacgcaacc
     2161 ttgtgaaaac cctccgagga tgaccgagtc gaaaccgagg ccgtactgcg ccacggtctc
     2221 gtcgagccat ctcgacgctc gttcgaacgt cgggaagaag gtctcgggac cggggtatcc
     2281 cacctcacga acgacatacc agtgcgcgcc gccgggggga agctcaaggg ggccgcgtgg
     2341 acagactcca acgaggcgac cgtccgggtc gaggatgtcg aacagcggaa agagatcgtt
     2401 ctcgtccgcg ccgcgaccgt ggatcaggac gagcatcccg atcggatcgt cgacctcgcg
     2461 cacccgagcc tggagagtca tgtacctact tcctacacgc cgcgggcacg tctcccggct
     2521 tggggatcgg gatcgggttg atctgaacga tctgcctggt ctcgaagcct ctctggccga
     2581 cgatcactgc gacgtctata ccccgcttcg ttcggccctc tagcaactct gcctgcggaa
     2641 gagccgcgcc cagtagctcc gccatctgca acgcttcggg tgcgtagacg atcgtggtgc
     2701 ccttgtggtt cagccggccg gcattcgcaa cttccacgac gttcacgggc ccgtcgccga
     2761 tgtccgtggc cgcctgcagc tcgtcggcga ccgtcgttgc cgcaccgtcg atgaaggagc
     2821 cgttgtagac cccaacccgg atcgtgctgg gctcgacgtt ggggactccg tctgcttccg
     2881 ctggtgattc gttgcgagag atcgccttga agagcaagtc catcgtgggc tcgtcgggaa
     2941 gcacgacgct tcccgcctca ttcgcggccg tccccagatt tggggcggta taggcctcgt
     3001 agtgctccgg atcgaagttc ttcagccggt cggctagatc cttcaactgg aatggatcca
     3061 gactggcgcg cagatgcttc ccggcggcgt cgacgagctt cagtatgcga gtgggatgaa
     3121 aaagcgtctc cgacgaagtg accttgttga tcgcggcgga gacgaaacgc tgctggttct
     3181 ggatccgctc gaagtctccg gtcgggaaat tacgggacct cacgaaccgt agagcgcgct
     3241 ccccgtcgaa gtgcacgagc cccggaactt cttcctcggt tatctcgatc ccggtctggg
     3301 gatcgaacgc tagtggttct gtcatgcaca gctccacccc accgatcgcg tcgaccaaat
     3361 cgcggaagcc tgcgatgttg acctttgcga aatcgtggat ccgcagatgc gtaagctgct
     3421 cgaccgttct aaccaatccg cgcacgcccc ctgtaagagc cgtattgatc ttgttgctcc
     3481 caccccccgc aatgggaacg taaaggtcgc gagggaactg aaccatcgtg acgtgattgt
     3541 tcgcagggtc gatgtgggca aggatcaagg tgtcggcgcg ttcgccacct acttcctccg
     3601 caccgaaatc gaactgctcc tgctccgtga gcccggcacg cgaatcggag ccgatgagca
     3661 agacgttgaa gggctcaccc tcgggcgcgc cggcagtggg atctgccccg cacgagatct
     3721 tgcatttcag atcaaagtac cgccagatcc cgaaaccgcc cagcacgatg atcaacccga
     3781 acaatcccag caacgcccat tgccaccagt gccgcaggtg ggcacgctgg cgcgtccgca
     3841 cgctcttgaa catgccggag taacgcgccg atgagaaggg atctgactca cgcttaaaag
     3901 gccacatccc taccgattgt ccggcatgat tggaagcatg agtgactacc gcctcgacaa
     3961 ggacagcttg ggagaggtcc gggtcccggc cgagaggctg tggggagctc agacccagcg
     4021 ctcgatcgag aacttcccga taggccggga tcgcttcgtg tggggccgac gagtgatcag
     4081 agcactcggt ctgttgaaga aatctgcagc tctcgcgaac ggcgagctgg gcgagcttcc
     4141 ccaggagaag gtcgacttga tcgtgaaggc ggccgacgag gtgatctcgg gcgagctgga
     4201 cgccgagttc ccgctcgtcg tctggcagac gggctcggga acccaatcga acatgaacgt
     4261 gaacgaagtg atagccaacc gagccaacca actggccggt cgcgagctcg gctccaagaa
     4321 acccgtccac ccgaacgacg acgtcaaccg gagccagtcc tcgaacgaca ccttcccgac
     4381 ggcgatgcac atcgccgcgg tggaggaggt ccgagagaac ctcatacctg cggccgaggt
     4441 gttaaggaac accctggcct cgaagggcga tcagttcacc gatgtcgtca tggtcggcag
     4501 gacccatctg caggacgcca caccggttcg gttgtcgcaa gtgttctccg ggtgggttgc
     4561 tcagatcgac gacgcgatcg cctcgataga gcgcgctctt cccggcgtct gcaaactggc
     4621 catcggtgga accgcagtag gtacgggact caactcacaa gcgcgtttcg gcgacctcgt
     4681 cgcagagaaa gtcgcggagg aaacgggtcg gccattcgtc gcggcagaga acaagttcgc
     4741 cgcgctgtcg gcccacgacg acctggtcga cctcagcggt tccatgagga ggctggccgt
     4801 cgctcttttc aaagtggcga acgacatccg tatgtatgcg atggggcctc gagccggaat
     4861 cgcagagttg aagatcccgg agaacgagcc cggttcgtcg atcatgccgg gcaagatcaa
     4921 tccgacacag tccgaagcta tgacgatggt ctgcgtgcag gttctcggca acgatacggc
     4981 ggttgctttc gcaggaactc agggagcatt ccagctgaac gtgttcaagc cggtgatgct
     5041 ccacaacgtc ctcgagtctg cgagcctgct cgccgacgca tctagagctt tcaacgacca
     5101 ttgcgcggtt ggtatcgagc ccgatcgtga ggtcatccgg aaccacctag agaactcgct
     5161 gatgctggtc accgccttga ccccccacat cggctacgag aagtcggcgg agatagccct
     5221 caaggccttc cgggacaaca aatccttgaa ggaagtggcc gtcgagctcg gctacgtcga
     5281 ggcgaacgac ttcgacaagt gggtccgtcc cgaagacatg accaagccga cggatggtta
     5341 gttgggcagt gcgcgggcgg tactaactgg gcgggcgtag cccgctccgc taaagcgagt
     5401 ccatgtactg ggccatccgg tggaacccga taccggtgaa gatgagtcca agggcgatcg
     5461 cggccgtcca ccacgtcatg ggggccctcc acctcgagac ggtcgagcga ctttcgtggg
     5521 cgggctgatc gaaggcgatc tcggcgagaa cgatccgggc atcgtcgagt tgatgacgac
     5581 gaacccagac ggcgataggg atccacggac tggccccgtt gtggagccag gcttcgccgg
     5641 cggcacggtc ggcgacgtga tgggtttcga tcccgcaagc tcccaaccgg ccgctcagta
     5701 gatgtgcctc gatctcgcca gatgctcggc acaatggaac ccatcccccg ccactaccgc
     5761 cgtccaaccg gacccgtggg ggtggaggca taacggcagt ggatcccatc gcgcgcctag
     5821 cccttgccga agatgctgcg cgcgataacc acgcgctgga tctgattcgt tccttcgtag
     5881 atctgagtga tcttggcgtc acgcatgaat cgctcgaccg gatactcctt gacgtagccg
     5941 tacccgccca gcgcttggac gcaatcggtc gtcaccgaca tcgctacgtc cgacgcatag
     6001 cacttcgcga tcgcggccca gtaggaaacg tctgcatccg cgtcgtcgat cgcggtcgcg
     6061 gccttgtaca cggccatacg cgcggtctcc accttcatgg ccatgtcggc gaacatgaac
     6121 tgcagtcctt ggaaggcggc gatcggtttc ccgaactgct cacgctcctg aacgtacgag
     6181 catgcgaagt cgaacgcacc ctgggcgatg cccagagctt gggcggcgat cgtcggcgtg
     6241 agaaatccag cgtgcgcatc gcgtacttga aaccctcgtt gacctcgccg atcacgttc
//
LOCUS       DATMED010000002         3724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3837177, whole genome shotgun sequence.
ACCESSION   DATMED010000002 DATMED010000000
VERSION     DATMED010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837177"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="glgP"
                     /locus_tag="VNP73_00040"
     CDS             <1..906
                     /gene="glgP"
                     /locus_tag="VNP73_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWL64339.1"
                     /translation="KEMIQKLFHFAADPEVRLRFAFIEDYDMEVARFLCQGADIWLNT
                     PRRPLEACGTSGMKAALNGAVNCSILDGWWDECYNGANGWAIGTVYTYLDHEHQDRVE
                     SSALYDLLERDVVARFYDRPEGPIPRRWIERMKSSIESLGPFVTADRMLRDYVENLYE
                     PASRQSKVMNMEDHKRARDLAAWKARVRENWDGVEFLEVHGEETTADVGEPRMVDALL
                     RVGHLTTDDITVQLCHGRVGANGEILEPTIIEMKAGECDDQGLCSYQGSFATEAPGLY
                     GLAVRAVPAHRDLSSPMDMGLLVWA"
     gene            913..1272
                     /locus_tag="VNP73_00045"
     CDS             913..1272
                     /locus_tag="VNP73_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL64340.1"
                     /translation="MTPRILVIEDDDDLRSMMELAMEVSSIDLVGSAATANEGIELAI
                     EHQPDLVIMDVYLPDMDGIQATKVIMEKVPSTKVVGFTGRDARELNSMIEAGATAVYE
                     KTRFIELLQLVQAGEFD"
     gene            complement(1278..1631)
                     /locus_tag="VNP73_00050"
     CDS             complement(1278..1631)
                     /locus_tag="VNP73_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWL64341.1"
                     /translation="MSFEELVAEAIDSLPDEIAVHMQNVDVVVEDEPPLEYVRTLPRG
                     HTLLGHYHGIPLTRRGVHYDGALPDKISIYKGPITRMARTPNGIREQVRRTVIHEIAH
                     HFGIDDDRLHELGWG"
     gene            complement(1610..3334)
                     /gene="treZ"
                     /locus_tag="VNP73_00055"
     CDS             complement(1610..3334)
                     /gene="treZ"
                     /locus_tag="VNP73_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWL64342.1"
                     /translation="MWAPHARSVHLLLGDERVPLAKGVGDWWYADRPLEPGTDYLFSV
                     DDGTPRPDPRSPFQPSGPHGPSRTVDHSAFDWTDTNWCAPELRDAVIYECHIGTFTQR
                     GTFEAAIETLPHLVDLGVTALELMPVAEFPGSRGWGYDGVDLFAPHHAYGGPDGLKRL
                     VDAAHGHGLAMILDCVYNHLGPDGNHLAEFGPYFTDRYSTPWGQAVNLDDAYSTQVRS
                     FFIENVLMWMRDYHFDGVRLDAVHAIYDMSALHFLEELKASVASLEIELDRRLWVIAE
                     SDLNDPRLVADRSTGGYGLDAQWSDDFHHALHAILTGDRSGYYADFWSVAQLAKALQH
                     AFVYDGVYSRYRKRVHGRRPEGIPGYRFLGYMQNHDQVGNRATGERSSALMDPELLKV
                     AAALVLTAPFVPMLFMGEEWGASTPFQYFTDHNDELGRLVSEGRKREFASFGWEEAAI
                     PDPQDPATFERSRLDWGEPEDPKHADLLAWHRSLIRLRREHPDLTDGRLDAVRVDHDE
                     TARWLSLRRGGITVICNLSLDKIEVPLNADGLGRLRLTSSDPVVGPAGITLAPTSVSV
                     FECLSKSS"
     gene            complement(3342..>3724)
                     /locus_tag="VNP73_00060"
     CDS             complement(3342..>3724)
                     /locus_tag="VNP73_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64343.1"
                     /translation="AITNNTGTRYSGTFASEASVMPLNLGRETQEFSVEVAPSGWRVT
                     QEEAEVSLEVEVDKEPSLTHEPIATASATLEGLKAMAQGVCVTMSWDLELDAGDTFLV
                     ETRWRLRVGDVALTKRSRGVSAAL"
BASE COUNT          698 a         1133 c         1210 g          683 t
ORIGIN      
        1 aaggagatga tccagaagct gttccatttc gccgccgacc ccgaggttcg tctgcgattc
       61 gcattcatcg aggactacga catggaggtt gctcgtttcc tgtgtcaggg ggcagacatc
      121 tggttgaaca cgccgagacg ccccctcgaa gcttgcggga cgagcgggat gaaggccgca
      181 ctcaacggcg ccgtgaattg ctcgatcctc gatggatggt gggacgagtg ctacaacgga
      241 gccaacggct gggcgatcgg gaccgtctac acctatctgg atcacgaaca ccaagaccgg
      301 gttgagagca gcgctctata cgacctgctc gaacgcgatg tcgtcgctcg tttctacgac
      361 agacccgagg ggccgatccc gcgccgctgg atcgagcgga tgaaatcttc gatcgagagc
      421 ctcggcccct tcgtgaccgc cgatcggatg ctgcgcgatt acgtcgagaa tctctatgag
      481 ccggcgtcgc gacagagcaa ggtcatgaac atggaggatc acaaacgggc gcgcgatctc
      541 gcggcgtgga aggcgcgcgt gcgcgagaac tgggacggcg tcgagttcct cgaggtccat
      601 ggtgaggaaa cgacggccga cgtcggtgaa ccccgcatgg tcgacgcact tctcagggtt
      661 ggacatctga ccacggacga catcacggtt cagctatgcc atgggagagt cggggccaat
      721 ggagagatcc tcgagccgac gatcatcgag atgaaagccg gagagtgcga cgaccagggc
      781 ctgtgttctt accaggggtc gtttgcgact gaggccccgg gcctgtacgg cctcgccgtg
      841 cgtgccgtcc cggcccatcg agacctgtcc agtcccatgg acatgggtct cctcgtgtgg
      901 gcctagaggc gcatgactcc caggatcctc gttatcgaag acgacgacga tctccgctcg
      961 atgatggagt tggcgatgga ggtgtcgtcg atcgacctcg tgggttcggc cgcgacggcg
     1021 aacgaaggga tcgagctcgc gatcgagcac cagcccgatc tcgtgatcat ggacgtctac
     1081 ctccccgaca tggacggcat ccaggcaacg aaagtgatca tggagaaggt tccgtcaacg
     1141 aaggtcgtgg gtttcaccgg tagggacgcg cgcgaactca attcgatgat cgaagccggc
     1201 gcgacggccg tatatgagaa gacccgcttc atcgagttgc ttcaactggt ccaagcaggg
     1261 gagttcgact gagcgggcta accccagccc agttcgtgca agcggtcgtc gtcgatcccg
     1321 aagtgatgcg cgatctcgtg gatcaccgtg cggcgaacct gctccctgat gccgttgggc
     1381 gttcgcgcca tcctggtgat cggccccttg tagatcgaga tcttgtcggg cagtgctccg
     1441 tcgtagtgca cgccccgcct ggtcagcggg atgccgtgat agtggccgag cagcgtgtgc
     1501 ccgcgcggaa gggtccgcac gtattcgagc ggaggttcgt cttccaccac gacatcgacg
     1561 ttctgcatgt gaaccgcgat ctcgtcgggt aacgaatcga tagcctcggc tacgagctct
     1621 tcgaaagaca ctcaaatacc gaaacgctcg taggggccag ggttattccc gccggaccga
     1681 ccactgggtc cgatgaagtc aatcgcagac ggccgaggcc gtctgcgttc agtgggactt
     1741 cgatcttgtc caagctgaga ttgcagatga ccgtgatgcc acctctccga agggacaacc
     1801 agcgcgcggt ctcgtcgtgg tcgacgcgca cggcgtccaa ccgaccgtcc gttaggtccg
     1861 ggtgctcgcg acgcagtcgg atcaacgacc ggtgccacgc gaggaggtcc gcgtgcttgg
     1921 gatcctcggg ctcgccccag tcgagccggg accgctcgaa cgtcgcaggg tcttgaggat
     1981 cggggatggc ggcttcctcc catccgaacg atgcgaactc gcgcttacgt ccttcactaa
     2041 cgagtctgcc gagttcgtcg ttatggtccg tgaagtactg gaacggggtt gaggctcccc
     2101 actcttcgcc catgaacaac atcgggacga acggtgccgt tagcaccagg gccgcagcga
     2161 ccttgaggag ttcggggtcc atcagggcac tggatctctc gccggtggcg cggttgccga
     2221 cctgatcgtg gttctgcatg tatccgagga atcgataacc ggggatcccc tcgggcctcc
     2281 ggccgtggac ccgcttccgg tagcgagagt agacgccgtc atagacgaat gcatgctgca
     2341 acgctttcgc cagctgagcg accgaccaga aatctgcgta gtagccggat cggtctccgg
     2401 tcaggatcgc gtggagggca tgatggaagt cgtcgctcca ctgcgcatcc aagccgtaac
     2461 cgccggtgct ccgatcggcg acgagacgtg ggtcgttgag gtcgctctcg gctatcaccc
     2521 acaggcgccg atcgagttcg atctcgagcg atgcgaccga tgccttcaac tcttcgagga
     2581 aatgcaaggc cgacatgtcg tagatcgcat gaaccgcgtc gagccggact ccgtcgaagt
     2641 ggtagtcgcg catccacatc aagacgtttt cgatgaagaa gctccgcacc tgcgtgctgt
     2701 atgcgtcgtc aaggttcacc gcctgccccc agggtgtgga gtagcgatcg gtgaaatagg
     2761 gaccgaactc cgcgaggtga ttgccatccg gcccgaggtg gttgtagacg cagtccagga
     2821 tcatcgcgag cccgtggccg tgcgccgcgt ccaccaatcg tttcagtcca tcggggcctc
     2881 cgtatgcatg atggggcgcg aacaggtcaa ccccgtcgta tccccacccc ctggagcccg
     2941 ggaactcggc gaccggcatg agctcgagtg ccgtcacccc gagatcgaca aggtgaggga
     3001 gtgtctcgat cgcggcctcg aaggttccgc gctgcgtgaa ggtcccgatg tggcactcgt
     3061 agatcacggc gtcgcgaagt tcgggtgcac accaattcgt gtccgtccag tcgaacgccg
     3121 agtgatcgac ggtgcgcgag gggccgtgtg gtccgctcgg ttggaaaggg gaccgcgggt
     3181 cggggcgcgg ggtgccgtcg tcgacgctga agaggtaatc ggtgcccggc tccagaggcc
     3241 ggtctgcgta ccaccaatcg ccgacaccct tcgccagggg gacgcgttcg tcacccagca
     3301 ggagatggac ggagcgggca tgcggcgccc acaccagggc ctcagagtgc agcggagacg
     3361 cccctcgacc gcttcgtcag cgcgacatcg cctacacgga gccgccagcg cgtctcgacg
     3421 aggaacgtgt caccagcatc gagttcgagg tcccagctca tggtgacaca gacgccctgg
     3481 gccatggctt tcaacccctc gagcgtggcc gaagcggtcg cgatcggctc atgggtcagg
     3541 gacggttcct tgtcgacctc gacctcgaga gagacctcgg cttcctcctg ggtgacgcgc
     3601 catcccgaag gggccacctc gaccgagaac tcctgcgttt cccggcccag gttgagtggc
     3661 atcacggacg cttccgacgc gaacgttccc gagtagcggg tccccgtgtt gttagtgatc
     3721 gcgt
//
LOCUS       DATMED010000003         3967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1536617, whole genome shotgun sequence.
ACCESSION   DATMED010000003 DATMED010000000
VERSION     DATMED010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536617"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(967..1593)
                     /locus_tag="VNP73_00065"
     CDS             complement(967..1593)
                     /locus_tag="VNP73_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWL64344.1"
                     /translation="MSQLASVIGRAFRRGVLEDRTSSDVGPTGPGLLVFSPTGSLESL
                     NEDAAGWLTQLSREPDRRTRHGVLIPTEVYSVMSQARAVASGRSAAPARTRVRTLNGG
                     WLALHASCLRDGDGQIGDTAVVIEPAHASEIAPMIVQAYELTEREQTVTRMIAQGLST
                     AEIAEALVLSLHTVRGYVKAVFEKIGVASRGELVARLFADHYYGPLHR"
     gene            2268..3086
                     /locus_tag="VNP73_00070"
     CDS             2268..3086
                     /locus_tag="VNP73_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWL64345.1"
                     /translation="MSEWNFMDPSSRANLTKAWQREAEGMFELAAKPDRWEAPTGAGE
                     WQVRDVIGHLIDTTETYFISFDGARGKGEGPANLGLPDMARHVDEGARTHRGLPQKEA
                     LARLRTALDRMLGVAKELDDSEWAGLLVPHKYMGQLPAAFYPLFQLVDYGVHSWDIRE
                     GTGKGHLLDGDTADLLVPLAFIVWMSTPAVPPDTEPYTIGIQVNGGHNAGGRKVSVSS
                     AGVGVEEGAVDGLETVIEYDAATLVLSSYGRLNGGTVRGDRDLAERFLNSFFRI"
     gene            3091..3600
                     /locus_tag="VNP73_00075"
     CDS             3091..3600
                     /locus_tag="VNP73_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL64346.1"
                     /translation="MADVDKERIAAERKRISDKYLVEESKRPGPSARGIHHVALLSSD
                     VEKTVQFYQGVLEFPFIEIFENRDYEGSNHFFFDLGHGNALAFFDFPGLDLGPYAEVL
                     GGLHHVAISVELERWKKLRANIEAAGIEYAIHSEVSIYFRDPDGARIELIADPLGEMY
                     GTRLLSWQG"
     gene            3684..>3967
                     /locus_tag="VNP73_00080"
     CDS             3684..>3967
                     /locus_tag="VNP73_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64347.1"
                     /translation="MSTPRGSLEPIDEPIRAELFSVERLEQHARSMAEAHDLSGRPGR
                     LRLIARRLADNGEVLLSSYRELSKAIREEALLTPAAEWLVDNFHLVESQLR"
BASE COUNT          796 a         1204 c         1208 g          759 t
ORIGIN      
        1 ccggaccgcg cgtcgtcgtc gtcgccggaa aagtggtggt ggtcgttgga ttctgacgcg
       61 tcgtcgtcgt caccggtgga gcatttgtac tggtggtggt gttcgacggt cgagttgtag
      121 tagtcggagg tcgcgtgtgc cgactgctga gaaccggcgg gatattcgca atccccgcga
      181 cggttgtttc tgcctcgaca gcttggccga aaacgcgcac aagcgcatct tgaccaacga
      241 ccggataatc agcgacgact gccgagtcgc tgttcgcagc gaacgccacg acgtcgtcgc
      301 tgcttgtagt ggcacccgaa acgggccccg tattcaactt gcctattgcc aggagtgaca
      361 gcaccaaagc aacaagcaag acacctgcgg aggcgattct ttgcacgatc ttcccttcct
      421 tggacgaatt tgtatcggca agtccgaatc ggttatgaac cctggaagtc gcgactttgt
      481 cgctggacta gtcagtgggg ctcttgcttg attccggtcg gctggttcaa gttttcgtgt
      541 gtaggggagc gccgacgggc gctacctaag cagcatcaat tcgctcacgc acttctaaca
      601 aaggagaaat cgcgtgtcgt gcttgtttgt atttcgctct cttcgactgc gcctttgcgt
      661 tctctcaaca acaagtgaac gaacatgaag ttgcgaaaaa gagatgaaga tgacctcaat
      721 agaagttccg cgagggcagc gctgaaaact cgacgatgtg atcacaacaa caaccggttt
      781 ctggacgtcg cctctgatcg aaaacgacga tccctcgcaa aggccttatg accattgatg
      841 aaacccggta actagtgatc cggatgcgac agagtcaatt gacttgacgg aagaacttca
      901 gcttcgagaa ttggttgcgg catcaatcaa gcccgcgatg tcgactgaga ccaatcgatg
      961 agcgccctaa cgatgaagcg gtccgtagta gtgatctgca aacaagcgcg cgactagctc
     1021 accgcgactg gcaacgccga tcttctcgaa tacggctttg acgtaacccc gcactgtgtg
     1081 gagcgagagc accagcgcct cggcaatctc cgccgtgctc aatccctgag cgatcatccg
     1141 cgtgacagtc tgctcgcgtt cagtcaactc ataggcctgc acgatcatcg gcgcgatttc
     1201 ggacgcatgc gctggttcga ttacgaccgc cgtgtcccca atctgcccat caccatcgcg
     1261 aaggcacgag gcgtgcagcg ccaaccatcc accgttgagc gttctcactc gtgtgcgagc
     1321 cggggccgca gatcgtccgg aggcgaccgc gcgtgcttgt gacatcacgg agtagacctc
     1381 ggtgggaatc agcactccat gacgcgtgcg tcgatcaggc tcgcgagaca attgggtcaa
     1441 ccagccggcc gcgtcttcgt tgagtgattc caaacttccg gtcgggctga acaccaacag
     1501 ccccggaccc gtgggaccga catctgaaga agtgcggtcc tccaggacgc cacgccgaaa
     1561 ggccctaccg atgacggaag caagctgtga cattgttgtc acctcagtct cggtgaagtg
     1621 aggacgacct cgctgtcgcc acaggctggc gacgccccaa caacctgacc gtccccggaa
     1681 ggcgactcgc aactcgtcgt cgaacccgtt gggttgattc aactcccgat ggcgggtgct
     1741 gcgacccgga tggccctcgg ttgcggcctg catcgacccg gccggtacct ggcgccgagc
     1801 aggtctcgat acaggagaac gtcctcgatg aggagctctc gttcccagaa cgtgtggcac
     1861 tcctcgtcga cgctatcgag gcggactggc ggagccggca gggtggtagt cgggtcggtt
     1921 gcaacaaaca aggatgcgtc gaacggcacc agtcgccgaa gtcttctgtc gagttcctcg
     1981 aggaactcgc cgtcggccga acggtcccga cacaagtcca gtagatcttc acgccgccgg
     2041 tccgattccg gcgcatgcac gggcgccgcc accattggcg cagtatggcc ctcgaatcca
     2101 gcgggtgcca ccgctttcgg cacccctcat ttgagggggg tcgtccggca aagaacgccc
     2161 acatgtgggg ttgaccgtgc cctcaacgac agacatcgtt cttcagagcg cgtctccgcg
     2221 ctcagtaaag tccgcggacg gttgtaggag cctgaggagg aacggcgatg agtgaatgga
     2281 acttcatgga cccgagctcg agggccaacc tgacgaaggc ctggcagcgc gaagcagaag
     2341 gaatgttcga gttggcggcc aagccggatc gctgggaggc accaaccgga gccggcgaat
     2401 ggcaagtgcg cgacgtgatc ggccacttga tcgacaccac cgagacctac ttcatctcat
     2461 ttgacggcgc ccgggggaag ggcgaaggtc cggccaatct tggactccct gacatggctc
     2521 gccacgtcga cgaaggtgcc cgcactcatc gtggtctccc ccagaaggaa gcactcgccc
     2581 ggttgcgcac agctctcgat cgcatgctcg gtgtcgccaa ggaactagac gacagcgaat
     2641 gggccggcct gttggtgcca cacaaataca tgggccaact cccggcggcg ttctacccgc
     2701 tctttcaatt ggtcgactac ggggtccaca gctgggatat cagagaaggg accggcaagg
     2761 gccacctact cgacggcgat accgccgacc tgctggtccc gctggccttc atcgtgtgga
     2821 tgtcgacgcc cgccgttccg cccgacaccg agccgtacac catcggaatc caggtgaacg
     2881 gtggacacaa tgccggcggc cgcaaggtct cggtcagttc ggccggcgta ggcgtggaag
     2941 agggagcggt cgacggtctc gagacggtga tcgaatacga cgccgccacc ttggtcctct
     3001 cttcctatgg ccgcctcaac ggaggaacgg tgcgtggcga ccgagacctt gccgagcgtt
     3061 tcctcaactc gttcttccgg atctgagcac atggccgacg ttgataagga gcggatcgcc
     3121 gcggagcgga aacggatctc cgacaagtat ctagttgagg agagcaagcg gccgggtcct
     3181 tccgctcgtg ggatccacca tgtggccttg ctgtcctcgg acgtcgagaa gacagtgcag
     3241 ttctatcagg gagtcctcga attcccgttc atcgagatct tcgagaatcg agactacgaa
     3301 ggctccaatc acttcttctt cgatctgggc cacggcaacg cgctggcctt cttcgacttc
     3361 cccggtctcg acctcggtcc ctatgccgag gtcctcggcg gcctgcatca tgtggcgatc
     3421 tcggttgaac tggagcggtg gaagaagctc cgggccaaca tcgaggcagc tgggatcgag
     3481 tacgccatcc acagcgaagt gtccatttat ttccgcgacc ccgacggggc tcggatcgag
     3541 ttgatcgcgg atcccctcgg cgagatgtac gggaccaggt tgttgagctg gcagggctga
     3601 agccccgatt ccctccgttc tcgcggatca accgcgccct atagggcgcg gttgatgcgc
     3661 gagaaggcat gtagcgtgcg gtggtgtcga cgccccgagg ctccctcgag cccatcgacg
     3721 agcccatccg ggcggagctg ttcagcgtcg aacggcttga acagcacgcc cgcagcatgg
     3781 ccgaggctca tgacctgagc gggcggcccg gccggctgcg gctgatcgcc cggcggctag
     3841 cggacaacgg cgaagtcctt ctttcgtcgt atcgggaact gtcgaaggca attcgcgagg
     3901 aggccctgct cactccggcc gccgaatggc tggtcgacaa cttccatctg gtcgaaagcc
     3961 agctccg
//
LOCUS       DATMED010000004         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3843549, whole genome shotgun sequence.
ACCESSION   DATMED010000004 DATMED010000000
VERSION     DATMED010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3843549"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..386)
                     /locus_tag="VNP73_00085"
     CDS             complement(159..386)
                     /locus_tag="VNP73_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64348.1"
                     /translation="MNDALLVVAMAAVWWVPTFVCLSDLQRKSEASSERPPDERIRRV
                     VLWRWTAVLCVPVVGALLYWSRGRRELDGGA"
     gene            complement(397..1602)
                     /locus_tag="VNP73_00090"
     CDS             complement(397..1602)
                     /locus_tag="VNP73_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HWL64349.1"
                     /translation="MFATLQRVGRSLMMPIAVLPAAALALGLGFYFQTVSWLSWGDTI
                     WEIMTAGGNAVIANLPLIFAIGIAIGFTGGAGAAGLAAGVGYVILKAINDISIITEAS
                     EGVEEVVVDMGVLGGILIGLIAAVLYDRYKDIRLPDYLAFFGGRRFVPIVTAFAAIVL
                     GAVAYFVWPPIGRGINDFGDWIVGAGALGAFAYGIANRALLPMGLHHILNALVWFQFG
                     TYTPPGGDPVSGDLSRYLAGDPEAGLFMAGWFFIMMFGLPAAAYAMYRAAEPDQKKAT
                     GAVMGSTGFTSFLTGITEPIEFSFLFLAPLLYAAHAVLSGIALVVADGLNIKMGFGFS
                     AGLIDYLVNLHLATNAFLAIPVGLVFGVVYFVMFSFAIKAFDLPTPGRESVPEIGTEP
                     TTASGTPPA"
     gene            1831..>2587
                     /locus_tag="VNP73_00095"
     CDS             1831..>2587
                     /locus_tag="VNP73_00095"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HWL64350.1"
                     /translation="MNERDSNLDRLGTETEPNGLVGLDLMSTTELVELMNREDALVPG
                     AVALARDQVALAIDGIVDRLRGPGGLIYVGAGSSGRLAMLDASECGPTFDVGPERIQT
                     VLAGGETALVNASEEVEDDAEAGAADLSARSVSVHDAVVGISASGRTPYVLGALEHAH
                     GKKALTLGLVCNPSSPIARQVDLPIEIVTGPEVIGGSTRLKAGTAQKLVLNMISTITM
                     VRLGRTFGNLMVGVRGSNEKQRFRARRIIRLASG"
BASE COUNT          544 a          803 c          793 g          447 t
ORIGIN      
        1 aaccctcgag aatctcctga agctgctcgc ccttggggcg cccgaatgtt agctccttgg
       61 gaagatggag cgccggctcg atcgcggggt cactatcgga catgaggaaa ccctaatgag
      121 tgagcgcggc tagatccggg attgaagctc ctcggaaatc aggcgccacc atcgagttct
      181 ctccttcccc tgctccagta caagagcgcg ccgaccaccg gcacgcacag aaccgccgtc
      241 catctccaca gtacgactcg ccgaattcgt tcgtcgggtg gtctctctga gcttgcctcg
      301 ctctttcgct ggaggtcgct caagcacacg aaagtcggca cccaccacac cgccgccatc
      361 gcgaccacca gcaaagcgtc gttcaagatg tattgactac gccggcgggg ttccggacgc
      421 cgtcgtgggt tctgtcccga tctccggtac gctttctcgc cccggcgtcg gcaggtcgaa
      481 cgccttgatg gcaaacgaga acattacgaa atacacgacg ccgaacacca gacccaccgg
      541 tatcgcaaga aacgcattcg tcgcgaggtg caggttgacg aggtagtcga tgagtccggc
      601 cgagaagccg aagcccatct tgatgttgag cccgtctgcc acgaccagcg cgatccccga
      661 caggaccgcg tgtgccgcgt acagcaacgg agccaggaac aggaaactga actcgatcgg
      721 ctccgtgata ccggtgagga acgaggtgaa tccggtcgat cccatgaccg ccccggtcgc
      781 cttcttttgg tcgggctcgg ccgcgcggta catcgcgtag gcggcggccg gcaatccgaa
      841 catcatgatg aagaaccaac ccgccatgaa caacccggcc tccggatccc cggccagata
      901 ccgggagagg tcgccggaaa ccggatcgcc tccggggggc gtgtaggtgc cgaactggaa
      961 ccagacgagc gcgttcagga tgtggtgcag tcccatgggc agcaatgctc gattggcgat
     1021 tccgtaggcg aaggcgccga gagctccggc acccacgatc cagtcgccga agtcatttat
     1081 ccctcgtcct atcggaggcc agacgaaata ggcgaccgcg cccaggacga ttgccgcgaa
     1141 cgccgtgacg atgggcacga agcggcgacc tccgaagaac gccaggtagt cgggcagtcg
     1201 aatgtccttg tagcggtcgt acaacaccgc ggcgatcaga ccgatcagga tgccaccgag
     1261 gacccccatg tcgacgacga cctcctcgac cccctcgctt gcctcggtga tgatcgagat
     1321 gtcgttgatc gccttcagga tcacgtagcc gacgcctgcc gccagccccg cggcccccgc
     1381 tcctcccgtg aatccgattg cgatcccgat ggcgaagatg agcggcaggt tcgcgattac
     1441 cgcgttcccg ccggcagtca tgatctccca gatggtgtcg ccccacgaca gccaggagac
     1501 ggtctggaaa tagaagccaa gacccaatgc aagcgctgcc gcaggcagca ccgcgatcgg
     1561 catcatcagg gacctaccga cccgttgcag ggttgcgaac atggactctc ctccccttaa
     1621 gaccaccgcg gccaagcgat acgaggaccg cgaaaccaca tgcatcctaa tattggtcta
     1681 gaccagatgg caaggacacc gttatgtcag gctatggtag gaactcaact ggtctatacc
     1741 tttagagtcg gccaaccact gcaagggatg ctgggtttca acctgctcac actctgaatc
     1801 gacccgtact ggacgttgga gccacaaaag atgaacgagc gagactcgaa tcttgatcgg
     1861 ctcgggaccg aaaccgagcc aaacggcctc gtcggcctcg acctcatgtc gacaaccgag
     1921 ctggtggagt tgatgaaccg agaggacgct cttgttcccg gcgccgtcgc tctggcacgc
     1981 gaccaagtcg cgttggcgat tgacggcata gtcgaccgtc tgaggggccc aggaggactc
     2041 atctatgtgg gggcgggtag ttccggccgc ctcgccatgc ttgacgcgtc ggagtgtggc
     2101 cccacctttg atgtcggtcc cgagcggatc caaacggtgc tggcgggagg agagacggcc
     2161 ttggtgaacg catcggagga agtcgaggac gacgcggagg ccggagccgc agacctaagc
     2221 gcaagatcag tgagcgttca cgatgcagtc gtgggcatca gcgcgagcgg gaggacaccc
     2281 tacgtgctgg gggctctcga gcacgcgcat ggtaaaaaag cgctgaccct cgggcttgtg
     2341 tgcaatccgt cctcgccgat cgcgcgacaa gtggacctac cgatcgagat cgtcaccggt
     2401 ccagaggtga ttggcggctc gacgcggctg aaggcgggca ctgctcagaa gcttgtcctt
     2461 aacatgattt cgacgatcac gatggtgcgt ctaggtcgca ctttcgggaa cctgatggtg
     2521 ggcgtgcgcg gttcgaatga gaagcaacgg ttccgcgcac gcagaattat tcggctggcg
     2581 tcggggt
//
LOCUS       DATMED010000005         6831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1158176, whole genome shotgun sequence.
ACCESSION   DATMED010000005 DATMED010000000
VERSION     DATMED010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158176"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..896)
                     /locus_tag="VNP73_00100"
     CDS             complement(186..896)
                     /locus_tag="VNP73_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HWL64351.1"
                     /translation="MPENDRLLQSLRELSSLLLSEETLRSILQRVASLSVTAIRACDA
                     AGVSVMDGAQVVTLASSDESVQVVDEIQYRLEQGPCLQAIHDGNIFRIDSIANEKRWP
                     AFVSEASKQGVRTCLAVPLIEDLSTFGALNLYSYRDEVFDNDDESAAVALAAQASGPL
                     SNMRHFEELGDIAASLTRASASPIDVAIGILMERNGWNRAAAINELRELASMDHGELV
                     PAARIVVESVVRRGDGGA"
     gene            complement(1034..1774)
                     /locus_tag="VNP73_00105"
     CDS             complement(1034..1774)
                     /locus_tag="VNP73_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HWL64352.1"
                     /translation="MEYPGDLTESFEALSTFLSTPEDVDATLSRIATLSVAVIPHCDF
                     AGVSLVEPGGIRTVGHTSELVKEIDGIQYETGEGPCLSAIKDPRASGHPATYEIPSMA
                     LDTEWPEFSAKARERGLASLLAFTLLSNERALGSLNLYATLPHAFAPLDRRIGAIFAA
                     HAAVALANAQTLESARRRVDELLEGYANREVIGQAKGILMEREGCTEEEAFELLRSAS
                     SRLHRKLREVAKGVIHSTSEGKRPPPPR"
     gene            complement(1822..2013)
                     /locus_tag="VNP73_00110"
     CDS             complement(1822..2013)
                     /locus_tag="VNP73_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64353.1"
                     /translation="MELNDVLRYAEEVALAIPRRFLESVNAGSIQNARREVIGADRRR
                     AIRSTLDQTNQDVDIHPGG"
     gene            complement(2004..2312)
                     /locus_tag="VNP73_00115"
     CDS             complement(2004..2312)
                     /locus_tag="VNP73_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64354.1"
                     /translation="MHISVAVDLLRALGLAMDAYDQERAGQPSNRETRMRARGVAERE
                     AIRRLMGTQSFLASLRVGAQTYVRERSYFRPLTFERAQEDSGEERAVLDLTVEEQAWN
                     "
     gene            complement(2457..4151)
                     /locus_tag="VNP73_00120"
     CDS             complement(2457..4151)
                     /locus_tag="VNP73_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HWL64355.1"
                     /translation="MIYLVLVAFIGGAVSALTPCVLPILPALLAVSGGQGRRRVIGIV
                     VGLELSFFLLAIVLAAILSSLGLSNSLLQWVAAALLVAFGLTLIVPSLNERFQSFVSR
                     VTGPLATAVPRKSGFAGGLLAGAPLGLIWAPCAGPILAGITVAGASMRFGGRTIVMMV
                     AYALGMLGPLLLIAIGGRRASDFLRKRFGSGRRVEFGMGIILILTAAMVGFGWANQIN
                     RFLAEWVNLTSTPTAALEEGALRRGGGLEAPEGTRVRLTDSQLVAAGYPELQELADYG
                     PAPDVKGISNWFNDEPLSIKELRGKVVLVDFWTYSCINCIRTFPYLKQWYADYRDDGL
                     VILGVHTPEFTFEKDPDNVSEAIDDFGIEYPVALDPNYETWNQYYNRYWPAHYLIDRD
                     GTLRSVHYGEGAYERTENEIRHLLSLPETAEVEEEEPSFTPRTPETYLGYARADRFAS
                     AGGLVPDEEARYEAPAELPTDWWAYSGTWTVTGQAAVAGPDASIALHFRAREVHMVAG
                     PTGSSSGTITVRTAGEERTIEADEHRLYTLRTVGYEDAVMDIEVSEGVEVYSFTFG"
     gene            complement(4205..5038)
                     /locus_tag="VNP73_00125"
     CDS             complement(4205..5038)
                     /locus_tag="VNP73_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWL64356.1"
                     /translation="MAVAAPGIAKRPVAAFLVMAYGVTVVVGLFPALTRRDLLPFDQA
                     PYDWLGHFLGSALPAFIVVWALSRRAGARDLARRCLRWRVPGRWYLVALLGVPVGTIL
                     LASLAYGSEPLSLIVQRWPLMFTTVLPHLLVIIVFSNVAEEVGWTGFLLDRMQDRFSP
                     MRAAVVTGAAFALAHLPGYVVEEGVAAAPILFGILVIPQIASRFIVAWIYNSTGRSIL
                     LVGLFHCAFNVTSADFSHEFIPGPRDEIFVFTSGLVVVLAAMIAIRTRGGLAFNDPSG
                     K"
     gene            5171..5398
                     /locus_tag="VNP73_00130"
     CDS             5171..5398
                     /locus_tag="VNP73_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64357.1"
                     /translation="MRKTRFAVLGVIVAVGSLTTFASPAHACNEVGDPACTVGGAVNA
                     SACVASLVAESAKTGEPINKHELADCFNQTN"
     gene            complement(5460..6191)
                     /locus_tag="VNP73_00135"
     CDS             complement(5460..6191)
                     /locus_tag="VNP73_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWL64358.1"
                     /translation="MGDLGDVYETTRSSVIELVREHATEQDRPVPATPEWRVRDVISH
                     LVGDVECILRGDFPREFFRSFGDADAIVDLNKWTQSQLEARDERSLDEIIAEWNELTP
                     DLVSILRGENEWPDGVTAFADTVIVTDLGVHQQDLFGAFGVERDRESPPVKIGSSAYM
                     AAVDLRIRMQEGDALGVEASGKRWVAGGDEPITTLRTDRFELFRALSGRRSPDQLRSY
                     EWDGDPEPFLEYFYPYGMREESLVE"
     gene            complement(6214..>6831)
                     /locus_tag="VNP73_00140"
     CDS             complement(6214..>6831)
                     /locus_tag="VNP73_00140"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWL64359.1"
                     /translation="LARGDEAMYERMAKLVEPSSHEAAILFCDLHGSTELARTLSTSR
                     YFRLIRSLWTEIDQLVAGSHGIVGKHAGDGASAFFLESDLGSPSATASAALRTARQIH
                     DRSQDVFGEALDTSCLMRIGLHWGSSLYIGQLVPGGRLDVTALGDAVNECARIQECAD
                     PHGTLASKDLIERLTANDAAALGIDPEKISYQLVPTLDGVPVAQL"
BASE COUNT         1351 a         2175 c         2058 g         1247 t
ORIGIN      
        1 ctgagcgcgt cgagcatggc ggccgaactg tcgttaacag gctctgggtt ctccatggcc
       61 acctcgcagc agccgcccca aagaaaaaac ccggcgtccc ttacatcttt cctcgcggga
      121 cggccaggtt gtaagccgag gagcgagcat actcccgctt ctcctgggct accaggaaac
      181 ttttctcagg cccctccgtc gccgcggcgc accacagatt cgaccacgat tcgggcggca
      241 ggcacgagtt cgccgtggtc catcgaggcc agctcccgca gctcgtttat agccgctgct
      301 cggttccatc cattgcgctc catgaggatc ccgatagcca cgtcgatggg tgacgcactc
      361 gctcgagtga ggcttgccgc gatgtccccc agctcctcga aatgacgcat gttcgacagc
      421 gggcccgatg cctgcgcggc caaggcgacc gccgcgctct cgtcatcgtt gtcgaagacc
      481 tcatcccggt acgaatagag gttgagggct ccaaaggtcg acaggtcctc gatcaacggc
      541 accgcgaggc atgtgcggac gccttgcttc gacgcttcac tcacgaaggc cggccaccgc
      601 ttctcgttgg cgatggaatc gatcctgaag atgtttccgt cgtggatggc ttgaaggcag
      661 ggcccttgct ccaagcggta ttggatctcg tccacgacct gcacgctctc gtcggagctt
      721 gcgagcgtaa caacctgagc accgtccatc acggagacac cggcggcatc gcacgcgcgg
      781 atcgccgtca cggaaagaga agcgacccgt tgcaggatcg atctaagcgt ttcctctgag
      841 agcagaaggg acgacagctc acgaagactt tggagcaaac gatcattttc gggcacgggg
      901 ccctcttcct tcgcaatggc gtcatcagcg cgctgctaag gaagggtcgg cttgtcgtcc
      961 ggtcccgggc aggtgaacgc tctgggcgtt ctcttcacta tacgtccccc aactcgatgg
     1021 atagacgcag gctctaccga gggggtggcg gtcgcttccc ctccgaggtc gagtggatga
     1081 cgcccttggc gacctcgcgc agcttcctgt gtaggcgtga ggatgcgctt ctcagcaact
     1141 cgaaggcctc ttcctccgtg caaccttctc gctccatcag gatgcctttc gcctgtccga
     1201 tgacctctcg attcgcatat ccctcgagca gctcatccac gcgccgacgg gcggactcca
     1261 atgtctgcgc gttcgccaga gcgactgcag catgggctgc gaagatcgct ccgatcctcc
     1321 ggtccagtgg agcgaacgcg tgcggaagcg ttgcgtacag gttgagcgat ccaagtgccc
     1381 gctcgttgga caacaacgtg aaggcgagca ggctcgcaag cccccgttcc cgtgctttcg
     1441 cagagaactc cggccactcg gtgtcgaggg ccatcgaagg gatctcatat gtggccgggt
     1501 gcccggacgc gcgcggatcc ttgatcgcgc ttaggcacgg cccctctccc gtctcgtatt
     1561 ggatcccgtc gatctctttc acaagttccg acgtgtggcc gacggttcgg ataccccccg
     1621 gctcgactaa agagaccccg gcgaaatcac agtgtgggat gacggccaca gagagtgttg
     1681 cgatgcggga cagagttgcg tcgacgtcct ccggcgtgga caggaaagtg gagagcgctt
     1741 cgaaactctc cgttagatct cctggatatt ccactgggca cctgaccttc gggatgcctg
     1801 gccgtctata ccaattctcc cctatcctcc cgggtgtatg tcaacgtctt ggttggtttg
     1861 gtcgagggtc gacctgatcg cccggcgtcg atctgccccg atgacttccc ggcgtgcatt
     1921 ctggatcgaa cccgcgttga cggactccaa gaaacgccgg gggatggcga gcgccacctc
     1981 ttcggcgtat ctcagcacgt cgttcagttc cacgcctgct cctccactgt cagatccaaa
     2041 acagcgcgct cctcgccgct gtcttcctgt gcccgttcga acgtgagggg gcggaagtag
     2101 ctcctctcgc gcacgtaagt ctgggctcca acccgaagcg aggcgaggaa tgactgggta
     2161 cccatgagcc gtcggatcgc ttccctttcc gcgacccctc gggcacgcat ccttgtctct
     2221 cgattcgatg gttgcccggc tcgctcctga tcgtacgcgt ccatcgcgag cccgagggct
     2281 ctgagcagat cgacggctac agagatgtgc atgaccttcc ccctcctagc tcctctgccg
     2341 cgccggccgg gaggggtggg cacaaaaaaa cccggccgct ccacaaaacg cggaaggcca
     2401 ggttgtaagc cggagtcaca ttactaactt catcctcgag ccgcaacgga tgccttctag
     2461 ccgaaggtga acgagtacac ctcgacccct tcggagacct cgatatccat gaccgcgtcc
     2521 tcgtatccca ccgttcggag tgtgtagagc cggtgctcgt cggcctcgat ggttcgctcc
     2581 tccccggctg tgcgaacggt gatggtcccg gaggaggaac cggtcgggcc ggcgaccata
     2641 tggacctccc gagccctgaa gtgcagcgcg atcgacgcat cggggccggc gactgcggcc
     2701 tggccggtga cggtccaggt acccgaatag gcccaccagt ccgtcggaag ttcggcgggg
     2761 gcctcgtaac gcgcctcctc gtcgggcaca agcccccccg ctgaggcgaa gcgatcggcc
     2821 ctggcatatc ccaggtaggt ctcgggcgtc cggggcgtga agctcggttc ctcttcctct
     2881 acttcggcgg tttccggaag agacagcaag tgtcggatct cgttctcggt tcgttcgtac
     2941 gctccctcgc cgtagtgcac ggagcggagc gtgccatcgc ggtcgatcag ataatgggcc
     3001 ggccagtacc ggttgtagta ctgattccag gtctcgtagt tgggatcgag ggcgaccggg
     3061 tactcgatgc cgaagtcatc gatcgcttcc gaaacattgt ccggatcctt ctcgaaagtg
     3121 aactcgggcg tgtgcacccc gaggatcacg agaccatcat cgcgatagtc cgcgtaccac
     3181 tgcttcagat agggaaaggt gcggatgcag ttgatgcagg agtaggtcca gaagtcgacg
     3241 aggaccacct tcccccgcaa ttccttgatc gacagtggct cgtcgttgaa ccagttactg
     3301 atccccttca cgtcgggggc cgggccgtaa tcggcaagct cttgcagctc tgggtatccc
     3361 gcggcgacga gctgagagtc cgtgagtctc acccgcgttc cctccggcgc ttcgagccct
     3421 ccaccgcgtc gcaatgcgcc ctcctccagt gctgccgtgg gtgtggaggt caggttcacc
     3481 cactctgcca agaagcgatt gatctgattg gcccagccga agccgaccat cgcagccgtg
     3541 aggatcagga tgatgcccat gccgaactca acgcgccggc cgctaccgaa tctcttgcga
     3601 aggaagtccg acgcccgacg cccgccgata gcgatgagca gaagggggcc gagcatcccc
     3661 agtgcgtatg ccaccatcat cacgatcgtg cggccgccga atcgcatgga tgctcctgcc
     3721 acggtgatgc cggcgaggat cggtcccgcg cagggggccc agataaggcc aagaggagcc
     3781 cccgccaata gacctccggc gaaaccgctc ttgcgaggga ccgcggttgc aagaggaccg
     3841 gttacccgag acacgaacga ctggaagcgt tcgttaagac tcggaacgat cagcgtcaag
     3901 ccaaaggcca cgaggagagc ggccgcgacc cattgcagga gcgagttgga caagccgagc
     3961 gaactaagga tggctgcaag cacgatcgcc agcaggaaga acgagagctc gagaccgacc
     4021 acgatcccaa tgacccgacg ccggccctgc ccccccgaaa ccgcaaggag ggccggcaag
     4081 atcggcagca cgcaaggggt cagcgcggat accgcgcccc cgatgaatgc gacaaggacc
     4141 agatagatca aagcgactcc ctgagaaaag gcgtctcgcc ctcaatcatg gaccggccga
     4201 tggcctactt cccactcgga tcgttaaaag ccaacccgcc cctggttcgg atcgcgatca
     4261 tggccgcaag aacaacgacc aatcctgagg taaatacgaa gatctcatcc cgggggcccg
     4321 gaatgaactc gtgggagaaa tcggcgctcg ttacgttaaa agcgcaatgg aagaggccga
     4381 ccaagaggat gctgcgcccg gtgctgttgt agatccacgc cacgatgaag cggctggcga
     4441 tctgggggat aaccaagatg ccgaacagga tcggtgccgc ggccacgccc tcctcgacca
     4501 cgtacccggg caggtgagcc agagcgaatg ccgcccccgt tacgacagcg gctctcatcg
     4561 gactgaaccg atcctgcatc cgatcgagaa ggaatcccgt ccagccgacc tcttcggcaa
     4621 cgttcgagaa aacgatgatc accagaagat gaggcaggac ggtggtgaac atgaggggcc
     4681 acctctgaac gatcaagctc agcggttcgg aaccgtaggc cagggaagca agaaggatgg
     4741 tgcccaccgg aacgcccaag agcgcaacca ggtaccacct tcccggcact cgccaccgca
     4801 gacaccggcg cgcaagatca cgggcacccg cacgacggga caaggcccac accacaatga
     4861 acgcgggcag cgcgctacct aggaaatgac caagccagtc gtaaggagcc tgatcgaaag
     4921 ggagaaggtc gcgtctagtg agggcaggaa acaaaccgac aacgaccgtg accccatagg
     4981 ccatgaccag gaacgccgcg acaggtcgct tggcgatgcc gggggctgct actgccatgc
     5041 gggcgaacct cccgccgccg gctcctatca tccgccacca ggtgacgacc gtctgaggaa
     5101 caggaaatcg tttagtccgg tgtgtaacct ttgtccgctt gctctgatct aggaattgga
     5161 aggggttcat atgcgcaaga caaggttcgc ggtgctggga gtgattgtgg ccgtaggcag
     5221 tctgacgact tttgcctcgc cggctcatgc ctgtaacgag gtcggcgatc cggcctgcac
     5281 ggtgggaggc gccgtgaacg cgtcggcttg cgtggcctcg ttggtcgcgg agagcgcaaa
     5341 aaccggcgaa ccgatcaaca agcacgagtt ggcggactgc ttcaatcaga cgaactaacc
     5401 gttgatgtct tcgtctttga tctttggtcc tggctccgat tccgacggct gattgtcggt
     5461 tactcgacca gcgactcttc gcgcatgccg tacggataga agtactcaag gaagggttcg
     5521 ggatcgccgt cccattcgta gcttcggagc tgatccggac tccggcgtcc tgaaagcgcg
     5581 cggaacagtt cgaatcgatc cgtccgcaaa gtggtgatgg gttcgtcgcc gcccgcgacc
     5641 cagcgcttgc cggaggcttc gaccccaagc gcgtcgccct cctgcatccg gatacggaga
     5701 tccaccgccg ccatgtacgc cgagcttccg atcttgacgg gagggctttc acggtcgcgc
     5761 tcgacaccga aagctccgaa caggtcctgc tgatgcacgc cgagatcggt cacgatgacg
     5821 gtatccgcaa aggccgtgac cccatcgggc cactcgttct cgcctcgcaa gatcgaaaca
     5881 aggtcgggag tgagctcgtt ccactccgcg atgatctcgt cgagcgatcg ttcgtcacga
     5941 gcttcgagtt gtgactgagt ccatttgttc aggtccacga tcgcgtcggc gtccccgaac
     6001 gagcggaaga attcgcgtgg gaagtcgccg cgcaagatgc actcaacgtc tccgacgagg
     6061 tgcgagatga cgtcacgcac cctccactcc ggcgtcgcgg gaaccgggcg gtcctgttcg
     6121 gtcgcatgtt cacgcaccaa ctcgatcact gaggaccgcg ttgtttcgta gacgtcgcca
     6181 agatctccca tgcttagccc ctttccgccc ccgtcacagt tgtgcgaccg gaactccatc
     6241 cagtgttgga acgagctgat agctgatctt ttcggggtcg atccccagcg cagccgcatc
     6301 gttggcggtc aggcgctcga tcagatcttt ggacgcgagg gtcccgtgag ggtcggcgca
     6361 ctcctggatc cgcgcgcatt cgttcacagc gtcccccaat gccgtgacgt ccaggcgccc
     6421 accgggtacg agctgcccga tgtagagcga actcccccag tgcaatccga tccgcatcaa
     6481 acacgacgtg tcgagtgcct caccgaagac gtcttgtgaa cggtcatgga tctgtcgcgc
     6541 ggtgcgaagt gcggcacttg ccgtcgcaga cggagaaccc agatcggatt cgaggaagaa
     6601 cgccgaagca ccatcacccg cgtgcttgcc gacgatgccg tgacttcctg cgaccaattg
     6661 gtctatctcc gtccagaggc tacggatcag ccggaaatac cggctggtag agagcgtgcg
     6721 agccagttcg gtcgaaccat gcaggtcgca gaagaggatc gcagcttcat gagagctggg
     6781 ctcgaccagc ttcgccatcc gctcgtacat ggcctcatca ccgcgcgcga g
//
LOCUS       DATMED010000006         5257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2312370, whole genome shotgun sequence.
ACCESSION   DATMED010000006 DATMED010000000
VERSION     DATMED010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312370"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VNP73_00145"
     CDS             complement(<1..257)
                     /locus_tag="VNP73_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HWL64360.1"
                     /translation="MAKIVKFDSEARQGLEAGVNRLADAVRVTLGPRGRNVVLDKKWG
                     APTITNDGVTIAKEIELEDPWENMGAQLVKEVATKTNDVAG"
     gene            complement(321..629)
                     /gene="groES"
                     /locus_tag="VNP73_00150"
     CDS             complement(321..629)
                     /gene="groES"
                     /locus_tag="VNP73_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWL64361.1"
                     /translation="MATATSTKIRPLEDRLLVRPSEGDETLPSGIVIPDTAKEKPQEG
                     TVLAVGPGRRSENTGEVIPLDVKEGDKVIYSKYGGTEVKVEGEDLLILSARDVLAILG
                     "
     gene            complement(806..1909)
                     /gene="tsaD"
                     /locus_tag="VNP73_00155"
     CDS             complement(806..1909)
                     /gene="tsaD"
                     /locus_tag="VNP73_00155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWL64362.1"
                     /translation="MSETFWPADNPAGTATLPSPLAQKQPIADWDEDSLVLGIETSCD
                     ETSAAVVKGGREILSNVIVSQGELHGEYGGIVPEIAARSHVEALTPAIGEALMRADAT
                     FWDLDAVAATLGPGLIGALLVGVAEAKAISAALEIPFVGVNHLEAHIYSSLLADPAAD
                     FPALCLIISGGHTILAHAFGHGSYEILGSTIDDAAGEAFDKVARFLGLEYPGGPEIDR
                     LSLIGNPDAVAFPRAMINEPGFDVSYSGLKTAVINYVRRLEAKGEPVVVEDIAASFQK
                     AAVDVQVAKTLAAVDLVGAERVFLCGGVAANSGLRGGLGEACEAKGVKLFVPPLELCT
                     DNAAMVAACATAMLRRGVYHSLDVSPDPNLPLV"
     gene            complement(1906..2460)
                     /gene="rimI"
                     /locus_tag="VNP73_00160"
     CDS             complement(1906..2460)
                     /gene="rimI"
                     /locus_tag="VNP73_00160"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWL64363.1"
                     /translation="MMALARTPETGPLDDLELTRMRRRHLRKVLSIEARVYPRPWSMS
                     LFLSELTQRSTRTYLVARHRGEVIGYVGMMFTGKEAHITNIAVDPDFHGRKVGSRLLI
                     TVVTEAMARGAETISLEVRVSNIAAQAMYRKFGFDVAGVRKGYYIETNEDALVMVVQD
                     ALSTGYRLVLQRIRGELEDVGDEL"
     gene            complement(2457..3167)
                     /gene="tsaB"
                     /locus_tag="VNP73_00165"
     CDS             complement(2457..3167)
                     /gene="tsaB"
                     /locus_tag="VNP73_00165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWL64364.1"
                     /translation="MLVLGIETSTPVASVAIGSEQGIVASAMVSRGASYNEFILPAVR
                     FCMDEAGLGFRNLGAISVGLGPGLFTGMRVGVATGKALAQTLSLPMIGMSSLDLVAYG
                     VRHSSKTICAILDARRNEVFYSFYRSSPGGVQRMGGYRVDKPGTLAIGLASRPEDVLL
                     VGNGAHLYSDVFSDLGSVVEIGSMGDAFPDARSLVELSLPRLFRQDFDSPYDLVPLYL
                     RQSARRIQWERVRGSRSA"
     gene            complement(3222..3824)
                     /gene="tsaE"
                     /locus_tag="VNP73_00170"
     CDS             complement(3222..3824)
                     /gene="tsaE"
                     /locus_tag="VNP73_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWL64365.1"
                     /translation="MKVLSVTTQSAEETRIVGAALAPTLLPGDVVSLSGDLGAGKTVF
                     VQGLAAALGVESRVTSPTFTIVHEYDGRYPIIHLDIYRLDNYQEVLDLGFEEFLDPSA
                     VILIEWGEAIGPLLPRRFLEVEIRQSNGEVPEELRSLVFKPHGAEWITKIQTMRDTAE
                     TLLDAASEETVEGPRFSVSDEPSPHLKRTVADEPGQEEDN"
     gene            complement(3843..4517)
                     /locus_tag="VNP73_00175"
     CDS             complement(3843..4517)
                     /locus_tag="VNP73_00175"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWL64366.1"
                     /translation="MPPPPPPEAPPRPDITEIERQALSCTRCPLSQGRTQVVWADGNL
                     DSDILFIGEAPGFHEDKQGKPFVGAAGQLLDKLLGEVGLDRTQVAIVNVLKCRPPGNR
                     DPQPNEIESCRPFLEAQLDHMRPRVIITLGNFATRFMIEQPIGITKARGQKFKFRDAC
                     VIPTFHPAAALRGGRFGGMSPIDAIRQDLQLARRVIDEAPPKPEKSEPDPTEPEETES
                     EQLGLF"
     gene            complement(4575..>5257)
                     /locus_tag="VNP73_00180"
     CDS             complement(4575..>5257)
                     /locus_tag="VNP73_00180"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWL64367.1"
                     /translation="TEALAMEARMSLQEYEDFYYNACRVHSDDPIKEWKELAAHHSRL
                     LDWMKGRKEVHLEGEGTDLTLGIADRTFIPADGDANFPDGEIFTGPDETKTNGHVTFT
                     YPAIYGGQAVEGIRLVFEEGRVVDATAEKNEKFLIQTLDTDPGGRVLGELGIGTNFNI
                     QEFTGDILLDEKIGGTVHLAVGASYPETGGKNDSAVHWDMVCDLRRGGRISVDGDVLV
                     ENGKFLVT"
BASE COUNT          937 a         1704 c         1639 g          977 t
ORIGIN      
        1 tcaccggcaa cgtcgttcgt cttcgtcgcc acttccttga cgagctgagc ccccatgttc
       61 tcccacgggt cttcgagctc gatctctttc gcgatggtca cgccgtcgtt ggtgatcgtc
      121 ggcgcgcccc acttcttgtc gaggacgacg ttgcgtcccc gggggccgag cgtcacgcgc
      181 accgcgtcgg caagccggtt gacgccggcc tcgagaccct gccgggcctc ggaatcgaac
      241 ttcacgatct tggccattaa gttttccttt ctttgttcac gggcgcgccg aaaaatgcgc
      301 cgatgtgagc aagagtgtga ctagccgagg atcgctagaa cgtcgcgagc cgacaggatg
      361 agcaggtcct caccctcgac tttgacctcg gtgccgccgt acttggagta gatgaccttg
      421 tcaccctctt ttacgtccaa ggggatgacc tcgccggtgt tctcggagcg acggccgggg
      481 cctacggcca gaacggtgcc ctcctggggc ttctccttgg cggtgtcggg gataacgatg
      541 cccgagggaa gggtctcgtc gccctctgag gggcggacga ggagacggtc ctcgagcggg
      601 cggatcttgg tggaagtcgc agttgccatt cgtacctcct actaggggtg ttagcactcc
      661 taagtcctga gtgctaagcc taccgccgcc ttcgagaacc ggcaaatggg acccgttctc
      721 ttccgccgac ggccgagccc gtcggcgttc ggggtagatg aggactgaaa tcatggccga
      781 acgccgaacg tctcgacgtt cggcgctaaa cgagcggcag gttggggtcc ggggagacgt
      841 cgagcgagtg atagacgccc cgccgcagca tcgccgtggc gcaggccgcc accatcgcgg
      901 cgttgtccgt gcacagctcc agaggcggga cgaacagctt gacccccttg gcctcgcagg
      961 cctcccccag cccgcccctg aggcccgagt tggccgccac tcccccgcac aggaacacgc
     1021 gctcggcccc cacgaggtcc accgccgcca gtgtcttcgc cacctggaca tcgacggccg
     1081 ccttctggaa cgaggcggcg atgtcctcga cgacgaccgg ctcgcccttc gcctcgagcc
     1141 tgcggacgta gttgataacc gcggtcttca gaccggaata ggacacatcg aacccgggct
     1201 cgttgatcat cgcccgaggg aacgcgactg cgtcggggtt tccgatcagc gacaaccggt
     1261 cgatctcggg accgccggga tactccaggc cgaggaagcg tgcgaccttg tcgaacgcct
     1321 cgccggcggc atcgtcgatc gtgcttccaa ggatctcgta agagccgtga ccgaatgcgt
     1381 gcgccaatat ggtgtgccct ccggagatga tcaagcacag cgccgggaag tcggctgcgg
     1441 gatcggcgag caggctcgaa tagatgtgcg cttcaagatg attcaccccc acgaacggga
     1501 tctcgagcgc ggcggagatc gccttcgcct cggcaacccc caccaggagc gcgccgatca
     1561 gacccgggcc gagcgtggcg gcaacagcat cgagatccca gaatgttgca tcggcacgca
     1621 tgagcgcctc gccgatcgcg ggcgtaagcg cttcaacgtg agagcgcgcc gcgatctccg
     1681 gaacgatgcc tccgtattcc ccatgcaact cgccttggga cacgatgacg ttggagagaa
     1741 tctcgcggcc ccctttaacg acggctgctg aggtttcgtc acaagaagtc tcgatgccga
     1801 ggaccaggga atcctcgtcc caatccgcga tgggctgctt ctgggcaagt ggtgatggga
     1861 gcgtcgcagt accggcgggg ttgtcggccg gccagaaggt ctcgctcata actcgtctcc
     1921 tacgtcctcc aactcgccgc ggatccgctg tagcactaag cggtaaccgg tggagagggc
     1981 atcttgcacg accatcacga gggcgtcttc gttcgtttct atgtaatagc ccttgcggac
     2041 ccccgcgacg tcgaaaccga acttccggta catcgcttgg gccgcgatgt tcgacactct
     2101 gacctcgagt gagatcgtct cggcgcctcg agccatcgcc tcggtcacga ccgtgatcag
     2161 taggcgtgac cccaccttcc gtccgtggaa atcgggatcc accgcgatgt tcgtgatgtg
     2221 cgcctctttt ccggtgaaca tcatcccgac gtagccgatg acctcgccgc ggtgcctggc
     2281 aacgagatag gtgcgggtgg atctctgggt gagctcgctt agaaacagcg acatactcca
     2341 cggtcggggg tacacgcgcg cttcgataga caacactttg cggagatgac gccggcgcat
     2401 gcgcgtcaac tcgagatcgt cgagcggccc cgtttcgggt gtgcgagcga gtgccatcat
     2461 gcggacctgc tcccgcgcac gcgctcccat tggatacggc gcgcggactg cctgaggtac
     2521 agcggaacga ggtcgtaagg cgaatcgaag tcctggcgga agaggcgtgg caagctcagc
     2581 tcgaccagag aacgagcgtc ggggaacgcg tcacccatgc tgccgatctc gaccacgctg
     2641 ccgaggtccg agaacacgtc cgagtagaga tgggctccat taccgacaag gaggacgtct
     2701 tcgggtcgcg acgccaagcc gatcgcaagc gtgcccggct tatcgacgcg atagccaccc
     2761 atgcgctgaa cgccacccgg agaggatcgg tagaacgaat agaagacctc gttgcggcgg
     2821 gcatccagga tcgcgcagat cgtcttcgac gagtgccgta ccccgtacgc aaccaggtcg
     2881 aggctggaca tcccgatcat cggcaaagac agggtctggg ccagcgcctt gccggtagcc
     2941 accccaactc gcatccccgt gaagagaccg gggccgagcc cgaccgatat cgccccgagg
     3001 tttctgaacc ccagaccggc ctcgtccatg cagaaccgga ctgcgggcag gatgaactcg
     3061 ttgtaagagg ctccccgcga caccatcgcg ctcgcgacga tcccttgctc ggaaccgatg
     3121 gcaacgctcg ccaccggtgt cgaggtctcg ataccgagaa ccagcatgct ctccctttcg
     3181 gcctctccgg ccccggcggc cccggggaca ggagccggtc tctaattgtc ctcttcttga
     3241 cccggctcgt cggccacggt gcgcttgaga tgggggctcg gctcgtccga aacagagaag
     3301 cgcggcccct caacggtctc ctcggacgct gcatcgagca acgtttctgc tgtgtcccgc
     3361 atggtctgga tcttcgttat ccactcggct ccgtgcggtt tgaagaccag cgagcgcaac
     3421 tcctccggca cctcaccgtt cgattgtcgg atctcgacct ccaggaaacg acggggtagc
     3481 aacggaccga tcgcttcgcc ccactcgatc aggatcacgg cgctcggatc caggaactcc
     3541 tcgaacccga ggtccagcac ctcttgatag ttgtccaacc ggtagatgtc gagatggatg
     3601 atcggatagc ggccgtcgta ctcatgaacg atcgtgaagg tcgggctggt caccctggac
     3661 tcgaccccga gcgcggcggc caagccctga acgaacacgg ttttccccgc cccgagatct
     3721 ccgctgaggc tcacgacatc cccgggaagg agagtggggg caagcgcggc tcccacgatc
     3781 ctggtctcct ccgccgactg agtggtgacg gacaacactt tcatcgagtc gcctcttacg
     3841 gcctagaaga gaccgagttg ctcggactcg gtctcttctg gctcggtggg atctggctcg
     3901 gacttctccg gcttcggtgg agcctcgtcg atcactctgc gggcgagttg caggtcctgg
     3961 cggatcgcat cgatcgggct catcccaccg aaccgaccgc cgcgtagagc ggcggcggga
     4021 tggaacgtgg ggatcacgca ggcgtcgcgg aatttgaact tctgtcccct cgccttcgtg
     4081 atcccgatcg gctgctcgat catgaaccgg gtcgcgaagt tccccaatgt gatgatcacg
     4141 cgcggacgca tgtgatcgag ctgggcctcc aagaaggggc gacacgactc gatctcgttc
     4201 ggctgcgggt cgcggttccc cggcggacgg cacttcagca cgttgacgat cgcaacctga
     4261 gtgcgatcaa gacctacctc gccgaggagc ttgtcgagca actgaccggc cgcccccacg
     4321 aaaggcttgc cctgcttgtc ctcgtggaag cccggcgcct cacctatgaa caggatgtcc
     4381 gaatcgaggt tgccatcggc ccatacgacc tgggtgcggc cctgcgagag agggcaacgg
     4441 gtacagctga gggcctgacg ctcgatctcg gtgatgtcgg gccgcggggg tgcttccggc
     4501 gggggtggtg gcggcatgac ttcgacccta gttagggagg ctccaagggt caaccatcgg
     4561 cgacgttcga gcggctaagt aactagaaac ttgccgttct ctaccagaac gtccccatcg
     4621 accgagatcc ggcccccccg gcggaggtca cacaccatgt cccaatggac ggccgagtcg
     4681 ttcttgccac cggtctccgg gtaggaggca ccgacggcca ggtgaaccgt cccgccgatc
     4741 ttctcgtcga gaaggatgtc gccggtgaac tcctggatgt tgaagttcgt gccgataccc
     4801 agctcgccga gaacgcgccc tcccgggtcg gtgtcgaggg tctggatcaa gaacttctcg
     4861 ttcttctccg cggtcgcgtc gacgacgcgg ccttcttcga acacgagcct gatcccctcg
     4921 accgcttggc ctccgtagat ggcggggtac gtgaaagtca cgtgtccgtt cgtcttcgtc
     4981 tcgtcggggc cggtgaagat ctcgccgtcg gggaagttcg cgtcgccgtc cgccgggatg
     5041 aaggttcgat cggcgatgcc gagtgtgaga tccgtccctt caccctcgag gtggacctcc
     5101 ttccggccct tcatccagtc gagcagacgc gagtggtggg ctgctagctc cttccactcc
     5161 ttgatcggat cgtccgagtg aacgcggcag gcgttgtaat agaagtcctc gtactcctgg
     5221 aggctcatcc gtgcttccat cgccaatgct tcggtcg
//
LOCUS       DATMED010000007         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3845781, whole genome shotgun sequence.
ACCESSION   DATMED010000007 DATMED010000000
VERSION     DATMED010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3845781"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VNP73_00185"
     CDS             <1..430
                     /locus_tag="VNP73_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64368.1"
                     /translation="YLLILSTTFVSAYVLPSIAGTDPSFVKDVIAAGTNETPAGDIGA
                     LGTAIQLQGFAYLAGGLVFGIALYRANVLARWAAALLAVGGVVSVALSFMPDAFYRLL
                     AFPNGIAMIALGYSLWNEKTVVIPEPVPSVSVSEPTPAGV"
     gene            433..1197
                     /locus_tag="VNP73_00190"
     CDS             433..1197
                     /locus_tag="VNP73_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HWL64369.1"
                     /translation="MADTQSMDSTPLDTKRIARAFGIWFLLTWVFAIAARSLFEPAYT
                     DPDFILGAGSDFRVQLGAVFEFLLLIANIATAVVLYPVVKRQSQIGAMGYVTARIMES
                     AFLMVGLLSLVSLVALRQDMAGATGADAAALEATGSALVSVYKFGFLFGPGLVVGFGN
                     GLLLGWLMYSSGLVPRRLAMWGLIGGPSIIVAFGLVLFGVIETGSPAQALFSLPEMVW
                     EAGLPIYCLWKGFRPSPITDTVDIREPAVAPSMVTV"
     gene            1312..>2971
                     /locus_tag="VNP73_00195"
     CDS             1312..>2971
                     /locus_tag="VNP73_00195"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HWL64370.1"
                     /translation="MGDLRTYRWARVAVATALLLVAVAVALPGSATAVTASSNEALGV
                     PALSSFDTLDNGDPVQVRTLSNRADLVSGGDAFVEVVLPTGVDPAAVKVAVDGRDVSG
                     EFAPGGPGLRGLVTGLPLGHSTIAATLPDGSGAALSVKNAPQGGPVFSGPLIEPWTCR
                     NGSDQPDCSQAPTVEYHYQSTEGGGLESYDPANPPDDVATTTTDEGNEVPFIVREETG
                     YSLRDQYRIAALWDPALGESPNPTAENPGFANKLVLTHGASCNTEYQSGSAPDVLLED
                     ALAQGFAVASHALDNAGHNCNMVTQAESLVMTKEMVVERFGPLNYTIGTGCSGGSLVQ
                     QQVANAYPGVYQGITPQCSFTDAWSTGQQYSEYIGLRKYLEDPATFPEHRIVPAQWPA
                     IFGHQNPANQITFTTAIQNSGEPTRSCPGVAQEDVYNENTNPDGVRCTLTDYMRNVFG
                     VYEDGPDAGKARRPIGNVGIQYGLSGLLTFLGSNIGDPTRPPLAPDQFVALNAGIRGT
                     DLDYNPTDERVTADPVAQERVYRSGAANTASNMDTVAIIDLGGPE"
BASE COUNT          495 a          972 c          947 g          557 t
ORIGIN      
        1 ctacctcctc atcctgagca ctactttcgt gtccgcttac gtcctcccct ccatcgcggg
       61 gaccgatccc tcgttcgtca aggacgtcat cgctgccggc acgaacgaga ctcccgccgg
      121 tgacattggg gcgcttggaa ctgcgatcca gttacagggc ttcgcctatt tggctggagg
      181 tctggtgttc ggcatcgcgc tgtaccgagc caacgtcctc gctcgctggg cggctgcgct
      241 tctcgccgtg ggcggcgtcg tctccgtggc gctctcattc atgccggacg cgttctaccg
      301 gttgctggcc ttcccgaacg gcatcgcgat gatcgccctc ggctactcgt tgtggaacga
      361 gaagaccgtc gtcatcccgg agccggtgcc tagcgtcagc gtttccgaac cgacccccgc
      421 gggggtctag agatggcgga cacgcaatcg atggactcga caccgctcga tacgaagagg
      481 atcgcgaggg ccttcggcat ctggttcctg ctcacttggg tattcgcgat agctgctcga
      541 tcgttgttcg agcctgcgta caccgaccct gatttcatcc tgggtgccgg ttcggacttc
      601 cgcgtgcagc tgggagcggt gttcgagttc ctgctgctca tcgccaacat cgctaccgcc
      661 gtcgtgttgt atccggtcgt caagagacag agtcagatcg gagcgatggg gtacgtcaca
      721 gcacgcatca tggagtccgc cttcctcatg gtcggtctcc tgagcctcgt ctccctggtg
      781 gcactgcgcc aagacatggc cggagcgacc ggtgccgacg cggctgcgtt ggaagcgacg
      841 ggtagcgccc tcgtttcggt ctacaagttc ggattcctgt tcggaccggg actcgtggtc
      901 ggcttcggga acggacttct cctcggatgg ctgatgtaca gctcgggtct cgtgccccgt
      961 cgccttgcga tgtggggact gatcggtggt ccgtcgatca tcgtcgcatt cggcctcgtg
     1021 ctgttcggcg tgatcgagac cgggtccccg gcgcaggcct tgttctcctt gccggagatg
     1081 gtgtgggagg ccgggcttcc gatctactgc ctatggaagg gcttcaggcc gagcccgatc
     1141 accgacacgg tggacatccg cgagcccgcc gtcgctccaa gcatggtgac tgtctagcct
     1201 gaggtaggga tgtcctagga agggaatcca cgcttcaagc acgaactcct ccgcaagcga
     1261 gatagctatc tgttgcgcgc cccgagggca gcgcaggaag gggcgacgcg gatgggggat
     1321 ctaaggacgt atcggtgggc tcgagtcgcg gttgcgacgg ccctactcct agtggcggtc
     1381 gcagtcgcgc tgccggggtc ggcgacggcc gtgaccgctt cctcaaacga ggcgctcggt
     1441 gtgcctgcgc tctcgtcctt cgacacgctc gacaacggcg atcccgtcca ggtccggaca
     1501 ttgtcgaacc gtgccgatct cgtgagcggc ggcgacgctt tcgtcgaggt cgttctcccc
     1561 acaggggtcg atccagctgc cgtgaaggtg gccgtcgatg gccgtgacgt ctccggtgag
     1621 ttcgctcccg gtgggccggg gctgcgcggg ttggtgaccg gtctgccact cggccacagc
     1681 accatcgcag cgacgcttcc cgacggctcg ggtgcggccc tgtcggtgaa gaacgcgcct
     1741 cagggcgggc cggtgttctc cgggccgctc atcgagccat ggacttgccg gaacgggtcc
     1801 gaccaaccgg actgtagcca ggcgccgacg gtcgaatacc actaccagtc aaccgaaggg
     1861 ggagggctcg agtcctacga tcccgccaac ccgccagacg acgtcgccac gactactacc
     1921 gacgagggca acgaagttcc tttcatcgtc cgcgaggaga ccggctacag ccttcgcgat
     1981 cagtatcgga tcgccgcgct gtgggatccg gcgctaggtg agtcgccgaa cccgacggca
     2041 gagaacccgg gcttcgccaa caagctcgta ctaacccacg gcgccagctg caacaccgag
     2101 taccagtcgg gttctgcacc cgacgttctc ctcgaggatg cgctggcgca gggtttcgcg
     2161 gttgcgtcgc acgctctcga caacgccgga cacaactgca acatggtcac gcaggccgag
     2221 tcgcttgtga tgaccaagga gatggtcgtc gagcggttcg gaccgcttaa ctacacgatc
     2281 ggtaccggtt gctcgggtgg gtcgctcgtt cagcagcagg tcgcgaacgc gtaccccggg
     2341 gtctaccagg gaatcacacc gcagtgcagc ttcaccgacg catggagcac cggccagcag
     2401 tactcggagt acatcgggct tcgcaagtat ctcgaggatc ccgcgacctt ccccgagcat
     2461 cgcatcgtcc ccgcgcagtg gcctgcgatc ttcggccacc aaaatcccgc taaccagatc
     2521 accttcacga ccgccatcca gaacagcggt gagcccacca ggagttgtcc gggcgtggcg
     2581 caagaggacg tctacaacga gaacaccaac cccgacgggg tccggtgcac gctgaccgac
     2641 tacatgcgta acgtcttcgg tgtctacgag gatgggccgg acgcgggcaa ggcccgccgc
     2701 cccatcggca acgtcggtat ccagtatggg ttgtccggtc tgctcacctt cctgggctcc
     2761 aacatcggcg atccgacgcg gcccccgttg gcgcccgacc agttcgtcgc gctcaacgcg
     2821 gggatccgtg gaaccgacct cgactacaac ccgacggacg agcgtgtgac ggccgatccc
     2881 gtcgcccagg agcgggtgta ccggagtgga gcggccaata ccgcatcgaa catggacacg
     2941 gtagcgatca tcgacctcgg cggtcccgag c
//
LOCUS       DATMED010000008         4330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3076717, whole genome shotgun sequence.
ACCESSION   DATMED010000008 DATMED010000000
VERSION     DATMED010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076717"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2668
                     /gene="ileS"
                     /locus_tag="VNP73_00200"
     CDS             <1..2668
                     /gene="ileS"
                     /locus_tag="VNP73_00200"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWL64371.1"
                     /translation="WILKQLWDKHLLEEDFKVVPYCPRCETSLSSHEQHMTGAYQTVT
                     DPSIYVRFPLDDEPDVSLLVWTTTPWTLLSNMAAAVGEDVDYVTVADPSADGRRLILA
                     RERVEPLLGEGVEILAETKGSDLVGRRYVPPYEFVATVGLAHTVRPGDFVTTEDGSGI
                     VHLAPYGEEDMVVGKRDDLPIVQMIDGTGHVVDGGGDFAGLWVKDADPRIVEDLDAKG
                     KLFRAEDYEHAYPHCWRCGTPLLYFARKDWYIRTSQIRDQLQASNEDTNWQPPTVKYG
                     RFGDWLANNVDWSLSRDRYWGTPLPVWRCSEGHAVCVGSFSELSELSGKDLTGFDPHR
                     PYVDDVTFACPTCGSESRRVASVIDAWFDSGAMPFGQWHYPFENEAEFEKRFPADFIS
                     EGIDQTRGWFYSLLAVSTLVRGRNSYKNVVCLGLILDADGKKMSKSVGNVIDPWTVCE
                     AQGADALRWYMLTAGTAWSARRLSQDLVEEQLRKFLLTLWNTYSFWVTYAALEDFDPA
                     NVTIPVADRSELDRWIVAELDDTIRVVTAALDDFDPTTGGKRLDRFVDDLSNWYVRRS
                     RRRFWKSGRDQDTQSAFLTLWECLVAVAQLTAPYTPFIAEEIYRNLTGPLPDSPPSVH
                     MSDWPPAQDDRADGALRKRVGLARKLVGLGRSARTEAKVRIRQPLRKAFVVIPPTERS
                     DFEAVLDDFANELNVKEVSIAENLGDYVSYEVKPNFKTLGPRFGKDVGKIATALAGSD
                     AAELAAEAQSSGEVHVQLGGDTISLSRTDLDIRIRQKTQFHVATDGPHAVALDLELDA
                     DLVAEGLAREIVRGIQDLRKNSGLAVEDRIELWVASSAQEILDAVTRHRDYIAAEVLA
                     TTVHEGDAPAEVSKDAIDLEQGRVEIGLKRSS"
     gene            complement(2665..3330)
                     /locus_tag="VNP73_00205"
     CDS             complement(2665..3330)
                     /locus_tag="VNP73_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL64372.1"
                     /translation="MSEEMGEKVRVLVVDDHPMWRDGVRADLESSGRAEVVGEASDGG
                     EAIERAREVMPEVVLMDLQLPTVSGVEATRRIIEESPHVRVLVLSASAEEADVLEAVK
                     AGAAGYLLKSSPSSDVVDAVTRVRSGEPVFTPSLAGLVLDEFRRMATKVEGEPNLTPR
                     ENEVLVLVAKGYTYREIGEKLFISTKTVQNHVQNILTKLQMRKRYELMRYALQKGLDR
                     TPD"
     gene            complement(3327..>4330)
                     /locus_tag="VNP73_00210"
     CDS             complement(3327..>4330)
                     /locus_tag="VNP73_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL64373.1"
                     /translation="NRAVLVFDLILCAWLLVASGLVLGEGGILAGRPSFSTGYPISAA
                     LLWGATGGPWYGVGAGAALAVAQLLTRPINGVSFGELTSGQVQNVIGSCVNYIVAGVA
                     IGLVSRVLVRSAEEVQAANAELVRERERAARLAERESMARQIHDSVLQALAFVHKRGS
                     ELSRQPQIESSEVAQLAAMAEQQEGELRALIARDPQEIPRGQASLRELLEAGVRSVEG
                     VETSMSAVGPIWIERSAAEELAAAVRQALENVVEHAGAGRVAVFAEQADGRVIVSVRD
                     DGKGFSYDEAALRAADKVGILKSMKGRVESIGGAMEITTAAGRGTEIEFSVPFEAST"
BASE COUNT          775 a         1462 c         1337 g          756 t
ORIGIN      
        1 gtggatcctc aagcagctgt gggacaagca cctgctcgag gaagacttca aggtcgtccc
       61 ctactgcccg cgctgcgaga cgtcgctgtc cagtcacgag caacacatga ccggggcgta
      121 ccaaaccgtg accgatccgt cgatctacgt gcgcttccct ctcgacgatg aaccggatgt
      181 gtccttactc gtgtggacga ctaccccgtg gacgttgttg tcgaacatgg ccgcggcggt
      241 cggggaagac gtcgactacg taacggtggc ggatccctcc gccgacggca ggcgactcat
      301 cctcgctcgc gagcgggtcg aacccttgct gggcgaaggc gtggagatcc tcgccgaaac
      361 gaagggcagc gacctcgtcg gcaggagata cgtccctccg tacgagttcg ttgcgaccgt
      421 gggccttgcc catacggtcc ggccgggcga cttcgtcacg accgaggacg gttcgggcat
      481 cgtgcacctc gcgccctacg gcgaggaaga catggtggtc ggcaagcgcg acgacctgcc
      541 gatcgttcag atgatcgacg gcacgggcca tgtcgtcgac ggcggtggag acttcgccgg
      601 cctctgggtc aaagacgccg acccccgcat agtcgaggac cttgatgcga agggcaagct
      661 cttccgggcc gaggactacg agcacgcgta cccgcactgc tggcgctgcg gcactccgct
      721 tctctatttc gcgcgcaagg actggtacat ccgcacatcg cagatccgcg accagttgca
      781 agcctccaac gaggacacga actggcaacc gccgaccgtg aagtacggac gcttcgggga
      841 ttggctggcg aacaacgtcg actggtccct gtcgcgagac cggtactggg gcacgccgct
      901 ccccgtatgg cggtgctcgg aaggacacgc agtatgcgtg ggctcgttct cggaactgag
      961 cgagttgtcg gggaaggacc tcaccggctt cgacccgcac agaccgtacg tagacgacgt
     1021 cacgttcgcc tgcccgacct gcgggagcga gtctcgccgg gtcgccagcg tcatcgacgc
     1081 gtggttcgac tccggtgcga tgccgttcgg ccaatggcac tacccgttcg agaacgaggc
     1141 cgagttcgag aaacggttcc cggccgactt catctccgag gggatcgacc agacgcgcgg
     1201 ctggttctac tcgcttcttg cggtctcgac cctcgtgcgc ggtcgcaact cgtacaagaa
     1261 cgttgtttgt ctgggtctga tcctcgacgc cgacggcaag aagatgagca agtcggtggg
     1321 caacgtgatc gatccctgga cggtgtgcga ggcacagggt gcggacgcgt tgcgctggta
     1381 catgctgacc gcggggacgg cgtggtcggc gcgcaggctg tctcaggacc tcgtggagga
     1441 gcagcttcgg aagttcctgt tgactctctg gaacacctat tcgttctggg tcacgtatgc
     1501 cgcgctggaa gacttcgatc ccgcgaacgt gacgatcccg gtcgccgacc gttccgagct
     1561 cgaccgctgg atcgtcgccg agctggacga cacgatccgt gtcgtaaccg cagcactcga
     1621 cgacttcgat ccgacgaccg gtggcaagcg cctggatcgc ttcgtcgatg acctctcgaa
     1681 ctggtacgtc aggcgatcgc gccgccgctt ctggaagtcc ggacgcgacc aggacacgca
     1741 gagcgcgttc ctcacgttat gggaatgcct cgtcgcggtc gcccaactga ccgctcccta
     1801 cacgccgttc atcgcggaag agatctaccg gaacctgacc ggacctctgc cggactctcc
     1861 cccgtccgtg cacatgagcg actggccacc ggcgcaggac gatcgcgccg acggcgccct
     1921 gcgcaagcgg gtgggtctcg ctcgcaagct cgtgggactc ggccgctcgg cccgcacgga
     1981 agcgaaggtg cgcatcaggc agcccttgcg caaagcgttc gtggtgatcc cgccgacaga
     2041 acgatccgat ttcgaagccg ttctcgacga cttcgccaac gagttgaacg ttaaagaggt
     2101 ctcgatcgcg gagaacctcg gtgactacgt ctcgtacgaa gtgaaaccca acttcaagac
     2161 actcggccct cgcttcggga aggacgtcgg caagatcgca accgccctgg ccggatcgga
     2221 cgcggccgag ctcgcggcgg aggcccagag cagtggagag gtccacgtgc aactcggcgg
     2281 cgacacaatc tctttgtctc gcaccgacct cgatatccgg atccggcaga agacacagtt
     2341 ccacgttgcc accgacgggc cccatgcagt ggccttggat ctcgagttgg atgccgatct
     2401 cgtggccgaa gggctcgccc gcgagatcgt gcgcgggatc caggacctcc ggaagaactc
     2461 aggccttgcg gtcgaggacc gcatcgagct ctgggtcgcc tcttcggctc aagagatcct
     2521 cgatgccgtc accagacatc gcgactacat cgccgccgag gtacttgcga ccaccgtgca
     2581 cgaaggagat gcgccggccg aagtatcgaa ggacgcgatc gatctcgagc agggtcgcgt
     2641 cgagatcggg ttgaagcgaa gcagctagtc cggggttcgg tcgagaccct tctgcaacgc
     2701 gtagcgcatc agctcgtagc gtttgcgcat ctgcagcttc gtcaggatgt tctgtacgtg
     2761 gttctgaacc gtcttcgtgg agatgaagag cttctcgccg atctcgcggt aggtgtagcc
     2821 cttcgcgacg aggaccagga cctcgttctc gcgcggcgtt aggttcggct caccttcgac
     2881 ctttgtcgcc atcctgcgga actcgtccag gacgagccct gcgagtgagg gagtgaaaac
     2941 cggctcaccg gagcgcaccc gggtcaccgc atcgacaacg tcggacgacg gcgaactctt
     3001 cagcaggtag ccggccgctc ccgctttcac ggcctcgagc acgtctgcct cctcggccga
     3061 cgcagacagg accagcacgc gtacgtgcgg cgactcctcg atgatcctgc gggtcgcctc
     3121 gaccccggac accgtcggta attgaagatc catgagcacg acctcgggca ttacctcgcg
     3181 cgctcgctcg atcgcctcgc ctccgtccga agcctccccc acgacttcgg ctcgcccgga
     3241 ggactcgaga tcggcgcgca cgccgtcgcg ccacatcggg tgatcgtcga cgacgagaac
     3301 gcgcaccttc tcgcccatct cctcgctcac gtggacgcct cgaacggaac cgagaactcg
     3361 atctcggttc ctcggccggc agcggtcgtg atctccatcg cgccgccgat cgactcgacc
     3421 cttcccttca tgctcttcaa gatccccacc ttgtcggcgg ctctaagggc tgcctcatcg
     3481 taagagaacc ccttcccgtc gtcgcgaacc gagacgatga cccgtccgtc cgcttgctct
     3541 gcgaacacgg cgacgcgacc tgctcccgcg tgttcgacca cgttctccag agcttgtctc
     3601 accgcggccg ccagctcttc ggcggctgat cgctcgatcc agatcggccc aactgcactc
     3661 atcgacgtct cgaccccctc caccgagcgc acaccggctt cgagcaactc cctcagcgag
     3721 gcctggccgc gcggaatctc ctgcggatcg cgcgcgatga gggctcggag ctccccttcc
     3781 tgttgttccg ccatcgcagc gagctgagcg acctcgctac tttctatctg aggctgccgc
     3841 gacagttcgc ttcctcgttt gtgtacgaag gcaagtgcct gcaacacgga gtcgtggatc
     3901 tgccgtgcca tcgactcgcg ctcggccaag cgagccgctc tctcgcgttc ccggacgagc
     3961 tcggcattgg ccgcttgcac ctcttcggcg gaacgcacca gcacgcgcga gaccaagccg
     4021 atcgctacgc cggccacgat gtagttgacg cacgagccga tgacgttctg gacctgaccc
     4081 gaggtgagct caccgaagga gacgccgttg atcggccgcg taagtagctg agcaactgcg
     4141 agcgcggcgc cggcccccac gccgtaccag ggccccccgg ttgcgcccca taggagcgcc
     4201 gcactgatcg ggtaaccggt ggagaacgag ggccggccgg ccaggatccc tccctccccc
     4261 aacacgaggc ccgacgcgac caggagccac gcgcacaaga tcaggtcgaa aacgaggacg
     4321 gctcgattcc
//
LOCUS       DATMED010000009         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_8315, whole genome shotgun sequence.
ACCESSION   DATMED010000009 DATMED010000000
VERSION     DATMED010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8315"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VNP73_00215"
     CDS             complement(<1..804)
                     /locus_tag="VNP73_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64374.1"
                     /translation="MAASNERVQDALAAYLDHLEMGGPEPDTSHLNASEKRELKELTE
                     ALELTEGVALDRGRGESLTARAEAATAEGEALLAQLRDALPPSVRMEADANTLVSHVG
                     GIEIVDRWIVGTFGGRVRVWLLDEAAAQAIEGNVECLADLDRIFHMFPDMSAVALIGR
                     DLSCLIVEPEDCAPQIQVPSGSLVSRRYKRAIQPAVEALPAFLDELTPYWEPMPGFDP
                     DSGLRIDVSEIGDDFVRGAIEKQHGIGERARRGNPKKEALLGLGKKEITA"
     gene            complement(794..1393)
                     /locus_tag="VNP73_00220"
     CDS             complement(794..1393)
                     /locus_tag="VNP73_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64375.1"
                     /translation="MERDPPEPRLPDGIDDDEYRRIRRLMRAVVLRVWRTNHVIAGLD
                     PWDVVDEAWISMAQNSFASEGPFLPHALTVVRNKAVDALRRGETKRAHRSIDAPLAQE
                     VTGSPGADIDYFKNEEQVTAIQRLALVEEGIYSDDILSDLERKAFIAVQLNRKSRAAV
                     GRELEPPLTGQRVGQIVAVAMIKLQSYVKSNEGGRARGR"
     gene            1509..1694
                     /locus_tag="VNP73_00225"
     CDS             1509..1694
                     /locus_tag="VNP73_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64376.1"
                     /translation="MAEERSIKIRELFIEELAEIVGGGGPEDYIPEVGRPDIGLCAHW
                     NSTMACGEEGPPCPSGC"
     gene            complement(1772..1969)
                     /locus_tag="VNP73_00230"
     CDS             complement(1772..1969)
                     /locus_tag="VNP73_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64377.1"
                     /translation="MSKKLRMFLVVGAMTAGMVLPAAPASAGTCQVANPEIDAVVCGT
                     YLTVMRTACTVVEKTVGGGCA"
     gene            2082..>2815
                     /locus_tag="VNP73_00235"
     CDS             2082..>2815
                     /locus_tag="VNP73_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWL64378.1"
                     /translation="MGSRRFWIFLASLLVALPALVSLPPAAADHDVPVLPDVFPKIRT
                     IVAPLASQPAIVEDGTSLRVELDPEIAGEGTPEGLSASLRPSFGSARPSTSLRDVDLQ
                     LGVESTLWPDRTVHAVTFSVPTLEASFVEDLYDLTVSYADQADTQHRAVKVVDAFPKK
                     PTVAVIADPSVGDPRPIQEGAEELVATGSPDALVEKTTKTVGPMNENRWAALDRAIDE
                     INALQPDFVLVAGDLTFALYPRPANV"
BASE COUNT          497 a          911 c          864 g          543 t
ORIGIN      
        1 cgcggtgatc tccttcttgc cgaggccgag cagagcttcc ttcttcggat tgccccggcg
       61 tgctcgctcg ccgatgccgt gctgtttctc gatcgcaccc ctcacgaagt cgtcgcctat
      121 ctcggagacg tcgatcctca aacccgaatc gggatcgaag cccggcatcg gttcccagta
      181 gggggtcagc tcgtccagga acgcaggcag agcctcgacg gcgggctgga tcgcccgctt
      241 gtaacggcga ctcacaagtg acccgctggg gacctggatc tgcggcgcgc agtcttccgg
      301 ttcgacgatc agacaggaga ggtcccgccc gatcagtgca acggcagaca tgtcggggaa
      361 catgtgaaag atccgatcca gatccgccag acactcaacg tttccttcga tcgcctgtgc
      421 cgccgcttca tcgagcagcc acacccgcac ccggcccccg aacgtcccca cgatccaccg
      481 gtcgacgatc tcgatacccc cgacgtgcga gacgagggtg ttcgcgtccg cttccatgcg
      541 gacgctcggt ggaagagcgt cgcgaagttg tgcgagcaga gcttcacctt cggccgtcgc
      601 ggcttcagcg cgcgcggtga gtgactcgcc tcgaccccga tcgagggcaa ctccttcggt
      661 gagctcgaga gcctcggtca actccttgag ttcacgtttc tcggacgcgt tcagatggct
      721 cgtgtcgggt tcgggaccgc ccatctctag gtggtcgagg tacgcggcca gggcatcctg
      781 aacccgttcg ttactagcgg ccacgtgccc tacctccttc gttgctcttt acatacgact
      841 gcagtttgat catcgccacg gccacgatct ggccgacccg ttgccccgtg agcggcggct
      901 ccagctctct gccgacggcg gcccggctct tccgattcag ctggacggct atgaaggctt
      961 ttcgttcgag gtccgagagg atgtcatcgc tgtagatccc ttcttccacc agagcgagtc
     1021 tctggatcgc agtgacttgc tcctcattct tgaaatagtc gatgtcggct cccggggaac
     1081 ctgtcacttc ctgggccagt ggcgcgtcga tcgaacggtg agcccgcttg gtttctccgc
     1141 ggcgaagcgc gtctactgcc ttattgcgga ccacggtcag agcatgcggc aggaacggac
     1201 cttcgctggc gaagctgttc tgggccatcg agatccacgc ttcatcaact acatcccagg
     1261 gatcgaggcc ggcgatcacg tggttcgtcc tccacacccg caatacgacg gcgcgcataa
     1321 gccgtcggat ccgtctgtat tcgtcatcgt caatcccatc cggaagcctg ggctcaggtg
     1381 gatcacgttc cacgtgatgt ctttatcgca gactcccgaa aatcgaaaag ggccgcggcc
     1441 ctcccggcgc gccgaccgca aaagtggaac aatgcaggca aagcgggcac atcctgtaag
     1501 ggggcgacgt ggcggaggag agatcgatca agattcgcga gctgttcatc gaggagcttg
     1561 ccgagatcgt ggggggcggg ggacccgagg actacatccc cgaggtcggc cgacctgaca
     1621 tcggtttgtg tgcgcattgg aactccacga tggcctgcgg cgaggaaggg ccaccctgcc
     1681 catccggctg ctgagctctg atggaacgct gacggagcgc gaaaaaggcc cccggaaggg
     1741 ggcctttttc gttcttcttc cggtctttga gctaggcgca gccgcctccg acggtctttt
     1801 cgaccacggt gcaggcggtg cgcatcaccg tgagatatgt gccacatacg acggcgtcga
     1861 tctcagggtt ggcaacctga caggtgcctg cagaagcagg ggctgccggc aacaccattc
     1921 cggcggtcat ggctccgacg accaggaaca tccttagttt cttgctcact tttcctcccg
     1981 cgcagggcta tccgatgggg gaggtattcc gccaaggcaa gcagattcct ccccgagagg
     2041 gcggcgattc gccttcccgt ccggagggaa agacgaaagg tatgggctca aggcggttct
     2101 ggatcttcct ggcatccttg ctcgtggccc ttcccgccct cgtttcgcta cctccggccg
     2161 cggcggatca cgatgttccg gtcctgccgg acgtcttccc gaagatcagg acgatcgtgg
     2221 ctccactggc gtcgcagccg gcgatcgtgg aggacggcac ctcgctgcgg gtggaactgg
     2281 atcccgagat cgccggtgag gggacacccg aaggactgag tgcctccctt cgtcccagct
     2341 tcggctcggc gcggccctcg acctcgctca gggatgtcga tcttcagctg ggtgtcgaaa
     2401 gcaccctgtg gccggatcga accgtccatg ccgtgacgtt ctcggtcccg acgctcgaag
     2461 cctcttttgt cgaggacctc tacgacctga cggtctcgta tgcagaccaa gccgacacgc
     2521 agcaccgagc cgtcaaagtc gtagacgcgt tcccgaagaa gccaaccgtc gccgtcatcg
     2581 cagatccctc ggttggagat ccccggccga tccaagaggg ggccgaggag ctcgtagcga
     2641 ccgggtcacc ggatgccttg gtcgagaaga ccaccaagac ggtgggcccg atgaacgaga
     2701 atcggtgggc cgctctcgac agggcgatcg acgagatcaa cgccctgcaa cccgacttcg
     2761 tcctcgtggc aggggacctg acgttcgcgc tctacccgcg gccggcgaac gtcga
//
LOCUS       DATMED010000010         3550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3080207, whole genome shotgun sequence.
ACCESSION   DATMED010000010 DATMED010000000
VERSION     DATMED010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080207"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3403
                     /locus_tag="VNP73_00240"
     CDS             <1..3403
                     /locus_tag="VNP73_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWL64379.1"
                     /translation="STAKDPTPGVVTAGNYEDRNTGERNAAMYSQSSRGRSGQPSTYP
                     DVAAPGAAIMSTCRPNLPVCASGATTAHTKDYASLFGTSMAAPHVAGIAAMLYQADPG
                     LTPAQVEDLLLDTAHKFTAGAAYEADPQNPGGTTSFDKGAGLADAVAALRALGLTGSG
                     TSASTVQVVDGDGGDFPGPGALDIVGLNVSNEESGLRYILEVRDIDDAPLPSMSLRVT
                     QNVDGKNFLTNLTVTAAGITAGTGAQATQIVRDTAANTVSFLVPWANLGNPSTNSPAH
                     NVFVSSFVGGVVDVAPGGPGAEVLVAPRHGAPYTVRPTEETAPTPPPTPTPTPTTSTS
                     PSPSPTPSPTGSPTPIPTGDVIFEDEATIELFSPVGVTQAEFLLTCAEPANTQGLDGY
                     VFELPEATSAREVATLTGSENVHDLDMYFYDADCASSGDAAATTAADEIAFVPNGTKW
                     IFAHSFDGVGTTATLKVYQGEAVPEPTPTPTVTPGDFPFGDRGVYPAEPNDPLFPADP
                     DLPEIIGGQWGMRKIKAPEAWQEVQATGHEVKVAVLDTGLDLNHPDFDCPGKVEVIAN
                     SDFIGDGNGPEDGAGHGTHVAGIVGACTNNGEGVVGVAPDATIMPIQVLDADGSGDPL
                     TTLPGGIRAATDAGANVINMSLGTLPVFTPIDVLIEDVFPEVDEAVEYATTHGVVVIA
                     AAGNESFPLCSYPAIIEDIVCVGSTDSRDVNSYFGNFPVNDDDQDTIGAGLMAPGGSG
                     QVFCDVSAENIVSTYAVELDPCDEGFAGYTGLDGTSMAAPHVAGVAALVYDRLGERTE
                     ANGQAVIDALISSTDDLYAPGWDPASGYGRVNALNAVHAVDAVEPPLAAGTTTQITDD
                     SATSGQFSDAATIAAELTDEDGNPISGSELAFELVGSGGTKSWTATTDENGVAAANVD
                     LDLAPGTYTLAAGFSGVADEYEPSADLGSFQIVKEAATLSFGSGSATSGQYSDTAAVS
                     ALLTDDDGTPLAGVEVVYELAGQTATAITGADGVAATTLNLVDQAGAYQLKASYPENA
                     FHQAAQTASDFTIRAEDSRTTLQVPGPGSDRVYRATVVETDGGTGLAGVVVEFLANGN
                     RIGTATTNANGVATITAKFAKKTVYEAVFAGDAYYLRSSARYQEK"
BASE COUNT          654 a         1184 c         1093 g          619 t
ORIGIN      
        1 ttctacggcc aaagacccaa cccccggtgt tgtcaccgcg ggtaactacg aggaccggaa
       61 cacgggcgag cgcaatgccg cgatgtattc acagtcgagt cgcggcaggt cgggtcagcc
      121 ttcgacctat ccggacgtcg cagccccagg cgccgcgatc atgtcgactt gccggccgaa
      181 cctccccgtc tgcgcgagcg gcgcgacgac ggcacacacg aaggattacg cctcattgtt
      241 cggtacgtcc atggcggcac cacacgtcgc cgggatcgca gcgatgctct atcaagctga
      301 tccgggccta accccggcgc aggtggaaga cctcctactt gatacggcac acaaattcac
      361 cgctggtgcg gcttacgaag ccgacccgca gaatccgggt ggcacgactt ctttcgataa
      421 gggagccggg cttgccgacg cggtcgctgc tctcagggca ctgggcctga ccggctcggg
      481 cacctccgcc tcgaccgttc aggtggtcga cggtgacgga ggcgacttcc ccggtcccgg
      541 cgccctggac atcgtcggtt tgaacgtctc gaacgaagag tcgggactcc gctacatcct
      601 cgaggtccgc gacattgacg acgctcctct tccctcgatg agtctgcgcg tgacgcagaa
      661 cgtcgacggt aagaacttcc tgacgaacct caccgtgact gccgcaggca tcaccgcggg
      721 tacgggtgct caggcgaccc agatcgttag ggacaccgca gccaacaccg tttcgttcct
      781 ggtgccgtgg gcgaacctcg ggaatccgtc gaccaactcg ccggcacaca acgttttcgt
      841 ttcttcattc gtgggcggag tcgtcgatgt cgctccgggc gggcctggtg ccgaggtgct
      901 ggtcgctcct cgacacggcg cgccctacac ggtccggccg actgaggaga ccgctcccac
      961 gccccctcca accccaacgc ccactcccac cacatcgaca tcccccagcc cttcaccgac
     1021 cccgagtccc accggctcgc cgacccccat tcctacgggc gatgtgatct tcgaggacga
     1081 ggcgaccatc gaactattca gcccggtcgg tgtgactcag gccgaattcc tactgacgtg
     1141 tgcggagccc gccaatacac aaggcctcga cggctacgtc ttcgagcttc cggaggccac
     1201 ctcggctcgt gaggttgcga ctctcacggg ttctgagaac gtccacgatc tcgacatgta
     1261 cttctacgac gccgactgtg ccagctcggg tgacgccgcc gcgacgacgg cagccgacga
     1321 gatcgccttc gttcccaacg ggacgaagtg gatcttcgct cactcgttcg acggcgttgg
     1381 aaccacggca accctgaagg tgtatcaggg cgaggcggtc cccgagccga ccccgacgcc
     1441 gacggtgacg ccgggcgatt tccccttcgg ggatcgcggt gtctaccccg ccgaaccgaa
     1501 cgaccctctg ttcccggccg accccgacct gcccgagatc atcggcgggc aatggggcat
     1561 gcgcaagatc aaggctcccg aggcgtggca ggaagtccaa gcgactggtc acgaggtcaa
     1621 ggttgcggtt ctcgacaccg gtttggatct caaccatccc gacttcgatt gcccgggcaa
     1681 agttgaggtc atcgcgaaca gtgacttcat cggcgacggc aatggcccgg aggacggtgc
     1741 cggccacggt actcacgtcg ccgggatcgt cggagcctgc acgaacaacg gcgagggtgt
     1801 cgtcggtgtc gctcccgatg caacgatcat gccgatccag gtgctcgacg cggacggctc
     1861 gggcgaccca cttacgacgc ttcccggcgg gatccgcgct gcgaccgatg ccggagccaa
     1921 cgtcatcaac atgtcgttgg ggactctgcc ggtgttcacc ccgatcgacg ttctcatcga
     1981 ggacgtgttc cccgaggttg acgaggcggt cgagtacgcg acgacccacg gcgtcgttgt
     2041 catcgcggcg gcgggcaacg aatcgttccc gctgtgcagt tatcccgcca tcatcgaaga
     2101 catcgtgtgc gtgggatcga cggactcccg tgatgtgaac tcttacttcg gtaacttccc
     2161 ggtcaacgac gacgaccaag acacgatcgg cgccggactg atggcgcccg gtggtagcgg
     2221 ccaagtcttc tgcgacgtta gtgcagagaa catcgtttcc acctatgcgg ttgagctcga
     2281 tccctgcgac gaaggtttcg cgggttatac gggcctcgac ggcacgtcga tggcggcgcc
     2341 ccacgttgcc ggtgtcgcag ccctcgttta cgaccgtctc ggcgagcgga ccgaggccaa
     2401 cgggcaggcc gtgatcgacg cgttgatctc gtcgaccgac gacctctacg cacccggttg
     2461 ggaccctgct tccggatacg ggagagtcaa tgccctcaat gccgttcatg cggttgacgc
     2521 ggtcgagcca ccactggcag cagggaccac gacgcagatc accgatgaca gtgctacctc
     2581 cggacagttc tcggatgcgg cgaccatcgc cgcagagttg accgacgagg acggcaaccc
     2641 gatctcgggc tccgaacttg ctttcgagct cgtaggttcc ggtggcacga agagctggac
     2701 ggcaacgacc gacgagaacg gcgtcgcggc cgcgaacgtc gatctggatc tggcgcccgg
     2761 cacttacacg ctcgccgccg gcttctcggg tgttgcggac gaatacgagc ccagtgcaga
     2821 tctggggtct ttccagatcg tgaaggaagc tgcgaccttg agcttcggct cgggctctgc
     2881 gacgtcgggc caatattcag acaccgctgc cgtatcggca ctgctgaccg acgacgacgg
     2941 aactccgctc gcaggtgtcg aggtcgtcta cgagctggcc ggtcagacgg ccaccgcgat
     3001 cacgggcgcc gatggagtgg cagccacgac tctgaacctt gttgatcaag ccggcgccta
     3061 ccagctgaag gcgagctacc cggagaacgc tttccatcaa gcagctcaga cggcctcgga
     3121 cttcaccata cgcgccgagg actcgcgcac gacgttgcag gtaccgggac ccggaagcga
     3181 tcgggtgtac cgggccaccg tggtcgagac cgacggcggc accggcttag ccggggttgt
     3241 cgtcgagttc ctcgccaacg ggaaccgcat cgggacggcg acgaccaacg ccaacggcgt
     3301 tgcgaccatc acagcgaaat tcgcaaagaa aacggtctac gaggcagtgt tcgcgggcga
     3361 tgcctactac ctgaggtcat cggctcgtta tcaggagaag taacaacctg acccgcgcag
     3421 acggggggcc gcccaggacc ggccccccgt ccactgcctc gtgattcctt ctttgaaggt
     3481 gcccccggtg ggacttgaac ccacactttc caggttttaa gcccggttcc tctgccaatt
     3541 gggatacggg
//
LOCUS       DATMED010000011         3623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5382036, whole genome shotgun sequence.
ACCESSION   DATMED010000011 DATMED010000000
VERSION     DATMED010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5382036"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /gene="mreC"
                     /locus_tag="VNP73_00245"
     CDS             <1..676
                     /gene="mreC"
                     /locus_tag="VNP73_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWL64380.1"
                     /translation="IGNFFSSLGDLGRLRTENSRLQSEVDDLEGRLTEVQSIAEENEE
                     LRSVLDLDESWVTMERVAAQVIGYDPANYKWAVFIDRGKEDGVRKDMAVVNDDGLVGK
                     VIRTQDNFSTVLLLIDPQAGARARIEEEGPTGAVRGNGGDQSLTLNYIDANDGEVSVG
                     QDVLTSGYDLGIYPPSIPIGEISSVKNEGGAIHQDLEVSPWVDFKSLDFVLVLLESGP
                     QLQEGR"
     gene            676..1230
                     /gene="mreD"
                     /locus_tag="VNP73_00250"
     CDS             676..1230
                     /gene="mreD"
                     /locus_tag="VNP73_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWL64381.1"
                     /translation="MVIAKEPRTNFLAEAGLARILAVGILVVLALALQSAVLARMTLL
                     GVIPQLVLVVVVSLAYLQGERAGVVAGFAGGLLQDLLLPQSIVGLTALVYTLIAYGVG
                     YLRQYTTAESVWTPVLAVAIASAIAEVSYSCMAVLLGQPWVSFGDTMKFTGLIVLYDT
                     LLTPFVFPIVKRVSERVGSSRVYG"
     gene            1230..3197
                     /gene="mrdA"
                     /locus_tag="VNP73_00255"
     CDS             1230..3197
                     /gene="mrdA"
                     /locus_tag="VNP73_00255"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWL64382.1"
                     /translation="MVDRPRAGTDRVTLRLAIFGILMVAAFVALFSRLWFLQVLAAGT
                     YETLAEENRVRFVHSEPPRGRILDRNGHPLVENRRSLAVTLDRQILDEPKRRRKVLNR
                     LSKLVDVGVKQIKDRFNDGTVSPYKPVAVVNDIHESDAVFIDENQEDFPGVGIEELPV
                     RHYPNDTVFAAHIVGYVNEISPEQLKSGHFKTVRPPYAPGDIVGHSGVEYNYDRFLRG
                     RPRIEKVIVNSAGEVIGSRLSQEERPGYDLMLAVDGRIQEVAEKALEAGIQAARGGGY
                     QAPGGAAVVMDPNNGDILGMASFPDYNPRVLADGITTKEFKRLNDPDANAWLNRTIQA
                     QRQPGSTYKIVTAGAAMATGVAGPYDAIECPGSRTFPPEGGPGSVTFNNWTSKYFGFI
                     GFPTSLEISCDTFYYELGWRMEERFGPPESRGGDGTERFQKYARKSGFDHETGIDLPN
                     EAEGRVPDLRWCKVNEDIGYCPEGWYPGYTVNMAIGQGDLIVTPLQMAVAYGAIANGG
                     TVWQPRVAAGLATTNDAGEVETVRPIRNRPAAKLPLDEVEIGVISEGLELVVGGANGT
                     ATGAFAGFPMDEFPIAGKTGTAELGETALNDAWFVSYGPTDAPEYVVAVYVERAGHGG
                     ESAAPIARQIYEGIFDLDDVTDVQLGSDASG"
     gene            3211..>3623
                     /locus_tag="VNP73_00260"
     CDS             3211..>3623
                     /locus_tag="VNP73_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWL64383.1"
                     /translation="MRGGVIFGEAVDRIGGEPIGARMRRKAPVRHVDPMLLLVTLMLA
                     SYGALMVFSATIQQQIDAQLDESFYLKRQIAYVVVGLIVMLVVSFFDYRYVRAFAPFV
                     YAATILGLLIVLTPLGEVSKGASRWINLGFFQMQPS"
BASE COUNT          662 a         1104 c         1169 g          688 t
ORIGIN      
        1 catcgggaac ttcttctctt ccctgggcga ccttggccgg ctccggaccg agaactctcg
       61 cctgcaatcc gaggtcgacg atctcgaggg caggctcacc gaggtccagt cgatcgcgga
      121 ggagaacgag gagctacgca gcgtcctgga tctcgacgaa tcctgggtga ccatggagcg
      181 ggtcgcggct caagtgatcg gctatgaccc ggccaactac aagtgggcgg tcttcatcga
      241 tcggggcaag gaagacggcg ttcgcaagga catggccgta gtgaacgacg acggcctcgt
      301 cggcaaggta atccgcaccc aggacaactt ctcgaccgtg ctgcttctga tcgatccgca
      361 agccggtgcc cgcgctcgga tcgaggagga gggtccgacc ggagccgtgc gggggaacgg
      421 tggggaccaa tctctgacgc tcaattacat cgacgcgaac gatggcgagg tttcggtggg
      481 ccaagacgtg ctcacgtccg gctacgacct cggcatctat ccaccgtcga tccccatcgg
      541 cgagatctca tccgtcaaga acgagggcgg tgcgatccat caggatctcg aggtcagtcc
      601 ctgggtcgat ttcaaatcac tcgacttcgt gctcgtgctc ctcgagagcg gcccacagct
      661 ccaggaaggt cgttgatggt gatagcgaag gagccccgga cgaacttcct cgccgaagcg
      721 ggcctcgctc ggatcctggc ggttgggata ctggtggtgc tagccctggc actgcaatcg
      781 gcggttctcg cccggatgac cctcctgggc gtgatccccc agttggtcct cgtcgtggtc
      841 gtcagtctgg cctacctaca aggggaacgg gccggcgtcg ttgccggctt cgcaggtggt
      901 ctcctgcagg atctgttgct cccgcagtcg atcgtcggtc tcacggcgct cgtctacacc
      961 ttgatcgcat acggagtcgg gtatctccgg caatacacga cggccgaatc cgtgtggacg
     1021 cccgtgctcg cggtcgccat cgcatcggct atcgcggagg tctcctactc gtgcatggcc
     1081 gtcctgctcg ggcagccgtg ggtgagcttc ggcgacacga tgaagttcac cggcctgatc
     1141 gtcctctacg acacgctgct tacgccgttc gtattcccga tcgtcaaacg ggtctccgag
     1201 cgggtcggtt catcgcgggt gtacggctga tggtcgaccg accgagagcg ggtacggatc
     1261 gcgtaactct tcgtcttgcc atattcggca tcttgatggt ggccgcgttc gtcgcgctgt
     1321 tctcgcggct ctggttcctc caggtccttg cagccggtac gtacgagact ctcgccgaag
     1381 agaaccgggt gcgcttcgtt cactcggagc ctcctcgcgg tcgcatcctg gaccggaacg
     1441 gacacccgct ggtcgagaac aggcggtcgt tggccgtcac gctcgatcgt cagatcctcg
     1501 acgagccgaa gcgccggcgg aaggttctta accggttgtc gaaactcgtc gacgtcggcg
     1561 tcaaacagat caaagaccgc ttcaatgacg gaacggtctc gccctacaaa cctgtcgccg
     1621 tagtgaacga catccatgag tcggatgcag tgttcatcga tgagaaccag gaggacttcc
     1681 ccggggtagg gatcgaagag ctccccgtgc ggcactaccc gaacgacact gtgttcgccg
     1741 cacacatcgt tggttacgtg aacgagatct cacccgagca gctcaagtcg ggtcatttca
     1801 agaccgttcg gcccccctac gcgcccgggg acatcgtcgg tcactcgggt gtcgagtaca
     1861 actacgatcg tttccttcgc ggccgcccgc ggatcgagaa agtgatcgtc aactccgccg
     1921 gcgaggtgat cggttctcgt ctctcgcaag aagagcggcc gggttacgac ctgatgctgg
     1981 ctgtggacgg acgcatccag gaagtcgccg aaaaagccct cgaagctggg atccaggccg
     2041 cacgcggcgg cggatatcag gctcccggtg gcgccgccgt ggtgatggat ccgaacaacg
     2101 gagacatcct cggcatggcg agcttccccg actacaaccc gcgcgttctt gccgatggga
     2161 tcaccaccaa agagttcaag aggctgaacg atcccgatgc aaacgcgtgg ctcaaccgca
     2221 cgatccaggc gcaacgccaa cccggctcga cctacaagat cgtcacggcg ggtgcagcga
     2281 tggcgaccgg cgtggcgggt ccctacgacg cgatcgaatg tcccggcagt cgaacgttcc
     2341 cgcccgaggg aggaccgggc agcgtcacgt tcaacaactg gacctcgaag tacttcgggt
     2401 tcatcgggtt ccccacgtcg ctggagatct catgcgacac gttctattac gagctggggt
     2461 ggcggatgga ggagcgtttc ggcccgccgg agagtagggg gggcgacggt accgagcgct
     2521 tccagaagta cgcacgtaag tcgggtttcg atcacgagac cgggatcgat cttccgaacg
     2581 aggccgaggg tcgcgtcccc gatctgcgtt ggtgcaaggt caacgaagac atcggctact
     2641 gccccgaggg ttggtatccc ggttacacgg tcaacatggc gatcggacag ggtgacctga
     2701 tcgtcactcc gctgcagatg gccgtcgctt acggcgccat cgcgaacggc ggcaccgttt
     2761 ggcagccacg ggtggcagcg gggttggcga ctacgaacga cgcgggagaa gtcgagaccg
     2821 tcagacccat ccggaaccgg ccggcagcaa agttgccgct tgacgaagtc gagatcggcg
     2881 tcatcagtga aggcctcgag ctcgtcgtag gcggtgcgaa cgggaccgcg acgggggcgt
     2941 tcgcaggctt cccgatggac gagttcccga tcgccggaaa gacgggtacg gccgaacttg
     3001 gcgagaccgc cctgaacgac gcgtggttcg tttcttacgg accaaccgat gctcctgaat
     3061 acgtcgtcgc cgtgtatgtg gagagggccg ggcacggtgg ggagagcgcg gcccccatag
     3121 cgcgccagat ctacgagggg atcttcgacc tcgatgacgt gacggacgtg caactaggaa
     3181 gcgacgcgtc gggatgatcg gggtcatgcg atgaggggcg gggtcatctt cggcgaggcg
     3241 gtcgaccgca tcggcggtga gccgatcggt gcccggatgc gcaggaaggc tcccgtccgg
     3301 catgtggatc cgatgctcct cctggtcact ttgatgctcg cttcctacgg agctctgatg
     3361 gttttctcgg ccacgatcca gcagcagatc gacgcgcagc tcgatgagtc cttctatcta
     3421 aagcgtcaga tcgcctacgt cgtcgtgggc ttgatcgtga tgctcgtagt cagtttcttc
     3481 gactaccgct acgtgcgagc gttcgccccc ttcgtgtacg ccgccacgat cctcgggctg
     3541 ctgatcgtat tgacccctct cggggaggtg tccaagggtg caagcaggtg gatcaacctc
     3601 gggttcttcc agatgcagcc ttc
//
LOCUS       DATMED010000012         3381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_12164, whole genome shotgun sequence.
ACCESSION   DATMED010000012 DATMED010000000
VERSION     DATMED010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12164"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VNP73_00265"
     CDS             complement(<1..2056)
                     /locus_tag="VNP73_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWL64384.1"
                     /translation="MTLARRTITIVFCDVVESTTLGESMDPELLRQVITRYFEEMQRI
                     LASYDGTVEKFIGDAVMGAFGIPVLHEDDALRAVTAAVDMQGAIEGLNRDLKTQRGLQ
                     LQVRIGVNTGEVVTGDPSAAHGFVSGDPVNVAARLQQQSSPGEILIGEQTYQLVRDAI
                     DADDVGALELKGKARPMRAYRVCDVQRGSLGRWRRLDSPLVGRATEMRSLNEAFSLVI
                     EQGSPSLMSVLGHPGTGKTRLTQEFLSEVTGHAHVLKARFLPYGQIGALRPLAESIED
                     YCAIDEKDSVGEVTRKLAGVVEKDDDRDLVVTRLLALLERTAEAPDIQDLFWAFRRFC
                     EAIGRVRPVILFLDDLHWAESSVLDLVEYMSAFARVPLLVLCTARLSLRDEQRPTWGQ
                     GQPTLVLDRLSDDEVKQLCRILTEGCSLPIELENRIVDMAEGNPLYVEELVRALIDAG
                     LMERTESGWQVVGDIADLEIPPSIGALVAARLDRLTSEERTILERASVVGGSFWWKSL
                     NVVLPPELGPQAGRHLHTLMKKELLEADLSLGSDDAFRFKHEVVRRMAYVGLAKELRS
                     ELHERVARWMVGLSSGATSEEQIAFHLEQSVRLRRALGTADDHTEEMARKASALLDAT
                     GQKALNLSDVATAIGVLRRAVDLLPEDGDRGRLSIDLAGALLESGDLASARDELELVL
                     GRP"
     gene            complement(2154..3152)
                     /locus_tag="VNP73_00270"
     CDS             complement(2154..3152)
                     /locus_tag="VNP73_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64385.1"
                     /translation="MAKAAKGRRRGALCLCAFVTMGALITAPGANAAPELDLTNFIPE
                     ELSAALPDDTGLLVHIDFKPSSDHFLNGARLVSMTANVTPAGDIQLGHAVTAADLGIP
                     ADLGIPEDVIIPECSDPGFEPTGYGWDPVAMPIAWQYRARSFRKRLGRNAVLRDLKKA
                     HRAWDRDTTECSEESDSNFGYAYAGRTKRALKLDTVNIVERGRLPSGALATLYQWYRD
                     SQIIESDLRLSKGTQWGTMTPPKPRRHFLTAGGSAFRHARPATARQSSLGRRAKRYRR
                     FVVANVAAHEFGHDAGLEDLGAPHGELTMYASISRGEHEKVTLGAGDLFGTFLLNP"
BASE COUNT          611 a         1084 c         1053 g          633 t
ORIGIN      
        1 ccggccggcc caggacaagc tcgagttcgt cgcgcgcgga cgccaggtct ccgctctcca
       61 gcaaggcccc cgcaaggtcg atgctcagcc gacctctatc gccgtcctcg gggagaaggt
      121 cgaccgctct ccgcagcacg ccgatggcgg tcgcaacgtc cgacagattc agagccttct
      181 ggccggtggc atccagcagc gcggacgcct tgcgagccat ctcctcggtg tgatcatccg
      241 cggttccgag cgcgcgtcgc aaacggaccg attgctcgag gtggaacgcg atctgctcct
      301 cactggtcgc tcccgagctg agccccacca tccaacgcgc cacccgttcg tgtaattcag
      361 atcgaagctc tttcgccagc cccacgtacg ccatgcgcct cacgacttcg tgcttgaagc
      421 ggaacgcgtc gtcggagccg aggctgaggt cggcctcgag caactccttc ttcatcaatg
      481 tgtgtaggtg tctccccgcc tgggggccga gctccggggg aaggacgacg ttcaatgact
      541 tccaccagaa ggaaccgcct actacggagg ctcgctcgag gatcgtgcgt tcttcactcg
      601 tcaaacggtc gagcctggcg gcaacgagag ccccgatact cggagggatc tcgaggtcgg
      661 cgatgtcgcc gacgacctgc catcccgact cggtcctctc catcaagccg gcgtcgatca
      721 acgctcgcac cagttcctcg acgtagagcg ggttgccttc cgccatgtcg acgatccggt
      781 tctcgagttc gatcggcaag gagcaaccct cggtcaggat ccggcacagt tgtttgacct
      841 catcgtccga gaggcgatcg aggacgaggg tcggttgtcc ctgtccccac gtcggcctct
      901 gttcatcacg gagggacaac cgggccgtgc acaggacgag cagaggtacc cgtgcgaagg
      961 ccgacatgta ctcgaccagg tcgagaacgc tcgattccgc ccagtgaagg tcgtcgagga
     1021 acaggatcac ggggcgaact cttcctatcg cttcacagaa tctgcggaac gcccagaaca
     1081 ggtcctggat gtcgggtgct tcggccgtac gttccagcaa ggcgagtagc cgcgtcacca
     1141 ccagatcccg atcgtcgtcc ttctcgacga cgccggcgag cttccgggtg acctccccga
     1201 ccgagtcctt ctcgtcgatg gcgcagtagt cctcgatcga ctcggccaac ggccgtaagg
     1261 ctccgatctg tccgtaggga aggaaacgag ccttgaggac gtgagcgtgc ccggtgacct
     1321 cgctcaagaa ctcttgtgtc aggcgggtct tgccggtacc gggatgcccg aggacggaca
     1381 taagggaagg tgaaccttgc tctatcacca aggagaaggc ttcgttcagc gatcgcatct
     1441 cggtagctcg ccctacgagc ggggagtcca gccggcgcca tctacccagg cttcccctct
     1501 gcacgtcgca cacccggtaa gcgcgcatcg gccgcgcctt gcccttgagc tccagggcgc
     1561 cgacgtcatc ggcatcgata gcgtctcgca ccaattgata ggtctgttcg ccgatcagga
     1621 tctctccggg cgaggactgt tgctgcaagc gggccgcgac gttgacggga tcaccgctga
     1681 cgaatccgtg tgcagcggaa gggtccccgg tcacgacctc gcccgtattg actccgatcc
     1741 gaacctgcag ttggagtcct cgttgcgtct tcaggtcgcg attgagccct tcgatcgctc
     1801 cttgcatgtc gaccgcggca gtgacggcac ggagggcgtc gtcctcgtga aggaccggta
     1861 tgccgaacgc tcccatcacc gcgtcgccga tgaacttctc gaccgttccg tcgtaggagg
     1921 caaggatccg ctgcatctcc tcgaagtaac gggtgatcac ctgtcgcagc aactcagggt
     1981 ccatggactc gccgagggtc gtcgactcga cgacgtcaca gaagacgatc gtgatggttc
     2041 ggcgggcgag ggtcacccct tccacctgac cccgcgcgcc cccacacgat tgacagaatc
     2101 ttgcgtcggg ttggttggct gcgccgcagc gcgtgcacga ggtcacgaca gctctaaggg
     2161 ttcagtagga acgttccgaa tagatcgccg gcgccaaggg tcaccttctc gtgctcgcca
     2221 cgcgatatgg acgcgtacat cgtgagttcc ccatgcggag caccgaggtc ctcgaggccc
     2281 gcatcgtgcc cgaactcgtg ggccgccacg ttcgcgacga caaatcgtcg ataacgcttg
     2341 gcgcggcggc ccagcgagct ctggcgggcg gtggcgggtc tcgcgtggcg gaacgcggac
     2401 ccacctgcgg taaggaagtg acgccttggt ttcggtgggg tcatggtgcc ccattgggtg
     2461 cccttcgaaa ggcggagatc gctctcgatg atctgggagt cgcggtacca ctggtagagc
     2521 gtggcgagtg cgccggacgg caaccgtccc ctctcgacga tgttgaccgt atcgagcttc
     2581 agcgctcgtt tggtgcgtcc cgcgtaggcg taaccgaagt tggagtcact ctcttcggaa
     2641 cactcggtcg tgtcgcggtc ccaggcgcga tgggctttct tcagatcgcg tagtacggca
     2701 tttctgccga gcctcttacg gaaagacctg gcccggtatt gccacgcgat cggcatcgcg
     2761 accggatccc acccgtaacc ggtgggttcg aatccggggt cggaacattc ggggatgatg
     2821 acgtcctctg ggatccccag gtccgctggg atccccaggt ccgcggctgt gaccgcgtga
     2881 ccaagctgga tgtctccggc tggagtaacg ttcgccgtca tcgaaacgag gcgagcaccg
     2941 ttcaggaaat gatcgctact cggtttgaag tcgatgtgga cgaggagccc ggtgtcgtcg
     3001 gggagtgccg cgctcaactc ctcggggatg aagttcgtga gatccaattc gggcgccgcg
     3061 tttgcgccgg gtgccgtgat cagcgcgccc atggtcacga acgcgcacag acacaacgcg
     3121 ccgcgccgtc gacccttcgc cgccttcgcc accttgacca ctccccgcag ctagccagtc
     3181 acactgtgtt cctgaggtga ttattgccag ggaatatgag gaaaccgtga aaaatccagg
     3241 aaaaagcgaa aaagtctggc aacttacccc caatcggggg atagccccgc cgctgcaggg
     3301 tggataatcc tggcagcgaa gaatcggggg tgacggtgga tctcactgac ccggccttga
     3361 tcccgtaccg cttgcggacg a
//
LOCUS       DATMED010000013         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4232236, whole genome shotgun sequence.
ACCESSION   DATMED010000013 DATMED010000000
VERSION     DATMED010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4232236"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VNP73_00275"
     CDS             <1..1194
                     /locus_tag="VNP73_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64386.1"
                     /translation="NSLLSFERRRVTAVRLAGHCHVQSVKRRRFETLQMADGAALLVG
                     DTSKTVQESLEVAQLIVESVGNPPTEGTATIEGRTSLKFGISSGGRIQLEHNAILIAT
                     DEATGLTIGGTGEFNIRTIANALTFEGKTAKNSPLLTMSKWAVLQEARGVVRMGTCRQ
                     AQINGDPQRGLTIVGIEQGEGSEASTSGLLLRTFTTPINLTGLQSIASLAASSSVAVP
                     QVTTQLPGHRRERRAITAPQSESDENLRADYAAALARLAESRGAPGAVRTRLAWQEYR
                     MKEATGGRTERSTLRAYRLIGYGERVVQPALLFLGLVIAFTIVSFWGHPWDVSRPGVA
                     RLADVFFNWFLSPLHLLRLGEPVNVQLGPGWLEFLMRIAIAIPFVTAVLALRKYVKRS
                     HADSA"
     gene            complement(1348..2499)
                     /locus_tag="VNP73_00280"
     CDS             complement(1348..2499)
                     /locus_tag="VNP73_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HWL64387.1"
                     /translation="MTDISSQMRSHEDASLRVRFAERKDAAEALGKELEAKGDKVSDA
                     DVQRLKEATAEVMQLADEVRRDEKSAATAFLYSLGGSGGGTSARFGSKAAWADEVAKR
                     VTKTASEFGVKALSTGGIDIPSAVELAVPKPVEPVRVIDLLVSRKPLEGNEFEFLRQT
                     VRTNNAAPVADNATKPTSVYTFEDEFDRARVFAHLTEAIPVRYLDDHEEIKNLLSTQL
                     AEDLLKALESDILVGDGTGEHFTGVATVSGVQAQAFSTDVLTTLRKAHTKLTTSGETP
                     TAWVLNHADLEAIDLTREDGATGGFMAGIDEKVFGGLPTVGSSVVPAGTAYLGDWNQS
                     RLYVREAGRLDADVSGVLFEKNQVKLRYEGRFGFAVLRPNAFIEIDLTA"
BASE COUNT          586 a         1003 c         1029 g          636 t
ORIGIN      
        1 aattctttgc tgtcgttcga acgccgacga gtcactgcgg ttcgcctggc tggacactgt
       61 catgtgcaat ccgtgaaacg caggagattc gagacactcc agatggccga cggcgccgcg
      121 ctccttgtgg gggacacgag caagaccgtt caggagagcc ttgaggtcgc acaactgatc
      181 gtcgaaagcg tgggcaatcc gcctacggag ggcaccgcga ccattgaagg caggacctcg
      241 ctcaagttcg gcatttcgtc aggcggccgg atacaactcg agcacaacgc cattctcatc
      301 gcaacggatg aagcaacagg tctaacgatc ggcggcactg gcgaattcaa catcaggact
      361 atcgcaaatg cgttgacgtt tgaaggcaag acggcgaaga actcgccact cttgacgatg
      421 tcgaaatggg ccgttcttca agaagcgcgg ggcgttgttc ggatggggac ttgtcggcag
      481 gcgcagatca atggagaccc gcaacgtggc ctgaccatcg ttggaattga gcaaggggaa
      541 ggctcagaag catccacatc aggattgctg ctgcgaacgt tcaccacacc gatcaacctc
      601 accggcctcc aatcaattgc gtctctagcg gcgtcatctt ccgtcgcagt accccaggtg
      661 acaactcagc tacctggcca tcgccgcgag agaagggcga tcacggcacc ccagagcgag
      721 tcagatgaga accttcgcgc cgactatgcc gcggcactgg cacggctggc agagtcgcga
      781 ggtgccccgg gcgcggtgcg gacccgattg gcgtggcagg agtatcggat gaaggaggcc
      841 acgggcggcc ggactgagcg gtcgacactt cgggcgtacc gactgatcgg ttatggcgag
      901 cgtgttgtac aacccgccct cctcttcctt gggcttgtca tcgcctttac gatcgtgagc
      961 ttctggggcc acccctggga tgtttctcgc cctggcgtgg cccgcctggc ggacgtgttc
     1021 ttcaactggt tcctgtcgcc gctccacctc cttcgactcg gtgagccagt gaacgtacag
     1081 ctcggacccg gatggttgga gttcctaatg cgaatcgcca tcgcgatccc attcgttaca
     1141 gccgttcttg ccttgaggaa gtacgtgaag cgcagtcatg ccgacagtgc gtgatcactg
     1201 gagcgattcg cccggaacgt ccagggcgag tgcgaggcgc aggcggtgtc atcggtcgaa
     1261 agggaaacct cgaccgcctt gcttcccatg ctcagcgcca tgacaatgca cgctgtgggt
     1321 gagcttgacg cctcgcaccc tggctcatca ggcggtcagg tcgatctcga tgaaagcgtt
     1381 cggtcgcagc acggcgaacc cgaaccggcc ctcgtacctg agcttgacct ggttcttctc
     1441 gaagagcaca ccggacacgt cggcgtcgag ccggcctgcc tcacgcacgt aaagacgcga
     1501 ctgattccag tcaccgagat acgcggtgcc ggcgggtacg acgctcgatc cgaccgtggg
     1561 caggccgccg aagaccttct cgtcgatgcc tgccatgaag ccacccgtcg cgccatcctc
     1621 acgggtgagg tcgatcgcct cgaggtcggc gtggttcaga acccacgcag tcggcgtctc
     1681 acccgacgtc gtcagcttcg tgtgcgcctt ccggagagtc gtcagcacgt ccgtcgagaa
     1741 cgcttgcgcc tgaactcccg agacagtggc gacgccggtg aagtgctcgc cggttccgtc
     1801 gcccaccaag atgtcggact ccagggcctt gagcagatcc tcggccagct gagtcgacag
     1861 caggttcttg atctcctcgt ggtcgtcgag gtagcgaacc ggaatcgcct cggtcagatg
     1921 ggcgaacacc cgcgccctgt cgaactcgtc ctcgaacgtg tagaccgagg tcggcttggt
     1981 ggcgttgtcg gcaaccgggg cggcgttgtt cgtcctgacg gtctggcgca ggaactcgaa
     2041 ctcgttcccc tcgagcggct tgcggctgac gagcaggtcg atcacgcgga cgggctcaac
     2101 gggcttgggg actgccagct cgaccgcgga cggaatgtcg atgccgcctg tcgagagtgc
     2161 cttgacgccg aactcgctgg cggtcttggt cacgcgcttg gcgacctcgt cggcccaagc
     2221 ggccttggag ccgaaccgag cagaggttcc gcctccgctg ccaccaaggc tgtacaggaa
     2281 cgccgtggct gcgctcttct cgtcacggcg gacttcgtcg gcgagctgca tcacttcggc
     2341 tgtggcttct ttgagacgtt gcacgtccgc gtcggaaact ttgtcgccct tggcttcgag
     2401 ctccttgccc agggcttcgg cggcgtcttt gcgttctgcg aaccggacac gcagtgatgc
     2461 gtcttcgtgg cttcgcattt gggacgagat gtcggtcatc agagttctcc tacgaatgaa
     2521 tgcgcctaag cgcggtgggg tggattcggc ggcttgccgg cctcgatgag gcgggggtcg
     2581 cacttctcgc ttgccaccgg cttgccggcc acagagggcg ggggccgtag gggcgattca
     2641 ttgccgaagg acttgccacc ggcttgccag ctgtgaggcg ggggccgtag gggaacttct
     2701 gtattgatac taacaccggt caggacgttc gtccgtctcg gactcgtccg gctcgacgcg
     2761 tgtcgaccag gcgacggggg tgcccaaatc tctacccccc gacgatccat gtagaccgca
     2821 gtcgactcct ttttgcgtgt acgggtctgg gacttttgac gggtctgggc tgtatggcgc
     2881 cggctcctcc cgtcgacaag ctcgacagac gtacttccca gcgggcagcg aacccggtcc
     2941 tcgccagcca agatggccac aacgggagca aggattgtct ggacgccttg gcatcagatc
     3001 accgcctggc gctgttggaa aaaatcggcc gttggatttt gccgtgaata aaagaggtcg
     3061 agtgcggtct agcgcgtgtc tcagcgcgag gagattttct ccccccaccc gtcccaatcg
     3121 tctgtgcgta acgaggccgc gttgaggaga gagagatggc gaaagtgcgg tctacaagca
     3181 cgctcagacg gtggggattc tctcccccgt ccccctgacc tccgtgaacg ttggtcacga
     3241 cgccaccgcc gctc
//
LOCUS       DATMED010000014        11675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3852644, whole genome shotgun sequence.
ACCESSION   DATMED010000014 DATMED010000000
VERSION     DATMED010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3852644"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VNP73_00285"
     CDS             complement(<1..1203)
                     /locus_tag="VNP73_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWL64388.1"
                     /translation="MNEQRDGDDGRLEELSALYRISTLSAFSGDTEAAVGEILRVISE
                     LVSCGRPVLFLFSEPFQEMRLETLGEDNGLKIPLREAGLVRRVFVGGSSELTNDIGTD
                     PEGFRLAEILKARQIAAAPLAIGGRVLGVLAAVDSERGAFTEGDLRLVTILGDRAALT
                     IENSQLLSQLGRQVQELESLQRLSRLLTSADSLESVVGESMRIVTDSVPCEKSAVLLY
                     DEATDSLVAHKPVLGVTDEQADAFVIPMSEPSLAGMVFRTNTALTSNDAQHDAWVSPR
                     VRDLLDMSSFLAVPLASGPRPIGVLASVNATAGVFSEEDERFLSLLGSQVGSVIEAIG
                     ARERERGLIMQLREVDRTRSEFISMLAHELRGPMTTIMGFGYTLRDQGHKLDEEKRNH
                     IVATVVRET"
     gene            complement(1239..1838)
                     /gene="coaE"
                     /locus_tag="VNP73_00290"
     CDS             complement(1239..1838)
                     /gene="coaE"
                     /locus_tag="VNP73_00290"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003956644.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWL64389.1"
                     /translation="MLLVGLTGGIGSGKSTFAALLAERGAQIIDADLIGREALQPGKP
                     AWHSVVDQFGDEVLVPGTMDIDRKRLASIVFQDRPALAALNAIVHPVIFKEIADRLET
                     LKDTDAIVVLDAALLVELGLIDSLDHTIVVVANDAKRVERLISGRGMSRKEIADRMAA
                     QAPVEDLIARADIVVENTGSLEHLAAEAERVYSALKGLN"
     gene            complement(1892..3442)
                     /gene="rpsA"
                     /locus_tag="VNP73_00295"
     CDS             complement(1892..3442)
                     /gene="rpsA"
                     /locus_tag="VNP73_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWL64390.1"
                     /translation="MTDTQDEAAQASGEATIPDGHGGVITRTAPSTSGETFAGGTIVE
                     NDVGDTQEALMAAIEATIKPVNEGDLVSGSIVKIDKDEVLLDIGYKSEGVIPSRELSI
                     RNDVDPSEIVSMGDQLEALVLQKEDKEGRLILSKKRAQYERAWGLIEEKRTKDETVEG
                     PVIEVVKGGLILDIGLRGFLPASLVEMRRVRDLQPYVGKVLECRIIELDKNRNNVVLS
                     RRKFLEESQAEQRQDFLTSLTKGEVRKGVVSSIVNFGAFVDLGGVDGLVHVSELSWKH
                     VDHPSEVVEVGEEVEVEVLDVDLERERVSLSLKATQEDPWRQFARAHEVGDVVEGRVT
                     KLVPFGAFVELDDAIEGLVHISELAEHHVERPEDEVSVHDRVEVRIIDIDLDRRRISL
                     SRKQALKAPEDAAAVDSVPAEGEMVPEVPDMELEDASKMEITPEVADDLVEAAEAAPE
                     EEKEEAEAIAVMSPDAPVEDGEPIEASAPDEPDLDERVKSEEPEVESGEETVEAIVED
                     LKRERGQS"
     gene            3642..4133
                     /locus_tag="VNP73_00300"
     CDS             3642..4133
                     /locus_tag="VNP73_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWL64391.1"
                     /translation="MSKRVIVLATLTALIGLGLFGVASARHGVLITDMDGTQEINSET
                     GEGGAGDADGAGKAKIRLLPDSDQVCFRVSWSNIGAPTASHIHEADRGSNGGVVVTLF
                     SGGDTPLPESISAVNGCAGEVSDELSDRIRNNPKSFYVNVHNPEFPGGAIRGQLRHAP
                     RRR"
     gene            complement(4130..6877)
                     /gene="polA"
                     /locus_tag="VNP73_00305"
     CDS             complement(4130..6877)
                     /gene="polA"
                     /locus_tag="VNP73_00305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWL64392.1"
                     /translation="MPPTQAAQRSKSSPKTSAQKKQQRKKLMLIDGYSLAFRAFYALP
                     EDLQTSDGTYTNAVYGFTSMLIKLLQEEKPDYIATCLDSGRKAERTEEYADYKAGRKE
                     TPDNFSSQVPLIVEVLNKLQIPIYAIDGEEADDIIAYLAKCAPKEGVDVQIVTGDRDF
                     FQLVDDHIHVMYNRRGISDIVEMDPKAVEERYGVPPVKYVDLKALEGDPSDNLPGVPG
                     VGTKTAAKLIQKYGSSEEAVAHADEQTPKLAENLAAHGDQVAINKRLSTLQEVPLEVD
                     ISDLTMGAWDLDEIRELFISLEFRTLLERLMADLPETAEGEGEPFEVEVRVFSTPAAL
                     AELAQELEGADRIALDIVPGTARGLPRSLAFAWGDGEVAFVPIGPEGVSTAELVDSLG
                     PALEETITGATVHGGRAALLACALLGIEVDNVAFDTHIGAYLLDPGAPGYSIEEIARK
                     YSGRELRSASGEAPAEEAQGALDLGGDDTETLGEQASLRALATAEIGDAIRPELDRLG
                     MEELYRTIEHPLIPVLTRMEGLGVRIDLEYLEEMARDLDKKLGVLEADCYELAGENVN
                     LGSPAQLRVLLYDKLGLKTTRRTKTGLSTDARALQQLVGQHLFVEKLLEYREYAKLKN
                     TYVDALPPLVDRATGRVHTTYDQTVAATGRLSSTNPNLMNIPIRTDLGRQIRRAFIPE
                     DGYQFLSVDYSQIELRVMAHLSQDPILVEVFEKGEDIHTATGSRVFGVGADELTVKHR
                     SAAKMINYGLAYGLGASGLAERLNVPKAEAQEIMDTYFEQFGGVTDFLDAVVKQAHAD
                     GFTTTLFGRRRYLPELGSGNPRVRAIGERQALNAPIQGTAADIMKLAMINVDAALRNE
                     GLGTRMILTVHDELVFEVPLDEKGSADELVEREMTGVCDMRVPLAVDMSSGADWSKAK
                     S"
     gene            6903..7580
                     /locus_tag="VNP73_00310"
     CDS             6903..7580
                     /locus_tag="VNP73_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWL64393.1"
                     /translation="MSGPDLRNLNPASKEYLEAIFELKEEGQRVLQARIGERLGLAAA
                     TVSEGVKRLLAEGYIAVEGRDIDLTDQGLTIAEALVRRHRLAERMLTDILGIPWHLCH
                     EQAEDWEKVMTPEVEDAILAKLGGQATCPHGNPIPGTESGVPWSELKSVAAMDTGEQG
                     RLTRLLEDVELDHDVLKYLEDHRLMPGVDLKLVERAPDGTLTLEVESQHVSLGHALAD
                     NLWVVPA"
     gene            complement(7628..8137)
                     /locus_tag="VNP73_00315"
     CDS             complement(7628..8137)
                     /locus_tag="VNP73_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HWL64394.1"
                     /translation="MALNTRVEEAMNDQLQKELQSAYVYLGMSAYCESQSLPGFAGWL
                     RAQFDEEQQHAFRFYTFIIDREGAVDLRQLDAPPTRYKSVVDVFETALAHEKAVTVAI
                     NELYDLVATEKDFASQAWLDWFATEQVEEEKTVGQIVDDLHRIGDDGNGLFILDRSMG
                     ERSSTGEAS"
     gene            8413..>11675
                     /gene="gltB"
                     /locus_tag="VNP73_00320"
     CDS             8413..>11675
                     /gene="gltB"
                     /locus_tag="VNP73_00320"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWL64395.1"
                     /translation="MRYPNQTDRRWARPSAPEERDACGIGFVADARGRSSRSIVESAL
                     EALCRVRHRGAMNADQLTGDGAGLLLPVPRVLLATQQDPDRLGVAMIFMDPERVAETQ
                     AVVAEACAAEELELSDWRLVPTDPSALGDQARGSAPAIYQALVLRPLGSDEDEAERRA
                     YRARRRAERSARSGDLRLYFASFSFRTITYKALCAADQLAAFYPDLADDRCEGWFAIF
                     HQRYSTNTAPTWERAQPFRFLGHNGEINTIRGNVALLRAREGRLGSQDLAPEDLLRPV
                     VDTRSSDSGILDEALELLVRGGRDLRQAAAMLVPPAWEGVLGIDPEVADFFHYHSCLL
                     EPWDGPAGLVFSDGLRVAAALDRNGLRPLRVSICEDGLVACASETGAVPTRGHGMVKR
                     LKIGPGEAFSVDPAEGGVMQDRDIKTRLANRRPYGDWIAEHLQRGDPGDPAATTSDEA
                     MLTRQVASGYQKEEFTVVLRPMAVEGHEPTSSMGDDTAQPPLADYARPIFSFLKQRFA
                     QVTNPPIDHLRERHVMSISTRLGRRAALLQEKPDAAALREYPSPLLFPSGVLELERGG
                     AVVLDATWPLENAPEGMEQRLRELAREAAERVHGGSEVVIVSDRSMNADRVPIPSALA
                     AGAVHHHLVDEGLRSAASIVVDADDPRDSHHIACLLTNGADAVCPRLALQSLAELARR
                     GRLGGEVPFDQAQRNFLSAIEDGLLKIMSKMGISTLDSYRGAQIIEAMGLGHDVVDLC
                     FTGIASALGGLSLEELGADALLRHDAAFAAKPSLANTGFIKFKKGGEYHAMNPDVVDT
                     LHEIVGFERVDQMTDEQRAAHQLQSAARGNPAAYERFARLVNERPPAEPRDLLELVAA
                     PSPVPLDEVEPAAAIARRFSTGAMSHGALSAEAHETLSLALNMIGGLANTGEGGEAPE
                     RYRDARNSGIKQIASGRFGVTPEYLAFANELQIKMAQGSKPGEGGQIPGQKVTVEIAR
                     LRHTVEGVALISPPPHHDIYSIEDLAQLIFDLRQVNPRALVSVKLVAEAGVGLVAAGC
                     VKALADVVHVAGSDGGTGASPLSSIKNAGVPWELGLAEAQQALVAGGLRGRARLRADG
                     "
BASE COUNT         2029 a         3832 c         3644 g         2170 t
ORIGIN      
        1 ggtctcgcgg acgaccgtgg cgacgatgtg attgcgtttc tcttcgtcga gtttgtggcc
       61 ctgatccctg agcgtgtagc cgaaacccat tatcgtggtc atcggcccgc gcaactcgtg
      121 agcgagcatc gagatgaact ccgatcgggt gcgatccacc tctctgagct gcatgatgag
      181 ccctcgctcg cgctctcgtg ctccgatcgc ctcgatcaca cttccgacct gcgagccgag
      241 cagcgatagg aaacgctcgt cctcctccga gaaaacgccc gcggtcgcgt tcaccgacgc
      301 cagaaccccg atcggtcggg gaccgcttgc caacggcacg gccaagaatg aactcatgtc
      361 cagaagatcg cgaacccggg ggctgaccca agcatcgtgc tgtgcgtcgt tcgacgttag
      421 agccgtgttc gtccggaaca ccatgccggc cagtgacggt tccgacatcg ggatgacaaa
      481 ggcatcggcc tgttcgtcgg tcacaccgag gacgggtttg tgggccacaa gcgaatcggt
      541 tgcttcgtcg tacagaagga cggcgctctt ctcgcacggc accgaatcgg tcactatccg
      601 catcgactcc cccactaccg attccaacga gtcggcagac gtcaacaggc gcgacaagcg
      661 ctggagactc tcgagctctt gcacttgtcg ccccagctgc gagagcaact gtgagttctc
      721 gatcgtgaga gcggcccgat ccccgaggat cgtcactaat cgcagatcac cttcggtgaa
      781 ggcgccacgt tccgagtcga ccgcggccag caccccaagg acgcggcccc cgatcgcaag
      841 cggggcggcg gcgatctgac gagccttcag gatctccgcg aggcggaacc cttccggatc
      901 ggttccgatg tcgttcgtta gctcggacga tccaccgacg aagactcgcc tgacgagacc
      961 ggcctcgcgc aatgggatct tcaagccgtt gtcttcgccg agggtctcga gccgcatctc
     1021 ctggaagggc tcactgaaga ggaacagcac gggacgaccg cacgacacca gctctgagat
     1081 gacgcggagg atctcgccga ctgccgcctc ggtatctccg ctgaaggcgc tcagggtgga
     1141 gatccggtaa agcgcgctca gttcctcgag ccgaccatcg tcgccgtcgc gttgctcatt
     1201 cattccagaa gatgttagtc ccaaagggac tttcgtactt aattgagacc tttcagagcc
     1261 gagtacacac gctctgcctc ggcggcgagg tgttcgagtg accccgtgtt ctcgaccacg
     1321 atgtcggccc gtgcgatcag gtcctctacg ggggcctggg ccgccatccg gtcggcgatc
     1381 tccttgcggg acatcccccg cccagagatc aggcgctcga cgcgcttggc atcgttggcg
     1441 acgaccacga tcgtgtggtc gaggctgtcg atgagaccga gttccacgag gagcgcagcg
     1501 tccaggacga cgatcgcatc cgtgtccttc agggtctcga gccggtcggc gatctccttg
     1561 aagatgacgg ggtggacgat cgcgttcagg gcggccaagg caggcctgtc ttgaaagacg
     1621 atcgacgcca gcctcttgcg atcgatgtcc atcgttcccg gcaccaggac ctcatcgccg
     1681 aactggtcga cgacggagtg ccaggccggc ttgccgggtt gcagcgcctc gcgcccgatc
     1741 agatcggcgt cgatgatctg agctccacgt tcggcgagca gcgccgcgaa cgtcgacttt
     1801 ccggaaccga tcccgcctgt gagacctacg agcagcatct agacattcga ctcaccccga
     1861 acgccgaacg tctcgacgtt cggcgggaca attaggactg tccgcgctcc cgcttcagat
     1921 cctcgacgat cgcctcgacg gtctcctcac cggactcgac ctcgggctcc tcgctcttca
     1981 cgcgctcgtc gaggtcgggc tcatcggggg cggaagcttc gatcggctcg ccatcctcga
     2041 ccggcgcgtc gggcgacatg acggcgatcg cctcggcctc ttctttctcc tcttcgggag
     2101 cggcctcggc ggcctcgacc aggtcgtcgg cgacctccgg agtgatctcc atcttggagg
     2161 cgtcctcgag ctccatgtcg ggaacctccg gaaccatctc gccctcggcc ggaacgctgt
     2221 ctactgcggc ggcatcctcg ggcgccttca gcgcctgctt gcgcgacaaa ctgatcctgc
     2281 ggcgatcgag atcgatgtcg atgatgcgga cctcgacccg gtcgtggaca ctcacttcat
     2341 cctccggccg ctcgacatgg tgctcggcga gctcggagat gtgcaccaga ccctcgatcg
     2401 cgtcgtccag ctcgacgaag gccccgaagg gaacgagctt cgttacgcgg ccctcgacga
     2461 cgtcgccgac ctcgtgagcc cgagcgaact gtcgccacgg atcttcctgc gtggccttaa
     2521 gggaaaggga gacgcgctcg cgctcgagat cgacatcgag gacttcgacc tcgacctctt
     2581 ctcctacttc cacgacttcg ctgggatggt cgacgtgctt ccaggacaac tcggaaacgt
     2641 gcacgagacc gtccacaccg ccgagatcca cgaatgcacc gaagttcacg atcgaagaaa
     2701 cgacaccctt gcggacctcg cccttcgtca gagacgtcag gaagtcctgg cgctgttccg
     2761 cctgcgattc ctcgaggaac ttccggcggg acaacacgac gttgttcctg ttcttgtcga
     2821 gttcgatgat gcggcattcg agaaccttgc cgacataggg ctggaggtcc cttacccgcc
     2881 gcatctcgac gagtgaggcc ggcaagaacc cgcgcaaccc gatgtccagg atcaagccac
     2941 ccttgacgac ttcgataacg ggtccttcga cggtctcgtc cttcgtgcgc ttctcttcga
     3001 tgagacccca ggcgcgttcg tactgggcgc gcttcttgga caggatcaga cgcccttctt
     3061 tgtcttcctt ctgaagaacg agagcctcga gctggtcgcc catagagacg atctcggaag
     3121 gatcgacgtc gtttcggatc gatagctcgc gcgaggggat gaccccctcg gacttgtagc
     3181 cgatgtccag gaggacctcg tctttatcga tcttgacgat gcttcccgag acgaggtctc
     3241 cctcgttaac gggcttgatc gtggcttcga tggccgccat gagagcttct tgggtatcgc
     3301 cgacatcgtt ctcgacgatc gtgccgccgg caaaggtttc gccggacgtc gagggggccg
     3361 ttctggtgat cacgccaccg tggccgtctg ggatggtcgc ttctcccgaa gcttgagcag
     3421 cttcgtcttg cgtgtctgtc atggactgag ttattgcctc ctaacgaaaa tcaaccccct
     3481 agaagggggc ggagaaagta taggggctgg tcgcagaacc cctcaaccga accgtccgta
     3541 gtacccccga ggttcgcacg acccgcgatc ccctcgggga tcgcggggag cgaagatggg
     3601 taagtaccgc gcgttctgtt ggtctacaga aggaggaagg catgtctaag cgtgtgatcg
     3661 tcctggctac gcttacggcg ctgatcgggc tgggtctgtt cggggtcgca tcggcccgcc
     3721 acggggtgct gatcaccgac atggatggga cccaggagat caattccgag accggcgaag
     3781 gtggagccgg cgacgccgac ggggcgggta aggccaagat ccggctgctc cccgactccg
     3841 accaggtctg cttccgagtg tcgtggtcga acatcggagc cccgaccgca tcgcacatcc
     3901 acgaggccga cagaggttcg aacggaggcg tggtcgtgac cctgttctcc ggcggcgaca
     3961 caccgcttcc cgaatcgatc agtgcagtga acgggtgtgc cggtgaagtg tcggatgaac
     4021 tctccgatcg catccgtaat aacccgaagt cgttctacgt caacgttcac aacccggagt
     4081 tcccgggagg tgcgatccgc ggacagctgc gacacgcgcc gcgccgccgc tagctcttag
     4141 ctttagacca atcggcgccc gacgacatgt cgacggcaag tggtacgcgc atgtcgcaga
     4201 cgccggtcat ctcacgctcg acgagctcgt cggcggagcc cttctcgtcg agagggactt
     4261 caaagacgag ctcgtcgtgc accgtgagga tcatccttgt ccccaggcct tcgttcctca
     4321 gcgccgcatc gacgttgatc atcgcgagct tcatgatgtc ggccgccgtc ccctggatcg
     4381 gagcattcaa ggcctgacgc tccccgatcg ccctgacccg cgggttgccg gagccgagct
     4441 ccggtaggta gcggcggcgg ccgaaaagcg tcgtcgtgaa cccatctgca tgggcctgct
     4501 tcacgaccgc atccaggaaa tcggtgacgc ctccgaactg ctcgaagtac gtgtccatga
     4561 tctcttgagc ctcggccttg ggcacgttaa gccgttcggc caggccggag gcaccaagcc
     4621 cataagccaa tccgtaattg atcatcttcg ccgccgaccg gtgcttcacc gtgagttcgt
     4681 ctgcacccac accgaacacg cgcgagccgg tcgcggtgtg gatgtcctcc cccttctcga
     4741 agacctcaac caggatcggg tcttgcgaca agtgagccat cactcgcaat tcgatctgcg
     4801 agtaatcgac cgaaaggaat tgatacccgt cctccgggat gaacgccctg cgaatctgac
     4861 gacccagatc cgtccggatc gggatattca tcaggttcgg attcgtactc gacagccgtc
     4921 ccgtagcggc gacggtctgg tcgtaagtcg tgtgcactcg tccggtggcc cgatcgacga
     4981 gcggaggcag cgcgtcgaca taggtgttct tgagtttggc gtactctcga tactcgagaa
     5041 gtttctccac aaataggtgc tgaccgacga gctgctgcaa ggctcgggcg tcagtcgaca
     5101 aacctgtctt cgtacggcgc gtggtcttga gtcccaactt gtcgtacagg agcacgcgta
     5161 gctgcgccgg ggacccgaga ttcacgttct cgccggcaag ctcgtagcaa tccgcctcca
     5221 gtaccccgag cttcttgtcc aagtcgcgtg ccatctcctc gaggtactcg agatctatgc
     5281 gaacgccgag gccttccatc cgtgtcagca ccgggatcag cggatgctcg atcgtcctgt
     5341 agagctcctc catcccgaga cgatcgagtt cgggccggat cgcgtcgccg atctccgccg
     5401 tcgccaaggc tctgagcgac gcttgctcgc cgagggtctc ggtgtcgtcc ccacccagat
     5461 cgagcgcgcc ttgagcctcc tcggcaggcg cttccccgga cgcggatctg agttcgcggc
     5521 ccgagtactt gcgagcgatc tcctcgatcg aataaccggg agcacccgga tccagtaggt
     5581 aggcgccgat gtgggtgtcg aaggcaacgt tgtcgacctc gatgcccaac agggcacacg
     5641 cgagcagcgc ggcccggccc ccatggacgg ttgccccggt gatggtctcc tcgagcgcgg
     5701 ggccgagcga atccacgagc tcggcggtcg agaccccctc cgggccgatc ggaacgaacg
     5761 caacctcacc gtcgccccag gcgaacgcga gcgaacgtgg cagaccgcgc gccgtgccgg
     5821 gaactatgtc gagggcgatc ctgtcggccc cctccagttc ctgcgccagt tcggccagcg
     5881 ccgcgggagt ggagaacacc cgcacctcca cctcgaacgg ctcgccctct ccctcggccg
     5941 tctccggaag atctgccatc aagcgttcca gcaaggttcg gaactcgaga gagatgaaca
     6001 gttccctgat ctcatcgagg tcccatgccc ccatggtcag gtcactgatg tcgacctcca
     6061 gcggaacctc ctgcaaagtc gagagccgct tgttgatcgc gacctggtct ccatgcgcgg
     6121 cgaggttctc ggccagcttc ggcgtctgct cgtcggcgtg cgcgacggcc tcctccgaac
     6181 ttccgtactt ctggatcagc ttggcggccg tcttcgtgcc gacgccgggc acgccgggca
     6241 ggttgtcgga gggatcaccc tcgagtgctt tcagatccac gtacttgacg ggaggcacgc
     6301 cgtaacgctc ctcgaccgcc ttcgggtcca tctcgacgat gtcggagatg ccccggcggt
     6361 tgtacatgac gtggatgtgg tcgtcgacga gttggaagaa gtcccggtcg ccggtgacga
     6421 tctgcacgtc gaccccttcc ttcggcgcac acttggcgag gtaagcgatg atgtcgtcgg
     6481 cctcctcgcc atcgatcgca tagatcggga tctgcagctt gttgagaacc tcgacgatca
     6541 gcgggacctg cgacgagaag ttgtcggggg tctccttccg tcccgccttg tagtcggcgt
     6601 actcctccgt ccgctcggcc ttccggccgc tgtccagaca ggtcgcgatg tagtcgggtt
     6661 tctcttcctg aagcagcttg atgagcatcg atgtgaaccc gtagaccgcg ttcgtgtagg
     6721 tcccgtcgga ggtctgcagg tcctccggga gcgcgtagaa ggcgcggaag gccagcgagt
     6781 agccgtctat gagcatcaac ttcttgcgct gttgtttctt ctgggcggag gtcttggggg
     6841 aagacttaga gcgctgcgcc gcctgggttg gtggcatatc tcttgagttt acgattcggg
     6901 tgatgagtgg ccccgatctt cgcaacctca atcccgcttc gaaggagtac ctcgaggcga
     6961 tcttcgagct gaaggaggag ggccagcgcg tgctccaggc ccggatcgga gagcgtttgg
     7021 ggcttgccgc cgccaccgtc tccgagggcg tgaagcgatt gttggccgag ggctacatcg
     7081 cggtcgaagg ccgggacatc gatctgaccg accaagggct cacgatcgcg gaggccctcg
     7141 tccgcaggca ccgtttggca gaacggatgc ttaccgacat cctcggcatc ccctggcacc
     7201 tatgtcacga gcaggccgaa gactgggaaa aggtgatgac gccggaggtc gaggacgcga
     7261 tcctcgcgaa actcgggggc caagcaacct gcccccacgg caacccgatc ccgggaaccg
     7321 agtcgggcgt tccgtggagc gagctcaagt ccgtcgccgc gatggacacc ggcgagcaag
     7381 gccggttgac gcgacttctc gaagacgtcg agctcgacca cgacgttctc aagtacctcg
     7441 aggatcaccg cttgatgccg ggagtagatc tgaagctggt ggaacgcgca cccgacggaa
     7501 cactcaccct cgaggtcgaa tcgcaacacg tatcgctcgg ccatgcactg gccgacaacc
     7561 tttgggttgt cccggcctaa cggccggggc gcgccggccg aggccgccgc gcgtgggtcg
     7621 tgccggccta gctggcctcg ccggttgagc ttctctcccc catagagcga tcgaggatga
     7681 ataggccgtt cccgtcgtct ccgatccgat gcaggtcgtc gacgatctgg cccacggtct
     7741 tctcctcttc gacctgttcg gtcgcgaacc agtcgagcca cgcctgtgac gcgaagtcct
     7801 tctcggtcgc gacgaggtcg tagagctcgt tgatcgcgac cgtcacggct ttctcgtgcg
     7861 ccagtgccgt ctcgaaaaca tccacaacgg acttgtacct ggtcggagga gcgtccagct
     7921 gccggagatc gaccgcacct tcccgatcga tgatgaacgt gtagaagcgg aacgcgtgct
     7981 gctgctcctc gtcgaactgc gcccggagcc agcccgcaaa ccccggtagg ctttgcgact
     8041 cgcagtaagc cgacatgccc agatagacgt aagccgactg gagctctttc tgtagctgat
     8101 cgttcatcgc ttcttcaacg cgcgtattca gagccatagg aacctcctct tggtgattca
     8161 ctacagcgtg acagttcggg cggctcctgt gttttcgcgg ccctgaatgg acctcgttcg
     8221 gtgcgcctga gcaaagcgct tcggctcggc ggacgtcacg ggcggccggc tcggcgggcg
     8281 cccaggacat tcttgacatg gctgcacgtt ccccgtacaa gggactagac ttccaacaca
     8341 cataagtgag caacggcgct ccggaccggg gcgctttttt cttgccccga accaccacat
     8401 accggaggcg atttgcgcta cccgaaccag accgaccgcc gatgggctcg gccatcggcg
     8461 ccggaagagc gagacgcttg cggcatcggc ttcgtcgccg acgcccgcgg ccggtcgtcg
     8521 cgatccatag tcgagagtgc cctggaggcc ttgtgccggg tccggcaccg gggcgcgatg
     8581 aacgccgatc agctgaccgg agacggggcg ggcctcctgc ttccagtacc ccgggtgctg
     8641 ctggccaccc aacaggatcc cgaccgcctc ggcgtagcga tgatcttcat ggatccggag
     8701 cgcgtagcgg agacgcaagc ggtcgtcgcc gaggcgtgcg cggccgaaga gctagagctg
     8761 tccgactggc gtctcgttcc gaccgatcct tcggctctgg gagaccaggc gcggggatcg
     8821 gcgccggcga tctatcaggc cctcgttctc cggcccttgg gctcggacga ggacgaggcc
     8881 gaacgccggg cttacagagc tcgtcgtaga gccgagcgat cggcgcgctc cggggacctt
     8941 cgcctctact tcgcttcgtt ctcgttccga acgatcacct acaaagccct gtgcgccgcg
     9001 gatcagcttg ccgccttcta tcccgacctt gcggacgatc gctgcgaggg ctggttcgcg
     9061 atcttccatc agcgttactc gacgaacacg gcacccacgt gggagcgtgc gcagccgttc
     9121 cggttcctcg gccacaacgg agagatcaat acgatccgcg gcaatgtggc gttgctgcgc
     9181 gcacgcgagg gtcgattggg ctcgcaggac ctcgcgcccg aagatctgtt gcgccccgtc
     9241 gtggacaccc ggtcgtcgga ctccggcatc ctggacgaag cactggagct tctcgtgcgg
     9301 gggggccggg accttcgtca ggccgcggcg atgctcgttc ctcctgcgtg ggaaggggtc
     9361 ttggggatcg atcccgaggt cgccgacttc ttccactacc actcttgcct cctcgaaccg
     9421 tgggacgggc ccgcgggcct cgtgttctcc gatgggctgc gcgtcgcggc agcgctcgac
     9481 cgcaatggtc tgcggccctt gcgcgtttcc atctgcgagg acgggctggt cgcgtgtgca
     9541 tccgagaccg gtgccgtccc tacacgagga cacggcatgg tcaagcgcct caagatcggg
     9601 ccgggcgagg ccttcagcgt cgaccccgcc gaaggcggag tgatgcagga ccgcgacatc
     9661 aaaacgcgcc ttgccaaccg ccgcccgtac ggagactgga tcgcggaaca cctgcagcgc
     9721 ggtgatcccg gtgatccggc cgccactacg agcgatgagg cgatgctcac tcgccaggtt
     9781 gcatccggct atcagaaaga ggagttcacc gtcgtgcttc gacctatggc cgttgagggt
     9841 cacgagccga cttcttcgat gggcgacgac accgcccagc ctccgctggc cgactacgcg
     9901 cggcccatct tctcgttcct gaaacagcga ttcgcccagg tgaccaaccc gccgatcgac
     9961 cacttgcggg aacgtcacgt catgtcgatc agcacgcgcc tcggccgcag agcggccctc
    10021 ctgcaggaga agcccgacgc cgcggccctg cgcgaatacc catctcccct tctgttccca
    10081 tccggggttc tcgagctcga gcggggcggc gcggtcgttc tggacgcgac gtggccgctc
    10141 gaaaacgccc ccgaaggtat ggagcaacgc ctgcgcgagc tggcgcgcga agctgccgaa
    10201 cgggtccacg gcggaagcga ggtcgtgatc gtgagcgacc ggagcatgaa cgccgaccgg
    10261 gtaccgatcc cgtcggcctt ggctgcggga gccgtgcatc atcacctagt cgacgaaggc
    10321 ttgaggtcgg ccgcaagcat cgtcgtggac gcagacgatc cccgcgacag ccatcacatc
    10381 gcttgtcttc tcacgaacgg tgccgacgcc gtgtgccctc gcctcgcgtt gcagtctctc
    10441 gccgaactcg cacgtcgggg ccgtttgggc ggagaggttc cattcgatca ggctcagcgc
    10501 aatttcctct cggcgatcga ggatggcttg ctcaagatca tgtccaagat gggcatctcg
    10561 accctggact catatcgggg cgcccagatc atcgaggcga tgggcctggg tcacgacgta
    10621 gtggatctgt gcttcaccgg tatcgcatct gccctcggcg gactgtcact cgaggaactc
    10681 ggcgccgacg cgttgcttcg tcatgacgcg gcattcgccg ccaagccatc gcttgcgaat
    10741 acggggttca tcaagttcaa gaagggcggc gagtaccacg ccatgaaccc cgacgtggtg
    10801 gatacgctgc atgagatcgt cggcttcgag cgcgtcgatc agatgaccga cgagcagcgg
    10861 gccgcccacc aattgcagag tgccgctcgg gggaacccgg cggcctacga gaggttcgct
    10921 cgcctcgtca acgaacggcc cccggccgag ccgcgcgacc tcctcgagct ggtggcggcc
    10981 ccatctcccg tccctctgga cgaggtcgag cctgccgccg ccatcgctcg ccgcttctcg
    11041 accggcgcga tgtcgcacgg agctctttcc gcggaggctc acgagacctt gtccttggca
    11101 ctgaacatga tcggcggcct tgccaacacc ggcgagggag gcgaggcacc ggagcgctac
    11161 cgcgatgcgc gcaactccgg catcaagcag attgcttctg ggagattcgg ggtcactccc
    11221 gaatacctcg cgtttgcgaa cgagctgcag atcaagatgg cccaaggctc gaagcccggt
    11281 gagggcggac agatcccggg tcagaaagtc acggtggaga tcgctcgtct cagacacacc
    11341 gtcgagggcg ttgcgttgat ctcgccccca ccgcaccacg acatctactc gatcgaagac
    11401 ctggcgcagc tgatcttcga cctgcgccag gtcaacccgc gtgcgctcgt ctcggtgaag
    11461 ctcgtggccg aggcgggcgt cgggctcgtg gccgccgggt gcgtgaaagc cctggcggac
    11521 gtcgtgcacg tcgccggtag cgacggcggc acgggcgcga gcccgctctc gtcgatcaag
    11581 aacgccggcg ttccctggga gctcgggctc gccgaggcgc agcaggcgct cgtcgcgggc
    11641 ggcctgcgcg gccgcgcgcg gctgcgggcc gacgg
//
LOCUS       DATMED010000015         3325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4232686, whole genome shotgun sequence.
ACCESSION   DATMED010000015 DATMED010000000
VERSION     DATMED010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4232686"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..317)
                     /locus_tag="VNP73_00325"
     CDS             complement(18..317)
                     /locus_tag="VNP73_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64396.1"
                     /translation="MTRTRITSCLLVLSLIGLGAGSVRAQGSPQAEFLFQDSLTSTSG
                     VELENFGTCPGVFSTEQVDGAARRVFAFADGSGLALEPSSDVILGWNQAAVMPLV"
     gene            complement(480..552)
                     /locus_tag="VNP73_00330"
     tRNA            complement(480..552)
                     /locus_tag="VNP73_00330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Thr,seq:cgt)
     gene            complement(584..1741)
                     /gene="holB"
                     /locus_tag="VNP73_00335"
     CDS             complement(584..1741)
                     /gene="holB"
                     /locus_tag="VNP73_00335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HWL64397.1"
                     /translation="MSVWKTLHGSRAAEVLAAHVSSGDVAHAWLLLGPVGSGKRPAAI
                     AMAAALNCSVEPGVGCGTCSSCLRITRRRHPDVHHIVPEGPLIPVDVVREQVIPEASR
                     STFEGRAKVFIIEEADRMNDPAQNALLKTLEEPQPDTVFILLSDREEELLDTIRSRCR
                     VVRMEPVDEGTIVELLRKEGATDEHALLAARLSEGNLERARVLAFDDAAMERRGIWVG
                     AVRRLTSASDALDVAAEIVAEAREASKAAEQQQKDEVTELAEALGEGRGTSGARNALA
                     KRHKRELRRLEEEVLADALGSLASFYRDVLAARSGGEEGVTNLDLLPEIRRWAEADVD
                     EARLLAAAERCVRARASLTKNANVALTIESTLVELVRLVPPRALVGTGAGE"
     gene            complement(1738..>3325)
                     /gene="tmk"
                     /locus_tag="VNP73_00340"
     CDS             complement(1738..>3325)
                     /gene="tmk"
                     /locus_tag="VNP73_00340"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWL64398.1"
                     /translation="THANSLSLIGVYGPWPLSAVVFASLAAFATFLGGHVPALSGLVG
                     NEVALALWLDSLSFAFSGVMIASLVIPASVSRGGRLDLGAIRRDLVEGVKFVAVHKQV
                     RPWMLGIAFTFTAAGAVFSLGVSFAKEVLHGGEKGFAFLIGFLGTGMIIGLLAVGGLA
                     KRIQKDVLFSSSILLTGVSLVALAAVGSLDQAIPIASALGFFGGAAYSTGYSLVHEAT
                     SDELRGRTFSAAYTTIRIGTLVGLGVFPVVADLIGSHRVGLPWGVLDLPGTRSTLMIA
                     GLLAVAGGVVSMRAIGARAPNMRTVPLTARGYFIVFEGGEGAGKSTQMSGAVRWLEAR
                     GESVVTTREPGGTPIGDRVRGILLDPEAAEMDARAEALLYAADRAQHVAQVIRPALEA
                     GKVVVSDRFVDSSLAYQGIARGLGLEEIYRLSEWATGGLVPDVVFYLRLDPKEGLDRV
                     GGERDRIEKEETDFHRRVSAAYAELATRYPHRFVVIDASRSPAEIHKGIISALEVKAR
                     DRVKGIEDAPSLQRPGPLPR"
BASE COUNT          665 a         1147 c          954 g          559 t
ORIGIN      
        1 tcatcttcgg tcccccctta cacgagtggc atgacggcag cttgattcca tcccaaaatc
       61 acatccgacg acggttcgag cgcgaggcca ctgccgtcgg cgaacgcgaa caccctccgg
      121 gccgcgccgt ccacctgttc ggtcgagaac acaccgggac aggttccgaa gttctcgagc
      181 tccacccccg aagtggacgt gagcgaatcc tggaacagga actccgcctg cggtgagcct
      241 tgagccctca ccgaaccggc tcctaagccg atgagactga gtacgagtag gcatgaagtg
      301 atgcgagtgc gggtcatgga tcccccttgt agacgtcagg gacaactcaa cccaagaaga
      361 aactggcaat ccatagtcga aagggactaa atcggcttgc attgctcaaa gcgagccggg
      421 gaggggaatc gaaggagaag acgtagatcc ccctcccgct tcgtctctcg ctgcgcgaga
      481 gccggggagg ggaatcgaac ccctgacctg ctcattacga gtgagccgct ctaccgactg
      541 agctaccccg gcgggtgccg cagattgtag tggtgcggcg cgttcactcc cccgcgccgg
      601 ttcccacgag tgctcggggc ggcacgagcc gcaccaactc caccaatgtc gactcgatcg
      661 tcaacgccac gttcgcgttc ttcgtcaacg aagcacgagc ccgaacgcac cgttccgcag
      721 cggccagcaa cctggcttcg tccacgtccg cctccgccca ccgtctgatc tcggggagca
      781 gatccagatt cgtgaccccc tcttcgccgc cggatctcgc tgcaagcacg tctcggtaga
      841 aagaggcgag cgaccccaag gcatcggcga gcacctcctc ctccaacctg cgcagctcgc
      901 gcttgtgcct cttcgcgagg gcattacgag cgcctgaggt accgcggccc tcaccgagcg
      961 cctcggcaag ctcggtgacc tcgtccttct gctgttgctc cgcggctttg ctggcctcac
     1021 gcgcctcagc cactatctct gctgcaacat caagagcatc gctcgcggag gtcagccttc
     1081 tcaccgctcc cacccatatg cctctgcgct ccatcgctgc atcgtcgaaa gcaagcacgc
     1141 gcgcccgctc caggtttccc tcggagagtc gggccgccaa cagagcgtgt tcgtccgtcg
     1201 caccctcctt gcggaggagc tccacgatcg tcccttcatc gacgggctcc atcctgacga
     1261 cccggcaacg cgatcggatc gtgtcgagaa gctcttcttc tctgtcggac aacaggatga
     1321 agaccgtgtc gggttgcggc tcttcgagcg ttttcagcaa ggcattctgc gccgggtcgt
     1381 tcattcgatc ggcctcttcg atgatgaaaa ctttcgccct accctcgaac gtcgagcggg
     1441 atgcctccgg gatcacctgc tcgcgcacga catcgacggg gatcaggggc ccttccggaa
     1501 cgatgtgatg cacgtccgga tggcgtctgc gtgtgattcg caggcaactg gagcaggtcc
     1561 cgcatcccac gccgggttcg accgagcagt tcagagccgc ggccatggcg atcgcggcgg
     1621 ggcgcttgcc cgaacctacg ggtccaagca agagccacgc gtgagcaaca tcaccagacg
     1681 agacgtgcgc cgcgagaact tctgctgccc ttgacccatg cagggtcttc cacacactca
     1741 tcgaggcaac ggacccggtc gctgcaagga cggcgcatcc tcgatcccct tcacgcgatc
     1801 ccttgccttc acttcgagag ccgagatgat ccctttgtgg atctctgcgg gcgaacgaga
     1861 tgcatcgatc acaacgaatc ggtggggata ccgcgttgct agttccgcat aagcggcgga
     1921 cacgcgccgg tggaagtccg tttcctcctt ctcgatccgg tccctctctc cgcccacccg
     1981 gtccaatcct tccttgggat cgagtcggag gtagaagacg acatccggga cgaggccacc
     2041 cgtggcccac tcgctcaggc gatagatctc ttccagaccg aggccccgcg ctatcccctg
     2101 atacgcgagg gacgaatcta cgaaccggtc ggaaacgacg acttttcccg cttcgagggc
     2161 agggcgtatc acttgagcca catgctgagc acgatcggct gcatagagca gagcctcggc
     2221 acgagcgtcc atctcggcag cttcaggatc gagaaggatc ccgcgcaccc gatctccgat
     2281 cggggttccg cccggctcac gcgtcgtgac gactgactcg ccgcgcgcct cgagccagcg
     2341 caccgcacca gacatctgcg tgctcttgcc ggccccctcg cccccttcga aaacgatgaa
     2401 gtaaccacga gcagttaagg ggaccgttcg catattcggc gcacgcgccc cgatagctct
     2461 catcgacacc accccgccgg ctaccgccag tagccccgcg atcatcaaag tgctgcgagt
     2521 cccgggaagg tcgagcaccc cccacgggag acccacgcga tggcttccga tcagatcggc
     2581 gacgaccggg aacacaccca gtcccaccag ggtgccgatg cggatggtcg tgtaagcagc
     2641 gctgaacgtc ctgcctctga gctcgtcgct cgtcgcctcg tgcaccagcg aataccccgt
     2701 tgaatacgcg gcccccccga agaatcccag cgcgctggcg atagggatcg cctggtcgag
     2761 gcttcctacc gcggccaatg caacgaggct gacgccggtc agaaggatcg acgacgagaa
     2821 caacacgtcc ttctggatcc gtttcgccaa tccacccacg gccagcagtc cgatgatcat
     2881 cccggtaccc agaaaaccga tgaggaaagc gaatcccttc tccccgccgt ggagaacctc
     2941 tttggcgaac gagactccaa gagagaagac cgcgccggct gcggtgaacg tgaacgcgat
     3001 gccgagcatc cacggccgga cctgtttgtg gacggccacg aacttcacgc cctcgacaag
     3061 gtcgcgtctg atcgcaccga gatccagtcg gccaccgcga ctgaccgagg ccgggataac
     3121 caagctcgcg atcatcacgc cggagaaggc aaacgacagt gagtcaagcc acagcgcgag
     3181 ggccacttcg ttccccacca gacctgagag ggccggaaca tgaccgccga ggaaggtggc
     3241 gaacgcagcc agggaggcga acacgaccgc cgacagcggc cacggtccgt aaacgccgat
     3301 caacgatagc gagttcgcat gggtc
//
LOCUS       DATMED010000016         4258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3083431, whole genome shotgun sequence.
ACCESSION   DATMED010000016 DATMED010000000
VERSION     DATMED010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3083431"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VNP73_00345"
     CDS             <1..374
                     /locus_tag="VNP73_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64399.1"
                     /translation="RDWQPQHHDHVWAVERAGTKDIFLNTPTQAGWIERYLTDWTGPR
                     GRLGRLRFRMRSSVYPGQTLTFQGVVGEVTIDDAGTTWADLDIDLTVDGETVTECAAR
                     IAIPKDDDDNPWAKDAGSWKP"
     gene            341..1312
                     /locus_tag="VNP73_00350"
     CDS             341..1312
                     /locus_tag="VNP73_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional MaoC family dehydratase
                     N-terminal/OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWL64400.1"
                     /translation="MGEGRGELETLTQQTLADRLRDEFVGKPIGEPYEARDPVSLPMI
                     RSWCDAMGDRNPVYSDEERARESIHGGIVAPPTMLQRWTMKGLSNEGPPQAGGLGSRL
                     VEALEGAGFTSVVATNCEQEYTRYLRPGDHLTGTTIIKAVSDEKKTALGSGHFIESQI
                     TWTDQTGETVGTQMFRIYYYKQAEKQPSSTAPRPRPAISDDTRFFWEGASGKELLVQR
                     CTSCATLRHPPRPMCANCRSLEWEPAPMSGRGSVYSFVVHHHPPVPGFDLPFVVALVE
                     LEEGVRMVSNLTGVTPEEVSIGMPVEAEFVPVDDELTLPLFAPRSAG"
     gene            1309..1725
                     /locus_tag="VNP73_00355"
     CDS             1309..1725
                     /locus_tag="VNP73_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016910244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWL64401.1"
                     /translation="MNGDLSTLRSGDVAMGDELPPLEIPITATLVVAGAIASGDYQEV
                     HHDAELSKSRGTKDIFMNILTTNGLLGRYVTDWTGPDALLKAVRLRLGAPNYPGDTMT
                     LTGKVTAAAEGEITLEIVGANSLGNHVTATVEVALP"
     gene            1722..2903
                     /locus_tag="VNP73_00360"
     CDS             1722..2903
                     /locus_tag="VNP73_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HWL64402.1"
                     /translation="MTLARKAAIVGIGQTEFSKDSGRTEIRMAVEAVSAALDDAGLSA
                     EDVDGFTTFTMDANEEIEIARSLGVNELTFFSRVHYGGGAACAIVQQAAMAVATGVAE
                     VVVVYRALNGRSWRRFGAGAHNRMPTSPTADMVHYSWYLPFGLVTPASWVAMFAQRAM
                     HELGWTSEDFGRVAVADRRHAANNPDAFFFEQPITLDEHQESRWIVEPLRLLDCCQES
                     DGAVALIVTSAERARSLPRPPAMIEGAAQGSGPDQEGMTSYYRDDLMGLPEMGVVARQ
                     LYAQSGLGPGDINTAVIYDHFTPFVLTQLEEFGFCGRGEAAEFARDGNLEVGGRLPIN
                     TNGGQLGEAYVHGMNGIAEGVRQVRGTSVNQVEDADHVLVTAGTGVPTSGLILGRDGE
                     A"
     gene            2903..3772
                     /locus_tag="VNP73_00365"
     CDS             2903..3772
                     /locus_tag="VNP73_00365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWL64403.1"
                     /translation="MRFGIQTFLTDRSMSPGRLAREVEARGFDSMFMAEHTHIPSARA
                     TPAPLGEPLPEQYWRCLDPFVALTAAALAAPKLRVGTAIALVAQRDEIVTAKEVATID
                     HLTEGRFTFGVGFGWNKEEIEDHGVAYGRRRDVTREKMLAMRQLWEQEEASFEGEFVR
                     LSPSKAWPKPVQDPLPVLVGGGAGPKLFSHIAEYAQGWMPIGGRGVAAALPELHAACE
                     KAGRDPSSLMIVPVGTDPKPPEKLERFQSLGVYEIVLGLPYGLSEDETLRILDGYVKA
                     TEPFRDGSPAPPL"
     gene            3901..>4258
                     /locus_tag="VNP73_00370"
     CDS             3901..>4258
                     /locus_tag="VNP73_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64404.1"
                     /translation="METPIPTSTGGPAWNELGEIEYVLPDELPTGWTVKVATERPGRP
                     VGEWTDHFEVLENRVRKSLLVLGAHHPTSEEDESLLDTAGEIKDLSNDNFAFLLVFAV
                     PHDAATTLPAKSRIRGI"
BASE COUNT          799 a         1361 c         1378 g          720 t
ORIGIN      
        1 gcagggattg gcaaccgcag caccacgacc atgtctgggc cgtggagcgt gcggggacca
       61 aggacatctt cctcaacacc ccaacgcagg ccgggtggat cgagcggtac ctcacggact
      121 ggaccggacc ccgtggccgg ctggggaggc tgcgcttccg catgcgcagc tcggtgtatc
      181 cggggcagac actcaccttc cagggagtgg tcggcgaagt aaccatcgac gacgcgggga
      241 caacctgggc ggacctcgac atcgacctga ccgtggacgg cgagacggtc accgagtgtg
      301 ctgcgcgcat agccatcccg aaggacgacg atgacaaccc gtgggcgaag gacgcgggga
      361 gctggaaacc ctgacgcagc agacgttagc ggatcgtctt cgagacgagt tcgtcggcaa
      421 accgatcggc gagccgtacg aggcgcgcga tcccgtttcc ctaccgatga tccggtcctg
      481 gtgcgatgcg atgggggatc gcaacccggt ttattcggac gaggagcgcg cacgcgagtc
      541 gatccacggt ggcatcgtcg ccccgccgac gatgctgcag cggtggacga tgaagggact
      601 gtccaacgaa ggtccgccgc aggccggggg actcgggtcg cgtctcgtcg aggctcttga
      661 gggggccggg ttcacatccg ttgtcgcaac caactgcgag caggaataca cgcggtactt
      721 gcgcccgggt gaccacctga ccggtacgac gatcatcaaa gccgtctccg atgagaagaa
      781 gacggcgctc ggctccggcc acttcatcga aagccagatc acttggaccg accagacggg
      841 ggagaccgtc ggcacccaga tgttccgcat ctactactac aagcaggcgg aaaagcagcc
      901 gtcgtcgacc gcgccgcgtc ctcgcccggc gatcagcgac gacacgcgtt tcttttggga
      961 gggagcgtca ggcaaggagc tcctcgtgca gcggtgcacc tcgtgcgcaa cgttgcgcca
     1021 tccgccgcga ccgatgtgcg ccaactgccg ttcgctcgag tgggaaccgg cacccatgtc
     1081 cggacgcgga tccgtctact cgttcgtcgt gcaccaccat cctccggtcc ccggcttcga
     1141 tctgcccttc gtcgttgctc tcgtcgaact cgaagagggg gttcggatgg tctccaacct
     1201 gacgggcgtc accccagaag aagtctcgat cggcatgccc gtcgaagccg agttcgtccc
     1261 ggtcgacgat gaactcacgc tcccgctgtt cgcaccccgg agtgccggat gaacggcgac
     1321 ctctctacgc ttcgatccgg ggacgtcgcg atgggcgacg agctgccgcc tttggagatc
     1381 ccgatcaccg cgacgcttgt ggtcgccggg gcgatcgcct cgggggacta ccaggaagtg
     1441 catcacgacg ccgagctctc aaagtcgcgc ggcacgaagg acatcttcat gaacatcctc
     1501 accacgaacg gtctcctcgg ccgctacgta accgattgga ccggcccgga cgcactgctg
     1561 aaggcggttc gcctccggct cggcgcgccg aactatcccg gagacaccat gacgttgacc
     1621 ggaaaagtga cggccgctgc cgaaggcgag atcacgctcg agatcgtcgg ggcgaactcg
     1681 ctcggcaacc acgtgaccgc gaccgtcgag gtggccctcc catgacgctt gcacggaagg
     1741 ccgccatcgt cgggatcggt cagaccgagt tctccaagga ctcggggcga accgagatcc
     1801 ggatggcggt cgaggccgtt agcgcggcgc tggacgatgc gggcctctcc gccgaggacg
     1861 tcgacggctt caccacgttc acgatggatg ccaacgaaga gatcgagatc gcgcgttcgc
     1921 tcggagtgaa cgagctcacg ttcttctcgc gggtgcacta cgggggcggc gccgcctgcg
     1981 cgatcgttca gcaggccgca atggcggtcg cgaccggggt ggcggaagtc gtcgttgtct
     2041 atcgagcgct caacggacgt tcgtggcgtc gtttcggagc cggcgcgcac aaccggatgc
     2101 cgacctcgcc cacggccgac atggtccatt actcgtggta cctgccgttc ggtctcgtca
     2161 cgccggcgtc gtgggtcgca atgttcgctc agcgcgcgat gcacgagctc ggctggacat
     2221 cggaagactt cgggcgagtc gccgtcgccg accgtcgcca cgccgccaat aacccggatg
     2281 ccttcttctt cgagcagccc atcaccctcg acgaacacca ggagtcgcgc tggatcgtcg
     2341 aaccactccg gctgctcgat tgctgccagg agtccgacgg agcggtcgcg ctcatcgtga
     2401 ccagtgccga acgcgcccgc tcgctgcctc gcccgcccgc gatgatcgag ggcgccgccc
     2461 aaggaagcgg tcccgaccag gaagggatga cgtcctatta ccgcgacgac ctgatggggc
     2521 tgccggagat gggcgtcgtt gcgcggcagc tctacgccca atccggtctc ggccccggcg
     2581 acatcaacac tgccgtcatc tacgaccact tcactccctt cgtgctcacc cagctggagg
     2641 aattcgggtt ctgtggccgt ggcgaagcgg ccgagttcgc gcgtgacggc aacctcgaag
     2701 tcggtgggcg gcttccgatc aacaccaacg ggggccagct cggagaggct tacgtgcacg
     2761 ggatgaacgg gatcgccgaa ggggtgcgcc aggttcgggg tacgtcggtc aaccaggtag
     2821 aggacgcaga tcacgttctc gtgacggccg gtacgggggt gccgacgagc gggctgatcc
     2881 tcggccgaga cggggaggcg taatgaggtt cggcatccag acgttcctta ccgatcggtc
     2941 gatgtcgccg ggccggctcg cgcgcgaggt ggaggcgcgt gggttcgact cgatgttcat
     3001 ggccgagcac acgcacatcc cttcggcacg cgccacgccg gcgccgctcg gcgagcccct
     3061 gcccgagcag tactggcgat gtctcgaccc cttcgtcgcg ctcaccgcgg ctgcgctcgc
     3121 cgcccccaag ttgcgcgtcg gtacagcgat cgctctcgta gctcagcgcg acgagatcgt
     3181 caccgcgaaa gaggtggcaa ccatcgatca cctcaccgag ggccggttca cgttcggagt
     3241 cggattcggg tggaacaagg aagagatcga ggaccacggt gtcgcctatg gccgccgccg
     3301 tgacgtgact cgagagaaga tgctcgcgat gcgtcagctc tgggagcaag aggaagcgtc
     3361 tttcgaagga gagttcgtcc ggttgtctcc cagcaaggcg tggcccaagc ctgtgcagga
     3421 tccgcttccg gtccttgtcg gtgggggagc aggcccgaag ctgttcagcc atatcgccga
     3481 atacgcgcaa ggctggatgc cgatcggcgg tcgcggcgtt gcggcggcgc tcccggaact
     3541 ccacgcggct tgcgagaaag ccggtcgcga cccttcgtca ctgatgatcg ttccggttgg
     3601 gacggaccca aaacccccgg agaagctcga gcggttccag tccctcggtg tctacgagat
     3661 cgtgctcggc ctcccgtatg gcctgagcga agacgagacg ctcaggatcc tcgacgggta
     3721 cgtgaaggcg acggagccgt tcagagacgg ctcacctgcc ccacccctgt agacaatgca
     3781 cccagaatga agtctgttca ttcacgttca cggctcgtca ttgcttgctt gatggcaaca
     3841 atcctcgcag cgtgtggaga tgccgatcga tcggagccag agctaagtga gccaagctcc
     3901 atggaaacac caattcccac atccacggga gggccagcct ggaatgaact cggggagatc
     3961 gagtacgtct tgccagacga gttaccaact gggtggacgg taaaggttgc aaccgaacgt
     4021 ccaggccgtc cggtaggcga gtggaccgac cattttgagg tgctagagaa tcgagtcagg
     4081 aaaagtctcc tggtcctggg agcccatcac cccacttccg aagaggatga atcgttatta
     4141 gatactgctg gagaaatcaa ggacttatcc aatgacaact tcgccttcct actcgtcttt
     4201 gcagttccac acgacgcagc aacaactctt ccggccaaat cacgtatccg cgggataa
//
LOCUS       DATMED010000017         5444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_395547, whole genome shotgun sequence.
ACCESSION   DATMED010000017 DATMED010000000
VERSION     DATMED010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395547"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VNP73_00375"
     CDS             <1..571
                     /locus_tag="VNP73_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWL64405.1"
                     /translation="VDTAVNEFGRLDVAHLNAGVGERGLDIATLSPESFHRAIGINIG
                     GVVFGTRAAVNAMSKGGAIIATASLAGLVPYGGDPMYGLTKHAVVGFVRSVAKQLEDR
                     NITINAVCPGFTRTAMLEEMVSVFEQRGFPLMDPEDIAEAVVKIASSEHTGEAFICQP
                     GRIGELYKFRGVPGPRTEGAEGMEPPRPS"
     gene            568..1266
                     /locus_tag="VNP73_00380"
     CDS             568..1266
                     /locus_tag="VNP73_00380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWL64406.1"
                     /translation="MSSPTILWDFDGTLAYRDGLWSGCLAEVLQAQEPGAGITRDDVR
                     PLLKNGFPWHTPDRAHPELDTPEAWWEVVEGLLILACLELGFPEMKARRYAREAHERY
                     IDVAGFRLFDDTLSVLTKLKEEGWRHVILSNHVPELRDIVEGLRLSVLVEDVLSSAVT
                     GYEKPNPEAFRLGREAAGNPDRLWMVGDNPQADVAGAEAIGIPAILVRSKAEGVARVA
                     TDLHEVFELITRMR"
     gene            complement(1250..1636)
                     /locus_tag="VNP73_00385"
     CDS             complement(1250..1636)
                     /locus_tag="VNP73_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014684699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL64407.1"
                     /translation="MGLDRLDHSVIAVSDWERSNAFYRDVVGVEALQQPDGRWLYRIG
                     DQQLSVHGPGVPDSPLRPRVDVQAGGAHVCFTWAGPIEDATAHLESHGVSVEEGPVER
                     EGGRGQGSSVYFRDPDGNLLEFISYG"
     gene            1700..2824
                     /locus_tag="VNP73_00390"
     CDS             1700..2824
                     /locus_tag="VNP73_00390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64408.1"
                     /translation="MTNVPGVFTAEQQELRSMARKFLEETSSSSQVRSLIETAEGFDE
                     GVWKQMAELGWQGIAIPEEYGGSGYTFTELGVLLEEMGRALLPAPFFSSVVLGANAIL
                     NVATDAQKKDILPGIADGSARPTLAFVEDAGRWDQAGIGLEASESKGGYQLTGHKNYV
                     IDGQTATHLIVTARLDGKVAFFLVDPAADGVTKTSLETLDPTRKQARIAFEGTPATLL
                     GDAGAEDALTKTLDQAAVGLAAESVGGAQWCLDTATEHAKTRVQFGRPIGSFQAVKHR
                     CAEMLIEIEMAKSAAYYAAWAAAEDEQELPVAGSLAKAYCSEAFFHAAGTNIQVLGGI
                     GFTWEHDAHLYFRRAKSSEIYLGDADYHRELIADRTGVTA"
     gene            2821..4068
                     /locus_tag="VNP73_00395"
     CDS             2821..4068
                     /locus_tag="VNP73_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64409.1"
                     /translation="MRWRIEDTPEQAEFRKEFLAWVTKNIPAGWAEALEAGDEEKFAK
                     AYSEFNVMEWNSVIGGAGYAAPLWPKEYGGLSGEVWMQNVVREELARLRLPLFGINIL
                     GIGLAGPTLIQHASDEQKKRYLPKILTSDEVWCQLFSEPGAGSDLASLSTRAEKDGDE
                     WVVKGQKVWTSIAEWAKFGMLVARTDPSLPKHEGLTYFICPMKDDNVEVRPLKQITGA
                     SEFNEVYLNEVRIPDSQRVGEVGQGWEIARTTLMNERVALSGLSTSPESFLGGARKDP
                     WKTFVESVPNKSDTLVRQNVARFFIEREAKEMTSFRAAAARRMGEQPGPEGAAGKVFN
                     ADMNQRQTSFAMNAAGPAAIAWHPDDKLADGRARAFLRARGYSIEGGTTEILKNQLAE
                     RILGLPRDPQADKGVPWKDIKRS"
     gene            4044..5252
                     /locus_tag="VNP73_00400"
     CDS             4044..5252
                     /locus_tag="VNP73_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL64410.1"
                     /translation="MEGHQAQLAAAEEEPALDRSRLTRLFAATFCHFVSMGMYIAAVP
                     LFVHFELGASRAIVGLAVGSFFPGAILMRPIIGAGLDSWGRRPFVLVATGIATLTSLL
                     FLFADSVWFVIMLGLLRGFVGAAYYTGAATIATDIGPESRRAEVIALFSLFLYGGFAI
                     GPGIGEWLAENVSFSVVWMTAAGLSALAFAFATGLPETLELTEEEERPPGGRFGLHRA
                     GLAPGLVLVGAASGYTAITGFAPLYAREIGLATSGGLYATFSLTILGFRLVTRKLADR
                     HGRLQIALPGLFMNTLGLATLALFRSAFAAYLAVAIFGIGFALLFPALMAFTADRVAE
                     SQRGSAMTTFTMFFDFGALIGTYVVGLIADNLGFGAGFGLPAAMCALATLGIGARVLF
                     GFGRRQAKTR"
     gene            complement(5234..>5444)
                     /locus_tag="VNP73_00405"
     CDS             complement(5234..>5444)
                     /locus_tag="VNP73_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWL64411.1"
                     /translation="GAKMGINYGTDKVRFTNPVPSGSNVRLKAKLTEAQPKGDGLLYK
                     VGVEIEIEGQEKPAMVGEVLYLVFA"
BASE COUNT          984 a         1664 c         1791 g         1005 t
ORIGIN      
        1 ggtcgacact gcagtgaacg agttcggccg actcgacgtc gcgcacctga acgctggtgt
       61 gggggaacga gggctcgata tcgcgacgct cagtccggaa tcattccacc gagccattgg
      121 gatcaacatc ggtggggtcg tcttcggtac ccgagcggcg gtaaatgcga tgtctaaagg
      181 aggagcgatc attgcaaccg cctcgcttgc agggcttgtg ccttacggcg gcgatccgat
      241 gtacggactc accaagcatg ccgtcgtcgg gttcgttcgt tcggtcgcca aacagctgga
      301 ggacaggaac atcacgatca acgcggtgtg cccggggttc acgagaacgg cgatgctgga
      361 ggagatggtc tctgtcttcg agcagagggg cttcccactg atggacccgg aggacatcgc
      421 cgaggccgtc gtgaagatcg catcatccga gcacaccggc gaggcgttca tttgtcagcc
      481 gggtcggatc ggcgagctct acaagttccg cggcgttccc ggcccccgca ccgaaggtgc
      541 cgaaggcatg gaaccccccc ggcccagttg agctccccca cgatcttgtg ggatttcgac
      601 ggaacgctgg cctatcgaga cggcctgtgg agtgggtgcc tggcggaggt attgcaggcg
      661 caggaacctg gagccggcat caccagggat gacgtaagac ctctgctgaa gaacggtttc
      721 ccgtggcaca cccccgacag agcacacccg gagctggaca cacccgaagc gtggtgggaa
      781 gtggtcgagg gactcctgat tctggcatgt ctagaactgg gcttccctga aatgaaggcg
      841 cgtcgatacg cgcgggaagc gcacgaacgg tacatagatg tcgccggctt ccggctcttc
      901 gacgacacat tgtccgtctt gacgaagctc aaggaggaag gttggcgtca cgtcattttg
      961 tcgaaccatg tgccggagtt gcgggacatc gtcgagggcc ttcgactgtc cgtattggtc
     1021 gaggatgtac tgagttccgc ggtgaccgga tacgagaagc cgaatccgga ggcgtttcgg
     1081 ctcggtcgcg aggccgccgg taaccccgat cgactctgga tggtcggcga taacccccaa
     1141 gccgacgtcg ctggggccga agcgataggg atcccggcga tcctcgttcg cagcaaggcc
     1201 gaaggtgttg ctcgcgttgc gacggatctc cacgaggttt tcgagttgat cacccgtatg
     1261 agatgaactc cagaaggttc ccgtcggggt cccggaaata gacgctcgat ccctgcccgc
     1321 gacctccctc tcgctcgacc ggcccctcct cgacggaaac cccatgcgac tccagatgag
     1381 cggtcgcatc ttcgatgggg ccggcccacg tgaagcagac gtgggcgccc ccggcttgca
     1441 cgtccactcg cggccgtagg ggagagtcgg gcacgccggg gccgtggacg ctcaactgtt
     1501 ggtcgccgat ccggtacagc caacggccat ccggttgttg gagcgcttcg actccgacaa
     1561 cgtctcgata gaacgcgttc gagcgctccc aatcggagac ggcgataaca gaatggtcca
     1621 gacgatcgag acccatcacg tcgatcgtag tgcgctggta gttttcatcg ttcttgcttc
     1681 cacaccaagg agcgcaaccg tgaccaacgt gccgggggta ttcaccgcgg agcagcagga
     1741 gctccgctcg atggcgagga aatttctcga agagacctca tcttcgtcgc aggtcagatc
     1801 gctcatcgag accgcagaag gtttcgacga aggggtctgg aaacagatgg ccgagctcgg
     1861 gtggcagggg atcgcgatcc ccgaggagta cggcggctcc ggctacacgt tcaccgaact
     1921 gggagtccta ctcgaggaga tgggccgagc actgctcccc gctcccttct tctcatcggt
     1981 ggttctcggt gccaacgcca tcctcaacgt agcgaccgac gcgcagaaga aggacatcct
     2041 tcccgggatc gccgatggat cggctcgccc cacacttgca ttcgttgagg acgcgggtcg
     2101 ctgggaccag gccgggatcg ggcttgaggc cagcgagagc aagggtggct atcagctgac
     2161 gggacacaag aactacgtca tcgatggtca gacggcgacg catctgatcg tgaccgcacg
     2221 tttggacggc aaggttgcgt tcttcctcgt cgatcccgcc gcagatggag tgactaaaac
     2281 ttcgctcgag actctcgacc ccacgcgcaa gcaggctcgg atcgcgttcg aaggcacacc
     2341 ggcaaccttg ttgggtgatg ccggggccga agacgcgctg accaagaccc tcgatcaagc
     2401 tgcagtgggg ctggcagccg agtccgtcgg cggcgcgcag tggtgcctcg acacggccac
     2461 ggagcacgcc aagacgcgcg tccagttcgg gagaccgatc ggttcgttcc aggccgtcaa
     2521 gcacaggtgt gccgagatgc tcatcgagat cgagatggcc aagtccgccg cctactacgc
     2581 ggcctgggcc gcggccgagg acgagcagga gctcccggtg gcagggtcgc tcgccaaggc
     2641 ctactgctcc gaggcgttct tccatgccgc cgggacgaac atccaggtgc tcggtgggat
     2701 cggcttcacg tgggagcacg acgcgcacct ctacttccgt cgcgctaagt cgtccgagat
     2761 ctacctcggc gacgccgact atcaccgaga actcatcgca gacagaacgg gggtgacggc
     2821 atgaggtggc ggatcgaaga cacgccggag caggccgagt tccgcaagga gttcctcgcc
     2881 tgggttacca agaacatccc ggccggttgg gccgaagcgc tcgaagccgg tgacgaggag
     2941 aagtttgcca aggcgtactc agagttcaac gtcatggagt ggaacagcgt catcggcgga
     3001 gccggttacg cggcccccct gtggccgaag gagtacggcg gcttgtcggg cgaggtgtgg
     3061 atgcagaacg tggtgcgcga ggagcttgct cgactgcggc ttccgctgtt cggcatcaac
     3121 atccttggga tcgggctcgc gggtcccact ctgatccagc acgcgtccga tgaacagaag
     3181 aagcgctacc tccccaagat cctcaccagc gatgaggtgt ggtgccagct cttctcggaa
     3241 ccgggcgcgg gctcggacct cgcgtcgctg tcgacacgag ccgagaagga cggcgacgag
     3301 tgggtcgtca agggccagaa ggtatggacg tcgatcgccg agtgggcgaa gttcgggatg
     3361 ctcgtcgccc ggaccgaccc gagcctgccc aagcacgagg gcctcaccta cttcatctgc
     3421 ccgatgaagg acgacaacgt cgaggttcgt ccgctcaagc agatcaccgg cgcgagcgag
     3481 ttcaacgagg tctacctcaa cgaggttcgc atcccagatt cgcagcgtgt cggcgaggtg
     3541 ggccagggtt gggagatcgc caggaccaca ctcatgaacg agcgggtcgc cctctccggt
     3601 ctgtcgacct cacccgagtc attcctgggt ggtgcacgca aggatccatg gaagacgttc
     3661 gtcgagagcg ttccgaacaa gagcgacacg cttgttcgac agaacgtcgc tcgtttcttc
     3721 atcgagcgcg aagcgaaaga gatgacgtcg ttccgagccg cggccgcccg ccgtatgggc
     3781 gagcagccgg gccccgaagg ggcggccggc aaggtcttca atgccgacat gaaccagcgt
     3841 cagacgtcgt tcgcgatgaa cgcagccggt ccggccgcga tcgcatggca tccggacgac
     3901 aagctcgcgg atggtcgagc gagagctttc ttgagagcgc gcggctactc gatcgaaggc
     3961 ggcaccactg agatcttgaa gaaccaactc gcggaacgca tcctcggcct cccgcgcgat
     4021 cctcaggccg acaagggcgt tccctggaag gacatcaagc gcagctagca gcagcagagg
     4081 aagaaccggc actcgatagg tcccgcctaa cgcgcctgtt cgccgcgacc ttctgtcatt
     4141 tcgtcagcat gggcatgtac atcgcggcgg tgcccctatt cgtgcacttc gagttgggag
     4201 cgtcgcgagc catagtcggg ctggcggtgg gttcgttctt ccccggtgcg atcctgatgc
     4261 gtcccatcat cggggccggc ttggacagtt gggggcgtcg gcccttcgta ttggttgcca
     4321 cggggatcgc cacgctcacg tccctgttgt tcctgttcgc cgacagcgtt tggttcgtga
     4381 tcatgctggg tctgctgcgc gggttcgtgg gggccgcgta ctacacgggg gcagcaacga
     4441 tcgcgaccga catcggcccc gagagccggc gtgcggaggt catcgcgctg ttcagcttgt
     4501 tcctgtacgg aggcttcgcg atcggtcccg ggatcggtga atggctggcc gagaacgtga
     4561 gcttctccgt cgtgtggatg acggctgcgg ggctctccgc cctcgcgttc gcgttcgcga
     4621 ccgggctgcc ggagacactc gaactcaccg aagaggaaga gcggcccccg ggtgggagat
     4681 tcggtctaca ccgagccggt ctggcaccgg ggctggtgct tgtgggagcg gcctccggct
     4741 acaccgcgat caccgggttc gcgccgctct acgcccgcga gatcgggctc gcaacctcgg
     4801 gcggcctcta cgcgacgttc tctctgacga tcctcggttt ccggttggtg actcggaagc
     4861 tggccgatag gcacggtcga ctacagatcg ccctgcccgg attgttcatg aacacgctcg
     4921 gtctcgcgac cctcgcgttg ttcaggtcgg ccttcgccgc ttacctcgcc gtcgccatct
     4981 tcgggatcgg tttcgcgctg ctgttcccgg cactgatggc attcacggcc gaccgtgtcg
     5041 ccgagtctca gcgcggctcc gcgatgacga cgttcacgat gttcttcgac ttcggcgcgt
     5101 tgatcggcac ctatgtcgtc ggattgatcg ccgacaacct cgggttcggg gcagggttcg
     5161 gtttgccggc cgcgatgtgc gcgctcgcga ccctcgggat cggagcgcgt gtgttgttcg
     5221 gtttcggtag acgtcaggcg aagactaggt agagaacctc gccgaccatc gcgggcttct
     5281 cttggccctc gatctcgatc tcgaccccca ccttgtagag gagcccgtca cccttgggct
     5341 gagcttcggt gagtttggct ttgagccgga cgttggagcc ggacggaacc ggattcgtga
     5401 agcgcacctt gtcggttccg tagttgatac ccatcttggc tccg
//
LOCUS       DATMED010000018         3869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5003130, whole genome shotgun sequence.
ACCESSION   DATMED010000018 DATMED010000000
VERSION     DATMED010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5003130"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="VNP73_00410"
     CDS             <1..1752
                     /locus_tag="VNP73_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWL64412.1"
                     /translation="HTIENVTVTNPDDGQTIAITVFKPATAAPEAQVPVIFQSHGWAG
                     TRETDYTGDGAQEDIRNFLDAGFGVVSIDQRGHGESGGLAYTQDPTRETEDIKAVIDY
                     TATLDWVRLDGPGDPVLGAIGGSYGGGYQTMTALDEIADEGRTRFNALAPEITWFDLP
                     QSLAPQKVSRTLWNNLLFAVGAQMLPDYVHTGFAWGTATGQWPDGTMYGQPAPGIVPD
                     LDTVFHRNSPVSFVEEGHQIDVPVLIRQGITDSLFPMNEGLNNFNITVSDEAREQSYF
                     IGYHGGHVLPQVWPQGTAEGDDPCSPNGFNALTIDFFSRVFNGASTDGLLPNRYNFTN
                     VDGETCIGADSIDESRALTVTGLAADQTVTPSGNGAPLQIPVAQGPITVTGVPELSGR
                     ITSAGLDSRMFFGMSMGTSPQDALLIQNNVMPYREIRAVVDKPFALDLAGIAIEVPEG
                     QTLYLTITPTADAFAANGTRGGGAMAFSGLSLSLPQPATIVEQLLSQLRLTIEGKGSK
                     TQLVATLTDSGSAQGIGGAAIDVSGDGVSLGSLVTDETGVARIALPARYRGANHHFEA
                     LFGGNEQYAGSSGTADS"
     gene            1755..2240
                     /locus_tag="VNP73_00415"
     CDS             1755..2240
                     /locus_tag="VNP73_00415"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWL64413.1"
                     /translation="MDPRTEQLDLPKEYGEPKKQLDWADVRRDLEDATVYWVASTRPD
                     GRPHVVPRDGVWLDDSWYYGGSPKTVHNHNLETNPAIAMHIGDGMKAIMVEGESHFVK
                     PPQEIAEQLAEISNRKYAHYGMNNTPETYTERGIWALKARRVIAWNVLFEDATRFVFP
                     E"
     gene            complement(2242..3066)
                     /locus_tag="VNP73_00420"
     CDS             complement(2242..3066)
                     /locus_tag="VNP73_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64414.1"
                     /translation="MLKGTGPSAGLVILSILLLLGAGLYLVLRDDVARLIDRAQSIGD
                     IARPEVEGFTPAYAGVRQLTGDLKAGGMECTDVTVDHEDQYSSIGSCQSSGLHVQINI
                     FLQSSSVQGVEGQLDDDWPFTTVHADNWFVMTETKVARKVHKILGGELFVAADDPFEG
                     TDGGDTGPPSDPRVKRGYKGVRAIIRDINAGGVRCTARTVDQKDDYTSTGSCQVGGTH
                     VQINVYFFGPSIDSVEKSFKDGPFSFVHDDNWFVITQTPVARQIRGALDGRLFVRR"
     gene            3243..>3869
                     /locus_tag="VNP73_00425"
     CDS             3243..>3869
                     /locus_tag="VNP73_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64415.1"
                     /translation="MTTDSTQQHPVADLNSRDNLNDLLDELIEKPLEFDRIKDRIEDV
                     FMQERTIMILDLSGFTRTTQIEGILPFILMIYEMQKLSVPVVEENRGILVRADADNLT
                     CILDSVEDAIEAGRTITDRLESANVILPESRDLYASIGIGHGNILNVGNEAIFGNQVN
                     QASKLGEDIAQKGEVLLTEAAYHQIADNVDIKCEEHKINISGVELTYYS"
BASE COUNT          804 a         1217 c         1181 g          667 t
ORIGIN      
        1 cacaccatcg agaacgtcac ggtaacgaac ccagacgacg gtcagacgat cgcgatcacg
       61 gtgttcaaac cggcaaccgc cgcgcccgag gcccaggtcc cggtgatctt tcaatcgcat
      121 gggtgggccg ggacccgcga aaccgactac acgggtgatg gggcccaaga ggacatcagg
      181 aacttcctcg atgcaggttt cggtgtcgtc agcatcgacc agcgtggaca tggggagagc
      241 ggcggactcg cctacaccca ggaccccacc cgggagaccg aagacatcaa ggccgtcatc
      301 gactacacgg cgaccctcga ctgggtccgc ctcgacggcc ccggagatcc cgtgcttggt
      361 gccatcggag gcagctacgg cggcgggtac cagacgatga ctgccctgga tgagatcgcc
      421 gatgaaggac ggacgcgatt caacgcactg gcacccgaga tcacctggtt cgacctccct
      481 caaagcctcg cgcctcagaa ggtttcacga acgctgtgga acaacttgct gttcgcggtc
      541 ggcgcccaga tgcttcccga ctacgtgcac accgggttcg cctggggcac ggcgaccggg
      601 cagtggccgg acggcacgat gtacggccag ccggcccccg ggatcgttcc cgacctcgac
      661 accgtgttcc atcgcaacag ccccgtctct ttcgttgagg agggacacca gatcgatgtc
      721 cccgttctca tccgtcaagg catcaccgac agcctcttcc ccatgaacga gggactcaac
      781 aacttcaaca tcaccgtgag tgacgaggcc cgcgagcaga gctacttcat cggctaccac
      841 ggaggccacg tgctgccgca ggtctggccg caaggaaccg ccgagggtga cgacccatgc
      901 tcccccaacg ggttcaacgc tctgacgatc gacttcttct cgcgagtgtt caacggcgcg
      961 agcaccgacg ggttgctccc caaccgatac aacttcacga atgtcgacgg cgagacatgc
     1021 atcggcgccg actcgatcga cgagtcgcgt gcactgaccg tgacggggct agcagccgac
     1081 cagacggtta caccgagcgg gaacggagcg ccgctgcaga tcccggttgc tcaaggaccg
     1141 ataaccgtca cgggcgttcc cgagttgtcc gggcgcatca cgtcggcggg cctggattcg
     1201 aggatgttct tcggtatgtc gatgggaaca agtccgcagg acgcgctact gatccagaac
     1261 aacgtcatgc cttaccgaga gatacgggca gttgtagaca agcctttcgc ccttgacctc
     1321 gccggcatcg cgatcgaggt gccggagggg caaacgttgt acctgacgat cactccgacg
     1381 gccgatgcgt tcgcggcgaa cggaaccagg ggcggcggag cgatggcttt cagcgggttg
     1441 tcgttatcgc ttccacagcc ggccacgatc gtcgagcaac tcctgtcgca gctgaggctc
     1501 acgatcgagg gcaagggatc gaaaacccag ctcgtcgcaa ccctgaccga ctccggatcg
     1561 gctcaaggca tcggcggcgc ggcgatcgac gtctccgggg atggagtgtc gctggggagt
     1621 ctggtcacgg acgagaccgg cgtcgctcgc atcgctctcc cggcccgata ccggggagcc
     1681 aaccaccact tcgaagcgct cttcggtggt aacgagcagt acgcagggag ctcgggtacg
     1741 gcagactcgt aaaagtggac cccaggaccg agcaactaga tcttcccaag gagtacgggg
     1801 agcccaagaa gcaactcgac tgggcggacg tacggcgaga cctcgaagac gcgaccgtgt
     1861 actgggtggc gtcgactcga cccgacggta ggccgcacgt ggtcccacgc gatggcgtgt
     1921 ggctggacga ttcctggtac tacgggggca gccccaagac ggtccacaat cacaacctcg
     1981 agaccaaccc ggcgatcgcc atgcatatcg gtgacgggat gaaagcgatc atggtcgagg
     2041 gcgaatctca cttcgtgaag ccgcctcaag agatcgccga gcaactggcc gagatatcca
     2101 accgcaagta cgcccactac gggatgaaca acacaccgga gacctacacg gagcgaggca
     2161 tctgggcact caaggcgcgc cgcgtcatcg catggaacgt gctgttcgag gacgcgaccc
     2221 ggttcgtatt tcccgagtag gtcagcgcct gacgaaaagc ctcccgtcca gggcccctct
     2281 tatctgccgg gcaacgggag tttgtgtgat cacgaaccag ttgtcgtcgt ggacgaaact
     2341 gaaggggcca tctttgaacg acttctccac cgagtcgatc gaaggtccga agaagtagac
     2401 gttgatctgt acgtgcgttc ctcccacctg gcaacttccc gttgaggtgt agtcgtcttt
     2461 ctggtcgaca gttcgggctg tgcaacggac gccccccgcg ttgatgtctc tgatgatcgc
     2521 cctgacccct ttgtagccac gcttgacgcg cgggtcgctg ggggggccgg tgtcaccgcc
     2581 gtccgtgcct tcgaagggat cgtcggccgc gacgaagagc tcgccgccaa ggatcttgtg
     2641 aaccttgcgc gcaaccttcg tttcggtcat cacgaaccag ttgtcggcgt gaaccgtcgt
     2701 gaagggccag tcgtcgtcga gctggccctc gacgccttga accgacgagg actggaggaa
     2761 gatgttgatc tggacgtgga ggcccgatga ctggcagctc ccgatcgagc tgtactggtc
     2821 ttcgtgatcg acggtcacgt cagtgcactc catgcccccc gccttcagat cgccggtcag
     2881 ctggcgcacg cccgcgtacg cgggtgtgaa tccctcgact tcggggcgcg ctatgtcgcc
     2941 gatcgactgc gccctatcga tcagtcgtgc cacgtcgtcc ctgaggacga ggtacaaccc
     3001 ggcccccagg agaaggagga tcgagaggat caccaagccc gcgctcggcc cggtcccttt
     3061 gagcacgaac ggactgctgc tggagagggc gttcccacag ttgccacaga ccgtcgcccc
     3121 tacgggcgac tgataaccgc agttgcggca cgtggggcct ggtggctcgg gaggctcttg
     3181 tgggtcctgc tgcatccgcc aatcatggac caagagattg gctcacgcat tagagtgcgc
     3241 ccatgactac ggactcgacg cagcaacatc cggtagccga cctcaactcc cgagacaacc
     3301 tcaacgacct actcgatgag ctgatcgaga agccgctgga gttcgaccgg atcaaggatc
     3361 ggatcgaaga cgtcttcatg caggagcgga cgatcatgat cctcgacctg agtgggttca
     3421 ctcgaacgac tcagatcgaa gggatccttc cgttcatctt gatgatctac gagatgcaga
     3481 agctctccgt ccccgtcgtt gaggagaaca ggggcatcct cgtccgagcc gacgccgaca
     3541 atctgacgtg catcctggat tcggtcgaag atgccatcga agccggccgc acgatcaccg
     3601 atcggctgga gtctgcaaac gtcatccttc ctgagagccg agacctctac gcgtcaatcg
     3661 gcatcggtca cgggaacatc ctgaacgtgg gcaacgaggc gatcttcggg aaccaggtca
     3721 atcaggcgtc gaagcttgga gaggacatcg ctcagaaggg cgaggtactt ctaaccgaag
     3781 ccgcctacca ccagatcgcc gacaacgtcg acatcaagtg cgaagagcac aagataaata
     3841 tctctggggt cgagctcacg tactactcc
//
LOCUS       DATMED010000019         6652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_14219, whole genome shotgun sequence.
ACCESSION   DATMED010000019 DATMED010000000
VERSION     DATMED010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14219"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VNP73_00430"
     CDS             complement(<1..323)
                     /locus_tag="VNP73_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWL64416.1"
                     /translation="MAGLNRDRSDKLWHDATGLMPGGVSSPVRSFNAVNAHPFFVARG
                     NGAYLTDVDGNEYIDFVQSWGALLFGHAHPNIVQAVTEAAAEGTSFGAVCEREVELAR
                     LVVELV"
     gene            complement(311..1288)
                     /gene="hemB"
                     /locus_tag="VNP73_00435"
     CDS             complement(311..1288)
                     /gene="hemB"
                     /locus_tag="VNP73_00435"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWL64417.1"
                     /translation="MPSFPDSRPRRLRRTSGLRRAFAETSLQPSDLLAPLFLKESLDE
                     PVAISSMPGHYQHSLESLTKEARELAERGVAGVILFGIPTSKDDTGSEAWSKSGISQL
                     GLRALKNELGDDFVVVADLCLCEYTSHGHCGVLDGETVANDPTIEAYGRIAVAQADAG
                     ADVVAPSGMMDGQVRAIRSALDSAGHDDLAILAYAAKYASSFYGPFREAAEGAPKFGD
                     RRAYQQDPSNATEALREVRLDLEEGADAVMVKPAIAYLDVLKAVKDEFGCPTAAYNVS
                     GEYSMLMAAAEKGWIDERAATLETLTAIRRAGADLVITYHAKRAAEWLA"
     gene            complement(1325..2317)
                     /locus_tag="VNP73_00440"
     CDS             complement(1325..2317)
                     /locus_tag="VNP73_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWL64418.1"
                     /translation="MTARRDDERGFTLAELLIAIVVIGILGAISHSVFIHQRVKGWRA
                     QVIAATKNLASVQEYLFNTEGVGSYSLNLAELETVGYNYSAGDVAPAVVAAGTQSFCL
                     EVTSQHDPRIVWHYDSSIGRPEEGPADTDCITGALVAGTQIDGGTSGTSGTSGTSGTS
                     DTSGDSGTSGTSGDGSGSGSEENGDDTDGESDDNSDDSSDSNGNESGGNESDNNGNGN
                     NGNGNGNDSESDDADDDSEDCGDEGESDDEGYQGGDHPSGKSRDDDNGSGGNSGSDPD
                     GDENGGADKPGGDGGADQGDQDGNNGNGNDDDFEDDNNGRDRDGKGGKGKGCSD"
     gene            complement(2452..3957)
                     /gene="cobA"
                     /locus_tag="VNP73_00445"
     CDS             complement(2452..3957)
                     /gene="cobA"
                     /locus_tag="VNP73_00445"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWL64419.1"
                     /translation="MTEPHGPVYLVGAGPGDPGLITRRGLELLRSCDVVLYDRLVDPS
                     LLDEAPTHAERISVGKVPGGPRPAQAAIDRLIVEHAVEGKKVVRLKGGDPFVFGRGAD
                     EAQALAAAGIAFEIVPGVSSAVAVPAYAGIPLTHSGLSSSFAVITAHESAARPGAQER
                     WSELARGATTLVLLMGVGALEETCRRLIDAGLTPDEPAAVIERGTTSDQRTIVGSVGS
                     IAQLAIEAGIEPPAVTIVGPVVSLRDAIAWFETRPLIGVSVVVTRPHVQSKDLARALR
                     AAGAGVIVAPTIAIEDPESWAELDRAVAELSKGAYDWVVFSSRNAVERVNGRVWESEG
                     DARALAGTRIVAVGPGTVGALHSAGLRADLVPDDHSGAGIAAALGSGSGRILLPRPSD
                     APDALPGALRDGGWEVNEVEAYRTVIGPGLPHETVVAGDFDAVIFASGSAVRGFVELY
                     GKADSLGSKVIICIGNSTAEAARELGFEVTVTAAEPTAQALVQALVTHRPK"
     gene            complement(3954..4922)
                     /gene="hemC"
                     /locus_tag="VNP73_00450"
     CDS             complement(3954..4922)
                     /gene="hemC"
                     /locus_tag="VNP73_00450"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWL64420.1"
                     /translation="MSQLPDRLVIASRGSKLALRQAHTVADLVTARHPDLHVEIREVS
                     TKGDRDSRPFTEITGKGLFTSEVEREVAEGRADVAVHSAKDLTAELAPGCTIVGVPER
                     VAPHDVVVGGGSGSGMDRLGSLSSGATVGTSSMRRRALLLELRSDLKVVEFRGNIDTR
                     LEKVARGDVDAAILAAAGIDRLGRDDVDVGPLDPERWVPAPAQGALAVEALEERDDLR
                     RLFGALDDAGARAQVEAERAFAARLEGGCSIPLGCLAFPGPAGLIVTGYLGHPDGFQS
                     IRDRISGGMSEAASLGRELAEAILSSGGDELLEEVRALDAPEVAEP"
     gene            complement(4906..6042)
                     /gene="hemA"
                     /locus_tag="VNP73_00455"
     CDS             complement(4906..6042)
                     /gene="hemA"
                     /locus_tag="VNP73_00455"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWL64421.1"
                     /translation="MSIVVVGVNHRTAPIELLERLSISQEKLTKALHQLDNCEHVLEG
                     AILSTCNRVEVYAVVSRFHGGAQDLKNFLSEFCHVPPEDFTDRAYTYHDEGALKHLFR
                     VAAGIDSMVVGESEILGQVRRAFQIAQEEGMVHRVLGAAGRRAQRVGKRARTETAIGR
                     NPVSVSSAAVELARRAFGAGDLSDKSVAIVGAGKMGGLAARALRTAGADRVTVVNRSE
                     ERAEALAKEFGAEALPLDDLPRVLSEADIVISSTTAPQTVIDRSLVEASLTARSKAPL
                     FMVDIAVPRDVDPDVGDLDGVVLRDIDDLKGVVEVNRESRLGEVSAIEEIIASELEHF
                     LAWERSTEMDPRIVGLLARANESEIEEIERLLRGHFGLDDEPAP"
     gene            complement(6063..>6652)
                     /locus_tag="VNP73_00460"
     CDS             complement(6063..>6652)
                     /locus_tag="VNP73_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWL64422.1"
                     /translation="SSEELASLAGVNAAKVRKDLSYLGSYGTRGVGYDIEYLLYQITR
                     ELGLTQDWPVAIVGVGNLGRALASYKGFSERGFRISALFDIEDAVVGKQIDSMAVHHL
                     DDLKTVVNDENITIGIIATPPLAAQDVADHLVDAGIRSILNFAPAVVNVPPDVNVRKV
                     DLSTELQILSFYQQRTQAIATAREANRRLGAQPKP"
BASE COUNT         1110 a         2230 c         2076 g         1236 t
ORIGIN      
        1 acgagttcaa cgaccagtcg ggccaattcc acctcgcgct cgcacaccgc accgaacgag
       61 gttccctccg ccgccgcctc ggtcactgct tgcacgatgt tcgggtgtgc atgaccgaac
      121 agcagcgcgc cccaagactg cacgaaatcg atgtactcgt tgccgtccac atctgtcaga
      181 tacgcaccgt tgcctcgcgc gacgaagaaa ggatgggcgt tgaccgcgtt gaacgagcgg
      241 accggcgagg agacgccacc gggcatcagg cccgttgcgt cgtgccacaa cttgtcggag
      301 cgatctcggt tcaggccagc cactccgcgg ctcgcttggc gtgatacgtg atcacgagat
      361 cggcccccgc ccgccggatc gccgtcagcg tctccagcgt tgcggcgcgt tcgtcgatcc
      421 aacccttctc ggcggcagcc atcagcatcg agtactcgcc gctgacgttg taggcagccg
      481 tggggcagcc gaactcgtcc ttcaccgcct tcaatacgtc gaggtaggcg atcgccggtt
      541 tcaccatgac cgcatcggcg ccttcctcga ggtcgaggcg aacctctctc agagcttcgg
      601 tcgcgttgga agggtcttgc tggtaggcgc gccggtcacc gaacttcgga gccccctcgg
      661 ccgcttcacg aaagggtccg tagaaagagg atgcgtactt ggccgcgtag gccaggatcg
      721 cgaggtcgtc gtgacccgcg gaatcgagag cgctcctgat cgcgcgtacc tgcccgtcca
      781 tcatcccgga gggagccacc acgtcggccc ccgcatcggc ttgagcaact gcgatgcgtc
      841 cgtaagcctc gatcgtcgga tcgtttgcaa cggtctcgcc gtccagaacg ccgcaatggc
      901 cgtgggacgt gtactcgcac agacataggt ccgcaacgac gacgaagtcg tcacccaatt
      961 cgtttttgag tgcccgcagc cctagttggg agatcccgct cttgctccat gcctcgctgc
     1021 cggtgtcgtc cttgctcgta ggaatgccga agaggatcac gccggccacg ccgcgctccg
     1081 ccagctcgcg cgcttccttc gtcagcgatt cgagcgagtg ctgataatgg ccgggcatcg
     1141 aggagatggc gaccggctcg tcgaggctct ctttgaggaa caggggggcc agcagatcgg
     1201 aaggctgcag tgaagtttca gcaaatgccc gccgtaggcc ggaggttctc ctaaggcggc
     1261 gaggcctgct gtccgggaac gatggcatga agcaatggtg ccacccgccg gccgggtccg
     1321 gtgactagtc ggagcagccc ttgcccttgc cgcctttgcc gtcacgatcg cggccgttgt
     1381 tgtcgtcttc gaaatcgtcg tcgttgccgt tgccgttgtt gccgtcctgg tcgccctggt
     1441 cggccccacc atcgccaccg ggcttgtccg cgccgccgtt ctcgtcgccg tcggggtcgg
     1501 atccggagtt gcccccgctg ccgttgtcgt cgtcgcggct cttaccggac ggatggtcgc
     1561 ctccctggta accctcgtcg tcgctctctc cctcgtcgcc acaatcttcg gagtcgtcgt
     1621 cggcatcatc ggactcggaa tcgttgccgt tcccgttgcc gttgttcccg ttcccgttgt
     1681 tgtcgctctc gttgccaccg ctctcgttac cgttgctatc gcttgaatcg tcggagttgt
     1741 catccgactc accgtcggta tcgtcgccgt tttcttcgga cccggatcct gagccatccc
     1801 cactggtgcc actcgtaccg ctatcaccgc tcgtgtcgga tgttccggaa gttccactcg
     1861 tcccggacgt ccccgaggtg cccccgtcta tctgcgtccc ggccaccagc gcgccggtta
     1921 tgcaatcggt gtcggcgggc ccttcctccg gccgaccgat cgaggagtcg tagtgccaca
     1981 cgatcctggg gtcgtgctgc gaagtgacct cgaggcagaa cgattgggtc ccggccgcca
     2041 ccaccgcagg tgcgacgtca ccggccgagt agttgtatcc gacggtctcg agctcggcga
     2101 ggttgaggct gtacgaaccg acgccctccg tgttgaagag gtactcctgc accgaggcca
     2161 ggttcttggt ggccgcgatg acctgggcac gccagccctt tacgcgttgg tggatgaaaa
     2221 ccgagtgtga gatggctccg aggatcccga taaccactat cgcgatcagg agctcggcga
     2281 gggtgaagcc tcgctcgtcg tctcttcttg ccgtcaaccg atccaacagg ccctcgcttc
     2341 tcgcccctgg tgaccggctc cctcgcccgt cagccacccg cccctacctt cgggtgactg
     2401 gctcgggcta actagttatc ggttcctagt cagtttggct ttaatttcta gtcactttgg
     2461 cctatgagtg accagtgcct gcacgagggc ctgggcggtc ggctcggccg ccgtaacggt
     2521 cacctcgaag cccagctcgc gggccgcctc ggccgtcgaa ttgccgatgc aaatgatgac
     2581 tttcgatccg agtgaatccg ctttgccgta gagctcgacg aaccccctca cggcggagcc
     2641 gctggcgaag atcactgcat cgaagtcccc cgccacgacg gtttcgtggg gcaagcccgg
     2701 tccaatgacc gtgcgatagg cctcgacctc attcacctcc cagccgccat ctcgcagggc
     2761 cccgggcagc gcgtcgggag catccgaagg gcgcggcagc aggatccggc cgctacccga
     2821 tcccagagcg gcggcgatcc cggcaccact gtgatcgtcc ggaacgaggt ccgcgcgcag
     2881 accggccgag tgaagtgcac caacggttcc ggggccgacc gcgacgatcc gtgtcccggc
     2941 gagagcacgg gcatctccct ccgactccca cacacgtccg ttcaccctct cgaccgcgtt
     3001 ccgactggaa aagacgaccc agtcgtaggc gcctttgctc aattcggcga cggcgcggtc
     3061 gagctcggcc cacgactctg gatcctcgat cgcgatcgtg ggagcgacta tcaccccggc
     3121 ccccgccgca cggagagccc gggccagatc tttgctctga acgtgggggc gggtaaccac
     3181 aaccgacact ccgatcagcg gtcgcgtctc gaaccatgcg atcgcgtcgc gtagcgacac
     3241 caccggcccg acgatcgtga cggcgggtgg ctcgatgccg gcttcgatcg caagctgcgc
     3301 gatcgatccg accgatccga cgatggtgcg ttgatccgag gtcgtgcctc tttcgatcac
     3361 cgcagcgggc tcgtcgggag tcagtccggc atcgatgagc cgccggcacg tttcctccag
     3421 cgcgccgacc cccatcaaaa gaacgagcgt tgtcgctccg cgggcgagct cggaccatcg
     3481 ttcctgtgcc ccggggcggg cagcgctctc atgcgcggtg atcaccgcga acgatgacga
     3541 caagccgcta tgcgtcaggg ggatacccgc gtacgcgggg acggcgacgg ccgaagagac
     3601 gcccggaacg atctcgaacg cgatgccggc tgctgcaagt gcttgagcct catcggcccc
     3661 gcgcccgaac acaaagggat caccaccctt cagacgcaca accttcttcc cctctaccgc
     3721 gtgctcgacg atcaggcggt cgatggcggc ctgcgccggg cgcggccccc ccggcacctt
     3781 ccccacggag atgcgttcgg catgcgtcgg cgcttcatcc agcaggcttg ggtccacgag
     3841 ccggtcgtag agaacgacgt cgcaggaccg aagtagctcg agcccgcggc gcgtgatcaa
     3901 gccgggatcg ccgggcccgg cccccacgag gtacaccggc ccgtgtggct cggtcacggc
     3961 tcggcaacct cgggcgcgtc gagcgcgcgc acctcttcca gtagttcgtc tcctccggac
     4021 gacaggatcg cctcggcgag ctcgcgcccg agggaggcgg cttcgctcat gccgcccgag
     4081 atgcggtcgc ggatcgattg aaacccgtcg ggatgaccga ggtacccggt gacgatgaga
     4141 ccggcggggc cgggaaatgc taggcaaccg agcgggatcg aacagccccc ttcgagccgc
     4201 gccgcgaagg cccgttcggc ctcgacctga gctcgggcac cggcatcgtc cagtgctccg
     4261 aatagtcggc gcaagtcgtc tcgctcctcc agagcttcga cggccagggc tccctgtgcg
     4321 ggggccggga cccagcgctc ggggtcgagc gggcccacat cgacgtcgtc tctacccaac
     4381 cgatcgatgc ccgccgccgc gaggatcgcg gcgtcgacgt ctccgcgagc gaccttttcg
     4441 aggcgcgtat cgatgttgcc cctgaactcg accactttga gatccgatcg gagttcgaga
     4501 agcagcgctc gtctgcgcat gctcgaggtg cctaccgtcg ctccgctcga caacgaaccg
     4561 agacggtcca tccccgagcc cgatcccccg ccgaccacaa cgtcgtgtgg ggcgacgcgc
     4621 tcggggactc cgacgatcgt gcagccgggt gccagctcag ccgttaagtc ctttgccgaa
     4681 tgcacggcga cgtcggctcg tccttcggcc acctcgcgct cgacttcaga tgtgaacagg
     4741 cctttaccgg tgatctccgt gaagggcctc gagtcacggt cccctttggt agagacctca
     4801 cggatctcga cgtgcagatc gggatgtcga gcagtgacga ggtcggcgac ggtgtgcgct
     4861 tgtcggagtg cgagcttgga gcctcgcgag gcgattacga gccggtcagg gagctggctc
     4921 atcgtcgagc ccgaagtggc cgcgcagtaa tctctcgatc tcttcgatct ccgactcgtt
     4981 cgctcgagcc aggagtccaa ctatccgagg atccatctcg gtcgaacgct cccatgccag
     5041 gaaatgctcg agctctgaag cgatgatctc ttcgatcgca cttacctcgc ccaagcggct
     5101 ctcccgattg acctcgacca ctcccttgag gtcgtcgatg tctcgcagca cgactccgtc
     5161 gagatccccg acgtcgggat cgacgtcacg aggcaccgcg atgtcgacca tgaacaacgg
     5221 agccttggac cgagcggtaa gagacgcctc caccaacgag cgatctatca ccgtctgagg
     5281 agcagttgtc gacgagatga cgatgtcggc ttcgctcaat acgcggggga gatcgtcgag
     5341 cggcagagcc tctgcgccga actccttcgc cagcgcctcg gctcgctcct cactgcggtt
     5401 gacgaccgtg actcggtcgg caccggccgt cctcaaggca cgtgccgcga gcccacccat
     5461 ctttcccgca ccgacaatcg ctacggattt gtctgataga tcgcccgcgc cgaacgcgcg
     5521 gcgagcgagt tcgacggccg ccgacgagac agataccggg ttccgcccga tggccgtttc
     5581 ggtgcgggca cgtttcccga cccgttgtgc cctccgtccg gctgcaccga gtacccgatg
     5641 gaccatgccc tcttcctgtg cgatctggaa ggctcgtctg acctggccga ggatctcgct
     5701 ctcaccgacg accatcgaat cgatcccggc ggcgacgcgg aagaggtgct tcagcgcgcc
     5761 ctcgtcgtgg tacgtgtagg cgcgatcggt gaagtcctcg ggtggaacgt gacagaactc
     5821 ggacaggaag ttcttgaggt cctgggctcc accgtggaaa cgagacacga ccgcatagac
     5881 ctcgacccgg ttgcaggtcg agaggatcgc tccttccaga acgtgctcgc agttgtcgag
     5941 ctggtggaga gccttggtca gtttctcttg ggagatcgac aggcgctcga gcaactcgat
     6001 gggcgccgtt cgatgattga ctccgactac gactatcgac atcgaggcaa agctatccga
     6061 ggttagggct tgggctgcgc cccgagtcgc cggttggctt cgcgagccgt ggcgatcgct
     6121 tgggtgcgct gttggtagaa ggacaggatc tgcagttcgg tcgagaggtc gacctttcgg
     6181 acgttgacgt ccggagggac gttaacgacg gccggggcga agttcagtat cgaacgtatg
     6241 ccggcatcca cgagatggtc ggccacatcc tgggcggcca gaggcggggt cgcgatgatc
     6301 ccgatagtga tgttctcgtc gttaaccacc gtcttcaggt cgtcaaggtg atggacggcc
     6361 atgctgtcga tctgcttgcc gactacggca tcttcgatgt cgaagagggc cgatatccgg
     6421 aaacctcgct cggagaaccc tttgtaggag gccagcgcgc ggcccaggtt tccgaccccg
     6481 acgatcgcga ccggccagtc ttgagtgagg ccgagctcgc gtgtgatctg gtacaggagg
     6541 tattcgatgt cgtaaccgac gccgcgggtc ccgtaggagc caaggtaaga gagatcctta
     6601 cgcaccttgg ccgcattgac accggccagt gaagcgagtt cttcggagga ga
//
LOCUS       DATMED010000020         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3857877, whole genome shotgun sequence.
ACCESSION   DATMED010000020 DATMED010000000
VERSION     DATMED010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3857877"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VNP73_00465"
     CDS             complement(<1..667)
                     /locus_tag="VNP73_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWL64423.1"
                     /translation="MKGNPTTLGRWLDPPKRYGARIVLFALAVVLLLGPLGLLVTQLT
                     TTDPGLNDLDISAAEAFHPLVTDSPGLVVGLEVISFIGSPPWFYFLLGGASIFLWIKH
                     RRRIAIFIVVTGLMGGLVDTAVKYWVERPRPEIENPVATAPANAFPSGHTMMSTIGYG
                     VLLLVFLPLVPRRWRIPVLLSVLMLVWGIGLARLMLGVHFLTDVVGGFVLGIAWLIAA
                     TVAF"
     gene            complement(685..1650)
                     /locus_tag="VNP73_00470"
     CDS             complement(685..1650)
                     /locus_tag="VNP73_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWL64424.1"
                     /translation="MLRDVGRKRLLIDSFIRFRTADGFSHVRALAHATVLTALPALIT
                     TIAIATAFDFTTFREVLEHTLKQLAPGPTGRLLNTAFRQGSEAGGSAALVGGLIAAVA
                     SGMFAMAQVERGCNRIYGMVRDRSTSAKLTVGLLLSFSAGVLLVLAFVFLAAGQALGK
                     GLSEGGVLGDTASTAFAIARWPVGLLLVFAALTLVYKVSPNRRQPGIAWLQTGTIMAT
                     GLWVILTALLAVYYSVSDQLGDTYGPLLGIIAILTWAYATALALYLGVALAAQLEAVR
                     AGVPGPRTFRRYNETVRDPDETSDSGRFLIPERGQQSRTETAPSL"
     gene            complement(1739..2383)
                     /locus_tag="VNP73_00475"
     CDS             complement(1739..2383)
                     /locus_tag="VNP73_00475"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWL64425.1"
                     /translation="MIKTILDVLGPLYSGALGYLTIAGLIFLDRGAFTGVVLPGELFL
                     ALGGVYAGRGDLSVVAVMLVGALAGLLGETVSFWLGRHYGVRITRHLPLANRFEKHLD
                     EANDYFRRNGGKTIFIGRYVSVVGTFMPFVAGMSKMPFRRFLAFDAGAIALWSIGVTL
                     LGYLLSSQIQLVDRLLSQFGWGLLALVVLFLCGRMIWTRRDEIRKRTAKWRPSS"
     gene            complement(2490..3008)
                     /locus_tag="VNP73_00480"
     CDS             complement(2490..3008)
                     /locus_tag="VNP73_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL64426.1"
                     /translation="MAHALTPTLETPRLRLRKYTYEDLDDLAAMFADAELMRYYSHPK
                     SREESLAWIEWNLGLYEQHGFGLWVMESLEAFEFVGDCGLTPQTVDGVTDIEVGWHTK
                     REYWNQGLATEGASACRDHAFDDLGLQRLISIIHPDNRASRRVAEKIGMTLERTVLHG
                     SGPKVIYAIVRA"
BASE COUNT          649 a          921 c          924 g          523 t
ORIGIN      
        1 tgaacgcaac cgtggccgcg attaaccagg cgatcccgag cacgaagcca cctacaacgt
       61 cggtgaggaa gtgcacccca agcatgaggc gggcgaggcc aatgccccag accaacatca
      121 atacactcag cagcacgggt attcgccacc tgcgcggaac aagtggcaag aagacgagca
      181 acaagactcc gtagccgatc gtggacatca tcgtgtgtcc ggatggaaac gcgttggccg
      241 gtgccgtagc caccgggttc tcgatttcgg ggcgaggccg ttcaacccaa tacttcacag
      301 cggtgtcgac gaggcctccc atcagtcccg tcaccacgat gaagatcgcg atgcgacgac
      361 gatgcttgat ccataggaag atggacgcac caccgagcag gaaatagaac catggcggac
      421 tgccgatgaa gctgatcact tcaagtccga ctacaagccc gggggagtcg gtgaccagcg
      481 ggtgaaacgc ctccgcggcc gagatgtcca ggtcgttgag gccagggtcc gtcgtcgtga
      541 gctgggtgac caggagcccg agggggccga gcagcaagac gaccgcgagc gcgaacagga
      601 cgatccttgc gccgtaacgc ttgggaggat ccagccagcg cccgagcgtc gttgggttac
      661 ccttcatccc tctagcccta tgacctatag ggacggcgcg gtctcggtcc gagactgctg
      721 ccctctctcg ggtatcagga atcttcccga atcactcgtt tcgtccgggt cacgaacggt
      781 ttcgttgtaa cgccgaaacg tgcgcggacc cggaacaccg gcgcggacag cctcgagctg
      841 cgcagccaag gcaaccccga gatacagcgc aagggcggtt gcgtaggccc acgtcaggat
      901 cgcgattatg ccgagcaaag gcccgtaggt gtccccgagt tgatcgctga cggagtagta
      961 cacggcgagc aacgcggtca ggatcaccca tagcccagtg gccataatcg ttccggtctg
     1021 aagccatgcg atcccgggct ggcggcggtt cggcgaaacc ttgtatacga gtgtcagcgc
     1081 ggcgaacacc agcagcaggc ccaccggcca ccgcgcgatc gcgaatgctg tggacgcggt
     1141 gtcacccagt acaccgcctt cggacaggcc tttcccgagg gcttgaccag cagcgaggaa
     1201 cacgaacgcc agaacgagca gcacacccgc actgaagctc aaaaggagcc ccacggtcag
     1261 ctttgctgat gtggaacgat ctcgaaccat cccgtagatc cggttacagc cgcgctcgac
     1321 ctgtgccata gcgaacatcc cagacgccac ggcggcaatc aatccgccaa cgagcgcggc
     1381 actcccgccg gcctccgagc cttggcggaa agccgtgttc aggagccttc ccgtgggccc
     1441 gggggcgagt tgcttcagag tgtgttccag tacctcgcga aacgtcgtga agtcgaaagc
     1501 cgtagcgatg gcgatggtcg tgatgagcgc gggcaacgcg gtcaagactg tcgcatgcgc
     1561 cagggcacga acgtgggaga agccgtcggc cgtccgaaac cggatgaagg aatcgatcaa
     1621 cagtcgcttc cgaccaacat ctcgcagcat tctccgtgcg tcgtcaccat cgagctcgta
     1681 tgtttcgggc acagcactcg acgttgtcaa tcttctcctc ctcgcgtgta ccgtcgcatc
     1741 acgacgacgg tctccacttc gccgtcctct tacggatttc gtcacgccgc gtccagatca
     1801 tccgaccgca caggaacaaa acgacgagcg cgagcaggcc ccacccgaac tgggataaga
     1861 gccggtcgac gagttggatc tgggaactca agaggtagcc gagcagagtc acaccgatgg
     1921 accacaaggc gatcgcgccc gcgtcgaaag cgaggaagcg acggaacggc attttgctca
     1981 tccctgcgac gaagggcatg aaggtcccga ccacggagac atagcggccg atgaatatcg
     2041 tcttgccgcc gttgcggcgg aagtagtcat tcgcctcatc gagatgcttc tcgaaccggt
     2101 ttgcgagtgg cagatgacgc gtgatccgaa ctccgtaatg gcgccccagc cagaagctga
     2161 cggtttcacc aaggagaccc gccagggctc cgacgagcat caccgcgacg actgagagat
     2221 cgccgcgtcc cgcatacacg ccaccgagag cgaggaaaag ctctccaggg agaacgaccc
     2281 ccgtgaatgc tccccgatcg aggaagatca ggccggcgat ggtgaggtat ccgagggcgc
     2341 ccgagtacag gggcccgagg acgtcaagga tggtcttgat cacccaaagt gtttgccctc
     2401 agttggcgaa gcaaaacgag aggtctcgtt tgtcccgatc ggggggcaag caggacatgt
     2461 tgctgatgat cggagcatct tgcgacggat catgcccgta cgatcgcgta gatcaccttg
     2521 gggcctgagc cgtggagaac ggtcctctcg agtgtcatgc cgattttctc tgccaccctg
     2581 cgggatgcgc ggttgtcggg gtggatgatg ctgatcaatc gctgaagtcc gagatcgtcg
     2641 aacgcatggt cccggcacgc gctggccccc tctgttgcga gcccctggtt ccagtactcg
     2701 cgttttgtgt gccagcccac ctcgatgtcg gttactccgt cgacggtctg gggggtgagc
     2761 ccacagtcgc ctacgaactc gaaggcttcc aaggactcca tgacccatag gccgaagccg
     2821 tgctgttcgt aaaggccgag gttccattca atccacgcta aggattcctc tcgtgacttt
     2881 ggatggctgt agtagcgcat gagttcagcg tcggcgaaca tcgctgcgag atcgtcgaga
     2941 tcttcatagg tgtacttccg aagtctcaat cgtggtgttt caagtgtcgg cgtcaaggcg
     3001 tgggccaccg caatcgc
//
LOCUS       DATMED010000021         3212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1167773, whole genome shotgun sequence.
ACCESSION   DATMED010000021 DATMED010000000
VERSION     DATMED010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167773"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VNP73_00485"
     CDS             complement(<1..225)
                     /locus_tag="VNP73_00485"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HWL64427.1"
                     /translation="MTVPLSEREQQILHEMEKNLVAEDPNLADLGGREGGSRGKLGVL
                     AFLAGIVLLFIFFVSQLWFVGLAAFGAMVAG"
     gene            complement(346..1515)
                     /gene="dinB"
                     /locus_tag="VNP73_00490"
     CDS             complement(346..1515)
                     /gene="dinB"
                     /locus_tag="VNP73_00490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWL64428.1"
                     /translation="MQATPILHVDMDAFYASVASLKDPSLKGRPVVVGGQGGRGVVTS
                     ASYEAREFGVHSAMPMVRARRLCPHAAFVPNDFDAYLDYSKKIREVFLSVTPLVEPLS
                     LDEAFLDVGGSRRLFGGPEEIAVRIKHEVANLGLTCTVGVAPNKFLAKLASAAGKPDG
                     LLVVPPDRIFEFLHPLPIGALWGVGPATGEALRKLGLKTVADVAALPRRTLEKAVGES
                     LGAHLHHLANGRDDRSVVTHEPAKSVGNEETFSHDLDSSEEILTEILRLSDRTAERLR
                     SHGLCGRTVTIKVRYANFSTITRSKTLGFEIDTAPEIFEVAGDLFMRLDRVGQRIRLI
                     GVSLAGLAPGPPRRQLGLLEGMPRWDEASKAVDSIRHRFGRGAVRPARLLDHPTD"
     gene            1569..2612
                     /locus_tag="VNP73_00495"
     CDS             1569..2612
                     /locus_tag="VNP73_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWL64429.1"
                     /translation="MEFRTLGKTGVRVSQYCLGAMMFGSWGNSDHDDCVRIIHKALDS
                     DINFVDTADIYGWGESEEIVAKALKGKRDNVVLATKVHGPMGKGQNERGNSRLWIMQE
                     VENSLRRLGVDHIDLYQIHRPEPDTDIEETLGALTDLIHQGKIRYAGCSTFPSWQIVE
                     SHWAAEKRGLSRFVCEQPPYSIFARHIETDVLPVVARYGMGAIVWSPLAGGWLAGKYR
                     RGQDVPEDSRATRAKNFGGRIKDRFDMSRPANQRKLDLVEDLSVVADKAGVSLAHMSL
                     AFALNHPAVTSAIIGPRTMEQLDDVLAGAETRLSDEILDAIDEIVPPGAVIENADAGW
                     DPPWMEPAARRRS"
     gene            2652..>3212
                     /locus_tag="VNP73_00500"
     CDS             2652..>3212
                     /locus_tag="VNP73_00500"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HWL64430.1"
                     /translation="MGPLEEKLTAWVDAGIITGEQANAIRAAESAVPRRRISFVAEIL
                     GYIGAILATVALLMLMGRVWDDFTPGMQVGALLGSSAAMVAAGAALRGNPEPTIDRLA
                     GFLWLASVATFSGGIYLAGVEFLGIDDERPMGLGAGLATTIFATPLWRLRRKGLQQIA
                     VFAPAATTVWFVLPESVDYDTPGWLLA"
BASE COUNT          587 a         1052 c         1044 g          529 t
ORIGIN      
        1 gccggcgacc atggctccga aggcggccag ccccacgaac cacaactggc tcacgaagaa
       61 gatgaacaac aacacgatcc cggccaggaa ggccaggaca ccaagtttgc cgcgcgagcc
      121 gccctcccgg ccgcctaggt ccgcgagatt cggatcctcg gcgacgaggt tcttctccat
      181 ctcgtggagg atctgctgct ctcgctccga tagaggcacg gtcactcgat tatagaaacg
      241 ccactcgcct atttgttcgt tcccgacggc cgagcccgtc gcagttcgct ctttccgacg
      301 gccgagcccg tcgcagttcg ctcttcccga cggccgagcc cgtcgctagt cggtgggatg
      361 gtccaacaat cgtgccggtc ggaccgcgcc tcgaccgaac cgatgacgga tcgagtcgac
      421 ggctttggag gcctcgtccc agcgcggcat cccctccagc aaccccaatt ggcgacgagg
      481 aggccccggt gcaagcccgg ccaagctgac cccgatcaac ctgatccgct gccccaccct
      541 gtccaatcgc atgaatagat cccccgccac ctcgaagatc tcgggggccg tgtcgatctc
      601 gaagccgagg gtcttcgagc gggtgatcgt ggagaagttg gcgtagcgga ccttgatcgt
      661 cacggtgcgc ccacacagcc catgtgaacg aagcctctcg gccgtccgat cggacagacg
      721 cagtatctcg gtgaggatct cttcggaaga gtcgaggtcg tgggagaagg tctcttcgtt
      781 cccgacggac ttggccggtt catgagtcac gaccgagcgg tcgtcgcgtc cgttcgccag
      841 atggtgcaag tgggcaccca gagactcccc caccgccttc tccagggtgc gccggggaag
      901 ggcagccacg tcggcgaccg tcttcaaccc gagcttgcgc agcgcctctc cggtcgccgg
      961 gccgaccccc cacagagcgc cgatcggcag ggggtgaagg aactcgaaga tccggtcggg
     1021 aggaacgacg agaagcccgt cgggtttgcc ggccgcggac gccagctttg ccaggaactt
     1081 gttcggagca actccgaccg tgcaggtcag acccaggttc gccacctcgt gtttgatccg
     1141 gacggcgatc tcctcaggtc ccccgaacag cctgcgagag ccccccacgt cgaggaaggc
     1201 ctcgtccagg gacaagggct cgaccagagg ggtgaccgaa aggaagacct ccctgatctt
     1261 cttcgagtag tcgagatagg catcgaagtc gttcggcacg aaggccgcgt gggggcagag
     1321 ccggcgcgcc ctgaccatcg gcatcgcaga gtgaacgccg aactcccggg cctcgtatga
     1381 ggccgaggtg accactcccc ggcctccctg gcctccgacg accaccggcc ggcccttgag
     1441 gctcgggtcc ttcagcgatg ccacggacgc gtagaaggcg tccatatcga cgtgcagtat
     1501 cggggtggct tgcatctgtt tagggtgggt ccactctaat tagctcgcgc ggcgaaacgg
     1561 aggatcccat ggaattcagg actctcggca agaccggcgt cagggtcagc cagtattgcc
     1621 tcggcgccat gatgttcggt tcgtggggca acagcgacca cgacgattgc gtccgcatca
     1681 tccacaaggc tctcgattcg gacatcaact tcgtggacac ggccgacatc tacggctggg
     1741 gcgaatccga agagatcgtc gcgaaggcac tgaagggcaa gagggacaac gtcgttctcg
     1801 cgacaaaggt ccacgggccg atgggcaagg ggcagaacga acgcggcaac tcacgactgt
     1861 ggatcatgca ggaggtcgag aacagcctgc gcaggctcgg cgtcgaccac atcgacctgt
     1921 accagatcca ccgccccgag cccgacaccg acatcgagga aacgctcggt gcgcttaccg
     1981 atctgatcca ccaagggaag atccgctacg cgggctgctc gacgttcccg tcgtggcaga
     2041 tcgtcgagtc gcactgggcc gccgagaagc gcggtctgtc ccgcttcgtc tgcgaacagc
     2101 cgccctactc gatcttcgct cgccatatcg agaccgacgt gttgcccgtc gtcgctcgct
     2161 acgggatggg agcgatcgtg tggagcccgt tggccggagg gtggctggcc ggcaagtacc
     2221 gaagagggca ggacgtcccc gaggactcca gagcgacccg agcgaagaac ttcgggggcc
     2281 ggatcaagga ccggttcgac atgtcccgtc cggcgaacca gcgcaagctc gacctggtcg
     2341 aggacctttc ggtcgtggca gataaggccg gcgtctcgct ggcccacatg tcgcttgcgt
     2401 tcgcgctgaa tcatccggcc gtgacctcgg cgatcatcgg gccgcgcacg atggagcagt
     2461 tggacgacgt cctcgcgggg gccgagacgc gcctgagcga cgagatcctg gatgcgatcg
     2521 acgagatcgt gccgccggga gcggtgatcg agaacgccga cgccggctgg gacccaccgt
     2581 ggatggagcc ggccgcgcgc agaaggagct agcccgacag ccgaacgtct cgacgttcgg
     2641 cgagaacaag aatgggcccc ctcgaagaga aactcactgc gtgggtcgac gccgggatca
     2701 tcaccggcga gcaagccaac gcgatccgcg ctgccgaatc ggccgttccg cgccgacgga
     2761 tctcgttcgt cgccgagata ctcggctaca tcggtgcgat cctcgccacc gtcgcgctgc
     2821 tcatgctcat gggccgggtg tgggacgact tcaccccggg catgcaggtt ggcgccctgc
     2881 tcggttcgag tgccgccatg gtcgctgcgg gagcggcgct ccgaggtaat ccggagccga
     2941 cgatcgaccg cctcgccggt ttcctgtggc tcgcgtccgt ggctactttc tctggaggca
     3001 tctatctcgc cggagtcgag tttctgggga tcgacgacga gcggccgatg ggcctggggg
     3061 ccgggctcgc aacgacgatc ttcgccacgc cactgtggcg gctgcgcagg aaggggctcc
     3121 agcagatcgc cgtgttcgca ccggcggcga ccactgtttg gttcgtgctg cccgaaagtg
     3181 ttgattacga cacacccgga tggctgctgg cc
//
LOCUS       DATMED010000022         7394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3086412, whole genome shotgun sequence.
ACCESSION   DATMED010000022 DATMED010000000
VERSION     DATMED010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3086412"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VNP73_00505"
     CDS             complement(<1..936)
                     /locus_tag="VNP73_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HWL64431.1"
                     /translation="MAVLDEPLQIGTCTVRNRLYRAPVLEGAGDGPDAPGIYAEAFEA
                     NSKAGVGLIIQGNSCTTEEGRSAPGMTLVNTREATLRLKEVTDAVHRWNARIFLQIGG
                     AGIYAMEGWHATYAAQRRAPLTAVSPPPIHVKPALKGVPVKVLSTAEVRDLIRTFADS
                     ALWAREAGYDGIQLASSNAKLIHQFLTPYYNRRSDEFGGSLRNRARILEEIANAIRAR
                     AGHDFPLTVKIPIGEDAPPFAPHTSFEEGIELCKMAEAFGYHAITPVGLSVFPHASLC
                     RGADPKDILEVKSIKKKFEEALDNSRLKLTVLRWGY"
     gene            complement(936..2240)
                     /locus_tag="VNP73_00510"
     CDS             complement(936..2240)
                     /locus_tag="VNP73_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWL64432.1"
                     /translation="MSDPAEALTALAGSMGELDLGPEDVDITQPGVAELALFSPMMPK
                     VRANPWPIYHRLREQDPVHFSDFGTCLLTRYDDAVAVLRDKRFSVEHRNYLEDRPLNT
                     LGEGFGPAPSERDMSNVMLFVDPPRHTRLRTLVNKAFTPRVVEKLRGRVQEVVDELLD
                     AVEDKGEMDVIEDFSYPLPVKVICEMLGVPQEDTKNIRVWSRQIAPILDPIVTDEAQA
                     GIEEAESHLQPYWDGLIEDRRNDLRDDLLSELIRAEDEGHRLNHEELRSTCNLILIAG
                     HETTMNLIGNGLLALLRNRDQLEKLQENPGLARSAVEELLRFDSPVHLTARSASEELE
                     VGGSKLPRGQLAIIAIGGVNRDPAYFPDPDRLDIERDPNHHIAFSAGAHYCVGATLAR
                     LEAAIAFPRLLERLPKIDLAVESPEYRETITLRGLAELPVTF"
     gene            complement(2237..3268)
                     /locus_tag="VNP73_00515"
     CDS             complement(2237..3268)
                     /locus_tag="VNP73_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWL64433.1"
                     /translation="MLAARFRSSDRSLFLTEVEVPEPGPLEVVVKVEACGICLSDVHM
                     IDGSFPALIEEVTPGHESAGVIAKTGELVQGWKPGDRVVIMAGRVCMRCENCRTGRMD
                     ACLQPELMGFQYDGAWAEYVVVPYFTLTPVPDAIDLEQACVLVDAVATPYAAITERAQ
                     LRPGESIGLWGIGGLGTHAVQVARLVGAGFIVAVDPLPSARDRALKLGADVALDPLEG
                     DVVAEVRRLTGGRGLDVTLDLVGSNKVLQQGVGCLAPAGRAVMVGISIDPLELGPSIL
                     LAVQRQTIMGHLGYSKKNIDELVTLLSSGRLDLTASVSDVIPLEQVPEGVRRLAEKDG
                     DPVRIVVKP"
     gene            complement(4000..4455)
                     /locus_tag="VNP73_00520"
     CDS             complement(4000..4455)
                     /locus_tag="VNP73_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64434.1"
                     /translation="MSATTVATQAVQSGAGAKLKLLRFVLLSIAIGHLVVGFMFWFRP
                     DLAVEEILAWGPASGWTTILGSYDLSVAFALFLAYRNPLDNLGIVKFVGVLLVLHGLT
                     HLYYTVIGDSPARFYFVVSYLIAGGIALLWLGSNEGLKRDPVATPSLGP"
     gene            complement(4452..5204)
                     /locus_tag="VNP73_00525"
     CDS             complement(4452..5204)
                     /locus_tag="VNP73_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL64435.1"
                     /translation="MSDARGGKALLKLERIDAFYGPIKVLFDVNIEVNDGEIVALLGT
                     NGAGKTTILRVISGILRPTVGDVFFKGESIVGQRPSEIVKKGIVQMPGGRGVFPGMTV
                     YENLEIAGFLYGRDKTTRKHMIEKVFEFFPKLAKRKRQSAGSMSGGEQQMLTLAKSFI
                     MDPDLLLIDELSLGLAPKIVQDLLDIIQRLNAEKVAVVIVDQHVDLALDVSSRAYFIE
                     RGEIRFAGPADDLKGRDDLLRSVFLRGEMAKT"
     gene            complement(5164..5949)
                     /locus_tag="VNP73_00530"
     CDS             complement(5164..5949)
                     /locus_tag="VNP73_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL64436.1"
                     /translation="MSLLRTRDISIHFGGIAALDEVDITVEPGEIVGVLGPNGAGKST
                     LFNVISGFLKPDHGRILFLEEEITHLLPYERVGIGLGRTFQHVKLFRNLTVYENLLVA
                     QHRHLQSGSFSAMLRLPGWKRDETYARERVEKILEVVNLRPWKDQRPGELSYGTTRFL
                     ELASVLSIEPKVLLLDEPASGIQQKEVEALGPLLKQIRDETGVAILLIEHDMNLLLNT
                     SERIYALDFGKVIAEGTPDEVTANDQVLESYLGRTRRKSPAKA"
     gene            complement(5960..7084)
                     /locus_tag="VNP73_00535"
     CDS             complement(5960..7084)
                     /locus_tag="VNP73_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL64437.1"
                     /translation="MTAQTLAEETSVLDAQEDKAVDANPEEPKRRFTRAQLVGAVGLL
                     LFALLPIPFGDFGFFVGQYALIYAVLGLSVVVVTGYAGLISLMPYSFAGIGAVVVGLA
                     VDSWGWPFWLALPLATVATVPISVLVGISSVRLKGLYLAIATLTFSNALGETFFKWDA
                     ATGGEAGRIVERPTIGPISLDGDVNLYIFCLACLGLLLWMVEGLRTSRVGRAMLAVKS
                     NELEAQALGINVYKTKLTAFTLSGLIAGFGGGLLALLLSSVTPTAFQSPLAEATSILL
                     VTLVAIGGMDRAIGPLFGAAALVIQQQVFQGAEFFFAFLGIYAAVVLISFMMFRPGGI
                     VEAIKHLVALTRVRPKLGIAIIVVILVTNIGGAWLFLSLS"
     gene            complement(7081..>7394)
                     /locus_tag="VNP73_00540"
     CDS             complement(7081..>7394)
                     /locus_tag="VNP73_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64438.1"
                     /translation="GLLLVLPHSAGTQGGLITTFDVTPFGTLLIPAFGAALVGGLVHL
                     PMALVGGFIFGLARELLVLAPQPWNNLRSAVAAILIVFLLLLRTERFFASRQELEALE
                     G"
BASE COUNT         1448 a         2262 c         2301 g         1383 t
ORIGIN      
        1 gtatccccat ctcaaaaccg tgagcttcag ccgtgagttg tcgagcgcct cctcgaactt
       61 cttcttgatc gacttcacct cgaggatgtc tttcggatcg gcgccgcgac acagagatgc
      121 atggggaaag acggaaaggc cgaccggcgt tattgcgtga tagccgaacg cctcggccat
      181 cttgcacagc tcgatcccct cttcgaagga agtgtgtgga gcgaacggag gagcgtcctc
      241 accgatcggg atcttgaccg tgagagggaa gtcgtgaccc gcgcgggccc ggatcgcgtt
      301 ggcgatctct tcgaggatgc gggcccgatt gcgaaggctg cccccgaact cgtcgctccg
      361 tcggttgtaa tacggggtga ggaactgatg gatcagcttg gcgttcgagg acgcgagctg
      421 tatcccgtcg tagccggcct cgcgtgccca tagcgcgctg tctgcgaacg tccggatgag
      481 gtcgcggacc tctgcagtcg acaagacctt caccggcact cccttaagag caggcttgac
      541 atggatcggg ggaggcgaca ccgcggttag aggagcacga cgctgtgccg cgtaggtcgc
      601 gtgccacccc tccatcgcat agatgcccgc tccgccgatc tgcaggaaga tccgagcgtt
      661 ccagcgatga acggcgtccg taacctcttt caaccggagc gtcgcctcgc gcgtgttgac
      721 gagtgtcatc cccggcgccg accggccctc ttcggtcgtg caggagttgc cctggatgat
      781 gagaccgacg ccggccttgg agttcgcctc gaacgcttcc gcatagatcc cgggagcgtc
      841 gggaccgtca ccggcaccct cgaggaccgg cgcgcgatag aggcggttgc gaacggtgca
      901 ggttccgatc tggagcggtt cgtcgaggac ggccatcaga aggtgaccgg gagttccgcc
      961 agtcctctga gcgtgatcgt ctctcgatat tcgggtgact ccactgcgag gtcgatcttc
     1021 ggcagacgtt cgagcagacg agggaacgcg atcgcggctt cgagtcgcgc cagcgtggcg
     1081 ccgacgcagt aatgggcccc cgccgagaag gcgatgtggt ggttcgggtc ccgctcgata
     1141 tcgagcctgt ctgggtcggg gaagtaagcc ggatcgcggt tcacgccccc gatcgcgatg
     1201 atcgccagct ggccgcgcgg gagtttcgat ccccccactt caagctcttc cgacgcgctc
     1261 cgtgcggtta ggtgcaccgg cgagtcgaac cgaaggagtt cctcgaccgc gctcctggcc
     1321 aacccggggt tctcctgcaa cttctccaac tgatcacggt tgcggaggag ggccaggaga
     1381 ccgttgccga tgaggttcat cgtcgtctcg tgtccggcga taaggatcaa gttgcaggtc
     1441 gagcgcaatt cctcgtggtt tagacggtga ccttcgtcct ctgcccggat caattcgctg
     1501 aggagatcgt cccgcagatc gttccgtctg tcctcgatca gcccgtccca gtagggctgc
     1561 aggtgggact cggcttcctc gatccctgcc tgggcttcgt cggtcacgat cgggtcgagg
     1621 atcggagcga tctgtcgcga ccacactcgg atgttcttcg tgtcctcttg cggcaccccg
     1681 agcatctcgc agatcacctt gaccgggagc gggtacgaga agtcctcgat gacgtccatc
     1741 tcgcctttgt cttcgaccgc atcgagcagt tcgtcaacga cctcctgcac gcggccgcgc
     1801 aacttctcga cgacccgagg tgtgaacgcc ttgttcacga gggtcctcaa gcgagtgtgt
     1861 cgcggcggat cgacgaacag catcacgttg gacatgtcgc gttcgctggg ggccggaccg
     1921 aacccctcac ccaacgtatt tagggggcgg tcctcgagat agttgcggtg ctcgacagag
     1981 aagcgcttgt cgcgcagaac ggccaccgca tcgtcgtagc gagttagtaa gcaggttcca
     2041 aaatcgctga aatggaccgg gtcctgctcc cttaagcggt ggtagatcgg ccacgggttt
     2101 gctcggacct taggcatcat cggactgaac agcgccaatt cggcgacgcc cggttgggtg
     2161 atgtcgacgt cttcgggtcc gaggtcaagt tcgcccatcg acccggccaa tgcagtgagg
     2221 gcttcggcgg gatcgctcat ggcttcacca cgatccgcac agggtctccg tccttctcgg
     2281 cgagccggcg cacgccttcc ggaacctgct ccaacggaat gacatccgaa accgatgccg
     2341 tgagatccag ccggcccgat gacagcaggg ttactaactc gtcgatgttc ttcttcgaat
     2401 agccgaggtg gcccatgatc gtctgtctct ggacggcaag gaggatgctg ggcccgagct
     2461 cgagtgggtc gatcgagatg ccgaccatga ctgcccgacc ggcaggggcg aggcacccga
     2521 cgccttgttg aagcactttg ttcgacccca cgaggtcaag tgtgacatcg agaccgcgac
     2581 cgccggtcag tctccttact tcggccacga cgtccccctc aagggggtcc agggccacgt
     2641 cggcgccgag tttcagagca cggtcgcggg cactcggcag agggtcgacg gcgacgatga
     2701 atccggctcc aacgagtcgt gcgacctgga ccgcgtgcgt accgagccct ccgatgcccc
     2761 acaagccgat cgattcaccg ggacgaagct gagcacgctc ggtgatcgcc gcgtaaggag
     2821 tcgccaccgc gtcgaccagt acgcaagctt gttcgaggtc gatcgcgtcg ggaacgggag
     2881 tcagtgtgaa gtaggggaca acgacgtact cggcccacgc cccgtcgtac tggaagccca
     2941 tcaactccgg ctgcaagcac gcgtccatgc ggccggtccg gcagttctcg cagcgcatgc
     3001 agacgcgacc ggccatgatc accacgcgat ctcccggctt ccagccctgc acgagttcgc
     3061 ccgtcttggc gatcacgccg gccgattcgt gtcccggcgt tacctcttcg ataagagccg
     3121 ggaacgatcc gtcgatcatg tggacatcgg acagacagat gccgcatgcc tcgaccttga
     3181 ccacgacctc aagggggccg ggctcgggga cctcaacctc cgtaaggaag agtgaccggt
     3241 cggaagagcg gaagcgggcg gccagcatcg gccaaaccta ttcggttgcc actgaagagt
     3301 taagtcagta agggcaactt cgaccgagga ggggcaaggg aagacggcct gatcgaggca
     3361 tcctggtggc gcctctggct gtagccggag gcgctctgtc ttgccagttc ggagcgcgac
     3421 tccagatcct gaggcgctga ggctaaactt gcgactcagg gggaattgat gatggaacgt
     3481 agaagaaccc tcgctctttt ggtgttggtc atggccgtct tggcttcgat cccggtttcc
     3541 gctgcagcac tttcagtgga tcaatctcag gttttggtaa gagagaaagt cacggctcgc
     3601 gagtattcct gtcgatctga ctcgtgccag cgcttcatcg gagagatgaa cctaaggtgt
     3661 ttgcttcagc ctcatgctcg tagtcctaac tctttgggct catgcgccaa ggcatcagct
     3721 ccaggaatgt gtctagtgca cggggggtgt ccccctgcaa gacctaagac ctgtctgatg
     3781 gatgaacggt gccctgatcg aatagcgacg gtctgtttaa tccgcggagg ggagcaagga
     3841 ctatcacggc gcatcgcacg cgatcgcggc tggtcttcgc cgactcaaag acgtgaactt
     3901 tgccagtcgt aggcaccttt atccgaaatc aacgttcgta tgagacgtta caaggtaggc
     3961 cgatcactcc aacgaaagaa agtcagcgat tggtcggaat cacggcccca gtgagggtgt
     4021 ggctaccggg tcgcgcttca ggccttcgtt gctcccgagc cacagcagag cgataccccc
     4081 ggcgatcaga tagctcacaa cgaagtagaa gcgggccgga gagtcgccga tgaccgtgta
     4141 atagagatgg gtcaggccgt gtagaaccag caacacaccg acgaacttca cgatgccgag
     4201 gttgtcgagc gggttccgat aggcgagaaa caacgcgaac gccaccgata ggtcgtagga
     4261 acccaggatc gtggtccatc ccgatgccgg gccccacgca aggatctcct ccaccgccag
     4321 atcgggccgg aaccagaaca tgaaccccac cacgaggtgg ccaatcgcga tggacagaag
     4381 aacgaagcga aggagcttga gcttcgcacc ggcacccgac tgcacggcct gtgttgccac
     4441 ggtggttgca ctcatgtctt cgccatctcc ccgcgaagga acaccgatct caggagatcg
     4501 tcccggccct tcaggtcatc ggccggtcct gcgaagcgga tctctcctcg ctcgatgaag
     4561 taagcgcgtg acgaaacgtc gagagcgagg tcgacgtgtt ggtcgacgat caccaccgcg
     4621 accttctcgg cgttgagccg ctggatgatg tcgaggaggt cctggacgat cttgggagcc
     4681 agcccgagtg atagctcgtc gatcagcaga agatccggat ccatgatgaa tgacttcgcc
     4741 agagtcaaca tctgctgttc gccgcccgac atgctcccgg ccgactggcg tttccgcttc
     4801 gcgagcttcg ggaagaactc gaacaccttc tcgatcatgt gtttccgagt ggtcttgtcc
     4861 cgaccgtaga ggaatcctgc gatctcgaga ttctcgtaaa cggtcatgcc cggaaatacg
     4921 ccgcggcccc cgggcatctg gacgatcccc ttcttgacga tctcggaagg gcgctgaccg
     4981 acgatcgatt cacccttgaa gaagacgtct ccaacagtcg gacggaggat ccccgagatc
     5041 actcgcagga tcgtggtttt gcccgcgccg ttcgtcccga gtagtgcgac gatctctccg
     5101 tcgttcacct cgatgttgac gtcgaacagg acctttatcg ggccgtagaa cgcgtcgatc
     5161 cgctcaagct ttagcagggc ttttcctcct cgtgcgtccg agataggact cgagcacctg
     5221 gtcgtttgcc gtcacttcgt ccggcgttcc ttccgcgatc acttttccga agtcgagcgc
     5281 gtagatgcgt tccgacgtgt tgaggaggag attcatatcg tgctcgatca gaaggatcgc
     5341 gaccccggtt tcatcgcgga tctgtttcag caacggtcct agcgcctcga cctctttctg
     5401 ctggatgccg gaagccggtt cgtctagcag cagcaccttc ggctcgatcg acagcacgct
     5461 ggccagctcg aggaagcgag tcgtgccgta ggacagctcg ccggggcgct gatccttcca
     5521 cggccgcagg ttgacgactt cgaggatctt ctcgacccgt tcgcgcgcgt aggtctcgtc
     5581 gcgcttccat ccggggagtc tcaacatggc cgagaatgat ccgctctgca gatggcggtg
     5641 ttgtgctaca agcaggttct cgtagacggt caggttgcgg aacagcttca cgtgctggaa
     5701 cgtacgaccg agaccgatcc ccactcgctc gtacggaagc aggtgcgtga tctcctcttc
     5761 gagaaagagg atgcggccgt gatccggctt caggaaaccg cttatgacgt tgaacaaggt
     5821 gctcttgccg gctccgttcg gacccagcac gccgacgatc tcaccgggtt cgacggttat
     5881 atcgacctcg tcgagcgcgg cgatgccccc gaagtggatg ctgatgtctc tcgttcgaag
     5941 caggctcatc agataactcc tatgacaggg acaggaagag ccaggctccg ccgatattgg
     6001 tgacgaggat gacgacgatg atcgcgatac cgagcttcgg ccgcacgcgg gtaagcgcga
     6061 cgagatgctt gatcgcttcg acgatgccgc cggggcggaa catcatgaaa gagatcaaca
     6121 ccactgccgc gtagatcccg aggaaggcga agaagaactc ggccccctgg aacacctgct
     6181 gctggatcac gagcgcagct gccccgaaga gggggccgat cgcccggtcc atcccaccga
     6241 tggccaccag ggtcacgagc aggatcgacg ttgcttcggc gagcggagac tggaacgcgg
     6301 tcggcgtcac gctcgacagc aagagggcca gcagcccgcc accgaatccg gcgatcagcc
     6361 cggaaagcgt gaaggccgtc agcttggtct tgtagacgtt gatgccgagg gcctgagcct
     6421 cgagttcgtt gctcttgacc gcgagcatgg cgcgccctac ccgtgacgtc ctcagtccct
     6481 cgaccatcca cagcaacagt ccgagacagg cgaggcagaa gatgtagaga ttcacgtcgc
     6541 cgtcgaggct gatcggcccg atcgtcgggc gctccacgat ccgtcctgcc tcgccgcccg
     6601 tggccgcgtc ccatttgaag aacgtctctc ccagcgcgtt cgagaacgtg agggttgcga
     6661 tggcgagata gagacccttc aaccgaacag aagagatccc gaccagtacc gagatcggaa
     6721 cggttgcgac cgtcgccagt gggagcgcga gccagaatgg ccacccccac gagtcgaccg
     6781 cgaggccgac aacgaccgcc ccgatccctg cgaacgagta cggcatgaga gagatgagac
     6841 ccgcatagcc ggttacgacg accaccgaca gtccgaggac cgcatagatg agcgcgtatt
     6901 gaccgacgaa gaaaccgaag tccccaaagg ggatggggag caatgcgaac aggagaaggc
     6961 cgacggcccc gaccagctga gcccgcgtga acctgcgctt gggttcctct gggttcgcgt
     7021 cgacggcctt gtcttcttgt gcgtcgagga ccgacgtttc ctcggcgagt gtttgtgcgg
     7081 tcatccttcg agcgcctcca actcttggcg agaggcgaag aaccgctcgg tgcggagcaa
     7141 cagcaagaac acgatcagga tcgcggcgac tgcggaccgc aggttgttcc acggctgcgg
     7201 ggcaaggacg agcagctccc gcgccagccc gaatatgaat ccacccacca gcgccatcgg
     7261 aaggtgtacg agtcctccga ccagcgccgc cccgaacgcg gggatcaata gggtcccgaa
     7321 cggggtgacg tcgaacgttg tgatcagccc gccctgagtc ccggccgagt gtggaagtac
     7381 gaggagaaga ccga
//
LOCUS       DATMED010000023         5615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4624510, whole genome shotgun sequence.
ACCESSION   DATMED010000023 DATMED010000000
VERSION     DATMED010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4624510"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VNP73_00545"
     CDS             <1..565
                     /locus_tag="VNP73_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWL64439.1"
                     /translation="DRYLLGEVEELDRVPVTSVPRTLMDVCGIRDHRAERALDHALRT
                     DLTNLGRMWLLYDQEWTRGRRGIALLRGHLVERTPGRAPSDGELADLMWGIVRSRRLP
                     EPIAQHPVKLPSGLVVHLDYAYPEAMLAIETDGYAYHQDRRSFELDRERDAELQLLGW
                     TTLRLTWSKLRFDPDYVERLLRHHLCP"
     gene            706..2142
                     /locus_tag="VNP73_00550"
     CDS             706..2142
                     /locus_tag="VNP73_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64440.1"
                     /translation="MKKALKFVLVTTLFAGLVPLAGAARSAVSCPSVKPPKLGFGQPI
                     WIDKNRAGGEPVSIVAQDGSITVSAHAGTTHVYKNPEAVAGAGDFAGSYYNQTLNWRS
                     EDGGESWQYIGLAGAGTGPHSASSTGFSDPDLTMDAGGKLYNVEIDLANVAVFASPDD
                     GQSWPTANPVAASGDRPWVTGAEADEVFLYVNLPKQLWRSTDGGLTFSLVNQDFPADG
                     KLLVDPLNPKKGLIGPSGSGIAISPDDGQTWKDYPGTLGRSTQFFGAVAVDRAGWAYA
                     ASAGGYSGSTDADANGSVAFNYFDRQKKKWASRPIDIPIPKGDALWPWIIAGDDGRVA
                     VVWYQTHGSDSDEFYIYAAYTTNAHGTTVRCSDGSKRFVPPQFSVANASGRPIHKGKI
                     CLSGTTCNANPNFEGGDRRLGDFFTVNFDHTGRMFIVSADTTLKSPTGGPKPVANPLF
                     IKQSHGDMLLARPDKTRKSRCLFPLQDC"
     gene            2167..2523
                     /locus_tag="VNP73_00555"
     CDS             2167..2523
                     /locus_tag="VNP73_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64441.1"
                     /translation="MRKALVGVVAATALFVPTVSPALGSGSPTTDTAREPESCYAFNP
                     GQPTCSFTVTSTSSSGTVTGAVGPGDWVVVVKRGKQKIVLKPTSPEPVAFAYQVGDKV
                     KATVNAAGGFVLAGHD"
     gene            2623..3621
                     /gene="glpX"
                     /locus_tag="VNP73_00560"
     CDS             2623..3621
                     /gene="glpX"
                     /locus_tag="VNP73_00560"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HWL64442.1"
                     /translation="MAQGSTRETPDRNLAMELVRVTESAALAAARWMGRGDKEAADQA
                     AVDAMRVMLDTVPMDGVVVIGEGEKDNAPMLFNGEQIGDGQPPAVDIAVDPIDGTTLT
                     SKGMNGALSVVALAERGTMFDPGPCVYMEKIATGPEGKDVIDLDAPIGENLERLAKVK
                     DVEVASLTVIMLERERHDQLVKEVREAGAHIKLISDGDVAAAIAAARSQSNVDMLVGI
                     GGTPEGVISAAAIKCLGGVIQGRLWPRNEQEKGEAIAAGYDLDRVLTTDDLVSAENVF
                     FAATGITDGDLVKGVTYRGDDAITQSIVMRSKSGTIRLIDAVHRRKKLAQYSTIEY"
     gene            3662..4951
                     /locus_tag="VNP73_00565"
     CDS             3662..4951
                     /locus_tag="VNP73_00565"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL64443.1"
                     /translation="MRRARSLALDISPITESVSYRALWVGMTVSLIGTNMRMVGVPWL
                     VFELTDSSVAVGLVGLAEVVPLLVFSIFAGAIVDRVERRGLIARMQLSMLATSGLLAV
                     LSFSDRPPLVAVYALTALASSLSAIERPARTAMLPRLVPEGKLAAAFALRQLSFQSTL
                     IVGPALAGLVIASVGVQWVFVIDAVSYVASLFVLRWLPRDRPENNQEQSPFDSLREGL
                     RFTFGTRLIMSIFAIDLIAMIFGMPRAVFPALAKHTFAIGAGGAGLLYAAPAVGAVIG
                     ALTTGWVTRIHRQGMAVVLSVAVWGGAITLAGLSTAWLPVTLVFLAAAGAADAISAVF
                     RGAMLVQETPAQLWGRVSSVNLMVVVGGPLIGDFEAGVVAGVTSPATSVVLGGLVCLA
                     GTLVVALLSPLMRYRKPPTDQAGGRTGGPGEEPSNRT"
     gene            4972..>5615
                     /locus_tag="VNP73_00570"
     CDS             4972..>5615
                     /locus_tag="VNP73_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64444.1"
                     /translation="MKVWRGCVAVSLVLLMAGAAWPAGATPVAKTKAERESLGRSFLE
                     PLESTNFIQLGDEPGEHEFAPAFQLLEKLFPRYAEYTTVDEELNDPNAVSVGPDGFPA
                     WDKKDTGDGLPFDVAYVTDKSVPDHKKGYVLLTVAHSAEPCGREGAIRFLEDLLIWRT
                     SDPDHKLTDQSGLKGEEHKITVEELLKRTKIYFVNVAPDGWTAGDGYGNMYDQS"
BASE COUNT          994 a         1765 c         1830 g         1026 t
ORIGIN      
        1 agatcgctac ctgctcggcg aggtcgaaga gctcgatcgt gtgcctgtta catcggtgcc
       61 caggactctc atggatgtgt gtgggatccg cgatcacagg gcggagcgag ccctcgatca
      121 tgctcttcga actgacttga cgaacctggg ccgcatgtgg ctcctctatg accaggagtg
      181 gacccgtggg agaaggggga tcgcgctcct tcgcgggcac ctggttgaga gaacaccggg
      241 tcgggcacct tccgatgggg agctcgcgga tctgatgtgg ggcatcgttc gaagtcgaag
      301 gcttccagag cccattgccc agcatcccgt gaagcttccg tccggactcg tggtgcatct
      361 ggactacgcc tatccggagg cgatgctcgc gatcgagacg gacggctacg cctaccacca
      421 ggatcgcagg tcgttcgagc tggatcgcga gagggacgcc gagcttcagc tgctcgggtg
      481 gactaccctt cgcctcacct ggtcgaagct gcgcttcgac cccgactacg tcgagaggct
      541 gctccgccac catctatgcc cgtagccccc cctgccggct tgggcgtgac ttaccgaata
      601 tgtaagtgag acccaactcg gcgaggctgg ggatcgggca ggggagcgag ctcgtagggc
      661 gaacctttta gggcagacag ccctatccgg aggtgacccc cggccgtgaa gaaggccctc
      721 aagttcgtcc tggtgacaac tttgttcgcc ggtttggtgc ccctggcggg tgccgctcgc
      781 tcggccgtct cgtgtccctc cgtaaagccg cccaagctcg ggttcggcca gccgatctgg
      841 atcgacaaga accgcgccgg cggggagccg gtcagcatcg tcgcccagga tggctcgata
      901 accgtttcgg ctcatgcggg caccacgcac gtctacaaga accccgaggc cgtggcaggc
      961 gccggcgatt tcgcaggttc ctattacaac cagacgctca actggcgcag tgaagacggc
     1021 ggcgagtcgt ggcaatacat cggcctcgcc ggagcgggta caggtccgca cagtgccagc
     1081 tcgaccggct tctcggaccc cgacctgacg atggatgccg gcggcaagct ctacaacgtc
     1141 gagatcgacc tcgccaacgt ggccgtgttc gcgagcccgg atgacgggca gtcgtggccg
     1201 accgctaacc cggtcgccgc gtcgggagac cgtccctggg ttaccggggc cgaggccgat
     1261 gaggtgttcc tgtacgtgaa cctgccgaag cagttatggc gctcgaccga cggtggcctc
     1321 accttcagcc tcgtgaatca ggactttccc gccgacggaa agcttctcgt cgacccgctc
     1381 aacccgaaga agggtctgat cggtccttcc ggttcgggca tagcgatctc gcccgacgac
     1441 ggacagacgt ggaaggacta ccccggcacc ctcggcagat cgacgcagtt cttcggcgcc
     1501 gtcgcggtgg accgtgcagg ttgggcctac gcggcttcgg ccgggggcta ctccggttcc
     1561 accgacgcgg acgcgaacgg gagcgttgcc ttcaactact ttgaccgcca gaagaagaag
     1621 tgggcgtcgc gtccgatcga tatcccgatc cccaagggag acgctctgtg gccgtggatc
     1681 atcgccgggg acgacggtcg cgttgcggtt gtctggtacc agacccacgg ctctgattca
     1741 gacgagttct atatctacgc cgcctacacc acgaatgccc acggaaccac cgttcgctgc
     1801 tcggacggga gtaagcgatt cgtacccccg cagttctcgg tcgcgaacgc ttccggtcgc
     1861 ccgatccaca agggaaagat ctgtttgagc gggactacct gcaacgcgaa cccgaacttc
     1921 gaaggtgggg acaggcgcct cggcgacttc ttcacggtca acttcgacca caccggtcgg
     1981 atgttcatcg tgtccgccga tacgacattg aagagtccca ccggagggcc taagccggtg
     2041 gccaatccgc tgttcatcaa gcagagccat ggagacatgt tgctagcgcg gcccgataag
     2101 acccgcaaga gtcgctgtct cttcccactg caggactgtt agaggacgaa aggacaaggg
     2161 ggcaccatgc gtaaggcatt agtaggagtg gtagctgcaa cggcgctgtt cgttccgacc
     2221 gtgtcgccgg cgttgggcag cgggtcgcct acgaccgaca cagctcgcga gccggagtcg
     2281 tgctacgcgt tcaatcccgg gcaaccgacg tgcagcttca cagtcacgag cacctcgtct
     2341 agcggtaccg tcaccggagc cgtcggcccc ggcgactggg tcgtcgttgt gaagcggggt
     2401 aagcagaaga tcgtcctgaa gccgacgagt cccgagccgg tagcgttcgc gtaccaggtg
     2461 ggcgacaaag tcaaggcgac ggttaacgct gcagggggct tcgtcctcgc cggacacgac
     2521 tgatccacag agaagtacga accaaacggg cgctgcgaga gcggcgccct tttccttggg
     2581 cgttaacctt gccgtcctcg caacccggac aaggaggtct tgatggctca ggggtccacc
     2641 cgtgagaccc ccgatcgcaa cctggcgatg gaactcgttc gggtgaccga atcggcggcg
     2701 ctggcggctg cgcggtggat gggacgtggc gacaaggagg cggccgatca ggccgcggtc
     2761 gatgcgatgc gtgtgatgct cgacaccgtc cccatggatg gggttgtcgt tatcggcgag
     2821 ggcgagaagg acaacgcgcc gatgctcttc aatggagagc agataggcga cgggcagcca
     2881 cccgctgtgg acatcgctgt ggatccgatc gacggcacca ctcttacctc caagggcatg
     2941 aacggagctc tatcggtcgt ggctctagct gagcggggaa cgatgttcga ccccggtcct
     3001 tgcgtctaca tggagaagat cgccaccggg cccgagggca aagacgtaat cgatctcgac
     3061 gctccgatcg gagagaacct cgaacgcctg gccaaggtca aggacgtcga agtggcgagc
     3121 ctcacggtca tcatgctcga gcgggagcgt cacgatcagc tcgtcaagga ggttcgagag
     3181 gccggagcgc acatcaagtt gatcagtgac ggagacgtcg cggcagccat agctgccgcc
     3241 cgatcgcaat ccaacgtgga catgcttgtg ggtatcggag gaacacccga gggggtgatc
     3301 tcggctgccg ccatcaagtg tctcggtgga gtgatccagg gacgcctatg gccccgcaac
     3361 gaacaggaga agggggaggc gatcgctgcc ggctacgacc tcgaccgtgt cctgaccacc
     3421 gacgatctcg tttctgccga aaacgtgttc ttcgccgcga ccgggatcac ggacggcgac
     3481 ctggtgaagg gcgtgacgta ccgcggggac gatgcgatca cgcaatcgat cgttatgcga
     3541 tcgaagtccg gcacgatccg gctgatcgac gccgtgcatc gtcgcaagaa gctcgcgcag
     3601 tactcgacga tcgagtactg agctgggcga tccctctcgc cccggcgccg cacgccgcgg
     3661 cttgaggcga gcgcgctcgc tcgctctcga catatcgccg atcaccgagt ccgtctctta
     3721 ccgcgctctg tgggtcggga tgaccgtgtc cctgatcggg acgaacatgc gcatggtcgg
     3781 tgtcccgtgg ctggtgttcg agctgaccga ctcgagtgtc gcggtcggcc tggtcggcct
     3841 ggccgaggtc gttcctctgc tcgtgttctc gatctttgcc ggcgccatcg tcgaccgggt
     3901 agagcgcagg gggctgatcg cgcgcatgca gctcagcatg cttgctactt cgggtctcct
     3961 ggcggtgttg tccttctccg accgcccacc gctcgtggcg gtatacgcat tgaccgcgct
     4021 tgcctcgtct ctcagcgcga tcgagcggcc ggcccggacc gcgatgctgc cgcgcctcgt
     4081 tccggaggga aagctggcgg ccgcgttcgc tctccgtcag ctctcgttcc agtcgacgct
     4141 gatcgtgggt cctgcacttg ccggtctcgt gatcgcatcc gtcggagttc agtgggtgtt
     4201 cgtcatcgat gcggtgagtt atgtggcatc gctgtttgtc ctgcgatggc tcccacgcga
     4261 ccggcctgag aacaaccaag agcagtctcc tttcgattcc cttcgcgagg gactgcgctt
     4321 cacgttcggt acgcgcttga tcatgtctat cttcgcgatc gacctcatcg cgatgatctt
     4381 cgggatgccg cgcgccgtct tccctgctct cgccaaacac acgttcgcga tcggtgcagg
     4441 cggcgcgggt ctgctctacg cggcccccgc agtaggtgcc gtgatcggcg cactcacgac
     4501 cggatgggtg acccggatcc atcgtcaggg gatggctgtc gtgctctcgg tcgccgtgtg
     4561 gggcggcgcg atcacacttg cgggactctc aacagcatgg ctgccggtga cactcgtctt
     4621 cctcgccgcc gccggagcgg ccgacgcgat cagcgccgtg ttccgcgggg cgatgctggt
     4681 ccaggagaca cccgcgcagc tctggggccg cgtgtcctcg gtcaatctga tggtggtcgt
     4741 cggtggacct ttgatcggtg acttcgaggc cggggtggtg gcaggcgtga cgagcccggc
     4801 cacgtcggtc gtcctgggtg gcctcgtctg cttggccggg accctcgtcg ttgctctgct
     4861 ctcgcccctg atgcgctacc gcaaaccccc gaccgaccaa gcggggggca ggaccggggg
     4921 gcccggtgaa gaaccctcga ataggacata acgacctaca agggggtctg cgtgaaggtg
     4981 tggcgaggct gcgttgccgt gagtctcgtc cttctgatgg ccggtgcggc gtggccggcc
     5041 ggagccactc ccgtcgccaa gaccaaggcc gagcgagagt ccctggggcg ttcgttcctg
     5101 gagcccctcg agtccacgaa cttcatccag ctcggcgacg agcccggtga gcacgagttc
     5161 gctccggctt tccagctgct cgagaagctg tttccccggt acgccgagta cacgaccgtc
     5221 gacgaggaac tgaacgaccc caacgcagtg agcgtcggcc ccgatggctt tccggcatgg
     5281 gacaagaagg acaccggaga tgggctcccg ttcgacgtcg cctacgtcac agacaaatcc
     5341 gttcccgacc acaagaaggg atacgtactg ctcaccgtcg ctcactctgc cgaaccgtgc
     5401 ggccgtgaag gcgcgatcag gttcctcgaa gaccttctga tctggcggac gtcggatccg
     5461 gatcacaagc tcaccgacca gtcgggcctc aaaggcgaag agcacaagat cacggtcgag
     5521 gaacttctga agagaaccaa gatctacttc gtgaacgtcg cgcccgacgg atggaccgcg
     5581 ggggacggct acgggaacat gtacgaccag tccaa
//
LOCUS       DATMED010000024         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5006566, whole genome shotgun sequence.
ACCESSION   DATMED010000024 DATMED010000000
VERSION     DATMED010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5006566"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VNP73_00575"
     CDS             complement(<1..330)
                     /locus_tag="VNP73_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWL64445.1"
                     /translation="MVQRREGLATWRGFLFAYRSLIDRLQAELQAERGLMLTWLDVLF
                     QLSFAPERRLTMRALADSVVLSPSGLTRVVDKMEEAGLVARERCEYDRRMWFIVLTDE
                     GAEELRVS"
     gene            405..950
                     /locus_tag="VNP73_00580"
     CDS             405..950
                     /locus_tag="VNP73_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019156083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWL64446.1"
                     /translation="MAWQLDPAHTQVGFTARHMGLSKVRGRFTNFDGTIDLDPANPTS
                     ARGRVAIDLASIETGNADRDAHLRSPDFFDVDNHPKMVFDVKSLAPAGDDHYKVVGDL
                     TIKGTTNQIELDLEYTGEGTDPYGNRKVGGSLSGTINRSDWGLTWNVALETGGWLVSD
                     KIKIEIEGQLAEAVKTERSAA"
     gene            973..1896
                     /locus_tag="VNP73_00585"
     CDS             973..1896
                     /locus_tag="VNP73_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL64447.1"
                     /translation="MKLEGIHHVTAITGDAPQNVEFYAGVLGLRLVKKSVNQDDPTIY
                     HLFYADEAGSPGSDITFFEYPGAPRGRAGDGMIHRVVFRVASADALDFWQNRIEKEGL
                     STERSDDSLVFPDPEGLDLEFVIDGSGDAPLEARFPHIPQEFAIRGFEGVRAFSSMPE
                     ASKELFEDTLGFQPKGDSHWTVRGNQRGGFYRFDAPPQVRPLQGAGTVHHVAWASEMD
                     DHTAWRERVLAAGMSPTPVIDRFWFRSVYFREPSGVLFEIATLGPGFTADEELEHLGE
                     RLTLPPNFEHLRDKLKGVLTPLPDPRARVGS"
     gene            1948..>3246
                     /locus_tag="VNP73_00590"
     CDS             1948..>3246
                     /locus_tag="VNP73_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL64448.1"
                     /translation="MASRRRPVATAAIVLILAVSALPSASAAASCKGKRATHDFSGMV
                     GVRFSGTPKADVIVGSSAGDELFGKGGNDTICSGGGGDFVIGGPGNDTLALGDDDDYT
                     LADAGKDSYDGGGGLDGVDYFQTSAALVADLAKDTVVAKGVTEKIKSVEVIWGSKGND
                     RLLGDNGENYLFGWDGNDRILGKGGNDFTTGDLGDDVLDGGTGELNGISYADSGTPVS
                     ADLSAGTATGEGSDTFSNFNILNGSRHDDTLVGSDGGEHIIGGDGEDNMQARGGDDFV
                     YADNGDDTIEGGDGLDTISYWFLARLVKVDLGEGSTVEGHEFFNQDFDTFTSIEAAIG
                     SDYEDELIGSSADDILDGGDGNDILTGLGGDHDVLRGGAGDDTINGGDGDADLATFSE
                     ATAGVTADLNAGSATGQGSDSLVDIESLMGGAGNDRLTGDG"
BASE COUNT          642 a         1003 c         1046 g          555 t
ORIGIN      
        1 ggagacgcgt agttcctccg ccccttcatc cgtcaacacg atgaaccaca tccgccggtc
       61 gtactcgcag cgctctctcg ctaccaggcc cgcttcctcc atcttgtcga cgacgcgggt
      121 cagcccgctg ggggacagga ccaccgaatc tgccagtgcc ctcatcgtca gcctgcgttc
      181 cggagcgaaa ctcagctgga acaagacgtc cagccacgtg agcattaagc ctcgctccgc
      241 ctgcagctcg gcttggaggc gatcgatgag cgagcggtag gcgaagagaa accctctcca
      301 tgtggccaga ccctctcgtc gctgaaccat ccgggaatac tcctctaata gttgtgttta
      361 caaccattgt atagcgaagt atcgctctag caaggaggac gcagatggct tggcaactag
      421 acccggcaca cacacaggtc ggattcaccg cccgtcacat gggcctttcc aaggtccgcg
      481 ggcgtttcac gaacttcgac gggacgatcg accttgatcc tgccaatccc acttcggctc
      541 gaggccgtgt tgcgatcgac ctggcgagca tcgagaccgg gaacgccgac cgggacgccc
      601 accttcggag cccggacttc ttcgacgtcg acaaccaccc caagatggtc ttcgacgtga
      661 agtctctcgc tccggccggc gacgaccact acaaggtggt cggcgacctg acgatcaagg
      721 gcaccaccaa tcagatcgag ctggacctcg agtacacagg tgaagggacc gacccctacg
      781 gcaaccgcaa ggtcggcgga agcctgagcg gaacgatcaa ccgcagcgat tggggtctca
      841 cgtggaacgt cgcgctcgag accggcggct ggctcgtgtc cgacaagatc aagatcgaga
      901 tcgaagggca gctcgcagag gccgtcaaga ccgaaagaag cgctgcttag tatcaatcca
      961 ggaggtgacg ggatgaagct cgaggggatc caccacgtaa cggcgatcac cggagatgct
     1021 ccgcagaacg tcgagttcta cgccggcgta ctcggacttc gtctcgtcaa gaagtcggtc
     1081 aatcaggacg atccgacgat ctatcacctg ttttacgcgg acgaggccgg cagtccgggc
     1141 tccgacatca cgttcttcga gtatccgggc gcgccgcgag gccgcgccgg tgacggaatg
     1201 atccatcggg tcgtgttccg cgtggcgtct gccgatgcgc tcgatttctg gcagaacagg
     1261 atcgagaagg aaggtttgtc gaccgagcgg tccgacgatt cgctcgtgtt ccccgatccg
     1321 gaaggcctcg atctcgagtt cgtgatcgac ggctccggtg acgcgccgct cgaggcgcgg
     1381 ttcccgcaca tcccccagga gttcgcgatc cgtggattcg aaggagtgcg cgcattcagc
     1441 agcatgccgg aggccagtaa ggagttgttc gaggacaccc tgggcttcca acccaagggc
     1501 gatagccact ggacggtgcg ggggaaccag cgcggaggct tctatcgctt cgatgcccct
     1561 ccgcaggtcc gtccactcca aggtgccgga acggttcacc acgtcgcgtg ggcgtccgag
     1621 atggacgatc acaccgcgtg gcgcgagcgg gtccttgcgg ccggaatgtc gccgaccccg
     1681 gtgatcgatc gtttctggtt ccgctcggtc tacttccgtg agccttcggg ggtcctgttc
     1741 gagatcgcaa cgctgggacc ggggttcacg gccgacgagg agctcgagca cctcggcgag
     1801 cgattgaccc tgccccccaa cttcgagcac ctccgggaca agctgaaagg cgtgctcacc
     1861 ccgctgcccg atcctcgagc tcgggtcggc tcctaaacgc ccatccccct tccaccggac
     1921 ggggtgcctg aatatcctgg ctcggtgatg gcttcccgca ggcgtccggt cgcaaccgcc
     1981 gccatcgtgc tgatcctggc ggtgagcgca ctcccatccg cctccgcggc tgcttcctgc
     2041 aagggcaagc gagcaaccca cgacttcagc gggatggtgg gggtcaggtt ctcgggcact
     2101 cccaaagccg atgtcatcgt gggcagttcg gccggggacg agctcttcgg caaaggcggg
     2161 aacgacacga tctgttccgg cgggggtggg gacttcgtga ttggggggcc gggcaacgac
     2221 actctcgcgc tgggcgacga cgacgactac acactcgccg acgccgggaa ggattcctac
     2281 gacggtggtg gcggattgga cggcgtcgac tacttccaga cctcggccgc gctcgtcgcg
     2341 gacctcgcca aagacacggt cgtagcgaag ggcgtaaccg agaagatcaa gagcgtcgaa
     2401 gtcatctggg ggtcgaaggg caacgaccgg ttgctcggcg acaacggaga gaactacctg
     2461 ttcggctggg acgggaacga ccggatcctc ggtaagggcg ggaacgattt cacgacaggc
     2521 gatctcgggg acgacgttct cgacggaggc accggcgaac tcaacggcat cagttacgcc
     2581 gactccggga cacccgtcag tgcggatctc tccgccggga cggcaacggg cgaaggtagt
     2641 gacacgttct cgaacttcaa catcctgaat gggtcgagac atgacgacac gctggtgggt
     2701 tccgatggtg gcgagcacat catcggaggc gacggcgaag acaacatgca ggcgcgaggt
     2761 ggagacgact tcgtctatgc agacaacggc gacgacacga tcgagggcgg tgacgggctc
     2821 gacacgatca gttactggtt ccttgcccga ctcgtgaagg tcgatctggg tgaaggcagt
     2881 accgtcgagg gtcacgagtt cttcaatcaa gacttcgaca cgttcacctc gatcgaagcg
     2941 gcgatcggtt cggactacga agacgaattg atcggctctt cggccgacga catcctcgac
     3001 ggcggtgacg gcaacgacat cttgaccggg ctgggcgggg accacgacgt tttgagaggc
     3061 ggcgccggtg atgacacgat caacggcggc gacggagatg ccgacctggc tacgttctcg
     3121 gaagcgacgg caggcgtgac cgcggatctg aacgccggtt cggcgacggg tcagggctcg
     3181 gactccctgg tcgatatcga aagccttatg ggaggggctg ggaacgaccg gctcaccggc
     3241 gacggc
//
LOCUS       DATMED010000025         7806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2707191, whole genome shotgun sequence.
ACCESSION   DATMED010000025 DATMED010000000
VERSION     DATMED010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2707191"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VNP73_00595"
     CDS             complement(<1..1039)
                     /locus_tag="VNP73_00595"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HWL64449.1"
                     /translation="MKTKLLSLFTALSTLLVVIPAGQAAAQDVPPVLPVERACKAEAP
                     VCAGYGQADATWHVGAGAGQYSRKNGDNVVNSVTGGDVDPHGHSILQSDSHGVHSRLS
                     YRAIVVEDEQGDQVAFVKADSYLSMDYLLRRAGQILLQNQSKISYEEIFHMASHNHSS
                     PYYMTPSPGPWVFQDAFDIRAFEYHARQLASAILAAEKALVPARMGATTVDHKLFKGM
                     VQRKRFADDGTPGGYPDDFGDFGLSVVRFDDISVPSAPKPLATLINYGQHPEGLDDHD
                     LITGDFVAPLERFVERATGAPLVFGQGDVGSAESGPGHPELVAEFGIPTRWSHAGHAQ
                     GERGAYLLSLDV"
     gene            complement(1081..3678)
                     /locus_tag="VNP73_00600"
     CDS             complement(1081..3678)
                     /locus_tag="VNP73_00600"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HWL64450.1"
                     /translation="MRRLSMCLCALVVMLGLMPAPATAQLAPKEPVDYVDPMIGTLFG
                     GFSFPGPAAPFGMVQLSPDTEGEFAYTGYQYGDTSIRGFSHHHIQSMGVHSNGDLPFM
                     PTVGPVLSEDPRSWMSPFDKATEEAQAGYYRVLLERYGILAELTAGHRVGMHRYTFPP
                     SPESNVIFDVEHSATGWHPHQPETNDGSVEVLDEKTIVGTGHDRRGIYNIHFAARFDR
                     DFTSFTPLGKGGYATFDTTADRELIVEVGISFVSRANALENLDAELAGNHFDFDTLRE
                     KTRADWNEALQTISLEGGTDLEKTSFYTALYHAQHHPNIYEDANGQYTGHDGRTHDSD
                     GYTHYANFSLWDTYRGENQLLATIQPDRYSDMMRSLLHMYREAGRFPQWAMNNAMPDY
                     MEGDPIQPTVVDGYCRGLLADDLIDEMYAALRSQAWDEGGKRSATESGYQEYHQNGYM
                     SGRGADASGNFEYAIADFSLALMADSLGKTADRDELLTRADNYKELINPTTGFAHPRE
                     ANGDWMAGYDPIAPDYWKEGTGWQYTWLAPQDLAGLFEIVNGNETGETVEDRLDTYFS
                     TDAADNPAVAEAQKTMTAFGVAYVGNQHAPSNEHDVHAPYLYDYAGLPWKTQKIMRGY
                     QLLFRPTPDGLPGNDDLGSMSAWFVWSALGFYPVQGGAPVYSVGSPMFPKATIAPIGR
                     EDEVVVEAPNASLPNKYIQSAELGDDALDRPWFTHDELFSSGRVAFEMGALQNEGWGA
                     DDPPPSLATHDLKSFGCPARSIEEPPDPVTTQLIYTGDTRGNGDEARLAARLVTVEGS
                     PVPGHTIVFEIAGGTFQAVTGADGVAATTVSVPNHGKSQTVLVRFAGNESYSPSATSA
                     TITWGKSKS"
     gene            complement(3715..4200)
                     /locus_tag="VNP73_00605"
     CDS             complement(3715..4200)
                     /locus_tag="VNP73_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012886862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWL64451.1"
                     /translation="MKVAVVADTHTHGMSRTIPMGAWPYLETADHILHAGDVTDPAVL
                     GELKALAPVTAVMGNCDSFDIRDWGATDEVEIEVGGISIAMLHDSGPTKGRRDRMRKR
                     FPGARVVVFGHSHMPFNEDENGLLLFNPGSPTWPRRAPFTSMGLLWIENGTVEGEIFP
                     V"
     gene            complement(4214..4723)
                     /locus_tag="VNP73_00610"
     CDS             complement(4214..4723)
                     /locus_tag="VNP73_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64452.1"
                     /translation="MENDIDLATYVASGMGRDHTDNSFVRFISGEPFVEFGSVDSRLL
                     DKAKENIQNHFVFVGTVDHFAESVLILKKEMGWRVPIFYGRHNVTQGRPAIESLDQKT
                     RAVLDRYTEADRALVDHCTALFREKLEAMPEDFELEVRRFRRRNAWVEKAYPKIQPTR
                     QRIRSALSR"
     gene            complement(4690..5013)
                     /locus_tag="VNP73_00615"
     CDS             complement(4690..5013)
                     /locus_tag="VNP73_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64453.1"
                     /translation="MPRTSGSTLNSILTRQYRRREIHVEELFNPSDIERFVALPESYR
                     RRIRLLKGHMAFGLHRQLPQPARYFTMVRDPIGWFRCTTTSDGDRKLDSTQRSWKTTS
                     ISLLT"
     gene            complement(5055..6191)
                     /gene="moeB"
                     /locus_tag="VNP73_00620"
     CDS             complement(5055..6191)
                     /gene="moeB"
                     /locus_tag="VNP73_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5271..5273),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWL64454.1"
                     /translation="MANFRDLLKQVKQEVGEVTAEDVRARLDAGERFHLLDVREADEV
                     QHGIVPGAKTLSRAHFESQAEDVLPDKDAEVIAYCATGVRSVFAAKTLRELGYTNVVS
                     MKGGFVRWKDLGYSFDIPRVLDRHQKDRYSRHLILPEVGLEGQMKFLDSKVLAIGAGG
                     LGSPALLYLAAAGVGTIGIVDSDVVEENNLQRQVVHNTDRIGMLKAESAKETIEKLNP
                     DVKVNVHAFRIDESNAEELMSQYDVVVDGCDNFDTRYVVNDAAVKLRKPVIHGSIFRF
                     EGMASTFIPYEGPCYRCLYPEAPPPELAPTUSEAGVLGVLPGIVGCLQANEALKLIAG
                     YGQPLVGRLLTFDAQSTSFQEVKVRRDPACPVCGEEPPALRAAS"
     gene            complement(6238..6489)
                     /locus_tag="VNP73_00625"
     CDS             complement(6238..6489)
                     /locus_tag="VNP73_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWL64455.1"
                     /translation="MTTNTTTTVTMYSTVWCGYCRRLKRQMTEAGIPFEEIDVDRVPG
                     YDARIIQASGGYRTVPTLEVGGQLLVNPTIKEVEAALAA"
     gene            6556..7611
                     /locus_tag="VNP73_00630"
     CDS             6556..7611
                     /locus_tag="VNP73_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWL64456.1"
                     /translation="MKAVVYDGAGGVRVDDVPEPKIERSTDALIEVTRGAICGSDLHI
                     LTGKTPGVQNGDVIGHEFVGVVKEVGDEVNAFTAGQRVIGSFLIACGSCGHCGAGRFN
                     LCGKRRAIGFGALTGGLGGSQAELVRIPKADINLHAIAGDYAELDDEQALFGGDILTT
                     GFYGAARSEINTDDVVVVVGAGPVGLFCAGAAKRYSPAKVIVLDQDANRVAFARDEMG
                     LDALDVSSDDPAKAVLEATGGRAADIAIEAVGHSSALETAMAVIRDGGRVTVIGVYGV
                     DRYEMPMGLCWVRGIDLRFAGMANVQGHWDEALLAVAKGEIDPTKIVSHRLPLDDAVE
                     GYELFRSREALKVVLTP"
BASE COUNT         1385 a         2354 c         2542 g         1525 t
ORIGIN      
        1 cgacatcgag ggacaacagg tatgcgccgc gctccccttg tgcatgaccg gcatgcgacc
       61 agcgcgtcgg gatgccgaac tcggctacga gctcggggtg cccggggccg gattccgcac
      121 ttccgacatc gccctgaccg aacacgagcg gtgcacccgt cgcgcgctcg acgaagcgct
      181 ccaacggagc aacgaaatcg ccggtgatga ggtcgtggtc gtcgagacct tcgggatgct
      241 gaccgtagtt gatcaaggtg gcgagaggct tgggagcact gggcacggag atgtcgtcga
      301 accgaacgac ggatagaccg aagtcaccga agtcgtccgg gtaaccaccg ggagtcccgt
      361 cgtccgcgaa gcgcttgcgc tgaaccatgc ccttgaagag cttgtgatcg acagtggtag
      421 cacccatccg tgcagggacg agcgccttct cggccgccag gatcgcgctg gccaattggc
      481 gggcgtggta ttcgaacgcg cggatgtcga acgcgtcctg gaacacccag ggacccggcg
      541 acggcgtcat gtagtaaggc gacgagtggt tgtggcttgc catgtggaag atctcctcgt
      601 atgagatctt cgactggttc tgcagcagga tctgacctgc acgcctcagc aggtagtcca
      661 tcgataggta actatctgcc ttgacgaacg cgacctgatc accctgctcg tcctcgacca
      721 cgatcgcgcg gtaactgaga cgcgagtgga cgccgtggct gtcgctctga aggatcgagt
      781 gaccgtgggg gtccacgtct ccaccggtca cggaattgac cacgttgtcc ccgttcttcc
      841 gcgagtactg accggccccc gcacccacgt gccaggtcgc atctgcttgg ccgtagccag
      901 cacagaccgg ggcctcggct ttacaggcac gctcgaccgg aaggaccggg gggacatcct
      961 gagcggcggc ttggccggcc gggatgacaa ctaagagggt tgaaagcgcg gtgaacaggg
     1021 aaaggagttt tgttttcatg gcgttttctt tcgccgcctt acgccgtagt cctgcggcaa
     1081 ctagctcttc gacttgcccc aagtgatcgt tgccgaagtc gcgctcggtg aataactctc
     1141 attcccggca aaccggacaa gcaccgtctg cgacttcccg tgattcggaa cggagacagt
     1201 cgtcgcggcg acgccgtcgg ctccggtcac ggcctggaac gttccgccgg cgatctcgaa
     1261 gacgatggtg tgtccgggca ccggggaacc ctctacagtg accaagcgcg ccgcaagtcg
     1321 agcctcgtcg ccattgcctc tcgtgtcgcc ggtgtagatc agttgcgtgg tgacggggtc
     1381 ggggggctcc tcgatgctcc gagcgggaca accgaaactc ttcaggtcgt gtgtggccag
     1441 cgatggaggc gggtcgtcgg cgccccagcc ttcgttctgg agtgcgccca tctcgaatgc
     1501 gacccggcct gacgagaaca actcgtcgtg ggtgaaccac ggtctgtcca atgcatcgtc
     1561 gccgagctcg gccgactgga tgtacttgtt gggcagtgac gcgttgggag cctcgacaac
     1621 gacttcatct tcccggccga tcggcgcgat cgttgccttc gggaacatcg ggctgcccac
     1681 cgaataaacg ggtgctcctc cctgtacggg atagaagccg agcgcgctcc acacgaacca
     1741 ggccgacatc gaaccgaggt cgtcgttccc cggaagcccg tcgggtgtcg ggcggaagag
     1801 aagctggtag ccgcgcatga tcttctgcgt cttccacggc aagcccgcgt agtcgtagag
     1861 gtaaggagca tgaacgtcgt gctcgttgct cggggcgtgc tggttgccca cgtacgcgac
     1921 cccgaaggcg gtcattgtct tctgcgcctc ggcgactgcc gggttgtcgg ctgcgtccgt
     1981 tgagaagtag gtatcgaggc gatcctcgac ggtctcaccg gtctcgttgc cgttcacgat
     2041 ctcgaacaat ccggcgaggt cctgcggagc gagccacgtg tactgccagc ccgttccctc
     2101 cttccagtag tcgggagcga tcgggtcgta gccggccatc cagtcgccgt tggcctcgcg
     2161 tggatgggcg aagccggtgg tgggattgat cagctccttg tagttgtcgg cgcgagtcag
     2221 cagctcgtct cgatcggccg tcttgcccag agagtccgcc atcaaggcca gagagaagtc
     2281 ggcgatcgcg tattcgaagt tgccggacgc atccgcaccg cgacccgaca tgtaaccgtt
     2341 ctggtggtac tcctggtagc cgctctccgt cgcagagcgc ttaccgcctt catcccaggc
     2401 ctggctgcga agcgccgcgt acatctcgtc gatcaggtcg tcggccaaga gaccgcggca
     2461 atacccatcc acgacggtgg gctggatcgg atctccctcc atgtaatcgg gcatcgcatt
     2521 gttcatcgcc cactgtggga agcgacccgc ctcgcgatac atgtgcagga gcgagcgcat
     2581 catgtcggag tacctgtcgg gctggatcgt cgcgaggagc tggttctcgc cacggtaggt
     2641 gtcccacagc gagaagttcg cgtaatgcgt gtacccgtcc gagtcgtggg tccgaccgtc
     2701 gtggcccgtg tactgcccgt tggcgtcctc gtagatgttc gggtgatgct gtgcgtgata
     2761 cagggctgtg tagaaggacg tcttctcgag gtcggtccca ccctccaagg agatcgtttg
     2821 cagggcttcg ttccagtcgg cgcgtgtctt ctcgcgcaga gtgtcgaagt cgaaatggtt
     2881 accggcgagc tcggcgtcga gattctccag tgcattcgcc cggctcacga acgagatccc
     2941 gacctcgacg atcagttccc ggtcggcggt cgtgtcgaag gtcgcatagc cgcccttgcc
     3001 gagaggcgtg aagcttgtga agtcacgatc gaaacgggcc gcgaaatgga tgttgtagat
     3061 accgcggcga tcgtggcccg taccaacgat cgtcttctcg tccaggacct cgaccgatcc
     3121 gtcgttcgtc tcgggttgat gcggatgcca accggtcgcc gagtgctcga cgtcgaagat
     3181 cacgttggac tcagggctcg gcgggaacgt gtagcgatgc atgccgaccc tgtggcccgc
     3241 ggttagctcg gcgaggatcc cgtagcgctc caggagcact cggtagtagc cggcctgagc
     3301 ctcttcggtg gccttatcga aaggagacat ccacgagcgg gggtcttcgg agaggacggg
     3361 cccgaccgtc ggcatgaagg gaaggtcgcc gttgctgtgc acgcccatcg actggatgtg
     3421 gtggtgtgag aaacctcgga tcgaggtgtc gccgtactgg tagccggtgt aggcgaactc
     3481 cccttcggtg tcggggctga gctggaccat gccgaagggg gccgcggggc cggggaacga
     3541 gaagcccccg aacagcgtgc cgatcatcgg gtcgacgtag tcgacgggtt ccttgggagc
     3601 cagctgcgcg gtcgcggggg ccggcatgag gccgagcatc acaacgagcg cgcacagaca
     3661 catcgacaga cgccgcatgg cgtgaaggtt cgccgcgacc gcttgcttcg cctgctagac
     3721 cgggaagatc tcgccctcca ccgtgccgtt ctcgatccac aggagcccca tcgaggtgaa
     3781 cggagcccgc cgcggccacg tcgggctccc ggggttgaac aagaggaggc cgttctcgtc
     3841 ctcgttgaac ggcatgtggg aatgaccgaa aaccacgacg cgggccccgg ggaaacgctt
     3901 gcgcatgcgg tcgcgccggc ccttggtggg gccggagtcg tgcagcatcg cgatcgatat
     3961 cccgccgacc tcgatctcta cctcgtcggt cgctccccaa tctcggatgt cgaacgagtc
     4021 gcagttgccc atcacggccg tgacgggtgc aagggccttg agctccccca gaacggccgg
     4081 atcggtcacg tccccggcgt gcaggatgtg atcggcggtc tcgaggtagg gccatgcccc
     4141 catcgggatc gtgcggctca tcccatgcgt gtgcgtatct gcaacgacgg cgaccttcat
     4201 ctgcccggta gacctatctg gacagggcgg atctgatccg ctgacgagtg ggctggatct
     4261 ttggataggc cttctcgacc cacgcattac gccgtctgaa ccttcgcacc tcgagttcga
     4321 aatcttcggg catagcctcg agtttctctc ggaacagcgc ggtgcagtgg tcgacgagcg
     4381 ctctgtcggc ctcggtgtat cgatccagca cagccctggt cttttgatcg aggctctcga
     4441 tcgcaggccg accttgagtc acgttgtggc gcccgtaaaa gatcggcacc cgccagccca
     4501 tctctttctt caagatcaga acggactcgg cgaagtgatc gaccgttccg acgaacacga
     4561 aatggttctg gatattctcc ttggctttat ccagaaggcg cgagtcgacc gagccgaatt
     4621 cgacgaacgg ttcacctgag atgaaccgca cgaaggagtt gtcggtgtga tccctgccca
     4681 tcccgctggc tacgtaagta gcgagatcga tgtcgttttc catgatcgtt gggtggagtc
     4741 gagcttccgg tcgccgtcgg atgtagtagt acatcgaaac cacccgatcg gatcgcgaac
     4801 catggtgaag tagcgcgccg gttgggggag ttgacggtgg aggccaaaag ccatgtggcc
     4861 ctttagcagg cggatccgcc gccggtagct ctcgggcagc gccacgaatc gttcgatgtc
     4921 ggaggggttg aacaactcct ctacgtggat ctctctccga cggtactggc gcgtaaggat
     4981 gctgttgagc gtggatccgg acgtgcgtgg cacgtgcagg aagatcaaag cttggtcggc
     5041 cgcgtggccc ctcgtcaaga cgcggctcgg agcgccggcg gttcctctcc acatacaggg
     5101 caggccgggt cgcggcggac ctttacctct tggaacgaag ttgactgcgc gtcgaacgtg
     5161 agcagacgac cgaccagagg ttgtccgtaa cccgctatca atttgagtgc ctcgttggct
     5221 tggagacacc cgacgatgcc tgggagcacg cccaggaccc ctgcttcgct tcaggtaggg
     5281 gccagttcgg ggggcggcgc ctcgggatag agacaccgat agcacggacc ttcatatggg
     5341 atgaaggtcg atgccatccc ctcgaaccgg aagatcgatc cgtggatcac cggcttgcgt
     5401 aacttcacgg ccgcgtcgtt caccacgtag cgcgtgtcga agttgtcaca cccatcgacg
     5461 acgacgtcgt attgcgacat cagctcttcc gcgttcgact catcgatgcg gaacgcatgg
     5521 acgttcacct tgacgtcggg attgagcttc tcgatcgtct ctttcgccga ctcggctttc
     5581 agcatcccga tgcgatcggt gttgtgcacg acctgccgtt ggaggttgtt ctcttcgacg
     5641 acgtcggagt ccacgatccc gatcgtgccg acgccggcgg ccgccagata gaggagagcg
     5701 ggagagccga ggcccccggc cccgatcgcg agcaccttcg agtcgaggaa cttcatctgg
     5761 ccttcgagcc cgacctcggg aaggatcagg tggcgcgaat agcggtcttt ctggtggcgg
     5821 tcgagcacgc gcgggatgtc gaaggagtaa ccgaggtcct tccaacggac gaagccgccc
     5881 ttcatcgaca cgacattcgt atacccgagc tctcgcaggg tcttggcggc aaagacgctt
     5941 cgcacgccgg tcgcgcaata cgcgatcacc tctgcgtcct tgtcgggaag tacgtcttcg
     6001 gcctgggatt cgaagtgggc tcgggaaagg gtcttggccc ccggcacgat gccgtgctgg
     6061 acctcgtcgg cctcgcgcac gtcgaggagg tggaagcgtt ccccggcgtc gaggcgagcc
     6121 cggacgtctt cggcggtgac ctcgccgacc tcttgcttga cctgcttcag aaggtcacgg
     6181 aagtttgcca tattaatacc ttacaatacg tgtaaatagg ctggggcgct gcctagatta
     6241 tgcagcgaga gcggcttcga cctctttgat cgtggggttc acgaggagct gcccacccac
     6301 ttcgagggtc gggaccgtcc ggtagccccc ggaggcctgg atgatccggg cgtcgtatcc
     6361 gggcacacga tcgacgtcga tctcctcgaa ggggatgccc gcctccgtca tctggcgttt
     6421 cagacggcgg cagtagccgc accagacggt ggaatacatc gtgacggtag tggtcgtatt
     6481 ggtagtcatg tccgtttagt tgtagacaac caagcctatg ccgggtcatt cccctagcga
     6541 tgcaaggacg aaaccatgaa ggcggtcgtg tacgacggag ccggtggggt cagggtggac
     6601 gacgtccccg agccgaagat cgaaagatcc accgatgccc tgatagaggt gacccggggt
     6661 gcgatctgcg gatcggacct ccacatcctt acgggcaaga cgcccggcgt tcagaacggc
     6721 gacgtcatcg gtcacgagtt cgtgggtgtg gtcaaagagg tcggcgacga ggtgaacgcg
     6781 ttcaccgccg gccagcgggt gatcggctcg ttcctcatcg catgcggatc gtgtgggcat
     6841 tgcggggcgg gacgcttcaa cctgtgcggc aagcgtcggg ccatcgggtt cggcgccctg
     6901 accggaggcc tcggcggctc gcaggccgag ctcgtgcgta tcccgaaagc agacatcaac
     6961 ctgcacgcga tcgctggtga ctatgcggaa ctcgatgacg agcaagcctt gttcggcgga
     7021 gacatcctta ctaccggctt ctacggagcg gcacgttctg agatcaacac cgacgatgtg
     7081 gtcgttgttg tgggagcggg tcccgtcgga ctcttctgtg ccggagcggc caagcgttat
     7141 tcaccggcca aggtcatcgt gttggaccag gatgcaaacc gcgtagcgtt cgcgcgcgac
     7201 gagatggggt tggacgctct ggatgtgtcg agcgatgacc cggctaaggc agtcctcgaa
     7261 gcaaccggag gtcgcgccgc agacatcgcg atcgaggccg ttggtcactc ctccgctttg
     7321 gagactgcga tggctgtgat ccgcgatggg ggccgcgtca ccgttatcgg tgtctacggc
     7381 gtcgatcgtt acgagatgcc gatgggcttg tgctgggtgc gcgggatcga cctacgtttc
     7441 gccgggatgg cgaacgtgca aggtcactgg gacgaggcgc tactcgccgt ggcaaagggg
     7501 gagatcgatc caacgaagat cgtgtcgcac cgcctgcccc tcgacgacgc agtcgagggt
     7561 tacgagttgt tcagatcacg cgaagccttg aaggtggtcc tgacgcctta ggcagatctc
     7621 gcgccgcgcg aggcctcatg tttctctttc tcggccacga tctcgctcgc gctcttcgag
     7681 caaccggggc agaacttgga gtcggccgga acgttcgcgc tgcagaacgg gcaaggcccc
     7741 tcgacctcct gctccttata gccctccttc tagccctctt tgaactcctt gccggcctgg
     7801 cccatc
//
LOCUS       DATMED010000026         6118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2710365, whole genome shotgun sequence.
ACCESSION   DATMED010000026 DATMED010000000
VERSION     DATMED010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710365"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..3081)
                     /locus_tag="VNP73_00635"
     CDS             complement(265..3081)
                     /locus_tag="VNP73_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL64457.1"
                     /translation="MFESFVAVPAHFTVEFLGFLVFAGGAILSLSRSELVPGETSNRL
                     SAAVGFAALAAAQVLHGGSFGGAETDGTRVLIGLEAVGLAFIFVGVVGSLKAAATAAA
                     VAPGSWRLQDPLLLAPAAVGLVLFFAAVAGSRSTRTLRRLAAFALLFTVAAALTAGAP
                     GAEFGTRTVSSVAYTAHALKFVSFIALGSWMWSVVRSSIRTRFVASFAVLLVVVVMAL
                     STALTGVISTNVEVEELRQLSTQVRSAVSQIAQSRAEELNDDASTIAEDLPEVKNRFL
                     ARADMNGLAQEVRGQGTFELENGFAMTMDPRGKVLGFSGQGPYRDQGKRLVPTQLRRI
                     DILTINGSGVIGEIADRGRKAAASVDLVGRSLVAVVAAHEVTHPSQPNRRTGIIAIGR
                     WVDAQTVEGLTSSLGSDASLLVHGRVLATDLPGKPDRKGLIPGSLRNQVRTCSGVVTG
                     QVAVGDLTFFSAFGCLRNAADDPVATIVLSSPAREIGRARGVFTRILFLIAMGVGAVS
                     LVLAWFSGRAITQPIQDLTAAAGAVREGNLQAEAPVKGEDEVGQLGETFNEMTASLFR
                     MTNDLRDAARQEHDLRARIEAIIESMADGLVAVDAEKRVLAFNPAAEEITGVASESAV
                     GKPIQDVLDARDAQGTRISLPIFELAQGSVGGIYLARKDTDPVPVSVVSAILPDVDGG
                     ASGGVAVLRDMTREREIERMKGEFLSNISHELRTPLTPIKGYAEILGRKDLPPEKTQK
                     FAAGILESTARLERIVHLLVDFAAMEAGRLSPKTKAVDLGSMVTDITDDWARRSPKHS
                     VVAEVDGAIPPVSGDERLLRRSIEELMDNAVKFSPRGGTVRVQVGGAGGNGRKEVQIT
                     ISDEGIGIPPEDMTKIFSDFHQLDGSETRAYGGLGLGLAFVRRIVEAHEGSIEVDSKP
                     EEGTRLTITIPAARGVSVASRSDD"
     gene            complement(3159..4208)
                     /locus_tag="VNP73_00640"
     CDS             complement(3159..4208)
                     /locus_tag="VNP73_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWL64458.1"
                     /translation="MTSHPWDLPDPPGTEPVRGIHPWLWLISALAVVGFLALAFFVPI
                     PIFFGYLPGPVRDVEDLVKVTDARTYSSEGSLYLTTVSVDTQVTIYDMVVSLFDDELN
                     IVMAEDVTQGQSLRRLQRQQKQEIVDSKRRAQEVALSALGFARPKGDGARVTGVLPNT
                     PAEGRLMPGDRILTIEGTRVSTACDVGRLVDSVDVGGRLTITVRRGGETREVSLRTAR
                     NPQDQMSSFLGIVMESVNYRFDPGFKIEFETGEIAGPSAGLMFSLALYDRLTPDDLTA
                     GREVAGTGTIDCDGGVGPIGGIEQKVAGAEAKGAEIFLAPQANSAAAQAVADEIEIVP
                     ISTFDDAVEYLEGLQ"
     gene            complement(4239..5237)
                     /locus_tag="VNP73_00645"
     CDS             complement(4239..5237)
                     /locus_tag="VNP73_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWL64459.1"
                     /translation="MARDLAIDLGTANTLVYQRQRGILFSEPTVLALNKDTGAVLAMG
                     DQAWQMIGRTPGYIVAVRPLRSGAISDFDVTEKLIRLILQKGGVTRFARPKVLVCVPS
                     ALTEVERRAVEEATLSAGARTCYLVEEPLAAAIGAGLPIHEPLGNLVVDVGGGTSEVA
                     LISMGGIVTNTAVRVGGFDLDSAIQNYVRREYTIAIGERTAERIKISIGSAFPLAQEQ
                     KADLRGRDLASGLPKNITITSEEIREAIAEPVRTIVAAVIECIGSSPPDLVQDVLSNG
                     VTLTGGGGLLNGLDMLISQQAEVPVHVTPQPLEAVVLGAGRCLESFESLKSMFVGA"
     gene            complement(5247..5558)
                     /locus_tag="VNP73_00650"
     CDS             complement(5247..5558)
                     /locus_tag="VNP73_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64460.1"
                     /translation="MIYLVMMMLVAGIGIGSLMMQQRRQRAHMESVEGFRNSLSKIAP
                     EATPGPFRKKHAVARPRPQGRTPGRPVPMDARRRAEARRRLEARRRSQMARAAASRNG
                     F"
     gene            complement(5742..>6118)
                     /locus_tag="VNP73_00655"
     CDS             complement(5742..>6118)
                     /locus_tag="VNP73_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64461.1"
                     /translation="APDDWLESNQIMSLLDRKRVDLPEPVAFAVAERLWSLGMAEAPA
                     GMLHYVMHPWVVSAEKLKSTGFVCTYSSEETFRQVIERLQGRIRIGNRSVPKNQVAAT
                     AAAAGLVGAFFATRRVLRRRAA"
BASE COUNT         1112 a         2045 c         1886 g         1075 t
ORIGIN      
        1 ttcaacgagg atggtttcgg caccgcgaac gatcgtgacc ttgccggcct gggctcggat
       61 attcatctcg ccgagctcct cgtccccgcc gaacgaccag caggcaccgg cggtgaaaag
      121 aacggcgacc aggggcagcg ccagcttctt catgggcgcc cgcgcaaaga taaggaggtg
      181 aaaacgccgc tcacatacgt agcattacgt ggcaaaccgg cctcgtgaat agctctttcg
      241 cactactttc atagtccttg aggactagtc atcggacctc gaggccaccg atacccccct
      301 ggcggccggg atcgtgatcg tgagccgggt gccctcctcc ggcttgctgt ccacttcgat
      361 ggagccctcg tgggcctcga cgatgcgtct cacgaaggcg agacccagcc cgaggccccc
      421 gtaagcgcgg gtctccgacc cgtcgagctg atggaagtcc gagaagatct tcgtcatgtc
      481 ctcgggcggg atcccgatgc cctcgtccga gatcgtgatc tgaacctcct tccgaccgtt
      541 gcctccggcc cccccgacct gaacgcgcac ggttcctccg cgcggagaga acttcacggc
      601 gttgtccatc aactcttcga tcgaccggcg cagcaggcgc tcgtctccgg acaccggagg
      661 tatcgctccg tcgacctcgg ccacgaccga atgtttggga ctccggcgag cccagtcgtc
      721 cgtgatgtcg gtaaccatcg agccgagatc cacagccttg gtcttcggtg agagccggcc
      781 ggcctccatc gcggcgaaat caacgagcag gtggacgatg cgttccagtc gtgcggtcga
      841 ctccaatatc cctgctgcga acttctgtgt cttctccggc ggcaggtcct tgcgcccgag
      901 gatctcggcg tatcccttaa tgggagtcag cggcgttcgc aactcgtgcg agatgttgga
      961 gaggaactca cccttcatcc gctcgatctc ccgttcccgg gtcatatctc tcagaacggc
     1021 gacgccgccc gaggccccac catcgacgtc cggcaggatc gcactgacga ccgagaccgg
     1081 aacggggtcg gtgtcctttc tcgccaggta gatgccgccc accgaccctt gggcgagttc
     1141 gaagatcgga agcgagatcc gggtgccttg tgcgtcgcgg gcgtcgagca catcctggat
     1201 cggctttccc acggcgcttt ccgatgcgac gccggttatc tcttcggccg cggggttgaa
     1261 cgcgagcact cgcttctctg catcgaccgc caccagcccg tcggccatcg attcgatgat
     1321 cgcttcgatc cgagcgcgga gatcgtgctc ctggcgcgcc gcgtcgcgaa gatcgttagt
     1381 catgcgaaac agggacgcgg tcatctcgtt gaaggtctct cccaactgac cgacctcgtc
     1441 ctcgcccttg accggcgcct cggcctggag gttcccctct cgtaccgcgc cggccgcggc
     1501 ggtcaagtct tggatcggtt gcgtgatcgc acgtcctgag aaccatgcga ggacgagcga
     1561 tacggccccc acccccatcg cgatcaggaa gaggatgcgg gtgaatacgc cccgagcgcg
     1621 gcctatctct cgggcgggcg acgacaacac gatggtcgct acggggtcgt ctgcggcgtt
     1681 cctcaggcat ccgaaagcac tgaagaaggt gaggtccccg accgcgacct gtccggtaac
     1741 gactccggaa catgtccgaa cctggttccg caacgatccc ggtatcaatc ccttgcgatc
     1801 gggtttgccg ggaaggtcgg tagcgaggac gcgaccgtgc accagcagag acgcatcgga
     1861 gccgaggctc gaggtgagac cctcaaccgt ctgcgcgtcc acccatcgac cgatagcgat
     1921 gatccccgta cgtcggttcg gctgagacgg atgcgtcact tcatgggcgg cgacgacggc
     1981 caccaggctg cgaccgacga ggtcgacgct ggcggcagcc ttccgccccc tgtctgcgat
     2041 ctcgccgatg acccccgaac cgttgatcgt caggatgtcg atccggcgca gttgggtggg
     2101 aaccagccgt ttcccttgat cgcgataagg cccctggcct gagaagccca acaccttccc
     2161 gcgcgggtcc atcgtcatcg cgaaaccgtt ctcgagctcg aacgttcctt gccccctgac
     2221 ctcttgagcg aggccgttca tgtcggcgcg cgctagaaag cggttcttca cctccggcag
     2281 gtcctcggcg atcgtgcttg cgtcgtcgtt cagctcttcg gccctgctct gagcgatctg
     2341 actaactgct gagcgcacct gcgtgctcag ctgtctcaac tcttcgacct caacgttcgt
     2401 ggagatcaca ccggtcaacg cggtagacag agccatcacg accacaacga ggaggacggc
     2461 gaacgacgca acgaagcgcg ttcggatact ggatcggacg acggaccaca tccacgagcc
     2521 gagcgcgatg aacgagacga acttcaaagc gtgagccgtg taggccaccg aagaaaccgt
     2581 tcgggtaccg aactcggccc cgggagcgcc ggcggtgagc gcagcggcga ccgtgaacag
     2641 cagggcgaag gccgccaatc ggcggagggt ccgggtcgac cgcgaccccg ccaccgcggc
     2701 gaagaacaga acgagtccca cggcagccgg ggcgagaagg agggggtctt ggagacgcca
     2761 gctgcccggg gccacggcgg ccgcagtggc tgccgctttc aggctcccaa cgacaccgac
     2821 gaatatgaac gcaagaccga ctgcctcgag accgatcaag acgcgggtgc cgtccgtctc
     2881 ggcccccccg aaggatccgc cgtgcagcac ctgagccgca gcgagcgcgg cgaatccaac
     2941 cgcagcgctc agccgatttg aggtctcccc cggcacgagc tcggaccggg ataaagacag
     3001 gatcgccccg ccggcgaaga cgaggaagcc gaggaactcg acggtgaaat gggcaggtac
     3061 tgccacgaaa ctctcaaaca tgttcgcgac ctagtgtcgc acgcaacctg tgttgtacgt
     3121 aggcaggggg cagccccctt aactgcctgg tcggagagct attgcagtcc ctccagatac
     3181 tcgacggcat cgtcgaacgt cgagatcgga acgatctcga tctcgtcggc cactgcctga
     3241 gcagccgccg agttcgcttg cggcgcgagg aagatctcgg cccccttggc ctcggcgccg
     3301 gcaaccttct gttcgatccc gccgatcggg ccgacgccac cgtcgcaatc gatcgtgccc
     3361 gtccctgcga cctctcttcc ggccgtgaga tcgtccggcg tcagccggtc gtagagggcc
     3421 agcgagaaca tcagaccggc cgaagggccc gctatctcgc cggtctcgaa ctcgatcttg
     3481 aatccgggat cgaacctgta gttgacgctc tccatcacga tcccgaggaa cgatgacatc
     3541 tggtcttgag ggtttcgcgc cgttctgagc gagacctcgc gggtctcgcc tcctcttcgc
     3601 accgtgatcg tcagccggcc cccgacgtcg accgagtcga cgagccggcc gacatcgcat
     3661 gctgtgctca ccctcgtccc ctcgatcgtc aggatccggt caccgggcat caagcggccc
     3721 tccgccggag tgttcggcag cacaccggtg acgcgcgctc catcgccttt cggtcgcgcg
     3781 aagccgagtg cagaaagcgc gacctcttgc gcccggcgtt tggagtcgac gatctcctgc
     3841 ttctgctggc gttgcagacg gcggagggat tggccctggg tgacgtcctc ggccatgacg
     3901 atgttgagct cgtcgtcgaa caagctcact accatgtcgt agatcgttac ctgcgtgtcg
     3961 acgcttacgg tcgtgagata gaggctgccc tccgatgaat acgtccgagc gtcggtcacc
     4021 ttaacgaggt cttcaacgtc tctgaccggg ccgggcagat aaccgaagaa gatcgggatc
     4081 ggcacgaaga atgcgagggc caggaaaccg accacggcca gtgccgagat aagccacagc
     4141 cagggatgga tcccgcggac gggctcggtt ccggggggat cgggaagatc ccaaggatgt
     4201 gacgtcactc cgaaacgata ggtcttacgc gcggcgcact aggcgccgac gaacatgctc
     4261 ttgaggctct cgaacgactc caagcagcgt ccggccccca gcacgacggc ttcgagaggt
     4321 tgcggggtga cgtgaaccgg gacctctgct tgctgagaga tcagcatgtc gaggccgttg
     4381 agaagacccc cacctcccgt gagcgtcaca ccgttcgaca acacgtcctg caccaggtcg
     4441 ggaggcgaag agccgatgca ctcgatgacc gctgcaacga tcgttctgac cggctcggcg
     4501 atcgcttctc tgatctcctc ggaggtgatc gtgatgttct tcggaagccc gctggccagg
     4561 tctcgtcccc gtaggtcggc cttctgctcc tgggccaggg gaaacgccga cccgatcgag
     4621 atcttgatcc gctcggcggt ccgctccccg atagcgatcg tgtactcgcg cctcacgtag
     4681 ttctggatcg cggaatccag gtcgaagccg ccgacccgca ccgccgtgtt cgtaacgatc
     4741 ccgcccatcg agatgagagc cacctcgctg gtgcctccgc ccacgtcgac gacgaggttc
     4801 ccaaggggtt cgtggatcgg caggcccgct ccgatcgccg ccgccagcgg ctcttcaacc
     4861 agataacagg tacgggcacc ggcggagagt gtggcttcct ctacggccct gcgttccacc
     4921 tcggtcaagg cactcggcac acacacgagc accttcgggc gcgcgaagcg cgtgaccccg
     4981 cccttctgaa ggatcaggcg gatcaacttc tcggttacgt cgaagtccga gatcgctccc
     5041 gaacgaagtg gccgaacggc cacgatgtag ccgggggtac gaccgatcat ctgccacgcc
     5101 tgatcgccca tcgccagaac ggcacccgtg tccttgttga gcgcgagcac cgtcggctcg
     5161 gagaagagga tcccgcgctg acgttggtaa acgagagtgt ttgcggtgcc gaggtcgatc
     5221 gcgagatcgc gggccactac gcgggcctag aaaccgttgc gcgaagcggc cgctctggcc
     5281 atctgcgaac ggcgacgtgc ctcgaggcga cgacgcgctt cggcgcggcg ccgggcatcc
     5341 atcggtacgg gccggccggg ggtgcgtcct tgaggccggg ggcgcgccac cgcgtgcttc
     5401 ttgcggaagg ggcccggtgt cgcctctggt gcgatcttcg acaaagagtt gcgaaagccc
     5461 tcgacggact ccatatgtgc gcgctggcgg cgctgttgca tcatgaggct tccgatgccg
     5521 atgccggcaa ccagcatcat cattactaag tagatcaagc ccgcgctcct atcaagtcgt
     5581 ttccttgtgg gggtgaccca accgttcagt gagcttcaat gctcccgaga tcggatccgc
     5641 caccttccta ggcggctgca ggcaggctaa cagaggcccg ccgtcccaca ggttctttgt
     5701 cgatatttca acaacggtgc cccgatacct ctatctactt atcaagcggc ccggcgacgg
     5761 agcaccctcc gggtcgcgaa aaaagcgcct accaggcctg cggcggcagc cgtcgcggca
     5821 acctggttct tcggcacgct cctattgccg atcctgatgc gtccctggag gcgctcgatc
     5881 acctggcgga aggtctcttc gctcgaatac gtgcacacga acccggtgct cttgagcttc
     5941 tcggcggaca cgacccaggg gtgcatcacg tagtgcagca tcccggcggg cgcctcggcc
     6001 attccgagcg accacaaccg ctccgcaacc gcgaacgcga ccggctcggg aaggtcgacc
     6061 cgcttcctgt cgagcagcga catgatctgg ttcgactcga gccagtcgtc cggcgcaa
//
LOCUS       DATMED010000027         9942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4629354, whole genome shotgun sequence.
ACCESSION   DATMED010000027 DATMED010000000
VERSION     DATMED010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4629354"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="hisC"
                     /locus_tag="VNP73_00660"
     CDS             <1..1007
                     /gene="hisC"
                     /locus_tag="VNP73_00660"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWL64462.1"
                     /translation="LPARYRMNTNESPYAPPDALVREVSNEIEAAALNRYPDRDAKAL
                     YRAISDHIDHPVEGLWVANGSNEVFMHLFLGFGGAGRTTLLFEPTYSVHSLIPRIAST
                     NVVPIDRGENFHIDVDEALDAIVEGRPDVVIVCSPNNPTGNCESIGTIEAILEAAPGL
                     VVVDEAYGEFAHPGETVRPLLKSHRNLVVTKTFSKAWRLAGVRIGYMMADPQLVNELQ
                     RVKLPYHLSTITQAVGVAAIRHSDETLRLVDALITERDRLAMGLEAMGLKVYPSRANF
                     VLFEIEDSERVWKELLERDVLIRNYSGERGLEDCLRVTAGLPEETDVFLSTLEEILDD
                     "
     gene            1000..1590
                     /gene="hisB"
                     /locus_tag="VNP73_00665"
     CDS             1000..1590
                     /gene="hisB"
                     /locus_tag="VNP73_00665"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWL64463.1"
                     /translation="MTERRAVVERTTSETKIRVELDLDGGAPSISTGVPFFDHMLDQL
                     ARHARVGLVVEAEGDLEIDAHHTVEDVGIALGEALAKALGDKRGIHRYGDALVPMEEA
                     LAQVALDISGRPLLLYTADFPAESIGQYDTVLTEEFLQALSRTAGLTLHVRLLEGRNA
                     HHAVEAIFKALARALGAAVSTDPRAGDDIPSTKGTL"
     gene            1587..2183
                     /gene="hisH"
                     /locus_tag="VNP73_00670"
     CDS             1587..2183
                     /gene="hisH"
                     /locus_tag="VNP73_00670"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWL64464.1"
                     /translation="MKVAVLDHGMGNLRSVAKAIEAAGADVTVTGEAADVQRSDALCV
                     PGQGIFGRCMENLTGTGMDDLIRSWIDSSRPYLGICLGMQVLFDRSDEGDRKGLGVLA
                     GEVKKLPGGVTVPHIGWNTVLSERRASPPLVGSTYFYFDHSYAAHPDDDSVVAGWCEH
                     GSRFAARVERGPILGVQFHPEKSGSQGIALLKEWISDR"
     gene            2176..2961
                     /gene="hisA"
                     /locus_tag="VNP73_00675"
     CDS             2176..2961
                     /gene="hisA"
                     /locus_tag="VNP73_00675"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWL64465.1"
                     /translation="MASSPRGFTVYPAVDISEGRCVRLLQGRFGTETVYSDDPVKVAL
                     GLASAGARWLHIVDLDGAKTGEPANRELVLEVVRLASCPVQAGGGLRTMEDVEEVLAA
                     GANRAVLGTVALEDPAALKKACDRFGPRIVVSLDARGEQLASHGWTVGTGVPVLEAVG
                     IFESAGVSAFVYTDVGRDGTMSGPNVPALLEVAAATQLPVIASGGVGSLDDLRTVAHL
                     YRDGVAGVIVGRAFYEHKFGVVEANLAADEIASGKVEPPLIEP"
     gene            2958..3740
                     /gene="hisF"
                     /locus_tag="VNP73_00680"
     CDS             2958..3740
                     /gene="hisF"
                     /locus_tag="VNP73_00680"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWL64466.1"
                     /translation="MTLAKRIVPCLDVDKGRVVKGVRFVDLVDAGDPAELAARYDAED
                     ADEIVFLDITASHEGRDLILDAVTKTAETVFIPLTVGGGVRSTSDVRRLLSAGADKVA
                     MNTAAVIRPDLVREAADEFGSQAIVVAIDARRKEQACGSEQWDVWIEGGRRPTDLDAV
                     RWARMSCELGAGEILLTSMDRDGTTDGYDLELTKAVADAVEVPVIASGGAGTPEHLAD
                     AVDPERGGAEAALAASVFHFGTFSIQDAKRALLERGLPVRMP"
     gene            3743..4885
                     /locus_tag="VNP73_00685"
     CDS             3743..4885
                     /locus_tag="VNP73_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWL64467.1"
                     /translation="MATFLHVGDGWHSPEMRHEIGEAVFDPVIFIEHEGKRFVVCGPF
                     ETDIFGAREDILDEVVSFETLGADELVKDTSFPEHLILSELAARMLDRIGASSVSVPS
                     TFPTLAADHLRDKQVTVAIDHDGWVMRRRRKAPWEIEGIERAQRAAETAMLTAARMLR
                     DAERAPTGGLRFEGEPLTAELIRESMEAELLMQGTESKQILIHSGDACLSGHDLGTGP
                     IQADQSCIIDCFPHDLTTGTWSDMTRTFVPGQASEELRNLHAHVRKALDVAFDTLKAG
                     SKDAFGHVAEYFHQQGFPTQVHHNGEGMLKEGFMHSLGHGVGLEVHEKPWMGRRSDEL
                     IVGDVVAVEPGLYFPGVGGVRLEDTVLITEDGVEHFTEPFSYDLEP"
     gene            4999..5325
                     /gene="hisI"
                     /locus_tag="VNP73_00690"
     CDS             4999..5325
                     /gene="hisI"
                     /locus_tag="VNP73_00690"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006720181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWL64468.1"
                     /translation="MGPEDLNYDDRGLLPAIVQDASTKDVLMLAWVNAEAMRRTLDTK
                     TTWFWSRSRQEYWNKGATSGNTQKVVEVRYDCDEDVVLMLVDPAGPACHTGETTCFFR
                     SMPEAQ"
     gene            5358..6812
                     /gene="trpE"
                     /locus_tag="VNP73_00695"
     CDS             5358..6812
                     /gene="trpE"
                     /locus_tag="VNP73_00695"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWL64469.1"
                     /translation="MAASQPVVPVWREVLADTQTPVAAFLRLDPKPTGFLLESVEGGE
                     RWARYSFLGGDAFATITARSGRITVEGDAPVEPEQGEAPLAYLRRLLESLHSPPVEGL
                     PPLHGGVVGYIGYDCVRELERLGDPPLDDLELPDLAVVLTRTMVVFDHLLQKAFVIAN
                     VIRPEDPDAAYEDAVARCDDIVRRLAVAQPAPTADLDASAAVDFSSPVDDDEYSKWVE
                     IAREHIYAGDIFQVVPSRRFEAELRGSAFGAYRALRTLNPSPYMYFLRFEGPDGDVEI
                     AGSSPEPLVRVEGDQIVTRPIAGTRPRGASEEEDRATQQELLNDEKERAEHIMLVDLA
                     RNDVGRVARYGSVTVDELMVVELYSHVMHIVSNVTGRLAEGQASFDALTACFPAGTVS
                     GAPKIRAMQIIDSLERTKRGPYGGVVGYFDFSGNLDTCITIRTIVATGGRAYVQAGAG
                     IVADSDPAYEANETRAKAEGMLRAVAAARFVDQE"
     gene            complement(6803..7063)
                     /locus_tag="VNP73_00700"
     CDS             complement(6803..7063)
                     /locus_tag="VNP73_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64470.1"
                     /translation="MAPQSISWLTLEKGTTALGSDGEEIGKVAEVLGDRQKDIFSGVA
                     VRPGLLSSKRFVPAELIENITTDGLTVALPSTEAEARLEPYS"
     gene            7081..7743
                     /locus_tag="VNP73_00705"
     CDS             7081..7743
                     /locus_tag="VNP73_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64471.1"
                     /translation="MIDTRLRACALVMMLLVACAPSESTPTAAPSVPDSPRVLLALIN
                     HHGEQFDVDLRERRAGSQEELAAATYITGHLQLAGYTIRLEDVPVGDLVESSNIMALP
                     PQGDPALVVAAPYDSDGQDASGQALGMFLEVARALNRREPEHHVEFVALGAQLTARGA
                     TTGLSELRRSLDDEGLDPGIFWVTELPSGEVKIEGPQTVIQGPAVQMGARLLELLANR
                     DG"
     gene            7754..8524
                     /gene="trpC"
                     /locus_tag="VNP73_00710"
     CDS             7754..8524
                     /gene="trpC"
                     /locus_tag="VNP73_00710"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWL64472.1"
                     /translation="MPYLSDLLATTRARVDEARAKITEDALEQRVASAEPARGFARSL
                     RGDEVAVIAEIKRATPRAGDLALDLDAGRTASLYRDGGAAAISVLTEPEYFKGSLEDL
                     DAARGPGLPVLRKDFILEPFQIYESRGYGADAVLLIVRTVGPELAELYTLTRALGMDA
                     LVEVHTEEDLDRALELGPDLIGVNHRDLETFELDPDRTAKLTPRIPDGVVVVGLSGVS
                     TRAEVLALKDAGAHAVLVGESVVTATDPVTKLKELRGA"
     gene            8521..9705
                     /gene="trpB"
                     /locus_tag="VNP73_00715"
     CDS             8521..9705
                     /gene="trpB"
                     /locus_tag="VNP73_00715"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWL64473.1"
                     /translation="MTVGEQAVPDEHGRFGEFGGRYVPETLMAALDELERSWETARAD
                     DSFAAELAGLLRTVAGRPTPLYEAARFSERVGTRVLLKREDLAHTGAHKINNTIGQVL
                     LAKRMGKTRIIAETGAGQHGVATATAAAYFGLPCVVYMGAVDTARQHLNVVRMRMLGA
                     EVIPVESGSRTLKDAINEALRDWVTNVESTHYVIGSVVGPHPYPELVASLQSVIGDEA
                     REQCLALIGELPAEVVACVGGGSNAIGLFRAFYRDEDVRLVGVEAAGEGLDRRHGASI
                     SAGRPGVLHGARSMLLQDEQGLIAEAHSISAGLDYPGVGPEHAWLASSGRAIYEAVDD
                     TEALNAFKLLAETEGIVPALEPSHALAYVARQGARDGTVLVGLSGRGDKDVATIAEYL
                     DR"
     gene            9702..>9942
                     /locus_tag="VNP73_00720"
     CDS             9702..>9942
                     /locus_tag="VNP73_00720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWL64474.1"
                     /translation="MTALLEHLRAARSRHKLLVPYVMAGIPDRSGYLDALSAVARHAD
                     AIEVGIPFSDPVMDGPVIAAAGERAIRSGVGPVDAI"
BASE COUNT         1797 a         3017 c         3350 g         1778 t
ORIGIN      
        1 agctccccgc tcgctaccgg atgaacacga acgagtctcc ttacgccccc ccggatgctt
       61 tggtgcgtga agtgtcgaat gagatcgagg cggccgctct caaccggtac cccgaccggg
      121 atgccaaggc cctctatcga gcgatctcgg atcacatcga ccacccggtc gagggcctgt
      181 gggtggccaa cggctcgaac gaagtcttca tgcacctgtt cctcggtttc gggggagccg
      241 ggcgaacgac ccttttgttc gagccgacct actcggtgca ctcgttgatc ccgcgtatcg
      301 cctccacgaa cgtcgtcccg atcgaccgag gtgagaactt ccacatagac gtcgatgagg
      361 cactcgacgc gatagtggag gggcgacccg atgtcgtcat cgtgtgctcg ccgaacaatc
      421 caacaggcaa ctgcgaatcg ataggcacca tcgaggcaat actcgaagcg gcccctggtc
      481 tggtggtcgt cgacgaggcg tacggagagt tcgcgcatcc gggtgagacg gtgaggccct
      541 tgctgaaatc gcaccgcaat ctcgttgtga cgaagacatt ctcgaaagcg tggcgcctgg
      601 ccggtgtgcg tatcggatac atgatggccg atccgcaatt ggtaaacgag cttcagcggg
      661 tcaaattgcc gtaccacttg tccacgatca cgcaggcggt tggggtcgcg gcgatccgtc
      721 actcggacga aacattgaga ctcgtcgatg ccctcatcac cgagcgagat cgccttgcta
      781 tgggactcga agcgatggga ctcaaggtgt atccgtcgcg cgcgaacttc gtgctattcg
      841 agatagagga ttcggagcgc gtgtggaaag aactattgga acgggatgtc ctgatccgca
      901 actactccgg ggagcgcggc ctcgaggatt gcctccgggt aaccgcgggc ttgccggagg
      961 agacggacgt cttcctgtcg acgctggaag agatcctcga tgactgaacg ccgagccgtg
     1021 gtcgaaagaa cgaccagtga gaccaagatc cgcgtcgaac tcgacctcga cggcggagcg
     1081 cccagcatct ctacgggagt gccgttcttc gaccacatgc tggaccagct ggcgcgccac
     1141 gcgcgcgtcg ggctcgtcgt ggaggccgaa ggggatctag agatcgacgc gcatcacacg
     1201 gtcgaagacg tcggtatcgc tctcggagag gccctggcga aagcactggg cgacaagcgc
     1261 ggcatccaca ggtatggaga cgcgctcgtg ccgatggaag aggccttggc gcaggtcgcg
     1321 ctcgacatct cgggccgtcc gctgctgctc tacaccgcgg acttcccggc ggagtcgatc
     1381 ggccagtacg acacggtcct gacggaggag ttcctgcaag ctctgagccg gaccgcgggg
     1441 ctgacgctcc acgtgcgcct gctcgaaggt cgcaatgctc accacgcggt ggaggcgatc
     1501 ttcaaggctc tggcacgcgc cctgggggcc gcggtctcta ccgacccgcg tgccggcgac
     1561 gacatcccgt ccacgaaggg cacgctgtga aggtcgcagt ccttgaccac ggcatgggca
     1621 acctccgtag cgtggcgaag gcgatcgaag ctgcgggagc cgatgtgacc gtgaccggcg
     1681 aggccgcgga cgtccaacga tccgatgccc tctgcgttcc gggacaaggc atcttcggtc
     1741 gttgcatgga gaacctgacc gggaccggga tggacgacct gatccgctcc tggatcgact
     1801 caagccgtcc ttatcttggc atctgcctcg ggatgcaagt gctgttcgac cggagcgacg
     1861 aaggcgatcg caagggtctc ggtgtgctgg cgggggaggt caagaagttg ccgggcggtg
     1921 taacggttcc tcacatcggc tggaacaccg tgctttcgga gcgccgggcg agcccgccgc
     1981 tcgttggaag cacctacttc tacttcgatc actcgtatgc ggcgcatccg gacgacgact
     2041 ccgtcgtggc cggctggtgc gagcacgggt cgagattcgc ggcacgtgtc gaacgcggcc
     2101 ctatcctcgg cgtccagttt caccccgaga agagtggttc tcaggggatc gcactactca
     2161 aggagtggat ctcagatcgc tagctcacca cgaggcttca ccgtctaccc cgccgttgat
     2221 atctccgaag gtcgctgtgt caggttgttg cagggcagat tcgggacgga gacggtgtac
     2281 tccgacgatc cggtcaaagt cgcgctcgga ctcgcgtcgg caggggcacg ctggcttcac
     2341 atcgtcgatc tcgacggtgc caagaccgga gagcccgcca accgcgagtt ggtgctggaa
     2401 gtcgtgcggc ttgcctcgtg tcccgtgcaa gccgggggcg gcttgcgaac gatggaggat
     2461 gttgaggagg ttcttgccgc aggcgcgaac cgggccgtcc tcggaacggt cgcactcgaa
     2521 gatccggcgg cgctgaagaa ggcatgtgat cgtttcgggc cgcgcatcgt ggtgtcgctc
     2581 gacgctcgcg gcgagcagct cgccagtcac ggatggacgg tgggtaccgg cgtgccggtt
     2641 ctcgaagcgg tcggcatctt cgaatctgcc ggtgtgtcgg cgttcgtcta caccgatgtg
     2701 ggccgggacg ggacgatgtc gggaccgaac gtccccgctc tgttggaggt ggcggccgca
     2761 acccaattgc cggtcatcgc gtccggagga gtcggttcct tagacgacct gaggaccgtc
     2821 gctcatctgt accgagacgg ggtcgccggt gtcatcgtcg gccgtgcttt ctacgagcac
     2881 aagttcggcg ttgtcgaagc gaaccttgcc gccgacgaga tcgcgtccgg caaggtggag
     2941 ccaccactga tcgaaccatg acactggcga agaggatcgt gccctgcctc gacgtcgaca
     3001 agggacgggt agtgaagggc gttcggttcg tcgacctcgt agacgccggg gatcctgccg
     3061 agcttgcggc gcgctacgac gcagaggatg ccgacgagat cgttttcctc gacatcaccg
     3121 cgtcgcacga ggggagagat ctcatcctcg acgccgtaac gaagaccgcc gaaacggttt
     3181 tcatcccctt gaccgttgga gggggggtga ggtcgacgtc cgacgtccgc aggctcctgt
     3241 ctgcaggagc cgacaaggtg gcgatgaaca ccgctgccgt gatccgtccg gatctggtgc
     3301 gcgaggccgc ggacgagttc gggtcacaag cgatcgtcgt cgcgatcgac gcgcgccgca
     3361 aagagcaggc gtgcgggtcc gagcagtggg acgtctggat cgaggggggc cgacgtccga
     3421 ccgatctcga cgccgtgagg tgggctcgta tgtcgtgtga gctgggagcc ggagagatcc
     3481 tcctgacgtc gatggatcgg gacgggacca ccgacggcta tgacctcgaa ttgacgaagg
     3541 ccgtcgccga tgcagtcgag gtgcccgtga tcgcctcggg tggcgcgggt acaccagaac
     3601 atcttgcgga cgcggtcgac cctgaacgcg ggggagccga ggcagcgctc gcggcctccg
     3661 tgttccactt cggaacgttt tcgatacagg acgctaagcg cgcgcttttg gagcgcggcc
     3721 tccccgttag gatgccgtga cgatggcgac attccttcac gtcggcgacg gctggcattc
     3781 ccccgagatg cgacacgaga tcggcgaagc cgtattcgat cccgtgatct tcatcgagca
     3841 cgagggcaag agattcgtcg tgtgcggccc cttcgagacc gacatcttcg gggcgaggga
     3901 ggacatcctg gacgaggtcg tgtccttcga gactctcggg gcggacgaac tcgtgaaaga
     3961 cacgtcgttc cccgaacatc tgatccttag cgagcttgct gccaggatgc tcgaccggat
     4021 cggcgcctcc agcgtcagcg ttccgtcaac gttccccacc ctggcggccg atcatctacg
     4081 cgacaagcag gtcacggtcg ccatcgacca cgacgggtgg gtgatgaggc gcaggcgaaa
     4141 agctccgtgg gagatcgaag gtatcgagcg tgcccagcgc gcggccgaga cggcgatgtt
     4201 gacggcggcg cggatgctcc gggacgccga gcgtgcacca accggtggcc tgcgtttcga
     4261 aggggagccg ctcaccgccg aactcatccg tgagtcgatg gaggccgagc tcctgatgca
     4321 gggaacggag tcgaagcaga tcctcatcca ttccggggat gcctgcctgt cgggtcacga
     4381 cctcggcacc gggccgatac aggccgatca gtcctgcatc atcgattgct tcccgcacga
     4441 cctgacgaca ggtacgtggt cggacatgac ccgtacgttt gttccggggc aagcgtccga
     4501 ggagttgcgc aacttgcacg ctcacgtaag gaaggccctc gatgtggcgt tcgacacgtt
     4561 gaaggccggt tccaaggatg cattcggaca cgtggcggag tacttccacc aacagggctt
     4621 tccgacgcag gtccatcaca acggcgaggg gatgctgaag gaagggttca tgcactcctt
     4681 gggccacggc gtgggtctcg aggtgcatga gaagccgtgg atgggtcgtc gatccgacga
     4741 actgatcgtc ggggacgtgg tcgccgtcga acccggtctc tactttcccg gagtgggtgg
     4801 ggtgaggctc gaagacacgg tcctgatcac cgaggacgga gtcgaacact tcacggaacc
     4861 cttctcctac gacctggagc cctagcctcg tcttccgcgc gcagaaggga caatgagcga
     4921 tcgtctcgac gatcgcgccg tccgaaagga agggctgccc gcggcagcgg gcatacctga
     4981 gggaggacaa tgggggtcat gggacccgaa gacctcaatt acgacgaccg ggggctgttg
     5041 ccggcgatcg tgcaggacgc gtcgacgaaa gatgtcctga tgctggcatg ggtcaacgcc
     5101 gaagcgatgc gccggacttt ggataccaag acgacgtggt tctggagtag gtcccgccag
     5161 gagtattgga acaaaggcgc gacctccggc aacacgcaga aggtcgtcga ggtccgctac
     5221 gactgcgatg aagatgtcgt actcatgctc gtggatccgg ccggacccgc gtgccacacg
     5281 ggagagacga cctgcttctt ccggagcatg ccggaggcgc agtgacctgc cgccccgatc
     5341 cggctacctt cagggcgatg gcggcgtcgc aacccgtggt tccagtctgg cgcgaggtcc
     5401 tcgccgacac acaaacgccc gtagcggctt tcctgaggct cgatcccaag ccgaccgggt
     5461 tcctcctgga gagtgtcgag ggcggcgagc ggtgggcccg ttactcgttc ctcggcggcg
     5521 acgcgttcgc aacgatcacc gctcgttcgg ggcgcatcac ggtcgagggg gacgcgccgg
     5581 tagaaccgga gcagggtgaa gctccacttg cgtaccttcg acgtctgttg gagtcactgc
     5641 attcgccgcc cgtcgagggc ttgcccccac tgcacggagg cgtggtgggc tacatcggat
     5701 acgactgcgt gcgggagctt gaacggctcg gtgatcctcc tctcgacgac ctcgagcttc
     5761 cggatcttgc agtcgtgctc acgcgcacta tggtcgtttt cgatcaccta ttgcagaagg
     5821 cgttcgtcat cgccaacgtt atccgtccag aggacccgga tgcggcctac gaggacgccg
     5881 tcgctagatg cgacgacata gttcgccggc tggcggtggc ccagccggca cctaccgccg
     5941 atctcgatgc ctcggcggcc gtggacttca gctcgcccgt ggatgacgac gagtactcga
     6001 agtgggtcga gatagcgcgc gagcacatat acgcgggcga catctttcaa gtggttccgt
     6061 cccggcgctt cgaagccgag cttcgcggct cggctttcgg cgcgtaccgg gcgctacgaa
     6121 cgctcaaccc cagtccctac atgtatttcc ttcggttcga aggccccgac ggggatgtgg
     6181 agatcgccgg ctcttcgccc gaaccgctcg ttcgcgtcga aggggaccag atcgtgaccc
     6241 gtccgatcgc cggcacgaga ccgcgcggag cttccgagga agaggaccgg gccacccaac
     6301 aggagcttct gaatgacgag aaagaacgcg ccgagcacat catgctcgtc gacctggcac
     6361 gcaacgacgt cggcagggtg gcacgctacg gatccgtgac cgtcgacgaa ctgatggtgg
     6421 tcgagctcta ttcgcacgtt atgcacatcg tgtccaacgt caccggacgg ctcgccgaag
     6481 ggcaggcctc gttcgatgcc ctgaccgcat gtttccccgc ggggactgtg tcgggtgcgc
     6541 cgaagatcag agcgatgcag atcatcgact cgctcgaacg aacgaagcgc ggtccctacg
     6601 gaggggtcgt gggctacttc gacttctcgg ggaatctgga tacgtgcatc acgatccgaa
     6661 cgatcgtggc aacaggaggt cgggcgtacg tgcaagcggg ggccgggatc gttgccgatt
     6721 cagatcccgc ctacgaggcg aacgagacgc gcgctaaagc cgaggggatg ttgcgggccg
     6781 ttgcagccgc ccgcttcgtg gatcaggaat agggctcgag gcgagcttcc gcttcggtgg
     6841 acggaagcgc gacggtcaat ccgtccgtgg tgatgttctc gataagctcg gcagggacga
     6901 accgcttgct cgacaggagt cccggccgga ccgcgacccc cgagaagatg tctttctggc
     6961 gatcacccag aacctccgcg accttcccga tctcctcacc gtccgagccc aaggctgtag
     7021 tgcctttctc gagcgtgagc catgagatcg attgcggagc catcaacgga tactaagtgc
     7081 gtgatcgata cccgactccg cgcatgtgca ctcgtaatga tgctgctcgt cgcctgcgcg
     7141 ccgagcgaaa gcacgccgac tgcggccccc agcgttccgg attcaccacg ggtcctgctt
     7201 gcattgatca accatcacgg ggagcagttc gacgtcgatc tacgggagcg acgggcgggc
     7261 tcgcaggaag agctcgcggc cgcgacgtac atcaccggtc acctccaact ggcgggctac
     7321 acgatccgcc tcgaagacgt gccggtcggt gatctggtcg agtcctccaa catcatggcc
     7381 ctccctcccc agggagaccc ggcgctcgtc gtggctgctc cctacgactc cgacggccag
     7441 gacgcttccg gtcaggcgct cgggatgttc ctcgaggttg cacgcgcgct aaacagacgc
     7501 gagccggagc atcacgtcga gttcgttgca ctcggggcgc agctaaccgc ccggggcgca
     7561 actaccggtc tcagcgaact ccgcagatcc ctcgatgacg aaggactgga cccggggatc
     7621 ttctgggtta cggagcttcc ctcgggtgag gtgaagatcg agggtcccca gaccgttatc
     7681 caaggaccgg cggtgcagat gggggcgcgc ttgttggaat tgctcgcgaa tagggacggc
     7741 tagggtctcg aagatgccgt acctgagcga tcttctggcc acgacccgag ctcgcgttga
     7801 cgaggcgcga gcgaagatca ctgaagacgc actcgaacaa agagtggctt cggccgagcc
     7861 cgcccgggga ttcgcacggt cgcttcgcgg cgacgaggtg gcggtgatag cggagatcaa
     7921 gagggcgacc ccgagggcgg gagatctcgc tctggatctc gatgccggcc gaacggcatc
     7981 tctgtatcgg gacggggggg ccgccgcgat ctcggtcctt accgagccgg agtacttcaa
     8041 gggatctctg gaggatctgg acgcggctcg cggaccgggc ttgcccgttc tgcgcaagga
     8101 cttcatcctc gagcctttcc agatctacga gtcgaggggg tatggggccg atgccgtgtt
     8161 gttgatcgtg cgcacggtgg gccccgagct cgcggagcta tacacgctga cccgggctct
     8221 ggggatggac gcgttggtcg aggtacacac tgaagaggat ctcgatcgtg cgctcgagct
     8281 ggggcccgat ctcatcggag tgaaccacag ggatctcgag accttcgagc tcgatccgga
     8341 ccgaacggcg aagttgacac cgcgtatccc cgatggagtc gttgtcgttg gtttgtccgg
     8401 ggtctccacg cgcgccgagg tattggcctt gaaggatgca ggtgcgcatg ccgttctggt
     8461 gggggagagc gtggtaaccg cgaccgatcc cgtgacgaag ttgaaggagc tgagaggcgc
     8521 gtgacggtag gcgaacaagc ggttcccgac gaacatggac gcttcggcga gttcggcgga
     8581 cgttacgtcc ccgagacgct catggcggct cttgatgagc tggaacggtc gtgggaaacg
     8641 gcccgggccg acgactcttt cgcagcggag ctcgcggggc tgctgcgtac cgttgcagga
     8701 cgcccgacgc ccctgtacga ggccgcgagg ttctccgagc gcgtagggac gcgggttctg
     8761 ctcaagcgtg aggacctcgc ccataccggg gcacacaaga tcaacaacac gatcggccag
     8821 gtcttgcttg ccaaacggat gggcaagacg cggatcatcg ccgaaacggg agccggccag
     8881 cacggcgttg cgaccgctac cgccgccgcc tacttcggac tcccttgcgt tgtctacatg
     8941 ggcgcggtcg acaccgcccg ccagcacctg aacgtagtgc ggatgcggat gctgggggct
     9001 gaggtcatcc ccgtcgagtc cgggtcacgc accttgaagg acgcgatcaa cgaggcgttg
     9061 agggactggg tcacgaacgt ggagtcgacg cattacgtga tcggctcggt tgttggaccg
     9121 catccttatc cagagctggt cgcttcactc caatcggtca tcggagacga ggcacgcgaa
     9181 caatgcttgg ccttgatcgg cgagctcccc gctgaagttg tcgcgtgtgt cgggggtggt
     9241 tcgaatgcga tcggtctctt ccgggcgttc tatcgggacg aggacgtccg tctcgtaggg
     9301 gtcgaggcgg caggcgaggg actcgatcgg cgtcacggcg cctcgatatc ggcgggtcga
     9361 ccgggcgttc tccacggcgc gcgcagcatg ttgctgcagg acgagcaagg ccttatcgcg
     9421 gaagcgcact cgatatcggc aggtctcgac tatccgggtg tcggaccgga gcacgcatgg
     9481 cttgcctcga gcggtcgtgc catctacgag gcggtcgacg acaccgaagc gttgaacgcg
     9541 ttcaaacttc tggccgagac ggaagggatc gttccggcgc tcgaaccgtc ccacgcactc
     9601 gcatacgtgg cgcggcaggg tgctcgagac gggactgtat tggtcgggct ttcgggacgg
     9661 ggcgacaaag acgtcgcgac gatcgccgaa tacctcgatc gatgacggcg ctactcgagc
     9721 atctacgggc ggcgcggtcg cgccacaagc ttcttgttcc gtacgtcatg gccggtatcc
     9781 cagaccgctc gggttacctc gacgcgctgt cggccgtcgc tcggcatgcc gacgcgatcg
     9841 aggtcggcat ccccttctcc gatccggtca tggatgggcc cgtcatcgcg gctgccgggg
     9901 agagagctat ccggtcgggg gtcggcccgg tcgacgcgat cg
//
LOCUS       DATMED010000028         3326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3864433, whole genome shotgun sequence.
ACCESSION   DATMED010000028 DATMED010000000
VERSION     DATMED010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3864433"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VNP73_00725"
     CDS             <1..836
                     /locus_tag="VNP73_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWL64475.1"
                     /translation="WDFIVVALVGGVLSSAIVLTVTVYLSITSFRKGWDLDSVGAPLI
                     TAMGDVVTLPCLFAASFLVEIRGFTVIAGALGVVLALVALARGWTTAKQISRRIVRES
                     FPVLCIAIVLDVLAGTVVEPRMEDVFLPYPALLILIPGFLENTGALGGILASRLGSKL
                     HLGAVSARARPDPAALLDGSIILVLGLTVYTIAGVTTLILSTVIGAEYPGALRFLGIT
                     LFGGALATMVAALIGYYAAATTYRFGFDPDNHTIPLVTSGMDLLGVICLVIALAVFGV
                     G"
     gene            847..2040
                     /locus_tag="VNP73_00730"
     CDS             847..2040
                     /locus_tag="VNP73_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWL64476.1"
                     /translation="MESGEGRHTVKGLLAEIKDESELMVDLAYAAVYFSDSDLAREVA
                     RLEERMFAQLRALRKLAILAARSPEDAEHMAGVLEIASAVEKIADAAEDIAHVVGLGI
                     PDDLKVDLRHADEIVSRIKVREGSAGIGRTLRELALPVEIGMWVIATRRGGEWNLDPD
                     ADYCIAENDVLLAKGPEDGVALAREIMGAPPLPEAPPGPSIPLSDLDRAVDLLVEMKN
                     SAEVAVGLAYSALLFNDQALAAEVSNLEARSDAVHDELESWVLRSSQEARDTDELRGL
                     LHLAWASEMIFDSAREMTRLVEQGEELHPVIAAALVESDEVGHETVVGSGSQADGKSI
                     KELSIETETGMFVLAVQRGRRWTYRPKPRFVLQADDRIIAIGPPEGGEELDEMVGMQR
                     EMVEA"
     gene            2147..2431
                     /locus_tag="VNP73_00735"
     CDS             2147..2431
                     /locus_tag="VNP73_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HWL64477.1"
                     /translation="MSETDEVQPDGSTPQKATLFQTILAAIVGMIAVRVATYLVTTMW
                     RLVTREDPPQIDQRAPVGKKAAWVGLVGAASGAARQAARDLVKPPGDGPA"
     gene            complement(2428..>3326)
                     /locus_tag="VNP73_00740"
     CDS             complement(2428..>3326)
                     /locus_tag="VNP73_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64478.1"
                     /translation="EETMGGIAVGLLFTSFLNGLRHGVDFDHVAAITDIATTQTDKKS
                     SMILSTAYALGHAFVVLCLGVAAVVVGAHLPSPIDSFMSKVIGATLLVLGVYVLYSIV
                     RYRRNVRLQSRWMLIARGVRAVWTRLYDRRSSVTVIEHEHDHGHSVGHDHDHEDLSRT
                     GPGPSTSVALKTKSHTHVHRHLGTVPADPFASYSKLGAFGIGMLHGVGAETPTQLLLF
                     VTAAGIGSKAFGIGVVVSFLVGLFASNTAVAVASSFGLSRGRRAPYVYMGLAAVTAAF
                     SLFLGTAYLLAREDWIALVIGV"
BASE COUNT          625 a         1016 c         1080 g          605 t
ORIGIN      
        1 tgtgggactt catagtcgtt gcgctcgtcg ggggggtgct gtcctccgcc atcgtgctca
       61 cggtgaccgt ctacctctcg atcacctcgt tccgcaaagg ctgggatctc gactcggtag
      121 gcgcacctct gatcacggcc atgggagatg tggtgacgct gccgtgtctg ttcgccgcgt
      181 cgttcctggt cgagatccgc ggcttcaccg tcatagccgg cgccctgggg gtcgtcctgg
      241 cactggtcgc gttggcgcgt ggctggacca ccgccaagca gatatcccgg cggatcgtcc
      301 gtgagagctt tccggtcctc tgcatcgcga tcgttctcga tgtcttggcc ggcacggtcg
      361 tggagccgcg gatggaagat gtcttccttc cgtatccggc cctactcatc ttgatccccg
      421 gtttcttgga gaacacgggc gcgctcgggg gcatcctcgc ctcgcgtttg ggctcgaagc
      481 tgcacctcgg tgccgtcagt gcgcgcgccc ggccggaccc cgccgccctt ctggacggaa
      541 gcatcatctt ggtgctggga ctgaccgtgt acacgatcgc cggagtcact accttgatct
      601 tgtccacggt tatcggagcc gagtatcccg gcgccctgcg cttcctcggc atcaccttgt
      661 tcggaggcgc cctggcaacg atggtggctg cgttgatcgg ctactacgcg gcagccacga
      721 cgtaccggtt cgggttcgat cccgacaacc acaccatccc gctcgtgacg agtgggatgg
      781 atcttctggg tgtcatctgc ctcgtaatag ctctggccgt cttcggagta ggttgaaagg
      841 agaacgatgg aatctggtga gggccgacat acggtcaaag ggctcctggc cgagatcaaa
      901 gacgagtcgg agctcatggt cgacctggcg tacgccgcgg tctatttctc cgacagcgac
      961 ctcgctcgcg aggtagcgcg gctagaggag cggatgttcg ctcagcttcg cgccctgcgg
     1021 aagctcgcga tcctcgcggc gagaagcccc gaagacgcgg aacacatggc cggcgttctc
     1081 gagatcgcat ctgcggtcga gaagatcgcg gatgccgccg aagacatcgc gcacgtggtg
     1141 ggtctgggga tccccgacga cctgaaggtc gatctgcgcc acgccgacga gatcgtctcc
     1201 cgcataaaag tgcgcgaggg atccgccggg ataggaagga cgctcaggga gctcgcactc
     1261 ccggtagaga tcgggatgtg ggtgatcgcc acccgacgtg gaggcgaatg gaacctggac
     1321 cccgatgccg actactgcat cgcggagaac gacgtgctcc tggccaaggg ccccgaagac
     1381 ggggttgctc tggcgcgcga gatcatgggt gcccctccct tacccgaggc accaccaggc
     1441 ccctccatcc ccctatccga tctggatcga gcggtggatc tcctcgtcga gatgaagaac
     1501 tcggccgaag tagcggtggg actggcttat tcagctctcc tgttcaacga tcaggcattg
     1561 gccgccgagg tcagcaacct cgaggcgcgc tcggacgcgg tgcacgacga actcgagtcg
     1621 tgggtcctta ggagctcgca ggaggcgcgg gatacggacg agctgcgcgg cctcctccac
     1681 ctcgcctggg catccgagat gatcttcgac tcggcccgtg agatgacacg actcgtcgaa
     1741 cagggtgagg aactgcatcc ggttatcgcg gcggcattgg tcgaatccga cgaggtcgga
     1801 cacgagaccg tcgtgggatc tggttctcaa gctgatggaa agtcgatcaa ggagctttcg
     1861 atcgaaaccg agacggggat gttcgtcctc gccgtgcaac gcggacgcag gtggacgtac
     1921 aggccgaaac ccagattcgt ccttcaggca gacgaccgca tcatcgcgat cggaccaccg
     1981 gaaggcggcg aagagctaga cgagatggtc ggcatgcaac gagagatggt cgaggcgtag
     2041 gcaggtgatg ggctcggcca tcacccctcc ggagcggtga gatcccggca ccgccgggaa
     2101 atcatccgcg taggagaatt gagagttcag ggcatactct cgggacatga gcgagacgga
     2161 cgaggtccaa ccggacggca gcaccccgca gaaggccacc ctgttccaaa cgatcctggc
     2221 tgcgatcgtg gggatgatcg cggtgcgggt tgcgacctat ctcgttacga cgatgtggcg
     2281 actcgtcacg agggaggacc ctccgcagat cgaccagcgc gcgccggtcg gcaagaaggc
     2341 tgcctgggtc ggattggtcg gggccgcttc gggggccgcg cggcaagcag ctcgggatct
     2401 cgtaaagcct cctggagacg gtccggctta gacgccgatc accaacgcga tccagtcctc
     2461 gcgcgccagt aggtaggcgg tcccaaggaa gagactgaaa gccgcggtca cagcagcaag
     2521 ccccatgtag acgtagggag ctcgtcgtcc gcgcgataaa ccgaaagagg acgcaaccgc
     2581 caccgctgtg ttcgaggcga acagaccgac gaggaaagac acgaccactc cgatcccgaa
     2641 agctttgctt ccgatgccgg ccgcggtcac gaacagtagg agctgcgtcg gtgtttccgc
     2701 tcccacgcca tggagcatcc caatgccgaa cgctccgagc ttgctgtagc tcgcgaacgg
     2761 atcggcagga acggtcccga ggtgccggtg aacgtgcgta tggctcttgg tcttcaacgc
     2821 caccgaggtc gaaggcccag gcccggtccg cgacagatcc tcgtgatcgt ggtcgtgtcc
     2881 gacggagtgt ccgtgatcgt gttcgtgttc gatgaccgtg acgctcgagc gacggtcgta
     2941 aagacgggtc cacacggccc gcactcctcg tgcgatgagc atccatctgc tttggaggcg
     3001 aacgttcctt ctatagcgaa cgatcgaata gaggacgtat acgccgagta cgagcagcgt
     3061 tgcaccgatc accttgctca tgaacgagtc gatcggcgac ggcagatgag cccccacgac
     3121 aaccgccgcc actccgaggc agagaacgac gaatgcgtga ccgagtgcgt acgcggtcga
     3181 gaggatcatc gagctcttct tgtcggtctg agtggtcgct atatcggtga tggccgccac
     3241 gtgatcgaag tccaccccat ggcggagacc gttcaagaag ctggtgaaga gaagccctac
     3301 cgcgatgcca cccatcgttt cctcct
//
LOCUS       DATMED010000029         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4631351, whole genome shotgun sequence.
ACCESSION   DATMED010000029 DATMED010000000
VERSION     DATMED010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631351"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VNP73_00745"
     CDS             <1..1277
                     /locus_tag="VNP73_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWL64479.1"
                     /translation="IAYKHVREEAANPSSLNRDVPPALDSITRKALAKNAENRYTDAS
                     EMHADLQRFLSGQQVHATPLMADTTMVADRVTGTRVIDEIPYEEEPRGRPGLYVLIAL
                     LILGLFAALAWFVATRVINPGDPVKVPKLVGLDIDAAEARLDDLGLEADVEEKRSPKP
                     EGEVLKQDPEQGETLREGDAVALVVSLGRAQIDVPDVVGLQLEEARDELEDARLKVGR
                     VDQEASEDVPEGEVIDQSPSAGTTVDARSPVALVVSSGPEEVTVPDVVGLSEAEAVDV
                     IQDARLRVQINRGPSDEFEEGIVAGQDPAASTEVEEGTTVVILVSEGPEARPMPDVTG
                     QDADDAEATLESDYGLDVSQQDADPSQCGAAPPGTVCDQDPAPGTEVSPGDSAVLYVQ
                     PGQAELPGPGWAAAWFFGLAGLERLRRRDEVL"
     gene            complement(1242..1820)
                     /locus_tag="VNP73_00750"
     CDS             complement(1242..1820)
                     /locus_tag="VNP73_00750"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWL64480.1"
                     /translation="MRLLLIDNFDSFVYNLAQAFGAIGVEPVVVRNDVNLADLKKIEP
                     DAVVVSPGPGTPETAGVSIEAVEYFGGRVPVLGVCLGHQCIGAAFGGRIDRAPVGPVH
                     GKTSQISHDGKGVFAGLSSPFVATRYHSLCVTEGEVPDELEVTASSEDGVIMGVRHRT
                     LPIEGVQFHPESVLTVDGPRLLENFVASAESL"
     gene            complement(1798..>2127)
                     /locus_tag="VNP73_00755"
     CDS             complement(1798..>2127)
                     /locus_tag="VNP73_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64481.1"
                     /translation="PVIALGYAFTFILGYASALGVRVLGRPQLVAAAAASLVAEIAVI
                     AATWDRYRFVGTRSQWSAGDAYPLFGVSPEGPVRLISVLGPIFAVALAAGVWIAWRGS
                     RAAAADR"
BASE COUNT          438 a          700 c          678 g          311 t
ORIGIN      
        1 cgatcgcgta caagcacgta cgcgaggaag cggccaaccc gtcctcactg aaccgggacg
       61 tgccgccggc gctcgactcg atcacccgca aggcgctcgc gaagaatgcc gagaaccgct
      121 acaccgatgc ctccgagatg catgccgatc tccaacggtt cctgtcggga caacaggtgc
      181 acgcgacgcc gctcatggcc gacaccacga tggttgccga ccgggttacg ggcactcgcg
      241 tgatcgacga gatcccctac gaggaagaac cccgggggcg acccggccta tacgtcctga
      301 tcgcgctcct gatcctcggg ctgttcgcag ccctggcgtg gttcgttgcg acacgcgtca
      361 taaaccccgg cgatccggtg aaggttccca agctcgtcgg tctggacatc gatgccgccg
      421 aggcaaggct cgacgacctg gggctcgaag ccgacgtgga ggagaaacgg agtccaaagc
      481 ccgagggcga agtcctcaag caagatcccg agcaaggaga gaccttgcgt gagggagatg
      541 ccgttgcact cgtcgtctcg ttgggccgtg cacagatcga tgtacccgac gtggtcggcc
      601 tgcagctgga ggaggcccgc gacgagctgg aggacgcgcg gctcaaggtg ggacgggtcg
      661 accaggaagc gagcgaggac gtacccgagg gagaagtcat cgatcaatcg ccatcggccg
      721 gcactacggt cgacgcgcgt agcccggtcg cgctcgttgt gtccagcggc cccgaagagg
      781 taaccgttcc cgatgtcgtg ggcttgagcg aagcggaagc cgtcgacgtg atccaggacg
      841 ctcgattgcg agtccagatc aaccggggcc cgagcgacga attcgaagaa ggcatcgtgg
      901 cagggcaaga tccggccgcg agcaccgagg ttgaggaggg aacgacggtc gtcatcctcg
      961 tgtccgaagg gccggaagcc aggcccatgc ccgacgtaac tggtcaagat gcggacgatg
     1021 cggaggcgac gctcgagtcc gactacggac tggatgtgag ccagcaggac gccgatccgt
     1081 cccagtgtgg ggccgctccg ccgggaacgg tgtgcgacca ggaccccgcg cccggaaccg
     1141 aggtcagtcc gggagacagt gcggttctgt acgtgcaacc cggccaggca gagctccccg
     1201 gccccgggtg ggcggccgcg tggttcttcg gcctcgccgg cctagagaga ctccgccgac
     1261 gcgacgaagt tctctagtaa ccgaggtccg tcgaccgtca ggacggactc aggatggaac
     1321 tgcacacctt cgatgggcaa ggtcctgtgc ctcaccccca tgatgacgcc gtcctcacta
     1381 gacgccgtca cttccaactc gtcgggaacc tctccttccg tcacacagag cgagtgataa
     1441 cgcgttgcga cgaaaggact ggataacccg gcgaaaactc ccttcccatc gtgactgatc
     1501 tgtgacgtct tcccatggac cggcccgacc ggcgcgcgat cgatccggcc cccgaaagcc
     1561 gcgccgatgc actggtgacc cagacaaacg ccgagcaccg gcacgcggcc tccgaaatac
     1621 tcgaccgcct cgatcgatac gcccgcggtc tcgggagtcc ccgggcccgg cgacacaacg
     1681 acagcatccg gttcgatctt cttgagatcg gcgagattga cgtcattgcg aacgacgacg
     1741 ggttctacgc cgatcgcacc gaaagcctgc gcgaggttgt agacgaagga atcgaagtta
     1801 tcgatcagca gcagccgcac ggctccctct ccacgcgatc cacacaccgg ctgcgagcgc
     1861 cactgcgaag atcggcccga gcacactgat gagccgcacc gggccttcgg gtgacactcc
     1921 gaacaatgga tatgcatcgc ccgcgctcca ctgcgaacgc gtccccacga agcggtagcg
     1981 atcccacgtg gccgctatga cggctatctc tgccaccaga gaggcagccg cagcagcaac
     2041 cagctgggga cgtccaagaa ccctcactcc cagagcggac gcgtaaccca gtatgaaggt
     2101 gaaggcatac ccgagcgcga tcaccgg
//
LOCUS       DATMED010000030         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3475619, whole genome shotgun sequence.
ACCESSION   DATMED010000030 DATMED010000000
VERSION     DATMED010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475619"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VNP73_00760"
     CDS             complement(<1..657)
                     /locus_tag="VNP73_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64482.1"
                     /translation="MIQTDGVAAPLAPFSAAARVAGAVIAMAYLLGLVPGPLVTVVGG
                     LALITFGRALLLPLLPSARAVLSLAVIAGAVGIGALRWGTLDLAELRGVQAVLGPTVT
                     VGPPAAAIACGIALGAAVVVLAVWFLEPAPGDRTDHVWGGFEVALVALALATIFIDPA
                     RSVLHGAGLAIALLEIVRWAGVTAVTAAVALGIARLLRDTGDFVRWVVLGVAGAAVAV
                     A"
     gene            complement(654..1130)
                     /locus_tag="VNP73_00765"
     CDS             complement(654..1130)
                     /locus_tag="VNP73_00765"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HWL64483.1"
                     /translation="MDVTGRSFDPTRMPPPPAGPSVDYRLARRAALGSLTRGSLDTAD
                     LCDAHPELLRAGKNIGEPVQDRCPVCSHDALRWVRYVYGDELRHNNGKVVYPLEWLEE
                     LTTKYDQFTCYIVEVCIDCAWNHLVRAFYAGRLYGTNGTTPGTGSRSSNGRNRRRA"
     gene            1284..1916
                     /locus_tag="VNP73_00770"
     CDS             1284..1916
                     /locus_tag="VNP73_00770"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWL64484.1"
                     /translation="MRQPGMLDLAVLGLLKERAMHGYELRKQLGAMLGPFWQVSWGSL
                     YPTLRRLAKFGAVEKLDAPLRKTTRAAKTTGKRSARATKSKSKSTTLSSGRRKNVYQI
                     TAKGEGMFVAMLEETAAATDAEHFTLKLAFFRYLQPEMRLALLERRRAYLQEKLAQFK
                     TNIRDYKERVDSYTLSLQNHGMAATQSDIDWIDELITAERALDRVRPTKA"
     gene            1925..>2357
                     /locus_tag="VNP73_00775"
     CDS             1925..>2357
                     /locus_tag="VNP73_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWL64485.1"
                     /translation="MAKVRVGIVGAGNCASSLVQGVEYYAKARADERVPGLMHVDVGA
                     YHVSDIEFSCAFDVDANKVGLDLADAIWVEPNNTIKFSDVPSLDLKVERGHTYDGLGS
                     FYKDVVTESELPPVDVAKILRDTHTDVLVNYLPVGSEQAARF"
BASE COUNT          478 a          766 c          763 g          350 t
ORIGIN      
        1 agcgacggcc acagcagcac cggccacgcc gaggaccacc catcgaacga agtcaccggt
       61 gtcgcgcagg agacgcgcga tgcccaacgc gaccgcggcc gtaaccgccg ttacccccgc
      121 ccaccggacg atctctagaa gggcgatcgc gagacccgct ccgtgaagga cggatcgagc
      181 cgggtcgatg aagatcgtcg cgagggcgag agccacgagg gccacctcga accctcccca
      241 gacgtgatcc gttcggtcgc cgggggcagg ctccaagaac cacacggcca ggacgacgac
      301 cgcggccccc agcgcgatcc cgcacgcgat cgcggcggcg ggtggtccga ccgtcacggt
      361 ggggccgaga accgcttgca ccccccgcaa ttcggccaga tcgagggtgc cccagcgcag
      421 cgcaccgatg cccacggctc cggcgatcac ggcgagggag agaacggccc gggccgaggg
      481 aagcagcggc aaaagcagcg ccctcccgaa ggtgatcagc gcgagacccc cgacgacggt
      541 gacgaggggg ccgggcacca gccccagtag ataggccatg gcgatgacgg ctccggcgac
      601 gcgtgcggcg gccgagaagg gcgctagggg ggccgcgacc ccatccgttt ggatcaagcg
      661 cggcggcggt tgcgaccgtt agacgagcgg gacccggttc ccggagtagt gccgttggtg
      721 ccgtagagac gccccgcata gaacgcgcgc acgagatggt tccacgcaca gtcgatgcac
      781 acctcgacga tgtagcacgt gaactggtcg tacttggtcg tcagctcctc gagccattcc
      841 agcggataga cgaccttgcc gttgttatgg cgcagctcgt cgccgtatac gtagcggacc
      901 caccgcaaag cgtcgtggga gcagacgggg cagcgatcct gcaccggctc accgatgttc
      961 ttgcccgccc gaaggagctc ggggtgggcg tcgcagagat cggccgtatc cagcgacccg
     1021 cgcgtgaggg accccagagc ggcacgcctg gctaggcggt aatcgaccga aggaccggcc
     1081 ggtgggggcg gcatcctggt ggggtcgaag gaccgtccgg tgacatccat gaaggcgatg
     1141 gtaaacccgt ctacgccgta ctcctagggg ttcccaagct ctgccaggtt ggcatttgac
     1201 cgaaccgggg tatcatgttg atatatcgag ccgatatatc cgaacgacct atccgcacgc
     1261 gatatccagg gacaggaagg tacatgagac aacccggcat gctcgaccta gccgtcttgg
     1321 gactcctcaa ggagcgcgcc atgcacggtt acgagttgcg caaacagctc ggcgccatgc
     1381 tgggcccctt ctggcaggtc tcgtggggct ctctctaccc gacgttgcga cgtctagcca
     1441 agttcggtgc cgtcgagaag ctcgacgcgc cgctccgcaa gaccactcgc gcagcgaaga
     1501 caaccggcaa gcgttccgcc cgcgccacca aatccaagtc caagtcgacg accttgtcgt
     1561 cggggcgcag gaagaacgtc taccagatca ccgctaaggg cgaaggcatg ttcgtcgcga
     1621 tgctcgaaga gaccgcagcg gcgacggatg ccgagcactt caccctcaag ctcgcgttct
     1681 tccgttacct gcaacccgag atgcgtcttg cgctgctcga gcgccggcgc gcgtacctgc
     1741 aagaaaagct tgctcaattc aagaccaaca tccgcgacta caaggagcgg gtggacagct
     1801 acacgctctc cctccagaac catgggatgg cggcaacgca gagcgacatc gattggatcg
     1861 acgaactgat cacagcggaa cgggcgctcg atcgtgttcg tcccacgaaa gcgtaggggg
     1921 aacgatggca aaggtaagag tagggatcgt cggcgctggg aattgcgcgt cgtcactcgt
     1981 ccaaggcgtc gagtactacg cgaaagcacg agcagacgag cgcgtgccgg gcctcatgca
     2041 cgtggacgtc ggtgcctatc acgtgtcgga catcgaattc tcttgtgcgt tcgacgtgga
     2101 cgccaacaag gtgggcctcg atctcgccga cgcgatctgg gtcgagccga acaacacgat
     2161 caagttctcg gacgtcccct cgctggacct caaggtcgag cgcggacaca cctacgacgg
     2221 tctcggcagc ttctacaagg acgttgtcac cgagtccgag ctccctccgg tggatgtggc
     2281 caagatcctg cgggatacgc acacggacgt cctcgtcaac tacctcccgg tcggatccga
     2341 gcaggcggct cgcttct
//
LOCUS       DATMED010000031         3832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3095502, whole genome shotgun sequence.
ACCESSION   DATMED010000031 DATMED010000000
VERSION     DATMED010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3095502"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1021
                     /locus_tag="VNP73_00780"
     CDS             107..1021
                     /locus_tag="VNP73_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL64486.1"
                     /translation="MTDFSLIDTKMHGMPEITAAFLVVGRQVALVETGPKSVVGNVFD
                     ELDRLGIDRLDWILVTHIHLDHAGAAGTLAERFPDARVGVHEVGAPHLADPSKLWKSA
                     SRIYGERMEKMWGGIDPIPQERIEVLDDGREIDLGGRSLKAIETPGHAYHHHAFLDSQ
                     SGTLFTGDALGVRLPGVPHIRPATPPPEFHLEKAVASIERIRKLAPAALAPTHFGPID
                     GDVESSCDDAVAALETWANWVQEVRKQTDDLDEAAAMVERKAREDLTGRLDDAAIDRL
                     EQTTSFWMNTWGYMRYFDKQEEGARGNQ"
     gene            complement(1073..1498)
                     /locus_tag="VNP73_00785"
     CDS             complement(1073..1498)
                     /locus_tag="VNP73_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL64487.1"
                     /translation="MTIVELTTDDEFQEAYAVMKELRTHLTTALYTELLAEMRPRGYR
                     MFAVRDGGQIVALAGVTFATNLYYQRYLWVFDLITTEAGRSHGHGKALIEHLEELARN
                     AGCDTIALSSSFPRVDAHRFYEEKVSFERSGYTFKKELD"
     gene            complement(1495..2199)
                     /locus_tag="VNP73_00790"
     CDS             complement(1495..2199)
                     /locus_tag="VNP73_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWL64488.1"
                     /translation="MHKGRDGRPRKPNGPPPESQYESVHLAKEVVAELRATARPGKGD
                     ILVKVFAEAVAAFAAEDFEEAVRLGEQAKHIALRAIGPREFLGLAYYKLGRFRDAARE
                     LAAFRRLSGSTDQNPVLADSYRALEKPDKALTYVDEVTRAAPPEVYYESQIVAAGALS
                     DMGRTAEAIARLETLDLDPVVAEPHHVRAWYALGDLLERSGRFTQARDLFEAADSADP
                     GATDAGSRADRLRASR"
     gene            2560..3054
                     /locus_tag="VNP73_00795"
     CDS             2560..3054
                     /locus_tag="VNP73_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64489.1"
                     /translation="MLTATDPQDGVWKIKRHWISRKPRLRWRKGSEEGLEALEFVGGI
                     FDDNPFGIIVGLIAVVLLLLLILPVFAFLIELLIVGVLLAVTLAAKILFRRPWIIEAW
                     QEGARDQHLRWAVVGWNRSGEVMDEVARTIASGGTFLSPHATPINPGPSTQTERAAPE
                     GGSR"
     gene            complement(3058..3174)
                     /gene="rrf"
                     /locus_tag="VNP73_00800"
     rRNA            complement(3058..3174)
                     /gene="rrf"
                     /locus_tag="VNP73_00800"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3286..>3832)
                     /locus_tag="VNP73_00805"
     rRNA            complement(3286..>3832)
                     /locus_tag="VNP73_00805"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          714 a         1177 c         1198 g          743 t
ORIGIN      
        1 tttcgatcac ggcgcgtcgg tcgatgtcgg tgagccgtcc tgcgtagggg ttcacgatga
       61 ggatcgcagg aggacgctct ccgggggcta tcatcccgcc aacgatatga cggacttcag
      121 cctcatcgac acgaagatgc acggcatgcc ggagataacg gcagcctttc tggttgtcgg
      181 gcggcaggtc gctctggtgg aaacagggcc gaagtcggtc gtcggcaacg tcttcgacga
      241 gctcgatcgg ctcgggatcg accgcctcga ttggatcctc gtgacgcaca tccatctgga
      301 tcacgcgggg gcggccggca cgctcgcaga gcgcttcccc gatgcgcgcg ttggggttca
      361 cgaggtcggg gcaccccatc tcgccgaccc gtcgaagctg tggaagagcg cgtcgaggat
      421 ctacggagag cggatggaaa agatgtgggg ggggatcgac ccgatccccc aagagcgcat
      481 cgaagtgctc gatgacggac gcgagatcga cctcgggggc cgctcgttga aagcgatcga
      541 gacaccggga cacgcctatc accatcacgc gttcttggac tctcaatcgg gaaccctgtt
      601 caccggagat gcgttggggg tccgtctgcc gggagtacct cacatcaggc cggccacccc
      661 tccgccggag ttccatctgg agaaggctgt tgcttcgatc gaaaggatcc gcaagctggc
      721 tcccgcggcc ctcgccccca ctcacttcgg accgatcgac ggcgatgtgg agtcgagctg
      781 tgacgacgcg gtcgcggccc tcgagacgtg ggcgaactgg gttcaggagg ttcgcaagca
      841 gacggacgac ctcgacgagg ctgcagcgat ggtcgagcgc aaggcccggg aggacctgac
      901 cgggcgtctc gacgatgccg ccatcgaccg gttggagcag acgacgtcgt tctggatgaa
      961 cacgtggggc tatatgcgct acttcgacaa gcaagaggag ggggcacggg ggaaccagtg
     1021 agccggcttg ccggcggggt tcccccggat cggagatcaa ttgggaagcc tcttagtcca
     1081 gctccttctt gaaggtgtag ccgctccgct cgaacgacac cttctcttcg tagaaccggt
     1141 gagcgtcgac gcgcgggaag ctcgacgaca acgcgatcgt gtcgcaccct gcattgcgag
     1201 cgagctcctc gaggtgctcg atgagagcct ttccatggcc atgcgagcgg ccggcctcgg
     1261 tcgtgatcag atcgaacacc catagatatc gctggtagta gaggttcgtt gcgaacgtga
     1321 caccggccag agcgacgatc tgtcccccat cgcgcactgc gaacatccgg tagccgcgcg
     1381 gacgcatctc cgcgaggagc tcggtgtaga gcgccgtggt cagatgggtc ctcaactcct
     1441 tcatcaccgc atacgcctct tggaactcgt cgtctgtcgt gagctcgacg atcgtcatct
     1501 cgacgctcgc agtctgtcgg cgcgcgaacc ggcatccgtg gcgcccgggt ccgccgagtc
     1561 ggccgcttcg aataggtcac gggcttgcgt gaaccgcccg cttcgctcga gcaggtcgcc
     1621 cagcgcgtac caggccctta cgtgatgggg ttccgctacc accgggtcga gatccagcgt
     1681 ctcgagcctg gcgatcgctt ctgccgttcg gcccatgtcg gagagagctc cggcagcaac
     1741 gatctgcgac tcgtagtaga cctccggcgg cgccgcccgg gtgacctcgt ccacgtatgt
     1801 gagagccttg tcgggtttct ccaaagcgcg gtaggagtct gcgagcaccg ggttctgatc
     1861 cgtcgagccg gacagtcgcc tgaaggcggc gagctcccga gccgcgtcgc ggaaccgacc
     1921 cagtttgtag taggcgaggc cgaggaactc gcgggggccg atggctcgca gcgcgatgtg
     1981 cttggcttgt tcaccgagcc tgaccgcttc ctcgaaatcc tctgcggcga aggcagcgac
     2041 cgcctcggcg aagaccttga cgaggatgtc tcccttgccc ggtcgtgcgg tcgctctcaa
     2101 ctcggcgacg acttctttcg cgaggtgtac agattcgtac tggctctccg gaggggggcc
     2161 gttgggtttg cgaggccttc catctctgcc cttatgcatg tcggaaccct aagtcgccct
     2221 cacgcgaagc gaccccgccg ttaagcgggg ccgcttcgtg cgaaagacgt gcctcgtgct
     2281 aactcttcca gccgagacaa ccgaacccgt caacctgaat gcaatcggct tgagctggtg
     2341 ccgagaacaa gccgaagatc gacaggcccg tggtcaagaa aatgaccgcg agtctcttcc
     2401 gcatccgccc cccttccatt tccgccatac cgcccgcgtg gaacccgctg ggagattatg
     2461 gctaaacggg cgtttcgggc aaatccaccc gaataggggc aagagttccc ccgtaagtag
     2521 ggcgagcccg cctgcgttgg ctgatggttg gatgaggggg tgcttacggc gacggacccc
     2581 caggacgggg tctggaagat caagcggcac tggatctcac ggaagcccag gctgcgctgg
     2641 cggaaggggt ccgaagaggg gctcgaggcc cttgaattcg tcggcggcat cttcgatgac
     2701 aaccccttcg gaatcatcgt agggctcatc gcagttgttc tcttgttgtt gctgatcctt
     2761 ccagtgttcg cgttcctgat cgagctgctc atcgtggggg tccttctggc cgtcactctt
     2821 gcagccaaga tcctttttcg cagaccttgg atcatcgagg cgtggcaaga gggggcaagg
     2881 gatcagcacc tccgatgggc cgttgttgga tggaatcgca gcggcgaggt tatggacgag
     2941 gtcgcgcgaa cgatcgcgag cgggggaacg ttcctgtcac cccacgcgac gccgatcaac
     3001 ccgggtccgt cgacgcaaac agaaagagcc gccccggagg gcggctctcg ataaaaaacc
     3061 cggcggcgtc ctactctccc accccgtctc cagggcagta ccatcggcgc tgaagggctt
     3121 aacttctggg ttcggaatgg gaccaggtgg ggccccttcg ctatagccac cggaaactta
     3181 tttagttgtc aagcgatcgt ctcgacgatc gcgtcacctc catgcggtcg ccggctccat
     3241 tcggagcgac aacctgaacc ctggaatctc cagagcgagc gcgagcaaac caagtcctcg
     3301 gcctattagt accggtcgcc tgaacacgtt gtccgtgctt acagctccgg cctatatacc
     3361 ttgtcgtcta gcaaggggcc ttacccggtt aacccggtga gagatctcat cttcaggtgg
     3421 gcttcgcact tagatgcttt cagcgcttat cccatccgca cgtagctaaa cagcatgccg
     3481 ttggcacgac aactgtcaca ccagaggtgc gtccgtcccg gtcctctcgt actagggaca
     3541 gctcctgtca aatctcttac gcccacggag gatagggacc gaactgtctc acgcatgtat
     3601 ctcccacatt actgcgggca tagactatac cttcacccca tttctggggg ccggcgtgtt
     3661 atgagtctca tagatttgcc aaaaggcagg agacccatct ccccgatcga gcgagatcga
     3721 ggaagtcgtt acggggacca gccaaccctt gctacacgga tgttgctcga catccgcgac
     3781 tgatagaccg cttcgtatct tcgtatctag gaatagttta gggttttgat gg
//
LOCUS       DATMED010000032         4356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5393485, whole genome shotgun sequence.
ACCESSION   DATMED010000032 DATMED010000000
VERSION     DATMED010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5393485"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..719)
                     /locus_tag="VNP73_00810"
     CDS             complement(138..719)
                     /locus_tag="VNP73_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWL64490.1"
                     /translation="MGKVTSEISMSLDGFITGPNVSVGNGMGDDGDRLHDWRFDAKTE
                     TDAAIVDEIYSSTGAVLLGKRMFDVGFEPWGDPPPFRMPVFVVTHEAREPLPKQGGTT
                     YTFVTEGIEAALEQARAAADDKDVGVWGGANIMRQYLKAGLLDELQIHLVPILLGGGV
                     RLFEDLDPEGIELRRTSSIETPGATHFRFEVVK"
     gene            complement(910..1041)
                     /locus_tag="VNP73_00815"
     CDS             complement(910..1041)
                     /locus_tag="VNP73_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64491.1"
                     /translation="MDASGRPPHDAKDCALIREGGEVLERAGQVLVADAPAPGVGWP"
     gene            complement(1235..3964)
                     /locus_tag="VNP73_00820"
     CDS             complement(1235..3964)
                     /locus_tag="VNP73_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HWL64492.1"
                     /translation="MPILIAVALALPMLGMSSAPAQENDSADAIDYVEGNAAKLGITR
                     ADVANVFVTSKYTSSHSGITHVNLNQRYKGLEVFGGHATVNIAADGEVVFAAGAFVRD
                     LSAGASGDDDLDATEAVEAAADELELDEPENLRVLSQESGLAEESVLSRAGISNEPIE
                     ARLGWQPTKQGLRLAWQVTIDDSSDVHLWNAAVDAESGELLKVDDWTHEDSYESLAST
                     LMRTNSTSASAAASEPPLPPSPAIDGSSYRVFQIPREGPNDGPRALVENPADDRFSPF
                     GWHDTDGVAGAEFTNTRGNNVHAYLDQDRNNQPDFGDTEGGPGLDFDFPIDLNEHAQA
                     YREAVVTNLFYGCNIVHDITSLYGFNEAADNFQTINYGGGPFSNGDHVRCEAADGNGT
                     NNANFSTPSIGGTPRMQMFLWPGNQLGSQNQVAVDGGASFGAGWARFGPPAPNAGLTG
                     QFFDAGNGCLPADYAGAPAGDWIAIVVGGTTGCEPIEKTRQADNAGASAIVMTTGTGT
                     TPSTAIIAPNLGGMAVASPGIPAVAITEADGNALRGALPASGAVRKHPNHPGIRDGDF
                     DFAIIGHEYGHGVSNRLTGGPGINCLSGNEQAGEGWSDYIGLTIPLDPALDDPDQPRG
                     LVPYVLFQPDRHGNGLRPRPYTRDMTIQPFTYDSIKTNGWLNGTSLALPHGLGHGWNA
                     VLWDMNWDLIDKHGFHANLYDGWEAGGNTRAFQYVMDGLKIQGCGPGLVVARAAIIAG
                     ADELSDGEDTCTLWATFARRGLGFSAVQGTTNRNDNTEAFDTHPDCRRGFQPPVSHTY
                     GSLRDVDAGDTVPLRFTADGYRELDVLASNSPFSRRVDCETLQVPSITPGIITPREYP
                     IDTETPGNSKLTVNSNGVFLYNWETLEDWGGTCREVVVTRDDGKQHRAFFRFE"
BASE COUNT          747 a         1445 c         1347 g          817 t
ORIGIN      
        1 acaggagacg aggtagccgg ccgtcctgtg acagccaagg gaaagagagc gcagatgatt
       61 tcggatcgca ccatcccgag gcgatcatct tcacgacgac cgcgccttag gaagtcaaga
      121 atcgaggatt acgtgtgtca cttcaccact tcgaaacgga agtgggtggc gcccggtgtc
      181 tcgatcgaac ttgtcctccg tagttcgatt ccctcagggt cgagatcctc gaagagccgg
      241 acacctccgc cgagcagtat ggggaccagg tggatctgca actcgtccag caacccggcc
      301 ttcaggtact gcctcattat gttcgctccg ccccagacac cgacgtcctt gtcgtcggcc
      361 gcggcacggg cctgctcgag tgctgcctcg atcccttctg tcacgaaggt gtaggtcgta
      421 ccgccctgct tgggcagggg ctctcgcgct tcgtgagtca ctacgaacac cggcatccga
      481 aacggcggag ggtcgcccca tggctcgaac ccgacgtcga acatccgctt ccccagcagg
      541 accgccccgg tcgaggaata gatctcatcc acgatcgcgg catcggtctc cgtcttcgcg
      601 tcgaacctcc agtcatgaag ccgatcccca tcgtccccca tcccattacc gacactcacg
      661 ttcggtccgg tgatgaaccc gtcaagcgac atcgagattt cggacgtgac cttgcccatg
      721 gcagccctcc ttatcgcatc ggtctttgaa agctagacgg ctcccggata gagaactcat
      781 ctccttcgtc cgaacacctt cttgttcacc tttaaccact tgtcgtccca agggtgaccc
      841 tcgaataccc ccggcaaact gagagcagac tcaagaatgc atcgtcggat ccccgcggtc
      901 ccatcgcgtt tacggccagc ccacccccgg cgccggcgcg tcggcgacga ggacctgtcc
      961 cgcgcgttcc agcacctcac cgccctctcg gatgagcgcg cagtccttgg cgtcgtgcgg
     1021 tggacgccca gatgcatcca tcggcatcga agcagatgcc atcggggcga ctcgccgata
     1081 gcgagtcctt actcatcgga accacacgcc tggaccccga ctcaattcaa aacctgggca
     1141 tcacggcacc actcgcgaac ggtatccccc gcccggcaga cgatgggagc gcccgtgtcg
     1201 gggcgctccc atcgattctt gcttcgcagc aggtctactc gaaccggaag aaggctctgt
     1261 gctgcttgcc gtcgtcgcgc gttacgacga cctcacggca ggtgcctccc caatcctcca
     1321 acgtctccca gttgtagagg aagactccgt tcgaattcac ggtgagcttc gagttgcccg
     1381 gggtctccgt atcgatcggg tactcccgcg gcgtgatgat gcccggagtg atgctgggca
     1441 cctgcagcgt ctcgcagtcg accctgcggg agaagggtga gttgcttgcc aagacgtcga
     1501 gctccctgta accgtccgcg gtgaacctga gcggcacggt atcgcctgca tcaacgtccc
     1561 taagggagcc gtacgtatgg gagaccggag gctggaagcc tcgccggcag tccggatgcg
     1621 tgtcgaacgc ctcggtgttg tcgttgcgat tcgtcgtccc ctgcactgca ctgaaaccga
     1681 ggccgcggcg ggcgaacgtc gcccacagtg tgcaggtgtc ctccccgtcg ctcagctcat
     1741 cggcgccggc gatgatcgcc gcccgtgcga cgaccagccc tggaccgcac ccctgaatct
     1801 tgagcccgtc catcacgtac tggaaggcgc gggtgtttcc gcccgcttcc cagccgtcgt
     1861 acaagtttgc gtggaacccg tgcttgtcga tcaggtccca gttcatgtcc cacaggaccg
     1921 cgttccagcc gtgcccaaga ccgtgcggga gggccagcga ggtcccgttc agccatccgt
     1981 tcgtcttgat cgagtcgtac gtgaacggtt ggatcgtcat gtcgcgcgtg tacggccgcg
     2041 ggcgcagccc gttgccgtgc cggtccggct ggaacagcac gtacggcacg aggccgcgcg
     2101 gctggtcggg atcgtccaga gccgggtcga gcgggatcgt tagaccgatg tagtcgctcc
     2161 aaccctcacc cgcctgctcg tttccgctca gacagttgat ccccggtccg cccgtcaggc
     2221 ggttggagac cccgtgtccg tactcgtggc cgatgatcgc gaagtcgaag tcgccgtcac
     2281 ggatccccgg gtgattcggg tgcttgcgca ccgcgcccga cgcgggtagc gccccacgga
     2341 gcgcgttgcc gtcagcctca gttatggcga cggccggaat cccgggcgac gcgacagcca
     2401 tcccacccag gttgggggcg atgatcgccg tgctcggcgt ggttccggtg cccgtcgtca
     2461 tgacgatggc actggcgccg gcattgtccg cctgacgggt cttctcgatc ggctcgcatc
     2521 cggtggtgcc gccgacgacg atcgcaatcc agtcgccggc cggtgccccc gcatagtccg
     2581 cgggcaggca gccgttgccg gcatcgaaga actggcccgt aagaccggcg ttcggagccg
     2641 gcgggccgaa ccgcgcccag ccggcgccga agctggcacc gccgtcgacg gcgacctggt
     2701 tctgggaccc gagctggttg cccggccaca agaacatctg catcctcggt gtcccgccga
     2761 tcgacggagt gctgaagttt gcgttgttcg tcccattccc gtcggcggcc tcgcagcgga
     2821 cgtggtcgcc gttcgagaac ggaccgccgc cgtagttgat cgtctggaag ttgtcggcgg
     2881 cctcgttgaa gccgtatagc gaggtgatgt cgtgaacgat gttgcagccg tagaagaggt
     2941 tcgtcaccac ggcctcgcgg taggcctgcg cgtgttcgtt caggtcgatc gggaagtcga
     3001 agtccaggcc cggcccgcct tcggtgtcgc cgaagtcggg ctggttgttg cggtcctggt
     3061 cgagataggc gtggacgttg tttccccgcg tgttcgtgaa ctccgcgccg gcaacgccgt
     3121 cggtgtcgtg ccaaccgaac ggagagaagc ggtcgtcggc gggattctcg acgagcgcgc
     3181 gcggcccgtc gttcgggccc tctctcggaa tctgaaagac acggtagctc gacccgtcga
     3241 tggcgggact cggcggaagg ggtggctcgc tcgccgccgc cgacgcgctc gtggagttcg
     3301 tgcgcatgag cgtgctcgcg agactctcgt acgagtcctc gtgcgtccag tcgtccacct
     3361 tcaggagctc tcccgactcg gcgtcaaccg ccgcgttcca taggtggacg tcggaagagt
     3421 cgtcgatcgt gacctgccag gcgaggcgca gcccctgctt cgtgggctgc caaccgagac
     3481 gggcctcgat cggctcgttg gagatgcccg cacgcgagag gacactctcc tcggcaaggc
     3541 cgctctcctg actgagcacg cgcaggttct cgggttcgtc gagctcgagc tcatcggccg
     3601 ctgcttcgac agcctcggtg gcatccagat catcgtcgcc ggaggccccg gcgctcagat
     3661 cacgcacgaa cgcgcccgct gcgaagacaa cctctccgtc ggccgcgatg ttcaccgtcg
     3721 cgtgtccccc gaagacctcg aggcctttgt agcgctggtt caggttgacg tgggtgatgc
     3781 cgctgtgcga actcgtgtac ttgctggtga caaagacatt ggcgacatcc gcgcgcgtga
     3841 taccgagctt cgccgcattg ccttcgacgt agtcgatagc gtctgcggaa tcgttttcct
     3901 gcgccggtgc agagctcatc ccaagcatcg gtaaagcgag cgccaccgcg atcaagatcg
     3961 gcacaatttt tcgcacccca aatgaggact tcaccgcgta tccccccttg taaaaggagc
     4021 cgccctaggc tcccgtcgaa gggctctccg cgccccccga gcaaagtaag caaattatgc
     4081 aatcaggttt cctttatcgt caatggtggg ttttactacc agcaagtcgc ccgagttcgc
     4141 cttccaagaa aagaccacgc gatgcgtact cggcccgaag ggaatcaact tctgcagaga
     4201 agcgaaaacc gccgaggtcg catcgcggtg cggtgcaagg acgtcaggag gcggcgggtt
     4261 ctgtcctcgg ggcacgcgtc atgaaaaccg ccgccgactc ggcagtgagc accaagagcg
     4321 ttatggctgg atggaggaaa gccgctcctg aagcca
//
LOCUS       DATMED010000033         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1563726, whole genome shotgun sequence.
ACCESSION   DATMED010000033 DATMED010000000
VERSION     DATMED010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563726"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VNP73_00825"
     CDS             <1..1044
                     /locus_tag="VNP73_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWL64493.1"
                     /translation="ELDALLEHYAGHLPGPDADLVREIVETALKMGEEQVSRLDRKIV
                     NTALKELRHAFRVFAPYKGVRKVSIFGSARTLVGDPAYVAARDFSALIVKEGWMVITG
                     AGPGIMEAGHEGAGGEKSFGVNIVLPFEAKPNPVIADDQKLINFKYFFTRKLTFMKEA
                     DAFVLLPGGFGTMDEAFELLTLMQTGKSDLHPVVLLDCPGGDYWKDFDRFVREDLVSR
                     GYVGEDDLNLYRIVDDPQAAVDEIDDFHRVFHSQRVVGRKLILRLNVMPEESILATLS
                     DEFADILSRPIREAKPSTAELRDEDVPDLPRIALAFDRQHVGRLRRLIDRLNELGPTP
                     TSAPAVKGDPRNL"
     gene            complement(1152..1337)
                     /locus_tag="VNP73_00830"
     CDS             complement(1152..1337)
                     /locus_tag="VNP73_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64494.1"
                     /translation="MEFDKMPAAQVVLAFARLVDEALASSGIENARRAVADADSKRRA
                     RTALEQLDGRDSFDRTA"
     gene            complement(1418..2389)
                     /locus_tag="VNP73_00835"
     CDS             complement(1418..2389)
                     /locus_tag="VNP73_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWL64495.1"
                     /translation="MSDGFKVPRSASAIGFVLVALAAATWGSDGLLRGKLALELPAAT
                     VVMVEHVILVLLTAPLLIRVIPRLRGLTAKDWISLVLIGAGASALATVLFTQAFVYGD
                     PNTPLLLQKLQPLFAIGGATLLLKEKILPRFVIYLVFALGGAYLITFPEPTNVSVSAL
                     APALLALAAAGLWGFGTVLGRHMTTKIQFAELTALRFAIGLPATIAIVLVQDGGKALG
                     SIGGREIGALLLLALLPGLFALLLYYRGLRETPASAATLAELAFPISAITINYFAFDA
                     VLVSTQWLGVAVLSGTIVAMGLAASRGTRAIGIELPAGLREEQPAHA"
     gene            complement(2399..2809)
                     /locus_tag="VNP73_00840"
     CDS             complement(2399..2809)
                     /locus_tag="VNP73_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWL64496.1"
                     /translation="MDVDEAKEFIRSNHRAILITHRKDGRLQTSPVAVGIDAEGMAVI
                     SSRETAYKTKNLRRDPHATATVFTTDWYGPWVQIEGEATIVSLPEAMDGLVDYYRGIA
                     GEHPDWDDYRAAMEKEKRLLIKIDIAQAGPSSSG"
     gene            2900..3166
                     /locus_tag="VNP73_00845"
     CDS             2900..3166
                     /locus_tag="VNP73_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HWL64497.1"
                     /translation="MICEHFDQARVFATDVRECKECVDMGSRWVHLRLCLTCGHVGCC
                     DSSPNKHASNHFHATGDPVMRSLEPGEVWGFCFVDDLFVEELPV"
     gene            3163..>4115
                     /locus_tag="VNP73_00850"
     CDS             3163..>4115
                     /locus_tag="VNP73_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL64498.1"
                     /translation="MNNPETLEQIAYGTPRGRWVVTAAVLGTALAFLDATVVNVALPN
                     IGQDLDATLGDLQWTVTGYMLTLSAWILTGGALGDRYGRRRIFIVGTVWFAAASLLCG
                     IAPNVPTLVAARAFQGIGGALLTPASLAIIEASFRKEDRGRAIGAWSGLGGVAAAVGP
                     FVGGYLLSIATWRVIFLINIPMAAAIVWVTLRHVPESMDPRAPRQPDIAGAALGALGL
                     GGVVYALIRASEGGFGQPVVWVTLAVGVIALISFGVAEVRGKHPMLPLDIFSSRQFTG
                     ANLVTFVVYAALGGIFFLLVMNLQQAVGYSPLEAGVATLPVT"
BASE COUNT          722 a         1274 c         1299 g          820 t
ORIGIN      
        1 gagctcgacg ctcttctaga gcactacgcc gggcatctcc ccggacccga cgccgatctg
       61 gttcgggaga tcgtcgagac cgcgctcaag atgggcgagg agcaggtcag ccggctcgac
      121 cgcaagatcg tcaacacggc cctcaaggag cttcgccacg ccttccgcgt cttcgctcct
      181 tacaagggag tcaggaaggt ctcgatattc ggctcggccc gcactctcgt cggtgatccc
      241 gcctacgtgg cggcccgcga cttctccgcc ctgatcgtca aggagggttg gatggtcatc
      301 acgggagccg gccccggcat catggaggcg ggtcatgagg gggccggggg cgagaagtct
      361 ttcggggtga acatcgtcct gccgttcgag gcgaagccga atcctgtgat cgccgacgat
      421 cagaagctca tcaacttcaa gtacttcttc actcggaagc tgacgttcat gaaggaggcc
      481 gacgctttcg tcttgctccc gggcggcttc gggacgatgg atgaggcctt cgagctcctc
      541 acgctcatgc agaccggcaa aagcgatctg cacccggtcg tattgctcga ctgtccggga
      601 ggcgactact ggaaggactt cgacaggttc gtgcgggaag acctggtctc tcgtggctac
      661 gtcggcgagg acgacctcaa cctgtaccgg atcgtcgacg atccccaggc ggctgtggac
      721 gagatcgacg acttccaccg cgtcttccat tcgcaacgcg tcgtcggccg gaaattgatc
      781 cttcgactga acgtgatgcc ggaagagagc atcctggcta cgttgtcgga tgagttcgcg
      841 gacatactca gcaggccgat ccgcgaggcc aaaccttcta ccgccgaact acgtgacgag
      901 gacgttcccg atctacctcg catcgcactt gccttcgatc ggcaacacgt cggacggttg
      961 cgccgcctca tcgaccggtt gaacgagttg ggcccgacgc cgaccagcgc tccggccgtc
     1021 aagggcgatc cgcgaaacct ctaacagaag caacgagggg ctccgggtgg aagcccctcg
     1081 tcagattgga ccctgggggg agtccttctt gcccggtagc cgggggagtg acccaccgga
     1141 caagcttcgc ctcaagcagt tcggtcgaag gagtcccttc cgtcgagttg ctcgagggca
     1201 gtccttgctc tgcgtttcga gtctgcgtcg gcgacggctc gacgggcgtt ctctatcccg
     1261 gagcttgcga gagcttcatc gactaggcgt gcgaaggcga gcacgacttg tgcagcgggc
     1321 atcttgtcga attccatgtc cgagaagata agggcggatt gttacgggag cgttccctct
     1381 gcgttaattg cgtaggaagt ttcttaggaa acttctgtca ggcgtgagcc ggctgttcct
     1441 ctcgcaaccc cgccggtagc tcgatcccga tcgctctggt ccctcggctt gcagccaacc
     1501 ccatcgccac gatcgttccc gacagcacgg cgacgcccaa ccattgcgtg gaaacgagca
     1561 cggcgtcgaa ggcgaagtag ttgatcgtga tcgcactgat cgggaacgcc agctcggcca
     1621 gcgtggccgc cgacgcaggg gtttcccgca gccccctgta ataaaggaga agtgcgaaca
     1681 gccccgggag aagcgccagc agcagcaggg caccgatctc gcggcccccg atcgacccga
     1741 gtgctttgcc cccgtcctgc accagaacga tggcgatcgt tgcgggcaga ccgatggcga
     1801 aacgaagggc ggttaattcg gcgaactgga tcttggtcgt catgtgccgt cctagaaccg
     1861 tgccgaatcc ccataggccg gcagcagcca gcgccagcaa cgccggcgcc agtgcggaca
     1921 ccgagacgtt ggtcggttcg ggaaaggtga tcaggtaggc accgccgagg gcgaagacga
     1981 gatagatgac gaagcgggga aggatctttt cctttaggag caacgtcgcc ccgccgatcg
     2041 cgaacagcgg ctgcagcttc tgaagcaaca gtggcgtgtt cgggtcgccg tagacgaagg
     2101 cttgcgtgaa gagcaccgtc gcaagagccg aagcccccgc cccgatgagc accagcgaga
     2161 tccaatcctt cgcggtcaag ccacgcaggc gtgggatcac tcggatcaat aggggagccg
     2221 tgaggagaac gaggatcacg tgctcgacca tcacgacggt tgcggcggga agttcgagtg
     2281 ccagctttcc gcgcaataag ccgtcgctac cccacgttgc cgccgccagc gcgacgagga
     2341 cgaatccgat cgccgaagct gatcgaggca ccttgaaccc gtcgctcatg cagggaatct
     2401 agccgctgct gctgggccct gcttgtgcga tatctatctt gatgaggagc cgcttctctt
     2461 tctccatcgc agctcggtag tcgtcccagt cggggtgctc cccagctatc cccctgtagt
     2521 aatcgaccaa cccatccatc gcctcaggga gagagacgat cgtcgcctcg ccctcgatct
     2581 gtacccatgg gccgtaccaa tccgtggtga atacggtcgc ggtcgcgtgc ggatcccgcc
     2641 tcaggttctt ggttttgtag gccgtctcac ggctgctgat aaccgccatg ccctcggcgt
     2701 ctatccccac cgctacggga gaggtctgaa gtcttccgtc tttgcggtgg gttatcagga
     2761 ttgcgcgatg gttgctcctg atgaattcct tggcttcgtc cacgtccatg gtgacctcct
     2821 tcgtcggttc gttcagcgtt tccccgacgg cccttattcc accgggaaat atctcgcggg
     2881 gcttggtgtt gagagaaccg tgatctgcga acactttgac caagctcgcg tgtttgcaac
     2941 tgatgtccgc gagtgcaaag aatgcgtcga catgggtagc cgttgggtgc acctacgtct
     3001 gtgtttgacc tgcggacatg tcggctgttg cgactcctcg cccaacaagc atgcgtcgaa
     3061 tcatttccat gccaccgggg atcccgtcat gcgttcgctt gagccgggcg aggtctgggg
     3121 gttctgcttc gtcgacgacc tgttcgtgga ggagctaccc gtttgaataa cccagagacc
     3181 ctcgagcaga tcgcgtacgg caccccgcgc gggcgatggg tggtcacggc cgccgttcta
     3241 ggaacggccc ttgcgttcct cgatgcaaca gtcgtcaacg tcgccttacc gaacatcggc
     3301 caagatctag acgcgaccct cggcgatctg caatggaccg tgacgggcta catgctgaca
     3361 ctgtccgcgt ggatcctcac cggcggggcg ctcggcgatc ggtatggacg ccgccgcatc
     3421 ttcatcgtcg ggaccgtttg gttcgcggcc gcgtctcttc tttgcgggat cgctcccaac
     3481 gttccgaccc tagttgccgc gcgagcgttc caggggatcg gtggagctct cttgactccc
     3541 gccagccttg cgatcatcga ggcgtcgttt cgaaaagagg atcggggccg ggcgatcggc
     3601 gcgtggtccg gtctgggcgg tgtcgccgcc gcggtgggcc cgttcgtcgg cggctatctc
     3661 ctgagcatcg caacgtggcg agtcatcttc ctgatcaaca tcccgatggc cgcggcgatc
     3721 gtgtgggtca cgctgcggca cgtgccggaa tctatggatc cgagagcgcc gaggcagccg
     3781 gacatcgcgg gggccgcgct tggggcgctc ggtttgggcg gagtcgtcta cgcgctgatc
     3841 cgcgcttccg agggtggttt cggccaaccg gtcgtgtggg tcaccttggc cgtaggcgtt
     3901 atagcgctca tctctttcgg ggtcgcagag gtacgcggca aacacccgat gttgccgctc
     3961 gacatcttct cgtcgcgcca gttcacgggt gccaatctgg tgaccttcgt cgtgtacgcg
     4021 gcgctgggag gcatcttctt cctgctggtg atgaacctgc aacaggccgt gggttactcg
     4081 ccgttggagg cgggcgtcgc gacgctgccg gtcac
//
LOCUS       DATMED010000034         7633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3477073, whole genome shotgun sequence.
ACCESSION   DATMED010000034 DATMED010000000
VERSION     DATMED010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477073"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VNP73_00855"
     CDS             complement(<1..995)
                     /locus_tag="VNP73_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HWL64499.1"
                     /translation="MTSSVRLIKVDDIARMYDFGASHPLRPERVLLTYEHIRDLGLID
                     LDHVDESIARSATDEEILAVHDADFVRTVQGIDAGAYEPREGLEFGLGTPDDPIFPNM
                     HTASAAVCGASLSAAEAILEGAAQYAFNPAGGLHHARRREASGFCIYNDPAVAIAKAL
                     VVNPEWRVMYVDVDVHHGDGVQWIFYDEPRVLTVSLHQSGRYLYPGTGFEDEVGTGDA
                     TGTSVNVPLLPLTGEADYLWALENVLGPIAEAFRPDLLVTQLGGDTHWGDPLANLGLT
                     MTAYPAMARILHSVAREHASDRWLATGGGGYQAETIVPKIWTIHFAEMCGKPDAIP"
     gene            complement(1003..2652)
                     /gene="pruA"
                     /locus_tag="VNP73_00860"
     CDS             complement(1003..2652)
                     /gene="pruA"
                     /locus_tag="VNP73_00860"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HWL64500.1"
                     /translation="MSSFSILQPPAAHNEPVRDYGPGSPERRSLQTRLRAMQEERIDI
                     PMVIGGRDERSGDVYEAVMPHNTKHVLGEVHQSSAAHVESAIAASVEAHGDWAALPWQ
                     DRAAIFLRAAELLAGPWRDTVNAATMLGQSKTAHQAEIDAACELIDFWRFNVEYAGRI
                     YAEQPISAPGMWNQIDYRPLEGFVFAVSPFNFTSIAGNLPTAPALMGNTVVWKPSLTQ
                     AFSAHYLMELFEAAGLPDGVINMVHGPGPEIGEAVLASPELAGIHFTGSSPTFNWFWQ
                     KIGANIESYRHYPRIVGETGGKDFIVAHPSASVEALATAIIRGSFEYQGQKCSAASRV
                     YVPSNLWEALRARLADEVPNIAMGDVADFSNFMGAVIDDRAFAKHSNALDEARSEPGY
                     EVVAGGHAESSTGYFVEPTVVRTEDPRARLMRDELFGPIVTTFVYPEAEWAETLDLVD
                     STSPYGLTGAVFSTDEAAIVEARERLRFAAGNFYVNDKPTGAVVGQQPFGGARRSGTN
                     DKAGSMWNLARWASPRTIKETFSPPTDYRYPYMGADPNDRP"
     gene            complement(2672..3490)
                     /gene="proC"
                     /locus_tag="VNP73_00865"
     CDS             complement(2672..3490)
                     /gene="proC"
                     /locus_tag="VNP73_00865"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWL64501.1"
                     /translation="MAKLAVLGAGKMGEALVAGLLASGWRTPDEIAVTARRSERLEEL
                     ASKYGVQATTDNAQAIAGVEIVVIAVKPQDIETLLASIDTHVSPDQTVLSVVAAIPTA
                     FIESHLSSPIPVVRAMPNTPSVVHEGMAGISAGRHAGEDHLKLATDVLAHVGRVVIVE
                     EPYLDAVTAISGSGPAYFALLAEAMIEAGILLGLSREVSTQLVIQTMLGSAKLLRDTH
                     MHPVELREMVTSPGGTTTRAIQVLEQSGVRAAFLNAIQAAMERSQELARGEPEA"
     gene            3532..4446
                     /locus_tag="VNP73_00870"
     CDS             3532..4446
                     /locus_tag="VNP73_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWL64502.1"
                     /translation="MLGKAWLWASEKPRIQRFVAQGRIGRRVVERFVAGDTLEDAISV
                     IRALNEAGIGGIFDLLGEGVTDPAGAQAAAEEYLESIKRIEATGVDTTVTVKLTQLGL
                     AFDKGTCIDLLRVLAAEAHAIGTYVEIDMEQSDYATDTIDIFKLLRPDFPDLRLALQA
                     YLRRTLVDLEALAGGQPPKVRLVKGAYAEPTELAFQRDHEIDQQYRFITDWLFDRGVD
                     PAIATHDARLIQHARVSAETHGKSVKDFEVQMLYGVRRDLQERLASTGYRVRVYVPFG
                     SAWYPYLMRRIAERPANLRLFLRALAGK"
     gene            complement(4703..5950)
                     /locus_tag="VNP73_00875"
     CDS             complement(4703..5950)
                     /locus_tag="VNP73_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL64503.1"
                     /translation="MTDVRRLAVLTYHSSPLVEPGAGDAGGMTVYVRELATALAEQGV
                     LTDIFTRATGDLPRIASFGEGIRVIAIEAGPHRSLDKSQLEEHLSDFVSGIRSFSIGQ
                     RLSYDLVHSHYWHSGLAGRELAQVWGVPLVHSHHTLGRVKNRWLAPGDSPEPDERIAG
                     EAEVVEGADVLVASTEEERTHLQALYGAPEERLKVLPPGVDHNLFVPGDRAEARADLG
                     LGEGPLLLSVGRIQKLKGMDLSIRVVAELKARGVAARLLIVGGESGRLSHLEAGRLRA
                     LTRELGVDDLVVFAGRQPHIDLPLYYQAADALLVSSHSESFGLAALEAQACGVPVIGT
                     RVGGLPHLVAEGESGFIVDERDPSRLADRVQILFDDPDGWESFSRAARARAAVFSWKR
                     SAEAFLELYECLVREPYPEACTC"
     gene            complement(5952..6959)
                     /locus_tag="VNP73_00880"
     CDS             complement(5952..6959)
                     /locus_tag="VNP73_00880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL64504.1"
                     /translation="MALDVRVDCTERCFELPEWKELLERDPNRHIFATPEWNRLWWEQ
                     FGAGKDLFVLTMYSGEDLTAIVPLYRKPEDGRKIFRFNGGIDLTDYQGPVCSLDDRDV
                     VAETLVKWLAETDIEWDEFDAHNMPVPLGFAEFLVERADRDGFSWALDQEETTAVMTL
                     PDSWDEYLEHLDKKERHELRRKRRRLGRDHPDARIHSSDEDTLEEDLSKFIDLHRQAE
                     GTKGRFMGPEIAEFFKRIADCFASLGWLRLDTLSIGDRALASTFSFQYDNTFYLYNSA
                     FDPTIGRLSPGYILVSQLIEDAIEGGVRRFDFLRGPERYKYQFGARPLPLNNVRVLRH
                     P"
     gene            complement(7065..>7633)
                     /locus_tag="VNP73_00885"
     CDS             complement(7065..>7633)
                     /locus_tag="VNP73_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64505.1"
                     /translation="RDVVSAFTQGFLTRTPYPPPASALTTRSDGSDVPSFHVPTFQFL
                     MKEVTKMANTQAQDVLYAFVGLGDLAAEKVRSVSVPDVKSTTKLYNDSVKRGRGLWTK
                     IRNSAPTKQAVTQTKTARTQVKAAATSVTKAIQAGTSGSSTQLKQQTKTARTQVKAAG
                     TSVTKAVRANAKAARSAAGKVATTSKAS"
BASE COUNT         1436 a         2456 c         2382 g         1359 t
ORIGIN      
        1 gggatcgcgt cgggcttccc gcacatctcc gcgaaatgga tcgtccatat cttcggaacg
       61 atcgtctcgg cctggtagcc gccgccgccg gtcgcgagcc atctgtccga cgcgtgctcg
      121 cgcgccacgg aatggaggat ccgggccatg gccgggtacg cagtcatcgt cagacccagg
      181 ttcgcgagag ggtcacccca gtgggtatcg ccaccgagtt gcgtgacgag tagatccgga
      241 cggaacgctt cagcgatggg accgagcacg ttctcgagcg cccacagata atcggcttca
      301 ccggtgagcg gaagcagcgg aacattcacg gacgtgcctg ttgcatcgcc tgtcccgacc
      361 tcgtcctcga acccggttcc gggatacagg taacggcccg attggtgcaa cgacaccgtg
      421 aggacgcgcg gctcgtcgta gaagatccac tgcacgccat caccgtgatg aacgtccacg
      481 tccacgtaca tcactcgcca ttcgggattt accactagag ccttggcgat cgccactgca
      541 ggatcgttgt agatgcagaa ccccgaggcc tctctcctgc gggcatggtg aaggccgccg
      601 gccggattga acgcgtactg ggcggctccc tccaggatcg cttcggctgc agacaacgag
      661 gcaccgcata ccgcagccga cgccgtgtgc atgttcggga agatcggatc gtccggcgtt
      721 cccaagccga actccaggcc ctctctaggc tcgtaagcgc cggcgtcgat gccctggacc
      781 gtgcgtacga aatcggcgtc gtgcacggcg aggatctctt cgtcggtggc cgagcgagcg
      841 atcgattcat ctacatggtc gaggtcgatg agtccgagat cgcggatgtg ctcgtaggtg
      901 agaagcacgc gttcgggacg cagggggtga gacgcgccga agtcatacat gcgcgcgatg
      961 tcatcgacct tgataagcct gacggaagag gtcaactcgc tcctacggac ggtcgttggg
     1021 atccgcaccc atatagggat agcggtagtc ggtcggcggc gagaacgtct ctttgatcgt
     1081 gcgagggctg gcccaccgag cgaggttcca catcgagccg gccttgtcgt tcgttcccga
     1141 tctcctggcg cccccgaatg gttgctggcc gacgaccgca cccgtcggct tgtcgttgac
     1201 gtaaaagttt ccggccgcga agcggagtct ctctcgagcc tccacgatcg ccgcctcgtc
     1261 ggtcgagaac acggccccgg tgagcccgta tgggctcgtc gaatcgacga ggtccagcgt
     1321 ctcggcccat tcggcctcgg gatagacgaa ggtcgtgacg atcggaccga acagctcgtc
     1381 ccgcatcaag cgagcgcgag ggtcctcggt gcggaccaca gtgggctcga cgaagtaccc
     1441 ggtcgaagat tcggcatgcc caccggcgac gacctcgtat ccgggttccg accgagcctc
     1501 atcgagcgcg ttcgaatgct tggcgaaggc tcggtcgtcg atcacggccc ccatgaagtt
     1561 cgagaaatcc gcaacgtcac ccatcgcgat gttcgggacc tcgtcggcca gcctggcgcg
     1621 caacgcctcc cacaggttcg acggaacgta gacgcgcgac gctgccgagc atttctgtcc
     1681 ctggtactcg aacgagccgc ggatgatcgc ggtcgcaagg gcttccaccg aagccgatgg
     1741 gtgagcgacg atgaagtctt tcccgccggt ctcgccgacg atgcgcgggt agtggcgata
     1801 cgactcgatg ttggcgccga tcttctgcca gaaccagttg aatgtcggcg acgagccggt
     1861 gaagtggata ccggcgagct cgggtgacgc aagcacggcc tcaccgatct caggaccggg
     1921 gccgtgaacc atgttgatca cgccatcggg caagccggcg gcttcgaaca gctccatcag
     1981 gtagtgcgcg gagaacgctt gcgtcagcga cggcttccag acaaccgtgt tgcccataag
     2041 agcgggagcg gtcggaaggt tccccgcgat cgaagtgaag ttgaagggcg acaccgcgaa
     2101 cacgaagccc tcgagcggcc tgtaatcgat ctggttccac atcccgggtg ccgatatcgg
     2161 ctgctcggcg tagatccgcc cggcgtactc gacgttgaag cgccagaagt cgatcaattc
     2221 gcaagcggcg tcgatctcgg cttgatgcgc ggtcttggac tgaccgagca tcgtcgcggc
     2281 gttcacggtg tcccgccacg gtcctgcgag aagctcggcc gcgcgcagga agatcgcggc
     2341 ccggtcctgc cacggcagag ccgcccagtc cccgtgcgcc tccactgaag ccgcgatcgc
     2401 cgactcgacg tgagctgcgc tcgattggtg cacctcgccc aggacgtgct tcgtgttgtg
     2461 gggcatcacg gcttcgtaga catcgccgct gcgctcgtcc cggcccccga tcaccatcgg
     2521 gatgtcgatc cgctcctctt gcatggctcg cagacgcgtc tgcagactcc gccgctccgg
     2581 gctcccggga ccataatcgc gcaccggctc gttgtgcgca gccggaggtt gaaggatcga
     2641 gaaactactc acaagcgacc tcgcttcgtt gtcaagcctc cggctcgccc cgagccagct
     2701 cttgggaccg ctccatcgcg gcctggatcg cgttgaggaa cgcggcgcgc acccccgatt
     2761 gctcgagcac ttggatcgcc cgcgttgtcg ttccgccggg tgacgtgacc atctcgcgca
     2821 actcgaccgg atgcatgtgc gtgtctcgca ggagtttcgc cgagccgagc atcgtttgga
     2881 tgacgagttg tgtcgaaacc tcgcgcgaca aaccgaggag gatccccgct tcgatcatgg
     2941 cctcggcaag caacgcaaag taagcggggc ccgaaccgga gatcgcagtc accgcgtcga
     3001 ggtagggctc ctccacgatc acgacgcgcc cgacgtgcgc gagaacatcc gtagccagct
     3061 tgagatggtc ctcgcccgcg tggcgcccgg cggagatacc cgccatgccc tcatgcacca
     3121 ccgaaggagt gttgggcatc gcgcgcacga ccgggatcgg cgaactgagg tggctctcga
     3181 tgaaggccgt cggtatcgcg gcaactacgg aaagaacggt ttgatccggg ctgacgtggg
     3241 tgtcgatact tgcaagaagc gtttcgatgt cctgaggctt gaccgcgatg accacgatct
     3301 cgacccccgc gatcgcctga gcattatcgg tcgtagcttg caccccgtac ttcgacgcca
     3361 actcttcgag acgttccgat cgacgggcag taactgcgat ctcgtccggg gtacgccagc
     3421 ccgacgcgag gagaccggcg acgagcgcct ctcccatctt tcccgcaccg agcactgcaa
     3481 gcttcgccac agacccttct ccctctcgaa cggccctcct atcatgcagc aatgcttggc
     3541 aaggcgtggc tatgggcatc cgagaaacca cgcatccagc gcttcgtggc gcaaggaagg
     3601 atcggcaggc gagtcgtcga gcggttcgtc gccggcgaca ccctcgaaga cgcgatctcg
     3661 gtcatccggg cgttgaacga ggctgggatc ggggggatct tcgacctgct cggcgagggc
     3721 gtcaccgatc cggctggggc acaggctgcg gccgaggaat atctcgagtc gatcaagcgc
     3781 atcgaggcga ccggcgtcga cacgaccgtc acggtgaagc tgacgcagct gggcctcgcc
     3841 ttcgacaaag ggacgtgcat cgatctcctt cgggtgctgg cggcggaggc tcacgcgatc
     3901 ggcacctacg tcgagatcga catggaacag tccgattacg cgaccgacac gatcgacatc
     3961 ttcaagctcc tgcgccctga ctttccagat cttcgactcg ctctacaggc ctatctgaga
     4021 cggaccctcg tggatctcga agccctggcc ggcggccagc cgccgaaggt caggttggta
     4081 aagggggcct atgccgagcc caccgaactc gccttccaga gggatcacga gatcgaccag
     4141 caatatcgct tcatcaccga ctggctcttc gaccgaggag tcgaccccgc gatcgcaacg
     4201 cacgatgccc gactgatcca gcacgcgcgc gtctccgcag aaacgcacgg aaagagcgtg
     4261 aaggactttg aggtgcagat gctgtacggg gttcgacgcg atctgcagga aagactcgct
     4321 tcgaccggtt accgggtgcg cgtctacgta ccgttcggct cggcttggta tccgtatctg
     4381 atgcgacgga tcgcagaacg gcccgccaac ctcagactct tcctgcgcgc tcttgccggt
     4441 aaataaagaa gtaggcccca ggaaccccgt tcgtcccgct actcgtgcac ttccccacac
     4501 acgagatgcg atccgcccga agctactgag gcctacaagt cccgaggaac cctatgacga
     4561 ccttgcgacc gtccggcacc tcgggacgtg cgagaacata acgatgcaca tgcgcgcgtg
     4621 caactccgcg aatctcttga cgcgccaaca aaggtgctgg tcagggagtt gcaaactttc
     4681 ttcgcgctcc gcgtacgtgc ttctagcaag tacaagcttc cggatacggc tcccggacca
     4741 aacattcgta aagttccaag aaggcttcgg ccgatcgctt ccatgagaag acggcggcgc
     4801 gcgctcgggc cgcgcgggag aaactctccc acccgtccgg atcatcgaac aggatctgta
     4861 cccgatcggc aagacgagat ggatcgcgct cgtcgacgat gaagccggac tctccttcgg
     4921 caacgaggtg cggcaacccg ccgaccctcg ttccgatcac cggcacccca caagcttgag
     4981 cttcgagcgc ggcgagaccg aacgattcgg aatgagaact gactaagagc gcgtcggcag
     5041 cctggtagta gagcggcagg tcgatgtgcg gctgacgtcc ggcgaaaacc acgaggtcgt
     5101 cgacaccgag ctcgcgagtc agagcgcgca gcctcccggc ctcgagatgg cttagacgtc
     5161 cgctctcgcc tccgacgatg agaagccggg cggccacccc acgagctttg agctcggcca
     5221 ccacccggat ggagaggtcc atgcccttga gcttctggat ccgccccacc gacagcagca
     5281 gggggccctc acccaggccg agatcggccc gggcctcggc ccggtccccc ggcacgaaca
     5341 ggttgtggtc caccccgggc ggaaggacct tcaggcgctc ctccggggct ccgtagaggg
     5401 cctgtagatg agtgcgctcc tcctcggtcg aggccacgag cacgtcggcc ccttcgacta
     5461 cctcggcctc accggcgatt cgttcgtcgg gctcgggaga gtcgcccgga gccagccacc
     5521 ggttcttcac ccgaccgagg gtgtgatggg agtggaccag aggcacaccc cacacctgcg
     5581 cgagctctcg gccggccaaa ccggagtgcc agtagtgcga gtgaacgagg tcgtagctca
     5641 ggcgctgacc gatcgagaaa ctgcggatcc ccgacacgaa atcgctcagg tgctcttcga
     5701 gctgagactt gtcgagggac cggtgcggac cggcttcgat cgcgatcaca cggatgcctt
     5761 ctccgaaaga agcgatgcgc ggaagatcgc ccgttgcgcg cgtgaagatg tccgtcagga
     5821 ccccttgctc ggcgagagcg gtcgcgagct cgcgcacgta aaccgtcatt cctccggcat
     5881 ccccggcccc cggctcgacc aggggtgacg agtgatacgt caataccgcg agacgtctga
     5941 cgtctgtcat ctcagggatg cctcagcacg cgcacgttgt tcagcggcag ggggcgagca
     6001 ccgaactggt atttgtagcg ctccggcccc cgcaggaaat cgaaccgcct cacgccccct
     6061 tcgatcgcgt cctcgatcag ttgcgagacc aggatgtatc caggcgacag acggccgatg
     6121 gtcggatcga aagctgagtt gtagagatag aaagtgttgt cgtactggaa cgaaaacgtc
     6181 gacgcgagcg cacgatcgcc gatcgacaat gtgtcgaggc gcagccaccc gagagacgcg
     6241 aagcaatccg cgatgcgctt gaagaactct gcgatctcgg gacccatgaa ccggcctttc
     6301 gtcccctcgg cctgacggtg gaggtcgatg aacttggata ggtcctcctc gagggtgtcc
     6361 tcatccgacg agtggatgcg cgcgtctgga tggtccctac ccaatctcct gcgctttcgt
     6421 ctgagctcgt gtcgctcttt cttgtcgaga tgttcgaggt actcatccca cgaatccggg
     6481 agcgtcataa cggcggtggt ctcctcttga tccagggccc agctgaagcc gtcgcgatcg
     6541 gcgcgctcca cgagaaactc ggcgaatccg agaggcaccg gcatgttgtg agcatcgaac
     6601 tcgtcccact cgatgtcggt ctcggccagc cacttcacga gggtctccgc cactacgtcc
     6661 cggtcgtcga gcgaacaaac cggcccctga tagtcggtca agtcgatgcc cccgttgaac
     6721 cggaagatct tgcgaccatc ctccggcttc cgatagaggg gaacgatcgc ggtcaggtcc
     6781 tcacccgaat acatcgtgag cacgaacagg tccttgccgg ctccgaattg ctcccaccaa
     6841 aggcgattcc actcgggggt ggcgaagatg tggcggttcg ggtcgcgctc gaggagctcc
     6901 ttccattcgg ggagctcgaa acatctctcg gtacagtcga cgcgaacgtc taacgccatg
     6961 gtcgccggca cagtagagga caaccgaccc gagcccactt tgattccgga cgcggaaagg
     7021 gcccgccaaa gggcggaccc cctgtatctc cgatttaagg cttcttagga agccttagac
     7081 gtggtcgcga ccttgccggc cgcagaccgg gcggccttcg cgttagcgcg aacagccttc
     7141 gtcacactcg taccggcggc cttgacctgg gtacgggcgg tcttcgtctg ctgcttgagc
     7201 tgcgtagaag aaccggaagt cccggcctgg atcgccttgg tgacgctcgt cgccgcagcc
     7261 ttgacctggg tacgtgcggt cttggtctgc gtaaccgcct gcttggtcgg agcggagttg
     7321 cggatcttcg tccacaggcc gcgacctcgc ttgacggagt cgttgtacag cttggtcgtc
     7381 gacttgacgt cgggcaccga gacgctacga accttctcgg cagccagatc gccgagcccg
     7441 acgaatgcgt acaggacatc ctgagcttgc gtgtttgcca ttttcgtcac ctccttcatc
     7501 agaaattgaa atgtgggcac atggaatgac gggacatcgg agccatccga gcgcgtggtg
     7561 agcgcgctcg cgggtggggg gtagggcgtc ctggtgagga agccttgggt gaaggccgac
     7621 acgacgtccc gtc
//
LOCUS       DATMED010000035         4761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5394358, whole genome shotgun sequence.
ACCESSION   DATMED010000035 DATMED010000000
VERSION     DATMED010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5394358"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VNP73_00890"
     CDS             <1..545
                     /locus_tag="VNP73_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64506.1"
                     /translation="GMKRILSIAVMTGLLITAVSQQAVAEPTGGWTEKTVAEREFTHK
                     GQPRRYSLLHVRGHPREGSEEMYDIDITVSDTVTRTVPVRTFGFFDQEFEVSALEVPP
                     STTKIRVRVAGRYDASQPGLCITETAEGCALKIPTDPNWFNSPDSSQASLVITVTAMK
                     DDAELVNETIELPYLGQLTG"
     gene            complement(603..1199)
                     /locus_tag="VNP73_00895"
     CDS             complement(603..1199)
                     /locus_tag="VNP73_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HWL64507.1"
                     /translation="MTRRSVRAILIGVIAMLVFPFAMPSVGAAVAPDQLPLPTDDLPT
                     DILPTDDPSDDPDDLPTDLPSDLPTDLPTDLPTDLPTDAPTDVPTDVPTETPTIIPAL
                     EHTSTVTMRHAKRRFKGAVRSDEAACVPGRTVVVKRATKSGAKGIAKARTNDAGRWRL
                     KKTTAKKGRYFAKVRPLSLENSDCLPDRSKGVRLPLGR"
     gene            complement(1842..3248)
                     /gene="sufB"
                     /locus_tag="VNP73_00900"
     CDS             complement(1842..3248)
                     /gene="sufB"
                     /locus_tag="VNP73_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWL64508.1"
                     /translation="MQEENAQVRDLKGDYKYGWHDATSYDEAPQKGVSHEVIDMMSDR
                     KGEPDWMRKFRHKALDVFYRKPMPTWGADLSGIDFDDIYYYIKPMNAVKSWEDMPEDI
                     KATWDKLGIPEAEKNYLGGVSAQYESESVYHKTKEELTRRGIIFTDMDTALREHEDIV
                     KQYFGTIIPPADNKFSALNSAVWSGGSFIYVPPNTEVEIPLQAYFRINQENMGQFERT
                     LIIVDEGSWVHYVEGCSAPVYTSDSLHSAVVEIIVKRNGRCRYTTIQNWSNNVYNLVT
                     KRTAAYENAIMEWVDGNLGSKVTMKYPSVYLLGERARGEVLSIAYAGAGQHQDAGGKI
                     IHAAPNTQSAITSKSISKDGGRAGYRGLVRIEEGAEHSKSHVICDALILDDHSRSDTY
                     PYMEIEEEDAAVSHEATVSKVSDEQLFYLMSRGLDETQAMQMIVRGFIEPITKELPME
                     YAVELQRLVELQMEGSVG"
     gene            complement(3575..4354)
                     /locus_tag="VNP73_00905"
     CDS             complement(3575..4354)
                     /locus_tag="VNP73_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWL64509.1"
                     /translation="MSKSFRALAVTAHPDDMEFGAAGTLAKWADAGAEVTLCIGTDGS
                     TGTQDAALAGEELQRIRVDESERAAEVVGIKEIVWLGYRDGYVEYTLDLRKDLARVFR
                     KYRPHRFMVMDPTPVVMEFINHPDHRAIGQASLDVSMTAGTTPGHFPELIDEGFVPWR
                     GLREVWIAGPGLSPRVVDISDTIDRKIDALMCHKSQVGDNAEEIAGFVRQYTAEIGKK
                     AGFEYGESFRVLAQGPGFHAGEQEDDVDLGDLAEAPKDFRA"
     gene            complement(4351..>4761)
                     /locus_tag="VNP73_00910"
     CDS             complement(4351..>4761)
                     /locus_tag="VNP73_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWL64510.1"
                     /translation="EIGTNVLVVCAYEPPTERELEHMRADPSDPIAQWSAGKMSSGTP
                     EEFRWRLASASQAEDVLDRVRDHAAKRGVDAEVRAEKGNPAEVLVTIAEKDDVDLIAV
                     GSVGMTGAKRFMLGNVPNRISHHAPTDVLIIRTS"
BASE COUNT          875 a         1432 c         1465 g          989 t
ORIGIN      
        1 gcgggatgaa gaggattctg agtatcgcgg taatgacggg actactgatc acggctgtct
       61 cgcagcaagc ggtcgccgag ccgaccggag gatggaccga gaagacggtc gccgaacggg
      121 agttcacaca caaggggcag cccagacggt actcccttct acacgtccga ggtcacccgc
      181 gcgagggaag cgaagagatg tacgacatcg acatcacggt cagcgacacc gttacgagaa
      241 cggttccggt ccgtacattc ggattcttcg accaagagtt cgaggtcagc gcgctggaag
      301 ttcctccgtc gacgacgaag atccgtgtca gggttgcagg acgatacgac gcttcgcagc
      361 cgggactttg catcaccgaa acggctgagg gttgcgctct gaagatcccc acggatccaa
      421 actggttcaa cagtccggat tcgtcgcaag cttcactcgt catcacggtc actgcgatga
      481 aggatgacgc cgagctcgtc aacgagacga tcgagctccc ctacttaggt cagctaaccg
      541 gctaaaaagc cccgctggaa ggatcggggt cgcccttggg ggcggcccct tttcttatgt
      601 ggctaccgtc ctagcggaag acgtacgcct ttggaacgat cggggaggca gtcactgttc
      661 tcgagcgaca agggtctgac cttggcgaag tagcgacctt tcttagccgt cgtcttcttt
      721 agacgccagc ggccggcgtc gttcgttcgg gctttggcga tccccttcgc acccgacttc
      781 gtggcgcgct tcacgacgac cgtacggccc ggtacgcatg cagcctcatc cgatcggacg
      841 gcacctttga acctccgctt cgcgtgcctc atcgtgacgg tcgaggtgtg ctcaagggca
      901 ggaatgatcg tcggggtttc ggtcggcacg tcggttggta catccgtcgg cgcgtcggtg
      961 ggcaggtccg tcgggagatc ggtcgggaga tccgttggaa ggtcactcgg caggtcggtg
     1021 ggcagatcat caggatcgtc ggatggatca tcggtcggaa ggatgtccgt tggtaggtcg
     1081 tcggtcggca gaggaagttg gtcgggagcg actgcggcgc cgacgctagg catggcgaac
     1141 gggaacacga gcatggcgat aacgccaatc aggattgccc taacagacct tcttgtcacc
     1201 gcttcaactt acgttgcaag cccctggtta cgaaaggggg ttaacgaaac tgaatcgcga
     1261 tatgtcctga tttatctaga aacttcgaat cgattccggt tccgaccagt ctcgaagttt
     1321 tccttgatcc cttgcctatc ggcaaacgag aacagtgtgt agaagcgaat gtcaccgcgg
     1381 gtcggtcgaa gtctccacgg caactcatac gaccagcgct tggtaccggc cgccttcagg
     1441 acgaggtgtg accggacttc acgacatcca tcggcaaccc accggcttcg atgacgccag
     1501 aaacagtctc cgcttttcgt ccgctgaaga agcgcgagcc ggatggtcgc gccggactcg
     1561 cttgcgcgtc cgcggaagac ccttagtcgg ccggtcctga tcttggccat atggtgcggc
     1621 cgatcgatct gcgaaaagac cggtagagga tctctgtgag tcgtagtcgt ggaggggctg
     1681 ggggacggcg agccgctagg agaccctgac ccactgggag atggttgcgc taaaccggtc
     1741 ccgttcatcg ccggggcacc gatcaatgcc accacgccgc agaaccacaa cacgccgatg
     1801 gcggtggaga tggccctaag agggaaagtc acgcccagaa gttaaccgac tgagccttcc
     1861 atctgtagct cgaccaggcg ctggagctct acggcgtatt ccatcgggag ttccttggtg
     1921 atcggctcga tgaaaccgcg cacgatcatc tgcatcgcct gggtctcgtc gaggccccga
     1981 ctcatcagat agaagagctg ctcgtccgac accttcgaga cggtcgcctc gtggctgacg
     2041 gcggcatctt cctcttcgat ctccatatag gggtacgtgt cgctgcgaga gtgatcgtcg
     2101 aggatcaggg cgtcgcagat cacgtgtgac ttggagtgct cggccccctc ttcgatccgg
     2161 accaaacctc ggtagccggc ccggcccccg tccttactga tggatttgga cgtgatcgca
     2221 ctttgcgtgt tcggagccgc gtggatgatc ttgccgccgg cgtcctgatg ctggcctgcc
     2281 ccggcatagg cgatcgagag gacctcgccg cgggcacgct cccccagcag gtagacgctc
     2341 gggtatttca tcgtgacctt ggagccgagg tttccatcga cccattccat gatggcgttc
     2401 tcgtaggccg cggtccgctt ggtgaccaag ttgtagacgt tgttgctcca gttctgaatc
     2461 gtcgtgtaac ggcagcggcc gtttcgctta acgatgatct cgactacggc ggaatgcagc
     2521 gagtcagacg tgtagacggg cgcagaacag ccctcgacgt agtgaaccca cgaaccttcg
     2581 tcgacgatga tcagagtgcg ctcgaactgg cccatgttct cctgattgat gcggaagtag
     2641 gcctggagag ggatctcgac ctcggtgttc ggcggcacgt agatgaacga acctcccgac
     2701 cacaccgccg agttcagagc cgagaacttg ttgtcggcgg gcgggatgat cgtgccgaag
     2761 tactgcttga cgatgtcctc gtgctcgcgc agagccgtgt ccatgtcggt aaagatgatg
     2821 ccgcggcgcg tgagttcctc tttggtcttg tggtagacgg actcgctttc atattgggct
     2881 gagacgccgc cgaggtaatt cttctcagcc tcggggatcc caagcttgtc ccacgtcgcc
     2941 ttgatgtcct cgggcatgtc ctcccacgac ttcacggcgt tcatgggctt gatgtagtag
     3001 tagatgtcgt cgaagtcgat cccggagagg tcggcgcccc acgtcggcat cggcttccgg
     3061 tagaagacgt cgagtgcctt atggcggaac ttgcgcatcc agtcgggctc gcccttgcgg
     3121 tccgacatca tgtcgatgac ctcgtgggag acacccttct ggggggcctc gtcgtacgag
     3181 gtggcatcgt gccacccgta tttgtagtcg cccttcaggt ctctgacctg ggcgttctct
     3241 tcctggatct ttagctcttc tgctccggta gccacggtgt ccttcctgcc gggagccgtc
     3301 tctagagtct acaaccgcag gttaggcgaa cctaatcccg ccccctaacc gttctttctg
     3361 cacatccggc ccccttttta gggggagcga ttacgaagag aacaaggcga gcacccaagt
     3421 tgaccggctg ggatcaagtg cgcggaacgc atttttgctt ttcaacgaga agagcgatcg
     3481 agtcattcgg tcaaccatcc gcgagcgggc atagagcgcc gttcttttcg gaatccgccc
     3541 cctgaaaggg gggccggatg tgcagaaaga acggctaggc gcggaagtct ttgggggctt
     3601 cggctaggtc gcccagatct acgtcgtctt cctgctcgcc tgcgtggaag cctgggccct
     3661 gggcgagtac ccggaacgac tccccgtact cgaaaccggc cttcttgccg atctcggccg
     3721 tgtactgcct gacgaatccg gcgatctcct cggcgttgtc accgacctgg ctcttgtgac
     3781 acatcaacgc atcgatcttg cgatcgatcg tgtcgctgat gtccacgacc cgagggctga
     3841 gaccggggcc ggcgatccat acctctctca atccccgcca cggcacgaaa ccctcgtcga
     3901 tcagttccgg aaagtggccc ggggtcgtac cggcggtcat cgacacatcg agagatgcct
     3961 ggccgatcgc tcgatggtcc gggtgattga tgaactccat cacgaccggt gttggatcca
     4021 tgaccatgaa acggtgcgga cggtacttcc tgaaaacccg ggccaggtcc ttcctgaggt
     4081 cgagcgtgta ctcgacgtac ccgtctcggt agccgagcca cacgatctcc ttgatgccga
     4141 ccacctcggc cgcgcgctcg gactcgtcga cgcggatccg ttgtagctcc tcgccggcta
     4201 gagcagcgtc ctgggtcccc gtcgagccat cggtgccgat gcacaaggtg acctcggctc
     4261 ccgcatcggc ccacttcgcg agcgtgccgg ccgcaccgaa ctccatgtca tcggggtggg
     4321 ccgttaccgc gagcgcgcgg aaagacttgc tcacgatgtg cggatgatca agacatccgt
     4381 cggggcgtgg tgagagatgc ggttcgggac gttgcccaac atgaatcgct tcgctccagt
     4441 catccccacg cttcccaccg cgatcagatc gacgtcatcc ttctccgcga tcgtgacgag
     4501 cacctcggcg ggattgccct tctcggcgcg cacctcggcg tcgactcctc gcttggccgc
     4561 gtggtcgcgg acgcgatcga gaacgtcttc tgcctgggag gcgctggcca gacgccatct
     4621 gaactcttcc ggagtgcccg atgacatctt gccggcgctc cactgggcga tgggatcgga
     4681 tgggtcggcg cgcatgtgtt cgagctcgcg ttcagtgggt ggctcgtagg cgcatacaac
     4741 gagcacgttc gttccgatct c
//
LOCUS       DATMED010000036         7152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1946370, whole genome shotgun sequence.
ACCESSION   DATMED010000036 DATMED010000000
VERSION     DATMED010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946370"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VNP73_00915"
     CDS             complement(<1..1138)
                     /locus_tag="VNP73_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HWL64511.1"
                     /translation="MLLQRSFDDLGVPLAEVTFCVVDLETTGGSPAQNSITEVGALKV
                     RGGEKVGSFHTLVNPGEAVPAFIRLLTGIDDTMLIEAPPIEAVLPSLVEFLKDSVVVA
                     HNARFDVGFINAALERNCYPPLDNRVVDTARLAAKILSGEVRNNRLETLARHLRCAHK
                     PCHRAFADVLATTDVLHHLIERAAGYGVTTLEDLVALTATRMDGTFSKIRLADGLPRS
                     SGIYRFVGVQGQTLYVGKATDLRSRVRSYFYGDPRRKIRDMLRQTDRIEVEEYPTMLE
                     AEVAEARAIAKEIPPHNRAGKKKPGWYVKVVTRSEGGRGASKVSTARIVKPDGSVYLG
                     PYTSRTARTLIEAFRIALPLHRCSEPSRCHGCAFSEMDRCAGGRS"
     gene            1212..2969
                     /locus_tag="VNP73_00920"
     CDS             1212..2969
                     /locus_tag="VNP73_00920"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HWL64512.1"
                     /translation="MTDHPDEAELKDRLAEIERTLDSLRQDSEVAHVLLGLSGVLAEI
                     RSVEETLELAVGTVVSAFSADRCFAVTIERGEMRVLAHAGYDEGSHAHLSHIAESSEG
                     LPLIGRALSTDEPLFVPDAGDSDLIDSAGAEKRQMGAFIGLPLVRWGENLGGLGVEFD
                     RPRRFTAKDVALARGIARLLGIALVNARRFNMLASLRAFGLSVARKLSLPAVIEDVAW
                     GGVELLSGDSAKVYFLDSTHSSLVEAGGYEDERTPHQSPGRIDLHKEPWSDLLVGKAV
                     VATDLGTKEDPLSVVAAPIPGEQAAILGAVLVFFQRELAGAPEELEALNVLAGQAGMA
                     VENANRFERQRQVARSLQEGLLSTEMPELDSCTVAAVYEPASGDKDIGGDFYDVFPLS
                     DGLYGVAVGDVSGKGAEAAARTAMVKYMMRAFAISDPSPATVMANVNNALTRDLEDER
                     FATMVYGLLDPRAMKFIIARGGHPPPLLYRHETATVEALEDLEGSILGAFEDVDFVEA
                     DFTLRPGDVFLLYTDGLIEARSESGLFYGRKRIEEALKRHADEPVEELVRRLYREAAD
                     FGVVGDDTVVFALRCRGEE"
     gene            2969..3190
                     /locus_tag="VNP73_00925"
     CDS             2969..3190
                     /locus_tag="VNP73_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64513.1"
                     /translation="MYRCTACGNKTRFDVFEKKRVRTFQHFTLGGTVSIEEEEVLDHE
                     IERVVCRWCGTADGIETAESSEGSVEERT"
     gene            3187..4368
                     /locus_tag="VNP73_00930"
     CDS             3187..4368
                     /locus_tag="VNP73_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWL64514.1"
                     /translation="MTQGPLEGVRILDLTRLLPGGYATLLLADLGADVVKVEEPGKGD
                     YIRWTPPTVGEFSASHVALNRNKRSITLNLKSDAGKAAFLKLASSYDVVIESFRPGVM
                     ERLGVGWPTLRDVNPALVYCAISGYGQDGPRSQVAGHDANYIGYAGVLGIIGEEGRRP
                     AIPGVQVGDLAGGGMAAAIAILSALVRRGATGVGDFCDISMMDGAFSWLSIHAAEFQA
                     SGKEPSRELMHLSGAYPCYRVYPAADGWITVGALEPQFWAALCTALDREDLAGDAFAT
                     GERRDEVIRSLEEMFTEKTRAEWMTQLEGLDVCVGPVNTFPEAFSDEQILAREMIVES
                     PLPGVGDWKHIGNPLKLSSGAKELVRLPPPRLGEHTTELLTAAGYSEEEIEELRRGGA
                     V"
     gene            4377..5573
                     /locus_tag="VNP73_00935"
     CDS             4377..5573
                     /locus_tag="VNP73_00935"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWL64515.1"
                     /translation="MATIPQAVANELVRALGRYLRATPAVELPTRLRPFKNFHAKALS
                     TRRDELLQVLDRDEGLRARIVEWLDDKPSISANDAELLRIASERATGWEDKLAGTIAK
                     KPVRTPKTDTAPAPDGQAKKERERAEKARSEAKRIREQAQEAMRELKTELAAARAEAK
                     SERTALRTAEKRIDVVERDAAKARSDLERERRKVKADRERATQEVAEAKRELREVRRE
                     LQSRLREVARLEDKVLAQEAKGRRPHSTPKGRRKPMLVPKGRLEDAPETLDDWLNAPD
                     VHLLVDGYNATMSEGGFANLDLAGQRRRLFDEVGRLARRKSFQATIVFDGSDVVAEVR
                     RGRSPVRVQYSKPGEIADDHLIGLLETMPPNPVVLATGDRELQERGRKLGATIATSRQ
                     LLALLR"
     gene            complement(5584..6876)
                     /locus_tag="VNP73_00940"
     CDS             complement(5584..6876)
                     /locus_tag="VNP73_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL64516.1"
                     /translation="MKQTHLIALAAALLLLAWLTPPAEAAPRCFGLKVTIKGTPENDR
                     IVGTEQRDVIAAGSGNDRVLARGGDDVVCGGDGDDRLDAGGVGGRRKGSGCDVELLSG
                     GRGDDIILGDAGLNFLMGGPGRDRIHSGGNLGRCASERLRGGLGDDHLYSGTGGGRIV
                     VEGGPGNDHLVGAELDADEYSDPYGYCFDYGWCDYSAPEEPRFSGVADYSSAPSGVNV
                     NLTQGAATGAGIGSDTLVDIASIVGSKHADRLQGGQGHDELHGSGGSDVIRGEAGDDF
                     LSGDGGDDDIDGGAGLDAALFTYARALEVDLRSGNASGEGSDTLTEVEDVLAFTGHAS
                     TLTGSDAPNQLLADANSRIVGGEGNDLISTTSERGGGADLDGGAGVDYIVAGRGDDRI
                     AGGDGDDELLAGPGIDALDGGNGYDVCRDGEEVLACEA"
     gene            complement(6919..>7152)
                     /locus_tag="VNP73_00945"
     CDS             complement(6919..>7152)
                     /locus_tag="VNP73_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64517.1"
                     /translation="GIGGDSGLLLAGVLNSVIAIWIITLGVIAVRETHSFTTGRALGA
                     TALATLAIIVLAIVLVVAVLAVTIGTVAGLTGA"
BASE COUNT         1317 a         2264 c         2340 g         1231 t
ORIGIN      
        1 cggaccgtcc acccgcgcag cgatccatct cggagaacgc gcagccgtga cagcgggacg
       61 gttccgaaca gcgatgcaac ggaagcgcga tccggaacgc ctcgatcaac gtacgagcgg
      121 ttcgcgatgt gtatgggccc agataaacgg atccgtcggg cttgacgatc cttgccgtgg
      181 agacctttga ggctccccgc ccgccctcac ttcgtgtcac cactttcacg taccagcccg
      241 gcttcttctt gccggcccgg ttgtgcggcg gtatctcctt tgcgatggcc ctggcctcgg
      301 cgacctcggc ctcgagcatc gtcgggtatt cctcgacctc gatcctgtcc gtctggcgga
      361 gcatgtccct gatcttgcga cgagggtcgc cgtagaagta cgaccgcacc ctcgagcgaa
      421 ggtcggtcgc ttttccgacg taaagggtct gcccttgcac ccccacgaag cgatagatcc
      481 ccgagctgcg cggaagccca tccgccagtc ggatcttcga gaacgtgccg tccatgcgcg
      541 tcgcggttag cgcaactagg tcttcaagtg tcgtcacgcc gtagcccgcc gcccgttcga
      601 tcaaatggtg gagcacgtcg gtcgtggcaa gcacgtctgc gaacgccctg tggcaaggtt
      661 tgtgagcgca tcggagatgc cgcgccaatg tctcgagccg attgttgcga acttcgcccg
      721 acaggatctt ggccgcgagc cgagccgtgt ccacgacccg gttatccaac gggggatagc
      781 agttgcgctc cagagcggca ttgatgaagc cgacgtcgaa gcgggcgttg tgtgcgacca
      841 cgaccgagtc cttcaggaac tcgaccaggc tcggtagaac cgcctcgatc ggaggtgctt
      901 cgatcagcat cgtgtcgtcg atgcccgtca agaggcggat gaatgcggga acggcctcac
      961 cgggattcac caaggtgtgg aagctcccga ccttttcacc cccacgaacc ttgagggcac
     1021 cgacctcggt gatcgagttc tgggccggcg atccgccggt ggtctcgagg tccacgacgc
     1081 agaacgtcac ttcggcgagg ggcaccccta ggtcgtcgaa actgcgctgc agcaacatgt
     1141 tccgccacgg tatcgaacat acgttcgata tagcaagaac ccgtagggtt acaagcacta
     1201 tggcgacgga tatgaccgat cacccggacg aggcggaact caaggatcgt ttagccgaga
     1261 tcgagcgcac cctggactcg ttgcgccagg actcggaggt agcgcacgtc cttctaggtc
     1321 tctcgggtgt gctggcagag atccgctcgg tggaggaaac cctcgagcta gcggtcggca
     1381 ccgtcgtgag cgcgttctcg gccgatcgct gtttcgcggt gacgatcgaa cggggtgaga
     1441 tgcgggttct cgctcacgcc ggctatgacg agggatcgca cgcgcatctg agccacattg
     1501 cggagtcgag cgagggcctc ccgttgatcg gccgcgcctt gagcaccgat gagcctctct
     1561 tcgttcccga tgccggcgac agcgacctga tcgattcggc cggcgccgag aagcgacaga
     1621 tgggtgcctt catcggcctt ccgctcgtcc gctggggaga gaatctcgga ggcttgggcg
     1681 tcgagttcga tcgaccgagg cgcttcacgg caaaggatgt tgcccttgct cgtggcatcg
     1741 cccggcttct cggcatcgcg ctcgtgaacg cgcgccgctt caacatgctt gccagcttgc
     1801 gggccttcgg actcagcgtg gcccgcaagt taagtctgcc agcggtcatc gaggacgtcg
     1861 cgtggggtgg ggtcgagctc ttgtccggtg attccgccaa ggtgtacttc ctcgattcga
     1921 cgcattcgag cctcgtggaa gccggcggct acgaagacga acggacccca caccaatctc
     1981 ccggacgcat cgaccttcac aaagaaccgt ggagcgacct gctcgtgggg aaagccgtgg
     2041 tggcaacgga cctcgggact aaagaggatc cgctttcggt cgtggcggca ccgatccccg
     2101 gtgagcaggc ggcgatcctg ggtgccgtcc tcgtcttctt ccagcgtgag ctcgcgggtg
     2161 cgcctgagga actcgaggcg ctgaacgtcc tggccggaca agccggcatg gccgtcgaga
     2221 acgccaaccg attcgaacgt cagcggcagg tggctcgaag tcttcaggaa gggctcttgt
     2281 cgaccgagat gccggagctg gattcgtgca cggttgcagc ggtatacgaa ccggccagcg
     2341 gtgacaaaga catcggcggt gacttctacg acgtcttccc cttgtccgac ggcctctacg
     2401 gcgtcgccgt tggcgacgta tccggtaagg gggcggaggc tgccgcacgt acggcgatgg
     2461 tgaagtacat gatgcgcgcg ttcgcgatta gcgatccgtc cccggcaacc gttatggcta
     2521 acgtcaacaa cgctctaaca cgggacctcg aagatgagag gttcgccacg atggtctacg
     2581 ggctgctgga cccacgcgct atgaagttca tcatcgctcg cgggggtcac ccacctcctc
     2641 tcctgtaccg gcacgagacc gcgaccgtcg aggcgctcga agatctggag ggatcgatcc
     2701 tgggagcttt cgaggatgtc gatttcgtgg aggcagactt cacccttcgg ccgggagatg
     2761 tcttcctgct ctacaccgac ggtttgatcg aggcccgatc ggagtcgggg ctcttctacg
     2821 gacggaagag gatcgaggag gcgctgaagc gtcacgccga cgagcccgta gaggagctcg
     2881 tacgacgtct ttaccgagaa gcggccgact tcggcgtggt gggcgacgac accgtcgtgt
     2941 tcgctctgcg ttgtcggggt gaagagtgat gtatcgctgc acggcctgcg gtaacaagac
     3001 tcgcttcgat gtcttcgaga agaagcgggt gcgcacgttc caacatttca cgctcggcgg
     3061 cacggtctcg atcgaagaag aagaggtgct ggatcatgag atcgagcgcg tcgtatgccg
     3121 ttggtgcggt accgccgacg gcatcgagac ggccgagtcg tcggagggct ccgtggagga
     3181 gcggacttga cccaaggtcc gttggaaggc gtcaggatcc tcgatctgac gcgtctgctt
     3241 cccggtggtt acgcgaccct gttgctggcg gatctaggtg ccgacgtcgt gaaggtcgag
     3301 gagcccggca aaggcgacta catcaggtgg acaccaccta cggtgggcga gttctccgcc
     3361 tctcacgtcg cacttaatcg caacaagcga tcgatcacgc tcaacctgaa gtcggacgcc
     3421 ggcaaggcgg cgttcctgaa actggcgagc agttacgacg tcgtgatcga gtcgtttcgg
     3481 ccgggagtga tggagcgtct cggtgtgggg tggccgacct tgagagacgt gaacccggct
     3541 ctcgtgtact gcgcgatatc gggctacggc caagacgggc ccaggtcgca ggtcgcggga
     3601 cacgacgcga actacatcgg ttacgcgggt gtgctcggga tcatcggcga ggaaggtagg
     3661 cgtcccgcga tcccgggtgt tcaggtcggt gacctggccg gagggggcat ggccgccgcg
     3721 atcgcgatcc tgtcggccct cgtccgcagg ggcgcgacgg gcgtcggcga cttctgcgac
     3781 atctcgatga tggatggggc gttctcatgg ctgtcgatcc acgccgcgga gttccaagcg
     3841 agcggtaagg aaccctcgcg cgagctgatg catctgtcgg gggcgtatcc ctgctaccgc
     3901 gtgtatcccg ctgcggacgg atggatcacg gtgggcgcac tggagcctca attctgggcg
     3961 gccctgtgta ctgcgctcga ccgagaggac ctggccgggg atgcgttcgc gaccggcgag
     4021 cgcagggatg aagtgatccg ttctctcgaa gagatgttca cagagaagac tcgagccgag
     4081 tggatgactc aactcgaggg cctggatgtc tgcgtcggtc cggtgaacac cttccccgaa
     4141 gcgttctccg acgagcagat ccttgcccgc gagatgatcg tggagagccc gctaccgggg
     4201 gtgggtgact ggaaacacat cggcaatcca ctcaagcttt cgtccggggc gaaggagctc
     4261 gtgagattgc cgccgcccag gctcggagaa cacacgactg aactcctcac cgcagccggt
     4321 tactcggagg aagagatcga ggagttgcgc cgcggcggtg ccgtctaggc acgccgatgg
     4381 caacgatccc gcaagcggta gcgaacgagc tcgtgcgcgc cctcggtaga taccttcggg
     4441 cgacacccgc agtcgaactc cccacgcgtt tgcggccgtt caagaatttc catgccaaag
     4501 ccctatcgac gcggcgggac gagttgcttc aggtactgga tcgagacgag gggcttcggg
     4561 cgcggatcgt cgagtggctc gacgacaagc cgtcgatctc ggcgaacgac gcagagctgt
     4621 tacgcatcgc cagcgaacgc gccaccggat gggaggacaa gctcgccggc accatagcga
     4681 agaagccggt cagaacaccg aagacggaca cggctcctgc accggatggg caggcgaaga
     4741 aggaacgcga gcgggccgag aaggcccgct ccgaagcaaa gcggatccga gagcaagctc
     4801 aagaagccat gcgcgagttg aaaacggaac tggctgcggc ccgcgccgaa gcgaagtccg
     4861 agcgaacggc gctaaggact gccgagaaga ggatcgatgt cgtcgagcgc gacgcagcga
     4921 aggcgcgttc ggatctcgag cgtgagcgcc ggaaggtcaa agccgacaga gaacgggcga
     4981 cgcaagaagt ggccgaagcc aagcgcgagc tcagggaggt ccggcgcgag ttgcaaagcc
     5041 ggctacggga ggtcgctcgg ctcgaagaca aggtgctcgc gcaggaggcg aaaggccgac
     5101 ggccccactc gacgccgaaa ggccgcagga aacccatgct cgtaccgaaa ggcaggctcg
     5161 aggacgcacc cgagaccctc gatgactggc tgaacgctcc cgacgttcat cttctcgtgg
     5221 acggctacaa cgcgaccatg tccgaaggag gcttcgccaa cctcgatctc gccggacaac
     5281 gccgccggct cttcgacgag gtaggcaggc tcgcgcgccg caagagcttc caggcaacga
     5341 tcgtgttcga cgggtcggat gtcgtcgcag aggtcagaag gggtcggtca ccggttcggg
     5401 tgcagtactc gaagccgggt gagatagccg acgatcacct gatcggatta ctcgaaacga
     5461 tgccgcccaa cccggtggtg ctggctacgg gggaccggga gttgcaggag cgcggccgca
     5521 agctcggtgc cacgatcgca acgtccaggc aactcctcgc gctgctgcgc tgagtcacta
     5581 ctcttaggcc tcgcaggcca ggacctcctc tccgtcgcga caaacgtcgt agccgttccc
     5641 gccgtcgagc gcgtcgatcc ccggtcccgc cagcagttcg tcgtcgccgt caccgccggc
     5701 gatccggtcg tctccgcggc cggccacgat gtagtcgacc ccggcccccc cgtcgagatc
     5761 ggctccgccg ccacgctcgg acgtggtcga gatgaggtcg tttccttcac cgcccacgat
     5821 gcggctgttc gcgtcggcaa ggagctggtt gggggcatcg ctgccggtca gagtgcttgc
     5881 gtgaccggtg aacgcaagca catcctcgac ttcggtaagc gtgtcgctgc cctctccact
     5941 cgcgttgccc gatcgaaggt ccacctccag ggcgcgggca tacgtgaaaa gtgctgcatc
     6001 caatccggcg ccgccgtcga tgtcgtcgtc tccgccgtcg ccggagagga agtcgtcacc
     6061 cgcctccccg cggatcacgt cggacccccc actgccatgg agctcgtcgt ggccctggcc
     6121 accctgcaac cggtccgcgt gcttcgaacc gacgatcgaa gctatgtcca cgagcgtgtc
     6181 ggagccgatg ccggcccccg tggccgcgcc ttgagtgaga ttgacgttca ctcccgaggg
     6241 ggccgaggag taatcggcga cgcccgagaa ccttggctcc tccggggccg agtaatcgca
     6301 ccagccgtag tcgaagcaat agccgtacgg atccgagtac tcgtccgcgt ccagctccgc
     6361 ccccaccaga tgatcgttgc cggggcctcc ctccaccacg atccgcccac cgccagtgcc
     6421 ggagtagagg tggtcgtctc ctaagccacc ccgcaggcgc tcgctcgcac aacggcccag
     6481 gttgccgccc gaatggatgc gatcgcgacc cggtccgccc atcaggaaat tcaatcccgc
     6541 gtcgccgagg atgatgtcgt cgcctcgccc cccggacaac agttcgacgt cgcagccgct
     6601 gcccttgcgt ctcccgccca ccccacctgc gtcgaggcga tcgtcaccat cgcctccgca
     6661 gacgacgtcg tccccgccgc gagccagcac ccggtcgttg ccgctaccgg ccgcgatcac
     6721 gtcacgttgc tccgtaccca cgatgcgatc gttctcgggg gtccctttga tggtgacttt
     6781 caggccgaag caacgagggg ccgcctcggc gggaggggtg agccatgcga ggagcagcaa
     6841 ggcagctgcc agcgctatca gatgcgtctg cttcatgccc aacagacgag cggcgatggc
     6901 gaaacgttgc tcgctcggct aagcgcccgt cagtcccgca accgttccga tcgtcactgc
     6961 caggacggcg accaccagga cgatcgccag cacgatgatg gccagggtcg ccagggcggt
     7021 cgcgccgaga gctctgccgg tagtgaacga atgggtctcc cgaacggcga tcacgccgag
     7081 cgtgatgatc cagatcgcga tcacgctgtt gagcactccg gccagcaaca aacccgagtc
     7141 accaccgatg cc
//
LOCUS       DATMED010000037         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4634429, whole genome shotgun sequence.
ACCESSION   DATMED010000037 DATMED010000000
VERSION     DATMED010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4634429"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VNP73_00950"
     CDS             complement(<1..570)
                     /locus_tag="VNP73_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWL64518.1"
                     /translation="MPDFKLPDLGEGVTEGEIDKWLVKEGDVVEEDDLIVEIITDKAT
                     AEIPSPWAGVVAKIHYAEGEIVPVGTVLITIGEDTTETSSSSEEPGKTPMPATVLAAG
                     EPPPVSPGDSAAQTGNGPVKAMPPVRKLARDLGVDISAVSGSGPGGRILRPDVERMAA
                     QGVQPAVASTTAQTPGETVPAGGQRIPLRG"
     gene            complement(584..1594)
                     /locus_tag="VNP73_00955"
     CDS             complement(584..1594)
                     /locus_tag="VNP73_00955"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWL64519.1"
                     /translation="MAELTMVEALNKALHEAFSTDERVVLLGEDVAQTGGVFRVSAGL
                     LKEFGGERVIDTPLSESGIVGTAVGMSLYGMLPVVEIQFDGFVYPAFEQIVVHVARYG
                     WRTGGECPANMVIRIPFGGGVRAPELHSDSPEALFAHTPGLKVVCPTTPSDARTMLLA
                     AIAHPDPVIFMEPKRIYRAGREEVPDQMPIDLGSTNGEASSILSTARVARAGNDLVIF
                     AYGATVPLSVEAAEKMEAADGTSVRVIDLRSLYPLDVDAITNNASECGRALVVHEAPR
                     TAGIGAEVSSLIHERVMLDLEAPVGRVTGFDVPFPQFANEDDYLPSVERITRGAEKVL
                     SF"
     gene            complement(1596..>2213)
                     /locus_tag="VNP73_00960"
     CDS             complement(1596..>2213)
                     /locus_tag="VNP73_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWL64520.1"
                     /translation="TVFFCTNNQWAISTPVSRQMATRTVAEKAAAYGIPGVRIDGFDP
                     LACYKATKEALDRGRDGAGPTLIEAFTYRIKAHGTADDPSLYRDEAEIQKWIHLEPLG
                     RMASFLRRLSILDDDKDNQLRVEAKEMIAEGIRGLESIEQPGPEVMFDHVYASANPWT
                     FTEGLEEVNSVARKPEPTPPQPPSPPSAGDIDIPSESSTPGTEEG"
BASE COUNT          375 a          724 c          686 g          428 t
ORIGIN      
        1 accgcgcaga gggatgcgtt gaccgccggc gggaaccgtc tcgcccggcg tctgagccgt
       61 cgttgacgcg acggcaggtt gcacgccctg tgccgccatc ctctcgacgt ccggtctcag
      121 gatccgccca ccgggccccg agccgctgac cgcgctgatg tccacaccga gatcgcgcgc
      181 aagttttcgt acggggggca tcgccttgac cggcccgttg ccggtctgag ccgctgagtc
      241 tccgggcgat accggcggcg gttccccagc cgcaagtaca gtcgccggca tgggggtctt
      301 tccgggttcc tcggacgacg atgacgtctc cgttgtgtcc tcgccgatcg ttatgagcac
      361 cgtccctacg ggcacgatct cgccctcggc atagtggatc ttcgccacga cgcctgccca
      421 cggcgaaggg atctcggctg ttgctttgtc cgtaatgatc tcgacgatga ggtcgtcctc
      481 ctcgacgacg tcaccctcct tgaccaacca cttgtcgatc tcgccctcgg taactccttc
      541 accgaggtcg ggaagcttga aatctggcac cttgtccggc tccctagaaa ctcaggacct
      601 tctcggcgcc gcgcgtgatg cgctcgaccg agggcaggta gtcgtcttca tttgcaaact
      661 gcgggaacgg cacatcgaat ccggtcacgc ggccaacggg ggcctcgaga tcgagcatta
      721 cccgctcgtg gatgaggctg gagacctcgg cgccgatgcc cgcggtgcgc ggcgcttcgt
      781 ggacgaccag agcgcgtccg cactcagagg cattgtttgt gatggcgtcg acatcgagtg
      841 gatagagcga gcggaggtcg atcacgcgaa ccgacgttcc gtcggcagct tccattttct
      901 cagcagcttc gacgctgagc ggcacggtcg caccgtaggc gaagatcacg agatcgttgc
      961 ccgcgcgcgc tactcgtgcc gtcgatagga tcgaactagc ctcgccgttc gtgctcccga
     1021 ggtcgatggg catctgatcg ggtacctcct cgcgccccgc cctgtagatc cgcttcggtt
     1081 ccatgaagat gaccggatcg ggatgcgcga tcgcggcgag caacatcgtg cgcgcgtccg
     1141 agggggtcgt cgggcacacg acctttagtc caggcgtgtg tgcgaagagt gcctcgggtg
     1201 agtcggagtg caactcgggt gccctgacgc caccgccgaa cgggatgcga atgaccatgt
     1261 tcgccggaca ttcaccgccc gttcgccatc cgtaacgagc gacgtgcacg acgatctgct
     1321 cgaacgccgg ataaacgaac ccgtcgaact ggatctcgac aaccggcagc atcccgtaca
     1381 acgacatgcc tacagcagtc ccgacgattc cggactccga gagcggggtg tcgatcaccc
     1441 gttctccacc gaactccttg agcagtccgg cgctgaccct gaagacacca cccgtctgcg
     1501 ccacgtcctc gccgagcagc acgacccgct cgtctgttga gaaggcctcg tgcagtgcct
     1561 tgttgagagc ttcgaccatc gtgagctcag ccacgtcagc cctcctccgt gccgggagtg
     1621 ctcgactcgg acgggatatc gatgtcaccc gcggatggcg gagacggagg ctgcggcgga
     1681 gtgggttctg gtttgcgcgc aaccgagttc acttcctcga gaccctcggt gaaggtccac
     1741 gggttggccg aggcatatac gtggtcgaac atgacctcgg gaccgggctg ctcgatcgac
     1801 tcgagtcccc ggatgccctc ggcgatcatc tcctttgcct ccacccgcaa ctggttgtcc
     1861 ttgtcgtcgt cgagaatgct gagtctccga agaaaggagg ccatgcgacc gagaggttcg
     1921 aggtgtatcc acttctggat ctccgcctcg tcgcggtaga gcgatggatc gtctgccgtt
     1981 ccatgcgcct tgatgcgata ggtgaacgcc tcgatgagcg tgggcccggc cccatcgcgt
     2041 ccgcgatcca gcgcctcttt cgtggccttg tagcaagcaa gaggatcgaa tccatctatc
     2101 cgaacgcccg ggatgccgta cgcggcggcc ttctcggcca cggttctggt ggccatctgg
     2161 cgtgacaccg gagtagagat cgcccactga ttgttcgtgc agaagaacac cgt
//
LOCUS       DATMED010000038         3492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4247766, whole genome shotgun sequence.
ACCESSION   DATMED010000038 DATMED010000000
VERSION     DATMED010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4247766"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VNP73_00965"
     CDS             complement(<1..1184)
                     /locus_tag="VNP73_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64521.1"
                     /translation="MRTIHAGIQVFRRAPRSVAPLAIEGLVVAVLVLAGVFPARSGTV
                     PAGAIFPLDVYFDVKHWLAFTDGWFSFVGVVLGSLLLRAGVLAGTFVLAGEALAGSRK
                     VWLESVRLVSLAFVAFFPVAVLSFAGVAVRYAPFVWVAGVVGLPAAVILLRAAIRMDG
                     GDGGPEARGLPELTTFVSYVWLVIAGGAAVAALGSISEFLAALLIALLGPLHALFLLG
                     WRDNARTQTHSGGGLLLTSATVVALLAFGSAVLFDRYLRDAPPVSTARTPGRLLLLGG
                     ADSTSETGALLDLEVRDVGYPPGRSVLLSYRPDGRSYGAQDTHRDLVESARIISEQIE
                     RQQTSRLLGHSQASLILDRILDRDLPAPGASAVFAPAPPYPPLVSIPPPGEVGTGKAG
                     GDA"
     gene            1214..2788
                     /locus_tag="VNP73_00970"
     CDS             1214..2788
                     /locus_tag="VNP73_00970"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HWL64522.1"
                     /translation="MVVSEAPAVEERRVRDWSMVDTLCALGITVIAGVLRFARLGDPD
                     TLVFDETYYAKDACWYLFISDQVCDTDVEITQVHPPLAKWLIAIGIRLFGYDSFGWRV
                     SAAVAGTIGVLLVYLLARKLLRSTAGASIAAGLVALDFLHFVQSRISMLDIFAVTFGT
                     AAVLFVVYDRDRLIAKLRARGPDAEVDAEAAADEEEDTEPNAPVVEDHGLLDRPWRIA
                     AGAAGGAAAACKWNGGFYLLLVIVLTVTWEISARRREGKGWGGGARGFFSQELASTLL
                     WLVLLPMLVYALSYVGRLEWTDSTWYRALWDRHKYMMDFHTGLASHHSYESPAWSWIL
                     LKRPVSFFFETDSQGRYKEIFAAGNPFVWWPAILALVYIAFRWLRSRDLTSPEGVIFG
                     GFVATYLLWLPLSITGRSATFIFYLLPAIPFICLALGYIATRIGDTWEAKAAISLFWV
                     GTVGLFIFYYPLLAQTALPKPDWDRRIWIFDDCDPAEPVRTRVTVTETEDGAPVTRAT
                     TTESNENLPPPGWCWI"
     gene            complement(2785..3132)
                     /locus_tag="VNP73_00975"
     CDS             complement(2785..3132)
                     /locus_tag="VNP73_00975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWL64523.1"
                     /translation="MIETDSGLKYEEIECGDGAEAARGDTVSLHYTGTLEDGEEFDSS
                     EGKDPLTVRLGAGEVIAGFEEGLIGMHEGGTRELTIPPELGYGPSGFPPAIPPDATLV
                     FEIRLLEVDESGG"
     gene            complement(3221..>3492)
                     /locus_tag="VNP73_00980"
     CDS             complement(3221..>3492)
                     /locus_tag="VNP73_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64524.1"
                     /translation="LVALIAGSTLFVQGHFLLGLALGPAARALFETGGKVAVAVLLAV
                     LVTGGIAVWFRRRRGSGGLQSFSEACCPACLAVGLVVGRSETKPG"
BASE COUNT          651 a         1127 c         1106 g          608 t
ORIGIN      
        1 gcatcccctc cggccttgcc cgttcccacc tcgcccggag gggggatcga aacgagtggt
       61 gggtatgggg gagccggtgc gaacaccgcg ctcgctcccg gcgcagggag atcgcgatcg
      121 aggatgcggt cgaggatcaa agatgcttgc gagtgtccta ggagacggct ggtctgttgc
      181 ctctcgatct gctcagagat gatccgggcg ctctccacca agtcacggtg cgtgtcctga
      241 gcgccgtatg accgtccatc cggtctgtac gagagcagga cgcttcgtcc cggtggataa
      301 ccgacgtcgc gaacttcaag atcgaggagt gcaccggtct cagatgtgga atcggccccg
      361 ccgaggagca gtagccggcc gggagtcctg gcggtcgaga ccggtggggc atcgcgcagg
      421 taacggtcga acaacactgc ggatccgaat gccagcaaag caacaaccgt tgccgaggtc
      481 agtaaaagcc ctccgcccga gtgcgtctgt gtgcgcgcgt tgtcccgcca ccccagaagg
      541 aacagcgcgt ggagggggcc gaggagcgcg atcagaaggg cggcgaggaa ctccgagatc
      601 gaaccgagtg ccgccaccgc ggccccccct gcgatgacca gccacacgta gctcacgaag
      661 gtggtcaact cgggtaggcc gcgtgcttcg ggacccccgt cccctccatc catccggatc
      721 gccgctcgca ggaggatcac ggccgcagga agcccgacga ctccggccac ccacacgaac
      781 ggcgcgtagc gcaccgccac acccgcgaac gaaagaacgg caaccgggaa gaacgcgacg
      841 aacgccaggc tcacgagtcg gacggactcc agccagacct tcctggaccc ggcgagtgcc
      901 tcgcccgcca agacgaacgt ccccgcaagt accccagcgc gaagtaacag gctcccgagg
      961 accaccccca cgaaggagaa ccacccatcc gtgaatgcaa gccagtgctt cacatcgaaa
     1021 tagacgtcca gggggaagat cgctccggcg ggaacggttc cggagcgcgc cggaaaaacg
     1081 cccgccagca cgaggaccgc tacaactagc ccctcgatcg caagaggggc aaccgatctg
     1141 ggcgcgcgcc tgaacacctg gatcccagca tggatcgttc gcactgcgcg ggagactaac
     1201 tatgctgccg cccgtggttg taagcgaagc gccggccgtc gaggaacgca gggtcaggga
     1261 ttggtcgatg gtggacaccc tgtgtgccct ggggatcaca gtcatcgcag gcgttctgcg
     1321 cttcgcgcgg ctcggggatc ccgacacctt ggtcttcgac gagacctatt acgccaagga
     1381 cgcctgctgg tacctcttca tctcggatca ggtctgcgac accgacgtcg agatcactca
     1441 ggtgcatccg ccactggcga agtggttgat cgcgatcggc atcaggctgt tcggttacga
     1501 ctctttcgga tggagagtct cggccgccgt agcaggaacg atcggtgtcc ttttggtcta
     1561 ccttttggct cgcaagctcc tgcggtcgac cgccggagcc tcgatcgcag ccgggttggt
     1621 cgccctcgat ttcctgcatt tcgtgcagtc gcgtatctcg atgctcgaca tctttgcggt
     1681 gacgttcgga acggccgccg tcctgttcgt cgtctacgac agggaccggc tgatagccaa
     1741 gcttcgggct cgagggccgg atgcagaagt ggatgcagaa gcggcagccg acgaagagga
     1801 agacaccgaa ccgaacgccc cggtcgtcga ggaccacggg ttgctcgatc gtccgtggcg
     1861 gatcgctgcg ggggccgccg ggggcgcagc tgcggcatgc aagtggaacg gcgggttcta
     1921 cctgttgctg gtgatcgtcc tgacggtgac gtgggagata tccgcccgcc gccgggaggg
     1981 taagggctgg ggaggcggag cgcgcggctt ctttagtcag gaactcgcat cgacgctgtt
     2041 gtggttggtg ctgctaccga tgttggtcta tgcgctgtcc tatgtcggtc gtctcgagtg
     2101 gaccgatagc acttggtatc gcgcgctatg ggatcggcac aagtacatga tggacttcca
     2161 cacggggctc gcttcgcacc actcctacga gtcgccggcg tggtcgtgga tcctcttgaa
     2221 acgccccgtg tccttcttct tcgagaccga ttcgcaaggg cgttacaagg agatattcgc
     2281 agccggcaat ccgttcgtgt ggtggcctgc gatcctcgct ctcgtctaca tagcgttccg
     2341 gtggcttcga tcgcgtgacc tgacctctcc cgagggcgtg atcttcggag gattcgtcgc
     2401 cacctattta ctgtggctac ctctttcgat caccggtcgc tcggcgacgt tcatcttcta
     2461 cctgctgccg gccatcccgt tcatctgtct cgcgctcggg tacatcgcga cgcggatcgg
     2521 agacacgtgg gaggcgaagg ccgccatctc gctgttctgg gtgggaacgg tggggctctt
     2581 catcttctat tacccgctac tggcgcaaac cgcattgccg aagcctgact gggaccgtcg
     2641 gatctggatc ttcgacgatt gcgacccggc ggaaccggtg aggacccggg tcaccgtcac
     2701 ggagacggag gatggagcgc ccgtgacccg cgccacgaca actgagtcga acgaaaacct
     2761 cccgcccccg gggtggtgct ggatctagcc gcccgactcg tcaacctcta agagccttat
     2821 ctcgaagaca agggtcgcgt ccggagggat cgcgggcggg aaaccgctgg ggccgtaacc
     2881 gagttcgggt gggatcgtca gctccctcgt gccgccctcg tgcatcccga tcagtccctc
     2941 ttcgaaaccg gcgatcacct ctccggcacc gagcctgacg gtcagtgggt ccttgccctc
     3001 agaagagtcg aactcttcgc cgtcctccag ggttccggtg tagtgaagcg aaaccgtgtc
     3061 gccgcgcgcg gcctcggccc catctccgca ctcgatctct tcgtacttga gacctgagtc
     3121 cgtctcgatc acgccctgct cgcaatcggc accgttcgag cacgcgatcg cggccaggaa
     3181 cacgaggcag gcaactccga gcttcaacac gcccggcacc ctaaccgggc ttggtttcgc
     3241 tacgcccaac aaccagtccg accgccagac aggccgggca acaggcttcg ctgaagcttt
     3301 gcaggccccc agaaccacgt cgccgtcgga accagaccgc gatacctccg gtgacgagta
     3361 cagcgagcag aacggcgacg gccactttgc cgcccgtctc gaacagagcg cgagccgcag
     3421 ggcccagcgc gagacctaag aggaaatggc cctgaacgaa cagggtgctc cctgcgatca
     3481 aagccacgag ga
//
LOCUS       DATMED010000039         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3101362, whole genome shotgun sequence.
ACCESSION   DATMED010000039 DATMED010000000
VERSION     DATMED010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101362"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VNP73_00985"
     CDS             <1..795
                     /locus_tag="VNP73_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWL64525.1"
                     /translation="TNVPVAPIAGYCTDESVNEAPFYVMDFVEGPILRMRPDADPYGE
                     AQQRQIGLNVIDTLVDIHSLDPDEVGLGDLGKKEDYVARTLKRWQGQWDKGRTRDIPL
                     IDEVHERLEARIPEQGPATIVHGDYRLDNMIVTPDASGVAAVVDWELSTLGDPLADVG
                     MLLVYWGDRDAELFPLFEPATTAPGFPSLDEVKSRYSERSGRDLTLIDFYMALGLWKL
                     AIILEGVYSRYSAGQYGDKVDDNVRAFGENVMKLAEAADEAERRLG"
     gene            complement(824..1483)
                     /locus_tag="VNP73_00990"
     CDS             complement(824..1483)
                     /locus_tag="VNP73_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64526.1"
                     /translation="MPSGVRSYVRQHHVGLLALFVALGGTTAWAVDGPNPGQNTIGSE
                     DIIGNEVKSDDIGNGRIFNVDIADEIITGQKLLNGTVTGADVDENTIDNTHAAVEPVT
                     TTCDVEAETICATEESFNTDEGDDVLVTASATFYGTGMGPDAGRCRILADDVGIGHVV
                     FFGQADDAHDSALIADGVHFQALKSNLASGNHAFSLTCFEDDGDVRLGHVELIVLQYG
                     T"
     gene            complement(1551..>2562)
                     /locus_tag="VNP73_00995"
     CDS             complement(1551..>2562)
                     /locus_tag="VNP73_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64527.1"
                     /translation="SVKAGWDIALVGAGIPTATVPLGIFDPAGLPNQGGQSDHFYNAV
                     VGGLATGAIPDSEVGWFEEHGPDFLLKKLDIPILIAQGSVDTLFDLTQAHRNYQALKQ
                     NDIPLKMMWFCGGHGVCNVDSDGGAVLGDSEHVQMRRLQWFDRYLKGNEQTKTGAPFE
                     WIDQNGLWHKSPGGYPLKHARHLKGFGDGTVPVTPGITPVTGALVLALPDPAAPIKVD
                     IPAQGGEEIVGAPTLLFNYSAIGASTRLDGKAHVYGQIVDKERNVVVGNQATPIPIDF
                     DGEDHRVRVQLTRIANVAPAAGYELQIVSQSSLFDIQRAAGAVSISDLEARLPITKPR
                     KR"
BASE COUNT          428 a          825 c          844 g          465 t
ORIGIN      
        1 acgaacgtgc cggttgcgcc gatcgccgga tactgcacgg acgagagcgt gaacgaagcg
       61 cccttctacg tcatggactt cgtggaaggg ccgatccttc gtatgcgccc cgacgccgac
      121 ccctacggcg aggcccagca gcgtcagatc ggcctgaatg tgatcgacac gctggtcgac
      181 atccactcgc tcgaccccga cgaggtgggc ctcggcgact tgggcaagaa ggaggactac
      241 gtcgcgcgca cgctcaagcg ctggcagggc cagtgggaca aggggcgcac gcgcgacatc
      301 ccgctgatcg acgaggtcca cgagcgcctc gaggcacgga tccccgaaca agggccggcg
      361 acgatcgtcc acggcgacta tcgcctcgac aacatgatcg tcacgcccga cgcgagcggt
      421 gttgccgcgg tggtcgactg ggaactctcg acgctgggcg atccgctcgc cgacgtcggc
      481 atgctcctcg tctactgggg cgaccgcgac gccgagctct tcccgctctt cgagcccgca
      541 accacggcgc cgggctttcc ctcactcgac gaggtcaaga gccgctactc cgagcgctcg
      601 ggccgcgacc tcactctgat cgacttctac atggcgctcg gcctgtggaa gctcgcgatc
      661 atcctcgagg gcgtctattc ccgctactcg gcggggcagt acggcgacaa ggtcgacgac
      721 aacgttcgcg cattcggcga gaacgtcatg aagctcgccg aagcggccga cgaggccgag
      781 cgtcggctcg gctgagttac taacgcttgc gtgtctccgg ggctcaggtg ccgtactgaa
      841 gcacgatcag ttctacgtgt cccaatctga cgtctccgtc gtcttcgaaa catgtcagcg
      901 agaacgcatg gttgccgctg gcaaggttgg acttcagggc ctgaaagtgc actccatcgg
      961 cgatgagggc actgtcgtga gcatcatctg cctgaccgaa aaagacgacg tggccgatcc
     1021 ccacgtcatc ggccaagatc ctgcaacgtc ccgcgtcagg gcccatgccc gtgccgtaga
     1081 acgtggcgct ggcggtgacg agcacgtcgt cgccctcatc tgtattgaag ctctcctcgg
     1141 tcgcgcagat cgtctcggct tccacatcac acgtggtggt gaccggttcc actgcagcgt
     1201 gcgtgttgtc gatggtgttc tcgtccacgt ccgcgcccgt caccgtcccg ttcagcagct
     1261 tctgcccggt gatgatctcg tcggctatgt cgacgttgaa gatgcgcccg ttgccgatgt
     1321 cgtcggattt cacctcgttg ccgatgatgt cctcggagcc aatcgtgttt tgtcccgggt
     1381 tcggcccatc aacggcccag gctgtagtgc ctcccagggc gacgaagagc gcgagcagcc
     1441 cgacatggtg ctggcggaca taggaacgga cccctgacgg catcgtgaac cccctgcgtg
     1501 gaagacgtga acccgccgac tctctcactc gagaggacgg ctgtcaaggc ttaccgcttg
     1561 cgcggcttcg tgatcggcag gcgggcctcg aggtcggaga tcgagacggc gccggctgct
     1621 cgctggatgt cgaagaggct cgactggctc acgatctgta gctcgtagcc cgccgcgggg
     1681 gcgacgttgg cgatccgggt gagctgtacc cggacgcggt gatcctcgcc gtcaaagtcg
     1741 atcgggatcg gggtcgcctg attgccgacg accacgtttc gctccttgtc gacgatctgc
     1801 ccgtatacgt gggccttgcc gtcgagccgg gtcgaggcgc cgatggccga gtagttgaag
     1861 aggagggtgg gggcgccgac gatctcctcg ccgccctggg ccgggatatc gaccttgatc
     1921 ggggcggccg gatcggggag cgcgagcacg agagcgccgg tcaccggggt gatcccgggc
     1981 gttaccggca cggtcccgtc gccaaagccc ttgaggtggc gcgcatgctt gagcggatag
     2041 ccgccggggc tcttgtgcca cagaccgttc tggtcgatcc actcgaacgg cgcacccgtt
     2101 ttcgtctgct cgttgccctt gagataccgg tcgaaccact ggaggcggcg catctggacg
     2161 tgctcggagt cgccgagcac tgcccctccg tccgaatcga cgttgcagac accgtggccg
     2221 ccgcagaacc acatcatctt gagcgggatg tcgttctgct tcagtgcctg gtagttgcgg
     2281 tgggcctggg tgaggtcgaa gagcgtgtcc accgaaccct gcgcgatcag gatcgggatg
     2341 tcgagcttct tgaggaggaa gtccggcccg tgctcttcga accacccgac ctccgaatca
     2401 gggatcgcgc cggtcgcgag gccaccgacg accgcgttgt agaaatggtc ggactggccg
     2461 ccctggttcg gaagaccggc cggatcgaag atgcccagcg ggacggtggc ggtgggtatg
     2521 ccggcgccga cgagtgcgat gtcccaaccc gccttgaccg at
//
LOCUS       DATMED010000040         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5399497, whole genome shotgun sequence.
ACCESSION   DATMED010000040 DATMED010000000
VERSION     DATMED010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5399497"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VNP73_01000"
     CDS             <1..403
                     /locus_tag="VNP73_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWL64528.1"
                     /translation="VYTYGDVGHLDEDGYLFLSDRKIDMIVSGGVNIYPAEIESVLIT
                     HPSVADVAVFGVPNDEFGEEVKAAVQLVPGVTAGPEVENDLKSFAREKLAGYKNPRSW
                     DFHVDFPRTPVGKLQKRLLRDPYWAGKTRSI"
     gene            429..517
                     /locus_tag="VNP73_01005"
     tRNA            429..517
                     /locus_tag="VNP73_01005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:465..467,aa:Ser,seq:gga)
     gene            561..1355
                     /locus_tag="VNP73_01010"
     CDS             561..1355
                     /locus_tag="VNP73_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64529.1"
                     /translation="MKKHVRDRVSLAAAVVLVAAVVTVALVATTTSGENDASPGTDLV
                     LPTATAVPTALPAPALLGSPKPLDPGPPQRPRPGTYRYRITPPYVKDPDTTRVLRITN
                     NGSAGQTETLDGELIVQTAWRADGKYSLQHQFGQQGGVTCDWEPDIRELVFPLKAGKW
                     SSRASCNPFEGLVIEREVTSEITGAAQLIIGGQDTLVWVIRTQAHQVFRSGQEVIEQD
                     ETSRSHFSPKHGLMVRITQRRTGEDPVSRKEIDESATLELTSLDPS"
     gene            1403..1502
                     /gene="ffs"
                     /locus_tag="VNP73_01015"
     ncRNA           1403..1502
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNP73_01015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1596..3281
                     /gene="dnaX"
                     /locus_tag="VNP73_01020"
     CDS             1596..3281
                     /gene="dnaX"
                     /locus_tag="VNP73_01020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWL64530.1"
                     /translation="MTYLSLYRRWRPGTFADVLGQAHVTETLTGAIRDDRLSHAYLFA
                     GPRGTGKTSTARILAKSLNCEKGVSETPCGECTRCREIAEGTSLDVLELDAASHTSVD
                     DVREIRERIPYATAGGGRKVYIIDEAHQLSNPAFNALLKMLEEPPEHVVFVLCTTESH
                     RVPATVISRCQRFEFRRITGEILAGHLANVAQSESIEVEDTAIALIVRHARGSVRDAL
                     SLLDQAAGTAVGKVTREVILEILGEAPEDVLLDLAEAISSEDVLAVFGKVEELTALGW
                     DPRQLLVQLLEEFRALFLAHRGVVDATGVDPEHAERRRSLSQKFTGAHLEWVLQALAD
                     AQADMRLSTHPRLTLEVALARASNLEVRETQTVVARLERLERALLHGDRPAATEAPAP
                     QEPSASPPPRTTKAAAKSTPSTAPAPSSPPARKKEEAPTAPSKASTAPVEGSLAEQWE
                     AVLDRVRTQSRVTHTHLREGWAIEVTDDKLVVGFEKDFHAEQLKKRPEHIERITRVLE
                     EVFGRKLRLEPQVRAGERPGDQPGAADQADEASAEDLVRKTLGAEVVEEVSSS"
     gene            3278..>3481
                     /locus_tag="VNP73_01025"
     CDS             3278..>3481
                     /locus_tag="VNP73_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HWL64531.1"
                     /translation="MSFAPVVQRLIDELNRLPGIGPKSAQRLALHILRASQEDVDRLS
                     EAMKEMKDKVSFCRTCLNLAEGML"
BASE COUNT          701 a         1066 c         1093 g          621 t
ORIGIN      
        1 tgtctacacc tacggcgacg tcggccacct ggatgaggat ggttacctgt tcctgtccga
       61 tcggaagatc gacatgatcg tgtccggtgg cgtgaacatc tacccggccg agatcgagtc
      121 agtcctcatc acgcatccgt ccgtcgctga cgttgcggtt ttcggtgtcc ccaacgatga
      181 gttcggcgaa gaagtgaagg cggcggtaca gcttgtgccc ggagtgacgg ccgggccgga
      241 agtcgagaac gacctcaagt ccttcgcgcg cgagaaactc gccggataca agaacccgcg
      301 atcttgggat ttccacgtgg actttccgcg cacgcctgtc ggcaaactcc agaagagact
      361 gctccgcgat ccctactggg ccggcaagac ccgctccatc taagttcgct ggctaaaatc
      421 tgggctccgg aggagtcgca tagcccggcc gagtgcgcac gcttggaaag cgtgttgggg
      481 ggcaactccc acgtgggttc aaatcccacc tcctccgcga tagagacact tagcttcgct
      541 ctaaaggctg cacatagatc atgaagaagc acgttcgcga ccgggtttca cttgcagccg
      601 ccgtcgtatt ggtggccgct gtcgtgacag ttgctttggt tgctacaaca acgagcgggg
      661 agaacgacgc cagtccggga acggacctgg ttttacccac cgcgacggca gtcccgacgg
      721 ccttgcccgc gccggcgttg ctcggctccc cgaagccgtt ggaccccggt ccgccgcagc
      781 gtccgaggcc cggcacgtat cggtatcgga taacgccgcc ctacgtgaag gatcccgaca
      841 cgacgcgcgt gctgcgcata accaacaacg ggtcggctgg tcagaccgag accttggacg
      901 gcgagttaat tgtccagact gcgtggcgtg cagacggcaa gtattcgctg cagcaccaat
      961 tcggccagca gggcggggtt acttgcgact gggagcccga cattcgggag cttgtgttcc
     1021 cactcaaggc tggaaagtgg tcttcgcggg cttcgtgcaa tcccttcgaa gggttggtca
     1081 tcgaacgtga ggtcacatcg gagataaccg gtgctgcgca gttaatcatc ggcggccaag
     1141 acacgctcgt atgggtgatc cgaactcagg ctcatcaggt cttcaggtcc ggacaagagg
     1201 tcatcgaaca ggatgaaacc tcgagatctc atttctcccc caagcacgga ctcatggtgc
     1261 gcatcaccca acgacgcacg ggagaggatc cggtcagtcg caaggagatc gacgaatcgg
     1321 cgacgctgga gctgacgagt ctcgacccat cctgaccgac cggcgccgat agaatcgcgc
     1381 aggcttgacg ccatggagcg ccggtcgggt cccgcgcgac ggagggcccg cgaaccccgc
     1441 caggcccggg agggagcaac ggtagcggcg caatccgggt gccgcgggta agcctgaccg
     1501 gcgctccatc gcgttcagcc gctctaagga cggctgggct gagatcttcg agaagacgac
     1561 agggggacgt cccgagtgag tcgtagagtc ttccggtgac ctacctttct ttgtaccgcc
     1621 ggtggcggcc cggcaccttt gccgacgtgc tcggccaagc ccacgtcact gagacgctga
     1681 ccggagcgat cagggacgac cggctctccc acgcctatct gttcgccggt ccgcggggga
     1741 cgggtaagac atcgacggct cgcatcctgg ccaagtcgct caactgcgag aaaggcgttt
     1801 cagaaacacc ttgcggtgag tgcacccgtt gccgcgagat cgcggaaggc acgtcgctgg
     1861 acgtgctcga gctcgacgcc gcgtcgcaca cctcagtcga tgacgtccgc gagatccgcg
     1921 aacggatccc ttacgcaacg gccggtggcg gacggaaggt ctacatcatc gacgaggctc
     1981 accagctctc gaaccccgcg ttcaatgcct tgctgaagat gctcgaggaa ccgcccgaac
     2041 acgttgtgtt cgtgttgtgc acaacggagt cgcatcgcgt tccggccacc gtcatatcgc
     2101 gctgccaaag attcgagttc cgtcgcataa ccggagaaat tctcgccggt catctcgcaa
     2161 acgttgcgca gtcggaatcg atcgaagtcg aagacacggc cattgcgttg atcgttcgtc
     2221 acgctcgcgg ttcggtacgc gatgctctgt cgcttctcga tcaggcagcg ggaacggccg
     2281 ttggaaaagt cacgcgcgag gtgatcctcg agatcctggg tgaggcaccc gaagatgtgc
     2341 tcctcgacct tgccgaagcc atctcgtcgg aggacgtcct cgccgtattc ggcaaggtcg
     2401 aggagctcac tgccctcgga tgggatccgc ggcagctact cgttcaattg ctggaagagt
     2461 tccgagcgct gttccttgcg caccggggtg tcgtggatgc gaccggggtc gatccggaac
     2521 atgcggagcg gcgtcgatct ttgtcgcaga agttcactgg cgcacatctc gaatgggttc
     2581 ttcaggcact cgcggatgca caggccgaca tgcggttgtc gactcacccg cggctgacac
     2641 ttgaagtggc gctcgcacgc gcatcgaacc tcgaagtgcg cgaaacgcag acggtggtcg
     2701 cacgtctcga gcgattggaa cgggctctgt tacatggaga ccgcccggca gcaaccgaag
     2761 caccggcccc gcaagagccg agcgcgtctc cgccgccacg aacaactaaa gcggcggcaa
     2821 agtcgacacc atctacagca cctgcgccgt cttccccgcc ggcacgaaag aaagaagaag
     2881 ctcctacggc tccatccaag gcgtcgacgg ctcccgtcga aggatcgtta gccgaacagt
     2941 gggaagccgt tctcgatcgc gtgagaacgc agagtcgcgt tacacacacg catctgcgtg
     3001 aagggtgggc gatagaagtc accgacgaca agctcgtggt cggtttcgag aaggacttcc
     3061 acgccgaaca actaaagaag cgtcccgaac acatcgagcg catcaccagg gtcctcgaag
     3121 aggtcttcgg acgaaagctc aggctcgaac cgcaggtccg ggccggcgag cggccgggcg
     3181 accagccggg agccgccgat caggccgacg aggcgtcggc cgaggatctt gttcgtaaaa
     3241 cgttgggggc cgaggttgtc gaggaggtta gtagcagctg agcttcgctc cggtcgtcca
     3301 acgactcatc gacgagctca acaggctccc cggcatcggc ccgaagagcg ctcagcggtt
     3361 ggcgctgcac atccttcgtg cgtcgcagga agatgtcgac cgtttgtccg aagcgatgaa
     3421 ggagatgaaa gacaaggtct ccttctgccg cacgtgcttg aacttggccg aggggatgct
     3481 t
//
LOCUS       DATMED010000041         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1182021, whole genome shotgun sequence.
ACCESSION   DATMED010000041 DATMED010000000
VERSION     DATMED010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182021"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VNP73_01030"
     CDS             complement(<1..193)
                     /locus_tag="VNP73_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWL64532.1"
                     /translation="MARLFGTDGVRGVANRELTPDLALALGRAAARVLAPGGGSIVVG
                     RDTRLSGPMLEAALVAGVCS"
     gene            239..1681
                     /locus_tag="VNP73_01035"
     CDS             239..1681
                     /locus_tag="VNP73_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL64533.1"
                     /translation="MVGKTGGLITQVEIAPLDTERFEDVLAPDRYEQLVDTIVRALEL
                     FDGRRVWNINSTARGGGVAEMLQSLLAYARGAGVDTRWAVIEGEPEFFRVTKRIHNNL
                     HGSPGDGGKLGEAERKAYEKVGEANAAEMLDLISSEDVVIVHDPQPAGMIEPLQEKAA
                     VVLWRCHVGLDLPNDVARAAWNFLTPYVGKADAFVFSREQYAWESLKRERITVIPPSI
                     DAFAPKNQDLSPDQVRGILDATKLVAGGDGGSPTYETWDGTTEGVTRVTDFFDGGTPP
                     PADARLVVQVSRWDRLKDPIGVIQGFAGEVARSCDDVHLILAGPAVTAVADDPEGQDV
                     LEECRDEWSSLPPDVRDRVHLACLPMDDGDENAAIVNALQRRADVVVQKSLAEGFGLT
                     VAEAMWKARPVIASRIGGIQDQIEDEVNGLLLDDPHDTAAFGRTVVELLDNPENAEEM
                     GRKARERVRDEFLGARHLIQYVDLIEGLIA"
     gene            1691..2716
                     /gene="hemE"
                     /locus_tag="VNP73_01040"
     CDS             1691..2716
                     /gene="hemE"
                     /locus_tag="VNP73_01040"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWL64534.1"
                     /translation="MSSSFLAACRREPVDHVPVWFMRQAGRSLPEYRELRQHHDILSI
                     CRTPELAVEVTLQPVRRLGVDAAILFSDIVVPVEAIGISLDIKPGIGPVVDEPIRSVT
                     DVDRLRPLSPAEDVPYVLEAIRMLAAELDDVPLIGFGGAPFTLASYLIEGGPSRTHER
                     TKALMYSEPATWHKLMDRLAESILAYLSAQLEAGAAAVQLFDSWVGHLNVDDYRSFVL
                     PSVERIFTGLEPYGAPRIHFGVGTAHLLEAMAPAGVEVMGIDWRIGLDAARDRLGNEI
                     ALQGNLDPATCLAPWEVVEEKTAEVLEKGGGRAHIFNLGHGVLPDTDPDILSRIVDTV
                     HAWSPGD"
     gene            2709..>3441
                     /gene="hemH"
                     /locus_tag="VNP73_01045"
     CDS             2709..>3441
                     /gene="hemH"
                     /locus_tag="VNP73_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWL64535.1"
                     /translation="MTDRAVLLMSYGTPKDLDDVERYYTDIRRGNAPPPELLAELVER
                     YEAIGGRSPLLEITMRQAEGLSDRLGIPVHVGQKHSPPFIPDAFRAISEEGCERVVGL
                     VLAPHFSSMSVGDYERRSRAAAEELDWRGTLTMVESWHLEPGYVKLLAQLVEDAAAAL
                     ERPSEATVLFTAHSLPTSILESGDPYPEQLKETGAAVAEELQHTGWSIAWQSAGRTQQ
                     PWLGPDLLEVIDELADRGAKEVVVCP"
BASE COUNT          642 a         1081 c         1142 g          576 t
ORIGIN      
        1 acgagcacac ccccgcgacg agcgcggcct cgagcatggg gccggagagc cgtgtgtccc
       61 ggccgaccac gatcgaacct cccccggggg ccagaacgcg agcggcggct cgacctaggg
      121 caagagcgag gtccggcgtc agctcgcggt tggctactcc gcgtactccg tcggttccga
      181 ataggcgcgc caccgggcaa tcctcccagg tcgtcggtcg gtcgggcaag ataacggggt
      241 ggtcgggaaa acagggggac tcataacgca ggtggagatc gcgccgctcg ataccgagcg
      301 attcgaggat gtcttggccc cggatcggta cgaacagctc gtcgacacga tcgttcgagc
      361 tctcgagctc ttcgacggca gacgcgtctg gaacatcaac tcgaccgcgc gcgggggcgg
      421 cgtggccgag atgctccagt cactgctcgc gtacgcgcgc ggcgcgggtg tagacacacg
      481 ttgggctgtg atcgaagggg aaccggagtt cttccgggtg acgaagcgga tccacaacaa
      541 cctccacggc tcccccggag atgggggaaa gctcggcgag gcagagcgca aagcgtacga
      601 gaaggttggt gaggccaacg ctgccgagat gctcgacctc atctcgtcgg aagatgtcgt
      661 catcgtgcac gacccgcaac cggccggcat gatcgagccc ttacaagaga aggcggcagt
      721 cgttctatgg cgttgccacg tcgggctcga ccttccgaac gacgtggcgc gggctgcatg
      781 gaacttcctc accccgtacg tcggcaaggc agatgcgttc gtcttctctc gcgagcagta
      841 cgcgtgggag agcctgaagc gagaacggat aacggtcatc cctccctcga tcgatgcatt
      901 cgctccgaag aatcaggacc tgagccccga ccaggtgcgc ggcatcctgg atgcgacaaa
      961 gctcgttgcg ggtggagacg gtggctcgcc tacatacgag acgtgggacg gaacgacgga
     1021 gggggtcacc cgggtgacgg atttcttcga cggaggaact cccccaccgg ccgacgcacg
     1081 gctcgtggtg caggtgtcgc gctgggaccg tctcaaggat ccgatcggtg tcatccaggg
     1141 tttcgccggc gaggtggccc ggtcgtgtga cgacgttcat ctcatcctcg ccggtccggc
     1201 ggtgactgcg gtcgccgacg acccggaggg ccaggacgtt ctggaggagt gcagggacga
     1261 atggtcgtcg ttgcctccag acgttcggga ccgcgttcac ctggcgtgtc tgccgatgga
     1321 cgacggtgat gagaacgcag cgatcgtcaa cgctcttcag aggcgtgcgg atgtcgtcgt
     1381 tcagaagagc ctcgcggagg gattcggtct aacggtggcc gaggcgatgt ggaaagctcg
     1441 gcccgtgatc gccagccgga tcggggggat ccaggatcag atcgaggatg aagtgaacgg
     1501 actgttgctc gatgatcccc acgacacggc ggcgttcggc aggacggtcg tcgagctcct
     1561 ggacaacccc gagaacgccg aagagatggg ccggaaagcc cgcgaacggg tccgggacga
     1621 gttcctcggc gcgcgccact tgatccagta cgtcgatctg atcgaaggac tcatcgccta
     1681 gggttcatcc gtgtcatcga gtttcctcgc ggcgtgcagg cgcgagcccg tcgaccacgt
     1741 ccccgtttgg ttcatgcgcc aggcgggacg cagcctgccc gaataccgag agttacggca
     1801 gcatcacgac atcctgagca tctgcaggac cccggaattg gctgtggaag tcacccttca
     1861 acccgttcgg cggttggggg tcgacgccgc gatcctcttc tcggacatcg tcgtcccggt
     1921 cgaagcgatt ggcatcagcc tcgacatcaa gccgggcatc ggcccggtcg tcgacgaacc
     1981 gatccgctcg gtgaccgatg tcgaccgcct gaggccgttg agcccggcgg aagacgttcc
     2041 ctacgttctc gaagcgatac gcatgctggc cgcagagctc gacgacgtgc cgttgatcgg
     2101 gttcgggggc gcgccgttca ccctcgccag ttacctgatc gaaggcggcc cgtctcgtac
     2161 gcatgagcgg accaaagctc tgatgtattc ggaacctgcc acctggcaca agttgatgga
     2221 tcggcttgcc gaatcgatcc tcgcttacct ctcggcacaa ctcgaagcgg gggccgcggc
     2281 agtgcaactc ttcgactcct gggtcgggca cctgaacgtg gacgactatc ggagcttcgt
     2341 tctgccgtcc gtcgagcgca tcttcacagg actcgaacca tacggagcgc cccgcatcca
     2401 cttcggtgtc ggcacggcac atcttctgga agcgatggcg cccgcggggg tcgaagtcat
     2461 gggcatcgat tggcgcatcg gtctcgacgc cgcacgcgat cgcctcggta acgagatcgc
     2521 acttcaggga aacctcgacc cggctacctg cctcgcgccc tgggaggtcg tcgaggagaa
     2581 aaccgcagag gtgttggaga agggtggggg ccgagcacac atcttcaatc tcggacacgg
     2641 tgtccttccg gacaccgatc cggacatcct gagcaggatc gtagacaccg tgcacgcatg
     2701 gagtcccggt gactgacaga gccgtgctgc tcatgtctta cggcacgccg aaggacctcg
     2761 acgatgtcga gcgctactac acggacatcc gacgcggcaa cgcaccccca ccggaactcc
     2821 tcgcagaact cgtcgagcgc tacgaagcga tcggaggacg ctccccactg cttgagatca
     2881 cgatgcgcca agccgaaggt ctcagcgacc ggctgggcat ccccgtgcac gtcgggcaga
     2941 aacactctcc accgttcatc cccgacgcct tccgagcgat ctcggaagaa ggttgtgagc
     3001 gcgtggtcgg tctcgtgctg gccccgcact tctcctccat gagtgtcggg gattacgagc
     3061 gtcgcagcag agcggccgcc gaagagctcg actggcgggg gacgctgacc atggtcgagt
     3121 cgtggcacct cgaacccggt tacgtgaagc tcctcgcgca actggtcgaa gacgcggcgg
     3181 cggcgttgga acggccatcg gaggcaacgg ttctcttcac ggcgcacagc cttccgacgt
     3241 cgatcctcga gtcgggcgac ccctaccccg agcaactgaa ggaaacgggg gccgcggtgg
     3301 ccgaagagct ccagcacacc gggtggtcga tcgcatggca gtcggccggg cggacccagc
     3361 agccgtggct gggccccgat ctgctcgaag tcatcgacga gctcgccgat cgcggagcga
     3421 aagaagtcgt cgtatgcccg t
//
LOCUS       DATMED010000042         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2339708, whole genome shotgun sequence.
ACCESSION   DATMED010000042 DATMED010000000
VERSION     DATMED010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339708"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VNP73_01050"
     CDS             complement(<1..553)
                     /locus_tag="VNP73_01050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWL64536.1"
                     /translation="MTAKADFGVVGAGIVGCLAAWELARRGHRVTVHEAEQIGAGASG
                     GLGSRGVRANGRDPRELAVAAEALGMWPQLGETLGAGIGWHQTGHLLLTERPDDREEL
                     ANIAGRQSDAGIETTVVEGAELKALEPGLAPEVLAAAWCPGDGVADHTATTQIVATAA
                     TEAGVEFRLKNPVSSLDAIDAVDG"
     gene            607..>2044
                     /locus_tag="VNP73_01055"
     CDS             607..>2044
                     /locus_tag="VNP73_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HWL64537.1"
                     /translation="MTKRSLFVLALVAVLLSAFVPVGRTDSARPMLVRVHPSNALEAE
                     FLMSHFDETHNHGRGQIELLLWPGDAAELDALDIDYEVVVEDLFAHDLAQGGTAAAEV
                     QVLPGPDRDDYRRLVDYNAEMADLEKKNPDLVKLFQFPQPTLEGRPVFGVEIAADVKA
                     VDDGRPIFYIDGVHHAREWPASEYTMLFVHHLVEKFGKDPKITSLLKKARVIAVPIVN
                     VDGFDYSRESVTSVNQPARDYTDNLGAGNGFEGYWRKNRRSLTGATAPGLQRNPDAFG
                     VDPNRNYSYLWGDSNGGSSSLQLDATYRGEAPFSEPETQNVRDLILSRPVTGVITNHT
                     YQASVLRAGGGKAPEDAILAAIGDKMAKILGYTNAPTVGYPTTGTTDDWSYAAMGALG
                     FTIEHGRLGFHPAYDLEVGKFWKQHMDAFMVMFGVSASPRYHSVIEGKVAGGPAELTI
                     TKTFDTELSPGNPTGEESVVEKIKMTLTT"
BASE COUNT          373 a          696 c          619 g          356 t
ORIGIN      
        1 tcccatcgac agcatcgatc gcgtcgagag acgagacggg gttcttcaac cgaaactcca
       61 ctccggcttc ggttgcagca gtcgcgacga tctgggtcgt cgcggtgtga tccgcaacgc
      121 cgtcgccggg acaccacgcg gcggcgagca cttccggcgc gagcccgggt tcgagagcct
      181 tcaactcggc cccctccacg accgtggttt cgatgcccgc gtcgctctgc cgtcccgcta
      241 tgttggcgag ctcctcgcga tcgtcggggc gctcggtcag caacaggtga cccgtctggt
      301 gccagccgat cccagctccc agggtctcgc ccagctgggg ccacatcccc agcgcctccg
      361 ccgcgaccgc caactcgcgg gggtcgcggc cgttcgccct gaccccccgg ctgcccaacc
      421 cgccggaggc cccggcccct atttgctctg cctcgtgaac ggtcacacga tggcctcgcc
      481 gggctagttc ccacgcggcc aggcatccga cgatgccggc tcccaccacc ccgaaatcgg
      541 ctttggcggt cacgcacggc actctaggcg gcagggatac gaggcggcct gccgaatcat
      601 cccctcatga ctaaaagatc attgttcgtc ctcgccctgg ttgcagttct gctttcggcc
      661 ttcgtccccg tcggccgcac cgattccgct cgcccgatgc tcgttcgcgt gcatccgtcg
      721 aacgctctcg aggctgagtt cttgatgagt catttcgacg agacccacaa tcacgggcgc
      781 ggccagatcg agctcctgtt gtggcccggc gacgcggccg agttggatgc tctcgacatc
      841 gattacgaag ttgtggtcga ggatctcttc gcccacgacc tcgcgcaggg gggcaccgct
      901 gccgccgagg tgcaggtcct ccccggcccg gatcgcgacg actacaggcg cctcgtcgac
      961 tacaacgccg agatggcgga tctcgagaag aagaaccccg accttgtgaa actgttccag
     1021 ttcccgcaac ccacgctcga aggccgtcct gtattcggcg tcgagatcgc cgccgatgtg
     1081 aaggccgttg acgatggtcg accgatcttc tacatcgatg gggttcacca cgcgcgcgag
     1141 tggccggcgt cggagtacac gatgctgttc gtccaccacc tcgtcgagaa gttcggcaag
     1201 gatcccaaga tcacatcttt attgaagaaa gcccgagtca tcgcggtgcc gatcgtcaac
     1261 gtcgacggct tcgattactc acgtgagtcg gtcacatccg tcaaccaacc tgcgcgcgac
     1321 tacacggaca acctgggggc gggaaacgga ttcgaaggct actggcgcaa gaaccgccgg
     1381 tccctgacag gcgcgacggc accgggttta cagcggaacc cggacgcgtt cggggtcgat
     1441 ccgaacagga actactccta tctatggggt gactcgaacg gaggctcctc cagtttgcag
     1501 ctcgacgcga cctaccgcgg cgaagcaccg ttctcggaac ccgagaccca gaacgtgcgt
     1561 gacctgatcc tcagtcgtcc ggtgaccgga gtgatcacga accacaccta ccaggcttcc
     1621 gtgttgcggg cgggtggcgg gaaggctcca gaggacgcga tcctggccgc catcggcgac
     1681 aagatggcaa agatcctggg ctacaccaac gcaccgacgg tgggctatcc gacaacgggc
     1741 acgaccgacg actggtctta cgccgcgatg ggggcgctcg ggttcacgat cgagcacggc
     1801 aggctcggct tccaccccgc gtacgacctc gaggtcggca agttctggaa gcagcacatg
     1861 gatgcgttca tggtgatgtt cggcgtctct gcgtctccgc gctaccactc cgtgatcgag
     1921 ggcaaggtcg cagggggccc ggccgagctg accatcacca agacattcga tacagagttg
     1981 tcccccggca acccgacggg cgaagaatcc gtcgtcgaga agatcaagat gacgctgaca
     2041 acca
//
LOCUS       DATMED010000043         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3485800, whole genome shotgun sequence.
ACCESSION   DATMED010000043 DATMED010000000
VERSION     DATMED010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485800"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VNP73_01060"
     CDS             complement(<1..1046)
                     /locus_tag="VNP73_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWL64538.1"
                     /translation="MDINKLTLKSQEALNNAQSIAKELNHQQVESAHLLTALLREPEG
                     VVYPLLQKLGVAPRGVVSRLEAVLDSLPKVYGEIEIYLGQSLKKVLDSAFKEMEELRD
                     SYVSTEHLLLAILAQKDDTAAVLTEAGIDKAGVLNALTEIRGSHRVTDQTPEDKYQAL
                     ERFGRDLTEAARRGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     ANRIVAGDVPESLKNKRLISLDIGSMVAGSKYRGEFEERLKAVLNEIVASEGEVITFV
                     DELHTVVGAGAAEGAVDAGNMIKPMLARGELRMVGATTLDEYRKHVEKDPALERRFQP
                     VLVGEPSVADTIAIL"
     gene            complement(1061..1531)
                     /locus_tag="VNP73_01065"
     CDS             complement(1061..1531)
                     /locus_tag="VNP73_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWL64539.1"
                     /translation="MADRHRPTDPDRATFVISIAAELAGVHPQTLRVYERKGLVQPQR
                     TEGNMRRYSQNDIERLRRIQELTQEGLNLAGVMRVIELERELARMGRLYERALADVAV
                     IQDQIQELMTARDHNAMVPLRDMRRIRRAMKADIVDQAGRRRTWPAPPVGGPTE"
     gene            complement(1534..>2455)
                     /locus_tag="VNP73_01070"
     CDS             complement(1534..>2455)
                     /locus_tag="VNP73_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWL64540.1"
                     /translation="DFYKVLGVSEKATKDEIKKAYRRLAQKYHPDANKGDAAAETRFK
                     ELSEAHSVLSSDQKRKEYDEFRRLAAAGGERWYGFSPGGGQGNVRVNIGDLFGDEGGL
                     FEDLLGGGFGFRPNGPGKGADMEAETTLTFEQAIGGATITLPQGTKVNIPPGVGNGAR
                     IRVAGKGSPGPNGGAPGDLFVRVNVEAHPVFTRSAKGDLMITVPVTFTEAVLGAQIEV
                     PTLDGRVKVKVPAGTPNGKTFRVRGRGPIKTKGGKGDLLVKIEVQVPQKLAKKEKQLL
                     EEFADVHKEDPRKHLEEYLKRSGSGTREAV"
BASE COUNT          384 a          816 c          728 g          527 t
ORIGIN      
        1 aggatcgcga tcgtgtcggc gacggacggc tcacccacca gcaccggttg gaacctgcgc
       61 tccagagcgg ggtccttctc gacgtgtttc cggtactcgt cgagcgtggt ggcaccgacc
      121 atgcggagct cgccgcgcgc cagcatcggc ttgatcatgt tgccggcgtc gacagctccc
      181 tccgctgcac ccgctccgac gaccgtgtgc agctcgtcga cgaacgtgat gacttccccc
      241 tcggaagcaa ctatctcgtt aaggactgcc ttcaatcgtt cctcgaactc gccgcggtac
      301 ttcgatccgg cgaccatgga gccgatgtcg agcgagataa gacgcttgtt cttgagcgac
      361 tcgggcacgt cgccggcgac gatgcggttc gccagccctt cgacgatcgc cgtcttgccg
      421 acgcctggct ccccgatcag gaccggattg ttcttcgtcc tgcgcgagag aacctggatc
      481 acgcgccgga tctcttcgtc tcgtccgatg acgggatcga gctttcctcg gcgtgcggct
      541 tcggtcagat cgcgaccgaa gcgctccagc gcctggtact tgtcctcggg cgtctggtcc
      601 gtgacgcgat gcgagccgcg gatctccgtg agcgcgttga gcacaccggc cttgtcgatg
      661 ccggcctcgg tgagaacggc tgcggtgtcg tctttctggg ccaggatcgc cagcagcagg
      721 tgctcagtcg aaacatatga gtccctgagc tcctccatct ctttgaatgc cgagtcgagg
      781 accttcttca gcgactggcc gagatagatc tcgatctcgc cgtacacctt cggaagggaa
      841 tcgagaactg cctccagccg cgaaacgacc ccccgcggtg ccacacccag cttctgtaga
      901 agcggataca ccacgccctc gggttcgcgc agcaacgcag tcagcaggtg agcgctctcg
      961 acctgctggt gattgagttc ctttgcgatg ctctgtgcgt tgttgagtgc ctcttgggac
     1021 ttaagtgtca gcttgtttat gtccatcgtt ctgcctcttg ctattcggtg gggccgccca
     1081 cggggggtgc aggccaggtg cggcggcgac ccgcctggtc cacgatgtct gccttcatcg
     1141 cgcgccggat gcggcgcatg tcccggagcg gaaccatcgc gttgtgatct cgagcggtca
     1201 tcaactcttg gatctggtcc tggatgaccg caacgtctgc cagcgcgcgc tcgtagagcc
     1261 tccccatcct cgcgagctcg cgctctagtt cgatcactcg catgacgccg gcgagattca
     1321 agccctcctg tgtcagttcc tgtatacggc gcagccgctc gatgtcgttc tgggagtacc
     1381 gccgcatgtt cccctcggtc ctctgcggct gcaccaagcc ctttcgttcg tacacgcgca
     1441 atgtctgcgg atgtacgccg gccagctcgg ccgcgatcga gatcacgaat gtcgctcgat
     1501 ctggatcggt cggtcggtgt cggtcggcca tggctagacg gcctccctag tgccggaacc
     1561 ggagcgcttc aggtactcct cgaggtgctt ccgaggatcc tccttgtgga cgtcggcgaa
     1621 ctcctcgagc aactgcttct ccttcttggc cagcttctgc ggcacttgca cctcgatctt
     1681 cacgaggaga tcaccttttc cacccttggt cttgatcggc ccacgaccgc ggacgcgaaa
     1741 cgtcttaccg ttgggggtgc cggccggcac cttgaccttg acccttccgt cgagagtggg
     1801 tacctcgatc tgcgcgccga gcacggcctc tgtaaatgtc accggaacgg tgatcatcag
     1861 gtcgcccttg gcgctgcgcg tgaaaacggg atgggcctcg acgttgaccc gaacgaagag
     1921 gtccccgggt gcacctccat tgggccccgg actccctttg ccggccacgc ggatccgagc
     1981 tccgttaccg acgcccggag ggatgttcac cttcgttccc tgtgggagcg tgatcgtcgc
     2041 gcccccgatc gcttgttcga aggtgagcgt cgtctccgcc tccatgtccg cgcctttgcc
     2101 aggaccgttc ggccggaagc cgaagccacc acccagaaga tcctcgaaca gaccaccctc
     2161 gtcgccgaaa aggtctccga tattcacccg aacgttgcct tgtcccccac cgggagagaa
     2221 cccgtaccag cgctctccac cggctgctgc aaggcggcgg aactcgtcgt attccttgcg
     2281 cttctgatcg ctagagagaa cggagtgggc ttcggagagc tccttgaaac gcgtctcagc
     2341 cgcggcgtct cccttgttgg cgtcggggtg gtacttctga gccaacctgc ggtacgcctt
     2401 tttgatctcg tccttggtcg ccttctcgga gacgccgagg accttataga agtcc
//
LOCUS       DATMED010000044         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3876389, whole genome shotgun sequence.
ACCESSION   DATMED010000044 DATMED010000000
VERSION     DATMED010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3876389"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VNP73_01075"
     CDS             complement(<1..1337)
                     /locus_tag="VNP73_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HWL64541.1"
                     /translation="MKPRVLARIGAGLSAALIAGLLGLAPATASPNGVVVSEFRFRGP
                     LGGNDEFVELLNTSGVDVDISGYKFIGCSASGPTGTRATVPAGVTLEPGEHYLFTNTA
                     ANGYSGTVPGDTNYTTGVSETGGARLVTAADALIDAWGSTSTIAECREGAGLTLPTTN
                     GENAFQRIAGETQDADDNAADFAGPQTADPDNWGEDPGDPPGTISKIHDVQGAGATTP
                     VNGQIVTIEGIVVGHDDEDGFNFERNFPEDRGLFVQEEPSDQDANPATSEGIFVGFVD
                     NVLDYPLGSVVKVEGQAKEKFGLTMIAETFGQEPTRVGTSTVPLPEPATIDVTQAESQ
                     SESSKPYYETLEAMRVRLADGTANSGGTTKFDELFLTPGTDRDRVFRTESEPALLATD
                     ADAGAGDPQDPYEDPDGSSTVVNADLFDTVQNVVGPLAFSFNHFKIMVQADVVP"
     gene            complement(1489..>2345)
                     /gene="serS"
                     /locus_tag="VNP73_01080"
     CDS             complement(1489..>2345)
                     /gene="serS"
                     /locus_tag="VNP73_01080"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWL64542.1"
                     /translation="EHVELGETLGIFDSDRAAKASGSRFVYLTGPGVILELALVRFAI
                     DYLTDHRFTPVVPPVLVRRRAMYGTGFLPAEEHEFYVSERDELFLTGTSEVALASMHA
                     DEILKESDLPIRYAGYSPCFRREAGSYGKDTKGLIRVHQFDKVEQFSFCHPDDSWDEF
                     QAIRANQERLLQTLEIPYQVLVMCAGDLGSAAAKKVDNEAWLPGAGRYLELTSASNTT
                     DYQARRLSIRFRGAGTRLVHTLNGTACAVGRTIVALLENHQRGDGSVAIPEALQPYTG
                     FKELNPVR"
BASE COUNT          417 a          749 c          743 g          436 t
ORIGIN      
        1 ggaacgacat cggcctgcac catgatcttg aagtggttga acgaaaatgc taacggcccc
       61 accacgttct gcacggtgtc gaagagatcc gcgttgacca cggtgctcga cccgtcggga
      121 tcctcgtaag gatcctgggg atcgcccgct cccgcgtcgg catcggtcgc gaggagcgca
      181 ggctcgctct cggtgcggaa cacgcgatcg cggtcggttc ccggggtcag gaagagttcg
      241 tcgaacttgg tcgtcccacc ggaatttgcg gtcccatcgg cgagccgcac gcgcatggcc
      301 tcgagtgtct cgtagtaggg cttgctcgat tcggactgcg actcggcctg ggtcacgtcg
      361 atagttgcgg gctcggggag gggcacggtc gacgtcccta ccctggtggg ctcctgaccg
      421 aaggtctcgg cgatcatggt aaggccgaac ttctccttgg cctgaccctc gactttcact
      481 accgagccca ggggatagtc gagcacgttg tcgacgaaac caacgaagat gccctcggag
      541 gtcgccggat tggcatcctg atcggagggc tcctcctgca cgaagaggcc gcgatcctcg
      601 gggaaattcc gctcgaagtt gaagccgtcc tcgtcgtcat gtccgacgac gatgccctcg
      661 atggtcacga tctggccgtt taccggagtg gtcgcgcccg ctccctggac atcgtggatc
      721 ttggaaatgg tgccgggagg atctcccgga tcctcgcccc agttgtcagg atccgcggtc
      781 tgcggtcctg caaagtccgc ggcgttgtca tctgcgtctt gcgtttcgcc ggcgatgcgc
      841 tggaacgcgt tctcaccatt cgtggtcggc aacgtgaggc ctgcgccttc acgacactcg
      901 gcgatcgtcg acgtgctgcc ccacgcgtcg atcaacgcgt cggcggccgt tacgaggcgc
      961 gccccaccgg tctcacttac gcccgtcgtg tagttggtgt cgcccgggac ggtgcccgag
     1021 tagccgttcg ccgctgtgtt ggtgaacagg tagtgctcgc ccggctcgag ggttactccc
     1081 gcaggcacgg tggcccgcgt tccggtcggc ccgcttgcgc tacagccgat gaacttgtaa
     1141 ccggagatgt cgacgtcaac gcctgacgtg ttcagcagtt cgacgaactc gtcgttgcca
     1201 cccaaaggcc ccctgaaacg aaactccgag acgacgacac cgttaggcga cgccgttgcc
     1261 ggcgcgagcc ccaacaatcc cgcgatgagc gccgccgaca ggcccgcgcc gatgcgagcc
     1321 aaaacacgtg gcttcactga tccctcccct tgtttgatgg acaccaccct gaacggcgca
     1381 ggggaagctg cgcatgtggg gtgcccattc atcctgattc gcgctcgaca ttagcccacc
     1441 gggacgggat cacaagcgat acgacgaaca gttgctacga ggttcacgtc atcggacggg
     1501 gttgagctcc ttgaaaccgg tatagggctg aagcgcctcc ggaatggcta cgctgccgtc
     1561 gcccctctga tggttctcga gcaaggcaac tatggtccgc ccgacggcac aggccgtccc
     1621 gttcaaggtg tgaacgagcc tcgtgcccgc cccgcgaaac cggatgctca ggcgacgggc
     1681 ctggtagtcg gtggtgttgg acgccgaagt cagctcgagg tagcgcccgg cccccggaag
     1741 ccacgcctcg ttgtcgacct tcttggccgc cgccgagccg aggtcgccgg cgcacatcac
     1801 caggacctga tacgggatct cgagagtctg caggaggcgc tcctgattgg cgcggatcgc
     1861 ctggaactcg tcccaggaat cgtcgggatg acagaacgag aactgctcga ccttgtcgaa
     1921 ttggtggacg cgaattagcc cctttgtgtc cttcccgtag cttcccgcct cgcgtctgaa
     1981 gcacggcgag taacccgcgt accgaatcgg cagatcgctc tctttgagga tctcgtccgc
     2041 atgcatcgac gcgagggcaa cctcagacgt gccggtgagg aacagctcat cgcgctcgct
     2101 cacatagaac tcgtgttcct ctgccggcag gaagccggtg ccgtacatcg cgcgccgacg
     2161 gacgaggacc gggggaacaa ccggagtaaa gcgatgatcg gtcaagtagt cgatcgcaaa
     2221 gcgaaccagt gccaactcga gaatgacgcc ggggccggtc aggtatacga agcgactgcc
     2281 ggatgcctta gcggcccggt cggagtcgaa gatgccgagc gtctcgccga gctcgacgtg
     2341 ctcct
//
LOCUS       DATMED010000045         9541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1954600, whole genome shotgun sequence.
ACCESSION   DATMED010000045 DATMED010000000
VERSION     DATMED010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954600"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VNP73_01085"
     CDS             complement(<1..696)
                     /locus_tag="VNP73_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWL64543.1"
                     /translation="MPAQTITEKILAAHAGKDRVAPGEIVMCKVDLAMANDVTAPPAV
                     DSFRKMGASKVWDRSKVALVASHFVPAKDIASAGLMSVMREFALEQDIEHFFDVGRGG
                     IEHILLPEEALTLPGDVVIGADSHSCTYGALGCFSTGVGSTDIAAVWATGEIWLRVPE
                     SMKIVYEGRPGPWVVGKDLILTVIARIGDDGASYMAMEHTGSALQHLSIDSRLTLTNM
                     AIEAGAKSGIIPPD"
     gene            complement(696..2264)
                     /locus_tag="VNP73_01090"
     CDS             complement(696..2264)
                     /locus_tag="VNP73_01090"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWL64544.1"
                     /translation="MGGGGKDGDPGVVRIFDTTLRDGEQSPGISLNLKEKVEIAEQLA
                     RLGVDIIEAGFPISSEGEKEAVRAIAENVRGPVICGLARVDAIDIDTAAEAIAPAQRG
                     RIHTFIATSEIHMAKKLQMTPDQVLAAAVGGVARARRYVDDVEFSCEDATRSDPEFCA
                     KVFKAAADAGATTLNLPDTVGYALPQEFADLLRFLMGEVDREVVWSVHTHDDLGLAVA
                     NALAGIEVGARQVEVAINGIGERAGNCSLEEIVMALRTRKAEDRPDTAIHTKEIARTS
                     RMVSMLTGYPVPANKAIVGANAFAHEAGIHQHGVMVERTTYEIMDPLDVGLEGSKIVL
                     GKHSGRHAFSKALEDLGFNLSREDMQRAFDRFKALADRKIRITDVDLEAIVVDEMAGD
                     FELYELISIQVAGGTHLSPTATVRLSYGDEVIDESAIGDGMIDAACGAIQRATGLEAR
                     LESFTVSSVTGGIDALGDVTIVLAIGDGSPESPTRRATGRGVSTDVVEASARAYLAAV
                     NRLVHLKTREPVVS"
     gene            complement(2264..3265)
                     /gene="ilvC"
                     /locus_tag="VNP73_01095"
     CDS             complement(2264..3265)
                     /gene="ilvC"
                     /locus_tag="VNP73_01095"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWL64545.1"
                     /translation="MAAKIHYDDASLEPLQGKRLTVLGYGSQGRAHALNLKDSGLDVT
                     VGLRKDSDSWTRAEADGLNVAGMNEACSDSDLIAMLVPDQHQASVYSESVEPNLAPGA
                     ALLFAHGFNVHFGQIRAPKDTDVIMIAPKAPGPLVRRTYEEGSGTPGLVAIHQDATGR
                     AMDRALAYGKGIGCTRAGVIETTFAEETETDLFGEQAVLCGGTSRLIQAGFETLVRAG
                     YQPEIAYFECMHELKLIVDLLYEGGFNGMRNAISDTAEYGDLTRGRKVVTEETEQAMQ
                     TILDDIRSGAFAREWVLENKAGTPVLDAERRRQAEHPIEEVGSKLRKMMSWMQRGKS"
     gene            complement(3280..3813)
                     /gene="ilvN"
                     /locus_tag="VNP73_01100"
     CDS             complement(3280..3813)
                     /gene="ilvN"
                     /locus_tag="VNP73_01100"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWL64546.1"
                     /translation="MTTHTLSVLVENKPAVLARISGLFARRGFNIDSLAVGETEDPRV
                     SRMTIVVSVEGKPLEQVTKQLQKLINVLKIAELPHDASVERELALIKVHSEPAKRSEI
                     LGIVEIFRAKITDVDAGAVMIEATGTPDKIASLEQLLAPYGIVEMVRTGRVALARGSG
                     GLKPPALRPVPIESQAS"
     gene            complement(3810..5597)
                     /locus_tag="VNP73_01105"
                     /pseudo
     CDS             complement(3810..5597)
                     /locus_tag="VNP73_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            complement(5637..5966)
                     /locus_tag="VNP73_01110"
     CDS             complement(5637..5966)
                     /locus_tag="VNP73_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64547.1"
                     /translation="MRFEIEMAAGAATKSQEHRGQPNGDFVGTAEWQGPGQVALDMDE
                     EHKAFFERYFHSEHSCIAPAGDHEGMKHQRPADSEQAFNHALWELSAYSYKVRQGDSR
                     RHQRGSN"
     gene            6203..6730
                     /locus_tag="VNP73_01115"
     CDS             6203..6730
                     /locus_tag="VNP73_01115"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWL64548.1"
                     /translation="MKDEYIELESQVKPLREIESMSLYLMSYEGAHEIDAPIDEVWRA
                     TTRLDELESWFAWLKHLRISDETLRTGTRFEFVAVSPLPYKMSVSAVATEVTEHELIQ
                     ADIFGDLVGLASLRLEETDTGTIVTLKWDVELMQTAMRLAARVSKPLLRWGQDWAVKI
                     AVAGARRHLESRSER"
     gene            6741..8447
                     /gene="ilvD"
                     /locus_tag="VNP73_01120"
     CDS             6741..8447
                     /gene="ilvD"
                     /locus_tag="VNP73_01120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWL64549.1"
                     /translation="MTDVYSSPSDPAKRVSASLTDGAARSGARAMLKAVGFTDKDLAQ
                     PLIGVATEWIETMPCNFNQRELAMQAKAGIRSGGCTPMEFNTISVSDGVTMGAEGMRA
                     SLVSREVIADSIELVSRGHHFDGVLSIVGCDKTIPAGVMALCRLDVPGLVLYNGSIAP
                     GRWHGKDVTIQDVFEAIGKHAAGDLSDDDLLSLENVACPGAGACGGQFTANTMATAVQ
                     FMGISPFGLSEVPATEPGKKAAAEDAGRMVARLVKENIKPSTIITKEALENAIASIAA
                     TGGSTNGVLHILAIAYELGIDLSIDDFHAIAERTPVVASLTPGGEYVATDLYKAGGVA
                     LVARELAKKDLIHTDQMTVDGRTIGEIAEAAEEYVGQKVVVPIETPLKPTGGLAILRG
                     NLAPQGCVVKLAGHERNFHRGPARVFDSEEACFEAVEKQSIKAGDVVVIRYEGPAGGP
                     GMREMLHVTAALVGEGLGDQIALITDGRFSGATHGLMVGHISPEAARGGPIAIVRDGD
                     EIEIDVQARELRVKLDQEEIEKRLADWQAPAPRYASGVFAKYAASVASASEGAVTRPA
                     GG"
     gene            complement(8425..8931)
                     /locus_tag="VNP73_01125"
     CDS             complement(8425..8931)
                     /locus_tag="VNP73_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL64550.1"
                     /translation="MTLRPCTESDIGTLHALWSDPDVRRWLWDDRITELKEVQDLVAE
                     SLESFRTRDFGLWMAEREGRVVGFCGLRDATDWLPGQIELLYGLEPKYWGLGYATEAS
                     VALLGHAFDVIGLDQVVAAADSPNEGSVRVLERIGMTLEREGYIENLPTKFFSLKKED
                     FVSPQRGG"
     gene            complement(8948..>9541)
                     /locus_tag="VNP73_01130"
     CDS             complement(8948..>9541)
                     /locus_tag="VNP73_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64551.1"
                     /translation="KVVSTPSVTPAVGCHDGAIAGNLAYMACLSEGQVWDITDPLNPA
                     ILSHINDVPDAIWHSSDTSNDGKIAIFGFESFGPGNTSCNGSGQGAGGALWFYDVSDP
                     TAPAQLGHFVPPRLIEGLCTAHNFAVIPKIERDALVTAWYAGGFMVVDFSDPAAPTEF
                     GWYLPSSTSTWDAKWYRGYAYVGDGSRGLDVYEVSGL"
BASE COUNT         1684 a         3032 c         3027 g         1798 t
ORIGIN      
        1 gtcgggcggg atgatccccg acttggctcc tgcttcgata gccatgttcg tcaaggtcag
       61 ccgggaatcg atcgagagat gttgtagcgc cgatcccgtg tgctccatcg ccatgtaaga
      121 cgcgccgtcg tcaccgatcc gagcgatcac agtgaggatc aggtccttcc cgacgaccca
      181 cgggccgggc cggccttcgt agacgatctt catcgactcg gggacgcgca gccatatctc
      241 gccggtcgcc cataccgcag ctatatcggt cgatccgact ccggtagaga agcaacccag
      301 cgcgccgtac gtgcacgagt gcgagtcggc cccgataacg acgtcgccgg gaagcgtcag
      361 ggcctcttcc ggcaacaaga tgtgctcgat cccgccgcga ccgacatcga agaagtgctc
      421 gatgtcctgc tccagggcaa actcacgcat cacgctcatg agaccggccg acgcgatgtc
      481 cttcgccggc acgaagtgag acgctacaag cgccaccttc gagcgatccc ataccttcga
      541 agcgcccatc ttgcggaacg aatcgacggc cggcggagcc gtgacgtcgt tcgccatcgc
      601 aagatccacc ttgcacatga cgatctcgcc gggcgcgacg cgatccttgc cggcgtgagc
      661 cgcaaggatc ttctcggtga tcgtctgagc cggcatcagg aaacgacggg ttcgcgcgtc
      721 ttcaggtgga cgagccggtt gaccgccgcc aggtaggccc gtgcggacgc ttcgacgaca
      781 tcggttgaga ctccgcgtcc ggtggccctg cgggtcggcg attcgggcga accatcccct
      841 atggcaagca cgatcgtgac gtcgccgaga gcatcgatgc cccccgtcac gctcgagacc
      901 gtgaaggact cgagccgcgc ttccaatccg gtcgctcgct ggatcgcgcc gcaggccgcg
      961 tcgatcatcc cgtccccgat cgcggactcg tcgatgacct cgtcaccgta gctaagacgc
     1021 acggttgcgg tcggtgacag atgcgttccg ccagcaacct ggatcgagat caactcgtag
     1081 agctcgaagt caccggccat ctcgtcgacg acgatcgcct cgaggtcgac gtcggtgatc
     1141 cggatcttgc gatcggccag tgccttgaat cgatcgaagg cacgttgcat gtcctcacga
     1201 gagaggttga acccgagatc ctcgagagcc ttggaaaatg cgtgccggcc cgagtgcttg
     1261 ccgagaacga tcttgctgcc ctcgagcccc acgtcgagcg ggtccatgat ctcgtacgtc
     1321 gttcgctcga ccatgactcc gtgctggtgg atcccggcct cgtgtgcgaa agcattcgca
     1381 cccacgatcg ctttgttcgc cggaaccggg tagccggtca gcatcgagac catccgcgac
     1441 gtgcgagcga tctctttcgt gtggatcgcg gtgtcggggc gatcctcggc cttgcgggtg
     1501 cgcagggcca tcactatctc ttcgagcgag cagttcccgg ctcgctcccc gatcccattg
     1561 atggcgacct cgacttgccg ggcccccacc tcgataccgg cgagtgcatt cgccaccgcg
     1621 aggcccagat cgtcgtgcgt gtgcacgctc cacacgacct cacggtcgac ctcgcccatc
     1681 aggaacctga gcaggtctgc gaactcctgc ggcaacgcgt atccgaccgt gtcgggcagg
     1741 ttcaaggtcg tggcaccggc atcggcagca gccttgaaaa ccttggcgca gaactcgggg
     1801 tcggaccgag tcgcgtcctc gcacgagaat tcgacatcgt cgacgtaacg ccgcgctcgt
     1861 gcgacgccgc cgacggccgc ggcgagcacc tggtcgggtg tcatctggag cttcttggcc
     1921 atgtggatct cggacgtggc gatgaacgtg tggatgcggc cgcgctgcgc cggggcgatc
     1981 gcttccgccg cagtatcgat gtcgatcgcg tcgacacgag cgaggccaca gatgaccggc
     2041 ccccgcacgt tctcggcgat cgcacgcacc gcctccttct cgccttccga cgagatcggg
     2101 aaaccggcct cgatgatgtc gacacccaag cgtgcgagtt gctcggcgat ctcgaccttc
     2161 tccttcaggt tcaaggagat gccgggcgac tgctcgccgt cgcgcagcgt ggtgtcgaag
     2221 atgcgtacta cgccggggtc tccgtctttc cctcctccgc ccatcagctc ttaccccttt
     2281 gcatccacga catcatcttg cgaagcttcg aacccacctc ttcgatcggg tgctcggcct
     2341 gtcgacgacg ctcggcatcg agcaccggcg ttccggcttt gttctcgagc acccactcgc
     2401 gcgcgaacgc gcccgaccgg atgtcgtcga ggatcgtctg catcgcctgc tcggtctctt
     2461 ccgtcacgac cttgcggccg cgggtgagat cgccgtactc ggccgtgtcg gagatcgcgt
     2521 tgcgcatgcc gttgaaccca ccttcataga gaaggtcgac gatgagcttg agctcgtgca
     2581 tgcactcgaa atatgcgatc tcgggctggt atccggcccg aaccaacgtc tcgaaaccgg
     2641 cctggatcag gcgtgaggtt cccccgcaca gaacggcttg ctcaccgaaa aggtcggtct
     2701 cggtctcttc agcgaaggtc gtctcgataa cgcccgcccg cgtgcagccg atccccttac
     2761 cgtaagcaag agctcgatcc atggctcggc cggtcgcgtc ctgatggata gcgaccaacc
     2821 ccggcgtgcc actgccttct tcgtaagtcc tgcgcacgag ggggccgggg gccttgggag
     2881 cgatcatgat cacgtcggtg tccttcggcg ctcggatctg gccgaagtgg acgttgaacc
     2941 cgtgcgcgaa caacagggcg gcgcccggcg caaggttcgg ttcgaccgat tccgaataga
     3001 cgctcgcctg atgctgatca ggaacaagca tcgcgatcag atcggaatcg gagcaggctt
     3061 cgttcatgcc ggcgacgttg agcccgtcgg cttccgctcg cgtccacgaa tcggagtcct
     3121 ttcggagccc cacggtgacg tcgagtcccg aatctttgag gttgagcgcg tgcgcacgcc
     3181 cctgagagcc gtagcccagc accgtcaaac gcttgccctg aaggggctcc agtgaggcgt
     3241 cgtcgtaatg gatctttgct gccatcttcc gtactccctt caggaggctt gagactcgat
     3301 cggaacggga cgaagtgccg gaggcttcag accgcccgag ccgcgcgcga gcgcgacgcg
     3361 cccggtgcga accatctcga cgatcccgta gggggcgagc aactgctcga gcgaggcgat
     3421 cttgtccggc gtcccggtcg cctcgatcat cacggccccg gcatcgacgt cggtgatctt
     3481 cgctctgaag atctcgacga tgccgaggat ctccgaacgc ttcgccggtt cggaatggac
     3541 cttgatcagt gccagctccc gctccaccga tgcgtcgtgc ggaagctcgg cgatcttcaa
     3601 gacgttgatc aacttctgga gctgcttggt cacctgctcg agcggctttc cctcgaccga
     3661 gaccacgatc gtcattcgcg acacgcgcgg gtcttcggtt tcgcccaccg cgagcgaatc
     3721 gatgttgaag ccgcgtcggg cgaacagtcc cgatatacgg gccagcaccg ccggcttgtt
     3781 ctcgacaaga accgaaaggg tgtgagtcgt catgccctcg cctccccggc ttggattccg
     3841 ccgaaggcga agtcggagcc gacctcgtgt tgctcggcct cctcttgtcc cggcccgagg
     3901 atgatgtcgt cattcgacgc acctgcggga accatcggga agcacttctc cgccggatcg
     3961 cagcggaagt cgatgacaca cggtcggtca tcgacaccca tggatttgtc gatcacgttg
     4021 tcgacgtcgt ccatcgattc ggcgcgaagt ccgacggcgc cgtaggcctc ggcgagcttc
     4081 acgtaatcgg ggacgtcggg cggcaggtac acctgggagt agcgctcgtc gtagaagagc
     4141 tcctgccact gacgcaccat cccgaggtgc tcgttgttga ggatggcgat cgtgaatggg
     4201 atcttctcga tcgtcgccgt cgccagttcc tgcgcggtca tctggaagca gccgtcaccg
     4261 tcgatcgcaa cgactcggtc gttgggacgc cccactgcgg cccccagggc cgccggcacg
     4321 gcgaatccca tcgttccgag accacccgaa gagatgaacg agtgcggttt gttgcagcgc
     4381 atgtactgcg acgcccacat ctgatgctgg ccgacgccgg tcacgacgat cgcctcgccc
     4441 ttcaatgcat cgtcgaaacg atcgacgacg aactgaggct tgatgcgccc gtcttcctcc
     4501 tgctcgtaag ccagtgggtg ctctcgttgc atcgagttgc attcgtggat ccaggcagat
     4561 agatcgggcc gtccacccat cgtcgcgacg tcgtgcaaca ccgctttggc caactcaccg
     4621 acgatcggct tcgcgtctcc cacgatcggg acgtccacgg ctctgttctt gccgatctct
     4681 gcgggatcgg cgtcggcgtg gatgatcttt gcgtgcggcg cgaacgtgga cagctgacca
     4741 gtgacccggt catcgaaccg agcgcacaac gtgatcagca ggtcggcgcg ctgcatcgcc
     4801 gtcactgcgg cgtagttccc gtgcatcccg ggcatgccga gacacagagg gtggtcgtcg
     4861 gggaacgcgc cccgcgccat cagagtcgtg acgaccggcg ccccggtcgc ctcggccaat
     4921 gccatcagct cttcggtggc gcgacctttg agcacgcccc cacccacata gagcaccggc
     4981 ctggccgccc gtcggatcag atcggcggca gcacggatct gcctgcgatt cggacgagta
     5041 gttggcctgt aaccgggtag ctcgaccgag tcgggccact cgaaactcgc ttgtgcctgc
     5101 agcacgtctt tcgggatgtc gacgagaacc ggaccgggtc gcccggtgga agcgatgtgg
     5161 aacgcttccg cgatagcggt cgggatctca tcgggatcca tcactttgta ggagtgcttc
     5221 gtgatcggca tcgtgattcc ggtgatgtcg gcttcttgga aagcgtcgtt gccgatcacc
     5281 gggcgcgaga cctggccggt gatggctacg agcgggatcg aatccatgaa cgcgtcggcg
     5341 atgggggtta cgaggttcgt cgcccccggt cccgaagttg ccatgcacac accgggacga
     5401 ccggttgcgt gcgcgtatcc ctcagcggcg tggcctgcac cttgttcgtg ccgcatgagg
     5461 atgtgcctga tcgacgactc gagcagtggg tcataggcgg gaaggatcgt tccgcccggg
     5521 atcccgaaga tgacttcggt ccccgcgttc tcgagcgatc ggatcagaga ctgtgcaccc
     5581 gtgatctttg tcatcggggt ctctttcgcg tttggatccg gatacgcgtc atctagctag
     5641 ttagaccccc tctgatgcct gcgggagtcg ccctgcctga ccttgtacga gtaggccgac
     5701 agctcccaca gcgcgtggtt gaacgcttgc tcggagtcgg cggggcgctg gtgcttcatc
     5761 ccctcgtggt cgcctgcggg tgctatgcag ctgtgctccg aatggaagta gcgctcgaag
     5821 aacgccttgt gctcctcatc catgtccagg gcgacctgcc ccgggccctg ccactccgcc
     5881 gttccgacga agtctccgtt tggttgccct cggtgctctt gcgacttcgt cgccgcacct
     5941 gcggccattt cgatctcgaa cctcatctct tgctcctttc cttgcctacg tctggtttct
     6001 ggcctgcttg ctgctgaaat gggggcacta aaaaaccccc cggtccggag ggttaagcgc
     6061 acgccgtctg cccctagggg cgggcgcgcg ctcaggtaat aagtacgagg acgttgcgga
     6121 agttcgtcgc tctcggggcc atgtcttgcc tggtgccttt catctctgtt cctaacgatg
     6181 aaaccccggc gacgttgctg aggtgaagga tgaatatata gaacttgaat ctcaagtcaa
     6241 gccacttcgg gagatcgagt cgatgtcgct gtacctgatg agctacgagg gggcccacga
     6301 gatcgacgcg cccatcgacg aggtgtggag ggcgacgact cggctcgacg agctcgaatc
     6361 gtggttcgcg tggctcaagc atctacgcat ttcggacgaa acgctcagga ccgggacccg
     6421 gttcgagttc gtcgccgtct cgccgctccc ctacaagatg tcggtctccg ccgtcgcgac
     6481 cgaggtgacg gagcacgagc tgatccaggc ggacatcttc ggcgacctcg tggggctcgc
     6541 ttcgcttcgt ctcgaggaga cggacaccgg aacgatcgtg acgctcaagt gggacgtcga
     6601 gttgatgcag acggcgatgc gattggcggc tcgggtatcg aaaccgctgt tgcgctgggg
     6661 tcaggactgg gcggtgaaga tcgcggtcgc cggagcccgc cggcatttgg agtcgaggag
     6721 cgaaaggtag gttcacgacg atgacggacg tttactcgag cccatccgat cctgcaaagc
     6781 gcgttagcgc ttcgctgacc gatggggcgg cgcgctccgg tgcccgggcg atgctgaagg
     6841 cggtcggctt caccgacaag gacctcgcac agcctttgat cggtgtcgct acagagtgga
     6901 tcgagacgat gccgtgcaat ttcaaccagc gagaactcgc gatgcaagcg aaggccggca
     6961 tccgttccgg cgggtgcact ccgatggagt tcaacacaat ctcggtgtcg gacggcgtga
     7021 cgatgggggc cgagggcatg cgcgcctctc tcgtcagccg cgaggtcatc gccgattcga
     7081 tcgagctcgt gtcacgcggg catcatttcg acggggtgct ctcgatcgtt gggtgcgaca
     7141 agacgatccc cgccggggtg atggctctct gccgcctcga cgttccgggc ctggtcctct
     7201 acaacggatc gatcgcgccg ggtcgctggc acggcaagga cgtgacgatc caagacgttt
     7261 tcgaggcgat cggaaagcac gctgccggcg acctctcgga cgacgatctg ttgtcgctcg
     7321 agaacgttgc ctgtcccggg gcgggcgcgt gcggggggca gttcacagcg aacacgatgg
     7381 cgacggcggt ccagttcatg gggatcagtc cgttcggtct gagcgaggtg cccgcaacgg
     7441 agccggggaa gaaagccgcg gcggaagatg ccgggcggat ggtcgcacgt cttgtcaaag
     7501 agaacatcaa gccttcgaca atcatcacga aggaagcgct cgagaacgcc atcgcttcga
     7561 tcgcagctac gggcggttcc acgaacggtg tgctgcacat cctggccatc gcctacgagc
     7621 tcgggatcga tctatccatc gacgacttcc atgcgatagc cgagcgcacg ccggtggtgg
     7681 caagcctgac cccggggggc gaatacgtgg cgaccgatct gtacaaggcg ggtggtgtgg
     7741 ctctcgtcgc gcgcgagctg gccaagaagg atctgatcca cacggatcag atgacggtcg
     7801 acggccgaac gatcggtgag atcgccgaag cggccgagga gtatgtgggg cagaaggtcg
     7861 tcgttccgat cgagacgcct ttgaagccga ccgggggcct cgcgatcctg cgaggcaacc
     7921 tcgcccctca agggtgcgtg gtcaagctcg cggggcacga gcggaacttc catcgcggtc
     7981 cggcccgagt gttcgactca gaagaggcct gcttcgaggc tgtcgagaag caatcgatca
     8041 aggccggcga cgtagtcgtg atccgttacg aggggcctgc gggggggccg gggatgcggg
     8101 agatgttgca cgtgaccgcc gctctagtgg gtgaggggct cggcgaccag atcgccttga
     8161 tcaccgacgg gcggttctcg ggcgcgacgc acggcctgat ggtcgggcac atctcgccgg
     8221 aggcggcgcg agggggcccg atcgctatcg tgcgcgacgg cgacgagatc gagatagacg
     8281 tgcaagcacg agagctgcgc gtgaagctag atcaggaaga gatcgagaaa cgcctggccg
     8341 actggcaggc gccggcaccg cgctacgcat cgggggtgtt cgccaagtac gcggcgagcg
     8401 ttgcttctgc ttcggagggt gcggttaccc gccccgctgg gggctgacga agtcttcctt
     8461 cttcagggag aagaacttcg tcggcaggtt ctcgatgtat ccctcgcgct cgagtgtcat
     8521 cccgattcgc tcgagcaccc gtacggagcc ctcgttgggt gaatcggcgg cagcaacgac
     8581 ctgatctagt ccgatcacgt cgaacgcatg cccgagcaac gcgacggaag cctccgtcgc
     8641 atatcccagc ccccaatact tgggctcgag tccgtagaga agctcgatct gtcctggcaa
     8701 ccagtccgtc gcgtcgcgca atccgcagaa accgacgacc cggccctctc tttccgccat
     8761 ccacagaccg aagtcacgag tacggaacga ttccaggctc tcggcaacga gatcttgaac
     8821 ctctttcagc tcggtgatgc ggtcgtccca caaccaacgc ctcacgtcgg gatcgctcca
     8881 cagcgcgtga agcgtgccga tatcgctttc ggtgcaggga cggagggtca atcgttccgt
     8941 ctggatccta gagaccgctc acctcgtaga catcgaggcc gcggctcccg tcgccaacat
     9001 aggcgtaacc gcggtaccac ttggcatccc acgtggatgt gctggaaggc aagtaccagc
     9061 caaactcggt cggcgccgcc gggtccgaga agtcgaccac catgaagccg cctgcgtacc
     9121 acgccgtgac gagcgcatct ctctctatct tggggatcac tgcgaagtta tgcgcggtgc
     9181 aaagaccctc gatcaagcgg ggcgggacga agtgtccgag ctgcgcgggc gcggtgggat
     9241 ccgagacgtc gtagaaccac aacgcgccac cggcaccttg gccggatcca ttgcacgatg
     9301 tgtttccggg accgaaggac tcgaacccga agatcgcgat cttcccgtca ttggacgtgt
     9361 cggacgaatg ccagatcgca tcgggcacgt cgttgatgtg cgacaggatg gcggggttca
     9421 aaggatcggt gatgtcccac acctggccct cgctcaagca ggccatgtag gcaagattgc
     9481 cggctatcgc gccgtcatga catccaacgg cgggcgtgac gctcggcgtc gaaacgacct
     9541 t
//
LOCUS       DATMED010000046         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4643780, whole genome shotgun sequence.
ACCESSION   DATMED010000046 DATMED010000000
VERSION     DATMED010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4643780"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /gene="dnaE"
                     /locus_tag="VNP73_01135"
     CDS             <1..2204
                     /gene="dnaE"
                     /locus_tag="VNP73_01135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWL64552.1"
                     /translation="DPVRYDLLFERFMNERREEIPDIDLDVESHRREEVLTYILDTYG
                     AEQTAMCCMVDTFRARGAIREVGKALGMPQEEIDVVAKAFPRIAARDIPQAIERLPEL
                     AGTNLRAGQLEDLFGLCQEIDGFPRHLALHPSGVILSSPDLPDRVPMQESFQGFTMLQ
                     ADKDDVEELGLAKLDVLGVRMLSSISHAVSEIKRVHGETIDLSSLPHDNGAAFDLIRS
                     SKTLGCFQIESPGQRELLARFQPSRFEDLIIDISLFRPGPVKSDMIKPFLNRRHGFDE
                     ESYSHECLKPILAETNGVIVYHEQVIRVIAAITGCSLADADYVRRHLDAEQPEKDVGE
                     WFVSSAIRNGFAPAHANALWKEVFAFASFGFCKSHAAAFALPTYLSAWLKAHHPAAFL
                     AGVLTHDPGMYPRRLIVADARHFGIPILPVDVNESHADYRAEQVNEKLGIRLSLSDVR
                     DISEAEVKSLLEARETGPFTSIEDLWRRTELSRPVLENLVHVGALDSIEHNRIRRELL
                     WRILDLVAEPKIRPGVQLAFSLEESVVESLPGLPGYSELEETEAELEITGIDARRHVM
                     SLYEPVMAELGCTPGNMLKAKRNDSEVWVAGVKVASQTPAIRSGQRIIFVTLDDLTGP
                     IDVTIFERVQPLCARTVFHSWLQVVRGVVRKRGGASRRHETDPDNVGITVVAEEVFDL
                     AELAEDMNNGFSVRDALLRQRRKQRAAGLAAAARRTPGGKLWHSSGGSAGG"
     gene            complement(2208..>2526)
                     /locus_tag="VNP73_01140"
     CDS             complement(2208..>2526)
                     /locus_tag="VNP73_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64553.1"
                     /translation="MGAVEPQRTGEVLGAGSVLVGMQLVAFDEAILLAIATGVMVGIA
                     SVGVARDRTAALAVGAVGLVVLVPQLITEVFGPSLGAMGALAISGLLVIAVAVFIARK
                     RRD"
BASE COUNT          477 a          813 c          801 g          435 t
ORIGIN      
        1 tcgatcccgt tcgttacgac cttctcttcg agcgcttcat gaacgaacgt cgcgaagaga
       61 tccccgacat cgacctcgat gtcgaatcgc atcgcagaga agaggtcctc acttacatcc
      121 tcgacacgta cggggccgag cagacggcga tgtgctgcat ggtcgacacg ttccgagcgc
      181 gcggagcgat ccgggaggtc ggcaaggcgc tggggatgcc ccaggaggag atcgacgtcg
      241 tggccaaggc gttcccacgc atcgctgcgc gcgatatccc gcaagcgatc gagcgcctac
      301 ccgagctcgc cgggacgaac ctgagagccg gtcagctcga agatctgttt ggtctctgtc
      361 aggagatcga cggtttcccc cggcatctcg cgctgcaccc ctccggggtg atcctgtctt
      421 ctccggacct ccccgaccgc gtcccgatgc aggagagctt ccagggcttc acgatgttgc
      481 aagccgacaa agatgacgtc gaagagctcg gcctcgcgaa gctcgacgtc ctcggcgtac
      541 ggatgttgtc gtcgatctca catgcggtga gcgagatcaa gcgggtgcac ggcgagacca
      601 tcgacctgtc gtcgttgccg cacgacaacg gtgctgcgtt cgatctgatc cgttcgtcga
      661 agactctcgg ttgtttccag atcgagtcgc cgggccagcg cgagctactg gcacgcttcc
      721 aaccgagccg gttcgaggac ctgatcatcg acatctcgct gttccggccg ggcccggtga
      781 aatccgacat gatcaagccg tttctcaacc gcaggcacgg cttcgacgaa gagagttact
      841 cgcacgaatg cctgaaaccg atcctggccg agacgaacgg cgtcatcgtt taccacgagc
      901 aggtgatccg agtgatcgca gcgatcaccg gttgttcgct cgccgatgcc gactacgtcc
      961 gtcgccatct cgacgccgaa cagcccgaga aggacgtcgg cgagtggttc gtgtcgtcgg
     1021 cgatccgcaa cgggttcgcc cccgcccacg cgaacgctct atggaaggaa gtgttcgcgt
     1081 tcgcgtcgtt cgggttttgc aaatcgcatg cggccgcgtt cgccctccca acgtacctgt
     1141 cggcgtggct gaaggcgcat catccggccg cgttcctcgc cggggtcctg actcacgacc
     1201 cgggcatgta cccacgcagg ctgatcgtgg ccgacgcgcg ccatttcggc atcccgatac
     1261 ttccggtgga cgtcaacgaa tcccacgccg actaccgcgc tgagcaagta aacgaaaaac
     1321 tcggcatccg tctgtcgctg tccgatgtgc gggatatctc cgaggccgag gtgaagtcgc
     1381 tgctcgaggc gagggagacc gggccgttca cgtcgataga agacctgtgg cgccggaccg
     1441 agctgtcccg gccggtgctg gagaacctcg tgcacgtcgg ggcattggac tcgatcgagc
     1501 acaaccggat ccggcgcgag ctgctgtggc ggatcctcga tctggtcgcg gagccgaaga
     1561 tccggcccgg cgtccagctg gcgttctcgc tggaggaatc ggtcgtggag tcgctgccgg
     1621 gccttcccgg ctacagcgaa ctggaggaga ccgaggccga gctcgagatc accggcatcg
     1681 acgctcggag acacgtcatg agcctctacg agccggttat ggccgagctc gggtgcacgc
     1741 ccggcaatat gttgaaggcc aaacgcaacg actccgaggt gtgggtggca ggagtcaagg
     1801 tagcgagcca gacgcccgcc atccgatcgg ggcagcggat catcttcgtc acgctcgacg
     1861 atctcaccgg gccgatcgac gtcacgatct tcgagcgcgt ccaacctctt tgtgcgcgca
     1921 cggttttcca ttcctggctc caggtcgtcc gcggcgtggt gagaaagcgc gggggggcgt
     1981 cgcgccggca cgagaccgat cccgacaacg tggggatcac ggtagtggcg gaagaagtct
     2041 tcgacctggc cgagctggcg gaggacatga acaacggctt ctcggttaga gacgcgctac
     2101 tgcgccaacg ccggaagcag agagcagccg gtctggccgc agcggctcgc cgcacccccg
     2161 ggggaaagct atggcactcg agcggcggca gcgcgggtgg ttgagcctca gtccctgcgc
     2221 ttgcgtgcga tgaagacggc aaccgcgatg acgagcaagc cggatatcgc gagtgctccc
     2281 atagcgccca gacttggtcc gaacacctct gttatcaatt gcggcacgag cacaaccagc
     2341 ccaacggctc cgaccgcgag tgcagcagtg cggtctcggg caacaccaac ggaggcgatc
     2401 ccgaccatca cgccggtcgc tatggcaaga aggatcgcct cgtcgaacgc cacgagttgc
     2461 atcccgacca gaacggatcc agctccaagg acttcaccgg tccgctgcgg ctcaacagca
     2521 cccatc
//
LOCUS       DATMED010000047         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3109572, whole genome shotgun sequence.
ACCESSION   DATMED010000047 DATMED010000000
VERSION     DATMED010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109572"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VNP73_01145"
     CDS             complement(<1..233)
                     /locus_tag="VNP73_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWL64554.1"
                     /translation="MQIATRVVSAGGHSSRVLEGGSGPPVVVLHGWGGRIESMQPVID
                     CLSTGARVVAIDLPGFGDSPPPAGVWGTPDYAA"
     gene            314..976
                     /locus_tag="VNP73_01150"
     CDS             314..976
                     /locus_tag="VNP73_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64555.1"
                     /translation="MKKQIVLVLALLLLSAPHAQGQSVGVGGQEVVEARASWTLRNDQ
                     TQAVFRFVNVYRTYDPIGSTDTFAALGKTRCRLDEDGNTYLSTCTVTMRIIFFKPQYF
                     SFDPFLGGASVVLKDGTRTHVIKWEPKSDRPSPDYVLQGGERSLVFYGLLRSQADAKG
                     RVLGERFSTLEPGGHAALTRGVEATLLWDTGEERSTRTYRHRSGTRAEAWRWIGSLSR
                     GI"
     gene            complement(977..1114)
                     /locus_tag="VNP73_01155"
     CDS             complement(977..1114)
                     /locus_tag="VNP73_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64556.1"
                     /translation="MKDKDRPDPAPDPPEDTEHPSEEKDDSDVDDESGDSFPASDAPA
                     W"
     gene            complement(1155..2291)
                     /locus_tag="VNP73_01160"
     CDS             complement(1155..2291)
                     /locus_tag="VNP73_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64557.1"
                     /translation="MKRLVTAILTLGLMVGGSVAADADAFDKTDNVEVITSIPYSGGS
                     DIAFERGRVYFGQLGNEGGVRILDTSKRVPKEMAFIQCPGTQNDVAVVRPGLLALGYH
                     SGQCGGIPGAGVRLIDVSNPKRPRFLGSVMLPGGSHTITVYPGSDIIYASPGGLANGG
                     GVEQILDVSNPKKIEVAATFKPNPTGCHDITFGKAADGDRMLGFCAGGGEVSIWDVTD
                     PLAPVVIAHGFTPSFFPHAAVPTPDGEYLVMTDEAFGVHDCVGGPTGSVWVFDITTPE
                     VPLLVGHWGPQRGASPVGGAAGAWCTAHNLNFVGTTRQAVISWYSGGTSVVDFSNPTL
                     PQEIAYFRADDSDSWSSYWFDGQIYINDLNRGIDVIEVKGLKAK"
     gene            2432..2791
                     /locus_tag="VNP73_01165"
     CDS             2432..2791
                     /locus_tag="VNP73_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL64558.1"
                     /translation="MRVLAVEDDPEFREFLSFHLSSQGGIEIEVAATGEEALAALSER
                     APDVLVVDVNMSEMGGEELARRARERHPAIKVVSFSGSGGDTPWADQTIVKGGRESLT
                     QLVEAIRALAEEPARDR"
     gene            2864..3706
                     /locus_tag="VNP73_01170"
     CDS             2864..3706
                     /locus_tag="VNP73_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64559.1"
                     /translation="MFDDLDSYWGDLEPSLRKRFLLFTPWDVSWDDPVVTWDPEDGVE
                     EFLDLAQTLGVPMLYVTVSRFNEETLSDLQRRLLDPAGPHPETGSILTRAEQYVDHIS
                     SIEIGWMYQGVGHFCEIEASWYRHLEDEIAEEQEFDEMQHAAERMLFEEQLSLVPEWA
                     ERVAQLPQFQQARTMLQRADLVPRLLPEIEEVTAQYRHGSWIARRLARDVAREAFRIY
                     VYEVLPAQEIELASEAHRLLTTGMRKYEVAARLGISKERLNRLIVRYPPQPDEPSAPS
                     VGDA"
     gene            3747..>4207
                     /locus_tag="VNP73_01175"
     CDS             3747..>4207
                     /locus_tag="VNP73_01175"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HWL64560.1"
                     /translation="MIGSLINVAAIVVGTTIGLLLGNRLPDRIRRTVTDGIGLFVVVL
                     GVANALETFGAALSEALGRAAVVVILGSLIVGAVLGELLDLDGKLTRIGERLRDRFAS
                     GNGSHFVEGFVAMSLIACVGPLTLLGALQDGLSGDYELLSVKSMLDGFTSLA"
BASE COUNT          770 a         1324 c         1334 g          779 t
ORIGIN      
        1 aaggcggcat agtccggcgt gccccacaca cctgcagggg ggggcgagtc cccgaaaccg
       61 ggaagatcga tcgccacgac ccgagctccc gtcgaaaggc agtcgatcac gggttgcatc
      121 gattcgatac ggccccccca cccgtgaagc acgaccacgg ggggcccgct gcccccctcc
      181 aggacgcggc tcgagtgacc gcctgccgac acgactctgg ttgctatctg cacgcatcga
      241 agtatgcctg cgggggtagg acttgagtcc aaacgggcga atcttttagg taagcagcgg
      301 aggtttcggt agcttgaaga agcagatcgt cttggttctc gcccttctcc ttctaagcgc
      361 gccgcacgcg cagggtcaat ccgtcggggt cgggggccag gaagtcgtag aggcccgggc
      421 gtcgtggacg ctccgcaacg accagacaca agcggttttt cgcttcgtga acgtctatcg
      481 cacgtacgac ccgatcggtt ccacggatac cttcgcagcg ctgggcaaaa cgcggtgccg
      541 gctcgatgag gacgggaaca cctacctgtc cacctgcacg gtcacgatgc ggatcatctt
      601 cttcaagccg cagtacttct cgttcgatcc cttcctcggt ggcgccagtg tggtgctcaa
      661 agatggcacc aggacacacg tgatcaagtg ggagccgaag tccgaccgcc cctcgccgga
      721 ctacgtactc cagggcggcg agcgcagcct cgtcttctat ggacttcttc gatcgcaagc
      781 cgacgcgaag ggcagggtgt tgggcgaacg cttctcgacc ctcgagcccg gcgggcacgc
      841 cgctctgaca cgtggggtcg aggcgacact tctctgggat accggcgagg agcgctcgac
      901 gaggacatat cggcaccgat ccggaacgcg cgcagaagcg tggagatgga tcggatcgct
      961 ctcgcgaggg atctagctac caggccggcg cgtcgctcgc cggaaacgaa tccccagact
     1021 catcgtcgac atccgaatcg tctttctctt cgctcggatg ctccgtgtcc tcggggggat
     1081 cgggtgcggg atccggccgg tccttgtcct tcataggact atcttctccg gaccgtcaac
     1141 atcccaaccg ccggctattt agcctttagg cccttcacct cgatcacatc gatgcctcgg
     1201 ttgagatcgt tgatgtagat ctggccgtcg aaccagtagg acgaccacga gtcggaatcg
     1261 tcggcccgga agtacgcgat ctcttgcgga agcgtcgggt tcgagaagtc gacgacactg
     1321 gtgccgcccg agtaccagga gatgacggcc tgccgggtcg tgcccacgaa gttcaggttg
     1381 tgagccgtgc accaggcgcc ggcggcgccc ccgacggggg atgcacctcg ttgtggaccc
     1441 caatgtccga cgaggagagg aacctcagga gtcgtgatgt caaagaccca cacggatccg
     1501 gtcggacccc ccacgcaatc gtggaccccg aaagcctcgt cggtcatcac gaggtattca
     1561 ccatccggcg tcggaacggc ggcatgggga aagaacgacg gggtgaatcc gtgagcgatc
     1621 acgaccggcg caagcggatc cgttacgtcc cagatcgaga cctcgccacc gccggcgcag
     1681 aaaccgagca tccggtctcc gtcggccgct ttcccgaacg tgatgtcgtg gcacccggtt
     1741 gggttgggct tgaaggtcgc cgcgacctcg atctttttgg gattggacac gtcgaggatc
     1801 tgttccacgc cgccaccgtt tgcgagccca ccgggcgacg cgtagatgat gtcgctaccg
     1861 gggtagacgg tgatcgtgtg gctccctccg gggagcatca cgcttccgag aaagcgaggc
     1921 cgcttggggt tggacacatc gatcaggcgc acgccggcac cggggatgcc tccgcactga
     1981 ccgctgtgat agccgagcgc cagaagcccc ggcctcacca cggcgacgtc gttttgggtg
     2041 ccggggcatt ggatgaaggc catttccttt gggacccgct tggaggtgtc gaggatccgg
     2101 acgccgccct cgttgccgag ctgtccgaaa tagacgcggc cccgttcgaa ggcgatgtcg
     2161 cttccaccgg aataagggat cgaggtgatg acctcgacgt tgtccgtctt gtcgaaggcg
     2221 tcggcatcgg cggctaccga gccccccacc atcagcccca gcgtcaggat cgcggttacg
     2281 agtcttttca cgtcagtccc cttctcgata tctgtcagaa gggttctcct cggccccccc
     2341 gcctcctctg gggtggctcc ggcagcgtaa attggcaaga caagccaatt tgaggtattt
     2401 agccccgttc gatcacccac gggaggcatg agtgagggtt ctggccgtcg aagacgaccc
     2461 cgagttcagg gagttcttga gcttccatct gtcgagccag ggcggcatcg agatcgaggt
     2521 tgccgccaca ggagaggagg ccctggcggc cctctccgag cgggcccccg acgtcctcgt
     2581 ggtcgacgtg aacatgtccg agatgggtgg agaggaactc gcccgacggg cgcgggagcg
     2641 acatccggcc atcaaggtcg tttcgttctc gggaagcggc ggggacaccc cgtgggccga
     2701 ccagacgatc gtcaagggag ggcgggagag cctcactcag ctggtcgagg cgatccgggc
     2761 ccttgccgag gaaccggccc gggaccgtta gatctacgta cttatcccgc ggcccccggc
     2821 tgccgaataa ataagaggca gaccctgttt caattgagaa gagatgttcg acgacctaga
     2881 ttcctattgg ggcgacctgg aaccctcctt gcgaaagagg ttcctcctct tcacgccatg
     2941 ggatgtcagt tgggatgatc ccgtcgtcac gtgggatccc gaagacggcg tcgaagaatt
     3001 cctggacctt gcgcagacac tcggcgtccc gatgctttac gtcactgtgt cgcgcttcaa
     3061 cgaagagacg ctcagcgatc tacaacgaag acttctcgac cccgcggggc cccatcccga
     3121 gaccggctcg atccttaccc gagccgagca gtacgtcgat cacatctcct ccatcgagat
     3181 cggttggatg taccagggcg tcggccactt ctgcgagata gaggcaagtt ggtaccggca
     3241 cctcgaagac gagatcgccg aagagcagga gttcgacgag atgcaacacg ccgccgaacg
     3301 catgctgttc gaggagcagt tgagcctcgt acccgaatgg gccgagcgcg ttgctcagct
     3361 accgcagttc caacaggcac gcacgatgct tcaacgcgcg gacctcgttc ctcgcctcct
     3421 tccggagatc gaggaagtaa ccgcccagta ccgtcacggc tcgtggatcg ctcgtcgcct
     3481 cgctcgtgac gtcgcgcgtg aggcgttccg catctacgtc tacgaggtat tgcccgcgca
     3541 ggagatcgag ctcgctagcg aggctcaccg tctgcttacg accgggatgc gcaagtacga
     3601 ggtggcggct cgactgggta tctccaaaga gcgactgaac cggctcatcg tgcgttaccc
     3661 gccgcagccg gacgaaccct cagccccttc ggtgggagac gcctagccct tccgcaccgc
     3721 atcgaccggg tgattagggt ccccggatga tcggatcctt gatcaacgtt gccgcgatcg
     3781 tcgtcgggac gacgatcggt ctcttgctcg gcaaccgcct accggaccgg atccgacgaa
     3841 ccgtcaccga tgggatcggg cttttcgtcg tcgtgctcgg tgtagcgaac gcactggaga
     3901 cgttcggagc agccttgtcc gaagcgttgg gtcgcgctgc ggtcgtcgtg atcctcggct
     3961 ctctgatcgt gggagcggtg ctgggagagt tgctcgacct agacggcaag ctgacccgga
     4021 tcggcgagcg cttgcgcgac cggttcgcga gtggcaatgg atcccacttc gtcgagggct
     4081 tcgtggcgat gagcctcatc gcgtgtgtgg gcccgctgac gctcctcggc gcactccagg
     4141 atggtctgtc cggggactac gaactcctgt ccgtgaagag catgctcgac gggttcacct
     4201 cgctggc
//
LOCUS       DATMED010000048         5196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_419001, whole genome shotgun sequence.
ACCESSION   DATMED010000048 DATMED010000000
VERSION     DATMED010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419001"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VNP73_01180"
     CDS             complement(<1..877)
                     /locus_tag="VNP73_01180"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWL64561.1"
                     /translation="MKAFITGATGFVGGKLAAKLRDRGDDVVALVRSPAKAARLKELG
                     CTIVEGDLLAAAAIREGVRGADAVFHVAADYRIGIPASERKAMHEANVTGTEVVLDAA
                     SEAGVTKIVYVSTIGYFGNTRGEVVDEKFQRTDLDWLSAYDETKYLAHELVQDRIDQG
                     APIVITQPGGIYGPGDQSDLADLIDRVRKGKLPFLPMPEVGFNFVHVDDVVEGLLLAH
                     DKGKPGESYVLGGELTTLGGFVETLTGLEGRRPPRVLPTVFVKVSAPFGPIVGKAFGL
                     PPNMREMIKAGDGVTY"
     gene            933..2192
                     /locus_tag="VNP73_01185"
     CDS             933..2192
                     /locus_tag="VNP73_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL64562.1"
                     /translation="MRAPATFESLKIRNYRIFFAGQVLNWTGTWVSWVAQGWLVLRLT
                     DSGFSLGLITALQWSPILLFGAWAGVVADRFEKRKILLFTNGSAAILSLILGIATVAG
                     WVTLPLVIVVAVLLGLVTALDNPTRQAFTMEMVGRERITNAVSLNTATFTIARVVGPV
                     IAALLIKTVGIGECFLVNTVSFIPISVALLRMDRSQLMPVPAVERGPRQVREGMRYVG
                     SLPVLKVLLIMMAVIGTFQYNFQVILPLLAKHTFHGDAGTFGLLGATLGAGMFIGSMT
                     NATFGRPGRRIILVAGFSLALFTFAVAAAPTLLLAALMMIPLGASSMAFLATMNSSLQ
                     LTSSDAMRGRVMALYFVLFLGSTPIGAPILGWVAEAISPRAAFAFGATATLVACAYGY
                     FKLPAIKTEPRGSTVPDELEEALQEAR"
     gene            complement(2193..2936)
                     /locus_tag="VNP73_01190"
     CDS             complement(2193..2936)
                     /locus_tag="VNP73_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWL64563.1"
                     /translation="MSDDLTIEELARATGLTVRNIRAYQTKGLLSPPGLRGRTGIYGS
                     AHLGRLRVVKEMQANGFSLRSIKSLLDAAPEGAEEELLRFEKALMAPWTSEEPEILTA
                     AEIAARFGSPSAEVGRRAAALGVLRPLPDGNFEVRIPTLLAAAQELHAMGVTLERMLD
                     VLERVEHNSKQVTKIFVDLFLEHLWRPFDQAGRPPERWKEVRESLEQIRPIASRVVNA
                     AFAAAMEHAVEEAFSEELESHSQLAQEIG"
     gene            3002..4813
                     /locus_tag="VNP73_01195"
     CDS             3002..4813
                     /locus_tag="VNP73_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64564.1"
                     /translation="MVAYLWAQSGAPAGGAPTEDLIPAGVVGGLAILGLLVLAEIYRR
                     GGARPLRRLADWAGSISSLPGWAALPAGVAAGSLIGAVFGFYWDVSTHIDNGRDPGPF
                     ANPSHYFILFGLAGIALAGYLSLLLASDSKRERTFIGPAPLGGALLALCGVIALSGFP
                     LDDVWHRLFGQDVTLWGPTHIQMVGGAALTTLATWVLVREGQRAQGATDALTKPQRVQ
                     EFMLAGAFMVGLSALQAEFDFGAPQFQLLYQPVLLMVAAGIGLVAARVRLGRGGALGA
                     VLFFLALRGLLALIVGPVLGRTTPHFPLYLVEALVVEAVALWISPRRTFAFAAAAGVG
                     IGTLGLAAEWTWSHIWLPIAWPSDLLPEGLLLGFLAAFAAAIVGGFIGGTLNSDGSRV
                     SVIRPAPALAALVVVILCIAFPAPTSPPSNVGAEVDLAYVERDGVRMANVTVRPEPDS
                     ITESPMWFELLSWQGRDWNRGSKIVAALEETEPSTFKSSQPVPIEGDWKTFLRLHNEG
                     YLVALPIYMPKDSAIPAPEIPATASFQRDFVPDKEILQREATTDGGALQRVAYGILGA
                     IALAWIAAFAWSLSRFGARPQKRTGQRVRSTVAEASG"
     gene            complement(4810..>5196)
                     /locus_tag="VNP73_01200"
     CDS             complement(4810..>5196)
                     /locus_tag="VNP73_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWL64565.1"
                     /translation="WAHTSSQRARLTGANVAPGPGDENGSGRALIKAAPEAATVCYKI
                     YFSGIGRAKSAHIHEAPKGETGPKKFLLFSSTKGRSSPVEGCRHNLDPAEIEEMHEAP
                     ARHYVDVHTGRHPAGALRGQLRPADD"
BASE COUNT          825 a         1662 c         1663 g         1046 t
ORIGIN      
        1 aataagtgac cccgtctccg gctttgatca tctccctcat attgggagga aggccgaatg
       61 ccttcccgac gatcggcccg aagggggcgg aaaccttcac gaaaacggtc ggcagcacac
      121 gcgggggcct gcgcccctcg aggccggtca gcgtttcgac gaagccaccc agggtggtca
      181 actccccgcc gagcacatag gactcacccg gcttgccctt gtcgtgagcc agaaggagcc
      241 cttcgaccac gtcgtccacg tgaacgaagt tgaaaccgac ctcaggcatc ggaaggaacg
      301 gcagcttgcc tttgcgcacc cggtcgatca ggtcggcgag gtcggactgg tcccccggcc
      361 cgtagatccc gcccggctgg gtgatgacga tcggagcgcc ctggtcgatc cggtcctgga
      421 ccagctcgtg ggccaggtac ttggtctcgt cgtaggccga gagccagtcg aggtccgtcc
      481 tctggaactt ctcgtccaca acctcgcccc gcgtgttgcc gaagtagccg atcgtggaca
      541 cgtagacgat cttggtgaca ccggcctcgc ttgcggcgtc gagcaccacc tccgttccgg
      601 tgacgttcgc ctcgtgcatc gccttgcgtt ccgacgccgg gatgccgatg cgatagtcgg
      661 ccgcaacgtg gaagaccgcg tcggcacctc gaacgccctc tctgatcgcg gcggcggcca
      721 gaagatcgcc ctcgacgatc gtgcaaccga gctccttgag gcgtgctgcc ttggccgggc
      781 tgcgcacaag cgcgacgaca tcgtctcctc ggtcgcgaag tttcgcggcg agtttcccgc
      841 cgacgaatcc ggtagccccg gtgatgaatg ctttcatccg atcccccttg cccgggcgcg
      901 agcgtaaacc gtcgtccaat aacctcggtg cgatgagagc ccccgcaacc ttcgagtcgt
      961 tgaagatccg caactacagg attttcttcg ccgggcaggt cctgaactgg acgggtacct
     1021 gggtctcatg ggtcgcgcag ggctggctgg tgctgagact gacggacagc ggcttcagtt
     1081 tggggctcat caccgcgttg cagtggtcgc cgatcctgtt gttcggtgcg tgggccggag
     1141 tcgtcgcgga tcggttcgag aagcgcaaga tcctgctctt cacgaacggc agcgcggcga
     1201 tcctctcgtt gatcctaggg atcgcaacgg tcgcggggtg ggtgaccctt ccgttagtga
     1261 tcgtcgtcgc ggtgcttctc ggactcgtga ccgcgctcga caacccaacg cgccaggcct
     1321 tcacgatgga gatggtgggg cgcgagcgga tcacgaacgc cgtaagtctg aacacagcga
     1381 ctttcacgat cgctcgtgtc gtcggtcccg tgatcgccgc tctgttgatc aagaccgtgg
     1441 ggatcggcga gtgcttccta gtgaacacgg tctcgttcat cccgatctcc gtggcgctgt
     1501 tgaggatgga ccggtcgcag ctgatgccgg tgccggcagt cgagaggggg ccgaggcagg
     1561 tccgggaggg gatgcgctac gtgggttccc tgccggttct gaaggtgctc ttgatcatga
     1621 tggccgtgat cgggaccttc cagtacaact tccaggtcat ccttcctctt cttgcgaagc
     1681 acaccttcca cggcgatgcg ggaaccttcg gtcttctagg agcgaccctc ggggccggga
     1741 tgttcatcgg gtcgatgacg aacgcgactt ttgggagacc cggtcgccgg atcatcttgg
     1801 ttgcaggctt ctctctcgcg ctattcacgt tcgccgtggc cgccgctccc acactgctac
     1861 tggcggcatt aatgatgatc ccgctgggcg cgtcatcgat ggccttcttg gcgacgatga
     1921 actcgagcct tcagctcaca tcgtccgacg cgatgcgagg gcgcgtcatg gcgctctact
     1981 tcgtcctgtt cctcggatcc acaccgatcg gtgcacccat cctgggatgg gtcgcggaag
     2041 cgatcagccc gcgcgcagcg ttcgcgttcg gggcaaccgc gaccttggtc gcgtgcgcgt
     2101 acggctattt caaactgccg gcgatcaaga ccgaacctcg aggatcgacg gttccagacg
     2161 agttagagga agcgttgcaa gaagcgcgct gatcaaccga tctcttgcgc gagttgcgaa
     2221 tgcgactcga gctcttccga gaacgcttcc tcaactgcat gctccatagc ggcggcgaac
     2281 gcggcgttca ccactcgcga cgcgatcgga cggatctgct cgagcgactc ccggacctct
     2341 ttccatctct cgggtggtcg gccggcctga tcgaacggac gccacagatg ctccaggaaa
     2401 agatcgacga atatcttggt cacctgtttc gagttgtgct cgacgcgctc gagcacgtcg
     2461 agcatccgtt cgagggtcac tcccatcgcg tgcagttcct gagccgcggc cagaagcgtc
     2521 ggtatgcgga cctcgaagtt cccgtcgggc agcggacgaa ggacaccgag cgcagccgca
     2581 cgacgcccaa cctctgcaga aggggatccg aagcgggctg cgatctccgc cgcggtgagg
     2641 atctccggtt cttcggatgt ccacggcgcc atcaacgctt tctcgaagcg aaggagctct
     2701 tcctcggccc cctcgggagc cgcatccaat agagacttga tcgatcgcaa gctgaagccg
     2761 ttcgcctgca tctccttgac cacgcgtagg cggccgaggt gggccgaacc atagatgccg
     2821 gtgcgacccc gcaggccggg tggggacagg agccccttgg tctggtacgc ccggatgttg
     2881 cggacggtta ggcccgtggc tcgcgccagc tcctcgatcg tcagatcatc gctcacgagg
     2941 tttattttac accaaatagt gttacattca ttgatgtaat cattctcatg gaggtggcgg
     3001 catggtggct tatctatggg cacagtccgg agctcccgcg gggggcgccc ccactgaaga
     3061 tctcatccct gcaggggtcg ttggtgggct cgcgatcctg ggccttctcg tccttgccga
     3121 gatctacagg cgcggcggtg cccgacccct acgtcggctc gccgactggg caggatcgat
     3181 ctcatctctg ccgggatggg cggcgctgcc ggcaggggtc gctgccggct cattgatagg
     3241 cgctgtgttc ggtttctact gggatgtctc gacccacatc gacaacggca gggatcccgg
     3301 ccccttcgcg aacccgtccc actacttcat cctgttcggc ctcgccggga tcgccctcgc
     3361 gggctatcta tcgcttctcc tcgcgagcga ttcgaagcgc gagcgaacct tcatcggtcc
     3421 cgctcctctc ggcggcgccc ttctcgcgtt gtgtggagtc atcgcgctga gtgggttccc
     3481 gctcgacgac gtgtggcacc gcttgttcgg tcaggacgta acgctgtggg gaccgacgca
     3541 tatccagatg gtcggcggcg cggcattgac gacgctcgcg acatgggtgc tcgtcaggga
     3601 gggccagcgc gcccaaggcg caaccgacgc gctcacgaag ccacaacgtg tacaggagtt
     3661 catgctcgcc ggcgccttca tggtggggct ttctgcgctg caggccgagt tcgatttcgg
     3721 cgctcctcag ttccagctgc tgtatcagcc cgtgctgttg atggtcgccg ccggcatcgg
     3781 cctcgtcgcg gctcgagtga ggctgggtcg cggaggagcc ttgggggccg ttctcttctt
     3841 cctcgcgctg cgtgggttac tggcactgat agtgggaccg gtgctaggcc gtaccactcc
     3901 tcacttcccc ctctatctag tcgaggctct agtcgtcgag gccgtggcat tgtggatctc
     3961 gccgcgtcgc acgttcgcgt tcgcggcggc ggctggggtt gggatcggaa cgctcggcct
     4021 cgccgccgag tggacgtgga gccacatctg gctcccgatc gcgtggccga gcgaccttct
     4081 gccggagggc ttgttattgg gattcctggc cgcgttcgcc gctgcgatcg tcggtggctt
     4141 catcggtgga acgctgaact cggacgggtc ccgggtctcg gtcatacggc cggcccccgc
     4201 tcttgccgcc ctggtcgttg tgatcctgtg catcgcgttt cccgccccca cttcgcctcc
     4261 atccaacgtg ggggccgagg tggatctggc ctacgtcgag cgtgacggag ttcggatggc
     4321 gaacgtcacc gtgcgaccag agcccgactc gatcaccgag tcgccgatgt ggttcgagct
     4381 tctctcctgg cagggccgtg actggaaccg cggctcgaag atcgtcgcgg ctctcgaaga
     4441 gaccgagccg agcacgttca agtccagcca acccgttccc atcgagggtg actggaagac
     4501 cttcctgaga ttgcataacg agggttatct cgtcgcgctg cccatctaca tgcccaagga
     4561 ttccgccatc cctgcaccgg agataccggc gaccgcgagc ttccagcgcg acttcgtccc
     4621 cgacaaggag atccttcagc gggaggcgac aacagacggt ggcgctctcc agagggtcgc
     4681 ttacgggatc ctcggtgcga tcgctcttgc gtggatagca gcgttcgcct ggagtctgtc
     4741 gcggttcgga gcccgtccgc agaaacgcac gggacaacgc gtcaggagca cggtcgcgga
     4801 agcttccggc tagtcgtcgg cgggcctaag ttgcccgcgt aacgcaccgg ccgggtgtct
     4861 accggtgtgg acgtcgacgt aatggcgagc aggggcctcg tgcatctcct cgatctcggc
     4921 cggatcgagg ttatgccggc atccctcgac cgggctgctc cggcccttgg tggacgagaa
     4981 cagcaggaac ttcttcggac ccgtctcccc cttcggggcc tcgtggatgt gcgccgactt
     5041 ggctcgaccg atcccggaga agtagatctt gtagcaaacc gtggccgctt ccggagcggc
     5101 tttgatgagc gcgcggccgg acccgttttc gtctccgggt cctggagcga cgttggctcc
     5161 cgtcaaacgg gcacgctgac tcgaggtgtg ggccca
//
LOCUS       DATMED010000049         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_420540, whole genome shotgun sequence.
ACCESSION   DATMED010000049 DATMED010000000
VERSION     DATMED010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420540"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..2021
                     /locus_tag="VNP73_01205"
     CDS             168..2021
                     /locus_tag="VNP73_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWL64566.1"
                     /translation="MAQAPPAADRRELERVVIRFAGDSGDGMQLTGDRFTSVSAVFGN
                     DLATFPDYPAEIRAPAGTLAGVSAFQIHFADYDILTPGDQPSVLVAMNPAALKANLKD
                     LISGGLLIVNTDAFDEKNLDKVGYPSNPLKDGSLGDYQVYEIPMTSMTIEAVKETGVT
                     KKEAERSKNMFALGVLSWMYSRPLEGTIGWLEKKFAKKPALAQSNTAALKAGHAFGET
                     AELSAFEVKPATLAPGTYRRITGNQALAYGLIAGSVQAKLPLFLASYPITPASDVLHE
                     LSRHKNFGVRTIQAEDEIAAAGMALGAAFSGHIGVTTTSGPGLDLKAETIGLAISVEL
                     PMIVVDVQRGGPSTGLPTKTEQSDLLQAMYGRHGESPLPIVAAYSPVSCFWAAIESCR
                     IALKYMTPVILLTDGYLANSAEPWRITSVGKLPDISVEFASQPNAGEDFWPFLRDETT
                     LARPWAIPGTAGLEHRIGGLEKADGTGNISYDPDNHHLMTQLRAAKIKVIEEDIEPLE
                     FDADEGADTLILGWGGTYGAIGAAVRRLRARGRKIARAHLKHLNPFPKNTEQVLRRFD
                     KIVIPEINTGQLARLIRAEYLIDVIQINKVRALPFRAGDLEQELLNIMGDK"
     gene            2021..>2890
                     /locus_tag="VNP73_01210"
     CDS             2021..>2890
                     /locus_tag="VNP73_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWL64567.1"
                     /translation="MAETEKALTGRGITEASSGVATLTRDDFASDQEVRWCPGCGDYA
                     VLAAVQSIMPSLGIPREKVVFVSGIGCSSRFPYYMNTFGFHGIHGRAPAIATGVAAAR
                     PDLQVWVVTGDGDALSIGGNHFIHALRRNVNLKILLFNNQIYGLTKGQYSPTSEQGKV
                     TKSTPFGSLEHPFNPVSLALGAEATFVARSIDVDRKHLPGMLSRAGAHRGSAFIEIYQ
                     NCNIFNDGAFSALTSKEGRTHNRIYLEHDKPIRFGPNDEKGVVMRSDGRAEIVDAKSV
                     SADQLLVHDEHRDD"
BASE COUNT          568 a          901 c          910 g          511 t
ORIGIN      
        1 cgcggggaaa tctccgaact gcgcgtcgac gacatcctgg cggcgaagtt cttcccgcga
       61 taggggcgcg accaggactt ttgcaccgag attcatgacc ccggcgttat agtgccgttg
      121 ttaaaagcga catcagcgcg cttcgtccag aggcaggcga tcaacacttg gcccaagctc
      181 caccggccgc ggaccgcaga gagttagaac gagtcgtcat ccgctttgcg ggggactccg
      241 gcgacggcat gcagctcacc ggagaccgat tcacatccgt cagcgccgta ttcggcaacg
      301 acctcgccac gtttcccgat taccccgcgg agatccgtgc cccggccggg acccttgcgg
      361 gcgtctcggc tttccagatc cacttcgccg actacgacat cctgactccc ggcgaccaac
      421 cgagcgtgtt ggtcgccatg aaccctgccg cgctgaaggc gaacctcaag gatctgatct
      481 cgggcgggct tctgatcgtg aacaccgacg ccttcgacga aaagaacctc gacaaggtcg
      541 gctatccctc caaccctctg aaggatggat cgctcggcga ctaccaggtc tacgagatcc
      601 cgatgacttc gatgacgatc gaggccgtca aagagaccgg ggtcacgaag aaggaggccg
      661 agcggtcgaa gaacatgttc gccctgggcg tgctgtcgtg gatgtactcg cgacctctgg
      721 aggggacgat cgggtggctc gagaagaagt tcgcgaagaa gccggcgctg gctcagtcga
      781 acaccgccgc actcaaagcg ggacacgcgt tcggagaaac cgccgagcta agtgcgttcg
      841 aggtcaagcc ggcgacgctg gcccccggga cctatcgcag gatcacgggt aaccaagccc
      901 tcgcttacgg gctgatcgcg ggaagcgttc aggccaagct gcctctgttc ctcgcctcgt
      961 acccgatcac gcccgcctcg gacgtgctgc acgagttgtc gcggcacaag aacttcggtg
     1021 ttcgaacgat ccaggccgaa gacgagatcg ccgccgccgg gatggcgctg ggtgcagcct
     1081 tctcggggca catcggagtg acgacgactt cgggcccggg tctcgatctc aaggccgaga
     1141 cgatcggact ggctatatcg gtggagcttc cgatgatcgt cgtcgacgtc cagcggggcg
     1201 gaccttcgac cggccttcca accaagaccg agcagtccga cctgctgcaa gcgatgtacg
     1261 gcaggcacgg cgaatctccg ctgccgatcg ttgcggccta ctcgccggtg tcgtgtttct
     1321 gggccgcgat cgagtcttgc cgcatcgctc tcaagtacat gacgccggtg atcctcctga
     1381 ccgacggata cctcgcgaac tcggcggagc cgtggcgcat cacgtcggtg gggaagctcc
     1441 ccgacatctc ggtcgaattc gcgtcgcaac ccaacgccgg cgaggatttc tggccgttcc
     1501 tgcgcgacga gacgacgctc gctcgtccgt gggcgatccc ggggaccgcc gggttggagc
     1561 accgcatcgg tggcttggag aaggccgacg gcaccggcaa catctcctac gaccccgaca
     1621 accatcacct gatgacccaa ttgcgcgccg cgaagatcaa ggtgatcgag gaggacatcg
     1681 agccattgga gttcgacgcc gatgaggggg ccgatacgct gatcctcggt tggggcggca
     1741 cctacggggc gatcggcgca gcggtcagga ggctcagggc ccggggtagg aagatcgctc
     1801 gcgcgcacct gaagcatctg aatccgttcc cgaagaacac cgagcaggtg ctgaggcgtt
     1861 tcgacaagat cgtgatcccc gagatcaaca cgggtcaact ggcgaggttg atcagagccg
     1921 agtacctgat tgacgtcatc cagataaaca aggtccgggc gttaccgttc cgggcggggg
     1981 atctcgagca ggagctcctc aatatcatgg gagataagta gtggccgaaa ccgaaaaggc
     2041 attaacgggt cgcgggatca ccgaagcctc cagcggggtc gcaacgctga cgcgcgacga
     2101 tttcgcatcc gatcaggagg ttcggtggtg tccagggtgc ggggactacg cggtgctcgc
     2161 cgccgtgcag tcgatcatgc cgtcattggg gatcccccgc gagaaggtcg tgttcgtgtc
     2221 gggcatcggc tgttccagcc ggttccccta ttacatgaat acgttcggtt tccacgggat
     2281 ccacgggcgc gctcctgcca ttgcgaccgg cgttgccgcc gctcgtcccg accttcaggt
     2341 gtgggtagtc acaggtgacg gggacgctct gtcgatcggc ggcaaccact tcatccacgc
     2401 gttacgacgc aacgtgaatc tcaagatcct gttgttcaac aatcagatct acggattgac
     2461 caaaggccag tactcgccga cgtccgagca gggcaaggta acgaagtcga cgccgttcgg
     2521 atccctcgag cacccgttca atccggtttc gcttgcattg ggggccgagg cgacgttcgt
     2581 ggcgcgttcg atcgatgtcg accggaaaca tctacccggg atgttgagtc gggcgggcgc
     2641 tcatcgcgga tccgccttca tcgagatcta ccagaactgc aacatcttca acgacggcgc
     2701 cttctcggct ctgacctcta aggagggacg cacgcacaac cggatctacc tcgagcatga
     2761 caagccgatc cggttcggac ccaacgatga gaagggtgtc gtgatgcgtt ccgacggccg
     2821 cgccgagatc gtggacgcga agagcgtctc ggcggaccag ctcctcgtcc acgatgagca
     2881 ccgcgacgat
//
LOCUS       DATMED010000050         3344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1577759, whole genome shotgun sequence.
ACCESSION   DATMED010000050 DATMED010000000
VERSION     DATMED010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577759"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VNP73_01215"
     CDS             <1..637
                     /locus_tag="VNP73_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64568.1"
                     /translation="DDEDDDDDDDDDTGGSGDSGSDDDDDSGHSGPGGDTRNTGEARA
                     ADHTNGTHDTGTGGTNEGDFTKDASRHSDGTAGTDGTAGTDGTDGGDNSVAAASVDTD
                     SVDTGTTNTGDTDDGDASVDAAALEGGVGAAGAEVGVVEAAAAPANGALGDQVLGSRI
                     ARGAAAPGAVDAAAEAEGGVLPFTGIQVGAMLAAAAGFIGSGSYLVRKNRR"
     gene            complement(689..2047)
                     /locus_tag="VNP73_01220"
     CDS             complement(689..2047)
                     /locus_tag="VNP73_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWL64569.1"
                     /translation="MTGIREFIEAHADEYVGWLTEACSIPSLAEKPEALTEMASWLQD
                     KLEMLGFDVARLELPGAPDTFLAEAGGGDRTLLVYDHYDVQPVDPIELWDSQPFQPEI
                     RDGKFFARGAADNKGDLIARLASLHAYRETKGNLPLRYKFLIEGEEEVGSKSFPALIA
                     RYGDRLKADGCIWEGMGIDHAGRPEFVFGAKGLAYIELWSRMLPDDQHSSLAVIAPSA
                     AWRLIEALRTLRDAEGNILIDGFYDDVVPPSERDEEILASLPFEEEAERARLGIEDFV
                     GNATGLDLVRRYFFEPTCNIAGLVSGFTIPGTEKTVLPKEAMAKIDLRLVPDQQPEEV
                     TAKIRKHLEQRGFDDIEVLYLSGQPPVRSEIDAAVGAATLAAARKVFAEPAAVSPLMI
                     GTGPMYPIAGTLGIPTVSPAGVCRPDSNIHAPNEHVLIDDFIDIVEYSATWIEEFAAT
                     TP"
     gene            complement(2078..2148)
                     /locus_tag="VNP73_01225"
     tRNA            complement(2078..2148)
                     /locus_tag="VNP73_01225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2114..2116),aa:Gly,seq:tcc)
     gene            2209..2604
                     /locus_tag="VNP73_01230"
     CDS             2209..2604
                     /locus_tag="VNP73_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL64570.1"
                     /translation="MSRSRKTAGEGTFHLIERKSNTVLIVDDDSDIRHALRLTFEWEQ
                     FEVVAEACDGIEAVRLAAQLRPAFVILDYYMPRMNGHKTSKLLREVAPGTKIVAFSSV
                     LEQKPRWADAFLNKEGISAAPVVLKELLV"
     gene            2643..2717
                     /locus_tag="VNP73_01235"
     tRNA            2643..2717
                     /locus_tag="VNP73_01235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2678..2680,aa:Pro,seq:tgg)
     gene            2972..>3344
                     /locus_tag="VNP73_01240"
     CDS             2972..>3344
                     /locus_tag="VNP73_01240"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HWL64571.1"
                     /translation="MSQALTTSTERIDKDRVRVRVEVAEDALKPAIDAAYKRLAQQIR
                     LPGFRKGKVPRQLIDQQLGPGAVRREAIEDALPSLLSDALRAEDLEAIAPAELDNIEG
                     VDDDSPLVFEATVDVRPEIPIP"
BASE COUNT          622 a         1035 c         1074 g          613 t
ORIGIN      
        1 tgacgacgag gacgatgacg acgacgatga cgacgacacc ggtgggtcgg gagattcggg
       61 atccgacgat gacgacgact cgggtcactc aggcccgggc ggggacaccc gtaatacggg
      121 cgaggctcgc gctgccgatc acacgaatgg cactcacgac accggaaccg gaggaacgaa
      181 cgaaggcgac ttcacgaagg acgcgagcag gcacagcgat ggaaccgccg gcacggacgg
      241 cactgccggg acggacggaa cggacggtgg cgacaactcc gtcgcggcag cctcggtcga
      301 tacggacagc gtcgacaccg gaacgacgaa caccggtgac acggacgatg gcgatgcttc
      361 ggttgatgct gctgcgctcg agggtggagt cggcgcggcc ggtgccgagg tcggcgttgt
      421 cgaagcagcc gccgcgcctg cgaatggggc gctcggcgat caggttctcg gttcgcgtat
      481 tgccagaggc gcggcagcgc ccggcgcggt cgacgctgct gccgaagcgg aaggcggtgt
      541 tcttcccttc acggggatac aggtcggcgc catgcttgca gcggccgccg gtttcatcgg
      601 atcgggttct tacttggtca ggaagaatcg ccggtaacac ggtacgaggg gataagcgac
      661 gaggggcccg agcggcccct cgttcgcgtt atggggttgt cgctgcgaac tcttcgatcc
      721 aggtcgcgct gtactcgacg atgtcgatga agtcgtcgat cagcacgtgc tcgttcggcg
      781 cgtggatgtt cgagtctggt ctgcatacgc cggcgggaga gacggtcggg attccgagag
      841 ttccggcgat cggatacatg ggccccgtgc cgatcatcaa cggcgacacc gcggccggct
      901 ccgcaaaaac cttcctcgcg gcagcgaggg tcgcggcacc gacggccgca tcgatctcgg
      961 atctgaccgg tggctgcccg ctgagataga gaacctcgat gtcatcgaag ccgcgttgct
     1021 ctagatgctt cctgatcttc gctgtgacct cttcgggttg ttgatcgggg acgagcctca
     1081 gatcgatctt ggccatcgct tcttttggaa gcacggtctt ttcggttccg gggatcgtga
     1141 accccgagac cagccccgcg atgttgcacg tcggttcgaa gaagtagcgg cggaccaggt
     1201 cgaggccggt ggcgttccct acaaaatctt cgatgcccag tcgggcgcgc tcggcttctt
     1261 cctcgaacgg cagcgacgcc aggatctcct cgtcccgctc gctcgggggc acgacgtcgt
     1321 cgtagaagcc gtcgatcagg atgttgcctt ctgcgtctct cagcgtgcgc aatgcctcga
     1381 tcagtctcca ggcagcactc ggtgcgatga ccgcgagcga cgagtgttga tcgtccggga
     1441 gcatgcgaga ccacagttcg atataggcga gacctttggc gccgaacacg aactccggcc
     1501 tgcccgcgtg atcgatgccc attccctccc agatgcatcc gtcggccttc agccgatcgc
     1561 cataacgggc gatgagggcc gggaaacttt tggagccaac ctcttcctcg ccctcgatca
     1621 ggaacttgta gcggagaggg aggtttcctt tcgtctctcg gtacgcatgc agcgacgcga
     1681 gccgggcgat caggtcaccc ttgttgtcgg ccgctcctcg tgcgaagaac ttgccatcac
     1741 ggatctccgg ttggaacggt tgggagtccc acagctcgat cggatcgacg ggttgcacgt
     1801 cgtaatggtc gtagacgaga agggtgcgat ctccgccccc ggcctccgcg aggaacgtgt
     1861 cgggagcgcc gggaagctcg agccgggcca cgtcgaagcc gagcatctcg agcttgtcct
     1921 gaagccatga ggccatctcg gtgagagcct cgggcttctc tgcgagggag gggatcgaac
     1981 aagcctccgt gagccacccg acgtactcgt cggcatgagc ctcgatgaac tcccggatcc
     2041 ctgtcacacc ccagtcatac ctcgcataga caagcagagc gggtgagggg aatcggaccc
     2101 ctgtcacttg cttggaaggc aagagctcta ccattgagct acacccgcgt gatcccactt
     2161 taaaacctca accgatgggt ctagtctttc caacttcagg gttcagagtt gagccgatcg
     2221 cgaaagactg ctggggaagg cacctttcac ttgatcgagc ggaagagcaa taccgttcta
     2281 atcgtcgacg acgactccga catccggcac gccctcagac tcacgttcga gtgggagcaa
     2341 ttcgaagtcg tggcggaggc ttgtgacggc atcgaagccg tcaggctcgc ggcccaactc
     2401 cggcccgcgt tcgtgatcct cgactactac atgcctcgta tgaacggcca caagacgtcg
     2461 aagctcctac gagaggtggc ccccggcacg aagatcgtgg ctttctcgtc agttctcgag
     2521 cagaaacccc gctgggcgga cgcgttcctc aataaggagg ggatctcggc ggcccccgtc
     2581 gtactcaaag agctactggt ctagaccagg cgactcgacg gccctggcta gactggctcc
     2641 gacggggcgt agcgcagctt ggttagcgcg cggcgtttgg gacgccgagg tcggcggttc
     2701 gaatccgccc gccccgacga ttactaggga ggggccgcgg tctacccttg tatgattaag
     2761 cagaacaagg gtggggcagc tgggcgcggt tccggttggg agccaccagc cgtcatcccc
     2821 actttcgtcc gcctagcgcg gttggtggct cccaacggga acctcgaacg gctttgtttc
     2881 ccgcgaattg ccttgatgta gttgttccac atcgtttgtc ctagtccttc ctcatttttc
     2941 gacgtacccc ccgcatagga tttcgtcagc tatgagccag gccctcacca cctccaccga
     3001 gcgcatcgac aaggaccgcg tccgtgtccg cgttgaggtc gccgaagacg ccctcaagcc
     3061 cgccatcgac gccgcctaca agcgtctcgc ccagcagatc cgccttcccg ggttccgcaa
     3121 gggaaaggtg ccccggcaac tgatcgatca acagctcggg ccgggggccg tcaggcgcga
     3181 agcgatcgag gacgcactgc cttctctact gagtgacgct ctgcgcgccg aggacctaga
     3241 agccatagcc cctgccgagc tggacaacat cgagggggtg gacgacgaca gcccgctggt
     3301 cttcgaggca acggtggatg tgcggcccga gataccgatc ccgg
//
LOCUS       DATMED010000051         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3113128, whole genome shotgun sequence.
ACCESSION   DATMED010000051 DATMED010000000
VERSION     DATMED010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3113128"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VNP73_01245"
     CDS             complement(<1..872)
                     /locus_tag="VNP73_01245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWL64572.1"
                     /translation="MKAYRLRGPCSMSGEVPISGAKNSALKLMAASLMIPGKVTLHRV
                     PRIRDVHLMAEVLERLGAHVTFEGNDLTVDASGDLAEETPYELVTKMRASIQVLGPLL
                     ARLGRARVAMPGGDAIGSRPIDLHLLGLERMGAKFVTEHGYVEGSCGRLQGTSVLLEF
                     PSVGATENLIMAGVLAEGTTKIENAAREPEIQDLVAFLQGCGAQISGAGTPSIEIEGV
                     DLLGPTEHEIIPDRIEAGTYAIGVAAGRGDVTLVDMRPEHLELPLQKLEQAGAEVRRG
                     DGEVRVAMGTRPHS"
     gene            complement(966..1319)
                     /locus_tag="VNP73_01250"
     CDS             complement(966..1319)
                     /locus_tag="VNP73_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64573.1"
                     /translation="MAEEEKSLVDKARDLTAGLYDTAADVVAPEGGDTGAVATARRAT
                     ASGAETVAEVARPGRTSAARKSSGRRKSTASRGRKKTTGRKKSTARKKTTGRKKSTAR
                     RKPAARKKTTRRRKR"
     gene            1449..1928
                     /locus_tag="VNP73_01255"
     CDS             1449..1928
                     /locus_tag="VNP73_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64574.1"
                     /translation="MQQKLLNIYLNDHLAGAAAGLELAKRCRSNNIGTPLGSFLDGLI
                     ADIEADKDELEALMSALDAPTDRLKQAAAWLGEKGGRLKLNGQITGYSPLSRLIELEG
                     LCLGVDGKSSLWRSLMQISGHDARIATFDLDRLEKRAIEQREELEVHRQEAARTAFA"
     gene            complement(2083..2292)
                     /locus_tag="VNP73_01260"
     CDS             complement(2083..2292)
                     /locus_tag="VNP73_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64575.1"
                     /translation="MGDSVGDLRSRVYCSYCGASESRRHGLTPMGWQEMPDANNRPIA
                     ICPRCTRDNLWLIEGRLDLDPGSGI"
     gene            complement(2302..2691)
                     /gene="atpC"
                     /locus_tag="VNP73_01265"
     CDS             complement(2302..2691)
                     /gene="atpC"
                     /locus_tag="VNP73_01265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HWL64576.1"
                     /translation="MKVSIVTPEKAMWEGEADLVIARSPEGEFGIMRGHIPFLAALVP
                     GLITVVEGGGRQQYIVPGGFLEASGPVDDYHVIVLADDAEEAGDIDAAEAIKRIEQAQ
                     REKDEDVDQHAEAKMRAAMAGPQLRRD"
     gene            complement(2715..>3617)
                     /gene="atpD"
                     /locus_tag="VNP73_01270"
     CDS             complement(2715..>3617)
                     /gene="atpD"
                     /locus_tag="VNP73_01270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWL64577.1"
                     /translation="IYRVAEQHSGVSVFAGVGERTREGTDLLIEMKESGVIDKTALVY
                     GQMDEPPGVRLRVALSALTMAEYFRDEEHQDVLLFVDNIFRFVQAGSEVSTLLGRMPS
                     AVGYQPTLSEEMGELQERITSTKGRSITSLQAIYVPADDITDPAPHTTFAHLDATTVL
                     SRAISELGIYPAVDPLDSTSRILDPRYVGQEHYDVALQVQRSLQRYKDLQDIIAILGI
                     DELSEEDRITVQRARKIQRFLSQPFFVAEQFTGIPGKYVSKEETVESFKGLVEGEYDD
                     LPEQAFYMVGGIEEAQAKAKELNE"
BASE COUNT          652 a         1163 c         1106 g          696 t
ORIGIN      
        1 atcgagtgcg gtctcgtacc catagcgacg cgcacctcac cgtcgccgcg gcgaacctcg
       61 gcgcccgcct gctcgagctt ctgcaacggc agttcgaggt gttccgggcg catgtcgacg
      121 agagttacgt cgcctcgacc cgccgcgacg ccgatcgcgt aggtccccgc ttcgatgcgg
      181 tccgggatga tctcgtgctc ggtcggaccc aacaagtcga caccttcgat ctcgatcgag
      241 ggcgtcccgg cgccgcttat ctgcgcaccg catccctgga ggaacgcgac gagatcctgt
      301 atctccggct cgcgggcggc gttctcgatc ttcgtcgtac cttccgcgag taccccagcc
      361 atgatgaggt tctcggtcgc gccgaccgac gggaactcga ggagaacgct ggtgccttgc
      421 agacgaccac acgacccctc gacatagccg tgttcggtga cgaacttcgc acccatccgc
      481 tcgagcccaa ggagatgtag atcgatcggt cgggatccga tcgcgtcccc tccgggcatc
      541 gccacacggg cgcgccccaa tcgagccaga agggggccga gaacctggat cgaagcgcgc
      601 atcttggtga ccagctcgta gggggtctct tccgcgagat cgcccgatgc gtcgacggtg
      661 agatcgttgc cctcgaaggt gacgtgggca ccgagtcgtt cgagcacctc ggccatcaga
      721 tgaacgtcgc ggatgcgcgg gacgcgatgc agcgtcacct tgccggggat catgagcgac
      781 gcggccatca gcttgagggc cgaattcttt gcgccggaga tcggcacttc gccggacatg
      841 ctgcacgggc cccgcagccg gtaagccttc attgtgtgaa tcctaggtgg gggtaggggc
      901 ccccagcgcg tagcgaaaaa gcggcccccg taggggccgc tttcctagta ctcggtactc
      961 ggtcgctagc gcttgcgcct gcgtgtggtc ttcttgcgag ccgccggctt cctgcgcgcc
     1021 gtggacttct tgcgacccgt ggtcttcttg cgagccgtcg acttcttacg accggtcgtc
     1081 ttcttgcggc cacggctggc cgtgctcttg cgacgaccgc ttgacttacg ggctgcggaa
     1141 gtgcggcccg gacgtgctac ttcggctacg gtctcggccc ccgatgccgt cgcacgacgt
     1201 gccgtcgcaa ccgcaccggt atcaccgccc tcgggcgcaa ctacgtccgc agcagtgtcg
     1261 tacaggccgg cagtgagatc tctcgcctta tcgacgagac tcttctcctc ttctgccata
     1321 tgcatccccc ttccctttgt ttcgatgacg cactcgtttt acttacccac aaacctgctc
     1381 tgtttacacg agagcacgtt aggaaacaag tgtgactacc acacaaaact gcgagaggac
     1441 aaatcgaaat gcagcagaaa ctgctcaaca tctatctgaa cgatcatctc gcgggagctg
     1501 cggcgggtct tgaactagcg aaacgatgcc gttcgaacaa catcggaaca ccgttgggtt
     1561 cgttcctcga cgggttgatc gcagacatcg aagcggacaa ggacgagctc gaagcgctca
     1621 tgtctgcact ggacgcgccc accgatcgac tgaaacaagc ggccgcgtgg ttgggcgaaa
     1681 agggtggtcg gctgaagctg aacggacaga tcacgggcta ttcaccgctg agccgcttga
     1741 tcgagcttga aggactgtgc ctgggcgtgg acgggaagtc atcgctgtgg cggtcactca
     1801 tgcagatatc gggacacgac gctcgtatcg ccacattcga cctcgaccgg ctcgagaagc
     1861 gagcgatcga acagcgcgag gaactcgagg ttcaccggca agaggccgcc cgcaccgcct
     1921 tcgcctgatc caccaaagtg aaagccggcc cgaaggccgg cttttgtgta gctcagttca
     1981 gatggtgact actcgcactc cttgacgcaa cgacgcccag gtcctcccag atagatcgac
     2041 gtccacccca aacgtcttcg ctgccgaacc cggcaacctc cctcagatgc cggagcccgg
     2101 atccagatcc agtcggccct cgatcagcca caggttgtcc cgagtacatc taggacagat
     2161 ggcgatcgga cggttgttcg cgtcgggcat ctcctgccat cccattggcg tgagcccatg
     2221 acgccgggac tcgctggcgc cgcagtagga gcagtagacg cgactcctta gatcccccac
     2281 cgagtcgccc attcgacaaa cttagtctcg gcggagctgc ggtccggcca tcgcggctcg
     2341 catcttggct tccgcgtgct gatcgacgtc ttcgtctttc tcgcgctgag cctgctcgat
     2401 ccgtttgatg gcctcggcgg cgtcgatgtc accggcctcc tcggcatcgt ccgcaagaac
     2461 gatcacgtga tagtcgtcga ccgggccgct cgcctccagg aagccgccgg gcacgatgta
     2521 ctgctgcctg ccgccccctt cgacaacggt gatcaggcct ggcacgagcg cagctagaaa
     2581 tgggatgtga ccgcgcatga tgccgaactc gccttccggc gagcgcgcta taacgaggtc
     2641 cgcttcgccc tcccacatgg ccttctcggg agtgacgatg cttaccttca tattgccggc
     2701 catgtctgtc ctcgctactc gttgagctct ttggctttgg cttgcgcttc ctcgatgccg
     2761 ccgaccatgt agaaagcctg ctcgggaaga tcgtcgtact caccctcgac gaggcccttg
     2821 aagctctcga ccgtctcctc tttgctcaca tacttgccgg ggataccggt gaactgctcg
     2881 gcaacgaaga acggctgaga aaggaagcgc tggatcttgc gggcacgctg aacggtgatg
     2941 cggtcttctt ccgacagctc gtcgataccg aggatggcga tgatgtcctg gaggtccttg
     3001 taacgctgga ggctccgctg cacctgcaag gcgacgtcgt agtgctcttg gcccacgtat
     3061 cgggggtcaa ggatccgcga ggtcgagtcc agcgggtcaa cggcggggta gatgcccagc
     3121 tcggagatcg ctcgggacaa caccgtcgtg gcatcgaggt gagcgaacgt cgtgtggggg
     3181 gccgggtcgg taatgtcgtc ggccggcacg tagatggctt gcagagaggt gatcgaacga
     3241 cccttggtcg aggtgatgcg ctcctgcaac tcgcccatct cttccgacag cgtcggttgg
     3301 taacccacgg ccgaaggcat ccggccgagc agtgtggata cctcggaacc ggcctggacg
     3361 aaccggaaga tgttgtcgac gaataggagc acgtcctggt gctcttcgtc acggaagtac
     3421 tccgccatcg tgagcgccga cagggcgaca cggagccgca cgcccggggg ctcgtccatc
     3481 tggccataca cgagcgccgt cttgtcgatg acgcccgact ccttcatctc gataaggagg
     3541 tcggtgccct cacgggtgcg ctcgccgacg ccggcgaaca cggagacacc ggagtgctgc
     3601 tcggcgacgc ggtagat
//
LOCUS       DATMED010000052         7755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3882928, whole genome shotgun sequence.
ACCESSION   DATMED010000052 DATMED010000000
VERSION     DATMED010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3882928"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..667)
                     /locus_tag="VNP73_01275"
     CDS             complement(308..667)
                     /locus_tag="VNP73_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64578.1"
                     /translation="MGFGDRIRGFFSRRNNPDLARLEDFARTHQGVEGYVEPLTATQP
                     TTLLLVDRVGAHVRGAVADPRDAFSFCEGLGLPVYDAAVIGYPKRMKDFAGGRKADPS
                     ALDEQIADLERRLSEPE"
     gene            complement(690..1649)
                     /locus_tag="VNP73_01280"
     CDS             complement(690..1649)
                     /locus_tag="VNP73_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWL64579.1"
                     /translation="MAKVWVYAERDGQGNVDPAALEILTKAKSLGGDVEAVILGAGAT
                     DAAKELGQYGATTVHASDDAVYDDYLVLPHVQALASLCEQHSPDAILFAMNYDSRDIA
                     GRLSARLGSTLMSNAMDIEGTETAKTAIFGGAQIVDVKLEGSPKLVLFRPKSFEAKAA
                     GGEATVASVEVEIPEDAKKSKIVERHEEAASGPKLEDAPVVISGGRGLQEPDNFKLLD
                     DLASAIGGAAVGATRAVVDAGWVPYAMQVGQTGKTVKPGVYIAVGISGATQHVVGMKG
                     AKKIIAINKDEEAPIFQLADLGIVGDALKVVPQLIEEIKTRKG"
     gene            complement(1650..2417)
                     /locus_tag="VNP73_01285"
     CDS             complement(1650..2417)
                     /locus_tag="VNP73_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWL64580.1"
                     /translation="MNIAVCVKHIPDPNLPPEMDGDTLKREGVQGVLDPGDEFGVEAA
                     LQLKEQHGGEVTLVSMGPAQAMEAIRRGLSMGADKGVLVSDDSLKGSDALVTAKVLAE
                     TIKKQGEFDLIITAVESTDGYTGTMAATLAEFLGLPQVTFVKKIEGSNGAIKVDRQTA
                     DGYHVVESSLPALISVTAGVNEPRYASFKGIMAAKKKPVEELSLSDLGLSGDDVAVKQ
                     SIAQMAPAEERKAGEVIEDDGTGAAKIADFLKEAKVI"
     gene            complement(2519..3475)
                     /locus_tag="VNP73_01290"
     CDS             complement(2519..3475)
                     /locus_tag="VNP73_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL64581.1"
                     /translation="MNELPVTRIETPTPWSGIGPVNSYLVEAEPVTLVDSGFNSPQGQ
                     NALRLALADRHLFLESVERILVTHGHPDHYGLVSMIQDAAGARVYFPASEIKRVIDPE
                     MLFRTGRLLVEAGMPLEMLFKMSKQRGQQPGPRVDHEQVIPVNDGDRFAFEGFELVAL
                     HMPGHTGGHFVYFEPQTRTLFAGDQLLPKTSPNPLLEPSLDDPEERRRSLKDYLGSLQ
                     KMAGMDLTLVYPGHGDPVTDPGELIQSTIEHHDKRKVEVAGFLGAESQSPFQIAERMF
                     PDKMQYEAYLAVSEVVAHLDLVVDDGDAQVSEDEGVTYYRSA"
     gene            complement(3472..4251)
                     /locus_tag="VNP73_01295"
     CDS             complement(3472..4251)
                     /locus_tag="VNP73_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWL64582.1"
                     /translation="MGEFVNLEVADGVGTIRLSRGKLNPINAQVVQEINEAIEAAETD
                     DNVRAVVVWGGENVFAAGADINEMEDRDTISMFRYISGLQDVFTRLEHLPVVTIAAIN
                     GFALGGGCELALACDLRICAEDSQLGQPEILLGIIPGAGGTQRLPRLIGVGRAKDLIY
                     SGRFVKADEALTMGLVNKVVSAADVYSTAVKWAQRYASGPTVALLAAKQVIQNGIDTD
                     MATGQLLERQAFAALFSSEDQKIGMKSFIEEGPGKAKFVGR"
     gene            4307..4936
                     /locus_tag="VNP73_01300"
     CDS             4307..4936
                     /locus_tag="VNP73_01300"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWL64583.1"
                     /translation="MEDFGTRLAALLGAREPQRVEMAEARDAAVLIPLIATPEPTLIF
                     TVRTDTLPSHKGQIAFPGGSIDDTDASAEAAALREAEEEIGLDPDRVRILGELDTTPT
                     FVSGYVIYPFVGWLEEQPDLDPNPAEVAEVLLVPLSELVEDIRREPGFAHGGRTFPTE
                     AWVWRDHVIWGVTARIIRHFLVLLGEAGLAATPGPTDSWTAWPLTSTRS"
     gene            4933..5103
                     /locus_tag="VNP73_01305"
     CDS             4933..5103
                     /locus_tag="VNP73_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64584.1"
                     /translation="MSWITFAATHTPSPTSTIVQPPAAGTFETVAAALAIVLAVGAGI
                     LGYRIIRGGRGL"
     gene            5139..5876
                     /locus_tag="VNP73_01310"
     CDS             5139..5876
                     /locus_tag="VNP73_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64585.1"
                     /translation="MALVLSLSLIAACSDTGPDAGDRTLLGTETPRAGKDKGKKPGGK
                     NAERKKGGNNRGSGSAGKDGGTVAGGPSALPEQVDVPGADEDKTYPTASAEIVESKPD
                     GNGQGITPGYAELLGTRVEGLGKDFRVTLTFNGEVPQEMPNDKSIMVIGFQVLRGRDD
                     GYAFSGQATEKGWQPYAGGKEKATDFPGRFEVSGSTIVMEIPWSYVEGAYPFKWLVTS
                     NWYQSLANTTHYIFDLIPNKGQANYPG"
     gene            complement(5877..7529)
                     /locus_tag="VNP73_01315"
     CDS             complement(5877..7529)
                     /locus_tag="VNP73_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWL64586.1"
                     /translation="MADDQQRRWREGYEQSKLRDDIDFDTLSSVELDPVYGPEEGVDP
                     RVGVPGEYPFTRGIYPTMYRGRLWTMRQFAGFGSPQQTNERFKFLVEHGQTGLSVAFD
                     MPTLMGRDSDDPYSEGEVGRCGVAIDSLADMEILFADLDLEALTTSMTISGPAPMLFA
                     MYIAAAEKQGADISKLDGTCQTDILKEYIAQKEWLFPPEPHLRLTADMMEYCSTDVTK
                     YHPLSISGYHIREAGSTAVQELAFTVASGFAHVELGLRRGLDIDEFAPGLSFFFNSHV
                     DFFEEIAKFRAARRIWARWMKERYGAKKERSWMLKFHTQTAGVSLTAQQPYNNVVRTA
                     TEALAAVLGGTQSLHTNSLDEVLALPTDESVEIALRTQQVIGYETGVTNVIDPLGGSW
                     FVEALTDKTEAAAEEYFTKIDEMGDGSILDGMLKGIEEGYFQKEMADAAFRYQQLLER
                     DKKVMVGVNRFTQTVQAPPDVLVIDPEIEEKQKKALAEVRANRDAKRAEDAIQALRDM
                     AHDEKQNSIPVMAEAAKAYVTLGEMVEALKQEWGVYTEPPMF"
BASE COUNT         1423 a         2481 c         2343 g         1508 t
ORIGIN      
        1 tcgtcgctcc gactgttgcc ggtgtcgtga ccgtcccggt gatgagcgaa agccggggcg
       61 ctcatgagat atccaaaagt ggtcagaagt gctatcagca acatcagcca cgagatccgc
      121 ttgatcttga tcatttcccg ccgtcctccc tactgcctac tctgtgcagt cgtccgccgt
      181 ctccctgcat tctaaccccg cgtttgctcg cttaattcaa cgacaaacag ggggaaaagg
      241 atacacacgc atgggtcgaa tttccgattt ctgccacatt tacgcaggat tacgtatgcg
      301 attctggcta ttccggctcg ctcaggcggc gctcgaggtc ggcgatctgc tcgtcgagag
      361 ccgacggatc cgctttccgg ccccctgcga agtccttcat ccgcttcgga tagccgatca
      421 cggcggcgtc gtagacgggc agacccaggc cttcacagaa ggagaaggcg tcgcgagggt
      481 ctgcgaccgc ccccctgaca tgcgctccga cccggtcgac cagaagcaag gtcgtgggct
      541 gcgtcgccgt caggggttcg acgtaaccct caaccccctg atgggtccga gcgaagtcct
      601 caaggcgagc caggtccggg ttgttcctac gtgagaagaa ccctctgatc cgatccccga
      661 aacccatggc cggactttcc taacaccgat cagcccttac gggtcttgat ctcctcgatg
      721 agctggggaa cgaccttcag cgcgtccccg acgatgccga gatcggcgag ttggaagatc
      781 ggcgcctctt catccttgtt gatggcgatg atcttcttgg cgcctttcat cccgacgacg
      841 tgctgcgtcg ccccggagat gcccacggcg atgtaaacgc ctggcttcac ggtcttaccg
      901 gtctggccta cctgcatcgc gtagggaacc cagcccgcgt cgaccactgc gcgggtcgcg
      961 ccgacggcgg ccccgccgat cgcagaagcg aggtcgtcca gcagcttgaa gttgtccggt
     1021 tcctggagac cccggccgcc ggagatcacg acgggcgcat cttcgagctt cggcccgctg
     1081 gctgcttcct cgtgacgttc cacgatcttc gacttcttgg cgtcttcggg gatctcgacc
     1141 tcgactgaag cgacagttgc ttcgccgccg gccgccttcg cttcgaatga cttcggacgg
     1201 aagagaacca gcttgggaga gccttcgagc ttcacgtcca cgatctgagc cccaccgaag
     1261 atcgcagtct ttgccgtctc cgtcccctcg atgtccattg cgttgctcat gagggtcgac
     1321 ccgagccggg ccgacaaccg gccggcgatg tcgcgcgagt cgtagttcat ggcgaacagg
     1381 atcgcgtcgg gcgagtgctg ctcgcacagt gacgccagcg cctgaacgtg cgggagcacg
     1441 agatagtcgt cgtaaacggc atcatcgctc gcatgaacag tcgtcgcgcc gtactgcccg
     1501 agctctttcg cagcgtcggt cgcacctgcc ccgaggatga ccgcttcgac atcgcccccc
     1561 agggacttcg ccttcgtgag gatctcaagc gccgccggat cgacgttgcc ctggccgtca
     1621 cgctctgcgt aaacccagac ctttgccacc tagatcacct tcgcttcttt gaggaagtcc
     1681 gcgatcttcg cggctccagt gccgtcgtct tcgatcactt caccggcctt gcgctcctcc
     1741 gccggagcca tctgagcgat cgattgcttc acggcaacgt catcgccgct caagcccagg
     1801 tcggacagcg atagctcctc gaccggcttc ttcttcgccg ccatgatgcc tttgaacgat
     1861 gcataacgag gctcgttgac accggcagtc acggagatca acgccggcaa cgagctctcg
     1921 accacgtggt acccgtcggc cgtctgcctg tcgactttga tcgccccgtt ggatccctcg
     1981 atcttcttca cgaaggtcac ctggggaagc ccgaggaact cggccagagt cgcggccatc
     2041 gtcccggtgt aaccgtcggt cgattcgacc gccgtgatga tcagatcgaa ctcaccctgc
     2101 ttcttgatcg tttcggcgag cacctttgcc gtcacgagtg cgtcggaacc cttgagagaa
     2161 tcatccgaaa cgaggacgcc tttgtcggcc cccatggaca gacccctgcg gatcgcctcc
     2221 atcgcttggg cgggacccat cgagacgagg gttacctcgc caccgtgctg ttccttgagc
     2281 tgaagggccg cctcgacccc gaattcgtcc cccggatcca gcacgccctg aacgccttca
     2341 cgcttgagcg tgtcgccgtc catctcgggc ggcaggttcg ggtccgggat gtgcttcaca
     2401 cagaccgcga tattcacaac gaacctccta gaaagacctg cgagaaagtc gccactggtc
     2461 gctcgccata gcgctgctag ctatagcaag caccctaccc caaacgcacc aggccgaatc
     2521 aagccgagcg atagtaggtc accccctcgt cctccgagac ctgggcatcg ccgtcgtcca
     2581 cgacgaggtc gaggtgcgcg accacctcgg acacggccag gtaggcctcg tattgcatct
     2641 tgtcggggaa catccgctcg gcgatctgga acggactctg gctttcggct cccaggaacc
     2701 ccgccacttc gaccttgcgc ttgtcgtggt gctcgatcgt cgactggatc agctcgcccg
     2761 ggtcggtgac cggatcgccg tgacccggat aaacgagggt gaggtccatc cccgccatct
     2821 tctggagaga gccgaggtag tccttgaggg acctccggcg ctcctcggga tcgtcgagcg
     2881 acggctccag cagggggttg ggtgaggtct tgggtagcag ctggtcgccg gcgaacagcg
     2941 tccgagtctg aggttcgaag tagacgaaat ggcctccggt atggcccggc atgtgcaacg
     3001 cgaccaattc aaaaccttcg aatgcgaagc ggtctccgtc gttaactgga atgacctgtt
     3061 cgtgatccac gcgtgggccc ggttgttgcc cgcgctgttt gctcatcttg aacagcatct
     3121 caagaggcat cccggcttcg acaagcaacc gaccggttct gaacagcatc tcgggatcga
     3181 tcacgcgctt gatctcgctc gcagggaagt aaaccctggc gccggccgcg tcctggatca
     3241 tcgaaacaag tccgtagtgg tccggatggc cgtgcgtcac gagtatgcgc tccacggact
     3301 cgaggaaaag gtgacggtcg gccagcgcaa gtctcaaagc gttctgaccc tgcgggctgt
     3361 tgaaacctga atcaacaagc gtgacgggct cggcttcgac caggtaggag ttgactggtc
     3421 ctatccccga ccatggggtc ggagtctcga ttcgtgtgac cgggagttcg ttcaacggcc
     3481 gacgaacttc gcctttcccg ggccctcttc gatgaagctc ttcatcccga tcttctgatc
     3541 ctcggaggag aagagagccg cgaaggcctg gcgctcgagg agttgaccgg tcgccatgtc
     3601 ggtgtcgatc ccgttctgga tcacctgctt ggcggcgagc agggcaacgg tcggccccga
     3661 cgcatagcgc tgagcccact tcacagcagt cgagtaaacg tcggccgcgg atacgacctt
     3721 gttgaccaat cccatcgtca aggcctcgtc ggccttcacg aagcgcccgg agtagatcag
     3781 gtccttcgcc cgaccgacgc cgatcagacg cggcaaacgt tgcgtgcccc ctgccccggg
     3841 gatgatcccg agcaggatct ccggctggcc caactgagag tcttccgcgc agatgcgaag
     3901 gtcgcacgca agagcgagct cacatccacc gccgagtgca aacccattga tcgctgcgat
     3961 cgtcaccacg ggcagatgct ctaggcgcgt gaacacatcc tggaggccgg agatgtaacg
     4021 gaacatcgag atggtgtcgc gatcctccat ctcgttgatg tcggctcccg cggcgaagac
     4081 gttctcgcct ccccacacga cgacggcgcg cacgttgtcg tcggtttccg ccgcttctat
     4141 cgcctcgtta atctcttgca cgacttgggc gtttatcggg ttcagcttgc ctcggctgag
     4201 acggatcgtc ccgacgccgt ctgccacttc gagattcacg aattcaccca tctcggctcc
     4261 tcgcctagga atcgttaatg cgaccgaagc ctaagatgac tcgatcatgg aggacttcgg
     4321 gacgcgtctg gccgcgcttc tgggcgcaag agagccgcag cgggtagaga tggccgaggc
     4381 gcgcgatgcc gcggtactga tcccgctgat cgcaacgccc gaaccgacct tgatcttcac
     4441 cgtgcgaacg gacaccctgc ccagccataa gggccagatc gcattccccg gcgggtccat
     4501 agacgacacc gatgcttctg ccgaggcggc ggctcttcga gaagcggagg aggagatcgg
     4561 cctcgacccc gaccgcgtac gcatcctcgg tgagctcgat acgactccca cattcgtatc
     4621 gggctacgtg atctatccct tcgtgggctg gctggaagaa cagccggacc tcgaccccaa
     4681 cccggctgag gtcgccgagg tacttctcgt cccactgagc gagctggtcg aagacatccg
     4741 gcgcgagccc ggcttcgcgc atgggggccg gaccttcccg actgaggcct gggtctggag
     4801 ggaccacgtg atctggggag tgacggcgcg catcatcagg cacttcctcg tccttctcgg
     4861 cgaagcgggc ctggcggcaa cacccggacc caccgattct tggaccgcgt ggccgcttac
     4921 gagcactcga tcatgagttg gatcacattc gcggcgacgc atacaccctc gccgacatcg
     4981 accatcgttc agccccccgc tgcggggact ttcgagacgg tggcagcagc gctcgcgatc
     5041 gttctggcgg taggcgctgg gatactcgga tacaggatca tccgaggagg acgagggttg
     5101 tgagatccgc agcgcgcagc tcgaggtcat ccgttgcaat ggcactcgtc ctctcgctct
     5161 cgttgatcgc cgcgtgttcc gataccggtc ccgatgcggg cgaccgcact ctgttgggca
     5221 ccgaaacccc gcgggccggc aaggacaaag gcaagaaacc gggtggcaag aacgcggagc
     5281 gaaagaaggg cgggaacaac agaggatccg gttctgccgg caaggatggg gggaccgtag
     5341 ccggagggcc atcggcactt cccgaacaag tcgacgtccc aggagccgac gaggacaaga
     5401 cgtatccgac ggcgtcggcc gagatcgtcg aatcaaagcc cgacggcaat ggccagggga
     5461 tcaccccggg atacgcagag ctgcttggaa ctcgggtcga gggactcggc aaagacttcc
     5521 gcgtgacgct caccttcaac ggtgaagtgc ctcaagagat gccgaacgac aagtcgatca
     5581 tggtcatcgg attccaggtg ttacgggggc gcgacgacgg ttatgcgttc tccggacaag
     5641 caacggagaa aggctggcag ccgtacgccg ggggcaagga gaaggccacc gatttcccgg
     5701 gtcgattcga ggtcagcggt agcacgatcg tcatggagat cccatggtcg tacgtcgagg
     5761 gggcctaccc gttcaagtgg ttggtgacgt cgaactggta ccagtcactc gcgaatacca
     5821 cgcactacat cttcgacctg atcccgaaca agggccaggc gaactacccg ggttgactag
     5881 aacatcgggg gttcggtgta gacgccccat tcctgcttga gagcctcgac catctctccc
     5941 agcgttacat aggccttcgc agcttcggcc atcacgggga tcgagttctg cttctcgtcg
     6001 tgggccatgt cgcgcagtgc ctggatcgcg tcctcggccc tcttcgcatc cctgttcgcc
     6061 cgcacctcgg ctagggcttt cttctgcttc tcctcgatct ccggatcgat cacgagcacg
     6121 tccggggggg cttgcacggt ttgcgtgaac cgattcacgc cgaccatgac cttcttatcg
     6181 cgctcgagta gttgctggta acggaacgca gcgtcggcca tctccttctg gaagtagccc
     6241 tcttcgatcc ccttgagcat cccgtcgagg atcgagccat cccccatctc gtcgatcttg
     6301 gtgaagtact cctcggccgc agcttccgtc ttgtcggtga gagcctcgac gaaccacgat
     6361 ccgccgagcg ggtcgatcac attcgtaacg ccggtctcgt acccgatcac ctgttgggtg
     6421 cgaagcgcga tctcgacgga ctcgtcggta gggagggcca gcacctcgtc gagcgagttc
     6481 gtgtgcagac tctgggttcc gccgagcacc gcggcaagag cctcggtggc agtgcggacg
     6541 acgttgttgt acggctgctg cgcggtcaga gacacgccgg cggtttgggt gtggaacttg
     6601 agcatccagg agcgttcctt cttcgctccg tagcgctcct tcatccaccg cgcccagatc
     6661 cggcgagcgg cacggaactt agcgatctcc tcgaagaagt cgacgtgcga gttgaagaag
     6721 aacgaaagcc ccggcgcgaa ctcgtcgatg tcgaggccgc gacgcagtcc caactcgaca
     6781 tgtgcgaacc cgctggcaac ggtgaaggcg agttcctgaa cggcagtcga accggcctct
     6841 cggatgtggt agccggagat cgacagcggg tggtatttcg ttacgtcggt cgagcagtac
     6901 tccatcatgt ccgccgtcag gcgcagatgc ggttccggcg ggaacagcca ttccttctgg
     6961 gcgatgtact ccttgaggat gtcggtctgg cacgtaccgt cgagcttcga gatgtcggcg
     7021 ccttgtttct ctgccgctgc gatgtacatc gcgaacaaca tgggggccgg ccccgagatc
     7081 gtcatcgagg tagtcagagc ctcgaggtcg agatcggcaa acaggatctc catgtcggcg
     7141 agcgagtcga tggcgacacc acatcgcccg acctcgcctt cggaataagg gtcgtccgaa
     7201 tcccggccca tcagagtcgg catgtcgaaa gcaaccgaga ggcccgtctg gccgtgctcg
     7261 acaaggaact tgaagcgttc gttggtttgt tgggggctgc cgaaaccggc gaactgacgc
     7321 atggtccaaa gccggccccg gtacatcgtc gggtagatgc cgcgagtgaa cgggtactcg
     7381 ccgggtacgc ctaccctcgg atcgacccct tcctcggggc catacacggg gtccagctct
     7441 accgaggaaa gcgtgtcgaa atcgatgtca tcgcgcagct tcgactgctc gtatccctcg
     7501 cgccagcgtc gttgctgatc gtcggccatg tgctcagttt aaggggccgg ttcaagcgtt
     7561 cttttcgaaa ctgtgccgcc tggttagggg gccggatgtg cagaaagaac ggttaggagg
     7621 cgctcggccg cggcgtaggg atccagctcg cgggcggcca ctttgtcggt catctcctcc
     7681 agcagagaag cagcttccgc ttcgagccgg gcgcggtaac gctccgccac gatctctttg
     7741 agctctcgcc ggatc
//
LOCUS       DATMED010000053         5841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5028186, whole genome shotgun sequence.
ACCESSION   DATMED010000053 DATMED010000000
VERSION     DATMED010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5028186"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VNP73_01320"
     CDS             complement(<1..699)
                     /locus_tag="VNP73_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HWL64587.1"
                     /translation="MRRTIGTLAVVALLASAIEAGAVEQWRPEPGVTWQIQLQGRPDL
                     SIDAQVYELDGFDVSEEKVAALHDAGRRVICYMNGGAWEDWRPDADDFPEEVKGKELE
                     GWPGERWLDVGRLDLLQPIMEARLDICVEKGFDAVDFDNVDGYTQDSGFDISWDDQIA
                     YNRWLAQAAEDRGLAAGLKNDLEQVPELVGDYEFSVNEQCFLYRECKLLKPFIDANKP
                     VLNIEYELARRRFCP"
     gene            750..911
                     /locus_tag="VNP73_01325"
     CDS             750..911
                     /locus_tag="VNP73_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64588.1"
                     /translation="MTRKKDDAEEEHVLSLDELVDEASMESFPASDPPSYWGRSSEDE
                     RSETSDEGD"
     gene            complement(894..1622)
                     /locus_tag="VNP73_01330"
     CDS             complement(894..1622)
                     /locus_tag="VNP73_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL64589.1"
                     /translation="MTEEVTPGVFRLGNRTVNWWVVATSDGLTVVDAGLRRHFAQLER
                     LLEHLGRSLTDVEAVVLTHADVDHIGVAEDLRRHGVPVFIHEFDSPGARGKMRALPKE
                     ALLSFWRPQAMVATFEYARDGALRPRFVKETSAISDGDILPVPGSPRVLHVPGHSRGS
                     CALFLEERSILFTGDALVTGDPLSGKREPTLLPKYDNVDHEQALRSLDTLSATGADLV
                     LPGHGLPWEAGVAAAASLASVAFV"
     gene            complement(1626..2006)
                     /locus_tag="VNP73_01335"
     CDS             complement(1626..2006)
                     /locus_tag="VNP73_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL64590.1"
                     /translation="MFKDAKAFSSFSVNDISKAKDFYGGTLGLEVKEGDMGELELNLA
                     TGATVLIYPKENHQPATFTILNLPADNVDETVDKLTAAGVRFENYDFGEIKTDEKGIM
                     RGNGPTIAWFTDPAGNIISVIESG"
     gene            complement(2017..2760)
                     /locus_tag="VNP73_01340"
     CDS             complement(2017..2760)
                     /locus_tag="VNP73_01340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL64591.1"
                     /translation="MTPKADLPRMYGEFAHWFHLLTPPHEYAVEADFYARVLTESAEI
                     PIGTILELGSGGGNNASHLKERFELTLVDLSEEMLELSRVLNPELEHIQGDMRTLRLG
                     RRFDAVFVHDAVCYMTTEVDLMAAIETTFEHCRPGGAAVFAPDWATESFEESTDHGGS
                     DGDGLGMRYLEWVWDPDPGDTTYIADYAYLLRDREGSVRAVHDRHICGIFPRSTWLRL
                     IEGAGFRASRRPGIEDETGMDIFVGVKPV"
     gene            2793..4217
                     /locus_tag="VNP73_01345"
     CDS             2793..4217
                     /locus_tag="VNP73_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWL64592.1"
                     /translation="MTVAGNLGELKASGYPDRSVKDELRTNLLARLSSGDKLFPSIVG
                     YDDSVIPSIERAVLAGHDVIVLGERGQAKSRIMRYLAGLLDEHVPAIAGCEVNDHPYH
                     PICARCRELVAREEDATPIEWVHRDLRYSEKLATPDVSVADLIGDVDPIRVAEGRYLG
                     DELTIHYGLVPRTNRGILALNELPDLPERIQVSLLNILEERDIQIRGYKIRLPLDLML
                     IASANPEDYTHRGRIITPLKDRFGTQVRTHYPQRVEDEVAIMEQEARPAPEGIPIYIP
                     GYLKEVVAAFAALLRTSPQVNQRSGVSVRFSIGALETIVASALRRAVINNEPEAVPRP
                     SDLQSIVHSSIGRIEFDTLEEGREHEILERALKEAVLEIWQRHATGEDLAPVLDAFDE
                     GLSVEVSGDMGSDEFLRQLPVDDREVAAAFERVQQRLKLPEVTPAARASALEFCLEAL
                     HLNRRLNKTQARETGRVRFAAPSV"
     gene            4214..>5841
                     /locus_tag="VNP73_01350"
     CDS             4214..>5841
                     /locus_tag="VNP73_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64593.1"
                     /translation="MKARFSRWDGTQDPFGTEVDVRAVLEQMSDELLSGYGLDWSLRE
                     MQRRGMSGRTGLDDLMRRLREQRQRMADQTNLAGPLAEVKRELDEILSLERAALSKER
                     SDTARMKEMTLDALPNHPAGALRELGTYEFASGEAKARFDALLEKLKRDVLNSYFKNL
                     AGAMRDITPEDIARIKDMLAELNEMIAARNRGDDYDFEGFMSRYGDFFPENPKDLDEL
                     LEVMARRMAAMSRLMASLTPEQRRELAELANTLLDDMDLAWQMDQLGQSLRELMPELP
                     WDEASQMYGEDFMPMSAAVDAIERISEMEELEETLNLDYPGARLEDIDEEKLRRSLDE
                     DAVQDLRWLKEVEQALEKAGLVKRIGGRLELTPRGAKLIGERSLTRVMERIRREPTHR
                     AKGGQAEPTGQTRAWKFGDSDPISVERTVYNAVLRSGPGRDVKLKPEDFEVVETESRP
                     RTATALLLDLSLSMPLRGHWIYAKRMALALQALIDSKYPQDALYLIGFSDFARRMEAV
                     DLATVGWEHVQGTNMEHAFMLARRALAEDRRAIK"
BASE COUNT         1141 a         1846 c         1813 g         1041 t
ORIGIN      
        1 agggcagaac cggcgacgcg ccagttcgta ttcgatgttg aggaccggct tgttcgcatc
       61 gatgaacggc ttgagcagct tgcattcgcg gtagaggaaa cattgttcgt tgacggagaa
      121 ctcgtagtcc cccaccagct cgggcacttg ctcgagatcg ttcttcaatc ccgcggccag
      181 accgcgatct tccgcagctt gcgccagcca ccggttgtac gcgatctggt cgtcccacga
      241 gatgtcgaag ccgctgtcct gcgtatagcc gtcgacgttg tcgaagtcga ccgcgtcgaa
      301 acctttctcc acgcagatgt ccagtcgagc ctccatgatc ggctgcagta ggtccaggcg
      361 tccgacatcc agccatcgct cgccgggcca accctccagc tccttgccct ttacttcctc
      421 gggaaagtca tcggcgtccg gccgccagtc ttcccacgct ccgccgttca tgtagcagat
      481 cacccgtcta ccggcatcgt gtagcgcggc gaccttctcc tcgctcacat cgaacccgtc
      541 cagttcgtag acttgtgcgt cgatgctgag gtcgggacgg ccttgcaact ggatctgcca
      601 ggtcacgccg ggctcgggcc tccactgctc gactgccccc gcctcgatcg ccgacgccag
      661 cagagccacg accgccaagg ttcctatggt gcgcctcatc tcttcagaat ctagtcgggc
      721 cgcctccaga ccgccatacc ctttgattga tgacccgcaa aaaggacgac gcagaagagg
      781 agcacgtgct gtcgctcgac gaactcgtcg acgaagccag tatggagtct ttccccgcca
      841 gcgatccccc ttcgtactgg ggccgatcct ccgaggacga acgctcggaa acgtcagacg
      901 aaggcgactg aggcgaggct cgcggccgcg gcaacgccgg cttcccatgg cagaccgtgg
      961 ccgggaagga cgaggtccgc cccggtggcc gagagtgtgt ccagagaacg gagcgcctgc
     1021 tcgtgatcga cgttgtcgta cttcggcagc agggtgggct ccctcttccc cgaaagagga
     1081 tcccccgtta cgagcgcgtc accggtgaac aggatcgaac gttcttcgag gaacaacgcg
     1141 caactgcccc ggctgtgtcc cggtacgtgc agaacgcgtg ggctaccggg cacgggaaga
     1201 atgtcaccat cggagatcgc gctggtttcc ttcacgaaac ggggtctaag cgcaccatcg
     1261 cgcgcgtact cgaacgtcgc gaccatcgcc tggggccgcc agaagctgag tagagcctcc
     1321 tttggtagcg ctcgcatctt gccgcgcgcc cccggcgagt cgaactcgtg gatgaatacc
     1381 ggcacaccgt ggcgacgtag atcttcggca acgccgatgt gatctacgtc ggcgtgcgtc
     1441 aagacgacgg cttccacatc ggttagcgat cgtccaagat gttcaaggag ccgttcgagc
     1501 tgagcgaagt ggcgcctaag tccggcgtcg acgaccgtca ggccgtcaga ggtggcaacg
     1561 acccaccagt tgaccgtccg gttccccaaa cggaaaacgc cgggggtgac ctcttcggtc
     1621 aagcgctagc cgctctcgat gacggagatg atgttgccgg cgggatccgt gaaccacgcg
     1681 atcgtcgggc cgttcccgcg catgatccct ttctcgtcgg tcttaatctc accgaagtcg
     1741 taattctcga aacgaacgcc tgcagcggtc agtttgtcga cggtctcatc gacgttgtcc
     1801 gcaggaaggt tcaggatcgt gaaggtcgcc ggctggtggt tctccttcgg atagatgaga
     1861 accgttgccc cggtggcgag attcagctcg agctccccca tgtcgccctc tttcacctcg
     1921 agtcccagtg tccctccgta gaagtccttc gctttcgaga tgtcgttgac ggagaaactt
     1981 gagaaagcct tcgcgtcctt gaacattcga tcctccttag acgggtttca ctcccacgaa
     2041 gatatccatg ccggtctcgt cttcgatccc gggacgcctg gacgcccgga atcctgcacc
     2101 ctcgatcagc cgcagccacg tggaccgagg gaatatcccg cagatatggc ggtcgtgcac
     2161 ggccctcacg ctgccctctc gatcccgaag gaggtacgcg taatccgcga tgtaggtggt
     2221 gtcgccgggg tccgggtccc acacccactc gaggtagcgc atcccgaggc catccccgtc
     2281 acttcctccg tgatccgtcg actcctcgaa cgattccgtg gcccagtccg gcgcaaagac
     2341 ggccgcaccg ccgggacggc agtgttcgaa agtcgtctcg atcgcggcca tcagatcgac
     2401 ttccgtggtc atgtagcaga cggcgtcgtg aacgaacacc gcgtcgaacc gtcgccccag
     2461 tcgcagggtg cgcatgtcgc cctggatgtg ctcgagctcg gggttgagca ctcggctcag
     2521 ctcgagcatc tcttccgaca gatcgaccag cgtcagctcg aaccgttcct tcagatgtga
     2581 agcgttgttc ccacccccgc tgcccagctc aaggatcgtc ccgatcggga tctcggccga
     2641 ctcggtcaga acgcgcgcat agaaatccgc ctccaccgcg tattcgtggg gaggagtcag
     2701 cagatggaac caatgggcga actccccgta catcctcgga agatcggcct tgggggtcac
     2761 tagagcgacg cagtctataa gctgtatcgc tcatgaccgt agcgggaaat ctcggtgagt
     2821 tgaaggcaag tggttacccc gatcgttccg tcaaggacga actgcggacc aatctgctgg
     2881 cccggttgag ttccggggac aagctcttcc ccagcatcgt cggttacgac gactcggtca
     2941 tcccctcgat cgagcgggcc gtactcgccg ggcacgacgt gatcgtgttg ggtgagcgtg
     3001 gtcaggcgaa gagccgcatc atgcgttacc tcgcagggtt gctggacgag catgtgccgg
     3061 cgatcgccgg atgcgaggtg aacgaccatc cctaccatcc gatctgcgcg cgctgtcggg
     3121 agctggtcgc gcgtgaggaa gacgcgaccc cgatcgaatg ggttcatcga gaccttcggt
     3181 attccgaaaa actcgcgacc cccgacgtat ctgtagccga cctgatcgga gatgtcgacc
     3241 cgatccgcgt cgccgagggt cgctacctcg gagacgaact gaccatccat tacggactgg
     3301 ttccccgcac gaaccgcggg atcctcgctt tgaacgagct cccggacctc ccggagcgca
     3361 tccaggtttc gttactgaac atcctcgagg aaagggacat ccagatacgg ggctacaaga
     3421 tccgtttgcc actcgacctg atgctgatcg cgtcggcgaa ccccgaggac tacacgcacc
     3481 gcggccggat catcacgccg ctcaaagacc gcttcggcac gcaggtgcgg acgcactatc
     3541 cgcaacgcgt cgaagacgag gttgcgatca tggaacagga ggcgaggccg gctcccgaag
     3601 gcatcccgat ctatatcccc ggctacctca aggaggtcgt agccgccttc gccgctttgc
     3661 tccgcacgag cccacaggtg aatcagcgca gcggtgtctc cgttcgtttc tcgataggtg
     3721 cgctggagac gatcgtcgcg tcggcccttc gaagagcggt catcaacaac gaacccgaag
     3781 ccgtgccgcg gccttccgat ctgcaatcga tcgtgcacag ttcgatcggg cggatcgagt
     3841 tcgacaccct cgaagagggc cgcgagcacg agatcctcga gcgggcactg aaggaagcgg
     3901 tgctcgagat ctggcaacgt cacgcgacgg gagaggacct cgccccggtg ctcgacgcgt
     3961 tcgacgaggg gctatcggtc gaggtctccg gagacatggg ctcggacgaa ttccttcgac
     4021 agttgccggt tgacgaccgc gaggtggctg ctgccttcga gcgcgtgcag cagcgcctca
     4081 agcttcccga ggtcacaccg gctgccagag cgtccgcgct cgagttctgc ctcgaagcgc
     4141 tgcacctgaa tcgacgactc aacaagaccc aagcgaggga gacgggccgc gttcgcttcg
     4201 cggcaccaag cgtgtgaaag cccgcttctc ccgttgggat gggacgcagg atccattcgg
     4261 caccgaggtc gacgtgcgag cggtcctgga gcagatgtcc gacgagctcc tgtccggcta
     4321 cgggctcgac tggtcgctcc gagagatgca gcgcagaggg atgtcggggc gaaccggctt
     4381 ggacgacctc atgcgcagat tgcgggaaca gcgccagagg atggccgacc agacgaacct
     4441 ggccggcccc ctcgcagagg tcaaacgcga gctcgacgag atcctctcgc tggaacgcgc
     4501 cgccctatcg aaagagcgct ccgacacagc tcgcatgaag gagatgactc tcgacgcctt
     4561 gccgaaccac cccgcaggtg cactgcggga actcgggacg tacgagttcg cgtcgggtga
     4621 ggccaaggcg aggttcgacg cgttgctcga gaagctgaaa cgagacgtcc tcaactcgta
     4681 cttcaagaac ctcgccgggg cgatgaggga cataactcca gaggacatcg cccgcataaa
     4741 ggacatgctg gcggagctga acgagatgat cgcggcgcgc aaccgcggcg acgattacga
     4801 cttcgagggt ttcatgtccc gttacggaga cttcttcccc gagaacccga aggatctcga
     4861 cgagttactc gaggtgatgg cccggcgcat ggcggcgatg agccggctga tggcaagcct
     4921 cacccccgaa caacgacgtg agttggccga gcttgctaac acgctgctcg acgacatgga
     4981 cctcgcgtgg cagatggacc agttgggaca gagcctgcgc gagctgatgc ccgagctccc
     5041 gtgggacgaa gcctcgcaga tgtacggcga ggacttcatg ccgatgtctg cggccgtcga
     5101 cgcgatcgag cggatcagcg agatggagga gctcgaggaa accttgaacc tggactatcc
     5161 cggtgcccgg ctcgaagaca tcgacgaaga gaagttacgt cgctcgctcg acgaggacgc
     5221 ggttcaggac ctgcggtggt tgaaagaggt cgagcaagcg ttggagaagg cgggactggt
     5281 gaaacggatc ggcgggcgat tggagctgac gccgcgcggt gcgaagctga tcggcgaacg
     5341 atcgctcacg cgcgtgatgg agcggatccg ccgggagccg acgcatcgcg caaagggagg
     5401 ccaagccgag cccacaggcc agacgagagc gtggaagttc ggagactcgg atccgatctc
     5461 ggtcgaacga acggtctaca acgcggtcct acgctcggga ccgggccgcg acgtcaagtt
     5521 gaagcccgag gatttcgaag tcgtcgaaac cgaatccaga ccgcgtacgg caacggcgct
     5581 tctcctcgac ctttcgttgt ccatgccctt gcggggacac tggatctacg cgaagcggat
     5641 ggcgctcgcg ttgcaagcgc tgatcgactc gaaatatccg caggacgcgc tgtacctgat
     5701 cgggttctcc gacttcgcac gacggatgga agccgtcgat ctcgccacgg tcggatggga
     5761 acacgtgcag ggaacgaaca tggagcacgc gttcatgctc gcccggcgcg cattggccga
     5821 ggaccgcagg gcgatcaagc a
//
LOCUS       DATMED010000054         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1962650, whole genome shotgun sequence.
ACCESSION   DATMED010000054 DATMED010000000
VERSION     DATMED010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962650"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="VNP73_01355"
     CDS             <1..164
                     /locus_tag="VNP73_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese ABC transporter ATP-binding protein"
                     /protein_id="HWL64594.1"
                     /translation="TVTEYFDSVTLMNVRRIASGATNDVFTEENLRATYGGRMAWLKT
                     ASQTEPAQD"
     gene            164..1066
                     /locus_tag="VNP73_01360"
     CDS             164..1066
                     /locus_tag="VNP73_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWL64595.1"
                     /translation="MFDLFTDYTLRTVALGAGILGIVSGVLGSFTVLRHQSLLGDAIS
                     HAALPGIVLAFLLTGSNEPLPLIVGAGVAGWLGTLLVRAIVRNTRIKNDGALGLVLSV
                     FFGMGLMLLTYSQSRPDASQAGLDKFLFGQAAALLRNDVITIAILGGVAIVVVALFWK
                     EFKLLSFDPEFGSVLGFRMGTIDVLLTTLVVIAIVIGLQTVGVVLMSAMVVAPGAAAR
                     QWTDRLGVMVCLSALFGALSGVTGALLSSTGVRIPTGPMVVLSASAIVLLSLLFAPNR
                     GLVWDKLRSRRVAISADPREQAGL"
     gene            1083..2192
                     /locus_tag="VNP73_01365"
     CDS             1083..2192
                     /locus_tag="VNP73_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWL64596.1"
                     /translation="MSEAQLEIQAIALVTAVACALPGAFLVLRRMALMTDAISHSILL
                     GIVLAFFVVGSVGSPLLIVGAALMGIVTVVLVETLANTKRVGKDAAIGLVFPALFSIA
                     VILIARFADDVHLDVDAVLLGELAFAPFERLAINGYDLGPRALWVMGVILVVNLAGLA
                     LFYKELKVATFDSALAAALGFSPVLVHYGLMGAVSVTAVGAFDAVGSVLVVALMIAPP
                     ATAYLLTDRLSRMIATSALIGAVTAIGGFWLAHWLDASIAGSMATTAGFIFGLAFLFA
                     RDRGLVALLRRRKRQRWEFAQEMLVVHLLHHEHGPDAEQENRVAHLQEGLRWEGEFTE
                     EVVGRARRRGLVSLADGLLTLTDAGRDRARRSFAA"
     gene            2202..>2438
                     /locus_tag="VNP73_01370"
     CDS             2202..>2438
                     /locus_tag="VNP73_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64597.1"
                     /translation="MMEHPIRNESPPLEARLPVVVDGLRLRGGAYVPAEAQGLVLLLH
                     GIPSTAPPDPEDTGYQGFARHLAARGFAAAWVDMR"
BASE COUNT          367 a          769 c          784 g          518 t
ORIGIN      
        1 agaccgtgac tgagtacttc gactccgtga ctctgatgaa cgtgcgacgc atcgcgagcg
       61 gagctaccaa cgacgtcttc acggaggaga acctgcgggc gacgtacggg ggccgcatgg
      121 cctggctgaa gaccgcatcg cagacggaac ccgcgcaaga ctgatgttcg atctcttcac
      181 cgactacacg cttcgaacag tggctttggg ggccgggatt ctcggcatcg tcagcggggt
      241 gctcggatcg ttcactgtgt tgcggcacca gagtcttctc ggagacgcga tctcacacgc
      301 ggcgcttccc ggcatcgtcc tcgccttcct gctgaccggc agcaacgagc ccctgccgct
      361 gattgtcggc gccggggtcg ctggctggtt gggcacgttg ttggtccggg cgatcgttcg
      421 caataccagg atcaagaacg acggtgccct gggtttggtg ctctcggtgt tcttcgggat
      481 gggattgatg ctgttgacct actcacagtc gagacccgac gcgagtcaag ccggcctcga
      541 caagtttctc ttcggccagg cggcagctct actccgcaac gacgtcatca cgatcgcaat
      601 cctgggtggc gtggcgatcg tcgtcgtggc gctcttctgg aaggagttca agctgctgag
      661 cttcgatccc gagttcggga gcgtactcgg gttccgaatg ggaacgatcg acgttctcct
      721 cacgaccctc gttgtcatcg cgatagtcat cgggctccag accgtcggcg tcgtcctgat
      781 gagcgccatg gtggttgcac ccggcgcagc cgcgcgccag tggaccgacc ggctcggcgt
      841 gatggtctgt ctgagcgcct tgttcggcgc cctgagtggg gtgaccggcg ctctgctcag
      901 tagcacgggg gtccgaatcc ccaccggacc catggtcgtc ctctccgcga gtgcgatcgt
      961 cctgctgtcg ttactgttcg ctcccaaccg cggcctggtg tgggacaagt tgcgctcacg
     1021 tcgcgtcgcg atctcggccg atccgcgaga gcaagccgga ttgtgatggt cgaggtgaag
     1081 tcgtgagcga ggcacaactc gagatccagg ccatcgctct tgtaacggcc gtcgcgtgcg
     1141 cgcttccggg agctttcctg gtccttcgtc gaatggcgct gatgaccgac gcaatcagcc
     1201 attccatcct gcttgggatc gtcctcgcct tcttcgtcgt tggatccgtg ggctcgccac
     1261 tgctgatcgt gggggcagcg ttgatgggaa tcgtcacggt cgtactggtt gagacgctcg
     1321 cgaatacgaa gcgcgtcggc aaggatgcag caatcggcct tgtattcccg gcactcttct
     1381 cgatcgccgt gatcctgatc gcgcgtttcg ccgacgacgt gcacctcgat gtcgacgcgg
     1441 tgttgctcgg cgagctcgct tttgcgccct tcgagcggtt ggccatcaac ggttacgacc
     1501 tcggcccccg cgccctgtgg gtcatgggcg tgatcctcgt agtgaacctg gcgggcctcg
     1561 ccctcttcta caaagagctc aaggttgcga ccttcgactc tgctctagcg gcggccctcg
     1621 gcttctctcc ggtcctcgtg cactacgggc tgatgggcgc ggtgtccgtt acggcggtcg
     1681 gtgccttcga tgcggtcggt tccgtgctgg tagtagcact gatgattgct ccgcccgcca
     1741 ccgcgtacct cctcaccgac cgcctatcgc gcatgatcgc gacgagtgcc ctgattggtg
     1801 ctgtcaccgc aatcggcggc ttctggttgg cccattggct cgacgcatca attgcggggt
     1861 cgatggcaac cacggcgggg ttcatcttcg gtcttgcttt cctgttcgct cgcgaccggg
     1921 ggttggtagc tctccttcgc aggagaaagc gacagagatg ggaattcgcg caggagatgc
     1981 tcgtcgttca cttgctccac cacgagcatg gacccgatgc cgaacaggag aatcgagtgg
     2041 ctcaccttca ggagggcctt cgctgggagg gtgagttcac cgaggaagtg gtcggccgcg
     2101 cacgccgcag gggcctcgta tctctagccg atggcctgct caccctgacg gacgccgggc
     2161 gagaccgcgc gcgccgatcc ttcgcggcct gattcaatca gatgatggaa cacccaatca
     2221 ggaacgagtc gcctcccctg gaggcgcgat tgccggtcgt cgtcgacggg ctgcggttgc
     2281 gaggaggcgc ctacgttccc gctgaggcgc agggtctcgt cctccttctt cacggcatcc
     2341 cgtcaaccgc tccgcccgat cccgaggaca cgggttacca ggggttcgcc aggcaccttg
     2401 cggcccgggg cttcgccgct gcctgggtcg acatgcgg
//
LOCUS       DATMED010000055         6392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_43446, whole genome shotgun sequence.
ACCESSION   DATMED010000055 DATMED010000000
VERSION     DATMED010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43446"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VNP73_01375"
     CDS             complement(<1..824)
                     /locus_tag="VNP73_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64598.1"
                     /translation="MKPKGDRRRLVYVLLVAILVGTVVIPLSAARAACGEQNGQWVSV
                     SGPNFGDGPQAIVAFAIDSRNPRRMFASNGPTVMRSQDGGCSWRESLVVAEETSFQRP
                     VGEPPRVKSIAIPEGNGARVYLLLEEQVGPATRPHVMVSANGGNSWTVSDTALPPLGT
                     PGLLRAAPSNPAVAYLTVDVAGASDALFATQDGGGSWQLRSRVPTGGLIDMKIDPLDA
                     QNIWLGGATGLFHSTDGGLQFEAIPQFADAPAGPIDVFHGPKGPARLFVFSSAGPS"
     gene            892..1176
                     /locus_tag="VNP73_01380"
     CDS             892..1176
                     /locus_tag="VNP73_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64599.1"
                     /translation="MSEERRSGGGANPSGAEQGPDKRKEPVVVDLERLEKHAEQIYLH
                     RFVAYIRKLRNEHGRYLTLRAGDETAILAAEDGSAESLKEVLKDSDETDT"
     gene            complement(1184..3490)
                     /gene="ftsH"
                     /locus_tag="VNP73_01385"
     CDS             complement(1184..3490)
                     /gene="ftsH"
                     /locus_tag="VNP73_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWL64600.1"
                     /translation="MSNEPDSGNPPPPPPPPPGSKTAPGGITAASAARKGGLVANVRE
                     AAERYMLRRRRARAAKQKMATRKTTIALSLVVIILMGAFAWSLNYNSPRSEGREVSFD
                     VLAALAAENRVVTATFRDEDNRIVGTYAAQSILPEPEPEPKNKGKNAKGNAKNDKGAN
                     RPGGDDKNAKNDNKPKPKPEPEAEEVLNAPPGSGEYWLSYPSSDAAFGVLSELLTAAG
                     SEVRVDPQTTKFIVRSVSTYLLPLLILAAFFGLLFTGARGGGSAIGEVMSFGTSGAKR
                     QKRGFTQPVTFNDVGGEQEAVVELKEVVDYLQNPKRYEEIGAVPPKGVLLFGPPGCGK
                     TLLAKAVAGEAGVPFFSVSGAEFVESLVGIGAARVRDLFRRVRAVAPAIVFIDELDAT
                     GRRRGGGEGGGSDEREQTLNQLLVEMDGFEISGGIVVVGATNRPDILDPALLRPGRFD
                     RHITVDQPDAKGRKEILDIHSRNKPLASSVDLTYIAKRTPGFSGADLANVINEAALLS
                     IRDEKGEIETVELEEAIQRVLHGPKRRGRVLSPEEERRGAYHEAAHAVVAASVGRGDE
                     VHRVTILARAKGLASIGTLGLERSEADNLFFTRDQLFSQIVIAMAGLACEEVVLGDMS
                     TGAEEDIEKATEVARDIVGRYGMSPVLGRTRILASDVDKFLGGDAGFGQISPETHEQF
                     DNEVRRLVIAGETEARRIIDVNRPALDRLAAQLEELETLEGRVLVELLQPISVDKAGL
                     SRPFTEFEGNGLGYPVRSRPAGKSSPSS"
     gene            complement(3678..4265)
                     /gene="mug"
                     /locus_tag="VNP73_01390"
     CDS             complement(3678..4265)
                     /gene="mug"
                     /locus_tag="VNP73_01390"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HWL64601.1"
                     /translation="MSRPITTRGATRPERPTKDELEAARDKKISDVMAPGLEVLFCGI
                     NPGLYSGATGHHFARPGNRFWPALHKSGFTPRLLSPFEERELLELGLGITNVVNRTTA
                     TAAELSTDEIKAGAAALRRKVKRSAPRVVAVLGVQTFRVGWGLPRAEIGPQEGSIGGT
                     RIWVLPNPSGLNAHYQLPELARLFARLRTASRQRA"
     gene            4264..4713
                     /locus_tag="VNP73_01395"
     CDS             4264..4713
                     /locus_tag="VNP73_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64602.1"
                     /translation="MRWKVTVIALALIAGACGSGGPPPIGIPTCSPVDKPAIDPVEGL
                     VLPPGATLYRSKPTGERLILVRGFIEMTPVRIREFYERLQTKPRYRFFLLEDEVVEAE
                     AFFTDGRWRNFVRARIACEGRTELYVLVAPEDYGNRIDGRPSTASPK"
     gene            complement(4767..6230)
                     /locus_tag="VNP73_01400"
     CDS             complement(4767..6230)
                     /locus_tag="VNP73_01400"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL64603.1"
                     /translation="MAGRIRNPKVWFVLVLAAIAVGGMTVAPASHQDSAKPVAGKHQA
                     YHGKIYDKKAPGKLYPANKSVAYSGPKLEVQAVPIGREAAEPTVAVLKDGSAFFAAGT
                     FDQAAGNLARTEIYRSTDGGVKWESVQPEIAPGQHESPQTLDPYVYADPVGGRVFSVD
                     LYTVTGSYLLWSDDQGETWSRNPLASAGWSVDHQTFYSGPPPEGVETNGYPSVLYYCT
                     NRVADATCARSMDGGVVWQPTGEPAFLGEDEEAGGFCGGLHGHAAVDTKGRLFLPKGH
                     CDRPWVGMSEDMGASWTRVQASKWPRTAGPHIEIATDAADNVYLVWWSERRRLPYLVI
                     SRDHGATWSHPRMIAPPGVRQTNFPVIAAGDAGRIAILFPGSTVKSQTDATRPWHDYV
                     VMSTNALSKNPLFVSTTTSPLSDPVHRGNCSGRCGGMFDFLDLFVSPHDGSLWGATAD
                     NCVELCATEGTGPLKQVGDGVAVKQLSGPSLWAPGHK"
BASE COUNT         1097 a         2139 c         2035 g         1121 t
ORIGIN      
        1 tgactcggtc ccgccgaaga gaagacgaat agtcgggcgg gccctttcgg accgtggaac
       61 acgtcgatcg gtcctgccgg ggcgtcggcg aactgcggga tcgcttcgaa ctggaggcct
      121 ccgtcggtcg aatggaaaag ccccgttgca ccgccgagcc agatgttctg agcgtccagc
      181 gggtcgatct tcatgtcgat caagccgccc gtcggaacgc gggaccgaag ctgccacgac
      241 ccacccccgt cctgtgtcgc gaacagggca tccgacgccc ccgctacatc gacggtgaga
      301 tacgcgaccg ccgggttgct gggagcggct ctcaatagac cgggggtgcc gagggggggc
      361 agagccgtat ccgacaccgt ccagctattg ccaccgttag cgctcaccat cacgtggggt
      421 ctggtagcgg gtccgacctg ctcttcgaga aggagataga cgcgagctcc gttgccttcg
      481 gggatcgcga tgctcttcac acgggggggc tcgccgacgg gtcgctgaaa cgaggtctcc
      541 tcggccacga cgagggattc acgccacgag caaccaccgt cctgtgaccg catcacggtc
      601 ggcccattgg aggcaaacat ccgccgaggg ttgcgtgagt cgatcgcgaa ggcaacgatc
      661 gcttgagggc cgtctccgaa gttcgggccg gacaccgaga cccactggcc gttctgctcg
      721 ccacaagccg ctcgggccgc gctcaacggg atcacgaccg tcccgaccag gatcgcgacc
      781 aacagcacgt aaacgaggcg gcgcctgtcc cccttaggtt tcatcgatat cttcaacctt
      841 ctccggggat gatcccgtcc ctaccttggt ccaccccgta atcttccccc catgagcgag
      901 gaacgacgct ccgggggtgg ggccaacccc agcggagccg agcaaggacc cgacaagcgc
      961 aaagagccgg tcgtggtcga cctggagcgg ctcgagaagc atgccgagca gatctacctg
     1021 caccgcttcg ttgcctacat ccgcaagctg aggaacgagc acggccgtta cctcaccctt
     1081 cgggcaggag acgagacggc gatcctggcg gccgaggacg gaagtgccga gtcgctgaag
     1141 gaagtcctca aagactcgga cgagaccgac acctaacgga cctctagctc gaaggcgaag
     1201 acttgccggc cggacgagag cgaaccgggt atccgaggcc gttgccttcg aactcggtga
     1261 aaggtcgcga cagtccggcc ttgtcgaccg agatcggctg aagcagttcc accagaacgc
     1321 ggccctcgag agtctcgagc tcttcaagtt gagctgcgag ccggtcgagc gcgggacggt
     1381 tgacgtcgat gatccgtctg gcttcggtct cccccgcgat cacgagcctg cgcacctcgt
     1441 tgtcgaactg ctcgtgcgtc tcgggcgaga tctgtccgaa accggcgtcg cctccgagga
     1501 acttgtccac gtcggaagcg aggatgcgcg tccgaccgag aacggggctc atcccgtagc
     1561 ggccgacgat gtcgcgagcg acctcggtcg ccttctctat gtcctcctcg gcccccgtcg
     1621 acatgtctcc gagcaccacc tcttcgcacg ccagacccgc catcgcgatc actatctgcg
     1681 agaacagctg gtcgcgggtg aagaagagat tgtctgcttc ggagcgttcg agccccaacg
     1741 ttccgatcga ggccagccct ttcgcccggg cgaggatcgt cacgcggtgc acctcgtcgc
     1801 cgcgacccac cgacgcggcc accacagcat gagcggcctc gtggtaggcg ccccggcgtt
     1861 cctcttccgg tgacagcacc cggccccggc gcttcggtcc atgcagcact cgttggatcg
     1921 cctcttcgag ctcgacggtt tcgatctcgc ccttctcgtc ccggatcgac agcagagccg
     1981 cttcgttgat cacgttcgcg aggtcggcgc cggagaaccc tggcgtcctt ttggcgatgt
     2041 aggtgaggtc gaccgacgat gcgagcggct tgttacgcga atggatatcg aggatctcct
     2101 tgcggccctt cgcgtcgggc tggtcgacgg tgatgtgccg gtcgaaacga ccgggacgga
     2161 gcagcgccgg gtcgaggatg tcgggtcggt tcgttgcacc aaccaccacg atcccgcccg
     2221 agatctcgaa accgtccatc tcgacgagta gctggttcag agtctgctcg cgctcgtcgg
     2281 aaccgccacc ctccccgcca cccctgcgac ggccggtggc gtccaactcg tcgatgaaga
     2341 cgatcgccgg agcaaccgca cgaacccggc ggaagaggtc acgcacgcga gccgcaccga
     2401 tgccgaccag tgactcgacg aactcggcgc ccgaaacgga gaagaaggga accccggcct
     2461 cgccggcgac cgccttggcg agaagcgttt tcccacaacc gggggggccg aacaacaaga
     2521 cacccttggg tgggaccgcg ccgatctcct cgtagcgctt cgggttctgc aggtagtcga
     2581 cgacctcttt gagctcgacc acggcttctt gctcgccccc gacatcgttg aaggtgacgg
     2641 gctgcgtgaa gcctctcttc tgacgcttcg ccccggaggt accgaacgac atgacctcgc
     2701 cgatcgcaga accgccgccg cgcgcgcccg tgaacaacag gccgaagaat gcagccagga
     2761 tcagcaatgg aagcagatag gtggacacgg accgcacgat gaacttggtg gtctgcggat
     2821 cgactcggac ctccgagccg gcggccgtaa ggagctcgga cagaaccccg aacgcagcat
     2881 cgctggacgg gtacgacagc cagtactccc ccgaccccgg cggcgcgttg agaacttctt
     2941 cggcttcggg ctccggcttc ggtttcggct tgttgtcgtt cttcgcgttc ttgtcatcgc
     3001 cgccgggccg gttggcaccc ttgtcgttct tcgcgttgcc cttagcgttc ttgcccttgt
     3061 tcttcggctc cggctcgggc tccggcagga tcgactgcgc cgcgtaggtg cccacgatcc
     3121 ggttgtcctc gtcgcggaac gttgccgtca cgacgcggtt ctcggccgcg agcgcggcca
     3181 gcacgtcgaa cgacacctcg cggccctcgg agcgcggaga gttgtagttg agcgaccagg
     3241 cgaacgcgcc catcaggatg atgaccacga ggctgagggc gatcgtcgtc ttgcgggtcg
     3301 ccatcttctg cttagcggcc cgcgcgcgcc tgcgtctgag catgtagcgc tcggccgcct
     3361 cacgcacgtt cgcgacgagg ccacccttcc ttgctgcgga ggccgcggtt atcccgccgg
     3421 gagcggtctt agagccgggc ggaggtggtg gcggtggagg tggattgcct gaatctggtt
     3481 cgttggacat atttcctctt cgagtgggga ctcttcattg agtcggccgg gcaagactaa
     3541 aggaactacc cccgtagtag ataacggctt gggttctcct actcccttaa tacgtaccag
     3601 ggctcttagc gtcacgccac ggctctccgc aggccaccgc gtcacgccct cgggcgtgag
     3661 gccgtcctca aacgggctca cgccctctgg cgtgacgccg tcctcaaacg ggcgaacagt
     3721 cgggccagct ccggcagctg atagtgagcg ttgagaccgc tggggttggg cagcacccag
     3781 atccgggtgc ccccgatcga cccctcctgg ggtccgatct ctgcccgggg cagaccccag
     3841 cccacccgga aggtctgcac ccccagcacg gccacgaccc ggggggcgct gcgcttcacc
     3901 ttccgcctca aggcggcggc tcccgccttg atctcgtcgg tcgatagctc ggcggcggtc
     3961 gccgtcgtcc ggttgaccac gttcgtgatg ccgaggccga gctcgaggag ttcccgctcc
     4021 tcgaatgggc tcaacagccg gggcgtaaac ccgctcttgt gaagtgccgg ccagaagcgg
     4081 ttgcccgggc gggcgaaatg gtggcccgtg gcgcccgaat agagcccggg gttgatgccg
     4141 cagaacaaga cctcgagtcc gggagccatc acgtcggaga tcttcttgtc gcgggcggcc
     4201 tcgagctcgt ccttcgtagg acgctccggc cgtgttgctc cacgtgttgt gatgggacgc
     4261 gacatgaggt ggaaggtaac cgtgatcgca ctcgcactga tcgcaggagc ttgcggttcc
     4321 ggaggtcccc caccgatcgg gatccccaca tgctctccgg tcgataaacc agcgatagat
     4381 cctgtggagg ggctcgtcct tccccccgga gccaccctct acaggtccaa acccaccggg
     4441 gaacggctga tcctcgtccg agggttcatc gagatgacgc ccgttcggat acgcgagttc
     4501 tacgagcggc tgcagacgaa gccgcgctac aggttcttcc tcctcgagga tgaggtggtc
     4561 gaagcggagg ccttcttcac ggatgggaga tggcgcaact tcgtcagagc tcggatcgcg
     4621 tgcgagggcc gaaccgagct gtacgtgctc gtagcccccg aggactatgg gaacaggatc
     4681 gacggtcgcc cgagtaccgc gtcacctaaa tagcgccgaa cgtcaagacg ttcggcgttc
     4741 gggctagatg ctctgatcta agtaactcac ttgtggccgg gggcccagag cgacggtccc
     4801 gacagttgct tgacggcgac cccatctccg acctgcttca acggccccgt accttcggtg
     4861 gcgcacagct cgacgcagtt gtccgcggtc gccccccaca ggctgccgtc gtgcggcgag
     4921 acgaacaggt cgaggaagtc gaacatgcca ccacaccgac cgctgcagtt cccccggtgt
     4981 acgggatcgc tcagcgggct ggtggttgtc gaaacgaata gtgggttctt actcagcgca
     5041 ttggtcgaca tgacgacgta gtcgtgccac ggacgcgtcg cgtcggtttg actcttcacg
     5101 gtcgaaccgg ggaacaggat cgcgatcctg cccgcgtcgc cggcggcgat caccgggaag
     5161 ttcgtctgcc tcacgcccgg cggagcgatc atccgagggt gcgaccacgt cgccccgtgg
     5221 tctctcgaga tcacgagata gggaagacgc cgtctctccg accaccacac cagatagacg
     5281 ttgtcggccg cgtctgtcgc tatctcgatg tggggccctg ccgttctcgg ccacttcgag
     5341 gcctggacgc gcgtccacga cgcccccatg tcctccgaca tgcccaccca cggacggtcg
     5401 cagtgacctt tgggaaggaa cagccgacct ttggtgtcca ccgctgcgtg gccgtggagg
     5461 ccaccacaga atccgccggc ctcttcgtcc tcacccagga acgcaggctc cccggtgggc
     5521 tgccacacca cgcccccgtc catggaacga gcgcacgtcg cgtcggccac gcggttggtg
     5581 cagtagtaga ggacgctcgg gtacccgttc gtctcgacac cctcgggcgg cggcccggaa
     5641 tagaacgtct ggtggtcgac cgaccatccc gcggaggcaa gtgggttgcg cgaccaggtc
     5701 tcgccctggt cgtcgctcca caacagatag gaacccgtca cggtgtagag gtcgacggag
     5761 aacacgcggc ccccaacggg gtccgcatag acgtacgggt cgagtgtctg aggcgactcg
     5821 tgctgaccgg gggcgatctc gggctgcacc gactcccact tcaccccacc atcggtcgag
     5881 cggtagatct cggtgcgggc gaggtttccc gctgcttggt cgaaagttcc ggcggcgaag
     5941 aacgccgacc cgtccttcag taccgcgacc gtgggttcgg cggcctcgcg accgatcggc
     6001 accgcttgga cctcgagctt cgggcccgag taagcgacgg acttgttggc cgggtaaagc
     6061 ttgcccggcg ccttcttgtc gtagatcttc ccgtgatagg cctggtgttt gccggcgacc
     6121 ggctttgctg agtcctgatg gctcgccggt gccacggtca tgccaccgac cgcgatcgcc
     6181 gccagcacca acacgaacca aacctttgga ttcctgatcc gcccagccat ccgcccaacc
     6241 tccttgtcgg ccccggagac aggataggcg cattcacacc gttaaggtcc cgctcgtgac
     6301 cttcttcgag acgacgactg cagtcgaacc gctgggtgac gggcgttacc gggccgagct
     6361 cgacgacgcc tggtgggtgg ttcgaggccc cc
//
LOCUS       DATMED010000056         3899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1188419, whole genome shotgun sequence.
ACCESSION   DATMED010000056 DATMED010000000
VERSION     DATMED010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188419"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNP73_01405"
     CDS             <1..309
                     /locus_tag="VNP73_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64604.1"
                     /translation="NHRTWVDIAGWLLGALFLIGLWRGRRWAFTLWFVFTSLALVVSA
                     IAYVFIPLPEAARFPIAETVLSLIQLALLMHPATKRFAGTERSPLPQADAPTPAQSDY
                     "
     gene            complement(428..1372)
                     /locus_tag="VNP73_01410"
     CDS             complement(428..1372)
                     /locus_tag="VNP73_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64605.1"
                     /translation="MKRSFVVVGQLLVLLVVVLPTSNASAPPIAAFAGTNHFSSTRSG
                     EAVLHLTESVKVSTDPFGNKLARIRSTGQVAGLMLKQDVPKNGVEIIALSVALCAKPP
                     CKERRLNYTQVWDPSNHGFPRNVTLPSGDYFVRLITDGYPTDVDLTFSGLSGETTQDL
                     SATQDVDVAAPKTTFDPLPEHNVYSAEAKQTLGGMGWAILAVEVEAEGRLYGEIETCL
                     QEKRSDPVRAHLSVGFFFTSCDGSGAGSTAFGLPTGPNREVVVELTSLDAGTYFHSLA
                     YKTVAEVQAVNSIAVNVEFPRGERSGGRSAGVFHSDEG"
     gene            1513..2631
                     /locus_tag="VNP73_01415"
     CDS             1513..2631
                     /locus_tag="VNP73_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWL64606.1"
                     /translation="MQPSTVAQAPQACTWSIGIMGALSGDYKRIGEPMANGIRLAADI
                     ANREGGLPCTLTTRAEDSQGDPNQSPRKARALTEDQQVVACICGFFSGETLASGNVFE
                     RGGLIMVSTGTNRTIKKQGFDTWFRAIAADPRQARATVAYIKENLRARKVVTVHDGQD
                     YSEGLAQDVGDALGSRLRARYRINPEESDYSAVVSKIKRVDPDVVYYGGYTPQAGALL
                     RQLHEARVGARFVVPDGSLDDEFMGRALGRRNVDMEASCPCSDAQKIERAQEFAREYE
                     DAYGEEPGIYSADTFDVANIAIDALGELTGLETTEQVRAHVVEYFDNVEHAAGTVKSY
                     DWSDRGELKAGADHVFVWKWDNGTRKFEMLGRVSELTS"
     gene            2638..3159
                     /locus_tag="VNP73_01420"
     CDS             2638..3159
                     /locus_tag="VNP73_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWL64607.1"
                     /translation="MTQAVLLTGTIGVGKTTVAEAISESLHDAHQLHALIDLDWLAQL
                     YPAPDEADPYNTNLAADNLAAVWRNFHTAGARRAVMAATVESAEQLGEIRRALPDAEF
                     TVVLVTASSDSVHGRIATRDRGRLRNDFLACTNALATRIESAGLHEFAIENDGRLPDE
                     AANEILQKLGWLP"
     gene            complement(3156..3284)
                     /locus_tag="VNP73_01425"
     CDS             complement(3156..3284)
                     /locus_tag="VNP73_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64608.1"
                     /translation="MKIHSGSMEICPCDWQEERRLQEDRDDEYDGAPPKMRGKKRG"
     gene            complement(3281..>3899)
                     /locus_tag="VNP73_01430"
     CDS             complement(3281..>3899)
                     /locus_tag="VNP73_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HWL64609.1"
                     /translation="GLTYSEYARQGFFQLLAVAVITSGVIAAVVRLADLSDTSMRRVA
                     RWSLGLLCALTLIVLLSASVRLSLYEEAFGFTRLRLLVHATIAGLAIVFVMIFVAGAI
                     WNGRWLTRGVFLLTGVGLVAFNLINPDLFIAQRNLERARTTGVLDTVYLAGLSEDAVP
                     ALATMSDLYPACAVVEDDNAAVWSFNLSRWEAEKLLSNLAPSGCQ"
BASE COUNT          801 a         1243 c         1140 g          715 t
ORIGIN      
        1 aatcatcgaa cctgggtgga catagctggc tggttgttgg gcgctctttt cttgatcggc
       61 ctttggaggg gccgacgatg ggccttcacg ctgtggttcg tgttcacttc gctcgcgctc
      121 gtggtttccg cgatcgcgta cgtgttcatc cccttgccgg aagcggcacg cttcccgatc
      181 gcggagacgg tcctctctct cattcaactc gcgttactga tgcaccccgc caccaagcgc
      241 ttcgccggca ccgagagatc ccccctgcct caggccgatg cacccactcc agcgcaatcg
      301 gattactaat ctcatccttg actggcggaa cacttgtctg cccggggagg ggcgcgacgg
      361 atgaaccatg accatcgact ctctgtccgc tcaactccag gctcatatcg agcgccacgt
      421 cggctgacta gccctcgtcg gagtggaaca ctcccgcaga tcgcccacca gacctctcgc
      481 ctcgagggaa ctccacgttt acagcaatcg agttgaccgc ctgaacctct gcaacggtct
      541 tgtaagcgag gctgtggaaa tacgtccccg cgtccaacga agtgagctcg acgacgacct
      601 cgcgattcgg ccctgtgggt agcccgaagg cagtcgaacc cgcacccgag ccgtcacacg
      661 aagtgaagaa gaacccgacc gagagatgag ctcggaccgg gtcgctccgc ttctcctgaa
      721 gacaggtctc gatctctccg taaagtcgtc cctccgcctc gacctcgacc gccaagatcg
      781 cccagcccat gcccccaagg gtttgcttcg cctcagccga gtagacgttg tgctcgggca
      841 acggatcgaa cgtggtcttc ggcgctgcta catccacgtc ttgcgtggcc gacaggtctt
      901 gcgtcgtctc gccactcaag ccggagaatg tcaggtcgac atcggttggg tacccgtccg
      961 ttatcagacg cacgaagtaa tctcccgacg gcagcgtgac attccgaggg aagccatggt
     1021 tagacggatc ccacacctgg gtgtagttca gcctgcgctc tttgcacggt ggtttcgcgc
     1081 ataacgcgac actcaacgca atgatctcga ctccgttctt cgggacgtcc tgcttcaaca
     1141 tcagaccggc tacctgtccg gtcgagcgga tgcgagccag tttgtttccg aagggatccg
     1201 tggagacctt cacgctctct gtgaggtgca gaaccgcctc gccggagcgc gtactcgaga
     1261 aatgattcgt acctgcgaac gcggctatcg gtggagccga tgcattcgac gttgggagga
     1321 cgaccacaag cagaacaagg agctggccaa cgacaacgaa ggatctcttc acacatagaa
     1381 cttcggtgtc cttgcgacat ccccttcgca ggattccctg tgactacggg ggagtcgtgt
     1441 gcaaagctct ttcttgtgag ggcgatgaga acgatcggtg ccgggctact ttcactcgct
     1501 cttctacttg ctatgcaacc ctccacagtc gctcaggctc ctcaggcgtg tacttggtcc
     1561 atcggcatca tgggcgctct cagcggcgac tacaaacgga tcggcgaacc catggcgaat
     1621 gggatccggc tcgcagccga tatcgccaac cgggaaggcg gcctgccgtg cacactgacg
     1681 acacgagctg aagacagtca gggggatccg aatcagtccc cacgcaaggc gcgcgcccta
     1741 accgaggatc agcaagtggt cgcctgcatc tgcggcttct tttccggtga gacgttggcg
     1801 agcggaaacg tcttcgaacg cggcggcctc atcatggttt ccacgggcac caaccgcacg
     1861 atcaagaaac aagggttcga tacctggttc cgtgcgatcg ccgccgaccc acgacaggct
     1921 cgagcaactg tcgcctacat caaggaaaac ctcagggccc gcaaagtagt caccgtccac
     1981 gacggccagg actactcgga gggactggcc caggatgttg gtgatgcact tgggtctcgg
     2041 cttcgcgcta ggtaccggat caatcccgag gagagcgatt acagcgcggt cgtatccaag
     2101 ataaagcgcg tcgaccccga cgtcgtgtac tacggcggct acaccccgca ggccggcgcc
     2161 cttctcaggc agctccacga ggctcgggtc ggagcaagat tcgtggtacc cgatggttcg
     2221 ctcgatgacg agttcatggg ccgggccctc ggccgcagaa atgtcgacat ggaagccagc
     2281 tgcccctgct cggacgcgca gaagatcgaa cgagctcaag agttcgctcg ggagtacgaa
     2341 gacgcctacg gcgaagaacc cggtatctac tcggccgata ccttcgacgt tgccaatatc
     2401 gcgatcgacg cgttgggcga gttgaccggc ctcgaaacga ccgagcaggt acgcgcccac
     2461 gtcgttgaat acttcgacaa cgtcgaacac gccgcgggca cggtgaagtc atatgactgg
     2521 tcggatcgag gcgagctcaa ggcgggcgcg gaccacgtat tcgtctggaa gtgggacaac
     2581 ggcacacgga aattcgagat gctagggcgg gtctcggaat tgacgtccta gaaccctatg
     2641 acccaagccg ttctgctcac cggcacgatc ggcgttggaa agaccaccgt ggccgaggcg
     2701 atctctgaat cgcttcacga cgcgcaccaa ctccatgcac tgatcgatct ggattggctg
     2761 gctcagcttt acccggcccc cgacgaagcc gatccctaca acacgaatct cgcggccgac
     2821 aacctcgcgg ccgtgtggcg caacttccac acggccggcg cacgccgcgc ggtgatggcc
     2881 gcgaccgtcg agtccgccga acaactcggg gagatccgaa gagcgttgcc ggatgccgaa
     2941 ttcaccgtgg tcctcgtgac cgccagttcc gactcggtgc acggacggat cgccacacgg
     3001 gatcgcggca ggctgcgcaa cgacttcctg gcctgcacca acgcgctcgc aaccaggatc
     3061 gagagcgccg gactgcatga gttcgcgatc gagaatgacg gccggcttcc ggacgaggct
     3121 gcgaacgaga tcctgcagaa gctcggctgg ctgccctagc ctcgcttctt ccctcgcatc
     3181 ttcggcggcg cgccgtcgta ttcgtcgtca cgatcctcct gtagacgacg ttcctcttgc
     3241 cagtcgcaag gacagatctc catagacccg gagtggatct tcattggcaa ccggatggtg
     3301 cgagattgct cagcagcttc tcggcctccc atcgggacag gttgaacgac cacaccgcag
     3361 cgttgtcgtc ctcgaccaca gcgcacgcgg gataaaggtc gctcatagtg gccagcgccg
     3421 ggactgcgtc ctcactcaag cctgctaggt aaacggtgtc cagcactccc gtggttcgag
     3481 cacgctcgag gttccgttga gcgatgaaca ggtccgggtt gatcagattg aaagcaacga
     3541 gccccactcc ggtaaggagg aacacaccgc gcgtcaacca tcggccattc cagatcgcgc
     3601 cggccacgaa gatcatgacg aaaacgattg caagccccgc gatcgttgca tgcacaagca
     3661 gcctcaagcg cgtgaacccg aacgcctcct cgtagagcga tagtcggaca ctcgcggaca
     3721 gtaggacgat caaggtcaag gcgcacagga ggcccagaga ccatctggcc actcggcgca
     3781 tgctcgtgtc ggaaagatcg gccagtctca ccacggcggc gatcacaccc gacgtgataa
     3841 cagcgacggc gagaagctgg aagaatccct gccgtgcgta ctcggagtag gtgaggcct
//
LOCUS       DATMED010000057         3959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_424212, whole genome shotgun sequence.
ACCESSION   DATMED010000057 DATMED010000000
VERSION     DATMED010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424212"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VNP73_01435"
     CDS             <1..651
                     /locus_tag="VNP73_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HWL64610.1"
                     /translation="DKDFATCLYRTQQMRAGASLTAITDDIRRSLGVGSVIIPMSDEP
                     VPTKIVTEDGRTLEFQDYFVKERHAPEVREVRFSGLADAKPAPGVLDAIRRADRVIIC
                     PSNPVVSIGPILALPEVRETLCDHACVIAVTPIINGAPLKGPADKLIPTLGAEVSASG
                     VAGLYRDFCDVFVVDERDAEEATLLGSEGTRSVTLDTVMSDRDASARLAAELLAID"
     gene            644..1333
                     /gene="cofE"
                     /locus_tag="VNP73_01440"
     CDS             644..1333
                     /gene="cofE"
                     /locus_tag="VNP73_01440"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWL64611.1"
                     /translation="MTDIQILGITGIPEVTAGDDVGLLLVDAFGVDSFRADDVVVVTQ
                     KVVSKAEGRLVHDEDKDAAIAGESVRILRRTVGGMTISETKHGFVCANAGVDSSNVQE
                     GHIALLPVDPDASARRIRSRIQHLTGVDVGVVISDTFGRAWRLGQTDVAIGVAGIDPF
                     LDYRGSTDAQGRTLNATRIAIADELAGAAELAMGKVENVCAAVIRGAAIKRGRGRATE
                     LVRPPGEDFFR"
     gene            complement(1345..1932)
                     /locus_tag="VNP73_01445"
     CDS             complement(1345..1932)
                     /locus_tag="VNP73_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWL64612.1"
                     /translation="MEFQEVVRRRRMVRNYTDEPVARATIERIVGHARRAPSAGFSQG
                     MYFVVVTRSDTRRSIAALAGEDEYAAKGFDRWISTAPVHVAVCVSEADYHARYSEPDK
                     LADGNELEWPIPYWWVDAGCSLMLLLLSAVAEGLAAGFVGTHRVGGLNELLGIPEAKI
                     PIGVVTIGYPAADRRSSSLGRGRKPAEEVVRWEKW"
     gene            1961..2626
                     /locus_tag="VNP73_01450"
     CDS             1961..2626
                     /locus_tag="VNP73_01450"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HWL64613.1"
                     /translation="MSKKLEQKQARRLEEESRKAARRKDAVRRNLVTIIVAVVVAAAV
                     VIAIASEKQTKEKLVGVSVQEAGCDPVEEFEGREGQHIKEGEPHDPYNSNPPTTGPHY
                     ESPADTGFYSSEIEAERVVHNLEHGQIVIYYHPDLSAEDQDLIEEIVDDDRIATVATP
                     YPSVDDKYAFEMTAWLAGEGEDDPGTGVVQGCAEVSQAAINEFRKEYQGKSPEPLTPR
                     FNG"
     gene            complement(2633..3604)
                     /locus_tag="VNP73_01455"
     CDS             complement(2633..3604)
                     /locus_tag="VNP73_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007468379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWL64614.1"
                     /translation="MRVMVTGAAGFIGSHLVDRLLGEGEEVLGVDDLSSGSLGNLGDA
                     RSSRLGKFTFQRVDITSTALADLIKRTKPELICHLAAQVDVRKSVKDPIHDAMVNLIG
                     TLNLLQGATEAGTRKVIFTSSGGCIYGEPDESRLPVTEDQVFSPEAMPESPYGVSKKV
                     AYDYLRYFKAVQGLDYTALALSNVYGPRQEPAAEVGLEGQVVAIFCRKMLSGRPCTIY
                     GDGSQTRDFLYVDDVVSGFLAAREKGSGELINLGSGTELSVDDLYSKLATLTGTRFDP
                     IYAAARPGELQRIVVGADKAGEVLGWSPQVTLDDGLKQTVAWFKATS"
     gene            complement(3615..>3959)
                     /locus_tag="VNP73_01460"
     CDS             complement(3615..>3959)
                     /locus_tag="VNP73_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWL64615.1"
                     /translation="YWLDLGTPEKYLQANMDALAGRYVTTSVSLTAEGSSVIGEGARI
                     DDAAKVSSSCIGPDSVVEEGASVAGSVLLPDVKVDRGATVRDSVLGQGVIVAPGADVT
                     GRTIGDNEKVEE"
BASE COUNT          766 a         1293 c         1210 g          690 t
ORIGIN      
        1 gataaggact tcgcaacctg cctctacaga acacaacaga tgcgcgccgg cgcgtctctt
       61 accgcgatca cggatgacat cagacggtcg ttgggcgtcg gatctgtgat catcccgatg
      121 tcggacgaac cggtgccgac caagatcgtg accgaagacg gacggacgct cgaattccag
      181 gattacttcg tcaaagaacg tcacgcaccc gaggtcaggg aggtgcgctt ctcaggattg
      241 gcggacgcga agccggctcc cggagtattg gacgcgatca gacgagccga tcgagtgatc
      301 atctgcccga gcaacccggt ggtctcgatc ggtccgatcc ttgcattgcc cgaggtgaga
      361 gagacgttgt gcgaccacgc gtgtgtcata gcggtcacgc ccatcatcaa cggcgcccca
      421 ctgaaggggc cggccgacaa gctgatcccg accctggggg ccgaggtaag cgcctccggg
      481 gtcgccggtc tctaccgcga cttctgtgac gtgttcgtcg tggacgaacg agatgccgag
      541 gaggcaacgc tcctcggatc ggaggggacg aggtcggtaa cactcgatac cgtcatgtcc
      601 gaccgcgacg cctccgcccg actcgcagcg gaactgttgg ccattgactg acatccagat
      661 cctagggata accgggatcc ccgaggtgac cgcgggtgac gacgtgggcc ttctgctggt
      721 cgacgcgttc ggcgtggatt cgttccgggc ggacgacgtg gtggtggtca cccagaaggt
      781 cgtgtcgaaa gccgagggca gattggtaca tgacgaggac aaggacgcgg ctatcgccgg
      841 cgaatccgtt cgcatcctga gaaggaccgt cggggggatg accatctccg agacgaagca
      901 cggtttcgtg tgcgcgaacg ccggcgtgga ctcgtcgaac gtccaggaag gccatatcgc
      961 gctgctgccg gtcgaccccg atgcgtccgc ccgccgcatc cgctcgcgca tacagcacct
     1021 gaccggcgtc gatgttggcg tcgtgatcag cgatacgttc ggtcgagcct ggcggctggg
     1081 ccagacggat gttgccatcg gagttgctgg gatcgacccg ttcctcgact accgcggcag
     1141 caccgacgcc cagggtcgga ccctgaacgc aactcgcatc gcgatcgccg acgaactcgc
     1201 aggagcggcc gagctcgcga tgggcaaggt agagaacgtc tgcgccgcgg tgatccgtgg
     1261 ggcggcgatc aagcggggac ggggccgtgc gaccgaactc gtgcgaccac ccggcgaaga
     1321 cttcttccgt taaccggctg cccttcacca cttttcccag cgaacgactt cctcggccgg
     1381 tttacgccct cggcccaggg acgacgagcg ccggtcggcg gccgggtatc cgatcgtcac
     1441 cacgcctatc gggatcttcg cctcggggat gccgagaagc tcattgagcc cgcccacgcg
     1501 atgggtaccg acgaagccgg ccgccaggcc ttccgcaacg gcgctcagca gcaacagcat
     1561 cagcgagcaa cccgcgtcca cccaccaata agggataggc cactcgagct cattcccgtc
     1621 ggccagcttg tcgggttcgc tgtagcgcgc gtggtaatcc gcttcgctca cgcacacggc
     1681 tacgtgaacg ggggcggtcg agatccaccg gtcaaacccc ttagcggcgt actcgtcctc
     1741 gcctgccagc gccgcgatcg agcgcctcgt atcggatcgg gtgacgacca cgaagtacat
     1801 cccctgggag aacccggcgc tcggcgcccg ccgggcatgg cccactatcc gctcgatcgt
     1861 ggctcttgcg acgggctcgt cggtgtagtt gcggaccatc cgtcgccgcc tgaccacctc
     1921 ttggaattcc aagcttcctc ccctgttagc ctctccggcc gtgagcaaga agctagaaca
     1981 aaagcaagca cgacggctcg aggaggagtc tcgcaaggcc gcgcgtcgta aggacgccgt
     2041 gcgccgcaac ctggtcacga tcatcgttgc agttgtcgta gccgctgcgg tcgtgatcgc
     2101 catcgcttcc gagaagcaga ccaaagaaaa gctcgtcggc gtgtccgtcc aggaagccgg
     2161 ctgcgatccg gtcgaggagt tcgaaggccg ggaaggccag cacatcaagg aaggcgaacc
     2221 tcacgatccc tataactcga acccaccaac aaccggcccc cactacgaga gcccggccga
     2281 taccggtttc tactcgtcgg agatcgaggc cgagcgcgtg gtgcacaacc tcgagcatgg
     2341 ccagatcgtc atctactacc accccgatct gagcgccgag gaccaggacc tgatcgagga
     2401 gatcgtcgac gacgaccgca tcgcgacggt tgccacgccg tacccatccg tcgacgacaa
     2461 gtacgcgttc gagatgaccg cgtggctcgc aggagagggc gaagacgacc cggggaccgg
     2521 tgtcgtgcag ggatgtgccg aggtttcgca agccgcgatc aacgagttcc gtaaggagta
     2581 tcagggcaag agccccgaac ccctcacccc tcggttcaac gggtaggtcg gatcagctcg
     2641 tagctttgaa ccatgcaacg gtttgtttca aaccgtcgtc cagggtcacc tggggagacc
     2701 atcccagcac ctctcccgct ttgtccgcgc ccacgacgat ccgttgcagc tcacccggcc
     2761 gcgcggccgc atagatcgga tcgaaccgcg tacctgtaag tgtggccaac ttggaataga
     2821 ggtcatcgac gcttagctcg gtcccgcttc cgaggttgat gagctcgccg ctccccttct
     2881 cccgggccgc aaggaatccg gacacgacgt cgtcgacgta aaggaagtcg cgcgtctggc
     2941 tgccgtcccc gtagatcgtg cagggcctcc ccgacaacat cttgcggcag aagatggcca
     3001 cgacctgccc ttcgagtccg acctctgccg ccggttcctg acgtggaccg tagacatttg
     3061 atagcgccaa tgcggtgtag tcgagaccct gtaccgcctt gaaataacgc agatagtcgt
     3121 acgccacttt ttttgaaacg ccgtaaggag actcgggcat ggcttccgga ctgaagacct
     3181 gatcctcggt aaccggcagg cgcgactcgt cgggttcacc gtagatgcag cctccggagg
     3241 acgtgaagat gaccttccgc gtgccggcct ccgttgcccc ctgcaagaga ttgagcgtgc
     3301 cgatcagatt caccatcgcg tcgtggatcg gatccttcac cgatttgcgc acgtcgacct
     3361 gcgcggcgag gtggcagatc aattcgggtt tggtccgttt gatgagatcg gcaagcgccg
     3421 tcgaagtgat gtccacgcgc tggaaagtga acttgcccaa ccggctcgac cgagcgtccc
     3481 ccaggtttcc aagtgagccc gacgacaggt cgtcgacgcc gagtacctct tccccttcgc
     3541 ccagcagtcg atcgacaaga tgagagccga tgaaacctgc cgcacctgtg accatcactc
     3601 gcatgactta gcgatcactc ctcgactttc tcgttgtctc cgatcgttcg accggtgacg
     3661 tcggcacccg gggcaacgat cacgccctgc ccgagcaccg agtccctgac cgtcgctccc
     3721 cggtccacct tgacgtccgg gagcagaacg gatccggcga cggaagcccc ctcctcgacg
     3781 accgaatccg ggccgatgca ggaggaggat accttcgccg cgtcgtcgat ccttgcccct
     3841 tcaccgatga ccgacgaacc ctcagcggta agcgacactg aagtcgtgac ataacgtccc
     3901 gcaagcgcgt ccatgtttgc ctgtaggtac ttctccggag tcccaaggtc gagccagta
//
LOCUS       DATMED010000058         7734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4651213, whole genome shotgun sequence.
ACCESSION   DATMED010000058 DATMED010000000
VERSION     DATMED010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4651213"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VNP73_01465"
     CDS             <1..771
                     /locus_tag="VNP73_01465"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWL64616.1"
                     /translation="EPLGDGRYRAELDDAWWVVRGPHGGYLAATILRALTRSLGDSDR
                     PIRSFTTHFMAAPNSGSLEIETRLERAGRSMTFVTARAEQEGRPIAMSLAAFSTDWKG
                     IEFDNAPMPEAASIDDAFPNPIEGEGIPAFLGNFDMRWALGERPFTGAPRAEVGGWMK
                     MREPVVADEHVIACLLDAWAPAVFPVATTPLVAPTIDLTMHFRTALPLTGATPDDFYL
                     GRFSSSLGAEGFFEEDGAIWSSDGNLIAQSRQLALGLI"
     gene            complement(800..1906)
                     /locus_tag="VNP73_01470"
     CDS             complement(800..1906)
                     /locus_tag="VNP73_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64617.1"
                     /translation="MVWLLTAAVLSTSLIAVPARGTGLTSDNVTYLKTVPLEAGVATG
                     ARIVGKHLYVAGAKSFSIYDISDPENPALLSYTPIGAAFAVEDVDTNGKILLIHDEQG
                     ASRVEGGKLSIWDVENKSAPAKIAEVTGVNSHTFSCVLKCSYAYGSRGHIIDLRKPEE
                     PKLVGSWGGLQPNDGFDVTEVAPGLILTASRVMYFLDARKDPANPTILAKGSTHDNRL
                     IHSNRWPNAGRDRFYLVQGETPLSQTCDEASGAFMTWDASKWRRTHSFSMIDEFRVGN
                     GTYTDGNPPAGAWGCTTMWFDDHPNFNNGGLVVSGFFEHGARFLKVDTRGKISEIGYF
                     TPAAGETIATYWVTEDIVYAIDLHRGIDILRFAS"
     gene            2048..3190
                     /locus_tag="VNP73_01475"
     CDS             2048..3190
                     /locus_tag="VNP73_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64618.1"
                     /translation="MRKLMLVLSLVLAVGAAVPAAEAGPTQGGYTSDNVEYLSHVSFE
                     AGTATGMKVVGDYLYITSWKTFSIYDVSDPLNPEQMSMTPFGFKFENEDVATNGKIML
                     FSEQLPQNILHIWDVEDPESPQEIAQLEGAGGHTSDCILDCKYSYASGGVITDLRNPA
                     KPKMVGDWSIDPKTKKDRPAASAHDVNEVAPGMVLTSSRPIMYLDGRKDPAKPSLIAL
                     GDDDKITGGIHSNQWPNNAKDDFVMFSSESNGTGRCTGSNGAFMTWDARHYKKTNSFT
                     LLDIYQLSNGTYQDGSPPVNGLGCSAHWFEQHPTFKNGGIVALGSYEHGTRFVDIAPG
                     TGKIKEIGYFVPWGGSTSAAYWLTDEIVYAVDYSRGIDILRFKAKA"
     gene            complement(3187..3885)
                     /locus_tag="VNP73_01480"
     CDS             complement(3187..3885)
                     /locus_tag="VNP73_01480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL64619.1"
                     /translation="MFAQLGGEVHDVQVKGPIEFESSLKDGTRLHFRRIAPADKGRLV
                     EGFERLSPQSRYMRFFRHIDHLSDAQLDYLTDVDFEDHFAVVATLPDEVGEPICGVGR
                     WVRTTDDPKVAEGAVVVADYLHNQGIGRTLLWLMAGSAIERGVTAFRAWTLGDNHPML
                     QVLHDMGARAGRWESGVLELLVPLPEDPDEMEKTPAPLVLKATAAGVVHGEADPARPA
                     ATRLVPPPTFVPKN"
     gene            3856..5202
                     /locus_tag="VNP73_01485"
     CDS             3856..5202
                     /locus_tag="VNP73_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWL64620.1"
                     /translation="MDLASELREHVGDKYVLGSPDEKAPYLTDRSGRLTGAAQVVVLP
                     GDTGEVAAAVACCERHGVSVVPQGGNTGLAGGGVPESGSVVISLERLRDVAPVDPYTS
                     TVIAGAGVTLAELDDHVGGAGLFFPIDHAARSAATVGGTIATNAGGALAWRFGMARSC
                     VVAVEVVLAGGRVLTGLDRPLKNNSGLDLTGLMCGSEGTLGVVTRARLRLVPASPRRV
                     TALLPLSDLSEIAQVFAGLRDRCPSLLAVDFMSAEALDLVRSFADLPAAPFDAPIYLC
                     VECGAVEDPTDQLGAALSDLGLMKNAVAQDGEGRRRLWAYRELLNDAVTASGGHKMDI
                     ALPLTGLGDFLTAVNDQVSEIAPSARTVWFGHAGDGNVHLNVLDGHEHRDAIEERVYK
                     KVAAAGGSISAEHGIGRLKTRWLDLARSQEEIDAMRSIKQALDPHGMMNPGVMFTS"
     gene            5199..5600
                     /locus_tag="VNP73_01490"
     CDS             5199..5600
                     /locus_tag="VNP73_01490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWL64621.1"
                     /translation="MNTELIEGLISGAPLPSLLGIRLDRCDKDVAVLRMPFKEQLVTI
                     ADVVHGGAISALIDTTATAAAWATDELPENMRGTTVALTVNFLTAARATDLTATGRVV
                     RRGRSLCFLEVTVTAADGNAVATGLITYKLG"
     gene            5672..7192
                     /locus_tag="VNP73_01495"
     CDS             5672..7192
                     /locus_tag="VNP73_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HWL64622.1"
                     /translation="MLRLRVPYVVLVCSLVALTPGWAHAAPCDEDDTHHGHGWHYGNE
                     QCDADDNEARARKDPGSRRAQQAPAQVVDGVVSTAVTPSTSLAQVGDTVTWTISITNE
                     TSADLADVVVVDAVPTEFAVLEAATSETTPATIERGFVTWTIPSLPVGAATVLTWDGR
                     VDSLGDLEALNSVTVSAPSLESTVTDASVFLGGSVDVLVRQSAQKPDWGTEQRTVKLN
                     FNRPPSSVAGGAADADAAPGLLPMTGAAVDGWIVLAGLLMLTGAVLLVRRRSMRTAAT
                     CLLIALVLAACTSDPEDTTQARPEASESPGDSEDRVLGKRIERNDAGEGDGRNELPET
                     ELPSDEVAEAPSEGTDTSDDPGPSADVVAQQPAPQPSTTVVEVPADPPAPVPLAATPG
                     DNTMTFEWDEPSRSVLSAASSRVLRAGAPTELLTALTDPQDHLGARVDLTNITDDRRL
                     HVAGHLVLEVAGAGGTTSFTSDAIDQVLDPGDSVAADFEFLLPSGSYSITARFVPN"
     gene            complement(7215..>7734)
                     /locus_tag="VNP73_01500"
     CDS             complement(7215..>7734)
                     /locus_tag="VNP73_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL64623.1"
                     /translation="GELAVDAVNSGTVDVVLMDVKLGEAMSGIEATRQIKAATPETKV
                     LVLSMFTDPATVAEAVKAGADGYLSKGTSRENVLRAIKDVVEGRSVLDPTVTEGVFGR
                     IGRPSSEALTDRELTVLQKLAHGQSTKQVADEIFVSEETVKSHLKQIFRKLGARDRTQ
                     AVAEALRRGLVH"
BASE COUNT         1367 a         2540 c         2452 g         1375 t
ORIGIN      
        1 gaaccgctgg gtgacgggcg ttaccgggcc gagctcgacg acgcctggtg ggtggttcga
       61 ggcccccacg gggggtatct cgcggccacg atcctgcgag cattgacccg atcgctcggc
      121 gactcggacc ggccgatccg gtcgttcacg acccacttca tggcggctcc gaacagcggc
      181 tcgctggaga tcgaaacacg cctcgagagg gccggccgat cgatgacgtt cgtgaccgcg
      241 cgcgcagaac aagagggccg gccgatcgcg atgtcgctgg ccgcgttctc gaccgactgg
      301 aagggcatcg agttcgacaa cgcgccgatg ccggaggcgg cttcgatcga tgatgcgttc
      361 ccgaacccca tcgagggcga gggcatcccg gcattcctcg gcaatttcga tatgagatgg
      421 gcgcttggcg agcgaccttt caccggcgcc cccagggctg aggtcggcgg atggatgaag
      481 atgcgcgagc ccgtcgtcgc cgacgagcac gtcatcgcat gtctgctcga tgcatgggcg
      541 ccggccgtgt tccccgtcgc gaccacgcct ctcgtggctc cgaccatcga cctcacgatg
      601 cacttccgca ccgctctccc gctgaccggc gcgacccccg acgacttcta tctgggcagg
      661 ttcagttcgt cactgggtgc agaggggttc ttcgaggaag acggcgctat ctggagttca
      721 gacggcaacc tgatcgcgca atcccgccaa ctcgcgctgg gactgatcta gcccttctta
      781 gcgatcgccg agccgatcgc tagctagcga agcgcaagat gtcgatgccg cggtgcaggt
      841 cgatcgcgta gacgatgtcc tcggtgaccc aataggtagc gatggtctct cccgcggccg
      901 gagtgaagta gccgatctcc gagatcttcc ctctcgtgtc caccttcagg aagcgagcgc
      961 cgtgctcgaa gaagccggac acgacgagac ctccgttgtt gaagttcggg tggtcgtcga
     1021 accacatggt cgtgcagccc cacgcgcccg ccggcgggtt gccgtccgtg taggtgccgt
     1081 tgccgacgcg aaactcgtcg atcatcgaga acgaatgcgt gcggcgccac ttggatgcat
     1141 cccacgtcat gaatgcaccc gatgcctcgt cgcacgtctg agacaacggc gtctcgccct
     1201 gaacgagata gaagcggtca cgtcccgcat tgggccatct gttcgagtgg atcaatcggt
     1261 tgtcgtgggt cgagcccttg gccaggatcg tcgggttcgc cggatccttc ctggcgtcga
     1321 ggaagtacat gacccgcgac gcggtgagta tcaacccggg agctacctcg gtcacgtcga
     1381 agccgtcgtt gggctggagc ccgccccagc tcccgacgag cttcggctcc tcgggtttcc
     1441 tcaggtcgat gatgtggccg cgtgacccgt acgcgtagct gcacttgaga acgcacgaga
     1501 aggtatggct gttaacgccc gtgacctctg cgatcttcgc cggcgcgctc ttgttctcga
     1561 catcccagat ggacagcttg ccgccttcca cccgggacgc cccctgctcg tcgtggatca
     1621 ggaggatctt cccgttcgtg tcgacgtctt cgacggcaaa cgcggccccg atcggggtgt
     1681 agctgagcag cgcagggttc tcgggatcgg agatgtcgta gatcgagaag ctcttcgctc
     1741 ccgcgacgta aaggtgctta ccgacgatcc gggccccggt cgcgaccccc gcttccagcg
     1801 gcaccgtctt cagataggtg acgttgtcac tcgtcagtcc cgttccgcgt gccggaacgg
     1861 cgatcagact tgtcgacagg acggccgccg tcagcagcca gaccatacgc cttagcagcc
     1921 agaccatacg cctttgcata tccatctcat tcgacgtcgc tgcgcctgct cctaccccgt
     1981 gtggcaggga ttcgggcatc cggcccgaat cctcacaaac aggacaacga tctcccggga
     2041 ggccgcgatg aggaagctga tgttggttct gagcctggta ctggccgtgg gggccgccgt
     2101 gccggcagcc gaggccgggc cgacccaggg tggctacacc tccgacaacg tggagtacct
     2161 cagccacgtc agcttcgagg ccggcaccgc caccggtatg aaggtcgtcg gcgactacct
     2221 ctacatcacg tcgtggaaga cgttctcgat ctacgacgtc tccgatccgc tcaaccccga
     2281 gcagatgtcg atgacgccgt tcggtttcaa gttcgagaac gaagacgtgg ccacgaacgg
     2341 caagatcatg ctgttctcgg agcaactccc ccagaacatc cttcacatct gggatgtcga
     2401 ggatcccgag agcccacagg agatcgcgca gctcgagggg gccggtgggc acacctcgga
     2461 ttgcatcctc gactgcaagt actcctacgc atccggtggc gtgatcaccg acctgcgtaa
     2521 cccggcgaag ccaaagatgg tcggtgactg gtcgatcgat ccgaagacga agaaggaccg
     2581 tcccgccgcc agcgcgcacg acgtcaacga ggtcgctccc ggaatggttc tgacatcctc
     2641 ccgtccgatc atgtatctgg acggcaggaa ggatccggcc aagccgtccc tcatcgcact
     2701 cggtgacgac gacaagatca cgggtgggat ccactccaat cagtggccca acaacgcgaa
     2761 agacgacttc gtgatgttct cttccgagtc gaacgggacc ggacgctgca ccggttcgaa
     2821 tggtgccttc atgacgtggg acgcccgcca ttacaagaag acgaattcgt tcacccttct
     2881 cgacatctac cagttgagca acggcacgta ccaagacggt tcgcctccgg tgaacggtct
     2941 cggttgctct gcccactggt tcgaacagca cccgacgttc aagaacggcg ggatcgttgc
     3001 gctgggttct tacgagcacg gaacccgttt cgtcgacatc gcaccgggca ccgggaagat
     3061 caaggagatc gggtacttcg ttccttgggg tgggtccacc tcggcggcct actggctgac
     3121 ggacgagatc gtctacgcgg tcgactactc gcgcggcatc gacatcctgc ggttcaaagc
     3181 caaggcctag ttcttcggaa cgaaggtcgg tggtggtact aggcgcgtgg cggccgggcg
     3241 agcgggatcg gcctcgccgt ggaccacccc tgccgccgtc gccttcaaaa ccaacggcgc
     3301 cggggtcttt tccatttcat cgggatcctc gggcagtggc accagcaact ccagcacgcc
     3361 ggattcccag cgccccgctc gagcgcccat gtcgtgcaac acctggagca tcggatggtt
     3421 gtcaccgagt gtccacgcgc ggaaagccgt gacccctcgc tcgatcgcgc tgccggccat
     3481 cagccacagg agcgtccggc ctatcccctg gttgtgcagg taatcggcca cgaccacagc
     3541 tccttcggcc accttcgggt cgtcggtggt gcgcacccag cgccccacac cacagatcgg
     3601 ctctcctact tcgtcgggaa gcgttgcgac gacggcgaag tgatcctcga aatccacatc
     3661 cgtcaggtag tcgagctgcg cgtcggacag atgatcgatg tgcctgaaga agcgcatgta
     3721 gcgcgattgg ggtgacagcc gctcgaatcc ctcgacgagc ctccccttgt ctgcgggagc
     3781 gatccgcctg aagtggagtc tcgtgccgtc cttcaaggag gactcgaact cgatcggacc
     3841 cttaacctgc acgtcgtgga cctcgcctcc gagctgcgcg aacacgtcgg cgacaagtat
     3901 gtcctgggat ctccggacga gaaggccccc tacctgaccg accgcagcgg gcgcctaacc
     3961 ggagccgccc aagtcgtcgt gctgcccggc gacaccggcg aggtcgcagc ggcggtggcg
     4021 tgctgcgagc ggcacggcgt ctcggtcgtt ccgcagggag ggaacaccgg gttggccgga
     4081 ggaggggtac cggagtcggg ctcggtagtg atctcactcg aacgtttgcg ggatgtagcc
     4141 ccggtcgacc cgtacacgag cactgtgatc gcgggggccg gagtgactct cgccgagctc
     4201 gacgaccacg tcgggggggc cgggctcttc ttcccgatcg accacgccgc ccgctctgcc
     4261 gccacggtcg gcggaaccat cgcgacgaac gccgggggtg cgcttgcgtg gcgtttcggg
     4321 atggcgcgtt cctgcgtcgt cgccgtcgag gtcgtgcttg ccggcggtcg agtgctaact
     4381 ggactcgacc ggccgctcaa gaacaactcc gggctcgatc taaccggctt gatgtgtggg
     4441 agcgagggca ccttgggcgt cgttacgcgt gctcgtcttc gactggttcc tgcgtcacca
     4501 cggcgcgtga ccgctcttct tccgctgtcg gacctctccg agatcgcgca agtgttcgcc
     4561 ggactgagag atcgatgccc gtcgttgctt gcggtcgact tcatgtccgc ggaggcgctc
     4621 gacctcgtcc gttctttcgc cgacctgccc gcggccccct tcgacgctcc catctacctc
     4681 tgtgttgaat gcggagccgt cgaggatccg accgatcagt tgggggccgc tctcagcgat
     4741 ctcggtctca tgaagaacgc tgtggcgcaa gacggcgagg gccggcggcg gctgtgggcc
     4801 taccgggaat tactgaacga tgccgtcact gcatccgggg gccacaagat ggacatcgca
     4861 ttgccgctca ccgggttggg cgacttcttg accgctgtga acgatcaggt ctcggagata
     4921 gcgccctcgg cgcgcaccgt gtggttcggt cacgcaggcg acgggaacgt ccatctcaac
     4981 gtgttggacg gacacgagca ccgggatgcg atagaagagc gcgtgtacaa gaaggtggct
     5041 gcagccggtg ggtctatcag cgccgagcac gggatcggcc ggctcaagac gaggtggctc
     5101 gacctcgccc gttcgcagga agagatcgac gccatgaggt ccatcaagca agcgctggat
     5161 cctcacggca tgatgaaccc gggggtgatg ttcacttcat gaataccgag ctgatcgaag
     5221 gattgatctc gggagccccg ctcccctctc tcctcggcat acgactcgac cggtgcgaca
     5281 aagatgtcgc cgtcctgagg atgccgttca aggagcaact cgtgacgatc gccgacgtcg
     5341 tgcacggtgg agcgatctcg gccctcatcg atacgacggc aaccgcggcc gcgtgggcga
     5401 ccgacgagct gccggagaac atgaggggaa cgaccgtggc tctcaccgtc aatttcctga
     5461 cggccgcacg agcgaccgat ctcacggcca cgggtcgagt cgtgcgcagg ggccgttccc
     5521 tgtgcttcct ggaagtgacg gtgaccgcgg ccgatggaaa cgccgtggcc acgggcttga
     5581 tcacatacaa gttgggctag atgggttgaa acagaccaag cggccagtaa ttaacaggaa
     5641 aacggcgctc gcccccatta gattgagagc gatgcttcgt ctcagggtcc cttacgtcgt
     5701 tctggtctgc tcgctcgttg ccctgactcc gggctgggcg cacgcggccc cctgcgacga
     5761 ggacgacacg caccacggtc acggttggca ctacgggaac gaacagtgcg acgcggacga
     5821 caacgaagcc cgcgctcgca aagacccagg ctcacgtcga gcccaacagg cgcctgccca
     5881 agtcgtcgac ggggtcgtct ctactgcggt gacacccagc acctccctcg cacaggtcgg
     5941 cgacacggtg acgtggacca tctcgatcac gaacgagacc tcggccgacc tcgccgacgt
     6001 cgtggtggtg gacgcggttc ccaccgagtt cgccgtcctg gaagcggcaa catcagaaac
     6061 aactcccgcg acgatcgaac ggggcttcgt cacatggacg atcccgagtc tcccggtagg
     6121 ggcggcgacg gtcctcactt gggacggacg ggtcgactcg ctgggtgatc tggaagcgct
     6181 caactccgtc acggtgagtg ctccttcgct cgaatccacg gtcaccgacg cgagcgtctt
     6241 cctcggcgga agtgtggatg tccttgtgcg gcagtcggcg cagaagccgg attggggcac
     6301 cgaacagcgg accgtgaaac tgaacttcaa tcgccctccg tcgagcgttg caggcggagc
     6361 cgccgacgca gacgcggccc ccggcctcct tcccatgacc ggagcggcag tcgacggttg
     6421 gatcgtcctc gcggggctcc tgatgctcac cggggcggta ctcctggtcc gcagacgatc
     6481 gatgcgcacg gcagccacgt gcttgttgat cgccctcgtc ctggcggcct gcacgtccga
     6541 tcccgaggac acaacgcagg ctcgcccgga ggcaagcgag tcgccgggtg actcggagga
     6601 ccgcgtgttg ggcaagagga tcgagcgcaa cgacgccggc gagggtgacg gccgcaacga
     6661 gcttcccgag accgaacttc cttcagacga agtggcagag gcccccagcg agggcacgga
     6721 cacgtccgac gacccgggcc cgtccgccga tgtcgtcgca caacagccgg ccccgcaacc
     6781 ctcgaccacg gtggtggagg taccggccga tcccccggcc ccggtccccc tggcggctac
     6841 tccgggcgac aacacgatga cattcgaatg ggacgagccg agccgctcgg tcctgagtgc
     6901 ggcctcgtcg cgcgtccttc gagccggggc gcccaccgag ctactgaccg ccctgaccga
     6961 tccgcaggat cacctcgggg cgcgcgtcga cctaacgaac atcaccgacg accgacgcct
     7021 ccacgtcgca ggtcacctcg tcctcgaggt cgcgggcgcg ggtggaacga catcgttcac
     7081 gtcggacgcg atcgatcagg tgttggatcc aggagattcg gtcgccgccg acttcgaatt
     7141 ccttctgccg agcggctctt acagcatcac ggcgcgcttc gtaccgaact agcggcggcg
     7201 ctcagaggaa gcgtctagtg aacgaggccc ctacggagtg cctcggcgac ggcctgtgtc
     7261 cggtcccgcg caccgagctt cctgaagatc tgcttcagat ggctcttcac ggtttcctcc
     7321 gacacgaaga tctcgtcggc cacctgcttg gtggattgcc cgtgcgcgag cttctgcagc
     7381 acggttaact ccctgtcggt aagtgcttcc gaactgggcc tgccgatccg accgaacaca
     7441 ccctccgtca cggttgggtc gaggaccgag cggccttcta cgacgtcttt gatggcgcgc
     7501 agaacgttct cgcgcgaggt ccccttcgat aggtaaccgt cggctcccgc cttgacggct
     7561 tcggcgaccg tggcgggatc ggtgaacatg ctgagcacga gaaccttggt ctcgggagtg
     7621 gcggccttga tctgccgcgt ggcctctatc ccactcatcg cctcgcccag cttcacgtcc
     7681 atgagaacga cgtcgacggt cccggagttg acggcatcaa ccgcgagctc cccc
//
LOCUS       DATMED010000059         3829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4651660, whole genome shotgun sequence.
ACCESSION   DATMED010000059 DATMED010000000
VERSION     DATMED010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4651660"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VNP73_01505"
     CDS             complement(<1..801)
                     /locus_tag="VNP73_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWL64624.1"
                     /translation="MLVRVIRICASLVLLCALFPLTASPVQADEPDYDSDRAIVGIKS
                     KDKVKAKDAVEELGGEVEEVSESGYFMVVETPEDSKNWAAKLKGDNRVKYVEPDYEVQ
                     ADALPTDPRYPELWAMPKIGMPAAWDLEDGSSSVIVGVNDSGIDYTHEDLASQMWIND
                     DPVNGVDDDGNGWVDDRHGIDCRNNDGNPIDDYDHGTHVAGTIGADANNGKGVAGVNW
                     NVSLMALKFLGSTGSGSTSDAAECLHYAIDNGAHVTNNSWGGGGFSQTM"
     gene            complement(921..2231)
                     /locus_tag="VNP73_01510"
     CDS             complement(921..2231)
                     /locus_tag="VNP73_01510"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWL64625.1"
                     /translation="METTTTLDAKAVGRRIRDLRRKRGISQEELGSPRYSHAYISHLE
                     RGTRRPSGEALGHIAARLDVSVEQLETGRDPHLDIQLQLDVDRAIAEIHSGHLDEAAA
                     KIEAVRKRARKEGFTEASAVAEEALALVAQRKGLWEEALAHVDAAEALLAGQPAERRT
                     SLVTTRARCLFASNDLNHAIHILERHLVELNQHGSGDPTALLQTYSALVGPYWEAGFK
                     DRAAAIAEEGFRLETRVQDPEHVACMHINRAQILLEQGHKGEAMRSLSRAEDLFKQIG
                     WRDSAVKAAVAQAVAALEHGDLEEAETRAHNALAEISASPSVLDQVRILNLLARIARV
                     REQPRLALKYLDEASELLGDSESLEQGWRFRETGLCYLDLEQPDPAVKALRAALQIYR
                     EASSPAQVATTAAYLGDALTRLGHHDEAGEVYRSGLAEIEDLTL"
     gene            2612..3088
                     /locus_tag="VNP73_01515"
     CDS             2612..3088
                     /locus_tag="VNP73_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64626.1"
                     /translation="MAAKKDPSPLVSIAASVPGEIVTGRIETAGDRYEVWIDDRKLGA
                     APDLKACLRTVGLFLRVALPPVEEGEWELDVSCPQSQRFGISLGNMGPMTWVGSKENT
                     CIENILNAMPLNTMSDAIEASMAAIATCCVAAGRLEGETEDLTDGGLTVGTKTRDP"
     gene            complement(3110..>3829)
                     /locus_tag="VNP73_01520"
     CDS             complement(3110..>3829)
                     /locus_tag="VNP73_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64627.1"
                     /translation="GKGADALFGGAGDDHLESGGGAEVYFGRDRADDLLDGGAGKDYF
                     DAHDVDKRGRATVQMVSLTRGIAVGEGRDQMRRVENVEVSGNGSVIRGDKRANLLQGW
                     GKISGGPGADALSHAKEVWGGRGKDLVFDVPIAHLGPGPDEFVGDLGGGNHEVYGGPG
                     DDDIRGGTGGEGADPSGAEADRFFGGPGDDELRGFVGADRLFGRGGDDFLNGGKGRDR
                     AKGGGGRDTCKAEDQRGCERD"
BASE COUNT          646 a         1231 c         1157 g          795 t
ORIGIN      
        1 catcgtttgc gagaaaccgc caccacccca agagttattg gtgacgtggg ctccgttgtc
       61 gatggcgtaa tggaggcatt cggccgcgtc actggtggat ccgctaccag tggagccgag
      121 gaacttgagt gccataagtg agacgttcca gttgaccccc gcgacgccct taccgttgtt
      181 ggcgtcggcc ccgatcgttc ctgcgacgtg agtcccatgg tcgtaatcgt cgatcgggtt
      241 cccgtcgttg tttcggcagt cgatcccgtg acggtcgtcg acccatccat tgccgtcgtc
      301 atcgacgccg ttgaccggat cgtcgttgat ccacatctgc gaggcgaggt cctcgtgggt
      361 gtagtcgatg cccgagtcgt taacgccgac gatcacactg ctagagccgt cctctaggtc
      421 ccatgccgcc ggcatgccga tcttgggcat ggcccacaac tcgggatagc gaggatcggt
      481 tggaagcgca tccgcttgca cctcgtagtc gggctcgacg tacttgaccc tgttgtcgcc
      541 cttcagcttg gcggcccagt tcttcgagtc ttctggggtt tcgacgacca tgaagtagcc
      601 gctctcggag acttcctcga cctcgcctcc gagttcttcg accgcgtctt tcgcctttac
      661 cttgtccttg ctcttgatac cgacgatcgc gcgatcggag tcgtaatcgg gttcgtcggc
      721 ttgtaccggg gatgccgtca gcgggaagag tgcgcacagc aacacgagtg atgcgcagat
      781 acggatgacg cgcacgagca ttgccgcccc ttcaaaggtt tctccgaccg tacgcagggg
      841 gccgcggcca atccatagcc caatcgggtc atatcgccac ggttgccgcg ccggatctcg
      901 gcaaagaacg gttgaggcgg ctagagggtt agatcctcga tctccgcgag gccggatcga
      961 tacacctctc cggcctcgtc gtggtgaccc agccgagtga gcgcatcccc caggtaggcc
     1021 gccgttgtcg ctacttgggc gggagagctg gcttcgcgat agatctgcag cgccgccctc
     1081 agggctttga cagcggggtc cggttgctcg aggtctaggt agcacaagcc ggtctcacgg
     1141 aaacgccagc cctgctctag cgattcggaa tctcccagca gctcggacgc ttcatcgagg
     1201 tacttcaacg cgagccgagg ttgctcccgc acccgcgcta tccgcgccag gagattgagg
     1261 atgcgcacct gatcgagaac gctcggcgat gcactgatct cagcgagagc gttatgtgcc
     1321 ctcgtctcag cttcttcgag atcaccgtgt tcgagggcag caacagcttg ggcaaccgcc
     1381 gctttgactg ccgaatcgcg ccatccgatc tgtttgaaca ggtcctctgc acgcgatagc
     1441 gagcgcatcg cctcgccttt atgaccctgc tcgagaagga tctgcgcccg gttgatgtgc
     1501 atgcacgcta cgtgctcggg gtcttgaacc cgagtttcga gcctgaagcc ttcttcggcg
     1561 atcgcggccg cacgatcctt gaatcctgcc tcccagtaag gccccacgag agcggaatag
     1621 gtctgcaaca acgcggtcgg atcgcccgat ccgtgctggt tgagctcgac caggtggcgt
     1681 tcgaggatgt ggatcgcgtg attcaggtcg ttcgacgcga agagacagcg cgctcgcgtc
     1741 gtaaccaatg atgtgcgtcg ctctgcaggt tggccggcga gtagtgcctc ggccgcgtca
     1801 acgtgtgcca gagcttcttc ccagagtccc ttgcgctgag cgaccaaggc gagggcttct
     1861 tcggctaccg cgcttgcttc ggtgaatcct tccttccgcg cccgcttgcg aacggcttct
     1921 atctttgcgg cggcttcgtc gaggtggccc gagtggatct ccgcgatcgc tctgtcgacg
     1981 tcgagttgca gctggatgtc gagatggggg tctctgccgg tctctagttg ttcgacgctg
     2041 acgtcaagac gggcagcgat atggcccaac gcttcgccgg agggccgccg cgttccgcgt
     2101 tcgagatgac ttatgtacgc atggctgtaa cgcggagaac cgagctcctc ctgagagata
     2161 cctcgtttgc ggcgtaggtc ccggatcctg cgacctacgg ccttcgcgtc cagcgttgtc
     2221 gtggtttcca tattcctcaa cataccgagc aggccacctt gtccttatct gtaactattt
     2281 atacagaaat cgtatagcta atggggaggt gacatgaaca agcgaatcgc gactgcgatc
     2341 gccgctctgg ccctcgtgct gacgggagca cagtctgctg cggccgaccc gctgaactgc
     2401 ctgctgaagc cctggtgcta attcgggctt aaacgcagga ttgcaccaaa aggaacagaa
     2461 attgttacat taggccctat ccggggcata tagaccgctg ggcgggttta cgccgtaagg
     2521 ggagtcgacg gccgcttggg ctcgggatgc ccgtggaacc gcatcccgga ccccggccgc
     2581 cggctagtgg ggagagagga gttgaccgac gatggcagct aagaaggatc cgtcccctct
     2641 tgtgtccatt gccgcgtcgg tgcccggcga aatcgtgacg ggcaggatcg aaaccgcggg
     2701 ggatagatac gaggtctgga tcgacgatcg caaactgggc gcggcgccgg atctcaaagc
     2761 gtgtctgcgc actgtcggcc tcttcttgcg cgtggccttg cctccagtcg aagagggtga
     2821 gtgggaacta gatgtgagct gcccacaatc ccagcgcttc ggcatctctc taggaaacat
     2881 ggggccgatg acgtgggtgg gctcgaaaga gaacacctgt attgagaaca ttctgaatgc
     2941 gatgccgctc aacacgatga gcgacgcgat cgaagcgtcg atggccgcga tcgcaacttg
     3001 ctgtgtagct gcaggccggt tagagggcga gactgaagat ctgacggatg gcggactcac
     3061 cgtcggtacc aagactcgtg atccgtagtt cagtcgctcc ttcctgccgt tagtcgcgct
     3121 cgcatcccct ctggtcctcg gccttgcagg tgtcccggcc cccgcccccc ttggcccggt
     3181 cacgcccttt gcccccgttc aggaaatcgt cgccgccccg cccgaagagg cggtcggctc
     3241 ccacgaagcc tctgagctcg tcgtcgccag gtcccccgaa gaagcggtcg gcctcggccc
     3301 cgctcgggtc cgcgccttcg ccgcccgtcc cgcctctgat gtcgtcgtcg cccgggcccc
     3361 cgtagacctc gtggttcccc ccacccaagt caccgacgaa ctcgtcgggg ccgggaccaa
     3421 ggtgcgcgat cgggacgtcg aatacgagat ctttgccccg tccaccccac acctctttgg
     3481 cgtgactgag cgcgtccgcg cccgggccgc cgctgatctt cccccaacct tgcaagaggt
     3541 tcgcccgttt gtctcctcgg atgaccgacc cgttgccgct tacttccacg ttctcgaccc
     3601 ggcgcatctg gtcgcgacct tccccaaccg cgatccctct ggtcaggctc accatctgaa
     3661 cggtcgcccg gccacgcttg tccacgtcgt gtgcatcgaa gtagtccttg ccagcaccgc
     3721 cgtcaaggag gtcgtcggcg cgatcgcgcc cgaagtaaac ctctgcaccg cctccgctct
     3781 cgaggtggtc atctcccgcg ccgccgaaga gcgcgtcggc ccccttccc
//
LOCUS       DATMED010000060         4600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5029644, whole genome shotgun sequence.
ACCESSION   DATMED010000060 DATMED010000000
VERSION     DATMED010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5029644"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VNP73_01525"
     CDS             <1..219
                     /locus_tag="VNP73_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64628.1"
                     /translation="SVEESPDVLFRKPGDHLDRRLLVLERLMVDVGRDSEDEATEDPV
                     GSHASYRSPEETELLGVRYPENDELEVL"
     gene            complement(525..598)
                     /locus_tag="VNP73_01530"
     tRNA            complement(525..598)
                     /locus_tag="VNP73_01530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Met,seq:cat)
     gene            complement(681..3524)
                     /locus_tag="VNP73_01535"
     CDS             complement(681..3524)
                     /locus_tag="VNP73_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWL64629.1"
                     /translation="MARAGGGRSSRRAIIVVTLLIVFIGSNFSAYFYTDLLWFKEVGF
                     TSVLWTSLGTQWGVGLATGVVVAALVWINLVVAARLAPAYRAGISIAGGSDPLQKYRE
                     AAAPYFRRLRFGAAAVVGFLIGAVASSNWETFLLYANRVEFGSVDPQFGRDISFYVFE
                     LPLYDLLVSEVWTTLFACLLITVGAHLLYGSIRAVRGLGGVAPGALAHVSVILGLLAL
                     TKSAQYWLGRFQLNFSSRGVVDGASYTDVHAQLPALNVLALISIVSAVLFLVNIRLRR
                     VSLPLAAVGIWILFSFVAGGIWPWSVQRFSVEPNELPRERPFLQRNIEATRGAFQLDD
                     VETRPFAASFDLTPEDLKQNQPLLRNVRVWDPGILQLAYIQLQAIRPYYEFPDVDIDR
                     YRVNGQLRQVLLSARELSIEHLEDRSKKWANEHLQYTHGYGLVASLANEATTAGQPDF
                     LVQGVPGTVEEGAESLAVEQARVYFGEAFEDREYSIVNTNQQEIDYPTDAGPERSRYE
                     GEGGVPVGTFGKKLAFAIREGDTNLILSDLITPESKIIFYRNVRDRVLRAAPFMQLDG
                     DPYVANVDGRLTWILDGYTSTPWYPYSERFDVGAILNSSFTGQPTGNVNYIRNSVKIT
                     VDAYDGTMKFYVIDDEDPLVAAWRNAFPELFTDEEPSMELREHFRYPEDLFKIQSEVY
                     TTYHIEDPNDYYSREDVWAIPFADQPVQETTALSSSVQSGLVPPTYLLIELPQEEGEE
                     FVLARPFTPRTKANMISFMVARADPDHYGELVSLQFPRQKLIPGPAQVHNLINQDVEF
                     SQTRTLLGREGSAVQFGSLITLPIEESILYVQPVFVISEGIGIPELKRVLLVFGEEVA
                     VGETFEEALADLFGDDVDVPEEPDLVEGDGDEEPEPDEGDEPRPGDSELQQILTQAAR
                     LYEQAQEALAAGDFEEYGRLIEQLGELLEQANP"
     gene            complement(3580..>4600)
                     /locus_tag="VNP73_01540"
     CDS             complement(3580..>4600)
                     /locus_tag="VNP73_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64630.1"
                     /translation="LEGDRILRMQSHSEMVISSTRSIRPTRGNILIDAGEPTRATFDG
                     VTASAREASFRVDRGFGTVRTGAYEGQVELASPGQSPLTVSRLFEATVAAGDLPGKSD
                     PYRVNEKDPWDLDRLQDVVELEEELGLLAKGFSRLVGNNKPNLAYFNALADGPGVSFM
                     RPYLKRRPVDLLIGFTIANNDRDHGLAGAFKDAFGFYDRGATWGVTAAIMEVSARPVV
                     AQLEDVILSTGVVAADGSGEEAVFSVASAEAADGGPAPSGPRGDAGQDPGGNDDRPAG
                     LGDDEGDDGNDGGDDDDGGEEECDPTDVGCQAGRVIPSPSPTDDPEEEEEEDEGLLTG
                     VVEDD"
BASE COUNT          895 a         1447 c         1406 g          852 t
ORIGIN      
        1 tccgtcgagg agtctcccga cgtcctcttc cgaaagcctg gcgatcacct cgaccgacgt
       61 ctcctcgtct tggagcggct gatggttgac gtggggaggg acagcgagga tgaagcgact
      121 gaggatcccg tcggatcgca cgcctcatat cggagtccgg aggagacaga gctcttgggt
      181 gttcgctacc cagaaaacga tgaactcgaa gtcctctgag tttgcgctgc gccaaccgga
      241 accaaactgg atctccctcg agtcccggcg gttctgatct ttgtagaaga cctcgagcgt
      301 ttccaccgag ccaaggctag agccctgacg agcctcgtgc tccaaaccgc gggtccggac
      361 ggtgtaggga aacagccacg tagaccagcg acagaccaac gaagctgtag tgagctatgt
      421 cagcgtccgc cattcgtaac cccccggcta cattccggcc gcaacatgca gaagccgcac
      481 gccatgccaa cggttgttcc tatcggtgac ctgcggcttc ctagttgcag gggcgggatt
      541 tgaacccgcg acctccgggt tatgagcccg gcgagctacc agactgctcc accctgcccc
      601 cgcatattaa cggagacggg tcctcgttct tttcgaagcc gcgccgcctg agatgggggc
      661 cggatgtgca gaaagaaggg ttaggggttg gcttgctcta ggagctcgcc tagttgttcg
      721 atcaggcgtc cgtactcctc gaagtcgccg gccgccagcg cctcttgtgc ttgctcgtag
      781 agccgggccg cctgagtcag gatctgttgc agctccgagt caccgggccg cggttcgtcg
      841 ccctcgtcag gttccggctc ctcgtcgcca tccccctcga cgaggtctgg ctcttcagga
      901 acgtcaacat catcgccgaa caggtcggcg agcgcctcct cgaacgtctc gcccacggca
      961 acctcctcgc caaagacgag aagcactcgc ttcaactcgg ggatcccgat cccctcggaa
     1021 atcacgaaca cgggctgcac gtacaggatc gattcttcga tcggaagcgt tatcagtgag
     1081 ccgaactgca cggccgaccc ttccctgccg aggagcgttc gggtctgcga gaactcgacg
     1141 tcctggttga tgaggttgtg gacctgtgcc ggtccgggga tgagcttctg gcgtgggaat
     1201 tgaagggata ccagctcgcc gtagtggtcg gggtccgccc gggccaccat gaacgagatc
     1261 atgttcgcct tcgtgcgcgg cgtgaacggc cgagccaaca cgaactcctc accttcttct
     1321 tgtggaagtt cgatcaagag gtaggtcggc ggcacgaggc cggactggac gctggaagaa
     1381 agtgcggtcg tctcttgcac gggttgatcg gcgaacggga tggcccacac gtcttcacgc
     1441 gagtagtagt cgttcggatc ctcgatgtgg tacgtcgtgt agacctcgga ctggatcttg
     1501 aacagatcct ccggatagcg gaagtgctcg cgcaactcca tcgacggctc ttcgtcggtg
     1561 aacaactcag ggaatgcgtt ccgccacgct gcgaccagag gatcctcatc gtcgatcacg
     1621 tagaacttca tggtcccgtc gtacgcgtcc actgtgatct tcaccgagtt gcggatgtag
     1681 ttcacgttcc ccgtcggttg acctgtgaac gagctgttaa ggatcgcccc gacgtcgaag
     1741 cgctccgagt acggatacca gggcgtggag gtgtacccgt cgaggatcca cgtcaaccgg
     1801 ccatccacgt tggcgacgta gggatcgccg tctaactgca tgaatggtgc agcgcgcaga
     1861 acacggtctc gaacgttcct gtagaagatg atcttcgact cgggagtgat gagatccgaa
     1921 aggatgaggt tcgtatcgcc ctcgcgtatc gcgaatgcga gcttcttccc gaacgttccg
     1981 accgggaccc caccttcccc ctcgtaccgg cttcgctcgg gtcccgcatc ggtggggtaa
     2041 tcgatctcct gctggttcgt gttgacgatc gagtactcac gatcttcgaa cgcctctccg
     2101 aagtagacgc gtgcttgctc gacggccaac gactcggccc cctcttcgac cgtccccgga
     2161 acgccctgca ccaggaagtc gggttgaccc gcagtggtgg cctcgttcgc gaggctcgcc
     2221 acgagtccgt agccgtgcgt gtactgaagg tgctcgttgg cccacttctt agaacggtcc
     2281 tcgagatgtt cgatcgagag ctcgcgcgcc gatagcaaaa cctgacggag ctgcccgttc
     2341 acgcgatagc gatcgatgtc cacatcgggg aactcgtaat aagggcggat cgcctggagt
     2401 tggatgtacg ccagttgcag gatgcccgga tcccacaccc gcacgttgcg gagtaggggc
     2461 tggttctgct tcagatcctc gggagtgagg tcgaacgatg cagcgaacgg acgggtttcc
     2521 acgtcgtcga gttggaacgc gccacgagtt gcctcgatgt tcctctgaag gaacgggcgt
     2581 tcgcgcggca attcgttcgg ctcgaccgag aaccgttgga cggaccacgg ccagatgccg
     2641 cctgcaacga acgagaacag gatccagatc ccgaccgcgg caagcgggag cgacacgcgg
     2701 cgtagccgga tgttcacgag gaacagcacc gccgacacga tcgagatgag agctaggaca
     2761 ttcagtgcag gcaactgggc gtgcacgtcc gtataagacg cgccgtccac cactcctcgg
     2821 gacgagaagt tcagttggaa acgccccagc cagtactggg cggacttcgt gagcgcgagc
     2881 aggccgagga tcaccgatac gtgagccaac gcaccggggg ctaccccacc caagccccgc
     2941 acggcacgga tagaaccgta cagaaggtgc gcaccgaccg ttatgagcag acaagcgaac
     3001 aacgtcgtcc agacttccga cacgagcagg tcgtacaacg gaagctcgaa cacatagaag
     3061 gagatgtcgc ggccgaactg agggtcgacc gacccgaact cgacccggtt cgcatatagg
     3121 aggaacgtct cccagttcga gctcgcgacg gcaccgatca agaaaccaac gacagccgcg
     3181 gcgccgaatc ggagcctgcg gaagtagggg gccgcggcct cccggtattt ctggagcgga
     3241 tcggagccgc cggcgatgct tatccctgcc cgataagcgg gggcgagccg ggctgccacc
     3301 acgaggttga tccacacgag agcggccacg accacgcccg tcgccagccc cactccccat
     3361 tgggtgccga ggctcgtcca caggacgctg gtgaacccga cctctttgaa ccagagaagg
     3421 tccgtgtaga agtacgcgct gaagttagaa ccgatgaaaa cgatcagaag cgtgacgacg
     3481 atgatcgcgc gccgactact tcttccacct ccagccctgg ccacgggccg agagtaatgg
     3541 caagtgcgga cctagtgtcc tggaaccgac aggacggggc tagtcgtcct ctacgacgcc
     3601 cgtcagtagg ccctcgtcct cttcctcttc ctcttcagga tcgtctgtag gggaagggga
     3661 agggatgact cgtcccgcct gacaaccgac gtcggtggga tcgcactcct cctcgccgcc
     3721 gtcgtcgtcg tcgccgccgt cgtttccgtc gtcaccttcg tcgtcaccca agccggcggg
     3781 tcggtcatca ttaccgccgg gatcctgtcc cgcatcgccg cgcgggcctg agggggccgg
     3841 gccgccgtcg gcggcctccg ccgaggcgac gctgaacacc gcttcctcac ccgaaccatc
     3901 tgccgcaacg acgcccgtgc tgaggatcac gtcttcgagc tgcgcgacga ccggacgtgc
     3961 agatacctcc atgatggcgg cggtcacgcc ccacgtcgcg cctcggtcgt agaaaccgaa
     4021 cgcgtcctta aaggcgccgg ctagaccgtg atcgcgatcg ttgttcgcga tggtgaatcc
     4081 gataagtagg tcgaccggtc tgcgcttgag gtacggacgc atgaaggaaa ctccggggcc
     4141 atctgcgaga gcgttgaagt aagcgagatt cggcttgttg ttgccgacca gacgagagaa
     4201 acctttcgct agaagcccta gctcttcctc gagctcgacg acatcttgga gcctgtccag
     4261 atcccagggg tccttctcgt ttactcgata cgggtcggat ttaccgggta gatcgccggc
     4321 cgccaccgtc gcttcgaaca agcgcgacac ggtcaatgga gactgaccgg ggctggcgag
     4381 ctcgacctgg ccctcgtagg cgccggttcg gacggtaccg aagccccgat ccacgcggaa
     4441 ggacgcctct cgtgcgcttg cagtaactcc gtcgaacgtc gcccgggtcg gttcacccgc
     4501 gtcgatcaat atgttgcccc gagtcggtcg gatcgagcgc gtactcgaga taaccatctc
     4561 cgagtgcgac tgcatcctca ggatgcgatc cccctccagc
//
LOCUS       DATMED010000061        10297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2733143, whole genome shotgun sequence.
ACCESSION   DATMED010000061 DATMED010000000
VERSION     DATMED010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733143"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VNP73_01545"
     CDS             <1..395
                     /locus_tag="VNP73_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWL64631.1"
                     /translation="TGLTPSQGWYKALEMCAHLHSWFMLQGNMGGAVKRTSEPVAYFE
                     GIVSEPAPIKKGTKLTLDAHGSYAFSNAKRLKLIPASRLRFKWNFGDGSKGKGVKVTH
                     VFKGSSTVTLTVSVGRAKDVMRQNIVIE"
     gene            complement(396..1058)
                     /locus_tag="VNP73_01550"
     CDS             complement(396..1058)
                     /locus_tag="VNP73_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HWL64632.1"
                     /translation="MPAKRAATRPIPKRSPLSRERITKAALDIADREGVDQLSMRRLA
                     ESLGAGTMTLYGHFANKGELLDAVLDLAASEKKLPGFEGTWREQIHQLVGHARDLHRR
                     HPGVVDIWSRQPALGRRGLRWPEAGMRILEGAGFATEEAALAFRLIANYTWGFALFSA
                     PRSGAGRESTQSRLAALPEDRFPHLRNAAESFAAAMGSEESFVYGLERILDGFQAKLE
                     SP"
     gene            1120..1860
                     /locus_tag="VNP73_01555"
     CDS             1120..1860
                     /locus_tag="VNP73_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64633.1"
                     /translation="MLGRTVDAIRIGVATGIVVGVVGLSFVSAAGAEPIQADDPEFSP
                     TIEWTFCDEEPIDACEPGYGAGENPRLDIVVEQDEGERDFSDLKVRFPSGYYLAPDGA
                     IADGSLLGSADVEIGSGPGCAGGEGKAPFTAEDLSVREQGRTEKEIKRGVKSVWVIEV
                     LPNSNIVLKTFGSRSRGIKIDHIIPANDFLCPPLRVEVNYQAQSEDGAAIFRNPEAPG
                     TYTFKALFVSEGDDPMLQRRAQEVTIGN"
     gene            1861..2082
                     /locus_tag="VNP73_01560"
                     /pseudo
     CDS             1861..2082
                     /locus_tag="VNP73_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004936531.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            2118..2399
                     /locus_tag="VNP73_01565"
     CDS             2118..2399
                     /locus_tag="VNP73_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64634.1"
                     /translation="MGCFAALLVLAAPRFVMVVLWLLTDYLSRAYGGFLLPLVGFFLL
                     PTTTLAYAIAQNQTGGVRGWSLVVVIIAAALDVGIWGQGRGVFARRSGS"
     gene            2448..3305
                     /locus_tag="VNP73_01570"
     CDS             2448..3305
                     /locus_tag="VNP73_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWL64635.1"
                     /translation="MSWRRTTAASIAWMLLAACGAGGAEVSDRPRVVAAFYPLEFAAQ
                     RIGGQAVDVTGLTPAGVEPHDLELASDQIRAIAAADLVVYLGDGFQPALEDALSDVDD
                     ARRLDALDAVDGSTRDPHVWLDPTLLAQITNEIAARLSEVDPDHVDLFEANAQDLSGD
                     LMDLDRELSTGLRDCEARDLVTSHEAFGHLAMRYQLHQVGISGIDPEAEPSPQRLAEV
                     ARFVEANDVRTIFFEELAPPDLAETLARETGAEIAILSPLETAPGTGDYIDTMRLNLE
                     RLRRALRCE"
     gene            3298..4035
                     /locus_tag="VNP73_01575"
     CDS             3298..4035
                     /locus_tag="VNP73_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWL64636.1"
                     /translation="MSNAAPVFEVSDAWVRLGNSDVLRGVDFAQRPGEFVVLLGPNGS
                     GKTTLVKALLGVVRLVRGRRSVFGHDLDTFRHWDRIGYVPQRVTAAAGVPATVGEVVL
                     SGRAAKVGLLGRYGSEDRAAVARALDLVDLAPLATARVETLSGGQQQRVLIARALATN
                     PEVLVMDEPVASVDLAHQESFARTLGELHRGGTSILLVAHSLGAMEPLAQRALVIERG
                     VVIHDGAPDAAAVQEHAHPHHADHEPR"
     gene            4036..4842
                     /locus_tag="VNP73_01580"
     CDS             4036..4842
                     /locus_tag="VNP73_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWL64637.1"
                     /translation="MDIFALEFMRKALLAGALVGVAAPSVGIFLVQRRLSLMGDGIGH
                     VVFMGVAAGLVLGIAPIYTALLFATGGAVTIELLRQRGKTSSDVALALIFYLGIAGGI
                     FLLGLGDTSALQLSSYLFGSVLTVSSGDLVVVAVATMATFLVTHVLRKHLFAIAYDEE
                     IARVAGLPVTSLNLLVAVLTAVTVAVTAKVVGVLLVSALLVLPVATAQQLSRSFRSTF
                     YLSVAIGALAAFAGLVIAFYTDQFPAATIVLLSIGLFVVATTIRVTRGEG"
     gene            4847..5251
                     /locus_tag="VNP73_01585"
     CDS             4847..5251
                     /locus_tag="VNP73_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HWL64638.1"
                     /translation="MVGPGPVSRATRQRAALAALMDDVDSFLSAQEVHALLRQRDAKV
                     GLTTVYRNLQAMADRGEVDMVRRADGEALYRKCATEEHHHHLVCRNCGFSVEVGSDEL
                     ERWTKKAARRHGFSDISHDLEIFGLCEACSRR"
     gene            complement(5289..6074)
                     /locus_tag="VNP73_01590"
     CDS             complement(5289..6074)
                     /locus_tag="VNP73_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWL64639.1"
                     /translation="MELGLTGKKAIVTGGSKGLGRAIAEELAREGVHLAICSRNQDEI
                     AQAGRELENHGGTVYAAVADVTDNEQVKTFVGNAVAALGGVDVLVNNAGRARPGTFQT
                     LTDQDWKEDLDVKLFSMIRCSREVLPHMRERGAGRIVNINAVQAKSPDPKFFATSTNR
                     AATLALTKTLSLELAPDNILVNSVNVGFIATPQWENIHRVRAPELSQEEFFEVMAADE
                     VPLGRFGRPEEVAGVVAFLCSDRASYMTGASIDVAGGMGRFLA"
     gene            complement(6083..6496)
                     /locus_tag="VNP73_01595"
     CDS             complement(6083..6496)
                     /locus_tag="VNP73_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWL64640.1"
                     /translation="MTDPDRYFAQQGEAHGTQGGRFVDWDALDVIEMLPGLTFQPVLG
                     DRLMVNFVRLEPNTEAPLHWHEEEQISIVIDGEFEFEVGGEKQIVRRGQAVVIPSNVP
                     HGARTYDNTCLELDVFYPPRKGLLEAMGVDRPEDG"
     gene            6560..6880
                     /locus_tag="VNP73_01600"
     CDS             6560..6880
                     /locus_tag="VNP73_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HWL64641.1"
                     /translation="MADKAQHVVLFKFPKSLTPDEEREMLEHVRRWPETIPGFTGLRL
                     GKDVGGRSGGYDYLLLTEFESEEIHQAYYSQPAHVAFSQWVAARNAEVLRVDYPLTGD
                     SLLL"
     gene            6921..7085
                     /locus_tag="VNP73_01605"
     CDS             6921..7085
                     /locus_tag="VNP73_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64642.1"
                     /translation="MTDKRTLTDEDITTTWMKAGAGSSLQADPDTTDPDGTDADGTDG
                     DSGDSDTKDS"
     gene            7102..8250
                     /locus_tag="VNP73_01610"
     CDS             7102..8250
                     /locus_tag="VNP73_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWL64643.1"
                     /translation="MNVPALERCVGDAGAFLRDHWGRAPLVHRGEGTAFEDLVTLDDL
                     DRLVSSHGLTAASLRMVRDGNTLAPSTYTVSPGTRSRGMDALISPALVYERFSAGATI
                     VLESLHRYWEPLTDLCRDLEMTIGHRLQVNAYVTPPASQGFDIHRDDHDVFVLQISGS
                     KHWSVLAPDVEDKVLIDEEIGPGTTLYIPTGCPHSAETGASASSHLTVGVLSNRASDV
                     LRAVVELAAEEPTFSERLPAAAPNASSLEDIVTRYVDEFRSWLDKVDPTDITERVARR
                     VVPSAQPIMRGQLRQLELVDSIGGDTRVRRRRGATCFLLRKEPVLKVLLADRELEMPS
                     FVEPAMREIATRDHLAVQELHSFLDEEGALVLVRRLIREGLLEVVVGT"
     gene            8240..9175
                     /locus_tag="VNP73_01615"
     CDS             8240..9175
                     /locus_tag="VNP73_01615"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HWL64644.1"
                     /translation="MARDTFRCSDASEARDEPIYGTASPVTSWLLLEQPGSWGHDALT
                     HSRLPERIGRELRSRAARAGVRVVLIRRGARLSTGHRQCYFAYTGDDATRVTHTTLRK
                     LDDLLDIDMKAIATGKPILDATEQKDPLFLVCTHGRHDACCSIRGNQVSRLACALPGV
                     DAWECSHIGGDRFAANLLCFPDGIYYGRVTPEAVTDLLHAYGNGRLSLDHYRGRSCFG
                     FAVQAAEYFVRRATDTVGLAEVKLVERPVTRPDRLVARFLIPGGRTARVEVGRDQTPA
                     AHRLTCGARSTNPIPRYELLALVIEDEAADSRPHK"
     gene            9386..>10297
                     /locus_tag="VNP73_01620"
     CDS             9386..>10297
                     /locus_tag="VNP73_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64645.1"
                     /translation="MVPPIEQGVLDRPYVITAPARARTPRLTLRERVSLTDLWAVLSL
                     VWLSRFLTGPIRFTELAFGVAAFLMISSPTARPERLTPSALDDVSRIFTRIWLAYAGI
                     CAASVAFGLGAERELLMLAVPMAPAVIGGRMLSYGIERRVLEATKKKRVLVVGSGEAA
                     ERVVETIATHDEYGLDLVGAATDGDTSEGSRLRSVTLGETSAAENLIDQFEIDVVIVA
                     SDTQGERGLSRVLRSALAGGAEVWIVPRFFELGCPSSGDHLWGLPVVRVQPPAQSRSG
                     WSWKRAMDVVVSAIVLVLISPILAAIAL"
BASE COUNT         1787 a         3138 c         3339 g         2033 t
ORIGIN      
        1 aaacgggtct cacgccctct caaggttggt acaaggctct cgagatgtgc gcacacctgc
       61 actcgtggtt catgctgcaa ggcaacatgg gcggcgcggt caagagaacg tccgagccgg
      121 tcgcgtactt cgaagggatc gtctcggaac cggccccgat caagaagggc acgaagctga
      181 ccctcgacgc gcacgggtcg tatgcgttct ccaacgcgaa gcgcctcaag ttgatcccgg
      241 cgtcccggct gcgcttcaag tggaacttcg gagatggatc gaaagggaag ggtgtgaagg
      301 tgacccacgt cttcaaagga agctccacgg tgactcttac cgtctctgtc ggcagagcga
      361 aagacgtgat gcgtcagaac atcgtgatcg agtaatcagg ggctctcgag tttcgcctgg
      421 aatccgtcca ggatgcgctc gaggccgtag acgaatgact cctcacttcc catagcggcg
      481 gcgaagcttt ccgctgcatt ccgaaggtgc ggaaacctgt cctccggaag tgcggcgagc
      541 cttgactgcg tactctctcg tcccgccccg gaacgaggcg cactgaacaa tgcgaacccc
      601 catgtgtaat tcgctatcaa acggaacgcc aaagctgctt cttcggtagc gaagccggcc
      661 ccttcgagga tgcgcatccc ggcctccggc caccgcagcc ccctcctacc gagggccggt
      721 tgtcgcgacc agatatcgac gacaccgggg tggcgccggt gcaggtcgcg cgcgtggccg
      781 actaattgat ggatctgttc gcgccacgtt ccctcgaagc ccggaagctt cttttcggag
      841 gcggcgaggt cgagaacggc atccaacaac tcccctttgt tcgcgaagtg gccgtagaga
      901 gtcatggttc ccgccccgag cgactcggca agccgacgca tgctgagctg gtcaacgccc
      961 tcgcggtcgg ctatgtcgag ggcggcctta gttatccgct cgcgggatag gggcgagcgc
     1021 ttcgggatcg gcctcgtggc ggcgcgtttg gcgggcacct ctctagagta gtacggtgta
     1081 ctagtacggt gtacggattt agggcaaagg gaggcagcga tgcttgggcg gacggtcgat
     1141 gcgattcgta taggagtagc aacggggatc gtggtcgggg tagtggggtt gtcattcgtc
     1201 tcagcggctg gagcggagcc catccaggcc gacgatccgg aattctcacc aacgatcgag
     1261 tggaccttct gcgacgagga accgatcgac gcatgtgagc cgggttacgg agcaggcgag
     1321 aacccgcgcc tcgacatcgt cgtggaacag gatgaaggag agagggactt ttcggacctg
     1381 aaggtccgat tcccgagcgg ttattacctc gctcccgatg gcgcgatagc cgacggttca
     1441 ctcctcggaa gcgcggacgt cgagatcggg tccggcccgg gctgcgccgg aggcgaaggc
     1501 aaggctccgt tcacggccga ggatctctcc gtgagggaac agggtcgcac cgagaaggag
     1561 ataaagcgcg gcgtcaaatc ggtatgggtc atcgaggtat tgcccaactc caacatcgtg
     1621 ctgaagacct tcggaagtcg aagtcggggc atcaagatcg accacatcat tcctgccaat
     1681 gacttcctct gcccacctct gcgggtggag gtcaactatc aggcccagtc cgaagacggg
     1741 gcggcgatct tccgtaatcc ggaagcgccg gggacgtaca cgttcaaggc actcttcgtc
     1801 agtgagggcg acgacccgat gctccagaga cgcgcccaag aggtcacgat cggcaattag
     1861 aacgacctcc agcggccgat aggggcgtat ctctacggcc ggcgcttata cgaagtcatg
     1921 gttgcgtggg agacgatgtc agtcgacggc gagccgcctt ccatccgtga ttacacccag
     1981 atatggcagg cagccgacaa gatcgtgtac tcgaagacgc tcgagtcggt aacgagcggt
     2041 aaaacgcgca tcgagcggga cttcgacacc gacgtggtcc ggaacggtgt tgtctatctc
     2101 cgctaccgtg tcgactgatg ggttgtttcg ctgcacttct ggttctcgct gcacctcgct
     2161 tcgtgatggt cgtcctgtgg cttttgaccg attacttgag ccgcgcttac ggaggattcc
     2221 tgttacctct cgtgggcttc tttcttctcc ccacgacgac cctcgcttac gcgatcgccc
     2281 agaaccagac cggcggggtg cgcggatggt cgttggtggt cgtcatcatc gcggcggccc
     2341 tggatgtagg gatatggggg caggggaggg gcgtcttcgc gcggcgatcg ggttcatgat
     2401 cgaacgactt gttgacaacg gttttcatta gcgtttagtt tcctgccatg tcgtggcgcc
     2461 gtacgacggc ggcatccatc gcgtggatgc tgctcgccgc ctgtggggcc gggggggccg
     2521 aggtatcgga tcgccctcgg gtggtggccg cgttctaccc gctcgaattc gccgcgcagc
     2581 ggatcggggg gcaggccgtc gacgtcaccg ggctcacacc cgccggtgtc gagccccacg
     2641 atctcgagct ggcgtcggat cagatccggg ccatcgccgc cgccgatctc gtggtgtacc
     2701 tcggggatgg attccagccg gcgctggagg acgcgttgtc agacgtcgac gacgcccgcc
     2761 gcttagacgc gctcgacgca gtggacggtt ccacccggga tccacacgtg tggctggacc
     2821 cgacgctcct cgcacagata accaatgaga tcgctgcgcg cctatcggag gtcgacccgg
     2881 atcacgtcga cctcttcgag gcgaatgcgc aggacctgtc cggcgatctc atggacctgg
     2941 atcgggagct gtcgaccggc cttcgcgatt gcgaggcccg agacctcgtc accagccacg
     3001 aggccttcgg tcatctggcg atgcgttatc aactgcacca agtgggcatc tcggggatcg
     3061 atcccgaggc cgagccttct ccgcaacgtc tcgccgaagt ggctcgcttc gtcgaagcga
     3121 atgacgtccg cacgatcttc ttcgaagaac ttgctccacc cgatcttgcc gagacgttgg
     3181 cgcgcgagac gggcgccgag atagccatct tgtcgccact tgaaaccgcg ccgggaaccg
     3241 gtgactacat agacaccatg cggctgaatc tcgagcgact ccgaagggcc ctccgctgtg
     3301 agtaacgcgg cgcccgtctt cgaggtgagt gacgcctggg tgcgcctggg caattccgat
     3361 gtccttcgcg gtgtcgattt cgctcaacga ccgggagagt tcgtcgtgtt gctggggccg
     3421 aacgggtccg gcaagaccac actcgtcaag gcgctactgg gggtcgttcg actggttcgg
     3481 ggccggcgat cggtgttcgg tcacgacctc gacaccttca ggcactggga tcgcatcggc
     3541 tacgtcccgc agcgcgtgac cgcggcggca ggtgtcccgg ccacggtcgg cgaggtcgtg
     3601 ctatcgggtc gcgcggccaa ggtgggcctg ctcggtcgtt atggaagcga ggatcgagcg
     3661 gcagtggccc gagcgctgga cctcgtcgac ctcgcgccgc tggcaacggc acgggtcgag
     3721 accctctccg gcggccaaca gcaacgggta ctgatcgctc gcgctctcgc aacgaacccg
     3781 gaagtgctcg tcatggacga accggttgcg agcgtcgacc ttgctcacca ggaaagcttc
     3841 gcccgcacac tgggtgagct tcaccggggc gggacctcga tcctgctggt cgcccattcg
     3901 ctgggagcca tggagcccct agcgcagcga gcgctggtga tcgagcgggg ggtcgtgatc
     3961 cacgacggcg cccccgacgc cgccgcggtc caggaacatg cccacccaca ccacgccgat
     4021 cacgagccca gatagatgga catcttcgct ctcgagttca tgcggaaggc actcctcgcc
     4081 ggggctctag tgggggtcgc ggcgccgtcc gtcgggatct ttctggtcca gcgccggctg
     4141 tccctgatgg gggacggcat cggacacgtc gttttcatgg gtgtcgccgc cggactcgtg
     4201 ctcggcatcg ctccgatcta caccgcattg ctgttcgcga ccgggggtgc agtcacgatc
     4261 gagctgttgc gtcagcgtgg gaagacctcg agtgatgtcg cgctggcgtt gatcttctat
     4321 ctcggtatcg caggagggat cttcttgctg gggttggggg acacctcggc actccaactc
     4381 agtagctatc tgttcggctc cgtgctgacg gtgagttctg gtgatctggt ggtcgtcgcg
     4441 gtcgcaacga tggcgacttt ccttgtcacg cacgttctgc gcaagcacct gttcgcgatc
     4501 gcttatgacg aagagatagc gcgcgttgcc ggtctcccgg tcacctctct gaaccttctg
     4561 gtcgctgtgc tgacagccgt aaccgtcgcc gtcacggcga aggtggttgg tgttcttcta
     4621 gtgtccgcgc ttcttgtgtt gccggtagcg accgctcaac agctatcccg gagcttccgt
     4681 tccaccttct atctcagtgt tgccatcgga gcattagcgg cgttcgccgg tttggtgatc
     4741 gcgttctata ccgatcagtt cccggcggcg acgatcgttc tcttgtcgat cgggttattc
     4801 gtggttgcca caacgatccg cgttacacgt ggggaggggt agagccatgg tgggacctgg
     4861 acccgtctct cgagcaacgc gccaacgagc tgcccttgct gcgctgatgg acgacgtcga
     4921 ttcgttcctc agtgctcagg aggtgcatgc cctgctacga caacgtgacg ccaaggtggg
     4981 cctgaccacc gtttaccgga acctccaggc gatggccgac aggggagagg tggacatggt
     5041 gcgccgcgcc gacggcgaag ctctctatcg gaagtgtgcg accgaagagc atcaccacca
     5101 cctcgtgtgt aggaactgtg ggttctcggt ggaggtggga agcgacgaac tggagcggtg
     5161 gaccaaaaag gcggcccgtc ggcacggctt ctccgacatc agtcacgatc tcgagatctt
     5221 cggcctgtgc gaggcgtgtt cgaggcgatg agccggccga tgtggaccgg ccctgaatag
     5281 ctgaacgctc aggcgaggaa acggcccatc ccaccggcga cgtcgatgct ggcaccggtc
     5341 atgtagctgg cccgatcgct gcacaggaag gccactactc cggcgacctc ttccggcctg
     5401 ccgaaccgcc ccaatgggac ctcatcggcc gccatcacct cgaagaactc ctcttgtgac
     5461 agctcgggtg cgcgcaccct gtggatgttc tcccattgag gtgtcgcgat gaaaccgacg
     5521 ttcacgctgt tgaccaagat gttgtcggga gccagctcga gagaaagagt tttcgtcaag
     5581 gcgagcgtcg cagcgcggtt cgtcgaggta gcgaagaact tggggtcggg cgacttcgct
     5641 tgcacggcgt tgatgttgac gatccgcccc gctccgcgct cgcgcatgtg gggaaggacc
     5701 tcgcgcgagc accggatcat cgagaagagc ttgacgtcca ggtcctcttt ccagtcttga
     5761 tcggtcagcg tctggaaggt gccgggccta gctcgccccg cgttgttgac gaggacgtct
     5821 accccaccca gagccgccac cgcgttaccc acgaacgtct tcacctgctc gttatcggtt
     5881 acgtcggcta ccgcggcgta gaccgtgccc ccgtggttct cgagttcccg gccggcctgg
     5941 gcgatctcgt cttggttccg agagcagatg gcgaggtgga cgccctcgcg cgccaactcc
     6001 tctgcgatcg cccggcccag acccttcgat cctcccgtga ctatcgcttt cttgccggta
     6061 agccccaact ccatccgggc tcctatccgt cctcgggccg gtccacgccc atcgcttcga
     6121 gcagcccttt tctcggcggg tagaacacgt ctaactcgag acaggtgttg tcgtaggtgc
     6181 gcgccccgtg cgggacgttc gacgggatga cgactgcctg gcctctgcga acgatctgct
     6241 tctcgcctcc gacctcgaat tcgaactccc cgtcgatgac gatcgagatc tgttcctctt
     6301 cgtgccagtg caagggggct tccgtattcg gctcgaggcg cacgaaattg accatcaggc
     6361 ggtctccgag aaccggctgg aaggtaagac ccggcagcat ctcgatcacg tcgagtgcgt
     6421 cccagtcgac gaatctgccg ccctgggtcc cgtgagcctc gccttgttgg gcgaaatacc
     6481 tgtccggatc cgtcatcggt cctcctcgcc ggtggccaag cctggagaat atgggtcttc
     6541 gaacgagcga ggaggtcgag tggccgacaa agcgcaacac gtcgtcctgt tcaagtttcc
     6601 gaagtccctc acccccgatg aggagcgcga gatgctcgag cacgtgcgga ggtggcccga
     6661 gacgatcccg ggattcaccg gcctgcggct cggtaaggac gtggggggcc gttccggcgg
     6721 atacgactac ctgctgttga ccgagttcga gagtgaggag attcatcagg cctattactc
     6781 gcagcccgct cacgtagcgt tcagccagtg ggtggccgct cgaaatgcgg aggttctccg
     6841 tgtcgattac ccgctgaccg gggattccct cctgctttag ggttcacccg actggatgtc
     6901 ggatggggag gtgcttctta gtgacggaca agaggacgct cacggacgag gacatcacaa
     6961 cgacctggat gaaggccggt gccggttcgt ctctccaggc agatcccgac accaccgacc
     7021 cagacggcac ggacgccgac gggaccgacg gcgactcggg cgactccgac acgaaagaca
     7081 gctagtcatc cacctccatc aatgaacgtc ccggcgctcg aacgatgcgt cggggatgcg
     7141 ggggcgttcc tgcgcgacca ctggggccgg gccccactgg tccatcgagg tgagggaacg
     7201 gccttcgagg acctcgtcac gctcgatgac ctggacaggt tggtctcgtc gcatggcctg
     7261 accgcggcga gtctccggat ggttagggac gggaacacgt tggctccgtc gacctacacg
     7321 gtgtcgccgg ggacgagatc ccggggaatg gatgcgctga tcagtcccgc gcttgtttac
     7381 gagcgattct cggccggggc aacgatcgtt ctcgagtcgc tgcatcgcta ttgggagccg
     7441 ttgaccgatc tctgtcgcga tctcgagatg accatcggcc accgtctgca agtgaatgcc
     7501 tacgtgactc caccggcatc gcaaggtttc gacatccatc gtgacgacca cgacgtgttc
     7561 gtcctacaga tctcgggatc gaagcactgg agcgtcctcg cccccgacgt cgaagacaag
     7621 gttttgatcg acgaagagat cggtcccggc acgacgctct atatccccac gggctgccca
     7681 cattcggccg agaccggtgc gagcgcgtcg tcgcacttga cggttggtgt cctatccaat
     7741 cgagcgtcgg atgttctgcg ggccgtcgtt gaattggccg cagaggaacc cacattcagt
     7801 gagcgacttc ccgccgcggc accgaacgca tcctcgctcg aagacatcgt gacccgatac
     7861 gtcgatgagt ttcgttcgtg gctcgacaaa gtcgacccca cagacatcac cgagcgcgtt
     7921 gcgaggaggg tcgtgcccag cgcacaaccg atcatgcgag gacaactgcg acagctggag
     7981 ctcgtggatt cgatcggcgg cgacacaagg gttcgacgga ggcggggcgc gacctgcttc
     8041 ctgctgagga aagagccggt cctcaaggtt ctgctggccg atcgcgagct cgagatgccg
     8101 tcgttcgtcg agcctgccat gcgtgagatc gcaactcgcg atcacctcgc cgtgcaggag
     8161 ctgcactcgt tcctggacga ggaaggagcg ctcgtgctgg tgcgtcggtt gatccgggag
     8221 gggttgctcg aggtcgtcgt tggcacgtga cacgttccgc tgctccgatg cgtccgaggc
     8281 gcgagacgaa ccgatctacg ggacggcatc gccggtaacc agctggctct tgctggaaca
     8341 gcccggatcg tggggacatg atgccttgac gcacagccga cttcccgagc gcataggaag
     8401 ggagctccgt agccgcgccg ctagggcagg ggttcgggta gtccttatcc gtcgcggcgc
     8461 ccggttgtcg acggggcacc ggcagtgcta cttcgcgtac acgggcgacg atgccacccg
     8521 cgtgacgcac acgacactga ggaaacttga cgacctcctg gacatcgata tgaaagcgat
     8581 cgctacgggc aagcccatct tggatgcgac cgaacaaaag gatcctctct tcctcgtctg
     8641 tacccacggg cggcacgatg cttgctgttc gatccgtggg aatcaagtct cccggctcgc
     8701 gtgcgcgctg ccgggggttg atgcgtggga gtgctctcac atcgggggcg atcgattcgc
     8761 tgcgaacctt ctgtgtttcc cggatgggat ctattacggg cgcgttactc ccgaggcggt
     8821 gactgatctt ctccacgcct atggcaacgg aaggttgtcg ctggatcact atcgcggccg
     8881 tagctgcttc ggattcgccg tccaagctgc cgagtatttc gttaggcggg caacggacac
     8941 cgtcgggctc gcggaagtga aactcgtgga acgacccgtc acgcggccgg accgtctcgt
     9001 cgctcgcttc ctgattccgg gtgggcgcac ggcgcgggtg gaggtgggcc gtgatcaaac
     9061 gccggccgcc caccggctca cttgcggcgc gcggtccacg aacccgatcc cgcgctacga
     9121 actccttgct ctggtgatcg aagacgaggc ggcggattca cgaccacaca aatgatcgtc
     9181 gcgaaaatat ggcccggcta cggggggttt ccgaacgatc acgaaggggc atatttaggg
     9241 cgtggtgggg gtccagcttc taatagttgg caattcggca tgaattcgca ggacacgggg
     9301 tatttgccct gaatacgtag gtctgagcgg gtttaatgct ccactggcgg taaggcggat
     9361 ttggcgggct tcagagggag gcggagtggt tccacccatc gagcagggcg tgctcgacag
     9421 gccctacgta ataactgcgc ctgcgcgcgc ccgaactcca cgattgaccc tgcgcgagcg
     9481 ggtttcactg actgatctgt gggcggttct cagcctggtg tggctgtcgc gtttcctcac
     9541 cggtccgatt cgtttcaccg aacttgcctt cggggtcgct gcgttcctga tgatctcctc
     9601 tcccaccgcc cgccccgagc ggctcactcc gagtgcactg gatgatgttt cgaggatctt
     9661 cacgcgcatc tggctcgcct atgcagggat ctgcgcggcg tcggtcgcct ttggcctggg
     9721 ggccgagaga gagcttctga tgctcgcagt gcccatggcc cctgcagtga tcggcggccg
     9781 gatgctgtcg tatgggatcg agcggcgcgt cctcgaagcc accaagaaga aaagggtcct
     9841 tgtcgtcgga tcgggagagg cagcggaacg tgtggtggag acgatcgcca cccacgacga
     9901 gtacgggttg gaccttgtgg gggccgcgac ggatggcgac acatccgagg ggtcgcgcct
     9961 tcggtccgtg accttgggtg agacctccgc tgcagagaac ctcatcgatc agttcgagat
    10021 cgacgttgtg atcgtggctt cggacactca gggcgagcgc ggtttgtcga gagttcttcg
    10081 ctccgcgctg gcgggtggag ccgaggtgtg gatagttcct cgtttcttcg agctcggttg
    10141 tccgtcttcg ggcgaccacc tgtggggact gccggtcgtg cgcgtacagc cacctgcgca
    10201 gagccggtcg ggatggtcgt ggaagcgtgc gatggatgtc gtcgtttctg caatcgttct
    10261 ggttcttatc tccccgatcc tggccgcgat cgccctg
//
LOCUS       DATMED010000062         3289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2354644, whole genome shotgun sequence.
ACCESSION   DATMED010000062 DATMED010000000
VERSION     DATMED010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354644"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VNP73_01625"
     CDS             complement(<1..898)
                     /locus_tag="VNP73_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWL64646.1"
                     /translation="MSFHNPVHRMDRALGETLERGLRAHHRRRLSKIGWKQVFDPHRG
                     AALGNYLARDGNALKVHVDGEDSFKAIAAAVRAARSHVHLAGWHMEAGFVLLAEPEEL
                     ALKDLLAESGARTEVRVLVWGGAPLPPPFRPRRSEAKELREQLAHLSGVTIALDSKER
                     LLHCHHEKIVVIDDEVAFVGGLDFSTIGASRLDGSDHPPREPMGWHDATVEMRGPIVA
                     DVADHFRMRWKEVTGRSLSRAPAPAPAGDVEAQIVRTVPEKVYDSLPNGDFGILGAYV
                     DALQDAREFIYLENQFLWSSEIV"
     gene            956..1459
                     /locus_tag="VNP73_01630"
     CDS             956..1459
                     /locus_tag="VNP73_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWL64647.1"
                     /translation="MKGTPEERQVQDEFLREKTESFFGYVEDLFYISVAVALTLAGAG
                     LFGYVVYDFVSKIGDSEFSVTILDFLDGLLLVFIITELIHTIRAVIDEKVLVAEPFLI
                     VGIVAAIRRLVVISAEAKDLLGTPDFADAMLEIGILTGTIVFIAVALFLLRSNVRSEP
                     SSAHEPD"
     gene            1710..>3289
                     /locus_tag="VNP73_01635"
     CDS             1710..>3289
                     /locus_tag="VNP73_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWL64648.1"
                     /translation="MPKEMESHIDLQPVRFSMDRRRFIAATGTLVGGTALGVTTGAAP
                     ALAAKVISGHKSFEHLNIGPNEVVVFNPNKDTTVHAHMVHVEGVLRMRPAKPGVKHRL
                     IIGEGLHVEGGGRLDIVGTKKKAWTRARSGLKSGQRRIKVTSVDGWRAGDRVVITPTG
                     SPRNPSFDNAYDSGRIRRIDRRRKIITLSSPLRRDHPRVNVGVMPGERKARRYGAEVL
                     NMSRNVQIDGSGDVHIHNTRRAKHRVNYANISGLGVRDELGRYPLHFHTCRNNAAGSI
                     VRGTVVENSANHGFVAHSSNRITFSDCIAHNIRDSAFWWDRQDESGDKESNFVTYRRC
                     VASDVDFDETPQAFKIMGFFLAAGRDRSNSCIGCVAVGIKGQDNANGFGWDADAQSVW
                     NFRDCLAHNNRRNGIWVWQNTSRIHRISKTVCYHNGDVGIDHGSYANNYHYDGIICHG
                     NKNAQVLVLASSRGRQTFRNVLMDGTGLSEFAMIVSTGVPTPDAVDDNLFTTIENCVF
                     RNYRRKGVQMTEAHENTQ"
BASE COUNT          655 a         1029 c          979 g          626 t
ORIGIN      
        1 tcacgatctc cgatgaccac aggaactggt tctcgaggta gatgaattcc cgagcgtctt
       61 gaagcgcgtc tacgtaggcg ccgaggattc cgaagtctcc gttcgggagc gagtcataga
      121 ccttctcagg caccgtgcga acgatctggg cctcgacgtc accggcggga gcgggcgcgg
      181 gcgctcgact gagagatcga ccggttacct ccttccacct catacggaag tgatccgcga
      241 cgtccgcaac gatcggcccc ctcatctcga cggtcgcgtc gtgccagccc atcggttcgc
      301 gaggcgggtg atcggaaccg tccagccggc tggctccgat ggtcgagaag tcgaggcctc
      361 ctacgaacgc cacttcgtcg tcgatcacga cgatcttctc gtgatgacag tggagcaggc
      421 gctctttcga atccagtgcg atggtcacac cactgagatg ggccagttgc tcgcggagct
      481 ccttggcctc acttcgtcgc ggtctgaacg gtggtggaag cggcgcaccg ccccatacga
      541 gcacgcgcac ctccgtcctc gctccagact ctgcgagcag gtccttgagg gcgagctcct
      601 caggctccgc gagcagcacg aacccggcct ccatatgcca gccggcgaga tgcacgtgcg
      661 accttgctgc acgaacggcg gccgcgatgg ccttgaaaga gtcctcaccg tcgacgtgaa
      721 ccttgagagc gttgccgtcg cgcgctaggt agtttccgag cgccgcccct cgatgggggt
      781 cgaagacctg cttccatcca attttgctga gccggcgacg gtggtgggca cgcaggcccc
      841 gctccagggt ctcaccgagg gctcggtcca tccgatgcac ggggttgtgg aaactcatga
      901 cacagtctta cccctcaagc gtcacgcgcc tcccggaccg tatagttttc ccaggatgaa
      961 gggcacccca gaggagcgac aagtacagga tgagttcctg cgcgagaaga ccgagagctt
     1021 cttcggctac gtcgaggacc tcttctacat ctccgtcgcg gtcgccctga ccctcgccgg
     1081 tgccggcctc tttggctacg tcgtttacga cttcgtatcg aagatcggcg actcagagtt
     1141 ctcggtcacc atcctcgatt tccttgacgg gcttctgctc gtattcatca tcaccgagct
     1201 catacacacc attcgcgccg tgatcgacga gaaggtgctc gtagcagaac cgtttctgat
     1261 cgtggggatt gtggctgcaa tccgcaggct cgtcgtcatc agtgccgagg ccaaggacct
     1321 gctggggacg cccgacttcg ccgacgccat gctcgagatc gggattctca ccggcacgat
     1381 cgtgttcatc gcggtggcgc tcttcctcct gaggagcaat gtccgcagcg agcccagctc
     1441 cgcacacgag cccgactgac ttatgggtgg gtttcgtccc attcgcaaga gtcgttgact
     1501 gtcttttctg tccccgacgg caccagtaac cccagagggc cggccgtttc cggacaaacc
     1561 ctggcaccga ggacaacctt aggatgggat ggttgacttt caggctttat gggcaagcat
     1621 tgcaagcaat cgcgggattc acaggggcct tctcgaaggc gtctgcgcgc tcgtagagcg
     1681 cacatcaaga ctcaaaggag cggcccgtta tgccgaagga gatggagtcc cacatcgact
     1741 tgcagcccgt caggttttca atggatcgca gacgcttcat cgctgcaacc ggcacgctcg
     1801 tcggggggac cgctctcgga gttacgaccg gcgccgctcc ggcgctggcg gccaaggtaa
     1861 tctccggcca caagagcttc gaacacctca acatcggtcc caacgaggtc gtggtcttca
     1921 accccaacaa ggacaccacc gtccacgctc acatggtcca cgtcgaaggt gtgttgcgca
     1981 tgcgcccggc caagcctgga gtcaagcacc gtttgatcat tggcgagggc ctccacgttg
     2041 aaggtggagg tcggctcgat atcgtgggaa cgaagaagaa ggcctggact agagcacgtt
     2101 cgggtctcaa gtcgggacag cgccggatca aggtgaccag cgtcgatggc tggcgagccg
     2161 gcgaccgagt cgtgatcaca ccgaccgggt cgccccggaa cccgtcgttc gacaacgctt
     2221 acgactccgg caggatcagg cgcatcgatc ggcgtcgcaa gatcatcacg ctgagctcgc
     2281 cgctcaggcg tgaccatccc cgcgtcaacg tcggcgtcat gcctggagaa aggaaggctc
     2341 gtcgctacgg cgccgaggtc ctgaacatgt cgcgcaacgt ccagatcgac ggctcagggg
     2401 acgtacacat tcacaacacc aggcgggcga agcatcgcgt caactacgcc aacatctcgg
     2461 gtctcggcgt tagggacgag ttgggccgct accctctgca cttccatacc tgccgcaata
     2521 acgcggcggg ttcgatcgtt cgaggcaccg tcgtggagaa ctccgccaat cacggctttg
     2581 tggctcactc atcgaaccgc atcaccttct ctgactgcat tgcgcacaac attcgcgact
     2641 ccgccttctg gtgggatcgg caagacgagt cgggcgacaa ggaatcgaac ttcgttacct
     2701 accggcgctg tgtcgcatcg gacgttgact ttgacgagac tcctcaggcg ttcaagatca
     2761 tggggttctt cctggccgcc ggccgagaca gatcgaacag ctgtatcggg tgcgtcgccg
     2821 tggggatcaa gggtcaggac aatgccaacg ggttcggctg ggacgccgac gcacaatcgg
     2881 tatggaactt cagagactgc cttgcacaca acaacaggcg caacggtatc tgggtgtggc
     2941 aaaacacgag ccggatccac cgcatctcga agaccgtttg ctaccacaac ggtgacgtcg
     3001 gcatcgatca cgggtcgtat gccaacaact atcactacga cgggatcatc tgtcacggca
     3061 acaagaacgc gcaggtgctc gttctggcgt catcgcgcgg taggcagact ttccgcaacg
     3121 tcctgatgga tgggaccggt ctgagcgagt tcgccatgat cgtctccacc ggagtaccga
     3181 caccagacgc ggtagacgac aacctcttca cgacgataga gaactgtgtg tttcggaact
     3241 atcgtcgcaa gggcgttcag atgaccgaag cgcacgagaa cacgcagtt
//
LOCUS       DATMED010000063         8576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4656099, whole genome shotgun sequence.
ACCESSION   DATMED010000063 DATMED010000000
VERSION     DATMED010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4656099"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VNP73_01640"
     CDS             complement(<1..1334)
                     /locus_tag="VNP73_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWL64649.1"
                     /translation="MPVEGWGVFVSFLGAIGGLGVYSGAAGPIGRFFAYLATLFVGSV
                     AILLPPLAIATGVMIIVPRLREQLIRILIGTGLCLLAVTAMVHLAQGNKKISAKIETL
                     QEIGGIFGALGARPLADVIGIWPSWLVLVLVFGVGLMVITRTPVSRVAGWVKAGGLVA
                     FDFVRGLADRIEVDEDEDEDDESDYSEDGHEETYDSYEGDAMQPDVPEGEPDPAGDEL
                     PTASGDEAPKQMAMPVGNTKSYRLPPLELLSKGGEREISSRSIDETTRALEDTMRQFK
                     VDARVTGYTAGPQVTRFEIELGEGVKVNSVLSLSNEIKYALASGELRFLAPIPGRSAI
                     GVEVPNRTRQLVTLGDVLRSKTAQKDMHPLSVSLGTDISGESVMANITEMPHVLIAGA
                     TNSGKSSCLNSMITSILVRSRPDQVRMILIDPKRVELNHFSAVPHLLTPVVTV"
     gene            1611..2081
                     /locus_tag="VNP73_01645"
     CDS             1611..2081
                     /locus_tag="VNP73_01645"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HWL64650.1"
                     /translation="MRAIAPACVVLALALVACDSPGSAPPAGLPSARETSSDPSPEER
                     TTPPKSVPESKRAPIEVTSPAQGDEVTSPLIVTGTANVFEATVSYQLVDGSGNEITKG
                     FTTASCGTGCRGDYSKKIRFTVDEPTEATLEVFESSAEDGTALHVVRVSLTLLP"
     gene            complement(2078..2560)
                     /locus_tag="VNP73_01650"
     CDS             complement(2078..2560)
                     /locus_tag="VNP73_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64651.1"
                     /translation="MKRALICLLTFVALASVVPVAAQDESQPPDLVFRSGDTRQRGNL
                     GSYCWESPSGSKCEDTFSFDWPPPDQAKSRAKARVVLKYPEKPKQMSLDYWRRVNENG
                     EPAGRPHGIEFRLEPRDGRRGRVWDAKFRLPSSGRFYMRAFAQWDAGDGFYDFNIVLD
                     "
     gene            complement(2557..4212)
                     /locus_tag="VNP73_01655"
     CDS             complement(2557..4212)
                     /locus_tag="VNP73_01655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWL64652.1"
                     /translation="MTAAPTRVIFLGGLGEIGRNMIALESEGKILVIDAGLSFPNEEM
                     LGVDLVLPDFSYLRDRAADCLAVVLTHGHEDHVGALNWLMRDIDMPVYGTPLTLGLAR
                     KRLDEFHTKAELNPISAPGELKLGPFACRFLPVSHSIPDALAVLTRTPGGTILYTGDF
                     KLDHSPIDGRSTDLEAFAAAGREGVDLLLSDSTNAEEEGRTPSESLVGPRLKEIFAAA
                     QRRVVVACFASNLHRIQQVSDAAEATGRKVAFVGRSMVNNVDVGRELGLLSISDGTLM
                     DVGEMNDVDPSRSVVVCTGSQGEPLSALTLIASGEHKLVSVERDDTVILSASPIPGNE
                     SAVHRVVNALYKAGAEVYKGEDDGVHVSGHAASDELAEMIRTVGPKNFCPVHGEYRHL
                     AKHARIAEQQGVASDDIWMVEDGDVLQLVSGRVKRGDRVRAGMVLVDGLGVGDVGPVV
                     LRDRRVLSEDGIVMCVVTIDSQNGEILAGPDLISRGFVHEQEQRDLLDEGADRVADAL
                     EKLERDQVTDWGVIKKECRKALGEFVWQRTRRRPMILPVIMEV"
     gene            complement(4209..5105)
                     /gene="dapA"
                     /locus_tag="VNP73_01660"
     CDS             complement(4209..5105)
                     /gene="dapA"
                     /locus_tag="VNP73_01660"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWL64653.1"
                     /translation="MSARFGEVMTAMVTPFKKDFALDVEAVPPLVDHLIEHGSEGLIV
                     CGTTGESPTLSHDEKLTLFETVAQSAGDRAKVIAGTGTNNTAESITLTKEACELGVDA
                     CLLVTPYYSKPPQSGLLAHFRAIADASTAPVIVYDIPGRTGRRIERSTMIELARHERI
                     VGDKDAVGDAGETARLRSELDAEGLQDFELISGDDALVLPHIGAGVTSIISVCSHLVG
                     PQIKQIFSAYSDGKVDEARRIFLELQPLFQTIMGGASPIPVKAALNMIGVEVGPPRLP
                     LVEATDAEKKVIRAALESSGLV"
     gene            complement(5142..5630)
                     /locus_tag="VNP73_01665"
     CDS             complement(5142..5630)
                     /locus_tag="VNP73_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64654.1"
                     /translation="MRRTVALALLALVAAACVGEPDPNLTESGKGKPQVTALMPNEAE
                     PGSTVTAAVRIANPGPGDMESVVVAFSRIGNAGLPAPIVDVGTGGRTDGVKSVRPEPV
                     AVSQDAVVYRFGGLPEGEALEIRFELVLPENEEGEVGNAILVYDGAEPDRAKGTRLST
                     FL"
     gene            complement(5627..6352)
                     /gene="dapB"
                     /locus_tag="VNP73_01670"
     CDS             complement(5627..6352)
                     /gene="dapB"
                     /locus_tag="VNP73_01670"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWL64655.1"
                     /translation="MKVGVLGAKGRMGATVCAAVEAETDLELVARIDQDEPLEDLLAN
                     GAEVAVDFTTPDVVKDNIRFCLENEIHVVVGTTGFEESDFDEVRKWAEAGPANAFIAP
                     NFALGAVLMMKFAAEAAAFFSNAEIIERHQEKKLDAPSGTAQRTAHLMNEARGRAWDE
                     VTRGRGEEFRGGDESGLRIHSVRVPGSVAHQEVILGNAGETLTIRHDSIDRASFMPGV
                     VLAVKKVASLDGCVVGLEHLLDL"
     gene            complement(6357..7727)
                     /locus_tag="VNP73_01675"
     CDS             complement(6357..7727)
                     /locus_tag="VNP73_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWL64656.1"
                     /translation="MIATEEDHAATAVARAVKAAEVGDRVEREPLAATTVKAMTRFAR
                     TVLDSGATVVTEEMTEVRSVSLGFWFNIGSRDEPAELAGTSHFLEHLLFKGTPRRSAQ
                     DIADAFDAVGGDVNAFTGKEYTCYYSRVLDTDLPMAFDVLSDMISASLIDPKEFESER
                     KVILEEIAMHEDAPDELVHDLFYRYLWDGHPLGRPVLGFNETIGAVSRDEVASYWQNR
                     YGPLNLVLAAAGSVKHDVLVEKVAGLLGDRDGARTGREIESPKPRAGVKVYRRPTEQA
                     HIVVGTEALPRNHPDRHALAVLDTILGGGMSSRLFQEVREKRGLAYSVYSYRSLFADT
                     GSFAVYAGTTPQNADDVISIVHDEIQKVVDEGVTDAELARAKGHVKGALVLSSEDPGS
                     RMNRLGRQQITTGEILSIDELIEKFEALEMDDLRRVAANVLGTGSFHVSVVGPFDENA
                     FDQYAA"
     gene            complement(7618..>8576)
                     /locus_tag="VNP73_01680"
     CDS             complement(7618..>8576)
                     /locus_tag="VNP73_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWL64657.1"
                     /translation="GSSLAMMDAGVPIRGGKHVGGVAMGLIAEDGKFQPLTDILGDED
                     AFGDMDFKVAGTEDVVTALQLDTKISGVPAEVLRDALMAAKKARLHIIGEMNKTIDHP
                     HEELATFAPRVLAVKIPVDKIGEIIGPKGKNINAITAQTGVEIDIDEDGTVRIGSTDQ
                     ESAEAAAKMIEETANPRMPEMGERINGQVVKTTTFGAFVNISPGRDGLVHISKLGEGR
                     IERVEDAVNAGDQLEVEVTDLDRQGRISLTPVAWLERKVAEGMSIEEAREAAKSSSGG
                     SRGGGGRDRDRGGPRRDRGGPRREGGGGGGPRRERAPRRDDS"
BASE COUNT         1526 a         2900 c         2584 g         1566 t
ORIGIN      
        1 accgtcacaa cgggcgtcag cagatgtggg acggcggaga agtgattgag ctcgacgcgc
       61 ttcggatcga tcaggatcat gcgcacttga tcgggacgag agcgcacgag gatcgaagtg
      121 atcatcgagt tgaggcaaga ggacttaccc gagttcgtgg ctccggcgat caggacgtgc
      181 ggcatctcgg tgatattcgc catcaccgac tcgccggata tgtcggtccc aagggatacc
      241 gaaagcgggt gcatgtcctt ctgggccgtc ttggaacgca agacgtcccc gagggtcacg
      301 agctgccggg tgcggttcgg gacctccacc ccgatggcgc tgcgcccggg gatgggggcc
      361 aggaacctca attcccccga cgccagggcg tacttgatct cgttcgagag cgagagcacg
      421 ctgttcacct tcacgccttc gccgagctcg atctcgaatc tcgtgacctg cggccccgcc
      481 gtatacccgg tgactcgagc atccaccttg aactgacgca tcgtgtcctc cagcgcccgc
      541 gtcgtctcgt cgatagaacg agacgagatc tcacgctcac cccccttgga cagaagctcg
      601 agcggcggca accgataaga cttcgtgttt ccaaccggca tggccatctg cttcggcgcc
      661 tcgtctccag acgcagtcgg aagctcgtca ccggccgggt cgggttcgcc ctcgggcaca
      721 tccggttgca tggcgtcgcc ctcgtaagag tcgtaggtct cttcgtggcc gtcctcggag
      781 tagtcgctct cgtcgtcctc gtcctcgtcc tcatcgacct cgatgcgatc ggccaagccg
      841 cgcacgaaat cgaaagccac gagacccccg gcctttaccc atcccgccac cctgctgacc
      901 ggcgtacggg tgatgaccat gagtccgaca ccgaacacga ggaccagaac cagccaactg
      961 ggccagatac cgatgacatc cgcgaggggg cgcgcaccca acgctccgaa tatcccgccg
     1021 atctcttgca gagtctcgat cttggccgag atcttcttgt tgccctgcgc gaggtgaacc
     1081 atcgcggtca cagcgagaag gcacagtcca gttccgataa ggatgcggat cagttgctcg
     1141 cgcaggcgcg gcacgatgat catcaccccg gtcgcgatcg ccagaggtgg cagaaggatg
     1201 gcgacgctcc cgacgaacag agtggcgaga taggcgaaga agcgtccgat cggtcctgca
     1261 gcccccgagt acacgccgag tccaccgatc gcacctagga atgaaacgaa cacgccccaa
     1321 ccctcgaccg gcacggcgcg gatccgttcg atggtccacc gggtcgcgac cagggtccgg
     1381 gggggacctc tgcgagcggg tcgtcgcgga ggggggcgct tcgctgcccg ccgtggggcg
     1441 ggtcgacgtg ccggagcacg ccgggacgaa gacctcggcg cgcggcgcct ggtggcggtg
     1501 gccatgcgtc ctcctagggg ggatgggtta acaactctaa caatagttaa caggaataac
     1561 agcatccccg cgggctgctc cgcaagcact tcggggcgtt aaataggggc atgcgagcta
     1621 tagctccggc ctgtgtcgtc ctggccctcg ccctggtggc ctgcgactca cccggctctg
     1681 ctccccctgc cgggctgccg tcggctcgag agacctcctc cgatccctca cccgaggagc
     1741 gcaccacgcc ccccaagagc gttccggagt cgaagcgtgc cccgatcgaa gttacgagcc
     1801 cggctcaggg cgatgaggta acgagcccgc tcatcgtcac cggaaccgcg aacgtcttcg
     1861 aagcgaccgt ttcgtaccag ctcgtagatg gctcgggcaa cgagatcaca aaggggttca
     1921 cgacggcctc ctgcgggacc ggctgtcgcg gcgactactc caagaagatc cgcttcaccg
     1981 tggacgaacc gaccgaagct acgctcgagg tgttcgaatc ctcggctgag gacggcaccg
     2041 cgctgcacgt ggtgcgcgtg agcctgacgc tgcttcctta atccagaacg atattgaagt
     2101 cgtagaaccc gtcgccggca tcccactgcg cgaacgctcg catgtagaag cgccccgagg
     2161 acggaagccg gaacttcgca tcccatactc gcccgcggcg gccgtcacgc ggctcgagcc
     2221 ggaactcgat gccgtgaggc cggccggccg gctctccgtt ctcgttgacc cgccgccaat
     2281 agtcgaggga catctgcttc ggtttctcgg ggtacttcag tacgacgcgg gccttggcgc
     2341 gggacttggc ttggtcgggc ggaggccagt cgaaggagaa ggtgtcctcg cacttcgatc
     2401 cactgggcga ttcccagcag tagctaccga ggttgccccg ctggcgcgtg tcgcccgaac
     2461 ggaacacgag gtccgggggc tgtgactcgt cctgggccgc caccggaacg accgatgcca
     2521 gagccacgaa cgtcagcaga cagataagag cgcgcttcac acttccatta tcacggggag
     2581 gatcatcggc cggcgacgcg tccgttgcca cacgaactcg ccgagggcct tacggcactc
     2641 cttcttgatg accccccaat cggtcacctg gtccctctcg agcttctcca gagcatcggc
     2701 tacacggtcg gccccctcgt ccagaagatc ccgctgttct tgttcgtgaa cgaatcccct
     2761 gctgatcagg tcgggcccgg cgaggatctc gccgttctgc gagtcgatcg tgaccacaca
     2821 catcacgatc ccgtcctcgg atagcactcg gcggtcacgc aacacaactg gtccgacgtc
     2881 gccgacaccc aggccgtcga ccagaaccat cccggcacga acgcgatcgc cgcgcttcac
     2941 ccgcccggag accagctgga ggacatcacc gtcttcgacc atccagatgt catcggaggc
     3001 cacgccctgc tgctccgcga tgcgcgcatg cttggccaga tgccggtatt caccgtgcac
     3061 cggacagaaa ttcttggggc cgaccgttct gatcatctcg gcaagctcat ccgaggccgc
     3121 gtgaccggaa acgtggacgc cgtcgtcctc gcctttgtag acctcggccc ccgccttgta
     3181 gagagcgttg acgacgcggt gaaccgcaga ctcgttgccg gggatgggac tggccgacag
     3241 gatcaccgtg tcgtctcttt cgacgctcac gagcttgtgt tcgcccgacg cgatcagggt
     3301 gagagcagag agcggctcac cttgcgaccc ggtgcatacg acgacggaac gagatgggtc
     3361 gacatcgttc atctcgccaa catccatcaa cgtcccgtcg gagatgctca gcaatcccag
     3421 ctcgcggccc acgtccacgt tgttgaccat cgagcgaccg acgaaggcca ctttcctgcc
     3481 ggtcgcctcg gccgcgtccg aaacctgctg gatgcggtgc aggttcgatg cgaagcacgc
     3541 gacgacgacc ctgcgttgcg cggccgcgaa gatctccttc agtcgcggcc cgaccaggga
     3601 ttcagagggc gtgcgccctt cctcttcggc gttcgtggag tcggacagca agagatcgac
     3661 tccctcccgg ccggcggcag cgaacgcctc gaggtccgtg ctccgcccgt cgatcggtga
     3721 atggtccagc ttgaaatccc ccgtgtagag gatcgtcccg cccggagtgc gtgtcagcac
     3781 cgcaagggcg tcgggtatcg agtgagagac gggcaggaac cggcatgcga aaggccccag
     3841 cttcagctcg ccgggagcag agatcggatt cagctcggcc ttcgtgtgga actcgtccag
     3901 cctcttgcgg gcaagcccca gtgtcagcgg cgttccgtaa accggcatgt cgatatcccg
     3961 catgagccag ttcaacgcgc ccacgtgatc ctcatggccg tgggtcagaa caacggcgag
     4021 gcagtccgcg gctcgatcgc gcagatacga gaagtccggc aggaccaagt cgacgcccag
     4081 catctcttcg ttcgggaacg aaagaccggc gtcgatcacg aggatcttgc cctcggattc
     4141 gagggcgatc atgttgcggc ctatctcgcc gaggcctccc aggaatatga cccgggttgg
     4201 tgcagcggtc acaccagacc ggagctttcc agcgcggccc tgatgacctt cttctcggca
     4261 tcggtagctt cgacgagagg aagtcgcgga ggtccgacct cgaccccgat catgttcagt
     4321 gccgccttga ccgggatcgg actagcgcct cccatgatgg tttggaacaa cggctggagc
     4381 tccaggaaga tccggcgcgc ttcgtcgacc ttcccgtcgg agtacgccga gaagatctgt
     4441 ttgatctgag ggccgaccag gtgtgagcac accgagatga tcgaggtgac gcctgcaccg
     4501 atgtgaggta gcaccaacgc gtcgtcaccg gagatcaact cgaagtcctg taacccctcg
     4561 gcatccagct cggatcgcaa cctggccgtt tcgcccgcgt cgccgaccgc atccttgtcg
     4621 cccacgatcc tctcgtggcg ggccagctcg atcatcgtcg aacgctcgat acgcctcccg
     4681 gtacggcccg ggatgtcgta cacgatcacg ggtgcggtgc tggcatccgc gatcgcgcgg
     4741 aagtgcgcca gcagaccgct ctgcggaggc ttcgagtagt acggggtcac caacagacag
     4801 gcatccacgc ccaactcaca cgcctctttc gtgagggtga tggactcggc ggtgttgttg
     4861 gtaccggtgc cggcgatcac tttcgcccgg tccccggccg actgcgccac cgtctcgaag
     4921 agagtgagct tctcgtcatg ggagagagtg ggcgattccc ccgtggttcc gcacacgatc
     4981 aggccctcgg agccgtgttc gatcaggtga tcaaccaaag ggggtacggc ttcgacatcg
     5041 agcgcgaagt ccttcttgaa cggcgtgacc attgcagtca tgacctcgcc gaatcgtgcg
     5101 ctcatagttg cctccccgat gtcgatcgcc ttgccgaaac gctacaggaa cgtcgagagc
     5161 ctcgttccct tagctcgatc aggctccgcg ccgtcgtaga cgaggatcgc gttcccgacc
     5221 tctccctcct cgttctcggg taacaccagc tcgaagcgga tctcgagagc ctctccttca
     5281 ggcaggccgc cgaagcgata gaccacggcg tcctgggaca cggccaccgg ctcaggcctc
     5341 acactcttca ccccatccgt tctgccaccc gtgcccacat cgactatcgg tgccggcaat
     5401 cccgcattcc cgatgcgcga gaacgcgacg acgaccgatt ccatgtcgcc cgggccgggg
     5461 ttcgcgatcc gtaccgcagc ggtcacggtc gacccgggtt ccgcctcgtt cggcatcagc
     5521 gcagtgactt gaggtttgcc ctttccggac tcggtcaggt tcggatccgg ttcgccgaca
     5581 caggctgcgg ccacgagcgc gagtagcgcc agagcaacgg tgcgtctcat agatccaaca
     5641 ggtgctcgag cccgactacg cacccatcca gcgaagccac cttcttgacc gccaggacca
     5701 cacccggcat gaaggaagcg cgatcgatcg aatcgtgccg gatcgtcagc gtctcacctg
     5761 cattgcccag gatcacctct tggtgggcga ccgagcccgg gacccgtacg gagtggatcc
     5821 ttagacccga ctcgtctccg cctctgaact cttccccgcg gcccctggtc acctcgtccc
     5881 acgcgcgtcc acgggcttcg ttcatcagat gggccgttcg ctgggcggta cccgaaggtg
     5941 cgtcgagctt cttctcctgg tgccgctcga tgatctcggc gttggagaag aaggctgcgg
     6001 cttcggccgc aaacttcatc atgaggacgg cacccagtgc gaagttcggt gcgatgaaag
     6061 cattagcggg tccggcttcg gcccacttcc gaacctcgtc gaagtcggac tcctcgaatc
     6121 ctgtggttcc gactacgacg tggatctcgt tctccagaca gaacctgatg ttgtccttaa
     6181 cgacgtccgg cgttgtgaag tcgacggcga cctcggcccc gttggcaagc agatcttcga
     6241 gcggctcatc ctgatcgatc ctggcaacga gctcgagatc ggtttcggcc tcgacggcgg
     6301 cgcacacggt cgcacccatc cgccccttcg cccccaagac tccgaccttc atggcgtcag
     6361 gcggcgtatt ggtcgaacgc gttctcgtcg aacggcccga cgaccgacac atggaagctg
     6421 cccgttccca gcacgttcgc tgcaacgcgc ctcagatcgt ccatctcgag cgcctcgaac
     6481 ttctcgatca gctcgtcgat cgagaggatc tcgccggtcg ttatctgttg gcggcccagg
     6541 cggttcatgc ggcttccggg atcctcggaa gaaagaacga gcgcaccctt cacatgaccc
     6601 ttcgcgcgcg ccagctcggc gtccgtcacc ccttcatcaa cgaccttctg gatctcgtcg
     6661 tggacgatcg agatcacgtc gtcggcattc tgcggggtcg tccccgcgta gaccgcgaac
     6721 gaaccggtat cggcgaacaa cgagcgatac gagtagacgg agtaagcgag cccgcgcttc
     6781 tcgcgcacct cttggaacag ccgcgacgac atcccgccgc cgaggatcgt gtccaggacg
     6841 gcgagggcat gccggtcggg gtgattccgt gggagcgctt cggttcccac gacgatgtga
     6901 gcctgctcgg tcggccggcg gtagaccttc acgccggcgc ggggcttcgg tgattcgatc
     6961 tcacgacccg tccgcgcgcc atcgcgatcc ccgagtagcc ccgcaacctt ctcgaccagc
     7021 acgtcgtgct taacgctccc ggcagcggca aggacgaggt tcaggggtcc gtacctgttc
     7081 tgccagtagg aggcaacctc gtccctcgac acggcaccga tcgtttcgtt gaagcccagc
     7141 acgggccgcc cgagagggtg cccatcccat agatatctgt agaacagatc gtggacgagt
     7201 tcgtcgggcg catcctcgtg catcgcgatc tcttccaaga tcactttccg ctccgactcg
     7261 aattccttcg gatcgatcaa cgatgccgag atcatgtccg acagcacgtc gaacgccatg
     7321 gggaggtcgg tgtcgagaac gcgggagtaa tagcacgtgt actccttgcc ggtgaaggcg
     7381 ttgacgtccc caccgaccgc atcgaacgca tcggcgatgt cctgggccga cctcctcggc
     7441 gtccccttga agagtaggtg ctcgaggaaa tgagacgtgc cggccagctc ggccggctcg
     7501 tcccgcgaac cgatgttgaa ccagaagcca agagacaccg agcgaacctc ggtcatctct
     7561 tcggtgacaa cggttgctcc cgaatcgaga accgtgcgag cgaaacgcgt catcgcttta
     7621 actgtcgtcg cggcgagggg ctctctctct acgcggtccc ccacctccgc cgccttcacg
     7681 gcgcgggcca ccgcggtcgc ggcgtggtcc tcctcggtcg cgatcacggc cgccgccgcc
     7741 ccggctgccg ccgctggagc tcttggccgc ctcgcgcgcc tcttcgatcg acatgccctc
     7801 ggccactttc cgctcgagcc atgcaaccgg ggtcaacgag atacgacctt ggcgatcgag
     7861 gtccgtgact tcgacctcca gctggtcccc cgcgttgacc gcatcctcga cccgctcgat
     7921 cctgccttca ccgagcttgc tgatgtgcac gaggccgtca cgtcccggtg agatgttcac
     7981 gaatgcaccg aaagtcgtcg tcttgaccac ttgtccgttg atccgctcgc ccatctcggg
     8041 catccgggga ttcgccgtct cttcgatcat cttggcggcc gcctcggccg attcctgatc
     8101 cgtcgagccg atgcgcacgg tgccgtcctc gtcgatgtcg atctcgacgc cggtctgggc
     8161 cgtgatggcg ttgatgttct tgcccttggg accgatgatc tcgccgatct tgtcgaccgg
     8221 gatcttcacc gcaagcaccc gcggagcgaa cgtcgccagc tcctcgtgag gatggtcgat
     8281 cgtcttgttc atctcgccga tgatgtgcag acgagccttc ttggcggcca tcagcgcgtc
     8341 acgcagaacc tcggcgggaa cgcccgagat cttcgtgtcc agctgcagcg cggtgactac
     8401 gtcctccgtg ccggcgacct tgaagtccat gtcgccgaac gcgtcctcgt ctccgaggat
     8461 gtcggtcaac ggctggaact tgccgtcctc cgcgatgaga cccatcgcca ctccgccgac
     8521 atgcttgccg ccgcggatcg gaacgcctgc gtccatcatt gccagcgacg aaccac
//
LOCUS       DATMED010000064         6307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4657115, whole genome shotgun sequence.
ACCESSION   DATMED010000064 DATMED010000000
VERSION     DATMED010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4657115"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2968
                     /locus_tag="VNP73_01685"
     CDS             <1..2968
                     /locus_tag="VNP73_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWL64658.1"
                     /translation="VAFVDYYSSDRILGVDEDGDPIDTNDAADVFVTVPGSGVYERVS
                     VASDGNQGGAIEADIRDPDPFASAQPRLSNDGRYVAFISRLQGLDPRVNSLERHAYVH
                     DRQTKTTEVVSINSRGDPVSANEVALSDDGRTVAFVSYERDFGARDRNPGDDVFVRDL
                     DTDKTVLASFDSTGRQFHNWRDRNSSTNHALAPYLSGDGRYLTFIGPRDTDPTVPAPL
                     VDETVWVRDLDRNRTIKGSLGSHGHLKGQSLNPSLTPDGLLLSYESSVRFLQEDTNGL
                     TDVFVRKLRPSADLSGTVVDGKLFPDGHAEPVAGAMVELRDEGRTVYGPTATDGEGGY
                     SFRNIVPGSYRLRVTFEDARYMPSSGMPPSHLEPIFDVRYTENATGPLWVEVDVEVED
                     ADRRKDVAVSRSETLVAVSADVPRAEWGHLDDMAAIFTSTQKYIGWLIKEISPVMESD
                     RVPLPVPIHVYSRARNLAAGPFNAFHLYEENAPIKSVIHIGTGLSDYERRDGVGGGDW
                     GPFTEWHEFTHHLSWVNGIGTHPDDRCVAVNHKGYNNPSTCDSVLEGVAAFLPTVAYP
                     ELMPSSGSPAGVYPLFNENLESNGLKAFSGNARDGEFSSWEEDAVAALLWDLYDPNAD
                     QHALSLVGADGVATSAAVTDSMNLPLRTIWDLLATRKTVRFLWQNTYIDRKLDEPIRL
                     SLDLDGDGTLDTSPFDVPFLLHGFYPLGDGDFATARRHYALQDASRFTGNSPNRAIGR
                     TDSPNPRTSDYRQRQVTPTEDRSTIAVEVLERSGTPVEDGFVVLRVSYPEGFTDEIRI
                     ALTGEGAEEIALRLPPHSDVPLDSATAGSLSCEDTEVDYPVEVSMFAVADGVESEKRV
                     SFSNCEYGVATVMADTGEPALTESFKLPRRRAKLAVRARTTRAGVLVRGKLTPGQPGS
                     PVVITLQRKRDGWQTVDRHGARLGPSIDRNGDRIGESVFRAKLGRVEGGRCRVLVTWA
                     GDTSHFGTKEAVGFSC"
     gene            2993..4213
                     /locus_tag="VNP73_01690"
     CDS             2993..4213
                     /locus_tag="VNP73_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL64659.1"
                     /translation="METSRETEPGRRRALVLLATTLVLSMSTWFSASAVIPQLRTEWG
                     LTDSSAAWLTIAVQLGFVTGAVFSAVLSLSDIFHPKHVILGGSIGAALANALVALAGG
                     AAWGIPLRFATGFFLAGVYPSAFKLMSTWTRKSRGVSLGILAGALVTGNGMPHLVNGL
                     GGLDWRVVIYGTTILTIAGGLLAELAVDEGPYPFPKATFDPRQAGRVFGNRGVRLASF
                     GYFGHMWELFAAYAWFLVFFSAHLELRGEGTSTTAAYTTFGFFLVSGFGCWAGGVLGD
                     RWGRTKTTALMMAVSGSCCLLIGTLFGGPTWLVLLVALVWGFAIVADSAQFSTMVTEL
                     ASQAYVGTALTLQLAVGFLITVATIWLIPMFEAAFGWQWAFALLAPGPALGVLAMLRL
                     KRAPDAARIAGGRG"
     gene            4234..4755
                     /locus_tag="VNP73_01695"
     CDS             4234..4755
                     /locus_tag="VNP73_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64660.1"
                     /translation="MTGRIALVAMLIVVGLAAPVGAVRNGDPEDMDGKLDIGLTRFVE
                     PEEGIAQATIVTYESWGCAHLGKGRGTTLRWLFDGRGDRRFDLVGTFECRRKQLLFDL
                     RSRNGSNMYESIEVRRRGDSRTALVRFPTDLPELERGDITMIAVSNDVSGETCQNVCR
                     DRAPDKGRLRFND"
     gene            complement(4760..5398)
                     /locus_tag="VNP73_01700"
     CDS             complement(4760..5398)
                     /locus_tag="VNP73_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL64661.1"
                     /translation="MNAIRLFVVDDHLLFLSGVKSELGDAFTIVGDANDVDDAISQIS
                     VAEPDVVLIDVHMPGGGGRRVIEEVSKSRPDVRFLALSVSDAAEDVIGVVRAGARGYV
                     TKTIAPVELEEAIRRVHEGDAVFSPRLAGFVLDAFNAEGIELTDPELDQLSQREREVM
                     RHIARGFTYKETAGRLHISIKTVETHVSSVLRKLQLSNRHEITRWATDRRLV"
     gene            complement(5395..>6307)
                     /locus_tag="VNP73_01705"
     CDS             complement(5395..>6307)
                     /locus_tag="VNP73_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL64662.1"
                     /translation="WFGDAVVWPSAIAILGALTIWLRSDPSESSRVRRVAASVTVSPL
                     EAIVAGRSSRSGVLIGAALTVAGTAAFLAANTSLESLGNLIFPVAVTIAGLGLILGPW
                     VARLTRQASDERQERVRSQERSEMAAHLHDSVLQTLALIQRTESPRRMVSLARTQERE
                     LRAWLYDRTGDLDEESMRTAFDALAGKIEQTHLVRVETVVVGDAPMDDRTRALVKACG
                     EAINNAARHAGSQEISVYVEANAQEVIAYVRDEGVGFDVDAIPEDRHGIRESICARME
                     RIGGAATISSTTGEGTEVQLRMARMAP"
BASE COUNT         1109 a         1876 c         2070 g         1252 t
ORIGIN      
        1 cgtcgcgttc gtcgattact acagcagcga tcgcatcttg ggagtcgatg aggacgggga
       61 tccgatcgat accaacgacg cggccgatgt gttcgtgacc gtgccgggct cgggtgtgta
      121 cgagcgcgtt tcggttgcga gcgacggaaa ccaaggcgga gcgatcgagg cggacatccg
      181 cgatccggac ccgttcgcct cggcccagcc ccgtttgagt aacgacggac gctacgtcgc
      241 gttcatctcg cgcctgcagg gtctggaccc gcgcgtgaat tcactcgagc gacacgctta
      301 tgtgcacgat cgccagacga agacgaccga agtcgtgagc atcaactcgc gaggcgatcc
      361 ggtttcggcg aacgaggtcg ctctgagtga cgatggacgc acggtcgcct tcgtctccta
      421 cgagcgcgac ttcggcgcga gggatcgcaa tccgggcgac gacgtattcg ttagagatct
      481 cgatacggac aagaccgttc tcgccagttt cgattccacg ggtaggcagt tccacaactg
      541 gcgcgatcgc aacagcagca cgaatcacgc gctcgcgccg tacctcagcg gtgacggccg
      601 gtatttgacg ttcatcggtc ctcgtgacac cgacccgacg gtgcccgccc cgttggtgga
      661 cgagacggtt tgggtgcgcg acctggatcg gaaccgcacg atcaagggga gcctcgggtc
      721 acatggccat ctgaagggtc agagcttgaa tccatccctt acgcccgatg gcttgcttct
      781 ctcgtacgag tcgagcgtga gattcctgca ggaggacacg aacggattga cggacgtgtt
      841 cgtacggaaa ctcagaccgt ccgcagatct gtcggggacc gtcgtggacg ggaagctgtt
      901 cccggacgga catgcggagc ccgtggcggg ggccatggtc gagttgcggg acgaaggccg
      961 gacggtctat gggccgacgg ccaccgacgg ggaagggggc tacagcttcc gcaacatcgt
     1021 tccgggttcc taccggttgc gagtgacgtt cgaagatgcc cgctacatgc cctcgagtgg
     1081 gatgccccct agtcatctcg aaccgatctt tgatgtgcgt tacacggaga acgcgaccgg
     1141 accgttgtgg gtcgaagtcg acgtcgaggt ggaagacgcg gaccggagaa aggacgtggc
     1201 cgtcagtcgc tcggaaactc tggtggcggt ttccgccgat gtccccagag ccgaatgggg
     1261 gcacctggac gacatggcgg cgatattcac atcgacacag aagtacatcg gttggctgat
     1321 caaagagatc tcgccggtga tggaaagtga cagggttccg ctcccggtcc cgattcacgt
     1381 ttattcgcgg gcccgaaatc tggcggccgg accgttcaat gcgtttcacc tctacgagga
     1441 gaacgcacct atcaagagcg tgattcacat cggcacggga ttgtcggact acgagcgccg
     1501 cgacggcgtg ggtggagggg actgggggcc gttcaccgaa tggcatgagt tcactcatca
     1561 cctttcgtgg gtaaacggga tcggaacgca tcccgacgat cgttgtgtgg ccgtgaatca
     1621 caagggctat aacaatccca gcacctgcga ttcggtcctc gaaggtgtgg cggcgttcct
     1681 tccgacggtc gcgtatcccg aactgatgcc ctcttccggt agccctgcgg gcgtgtatcc
     1741 gctgttcaac gagaatctgg agtcgaacgg tctcaaggct ttcagcggca acgccaggga
     1801 cggcgagttt tcgtcgtggg aggaggacgc tgtcgcggca ctgctgtggg atctctacga
     1861 ccccaacgcc gaccaacacg cactgtcact cgtaggggcc gacggcgtcg ctacgtctgc
     1921 tgcggtaacc gattcgatga accttccgct gagaaccatc tgggatctgt tggcgacgcg
     1981 aaagacggtc aggttcctgt ggcagaacac gtacatcgac cgcaagttag acgaacctat
     2041 ccgtctttct ttggacctgg atggtgacgg gacactcgat acgtcgccct tcgacgtccc
     2101 cttcctgttg cacggcttct atccgctcgg agatggagac ttcgcgacgg cgcggcgcca
     2161 ctacgccctg caggatgctt cgcggttcac tggtaattcg ccaaaccggg cgatcggccg
     2221 gacggattca cccaatccga ggacgagtga ttatcgccaa cgtcaggtga caccgaccga
     2281 ggatcgatcc acgatcgcgg tggaggtgct cgagcgaagt ggcacgccgg tcgaggacgg
     2341 attcgtcgtt ctacgggttt cttatcctga gggtttcacc gacgagattc gtattgccct
     2401 tacgggtgag ggggccgagg agatcgcgct gcggttgcca cctcattcag atgtaccgct
     2461 cgactcggca accgcggggt ccctaagttg cgaggacacc gaggtcgatt acccggtcga
     2521 ggtctcgatg ttcgccgtgg cggacggggt ggagagcgag aagagagttt cgttttcaaa
     2581 ttgcgagtac ggcgttgcta cggtgatggc cgacactggc gaaccggcgc taacagagtc
     2641 tttcaagttg ccgaggaggc gggcgaagct ggccgttcga gctcgcacga ctagagcggg
     2701 tgttctggtc agaggaaagt tgaccccggg ccagcccggt tccccggtgg tgatcacgtt
     2761 gcaacgaaag cgtgacggtt ggcagacggt cgatcgtcat ggggcgcggt tgggtccgtc
     2821 gatcgatcgc aacggtgacc ggatcggcga gagcgtgttc cgggcgaagc tcggccgggt
     2881 cgaaggtggc cgctgccgcg tgctcgtgac ctgggccggt gacaccagtc atttcggtac
     2941 caaggaggcg gtcgggttca gctgctgacc ggtcccgtct agattgcctc ggttggaaac
     3001 cagtagagaa accgagccgg gtcggaggcg cgccctcgtt ctcctcgcga ccactctcgt
     3061 gctgtcgatg tcgacgtggt tctcggcgtc ggccgtcatc cctcaactga gaaccgagtg
     3121 gggtctcacg gatagttccg ctgcgtggct cacgatcgcg gtgcagctcg gcttcgtaac
     3181 gggagcagtc ttctcggccg tgcttagcct ctccgacatc ttccatccga aacacgtcat
     3241 cctcggcgga tcgatcggag cggcccttgc caacgcactt gtcgcgctcg ccggaggtgc
     3301 ggcgtggggg atcccgttgc gtttcgcaac cggtttcttc ttggccggtg tctacccatc
     3361 ggcgttcaag ttgatgtcga cgtggacccg caagagtcgc ggcgtgtcgc tcggcatcct
     3421 cgccggtgcg ctggtgacgg gaaacggcat gccccatctc gtcaacggct tgggcgggct
     3481 cgactggcga gtcgtgatct atgggacgac catccttacg atcgccggtg ggctgctcgc
     3541 ggagttggcg gtggacgagg gcccgtaccc gtttcccaag gcgaccttcg atccgcgcca
     3601 ggcgggccgc gtcttcggca atcggggcgt ccggctcgcc tcgttcggtt acttcggtca
     3661 catgtgggag ttgttcgccg cgtacgcatg gttcctggtg ttcttctcag cccatctcga
     3721 gttgcggggc gaggggactt caaccacggc tgcctacacg acgttcgggt tcttcctggt
     3781 gagcggtttc ggctgttggg ccggaggggt cttaggcgat cgctggggcc gcacgaagac
     3841 gaccgcgttg atgatggccg tctccggtag ttgttgcctc ctgatcggaa cgttgttcgg
     3901 tggtcctacg tggctggtgt tgctggtcgc gctcgtgtgg ggattcgcga tcgtcgccga
     3961 ctcggcgcag ttctcgacga tggtcaccga gcttgcgtcg caggcctacg ttggcacggc
     4021 ccttacgttg cagcttgcgg tcggattcct gatcaccgtt gcgaccatct ggctgatccc
     4081 gatgttcgaa gccgcgttcg ggtggcagtg ggcttttgcc ctgctggcgc cgggacccgc
     4141 actcggcgtt cttgcgatgc tgcgactgaa acgagcgccg gatgcggctc gtatagccgg
     4201 cggtcggggg taagggttct agacttcagc ctgatgactg gacggatcgc cctcgtcgcg
     4261 atgctcatag ttgtcgggct tgccgctcct gtgggtgcgg tgcgcaatgg ggaccccgaa
     4321 gacatggacg ggaagctcga catcggactc acgcgtttcg tcgagcccga agagggcatc
     4381 gcccaagcga cgatcgttac gtacgagtcg tggggctgtg ctcatctcgg caagggccgg
     4441 ggaacaaccc tcaggtggct cttcgacgga cgtggggacc gtcggttcga tctcgtcggt
     4501 acgttcgagt gcaggcggaa gcagttgctg ttcgatctcc gttcgaggaa cgggtcgaac
     4561 atgtacgagt cgatcgaggt ccgtcggcga ggtgacagcc gaacggcact ggtcaggttc
     4621 ccgacggatc ttcccgagtt ggaacgcggc gacatcacga tgattgctgt cagcaacgat
     4681 gtgagcggtg agacctgcca gaacgtatgc cgtgaccggg ccccggataa aggccgcctg
     4741 cgcttcaacg actagtggct cacaccaacc tgcggtccgt cgcccagcgg gtgatctcgt
     4801 gtcgattcga gagttgaagt ttcctaagca ccgacgacac gtgcgtctcg acggtcttga
     4861 tcgagatgtg tagccgaccc gccgtctcct tgtaggtgaa gcctcgggcg atgtgacgca
     4921 tgacttcgcg ctcgcgctgg ctaagctgat ccaattcggg atcggtcagc tcgatgccct
     4981 cggcgttgaa tgcgtcgagc acgaagccgg caagacgtgg cgagaagacg gcgtcaccct
     5041 cgtgtacgcg gcggatcgct tcctctaact ccaccggtgc gatcgttttg gtcacgtagc
     5101 cgcgggctcc ggcgcgcacc accccgatca cgtcctcggc cgcatccgac acagaaagtg
     5161 cgaggaaccg cacatccggc cggctcttcg agacctcttc gatcacacgg cgaccaccgc
     5221 ctccaggcat gtggacgtcg atcagaacga cgtcgggttc ggccaccgag atctgcgaga
     5281 tcgcatcgtc gacgtcgttc gcgtccccga cgatggtgaa cgcatctccg agttcggatt
     5341 tgacgccgga aaggaacagc aggtgatcgt caaccacgaa aagtcggatc gcgttcatgg
     5401 agccatcctg gccatccgga gctgaacttc ggttccttcg ccggtcgtgc tcgagatggt
     5461 cgcggcgcct ccgatgcgct ccatccgggc gcagatcgac tcccggatcc cgtggcggtc
     5521 ctcggggatc gcatcgacgt cgaagccaac accttcatcg cgcacatacg cgatcacttc
     5581 ctgtgcgttc gcctcgacgt acacggagat ctcttgggag ccggcgtgcc tggcggcgtt
     5641 attgatggct tcgccgcagg ccttcacaag ggcgcgggtc cgatcatcca tcggtgcgtc
     5701 acccacgacc acggtttcaa ctcgaacgag atgagtttgc tcgatcttgc ccgccagcgc
     5761 gtcaaaggca gtgcgcatgg attcctcatc gagatcgccc gtgcggtcgt aaagccacgc
     5821 tctcagttcg cgttcttgtg ttcgcgcaag gctcaccatc cgacgcggtg attctgttcg
     5881 ctggatcagg gcgagtgtct gaagtactga atcgtgcaag tgggccgcca tctccgaacg
     5941 ctcctgcgac cggactcgct cctgccgctc gtcggaagcc tgtcgagtga ggcgcgcgac
     6001 ccaaggaccg aggatcaagc cgagtccggc gatggtcacg gcaacgggaa agatgaggtt
     6061 gccaaggctc tccagcgagg tgttcgcggc aaggaaggcc gccgtcccgg cgacggttag
     6121 ggcggctccg atcaatacgc ccgagcggga actgcgtccg gcaacgatcg cctcgagcgg
     6181 actcaccgtc acacttgcag cgactctccg cacgcgcgag ctctcggagg gatcgctccg
     6241 gagccagatc gtgagtgcgc cgagaatcgc gatggccgac ggccacacga cggcatcgcc
     6301 gaaccac
//
LOCUS       DATMED010000065         3766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_51541, whole genome shotgun sequence.
ACCESSION   DATMED010000065 DATMED010000000
VERSION     DATMED010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51541"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VNP73_01710"
     CDS             <1..552
                     /locus_tag="VNP73_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HWL64663.1"
                     /translation="VQVPNERDDASTIKIELELPQVFAFASFQDVSGWNRTEETVEFD
                     EPIEAFGEEVTEGIGSVTWEGAVIGPGEFERFAISVGPVPTGPIEFKAIQTYDSGEVV
                     RWIGPADSDEPAPVVNGIDLGVEEGQGQLAALADIKAQLDALAAPEEEDSTYDSGSLL
                     LPGAALVVSLIALLVAFTRKRTT"
     gene            553..1050
                     /locus_tag="VNP73_01715"
     CDS             553..1050
                     /locus_tag="VNP73_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64664.1"
                     /translation="MVLAHTIGGPSGLEIELLLVALGFLAAAFFFRPSQAGGSARTMV
                     FCLVIGVGLLAGSVAFPRLSGSETQAQVAITAPQDGAAVPANEPVVLTVDLTGGSLVS
                     GPTDTTGGHLHVFVDNKVQSMPSSLRIRVPFEPGSHEVRIEYVDTQHLSFEPPVTDTI
                     TIQAN"
     gene            1052..2293
                     /locus_tag="VNP73_01720"
     CDS             1052..2293
                     /locus_tag="VNP73_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64665.1"
                     /translation="MVLTHGVGSVYESPLPLSLYLVGAAGTVLISFVLRALVTADPRA
                     SGQRTIMGPATADVLIALGRAVALIGLLLTVIFAVVDPERGFSVAPLLFWVGLVISTA
                     LLEAICAGIWTQTNAWTTLKDLLRLPDRERQLPWWLGPLLVYVLFWFELVSGRGFDPL
                     VIMVVLLAYTLFVFAVGDDREEVDPLSILFGFAGRCAPLQLTDAGVVYKGPLSDLAEE
                     RPMPRGLMGAVFLLLAATTLDNMRETVEWNSLLNNLGLDSAPHVVVDSIALLLFTLPF
                     LLPFLAAVWIAGRWLNGDSLSARLAWSLIPIGVAYLIGHNLPLLITGLPQLAVQIGEQ
                     FGLQLFGNYIPSPKLVWFLEIAVIVGGHVLGVLSSHRIAARLADSHGAALKSHTTLTV
                     LMCAFTVATLWLLSLPLVVQA"
     gene            2438..2575
                     /locus_tag="VNP73_01725"
     CDS             2438..2575
                     /locus_tag="VNP73_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HWL64666.1"
                     /translation="MRDNERGATSVEYALIASLIAAVIAAVVTLVGEEVLQMFESVTW
                     W"
     gene            complement(2583..>3766)
                     /locus_tag="VNP73_01730"
     CDS             complement(2583..>3766)
                     /locus_tag="VNP73_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL64667.1"
                     /translation="SSTGVTLDLGAGHASGPGTDTLSSIENVNGSRLDDSLTGSDRAN
                     YLVGELGNDTINAGAGEDVIQPGSYGELTPDDDTIDGGSEVDLFQFWDAGHAITIDFS
                     EGIATGEGTDHFTGMEGMTDSPHDDTIHGSDGDEIFIAYEGNDTIDGRGGFDILAYWL
                     SDNPVTVDLEAGTASGYGRDSIAGIEAAWGSNDRRNEMYGDAGNNILWGGEVDDKIYG
                     RGGDDYIGGFIGDDLFDGGDGAFDIGTFCAPCTPAIRADMRTNKVTGPGDVTLVGIEA
                     IEGSPSDDVIFGNDATNWIYGLTGDDKINGLGGDDFLAGDQGEDKADGGDGDDNCYST
                     ENPLSCESVHGGGESLTCIPVLAVRACQNEGDPDAPPLHPLQLTETATEALQVKFKLN
                     F"
BASE COUNT          616 a         1188 c         1164 g          798 t
ORIGIN      
        1 gtgcaggtac cgaacgagag ggacgacgcc tcaacgatca agattgagct ggagcttccg
       61 caggtgttcg ctttcgcatc attccaggac gtaagcgggt ggaacaggac cgaggagacg
      121 gtcgagttcg acgagccgat cgaggcgttc ggcgaggagg ttaccgaagg gatcggatcg
      181 gtcacgtggg aaggggcggt gataggcccg ggtgagttcg agcgcttcgc gatctccgtc
      241 ggtccggtcc cgaccggtcc catcgagttc aaggcgatcc agacctatga ctccggagag
      301 gtcgtacggt ggatcggccc tgcggattcg gatgagccgg cgccggtagt gaacgggatc
      361 gacctcggcg tagaagaagg gcagggccaa ctggccgccc tcgccgatat caaggctcag
      421 ctggacgcgc tggccgcacc ggaggaagag gattcgacct acgattcggg ttcactgctg
      481 cttccgggcg cggccctcgt cgtgtccttg atagctctgt tggttgcgtt cacgagaaag
      541 cgaacaacgt aggtggtcct tgcccacacg ataggggggc cgagcggcct cgagatcgaa
      601 ctgcttctcg tcgctcttgg cttcctcgcc gcggcgttct tcttccggcc ttcacaggcc
      661 ggagggagcg cgcgcacgat ggttttctgc ctcgtgatcg gtgtcgggct tctcgccggg
      721 tcggtcgcat ttccacgatt gagcggatcc gagacccagg cgcaggtggc catcaccgct
      781 ccccaggacg gtgccgcggt gccggcgaac gagccggttg tgctgacggt agatctgacc
      841 gggggctctc tggtgtcggg tcccacagac acgacggggg gtcaccttca cgtgttcgtg
      901 gacaacaagg tgcaatcgat gcccagctcg ctacggatcc gcgtcccctt cgagccgggc
      961 tcacacgagg tccggatcga gtatgtcgac actcaacacc tctcgttcga gcctcccgtc
     1021 accgacacga tcacgatcca agccaactga tttggtcctg acccacggcg tcggatccgt
     1081 ttacgaatcg cccctgccgc tatcgctcta cctagtagga gcggccggga cggtcctcat
     1141 atcgttcgtg ctgcgcgcgc tggtaactgc cgatccaaga gcgtcagggc agagaaccat
     1201 catgggtccg gcgaccgccg acgtacttat cgcgttgggt cgagcagttg ccctcatcgg
     1261 actgttgctc acggtgattt tcgcagtggt cgaccccgaa cgggggttct cggttgctcc
     1321 actgcttttc tgggtagggc tcgtcatctc caccgctctc ctcgaggcga tctgcgccgg
     1381 gatctggaca caaaccaacg cgtggacaac gcttaaagat cttcttcgct tacccgaccg
     1441 cgagaggcaa ctaccgtggt ggctgggccc cctcctcgtg tacgtcctct tctggttcga
     1501 gctcgtgtcc ggccgcggat tcgaccccct tgtgatcatg gtggtgctac tcgcctacac
     1561 cttgttcgtg ttcgcggtgg gcgacgatcg ggaggaggtc gaccctctct cgatcctgtt
     1621 cggattcgcc gggcgttgcg ctcccttgca attgaccgat gccggcgtgg tctacaaggg
     1681 accgttatca gatctcgctg aggaacgtcc gatgccgagg ggcctcatgg gggcggtctt
     1741 cctattactc gcggcaacca ctctcgacaa catgagggaa accgtcgagt ggaactccct
     1801 gctcaacaat ctgggattgg actcggcccc gcatgttgtc gtcgattcga tagctctcct
     1861 cctcttcacg ctcccattcc tgttgccgtt cctggcggcc gtgtggatcg cggggcggtg
     1921 gctcaacgga gactcgttat cagcgcgctt agcctggtcg ttgatcccga tcggtgttgc
     1981 atacctgatc ggtcacaacc tccctctctt gatcacgggc ctgcctcaac tcgctgttca
     2041 gatcggggag cagttcggcc ttcagctgtt cgggaattac atcccctccc ccaagctggt
     2101 gtggttcctc gagatcgcag tcatcgttgg gggacacgtc ctcggtgtgc tgtcatctca
     2161 tcgcatagcc gcacggctgg ccgactcgca cggcgccgcc ttgaagagcc acacgacgct
     2221 caccgtcctc atgtgcgcct tcaccgtcgc gaccctctgg cttctctccc tgcccttggt
     2281 cgtccaggcc tgaaaccggg ttcaagtcgc gggcacttct ctccgataac tagtcaaacc
     2341 gaactagtgc tggtggtgca gtgcgcgatc gtctcgacga tcgcgccgtc cgaagcgaag
     2401 ggctgcatgc gcagcaggca tacctgaggg aggacaaatg agagacaacg agcgtggggc
     2461 cacgtccgtc gaatacgcct tgatcgcctc cctgatcgca gccgtgatcg ccgcagtggt
     2521 cacgctcgtc ggagaagagg tgcttcagat gttcgaaagc gttacgtggt ggtagcgaat
     2581 ccttagaagt tcagcttgaa cttcacctgc agggcttccg tggcggtttc cgtcagctgc
     2641 aatgggtgaa gcgggggagc atcgggatcg ccctcgttct ggcacgcgcg cactgcaagc
     2701 accgggatgc aggtgaggct ctcgccaccc ccgtggaccg actcgcacga cagaggattc
     2761 tcggtcgagt agcagttgtc gtccccgtca ccaccgtcgg ccttgtcctc cccttggtct
     2821 ccggcgagga agtcgtcacc gccgagcccg ttgatcttgt cgtcgccggt caacccgtag
     2881 atccagttgg tggcgtcgtt gccgaagatc acgtcgtcgc tgggggagcc ctcgatcgct
     2941 tcgataccga ccagggtgac gtcacccgga ccagtgacct tgttcgttct catgtcggcg
     3001 cggatcgcag gcgtgcacgg cgcgcaaaag gttccgatat cgaaagcgcc gtcaccaccg
     3061 tcgaacaggt cgtcccctat gaaacccccg atgtaatcgt ccccgccacg tccgtagatc
     3121 ttgtcgtcga cctctccacc ccacaggatg ttgttgcccg catccccgta catctcgttg
     3181 cggcgatcgt tgctgcccca cgctgcctcg atgccggcga tcgagtcccg gccgtagccc
     3241 gaggcggttc cggcctcgag gtcgaccgtc acggggttgt ctgaaagcca ataggcgagg
     3301 atgtcgaagc caccacgccc gtcgatcgtg tcgttgcctt cgtaggcgat gaagatctcg
     3361 tcgccgtcac tgccgtggat cgtgtcgtcg tgcggcgagt cggtcatgcc ttccattccg
     3421 gtgaagtggt cggtgccctc gccggttgcg atcccttccg agaagtcgat cgttattgca
     3481 tgtcctgcat cccagaactg gaagaggtcg acctccgagc ctccgtcgat cgtgtcgtcg
     3541 tccggggtca gctcgccgta cgaccccggc tggatgacgt cctcgccggc ccccgcgttg
     3601 atcgtgtcgt tcccgagttc gccgactaga tagttggccc ggtcgcttcc ggttagggaa
     3661 tcgtccaacc gggatccatt cacgttctcg atcgatgaca gggtgtcggt tcccggcccc
     3721 gaggcgtgac ccgcgccgag gtcgagcgtc acgccggtcg aggatt
//
LOCUS       DATMED010000066         3107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2356893, whole genome shotgun sequence.
ACCESSION   DATMED010000066 DATMED010000000
VERSION     DATMED010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356893"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VNP73_01735"
     CDS             complement(<1..1415)
                     /locus_tag="VNP73_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWL64668.1"
                     /translation="MTTTKQFDLDEAQRGVADHGTGPLLLLGGPGTGKTVALKERFIS
                     LAGQRSRGTGGCTSDRVLFLVPNRTQKMALQDELTRRLLFEEDLDALIEVPIYTWHGL
                     ANHLVTRHYDRLAYPEPPVLLTSPEQWGDVRDALGNEDEVNWPHHRHLLRNRGFVDEV
                     VDFCIRAEQRLLDDTQLEALAAARPAWGDIVRFFKSHRVRLRSRSRVDYPTLLADAAD
                     LIANHDDVREGLHQRFLHILVDDGQELALVQQRLLHFLAGFDSGAGAGRSLVLAGDPD
                     SAIETFRGADPDWIEDFAKEFGAHQSLILETSYRLGADHGHKTIGMIERNRAQSHRAQ
                     TFAGDSQLEVKRFPSLATEVEAIARDLRLAHLQDHVPYEDMAILLTSPRSMLPPLERA
                     LTSLEVPFSITAPDRPLGREPIVRAFVDLADFALEGEPDPEQLVTLLRSPLIDLEDAT
                     VRELERIARLSGKTLAETLEGR"
     gene            complement(1525..>3107)
                     /locus_tag="VNP73_01740"
     CDS             complement(1525..>3107)
                     /locus_tag="VNP73_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64669.1"
                     /translation="EAAAAWSVDFDVDQLKGLLPDRESSDVLSCLADLRSSGFIEDSS
                     TAAGSSTFRIRNALLREAAYLQCKKERLAELHERAAAYAEVQGYGPDFMGYHLERAFR
                     LRKEAVPDDPRNGSVAEKAANHLHAAGRGERARGATTSAADLFRRALDLRLVLGPAWF
                     DDSIELTDCLLDLARTREALEMVKLIQETAPQLGDAVVSARADLMKVFAKSLTSTDPT
                     IEKSFRELLPIFEAQGDHRTLVKIYRGLSEFYWNLFECGASASAAESALQHSLETPEK
                     FELAHIVSAVSYGLIWGPAPVSEAMAAGERLLTISVGNLVSLAQLKLKLGLLDAFAGN
                     FDAARKRAESGLRELRELGQERFAAIRTHDVGIVQMMAGELTTAYETFLAGYNELTRL
                     DMIDYRATSAAYLARIDYELGRAEQALKYLAIAEETAEEDPWIEPDTLPLRAQMAADA
                     GERDRALKFIGAARALADGKDSTVIQATIEILTARALRTLGMEEKAAEAQAKAEALCK
                     KKEAPALLALADPTSMKS"
BASE COUNT          579 a          974 c          977 g          577 t
ORIGIN      
        1 cgaccctcca gcgtctcggc cagtgtcttg cccgacaagc gagcgatgcg ttcgagttcg
       61 cgcacggtcg cgtcctcgag atcgatcaac ggtgaccgga gaagagtgac caattgttcg
      121 ggatcgggct caccttcaag cgcaaaatcg gctaggtcca cgaatgctct gacgatcggc
      181 tcgcgcccca gcggacgatc cggagccgtg atcgagaagg gaacctcgag cgacgtgagc
      241 gcacgctcca gagggggcag catcgaacga ggcgatgtga gcaggatcgc catgtcctcg
      301 taagggacgt ggtcttgcag atgagcaagt cgcaggtcgc gtgcgatcgc ctcgacctcg
      361 gtcgcgaggc tggggaaccg cttgacctcg agttgcgaat caccggcgaa cgtctgagcg
      421 cgatgggact gggcccgatt gcgctcgatc atcccgatcg tcttgtgacc gtggtcggca
      481 cccaagcggt acgacgtctc gaggatgagt gactgatgcg cgccgaactc cttcgcgaag
      541 tcctcgatcc agtcgggatc ggcgccgcgg aacgtctcga tcgccgaatc ggggtcgccg
      601 gccaacacga gcgagcggcc tgcacccgcg cccgaatcga acccggcaag gaaatgaagc
      661 agtcgctgtt gcacgagcgc cagctcttga ccgtcgtcga ccaggatgtg caggaagcgc
      721 tgatgcaggc cctcgcgcac gtcgtcgtgg ttcgcgatca gatcggccgc atcggcgagc
      781 aacgtcgggt agtccacgcg cgacctggac cgaagccgca cgcgatgcga tttgaagaaa
      841 cggacgatgt caccccacgc cggccgggcc gccgcgaggg cttccagctg cgtgtcgtcg
      901 agcaggcgtt gctcggcccg gatgcagaaa tcgacgacct cgtctacgaa gccgcggttc
      961 ctcagcaagt ggcggtgatg cggccagttg acctcgtcct cgttgccgag tgcgtcgcgc
     1021 acgtcgcccc attgctcggg cgaggtgagt aggaccggag gctcgggata agcgagccgg
     1081 tcgtaatgac gggtgacgag atggttcgcg agtccgtgcc acgtgtagat cggaacctcg
     1141 atcagggcgt cgagatcctc ttcgaacagg agacggcggg tcagttcgtc ctggagcgcc
     1201 atcttttggg tccggttcgg aaccaggaac agaacgcggt ccgaggtgca cccgccggtc
     1261 ccgcgggacc gctgacccgc gagggagatg aagcgctcct tcagggcgac ggtcttgccc
     1321 gtccccggcc cccccaatag aaggagtggg ccggtgccgt ggtcggcaac cccccgttgc
     1381 gcttcgtcga gatcgaactg cttggtcgtg gtcactgcct caccgtcgcc tcaccaatca
     1441 tccgaaggta ataggtgact gagacgttag tcagggggtg tgacagaaat cacgcattac
     1501 cgggttatcc acaggttttc ctgcttagga cttcatggag gtcgggtccg cgagggccaa
     1561 aagggcgggt gcctccttct tcttacaaag ggcctcggcc ttggcctgag cttcggcggc
     1621 cttctcttcc atcccaagag tccttagagc tcgcgccgtg aggatctcga tcgtggcctg
     1681 gatcaccgtc gagtccttac cgtcggcaag ggccctggcg gctccgatga acttcagcgc
     1741 ccggtcccgc tctccggcgt cggctgccat ctgtgcccgg agaggaaggg tgtccggctc
     1801 gatccaggga tcctcctcgg cggtctcttc agcgatggcg agatacttca gcgcctgctc
     1861 tgcccgaccg agctcgtagt cgatgcgggc aaggtatgcg gcgctcgtcg cgcggtaatc
     1921 gatcatgtcc aggcgggtca gctcgttgta tccggcgagg aacgtttcgt aggcggttgt
     1981 gagttccccc gccatcatct ggactatccc gacgtcgtgc gttcgtatcg ccgcgaacct
     2041 ttcctgtccc agttccctca gttcacggag accactctcg gcccgctttc gtgccgcatc
     2101 gaagtttccg gcgaacgcgt cgaggaggcc gagcttcagc ttgagctgag ccaaggagac
     2161 gagattccca acgctgatcg tgaggagtct ctcgccggcc gccatcgcct cggaaaccgg
     2221 cgcgggtccc cagatcaatc cgtacgaaac ggcggagacg atgtgcgcca gctcgaattt
     2281 ttcgggagtc tcaagactgt gctgaagcgc ggactccgcc gcactcgccg acgcgccaca
     2341 ctcgaaaagg ttccaataga actcggagag gccccgatag atcttgacca acgtccgatg
     2401 gtcgccctgc gcttcgaaga tcggaagtag ttcgcggaag gacttctcga tcgtgggatc
     2461 agttgaggtc agcgacttcg cgaacacctt catcaggtcg gctctcgccg acaccacagc
     2521 atcgcccaat tgcggagccg tttcttggat cagcttgacc atctcgagtg cttcacgcgt
     2581 gcgggcgaga tccaacaggc agtccgtcag ctcgatcgag tcgtcaaacc aagccgggcc
     2641 gagcaccaaa cgcagatcca gtgccctgcg gaagaggtcg gccgccgagg tggttgcccc
     2701 tctcgccctc tcgccccggc ccgcggcatg aagatggttc gcggccttct cggccacaga
     2761 gccgttgcgc ggatcgtcag gcacagcctc cttgcgcaat ctgaaggctc gctccaagtg
     2821 atatcccata aagtcggggc catagccttg aacttccgcg tacgcagcgg ctcgttcatg
     2881 cagctccgcg agccgctcct tcttgcattg aaggtacgca gcctcccgta acagagcgtt
     2941 tcggatcctg aatgtgctcg agccggcagc cgttgatgaa tcctcaatga aaccgctgga
     3001 gcgaagatcg gccagacaac tcaagacatc ggaagactct cgatcgggaa gcaagccctt
     3061 gagttgatcg acatcgaagt cgacactcca cgcggcagca gcttcga
//
LOCUS       DATMED010000067         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2357447, whole genome shotgun sequence.
ACCESSION   DATMED010000067 DATMED010000000
VERSION     DATMED010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357447"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="VNP73_01745"
     CDS             <1..2181
                     /locus_tag="VNP73_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWL64670.1"
                     /translation="GTDTVLDEEAGDVLTDCEISGAPPIVVNVTSDEEDTDLEDTDCD
                     VDAEETGEQCTLRAALQVANSQGGENEINFAIEPAGQATIAPEAELPAITEGVEIHGG
                     TQPGAPTGEPGVIVDGSQIEAGPRRAPQIFTLRISNGNGTILEQLGFTGSLASAIGVD
                     LDAQFTTLDGLLVGLTDMFASATTPNALDGIYVEGSDTTIRDSVISGNGRHGVHLAGS
                     SRFTKLINNTIGPLGPLEGLASLGPITNEGAGIAIAGGSSENVIGGATFAEGNTIGRN
                     GIGVLMDEAGRANEVTSNEIGAPHAGGLAVELLANEGEGIKVTDTRLDEGDELEITTN
                     TIANNGGDGIRMEDSTGADITGNALGALMSETDCTHVLIDNELRQIHVEVAEDVERSF
                     GIPPKRFLGGGGVNLGKADGQLLEALGGNLICDENGEAIGFAGILTAQIEANVIFGGA
                     MLENLEGMFSENIVSDSEGAGVELGKGTEVELAENLFVDNAGRPIVLRKGDAQAVPDP
                     LDPDKGPNQLQNYPLLGFFQTGPTQSTSRFSLLAAKKGQFRLEFFGADSCSPEGRANV
                     HEFLGSEVVETSPAGLVNGVATLPAGAEAYAFVTTTATRLDVPNPMTSEVGPCIATIP
                     ADDSGFGLTLLDPFVKKGKVKTMADCVGSTACAGEAEITDRDGSASYGSAEIEVAAGD
                     AKQIKIKANRKLERLLKKKGRAEVILTSTTDEVRSTALVLVLKN"
     gene            complement(2191..>2995)
                     /locus_tag="VNP73_01750"
     CDS             complement(2191..>2995)
                     /locus_tag="VNP73_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWL64671.1"
                     /translation="ALLGNLVVLPIGGADMPVVISLLNAATGLSAAAAGFSLDNVSLI
                     VAGTLVGSSGTILTMEMATAMNRSIPNILFAGFGGAPAASGAPGEERPHTSIGAQDAA
                     IKMAYADSVVIVPGYGLAVAQAQHAVKEMANQLEKRGVDVSYAIHPVAGRMPGHMNVL
                     LAEADVPYEKLREMEDINPEMPRVDVAVIIGANDVTNPAAKNDPDSPIAGMPIIEANE
                     AKQVIVIKRSMSPGFAGIDNDLFYLPNTDMLFADAKAAAGEIAAAIEDL"
BASE COUNT          562 a          939 c         1022 g          472 t
ORIGIN      
        1 ggcaccgaca cggttctcga cgaggaggca ggggacgtac tgaccgattg cgagatctcg
       61 ggcgcgccgc cgatcgtcgt caacgtcacc tcggacgagg aagacaccga cctcgaggac
      121 acggactgcg atgtcgatgc ggaagagacc ggtgagcaat gcacgctgcg cgccgcgctc
      181 caggtggcga acagccaagg aggcgagaac gagatcaact tcgcgatcga gccggccggc
      241 caggcgacca tcgcgccgga agcggagctg cccgcgatca cagagggcgt cgagatccac
      301 ggcggcacgc aacccggggc tccgacgggc gagccggggg tcatcgtcga cggttctcag
      361 atcgaggcag gaccgcgccg cgcgcctcag atcttcacgc tcaggatctc gaacggaaac
      421 ggcaccatcc tcgagcagct cggattcacc ggtagcctcg cgtcggcgat cggcgtcgac
      481 ctcgacgcgc agttcacgac cctcgacggc ctcctggtcg ggctcaccga catgttcgcg
      541 agcgctacga ctccgaacgc tctcgacggc atctacgtcg agggttccga caccaccatc
      601 cgcgacagcg tgatctccgg caacggccgc cacggtgtcc acctcgcggg ctcgtcgcga
      661 ttcacgaagt tgatcaacaa cacgatcggg cccctcggcc ctctcgaagg cctggcatcc
      721 ctcgggccga tcacgaacga gggtgcgggc atcgcgattg ccggtggctc ttccgagaac
      781 gtgatcgggg gcgcgacgtt cgccgagggc aacacgatcg ggcgcaacgg gatcggcgtc
      841 ctaatggacg aagccgggcg tgcgaacgag gtcacctcga acgagatcgg agcaccccac
      901 gccggtgggc tcgccgttga gctcctggcc aacgagggcg aggggatcaa ggtcacggac
      961 acccgtctcg acgaggggga cgagctggag atcaccacga acacgatcgc caacaacggg
     1021 ggcgacggga tccggatgga agacagcacc ggcgcggaca tcactgggaa cgcccttggg
     1081 gccctcatga gcgaaacgga ttgcacacat gtgctgatcg acaacgagct gcgccagatt
     1141 cacgtcgagg tggccgagga cgtcgagcgc agcttcggca tcccgccgaa gcgattcctg
     1201 ggcggcgggg gcgtcaacct agggaaagcc gatgggcagt tgcttgaggc cctggggggc
     1261 aacttgatct gcgatgagaa cggagaggcc attggtttcg ccggcatcct gactgcccag
     1321 atcgaagcga atgtcatctt cggcggtgcc atgctggaga acctcgaggg catgttcagc
     1381 gagaacatcg tctccgacag cgaaggcgcg ggggtagagc tcgggaaagg cactgaggtc
     1441 gagcttgccg agaacctctt cgtcgataat gccggccgtc cgatcgtgct caggaagggc
     1501 gacgcgcagg cagtgccgga tccactcgat ccagacaagg gccccaacca gctccagaac
     1561 tatccgctct tgggcttctt ccagacgggg ccgacgcaat ccacatctcg cttctccctg
     1621 ttggccgcca agaaggggca gtttcgactt gagttcttcg gcgccgattc gtgctcgcct
     1681 gaaggtcgag cgaatgtgca cgagttcctg ggctcggagg tcgtcgagac gagtccggca
     1741 ggtttggtga acggggtggc gacccttccc gcgggagccg aggcgtacgc gttcgtcacg
     1801 acgacggcaa cgaggctgga cgtccccaat ccgatgacct cagaggtcgg cccgtgtatc
     1861 gcgaccattc cggcggacga ctccgggttc ggcttgacgc tgctggaccc gttcgtgaag
     1921 aagggaaagg ttaagacgat ggccgactgc gtcggatcta ccgcctgcgc aggggaagcg
     1981 gagatcaccg atcgcgatgg gtccgccagc tacgggagcg cggaaatcga ggtcgcggcc
     2041 ggtgacgcta agcagatcaa gatcaaggca aaccgaaagc tcgagcggct gctcaagaag
     2101 aagggcagag ctgaggtcat cttgacttcg acgaccgatg aggtccgcag tacggcgctc
     2161 gtcctcgtcc tgaagaactg agggcgggga ctagaggtct tcgatcgccg cggcgatctc
     2221 gcccgccgcg gccttcgcgt cggcgaagag catgtcggtg ttcggaaggt agaagaggtc
     2281 gttgtcgatt ccggcgaagc ccggcgacat cgagcgcttg atgacgatga cctgctttgc
     2341 ctcgttcgcc tcgatgatcg gcatgccggc gatcggcgag tcggggtcgt tcttggccgc
     2401 cgggttggtg acgtcgttcg cgccgataat gaccgcgacg tccacgcgcg gcatctccgg
     2461 gttgatgtcc tccatctcgc gaagcttctc gtagggcacg tcggcctctg ccaagagcac
     2521 gttcatgtgg ccgggcatgc gccccgcgac cgggtggatc gcatagctca cgtccacccc
     2581 gcgcttctcg agctggttcg ccatctcctt gaccgcgtgc tgcgcctgcg ccacggcgag
     2641 gccgtagccc gggacgatca cgacggagtc ggcgtatgcc atcttgatcg ccgcgtcctg
     2701 cgcgccgatc gaggtgtgcg ggcgctcctc gccgggagct ccggatgcgg ccggtgcccc
     2761 gccgaagccc gcgaacaaga tgttcgggat cgaccggttc atcgcggtcg ccatctccat
     2821 cgtgaggatc gttcccgagg agccgacgag cgtgccggcg acgatcagcg aaacgttgtc
     2881 gagcgagaaa ccggccgctg ccgccgagag cccggtcgcg gcgttgagga gagagatcac
     2941 gaccggcata tccgcgccgc cgatcgggag gaccaccagg ttgccgagga gcgcc
//
LOCUS       DATMED010000068         4499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3889854, whole genome shotgun sequence.
ACCESSION   DATMED010000068 DATMED010000000
VERSION     DATMED010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3889854"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..699)
                     /locus_tag="VNP73_01755"
     CDS             complement(70..699)
                     /locus_tag="VNP73_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL64672.1"
                     /translation="MIRVVVVDDHPVVREGLAAVLADEPDFTIVGQADTGEKAIADLP
                     NSLPDVVVVDLRLPGISGTETCQELKRVRPSTRVIVLTSFPHDGAIMNAFVAGANGFL
                     LKESDPSVLRQAVREVAKGETYVDPRIAAKLVAFATKGRRTKGPFGLTLQEMRVIEKL
                     PKGMTNREIGRELGISEDTVKTHLRHAMYKIKAKDRAEAAAFALREGLA"
     gene            complement(696..2786)
                     /locus_tag="VNP73_01760"
     CDS             complement(696..2786)
                     /locus_tag="VNP73_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWL64673.1"
                     /translation="MLTSLAPVLAAERSAPDNAGRVDRRLLEQVGLALNSTLELSEVL
                     HRLTAVTLEASGADRCSIFLLEGHQLHPATAIGELRNEDLLTAFRSMGPVDLNTIPLA
                     WDLLAAGRAVPIEDALGSPLIPDPWVERFSLRSLVLVPLFAVTEPCGLLTLDYQFPRS
                     FSEEELRLYEAIGSYAGVAVRNAHLFETTRRRSQLNEGLVQASTQLVSPISRSEVLSR
                     LIDSYTGLFDARLCALGLVDAEHSKVQVMATRGTRKFEESISVNDIPERIHDKLRANW
                     NETKAVLLDDDPWMADFLGGRDAGAHKYVLIPLRVDNLRGGVLLGFPAHVRLNDEELS
                     AAEALGALSSASLERSLLLVRLERQVNRLEILHALGDALVERADAEGLKKRLNELLSH
                     HDIEVDALVFRDRSIASRLKGEAPTTQEREAWRSRKAFVRLSDGTLSVPMRLGKRLVG
                     AIRVRPVDIVDEEISFVEALAHGVAEVASRGALRATVEEAARESAVSAERGRIAADLH
                     DTAGQLFVAIGLFSRREAEHLPSDSPWATKFDRLAELADTGKWEISRAIQALAFFPEA
                     RRGITPSLRALARSFESDSGIETIVETPEKTSRLSPRIERALYRVAHEALSNAWRHSR
                     CQLVRIAIEFGPSEVMLRVFDDGVGVRRVGTLAGTGIAGMRRSLDEVGGSLKLRPLKP
                     HGTLVEARVKKESR"
     gene            2941..3621
                     /locus_tag="VNP73_01765"
     CDS             2941..3621
                     /locus_tag="VNP73_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64674.1"
                     /translation="MSPIQFETLRAMVHVWTDSATSEAAMRSWACRQEPGPDTALGEP
                     GPSSLEVQRSERSQLWFAPPARWRFESRAEGSEIPFIQIVNGSKWWLYNPIGRDSVVK
                     AGEDSPSNRFVSEYPPFAAMWDLDLLDESLVRVGLAEETYVGRHCLRERATPRVDLAD
                     DEYGGIWPGATEHELLIDAEHQVVIRWIAWYQSRFYGGVELEAVAFNDVFDPSLFDAP
                     AEERLRIR"
     gene            3874..>4499
                     /locus_tag="VNP73_01770"
     CDS             3874..>4499
                     /locus_tag="VNP73_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWL64675.1"
                     /translation="MLRRRTSDDCMAEDIAQETLVKALTRLDTLDLTLPLWPWLKTIA
                     TNLLIDHARRSGRELMTDDPVGATGVEFSPEDDDGTLLQAIENLPQKQQVAVSLRYLQ
                     GWKTTEAANFLGMGRPAFEQLLFRARRKLRTEYGRLQESALGLALLPLRWSRRLAWRI
                     SQQTRDANGFGPTTLIGSDAAVQLASGLLALATAIGGIAPVTDTAPLPT"
BASE COUNT          827 a         1405 c         1427 g          840 t
ORIGIN      
        1 acccctcacc gtgcaatgcg ggtgaaaagt cactctccga aaggggcact aggcgaaagt
       61 ggctcaccct catgccagtc cctccctcag tgcgaaggcg gcagcctcgg ctcgatcctt
      121 agccttgatc ttgtacatcg cgtgtctcag atgggtcttg acggtgtcct cggagatccc
      181 gagctctcta ccgatctccc gattggtcat gcccttcgga agcttctcga tgactcgcat
      241 ctcctggagc gtcaggccga acggtccttt ggtacgacgc cctttggtcg cgaacgcaac
      301 gagcttggcg gcgatccggg gatcgacata ggtctcgccc ttagcgactt ctcgcaccgc
      361 ctggcgcagc actgaagggt cggactcttt gaggaggaat ccgttggctc cggcaacgaa
      421 tgcgttcatg atcgccccgt cgtgagggaa gctggtcagc acgatcacgc gtgtcgaggg
      481 ccggaccctc ttcagctcct gacaggtctc ggtcccgctg atccccggca gcctgaggtc
      541 gaccacgacg acgtccggaa gcgagttagg caggtcggca attgccttct cgccggtatc
      601 ggcctgacct acgatcgtga agtccggctc gtctgcgagc acagcggcca agccctcgcg
      661 tacgaccggg tgatcgtcaa ctacaaccac tcggatcacc gggattcctt cttgacgcgg
      721 gcctcgacga gagtgccgtg tggcttcagg ggcctgagct tcagtgaccc gccgacctcg
      781 tccagtgacc tgcgcatgcc ggcgatgccg gtgccggcca gagttccgac ccgtctgacc
      841 ccaaccccgt cgtcgaacac gcgcaacatg acttcgctcg ggccaaactc gatcgcgatc
      901 cgaacgagct ggcagcgcga gtgacgccac gcgttcgata gagcttcatg ggctacccgg
      961 tagagagccc gctcgatccg gggagaaagg cgactcgtct tctcaggcgt ctcaacgatc
     1021 gtttcgatcc cgctgtcgga ctcgaacgat cgtgccagtg cacgcaacga cggagtgatc
     1081 ccccggcgcg cttcggggaa gaaggcgagg gcctggatgg ccctgcttat ctcccatttg
     1141 ccggtgtcgg cgagctcggc tagtcggtcg aacttggttg cccatgggga atcgctcgga
     1201 aggtgctcgg cttcacgccg cgagaagaga ccgatcgcga cgaagagttg tcccgcggtg
     1261 tcgtgaaggt cggctgctat acggccacgt tcggcggaga cggcactctc gcgtgccgct
     1321 tcctccacgg tcgcccgtag agcgccgcga cttgcgacct cggcaacacc gtgcgcaagc
     1381 gcctcgacga aggagatttc ctcgtcgacg atgtcgaccg gtcgaaccct gatcgcaccg
     1441 accaatcgct tgcccaaacg catgggaacc gacaaggtcc cgtccgacag ccggacgaag
     1501 gctttcctgc ttcgccacgc ctcacgctcc tgggtggtcg gggcctcgcc cttgaggcgg
     1561 gaggcgatcg aacggtcgcg gaacacgagt gcgtcgacct cgatgtcgtg atgggagaga
     1621 agttcgttga ggcgcttctt gaggccctcc gcgtcggctc gttcaacgag agcgtctccc
     1681 aacgcgtgaa ggatctccag ccgattcacc tgtcgctcga gtcgaacgag gagaaggctg
     1741 cgctcgagtg acgcggagga cagagccccg agggcctcgg ccgccgacag ctcttcgtcg
     1801 ttgagccgca catgggccgg gaatccgaga agcactcccc cacgcagatt gtcgaccctg
     1861 agtgggatca gaacgtactt atgtgcgccc gcgtcgcggc ccccgaggaa gtcggccatc
     1921 caggggtcgt cgtcgagcag gacggccttc gtctcgttcc agttcgcacg cagcttgtcg
     1981 tggatccgct cgggtatgtc gttcaccgag atcgactctt cgaacttcct cgtcccgcgc
     2041 gtcgccatga cctgcacctt ggagtgctcg gcgtcgacga ggccgagcgc gcacagccgc
     2101 gcgtcgaaca gtcccgtgta cgaatcgatc aagcgagaca gaacctcgct tcgagagatc
     2161 ggcgacacga gttgcgtcga cgcttgcacc aggccctcgt tcaactggga acggcgccga
     2221 gtggtctcga acaggtgggc gttgcgaacc gcgacaccgg cgtacgaacc gatcgcctcg
     2281 tagagacgaa gttcttcctc cgagaacgag cgcgggaact gatagtcgag ggtgagaagg
     2341 ccacacggct cggttacggc gaacagaggg acgagaacga gtgagcgaag actgaatcgc
     2401 tctacccacg gatccgggat caggggggag ccgagcgcgt cctcgatcgg aacggcacgc
     2461 cctgccgcca agaggtccca agccaagggg atcgtgttga gatcaaccgg ccccatgctg
     2521 cggaatgccg tcaggaggtc ttcgttcctc agctcaccga tcgcagtggc gggatggagt
     2581 tggtggccct ccaggaggaa gatgctgcag cggtcggcgc cgctggcttc gagggtgact
     2641 gccgtcagcc ggtggaggac ctccgagagc tcgagcgtcg agttgagggc gagaccgacc
     2701 tgctcgagaa gacgacggtc aacccgcccc gcgttgtcgg gggccgagcg ttcggcggct
     2761 aggacggggg ccaggctggt cagcatcaac tagtgacacg aaccagggca ggcaaaactt
     2821 acgcggcctc taaaaaatct ctggcacttc tctgccctga aatggctctg tgactcacct
     2881 gatcaggtga acggcctccc ttcgctatct tcaggcgatg ctggcggcag aggggggtac
     2941 gtgagtccta ttcagttcga gactctgagg gccatggtcc acgtgtggac cgattcggcg
     3001 acctcggagg cggcgatgcg ctcgtgggca tgccggcaag agccgggccc agacaccgcg
     3061 ctgggcgagc ccggcccgag ctcactcgag gtccaacgca gcgaacgctc gcagctctgg
     3121 ttcgctcccc ccgctcggtg gaggttcgag tccagggccg aagggtccga gatccccttc
     3181 atccagatcg tcaacgggtc gaagtggtgg ctctacaacc cgatcggacg tgactctgtc
     3241 gtcaaggcgg gtgaagactc tccctcgaac cggttcgtct cggaatatcc accgtttgca
     3301 gcgatgtggg atctagatct gctcgacgaa tcgctcgtcc gtgtggggct cgctgaagag
     3361 acgtacgtcg gtcgtcattg cctgagggag agggctacgc ctcgggtgga cctcgcggat
     3421 gacgaatacg gcgggatctg gccgggagcg acggagcacg aacttctgat cgacgccgag
     3481 caccaagtcg tgatccgatg gatcgcctgg taccaatccc ggttctacgg cggcgtcgag
     3541 ctggaggccg tcgcgttcaa cgacgtgttt gatccgagcc tgttcgacgc gccggccgag
     3601 gaacgccttc gtatccgatg aaccactcac tcacccgttt gggtgaggta gtcacccttt
     3661 tttggacttt gtggaggttt ctacctaaaa tccggagaac tttgaaccaa ccggattgct
     3721 gcgatccggg ggttttcaag cgggtgggtg gaatctctta tgagcctcag actggctgag
     3781 gcgccacaag gatctctcgt tcctgacgtc ctcgacgttc gggacgaagg cttcgaggtc
     3841 ctctacaggc gctactttcc tcggcttgtc cggatgcttc ggcgccggac gagcgatgac
     3901 tgtatggccg aagacatagc acaagaaaca ctcgtgaagg cgctgacgcg tctcgacacc
     3961 ctcgatctca ccctcccctt gtggccatgg ttgaaaacga tcgcaacgaa tctgctgatc
     4021 gatcacgcac gacgttcggg acgcgagttg atgacggacg atcccgtcgg cgcgaccggc
     4081 gtcgagttct cgcccgagga cgacgacgga acccttctac aggcgatcga gaaccttccc
     4141 cagaaacagc aggtcgccgt gtcgcttcgt tacctccaag gttggaagac aaccgaagcg
     4201 gcgaacttcc tcggcatggg ccgtccggcg ttcgagcagc tgttgttccg cgcgcgtcgc
     4261 aagcttcgga ccgagtacgg acgcttgcag gaaagcgcgc tcggtcttgc gctgcttcca
     4321 cttcgctggt cccgtcggtt ggcatggcgg atctcgcagc agacacgtga tgcgaatggt
     4381 tttggaccca ccacgcttat cgggtccgat gcggccgtgc aacttgcgag cggactcttg
     4441 gcgttggcaa cagcgatcgg aggcatcgct ccggtcaccg acaccgcccc cctaccgac
//
LOCUS       DATMED010000069         5096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2358412, whole genome shotgun sequence.
ACCESSION   DATMED010000069 DATMED010000000
VERSION     DATMED010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358412"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VNP73_01775"
     CDS             <1..250
                     /locus_tag="VNP73_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64676.1"
                     /translation="TNCFRSLGQYIRALKPKIYTPNHHDNFVVGAGDNARDMEPQVRE
                     EIQRIPKRIRPQLVYTYDPDAYLRPDLFTYDPADPRWR"
     gene            complement(247..1386)
                     /locus_tag="VNP73_01780"
     CDS             complement(247..1386)
                     /locus_tag="VNP73_01780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWL64677.1"
                     /translation="MTGATPLEEVLLERIRTQGAQPFAAFMSTALYHPKFGYYAGGEQ
                     RTGWRGHYLTSAELDPAFGALWTEAFERMWVACGRPNRFDLIEVGPGEGGFVAAVLET
                     ASGEFADALQVTLVERVARVQERQRQRLTGITNVGWVSSLDEVGPIDAGCIFANEVID
                     NLPVHLVEVRDGALREICVEEEDRRLLFTRRPPSNPELENFLQRAGVVLGEGHTYEVS
                     LTAEALVSRAATMIGCGALVLTDYGAEAGSLAERTDGTLLCYSDTGVDTEPLERVGEK
                     DLTVHANWTTLRKAAEAGALTVAGPITQRDVLRSLGSRAQDEELKSEHDRALAERRGS
                     AAMKALSRRQALGALTDPQGLGGLGVLIATRGFEPGLVTEERGRG"
     gene            complement(1383..1691)
                     /locus_tag="VNP73_01785"
     CDS             complement(1383..1691)
                     /locus_tag="VNP73_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64678.1"
                     /translation="MESVEQVKTFLRGQNLEQVDRTDEAIELYETCVGAGFDATGPYD
                     RLITIYSHRAQHGDVIRVAELALKNVQTYADKKAFYERMKSEAAKAAAKVPPAAKKRS
                     "
     gene            1760..2794
                     /locus_tag="VNP73_01790"
     CDS             1760..2794
                     /locus_tag="VNP73_01790"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWL64679.1"
                     /translation="MADLRSTAKALVAEGKGILAADESFPTIEKRFKSIQIENSEENR
                     RGYRDMLFTTPGLGDYISGVILFDETIRQSASDGTPFADLLDKQGSIPGIKVDKGAKD
                     LAFFSGEKVTEGLDGLRERCKEYVVLGARFTKWRAVITIGEGGGGSGRIPSDFCIHAN
                     AHALARYAALSQEAGMVPIVEPEVLMDGGHSIDDCLEATRRTLTELFDQLGRHNVEIP
                     GTLLKTNMVVSGKENAQQADLQEVASKTIDVLKQTVPDDLPGVVFLSGGMSDEDATAR
                     LNEMNKIGGFPWELSFSYGRALQAPSLDAWKGEASNVEAGQKALFHRAKMNGLARSGS
                     YKPEMENEAA"
     gene            2959..4059
                     /locus_tag="VNP73_01795"
     CDS             2959..4059
                     /locus_tag="VNP73_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64680.1"
                     /translation="MSTVVPGASEFRQRQALLFDRMEDLSLTSYELRAGWHRFGDLVR
                     PKDRHKYPKADAVSLPVTEEYYSLEGIDPRPAAEDLFYTYVRMGEEWFIAEDTDLDDL
                     TMYSSRHLWDLGPVDVLTASDFVLFSHPCATGGLCGDSGLLADARSAMGRVTKYWNDV
                     PERVAILVPSDQEVVERMIQATFELDNFVAFAYSSEDLSEGLDYTGNRIILNPPAFGG
                     RSTDSSRTILAHELLHIVTRDASGPFIPVFVEEGFAEYVGYDANRSSLSFLSGEIAAG
                     NFDRQIPEDFEFLIGSGTEIFRSYQESESAVFFFIERWGLDRFKRFYRRLGRIEIAPG
                     TVRYWLDKTLRRTIGMDLDGFEKAWADSIASS"
     gene            4056..>5096
                     /locus_tag="VNP73_01800"
     CDS             4056..>5096
                     /locus_tag="VNP73_01800"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWL64681.1"
                     /translation="MSNTAVADGKKDGFALAKDAEAAGAVAMVVAGKERDLSFVPDDS
                     AEVSFVTTESDEGRYIIRHSTAHVMAQAVLRLFPDAKYSIGPPIANGFYYDFDIERPF
                     TPEDLEAIEAEMKKIVKENQRFERKEISREEGLTLFKDQPYKIEIIEGVDLGDEAAEK
                     TDAGAVGEVLSIYSNRAADADEPAFVDLCRGPHLPSTSRIKAFKLLRSAGAYWRGDET
                     KPMLQRIYGTAWESKQALEDHLHRLEEAEKRDHRKLGRDLELFTWDEQVGPAIALWLP
                     KGAVVRQQLEDLIRGMVIERGYDPVYTPHIGKASLWETSGHLGYYKENMFPGMELEGA
                     EYYLKPMNCPFHI"
BASE COUNT          964 a         1621 c         1601 g          910 t
ORIGIN      
        1 gacgaactgt ttccgcagcc tcggccagta catacgggct ctgaagccga agatctacac
       61 cccgaatcat cacgacaact tcgtggtcgg tgccggtgac aatgcacgcg acatggaacc
      121 ccaggtgcgt gaagagatcc aacgcatccc caagcggatc cggcctcaac tggtctacac
      181 gtacgatccc gatgcatacc tacgaccgga tctcttcact tacgacccgg ccgatcctcg
      241 ctggcgctag cctcgcccgc gttcttccgt caccaacccc ggctcgaagc cacgcgtggc
      301 gataagcacg cccaggccac cgagaccctg gggatcggtc aaggctccca gcgcctgacg
      361 gcgagataac gccttcatcg cagcgcttcc ccggcgttct gcgagggctc gatcgtgttc
      421 ggacttgagc tcctcgtctt gtgcacgcga gccgagagag cgcagcacgt cgcgctgcgt
      481 gatgggcccc gcaacggtaa gagcgcccgc ctctgcagct ttccgaagcg tcgtccagtt
      541 cgcgtggacg gtgaggtcct tctcgccgac gcgctcgagc ggctcggtat ccacacctgt
      601 gtcggagtag cacaacaatg ttccatccgt ccgctcggca agggagccgg cctcggctcc
      661 gtagtcggtc agcacgagag caccgcagcc gatcatcgtt gcagcgcggg agaccagtgc
      721 ctcggcggtc aacgaaacct cataggtgtg cccctcgccg agcacaactc ctgctcgctg
      781 taggaagttc tccaactccg gattggaggg agggcgtctc gtgaacaaca gccttctatc
      841 ctcctcctcg acgcagatct cccgaagcgc tccgtcgcgg acctcgacga gatgcaccgg
      901 caggttgtcg atcacttcgt tggcgaagat gcaccccgca tcgatgggac caacctcgtc
      961 cagggatgag acccaaccaa cgttcgtgat gccggtaagt cgctgtcgtt ggcgctcttg
     1021 aacgcgagcc actcgctcga cgagcgttac ctgcaacgcg tctgcgaact cacccgatgc
     1081 cgtctccaac acggccgcga cgaaaccccc ctctccggga ccaacttcga tcagatcgaa
     1141 gcggttgggc cgcccgcacg cgacccacat gcgctcgaat gcttcggtcc acaacgcgcc
     1201 gaatgcggga tcgagctcgg ccgaggttag gtagtgcccc cgccaaccgg tccgctgctc
     1261 gccgccggcg tagtagccga acttggggtg atacagagcc gtggacatga acgccgcgaa
     1321 cggttgtgca ccttgcgtcc ggatgcgctc gagcagaacc tcttcgagcg gagtggcccc
     1381 ggtcacgaac gcttcttggc cgcgggcggg actttggccg cggctttggc tgcctccgac
     1441 ttcatccgct cgtagaaagc cttcttgtcg gcgtaggtct gcacgttctt gagagcgagc
     1501 tcggcgaccc ggatcacgtc tccgtgctgg gcccggtgcg agtagatggt gatcagacgg
     1561 tcgtagggcc ccgtcgcgtc gaagccggcc ccgacgcagg tctcgtacag ctcgatcgcc
     1621 tcgtcggtcc ggtccacctg ctcgaggttc tgcccccgca ggaaggtctt gacctgctcg
     1681 acgctctcca tctttagagc ctaggtagtc tttacggctc acgcttacac aaggcttagg
     1741 cgacagagga ggaccccaga tggcagacct tcgttccacc gcaaaggcgc tcgtggcaga
     1801 aggcaaaggg atcctggctg cggacgagag cttcccgacc atcgagaagc ggttcaagag
     1861 catccagatc gagaactccg aggagaaccg gcgtggctac cgcgacatgc tgttcaccac
     1921 gcccgggctg ggcgactaca tctccggcgt catcctgttc gacgagacga tccgacagtc
     1981 ggcgtccgac ggaaccccgt tcgcagatct gctcgacaag caggggtcga tccccgggat
     2041 caaggtcgac aagggcgcga aggatctggc gttcttctcc ggcgagaagg tgacagaggg
     2101 cctcgacggg cttcgtgaac gctgcaagga gtacgtggtc ctgggagccc gattcacgaa
     2161 atggcgtgcg gtgatcacga tcggagaggg cggaggcgga agcggacgca tcccctccga
     2221 cttctgcatc cacgcgaacg ctcatgccct ggcgcgctac gcggccctgt cgcaggaggc
     2281 ggggatggtt ccgatcgtcg agccggaggt gctgatggac ggtggtcact cgatcgacga
     2341 ttgcctcgag gcaacgaggc ggaccctcac cgagctgttc gaccaactgg gccgtcacaa
     2401 tgtcgagatc cctgggaccc tcttgaagac gaacatggtg gtgtccggca aggagaacgc
     2461 gcagcaagcc gacctgcagg aggttgcgtc caagacgatc gacgtactga agcagaccgt
     2521 gcccgacgat ctccctggtg tcgtgttcct ctcgggtggg atgagcgacg aggatgcgac
     2581 cgctcgcctc aacgagatga acaagatcgg cggttttccc tgggagctct cgttctccta
     2641 cggacgcgcc ctgcaggcac cttcgctcga cgcgtggaaa ggcgaggctt cgaacgtcga
     2701 agcggggcag aaggcgctgt tccaccgggc caagatgaat gggctggccc gctccggctc
     2761 gtacaagcct gagatggaga acgaagccgc ctgagcttca gccggcggtt cgccccggct
     2821 tgcgtcgcgg cagcggcgct cgtcgcgctc ggagtagttg cagaccccgg cgttgccgcg
     2881 cccggcgctc gctccgggat ccggtctctc ctcgaacgcc ggtcgacggc gatagtcgag
     2941 cgtgatcgcg atgcgttcat gtcgaccgtc gttcccggcg cgagcgagtt ccggcagcga
     3001 caggcgttgc tattcgaccg gatggaggac ctgtcgctga cttcgtacga gttgcgcgcc
     3061 ggatggcacc gtttcggaga tctcgtaaga cccaaggatc gccacaagta cccgaaggcc
     3121 gacgccgtga gcctccccgt gacggaggag tactactcgc tggagggaat cgacccccga
     3181 ccggcagccg aggacctctt ctacacctac gttcgaatgg gcgaggagtg gttcatcgca
     3241 gaggacaccg atctcgacga cctgacgatg tattcctccc gacacctgtg ggatctcggc
     3301 cccgtcgacg tcttgaccgc ctcggacttc gttctctttt cgcatccatg tgcaaccgga
     3361 ggattatgcg gcgattccgg cttgctggcc gatgcacgct ccgcgatggg tcgcgtgacg
     3421 aaatattgga acgacgttcc cgaacgcgtc gcgatcctcg tccccagcga ccaagaggtg
     3481 gttgaacgga tgatccaggc gacattcgag ttggacaact tcgttgcgtt cgcctactcg
     3541 agcgaggatc tgtccgaagg gctcgattac accggcaacc ggatcatcct taatccacct
     3601 gcgttcggag gccggtccac cgactcgtcg agaacgatcc tggctcacga actgctgcac
     3661 atcgtcaccc gcgacgcctc cggccccttc atcccggtgt tcgtcgagga gggtttcgcg
     3721 gagtacgtgg gttacgacgc caaccggagc tcgctgagtt tcctctccgg cgagatcgcg
     3781 gccgggaact tcgaccgcca gatccctgag gatttcgagt tcctgatcgg ttccggcacc
     3841 gagatcttcc gctcctacca ggaatcggaa tcggcggtct tcttcttcat cgagaggtgg
     3901 ggcctggaca ggttcaagcg tttctatcgt cggctcggcc gcatcgagat cgcgccgggg
     3961 acggtccgct attggctcga caagacgttg cgccggacga tcggcatgga cctggacggg
     4021 ttcgagaagg cgtgggccga tagcatcgcc tcatcatgag caacactgcc gtagcagacg
     4081 ggaagaagga cggtttcgcg ctcgccaagg acgcagaggc ggcgggggcc gttgcgatgg
     4141 tggttgccgg gaaggagcgc gacctctcgt tcgtgcccga tgactcggcc gaggtgtcct
     4201 tcgtaacgac cgaatcggac gaaggccgct acatcatccg acactcgacc gctcacgtga
     4261 tggcgcaggc ggtgttgcgt ctgttcccag acgccaagta ttcgatcgga ccgcccatcg
     4321 ccaacggctt ctattacgac ttcgacatcg agcggccgtt cacgcccgag gacctcgagg
     4381 cgatcgaagc cgagatgaag aagatcgtca aagagaacca gcgcttcgaa cgcaaggaga
     4441 tctcgcgcga ggaaggcctg accctgttca aggatcagcc ttacaagatc gagatcatcg
     4501 aaggggtcga cctcggagac gaagcggccg agaagaccga tgcaggagcg gtgggcgagg
     4561 tgctgtcgat ctactcgaac cgggccgccg atgcggacga acccgcgttc gtggacctct
     4621 gtcgcggccc gcacctcccg agcaccagtc gcataaaggc gttcaagttg ctgcgttctg
     4681 ccggcgccta ctggcgcggc gacgagacca aaccgatgtt gcagcggatc tacgggaccg
     4741 cgtgggagtc gaagcaggcg ctcgaggatc atctgcaccg gctcgaggaa gccgagaagc
     4801 gcgaccaccg gaagctgggt cgcgatctgg agctgttcac gtgggacgag caggtgggac
     4861 ccgcgatcgc actctggcta cccaagggtg cggtcgtccg gcagcagctc gaggacctga
     4921 tccgagggat ggtcatcgaa cgcgggtacg accctgttta cacgcctcac atcggcaagg
     4981 cgtctctgtg ggagacgagc ggacacctcg gctattacaa ggagaacatg ttccccggca
     5041 tggaactcga gggggccgag tactacctca agccgatgaa ctgccccttc cacatc
//
LOCUS       DATMED010000070         4719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1594177, whole genome shotgun sequence.
ACCESSION   DATMED010000070 DATMED010000000
VERSION     DATMED010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594177"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /gene="radA"
                     /locus_tag="VNP73_01805"
     CDS             complement(<1..1272)
                     /gene="radA"
                     /locus_tag="VNP73_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWL64682.1"
                     /translation="MSSFLCTACGHESLKWFGRCPSCGDWSSASSADPAPEGAEIVSL
                     QSLDDMPSRLSTGLAEVDRVLGGGLVLGEVVLLAGEPGVGKSTLVLQIVDGIARNKAR
                     AIIFSGEESAHQVGLRAARLGLATDKISFSPSNSLQAILEACRAEQPDLIVVDSIQTM
                     SDRDLEHAAGSVVQVRECAAALVAHAKAAGSAVLITGHVTKEGSVAGPKVLEHLVDAV
                     LSLEGERTGAMRLLRAQKNRFGSCDELGVFSMGERGLTTLEDPSALLLDDRAPGTPGS
                     IVYPSMDGSRPLLIELQALVERSALPQPRRVAIGIDQRRLTLVLGVLSKHLSLGLAEM
                     DVFMAAAGGLAVREPASDLAMSLALASAGRGWVIDERTVAFGEVGLGGEVRRVPGVVR
                     RLEEARRLGFDRALAPKGTAPCEGIEVVRVTR"
     gene            complement(1314..4193)
                     /locus_tag="VNP73_01810"
     CDS             complement(1314..4193)
                     /locus_tag="VNP73_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWL64683.1"
                     /translation="MLGPLVLTGILFLVLAIVAVRAPVARRLAFRNAIRRPTETALVI
                     AGSLLGTALITGSFIVGDTLDSSIRATAFNQLGPIDEVVVLQEANTAQDIADRLESED
                     DPNVDGVLTAVVVPASVATQAEGGRTRAEPESQLLELDFESARAFGDAPDTTGISGPT
                     PGEGEAAITEDLAETIGASEGDEIAVFLYGEDRELTVSRILPRTGVAGFWLGFESESP
                     NVFVSPGTVEEITRGKVPTGAIPPTATLFVSNTGDVEEGAALTSEVTTTIEGAIGDVP
                     ARVEDIKQQRLDDAETTGAEFSELFLGIGSFAIIAGILLLVNIFVMLAQERQSQLGML
                     RALGLRRADLVRVFAIEGTIYAAAASVLGSIVGIGVGWAIAKLAAPIFGGIDEFALQL
                     RFAMDLESIVIGFTLGAFITTMTILFTSIRISRVNIISAIRDLSQPPERKARRRTVII
                     GVAVSVITLAAFVASFGQRDAWAIAILGPPIVVYSLLPLAGRFMARRRAVLIASAFSL
                     AWGIFGDMITNKAFFESGEIFAFVLQGILLTFSAVVLLSQTAERFEKIIRFIAARNLP
                     IRLGMAYPIARRFRTGLTLGMYALVIFTMTFIAVLSTIFGGQVDTTIDRASGGWHIAA
                     SASGTNPPRLETLEAIDGVEMVAPLVSSNALFQTEGSPEPEDWAITGVDEVFVEGGPP
                     ALEERDTERYPTDRAVWEGILEDPGAVIVDEFFLQEGGGGPPTAVVQPGDTMTVIDPI
                     TGKEAERRVAGITSVDATFAGVFVSQDSTKEILGPRSVPSRFWVQAEGGESQANALAA
                     RLQGDLFRNGVEADSFRTLVEDFQQVNLQFLNLMQGYLALGLLVGIAGLGVLAVRSVR
                     ERRREIGVLRSLGFVPPQVRRSFLFESAFIALQGILIGSILAVITSAQLVGTESFGES
                     AVFEVPWTQLAILCGAALVASIIATAWPAQQASRIAPAVALRVAE"
     gene            complement(4201..>4719)
                     /locus_tag="VNP73_01815"
     CDS             complement(4201..>4719)
                     /locus_tag="VNP73_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL64684.1"
                     /translation="DDVDAGTVLVEGKDIHKMSDAARSRHRAAEMGFIFQSFNLIPVF
                     TSAENVELPLLLSGTASAEARKRAETTLEQVGLGNRTNHRPNELSGGEQQRVTIARAL
                     AGGPSIVWADEPTGNLDSETAGSIMELLRELNRNGLTLVLVTHDPLIGKTAQRVIRMR
                     DGAIESESPGGD"
BASE COUNT          947 a         1528 c         1448 g          796 t
ORIGIN      
        1 gcgcgttacg cgcacgacct cgataccttc gcacggcgcc gtgcccttcg gtgccagagc
       61 ccgatcgaag ccgagacgac gcgcttcctc gagccggcgc acgacgcccg gcacgcgtct
      121 gacctcgccc cccaacccca cttcgccaaa tgcaacggtt cgttcgtcta taacccatcc
      181 ccgtcccgcc gacgcgagcg caaggctcat cgcaagatcc gaggcgggct cgcgcacagc
      241 aagaccgccg gcggccgcca tgaagacatc catctcggcc aggcccagtg aaaggtgctt
      301 cgacaacacg ccgaggacaa gcgtcaatcg tctttgatcg ataccaattg cgacgcgccg
      361 cggctgtggc agagcagacc tctcgacgag agcttgcaac tcgataagca aaggacgcga
      421 tccgtccatg ctcgggtaaa cgatcgatcc gggcgttccg ggagccctgt cgtcgagaag
      481 tagagccgac gggtcctcga gggtggtcag cccccgctcg cccatcgaga acacaccgag
      541 ctcgtcgcaa gaaccgaacc ggttcttctg tgcgcgaagg agtcgcatcg cgccggtgcg
      601 ctcgccttct agcgacaaca ccgcgtcgac cagatgctcg agcaccttgg gccctgcgac
      661 gcttccctct ttggtgacgt gccccgttat caggacggca gatcccgcgg cctttgcatg
      721 agcgacgaga gcggcggcgc actcgcgcac ctggacgacc gatcccgccg cgtgttcgag
      781 atctcggtcg gacatcgttt ggatcgagtc gacgactatc aggtcgggct gctcggcgcg
      841 gcacgcttcg agaatcgcct gcaatgagtt cgagggcgag aaactgatct tgtcggtggc
      901 aagaccaagg cgcgcggccc tgaggccgac ctggtgagcc gattcctcgc cggagaagat
      961 gatcgcgcgg gccttgttgc gagcgatccc atccacgatc tgtaagacga gtgttgattt
     1021 ccccacgccg ggttcgccgg ccagaaggac aacttcgccg agaacgagac cgccaccgag
     1081 cacgcggtct acctcggcga gccccgtcga gagccgggat ggcatgtcgt ccagcgactg
     1141 cagagagacg atctccgcgc cctcgggggc aggatcggcg ctggacgcgc tactccaatc
     1201 accgcacgaa gggcaccggc cgaaccattt aagggactcg tggccgcagg ccgtacacag
     1261 gaacgagctc atgcaggaaa cgctagcgag ggcctacgac aaaaagttct tcgctactcg
     1321 gccacccgaa gagcgaccgc cggggcgatc cgggatgcct gctgggccgg ccaggcggta
     1381 gcgatgatcg aggcgaccag cgcggcaccg cacaggatcg ccagctgcgt ccatggaacc
     1441 tcgaagacag ccgattcgcc gaaggactcc gtgccgacca actgagccga ggtgatcacg
     1501 gcaagaatcg agccgatcag gatgccctgg agagcaatga aggccgactc gaacaggaac
     1561 gaccggcgga cctgcggggg aacgaaacca agtgagcgca gcacgccgat ctcccggcgc
     1621 ctctctcgta ccgaccgaac cgcaaggacg ccaagcccgg ctatgccgac gaggagacca
     1681 agcgcgagat acccctgcat gagattcaag aactggagat ttacctgctg aaagtcctcg
     1741 acgagcgttc ggaaactgtc cgcttcgacg ccatttcgga acagatcgcc ttgcagacgc
     1801 gcggcaagcg cgttggcctg cgattcaccg ccctcggctt gaacccagaa cctcgatgga
     1861 accgaacgcg ggccgaggat ctccttcgtc gagtcctggg acacgaacac gcccgcgaaa
     1921 gtcgcgtcga cactcgtgat gccggctacg cgccgctccg cttccttccc ggtgattggg
     1981 tcgatcaccg tcatcgtgtc cccgggttgc acgacggcgg tgggagggcc gcctccgcct
     2041 tcctgtaaga agaactcatc gacgatcacg gccccggggt cctcgaggat cccttcccag
     2101 accgcacgat ccgtcgggta ccgctcggta tctcgttcct cgagagccgg aggaccgccc
     2161 tcgacaaaga cctcgtcgac gccggtgatg gcccagtcct cgggttccgg gctcccctcc
     2221 gtctggaaca gcgcgttcga cgacacaaga ggggccacca tctctactcc gtcgatcgcc
     2281 tcgagggtct cgagtcgtgg agggtttgtt ccagaagcgc ttgcggcaat gtgccagcca
     2341 cccgaagcgc gatcgattgt cgtatcgacc tgccccccaa agatcgtcga taggacggcg
     2401 atgaacgtca tcgtgaaaat aacgagggca tacataccga gcgtgagtcc ggtgcgaaag
     2461 cgtctcgcga tcgggtaggc cattccgaga cggatcggga gattgcgggc tgcaatgaac
     2521 ctgatgatct tttcgaatct ctccgcagtc tggctcaaca agacaaccgc cgagaacgtg
     2581 aggagaatgc cctgcaagac gaaagcgaag atttcgcccg attcgaagaa cgccttgttg
     2641 gtgatcatgt cgccgaagat cccccacgca agggaaaacg cggaggctat gagaacggct
     2701 cgcctccgag ccatgaaacg ccccgctaga ggcagcaggg agtagacgac aatgggggga
     2761 ccgaggatcg cgattgccca cgcatcccgt tgtccgaagc tcgccacgaa cgcggcaagc
     2821 gtgattaccg agacggctac accgatgatc accgtgcgac ggcgagcctt cctctcggga
     2881 ggttgagaaa gatcgcggat cgcactgatt atgttcacgc gcgagatgcg aatgctcgtg
     2941 aataggatcg tcattgttgt aatgaacgcg ccgagagtga atccgatgac gatgctctcg
     3001 agatccattg cgaaccgaag ttgcaatgca aattcgtcga tcccaccgaa gatgggcgcg
     3061 gccagcttgg cgatggccca tccgactcct atgccgacga tcgagccgag aacgctggcg
     3121 gcggccgcgt agatcgtccc ctcgatcgca aacaccctta cgagatccgc tctgcgaaga
     3181 cccagggctc tgagcatccc gagctgactc tgacgttcct gagccagcat cacgaagatg
     3241 ttcaccagaa gaagaatccc ggcgatgatc gcgaacgagc cgatgccgag gaacagctcg
     3301 ctgaactccg ccccagttgt ctcggcatcg tcgaggcgct gttgctttat gtcttcgacg
     3361 cgagccggta cgtccccgat cgctccttcg atcgtcgtcg tgacctcaga ggtcaaggcc
     3421 gcaccctctt cgacgtcgcc ggtattggaa acgaacagcg ttgccgtcgg cgggatcgcg
     3481 ccggtcggaa ccttcccgcg ggtgatctcc tcaacggttc caggcgagac gaaaacgttg
     3541 ggtgactcgc tttcgaagcc gagccagaac cccgccacgc cggtccgtgg aaggatccgc
     3601 gacaccgtca gttcgcgatc ctcgccgtac aggaagacgg cgatctcatc accctcggat
     3661 gcaccgatcg tttcggcaag gtcctccgtg atggcggcct ccccctcgcc cggtgtgggg
     3721 ccagatatgc ccgtggtgtc tggcgcgtct ccgaacgctc tggcggattc gaagtcgagt
     3781 tccagcaact gagattccgg ctctgcccgc gtccggcctc cttccgcctg cgtcgcaacc
     3841 gacgcgggaa cgaccaccgc ggtgagcact ccgtcgacgt tgggatcgtc ctcgctctcg
     3901 agccgatcgg cgatgtcctg agccgtattg gcttcctgca gaacgacgac ctcgtcgata
     3961 ggcccgagct gattgaaggc ggtcgcgcgg atcgacgagt ccagggtgtc cccaacgatg
     4021 aagctgccgg tgatcaacgc cgttccgagc aagctccctg caatgaccag ggccgtttcg
     4081 gtagggcgac ggatcgcgtt gcgaaacgca agcctgcgag caaccggcgc acgaacggca
     4141 acgatggcca gaacgaggaa gaggattccc gttaagacga gggggccgag cacagcttgg
     4201 ctaatccccg cccggcgatt ctgattcgat ggctccgtcg cgcatccgaa tgacccgctg
     4261 ggcggtcttg ccgatgaggg ggtcgtgcgt gaccagaacg agcgtcagtc cattgcgatt
     4321 caactcgcga agcaactcca tgatcgatcc ggcggtctcg gagtcgaggt tcccggtcgg
     4381 ctcgtcggcc cagacgatcg acggaccgcc tgcaagggcc ctggcaatcg tcacccgctg
     4441 ttgctcgccg ccggaaagct cgttcggacg atggttcgtt cggttgccca gtcccacctg
     4501 ctcgagcgtc gtctcggcgc gcttacgagc ctcggcggat gcggttcccg aaagcagaag
     4561 cggcagctcg acgttctcgg ccgacgtgaa cacagggatc aggttgaacg attggaatat
     4621 gaaccccatc tctgcggccc ggtgccggct gcgagccgcg tcggacatct tgtggatgtc
     4681 cttgccctcg accagcacgg tgccggcatc gacatcgtc
//
LOCUS       DATMED010000071         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_825769, whole genome shotgun sequence.
ACCESSION   DATMED010000071 DATMED010000000
VERSION     DATMED010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825769"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..945)
                     /locus_tag="VNP73_01820"
     CDS             complement(145..945)
                     /locus_tag="VNP73_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWL64685.1"
                     /translation="MPEDSPDLLLERDGHVLTVVMNRPEAKNAFSAEMLVRMVDAWEE
                     IDRDDDVRVAILTGAGDTFCAGADLKTMFGGQTDDEWSERFQSDSDMAWRALLRHYQL
                     TKPLIAAVEGYAVAGGTEILQATDIRVAGEGAVFGLAEVTRGLFPLGGSTVRLQRQIG
                     YAAAMEVLLMGEHIPAPRALEMGLINRVVPDGQALTEARKIADKIARNAPLSVQAIKK
                     SVQETAALPEQEALKIELEIGLPVFGSEDAREGTKAFAEKREPNYKGK"
     gene            999..2204
                     /locus_tag="VNP73_01825"
     CDS             999..2204
                     /locus_tag="VNP73_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWL64686.1"
                     /translation="MAPETVEVDLYNPDNFVASVPHDIFDWLRANDPVHFHEEPDGPG
                     FWVLTRFDDVMHASREWSDFSSAHGTSIEDAQGGAELMMLNMDPPQHTKLRKLVSSGF
                     TPRMIARTEPHIREIAAEIVDAVAKRGECDFVTEVAAELPLQVIAEFIGIPLEDRHKI
                     FTWSNTMIGLDDPEYANSSIEKATEAAAEMFGYADTLATERRKTPRQDLVTKLIQAEV
                     DDDQLTDMEFDAFFLLLAVAGNETTRNLISGGMLALMENPDERRKLIEDQSKIPTAIE
                     EMLRMVTPVMHFRRTATKDVEIRGHQIKEGDKVVVWYISANRDADMFPNPHAFEGARN
                     PNEHIAFGGGGPHFCLGANLARLEIRVMFEEILKRLPDMELTDTPARLRSNFINGLKH
                     MPVRFTPER"
     gene            2218..>2498
                     /locus_tag="VNP73_01830"
     CDS             2218..>2498
                     /locus_tag="VNP73_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWL64687.1"
                     /translation="MRAKLDVPILEGRLVKLEPLSVDHAPDLSMSACEDRSSYGFTVV
                     PETDTVRDYLADQFERAEEGLVPFAQIRRADDRAVGCTAYWDPRYWPRR"
BASE COUNT          480 a          782 c          759 g          477 t
ORIGIN      
        1 ggggtggact gctttcgatt tctgctgcca gctcgttgag agcggcggcc gcgcgcagga
       61 gcccctcgtc tgagatgtcc ttggttgcag cggcctcgat cagtcgacgt tgagcagccg
      121 cgaggcgtgt gcgcggagga agggtcactt gcctttgtag ttcggttcgc gtttctcggc
      181 gaaggctttg gtcccctcgc gtgcgtcttc gctaccgaac accggaaggc cgatctcgag
      241 ctcgatcttc aaggcttcct gctcgggcag cgccgcggtt tcctgcaccg acttcttgat
      301 cgcttggacg gacagaggtg cgttgcgagc gatcttgtcg gcgatctttc gggcttcggt
      361 gagcgcttgc ccgtcgggca cgactcgatt gatgagtccc atctcgagtg ccctgggggc
      421 cgggatgtgc tcgcccataa gcaggacctc catcgcagcg gcataaccga tctggcgctg
      481 gagacggacc gttgaaccgc cgagcgggaa gaggccacgt gtgacttctg cgagtccgaa
      541 caccgcacct tctccggcga cccggatgtc ggttgcttgc aggatctcgg ttccgccggc
      601 aacggcatag ccctcgaccg ccgcgatcag aggcttggtc aactggtagt gacgcagcag
      661 tgcccgccac gccatgtccg agtcggactg gaaccgctca gaccactcgt cgtccgtctg
      721 tcctccgaac atcgtcttca ggtcggctcc cgcgcagaac gtgtctccgg ctcccgtgag
      781 tatcgccacg cgcacgtcgt cgtcccggtc gatctcctcc caggcatcga ccattcgaac
      841 gagcatctcg gcgctgaaag cattcttggc ttccggccgg ttcatgacga ccgtcaggac
      901 gtggccgtct cgttcgagga gtaggtcggg ggagtcttct ggcatcgcac gattagacca
      961 catttgcggc agaaatgaga acgtgttcta cttttcacat ggccccggag accgtagagg
     1021 ttgacctcta caaccccgac aacttcgtcg cctcggtccc gcacgacatc ttcgactggc
     1081 tgcgcgcgaa cgatcccgtt cactttcacg aggagcccga cggtcccggt ttctgggttc
     1141 tcactcgttt cgatgacgtg atgcacgcgt cccgggaatg gtcggacttc tcctccgcgc
     1201 acggcacgag catcgaggac gcgcaagggg gggccgagct gatgatgctc aacatggatc
     1261 ccccgcagca cacgaaactc aggaaactcg tgagcagcgg cttcaccccg cggatgatcg
     1321 cgcgcaccga acctcacatc cgcgagatcg cggcggagat cgtcgacgcg gtggctaaga
     1381 ggggggagtg cgacttcgtc accgaagttg cagccgagct ccccctccag gtgatcgccg
     1441 agttcattgg catcccgctg gaagaccggc acaagatctt cacctggagc aacacgatga
     1501 tcgggctcga cgatccggaa tacgcaaaca gctcgatcga gaaggcgaca gaagccgcag
     1561 ccgagatgtt cggctacgcc gacacactcg cgaccgaacg cagaaagacg ccgcgccagg
     1621 atctcgtcac gaaactcatc caggcggagg tcgacgacga ccagctcacc gacatggagt
     1681 tcgatgcctt cttcctgctg cttgcagttg caggcaacga gacgacgcgc aacctcatct
     1741 ccggagggat gctggcgctg atggagaacc cggacgagcg tcggaagctg atcgaagatc
     1801 agtcaaagat ccccaccgcg atcgaagaga tgctgcgcat ggtgacgccg gtcatgcact
     1861 tccgtcgtac tgcgactaag gacgtcgaga tccgaggaca tcagatcaaa gagggcgaca
     1921 aggtcgtcgt ctggtacatc tcggcgaacc gcgatgcaga tatgttcccg aatccccacg
     1981 ctttcgaggg tgctcggaat ccgaacgagc acatcgcgtt cggcggggga ggcccacact
     2041 tctgcctggg tgcgaacctc gctcgcctgg agatccgagt catgttcgag gagatactga
     2101 agcgccttcc cgacatggaa ctcaccgaca ccccggcccg cttgcgctcg aacttcatca
     2161 acggcctgaa acacatgccg gtgaggttca cgcccgagcg ctagattccc ctccgtgatg
     2221 agggcgaaac tcgacgtacc catacttgag ggtcgtctag tcaaactgga gccgctgtcc
     2281 gtagatcacg cgccggatct gagtatgtcc gcctgtgagg atcgcagcag ctacggcttc
     2341 acggtcgttc ccgagactga cacggtcaga gactatctag cggaccaatt tgagcgagcc
     2401 gaagaaggcc tcgttccctt tgcgcagatc cgacgagccg acgaccgtgc tgtgggatgc
     2461 acggcgtatt gggaccctcg ctactggcct cgtcgaga
//
LOCUS       DATMED010000072         4037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3501315, whole genome shotgun sequence.
ACCESSION   DATMED010000072 DATMED010000000
VERSION     DATMED010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501315"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VNP73_01835"
     CDS             <1..594
                     /locus_tag="VNP73_01835"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWL64688.1"
                     /translation="WTGDARKREDVVAAVEAARKELGRIDGLVDIVGMAKYREALETS
                     DEDWDWSFDMVLRHAFLFSQLAGKVMADDGGGVMVFVASVSGLTGAPRHAAYGAAKAG
                     LISWVRSVAVELGPSQIRVNAVAPGVVLTPRVAAYLDERSREMQANNAPLRRVAVPED
                     IAAAILFLASDLASYISGQTLVVDGGVSAKFPYPMEL"
     gene            597..1586
                     /locus_tag="VNP73_01840"
     CDS             597..1586
                     /locus_tag="VNP73_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWL64689.1"
                     /translation="MDPASFLRGVAFPGNERVPYPRAKPEDAARLPLDTWSCARIPVG
                     VRLELIGDASEIEIAYRTETEELGYRGDGAGRTFAVWRDGRPVDCEKASLGEGTVRLS
                     LGETVDARAIVYVPEGMKPTILDVSSVGGTIEPAPSQPRWVAYGDSVLEGWTASEPAL
                     GWGHQAARDFGLDMVNMGYAGSARGEIASAEHVAELDADVISITHGTNCWARTPHSVG
                     LYREGLRAFLDIVRGGHPNTPIVVASPVIRPDAETTPNLLGASLGDLRRMMEEVVQDR
                     IAHGDADLTLVQGGELIDEGQLADGIHPNDEGHAAMAAALGPAIRSALDRGVS"
     gene            1586..2446
                     /locus_tag="VNP73_01845"
     CDS             1586..2446
                     /locus_tag="VNP73_01845"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWL64690.1"
                     /translation="MPRRAPAVERSIAVLNLLAQHPGERYTLSEIARDLSLNKATLHA
                     ILWALSEAGYLVRDPEEKSYALGPALVAVGNAALSSFSAVEVARPHMDALAEELDLDV
                     IASTVIHGEIVIVARAGTPRPFGIVLQPGQRLPLIPPLGGVFVAWSGPDEIEPWIGKL
                     GRISEDTRKRFRRAIAAVKERGYSVGLEGDAQLLQALRGDRGQTLEEAVKGLRREEYA
                     LIEIDRKATYRLNHVGSPVFGPDGRVALGLFLIGFQGPVPGRDVERQAEQLMKASSAV
                     TAAIHGRDPR"
     gene            2807..>4037
                     /locus_tag="VNP73_01850"
     CDS             2807..>4037
                     /locus_tag="VNP73_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWL64691.1"
                     /translation="MFTRDHAIIAGLGNKGLKLATSFQKAGFRVVVIERDPNNLLIER
                     CHEAEIPVIRGDARDPIVLRRGRLPHARQLIVTCDDATNLNVILAAGAVLGGQTNSAA
                     LFVHLDDDNLGRFLKAELLEGSSLLPFRLEYFNVYDTAARTLARQAPFSRSSVGGDIG
                     PHIWIVGLDGVGESLVLHLAGQWLESRRNGDKLPLTVIGPSIEGRIEQLFLRHPELRT
                     VCDLDVKDVPLGRTLLGLIDESLKNDWGRPTLIYVCLEDEAAGLATTLALRSRDRFVD
                     VATTLVLEDDDGGIGDIVRAGQSLQGVETFGFLSDALEPWALLLGANEVIAQARHQVY
                     VDRERAAGVGPAENPSAVPWEELDEDLKEANRDYADGIGAMVAAVNGAVVPAPLVRAE
                     GPLVRFTDEEIERLARME"
BASE COUNT          717 a         1187 c         1373 g          760 t
ORIGIN      
        1 tggaccggtg acgctcggaa gcgagaggac gtcgttgctg cggtcgaggc ggctcggaag
       61 gaactcggtc gtatcgacgg actcgtcgac atcgtcggga tggcgaagta tcgcgaggcg
      121 ctggagacct cggatgagga ctgggactgg tcgttcgaca tggtccttcg ccacgcgttc
      181 ctgttctcac agcttgcggg caaggtcatg gccgacgacg gcgggggcgt catggtgttc
      241 gttgcgtcgg tgagcggtct caccggtgct ccgcggcacg cggcgtacgg agcggcgaag
      301 gccgggctca tctcgtgggt ccggtcggta gcggtcgagc tcggcccgtc gcagatcagg
      361 gtcaatgcgg ttgctccagg cgtggtccta acgccccgag tggcggcgta cctcgatgag
      421 cggagccggg agatgcaggc gaacaacgcg ccgctcaggc gggtggccgt gccggaagac
      481 atcgccgcgg cgatcctctt cctcgcctcc gacctggcgt cttacatctc gggccagact
      541 ctggtggtcg acggaggtgt gtcggcgaag ttcccgtacc cgatggagct ctgatcgtgg
      601 atcccgcttc gttcctgcgc ggcgtcgcgt ttcccggcaa cgagcgcgtg ccgtatccgc
      661 gcgccaagcc cgaggacgcc gcgcgtcttc cgctcgacac gtggtcctgc gcccgcatcc
      721 cggtgggggt tcgtctagaa ctcatcgggg acgcgtccga gatcgagatc gcttatcgca
      781 ccgagaccga agagctcggc taccgcggcg acggcgccgg aagaacgttc gccgtttggc
      841 gcgacggtcg gccggtggac tgtgaaaagg catccctggg ggaggggacg gttcggctgt
      901 cgctcgggga gactgtggac gcccgcgcga tcgtctacgt cccggaaggc atgaagccga
      961 ccatcctcga cgtcagttcg gttgggggaa cgatcgagcc ggcaccctcg cagccgaggt
     1021 gggtcgcgta cggcgactcc gtgctggagg gatggacggc ctccgagcct gcacttgggt
     1081 gggggcacca ggccgctcga gactttgggc tggacatggt gaacatgggg tacgcgggat
     1141 cagcgcgcgg ggagatcgcc tcggccgagc atgtggctga gctcgacgcc gatgtgatct
     1201 cgatcacgca cgggaccaac tgctgggcca ggactccgca cagcgtgggc ctgtaccgcg
     1261 aggggctccg ggcattcctg gacatcgtcc gaggcgggca tcccaacacc ccgatcgtgg
     1321 tggcaagccc ggtgatccgt cccgatgccg agaccacgcc gaacctgctc ggtgccagtc
     1381 tcggcgatct ccgccggatg atggaggagg tggtgcagga ccggatcgcg cacggcgatg
     1441 ccgatctcac gttggtacaa ggaggagaac tcatagatga gggccagctc gccgacggga
     1501 tccacccgaa cgacgagggg cacgcggcca tggccgccgc cctgggtccg gcgatccgat
     1561 ccgctctcga ccgaggagtt agctgatgcc gcgccgagct ccggccgtgg agcggtcgat
     1621 cgcggtcttg aacctgctcg cccagcatcc gggggagagg tacacgcttt ccgagatagc
     1681 gcgcgaccta agcctcaaca aagccacgct gcacgccatc ctgtgggccc ttagcgaagc
     1741 cggttacctg gtgcgcgacc cggaggagaa gagctacgcg ctcgggccgg ctttggtggc
     1801 agtgggcaac gccgcgctgt cgagcttctc cgcggtcgag gtcgcgcgtc cacacatgga
     1861 cgccctggcc gaagaactcg acctcgacgt catcgcgagc acggttatcc acggtgagat
     1921 cgtcatcgtg gcccgcgccg gcactccccg gccgttcggg atcgtcctcc agccgggtca
     1981 gcgccttccc ctgatccctc cgctgggcgg ggtgttcgtc gcctggtccg gaccggacga
     2041 gatcgagccg tggatcggca agctgggccg gatctccgag gacacgcgca agcggttccg
     2101 gagggccatc gcagcggtga aggagcgtgg ctactcggta gggctggaag gagatgcaca
     2161 gctgcttcaa gccctgcgcg gcgaccgagg tcagacactg gaggaagcgg tcaagggcct
     2221 acggcgcgag gagtacgcgc tgatcgagat cgatcgcaag gccacctacc ggctgaacca
     2281 cgttggttcg ccggtgttcg gccccgacgg ccgggtcgca ttaggtctgt ttcttatcgg
     2341 cttccagggt cccgtgcccg gccgggacgt ggagcgtcag gccgaacaac tgatgaaggc
     2401 ttcgagcgcg gtcaccgctg cgatccacgg ccgcgacccg cgttaggtcg gagtcaaaat
     2461 gaccgaatct caacaagcca tagcacctaa atccgaaaac cgacaggtgc gatggaccgt
     2521 caaggctatc tggcgcgata tcgagtggtg ggtagttgga tttgtgttcc tctttgcgat
     2581 tgttctcggg acccgcgggt ttctgctttc ggatgacagc tactcagtag cagatgcgtt
     2641 gtaccgcgca gtgcagctat ttgctctgaa tagcggagat gtcgacccgc cggtacctgt
     2701 acttctccag gcagctcggg tactagctcc tctcacggct agttatgccg ctatcagtgc
     2761 cctgggcgcg ctctggcgcg atcagtggca agtagccaaa gtgcgcatgt ttacgcgcga
     2821 ccacgcgatc atcgcggggc tggggaataa gggcctcaag ttggcgacat cctttcaaaa
     2881 ggcaggattt cgtgttgttg tcatcgagcg cgaccccaat aaccttctaa tcgagcgttg
     2941 ccatgaggcc gagatcccag ttatcagagg tgatgcacgg gaccccatcg ttctgagaag
     3001 gggacgactt cctcatgcgc gtcagctcat cgtcacatgt gacgacgcta cgaacctaaa
     3061 cgtgatactc gctgcaggag cagtacttgg aggtcaaacc aattcggctg cgctgttcgt
     3121 tcatctggac gatgacaacc ttggtcgatt cctgaaggct gagctactcg aaggttcatc
     3181 gctgttgccg tttcgattgg agtacttcaa tgtctatgac acggccgcca ggaccttggc
     3241 tcgacaagcg ccgttctcca ggtcgagtgt tgggggtgac ataggccctc acatctggat
     3301 cgttggacta gacggtgtcg gcgagagctt ggtcctccac ctggcagggc agtggttgga
     3361 gtcgaggagg aacggcgaca agctcccttt gacggtcatc ggtccgtcta tagaaggacg
     3421 cattgagcaa ctgtttctgc gtcaccccga gctccggacc gtttgtgacc tcgacgtgaa
     3481 ggacgtcccc ctaggacgaa cacttcttgg tctgatcgac gagagtttga aaaatgattg
     3541 gggacgtccg acgctgattt acgtttgtct agaggatgag gcagcgggat tggcgacaac
     3601 actcgcgctc cgaagtcgcg atcgattcgt cgatgtcgct acaaccttgg tccttgagga
     3661 tgacgatgga ggcatcgggg acatcgtccg agccgggcag agccttcaag gggtcgagac
     3721 tttcgggttc ttgagtgatg ccttggagcc ctgggccctg ctcttggggg caaacgaggt
     3781 cattgcccaa gccagacatc aggtctatgt cgaccgtgaa agggccgcgg gtgtcggacc
     3841 agccgagaat ccctcggcgg ttccgtggga agagctcgac gaagatctaa aggaagcaaa
     3901 tcgcgattac gccgacggca tcggagcgat ggttgccgcc gtcaacgggg cagtcgtgcc
     3961 tgcgcccctg gtccgagctg agggtccgct ggttcgcttc acggatgagg aaatcgagcg
     4021 tttggcgcgg atggaac
//
LOCUS       DATMED010000073         6850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_56894, whole genome shotgun sequence.
ACCESSION   DATMED010000073 DATMED010000000
VERSION     DATMED010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56894"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VNP73_01855"
     CDS             <1..903
                     /locus_tag="VNP73_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWL64692.1"
                     /translation="RTLSETGVAAPLIALMYAFTIIFREGLEAVLVVAAVLGYLEASR
                     NTQYRGAVLQGVAGGVGATVLLFVAVALFVRLAPVQRELLEAGTALLAVGVLFYVSFW
                     LISRLEQRRWMEFVKAKVWSAATTGSTLALAGVGFTAVFREGFETVLFYQALISFAEG
                     LLSWVVAGSVAGVVVLGVIGWIIFKAGRKIPMKAFLTTAVSLVMVLSVAFACNAVRGF
                     QEAALLPVTHLESLPDLPIFLSQLTGWYPTRETIIAQAALAIVYALGAVWTFVILPRR
                     ERRHGPVREPQVVTAEREPERTSV"
     gene            903..3008
                     /locus_tag="VNP73_01860"
     CDS             903..3008
                     /locus_tag="VNP73_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWL64693.1"
                     /translation="MSVRIGVDVGGTFTKAVACAAETGEVLARSIVPTSHQAPAGVAD
                     GAIQALGEVAEDVAARELGPIQLVAHSTTQAVNALLEGDTSVVGVLGIGRRPDVKRAR
                     RRTAVGQVRLAPGRKLVTRHSFIDASGGLERAQIVAAIQELVDEGAEVLCISEAFGVE
                     DARSEWLALEVAEDLGVPACAGHELTGLYGLEMRTVTGALNASILPTALRTARMVEEA
                     VARDIPGVPLLVMRGDGGAVGIQAMKRHPLLTAFSGPAASVAGALHHVEVHGGVVVEV
                     GGTSSNISTIKGGRPILSYIRVLDHVTCVRSVDVRVVGVAGGSLLRIARRMGRPRLID
                     VGPRSAHIAGLPYACFSTRAELTGATPALIAPRPGDPAEYLVLETRAGRRVAITLTCA
                     ANALGEVTAGSYAEGNADAAMCALEIVANWLGTDVRSLAGGALKAAATKIGGAVKEAA
                     AEQSLVEPQVIGLGGGAGALMSALGHVTGLPWSIPREAEVISSVGDALSLVRVEVERT
                     LPRATAEAVAAVHSAAEEAALGAGAAPHTIQLESEAVPERGALRVVAYGSASLEAGDG
                     AIPLNGGRKDASARSALGEAAREVATNPFYSVYVDGTGGDRAFAVIDRVGAVVAEGSG
                     TVLTGVGAEVAAAIEERVPALVRHFGPIAVAPALRIIRGARVIDLTLISDPDAAVEAA
                     LAECSLAHEEPVVALLSRA"
     gene            3010..3447
                     /locus_tag="VNP73_01865"
     CDS             3010..3447
                     /locus_tag="VNP73_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64694.1"
                     /translation="MAIYRPPKARWPLALGTGIASLLIGAAIGLAIGTQDPDPVESAQ
                     ALRSTLVAAAGSIEVAAIEYEEAVVDGTVEREAEYEGSLAALDSSNARYREVRSALEL
                     LAPEAADEIDSLFGRCRSLMEETADAERVTECLADLELVLTGS"
     gene            complement(3427..5538)
                     /locus_tag="VNP73_01870"
     CDS             complement(3427..5538)
                     /locus_tag="VNP73_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64695.1"
                     /translation="MRTRLGRASLIVGLLACVLSASLAFGAPVSSTGAPTAAPTKSPA
                     PKGRCKDGKTNPNCPTPTPTPTTTTSPTPTPTPTIPASAGKVGFGTRKITPVGNTAEW
                     PPAEWKQFFTPHPETEVWGEPYTDSNGNDCYDGPHFTDVPAPDDPNIEGQPEPHVDQP
                     WNSAGDAHENGAFSVNGVTIYGDPQSSGKWDGVWANAGFGSKCTLGAKDDTWARAVVV
                     EVDGKTVAMVSLDVVGLFNIEVKRAREELAVRYPDIQIDELVVSSTHTHEGVDTMGYW
                     GQTLGIDGKFPAYNAFIRSQLIDAVADAYEAREPAVAKFAQSEFTLGIRDSRPPHRID
                     PYLLSAQFIRPSDGSTIGSIVNWSNHPEAQGSNNQLVSSDFPHGTRQALERDLGGTAV
                     YFSGSVGGLMTPLGVDIPGFGSSVSWERTYRLGELVAEAAEGALASAPLEGINDLAAK
                     RREFYMQADNTALNGLNGAGVFDIPTYVGANSWGLDGHTEGIYVGRTGPQFKTEMVAV
                     RFGPALFLTVPGELFPEIELGGYGRPDCAAADTGRPYEPVISEQFDAEYQFILGLGQD
                     ELGYIVPGYDFWLRHVDDGPDGSDGNYEGILALGALEEDDPCGEGHYEETVSGSSVMA
                     PWVACVAAELAGKDPWALSSTDDGYAACTQENTHMNPYGVNPDALGEPPVGTQGHTHG
                     YEHPHDYYEGLTARELRAGQH"
     gene            5625..5969
                     /locus_tag="VNP73_01875"
     CDS             5625..5969
                     /locus_tag="VNP73_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64696.1"
                     /translation="MTKGTIVLAAVMLLAPSAAAGARAARSDEGCEAQNPLQPSCSFT
                     VTHTSSTPVTGAGGIGDWIVIVKRGDETLKFESLPDGRPTMREISFKIGDKVTAKALS
                     PGSGLTAGHFDS"
     gene            5966..6580
                     /locus_tag="VNP73_01880"
     CDS             5966..6580
                     /locus_tag="VNP73_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64697.1"
                     /translation="MRSKALACASALLLLAAPTFALAQEHDHHAGGECHPTPEQKHEA
                     AEFARRTMAAAKPYEDPAYAFENDFIPWVDAWKPVFHFVKYEHYYDWKVLDPNRPEAF
                     VYAKTNTGLQLIGVMFSMEDPGRKPPDFGGCITRWHTHPQCVAPIGYSHIWEEDWGGC
                     PPGWEEDNGGSELMLHVWTVPMEGGPYAYHPDSSWECWPQPAPC"
     gene            6610..>6850
                     /locus_tag="VNP73_01885"
     CDS             6610..>6850
                     /locus_tag="VNP73_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWL64698.1"
                     /translation="MEVGHVLVDILVVLVVAKCAAEISLRIGAPTVAGELVAGILIGP
                     SLLGWVEESAALAILAELGVLLLLVEVGMQMEIGDL"
BASE COUNT         1094 a         2110 c         2348 g         1298 t
ORIGIN      
        1 cggactcttt cggagacggg ggtggctgct cctcttatcg ccctcatgta tgcgttcacg
       61 atcatcttcc gtgaggggct tgaagcggtg ctcgtggtcg ctgcagttct cggatacctc
      121 gaagccagcc gcaacacgca atacagaggg gccgttttgc agggcgttgc cggaggtgtc
      181 ggcgcaaccg tcctcttgtt cgtcgccgta gcactattcg ttcgcctggc cccggttcaa
      241 cgagaactat tggaggcagg cacggccctc cttgcggtcg gcgtcctctt ctacgtgtcc
      301 ttctggttga tctcgcgttt ggagcagcgt cgctggatgg agttcgtgaa agcgaaggtg
      361 tggtccgcgg ccaccaccgg ttcgacgttg gctctggcag gtgtcggatt cacggcggtc
      421 ttccgggaag gattcgagac cgttctcttc taccaggcac tgatctcgtt cgccgagggg
      481 ttgctgtcgt gggtggtggc cggttcagtc gcgggtgtcg tggtactcgg cgtcatcggg
      541 tggatcatct tcaaggccgg tcgcaagatc ccgatgaagg cgttcctgac tacggcggtc
      601 tcgttggtga tggttctatc ggtcgcattc gcctgtaacg cggtgcgcgg cttccaggag
      661 gccgcgctac ttcccgtaac ccatctcgag tcgttaccgg accttccgat cttcctgtct
      721 cagctcaccg gctggtatcc aacgcgcgag acgatcatcg cccaagcggc gctcgccatc
      781 gtttatgcgc tgggcgcggt ctggacattc gtcattcttc cccgtcgcga gcggaggcac
      841 gggcccgtcc gcgagcctca ggtcgtgacg gccgagcgcg agcccgagcg cacttcggtc
      901 tgatgtcggt acgcattggg gtcgacgtgg gcgggacgtt cacgaaggct gttgcgtgcg
      961 cggcagagac cggcgaggtc ctggctcgct cgatcgttcc gacctctcac caggctccgg
     1021 cgggcgttgc cgacggagcg atccaagcct tgggcgaggt cgcggaagat gtcgccgcgc
     1081 gggaactcgg tccgatccag ctcgtcgcac actcgacgac ccaagcggtc aacgcgctgc
     1141 tcgagggtga cacctcggtc gtaggcgttc tcggcatcgg tcgacgcccg gatgtcaaaa
     1201 gggcccgccg ccgcacggcg gtcggtcagg tgcgcctggc gccgggtcgg aaactggtca
     1261 cgcgacactc gttcatcgat gcatccggcg gccttgagcg ggcccagatc gtcgcggcga
     1321 tccaagagct cgtcgacgag ggagctgagg tcttgtgcat ctcggaagcc tttggcgtcg
     1381 aagacgcacg cagcgagtgg ctcgcgctcg aagtggcgga ggatctcgga gtgcccgcct
     1441 gcgccggtca cgagctgacc ggtctttacg ggctcgagat gcggacggtc acgggcgcgc
     1501 tgaacgcaag catccttcca acggcactgc gaacggctcg gatggtggaa gaagccgtgg
     1561 ctcgagacat tcccggagtg ccgctgttgg tcatgcgtgg agatggtgga gccgtcggta
     1621 tccaagcgat gaaacgtcac cccctcctta cggctttcag tgggccggcc gcgtcggttg
     1681 ccggtgcgtt gcaccacgtg gaggtgcatg ggggagtcgt cgttgaggtc ggcggcacct
     1741 ccagcaacat ctcgacgatc aagggtggac gtccgatcct gtcttacatc cgggtgctgg
     1801 accatgtgac gtgcgttcgc agcgtggacg ttcgcgtcgt gggcgtcgcc ggtgggagct
     1861 tgttgaggat cgctcggagg atgggacgcc cccggctgat cgacgtcggc ccacgctccg
     1921 cgcacatcgc cggtcttccg tacgcatgct tctccacgcg tgctgagttg accggggcga
     1981 cgccggcgct gatcgcgccc cggcccggtg acccggccga gtacctggtg ctcgaaacaa
     2041 gggccggacg ccgcgttgcc atcactttga cctgtgcagc caacgctctg ggcgaggtaa
     2101 ccgcgggctc gtatgcagaa ggcaacgcgg atgcggcgat gtgcgcgctc gagatcgtcg
     2161 ccaactggct gggcacggac gtccgttccc tcgccggcgg agcgctgaag gcagccgcca
     2221 ccaagatcgg aggcgccgtt aaggaggctg ctgccgagca atctctcgtc gaaccgcaag
     2281 tgatcggact cggtggaggc gccggtgcac tcatgtccgc cctaggtcat gtaaccggtt
     2341 tgccgtggag catcccgcgc gaggccgaag tgatctcatc ggtcggcgac gctctgtcct
     2401 tggtgcgcgt cgaggtcgaa cggacgcttc cacgtgcgac cgccgaggcg gtggccgccg
     2461 ttcactcggc cgccgaagag gccgcactag gagccggagc cgcgcctcac acgatccagc
     2521 ttgagagtga ggccgttccc gagcggggtg cattgagggt ggtggcttac ggctcggctt
     2581 cactcgaagc cggcgacggg gctatcccgc tcaacggcgg caggaaggac gctagcgccc
     2641 ggtcggcgtt gggcgaggcc gcacgcgagg tcgcgacgaa tccgttctat tccgtctatg
     2701 tcgacggaac cggtggagac agggcgttcg ccgtgatcga ccgtgtgggc gccgtcgtgg
     2761 ccgagggttc cggaacggtc ctgaccggag tgggggccga ggttgcagcg gcgatcgagg
     2821 aacgcgtgcc cgctctcgtg cgacatttcg gtcccatcgc ggtggcgccg gcgcttcgga
     2881 taatccgggg agcgcgagtc atcgatctaa ctttgatctc ggatcctgat gccgcggtcg
     2941 aggctgcact ggccgaatgt tccttggctc acgaggagcc ggtcgtagcc ctgttgtcgc
     3001 gcgcctgacg tggcgatcta ccgcccgccc aaggcgcgct ggccgctcgc gctcgggacc
     3061 ggcatcgcga gtctcttgat cggtgcggcg atcggtcttg cgatcggtac ccaggacccc
     3121 gatccggtcg agtccgctca agccctcaga tcgacccttg tcgcagccgc cggttcgatc
     3181 gaagtggccg cgatcgaata cgaagaagcg gtggtcgacg gcaccgtcga gcgggaggcc
     3241 gaatatgagg ggtcgctcgc ggctctcgac agcagcaacg ctcgttaccg ggaggttcgc
     3301 agtgccctgg aactacttgc ccccgaggcc gccgacgaga tcgactcttt gttcgggcgt
     3361 tgccggagcc tcatggagga gacggcggat gccgagaggg tgacggagtg cttggccgat
     3421 ctcgagctag tgctgaccgg ctcttagctc tctggcggta aggccttcgt aatagtcgtg
     3481 gggatgctca tacccgtgcg tgtggccctg agttcccacc ggtggctcgc cgagcgcgtc
     3541 gggattgacc ccgtaggggt tcatgtgggt gttctcctgc gtgcaagcag cgtagccatc
     3601 atcggttgac gagagagccc acgggtcctt gcccgccaac tcggccgcga cgcacgccac
     3661 ccacggagcc atgaccgagg agcccgacac ggtctcctcg tagtgacctt cgccgcaggg
     3721 gtcgtcctcc tcgagagcgc ccagcgccag gatcccttcg tagttcccgt cactgccgtc
     3781 cggaccgtcg tcaacgtgac gcaaccagaa gtcgtagccg gggacgatgt agccgagctc
     3841 gtcctggccg aggccgagga tgaactggta ctcggcatcg aattgctcgc tgatcacggg
     3901 ctcgtacggc cgcccggtgt cggcggcggc gcagtccggt cgtccgtagc cgccgagttc
     3961 gatctcgggg aagagctcgc ccggtaccgt gaggaagagg gcgggaccga atcgaacggc
     4021 caccatctca gtcttgaatt gaggaccggt gcggccgacg tagatgccct ccgtgtgccc
     4081 gtccaggccc catgagttcg caccaacgta cgtcgggatg tcgaagacac cggcaccgtt
     4141 caaaccgttc agggctgtgt tgtcggcctg catgtagaac tcccggcgct tcgcggcgag
     4201 atcgttgatc ccttcgagcg gcgccgacgc gagggcgccc tcggccgctt cggccactag
     4261 ttctccgagc cggtaggtgc gttcccacga aacgctgctg ccaaagcccg ggatgtcgac
     4321 cccgagcggt gtcatcaatc cgccgaccga acctgagaag tacacggccg tgccgccgag
     4381 gtcacgctcc aatgcttggc gcgtgccgtg agggaagtcg gaggagacga gctggttgtt
     4441 cgagccctgt gcctcggggt ggttcgacca gttgacgatc gagccgatcg tcgagccgtc
     4501 ggagggcctg atgaattgtg ccgagagcag gtaggggtcg atgcgatgcg ggggccgcga
     4561 gtcgcggatc ccgagggtga actcggactg cgcgaacttc gccacggccg gctctctcgc
     4621 ttcataagcg tcggccactg cgtcgatcaa ctgtgaccgg atgaacgcgt tgtaggcggg
     4681 gaacttgccg tcgataccga gtgtctgtcc ccagtagccc atcgtgtcga cgccctcgtg
     4741 tgtgtgcgtg gacgagacga ctaattcgtc gatctggatg tcgggatagc ggaccgcgag
     4801 ttcctcgcgt gcccgcttca cctcgatgtt gaacagtccg acgacgtcga gggagaccat
     4861 cgctaccgtc ttgccgtcga cctcgaccac aaccgcacgg gcccaggtgt cgtccttcgc
     4921 ccccagcgtg cacttggaac cgaaacctgc gttcgcccag acgccgtccc acttgcccga
     4981 gctctgcgga tctccgtaaa tcgtcacgcc gttcacgctg aaagcgccgt tctcgtgcgc
     5041 gtctccggcg ctgttccagg gttggtctac gtgcggctcg ggctgtcctt cgatgttggg
     5101 gtcatccggt gcgggcacgt ccgtgaaatg ggggccgtcg tagcagtcgt tcccgttcga
     5161 gtccgtgtag ggctcacccc agacctcggt ctcgggatgc ggggtgaaga actgcttcca
     5221 ctcggcaggg ggccactcgg ccgtgttgcc gaccggcgtg atcttgcgcg tgccgaagcc
     5281 gaccttgccg gccgacgcag ggatcgtggg tgttggcgtt ggagtgggag aggtggtcgt
     5341 cgtgggggtc ggggtggggg tggggcagtt cgggtttgtc ttgccgtcct tgcagcgccc
     5401 tttgggagcg ggggacttcg tgggagcggc tgtgggagca ccggtgctgc tcacgggggc
     5461 tccgaacgcg agcgaggccg acaggacgca ggccagcagg ccaacgatca gggacgctcg
     5521 acccagacga gttctcacgg aggcataggt tccgccctac cggaggatct cctgcttgac
     5581 ctgctcgggc aggaccgctt cggtgggtag agaagtgtca ttacatgaca aaagggacga
     5641 tcgttctggc tgccgtgatg ctgctcgccc cttcggcggc agccggcgcg cgcgcggctc
     5701 ggagtgacga gggatgcgaa gcccagaatc cactgcagcc gagctgctcc ttcaccgtca
     5761 cccacacgtc gagcacgccc gtgacggggg ccggtgggat cggcgactgg atcgtcatcg
     5821 tgaagagggg agacgagacg ttgaagttcg agagtcttcc cgatggccgg cccacgatgc
     5881 gggagatctc tttcaagatc ggcgacaagg tgaccgcgaa ggccttgagt cccggcagcg
     5941 gcctgaccgc agggcatttc gactcatgag atccaaagct cttgcatgcg catccgcact
     6001 gttgctgctc gcggccccca cgttcgcgct cgctcaagag cacgatcatc acgctggggg
     6061 agagtgtcat cccacaccgg agcagaagca tgaggcggcc gagttcgctc gccgaacgat
     6121 ggctgcggcc aagccctacg aggatcctgc gtacgcgttc gagaacgact tcatcccatg
     6181 ggtggatgca tggaagccgg tcttccattt cgtgaagtac gagcactact acgactggaa
     6241 ggttctagac ccgaaccgac cggaggcctt cgtatatgcg aagacgaaca cgggactaca
     6301 actgatcggc gtcatgttct cgatggagga tcccggtcgc aagccgccgg acttcggtgg
     6361 atgcatcacg cgctggcaca cacacccaca gtgtgtcgct ccgatcggct acagccacat
     6421 ctgggaagag gattggggtg gctgcccgcc gggatgggaa gaggacaacg gcggatccga
     6481 gttgatgctc cacgtatgga cggtccccat ggaaggcggg ccgtacgcct atcaccccga
     6541 ctcaagctgg gagtgctggc ctcagccggc cccctgctga tctcgccacc tcacccttag
     6601 attcacccga tggaagtcgg gcacgtcctc gtagacatcc tggtcgtcct cgttgtggcg
     6661 aagtgtgccg cggagatctc cttgcggata ggcgcaccga ccgtcgccgg agagctcgtc
     6721 gcagggatcc tcatcggacc atcgctcctc ggatgggtcg aggagagcgc cgctctcgcg
     6781 atcctggcgg agcttggcgt tctgttgctg ctcgtggaag tcgggatgca gatggagatc
     6841 ggcgatcttg
//
LOCUS       DATMED010000074         2726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_435375, whole genome shotgun sequence.
ACCESSION   DATMED010000074 DATMED010000000
VERSION     DATMED010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435375"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VNP73_01890"
     CDS             <1..250
                     /locus_tag="VNP73_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64699.1"
                     /translation="VLVRIDADTNADRRPDVWVTYANGNPALQYEDTDFDGAPDQAFD
                     LATEEPIALEGQGGNLPVDPFEQISCGGFSEYWKTWRN"
     gene            422..1792
                     /locus_tag="VNP73_01895"
     CDS             422..1792
                     /locus_tag="VNP73_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64700.1"
                     /translation="MDEPIAESGIPSEESAQDDSDLPTADGDEPAEESVFTSTADSEE
                     EDASADGSDAEEDDGVVEDLGEGIENEESADADGATQGQEAPGGGGLGQRAESAVEGV
                     IIGTVVGGQIAGGYGAAVGAAVFGLYGLITGDVPFDSGQNKSRPGAGGDADEALETEI
                     EEELEKQGALEDEIEAELKRQEALLEQIDRQEEINQEIQKERDRRVALEQADPLSAPP
                     VPYLRDIPDSIFDSEERLVEGTNKVVKSLDADRDGRPEMEKVYDNASGNLDRVSQDTN
                     YDGLMDTQFEYNPAGEIVATVEDTDQDGVPDRWITFEGGSGTRVEVDRNNDGVRDGFL
                     SYSEGVLASEEYDDDGDGVINRSVDYANKSRSVEREDRDGDGKVDFWTYYEPGGRPVR
                     VEKDTKQAGKPDVWEYYEGTDPGNMVIVRKEEDLNGDSAVDVTSYYENGRLNRKEIQN
                     PEALKK"
     gene            complement(1942..>2726)
                     /locus_tag="VNP73_01900"
     CDS             complement(1942..>2726)
                     /locus_tag="VNP73_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWL64701.1"
                     /translation="RALQAVKLRGSNGGTDMQAGIKLAVSELAGLVGSTSEPRPAARK
                     VMLLLTDGLPSLPFGQVNVQDPQDIEATVAAGNLAAEAGVLINVFGLGPAAGGNDRPQ
                     AVTEIARLTSGRYVAVRRPGDVVTLLSGISFAAVEDVVAVNLTIGQESGRQDIVLMPD
                     GSFRGFVPVAPGLNRIRVSALASDGTRGSTEFQITYRLQDLSDSELQAELERVRDRNR
                     DIEVLTERRRQDAFRRSERERALTLEVEGDTGEPESKEKKTP"
BASE COUNT          563 a          815 c          894 g          454 t
ORIGIN      
        1 cgtcctggta cgcatcgatg cagacacgaa cgcagatcgt cgccccgacg tttgggtgac
       61 ctacgcaaac ggaaaccctg ctctacagta cgaggatacg gacttcgacg gagcgcccga
      121 tcaggccttc gacctcgcca ccgaggagcc gatagcacta gaggggcaag gcggtaactt
      181 gccggtggac ccgttcgagc agatctcctg tggcgggttc agtgaatact ggaagacatg
      241 gcgtaactag aggtctagac tagccgagac cagactgggg gatcccatca tgctgcgagt
      301 cgaatccaac ccatttttcc atgggcccac ccgttgtcgc gggctcgcag cttgggcggt
      361 cgtcctggtg tcggcggttt cccttgcctg cgccagcacg gccccggtgc cgccggcccc
      421 gatggatgaa cccatcgccg agagcggcat ccccagcgaa gagtcggcgc aggacgactc
      481 ggacctgccc acagccgatg gtgacgagcc tgccgaagag agcgtcttca cctccacggc
      541 ggactccgag gaagaggacg cttcggccga tggctcggat gccgaggaag acgatggcgt
      601 cgtcgaagat ctcggagagg gaatcgagaa cgaggaatcc gcggacgcgg acggagcgac
      661 tcagggtcag gaagcacccg gtgggggcgg cctgggccag cgggcagaga gcgcggtcga
      721 gggcgtcatc atcggcaccg tggtcggtgg ccagatcgcg ggcggatacg gcgcggcggt
      781 gggcgcagcg gtcttcggcc tctacgggtt gatcacgggc gatgtccctt tcgatagcgg
      841 tcagaacaag agccggccgg gagcgggcgg cgacgcggac gaagcgctcg agaccgagat
      901 cgaggaagag ctcgagaaac agggtgcgct cgaggacgag atcgaagccg agctgaagcg
      961 ccaggaagcg ctcctcgagc agatcgaccg ccaggaagag atcaaccagg agatccagaa
     1021 agagagggat cgtcgggttg cgctcgagca ggccgatccg ctctctgctc ctccggttcc
     1081 ctaccttcgc gacattcccg acagcatctt cgactccgag gagcgcctgg tcgaagggac
     1141 caacaaagtc gtcaagtcgt tggatgcaga tcgcgacggt cgccccgaga tggagaaggt
     1201 ctacgacaac gcgtccggca atttggatcg tgtttcacag gacacgaact acgacggcct
     1261 gatggatacc cagttcgaat acaacccggc cggcgagatc gtggccaccg tggaggatac
     1321 ggaccaggac ggagttcccg accgttggat caccttcgaa ggcggtagcg gaacgcgagt
     1381 cgaggtcgat cggaacaacg acggcgttcg cgacggcttc ctgtcctata gcgaaggcgt
     1441 tctggcatcc gaggaatacg acgacgatgg cgatggagtg atcaaccgga gcgtcgacta
     1501 cgccaacaag agccgctcgg tcgagagaga ggatcgagac ggagatggca aggtcgattt
     1561 ctggacctac tacgagcccg gtggtcgtcc ggttcgtgta gagaaggaca cgaagcaggc
     1621 gggtaagccg gatgtatggg agtactacga gggcacggat cccggaaaca tggtgatcgt
     1681 tcgaaaggaa gaggatctca acggcgattc agcggtcgat gtcacctcgt actacgagaa
     1741 tggccgcctg aaccggaagg agatccagaa tcccgaggcc ttgaagaagt gacccttcga
     1801 atcacctagg tctccgagct cggctcgatg aagagggggt ctcagcaatg aggctccctt
     1861 tttcttgcgc ttgcgagggt tgccgcccga atcggtagcc accgcttgtc gggctacagg
     1921 ctcagacagc ctcttcgatt ctcacggcgt cttcttctct ttcgactccg gctccccggt
     1981 gtcgccctcg acctccaagg tcagagcgcg ttcgcgctct gaccggcgga aagcgtcctg
     2041 ccgacgacgc tccgtcaaca cctcgatgtc ccggttgcgg tcgcgaacgc gctcgagctc
     2101 cgcttggagc tccgaatccg acaagtcttg gagtcgatag gtgatctgga actcggtcga
     2161 tccgcgggtt ccatcggagg cgagcgcaga gacccggatt cgattgagcc cgggagcgac
     2221 gggcacaaag cctcgaaagg agccatcggg catcagcacg atgtcctgac gccccgactc
     2281 ttgtccgatc gtcagattca ccgccaccac gtcttcgacg gcagcaaacg agattccgga
     2341 gagcagcgtg accacgtccc cggggcggcg gaccgccaca tatctgccgc tggtcagccg
     2401 tgcgatctcg gtgaccgctt gtgggcggtc attgcctccg gcagcggggc ccaggccgaa
     2461 gacattgatc agcacccctg cttcggcagc tagattgccc gccgcgacgg tcgcttcgat
     2521 gtcctgaggg tcttgcacgt tcacttgacc aaaaggaaga gacggcaggc catcggtcaa
     2581 aagcagcatc acctttcgcg ccgcgggacg tggctcggaa gtggatccaa ccaggccagc
     2641 aagctcggat accgccagct tgattccggc ctgcatgtcg gtgccgccgt tcgatccgcg
     2701 cagcttgacc gcttgaagag cccgcc
//
LOCUS       DATMED010000075         4769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_437259, whole genome shotgun sequence.
ACCESSION   DATMED010000075 DATMED010000000
VERSION     DATMED010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437259"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="VNP73_01905"
     CDS             complement(<1..1902)
                     /locus_tag="VNP73_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWL64702.1"
                     /translation="MLVVNGLAKSFGARPLFRDAELKVFARDRVALVGPNGSGKTTLF
                     DMIAAEQQPDAGSITLTKDVVVGYLRQETDALRGRSLLEEVTSLDTLSDAAHRLAVLE
                     TEMAELEPGPERDRVVAEYGRLHDRFEAGGGYSVEHEAKRILGGLGFDTSDHARPTET
                     FSGGWLMRIALAKLLLAGPDLLMLDEPTNHLDLESVVWLERFLRDYEGAVLLISHDRE
                     LINTLASKVVELRDYRLWSYTGDFASFVEQRELEIAQAEAAAKNQARKIAQTERFIER
                     FRYKNTLASRVQSRIKALEKMERIEVPRSRRKQMHLRWPTPPRSGRVVVEMESLRFGY
                     DPDRPVYEDLELVLERGEKTALVGPNGAGKSTLLKLIAGVLEPQGGGRTLGHKVELAY
                     FAQHQIEALQPANTVLQELRTAMPAGLDVDARKLLGRFLFTGDDSDKKVAVLSGGERT
                     RLALAKLLVAPVNLLCLDEPTNHLDMQSRDVLEEALQEYEGSIVLITHDRHLIRSVSN
                     RIVEVVDGAVTSFNGGYEYYLDKREPREEEPERGAAPEKRTDRSAAGPKTKEQKRVEA
                     EARAQTKKLRDTIARIEKELDDVGAEIARLEETFAAPDVYASGTDVVDLTKRYESAKK
                     RIARLEQAWA"
     gene            1926..4044
                     /gene="glgX"
                     /locus_tag="VNP73_01910"
                     /pseudo
     CDS             1926..4044
                     /gene="glgX"
                     /locus_tag="VNP73_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020120084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
     gene            4034..>4769
                     /locus_tag="VNP73_01915"
     CDS             4034..>4769
                     /locus_tag="VNP73_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWL64703.1"
                     /translation="MPSKHLRATYRVQVHKDFTLDRVAGLTDYFAELGFSHVYCSPYL
                     SSRPGSLHGYDVVNHSTVDDELGGAAALERLMAALEAKQMGHILDVVPNHMTIAERAN
                     EWWWDVLRNGPYSEYATFFDVDWDPPEARLRRKILIPILGDHYGRVLEKGEIKVEEEA
                     DEFVVRYYDHVLPVAPDSISYAFAEEGAHDIRRINDDAALLHSLLELQHYRLAYWKTA
                     GKELNYRRFFAINDLAALRVDRDDVFD"
BASE COUNT          877 a         1593 c         1421 g          878 t
ORIGIN      
        1 ggcccacgct tgttcgagtc tcgcgatcct tttcttagcc gactcgtagc gcttcgtcag
       61 atcgaccacg tccgtacccg aggcatacac gtcgggggcc gcgaacgttt cctcgaggcg
      121 cgctatctcg gcccccacgt cgtccaactc cttctctatg cgagcgatcg tgtcgcgcaa
      181 cttcttcgtc tgggcacgag cttcggcctc aacccgcttc tgctctttcg tcttggggcc
      241 cgccgcgctg cgatcggttc gcttctcggg ggccgctccg cgctcgggct cctcttcacg
      301 cggctcccgc ttgtcgaggt agtactcgta gcccccgttg aaggaggtca ccgctccatc
      361 caccacctcg actattcgat tcgacaccga gcggatcagg tgccggtcgt gggtgatcaa
      421 cacgatgctt ccctcgtatt cctgaagcgc ttcctccagc acgtcacgac tctgcatatc
      481 gaggtggttg gtcggctcgt cgaggcacaa gagattcacc ggcgcgacga gcagcttggc
      541 cagcgcgagc ctggtccgct ccccaccgga cagcaccgcc actttcttgt cggaatcgtc
      601 gccggtgaac aggaaccgtc ccagaagctt tcgtgcatcc acgtcgagac cggccggcat
      661 cgcagtccgc agttcctgaa ggaccgtatt cgccggttgc agcgcctcga tctgatgctg
      721 cgcgaaatac gccagctcga ccttgtggcc cagcgtccgg cccccaccct gcggctcgag
      781 cacaccggcg atcaacttga gaagcgtcga cttccccgcc ccgttcggtc cgacgagcgc
      841 ggtcttctcg cctcgctcca gcacgagttc caggtcctcg tagacggggc gatcggggtc
      901 gtatccgaac cgcaacgact ccatctccac gacgacacgg cccgaccgcg gcggggtcgg
      961 ccatctgagg tgcatctgct tgcgtctgct cctcggcacc tcgatccgct ccatcttctc
     1021 gagtgccttg atacgactct gcacgcggct tgcgagggtg ttcttgtacc ggaagcgctc
     1081 gatgaatcgt tcggtctgag cgatcttgcg cgcttgattc ttggccgccg cttccgcctg
     1141 cgcgatctcc agctcgcgct gctccacgaa actggcgaag tcaccggtat aagaccaaag
     1201 gcggtaatcc cgaagctcga cgaccttgga ggccagcgtg ttgatcagct cccggtcgtg
     1261 cgagatcaag agcacggccc cctcgtagtc gcgcaggaag cgctccagcc acacgaccga
     1321 ttcgagatcc aggtggttcg tcggctcgtc gagcatcaac aggtcgggac cggcaagcaa
     1381 cagcttggcc agcgctatcc gcatcagcca cccgcccgag aacgtctccg tcggccgggc
     1441 gtgatccgag gtatcgaatc cgagacctcc caagatgcgc ttggcctcgt gctcgaccga
     1501 atagccgccg cctgcttcga agcgatcgtg caacctcccg tactcggcaa cgacccggtc
     1561 ccgttcgggc cccggctcga gctccgccat ctcggtctcg agcacggcga ggcgatgcgc
     1621 tgcatccgac agggtgtcga gcgaggtgac ctcctcgagc aacgagcgcc ctctgagcgc
     1681 gtccgtctcc tgccggagat aaccgacgac cacgtctttc gtcagcgtga tcgatcccgc
     1741 gtcgggttgc tgctccgcgg cgatcatgtc gaacaaggtc gtcttgcccg aaccgttcgg
     1801 tcccacgagc gccacgcgat cgcgtgcgaa gactttcagc tcggcatccc ggaacaaggg
     1861 ccgagccccg aacgacttgg cgagaccgtt aacgaccagc atcccccaag cgtagggttc
     1921 cgcccatggg ggtttggccg ggtagcccgt tcccgctcgg cgccagctgg gacggaacag
     1981 gcacgaattt cgcagtgttc tctgaaggcg cagagcgggt cgaactgtgc ctcttcgacg
     2041 attccggcac cgagtcacgc ctggatctga ccgaggtcac gacccacgtc tggcacggtt
     2101 tcgtcgaagg agtcgggccc ggccggcgct acggctaccg gatgcatggg cccttcaagc
     2161 ccgagcacgg cctgcggttc aatccgaaca agctgctgat agacccctat gcactggcga
     2221 tcgagggcga cgtcgagtgg ggtgaggagg tcttcggata ctccttcgtc gatcccggca
     2281 aggacctctc cttcgacgat cgcgacgacg cggcccgggt ccctaagtcg atcgtcgtcg
     2341 acggatcgtt ctattgggaa gacgacaaga agccgaacat cccgtggcat gaaacggtcc
     2401 tttacgagac ccacgtcaag ggcttcacga tcaacaacct gggactggag ccggccatcc
     2461 gcggctcgta tgccgggatc ggccatcccg caacgatcga acacctcctg cttctcgggg
     2521 ttaccgcgat cgagctgctg ccggtgcacc acctgatcca cccgcagcat ctcgtggaca
     2581 aaggcttacg caactactgg ggctacgact cgatcggtta cttcgcgccg tattccggct
     2641 acagctcggc cggtgggcgc gggggccagg tgaacgagtt caaagagatg gttaaggcac
     2701 ttcacgtcgc gggcatcgaa gtgatcctcg acgtcgtcta caaccacacc gcggagggca
     2761 atcatctcgg ccccacgctg tcgttgagag gactcgacaa cacttcttat taccggctcg
     2821 tcgaagaaga ccctcgctac tacttcgata cgacgggcac cgggaactca ctcaacgttc
     2881 gtcacccgca atcgctgaag ctgatcaccg attcgttgcg ctactgggcc gaggagatgc
     2941 acgtcgacgg cttccgcttc gaccttgcgg ccacccttgc ccgccagttc tacgaagtcg
     3001 accggctgag cgcgttcttc gacatcatcc accaggaccc cgtcctgtcc cgcatgaagc
     3061 tgatcgccga gccatgggac gtcggtgaag gcggctatca ggtgggtaac ttccctcccg
     3121 ggtggtcgga gtggaacggt aagtaccggg actgcgtgcg ggatctatgg cgcggcgagc
     3181 cggcgacact gggcgagttc gcgtttcgct tcacgggatc gtccgacctc taccaggacg
     3241 acggcagaca cccaacggcc agcatcaatt tcatcaccgc gcacgacggc ttcacgctgc
     3301 acgacctcgt cacctataac gagaaacaca acgaggcgaa cggcgaaggc aacgccgacg
     3361 gcgagagtca caaccgctcg tggaaccacg gcgccgaagg agagaccgac gatcccgcca
     3421 tccgagcact gagggaacgt cagaagcgga acttcctcac cacgctggcg ctcagccagg
     3481 gggtcccgat gttcctcggc ggcgacgaga tgggccgaac gcaggagggc aacaacaacg
     3541 cctattgcca ggacaacgac atctcgtggt tcgactgggg tcagagagac gaggaactcg
     3601 cccttctcgg attcacgcgc cgggtcatgg agttccgtcg gaggcatccg accttccgac
     3661 gccgtcgatg gttcaccggc cggcagatct tcggctccga ggtctcggac gtcggctggt
     3721 tcaaccccga cgggtccgag atgacgagcg aacaatggaa ccaaggtttc gccaagtcca
     3781 tcggggtatt tcctgaatgg agatgagatc cccgaaccgg acgagaaagg cgagcgcgtg
     3841 accgacgaca gcttcctgct gctgttcaac gcgcattccg aggacgtgcc gttccgcctt
     3901 ccctccgatc gctgggggga ggcgtggacg gccgcagtcg acacgttcga tcctgcactc
     3961 gaggagggct ccctcaccta caaggcgggc gacgaggttc cggtccggtc gcgctcgatc
     4021 gtggtgttgc gccgtgccga gtaagcatct tcgggctacc taccgcgttc aggtgcacaa
     4081 ggacttcacc ctggatcgcg ttgccggact gaccgactac ttcgccgaac tgggattcag
     4141 ccacgtctac tgctctccct acctgtcgtc gcgaccgggc agcctgcacg gctacgacgt
     4201 cgtgaaccac tcgaccgtgg acgatgagct gggtggggcg gctgcactcg agcgcctcat
     4261 ggctgccctc gaagcgaaac agatgggaca catactcgac gtcgttccaa accacatgac
     4321 gatcgccgag cgggcgaacg agtggtggtg ggacgtcttg cgcaacggtc cctacagcga
     4381 gtacgcaacc ttcttcgatg tcgactggga tccgcccgag gcccgtctgc gccggaagat
     4441 actgatcccg atcctcggcg accactacgg ccgcgtactc gagaagggcg agatcaaggt
     4501 cgaggaggag gccgacgagt tcgtcgtccg ttactacgac cacgtcctcc cggtagcacc
     4561 ggactcgatc tcgtatgcat tcgcggagga gggcgcccac gacatccgtc gtatcaacga
     4621 cgatgctgcg ctgctccact ctctcttgga gctacagcac taccgcctcg cttactggaa
     4681 gaccgcaggc aaggagctca actaccgcag gttcttcgcg atcaacgacc tcgccgcgtt
     4741 gcgtgtcgac agggacgacg tcttcgacc
//
LOCUS       DATMED010000076         4320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3504763, whole genome shotgun sequence.
ACCESSION   DATMED010000076 DATMED010000000
VERSION     DATMED010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504763"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..526)
                     /gene="purE"
                     /locus_tag="VNP73_01920"
     CDS             complement(62..526)
                     /gene="purE"
                     /locus_tag="VNP73_01920"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWL64704.1"
                     /translation="MGALVGIVMGSDSDMPVMEKAGAVLDRFEIDWEIEVMSAHRNPG
                     RVSEYASTAAERGLKVIIAGAGLAAHLPGVVAAHTHLPVIGVPLFQEGLNGVDSLYSI
                     VQMPPGVPVAAVAIGGSKNAGLLAVQMLALEDSALAESLKEFKLKMVAGEKL"
     gene            complement(526..1803)
                     /gene="purD"
                     /locus_tag="VNP73_01925"
     CDS             complement(526..1803)
                     /gene="purD"
                     /locus_tag="VNP73_01925"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWL64705.1"
                     /translation="MRFLLLGSGGRESALAWGLSRSSVVSELVAAPGNPGIGALAQLE
                     EIDIEDVDAITTLARRIRPEVVVVGPEVPLVAGAADSLRAEGIDVFGPEAAAARIEGS
                     KRYAKELMERGGIPTAAWRSFEDFDAAVSYLDEVGSPYVIKADGLAAGKGVVVTEDRD
                     AAIAGLKERLVEGVFGRAGSRVVIEEFLDGQEASLIAFTDGTTVIPCEPAQDYKRAFD
                     ADAGPNTGGMGSYSPVPACPPQVAQEIIEEVIVPMVRTLSAAGTPFVGALYAGLALTS
                     KGPRVVEFNARFGDPETQALIPRLDSDLGEVCAAAARRELDGVSVSWRDEACVTVVLA
                     SHGYPGSYKTGFEISGLEEVGRMEDVTVFHAGTSRDDAGPVRTAGGRVLAVSALGKTF
                     KEARARAYDAAGTIDFEGKHLRTDIAARAEGSD"
     gene            complement(1800..3083)
                     /locus_tag="VNP73_01930"
     CDS             complement(1800..3083)
                     /locus_tag="VNP73_01930"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWL64706.1"
                     /translation="MPGIALVGTQWGDEGKGKVTDLLAEKTDLVVRYQGGNNAGHTIV
                     VEGERYALHLVPTGILYPHCIPVIGPGVVVNPSVLIEELDTLEERGIETGRLLLSGNA
                     HLIMPYHLELDRVTERRLGKNRLGTTKRGIGPAYADKATRIGLRVQDLLDPKIFSAKL
                     EVALKEKNLLLTRVYGRLPLEQKEIEGDYLAFADRLGPHITDTDRVVQGALDEGKTVL
                     FEGAQATLLDLDHGTYPFVTSSNPIAGGACAGAGVGPRDIEQIIGISKAYCTRVGTGP
                     FPSEADPADGEILVEAGQEYGTTTGRKRRCGWFDVVGARFAARLNTLTELVITKLDVL
                     SEFERIKVCVGYSYEGKRYDHMPPNQTIFNKCRPIYEELPGWSQDITGARSLEDLPKE
                     ARSYLEMLENLVDTPISWVSVGPSRDQIVRLRDAP"
     gene            complement(3101..3304)
                     /locus_tag="VNP73_01935"
     CDS             complement(3101..3304)
                     /locus_tag="VNP73_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWL64707.1"
                     /translation="MGVIKTIDLVGVSTESWRDAATQALAEAAKTIRGISGMEVLDTS
                     CNVADGKITEYLTHVRIKFRIER"
     gene            3411..>4320
                     /locus_tag="VNP73_01940"
     CDS             3411..>4320
                     /locus_tag="VNP73_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64708.1"
                     /translation="MPQIDPGIFQLFVLGLLVVGVLVLVATLLTMSRIRDVLRDRPGG
                     EARTRSGSSIAPSRRENRELARTERALESTRSEQSAVTSSLEAARRERDREVEALSAA
                     RAELDRERRELATAREATAEARSELDKSRAERNQAETEATTLQQERTKEQAALQAAWK
                     ERDGHRSSTDQIRREQKELEQSAADIRQELASEEAAAKATKDELAALQKELAAAKEIT
                     NDAEAELQKVRKEKAGAQLTVEEAKTQRTEEQTVVQTLWKERDAQRAAIDTARRERTE
                     MEASLHETQQEVEQEEAALDAARLELE"
BASE COUNT          834 a         1429 c         1311 g          746 t
ORIGIN      
        1 ctaacctcga acttgtcgcg tcaaagcgcg ctggaacgtt actcagttag ccaagaaggg
       61 cctagagctt ttcgcccgcc accatcttta gtttgaactc tttcaaactc tcggcaaggg
      121 ccgagtcctc gagcgcgagc atctggaccg cgagcagacc cgcgttcttc gagccgccga
      181 tggcaactgc cgcgaccgga acgcccggtg gcatctgcac gatcgagtaa agagagtcga
      241 cgccgttcaa cccttcctgg aacagcggca caccgatgac cggaagatgt gtgtgcgccg
      301 cgacgacgcc gggaaggtgc gcggccaaac cggccccggc gatgatgacc tttagtccac
      361 gctccgccgc ggtcgacgcg tattcgctaa cacgacccgg attgcgatgt gcggacatca
      421 cctcgatctc ccaatcgatc tcgaaccgat cgaggaccgc tccggccttt tccatcaccg
      481 gcatatcgga atcgcttccc atcacgatcc ctactagtgc acccatcagt cgcttccttc
      541 cgctctagcc gcgatgtcgg ttcgcaaatg cttgccttcg aagtcgatcg tcccggcggc
      601 atcgtaggcc ctagcgcgtg cctccttgaa agtcttgcct agagcgctaa cggcgagcac
      661 ccggcccccc gcggtgcgca ctgggccggc gtcgtctcga gaggtcccgg catggaacac
      721 ggtgacgtct tccatacgtc ccacctcctc cagacccgag atctcgaagc ccgtcttgta
      781 ggaccccggg taaccgtgag atgcaagcac aacagtcaca caggcttcgt cgcgccacga
      841 cacgcttaca ccgtcgagct cccgtctcgc cgcagcagca cagacttcac caagatccga
      901 atccagtcgc gggatgagcg cttgggtctc gggatcgccg aagcgagcgt tgaactcgac
      961 gacgcgaggg cccttcgacg tcagtgcgag accggcataa agggctccga cgaaaggtgt
     1021 cccggcagcc gagagcgtcc tcaccatcgg aacgatgacc tcctcaatga tctcttgggc
     1081 cacctgcggt ggacacgcgg gcacgggact gtatgagccc attcccccgg tgttgggacc
     1141 ggcgtcggca tcgaacgccc tcttgtagtc ctgcgcgggt tcgcacggga tcacggtggt
     1201 gccgtcggtg aatgcgatca gtgaagcctc ctgcccatcg aggaactcct cgatcacgac
     1261 acggctgccc gcgcgtccaa agacaccttc aacgagtcgc tccttcagcc cggcgatcgc
     1321 ggcatctctg tcttcggtca cgacgacgcc cttgccggcc gcgagcccgt ccgccttgat
     1381 gacgtaagga gagccgacct cgtcgaggta cgacaccgct gcatcgaagt cctcgaacga
     1441 tcgccacgca gccgtcggga tcccgccgcg ctccatcagt tctttcgcgt atctctttga
     1501 tccctcgatc cgtgctgccg ccgcctccgg accgaacacg tcgatcccct ccgcgcgcag
     1561 agagtccgcc gcgcctgcga ctaagggaac ctcggggccg acgaccacga cctccggccg
     1621 gatcctgcgc gcgagggttg tgatcgcgtc gacgtcctcg atatcgatct cttcgagctg
     1681 cgcgagcgcg ccgatgcccg ggttacccgg tgcggcaaca agctccgaga ccacactgga
     1741 cctggacaat ccccaggcga gcgcgctttc gcgcccgccg gagccgagga gcaagaacct
     1801 cacggagcgt cccgcaggcg gacgatctgg tctcggctcg gcccgaccga tacccacgag
     1861 atcggcgtgt cgacgaggtt ctccaacatc tccaggtacg agcgcgcttc cttagggaga
     1921 tcctcgaggg aacgagcacc cgtgatgtct tgcgaccacc cgggcagctc ctcgtagatc
     1981 ggccggcact tgttgaagat cgtttggttc ggaggcatgt ggtcgtagcg cttgccttcg
     2041 taggaatacc cgacgcagac cttgatgcgc tcgaactcgg acagcacgtc cagcttcgtg
     2101 atcacgagct cggtcaaggt gttgaggcgc gcggcgaaac gagcccccac cacgtcgaac
     2161 catccacaac gacgcttccg gccggtggtc gtgccgtact cctggcccgc ctcgacaagg
     2221 atctcgccgt ccgcgggatc ggcctcggat gggaacgggc ccgtcccgac gcgcgtgcaa
     2281 taagccttgg atatcccgat gatctgctcg atgtcgcgcg gcccgacgcc ggcccccgcg
     2341 cacgcgccgc ccgcgatcgg gttcgacgaa gtcacgaagg gataggtgcc gtgatcgagg
     2401 tcgagcaagg tcgcttgcgc gccttcgaac agaacggtct tgccttcgtc gagtgctccc
     2461 tgaaccactc gatcggtgtc tgtgatgtgg ggaccgaggc ggtcggcgaa cgcgaggtag
     2521 tcgccctcga tctctttttg ctcgagaggc agccggccgt agaccctcgt caacaagaga
     2581 ttcttctctt tcagcgcaac ttcgagcttc gcagagaaga tcttcggatc gagcaggtcc
     2641 tgcacacgca acccgatgcg cgtcgccttg tcggcatacg caggtccgat gccgcgcttg
     2701 gtggttccga gccggttctt gcccaacctt ctttccgtga cccgatccag ttcgaggtgg
     2761 tacggcatga tcagatgcgc gttcccggag aggagcaggc gacccgtttc gatcccgcgc
     2821 tcctccaacg tgtcgagctc ctcgatgagg acactggggt taacgacgac gcccgggccg
     2881 atcaccggga tgcagtgcgg atacaggatg ccggttggaa ccaggtgcag cgcgtagcgc
     2941 tcaccttcga ccacgatcgt gtgcccggcg ttattgcccc cctgataacg gaccacgagg
     3001 tccgtcttct cggcgagcag atccgtgact ttgcctttgc cttcgtcacc ccattgggtt
     3061 ccgacgaggg cgatcccggg catctagatg ggcttccgcg tcaacgttcg atcctgaact
     3121 tgatccgaac gtgagtcagg tactcggtga tcttgccgtc cgccacgttg caggaagtgt
     3181 ccaggacctc catgcccgag atacccctga tcgtcttggc tgcctctgcc agcgcttgag
     3241 tcgctgcgtc acgccacgat tccgtcgaca cgccgaccag atcgatggtc ttgattaccc
     3301 ccacaagcac acctctttca gggtgatgga gtagccgggc aacgcttgcg cgttcgattc
     3361 tataaagggc tgccgttcac agcggtttca gggatgatgt atcgctaacc atgcctcaga
     3421 tcgaccccgg gatattccaa ttattcgtac tcggcctatt ggtcgtgggc gtcctcgtgc
     3481 tcgtggcgac gctgttgacc atgagccgga tacgtgacgt cttgcgcgat cgtcccggag
     3541 gcgaggcacg gacacgttca ggttcctcga tcgctccctc ccgccgggag aaccgagaac
     3601 tcgctcgaac cgagcgtgcg ctggagtcaa cccgaagcga acagagcgcg gtcacctcgt
     3661 cgcttgaggc cgcacggcgc gaacgagacc gagaagtaga ggccctcagc gccgcgcgcg
     3721 cggaactcga cagggaacgt cgggagctgg ccacagcccg ggaggcgacg gccgaggccc
     3781 gttcagagtt ggacaagtcg cgcgcggaac gcaaccaagc cgagacggaa gccaccacgc
     3841 tccagcagga gcggacgaag gagcaagctg ccctccaggc cgcgtggaag gaacgcgacg
     3901 gccaccgatc gtcgacagac cagattcggc gcgagcagaa agagctcgag caatcggcgg
     3961 cggacatcag gcaagagctt gccagcgagg aagctgcggc caaggcgact aaggacgagc
     4021 ttgccgctct gcagaaggag cttgccgcag ccaaggaaat aaccaacgac gccgaagccg
     4081 agctccagaa ggtccgcaaa gagaaggcgg gcgctcaact gacggtggag gaggccaaga
     4141 cccagcgaac ggaggagcaa accgtcgtac agacgctctg gaaggagcgc gatgcgcagc
     4201 gcgcggccat cgacacggcc cggcgcgagc gcaccgagat ggaggcatcg ctacacgaga
     4261 cgcaacaaga ggtcgaacaa gaagaggccg cgctcgacgc ggcccgactc gaactggaac
//
LOCUS       DATMED010000077         6087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4277364, whole genome shotgun sequence.
ACCESSION   DATMED010000077 DATMED010000000
VERSION     DATMED010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277364"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VNP73_01945"
     CDS             complement(<1..307)
                     /locus_tag="VNP73_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64709.1"
                     /translation="MRRRVCLSGFVFVLLATTVVPAASAAQPKCRGQVATKVGTPGDD
                     ILVGTARNDVIVGLGGRDEIIGNKGNDLICGGGGDDVWLNGAFGKDRVFGQGGADKII
                     "
     gene            413..1669
                     /locus_tag="VNP73_01950"
     CDS             413..1669
                     /locus_tag="VNP73_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWL64710.1"
                     /translation="MGTVLYLAPGVKRERKTKTPTNSSNKVALVLGGGGITGAAYHLG
                     VLNAMNAMSPERSVNDFDLYVGTSAGAVIGACLANGITPEDMILANLGHESTVPAIGA
                     DEIMQPDRIGLVRSMVRWPFGLLGALRRYAGHPFTTSLIDGLGAVAEGLPPALYTTDG
                     TERYVRQLLETDGRSNRFEEVKRKLLITATDIDAARRVVFGAQEGPEAEISEAAAAST
                     AIPLLYAPRRIGNRVYYDGGLRSTTNIDVAIGHGARLVICINPLVPYVHDIRHLLPTA
                     TGLDARHISEKGFPHIAAQTFRIMAQALVEKEIELISHAHPDVDIILIEPRSDDEHMF
                     IFNLMDYGSRERVARHAFETVAIDLVTRYPDYKKVLGRHGIKISRDLLIDQLQNVVEG
                     GEPRFFGASGETAKKRLAPGDERASS"
     gene            complement(1626..4547)
                     /gene="uvrA"
                     /locus_tag="VNP73_01955"
     CDS             complement(1626..4547)
                     /gene="uvrA"
                     /locus_tag="VNP73_01955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWL64711.1"
                     /translation="MSGIPSSSSAGKLVVRGAREHNLKNVSLELPRDSLIVFTGISGS
                     GKSSLAFDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAISIDQKSASRN
                     PRSTVGTITEVYDYLRLLYARIGRPHCHNCGRPIARQTPEQIVDQVLELQEGTKFQVL
                     APVVRSRKGEFVGLLEDLSRKGYVRARIDGEVHELSEKIKLDRYKQHDIDIIVDRLVI
                     KEGIASRLTDSIETALKLAEGTAAIDLVEDESEDILFSQHFACTFCGLSFDELAPRNF
                     SFNSPYGACERCDGLGTHLEVDPELVIPNPELSIEEGAIGPWSGRTLEYFEKLLQSAA
                     KEFRFKTDVPFYKLSARAQDIIMYGSEDVQVHVRYKNRFNRIRSYWTTFEGVISWLER
                     RRDETDSDHARERYEQYFREVPCPSCKGARLMPQSLAVTVGGKNIFDLTNSCIGDIDN
                     FLRQVELNDRETTIAERVLKEIHARLGFLLDVGLDYLTLARASATLAGGEAQRIRLAT
                     QIGSGLVGVLYILDEPSIGLHQRDNKRLIDTLVRLRDLGNTLVVVEHDEETIRSADHV
                     VDIGPGAGELGGKIVYSGAFDGLLETSESVTGDYLSGKRTIPIPTNRRGPDRGHITIR
                     GARQHNLKNLDVSIPIGCFVSVTGVSGSGKSTLVNEVLYRAAHQHLIRTARLVPGKHK
                     KIEGLDKIDKVIDIDQSPIGRTPRSNPATYTGVFDHIRKLFAKTPDAKRRGYQPGRFS
                     FNVRGGRCEACSGEGTIKIEMHFLPDVYVPCEVCKGKRYNRETLEVRFKGKNISEVLD
                     MSVSEGCEVFANIPPIARHLNTLRDVGLGYIRLGQPAPTLSGGEAQRVKLSTELSKRS
                     TGQTLYILDEPTTGLHFDDVAKLLGVLHRLVDAGNTVVVIEHNLDVIKTADHLIDLGP
                     EGGAGGGEVVAVGTPEEVAATRGSYTGEYLKELLVKPSQKKKRAAATKTRARRLERAA
                     S"
     gene            4615..5181
                     /locus_tag="VNP73_01960"
     CDS             4615..5181
                     /locus_tag="VNP73_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64712.1"
                     /translation="MKRMPFLLALVLALVLALGACAGGGGAEEEAAPRTFEGHGVTFD
                     YPAEWERIDDTTTSASAGNQIWNESYGLDATNLVAVSLYTIGAKITPKNINGFEGEIT
                     AAIKGVVEQAGGSLEGEPAALTVDGLPTLDYRATAITTVDDATVDSRIVLMFDGADEY
                     FLNCQYSEQERRAVEDACDLVLDTFSVE"
     gene            complement(5182..>6087)
                     /locus_tag="VNP73_01965"
     CDS             complement(5182..>6087)
                     /locus_tag="VNP73_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64713.1"
                     /translation="YGSRPYGRVWRVEDTDKDGVGDKQTLLIGDLPNGRHNTNGMAIG
                     PDGMLYVSNGNATDDGVEGGQPEVEPWSGSVVRLDPEGEKLSIAELPRKKTLVASGMR
                     NLYDVVFSPFDPTQIFIPMNGLDDARKQEDEGDEAGRENSDDLLFRADIDDRRNGRAH
                     REDFGFPSCLYNVVKKGNLKPYDNPNPRTIRKFGPCPKKKIVRPETSFGLHVSADGAA
                     FQLGDAWGDDYRNDLFVAEWGNLFGTEVRGHDVVRVEFNDTGSKVTGQSQFTVGGAPL
                     DVTFDDAGNLYVLDFSGDVFRIAKV"
BASE COUNT         1121 a         1951 c         1819 g         1196 t
ORIGIN      
        1 ggatgatctt gtcagcaccg ccctgaccga agactcggtc cttgccgaag gcaccgttca
       61 accagacgtc gtcgccgccg ccgccgcaga tgagatcgtt acctttgttg ccaattatct
      121 cgtcccgacc acccagcccc acgatcacgt cgttgcgcgc cgttccgacc aatatgtcgt
      181 cgccgggagt acccaccttc gttgccacct ggccgcggca cttgggttgg gcggcggaag
      241 cagcgggcac cacagtggtg gcaagcaata cgaacacgaa cccggacaga cagacacgtc
      301 gtcgcattag ggctccaaat atgggaaacc tgatcatctt gaagcgtagg gagcctgcgg
      361 gccccggtct aggggcttcg agttctttct ctcgtaggta ggctggccgg cgatgggcac
      421 cgtcctctac ctcgctccgg gcgtgaagcg tgagcgcaaa acaaagacac ccaccaactc
      481 ctccaacaag gttgctctcg ttctcggcgg cggtggcata accggggccg cctaccacct
      541 gggcgtgctc aacgcgatga acgcgatgag ccccgagcgt tccgtgaacg atttcgacct
      601 ctacgtcgga acgtcggcag gcgcggtgat cggcgcgtgc ctggccaacg gcatcacgcc
      661 ggaagacatg atcttggcca acctcggtca tgagtcgacc gtgcccgcca tcggagccga
      721 cgagatcatg cagccggatc ggatagggct ggtccgatcg atggtccggt ggccgttcgg
      781 tctactgggc gcgttgcgcc ggtacgccgg ccaccccttt acgacctcgc tcatagatgg
      841 cctcggtgcg gttgccgaag gcctccctcc ggctctctat acgaccgacg gcaccgagag
      901 atacgtacgg cagctgctag aaaccgacgg gcgctccaac cgattcgaag aggtcaaacg
      961 caagctactg ataaccgcca ccgacatcga cgctgcacgt cgagtcgtct tcggggctca
     1021 ggaagggccg gaggcggaga tctcggaggc ggccgctgcc tcgacggcca tccctctgct
     1081 ctatgcgcct cgtcgcatcg gcaaccgcgt ctattacgac gggggtcttc gttcgacgac
     1141 gaacatcgac gtcgcgatcg gacacggtgc gcgcctggtg atctgcatca acccactggt
     1201 cccctacgtc cacgacattc gtcaccttct ccccacggcg accggcctcg atgcccgcca
     1261 catctcggag aagggcttcc cgcacatagc cgcccagacc ttccggatca tggctcaggc
     1321 attggtcgaa aaggagatcg agttgatctc gcatgcgcat cccgacgtgg acatcatcct
     1381 gatcgagccc cgctcggacg acgagcacat gttcatcttc aacctgatgg attacggatc
     1441 acgagagcgc gtcgcgcgcc atgcattcga gacggtggcg atcgatctcg taactcgata
     1501 cccggactac aagaaggtgc tcggccgcca cggcatcaag atcagccgcg acctactgat
     1561 cgatcagctg cagaacgtgg tcgagggcgg cgagcctcgc ttcttcggag cgagcggaga
     1621 aacggctaag aagcggctcg ctccaggcga cgagcgcgcg tcttcgtagc agccgctctc
     1681 ttcttcttct gagacggctt cacgagtagc tctttcagat actcaccggt gtacgagccg
     1741 cgcgtagctg cgacctcttc cggagtaccc acggcaacaa cctctccccc gccggctccg
     1801 ccttcgggtc ctagatcgat caggtgatcc gccgtcttga tcacgtcgag gttgtgctcg
     1861 atgacgacga cggtattgcc ggcatcgacc aatctgtgca gcactcccag aagcttcgca
     1921 acgtcgtcga agtgcaaccc agttgtgggt tcgtcgagga tgtagagcgt ctgccccgtc
     1981 gagcgcttgg acaactcagt cgatagcttg acgcgctgcg cctcgcctcc cgacagtgtc
     2041 ggtgcgggct gtccaaggcg gatgtagccg agacccacat ccctcagcgt gttgaggtgg
     2101 cgagcgattg gcgggatgtt cgcgaacacc tcgcacccct ccgacacgct catgtcgagg
     2161 acctcggaga tgttcttgcc cttgaaacgc acttcgagcg tctcccggtt gtagcgcttt
     2221 cccttgcaca cctcgcacgg cacgtagacg tcgggaagga agtgcatctc gatcttgatc
     2281 gttccctcgc ccgagcacgc ctcgcaccga ccgcccctca cgttgaacga gaacctgccc
     2341 ggctggtagc cccttctctt ggcgtcgggg gtcttggcga agagcttgcg gatgtgatcg
     2401 aagacgccgg tgtaggtagc ggggttggac cgaggagtcc gcccgatcgg cgattgatct
     2461 atatcgatga ccttgtcgat cttgtcgagg ccctcgatct tcttgtgctt accgggaacc
     2521 agcctggccg tgcggatcag atgctggtga gcggctcggt acaagacctc gttcaccagg
     2581 gtcgacttgc cggaacccga gacgcccgtc accgacacga agcatccaat cgggatcgac
     2641 acgtcgaggt tcttcaggtt gtgctgccgt gcacccctga tcgttatgtg gccgcggtcc
     2701 ggaccacgac gattcgtcgg tatcgggatc gtcctctttc cggaaaggta gtcgcccgtg
     2761 accgattccg atgtctcgag cagaccgtca aacgctcccg agtaaacgat cttgccgccg
     2821 agctcccccg cgccgggacc gatgtccacg acgtggtcgg ccgatcggat cgtctcctca
     2881 tcgtgttcga cgacgacgag cgtgttgcct aggtcgcgca gccggaccag cgtgtcgatc
     2941 aatcgcttgt tgtcgcgctg gtgcagtcct atcgacggct cgtccaggat gtagagaacc
     3001 ccgaccaggc ccgagccgat ctgcgtcgcg agtctgatcc tctgcgcctc cccgcccgca
     3061 agtgtcgccg aggcgcgggc gagcgtcagg tagtcaagac cgacgtccag caagaagccg
     3121 agccgcgcat ggatctcctt cagaacgcgt tctgcgatcg tcgtctcgcg atcgttaagc
     3181 tcgacctgcc gaaggaaatt gtcgatgtct ccgatgcacg agttcgtcag atcgaagatg
     3241 ttcttgccac caacggtcac ggcgagcgac tgaggcatca agcgcgcgcc cttgcaggac
     3301 gggcacggca cttctcggaa atattgctcg taacgctcgc gggcgtggtc cgagtcggtt
     3361 tcgtcgcgcc gacgttcgag ccagctgatg acaccctcga acgtcgtcca gtacgaacgg
     3421 atccggttga agcggttctt gtagcggacg tgaacctgca cgtcctccga gccgtacatg
     3481 atgatgtctt gagccctcgc agacagcttg tagaagggca cgtcggtctt gaagcggaac
     3541 tcttttgccg cggactgaag aagcttctcg aagtactcga gcgtccggcc ggaccacggc
     3601 ccgatcgctc cctcctcgat cgacagttcc gggttcggga taacgagctc gggatcgacc
     3661 tctaggtgag tgccgagacc gtcgcacctc tcacacgctc cgtacggtga gttgaacgag
     3721 aagttcctgg gagccagctc gtcgaaggac aaaccacaga acgtgcacgc gaagtgctgg
     3781 ctgaagagga tgtcctccga ctcgtcctcg acgaggtcga tcgcggcggt cccctcggcc
     3841 agcttcaatg cggtttcgat cgaatcggta aggcggctgg ctatcccctc cttgatcacg
     3901 agtcggtcga cgatgatgtc gatgtcgtgc tgcttgtaac ggtcgagttt gatcttctct
     3961 gaaagctcgt gaacctctcc atcgatccgc gcccggacgt acccctttcg ggaaaggtcc
     4021 tccagcagtc ctacgaactc gcccttacga ctgcgcacaa cgggcgccag cacctggaac
     4081 ttggtgccct cctgcagctc cagaacctga tccacgatct gctcgggtgt ctggcgggcg
     4141 atcggccgcc cgcagttatg gcagtgaggc ctcccgatcc gggcgtagag aagcctgagg
     4201 tagtcgtaga cctcggtgat cgtcccaacg gttgaacgag ggttccggga cgccgacttc
     4261 tgatcgatcg agatcgccgg agacaggccc tcgatgaagt ccacgtccgg cttgtccatc
     4321 tgcccgagga actggcgggc gtaggccgat agagactcca cgtaacggcg ttgcccctcg
     4381 gcgtagatcg tgtcgaatgc gaggctggat ttgcccgatc cggagatccc cgtgaacacg
     4441 atcaaggagt cccgagggag ctcgagactc acatttttga ggttgtgctc tcttgctcca
     4501 cggacgacga gcttgcccgc cgagctgctc gaggggatcc cagacatggc catgtcaccc
     4561 taaacgacgc ccttgtaact taacttcccg ttttccgcga gctaccctga cccgatgaag
     4621 cggatgccct tcttgcttgc cctggtcctt gccctggtcc ttgccctggg cgcctgcgcc
     4681 ggtggtggtg gagccgagga ggaagcggcc ccccggacat tcgagggcca cggggtcacc
     4741 ttcgattacc ccgccgagtg ggagcggatc gatgacacga cgacgagcgc gtccgcgggg
     4801 aaccagatct ggaacgagtc ctacggcctg gacgccacga atctggtcgc ggtgagcctc
     4861 tacacgatcg gggccaagat cacgcccaag aacatcaacg gcttcgaggg cgagatcacg
     4921 gccgctatca agggggttgt agagcaggcc ggcgggagcc tcgaaggcga accggccgcg
     4981 ctgactgtgg acgggctgcc tacgctcgac tatcgggcaa cggcgatcac gacggtcgac
     5041 gatgccacgg tcgacagccg catagtgctg atgttcgatg gcgccgacga atacttcctc
     5101 aattgtcagt actccgaaca ggagcgtaga gcggtcgagg acgcgtgcga cctggtcctc
     5161 gacaccttct cggtcgagtg atcagacctt agctatccga aacacgtctc cactgaagtc
     5221 caaaacgtag agattccccg catcgtcgaa cgtcacgtcc aacggagctc cccccacagt
     5281 gaattggctc tgcccggtga ccttcgagcc cgtgtcgttg aactcgaccc gcacgacgtc
     5341 gtggccccgc acctcggttc cgaagaggtt cccccactcg gccacgaata ggtcgttgcg
     5401 gtagtcgtct ccccacgcgt cgccgagctg gaaggccgcg ccgtcggccg acacgtggag
     5461 accgaagctc gtttctggac ggacgatctt cttcttgggg cagggcccga acttgcggat
     5521 cgtccgaggg ttcggattgt cgtacggctt caggttgccc tttttgacca cgttgtagag
     5581 gcatgagggg aacccgaaat cctctcggtg cgcgcggccg tttctcctgt cgtcaatgtc
     5641 ggcccggaag agaagatcgt ccgagttctc tcggcccgct tcgtcacctt cgtcttcctg
     5701 cttgcgggcg tcgtcgagcc cattcatcgg gatgaagatc tgcgtcggat cgaacggaga
     5761 gaagacgacg tcgtagagat tgcgcatccc gcttgcaacc agcgttttct tgcgcgggag
     5821 ctcggcgatc gagagcttct caccctcggg atccaaccga acgaccgatc ccgaccaggg
     5881 ctcgacctcg ggctggccgc cctccactcc gtcgtcggtt gcgtttccat tcgatacgta
     5941 gagcatcccg tccggcccga tcgccatacc gttcgtgttg tgacgaccgt tcggaaggtc
     6001 accgatcaac agtgtctgtt tgtcaccgac gccatccttg tcggtgtctt cgacacgcca
     6061 cacccgtccg tacggtctcg acccgta
//
LOCUS       DATMED010000078         3986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1985050, whole genome shotgun sequence.
ACCESSION   DATMED010000078 DATMED010000000
VERSION     DATMED010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985050"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VNP73_01970"
     CDS             complement(<1..1119)
                     /locus_tag="VNP73_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HWL64714.1"
                     /translation="MRPIRLILHTHWDREWYQPFERFAERLVPTIDLVLDTMRSDPRL
                     THFHLDGQLALVDDYLARRPDRTDELRSLIAEGRLSCGPWITLVDEFLVSGESIVRSL
                     DDGLRRGDALGGALRVGYVPDQFGHVGQLPQILANAGIGTAVAWRGVPAAVETGEFLW
                     RSPDGSEVLVAYMPFGYAQGARLESTEGDFVKRLQRELVRSESWLRDGQPLMLMTGDD
                     HEPMNPHIPELVDHARARGVDVAISSLKDSVARAPAADSPVVVGELRSAARANLLPNT
                     YSVRPHQKIERAGAELILERYAEPLAALVPGFEWPAEALAEAWHLLHLNGAHDSVCGC
                     STDEVAHAVDERTRAARAIANRVAHAAFDVLREEVDGDG"
     gene            complement(1159..1953)
                     /locus_tag="VNP73_01975"
     CDS             complement(1159..1953)
                     /locus_tag="VNP73_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWL64715.1"
                     /translation="MGQPMTSEYNYSGRSDPKRSLELLWRLEIKPSRGPKPGLSLDQI
                     VAAAIEVADAEGLRALSMRRVAEHLGVGTMSLYRYVPAKAELLDLMLDAVDAGLERPD
                     ASVVGWRSKLEFFARESLAGYHRHPWVLHIVAMDARPPLGPNILSSFEAMLEAVAGIG
                     LKGSEMIAVIKLVQGYVQGVARSSIEAIQVVEHTGVSEGQWWSERTEFWENIFDVERF
                     PTITEVWESGGFNEPEDDFEFGLTRVLDGIEVLVAERQQTGQGKAK"
     gene            2032..2994
                     /locus_tag="VNP73_01980"
     CDS             2032..2994
                     /locus_tag="VNP73_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL64716.1"
                     /translation="MGGDFAVVAEGLRKSYGKTLALDGFDLAVPSGSVCGLLGPNGAG
                     KTTAVRILGTLLRFDDGRAEVGGIDVRRDPAQVRSRIGLTGQNPAVDEILTGRQNLVM
                     FGKLNHLDAESARRRADELLEQFGLADAAGKQAKSYSGGMKRRLDLAASFILAPQVMF
                     LDEPTTGQDPRNRNEVWNVVRELVAGGTTVLLTTHYLEEADQLADQISVIDRGRVIAD
                     GTPEQLKSKIGGSRVDIVLNNVDELPIVAGLVERVCGGEAEVDHDRRRVSAPVKDRVA
                     TLTEVLRALEDAGILAEDVAVRKPTLDEVFLELTGHRADEEVAA"
     gene            2991..3821
                     /locus_tag="VNP73_01985"
     CDS             2991..3821
                     /locus_tag="VNP73_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL64717.1"
                     /translation="MTPASALHPQTYTARLKWAVKDGWTVAHRDLSHWAHQPGQIVAG
                     LIFPIVFILLFGYVFGSGMIPPEGGDYIDFLMAGLFAQTMAFGIGETMVAVNTDADKG
                     ITDRFRSLPMAQSAVVVGRNTADMLNSATGLIVMIACGLVVGWRWHEGIGNALLAVGL
                     LLLLRFAFLWIGIYIGLVVKSPEALGAVWGLLFPVTMLTNAFVDPRFMPDWLGTIAEW
                     NPISSTISATRDLFGNAGASAGGTWIAENAMMMAVVWPLLIVVVTLPLAVRRYQNLSR
                     "
BASE COUNT          720 a         1276 c         1270 g          720 t
ORIGIN      
        1 cccgtcgccg tcgacctcct ctcgcaggac gtcgaaggcc gcatgggcaa ctcgattcgc
       61 tatcgctcga gccgcgcgcg tccgttcgtc gacggcatgc gccacctcat cggtagaaca
      121 gccgcagacc gagtcgtggg ctccgttcag gtgcagtagg tgccaggcct cggccagagc
      181 ttccgccggc cactcgaacc ccggaaccag cgcggcaagt ggttccgcgt agcgctcgag
      241 gatcagttcg gcccccgcgc gctcgatctt ctgatgaggc ctgaccgagt aggtgttcgg
      301 tagcagattc gcgcgcgctg ccgaacgcaa ctcgccgaca acgacgggtg agtctgcggc
      361 cggagcgcgc gctacggaat ccttcaagga cgagatcgcc acgtcgacgc cgcgcgcgcg
      421 tgcatgatcc acgagctccg gaatgtgcgg gttcatcggc tcgtggtcgt caccggtcat
      481 cagcatcaga ggttgtccgt cgcgcagcca cgactcactg cggacgagct cgcgctggag
      541 tcgcttcacg aaatcccctt cggttgattc caggcgcgcg ccttgcgcgt agccgaacgg
      601 catgtaggcc accagcacct ccgagccatc gggtgacctc cagaggaact cgcctgtctc
      661 gacggccgcc gggacaccgc gccacgcaac ggctgtgccg attcccgcgt tcgcgagaat
      721 ctgaggaagt tgaccgacgt gaccgaactg atcgggtacg tagccgactc gtaacgctcc
      781 cccaagggcg tctccccggc gcagtccgtc gtccagtgag cgcactatcg attcacccga
      841 gacaaggaac tcatcgacaa gcgttatcca ggggccgcag gacaaccggc cctcggcgat
      901 cagggatcga agctcgtcgg ttcggtcggg acggcgcgcg agatagtcgt cgacaagcgc
      961 gagctgaccg tcaaggtgga agtgcgtgag cctcggatcc gaccgcatcg tgtcgagcac
     1021 gagatcgatg gtcgggacca gccgttcggc gaagcgctcg aatggctgat accactctcg
     1081 gtcccagtgc gtgtggagaa ttagtcgaat cggacgcata gcaagcaaac tatggcaaga
     1141 aatttcaggt ccggcctact acttggcctt gccttggcca gtctgctgtc gctcggcgac
     1201 gaggacctcg ataccgtcca gcacccgcgt gaggccgaac tcgaagtcgt cctcgggttc
     1261 gttgaaacct cccgactccc agacctcggt tatcgtcggg aacctctcca cgtcgaagat
     1321 gttctcccag aactcagttc gctccgacca ccactgcccc tcgctcaccc cagtgtgctc
     1381 cactacctga atcgcttcga tggagctgcg tgcgactccc tggacgtagc cctggaccag
     1441 cttgatcacg gcgatcatct cgcttccctt gaggccgatc cccgcgacgg cttccagcat
     1501 ggcctcgaac gaggagagga tgttcggccc caacggcggc cgggcgtcca tggccacgat
     1561 gtgcaagacc caggggtgcc ggtgataacc ggccaggctc tcccgcgcga agaactcgag
     1621 cttggaccgc caacccacga ccgaggcgtc cggtctctcc agaccggcgt ccaccgcgtc
     1681 gagcatgagg tcgagaagct cggccttggc cggcacgtac cggtagagcg acatcgtccc
     1741 taccccgagg tgctccgcga cccgtctcat ggacagcgct ctgagaccct ctgcgtcggc
     1801 cacctcgatc gccgcagcca cgatctgatc gaggctcaga cccggtttgg ggccgcggct
     1861 cggcttgatc tcgaggcgcc agaggagctc caggctccgc ttcggatcgc tccgtccgct
     1921 gtagttgtat tcgctcgtca tgggttgacc catcctagaa ctgcgtatag cgtacgccca
     1981 gcgacgtaca gtgtacccag ttaacatcat aagagccaca ggaggtcggg aatgggcgga
     2041 gatttcgcag tcgtagccga ggggctgcgc aagagttatg ggaagacctt ggctctcgac
     2101 gggttcgacc tcgcggtccc gtcagggagc gtttgcgggt tgctcgggcc caacggagcc
     2161 ggcaagacca ctgccgtccg gatactcggc accctgcttc gcttcgacga cggccgtgcg
     2221 gaggtggggg gcatcgacgt caggcgcgat ccggctcagg tgcgttctcg aatagggctt
     2281 acgggacaga acccggcggt cgacgagatc ctcacggggc gccagaacct cgtgatgttc
     2341 ggcaagctga accacctcga tgcagagtcg gctcgacggc gcgcggacga actgttggag
     2401 caattcggac tggccgacgc tgccggcaag caggcgaaga gctactccgg cggaatgaag
     2461 cggcggctcg acctggctgc cagcttcatc cttgcgccac aggtgatgtt cctggacgag
     2521 ccgacgaccg ggcaggaccc gcgcaaccgc aacgaagtct ggaacgtcgt acgcgagctg
     2581 gtggccggag ggacgacggt gttgctgacc actcactacc tcgaggaggc ggatcaactc
     2641 gccgatcaga tctccgtgat cgatcgcggc cgagtgatcg cagacggcac gccggaacaa
     2701 ctcaagtcca agatcggcgg aagcagggtc gacatcgtct taaacaacgt cgacgagtta
     2761 ccgatcgtgg ccgggctcgt cgaacgcgtt tgcggcggtg aagccgaagt cgatcacgac
     2821 aggcggcgcg taagtgcgcc ggtgaaagat cgagtcgcga cccttaccga ggtcctgcgt
     2881 gcgctcgagg acgccggcat cctggctgag gacgtcgcgg ttcgcaaacc cacactggac
     2941 gaggtgttcc tcgagctcac cggacaccgc gctgacgagg aggtggcggc atgacgccgg
     3001 caagcgccct gcatccgcag acctacaccg cgcgcctgaa gtgggcggta aaggacggct
     3061 ggacagttgc ccaccgcgac ctgtcgcatt gggcacatca gccgggacag atcgtcgccg
     3121 gtttgatctt cccgatcgtg ttcatcctgc tcttcggcta tgtgtttggc agcggaatga
     3181 tcccgcctga gggcggcgat tacatcgact tcctgatggc gggtctcttc gcgcagacca
     3241 tggccttcgg aatcggcgag acgatggtcg cggtgaacac cgacgccgac aagggaatca
     3301 cggaccggtt tagatccctg ccgatggcgc agtccgcggt cgtcgtaggc aggaataccg
     3361 ccgatatgtt gaactccgcg accggactga tcgtgatgat cgcttgcggt ctcgtggtgg
     3421 gttggaggtg gcacgagggg atcggcaacg cgctgcttgc cgtcggcctg ctgctgttgc
     3481 ttcgcttcgc attcttgtgg ataggcatct acatcggcct cgtcgtgaaa tctcccgagg
     3541 cactcggggc ggtgtggggg ctgctgttcc cggtgacgat gctcacgaac gcgttcgtcg
     3601 atcctcggtt catgcccgat tggctcggca cgattgcgga atggaacccg atctcgtcca
     3661 cgatttcggc tacgcgcgat ctcttcggta acgcaggtgc ctccgccgga ggaacttgga
     3721 tcgcagagaa cgcgatgatg atggctgtcg tgtggccgct gctgatagtc gtggtcacac
     3781 tccccctggc cgtgcgccgc tatcagaacc tcagtcgttg aagctctaac cggctaagga
     3841 gcgcgcgtcc tcaaggctcg gggcgattcc gattttcacc tcgtcgccgg gacacgtgat
     3901 gaccgcgtcc tgctcgccac cctcggcaac gagggcggcg tcaccctcgg tgctctcgtc
     3961 atcgtccgag aacgaggctg agtacc
//
LOCUS       DATMED010000079         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4669424, whole genome shotgun sequence.
ACCESSION   DATMED010000079 DATMED010000000
VERSION     DATMED010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4669424"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VNP73_01990"
     CDS             complement(<1..663)
                     /locus_tag="VNP73_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWL64718.1"
                     /translation="MDRRGFLKRAGLASAVGGAAVLKGRDLVRAQGQHAIHTMKHGGH
                     GAVGEVDHARNGFDPTDMLTDFDGGEVSKDRSGRTVREYEFLAVAKDIEVAPGIFFPA
                     WTYNGRVPGPTIRATEGDRIRIKFVNSTEHPHTMHFHGIHPAAMDGVPGQGEAKSGEE
                     FVYDFIAEPFGTHHYHCHSVPLAQHIHRGLYGAFIVDPKKGRPDANEMVVVMNAFDTN
                     FDG"
     gene            complement(656..1360)
                     /locus_tag="VNP73_01995"
     CDS             complement(656..1360)
                     /locus_tag="VNP73_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWL64719.1"
                     /translation="MLQHTSPAVQDYLKALHMLEWEGDLPAQGRTVSTQSLAQRLDVS
                     AASATNMLKKLDGLGLVEHVPYKGASLTDDGRKIALEVVRHHRLLETYLAEVIGLPWD
                     EVHAEAEILEHVLSEKLEDRISTLLGDPAADPHGHPIPAKDGSMPRFSSDRLWDIPDG
                     RRTTVERVADEDPAALRYLAEAGIRPGAEIDVLRRGPVDGPLFVRVPGGGDPNGETAL
                     SRELAASVWVQGGRDG"
     gene            1448..>2282
                     /locus_tag="VNP73_02000"
     CDS             1448..>2282
                     /locus_tag="VNP73_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWL64720.1"
                     /translation="MSHTETRAGRVERAALVAVLVTLVLAVAKVAVWAATDSLAVLSQ
                     ALDSALDLISLGLLFVGVRIAGKPADHTHHYGHSKAENLVAFTQTLFLCVIALVVAGA
                     ALARLSDAPIRVTTPWYAFVLLAASAAVDGIRVKWLYSAARAERSDALLSGALNLFLD
                     VGTAIVAMISLLLVRSGILEADAYGGLLVAGIVLASAIRVGKRSVDILMDRAPQALDE
                     AIEAAASRIPGVSETRRVRVRGTGDQLFADVTVAAGRTASLERAHDIAEAVEREIRDI
                     AP"
BASE COUNT          366 a          775 c          724 g          417 t
ORIGIN      
        1 accatcgaag ttcgtgtcga acgcgttcat cacgaccacc atctcgttcg catccggtcg
       61 ccctttcttg ggatccacga tgaaagcgcc gtagagaccg cgatggatgt gctgagctag
      121 gggaaccgaa tggcagtgat agtggtgcgt cccgaatggt tcagctatga agtcgtagac
      181 gaactcctca ccgctcttcg cctcaccttg cccgggaaca ccgtccatgg ccgcggggtg
      241 gatcccgtgg aagtgcatcg tgtgcggatg ctcggtggaa ttgacgaact tgatcctgat
      301 gcggtcgccc tccgttgccc ggatcgtcgg tccggggacg cggccgttgt aggtccacgc
      361 gggaaagaag atgccggggg ccacctcgat gtccttggca acggcgagga actcgtattc
      421 ccgcacggtc ctgccggagc gatctttcga gacttctcca ccatcgaagt cggtaagcat
      481 gtccgtcgga tcgaacccgt tgcgagcgtg atcgacctcg ccgaccgcgc cgtgaccgcc
      541 gtgtttcatc gtgtggatcg cgtgttgacc ttgcgcccgt accagatcac gacccttgag
      601 aaccgcggca ccgcccaccg cgctcgcaag cccggcccgt ttcaagaaac cccttctatc
      661 catctctgcc tccctgcacc caaacgctcg cggccagctc gcgcgacagc gccgtctctc
      721 cgttgggatc ccctccaccc ggcacgcgca cgaacagcgg gccgtcgacc ggccccctgc
      781 gcaacacatc gatctctgca cccggacgga tcccggcctc ggcgaggtag cgcaacgccg
      841 cgggatcctc gtcggctacg cgctcgacgg tcgtcctgcg cccgtcgggg atgtcccaca
      901 accgatcgct cgagaagcgc ggcatcgagc cgtctttggc ggggatcgga tgaccgtgcg
      961 ggtccgcggc cggatcgccg agaagggtcg agatgcggtc ctccagcttc tcggagagga
     1021 cgtgctcgag gatctcggcc tcggcatgga cctcgtccca cggcagcccg atgacctccg
     1081 ctagataggt ctctagaaga cggtggtgcc gcacgacttc gagcgcgatc ttgcgcccgt
     1141 cgtcggtgag tgacgctccc ttgtagggca cgtgttcgac gagacccaaa ccgtccagct
     1201 tcttcagcat gttggtggcc gaggccgccg acacgtcgag acgctgggcc agcgactgag
     1261 tagagacggt gcggccctgg gccggaaggt cgccctccca ctcgagcatg tggagcgcct
     1321 tcaggtagtc ctggacggcg ggggatgtat gttgcaacat aaatctagat tagccgaggc
     1381 taaacctccc ggcaagctcg ctacgcgacg ggggcccctt cccccgcccc tctaacctgt
     1441 acgagcgatg tctcacaccg aaacccgggc cggccgggtc gagcgagcgg cgctggtggc
     1501 cgtcctggtc accctcgtgc tcgccgttgc caaggtcgcc gtgtgggcgg cgaccgacag
     1561 cctcgccgtc ctgtcgcagg cgctggactc ggccctcgac ctgatctcgc tggggcttct
     1621 gttcgtgggg gtccggatcg ctgggaagcc ggcggaccac acccaccact acggccactc
     1681 gaaggccgag aacctcgtgg ctttcaccca gaccttgttc ctgtgcgtga tcgcactcgt
     1741 ggttgcgggg gccgcgctcg cccgtctgag cgacgcgccc atccgggtca ccacgccctg
     1801 gtacgcgttc gtcctgctgg cggcctcggc cgcggtcgac gggatccggg tcaagtggct
     1861 ctactcggcc gcccgcgccg aacgttcgga tgccttgttg tcgggtgcgc tcaacctctt
     1921 cctcgatgtt ggtaccgcca tcgttgcaat gatcagcctg ctgctcgtgc gaagcggcat
     1981 cctcgaagcc gacgcttatg gaggattgct ggtcgcgggg atcgttctcg cctccgcgat
     2041 ccgggtcggc aagcgctcgg tcgacatcct gatggatcga gccccccaag ccctcgacga
     2101 agcgatcgag gctgcggcca gtcggatccc gggcgtttcc gagacgagac gcgtccgagt
     2161 ccgcggcacc ggcgaccaac tattcgcgga cgtcaccgtt gcggcgggac gcactgcttc
     2221 tctggagcga gcccacgaca tcgccgaagc cgtcgagaga gagatcagag atatcgcgcc
     2281 gg
//
LOCUS       DATMED010000080         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_66576, whole genome shotgun sequence.
ACCESSION   DATMED010000080 DATMED010000000
VERSION     DATMED010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66576"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VNP73_02005"
     CDS             complement(<1..1037)
                     /locus_tag="VNP73_02005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL64721.1"
                     /translation="MKIAIVCPYAWDRPGGVQSHVKALGSALKDRDHDVLTIAPVCSR
                     DATVSEEITAIGRAVGIPANGSVAPIAFGPLATIGVRRALAAFEPDVVHLHEPLIPSV
                     SLLALWNAQAPCVGTFHAAAEQSAGYRVARTALDRAARKLAIRTAVSDAARALVARYF
                     PGDYLITPNGVDVERFANAEPLDLGPGRKVLFFGRLERRKGVEVLIQALTRLRDLDLE
                     LIVGGTGPEEGNARVLARQLNVRARFLGPVDDDDVPRLYKAADVYVAPGLGGESFGIV
                     LLEAMAAGTPVVCSSIAGFRGAAAQAAAFVPPDQPGPLADALRDVLGGWGEELAKKGR
                     MRASSFDWSRLV"
     gene            complement(1034..1957)
                     /locus_tag="VNP73_02010"
     CDS             complement(1034..1957)
                     /locus_tag="VNP73_02010"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HWL64722.1"
                     /translation="MTSRPRPPDESRLLQLVYYGYVAASAVARALPEKAAYRTAYYLG
                     SVAAKRSKQRHQVARNLARVTGYSVGSREIDELVEEAFRSYARYWFETFRLVRADKEF
                     FLERTVTVGGDKLIKAAASGGGIAVVSHLGNYDAAGAWAGAEGVKVVTVAEVLRPRRM
                     FDFFVKHRSRLGMTIHPAQPGVTDRLAAAARDDAIVAIVGDRDLKGTGEEVEFFGEPA
                     TFPRGPAVVALKSGAPLMVVGVYNTQLEDGKPGWVVDVGDPLDIPTEHTEENVAAITR
                     MVARELEGFVARSPADWHVFQPFWKADRAGR"
     gene            complement(1954..>2172)
                     /locus_tag="VNP73_02015"
     CDS             complement(1954..>2172)
                     /locus_tag="VNP73_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64723.1"
                     /translation="LVFSFLVSYARARAESLGFDCKVGIAERAERVIVMVIGLVFDIV
                     PIAIVLLSVASAVTFVQRVLHVRAQARR"
BASE COUNT          382 a          759 c          692 g          339 t
ORIGIN      
        1 acgagtcgcg accaatcgaa ggaggacgcc cgcatccggc ccttcttcgc cagctcctcg
       61 ccccatccgc ccaacacgtc gcggagcgcg tcggccagtg ggcccggctg atccggggga
      121 acgaacgctg ccgcctgcgc cgccgcgccc cggaagcccg ctatcgatga gcagacaacc
      181 ggggtccctg ccgccatcgc ttcgagcaga acgatcccga acgactcccc accgagaccg
      241 ggagcgacgt acacgtcggc cgctttgtac aggcggggaa catcgtcgtc gtcgacgggg
      301 ccgaggaaac gagcgcgcac gttcagctga cgcgcaagca cccgtgcgtt cccttcctcc
      361 ggacccgtcc ctccgacgat cagctcgaga tcgaggtcac gtaggcgagt gagcgcctgg
      421 atcagaacct cgaccccctt ccgtcgctcg agccggccga agaagagcac cttgcgaccg
      481 ggcccgagat cgagtggctc cgcattcgcg aagcgctcga catcgacccc gttcggcgtg
      541 atcaggtagt cgcccgggaa atagcgggcg acgagggcac gagccgcgtc cgaaaccgca
      601 gtcctgatgg cgagcttccg cgctgcccga tcgagagccg ttcgagctac ccggtaaccc
      661 gcgctctgct cggccgcggc atggaacgtg cccacgcagg gggcttgcgc gttccagagc
      721 gcgagtagag aaacgctagg gatcagcggt tcgtggagat ggacgacatc tggctcgaac
      781 gccgccaggg cgcgcctaac gccgatcgtc gcgagggggc caaacgctat cggagccacc
      841 gatccgttcg ccgggatgcc gactgcgcgc ccgatcgcgg tgatctcttc cgacactgtt
      901 gcgtctcggc tgcacaccgg agcgatcgtt agcacgtcgt ggtcgcggtc cttcagcgcc
      961 gagccgagtg ccttgacgtg actctgcacc cctcccgggc ggtcccacgc gtagggacac
     1021 acgatcgcga tcttcatcgg ccggcccgat ccgctttcca gaacggttgg aacacgtgcc
     1081 agtcggccgg gctgcgagcc acgaaccctt ccagttcccg agcgaccatg cgcgtgatgg
     1141 ccgcgacgtt ctcttcggtg tgctcggtcg ggatgtcgag cggatccccg acatcgacca
     1201 cccatccagg cttcccgtcc tccagctgcg tgttgtagac gccgacgacc atcagcggag
     1261 ccccggactt gagggccacg accgcgggcc cccgggggaa tgtcgccggc tcgccgaaga
     1321 actcgacctc ttcacccgtt cccttcaggt cgcggtcgcc gacgatcgcg acgatcgcgt
     1381 cgtcgcgggc agctgccgca agtcgatcgg tgacaccagg ctgggccgga tggatcgtca
     1441 tccctagtcg ggaccgatgc ttgacgaaga agtcgaacat cctgcgggga cgtaacacct
     1501 cggccactgt cacgaccttt accccctcgg ctcccgccca tgcgccggcg gcgtcgtagt
     1561 tgcccaggtg gctcacgacg gcgatcccgc ccccggacgc agcagctttt atgagcttgt
     1621 cgccaccgac cgtgaccgtg cgttcgagga agaattcctt gtccgcccgc accagcctga
     1681 atgtctcgaa ccaatagcgg gcataggagc ggaacgcctc ctcgacgagt tcgtcgatct
     1741 cacgcgaccc gacggagtag ccggtcacac gggccaggtt gcgcgccacc tggtggcgtt
     1801 gcttggagcg cttcgcggcc accgagccca ggtagtacgc ggttcggtag gcagccttct
     1861 caggtagagc acgggccacg gccgaggcgg cgacgtaccc gtaatagacg agttgcagca
     1921 acctgctctc gtcgggcggt cgcggccgtg aagtcatctc ctagcttgcg cgcgcacgtg
     1981 gagaacccgc tgcacgaagg tgacggccga tgcgacgctc aacagcacga tggcgatcgg
     2041 gacgatgtcg aagaccagcc cgatgaccat cacgatgacg cgctcggccc tttccgcgat
     2101 cccgaccttg caatcgaagc cgaggctctc ggcccgagcc cgtgcgtagc tgacgaggaa
     2161 cgagaagacg ag
//
LOCUS       DATMED010000081         2992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3132634, whole genome shotgun sequence.
ACCESSION   DATMED010000081 DATMED010000000
VERSION     DATMED010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132634"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1418)
                     /gene="murC"
                     /locus_tag="VNP73_02020"
     CDS             complement(57..1418)
                     /gene="murC"
                     /locus_tag="VNP73_02020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWL64724.1"
                     /translation="MIGRSGRVHFVGMGGVGMSAIAKVLIEQGYEVSGSDLKRSRALT
                     TLEAMGATISLGHSEANVVGAGLVVVSSAIPDANPELRKARSLDLPVLGRGEALAELL
                     EASKSIVVAGTHGKTTTTSMIVSILRHAGMDPTYLVGGGLNDAGTNARYGRGEFSVAE
                     SDESDGSFLLLRPHIAVVTNIEADHLDYWSSLAEIRAAFQRFVGSSDPSGASVLPSVE
                     KDLVESAPGRSITFGDTGDVRAGNIVTQGEGARFELSIGDAVVPVVLKVPGKHNVDNA
                     AAAAAAAHAIGVSPEQIGMGLGTYRGVERRFQLRGTVNGVTVIDDYAHHPTEIRSTLA
                     AARSGGWQRLIAVFQPHRYSRTAAFAKEFGSSFSDADVVVVTDVYGAGERAVPGVTGK
                     LVADAVCTNLRGRTVAYFPHRDEVIGYVRAQARPGDAVLTLGAGDVTLLGEELLMRWS
                     SDG"
     gene            complement(1415..2494)
                     /gene="murG"
                     /locus_tag="VNP73_02025"
     CDS             complement(1415..2494)
                     /gene="murG"
                     /locus_tag="VNP73_02025"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWL64725.1"
                     /translation="MKVVIAGGGTAGHVNPAIALAEALEGADISFVGTARGVEAEAVP
                     RAGLPLETIEVVGFDRARPWSFPGVGWRAVRATRSAATLLRSLKPDCVVGMGGYVSLP
                     VGLAARRLRLPLVVHEQNIVLGLANKVLKRFARVVGVSFEETLAQVGPRGRYVGNPVG
                     SALASCDWAVARERGLETFGLDPSRRTLLVFGGSLGAKTINDAARGLSSLWAGRSDRQ
                     VVHISGSRHEPVATEAIGGLLWRSVGYVEGMEEAYAVADLALCRGGATTVAEIAVAAL
                     PAMIVPYPYHRDRQQERHATVFAREGAAVVLADRDTTAEEVARVADDLLSDPVTLKAM
                     GDSARSLARPSAAKDLADLVRAAAA"
     gene            complement(2491..>2992)
                     /locus_tag="VNP73_02030"
     CDS             complement(2491..>2992)
                     /locus_tag="VNP73_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWL64726.1"
                     /translation="AARSTSAMGSSMIPLYTGYQTIQSLIALGSGGWFGVGLGASRQK
                     WLYIPNAHTDFIYAILGEEMGLLGTLTVLGIFAFVAFLGIKIARRAPDRFGMLLASGI
                     TVWIGFQALVNIGAVTASMPITGIPLPFVSFGGSSLVISLIAIGILSNIALNSKPPRS
                     RSRSDR"
BASE COUNT          579 a         1050 c          877 g          486 t
ORIGIN      
        1 gttcgagccg tccctcgaag ccgagctcgt taacgctcgc ctccaaggcc acgatctcat
       61 ccatccgagg accaccgcat caggagttct tcgccgagga gggtgacgtc cccggccccc
      121 agcgtgagta ccgcatcacc aggccgcgcc tgcgccctta cgtagccgat cacttcgtcc
      181 ctgtggggga aataggcgac cgttcgtccg cgcagattcg tgcacacggc atcggccacg
      241 agcttgcccg tgaccccggg aacagctcgc tcccccgctc catacacatc cgtaacgacc
      301 acgacatcgg cgtcggagaa cgaagaaccg aattcctttg cgaatgcggc cgtgcgcgag
      361 tatcgatgcg gttggaacac cgcgatcaag cgctgccagc ccccgctgcg tgcggcggcc
      421 aatgtggacc tgatctcggt cgggtgatgc gcgtagtcgt cgatcaccgt gacaccgttc
      481 accgtgcccc tcaattggaa gcgacgttcg acaccgcggt aggtaccaag tcccatgccg
      541 atctgttctg gactcacccc gatcgcgtgt gctgctgccg cagcggcggc agcattgtcg
      601 acgttgtgct tgcccggaac cttgagcact acgggaacaa ccgcgtcgcc gatcgacagc
      661 tcgaaacgtg caccctcacc ttgggtcacg atgtttccgg ccctgacatc tccggtgtcg
      721 ccgaacgtta tcgagcgacc cggggccgac tcgaccaggt ctttttcgac agagggcaac
      781 acactggcgc ccgaggggtc cgaagagccg acgaagcgct ggaacgccgc tcggatctcg
      841 gccagtgagg accagtaatc gagatgatcg gcttcgatgt tcgtcaccac cgcgatgtgc
      901 gggcgcagca gcaagaagga cccgtcgctc tcgtcggact cggctaccga gaactccccc
      961 ctcccgtacc gggcattcgt tccggcgtcg ttgagaccgc cgccgaccag ataggtgggg
     1021 tccatccccg cgtggcgcag gatggacacg atcatcgagg tcgtcgtcgt cttgccgtgt
     1081 gtccccgcca ccacgatcga cttagacgcc tccagcagct cggccagggc ctcgccgcga
     1141 ccgagcacgg gaagatcgag agatcgagcc ttgcgcagct ccggattagc gtccgggatg
     1201 gccgatgaca ccaccacgag tccggcgccg actacgttcg cttcgctgtg ccccaggctg
     1261 atcgttgccc ccatcgcctc cagcgtggtc agcgcccgcg accgtttcag gtccgatccg
     1321 gatacttcgt atccctgctc gataagaacc ttcgcgatgg cggacatgcc gacgccaccc
     1381 atccccacga aatggacgcg gccggagcga ccgatcacgc ggcggccgcc cgaaccagat
     1441 cggccaggtc cttcgcagcc gagggacgtg caagagagcg cgctgaatca cccatcgcct
     1501 tcagtgtgac gggatcggac agcagatcgt cggcaacgcg cgccacttct tctgctgttg
     1561 tgtcccggtc tgcaagcact acggccgccc cttctcgtgc gaacaccgtg gcgtgacgct
     1621 cctgctgtcg gtcacggtga tagggatagg gaacgatcat cgcgggtaga gccgcgaccg
     1681 cgatctcggc caccgtggtt gctccgcctc tgcacagagc gagatcggcg accgcgtaag
     1741 cctcttccat accttcgacg taaccgacac ttctccacag cagccccccg atcgcttcgg
     1801 tggctaccgg ctcgtgcctc gatcccgaga tgtggacgac ctgtcgatcg gagcgacccg
     1861 cccataagga gctcagccca cgtgccgcgt cgttgatcgt cttcgcgccg agactcccgc
     1921 cgaagacaag cagtgtgcgc cgggatggat ccaaaccgaa ggtctccagt ccccgctcac
     1981 gggcaacggc ccagtcgcag cttgccaatg ccgacccgac gggattaccc acgtaacgac
     2041 cgcgcggtcc cacctgcgcg agcgtctcct cgaacgaaac gccgacgacg cgcgcgaacc
     2101 tcttcaggac cttgttagca agaccgagaa cgatgttctg ttcgtgcacg accagaggaa
     2161 gtcgcagccg cctagccgca agccctaccg gcaggctgac gtaaccaccc atccccacca
     2221 cgcaatccgg cttgagcgaa cgcaacaagg tcgccgcaga acgcgtagcg cgcacggccc
     2281 tccagccaac gcccggaaac gaccacggcc gcgcgcgatc gaaccccact acttcgatcg
     2341 tctcgagcgg caatccggct cggggcacgg cctccgcctc gaccccccgg gccgtcccca
     2401 cgaaggagat gtcggctccc tcgagcgctt ccgccagcgc tatcgccgga ttgacgtgac
     2461 ccgcggtgcc accacccgcg atcacgactt tcatctatcg gaacgagacc gcgatctcgg
     2521 aggcttcgag ttgagtgcga tgttcgacag gatcccgatc gctatcaggg agatgaccag
     2581 ggaacttccg ccgaaagaaa cgaacggcaa cgggatgccg gtgatcggca tcgatgccgt
     2641 caccgcaccg atgttcacca acgcctggaa accgatccac accgtgatgc cggatgccag
     2701 cagcatgccg aaccggtccg gcgcacggcg ggcgatcttg atccccagga acgccacgaa
     2761 ggcgaagatc ccgagcacgg tcaacgtgcc gaggagaccc atctcctccc cgaggatcgc
     2821 gtagatgaag tcggtgtgcg cgttcgggat gtaaagccac ttctgccggc tggccccgag
     2881 gccaactccg aaccagccac ccgatcccag cgcgatcaga gactgaatcg tctgataacc
     2941 ggtgtacaac gggatcatgg acgagcccat ggccgatgtt gaacgcgcag ca
//
LOCUS       DATMED010000082         5783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1205257, whole genome shotgun sequence.
ACCESSION   DATMED010000082 DATMED010000000
VERSION     DATMED010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205257"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VNP73_02035"
     CDS             <1..667
                     /locus_tag="VNP73_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL64727.1"
                     /translation="GRFVQSAIDHFGKLDVLVNNAGILRDRMIVNMTPEEWDAVIHVH
                     LRGTFCCTQHASAHWRKRSKDGEELDVRIINTSSPSGLYGNIGQSNYGAAKAGIASFT
                     IIVARELERYGVTVNAISPAARTRMTENLGIGSVEIAEGEFDPFGPENISPIVVWLGS
                     AQSKGITARVFDVRGGRIGIAEGWHRGPHVEKDARWEPEAVGPVIEELLARAAPIANM
                     REG"
     gene            668..1513
                     /locus_tag="VNP73_02040"
     CDS             668..1513
                     /locus_tag="VNP73_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWL64728.1"
                     /translation="MPIDPAAVGTEYGPFERTWESKDTLLYALGVGCGTDELQFTTEN
                     SIDIDQQVLPTFATVAAGGAGGGLSSIGDINPAMLVHGEQAIELHSALPVEGTVSTSG
                     KVAHIWDKGSGAVVVAESLTTDKRTGDKLFSSRTTIFIRGEGGFGGDRGPSGPRNVPP
                     DRAPDHVVSYETTPEQALLYRLSGDRNPLHSDPKFAAMGGFDKPILHGLCTYGFTGRA
                     LLHSLCGSDPARFRGMEARFSKPTWPGDTLTIRMWVDGNEAIFTTQTQRDDTVIEQGL
                     FTFDG"
     gene            1529..2632
                     /locus_tag="VNP73_02045"
     CDS             1529..2632
                     /locus_tag="VNP73_02045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64729.1"
                     /translation="MTQSGSAGVFTQEQEELRESARRLLAEKSGSADIRRLAEGGESF
                     DPDTWKQIAELGWLGIAIPEEYGGLGYSFVELFVILEEMGRRLLVAPFFSSIVLAAPA
                     IELAGNDQQKKNLLAPIADGSLRATLAATSGTDARATQANEGYALDGNVSFVLDGADA
                     DTIIVAAASDGGTSLFVVESNASGLERVPLTTLDETRRFAELRFSSTPAELLGEAGNG
                     DSVLTQVLDRAAIALAGEAIGGAQECLEQTVQYAKDRTQFDRPVGSFQAIQHRLADLY
                     MEVEMARSAAYYAAWAAAENSDEVPRVAPLAKAYCTETFFHAASESIQVHAGIGFTWE
                     HDAHLYFKRAQSSALFLGTAAENRERLAQRLEI"
     gene            2632..3648
                     /locus_tag="VNP73_02050"
     CDS             2632..3648
                     /locus_tag="VNP73_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWL64730.1"
                     /translation="MRLGLNLGYWGSIPVDQVALAQAADKLGFHSVWTAEAYGSDVLT
                     PLAWVAAKTENINVGTAIMQMPARQPTAAAMAAATLDQLTGGRMLIGIGASGPQVSEG
                     WYGVPYGKPLKRTREYVHIIREVLKREGPFEFHGEYYDIPVKGGTGLGKPLKLITKPL
                     RSDIPIYLAAIGPKNVALAAEIAEGWLPVFYSPYRTEIHQESLENGFAKSGKNPDAFD
                     IAASCVVIMGDDIDGCRSLVKPHLALYIGGMGARGKNFYNDLACRMGYEAAAKEIQDL
                     YLDGKKDEATAAVPDEFVDEVTLCGPKERIIERLEAWKSSNVTTLVVSTIQTEVLDIL
                     AEAL"
     gene            3666..5177
                     /locus_tag="VNP73_02055"
     CDS             3666..5177
                     /locus_tag="VNP73_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HWL64731.1"
                     /translation="MANTVVETTYGKLDGLDKGDVLQFRGIPYAAPPVGDRRFRPPAP
                     PDAWTDVREAKTFGPAAPQSSSAIDLLNTRELEMSEDCLTLNVYTPGADDARRPVMVW
                     IHGGAFVTGTGSSPWYNGTTFASKGDVVVVTINYRLGALGFMYLDDIVEDIQDSANLG
                     ILDQIAALEWVRDNIAAFGGDPENVTVFGESAGAMSIGTLLGTPAAKDLFHRAVLQSG
                     AVRHMSDRDEASRIAKDLTGHLGLDRLSAATLREIQFEQLIEAQGITFMSYWGKTRGL
                     PLQPVLDGVVLPEHPLQAIENGSTAGKELLAGTTRDEMLLFALLDPKHQTLTEEELVK
                     RATRAFGDAAGATRAIATYRGNRPDATPGEISSAIQTDYVFRSSMNELVDKHQGDAFV
                     YLFEYCSPVMGGKLGSCHALEIPFVFNALDAPGADQFSGPPTAEMRELAFAMNEAWTN
                     FARSGRPQSTLLPEWPPFDDQRRTMVFDIESRVIEDPLGNERLLWLGAARPSR"
BASE COUNT         1172 a         1782 c         1827 g         1002 t
ORIGIN      
        1 cggccgtttc gtccagagtg cgatcgacca tttcggcaag ctcgacgtgc tggtgaacaa
       61 cgccggcatc ctgcgcgacc ggatgatcgt gaacatgacc cccgaggaat gggacgcggt
      121 gatccacgtc catctgcgag ggacgttctg ctgcacccaa cacgcgtccg cgcactggcg
      181 caaacggtcg aaggatggcg aagagctcga cgttcgcatc atcaacacga gctcgccgtc
      241 gggcctctat ggcaacatcg gacagagcaa ctacggcgcg gccaaggcgg ggatcgcctc
      301 gttcacgatc atcgtcgccc gggagctcga acgttacggc gtgacggtca atgcgatctc
      361 cccggcggcg cgcactcgga tgaccgagaa cctggggatc gggtctgtcg agatcgccga
      421 gggtgagttc gacccgttcg gcccagagaa catctcaccg atcgtcgtgt ggctgggcag
      481 tgcgcaatcg aaagggatca ccgcacgcgt attcgacgta cggggaggtc gcatcgggat
      541 cgccgagggc tggcaccgcg gcccccacgt cgagaaggac gctcgctggg aaccagaagc
      601 agtcggaccg gtgatcgagg aattgctcgc gcgggcagcc ccgatcgcga acatgaggga
      661 ggggtagatg ccgatagatc ctgccgcggt tggaaccgag tacgggccgt tcgagcggac
      721 gtgggagtcc aaagacacgt tgttgtacgc gctcggcgtc ggctgcggca ccgacgagct
      781 ccagttcacg actgagaact cgatcgatat cgatcaacag gtgctgccga ccttcgcaac
      841 ggttgccgcg ggaggcgcag gaggcggcct gagttcgatc ggggacatca atcccgcgat
      901 gctcgtgcac ggagagcagg cgatcgagct tcactccgca ctccccgtcg aggggaccgt
      961 ctcaaccagc ggcaaggtcg cgcatatctg ggacaagggg tccggggccg tcgtggttgc
     1021 cgaatccttg accacggaca agaggaccgg cgacaagctg ttctcgtccc ggacgacgat
     1081 attcatccgg ggagaggggg gcttcggcgg cgaccgcggg ccgtccggcc cccggaacgt
     1141 gcccccggac cgtgcaccgg accacgtcgt ctcgtacgag acgacgcccg agcaagctct
     1201 tctctaccga ctgtcgggag accgcaatcc tttgcactcg gatccgaagt tcgccgcgat
     1261 gggcgggttc gacaaaccga tcctccacgg actttgcacg tacgggttca ccggccgtgc
     1321 attgttgcat tcgttgtgtg gctcggatcc ggctcggttc agaggtatgg aggcgcgctt
     1381 ctcgaaaccg acgtggccgg gcgacaccct cacgatccgg atgtgggtcg acggcaacga
     1441 agcgatcttc acgacacaga cgcaacgaga cgacacggtg atcgagcaag gcttgttcac
     1501 cttcgacggc tgaggaaagg cgaagcagat gacacagagc ggatcggccg gggtcttcac
     1561 ccaagagcag gaagagctac gcgagtcggc tcgccggctg ctggccgaga agtccggctc
     1621 ggccgacata cgcagactcg cggaaggcgg cgagtctttc gaccccgata cgtggaagca
     1681 gatagccgag ctgggctggt tgggcatcgc cataccggag gagtacgggg gcctcggcta
     1741 ttcgttcgtc gagctattcg tgattctcga ggagatgggc agaaggttgc tcgtcgcgcc
     1801 gttcttctct tcgatcgtgc tggcggcgcc ggcgatcgag ctggcgggta acgatcagca
     1861 gaagaagaac cttctcgctc cgatcgcaga cgggtcgttg agggcaaccc tggcagcaac
     1921 ttccgggacc gatgcacgcg caacgcaggc gaacgagggc tacgcgctgg acggaaacgt
     1981 ttcgttcgta ctggatggag ccgatgccga cacgatcatc gtcgcggccg cttccgacgg
     2041 aggaacgtcg ctgttcgtcg tcgagagcaa cgcgtccggg ctcgagcgtg tgcctttgac
     2101 aacgctggac gagacacgtc ggttcgccga gcttcgtttc tcctcgacgc ccgccgagct
     2161 actgggtgag gccggcaacg gagactccgt cttgacacag gtgctggacc gcgccgccat
     2221 cgccttggcc ggggaagcga taggtggagc acaggagtgc ctcgagcaaa ccgtgcagta
     2281 cgccaaagat cggacccagt tcgaccgccc cgtcggctcc ttccaagcga tccagcaccg
     2341 gctcgcggac ctgtacatgg aagtcgagat ggcgcgatcg gcggcctatt acgcggcgtg
     2401 ggccgcggcg gagaactccg atgaggtgcc gagggtcgcc ccgctggcaa aggcctactg
     2461 caccgagacc ttcttccatg cggcgtcgga atcgatccag gtccacgcag gcatcgggtt
     2521 cacctgggag cacgacgctc acctctactt caagagggca caatcgtcgg cactgttcct
     2581 cgggacggcc gccgagaatc gcgagcgtct cgctcagaga ttggagatct gatgaggctc
     2641 ggactcaacc tcgggtattg ggggtcgatc cccgtcgatc aggtggcgct cgcgcaggct
     2701 gcggacaagc tgggcttcca ttcggtatgg actgcggagg catacggatc ggacgtcttg
     2761 acgcccctgg cgtgggtcgc ggccaagacc gagaacatca acgttggcac cgcgatcatg
     2821 cagatgccgg cccgccaacc gaccgcggct gcgatggcgg ctgcaaccct ggaccagctg
     2881 accggcggtc ggatgctgat cggtatcgga gcttccggcc cccaggtgtc cgaaggttgg
     2941 tacggggttc cttacggcaa accgctgaag cgaacgcgcg agtacgtcca catcatccgt
     3001 gaggttctca agcgcgaagg tcccttcgag ttccacggcg agtattacga catccctgta
     3061 aagggtggga ccggcctcgg caagcctctg aaactcatca cgaagccgtt gcgctccgac
     3121 atcccgatct atctcgccgc gatcggcccc aagaatgtcg ctctcgctgc agagatagcc
     3181 gaagggtggc tgccggtctt ctactccccc taccggaccg agatccatca ggaatcgctc
     3241 gagaacggct tcgcgaagtc gggcaagaac ccggacgcct tcgatatcgc agcgagttgc
     3301 gtcgtgatca tgggcgatga catcgacgga tgccgatcgc tggtcaaacc tcaccttgcg
     3361 ctctacatcg ggggcatggg agcgcgcggc aagaacttct ataacgacct cgcgtgcagg
     3421 atgggttacg aggctgccgc gaaagagatc caggatctct acctggacgg aaagaaggac
     3481 gaggccacag cggccgttcc cgacgagttc gtcgacgagg tcacactctg tggcccgaag
     3541 gaacgcatca tcgaaagact cgaagcgtgg aagagctcga acgtgacaac tctcgtcgta
     3601 tcgacgatcc agacagaagt cctagacata ctggccgaag cgctttaaca agggggcgaa
     3661 ccaacatggc gaacacggtc gtagagacca cttacgggaa gctcgatggc ctcgacaagg
     3721 gcgacgtgct gcagttccga gggatccctt acgccgcccc gcctgtcggc gatcgacgct
     3781 tcaggccccc ggcaccgccc gacgcttgga ccgacgtgcg ggaagcgaag acgttcggcc
     3841 cggccgcgcc tcagtcgtcg tcggcgatcg accttctgaa cacccgtgag ctcgagatga
     3901 gcgaggactg cttgacgctg aacgtgtaca cacccggagc ggacgatgcc cgtaggccgg
     3961 tgatggtgtg gatccacggt ggtgcgttcg tgacgggcac cggctcgagc ccttggtaca
     4021 acggcacgac cttcgcatcg aagggcgacg tggtcgtcgt cacgatcaac taccgcctcg
     4081 gtgcgctggg attcatgtac ctcgacgaca tcgtcgaaga catccaggac tctgcgaacc
     4141 tggggatcct cgatcagatc gccgccctcg agtgggtgcg cgacaacatc gcggcttttg
     4201 gaggcgaccc cgagaacgtg acggttttcg gcgaatctgc aggcgcgatg agcatcggaa
     4261 ccttgctcgg cacgcccgcg gcgaaggatc tgttccacag ggcggttctc cagagcggcg
     4321 ccgtcagaca catgagcgat cgggacgagg cttcgcgcat cgccaaggat ctaaccggtc
     4381 acctcggtct cgacaggctt tcggcggcaa cgttgaggga gatccagttc gaacagctga
     4441 tcgaagcgca ggggatcacg tttatgtcct attggggaaa gacgaggggg cttcctctcc
     4501 agcccgtcct cgatggcgtg gtcctgcccg agcatccttt gcaggcgatc gagaacggaa
     4561 gcacggccgg caaggaactc ctcgccggca ccacccgcga cgagatgctg ctgttcgcgc
     4621 tactcgatcc gaagcaccag acgctcaccg aggaagagct ggtgaaacgg gcgaccagag
     4681 cgttcggcga cgcggccggg gcgacccggg cgatagcgac ctaccgcggc aaccgacccg
     4741 acgcgacgcc gggcgagatc tcgtcggcga tccaaaccga ctacgtgttc cgctcatcca
     4801 tgaacgagct ggtcgacaaa caccagggag atgcgttcgt gtacctattc gagtactgct
     4861 cgccggtgat gggtggaaag ttaggttcat gccacgccct ggagatcccg ttcgtgttca
     4921 acgcgctcga tgcacccggc gcagaccagt tctccggccc gcctaccgcc gagatgcggg
     4981 agctcgcgtt cgctatgaac gaagcgtgga cgaacttcgc tcgcagcggc cggccccaat
     5041 ccactctgct gccggagtgg cccccatttg acgatcagcg tcggacgatg gtgttcgaca
     5101 tcgagagccg agtcattgaa gacccgcttg gcaacgaacg cctgctgtgg ctcggagcgg
     5161 cacgcccctc gagataatcc ttttcagaac gagatcaggg gccgtcgttg ctgctagaga
     5221 gaacgccagg cgctttgaag ccgttgccag gcaacgtaca tcccaacggc tgcgatcaga
     5281 aaattcacta ggcgacgaac ctcaagttcg ttcatccgaa tcccaccctt cgtctaaccg
     5341 tgcaccatcc atgagacaga catccgtttc cgccttattc ccaaactcca aaacttcagc
     5401 caaacgctga attgggggtt ccgccgcgtc gtcgaaaact gtcgttgcca tgaagtcagc
     5461 gatccacatt tgaaagccct cacggacctc cgcgtgttcc cgctgcactt caggggtagc
     5521 gggcaaatct ccgatggtct caatcacaga ccaaactaga agtgcgctgt cagaaactcg
     5581 catcgtgctc ctggcagaac cactaactgg ggcgcagttc acatcaatcc tcctctttga
     5641 gaaattgacc gagtagccgg catattcgac ctttgttccg actcaatcgg acacgggccg
     5701 cggaagcccg attgactgac catcccaaca gcctcgccac gtcctcctca gttgcattga
     5761 agaaagtctt gacaagacag aac
//
LOCUS       DATMED010000083         4600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5051204, whole genome shotgun sequence.
ACCESSION   DATMED010000083 DATMED010000000
VERSION     DATMED010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5051204"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..783
                     /locus_tag="VNP73_02060"
     CDS             100..783
                     /locus_tag="VNP73_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWL64732.1"
                     /translation="MLDIEPGTVVVWGDIGCPWAHLCVFRLHRARTEAGLDEEVTLDM
                     RAFPLEVFNRQPTPKKILDAEISVAGGLDPDAGWQMWQGAESEYPVTMLPALEAVQAA
                     KRQSLRASEELDRALRVALFAQSRTISMRHEILAVAKKCDRVDEAELGRALDTGSARS
                     EVMAQQKQAERDEVKGSPHDFLPDGSDVHNPGLELKWHGEHGEGFPVVVSDDPSVYKG
                     IVIRAAGST"
     gene            795..1088
                     /locus_tag="VNP73_02065"
     CDS             795..1088
                     /locus_tag="VNP73_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64733.1"
                     /translation="MDESNVRQKAQAHGAAMVAGDVNKAGGDLTQEAMAGAGAVMKEM
                     PRQIDSAEITDVAAAGDGFTVLIRYSGEGKDVTVKSVWIDKDGDPKIAELSLA"
     gene            1107..1496
                     /locus_tag="VNP73_02070"
     CDS             1107..1496
                     /locus_tag="VNP73_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64734.1"
                     /translation="MPEADVSGYQRKVLGSQVRPAKAVWGAEGIVIQDGRALPFVVER
                     QWNAPAGYYLEQWFLIDPGSREILHEGPAQQRLIWGLQSATEVGDRDPGGFPLPAGPC
                     RIVFALGGIIGGEADVHVAEATTESAA"
     gene            1739..2812
                     /locus_tag="VNP73_02075"
     CDS             1739..2812
                     /locus_tag="VNP73_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWL64735.1"
                     /translation="MRSRLVAALLVVALVGSSCAWAALSGLELNSTGTARIDPDFERD
                     VRPVAPSEVAEIVEKTLPSVVNVKVTSVAGRGEGSGVIIDEAGVVLTNAHVVEGSVSV
                     SVAFPDERPSVEGTVIGAIPLKDLAVIKIPPDDLDPVVIGSSENLRLGDDVIAIGYPL
                     GLGGPTVTKGIISGRARSITTDTSSGEEVNLDGLLQTDASINPGNSGGALLDSAGRLI
                     GINTAVAGFAENIGFAIAIDDALPTIEEILSEPPSQHAWLGVQSDRLDGNTANELGLD
                     PEVEGVVVVNVFPETPAEEAGIEEGDVITSVGGQTITSPVDLSDAITERDPGETVEIE
                     LVTPDGRRTVTARLARRPVTLEP"
     gene            2914..3108
                     /locus_tag="VNP73_02080"
     CDS             2914..3108
                     /locus_tag="VNP73_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWL64736.1"
                     /translation="MKVKLRNPDRLVDVTGPKNVRDLLRELDIVPEAVLVIRDATLLT
                     RDEGLADSDELEIRPVLSGG"
     gene            3112..4116
                     /locus_tag="VNP73_02085"
     CDS             3112..4116
                     /locus_tag="VNP73_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL64737.1"
                     /translation="MKCKVCREHAVVDVRRHNAAFCGDHFVAHIHSQVERTIKEFKMF
                     TPEDRLLIAVSGGKDSLAVWDVLVDLGYDATGFYLDLGIGGYSERSKEAAVAFAGARN
                     QKLIIRSLAEEHGFTVPELSRLTGRVPCSGCGLNKRYEFNRAALEEGFDILVTGHNLD
                     DEVATLFGNVLHWNTDMLGRQAPVLEERFLEGGPSDAEQLGARRVLVRKVKPLVRIAE
                     RESAAYALIRGIDYIIEECPMVEGNTQHRYKEAITLLEESSPGTKQHMYFGFLKRAAD
                     RFAPDAAASEIVACVDCGAPTIVWEEGQTPRCSFCKTKALALHRKETGERPAPRRRRR
                     "
     gene            4116..>4600
                     /locus_tag="VNP73_02090"
     CDS             4116..>4600
                     /locus_tag="VNP73_02090"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWL64738.1"
                     /translation="MESEGVKEEERPLQAGDRVLLIDSRDRRYMITLATGKQFHSHMG
                     SIEHNDLIGGPEGSVVTTTGGSKVLAFRPTLGDYTLKMKRGAQVVYPKDVGLILVYAD
                     VFPGAFVVEAGTGSGSLTLALARAVGESGRVISYEIREDHLDRAVENVGAWFEAAGGK
                     P"
BASE COUNT          847 a         1371 c         1602 g          780 t
ORIGIN      
        1 ggccctcgac gatgtcgcag aagatgcaca ccgggcgacc gtacccaatt gcccaaggcc
       61 cccgattgag ccgccggcgt gctggggaac atgtccctca tgttggatat cgaaccgggg
      121 acggtcgtcg tgtggggaga catcggatgc ccgtgggccc acctatgcgt cttccgactg
      181 caccgggccc gcaccgaagc cggtctggat gaagaggtca cgttagacat gcgcgccttc
      241 ccgctcgagg tgttcaatcg ccaaccgacg cccaagaaga tcctggatgc ggagatatcg
      301 gtcgcaggag gattggaccc cgacgcgggg tggcagatgt ggcagggggc cgagagtgag
      361 tacccggtca cgatgcttcc ggcactcgaa gcggtgcagg ccgctaagcg tcagagcctc
      421 cgagcctctg aagagctgga ccgtgcctta cgcgtagcgt tgttcgcgca gagtcgcacg
      481 atctcgatgc gccacgagat cctggctgtt gcgaaaaagt gcgatcgcgt ggacgaggct
      541 gagctcggca gagccctcga cacgggcagt gcgcggagtg aggtcatggc gcagcagaag
      601 caagcagagc gggacgaggt gaagggcagc ccccacgatt tcctccccga cggatcggac
      661 gtgcataatc caggtctcga gctgaaatgg cacggggagc acggagaagg attcccggtc
      721 gtggtgagtg acgacccttc cgtttacaag ggcatcgtca tcagagcggc cggctcaacc
      781 tagggggtgg cgggatggac gagagcaacg ttcgtcagaa ggcgcaagcg cacggtgccg
      841 cgatggtggc cggggacgtc aataaggccg gaggggatct cactcaagag gcgatggcgg
      901 gtgccggcgc ggtcatgaag gagatgcctc ggcagatcga cagcgccgag atcacggacg
      961 tggcggcagc gggagacgga ttcacggtgc tcatccgcta ttcgggagag ggcaaggacg
     1021 taaccgttaa gtcggtatgg atcgacaagg acggggatcc gaagatcgcc gaactctcgc
     1081 tggcgtgagc ttgctaccct ctcgccatgc ctgaagcaga cgtcagtggt taccagcgga
     1141 aggttctggg ttcccaagtc cgcccggcca aggccgtgtg gggtgctgag gggatcgtga
     1201 tccaggacgg ccgggctctg cccttcgtgg tcgaacggca gtggaacgct ccggccggct
     1261 actacctcga gcaatggttc ctgatcgacc ccggatcgcg cgagatcctc catgagggcc
     1321 ccgcccaaca acgcctgatc tggggtctgc agtcggcgac cgaggtcggg gaccgggacc
     1381 ccggagggtt ccctctaccg gcgggtccgt gccggatcgt gttcgctctg ggcgggatca
     1441 tcggcgggga ggcggacgtc cacgtggccg aagccaccac cgaaagcgcc gcctagcacc
     1501 ccggcgaaac gcgcggcggc ctcggccgtc gcgcagggct agcggcggcc tggggcgtcg
     1561 cggggggagg tattgacctg ccgggtcgcc tggggcacac tggtttggcc ggttactgag
     1621 gaacgcccga gcttccccta gggcgcccca caggagccgg ctctttcttt gcttgcgcga
     1681 cggccgaggc cgccgcgcgg cccgactaac gacacgcgct acttatcctt tcccggtcat
     1741 gcgttctcgg ttggtcgccg ccctgctcgt tgttgccttg gtgggctcgt cgtgcgcgtg
     1801 ggcggctctg tcggggcttg agctgaacag caccggcacc gcccggatcg atcccgactt
     1861 cgaacgagac gtgcggcccg tagccccgtc ggaggtggct gagatcgtcg agaagaccct
     1921 tccatccgtc gtgaacgtga aagtcacttc tgtcgccggt cgtggtgaag gctccggcgt
     1981 gatcatcgat gaggccggcg tggttctgac gaacgcgcac gtggtcgaag ggtcggtgtc
     2041 ggtcagtgtg gcctttcccg acgagcgccc atcggtcgaa ggcacagtca tcggagcgat
     2101 cccgttgaaa gatctcgctg tgatcaagat ccctcccgac gacctggatc ccgtggtcat
     2161 cggatcctct gagaacctcc ggctcggcga cgacgtcatt gcgatcggct atccgctggg
     2221 actcggcggg ccgaccgtga ctaagggaat catctccggg cgggcccggt cgatcaccac
     2281 cgatacgtcg agcggcgaag aggtcaatct cgacggattg ctccagaccg atgcttcgat
     2341 caatccgggg aactcagggg gcgccctgct cgattctgct gggcgcctga tcgggatcaa
     2401 taccgcggtg gccgggttcg cggagaacat cggcttcgct atcgcgatcg acgacgcgct
     2461 gccgacgatc gaggagatcc tgagtgagcc gccttcgcag cacgcatggc tgggagtgca
     2521 gagcgaccgc ctcgatggca ataccgccaa cgagttggga ttggatccag aagtcgaagg
     2581 agtagtcgtg gtgaacgtat tccccgagac gcccgcggaa gaagcgggca tcgaggaagg
     2641 cgatgtcatc acctcggttg gaggccagac catcacatcg cccgtcgatc tcagcgacgc
     2701 catcaccgaa cgagatcccg gcgagacggt cgagatcgaa ctggtcactc ccgacggaag
     2761 gcgtacggtc acggcgcgcc tggctcgcag gccggtgacg ctcgaaccgt aggggggcgc
     2821 gggggactgg tagcgagcgc ttgcggggga aggggccccc gcggcgtagc cgaacggacc
     2881 ccccgccagc cgggtctacg cttggccgca cctatgaagg tgaaactgag gaatcccgac
     2941 cggctcgtgg acgtgaccgg tcccaagaac gtcagggacc tccttcggga actcgacatc
     3001 gtgccggaag cagtcctggt tatccgcgac gccaccctgt tgacccgtga cgagggtctg
     3061 gccgactccg acgagctcga gatccggccc gtcctgtcgg ggggctagat cgtgaaatgc
     3121 aaggtctgcc gcgagcacgc cgtcgtggac gttcgccggc acaacgccgc gttctgcgga
     3181 gaccatttcg ttgcccacat ccactcccag gtcgagcgga cgatcaagga attcaagatg
     3241 ttcactccgg aggatcgact tctcatagcg gtctctgggg gcaaggattc cttggcggtg
     3301 tgggacgttc tcgtcgacct cggttacgac gccaccgggt tctacctgga cctcgggatc
     3361 gggggctact ccgagcgctc caaggaagct gcggtcgcgt tcgccggcgc ccgaaatcag
     3421 aagctgatca tccgctccct cgccgaagag cacggattca cggtgccgga gctgtcgcga
     3481 ttgaccggcc gggtgccgtg ctcgggttgt ggtctcaaca agcgctacga gttcaaccgg
     3541 gcggctcttg aagagggctt cgacatcctc gtcaccggtc acaacctgga cgacgaggtg
     3601 gcaacgctgt tcgggaacgt ccttcactgg aacaccgaca tgttgggacg ccaagccccc
     3661 gtgcttgaag agcgtttcct cgagggcggt ccgtcggacg ccgagcagct cggtgctcgg
     3721 cgggttctag taaggaaggt gaagccactg gtcaggatcg cggagcggga gtcggcggcg
     3781 tacgcgttga tcagagggat cgactacatc atcgaggaat gtcccatggt ggaggggaac
     3841 acccagcatc gctacaagga agcgatcacc ctgttggagg agagctcgcc cgggacgaag
     3901 caacacatgt acttcggctt cctgaagagg gccgccgacc ggttcgcgcc ggacgcggcg
     3961 gcctcggaga tcgtcgcctg cgtcgattgc ggagcgccca cgatcgtgtg ggaggaaggg
     4021 cagacgcctc ggtgctcgtt ctgtaagacg aaggcgctcg ctctgcaccg taaggagacc
     4081 ggcgaacggc ccgctccaag gcgtcggaga cggtgatgga atcggagggg gtgaaggaag
     4141 aggagcgacc gctccaagcg ggagacaggg tgttgttgat cgactcccgc gatcgcaggt
     4201 acatgatcac gctggccacc ggcaagcagt tccactctca catgggttcg atcgagcaca
     4261 acgatctgat cgggggaccg gaaggatccg tcgtcacgac cactggtggg tcgaaggtgc
     4321 ttgccttccg cccgaccctc ggggactaca ccttgaagat gaagaggggc gctcaggtcg
     4381 tctatccgaa ggacgtaggg ctgatcctcg tgtacgcaga cgtgtttccc ggagcgttcg
     4441 tcgtcgaggc cggtacggga tcgggctcgc tcacgcttgc acttgctcgg gcggtcggcg
     4501 agtcgggacg ggtgatctcg tacgagatca gggaggacca tcttgaccgc gcggtcgaga
     4561 acgtgggcgc gtggttcgaa gcagcgggag ggaaacccga
//
LOCUS       DATMED010000084         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_443644, whole genome shotgun sequence.
ACCESSION   DATMED010000084 DATMED010000000
VERSION     DATMED010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443644"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VNP73_02095"
     CDS             <1..364
                     /locus_tag="VNP73_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64739.1"
                     /translation="PEIDAVWEHLKLMNAPVLHDGEFTIDQKLIHKIAAGGFDPRSRA
                     FQTMRKVGIPGVMVTFNRMSFGVASLLGRLEATANWRAMAKELWWNEPSITPLGKKEQ
                     AWLKKAHPEFKPVLTKPR"
     gene            361..1140
                     /locus_tag="VNP73_02100"
     CDS             361..1140
                     /locus_tag="VNP73_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWL64740.1"
                     /translation="MTLFSVEWREGIAIAHLDRPPVNALDRDLLQAIGDGFADLLSED
                     AAAVVLTGEGSCFSAGADLFAVLEGSTEYIEGTVSALSAAFGSLFEFPRPVVAAVNGH
                     AIAGGAVLTCGCDYRIMAAGSGKIGISELRVGVPFPTYAIEIMRFAVGPRHIQELTYL
                     AKSYDPEVAITKGLIDEIVEPDALMERAFQVAHRLAQIPTASFEAMKKILRAPTLDRI
                     ARYGPEHDARARAGWASPEVRSSIEDFLDRTVGREKSGERG"
     gene            1150..1329
                     /locus_tag="VNP73_02105"
     CDS             1150..1329
                     /locus_tag="VNP73_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64741.1"
                     /translation="MRFRDEDRAPVIIAIGFVLGMLIFGLVLGSAIRYAGRQVGRGIC
                     RSGAGGENVVGVPCP"
     gene            complement(1388..2008)
                     /locus_tag="VNP73_02110"
     CDS             complement(1388..2008)
                     /locus_tag="VNP73_02110"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWL64742.1"
                     /translation="MDDLTVGILAFDGADELDVVGPYRTFAAVNLVREHVPSPAVRLE
                     LIAPELKQITFANGLKIEPTTDYANCPPHDLLIAPGGGSNAAEGRRKQQKNPDTLAFV
                     RERSAQARLTGSVCTGAFILAESGLLAERFVSTHWLFRTELEKLMAERGEPVRIVHER
                     VAWDEPIVTAGGVTSGIDLSLEIIEKLCGADVRKACEGELELQTPR"
     gene            2070..>2647
                     /locus_tag="VNP73_02115"
     CDS             2070..>2647
                     /locus_tag="VNP73_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64743.1"
                     /translation="MKKILVLALGVALLGALAPTGSAQDAGEGSENVKLIGSTPLPGA
                     TDMEFTKDGYAVQAVNGSGEKAGLWVVDVRNPKKPKPIGHLPCVGSGYDVGLWKNIAV
                     MSSDSASGNSSLKKDGCNVKGTDNQEGIRLVDISDRARPKEIKFVETQCGSHTNIVVP
                     RGKKAYVYVQSYPASTSGACPSAHGIISVVNIT"
BASE COUNT          487 a          840 c          850 g          470 t
ORIGIN      
        1 tcccgagatc gacgccgtgt gggagcacct gaaactgatg aacgctcccg tccttcacga
       61 cggcgagttc accatcgacc agaagctgat ccacaagatc gcggcgggcg gcttcgaccc
      121 ccgctcgcgg gccttccaaa cgatgcgcaa ggttgggatc cccggtgtga tggtcacctt
      181 caaccggatg tcgttcggag tcgcttcgtt gctcggtcga ctcgaagcga cggcgaactg
      241 gcgcgccatg gcgaaggagc tgtggtggaa cgagccttcg atcacacccc tcgggaagaa
      301 ggaacaggcg tggttgaaga aggcgcaccc ggagttcaag ccggttctca ccaaaccccg
      361 atgacgctct ttagcgtcga gtggcgcgag ggcatcgcga tcgcgcacct cgatagaccg
      421 ccggtgaatg cgctcgatcg agatctccta caagcgatcg gagacgggtt cgcagacctg
      481 ttgtcggagg atgctgccgc ggtcgtgctc accggagagg gttcttgctt ctctgccggc
      541 gcggacctgt tcgcggtcct cgagggcagc accgaataca tcgagggcac ggtgtcggct
      601 ctgtcggccg ccttcggatc gctgttcgag ttcccgcgcc ccgtcgttgc cgcggtcaac
      661 ggccacgcga tcgccggggg ggccgtgctg acgtgcggat gcgactaccg gatcatggcg
      721 gccgggtcgg gcaagatcgg catctccgag ctccgggtcg gggttccgtt cccgacctat
      781 gcgatcgaga tcatgcgctt cgccgtcgga ccgcgacaca tccaagagtt gacttacctg
      841 gcgaagtctt acgaccccga agtcgcgatc acgaagggtc tgatcgacga gatcgtcgaa
      901 cccgatgcac tgatggaacg ggcgttccaa gtggcgcatc ggctggcgca gatcccgacc
      961 gcaagcttcg aagcgatgaa gaagatcctc cgggctccca cgctcgatcg gatcgcgcgc
     1021 tatgggcccg agcacgacgc cagggcgcgc gcggggtggg catctcccga ggtgcggtcg
     1081 tccatcgagg atttcctgga caggacggtt gggcgcgaga agtcgggaga gcgtgggtag
     1141 ggtggcggca tgaggtttcg agacgaggac cgagccccgg tgatcatcgc gatcggtttc
     1201 gttctgggca tgctgatctt cggcctcgtg cttgggagtg cgatccgtta tgccggacga
     1261 caggtcggcc gcggcatctg ccgctcggga gccggaggcg agaacgtagt aggagtcccc
     1321 tgcccctaac ccccttttgt tctaactgat ggcccctata gggccctgaa ccggggtaag
     1381 aagcgactca tctcggcgtt tgaagctcca gttcaccttc gcacgctttc cgcacgtcgg
     1441 ctccacacag cttctcgatg atctcaagcg acagatcgat gccgctcgtc acacctccgg
     1501 cggtgacgat cggttcgtcc catgccacgc gctcgtgcac gatgcgaacc ggctcccccc
     1561 gctccgccat cagcttctcc agctcggttc gaaagagcca gtgcgtgcta acaaagcgct
     1621 cggccagcaa tccggattcg gccaggatga aggcgccggt acagacggaa ccggtcaatc
     1681 gcgcttgggc cgacctctcc ctaacgaacg cgagcgtgtc gggattcttc tgttgcttgc
     1741 ggcggccttc ggcggcgttg ctgccaccac cgggcgctat caataggtcg tgtgggggac
     1801 agttcgcgta gtcggtcgtc ggctcgatct tcaacccgtt ggcgaacgtg atctgcttca
     1861 gctccggggc gatcagttcg aggcgaaccg ccggcgaagg cacgtgctcg cgcaccagat
     1921 tgaccgctgc gaaggtccgg tacggcccga ccacgtcgag ctcgtcggct ccgtcgaaag
     1981 ccaggatccc cacggttaga tcgtccacag aggcgatcct gacagaccga aggcaggacc
     2041 ggcccccctg ccggtcgaac ccttagggca tgaagaagat cctcgtcctt gccctcggcg
     2101 ttgcgctgtt gggcgccctc gcaccgaccg gctcggcgca agacgcgggc gaaggctccg
     2161 agaacgtcaa gctgatcggc agcacgccgc tgccaggcgc gactgacatg gaattcacga
     2221 aagacggcta cgcggtccag gccgtcaacg gctccggcga gaaggccggc ctctgggtcg
     2281 tcgacgtcag aaaccccaag aagcccaagc cgatcggcca tcttccgtgc gtcggctccg
     2341 gctacgacgt cgggctgtgg aagaacatcg ccgtgatgtc tagcgactct gcgtcgggca
     2401 actcctccct gaagaaagac ggctgcaacg tgaaggggac cgacaatcag gaaggcatcc
     2461 ggctcgtcga catctccgac cgagcgcgac cgaaagagat caagttcgtt gagactcagt
     2521 gcggctcgca caccaacatc gtcgtgccgc ggggcaagaa ggcctacgtc tatgtacagt
     2581 cctaccccgc atcgaccagt ggtgcgtgtc cgagcgcaca cgggatcatc tcggtcgtca
     2641 acatcac
//
LOCUS       DATMED010000085        10288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4281660, whole genome shotgun sequence.
ACCESSION   DATMED010000085 DATMED010000000
VERSION     DATMED010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4281660"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VNP73_02120"
     CDS             <1..501
                     /locus_tag="VNP73_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HWL64744.1"
                     /translation="GTVDELGVNYYTTLEVEAAEGELEWPMFPGAGSFTFKLSKPTTA
                     MGWEIHPDGLFEMLVRIKREYPPISLYVTENGAAFEDTLENGDVHDSERVSYIHDHIE
                     ACRRAIAAGVELRGYFIWSLLDNFEWAEGYSKRFGIVWVDYENQKRIMKDSARWYSDF
                     ISKAGR"
     gene            complement(507..1517)
                     /locus_tag="VNP73_02125"
     CDS             complement(507..1517)
                     /locus_tag="VNP73_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWL64745.1"
                     /translation="MTDRMRLGLTLGYWGAERDDPIDLVLEAEDLGFDSVWAAEAYGS
                     DVFSPLCWIGARTSRIKLGTGIAHLFARTPASVAMTAATIDHLSHGRLILGLGVSGPQ
                     VVEGWYGQPFVKPLARTREYIEIIRKILRREGPLTYEGKHYPLPSPGGTGLGKPLKIS
                     VHPLRAELPIYLGAEGPKNVQLATEIADGWLPIFFSPYRTHVYSDSLANADDGFEIAC
                     PVTVILNDDVAEALMMVKWYLAFYIGGMGAPQQNFHLNIVSRLGFEDDAMHVQKLFME
                     GRREEAAAAVPDDLADEISLVGPVERIRERLQPWKESPVTQLLAGTRDPSALRLLADE
                     LL"
     gene            complement(1522..2994)
                     /locus_tag="VNP73_02130"
     CDS             complement(1522..2994)
                     /locus_tag="VNP73_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWL64746.1"
                     /translation="MSETLSGAEPRMLIDGKLVEAVSGKTFTNFNPATEEAIGEVSDA
                     SPDDARTAIAAARRAFDTTDWSTDHKFRRHCLEQLHGALVDAKEEFRLALIAETGSPT
                     LLTYLVQIDQPIDWFTFWPELADSYDYEGPIPDIDFMGMPHKGMIIKEPVGVVGAITP
                     FNFPLYLNLAKLGPALAAGCTIVLKPSPETPWSANLIGRLIAEQTDIPPGVVNIVTSS
                     DPAVGEVLTTDPDVDMVSFTGSTAVGKKIMAACAETVKKCHLELGGKSANILLDDADF
                     SVAAGSVPAFVCAHSGQGCALYTRLLLPRSRYDEGLELVKAGFESMTYGDPMDASNLQ
                     GPQISARQRERVLEYIETAKKEGAKVLVGGGTPDHLEKGYYVQPTILTDVDPDSTVAQ
                     EEIFGPVLVVIPFDDDDDAVQIANNSRYGLSGAVWSASEERALSVARRIRSGTIGVNG
                     GQWLHASRPFGGYRESGLGRENGVEGFEQYLETKVVALAG"
     gene            complement(3059..3946)
                     /locus_tag="VNP73_02135"
     CDS             complement(3059..3946)
                     /locus_tag="VNP73_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL64747.1"
                     /translation="MGLEGKVAIVTGAGRGLGRAEAMELASQGAKVVINDLEVGLDGG
                     DIEGTAADEVVEEIRSNGGEAVAHGGDVADFAVAEELIKFAVDQFGGLDILVNNAGIL
                     RDRMIFNMDESDWDAVIRVHLKGHFCTTRWATAYWRGKSKETGGPVYARIINTSSEAF
                     LFGSAGQPNYASAKAGIVGLTISTAQACQRYGITANAICPRALTRMTEGVGFDPEQFA
                     PENVSPLVAWLASPAAERVSGQVFVAYGSAISVVAGLSFDQRFETDGCWTVDSVQGAL
                     ESYFDKREPVSDGFAMPVQ"
     gene            complement(3950..5104)
                     /locus_tag="VNP73_02140"
     CDS             complement(3950..5104)
                     /locus_tag="VNP73_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HWL64748.1"
                     /translation="MSNSVIVEAVRTPIGKRNGALSGLHAAELLGAVQVEVVKRAGIE
                     PREVEQVVGGCVTQAGEQGLNVTRTAWLGAGLPYEVAATTVDTQCGSSQQANHMISAL
                     VGSGAIDIGIACGVEAMSRVTLGANVMNGPGSFIPPTWNLDSPDQFVGAERIAKNRGL
                     TRNDTDAFGLASQQKAARAWAEERFRREVIPIEAPETEGSSERKIVDRDGGLRETSLE
                     KLGDLKPVIEDGIHTAGTASQISDGAAAVLWMSEERARSLGLTPRARILAQAVVGSDP
                     YYLLDGPIESTRTVLKKSGIALSDIDIFEVNEAFASVVLSWASQIEPDMDKVNVNGGA
                     IALGHPVGATGARLITTALHELERSNKSLALVSMCCGGALSTASIIERIG"
     gene            complement(5101..5736)
                     /locus_tag="VNP73_02145"
     CDS             complement(5101..5736)
                     /locus_tag="VNP73_02145"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWL64749.1"
                     /translation="MKVLEKKLTPRQAQRREAVISAAMELALEGGYEAVQMREVSNRA
                     NVALGTVYRYFSSKDQLLVGVLNEWVLELQRRVETRPLQGATPAERVHDVVLRALRAL
                     DRSPSLTEALVTALSSVSAADPDAVAETQVVYENMQALVAAAMGDEGPEERDAIIRVI
                     GSVWMAALIARTRGWAGPTEMRNDLEAALHLLLPEEKNTRRAAAGNRRSKS"
     gene            5881..7038
                     /locus_tag="VNP73_02150"
     CDS             5881..7038
                     /locus_tag="VNP73_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64750.1"
                     /translation="MELELPAADDPRRLEVRAWLEEHPDPTPSDLASAGYVVPHWPQP
                     WGLDAEPLHQLIIEDELKRAGVSKPINPIGIGHCGPILVVHGTEEQKKRYIPPMLSCE
                     EIWCQLFSEPGAGSDLANTATRAERDGDEYVINGHKIWTSLAHFSKLGILLARTDPDA
                     QKHDGLSYFIIPMDVPGIEITPIIDVTGAHSFNEVFFSDVRIPADNLIGEENRGWQLA
                     KETLTNERVTLSREGVQWGWGPTARDLTDLVRDQGGLDDPILRDRLASVYIESEILRV
                     HGLRMVAAKVAGRDAPEASVRKALADPHGQRVFWLAKDLSGPAGMIQGQGPLGQSPDW
                     WDTGFLFAPALTIGGGTSEILKNVIAERILGLPRDPDPDTGKPWNETSRNR"
     gene            7035..7232
                     /locus_tag="VNP73_02155"
     CDS             7035..7232
                     /locus_tag="VNP73_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWL64751.1"
                     /translation="MRVVVDWDQCESNGKCEEVAPEVFHVAEEDDVLKVLQENPSEDL
                     RAKVEEAVKRCPKRALSVLEE"
     gene            7327..8760
                     /locus_tag="VNP73_02160"
     CDS             7327..8760
                     /locus_tag="VNP73_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL64752.1"
                     /translation="MELRRLASVVCAFALMFSAACGARLSEDQRQTGIASGGGVGAEG
                     IAEGEAGGIGSGTLGGPSSGGPGGSGGSSDIAVGGGGASGTTGGGGGAGSGVPEGGNG
                     GATDVGVTGDQITLATIADITGPQPGLFKSAHQAMQALAAFVNNEGGIYGRRLKVDAI
                     DAQTTSGGNRAAVIEACEKAFAMVGSMSAFDDGGVEEGEKCGIPDLTAIPTNPNRARA
                     SNIFSAYPNGPDHYIIGPAKYIKQKFPEAPKSAAFFWLNAPVTRSTAQLRMGVYEDLG
                     YNFKIKREVQVLEANYSPYVLEMRQEEIEYVSMVGDFQSISRFLKAMRQQNYFPTVRE
                     WDSVAYDPDFLDAAGPAGEQSKVYLNTALFEEINSNKEMQLYVGWLNRVAPGAEPDYF
                     GLYAWSAGRLFVKAATDVGPNLTRAKLMATLKGIHAWDGYGLHAPHDTGRQIPSPCYL
                     YVQVRDGRFARETPSNGWTCNQGIVRP"
     gene            8765..>10288
                     /locus_tag="VNP73_02165"
     CDS             8765..>10288
                     /locus_tag="VNP73_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL64753.1"
                     /translation="MTEFFAFSVSGIVTGAIYAVAASGLVVTYTASGIFNFAHGAIGM
                     LMAFTYWELHVQQGLPSWAALLLVVLVIAPLLGAIVERTLMRNLQGASTATSLVVTVG
                     LMVALIGAAQAIWPPGQAREMPAFGGFGGFNVGELFVTWHEAITIGVAIAVAVLLRLL
                     LFGTKLGTSMRAVVDDRSLAALTGARPGRIGMFSWSMGASLAAVAGILLAPLLQLDSL
                     ILTLLVVNAYVAAMVGRLKSLPLTFVGALILGVAQAYTVGYVQLEGALGELRSALPSL
                     LLFVVLLLLPEDKLRAARFSTLKVPSIPSFRMSLVGAAVLVGVAFYVSSLLPGSDIAR
                     VGRGLALAIVMLSLVPLTGYGGQVSLCQMTFAGIGAFAIATFGVNGSPLGLVMAGVMA
                     AGAGAIVALPALRLQGLYLALATMAFAVLMDFMFFNNPNVFGSFGGSKPVDRLALFGI
                     SFESEQSYFMLMVVMFAILGILVLALRRGTFGRRVAAMKDSPAACATLGLSLTKTKLA
                     "
BASE COUNT         1848 a         3178 c         3271 g         1991 t
ORIGIN      
        1 gggacagtgg acgagctcgg cgtcaattac tacacaacgc tcgaggtgga ggctgccgag
       61 ggtgagttgg agtggccgat gttccccgga gccggaagtt tcacgttcaa gctatcgaaa
      121 ccgaccactg cgatggggtg ggagatccat cccgacggct tgttcgagat gctggttcgg
      181 atcaaacgtg agtatccacc gatctctctt tatgtgaccg agaacggagc ggccttcgag
      241 gacacgctag agaacggtga cgtacacgac tccgagcgcg tctcttacat ccacgatcac
      301 attgaggctt gtcgccgtgc gatcgcagca ggtgtcgagc tgcgcggcta cttcatctgg
      361 tcccttctag acaacttcga gtgggccgag ggctactcga aacggttcgg catcgtatgg
      421 gtggactacg agaaccaaaa gcgcatcatg aaggacagcg cgcgctggta tagcgacttt
      481 atctcgaagg cgggtcgttg aagcggctag agaagctcgt ccgcaagcaa acgcagcgcg
      541 gatgggtcac gcgtccccgc cagcaactgt gtgaccggcg attccttcca cggctgcaaa
      601 cgttcccgta tgcgttcgac tggtccgacg agcgagatct catcggcgag atcgtcgggc
      661 acagccgccg ccgcctcttc ccgccggcct tccatgaaca gtttctgcac gtgcatggcg
      721 tcgtcctcga agcccaatcg actaacgatg tttagatgga agttctgctg gggcgctccc
      781 atgcctccga tatagaaggc cagataccac ttgaccatca tcaatgcttc ggccacgtca
      841 tcattcagga tcacggtgac cgggcacgcg atctcgaatc cgtcatcggc gttcgccagc
      901 gagtccgagt agacgtgcgt gcggtatggc gagaagaaga ttggaagcca gccgtcggcg
      961 atctcggtgg cgagttgcac gttcttcggt ccttcggctc cgagatagat cggtagttcg
     1021 gcacgcaggg gatggacgct gatcttcagg ggtttcccca gcccggtccc accgggcgac
     1081 ggcagtggat agtgctttcc ttcgtaagta agcgggcctt cgcgtctcag gatcttgcgg
     1141 atgatttcga tgtactcgcg ggtacgggca aggggtttga cgaagggttg tccgtaccaa
     1201 ccttcgacca cctgggggcc ggagacaccg agtcccagga tcaagcgccc gtgcgaaagg
     1261 tggtcgatcg tcgcggccgt catcgctacg gaggctggag tacgggcgaa cagatgggcg
     1321 atccccgtgc cgagcttgat gcgcgatgtg cgagcgccga tccaacagag gggtgagaag
     1381 acgtccgagc cgtatgcctc ggctgcccac actgaatcga agccgagatc ctcggcctcc
     1441 aggacgagat cgatcgggtc gtcgcgctcg gccccccagt acccaagggt gaggcctagt
     1501 ctcatccgat ccgtcacagt tctaaccggc gagcgctacg actttcgtct cgagatattg
     1561 ctcgaatccc tcgacgccgt tctcgcggcc gagtccgctc tcgcggtaac cgccgaacgg
     1621 acgactggcg tggagccact ggccaccgtt cacgccgatg gtgccgctcc ggatccgacg
     1681 agcaacagaa agagctcgct cttccgaggc actccacacc gcgcccgaca ggccgtaccg
     1741 ggagttgttc gcgatctgca ccgcgtcatc gtcatcgtcg aaggggatca caacgagcac
     1801 gggaccgaag atctcctcct gcgcgaccgt cgaatcggga tcgacatctg tgaggatcgt
     1861 cggctggacg tagtaaccct tctcgagatg atcgggagtt cctcctccga ccaggacctt
     1921 ggcgccctct ttctttgctg tttcgatgta ctcgagaact cgttcgcgct ggcgcgcgct
     1981 gatctgcggt ccctggaggt tggacgcgtc catcgggtcg ccgtacgtca tgctctcgaa
     2041 gcctgccttt accagctcca ggccttcgtc gtaccgagaa cgggggagca gcaaccgtgt
     2101 gtacagggca caaccctgac cagagtgcgc gcacacgaac gccggcacgg accccgcggc
     2161 gaccgagaag tctgcatcgt ccaacaggat gttcgccgac ttccccccga gttcgagatg
     2221 acacttcttc acggtctcgg cgcacgcggc catgatcttc ttgccgaccg cggtcgagcc
     2281 ggtgaagctc accatgtcta cgtcgggatc cgtggtgagt acctcgccga ccgccggatc
     2341 ggacgacgtc acgatgttca cgacgccggg cgggatgtcc gtctgttcgg cgatcagtcg
     2401 accgataaga tttgccgacc aaggcgtctc cggcgaaggc ttcagcacga tggtgcaacc
     2461 ggcggccagc gcgggaccca acttcgcgag gttcagatag agcgggaagt tgaacggagt
     2521 gatcgcgccg acgactccga ccggttcctt gatgatcatt cccttgtgag gcatccccat
     2581 gaagtcgatg tcggggatcg gtccctcgta gtcgtaggag tcggcgagct ccggccagaa
     2641 cgtgaaccaa tcgatcggct ggtcgatctg caccaagtag gtcaagagcg tcggtgagcc
     2701 tgtctcggcg atgagggcga gacggaactc ttccttggca tcgaccagcg ctccatgaag
     2761 ttgctcgagg caatggcggc ggaacttgtg gtcggtcgac cagtcggtgg tgtcgaaggc
     2821 tcgccgggcc gcggcgatcg ccgttcgagc atcgtctgga gacgcgtccg atacctcgcc
     2881 gatcgcttcc tcggttgcag gattgaagtt ggtgaaggtt ttgcccgaaa ccgcctccac
     2941 gagcttgccg tcgatcaaca tgcggggctc ggccccactc agtgtttcgc tcatcgcagc
     3001 ctcccgttct ctctcggggt ccgcgaccgc aagcggtcgc tacgagcccc cgaacccctt
     3061 actgaaccgg catagcgaaa ccgtcgctga ccggctccct cttgtcgaaa tacgactcca
     3121 gtgccccttg taccgaatcg accgtccagc agccgtcggt ctcgaaacgt tgatcgaatg
     3181 acagacccgc gaccacagag atggcactcc cgtaagcaac gaagacctgt ccagagactc
     3241 gctcggccgc gggtgaggcg agccatgcca cgagcggtga aacgttctcc ggcgcgaatt
     3301 gttcgggatc gaacccgacc ccttctgtca tgcgggttag tgcgcgcggg cagatcgcgt
     3361 tcgcggtgat cccatagcgc tggcacgcct gcgcggtgga gatcgtcaga ccgacgatcc
     3421 cggccttagc cgacgcgtaa ttcggctggc ccgcagatcc gaacaagaat gcttccgagg
     3481 acgtattgat gatgcgtgca tagacgggac cgcccgtttc ctttgacttc cccctccaat
     3541 atgcggtcgc ccatcgggtg gtgcagaagt gtcccttgag gtgcaccctg atcacggcat
     3601 cccagtccga ctcgtccatg ttgaagatca tgcggtcgcg gaggatcccc gcgttgttga
     3661 ccaggatgtc gaggcccccg aactggtcga cggcaaactt gatcagctct tcggctaccg
     3721 cgaaatcggc aacatccccg ccgtgtgcga ccgcttcgcc accattcgaa cggatctcct
     3781 ctacgacttc atctgccgca gtcccctcga tatctccgcc gtctaggccg acctcgaggt
     3841 cgttgatcac gaccttggcc ccctgagacg ccagctccat cgcttccgcg cgcccaaggc
     3901 cgcgtcccgc gccggtaacg atcgctacct tgccctcgag tcccatcgct tatccgatcc
     3961 tttcgatgat gctcgcggtc gacagtgctc caccgcaaca catcgatacg agtgcgaggg
     4021 acttgttcga gcgctccagc tcgtggagtg ccgtggtgat caaccgagct ccggtagcac
     4081 cgacggggtg gccgagtgcg atcgcaccgc cgttcacgtt caccttgtcc atgtctggct
     4141 cgatctggga cgcccacgag aggacgaccg aggcgaacgc ttcgttcacc tcgaagatgt
     4201 cgatgtcgct taaggcgatc ccagacttct tcaggaccgt ccgggtcgat tcgatcggac
     4261 cgtcgagtag gtagtaggga tcggagccga cgaccgcttg cgccaagatc cgagcgcgag
     4321 gggttagacc gagagaccgg gcgcgttcct ccgacatcca caacacggca gccgcgccgt
     4381 ccgagatctg tgatgcggtc ccggcagtgt ggatgccatc ctcgatgacc ggcttcaggt
     4441 cgcccaactt ctccaggctg gtctcgcgca gtcccccatc gcggtcgacg atcttgcgtt
     4501 ccgaacttcc ttcggtctcc ggagcttcga tcgggatcac ttcccggcgg aacctctctt
     4561 cggcccacgc ccgtgcagcc ttctgttgcg aagcgagacc gaaggcatcc gtgtcgtttc
     4621 gagtgaggcc gcggttcttg gcgatgcgct cggcccccac gaactgatcg ggggagtcga
     4681 ggttccaagt gggcgggatg aacgaaccgg ggccgttcat cacgttcgct cccagggtga
     4741 cgcggctcat cgcctcgacg ccgcaggcga tcccgatgtc gatcgcgccc gatccgacga
     4801 gcgccgagat catgtggttc gcctgttgcg acgatccaca ctgggtgtcg acggtggtag
     4861 ccgcgacctc gtatggcaga cctgcgccca accaggcggt ccgcgtcaca ttgagaccct
     4921 gctcgccggc ctgcgtaacg catccaccca cgacttgctc gacctcacgc ggctcgatcc
     4981 cggcgcgctt aaccacctcg acctggacgg cgcccagcag ttcggccgca tggagaccgg
     5041 acagggcccc gttccgcttc ccgatcgggg ttcgaacggc ttcgacgatg acgctgttgc
     5101 tcatgacttc gacctccggt tgcccgctgc tgctcgacgc gtgttctttt cttcggggag
     5161 taatagatgg agggcagctt ccaggtcgtt gcgcatctcg gtcggaccgg cccaccctcg
     5221 cgtgcgggcg atcaacgccg ccatccacac cgaaccgatc accctgatga tcgcgtcccg
     5281 ctcctcgggc ccctcgtcgc ccatcgcggc ggccacgagg gcctgcatgt tctcgtacac
     5341 gacctgcgtt tcggcaaccg catcgggatc ggcggccgaa accgacgaca gggctgtgac
     5401 cagggcttcg gtcagagagg gggagcgatc gagcgcccgc agcgcccgca atacaacgtc
     5461 gtggacccgc tcggccggtg tcgctccctg cagcggacgt gtctctaccc gacgctgtag
     5521 ctccaggacc cactcgttga ggacgccgac gaggagctgg tccttcgagg agaagtagcg
     5581 gtaaacggtt ccgagcgcga cgttcgcacg gttggacacc tcgcgcatct ggacggcctc
     5641 gtagcctcct tcgagcgcga gctccatggc agcactgatg acggcctcgc gccgctgtgc
     5701 ttgtcgggga gtgagcttct tttcgaggac cttcacggag aggtcagcgt agcggaaact
     5761 agaacgtgtt gcaatttttc aggcgcactc ctatagtgcg gcgatggagc gatgggctcg
     5821 gccatcgcgg tccgtgcatc gaggccgcgg cgggaaagtc ggcaccgtag gagtcaactg
     5881 atggagttgg agctcccggc ggcagacgat cctcgccggc tcgaagtgcg tgcgtggctc
     5941 gaagagcacc ccgatccgac tccgtccgat ctagcttccg ccggatacgt cgttccgcac
     6001 tggccccaac cgtggggact cgacgcggag ccactccacc aactcatcat cgaagacgag
     6061 ttgaagcgcg ccggtgtgtc gaagcccatc aaccccatcg gcatcggcca ctgcggcccg
     6121 atcctcgtcg tgcacggcac cgaggagcag aagaagaggt acatcccgcc gatgctgtcg
     6181 tgcgaggaga tctggtgcca gctgttcagc gaaccggggg ccggctccga cctcgcgaac
     6241 actgccacga gggccgaacg tgacggtgac gagtacgtca tcaacgggca caagatctgg
     6301 acgtcgctgg ctcatttctc caagctcggg atcctgctcg cccgcaccga ccccgatgcg
     6361 cagaaacacg atgggctctc ctacttcatc atcccgatgg atgtcccggg gatcgagatc
     6421 actccgatca tcgatgtgac cggcgcgcac agcttcaacg aagtcttctt ctccgatgta
     6481 aggatcccgg ccgataatct gatcggtgaa gagaaccgcg gctggcagct cgccaaagag
     6541 accctgacga acgagcgcgt gaccctgtcg cgcgaggggg tgcagtgggg ttggggtccg
     6601 actgctagag acttgaccga cctcgtgcgc gaccaaggtg gtctcgacga tccgatcctg
     6661 cgcgaccggc tcgcctcggt ctatatcgag agtgagatcc tgcgcgtcca cggtctacgg
     6721 atggtggcgg ccaaggttgc aggcagggac gcgccggaag cctcggtgcg caaggccctc
     6781 gccgatccgc acggtcaacg cgtgttctgg ttggcgaagg acctctcggg tccggcgggg
     6841 atgatccagg gtcaaggacc gctgggtcag agccccgatt ggtgggacac tggtttctta
     6901 ttcgctcccg cactgacgat cggaggcggt acttccgaga tcctcaaaaa cgtcatcgcc
     6961 gaacgaatcc tcgggctgcc gcgcgacccc gatccggata ccggtaagcc gtggaacgaa
     7021 acgagccgta atagatgaga gtcgttgtcg attgggacca gtgtgagagc aacgggaaat
     7081 gcgaggaggt cgcgcccgag gtgttccacg tggcggagga ggacgacgtc ctcaaagtgc
     7141 tgcaagagaa cccatccgaa gacctaaggg cgaaggtcga ggaggcggtg aagcggtgcc
     7201 ctaaacgggc tttgtccgta ctagaggagt agaggtgaaa gtaggaggga gggggaacca
     7261 gtgaggccgc ttgcggccgg ggttcccaga gaaggggtgg cgcgctagca ggggggagtt
     7321 gttctgatgg aactgcgacg gctggcatcg gtggtgtgtg cgttcgcgct catgttctct
     7381 gccgcgtgcg gagcccgtct ctcagaggac caacgccaga cgggcatcgc gtccggcggc
     7441 ggcgtcggtg cagaagggat cgccgaaggt gaggctgggg ggatcggttc cgggaccctc
     7501 gggggcccca gtagtggcgg ccccggggga tcgggcggtt cttccgatat cgctgttgga
     7561 ggtggcgggg catccggcac caccggtggc ggaggcggcg cggggtccgg tgttcctgaa
     7621 ggcggcaacg gtggtgcgac agatgtcggg gtgaccggcg accagatcac gctcgcgacc
     7681 atcgcagaca tcaccggccc gcaaccgggt ctcttcaagt ccgcgcatca ggcgatgcaa
     7741 gcccttgccg cgttcgtcaa caatgagggc gggatctacg ggcgcaggct caaggtcgac
     7801 gcgatcgacg cccagaccac ctcgggcggc aatcgtgccg cagtgatcga ggcgtgtgag
     7861 aaggcattcg ccatggtcgg gtcgatgtcg gctttcgacg atggcggtgt cgaggagggg
     7921 gagaagtgtg ggatcccgga cctcaccgcg atcccgacga accccaaccg ggcacgggct
     7981 agcaacatct tttccgccta cccgaacggt cccgaccact acatcatcgg gccggcgaaa
     8041 tacatcaagc agaagttccc ggaggcaccg aagagcgcag cgttcttctg gctcaacgct
     8101 ccagtaacca gatccacggc ccagctccgc atgggggtgt acgaagacct cggctacaac
     8161 ttcaagatca aacgtgaggt ccaggtcctc gaggccaatt acagccccta cgtactcgag
     8221 atgcgtcagg aagagatcga gtacgtgagc atggtcggcg acttccagag catctcgcgg
     8281 ttcctcaagg cgatgcgaca gcagaactac ttccccacgg tgcgcgagtg ggattcggtt
     8341 gcctacgacc cggacttcct cgacgcggca ggcccggccg gcgaacaatc aaaggtctat
     8401 ctcaacaccg ctttgttcga ggagatcaac tccaacaaag agatgcagct gtacgtgggc
     8461 tggctgaatc gtgttgctcc gggagccgag cccgattact tcggtctcta tgcatggtcg
     8521 gccggtcgac tgttcgttaa ggctgcgacc gacgtcggac cgaacctgac tcgcgcgaag
     8581 ctgatggcta cattgaaagg gatccatgcg tgggacggat atggactcca cgctccacac
     8641 gacaccggtc ggcagatacc gtcgccgtgt tacctctacg tccaggttcg cgacggcaga
     8701 ttcgcacgag aaacaccttc gaacgggtgg acgtgtaacc agggcatcgt aagaccgtga
     8761 tctcgtgacg gagttcttcg cattctccgt ttccggcatc gtcacgggtg ccatctatgc
     8821 ggtggcggcg agcgggctgg tcgttaccta cacggcttcc gggatcttca acttcgcgca
     8881 cggcgcgatc gggatgctga tggcattcac ttattgggag cttcacgttc agcaaggctt
     8941 gccgtcgtgg gcggcgcttc tgctcgtggt gttagttatc gctcctttgc tgggagcgat
     9001 cgtcgagcga acgctgatga ggaaccttca aggggcttcg acggccactt ctctggtcgt
     9061 caccgtcggc ttgatggtgg ctttgatcgg cgctgcccaa gcgatctggc ctccgggcca
     9121 agcgcgcgag atgccggcct tcggcggatt cggtggcttc aacgtcggcg agctattcgt
     9181 cacctggcac gaagcgatca cgatcggcgt tgcgatcgcg gtcgcggtcc tattacggct
     9241 gcttctattc ggcaccaagc tcggcacgag catgagagcg gtcgtcgacg atcgatcgct
     9301 cgctgcatta acgggggcac gccccggacg gatcgggatg ttcagctggt cgatgggagc
     9361 gtcgctcgct gccgtcgcgg ggatcctgct ggctccgctg ctccagctcg actcactgat
     9421 cctcaccttg ttggtggtga acgcctatgt ggcggccatg gtgggtcggc tgaaaagcct
     9481 tccgctcaca ttcgtcggtg cattgatcct cggtgtggcg caggcctaca cggtcgggta
     9541 cgtacagctc gaaggcgcgt tgggcgagct gcgttctgca cttccgtcgc tgctgctgtt
     9601 cgtcgttctg ctcttgctcc ccgaggacaa gttgcgggcg gcacgcttca gcacgctgaa
     9661 ggtcccctcg atcccatcgt tccggatgtc cctcgtaggg gcggcggtgt tggtgggcgt
     9721 tgccttctac gtttcgagcc ttctgcccgg atccgatatc gcacgcgtcg gacgcggcct
     9781 cgccctggcg atcgtgatgc tttcgttggt tccgctgacg ggttacgggg gccaggtctc
     9841 cttatgtcag atgacgttcg ccgggatcgg cgcgttcgcg atagcgacct tcggcgtgaa
     9901 tgggtcgccg ctcggtctcg tcatggccgg agtcatggca gctggcgccg gagcgatcgt
     9961 ggccctgccg gcactcaggc tgcagggtct ctatctggcg ctggcgacga tggcgttcgc
    10021 cgtcctgatg gacttcatgt tcttcaacaa cccgaatgtg ttcggctcat ttggtggtag
    10081 caagcccgtc gacagactgg ctctgttcgg catctctttc gagagcgagc agtcctactt
    10141 catgctgatg gtcgtcatgt tcgcgatcct tgggatcctg gtgctcgcgc tgagacgtgg
    10201 gacgttcggt cggcgggtcg cagcgatgaa ggacagcccg gccgcatgcg cgacgttggg
    10261 gctgagtctt acgaagacga aactcgct
//
LOCUS       DATMED010000086         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2754155, whole genome shotgun sequence.
ACCESSION   DATMED010000086 DATMED010000000
VERSION     DATMED010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2754155"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VNP73_02170"
     CDS             complement(<1..1081)
                     /locus_tag="VNP73_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWL64754.1"
                     /translation="MAQPRQADPPDPLGARRELETGAGPVAIYSLATLEDSGAGAKLA
                     LIPNTVKVLLENLLRTAGTPHASEDIVEWLAGWGQKPLEDREFPFAPARVILQDFTGV
                     PAVVDLAAMRAALDRAGGDPAKVDPLVPVDLVVDHSVQVDAFASSGAFRFNVDREYER
                     NGERYSLLKWAQQAFAGFRVVPPGMGIVHQVNLEHLGTVVRLQEEQSGRAAIPDTLVG
                     TDSHTPMINGLGVLGWGVGGIEAEAALLGQPLPMVTPKVVGFRFSSELQIGVTATDLV
                     LTVTEMLRKHGVVGKFVEFYGEGLTGLTVADRATLSNMSPEFGATASLFPVDDQTLDY
                     MRLTGRKGDQIDLVERYAKEQGMFRT"
     gene            1172..2122
                     /locus_tag="VNP73_02175"
     CDS             1172..2122
                     /locus_tag="VNP73_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL64755.1"
                     /translation="MMVSIDVRRTAAVLSASLLIVLALPVGATAATARCRGMKATVVG
                     TAGDDQLGGTDGNDVIVGKGGADEISASGGNDVICGGGGPDAIIGGSGRDKISGGAGG
                     DVLVPGSDDDSVAGGKGFDYIDYIDADSGVTIDMSARRTTGSGVDRFNGIEGAVGSGQ
                     NDTLVGSDAVDDLIGGSGNDALFGKGENDLLHGGFGDDSLDGGDGAQDVASFAGAASA
                     VTADLADGTASGEGADTLSNVEGLAGSDNGDTFRGNDGSNGLFGGLGGDTLDGRGGND
                     VVRGEAGDDNLDGGAGTDSLDGGDGTDACANGEQANACES"
     gene            complement(2142..>2321)
                     /locus_tag="VNP73_02180"
     CDS             complement(2142..>2321)
                     /locus_tag="VNP73_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HWL64756.1"
                     /translation="AFGLGDFEWILAFEADGLERIVDLIRRLRSTEARRYTKVEIPFV
                     TGIRKSLEETIQDLT"
BASE COUNT          419 a          708 c          785 g          409 t
ORIGIN      
        1 tcgttctgaa catgccctgc tctttcgcgt agcgctcgac cagatcgatc tgatcgccct
       61 tccgtccggt cagccgcatg tagtcgagtg tttgatcgtc gaccgggaac agggacgcgg
      121 ttgctccgaa ctccggcgac atgttcgaaa gcgtggctcg atccgcaacc gtcagacccg
      181 tcaggccctc gccgtagaac tcgacgaatt tgcctacgac cccatgcttg cgcagcatct
      241 cggtgaccgt aagaacgagg tcggtggcgg tcactccgat ctggagctca cttgagaacc
      301 ggaaaccaac gaccttcggc gtcaccatcg ggagtggctg gcccagcagt gcagcctcgg
      361 cctcgatccc gccgaccccc cagccgagca cacccagccc gttgatcatc ggtgtgtgcg
      421 agtcggtgcc gacgagcgtg tcggggatgg ccgctcgtcc actctgctct tcctggagcc
      481 ggacgaccgt gccgagatgc tcgaggttca cctgatggac gatgcccatc cccggcggaa
      541 cgacccggaa ccctgcgaaa gcctgctgcg cccacttcag aagcgaatag cgctcgccgt
      601 ttcgctcgta ctccctgtcg acgttgaaac ggaatgctcc cgaactggcg aacgcatcga
      661 cctgcacgga gtggtccaca acgagatcca cgggcacgag cgggtctacc ttggccgggt
      721 cgccgccggc gcgatcgagc gcagctcgca tagcggcaag gtcgacgacg gccgggaccc
      781 ccgtgaagtc ctggaggatc actcgagccg gagcgaatgg gaactcgcgg tcctctagag
      841 gcttctgccc ccatcctgcg agccactcga cgatgtcctc agaggcgtga ggggtgccgg
      901 cggtgcgcaa caggttctcg aggaggactt tgaccgtgtt cggtatcagc gcaagcttcg
      961 cacccgctcc ggagtcctcc agcgttgcga gcgaatagat agcgaccggg ccggcgcccg
     1021 tttcgagctc gcgcctggcc cccagtgggt cgggtgggtc cgcctgccta ggttgcgcca
     1081 tcgcccccat cataggcagt gcgacggagt cggcaggagc gaaggtcgct tagccttgcg
     1141 atgattctgg cggatcaaca ggaggttcaa catgatggtg tccatagatg tgcgccggac
     1201 ggctgccgtt ctcagcgcat ccttattgat cgttcttgcc ttaccggtgg gagcgacggc
     1261 tgcgaccgcg agatgccggg gaatgaaagc caccgtcgtc ggcaccgccg gcgacgacca
     1321 gctgggcggc acggacggca acgatgtgat cgtggggaag ggtggtgcag acgagatctc
     1381 tgcttccggt ggcaacgacg tcatatgcgg cgggggaggc cccgacgcga tcatcggggg
     1441 cagcgggcgc gacaagatct cgggtggcgc cggtggtgac gtgctcgttc ccggttcgga
     1501 cgacgactcc gtggccggcg gaaagggctt cgactacatc gactacatcg atgcggattc
     1561 tggagtgacg atcgatatgt ccgctcgccg gaccacaggc tccggggtcg atcggttcaa
     1621 cggcatcgag ggcgcagtcg ggtcgggcca aaacgacacg ctggttggct cggatgccgt
     1681 ggacgacctc atcggcggaa gcggaaacga cgctctcttc ggtaaaggtg agaacgatct
     1741 cctgcacggt gggtttggag atgactccct ggacggcggg gacggagccc aggacgttgc
     1801 ttcgttcgcg ggggcggcaa gtgcagttac cgccgatctc gccgacggca ccgcttcggg
     1861 ggaaggcgcc gacactttgt cgaacgtcga gggtctcgcg ggctcggaca atggagacac
     1921 gttcagaggc aacgacggct ccaacggttt gttcggcgga cttggcggtg acacgctcga
     1981 cggtcgcgga ggcaacgacg tcgtccgggg cgaggccggc gacgacaatc ttgacggcgg
     2041 agcgggaacc gattcgctgg atggtgggga tggaaccgat gcgtgcgcca acggcgaaca
     2101 agcgaacgcc tgcgagagct aacgcgactt agctcccgaa gctaggtgag gtcctggatc
     2161 gtttcttcca ggctcttgcg gatcccggtc acgaagggga tctccacttt ggtgtaacgg
     2221 cgcgcctcgg ttgagcggag cctgcgtatc agatcgacga tccgttcgag accgtcggct
     2281 tcgaacgcga ggatccattc gaagtcaccg aggccgaagg c
//
LOCUS       DATMED010000087         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_75399, whole genome shotgun sequence.
ACCESSION   DATMED010000087 DATMED010000000
VERSION     DATMED010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75399"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VNP73_02185"
     CDS             <1..530
                     /locus_tag="VNP73_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64757.1"
                     /translation="PAVCSAFSPGATGSGKPTVVVTDTATEQQPVIQKVNLAESISDL
                     DVIPTGLLPPPTDAFNIQVDSANPSAGLHVLFEFPTRRDYDLELLWPDDSYAARSHDF
                     NLVYSPAGIHENGGHAGVATDASEHIVGVATPDCGGYTVETVNWLGEGGEMEVKVWLG
                     TADNVPQAPGAEPHA"
     gene            636..1157
                     /locus_tag="VNP73_02190"
     CDS             636..1157
                     /locus_tag="VNP73_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL64758.1"
                     /translation="MAEIVVREATDADIEPARSALRSAYAEYEKSFPPENWAPYLTDI
                     LDLEGRASESELLVAESDGSVVGCVSYFPPGSKASYPSDSFSERWPDNWGAFRLLAVD
                     PSARGHGVGRRLTDECIERARGKGAPFLGLHTTAPMAIARSMYERMGFERVVQYDFRP
                     GPTVLVEAYRLLL"
     gene            complement(1162..>2220)
                     /locus_tag="VNP73_02195"
     CDS             complement(1162..>2220)
                     /locus_tag="VNP73_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWL64759.1"
                     /translation="RILLVDDEATVLRNLERLLRLHGYDCKSARSAAEARATLDIDDG
                     YSLILTDVNMPGESGIELLEQVAKAHPDIATVMVTGIDDRNLAERALAMGAYGYVIKP
                     FEPNEILIGISNALRRRTLELENRKHRTQLEQMVDERTTDLWDAIRQLETAGKELRLS
                     REETIQRLAVAAEFRDDETGKHIERMSRYCALLARKIGGDYEGCELIRVATVMHDVGK
                     IGIPDSILLKPGPLSSAEFEVMKTHAEIGKMILEGSEAELLQMATAIAATHHEWWDGS
                     GYPSGLNAEEIPLEGRIAAIGDVFDALTSNRVYRKAFTLGEAVEIMKKERGTHFEPFL
                     LDLFFDSIQELVDIKAAH"
BASE COUNT          388 a          733 c          656 g          443 t
ORIGIN      
        1 cgcccgccgt atgttccgcg ttctctcccg gtgcgacagg ctccggaaag ccgaccgtcg
       61 tcgtgaccga caccgcgacc gagcagcagc ccgtcatcca gaaggtgaac ctggccgagt
      121 cgatcagcga cctcgacgtc atccccacgg gactgctccc gccccctacc gatgcattca
      181 acatccaagt ggactctgcc aatccctcgg ccggccttca cgttctgttc gagttcccga
      241 cgcggcgtga ctacgacctc gagctgttgt ggcccgatga ttcgtacgcc gcccgctcgc
      301 acgacttcaa cttggtgtac agccccgcgg gcatccatga gaacggtggc cacgccggag
      361 tcgctaccga tgcttccgag cacatcgtcg gcgttgcgac ccccgattgc ggtgggtaca
      421 cggtcgagac cgtcaactgg ctgggtgaag gtggcgagat ggaggtcaag gtatggctcg
      481 gcactgcgga caacgtgccg caagcccccg gagcagagcc ccacgcctaa ccgttctttc
      541 tgcacatctg gcccccgttc tgaggggcca acctagaaaa gaaactcggc ggcccccttc
      601 ggggggccgc ttctttttat gcaacgattc gcgccatggc ggagatagtg gtcagagaag
      661 caaccgacgc ggacatcgag ccggcccgca gcgctctgcg atcggcctat gccgagtacg
      721 agaagtcttt cccacccgag aattgggctc catacctcac cgacatcctc gacctcgaag
      781 ggcgcgcgag cgagtccgaa cttctagtgg cggaatcgga cggcagcgtg gtgggttgcg
      841 tcagctattt ccctcccgga tcgaaggcct cctacccatc ggattcgttc tccgagcggt
      901 ggcccgataa ctggggagcg ttccggctgc tcgccgtaga tccctccgca cgcggtcacg
      961 gtgtcggacg ccgattgacc gacgaatgca tcgaacgcgc ccgcgggaaa ggagcgccgt
     1021 tcctcggcct ccacacgacc gcaccgatgg cgatcgcgcg atccatgtac gaacggatgg
     1081 gcttcgaacg agtggtccaa tacgacttcc gcccgggccc gacagtcctg gtggaggcct
     1141 atcggctact cctgtgaaaa gttagtgcgc ggccttgatg tcgactagtt cttgtatgga
     1201 gtcgaagaag aggtcgagca ggaacggctc gaagtgcgtg ccccgctcct ttttcatgat
     1261 ctctacggcc tcacccaggg tgaacgcctt ccgatagacg cggttcgagg tcaacgcgtc
     1321 gaagacgtct ccgatggccg cgatcctccc ctccagtggg atctcctctg cgttcaatcc
     1381 actcggatag ccgcttccgt cccaccattc gtggtgcgtg gcggcgatcg cagtggccat
     1441 ctgaaggagc tcggcctcgg atccctcgag gatcatcttc ccgatctcgg catgggtctt
     1501 catcacttcg aactccgcgc tcgaaagggg gccgggtttg agcaagatgc tgtcggggat
     1561 cccgatcttc ccaacatcgt gcatcacggt tgcgactctg atcagctcgc aaccctcgta
     1621 gtcaccgccg atctttcgag ccagtagcgc gcagtagcgg ctcatcctct cgatgtgctt
     1681 tccggtctcg tcgtcacgaa actccgccgc taccgccagt cgttggatcg tttcctcgcg
     1741 tgaaaggcga agttccttgc cggccgtctc gagctgccgg atcgcatccc acaggtcggt
     1801 ggttcgctcg tccaccatct gctcgagctg ggtccggtgc ttcctgttct ctagctcgag
     1861 ggtccggcgc cggagcgcgt tcgagatccc gatcaagatc tcgtttggct cgaagggctt
     1921 gatcacatac ccatacgccc ccatcgcgag cgcccgttcg gcaaggttgc ggtcgtcgat
     1981 cccggtcacc atcacggtcg cgatatccgg atgagcttta gcgacctgct ccagcaattc
     2041 gatgcccgac tcccccggca tgttcacgtc cgtgaggatg agggagtacc catcgtcgat
     2101 gtccagagtg gcccgagctt cggccgccga gcgagcgctc ttgcagtcgt aaccgtgcag
     2161 ccgaaggagc cgttcgaggt tcctgaggac ggtggcctca tcatcgacga gcagtatgcg
//
LOCUS       DATMED010000088         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2377613, whole genome shotgun sequence.
ACCESSION   DATMED010000088 DATMED010000000
VERSION     DATMED010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377613"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VNP73_02200"
     CDS             complement(<1..661)
                     /locus_tag="VNP73_02200"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HWL64760.1"
                     /translation="MAKKKRKKSRPEGVDPNQKRRERLEARRLAKNEALRAQHRARTR
                     ERIVRWIVIAGIAAFAVWFFFVRGQAPDAIAGHRIEHASTSGAGDHVETVVTYDTNPP
                     VSGPHSNRAAPCGIFASDIPDENEVHALEHGAVGILYKPDIEVEEIERIETIVRSYDS
                     HVLSAPDSDMTRNVVVTAWGHLMRLDSVDEPAIREFIDYFRQGGDAPEAFQECPNTSN
                     ES"
     gene            692..1042
                     /locus_tag="VNP73_02205"
     CDS             692..1042
                     /locus_tag="VNP73_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64761.1"
                     /translation="MDEEMWKILLIAALALNAAVGFGYRVYRLSKGGPIADVWGQAIL
                     GALLAAIAVGIGVGAVWLRWVAFGYSLLFGLLAMPIWVLGVLLPMRPRAIDYAFTCVY
                     WLILVAIGIAAVAM"
     gene            complement(1113..1595)
                     /locus_tag="VNP73_02210"
     CDS             complement(1113..1595)
                     /locus_tag="VNP73_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWL64762.1"
                     /translation="MDYSKALGDRLRNIRVQKGMSLQDVQQASKGKWKAAVVGAYERG
                     DRNVTVARLSELADFYGVPVSEILPEDGGTMAPSAAEARRRVVLDLEGLKKVPEHDRD
                     PLSRFTHAIQIQRGDYNGRVLTIREDDLMALALLYQTTADELSRRLQEWGLIATNGSH
                     "
     gene            1774..2622
                     /locus_tag="VNP73_02215"
     CDS             1774..2622
                     /locus_tag="VNP73_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWL64763.1"
                     /translation="MRALWKGAITFGLISIPVRLYSAVSEKGLKFHLLHDEDGGRIKY
                     QRTCSKCGKEVTWDDIVKGYEYSKDHYVQFTDEELQAVELDTVRAIDVVSFVPLVDID
                     PIYFNKTYYVAPEPSGLKAYRLLQEALEAEQQVGIAKVALRDNKEHLATVRLMDQNGR
                     PVFVLETMHWPDEIREPEFEELDKNVQVRDAEVKMARQLVQQLADDFKPEDFQDEYRQ
                     ALEKLVEKKVEGEEITVAATPEEEPTKVVDLMEALKASVEEAKKKRAPAAAKKKTTTR
                     RKASAS"
     gene            2640..3017
                     /locus_tag="VNP73_02220"
     CDS             2640..3017
                     /locus_tag="VNP73_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWL64764.1"
                     /translation="METVSIKIPASPAYIQVVRLVAAGLASRLKFTIDEIEDLKIAVD
                     ELSAYVTGAQGRDGTLEISFAVSEDRIEISGVGRLAPGQKVRTDLTEFSQMILATVAD
                     EASLQQPDGTPAFTLVKTRKSNG"
BASE COUNT          601 a          937 c          934 g          593 t
ORIGIN      
        1 acgattcgtt cgaggtgttc gggcactcct ggaacgcctc cggcgcgtcg cctccctgac
       61 ggaagtaatc gatgaactca cgtatcgcgg gctcgtcgac ggagtcgagc ctcatcagat
      121 gtccccacgc ggtgacgacg acgttcctgg tcatgtcgga atccggtgcg gagagaacgt
      181 gagagtcgta gctccgaacg atcgtctcga tgcgctcgat ctcttcgacc tcgatgtccg
      241 gcttgtagag gatcccgacg gcgccgtgct cgagcgcgtg cacctcgttc tcatcgggga
      301 tatcgctggc gaagatccca caaggtgcgg cgcggttcga gtgcggtccg gacacggggg
      361 ggttcgtgtc gtaagtgacg accgtttcga cgtgatcacc tgcacccgac gtactagcgt
      421 gctcgatcct gtgtcccgcg atcgcgtcgg gtgcttgtcc gcgcacgaag aagaaccaca
      481 ctgcgaacgc tgcgatcccg gcgatgacga tccaccgaac gatccgctct cgagttcgcg
      541 cgcggtgctg tgctcgcagg gcttcgttct ttgcgagacg tctagcctcg aggcgttctc
      601 tccttttctg gttcggatcg acgccctcgg gtctggactt cttgcgcttc ttcttcgcca
      661 cgagggcgga ggctatcttg aacagccgcc tatggacgaa gagatgtgga agatcctcct
      721 tatcgcagca ctcgccctca acgctgccgt gggcttcggc taccgcgttt acaggctgag
      781 caaggggggt ccgatcgcgg acgtgtgggg tcaggcgatc ctgggagccc tgctggcggc
      841 catcgccgtt gggatcggag tgggggccgt ctggctgcgc tgggttgcgt tcggctactc
      901 gctgctcttc gggctgctcg ccatgccgat ttgggtcctc ggagtgctcc tcccgatgag
      961 accacgcgcc atcgactacg cgttcacgtg tgtgtattgg ctgatactgg tggcgatcgg
     1021 gatcgcggcc gtggctatgt aacccgaagc gcggcggcct cggccgtggc tatgtaaccc
     1081 gaagcgcggc ggcctcggcc gtcgcgcaag acttagtggc taccgttggt cgcgatcaat
     1141 ccccactcct gtagccgtcg cgacagctcg tccgccgtcg tctggtacag aagcgcgagc
     1201 gccatcagat cgtcttcccg gatcgtgaga acccgaccgt tgtagtcccc tcgctgtatc
     1261 tggatcgcgt gagtgaaacg agaaagcgga tcccgatcgt gctcgggaac cttcttgaga
     1321 ccttcgagat cgagcacaac cctccggcgc gcttccgcgg ccgagggagc catggtccct
     1381 ccgtcctcgg gaaggatctc ggagacggga accccgtaga aatcggcgag ctctgacagt
     1441 cgggcgacgg tcacattgcg atcgccccgc tcgtaggcgc cgaccacggc cgccttccac
     1501 ttgcctttcg acgcttgctg cacgtcctgg agcgacattc ccttctgaac cctgatgttt
     1561 ctcaggcggt cgcccaatgc cttggaatag tccacagcaa aaatctcctt ggtctgtttt
     1621 cccggcaggt ccgccccggc cggcttgatt tgtataaccc ctatcttcga cgcggagagt
     1681 gaagtacctc tccattttag taactttccg cattcgtcgc ccgtgaaacc aaggggaaag
     1741 agattgcctt acctcccccc tagaatgaca gccatgaggg cgctttggaa aggtgcgatc
     1801 acattcgggc tgatcagcat ccccgtgcgc ctgtactcgg cggtctctga aaaaggcctg
     1861 aaattccacc ttctccacga tgaggacggg ggccggatca aataccagag gacctgctcg
     1921 aagtgcggca aggaggtcac ctgggacgac atcgtcaagg gctacgagta ttcgaaggac
     1981 cactacgtgc agttcacgga cgaggagctt caggccgtgg agctggatac cgtccgagcc
     2041 atcgatgtgg tgtctttcgt gccactcgta gacatcgacc ccatctactt caacaagacc
     2101 tattacgtcg cccccgagcc atccggtctc aaggcctacc ggttgcttca agaggcactc
     2161 gaggcggaac aacaggttgg tatcgccaag gttgcactca gggacaacaa ggaacacctt
     2221 gcaaccgtgc gtctcatgga ccagaacggt cggcccgttt tcgtgctgga gacgatgcat
     2281 tggccggacg agatccgtga gcctgagttc gaagagctgg acaagaacgt tcaggtacga
     2341 gacgcagagg taaagatggc gcgacagctc gtccaacaac tcgccgatga cttcaagccg
     2401 gaggacttcc aggacgaata ccgccaagcg ttggagaagc tcgtcgagaa gaaggtggaa
     2461 ggcgaggaga tcacggtcgc cgcgacgccc gaggaagagc ccactaaggt cgtcgacctg
     2521 atggaggcac tgaaagcctc ggtcgaagaa gccaagaaga aacgagcacc ggcagctgcg
     2581 aagaagaaga ccacgacgcg acgcaaagcc tcagcgtcat aaaaggtaaa gcggcccagt
     2641 tggaaacagt ctcgatcaag atccccgcgt cccccgcata catccaggtc gttcggctgg
     2701 tagcggcggg gcttgcatcc agactcaagt tcacgatcga tgagatcgaa gacctcaaga
     2761 tcgccgtgga cgagctgtcc gcctacgtga cgggggctca aggtcgagac ggcaccctcg
     2821 agatcagctt cgccgtgtcc gaggaccgga tcgagatatc cggcgtcggc cggctcgccc
     2881 ccggccagaa agtacgcacc gacctgaccg agttctcgca gatgatcctc gccaccgtgg
     2941 ccgacgaagc ttctctccag cagcccgacg ggaccccggc cttcacgctc gtcaagacga
     3001 ggaaatcgaa cgggtgagcg cgtagtggtc tccgatcgcg cgacccgcgc aactctgact
     3061 cgtga
//
LOCUS       DATMED010000089         6223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_448086, whole genome shotgun sequence.
ACCESSION   DATMED010000089 DATMED010000000
VERSION     DATMED010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448086"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VNP73_02225"
     CDS             <1..796
                     /locus_tag="VNP73_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWL64765.1"
                     /translation="ITTLTRKEMLPLRREMQMIFQDPYSSLNPRQTVGQIIGAPFRIH
                     KTEGDVRKQVQDLMDRVGLNPEHYNRYPHEFSGGQRQRIGVARALALRPKVIVCDEPV
                     SALDVSIQAQILNLLEELQQEFNLTYIFISHDLGVVRHVSDRIAVMYLGRIVELSPAD
                     ELYNHPKHPYTAALLSAVPKGYKDTDVSNRIVLQGDVPSPVDPPPGCPFHPRCPKARI
                     VAGSDDKVPENCRTEMPPLGEVMRDHRAACWYPVEVGDELHRAAHA"
     gene            848..1675
                     /locus_tag="VNP73_02230"
     CDS             848..1675
                     /locus_tag="VNP73_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HWL64766.1"
                     /translation="MTFSIVATDLDASPSPEWGVAVASKFLAVGAVVPWARAGSGAVA
                     TQALANIAYGPDGLELLASGQAAPDVVAALSGVDDMRAQRQLGVVDANGEAATFTGNE
                     CFDWAGGKTGTGYACQGNILTGPEVVHDMAAAFEAADGDLAARMLAALAAGDAAGGDR
                     RGRQSAALMVVRAGGGYGGGTDKTVELRVDDHGAPVEELGRLLDLHRLYFPRPDELDF
                     IPLDDALRTEMQAKLSALGYSGELKKALFAYMGTENLEERWSDDDVIERAVLDHLRR"
     gene            complement(1638..2825)
                     /gene="larC"
                     /locus_tag="VNP73_02235"
     CDS             complement(1638..2825)
                     /gene="larC"
                     /locus_tag="VNP73_02235"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HWL64767.1"
                     /translation="MKVLYFDCFAGAAGDMILGALMDAGAAEDAVRHSIDALDLPANV
                     TFEAVTKNGVRATKARVDASTTTARTLVDITSSLEHSAVPDEVRSLALRIFGRLFEAE
                     ARVHGEDVGRVHLHEAGDDDALVDVVGSSAALLDLRPDLTLSSPLPTGSGWVESSHGR
                     LPVPAPAVLELLKGVPVVERGARELVTPTGAAILAVVVDRFQPMPELTLSAIGYGAGD
                     ADDDFPNVLRVLVGEASDQPTVEEAVLIETNIDDMAPELVPYVIERLLDAGAQDAWTS
                     AIGMKKQRPALQLSVLCDPGDQDRVLDVIYRETTTLGVRLRGIGKHELDRTSIIVQVA
                     GRPVSVKLGLRAGRAVTVSPEFEDAAAAARATGIPLKDVYRRAISAAEALTAEGDPAP
                     RVR"
     gene            complement(2822..3574)
                     /gene="larB"
                     /locus_tag="VNP73_02240"
     CDS             complement(2822..3574)
                     /gene="larB"
                     /locus_tag="VNP73_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWL64768.1"
                     /translation="MRRNEVEELLLAVRDGRVGVDDAVDMLSDLPFSDLGMAMLDTHR
                     ELRQGMPEAVYAEGKTIEQVLEITERLVATTTGPVLVTRATDETGSALVARFPNAVHH
                     RDAGLVVVRSEPRDGVGGLVAVLAAGTSDLPVADEAAFTAAALGLKIERFNDVGVAGL
                     HRLLAVREQLAAADVIVVVAGMEGALASLVGGITPAPVIAVPTSVGYGSSFEGLAALL
                     AMLNSCAAGVAVMNIDNGFGAAMFALRLFQSR"
     gene            3599..4123
                     /locus_tag="VNP73_02245"
     CDS             3599..4123
                     /locus_tag="VNP73_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HWL64769.1"
                     /translation="MPDLNTEQMTLALAIVAGVALVCLLMCLVLAARVRKARREYMVL
                     RGEGGELDLLRAVGRTRKQLDVLNQRVDETLSAHERLEETSRFALQRFHMVRYDAFED
                     MGGRLSFSAALLDDHGNGVVLTSINGRTETRTYAKPVVNLESEHNLSDEERSAIQGAV
                     AGRGRAQTTAAATR"
     gene            4133..5395
                     /gene="serS"
                     /locus_tag="VNP73_02250"
     CDS             4133..5395
                     /gene="serS"
                     /locus_tag="VNP73_02250"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017568146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWL64770.1"
                     /translation="MIDLRLLRDDLPAVEASYARRGGVEGLDRIVELDADHRKILAEI
                     ERLRADQNRASKAIGQASPEDRPAAIADAKKLADAIKELEPQLDSVATELEKLAAALP
                     NLPHESVPQGASEEENAVEREVGDKPSFDFEPKEHDELGEALGLFDQERAAKASGSRF
                     VYLTGRGALLELALVRFTVDFLWDKGFTPIIPPMLVRERAMYGTGFFPADEFEYYRVE
                     RDDLYLAGTSEVPIAAMHSDEIFEGSGLPVRYAGFSTCFRREAGSYGKDTKGLIRVHQ
                     FDKVEQFSFAHPDTSWDEYEVIRANQEAILQALEIPYRVLVMCGGDLGSAAAKKVDNE
                     AWLPGAGRYMEVTSATNATDYQARRLQIRVKEKDGNRYAHTLNGTACAVGRTIVALLE
                     NHQRADGSVEIPEALKPYTGFETLEPVS"
     gene            5437..>6223
                     /locus_tag="VNP73_02255"
     CDS             5437..>6223
                     /locus_tag="VNP73_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HWL64771.1"
                     /translation="MTDYLIAPTGVREVSLDGSRASSDGGFVWSDIESPDGETRTILE
                     RSRGLSSGLLNDALEATLIPKVHVHDRHLLLVTHALDEAGHLMGLHLIVSSDGLVTLH
                     IPLNPTLQKDESVREAEQVLEEVRARPDIASSPGRLALRLMEAVADDLERLLSSAAKR
                     AGQLDRDLREGAGESEEMLDPLFAVRRDIVTVQNRFDQTREAVQTAIEVAPQLLDDID
                     GWRALQTRLMRLSYLCRGERDFFDGVLGLYEQKINVKMNYAMER"
BASE COUNT         1163 a         1980 c         1975 g         1105 t
ORIGIN      
        1 cataacgacc ttgacgcgca aggagatgct gcctttgcgg cgggagatgc agatgatctt
       61 ccaggaccct tactcgtctc tcaacccgcg ccagaccgtc ggtcagatca tcggggctcc
      121 gttccgcatc cacaagaccg agggtgatgt caggaagcag gtccaggacc tgatggatcg
      181 ggtcggtctg aaccccgagc actacaaccg atacccccac gagttctccg gaggccagcg
      241 ccagaggatc ggtgttgctc gagcgcttgc cctacggccg aaggtgatcg tgtgtgacga
      301 gccggtttct gccctcgatg tttctatcca ggctcagatc ctcaaccttc tggaggagct
      361 gcagcaggag ttcaatctga cgtacatctt tatctcgcac gacctgggtg tcgtcaggca
      421 cgtctcggac cggatcgccg tgatgtatct cggccgcatc gtcgagttgt ctccggcgga
      481 cgagctctat aaccatccga agcatcccta cacggcggca ttgctgtcgg ccgtgccgaa
      541 gggttacaag gacaccgatg tgtcgaaccg gatcgtcctt cagggcgacg tgccttcccc
      601 ggtcgaccct ccgccgggat gcccgttcca tcctcgctgc ccgaaggccc ggatcgtggc
      661 aggcagcgac gacaaggtgc cggagaactg ccggaccgag atgccccctc ttggagaggt
      721 catgcgcgac caccgggccg cgtgctggta tccggtcgag gtcggtgacg aactccaccg
      781 agcggctcac gcctaaccct tttgttctaa ctggtggccc atatagggcc ttaaaaggac
      841 atcagaagtg accttctcga tcgtcgcgac cgatctggat gcttccccga gccccgaatg
      901 gggcgtcgcc gtcgcatcga agttcctcgc ggtcggtgca gtggttccct gggctcgagc
      961 cgggtcggga gcggtcgcca cgcaggccct cgccaatatc gcttacggac ccgacggcct
     1021 cgagctactc gcctcggggc aagcggctcc cgacgtcgtc gcggccttgt ccggtgtcga
     1081 cgacatgcgc gcacaacgac aactcggggt cgtcgacgcg aacggggagg ctgcaacgtt
     1141 taccggtaac gagtgtttcg actgggccgg tgggaagacg ggcaccggct acgcgtgtca
     1201 gggcaacatc ctgacgggcc ccgaggtcgt ccacgatatg gctgccgcgt tcgaggctgc
     1261 ggacggggat ctcgccgcgc gcatgctcgc cgctctcgcc gccggtgatg ccgccggagg
     1321 agaccgccgc ggtcgccagt cggcggcgct gatggtggtt cgtgcaggag gcggctacgg
     1381 cggaggaacg gataagacgg tggagttgcg cgtcgacgac catggcgctc ccgtggaaga
     1441 actcggccga ctactagatc ttcaccggct ctacttccca cgaccggacg agctcgactt
     1501 catccctctc gacgacgcgt tgcgcaccga gatgcaggcg aagctgtccg cgctcggtta
     1561 cagcggggag ctcaagaagg ctttgttcgc gtatatggga acggaaaatc tagaggagcg
     1621 gtggtccgac gacgacgtca tcgaacgcgc ggtgctggat caccttcggc ggtaagcgcc
     1681 tccgcggcag agatcgccct ccgatagaca tccttcaaag ggatccccgt cgcgcgcgca
     1741 gctgcggccg cgtcctcgaa ttcgggtgag acggtcactg ctcgccccgc ccgcaaaccg
     1801 agctttacgg agacagggcg tcccgcgacc tggacgatga tcgaggtccg gtcgagttcg
     1861 tgtttgccga tgccgcgcaa gcggaccccg agcgtcgtgg tctcgcggta gatcacatcg
     1921 agcacccggt cctggtctcc cggatcgcag aggaccgata gctgtagggc cggcctctgt
     1981 ttcttcatgc cgatggcgga ggtccacgcg tcctgggcgc cggcatccag caagcgctcg
     2041 atcacgtagg gcaccagctc cggggccatg tcgtcgatgt tcgtttcgat cagcacggcc
     2101 tcttcgaccg tcggttgatc gctcgcttca ccaacgagca cgcgcaggac gttggggaag
     2161 tcgtcgtcgg catcgcccgc gccgtagccg atcgcggaca aagtcagctc cggcatcggc
     2221 tggaaccggt ctacgaccac cgcgaggatg gcagcccccg tcggcgttac caactcgcgc
     2281 gcgccccgct cgacgaccgg aacgcccttc agaagttcga gcacggcggg ggcggggaca
     2341 gggagcctcc cgtgactcga ttccacccag ccggatccgg taggtagcgg tgagctcaga
     2401 gtgagatccg gcctcagatc caggagcgcc gcgctgcttc caacgacgtc tacgagagcg
     2461 tcatcatctc ctgcctcatg gaggtgcacc cgacccacgt cctctccgtg aacccgtgcc
     2521 tcagcctcga acaaacgtcc gaagatcctg agtgcgagag agcgcacttc gtcgggcacg
     2581 gcggagtgtt caagcgacga agtgatgtcg acgagtgtcc gtgctgttgt tgtcgacgcg
     2641 tcgacccgcg ccttggtcgc gcgcacacca ttcttggtca cagcttcgaa cgtgacgttc
     2701 gcgggcagat ccagagcgtc gatgctgtgc cggaccgcgt cctcggccgc ccccgcgtcc
     2761 atcagcgcac cgaggatcat gtcccctgcc gcccctgcga agcaatcgaa gtagaggacc
     2821 ttcatcgact ctggaagagt ctcagcgcga acatcgcggc gccgaaaccg ttgtcgatgt
     2881 tcattacggc cacgccggcg gcacacgaat tcagcatcgc cagtagggcg gcgagacctt
     2941 cgaacgagga cccgtaaccc acgctggtcg gaacggcgat tacgggggcc ggggtgatcc
     3001 ctccgacaag tgaagcgagc gcgccctcca tcccggccac gacgacgatc acatcggccg
     3061 ccgccagttg ctcgcgcaca gcgagtaatc ggtgaagacc ggccactccg acatcgttga
     3121 aacgttcgat cttcagtccc agcgcagcgg ccgtgaatgc agcttcatcc gcgaccggga
     3181 gatcggaggt gcctgcggcg agaacggcga cgagaccgcc gacaccgtcc ctgggttcgg
     3241 accgaaccac gacgagaccg gcgtcgcggt gatgaaccgc gttcgggaag cgggcgacaa
     3301 gagccgatcc cgtctcgtcg gttgccctgg tgaccaggac gggccccgtt gtggttgcca
     3361 cgagccgctc cgttatctcg agaacctgtt cgatcgtctt gccctcggcg taaacggcct
     3421 ccggcatccc ttgccgcaac tcgcgatggg tgtcgagcat cgccatgccg aggtcgctga
     3481 agggaagatc gctgagcatg tccacggcgt cgtcgacacc gacccggccg tcgcgcacag
     3541 cgagaagtag ttcctctacc tcgttccggc gcatacctcc agtgtaggat gcgccaccat
     3601 gccggacctc aacaccgagc aaatgacact cgcgctcgcc atcgtggcgg gcgttgctct
     3661 tgtgtgccta ctgatgtgcc tagtcctcgc agcaagggtg aggaaagcgc gccgcgagta
     3721 catggttctc cgcggcgaag gcggcgagct cgacctcctt cgggccgtcg gccgtacccg
     3781 taaacagctc gacgtgctca atcagcgagt cgacgagacc ctcagcgcgc acgaacggct
     3841 ggaagagacg agtcgcttcg ctctgcagcg cttccacatg gttcgttacg acgctttcga
     3901 ggacatgggc ggtcgcctct cgttctcggc cgctttgttg gacgatcacg gcaacggggt
     3961 cgttctcacg tcgatcaacg gtaggaccga gaccaggact tacgccaaac ctgtggtcaa
     4021 cctcgagtcc gagcacaacc tctccgacga ggagcgctcc gcgatccaag gagcggttgc
     4081 ggggcggggc cgagcgcaga cgaccgccgc cgcaacgcgg taaggacggg ccgtgatcga
     4141 ccttcgctta ctgcgggacg acctccccgc agtcgaggcg tcctacgcac gcagaggtgg
     4201 tgtcgaagga ctcgatcgca tcgtcgaact cgatgcggat catcggaaga tcctcgccga
     4261 gatcgagcgc ttgcgggcgg atcagaatcg agcgtcgaag gcgatcgggc aagcttcgcc
     4321 cgaagatcgt cctgcagcga tcgccgacgc gaagaagctc gccgacgcga tcaaggagct
     4381 cgagccccaa ctggattccg tcgcgaccga actagagaag ctcgcggcgg cgttgccgaa
     4441 cctccctcac gagtccgttc cccagggtgc atccgaggaa gagaacgccg ttgaacgtga
     4501 ggtcggcgat aagccctcgt tcgacttcga gcccaaggaa cacgacgaac tcggtgaagc
     4561 actcggattg ttcgatcagg aacgtgcagc gaaggcatcc ggcagcaggt tcgtctatct
     4621 aaccggccgc ggtgctctgc tcgagcttgc tcttgtcagg ttcaccgtcg acttcctatg
     4681 ggacaaggga ttcactccga tcatcccgcc gatgctcgtg cgcgaacgcg cgatgtatgg
     4741 gaccggtttc ttcccggccg acgagttcga gtactacagg gtcgagagag acgatctcta
     4801 tctcgcaggt acctccgagg tcccgatcgc ggcgatgcac tcggacgaga tcttcgaggg
     4861 atccggcctt ccggtcaggt acgcaggctt ctccacgtgc ttccgcagag aggccggttc
     4921 ttacggcaag gacacgaagg gactgatcag ggtccaccag ttcgacaagg tcgagcagtt
     4981 ctcgttcgca cacccagaca catcgtggga cgaatatgag gtgatccgcg cgaaccaaga
     5041 ggccatcctg caggcgctgg agatccccta ccgcgtgctc gtgatgtgcg gtggggatct
     5101 gggctcagcg gcggccaaga aggtcgacaa cgaagcgtgg ctcccggggg ccgggcggta
     5161 catggaagtg acgtcggcta cgaacgcgac ggactaccag gcccgtcgcc tgcagatccg
     5221 cgtgaaggag aaagacggca accgctacgc gcacacactg aacggcacgg cctgcgctgt
     5281 aggtaggacg atcgtcgccc tgctcgagaa ccaccaacgt gccgacggaa gcgttgagat
     5341 ccccgaagct ctcaagccct acacggggtt cgaaacgctc gaacccgttt cctagaccgc
     5401 cttgcggcgg ttcgaccgtc gcttcatcct tattgtgtga ccgattacct gatcgcgccc
     5461 accggcgtgc gtgaggtttc gctcgatggg tcaagagcgt cctctgacgg tgggttcgtg
     5521 tggtcggaca tcgagtcacc cgacggtgag acgaggacga tcctcgaacg ctctcgtggc
     5581 ttgagcagcg gtctattgaa cgatgcgctc gaagcgacgc tgatcccgaa ggtccacgtt
     5641 cacgaccggc acctgttgct cgtgacgcac gctctggacg aagccgggca tctgatgggg
     5701 ctgcacctca tcgtgtcctc cgacggactt gtcacgttgc acatcccgct caaccccacc
     5761 ttgcagaagg acgagagtgt gagggaggcg gagcaggtcc tggaagaggt gcgtgcacgc
     5821 cctgatatcg catcgtctcc gggacgtctt gctcttcgtc tgatggaagc cgtcgccgat
     5881 gacctcgaac gtctgctgtc gtcggccgcg aaacgggccg gccaactgga ccgagatctg
     5941 cgcgaggggg ccggcgagag cgaagagatg ctcgatccct tgttcgccgt tcggcgcgac
     6001 atagtcacag tgcagaaccg tttcgatcag acccgcgagg ccgttcaaac ggctatcgaa
     6061 gttgcaccgc aactcctcga cgacatcgac gggtggcggg cgttgcagac gcgactcatg
     6121 cggctgtcat atctgtgtag aggagagcgt gatttcttcg acggagtcct cggcctctat
     6181 gagcagaaga tcaacgtgaa gatgaactac gcgatggagc gtc
//
LOCUS       DATMED010000090         4406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1211816, whole genome shotgun sequence.
ACCESSION   DATMED010000090 DATMED010000000
VERSION     DATMED010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211816"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1112)
                     /locus_tag="VNP73_02260"
     CDS             complement(162..1112)
                     /locus_tag="VNP73_02260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL64772.1"
                     /translation="MSSKRIQMDAGEFHAAVNEVFHAFEAPVYDEVHQDMWRSLPRQF
                     ELLANELVDNDSETIVPQISVLDVGCGTGLATDLLLRSPLGKFVNEVTLLDPSTEMLE
                     LAMHRSRDWKIDVQPRVGVLCDLPEDATYEFVIACSVLHHIPDLAAFCGELTRITPTG
                     GHLLHIHDPNGDISQDREWEERALRLKRSLAPRTSKDRFSLPHIKGKFWSMFPWLRKK
                     SYLDRVNEELRRRGLISSNLSSEEIWRITDLRVYDDIGIQISHLKDLLAGFRLINHRS
                     YGFFGRLWSDLPKELRPEEERMIEDGRKDGAFIGALWQRD"
     gene            complement(1155..1838)
                     /locus_tag="VNP73_02265"
     CDS             complement(1155..1838)
                     /locus_tag="VNP73_02265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010496240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWL64773.1"
                     /translation="MRAGSLLGLLRAPGIALRSATLSYRYRPAELLKLPIRAVWTARL
                     DKHPDAGLSLGGRLYLGFFPGPDGIRSSEEIVHLARDRPVRVTLSRGSTFETQGVVLV
                     APGAEFVVSENGELSIGSGVLINSGVSVLCWDSVTIGDDCSFAFDSVVMDTDFHRIRP
                     GQAGVTAPVVIGNHVWVGARATILKGVTIGDGAIVAAGSIVTHDVPARALVAGVPAGV
                     IRHDVEWDR"
     gene            1903..4059
                     /locus_tag="VNP73_02270"
     CDS             1903..4059
                     /locus_tag="VNP73_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64774.1"
                     /translation="MGTVAGIEQILALIGRKTRVIHGSSEASGYGGGGDIDCVVEGLD
                     GLWPLRLQSPARLVNRLRYDVTATSWFVESNGEGVSIDTLEDPDGIGKYGFSTGSVTG
                     ESLVDPGSAAAYLAIKRLRKRNLEAAAWVQVAKLATHDADGFRKKLSASLPRTAEAVE
                     AVVLASTAPDRALAARALRDLRITRLHHPFRIARYTWLGAGRIAERLTYSTGLYVVLV
                     GPDGTGKSTIAHALAADCFGFRRSRRLHWRPGILPGSRTGGPGGRDTTRPHDTKGRSR
                     GRSVPIVLYHWADFLIGSLATIWPATRRSTLIVAERPWWDLGVDPLRYRLDVSPELVS
                     KLGRILRKPDLVLNLAAPAATLVARKPEITLEATDRQLASWQTLAPALTRTESIDVTG
                     TPEQSIAKVRSAIHAVAADQAFRNLGPGWVAVPNRRRPRWWVPRGPRLVARATFDSWT
                     PMTRSGSSRWRAAGALAGFGGLRALPRSTPPRDVIERVAPHLERGENVAIHRTNHRGR
                     FIVLFVSPQGAVRRFAKVVTDGDAEPLDREAAALQNLALTLPAPLRAPELLERGEGVL
                     VLEAVSRRRDWDLRIESDVAQALGTFAGGGGTHGDFAPWNLIPADGWVLVDWEEANTD
                     GTQFEDMFHYLVQSCVLLGSPSEPEIIDGVLHERGWIGTALRAFSDASGHDLNEVGSA
                     MTRYLEDSSLRLDRKAPGARAGLEVRARLARALEGR"
     gene            4056..>4406
                     /locus_tag="VNP73_02275"
     CDS             4056..>4406
                     /locus_tag="VNP73_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWL64775.1"
                     /translation="MRIVVFAYACEPGKGSEPGAGWALTQMIASFADVHVITRSNNQA
                     RVMSAGTHFPGGDRLTFSFVERPAWARRFKKGQRGIRIYHSLWQLRALKEARHLHERV
                     PFDAAWHITIANAWL"
BASE COUNT          847 a         1403 c         1342 g          814 t
ORIGIN      
        1 acgagatgcg gctacgccca cacccgtggg agttctacct gtactacgac atctaaggag
       61 acgtaggcag tcggtccgtc gaacgccggc gggctcggcc gtcggcgaaa acaaccacga
      121 ggcccccggg aaaccggggg cctctatctt ctttagggat atcaatcgcg ctgccagagc
      181 gcccctatga aagctccatc cttcctgccg tcctcgatca tcctttcttc ttctgggcgg
      241 agctctttcg gtaggtcgct ccacaggcgt ccgaagaatc catacgaccg atgattgata
      301 agcctgaacc cggccagaag gtccttcagg tgagaaatct ggatcccgat gtcgtcgtaa
      361 acgcgcagat ccgtgatgcg ccagatctct tctgaggaca ggttcgatga gatcagaccg
      421 cgacgacgaa gttcttcgtt cacccgatcc agatagctct tcttcctgag ccacgggaac
      481 atcgaccaaa actttccctt tatgtgaggg aggctgaagc gatccttgct cgttctaggg
      541 gcaagcgatc tcttcaaccg aagggctcgc tcctcccatt ccctgtcttg cgagatgtcg
      601 ccgttgggat cgtggatgtg aaggaggtga ccgccggtcg gggtgattcg ggtcagctcc
      661 ccacagaaag cggctaagtc ggggatgtga tgcagaacgg aacacgcgat aacgaactcg
      721 taggtagcgt cctccggtag atcacacagc actccaaccc ggggttggac atcgatcttc
      781 cagtctcgac tccgatgcat cgcgagttcc aacatctctg tcgatggatc caagagagtg
      841 acttcattca cgaacttgcc tagaggggac cgcaagagga gatccgttgc gaggcccgtt
      901 ccgcatccca cgtcgagtac cgagatctgg ggaacgatcg tctcggagtc gttatccacc
      961 agctcgttcg ccagcaattc gaattgccta ggaaggctcc gccacatgtc ttggtggacc
     1021 tcgtcgtaaa cgggcgcctc gaatgcatgg aatacctcgt tgaccgcggc gtggaactcg
     1081 ccggcgtcca tctggatccg cttgctcgac atgagcgatc ggataggccc gaagggatcc
     1141 tcggttgcca cctgttatct atcccattcg acgtcgtgcc tgatcacgcc ggcaggaacc
     1201 ccggcgacca atgcacgcgc gggcacatcg tgggtcacaa tcgatcccgc tgccacgatc
     1261 gcgccgtcgc cgatggtgac ccccttgagg atcgtcgccc ttgcaccaac ccagacatgg
     1321 ttaccgatca cgacaggcgc ggtgacaccg gcctgccccg gacggatccg gtgaaagtcg
     1381 gtgtccatga cgaccgaatc gaacgcgaag ctgcaatcgt cgcctatcgt gacgctgtcc
     1441 caacagagca cgctgactcc ggagttgatc aacacgcccg acccgatgct gagttctccg
     1501 ttctcggaga ccacgaactc ggcccccgga gcaaccagaa caactccttg cgtttcgaat
     1561 gtgcttccgc gactcagtgt tacgcgcaca ggtcgatcgc gagcgagatg cacgatctct
     1621 tcggaactgc ggatcccgtc cgggccgggg aagaacccca gatagagccg gcccccgagc
     1681 gacagccccg catcgggatg cttgtcgaga cgggccgtcc acactgcgcg gatcgggagt
     1741 ttgagtaact cggcggggcg gtatcggtac gaaagcgtcg cgctgcgcag tgctatgccc
     1801 ggggcccgca acaatcccag aaggctgccc gctctcatct ccagatagtc ccacggagat
     1861 ctcaaaagag ggccgtccgc gaacctgcgg catcctatgg agatgggaac ggttgccggg
     1921 atcgagcaga tcctcgccct gatcggtcgg aagacccgcg tgatccacgg gtcctccgaa
     1981 gcatcgggat acggaggcgg aggagacatc gattgcgtcg tcgaaggcct agatggcttg
     2041 tggccgctcc gactgcaatc tcccgcccgc ctagtgaatc ggcttcgcta cgacgtaacg
     2101 gcaacgtcct ggttcgtgga atcgaacggc gagggcgtat ccatcgacac gcttgaagat
     2161 cccgatggga tcggcaagta cggtttctcg actggaagtg tcacgggtga aagtctcgtc
     2221 gatccgggct ccgcggccgc ttacctcgct atcaagcgtc tgcgaaagag aaacctcgag
     2281 gctgcagctt gggtacaggt cgccaaactc gccacccacg acgccgacgg cttcagaaaa
     2341 aaactgtcgg cttccctgcc ccgtacggct gaggcggtcg aggcggtcgt tctagcaagc
     2401 accgcacccg accgcgcatt ggctgcacgg gctctgcggg acctacgcat aaccagactt
     2461 caccatccgt tcaggatcgc tcgttacacg tggctcggag ccggacggat agccgaaagg
     2521 ttgacctact cgaccggcct ctacgtggtg ctcgtggggc cggacggaac cggcaaatca
     2581 acgatcgccc acgctctcgc cgcggactgc tttgggtttc gtcggtctcg cagactgcac
     2641 tggcggccgg gcatcttgcc cggctcacga acgggcggac ccggtgggag agatacaact
     2701 cgcccccacg acaccaaggg gcggtcgcgg ggccgctcgg ttccgatcgt tctctatcac
     2761 tgggccgact tcctcattgg ttcacttgca acgatctggc cggcgacgcg tcgatcgacc
     2821 ctgatcgtcg ccgagcgacc atggtgggac ctgggtgtcg atcccctccg ctaccggctc
     2881 gacgtatccc ccgaactcgt atcgaaactg ggccgcatac tgcggaaacc agacctcgtc
     2941 ctcaacctcg ctgctccggc cgccaccctc gtagcccgca aaccggagat cacgctggag
     3001 gccaccgacc gccagctggc atcgtggcag acactcgccc ccgcgctcac tcggaccgaa
     3061 tcgatcgacg tgaccggtac cccggaacaa tcgatcgcaa aggtccgaag tgcgatccat
     3121 gccgtcgcgg ccgatcaagc cttcaggaat ctagggccgg gttgggtggc ggtgccgaac
     3181 cgtcgccgcc ctcgatggtg ggtaccccgg gggccgcggc tcgttgcgcg cgccacattc
     3241 gattcgtgga cgccgatgac ccgatccggc tcctctcgct ggcgcgccgc gggagcgctt
     3301 gcgggcttcg gcgggcttcg cgccttgccg cgttcgacgc cccctcgaga cgtaatcgag
     3361 cgggtggcgc cgcatctcga gcgtggcgag aatgttgcga tccaccgaac gaaccaccgc
     3421 ggccgtttca tcgtcctgtt cgtctcgcct cagggcgccg tacgccgatt cgccaaggtc
     3481 gtcacggacg gagacgcgga accactcgat cgcgaggccg ctgcgctaca gaaccttgcc
     3541 ctcactctcc ccgcgccgtt gcgcgctccg gaacttttgg agcgaggcga aggagtcctc
     3601 gtgctggaag ccgtctcgcg tcgccgagat tgggacctgc gcatcgagtc cgacgtggcg
     3661 caggcgctgg gaacgttcgc cggcggcgga ggaacgcacg gcgacttcgc cccatggaac
     3721 ctgatcccgg ccgacggctg ggtactggtc gattgggaag aggcgaatac ggatgggacg
     3781 cagttcgagg acatgttcca ttacctagtt cagagctgcg tccttcttgg atcgccgagc
     3841 gagccggaga tcatcgacgg cgtccttcac gaacgaggat ggatcgggac tgcactgcgc
     3901 gctttctcgg atgcgagtgg acacgatcta aacgaggtcg gttccgctat gacgcgctat
     3961 ctcgaagaca gctcgttacg cctcgatcgc aaagcaccag gagctcgggc cggcctcgaa
     4021 gttcgagcac gtctcgcccg tgcgctggaa ggtcgatgag gatcgtcgtc tttgcctatg
     4081 cgtgcgagcc cggcaagggg tcggagcccg gagccggttg ggcgctgact cagatgatcg
     4141 cgtcgttcgc cgatgtccac gtgatcacga ggagcaacaa ccaagcacgg gtcatgtcgg
     4201 caggcactca cttcccgggc ggagaccggt tgaccttcag cttcgtcgaa cgaccggctt
     4261 gggcacgtcg cttcaagaag ggacaaagag ggatccggat ctatcactcg ttgtggcaac
     4321 tgagagcctt gaaggaagcc cgacatcttc acgagcgggt cccattcgat gcggcgtggc
     4381 acatcaccat cgcgaacgcg tggctg
//
LOCUS       DATMED010000091         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3907865, whole genome shotgun sequence.
ACCESSION   DATMED010000091 DATMED010000000
VERSION     DATMED010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907865"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VNP73_02280"
     CDS             <1..241
                     /locus_tag="VNP73_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWL64776.1"
                     /translation="NPAPGAWSQLTGKRLKVFRVEAEATADLTPGAITAGRTLLVGTG
                     DGPVALEEVQLEGKRRMSGAELARGLRIEPGTRLE"
     gene            238..912
                     /gene="rpe"
                     /locus_tag="VNP73_02285"
     CDS             238..912
                     /gene="rpe"
                     /locus_tag="VNP73_02285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWL64777.1"
                     /translation="MSRLQLAPSILTADFGRLADEVRSVADHVDWFHLDVMDGHYVPN
                     LTFGPSTVAAIKRSCDRPLHVHLMIEEPERYAATFVAAGAARISFHPEVVDDVAGLIA
                     EIRSLGAGAGLAIHPDVPVDVVESHALDLDVVLMMSVRPGFGGQAYLHEVTPKITEAK
                     RLVVTTGSSADIEVDGGVNLSTVEEAVSAGGEILVAGSAVFDGQDPVAAARGMRSKLD
                     ELGNSK"
     gene            909..1844
                     /locus_tag="VNP73_02290"
     CDS             909..1844
                     /locus_tag="VNP73_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020113535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL64778.1"
                     /translation="MSRATELILIADDDDDIARFIEVNLVLEGYEIAIASDGEQTLHT
                     AYDRRPDLILLDIMMPKIDGYEVCQRLRNDPRTRNISIIMLTAKSMSADRVVGLTAGA
                     DDYMIKPFDPIELVARVKTALRRSAEMRSVNPLTQLPGNVQVQEQVSQHVASNRPFAV
                     MYIDLDNFKAFNDYYGFLRGDEAIKLLARCAGEAVSDRGGFLGHVGGDDFIGIVTPEV
                     SESVALKIIDRWDTKIVALYDREDLDRGYIEVVDRQKNQHRYPITTVSIGIASNTEKP
                     IETHWEASEIASEMKSFAKRERRSSYAIDRRRSEP"
     gene            1882..2955
                     /gene="ribD"
                     /locus_tag="VNP73_02295"
     CDS             1882..2955
                     /gene="ribD"
                     /locus_tag="VNP73_02295"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWL64779.1"
                     /translation="MHEDFMRRAIRLARVPAATSPNPRVGAVVVRDDEVVGEGYHLGP
                     GTPHAERAALDAAGSAARGADLFVNLEPCAHHAHTPPCADAVIEAGVRRVFASLEDPD
                     DRVRGRGLARLREAGIEVEVGLLEREAAEANRAFLHQRTTGKPLVTLKMALSIDGRMA
                     AADGSSRWITGEEARRFAHERRAEVDAILIGAGTAVADNPRLTARGIPDARQPARVLI
                     DGSGRVEASAQLFDEGEAIVVTTARCAHDRQIEWKEAGAEVLVLPDRDGHVVLGAVIE
                     TLAERGWIEILCEGGPSLSSALLASEVVDRLEIHRGSIVLGSGPGIGDVGVGTIGDAM
                     GWDLADTFPVGSDVVSVYDRGTP"
     gene            2956..3564
                     /locus_tag="VNP73_02300"
     CDS             2956..3564
                     /locus_tag="VNP73_02300"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWL64780.1"
                     /translation="MFTGIVVERGSVKKAAQRRGLLELEIEAPHIARELEEGDSVAVN
                     GVCLTAVGTSRKRFSTQAMPETVARSTLGALRRGSKVNLELPARLSDRLGGHLVQGHV
                     DATARVIRSENDSEARRIWFTCGADVLRYLVDKGSITLDGVSLTVVEVGETTFQVALI
                     PHTLDVTTLGTLEVGSSVNVEVDVIAKYVERLANGHFRFAAD"
BASE COUNT          692 a         1067 c         1195 g          623 t
ORIGIN      
        1 caacccggct ccgggggcgt ggtcccagct gaccggtaag cggttgaagg tattccgagt
       61 cgaagccgag gcaacggccg acctcactcc cggggccatc acggccgggc ggaccctgct
      121 ggtcgggacg ggggacgggc cggtcgcgct ggaagaggta caacttgaag gcaagcggag
      181 gatgtcgggg gccgagctgg cccgaggtct aaggatcgaa ccgggaacga ggctcgagtg
      241 agcaggctgc agctcgcacc ttcgatactg acggcggact tcggccggtt ggccgacgaa
      301 gtgcggagcg tcgccgacca cgtcgactgg ttccacctcg acgtgatgga cggccactac
      361 gtacccaatc tgactttcgg cccgtccaca gtggcggcca tcaaacgctc ctgcgaccga
      421 ccattgcatg tccatctcat gatcgaagag cccgaacgct atgcagccac attcgtggct
      481 gcaggagccg cgcggatctc cttccaccca gaggtcgtcg acgacgtggc cggtttgatc
      541 gccgagatca ggtctctggg ggccggagcc ggcctcgcga tccatccgga tgtccccgtc
      601 gatgtcgtgg agtcacacgc tctcgacctg gacgttgtcc tgatgatgtc ggtgcggccc
      661 ggattcggcg gccaggccta cctccacgag gtaaccccga agatcacgga agcgaagcgc
      721 ctggtagtga caacggggtc gtctgccgat atagaggtcg acggtggggt aaacctctcc
      781 accgtggaag aggcggtttc cgccgggggg gagattctcg tggcaggcag tgcggttttc
      841 gatggccagg atcccgttgc cgccgcgcgt gggatgagat cgaaactcga cgagctcgga
      901 aactcgaagt gagccgcgcc accgaactga tcctgatcgc agatgacgac gacgacatcg
      961 ccagattcat cgaggtcaac ctcgtgctcg aaggctacga gatagcgatc gcttccgacg
     1021 gagaacaaac acttcacacc gcttacgacc gccgtccgga tctgatcttg ctcgacatca
     1081 tgatgccgaa gatcgatggt tacgaggttt gccagcgctt gcgcaacgac ccgaggacca
     1141 gaaacatctc gataatcatg ctcactgcaa agtccatgtc ggccgaccga gtcgtcggcc
     1201 tgaccgcagg tgccgacgac tacatgatca agccgttcga tccgatagag ctggtcgcgc
     1261 gcgtcaagac ggccttgcgt cgatcggccg agatgcgctc ggtcaatccg ctcacgcagt
     1321 tgccggggaa cgtgcaggtt caggaacagg tcagccaaca cgttgcaagt aatcgcccgt
     1381 tcgcggtcat gtatatcgac ctcgacaact tcaaggcctt caacgactac tacgggttcc
     1441 tgagaggcga tgaagcgatc aagctactgg ctcgctgcgc gggcgaggcg gtgtccgata
     1501 ggggagggtt cctgggacac gtcggcggcg acgacttcat cgggatcgtg acaccggagg
     1561 tgtccgagag cgtggcgctc aagatcatcg accgctggga tacgaagatc gtcgctctct
     1621 acgaccgcga ggacctcgac cgcggttaca tcgaagtcgt ggaccgccag aagaatcagc
     1681 atcgctatcc gataacgacc gtgtcgatcg gcatcgcaag caataccgag aagccgatcg
     1741 agacgcactg ggaggcctcg gagatcgcat ccgagatgaa atcgttcgcg aagcgggagc
     1801 gccgctccag ctacgcgatc gaccgccgaa ggtcggagcc ctagcggaaa gcgactagag
     1861 ttgctccact caggaggaag cgtgcacgaa gacttcatgc ggcgggccat ccggctagcg
     1921 cgggtgcccg cagccacctc tcccaatccc cgcgtcggcg cggtcgtcgt tcgcgatgac
     1981 gaggtcgtag gggagggata tcacctcggc cccgggactc ctcacgcaga gcgtgccgca
     2041 ttggatgctg cgggatcggc agcccgcgga gccgacctct tcgtcaacct cgaaccatgt
     2101 gctcaccatg cccacacacc cccttgtgcc gacgcggtga tcgaggccgg cgtccgccgg
     2161 gtcttcgcct ctctggagga tccagacgac cgtgtgcggg gtcgtgggct cgctcgtctg
     2221 cgagaggcgg gcattgaggt cgaagttggg ttgttggagc gagaagcagc cgaggcgaat
     2281 cgagcgttcc tgcaccagcg caccacgggc aaaccgctgg tgacgctcaa gatggcgctg
     2341 tcgatcgacg gtcgaatggc ggctgccgac ggcagctcgc ggtggatcac cggcgaggag
     2401 gcccgtcggt tcgcgcacga acgccgagcc gaagtcgatg ccatcttgat cggagcgggc
     2461 acggcagtcg ccgacaaccc gcggctgacg gcacgcggta tccccgatgc gcgtcaaccc
     2521 gcacgggtcc tgatcgatgg gtcgggtcga gtagaagcct cagcgcagtt gttcgatgag
     2581 ggcgaggcga tcgtcgtgac gacggcgcga tgcgcacacg acagacagat cgagtggaag
     2641 gaagcagggg ccgaggttct cgttcttccg gaccgcgatg gacacgttgt tctcggagcc
     2701 gtgatcgaga cgctcgccga gcgaggatgg attgagatcc tgtgcgaagg cggtccctct
     2761 ctgagtagcg ctcttcttgc atccgaggtg gtcgatcgtc tcgagatcca cagaggttcg
     2821 atcgtgctcg gttccgggcc ggggatcggc gatgtggggg tcggcacgat cggcgacgca
     2881 atgggttggg acctggcgga tacgttcccg gtcggcagcg acgttgtcag tgtctacgac
     2941 aggggaactc cttagatgtt cacgggcata gtcgtcgaga gaggttcggt caaaaaggcg
     3001 gcccagcgcc gcggtctcct cgagttggag atcgaagctc ctcacatcgc gagggagctc
     3061 gaggagggtg actcggtagc cgttaacggc gtttgcctga ctgccgtggg tacatcgcgg
     3121 aagcggttct ctacacaggc catgcccgag accgtggcca gatcgacgtt gggggccctc
     3181 cgcaggggaa gcaaagtgaa cctcgagctg ccggctcgat tgtcggatcg cctcggaggg
     3241 catctcgtcc agggacacgt cgacgccacc gcccgggtca tccgatccga gaacgattcc
     3301 gaagcgagaa ggatctggtt cacctgcgga gcggacgtgc ttcgctacct cgtcgataaa
     3361 gggtcgatca cgctggacgg cgtatccctt acggttgtgg aggtcggtga gacgacgttc
     3421 caggtagcgc tgatcccaca caccctcgac gtaaccaccc tcggcaccct cgaggtcggg
     3481 tcgagcgtga atgtcgaggt ggatgtcatc gcaaagtacg ttgaacgcct ggcgaacggg
     3541 cacttccggt tcgccgccga ttaggaggcc atgttgc
//
LOCUS       DATMED010000092         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_839907, whole genome shotgun sequence.
ACCESSION   DATMED010000092 DATMED010000000
VERSION     DATMED010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839907"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            602..2035
                     /gene="proS"
                     /locus_tag="VNP73_02305"
     CDS             602..2035
                     /gene="proS"
                     /locus_tag="VNP73_02305"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWL64781.1"
                     /translation="MAKGLPKQSEDFPGWYVAVVREADLAEHGLARGSQVIKPYGYTI
                     WENIQRALDDRFKATGHENFMFPVLIPESVIAKEKDHVEGFSHEFWTVTQVGGQELEE
                     KLILRPTSEAAIWNAYANWIQSYRDLPLLYNQWCNVFRYELRTRLFLRTSEFLWQEGH
                     TAHATAEEAWDEAVQMLHVYREVAEDVLAIPVLWGRKSAAERFAGADETLPIEALMRD
                     AKALQCGTSHYLGQNFAKAYDVTFLNKEGEQEHAYGTSWGFSSRMVGGTVMSHGDDDG
                     LRLPPAVAPYQVVVVPIYRSDDERASVLEVVAKIRDELAGNGVRVRVDDRDQHRPGFK
                     FAQWELKGVPIRIEIGPKDVDAGQVVVVDRVTREKKALPTSEALSSMVGALESVQKSL
                     FEDALKFREANTHEIESFDKFKEGVEAEGGFWVGAWCGAADCEARIAGETKTSIRVLP
                     LELEDPGAPCMHCGKPGVERATWARAY"
     gene            2147..>3197
                     /locus_tag="VNP73_02310"
     CDS             2147..>3197
                     /locus_tag="VNP73_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL64782.1"
                     /translation="MTSLVRKLSVALIGGLLLAGLLPASPAAADPTGFVIMSDGVEIA
                     VNVRMPDNFEEGKSYPTVFEMNGYDGGSSDGDEPEPANEGSRVLTKQFYDDYVTVHAS
                     VRGTGCSGGEFDLFSWRSALDGREVIEWIADQEWSNGDIGIYGHSYGGITGFMVASTR
                     PPELDAVSVSGLIDDLYRGIVYPGGVSNYGFPLLWTGGVRNVYDVGGGLFYGMWEEDG
                     SVQPGCVTTAPTKSRTVLNDAILQGLQETDSTWMQARSMINYAERINVPIHISGAYQD
                     EQTGPRGPYHLFEEVVNAPVRRLLMTNGDHGTQQDPFVMADRVKWMDHWVRNVSGGYG
                     PLTEDRSSVTTFLDMR"
BASE COUNT          604 a         1007 c         1023 g          563 t
ORIGIN      
        1 gcggcaacgc actcgtaatc cgctttttgc aggatccgta cgagcatcat ccgttggctg
       61 agatcgtcat caacgactaa gacacgattg gcgccgggat cctccgggat cacgctggac
      121 atgcctcaca ttgttaaccc atagatgaag ggtgatgtcg gcaagaagac cggcgagacc
      181 cgagccgcct tcagggccgc tcggtagccg gagggccggt tattggagcg ttgtcctcca
      241 gccgctcggg tctccccatg tcctactcca gtcggtcgcc ggaggcaccc ggctccggtc
      301 tcccggcgct ctcgagatgc gaacccggcc gaaaccgagt cgcttcccaa ggcgggccta
      361 ggggaccttt tcttggatcc gagaacctcg ttcccgtgcg accgtgcaga agctcatcga
      421 ccataggaac ctcggcggtc gcatgtcaac gaaataaccg tggaatgcga aagaacgcag
      481 ttctgaacac cttgtccaca ttcgtgtacg cctccatcca caggtgttgt ggaaaaccct
      541 ccgggctcga ccgcttttcg ctctgcgcgc tcgccgaggc gcccgcacgt actcttccca
      601 catggcgaag gggcttccga aacaatctga agactttccc ggctggtatg tcgctgtggt
      661 tcgcgaggcg gacctggccg agcatggtct cgcacgcggc tcccaggtga tcaagcccta
      721 cggctacacg atctgggaga acatccagcg tgccctcgac gaccgcttca aggcgaccgg
      781 gcacgagaac ttcatgttcc ccgtgctcat acccgaatcg gtgatcgcca aggagaagga
      841 ccacgtcgaa ggcttctcgc acgagttctg gaccgtcacg caggttggcg gtcaagaact
      901 cgaggagaag ctgatcctta ggccgacgtc ggaagccgcc atctggaacg cgtacgcgaa
      961 ctggatccag agctaccgcg acctgccgct cctctacaac cagtggtgca acgtgttccg
     1021 ctacgagcta cgcacccggc tgttcctccg gaccagcgag ttcctgtggc aagaggggca
     1081 cacggcgcac gcaactgcgg aggaggcatg ggacgaggcc gtgcagatgc ttcacgtcta
     1141 tcgggaggtc gctgaagacg tgctcgccat ccccgtcctg tgggggcgga agagcgccgc
     1201 tgagcgcttc gctggggccg acgaaacgct tccgatcgaa gctctcatgc gtgacgccaa
     1261 ggccctgcaa tgtgggacgt cgcactacct cggtcagaac ttcgccaagg cctacgacgt
     1321 gactttcctc aacaaagagg gtgagcagga acatgcgtac ggaacctcgt ggggcttctc
     1381 cagccggatg gtcggcggca cggtcatgtc ccacggtgac gatgacgggt tgcgactgcc
     1441 tccagccgtt gcgccctatc aggtcgtcgt ggtccccatc taccggtcgg acgacgagcg
     1501 tgcctccgtg cttgaagtcg tggcaaagat ccgcgacgag ctggccggga acggcgtgcg
     1561 cgtcagggtc gacgatcggg atcagcaccg gccgggattc aagttcgccc agtgggaatt
     1621 gaagggcgtt ccgatccgaa tcgagatcgg ccccaaggac gtggacgcgg gccaggtcgt
     1681 agtggtcgat cgcgttacgc gcgagaagaa ggccttgccg acgtccgaag cgctgtcgtc
     1741 gatggtcggt gctctggaaa gcgttcagaa gtctctgttc gaggacgcac tcaagttccg
     1801 cgaggcgaac acccatgaga tcgagagttt cgacaagttc aaggaaggcg tcgaggccga
     1861 aggtggcttc tgggtcggcg catggtgcgg cgccgcggac tgcgaggcgc gcatagccgg
     1921 agagacgaag acgtcgatcc gggtactccc tctcgagctg gaggatcccg gcgcaccttg
     1981 catgcactgc ggtaagccag gagtcgagcg cgccacctgg gctagggctt attagttcta
     2041 cgcgacggcc gtgcccggca gggaaacagg gccccagagg cgaataaagc agagcaaggc
     2101 cccgatgccg tggcctctac tcgcctatcc aacggagggc ctttctatga cctcgctcgt
     2161 ccgcaagctg tcggtcgcac tgatcggcgg gctcctgctc gccggtctgc tgcccgcctc
     2221 ccccgccgcc gccgacccca ccgggttcgt gatcatgtcc gatggcgtcg agatcgccgt
     2281 caacgtccgg atgccggaca acttcgaaga aggcaagagc tacccgacgg tcttcgagat
     2341 gaacggctac gacggcggtt cgtccgacgg tgacgagccc gagcccgcaa atgagggcag
     2401 ccgggttctc acgaagcagt tctacgacga ctacgtaacc gtgcacgcat ccgtgcgggg
     2461 caccggctgc tccggcggtg agttcgacct gttctcgtgg cgcagcgcgc tcgacggacg
     2521 cgaggtgatc gagtggatcg ccgaccaaga gtggtcgaac ggcgacatag gtatctacgg
     2581 acactcctac ggcgggatca ctggcttcat ggtcgcgtcg acgcggcccc ccgagctgga
     2641 cgcggtttcg gtttcgggcc ttatcgacga cctctaccgc ggcatcgtgt atccgggagg
     2701 cgtcagcaac tacggcttcc cgctcctgtg gaccggcggt gtgcgcaacg tctacgacgt
     2761 cggtggaggt ctcttctacg gcatgtggga agaggacggg agcgttcagc ccggctgcgt
     2821 cacgactgct ccgaccaaga gccggaccgt cctgaacgac gcgatcctgc aggggctgca
     2881 ggagaccgac tcgacctgga tgcaggcccg gtcgatgatc aactacgccg agcggatcaa
     2941 cgtgcccatc cacatctcgg gtgcgtacca ggacgagcag accgggccgc gcggcccgta
     3001 ccacctgttc gaagaggtcg ttaacgctcc cgttcgccgt ctactgatga cgaacggcga
     3061 ccacggcacc cagcaggacc cgttcgtgat ggccgaccgc gtgaagtgga tggaccactg
     3121 ggtccgtaac gtgtccggcg gctacggccc gctgaccgag gatcgctcgt ccgtgaccac
     3181 gttcctcgac atgcgga
//
LOCUS       DATMED010000093         9126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5064058, whole genome shotgun sequence.
ACCESSION   DATMED010000093 DATMED010000000
VERSION     DATMED010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5064058"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..864
                     /locus_tag="VNP73_02315"
     CDS             85..864
                     /locus_tag="VNP73_02315"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HWL64783.1"
                     /translation="MQTGRAPVDQATLEEIATAVRSAARVVVLTGAGISTESGIPDFR
                     GPNGVWTKNPKAERMATLQYYMSDPDVRVEAWKARLEHPGWKATPNPGHLALAELERK
                     GRLHTLVTQNVDGLHQTAGTSEEILVEIHGTMKEVMCMSCGERAAMQKALDRVRAGEQ
                     DPPCRTCGGILKSATISFGQNLVAADLQRAQQAAHECDLMVAIGTSLGVYPVASMPQI
                     ALDQGARLVIVNAEPTPFDPHASWVLRNTISEVLPPLVALV"
     gene            complement(919..1001)
                     /locus_tag="VNP73_02320"
     tRNA            complement(919..1001)
                     /locus_tag="VNP73_02320"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(965..967),aa:Tyr,seq:gta)
     gene            complement(1038..2375)
                     /locus_tag="VNP73_02325"
     CDS             complement(1038..2375)
                     /locus_tag="VNP73_02325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWL64784.1"
                     /translation="MRNVGRIALAIVLLMAALFVLPWPARRQVSSPAHAGIIQLPTPD
                     LSDITKATETPKPDDDDDGGGDDGSGGDDGSGGDDGSGGDDGSGGDEGDDGGLLGGDD
                     SKDGRDGDGKKGKDDDGKKGKDGKGKDDIDLRDGKGKKKKGKDEDVVPGSIANRIPGA
                     YDTRKLVSVAAQLRGLGVSAAEVTDRVYPPFIIAGPASWVDTWHAPRYGPAPGQIRQH
                     EGQDVFCDYGDPILAPVPGTVSFSDGGLGGITARVHEADGSYWYLTHLSDLNTEELGQ
                     GDAVQVGDVLGYCGNSGNASTTPPHVHFGWYQSNGAAKNPMRPLIDWLKAAERRVDIV
                     VSKKTKQRIKNKAVFRTARLFGDSFAPDRSEFRVAGESLWASGSVPSIGTYALAEAAL
                     RAALATHGLDYEAVGSSLSPQAEEEPVAAVLDADSALARLLESRQVSINEAGD"
     gene            complement(2575..4038)
                     /locus_tag="VNP73_02330"
     CDS             complement(2575..4038)
                     /locus_tag="VNP73_02330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWL64785.1"
                     /translation="MQLAQALNASVDWAALAPELVLTAAACIVLVVDLFVPEEGKWIA
                     MPLSAAGIIGTLAAVLALVGTDRLTLAGTFEVTGFALLFKGLFCVIGLIVLAISFHYF
                     RGGRYYQGEYYFMLLCSLLGGLVMSSARDLVTIFIAIELISVPGFVMTALRKNDVRSN
                     EAALKFFLFGVLSSAVMLYGMSLVYGITGETLLTEISAKLAGRGAEPIVTLGVFFVVV
                     GFAFKISAVPFHFWAPDTYEGAPSPVAAFLSTASKIGGFVGLLVLMFIAFPSVADAWR
                     PIFAVLATLTMTVGNLIALRQQHIIRLLAYSGIAQSGYILVCFALIDQGAFAANRQAL
                     ESALIYLAIYGVMDLGAFAAAVSFARRGGSYFISDYAGLWQRSPVLATLLAGFLVSLA
                     GVPPMAGTWAKFFVFKATMQAEVYWLAVVMGINTVIAIWYYLAVVKKMFFEAPEVAEP
                     VEVPYLMRAAMGVVAALLFATFIFPRIITELVERSPI"
     gene            complement(4039..5562)
                     /locus_tag="VNP73_02335"
     CDS             complement(4039..5562)
                     /locus_tag="VNP73_02335"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWL64786.1"
                     /translation="MEWVDEWGLSLATFLPAVGAIVLMFVPPAQERAIKFVGTLFAGL
                     ALLAGVLILARFDYAAAGQIQLEVNSSWIPQIDSRFHIGIDGVSLPLLILSLLVTFLC
                     MIYCYFHIPAPGKPKAFLALILILETGMNGSFIALDLILFFIFWEVVLVPMYFMIGIW
                     GGPRREYAAVKFFLYTLFGSIFMLLGFLALWFNTHTFDMLQLRELAGAGIGSEVFQNL
                     VFLGLFLGFAIKVPMWPFHTWLPDAHTEAPTVGSVLLAAVLLKMGTYGFVRIALPTLP
                     DAAVTYAPIIGILAVIAIIYGALCCLAQQDLKRLIAFSSVGHMGFVMLGIATLTPEGI
                     NGAIFGMVAHGLITGMLFFLAGSIHERYHTREISELGGLLEQIPRFGSVFTYCAIASL
                     GLPGLAGFWGEMLALLGSYQPASPLDPVLFRVLMVGGGIGTVLTAGYLLWTIQRVNLG
                     RVPDKFASGEGIHDVQPLEWFAWAPLLVLILAAGVFPKLVLGVTDEAVTAIMGALGG"
     gene            complement(5596..7620)
                     /gene="nuoL"
                     /locus_tag="VNP73_02340"
     CDS             complement(5596..7620)
                     /gene="nuoL"
                     /locus_tag="VNP73_02340"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWL64787.1"
                     /translation="MTNLIWLQEHGEEVVEASKQGFISDFTWLVPFLPILSFFVIMFF
                     GKRMKSRGHNIGIMAVGFGLFISLLGFIELAAGNAAVEKTWTWFEIGEHLHIEFGMNY
                     DFLTGIMFVVVTGVSLLVHVYSTGYMHDDKRYTIFYAFLSLFTGSMLLMVIANDFMML
                     FVGWELVGVCSYFLIGHYWEEKENSSAAIKAFITNRVGDMGFLFGIFACFILAGTLNI
                     TELNHLAEEGEIGGTALMIAALLLFCGVVSKSAQFPLYVWLPDAMAGPTPVSALIHAA
                     TMVVAGVYMTARMFVLFEHAGAALDVVAVVAAITMLMTAFQALVQDDIKRVLAYSTVS
                     QLAYMMAALGVGAYTAGVFHLFTHAFFKALLFLGAGSLIHAAHTNNMSEMGGMKDYMP
                     HTFRTVMIGSLGLAGIIPLAGFWSKDEIIGGALRSASEGHQAAAWVVFAAALITSFLT
                     ALYMTRACIKTFWGPYRGHGTPHESPGSMVSPLWILAGATLVVGFLGLPVIGPFQDWI
                     TVPGHEHHGFETLYNIVLPAGATLIAFAAIFVGVQMFMHERVGFDILKSPFAWMYRLV
                     ENKYYLDDFYLNGIVRPTQYKLSAATYWTNQKILDGAVNGVALGARALAQPTYAVIDQ
                     QVVDFAVNGAAGLTGFSGGLLRYIQSGNVQRYAAVLFAAIAAFVALFALT"
     gene            complement(7628..7933)
                     /gene="nuoK"
                     /locus_tag="VNP73_02345"
     CDS             complement(7628..7933)
                     /gene="nuoK"
                     /locus_tag="VNP73_02345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWL64788.1"
                     /translation="MPVVFFLVLSALLFSIGVYGVLARQHAVLVLLSIELMLNAVNVN
                     LVAFSAYLQNAGLGGQVFALFVIAVAAAEVGIGLAIVILIYRNRASVDLDDMALMKW"
     gene            complement(7933..8439)
                     /locus_tag="VNP73_02350"
     CDS             complement(7933..8439)
                     /locus_tag="VNP73_02350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWL64789.1"
                     /translation="MAAPWQQGLFWILAVVIAVSAIRVVTTRNVIHAALYLVGTLMGA
                     AGLYMLLVAEFVAWVQVLVYVGAVVVLMLFGLMLTRAPIGKGEFDNDQRPLAALCALA
                     VFGVTSWILIDSFGDKKIDFGGNIRSTTGDVGMMIFRDFVLPFEVVSVLLLAALVGAV
                     VIARRDPQ"
     gene            complement(8439..>9126)
                     /locus_tag="VNP73_02355"
     CDS             complement(8439..>9126)
                     /locus_tag="VNP73_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWL64790.1"
                     /translation="IDYALCMYCGICVEVCPYDALFWSREFEYAEYDIRELTHEKERL
                     SDWMATVLPPPELEVGAELPGQSSLAAAEPAAVPPTPTAAAPAPAAPAPAAPAPAAPA
                     PAAPPAEKPKTETAPEPAPPAAVEAKAPETPAPEPEAPEESPLPQSAQVTVPTEVPKP
                     VALERTEDPQTVYEQVLQEQLDKGSSPQVAEARAKVARVKAERGIKRGPTPVEVDKPV
                     PSAPTEGEGS"
BASE COUNT         1928 a         2895 c         2837 g         1466 t
ORIGIN      
        1 gagccgttcg gcgagtcggc ggcgctgttc gtcgacgccg aggggtaccg aggacaggtt
       61 ggtcatgccc tagcctagcc agccatgcag accggccgag cccccgtcga tcaagccacc
      121 ctggaagaga tagccacagc cgttcgatct gcggcgagag tcgtcgtcct gacgggggcc
      181 gggatctcga ccgagtcggg cattccggac ttccgggggc cgaatggggt ctggacgaag
      241 aacccgaagg cggagcgcat ggcgaccctc cagtactaca tgtcggatcc cgacgtgagg
      301 gtggaggcgt ggaaagccag gctcgaacat cccggatgga aggcgacgcc gaaccccgga
      361 cacctagctc tggccgagct cgaacgaaag ggcaggttgc acacgctcgt gactcagaac
      421 gtggacgggc ttcatcagac cgcgggtacg tccgaagaga tcctcgtcga gatccacgga
      481 acgatgaagg aagtcatgtg catgtcgtgc ggcgagcgcg ccgccatgca gaaggctcta
      541 gaccgcgtga gggccggcga gcaggatcct ccgtgccgca cgtgtggagg catcctgaag
      601 tcggcgacga tctccttcgg acagaacctg gttgcagcag atcttcagcg cgcgcaacaa
      661 gcggctcacg agtgcgacct catggttgcg atcggaacct cgctgggtgt gtacccggtt
      721 gcctccatgc cgcagatcgc gctggatcaa ggagctcgac tggtgatcgt taacgcggag
      781 ccgacgcctt tcgaccctca cgcctcgtgg gtgttgagaa acacgatcag tgaagtgctt
      841 cccccgctgg ttgctctcgt ctagcccaac ttgtcgcgtc agagcgcgct gaaaggttag
      901 tcagttagcc aagaacggtg gcgggaagag gattcgaacc tctgtaggca tcagccggcg
      961 actttacagg ccgctccctt tggccactcg ggcatcccgc cggtagcgcc agagagatta
     1021 accgccggaa acctgcgtta gtccccggct tcgttgatcg aaacctgccg actctctagc
     1081 aagcgagcca gcgcggaatc ggcgtcgagg accgcggcaa ccggctcttc ctcggcttgc
     1141 ggcgacaggc tcgagcccac ggcctcgtaa tccaaaccgt gcgttgccag ggccgctcta
     1201 agagccgcct ccgccaaggc gtaagtgccg atcgaaggaa cggagcccga agcccacagg
     1261 gattcgcctg cgacccggaa ctcgcttcgg tcgggtgcga agctgtcgcc gaacaaacga
     1321 gccgtacgga agacggcctt gttcttgatg cgttgcttcg tcttcttact caccacgatg
     1381 tcgaccctgc gctcggcggc cttcaaccaa tcgatcaacg ggcgcatcgg gttcttggcc
     1441 gcaccgttgg actgatacca accgaagtgc acgtgaggag gcgtggtcga ggcgttgccc
     1501 gaattaccgc aataaccgag cacgtctccg acctgcaccg cgtcgccctg accgagctct
     1561 tcggtgttga ggtcggaaag gtgggtcaga taccaatacg aaccgtcggc ctcgtgcacg
     1621 cgtgcagtga tgccgcccag acctccgtcc gagaagctga cggtgccggg gaccggagcg
     1681 aggatcggat cgccgtagtc gcagaagacg tcttgacctt cgtgttgacg gatctgtccg
     1741 ggggccgggc cgtaacgcgg cgcatgccac gtgtccaccc atgaagcggg tccggcgatg
     1801 atgaagggcg ggtaaacgcg atccgtcact tccgctgcag aaacgccgag acctcgcagt
     1861 tgggcggcta cggaaacaag cttgcgcgtg tcgtaagcac cggggatccg gttggcgatg
     1921 cttccgggaa cgacgtcctc gtctttgccc ttcttcttct tgcccttgcc gtcacgaaga
     1981 tcgatgtcgt ccttgccttt gccgtcctta cctttcttgc cgtcgtcgtc tttgcccttc
     2041 ttgccgtcgc cgtcgcggcc gtccttggaa tcgtcgccgc cgagaagtcc cccgtcgtcg
     2101 ccctcatcgc cacccgagcc gtcatccccg ccggagccgt cgtcgcctcc agaaccgtcg
     2161 tcgcctccag aaccgtcgtc cccgcctccg tcgtcgtcgt catcgggctt gggggtctcg
     2221 gtggccttcg tgatgtcgct gagatcgggc gtggggagct ggatgatccc ggcgtgggca
     2281 ggggaactca cctgccggcg ggccggccac ggcagcacga agagagccgc catgagcagc
     2341 acgatcgcca gagcgatccg cccaacgttt ctcattcggt aatcgccacc tcgccgcatc
     2401 gacgcaaagc aagcgcctac ttctaaggac gcaagcctag gaccttcctc tcactaataa
     2461 gtaacggaaa cgccctcccc taagtaacgg cagggtcaga ccgtcgattt agatactatt
     2521 tgtccgtatt cgggccgaac atagtccgaa cgggtggttt ccccgatcca gcagctatat
     2581 gggggaccgc tcgaccagct cggtgatgat ccgcggaaag atgaaggtgg caaacagcag
     2641 cgccgccacg actcccatcg cggcgcgcat caggtacggc acctcgaccg gctcggccac
     2701 ctccggcgcc tcgaagaaca tcttcttcac gaccgcgagg tagtaccaga tcgctatcac
     2761 cgtgttgata cccatcacga ccgcaagcca gtagacctcg gcttgcattg tcgccttgaa
     2821 gacgaagaac ttggcccagg ttcccgccat cggtggcaca ccggctagcg atacgaggaa
     2881 accggccagc aacgtcgcga ggacgggact ccgttgccat agacccgcgt agtccgagat
     2941 gaagtaggag cctcccctcc gtgcaaacga aactgcagcc gcgaaggcgc caaggtccat
     3001 caccccgtag atcgcgaggt agatcaacgc cgactccagc gcctgccggt tggccgcgaa
     3061 ggctccttga tcgatcagag cgaaacacac gaggatgtaa cccgactgag cgatcccgga
     3121 atacgcgagc agtctgatga tgtgctgctg acggagcgcg atcaggttcc cgaccgtcat
     3181 cgtcagcgtc gcgagcacgg cgaagatcgg acgccacgcg tctgcgaccg acgggaacgc
     3241 gatgaacatc agcacgagca gtccgacgaa tccgccgatc ttggaggcgg tcgaaaggaa
     3301 cgccgccacc ggcgaaggag ccccctcgta cgtgtccgga gcccagaagt ggaacggcac
     3361 cgccgagatc ttgaacgcaa agccgaccac gacgaagaac acgccgaggg tcacgatcgg
     3421 ctcggccccc ctaccggcga gcttggcaga gatctcggtc agcaacgtct cgccggtgat
     3481 cccgtagacc aacgacatcc cgtagagcat cacggccgag gagaggaccc cgaagaggaa
     3541 gaacttcagg gcggcttcat tcgagcgcac gtcattcttc cgcagagcag tcataacgaa
     3601 cccgggcacg ctgatcaact cgatggcgat gaagatcgtc accagatcgc gggcgctcga
     3661 catcacgagg ccgccgagga gagagcacaa gagcatgaag tagtactcgc cctggtagta
     3721 gcgaccgcct cggaagtaat ggaacgagat ggccagcacg atcagaccga tcacgcagaa
     3781 gagacctttg aacaacagtg cgaatccggt cacctcgaac gtccccgcca acgtgagccg
     3841 atcggtccct acgagcgcca ggacggcggc aagcgttccg atgatcccgg ccgccgatag
     3901 cggcatcgcg atccacttgc cctcctcggg cacgaagagg tcgacaacaa gcacgatgca
     3961 ggccgccgcc gtcagcacga gctcgggcgc gagagcagcc caatcgaccg acgcgttcag
     4021 tgcctgcgcg agctgcatct aaccgcccag cgctcccatg atggccgtga cggcctcgtc
     4081 ggtgacgccg aggacgagct tggggaagac cccggccgcc aggatgagca cgagcagtgg
     4141 cgcccacgcg aaccactcga gtggttggac gtcgtggatc ccctctcccg atgcgaactt
     4201 gtcgggaacg cggccgaggt tcacccgctg gatcgtccac agcaggtagc cggccgtcag
     4261 cacggtcccg atccctccgc cgaccatgag caccctgaac aggacagggt cgaggggcga
     4321 ggcaggttgg tacgacccga gcagcgccag catctcgccc cagaacccgg ccagtcccgg
     4381 gagacccaaa gatgcgatcg cgcaataggt gaatacggaa ccgaatctgg ggatctgctc
     4441 caacagccct ccgagctccg agatctcgcg cgtgtggtag cgctcgtgga tcgagccggc
     4501 aaggaagaac aacatcccgg tgatcaatcc gtgggccacc atgccgaaga tcgcgccgtt
     4561 gatcccttcg ggcgtcagcg tcgcgatacc gagcatcacg aatcccatgt gccctaccga
     4621 ggagaacgcg atcagtcgct tgaggtcctg ctgagcgaga cagcacaaag cgccgtagat
     4681 gatcgcgatc acagcgagga tcccgatgat cggcgcgtag gtgacggccg catccggcag
     4741 ggtcggcagt gcgatccgca cgaatccgta ggtgcccatc ttcaggagga ccgccgccag
     4801 caacacggag ccgacggtag gagcttcggt atgggcgtcc ggcagccagg tatggaacgg
     4861 ccacatcggt accttgatcg cgaagccgag gaagagcccg aggaacacga ggttctggaa
     4921 cacttccgat ccgatcccgg cccccgccaa ctcgcgcagc tgaagcatgt cgaacgtgtg
     4981 cgtgttgaac cacagtgcca ggaaacccag cagcatgaag atcgagccga acaacgtata
     5041 gaggaagaac ttgaccgccg cgtactcgcg tctcggccca ccccagatcc caatcatgaa
     5101 gtacatcggg accaggacga cctcccagaa gatgaagaac aggatcaggt cgagagcgat
     5161 gaacgagccg ttcatcccgg tctcgaggat gaggatcagc gccaggaaag ccttgggctt
     5221 cccgggggcc gggatgtgga agtagcaata gatcatgcac aggaacgtga ccagcagaga
     5281 caggatcagc agcggtagcg acacgccgtc gatgccgatg tggaaccgcg agtcgatctg
     5341 cgggatccac gacgagttca cctcgagttg gatctggcct gccgcggcgt agtcgaagcg
     5401 ggccaagatc aggaccccgg caagcagcgc cagcccggcg aacaacgtgc ccacgaactt
     5461 gatcgcgcgc tcttgagcag ggggaacgaa cataagaacg atcgctccaa ctgccggaag
     5521 gaaggtcgcg aggctcaaac cccattcgtc aacccattcc atgaatcagt tccgccccct
     5581 tctcatgtcc acgatctatg tcaacgcgaa caacgcgacg aatgctgcga tcgcagcaaa
     5641 gagcaccgcc gcgtagcgtt gaacattgcc cgattggatg tagcgcaata gtccccccga
     5701 gaaccccgtc agccccgcgg ctccattgac cgcgaagtcg acgacctgct gatcgatcac
     5761 tgcgtaggtt ggctgtgcga gcgcccgcgc tccgagagcg acaccgttca cggcgccgtc
     5821 caggatcttc tggttggtcc aatacgtggc ggccgacagc ttgtactgag tcggtcgcac
     5881 gatgccgttc agatagaagt cgtcgaggta gtacttgttc tcgacgaggc gatacatcca
     5941 tgcgaacggg ctcttgagga tgtcgaagcc gacgcgctcg tgcatgaaca tctgcacccc
     6001 gacgaagatc gctgcgaacg cgatcaaggt cgccccggcc ggcagcacga tgttgtaaag
     6061 cgtctcgaac ccgtggtgtt catgacccgg gaccgtgatc cagtcctgga aggggccgat
     6121 cacgggaaga ccgaggaaac cgaccaccaa cgtcgcgccg gcaaggatcc acagcgggga
     6181 caccatcgag cccgggcttt cgtgcggcgt tccgtggcct ctgtagggtc cccagaacgt
     6241 cttgatgcag gcacgggtca tgtagagcgc cgtgaggaac gatgtgatca gagcggcggc
     6301 gaaaacaacc caggcggctg cctgatgccc ctcgctcgcc gaccgcaacg cgccgccgat
     6361 gatctcgtcc ttcgaccaga acccagccag cgggatgatc cccgcaagcc cgagggatcc
     6421 gatcatgacc gtgcggaacg tgtgcggcat gtagtccttc atcccgccca tctcgctcat
     6481 attgttcgtg tgagccgcgt ggatcagcga cccggccccc aggaagagaa gagccttgaa
     6541 gaaggcgtgg gtgaacaggt ggaacacacc tgccgtgtac gcgccgacgc ccagcgccgc
     6601 catcatgtaa gcgagctgcg aaaccgtgct gtaggcgagt acgcgtttga tgtcgtcttg
     6661 aacgagcgcc tggaacgccg tcatcaacat agtgatcgcg gcaacgaccg ccaccacatc
     6721 cagagccgcc cccgcgtgct cgaacaacac gaacatccga gccgtcatgt agaccccggc
     6781 aaccaccatc gtggccgcgt ggatcagagc agaaacgggc gtgggacccg ccatcgcatc
     6841 cggcagccac acgtagagcg ggaactgagc cgacttgctg accacaccgc agaacagaag
     6901 cagggctgcg atcatcaaag cggtcccgcc gatctcgcct tcctccgcaa ggtggttcaa
     6961 ctcggtgatg ttgagcgtgc cggccaggat gaagcaagcg aagatcccga acaggaagcc
     7021 catgtccccg acccggttcg tgatgaacgc cttgatggcc gcggatgagt tctccttctc
     7081 ctcccagtag tgaccgatca ggaagtaaga gcacacaccg acgagctccc agccgacgaa
     7141 cagcatcatg aagtcgttgg cgatgaccat caggagcatc gaacccgtga acaacgacag
     7201 gaaggcgtag aagatcgtgt agcgcttgtc gtcgtgcatg tatcccgtcg aatagacatg
     7261 gacgagcagg gacacgcccg tgacgacgac gaacatgatg ccggtgagga agtcgtagtt
     7321 catcccgaac tcgatgtgca ggtgttcgcc gatctcgaac caggtccacg tcttctcgac
     7381 cgccgcgttc cccgccgcta gctcgatgaa tccgagcagc gagatgaaga ggccgaaacc
     7441 gaccgccatg atgccgatgt tgtggccgcg gctcttcatg cgcttcccga agaacatgat
     7501 cacgaagaag gaaaggatcg ggaggaaagg aacgagccac gtgaagtcgg agatgaagcc
     7561 ttgcttgctg gcttcgacga cctcctcgcc gtgctcctgc agccagatga ggttcgtcat
     7621 cagctagtca ccatttcatc aacgccatgt cgtccaggtc gacgctcgcg cggttccggt
     7681 agatgaggat gacgatcgcc agaccgatgc cgacctctgc cgccgcgacc gcgatcacga
     7741 acagagcgaa gacctggccc ccgagccccg cgttctgcag gtaggccgag aacgcaacga
     7801 gattgacatt cacggcgttg agcatcaact cgatcgagag aaggaccaga acggcatgct
     7861 ggcgggcgag cacgccgtag acgccgatag agaacagcag cgcggacagg accaagaaga
     7921 agacgaccgg cattactgag gatcccttcg ggctatgacc acggcgccga caagggctgc
     7981 gaggagcaac accgacacca cttcgaacgg caggacgaaa tcgcggaaga tcatcatgcc
     8041 gacgtcgccg gtggtcgaac ggatgttgcc gccgaagtcg atcttcttgt cgccgaaaga
     8101 gtcgatgagg atccacgagg tcacgccgaa gaccgccagc gcgcacaggg ccgccagcgg
     8161 ccgctgatcg ttgtcgaatt cgcccttacc gatcggtgcg cgcgtcagca tcagtccgaa
     8221 cagcatcagg accaccactg ctccgacgta gacgagaacc tggacccaag ccacgaactc
     8281 ggcaacgagc agcatgtaca gaccggccgc ccccatcagg gttccgacga gatagagggc
     8341 ggcgtggatc acattgcgag tcgtaaccac tcggatcgcg gacaccgcga tcacgacggc
     8401 caagatccag aagagacctt gctgccacgg ggcggccatc agctaccttc cccctcggtc
     8461 ggcgcgctgg ggaccggttt gtcgacctcg accggggtcg gtccgcgctt gatcccgcgc
     8521 tcggccttca cacgagcgac cttggcgcgt gcttctgcga cctggggcga agatcccttg
     8581 tcgagctgct cctgcaggac ctgctcgtac acggtttggg gatcctcggt ccgctcgaga
     8641 gcgaccggct tgggaacctc ggtgggaacg gtgacctgag ccgactgcgg caacggtgat
     8701 tcctcggggg cctcaggctc cggcgcgggg gtctcgggtg ccttggcctc cacagcagcg
     8761 ggcggagccg gctcgggggc ggtctccgtc ttcggtttct ctgccggggg ggccgcagga
     8821 gccggcgccg caggagcggg ggccgcagga gcgggggccg caggggccgg agccgctgca
     8881 gtaggggtgg gcggaacggc ggcgggctcg gccgccgcca gactagattg gcccggcaac
     8941 tcggccccga cctccagctc gggtggtggc aagactgttg ccatccagtc cgatagacgc
     9001 tccttctcgt gcgtcagctc acggatgtcg tattcggcgt actcgaactc gcgactccag
     9061 aacaacgcgt cgtaagggca cacctcgacg cagatgccgc agtacatgca cagcgcgtag
     9121 tcgatg
//
LOCUS       DATMED010000094         3298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_840246, whole genome shotgun sequence.
ACCESSION   DATMED010000094 DATMED010000000
VERSION     DATMED010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840246"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VNP73_02360"
     CDS             <1..342
                     /locus_tag="VNP73_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64791.1"
                     /translation="KLAGLGNGDVKITVSATGRAEKITCTNKGGNQAPGQNKPRVATV
                     GQTTIPSNQVKNGSVTFSVTTVGPGLLDGKTAGCPNSNWTGRIDDVSFSSATVKVEQG
                     GSVVLNKTFSP"
     gene            complement(353..1756)
                     /locus_tag="VNP73_02365"
     CDS             complement(353..1756)
                     /locus_tag="VNP73_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64792.1"
                     /translation="MGWVEAVANLKLSLVFALCACLIAGGAPTSAAPSGGTFGPAIDA
                     PHYEGQERCSPKAKPGVLAFQSMVLVTYPGTGAGSISRDCSVGGTSEHKEGRAWDWGV
                     AASDKSDVASVDDLLEWLLKDDRYGNDAALARRLGIMYLIWNKRMWSTWDGWETYCEM
                     KKGECRDPESKSVLHPHTDHVHFSFTWAGAKKETTFWNPNRSYLASFASASDGSGHWA
                     AGANGSILVAGDAGFYGSKNDVFLKQPIVDIAATPSGSGYWLTNSKGKVFPFGDASKK
                     RPDTKMQVAGIASTDTGKGYWLASTQGRVAAVGDAEKFDKLAEEGVKVTDIASTLTGE
                     GYWLFADDGRVFPYGDAGFFGGAAEADLEHPIVSGANYLNEGYWMVTDHGRVFAFGDA
                     RMHGDLTDKELTLPVIGMAPTPTGEGYWLLTAGGRVFDFGDARVFPMASPKIQDSDIY
                     TSELKEEGGQVPSLLLD"
     gene            1854..2084
                     /locus_tag="VNP73_02370"
     CDS             1854..2084
                     /locus_tag="VNP73_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64793.1"
                     /translation="MGEQTKEKQAKESNTCPECNEPIDDLRATCANCGYEYKDEDYGD
                     PEKGNEFLSGSNMTDDGEEITDEGPGAEEGSE"
     gene            complement(2085..2462)
                     /locus_tag="VNP73_02375"
     CDS             complement(2085..2462)
                     /locus_tag="VNP73_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HWL64794.1"
                     /translation="MRFISRYCGVDPQSDGFVDLTPQIQEVVDEAGIHAGRVTVSSTR
                     PDCFLLINENESGLRKDFARLWQRSFAEVQSGPGIAHSSVVLPIIKGTLWTGAWQRVL
                     AFVTDKHTSTEGVVIQAWGIDPE"
     gene            complement(2462..>3298)
                     /locus_tag="VNP73_02380"
     CDS             complement(2462..>3298)
                     /locus_tag="VNP73_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWL64795.1"
                     /translation="LIQAGGKRLRPALVMLAADFGEPQGSTDLAAAAVELVHLATLYH
                     DDVIDQTEVRRGVPTVSARWGLEVAVLVGDYLFARACTLGADAGGEVPKILAEAIARV
                     CEGQIAETATVGRVDRSIDEYLATIKKKTAALFRGACELGASTSGASPSNRAALVEYG
                     ETLGVAFQVVDDLLDLLGSPEVIGKEPGTDLKEGVFTLPVLMGCARDPRFAELLEHER
                     SIDRILPELQANGAIEAALSFTHETADRARAAIDKLDDSRAKETLATVISGVVAQVPV
                     AV"
BASE COUNT          637 a         1063 c         1022 g          576 t
ORIGIN      
        1 aagctcgccg gactcggcaa tggggacgta aagatcacgg tcagcgccac gggccgggcc
       61 gaaaagatca cgtgtacgaa caagggcggc aaccaggccc ccgggcagaa caagcctcgg
      121 gtcgccacgg tcggtcagac cacgatcccg tccaaccagg tcaagaacgg cagcgtgacc
      181 ttctcggtca cgacggtagg acccgggctg ctcgatggga agacggccgg ttgccccaac
      241 agcaactgga ccggccggat cgacgacgtc agcttctcga gcgcaacggt gaaggtcgag
      301 cagggtggct cggtcgtgct gaacaagacg ttcagtccct aagacgtctc gctcagtcga
      361 ggaggaggga gggcacttgt cctccctcct cttttagttc gctcgtgtag atatccgagt
      421 cctgaatctt cggcgacgcc atcgggaaga cccgcgcgtc accgaagtcg aacacgcggc
      481 cccctgccgt gagcagccag tagccctcac cagtcggggt cggcgccata ccgatcaccg
      541 ggagtgtcag ttctttgtcg gtcaggtcgc cgtgcatccg ggcatctccg aatgcgaaga
      601 ctcggccgtg atcggtgacc atccagtagc cctcgttcag gtagttcgcg ccggacacga
      661 tgggatgctc gagatccgcc tctgccgcgc ctccgaagaa cccggcgtcc ccatacggaa
      721 acacccggcc gtcgtcggcg aacagccagt aaccctcacc ggtgagggtt gaggcgatat
      781 cggtgacctt cacgccctcc tcggccagtt tgtcgaactt ctcggcatcg ccaacggctg
      841 caacgcgccc ctgcgtggac gcaagccagt agcccttacc ggtgtcggtc gaagcgatcc
      901 cggccacctg catcttcgtg tcgggacgct tcttggacgc atcgccgaag gggaacacct
      961 ttcccttgct gtttgtcagc cagtagccgg agccactcgg cgttgccgcg atgtcgacga
     1021 tgggttgctt gaggaagaca tcgttcttcg atccgtagaa gccggcatcg cccgccacca
     1081 ggatgcttcc gttcgctcct gcggcccagt gaccactgcc gtcgctagcg gatgcgaacg
     1141 atgcgaggta cgagcggttc gggttccaga acgtggtttc tttcttagcc ccggcccacg
     1201 tgaagctgaa gtgcacgtgg tcggtgtgcg ggtgcaggac cgacttgctc tctgggtcgc
     1261 gacactcgcc cttcttcatc tcgcaatagg tctcccaccc atcccaggtg ctccacatcc
     1321 tcttgttcca gatcaggtac atgatcccga gccggcgcgc taaagcggcg tcgttgccgt
     1381 aacggtcgtc tttcagcaac cactcgagca gatcgtcgac gctggcgaca tcggacttgt
     1441 cggaagcggc gacaccccag tcccaggctc gcccttcctt gtgctcgctc gtgccaccga
     1501 cgctgcagtc gcgcgagatc gagccggcgc cggtgccggg gtaggtcacg agaaccatcg
     1561 actggaaggc gagaacgccg ggcttggctt tgggggagca gcgctcctga ccctcgtaat
     1621 gaggggcgtc gatcgccggc ccaaaggtgc cgccggaggg agccgccgaa gtgggcgcgc
     1681 cgcccgcgat aaggcacgcg cacaacgcaa aaacaaggct cagcttcaga ttcgccacag
     1741 cttcgaccca ccccacggat taaggattcg gtgtcctggg aaatcatcgg cacaggaaac
     1801 acttgacagt aggccccgat cgagtagggc gtctacccgt taggagggtt ctcatggggg
     1861 agcagaccaa ggagaagcaa gcaaaagaga gcaatacctg ccccgaatgc aacgagccga
     1921 tagacgacct gcgagcgacc tgcgccaact gcggctacga gtacaaggac gaggactatg
     1981 gcgatcccga gaagggcaac gagttcctct ctgggagcaa catgacggac gacggcgagg
     2041 agatcaccga cgagggcccg ggggccgaag aggggtcgga gtaattactc ggggtcgata
     2101 ccccacgctt ggatcacgac gccctcagtg gacgtgtgct tatccgttac gaacgccaga
     2161 acccgttgcc acgcgccggt ccacagggtt cctttgatta tcgggagaac gacggaactg
     2221 tgtgcgatac cggggccact ctgaacctcg gcgaacgagc gctgccacaa tctcgcgaaa
     2281 tccttcctca gacccgactc gttctcgttg atcagaagga agcaatccgg ccgcgtggat
     2341 gaaacggtca cccggccggc gtggatgccg gcttcatcga cgacctcttg gatctgaggt
     2401 gtcagatcca cgaagccatc gctctggggg tcgaccccgc aatatctcga gatgaatcgc
     2461 atcagactgc gaccggaacc tgtgccacta cacccgagat taccgtcgcc agggtctcct
     2521 tcgccctcga gtcgtcgagc ttgtcgatcg ctgcacgcgc tcgatcggcg gtctcgtggg
     2581 tgaagctgag ggccgcctca atcgcaccgt tcgcttgcag ttccggaagg atccggtcga
     2641 tcgagcgctc gtgctcgagc agctccgcga accgggggtc acgggcgcaa cccatcagga
     2701 ccggaagcgt gaacaccccc tctttcaggt cggttcccgg ctccttcccg atgacctcgg
     2761 ggctgccgag gaggtccagg aggtcgtcga cgacttggaa cgcgaccccg agcgtctcgc
     2821 cgtattcgac gagagctgcg cggttagagg gcgacgcacc cgatgtggat gcacccaact
     2881 cgcaggctcc tctgaacaga gccgcggtct tcttcttgat cgttgccagg tactcgtcga
     2941 tcgagcgatc cacgcgaccg acggtggccg tctccgcgat ctggccctcg cacaccctgg
     3001 cgatcgcctc ggccaggatc ttcggtacct cgccaccggc atccgccccg agagtgcacg
     3061 cgcgagcgaa tagatagtcg ccgacgagaa ccgcgacctc gagtccccag cgtgcgctca
     3121 cggtcggtac gccgcggcga acctcggttt ggtcgatcac atcgtcgtga tacagcgtgg
     3181 cgagatgaac gagttcaacg gcagcagcgg caaggtcggt cgacccttgt ggctctccga
     3241 agtctgcggc cagcatcacg agagcgggcc gcaagcgctt cccgccggct tggatcag
//
LOCUS       DATMED010000095         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3909975, whole genome shotgun sequence.
ACCESSION   DATMED010000095 DATMED010000000
VERSION     DATMED010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3909975"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VNP73_02385"
     CDS             complement(<1..918)
                     /locus_tag="VNP73_02385"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HWL64796.1"
                     /translation="MVAVLLLWAAAPARAHAIVESTEPAIDEVVPSAPSEIVMHFNEP
                     VEVAFGAIRAYDTSGDRVDTADVDHVAGDATAVRVGLEPDLPDGTYTVTWRVISADSH
                     VIQEAFVFHVGAPGQRPQGIAQEILRGQGTGDALVGALLGIARGIGYASLLLLIGAVS
                     FVTLVWRDTETEDGNGGFLARRRTLISLALAAGIVATIAALVLQGVHAAGLGLSAALD
                     GDVLRGVVDTRFGRVMGLRALFLLVAAVVWFTSSTKERSSRAIDASVLVLGVGALVMT
                     AIAGHAGSTDPVPLNVATDVAHLAAAGIWI"
     gene            complement(963..1730)
                     /locus_tag="VNP73_02390"
     CDS             complement(963..1730)
                     /locus_tag="VNP73_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWL64797.1"
                     /translation="MMDSTRRWLTAIFAGMALAVLVALPAWGHAAYKSSDPADGASFP
                     SPPSQVTAEYTEPPDSASYLRVFDPCGRQVDMGNSSQDAVRNSVTVGMNGDAAGTYRV
                     DWFVNSTKDNHPTRGSFTFTVQNGAPCPGQDEPPDESAPEERDRQPGEPVDRDGTTGG
                     TSGRPGSGGSSGSGGDRGVTTDRGEPGRRPGGDGSRQRDDVDEPFEPGPVAVDGPREP
                     GLLTGIPLGGLLMAYAIAAIIGAAGGLIYAGIMGYRR"
     gene            complement(1750..>2281)
                     /locus_tag="VNP73_02395"
     CDS             complement(1750..>2281)
                     /locus_tag="VNP73_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64798.1"
                     /translation="SGRYETHWYATASDGAAQAGMIDFNVTGGATCTKAVRPDAKEDV
                     DLGFDVVDLRSADTKRGAKVTVTTAAAVKCVDLTDKQTLALKFDTGADDTTDVEGLFV
                     CKAGKIVLELSSKDGSFGKLAVTQPSPAKLKVVLTRHVLLGQVGVIAESTLDSSDCEG
                     KVCADRAPDLGWLNVY"
BASE COUNT          407 a          794 c          695 g          385 t
ORIGIN      
        1 gatccagatg cccgcggccg ccaggtgagc gacgtccgtt gcaacgttca ggggaacggg
       61 gtcggtagac cctgcatgcc cggcgatcgc cgtcatcacc agcgccccga cccccagcac
      121 cagcacgctc gcgtcgatcg cccgcgagga tctctcctta gtgctcgatg tgaaccagac
      181 gacggcggcg acgagtagga agagagctct aaggcccatc acccggccga accgcgtgtc
      241 cactacccct ctcagtacgt cgccgtcgag cgcggccgac agacccagac cggcagcgtg
      301 aaccccctga agcaccagcg ctgcgatcgt tgccacgatg ccggctgcaa gagctagaga
      361 gatgagggtt cgccggcgcg ctaagaatcc cccgtttcca tcttccgtct ctgtgtcccg
      421 ccacaccagc gtgacgaagg aaacggcacc gatgagcagc aacaagcttg cgtagccgat
      481 cccgcgcgcg atccccagca acgcgccgac gagtgcgtca ccagtacctt ggccgcgcag
      541 gatctcctgc gcgatcccct gcggtcgctg acccggcgca cccacgtgaa agacgaacgc
      601 ctcctggatg acgtgtgagt cggcagatat cacgcgccag gtcaccgtgt acgtgccatc
      661 gggaagatcc ggttcgagtc caacccgcac tgcggtcgcg tccccggcga cgtgatcgac
      721 gtcggcggtg tcgacgcgat ctcccgacgt gtcgtacgcc cggatggcgc cgaacgcgac
      781 ctcaacgggc tcgttgaagt gcatcacgat ctcggaaggg gctgaaggca ccacctcgtc
      841 gatcgccggt tcggtggact cgacgatcgc atgggcgcgc gcgggggccg cggcccacaa
      901 caagagaacc gcaaccatcg ctgcaagagc gatcgccttg tgcctggggt tgatcagttc
      961 gctcagcgcc tgtatcccat gatgccggcg tagatcagcc cgccggccgc cccgatgatc
     1021 gcggcgatcg cgtacgccat gagcaggccg ccgagaggga taccagttag gagcccgggc
     1081 tccctcggtc cgtcaacggc caccggtccc ggctcgaaag gttcatcgac gtcatctctt
     1141 tgtcgtgagc cgtctccacc cggtcgtctc ccgggctcgc cgcgatcggt cgtgacacca
     1201 cgatcaccgc ccgaacctga ggatcctccg gacccgggcc gcccggatgt gccaccggtt
     1261 gtgccgtcgc ggtcgacggg ctcgccgggc tgtcgatcgc gctcctccgg agccgactcg
     1321 tccggcggct catcctgccc gggacacggc gccccgttct gaacggtgaa cgtgaacgat
     1381 ccgcgcgtgg ggtggttgtc cttagtggag ttgacgaacc agtcgacacg gtaggtcccg
     1441 gccgcgtccc cattcatccc cacggttacc gagttgcgaa ccgcgtcctg tgaggagttg
     1501 cccatatcca cttgccgacc gcagggatcg aaaaccctga ggtacgacgc gctgtccggt
     1561 ggctcggtgt actccgccgt gacctggctg ggagggctcg ggaagctcgc cccatcggcc
     1621 ggatcggacg acttgtacgc agcatgtccc cacgccggca acgcaacgag gacggccaga
     1681 gccatcccgg caaagatcgc cgttagccag cgtctcgttg agtccatcat cacgacctcc
     1741 agactaggac tagtagacgt ttagccaacc gaggtccggt gccctgtcgg cgcagacctt
     1801 gccctcgcaa tcggacgaat cgagcgtcga ttcggcgatc acgccaacct gacctagcag
     1861 aacgtgcctg gtcaaaacga ccttcaactt cgcggggctc ggctgagtca cagcaagctt
     1921 gccgaacgaa ccgtccttcg agctgagctc gagaacgatc ttgcccgcct tgcacacgaa
     1981 cagcccctcg acgtcggtcg tgtcgtcggc gcccgtgtcg aacttcaaag caagcgtttg
     2041 cttatcggtg agatccacgc acttcactgc ggcggcggtc gttaccgtca ccttggcccc
     2101 ccgcttggtg tccgcagatc gcaggtccac gacgtcgaaa ccgagatcga cgtcttcctt
     2161 cgcgtcggga cgcaccgcct tggtgcacgt ggctccgcct gtgacgttga agtcgatcat
     2221 tccggcctgc gcggcaccgt cggacgcagt tgcataccag tgggtctcgt aacgacccga
     2281 c
//
LOCUS       DATMED010000096         7290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1999833, whole genome shotgun sequence.
ACCESSION   DATMED010000096 DATMED010000000
VERSION     DATMED010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999833"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VNP73_02400"
     CDS             <1..1143
                     /locus_tag="VNP73_02400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWL64799.1"
                     /translation="HETSDSSLFVVPPRQDGGMYVDRAIEFLEKANNELEPELMRAPD
                     ARRLMKAYAKAERLASFGLAALARKVADPDQVAKVTGTSVGKARETVATGKVLADSGE
                     LSMALQQGQVSLDQASEIAKAEASVPGVAGGLVQVAKKESFNVLKEKARAAKLDAEQH
                     NGLAERQHNARSARTYSDALGMININLLLEPHVGTPIVARAQAEAQRLARQVSREDRE
                     SFERYLADAYARLLSGAGKGRAKRPELVVLVSHEVATRGWKDVRRGEMCKIPGVGPVS
                     PQVAKRIAQDAFLSGVFYDGKDLRQVKSWTRSIPREVAIALELGPPPNFDGVSCIDCG
                     NRFRTEFDHVQPKGRGGPTSNPNLRPRCWSCHQAKTKRERAGPVVI"
     gene            complement(1136..2278)
                     /locus_tag="VNP73_02405"
     CDS             complement(1136..2278)
                     /locus_tag="VNP73_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64800.1"
                     /translation="MPADLRLLDDEQRALVQMVREFVDEVVAPAAADYEERSEDPEAL
                     YKHLAELELTGIPYPEEFGGGGQPYSTYLLVVEEIARGYLGFAIGLSVHTLCSFAVAT
                     FGSDGLKREVLTRLCSGEWFGAYSLSEPGSGSDAAALSTKAAKVDGGYKLNGSKVFCT
                     RGNEADAILLMARTGDAGAGGISAFIVSKGTEGFGGSKKEDKMGWRSSPTWMLECSDA
                     FVPEDRLVGKEGEGFRIALASLDSGRLGIAACSIGLAQAALDEALRFTSEREQFGKPV
                     NQNEGVQFMLADMATQVNAGRALYRHAAKLRDAGTDYSLEAAQAKLFCSDAAMRVTTD
                     AVQLHGGYGYITEYPVERFMREAKALQIVEGTNQIQRLVIGRHLTR"
     gene            2308..2616
                     /locus_tag="VNP73_02410"
     CDS             2308..2616
                     /locus_tag="VNP73_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64801.1"
                     /translation="MKIEDRRGEPVGEATVTVDDEELIDLLQGLADLVEGKREHVHVT
                     ESVVGGSHIVFRRGGDEEEAAPIERQMDWWVGPVVLFGALFLIIGLVTFVRWAVNLVT
                     "
     gene            2613..4292
                     /locus_tag="VNP73_02415"
     CDS             2613..4292
                     /locus_tag="VNP73_02415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWL64802.1"
                     /translation="MTALGSRSSALERLAATKSGDPLDLLVVGGGITGAGIALDAASR
                     GLTVGLVERLDFASGTSSKSSKLIHGGLRYLEQREFGLMREACTERDLLRRLAPHLVE
                     PVPFVLPASHRSTRAKYGVGLWAYDALASFRNLKVHKHLDGPEVERMVPPLPRGKIRG
                     GYVFYDCRTDDVRLVMEVLVQAVRYGAIAVNHAVVRDLDGTSEGCSAVVEDSTSGDVF
                     DVRARRVVVAAGVWADRLESLAKQDAPPRLRPSKGIHLVFSRDRIPLSNSAAFIPDSE
                     RKRMLFVIPWLDHVLVGTTDTAYEGSLDHPTVGPEDRAYCLDSINKIFGLGLGEQDIS
                     GAYAGLRPLIAGKRDVTADLSRRHAVYEIAPGILGITGGKLTTYRRMARDAVDRVAED
                     LGSQTRCRTRWIRLGSSDPDRLKVAVARRARRMHIGPQTVDDIVRRYGDRAQDVLTVA
                     EELGLGGPLVGGQVPLAAEAAYCARHEMVVHLSDLLARRTRLALVDPMAGIGEGSRAT
                     EVLGAELLWDHDQRIHEGEAHRLWVENERGLELRSLPTRITGGAQRDAGAV"
     gene            4304..5176
                     /locus_tag="VNP73_02420"
     CDS             4304..5176
                     /locus_tag="VNP73_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL64803.1"
                     /translation="MNQAPYEPVKSFDGSTIAARRLSDESGMPLLVVNAIGANLAPWR
                     KSLIDIVRERPVYTWDHRGLLDSAPPATNRFDPGAQAEDAAAVLDHFDAAKCVLVSWS
                     NGARIALELAYRYPERIEAMVLVCGTYGHAASRLVRNLELFPLIPIAAGIAKHFAGYL
                     EGPLRRFVDRREIAGIIRQSGVVGANADTRALIELLRGVAGSDLKRLLATFEAVVGNT
                     GQELLHGIEAPTLLIAGEYDQFSPMTMQEEMRAAISSCRLDVYERATHYLPLEYPAKL
                     GDDIRKFFKDAGAA"
     gene            complement(5205..6125)
                     /locus_tag="VNP73_02425"
     CDS             complement(5205..6125)
                     /locus_tag="VNP73_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWL64804.1"
                     /translation="MSTTLGPMKLICNSRSGGVTKALPVVEAALKERELEYSIERTER
                     AGHAIEIAHRALSDGTRFLVAVGGDGTIHEVVNGIMKADPTVASEAVLGVVAAGTGSD
                     FVKTFGLPTGPRAAVSHLDGDTSFRIDIGKVTYSSEDGGSQIVRYFPNVAEAGLGAEV
                     VARAVRLPRWLGPTVYLAAFWMTIRKFKITQVRVDLVDRTWEGYMNNLVVANGQFFGG
                     GMKIAPKAAPTDGLLDVQIHHARKREAITIMPKIFKGEHVPHPHIEEAKRVKVSIEAD
                     RPLRIEADGEVLGYTPATFEVVRDAISLKV"
     gene            complement(6122..>7290)
                     /locus_tag="VNP73_02430"
     CDS             complement(6122..>7290)
                     /locus_tag="VNP73_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWL64805.1"
                     /translation="YGGGSGVCEGIVARDYLVIELRALEEIVAFDETSRLVTVQAGVL
                     GPDLSKALASWGFMLGHEPQSVAISTVGGWVATRASGQLSARYGGIERIVAGLEAVLP
                     DGRVVRTKPTPRRSAGPDIASLMLGSEGTLGIVTEVTLRVTPLPAERVDRCVRFQHMA
                     DAVGACRRLAQSDLRPTLVRLYDREDATLFLRSFPDETPDPILLMSFDGRDAKGRADE
                     AVDLSGGSLGDDALVGHWWEHRNDAVDEFRHVMSGEGILGPHALVDTMEVTGTWSALR
                     ELYHAMKEALGREADLVGCHLSHVYPDGSCLYFTLASACDSDEQALSRLERWWEVGME
                     TCIANGGSISHHHGIGRRKARWLPDELDGWWQVLRSVKAAVDPNGIMNPGALGL"
BASE COUNT         1333 a         2352 c         2328 g         1277 t
ORIGIN      
        1 catgaaacgt ccgattcgag cctttttgtc gtaccccctc gtcaggatgg gggcatgtat
       61 gtggataggg caatcgagtt cctcgagaag gccaataacg agctagagcc cgagttgatg
      121 cgggcgcccg acgcacgccg attgatgaag gcgtatgcga aggccgagcg gctggcgtcg
      181 ttcggtttgg cggccctggc tcggaaggtt gccgatccag accaggttgc gaaggtcacc
      241 gggacgtcgg tgggcaaggc cagagaaacg gtggcaaccg gaaaggtcct ggccgattcc
      301 ggagagctga gcatggcgct gcaacaaggg caggtctctc tggatcaggc aagcgagatc
      361 gccaaggccg aagcctccgt gcccggcgtg gccggtggat tggtccaggt ggccaagaaa
      421 gagtcgttca acgttctcaa agagaaggcg cgggccgcca agctcgatgc cgagcagcac
      481 aacggcctcg cggaacgcca gcacaatgcc cgcagcgccc gcacctattc ggatgcgctg
      541 gggatgatca atatcaatct cctgctggag cctcatgttg ggactcccat cgtggcccgg
      601 gcgcaagccg aggcccaacg gctcgcccgc caggtgagca gggaagaccg agagtcgttc
      661 gagcgctacc tcgccgatgc ctacgcaagg cttctgtccg gcgcgggcaa gggacgtgcc
      721 aagcgaccgg agttagtggt gctggtgagt cacgaagtcg ccacgcgcgg ttggaaagac
      781 gtgcgcaggg gcgagatgtg caagatcccc ggtgtgggac cggtgtcacc tcaggtggca
      841 aagcgcatcg ctcaggacgc ttttctctcg ggcgtcttct atgacggcaa agacctccgg
      901 caggtcaaga gttggacgcg ttcgatcccg cgcgaggttg cgatcgcttt ggaactgggc
      961 cctccgccca acttcgacgg tgtttcatgc atcgattgcg gcaatcgctt ccggaccgaa
     1021 ttcgatcacg tgcagcccaa gggcagaggt gggccgacct cgaatccaaa cctcagaccg
     1081 cgctgctgga gctgtcacca ggccaagacc aagcgcgagc gtgcggggcc ggtggttatc
     1141 tagtcaggtg gcggccgatc accagacgct ggatctgatt cgttccttcg actatctgca
     1201 gagccttcgc ttcgcgcatg aaacgctcga ccgggtattc ggtgatgtag ccgtagccgc
     1261 cgtgcaactg aacggcatcg gtggtgacgc gcatggcggc gtcggagcag aacagcttcg
     1321 cttgagcagc ctccagcgag tagtccgtgc ccgcgtcgcg gagcttggcg gcgtggcgat
     1381 agagcgcgcg cccggcattc acctgcgtcg ccatatcggc cagcatgaac tgcacccctt
     1441 cgttctgatt cacgggcttg ccgaactgtt cgcgttcgga cgtgaaacgc agagcctcgt
     1501 cgagggccgc ctgtgcgaga ccgatcgagc atgcggcgat cccgagccgg ccgctgtcga
     1561 gcgatgccag tgcgatacgg aatccttcgc cctccttgcc cacgaggcga tcctcgggga
     1621 cgaaagcatc gctgcactcc agcatccagg tgggagacga gcgccagccc atcttgtctt
     1681 ccttcttgga acctccgaag ccttccgttc ccttggacac tatgaaagcg ctgatcccgc
     1741 ctgcccccgc atccccggtt cgagccatca gcaagatggc gtcggcttcg ttaccccggg
     1801 tgcagaagac cttggatccg ttcagcttgt agccgccgtc gaccttggcg gccttggtcg
     1861 aaagggctgc agcgtcggag ccggagcccg gctcggaaag tgagtaagcg ccgaaccact
     1921 cccccgagca caagcgcgtc agcacttccc gcttgagtcc gtcgctaccg aaagtggcga
     1981 cggcgaaaga acaaagcgtg tgaacggata ggccgatcgc gaaccccaag taaccgcgtg
     2041 cgatctcctc gacgacgagc agataggtcg agtatggctg gcccccacct ccgaactcct
     2101 ccggataggg gatgcccgtc agctcgagtt cggcgaggtg cttgtaaaga gcctccgggt
     2161 cctcgctgcg ttcttcgtaa tccgccgccg ccggtgcgac tacctcgtct acaaactccc
     2221 ttaccatctg aacgagagca cgctgctcat cgtcgagcag cctcagatcg gccggcatgg
     2281 gccggacact acccggagag atcactggtg aagatcgaag acaggcgcgg agaaccggta
     2341 ggcgaggcga cggtaaccgt cgacgacgag gagctgatcg acctgctcca gggtcttgcc
     2401 gatctcgtgg agggaaagag agagcacgtg cacgttacgg aatcggtcgt cggtggatcc
     2461 cacatcgtgt tccggcgtgg gggcgacgag gaagaagctg caccgatcga acgccagatg
     2521 gactggtggg tcggaccggt cgttctgttc ggtgctctgt tcttgatcat cggtctggtg
     2581 actttcgttc gctgggccgt caacctcgtg acatgaccgc actcggttcg cgctccagcg
     2641 ccctggagag actggccgcc accaagtcgg gagaccctct cgacctgctc gtcgtgggtg
     2701 gcgggataac gggcgcgggg atcgctctcg atgctgcatc gcgcgggctc acggtgggcc
     2761 tggtcgagag gctcgacttc gcatccggga catcaagcaa gtcgtccaaa ctgatccatg
     2821 gaggcctgag atacctcgaa caacgcgagt tcggactgat gcgtgaagcc tgcacggaac
     2881 gggacctcct acgacggctg gctccgcatc tggtcgaacc cgttccattt gtgctgcccg
     2941 cttcgcatcg ctcgacccgc gccaagtatg gggtgggtct gtgggcatat gacgccctgg
     3001 cgagcttccg caacctgaag gtgcacaaac atctcgacgg tccggaggtc gagcgcatgg
     3061 tcccgccatt gccgcgcgga aagatccgag gtggctacgt cttctacgac tgccgcaccg
     3121 acgacgtgcg tctggtgatg gaggttctcg tacaggcagt gcgctacggc gctatagcgg
     3181 tcaaccacgc cgtcgtaagg gatctggacg gaacctccga gggctgctcg gccgtggtcg
     3241 aagactcaac gtccggcgat gtgttcgacg tgcgggcacg acgcgtggtg gtagcggcgg
     3301 gagtatgggc cgaccgcttg gagtctctgg cgaagcaaga tgcgcccccc cggctgcggc
     3361 cgtccaaggg gatccacctc gtgttctcgc gggacaggat ccccctgtcc aactccgcag
     3421 ccttcatccc ggactccgaa agaaagcgca tgctgttcgt gatcccgtgg ctcgatcatg
     3481 tcttggtcgg cacgaccgac acggcctacg agggcagcct cgatcacccg accgttgggc
     3541 ccgaagaccg cgcctactgc ctcgactcga tcaacaagat cttcgggtta ggtctcgggg
     3601 agcaagacat atccggtgcc tacgcggggc tgcggccgct gatcgccggc aagagagatg
     3661 tgaccgccga cctgtctcgc cggcacgccg tttacgagat agcgccgggc atccttggga
     3721 tcaccggagg caaactgact acctaccgac gcatggcacg cgacgcggtg gaccgggtgg
     3781 ccgaggacct tggttcccaa actcgttgcc gcacgcgttg gatccgattg ggctccagcg
     3841 accccgatcg tctcaaggtc gcggttgcgc gacgcgcccg gcggatgcac atcggccccc
     3901 agaccgtcga cgacatcgtc cgcaggtacg gcgatcgcgc acaggacgtg cttactgtgg
     3961 ccgaagaatt aggcctcggc ggccctctcg tcggggggca ggtaccgctg gcagccgaag
     4021 cggcctattg cgcacggcac gagatggtgg tgcacctgtc ggatctgctc gcccgcagga
     4081 cccgccttgc actggtggat ccgatggcgg ggatcggcga gggatcccga gcaaccgaag
     4141 tgttgggggc cgaactgcta tgggaccacg accagcggat ccacgaagga gaagctcaca
     4201 gactctgggt cgagaacgaa cgcggcctgg aactcagatc gctaccgacg cgcatcaccg
     4261 gcggcgcgca gcgcgacgcg ggggccgtgt gaccacgggc cgggtgaatc aggcaccgta
     4321 cgaacccgtt aagtccttcg acggatcgac gatcgccgcc cggcggttgt cggacgagag
     4381 cggcatgccg ctgctcgtcg tcaacgcgat cggagcgaac ctcgcaccgt ggcgtaagag
     4441 cctgatcgac atcgtgcgtg agcgtcccgt gtacacgtgg gatcaccgag gactgctcga
     4501 ctccgctcct cccgcgacca ataggttcga ccccggggct caagcggaag atgcggccgc
     4561 ggtgctggac cacttcgatg cggccaagtg cgtgttggtg tcgtggagca acggtgcacg
     4621 gatcgcactc gagctcgcat acagatatcc cgaacgcata gaagcgatgg tgctcgtgtg
     4681 cggcacctac ggccacgccg catcgcgcct ggtgaggaac ctggaactgt tcccacttat
     4741 cccaatcgcg gcggggatcg cgaaacattt cgccggctac ctcgaaggac cgctccgtcg
     4801 attcgtcgac cggcgcgaga tcgcagggat catcaggcaa tccggggtcg tcggtgccaa
     4861 cgccgatacg cgtgccttga tcgagctgtt acgaggcgtg gccggatccg acctgaagag
     4921 gcttctggcg accttcgagg cagtcgtcgg caacaccggt caggagctct tgcacgggat
     4981 cgaagcgccc actctcctga tcgccggtga gtacgaccag ttctccccca tgacgatgca
     5041 ggaagagatg cgcgcggcca tctcgtcgtg ccgcctggac gtctacgagc gcgcgacgca
     5101 ctatctgccg ctggaatatc ccgccaaact gggcgacgac atccgcaagt tcttcaagga
     5161 cgccggcgcg gcctagttgg cagccggtag tccgaaaacc atcgctagac cttcagcgag
     5221 atagcgtcgc gtacgacctc gaacgtggcc ggcgtgtagc ccagcacctc accgtcggct
     5281 tcgatccgca gcggccggtc ggcttcgatg gagaccttca cccgcttcgc ctcttcgatg
     5341 tgcgggtggg gaacgtgctc ccccttgaag atcttcggca tgatcgtgat cgcctcccgc
     5401 ttgcgcgcgt gatggatctg tacgtcgagc agaccgtctg tcggcgctgc cttcggagcg
     5461 atcttcattc cccctccgaa gaactgtccg ttcgcgacca cgaggttgtt catgtacccc
     5521 tcccaggtac ggtccacaag gtcgactctc acttgcgtga ttttgaattt ccggatcgtc
     5581 atccagaagg ccgcgagata gacggtcggt ccgagccagc gcgggagcct caccgcgcgc
     5641 gcaacaacct cggcccccaa cccggcctcg gcaacgttcg ggaagtaccg gacgatctgt
     5701 gatcccccat cctccgagga gtaggtcacc ttcccgatgt cgatccggaa cgacgtatcg
     5761 ccgtccaggt gcgagacggc ggcccgagga ccggtcggca acccgaaggt cttcacgaag
     5821 tccgagccgg tccccgctgc gacaaccccg agcacggcct cgcttgccac cgtcggatcg
     5881 gccttcatga tcccgttgac gacctcgtgg atcgtgccgt ccccgccgac cgccacaagg
     5941 aaccgcgtcc cgtcggacag agcgcggtgc gcgatctcga tcgcgtgacc ggcccgctcg
     6001 gtgcgttcga tcgagtactc gagctcgcgc tctttcagtg cggcttccac tacgggaaga
     6061 gctttcgtaa caccaccgga gcgcgagttg cagatgagct tcatcggtcc gagggtggtg
     6121 ctcataaccc cagagctccg gggttcatga tcccgttcgg gtctacagca gccttgaccg
     6181 acctgagtac ctgccaccat ccgtcgagct cgtcgggcag ccaccgagct ttccggcggc
     6241 cgatcccatg gtgatggctg atcgagccgc cattcgcgat gcaggtttcc ataccgacct
     6301 cccaccagcg ctccagccgg gacagcgcct gttcgtcgct gtcgcacgcc gatgccagtg
     6361 tgaagtagag acacgaacca tcgggataga cgtgcgagag atggcagccg accagatcgg
     6421 cttcccgacc cagtgcctcc ttcatagcgt ggtacagctc gcgcaaagcc gaccaggtcc
     6481 cggtcacttc catcgtgtct acgagcgcgt gggggccgag gatgccttcg cccgacatca
     6541 cgtgccggaa ctcatcgacc gcgtcgttgc ggtgctccca ccagtggccg acgagcgcgt
     6601 cgtcgccgag ggacccgccg ctcagatcta cggcctcgtc ggctcggccc ttcgcgtcgc
     6661 ggccgtcgaa cgacatcaac aggatcggat cgggggtttc gtccgggaaa ctccgcagga
     6721 aaagggttgc gtcctcgcgg tcgtagagcc gcacgagggt cgggcgcaga tccgactgtg
     6781 ccagtcgcct acaggcgccg accgcgtcgg ccatgtgttg gaaccgcacg cagcggtcga
     6841 cccgttcggc cggcagaggc gtgacgcgta gcgtcacctc ggtgacgatg ccgagcgtcc
     6901 cctcggagcc cagcatcaag gaagcgatgt cgggcccggc cgaccggcgc ggcgtcggct
     6961 tggttcgaac gactcgcccg tcgggaagga ccgcctccaa ccccgcgacg atgcgttcga
     7021 tgcccccata gcgcgccgag agctggccgc tcgcccgggt cgccacccat ccaccgaccg
     7081 tcgatatcgc caccgattga ggttcgtggc cgagcatgaa cccccacgat gcgagcgcct
     7141 tgctcaggtc gggaccgaga accccggctt gaaccgtgac caacctgctg gtttcgtcaa
     7201 aggccactat ctcttccagg gcgcgcaatt cgatcaccag gtagtcccgg gcgacaatcc
     7261 cttcacatac tcccgaccca cccccatagg
//
LOCUS       DATMED010000097         5956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_80482, whole genome shotgun sequence.
ACCESSION   DATMED010000097 DATMED010000000
VERSION     DATMED010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80482"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VNP73_02435"
     CDS             complement(<1..1105)
                     /locus_tag="VNP73_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL64806.1"
                     /translation="MRRIIAILGVALSVTGVLTASAQSPDPLLNDSLTAVSDLTKAEH
                     CKLRDASDDDAADGIELGYVHCDDGLPPEGGGSLSIPVPAKYAAVSGDDWTGLPPPAG
                     VEEVNASPDDIQPERDNRISLDVNITLPPSRSAQTLVGATVPVIKPPKKGYPVIVLMH
                     GCCGGNRTNWEAATVDAEGELWHHSNAWFASRGYVVITYTARGFRNADEQGSTGTTQL
                     DSRRYEINDFQYLAGLLADHDAQARAAGLKPLFNINPKKIAAVGGSYGGGFSWLAITD
                     PKWTSPVGQVPMRLGAAVPKYGWTDLVEALVPSGHYFDEDPATGESFIAPTDPKQALS
                     RSPLGVVKQSIVSGLYVTGNASNGDHTTFPQYLD"
     gene            1307..2383
                     /locus_tag="VNP73_02440"
     CDS             1307..2383
                     /locus_tag="VNP73_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWL64807.1"
                     /translation="MKLRFFEDAHILVVDDVDANTYFLERILTDAGYVNIETVNDARQ
                     VLPVFETFQPDIVLLDIRMPHLDGFDVMHQLMARMDHDTPVPIIVLTADLDPDKRLQA
                     LTLGAKDFVTKPFDKVEVRLRIMNQLETRALQLRLNKQRNNLEEEVSARTKELNDARL
                     EMLERLALAAEYRDDATGQHALRVGSFSAVLASALELPDEDVECIRKAAQLHDIGKIG
                     IPDHILQKPGRLTHAEFAFIKRHTDIGARILSGSTTRLLQMAEDIALSHHERWDGTGY
                     AGLPAKHVPICARIVAVADAFDAMTHDRPYRKALPPELAAAEIKNQNARQFDPDVVDA
                     FLRTESLTGLHEVLAREVVDLTGS"
     gene            complement(2388..2660)
                     /locus_tag="VNP73_02445"
     CDS             complement(2388..2660)
                     /locus_tag="VNP73_02445"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWL64808.1"
                     /translation="MDDELVQVFSASHMDAEFVRSILEANEIPAMLSGEGHSSAYPVN
                     VGGLGGGRVLVRKEDEAKAREIIEGVERGDYALAEDEDVEGEPVED"
     gene            2708..3094
                     /locus_tag="VNP73_02450"
     CDS             2708..3094
                     /locus_tag="VNP73_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64809.1"
                     /translation="MPRFPAPRSKTEARNEWATDIRDRLPEWLERVAPENSRGVVAGN
                     GGVELLVGAAKVASFLPTGVGMSVYLYGSSDTEVKRIERLCERAGVPEDVRPSRTSKY
                     VLIKVKDDETLEHVAEALRLHCLGRK"
     gene            3099..4343
                     /locus_tag="VNP73_02455"
     CDS             3099..4343
                     /locus_tag="VNP73_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWL64810.1"
                     /translation="MTRVARIELYHFSSPLPATFRPSWIPGFPQNENRATLLRVLTDD
                     GVEGWTAGPAIGKERSGLGQLIGPYLIGEDATDIELIQQRLREISYLGWRNWWIEPAF
                     WDIKGKLEGKPVWKLLNPDVHPTVRTVYASTGEVREAPARIEEAAARFEEGFRTIKIR
                     VHEDEEADAAQVRGVAEAIGDRMAIGVDANQAWRVTAVADAPLWDLARAKRFADLCAD
                     AGISWIEEPLPMDAYEDLAALTEYSKVPIAGGELHTSAYPELKMMVDRRCYSIFQPDA
                     MFTGGIQQTWRLIQACKAAGLEYTPHTWTNGIGFAVNLQLHAAVDAEHPLEYPIDPPG
                     WVPEARDAMLEQPFLHNKGTLDVPDKPGLGIEISKKALRKWADRYFVMDRKRLAFFAL
                     RDRGLKAAKEMDAAKKERLKKG"
     gene            4416..>5956
                     /locus_tag="VNP73_02460"
     CDS             4416..>5956
                     /locus_tag="VNP73_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64811.1"
                     /translation="MRIRRKLIAGLGIACLFATLAPVGGGAQAAEPPNISAIDATFSF
                     PVTTYGVLATDPDGDSITYRWEKTKERDCGTLTGANTDTFKWSHPHPPCPNEDFHPGT
                     ITVTVSDGTFNCKAVYPNGSREGIGPEPEPCTSVSDPVSVFCPGFQANEGWFEPTQGV
                     WQDDDYWRDRSGKQLTRVNPKSYVAELDMVRNRPTVLMGIDHAVGEAKHRHERDQIVM
                     RGVTTGSKKVPVKMRFSVTDSSGTQEIYTTPNLTRLPLLKPCGKRQRFRINLRTRTGV
                     PRASTFTFNGSGPYTLENELIRGDTNDGTGMKVQVFGNVVETSFPTTSFLGTTLSTTD
                     QAGSDSLLANAVRLANETSTRLPDYLPLPSGGVNTLVYPIVFHAETTLETGRQNWLAW
                     AESNPSAPVTARELRIFRDQALAATAASYFAVFAHLGRAGRMVVLLNDADLERINPWP
                     FNINGLATSEKVVLVQDDESHITVMHELVHTIPHVFLDDGQQPCGPPNYHNRELNTAN
                     GLQLTV"
BASE COUNT         1226 a         1881 c         1799 g         1050 t
ORIGIN      
        1 cgtcgaggta ctgggggaag gtcgtgtgat ccccattcga tgcgttgccc gtcacgtaca
       61 agcccgacac gatgctctgc ttcacgacgc cgagggggga tcgcgacagc gcttgcttgg
      121 ggtcggtcgg agcgatgaag ctctcgcccg tcgccggatc ctcgtcgaag taatgaccgg
      181 acggaacgag ggcctcgacc aggtcggtcc atccgtactt cggcaccgcg gcaccgagtc
      241 tcatcggtac ctgccccact ggactggtcc acttcggatc ggtgatcgcg agccacgaga
      301 acccgccacc ataggagcct cccacggccg cgatcttctt cgggttgatg ttgaacaacg
      361 gcttgagtcc cgcagcacgc gcctgcgcat cgtgatcggc gagaagcccc gctagatatt
      421 ggaagtcgtt tatctcgtag cgcctcgaat cgagttgtgt cgtgcccgtc gagccctgct
      481 cgtcggcgtt gcggaaccct cttgccgtgt aggtgatcac tacgtaaccg cgcgaagcga
      541 accacgcatt cgagtgatgc cagagctcgc cctcagcgtc tacggtcgct gcttcccagt
      601 tcgtcctgtt gccgccacag cacccgtgca tgagcacgat caccgggtac cccttcttgg
      661 gaggcttgat caccggaacg gtcgcgccca cgagcgtttg cgccgacctg ctcggaggca
      721 acgtgatgtt cacgtccagg ctgatgcggt tgtcccgctc gggctggatg tcgtcgggtg
      781 atgcgttcac ctcttcgact ccggccggtg gaggcaaacc ggtccagtcg tcaccggaaa
      841 cggctgcgta cttggccggc actgggatcg acagtgatcc gccgccctcg ggcggtaatc
      901 cgtcgtcgca atgcacgtaa ccgagttcga tcccgtccgc agcgtcgtcg tcggatgcgt
      961 cgcggagttt gcagtgctcg gctttcgtca ggtccgacac cgccgtgagg ctgtcgttca
     1021 gtaaggggtc aggtgactga gcagatgctg tcaggacccc ggtcactgaa agggcgacgc
     1081 caaggatggc gatgatccgt ctcactcgtt tcaagatagc aagaggcgcg cgttacacaa
     1141 ccagttggga ttttttgtcc gaattgaacc ggttagcgcg tattgcgatt tgccttccac
     1201 taactagtga gttcaggcta tgcctaagac cacccgatgt aactacgtaa tacgtaatcg
     1261 acgcagtcga ttacccagtt aggagcatga gaggcaagga gtgagattga agctgcgttt
     1321 cttcgaggat gcccacatcc tcgtcgtgga tgacgtcgat gccaacacct atttcctgga
     1381 acgcatcctc accgatgccg gttacgtgaa catcgagacg gtcaacgacg cgcgccaggt
     1441 ccttccggtc ttcgaaacgt ttcagcccga catcgtcctt ctcgatatcc ggatgcctca
     1501 cctcgacggc ttcgacgtga tgcaccaatt gatggcacgg atggatcacg acacacccgt
     1561 cccgatcatc gttcttaccg ccgacctcga cccggacaag agactgcaag cactcacgtt
     1621 gggagcgaag gacttcgtca ccaaaccgtt cgacaaagtc gaggttcgct tgcggatcat
     1681 gaatcagctc gaaacgcgag ccctgcagct gcgactcaac aaacagagga acaatctgga
     1741 agaagaggtc tccgcacgga cgaaggagct gaacgacgcg cggctcgaga tgctcgagcg
     1801 cctcgctctg gctgcggagt accgcgatga cgcgaccggt cagcacgccc tgcgggtcgg
     1861 ctccttctct gcagtgctcg catctgcctt ggagctaccc gacgaggacg tcgaatgcat
     1921 ccgcaaggcg gcccaattgc acgacatagg caagataggg atacccgacc acatccttca
     1981 gaagcccgga cgactgaccc atgccgagtt cgcgttcatc aagagacaca cggacatcgg
     2041 cgcgcgcatc ctttcgggga gcacgacgag gcttctccag atggccgagg acatcgcctt
     2101 gagccatcac gagagatggg acggaaccgg ctatgccgga ctgcccgcca aacacgtgcc
     2161 gatctgcgca cggatcgttg cagttgcaga tgccttcgat gcgatgacac acgaccgtcc
     2221 gtacaggaag gcgctgcccc cggaactagc agcggcggag atcaagaacc agaacgctcg
     2281 ccagttcgat ccggatgtgg tggacgcatt cctgcggacc gaatccctca ccgggttaca
     2341 cgaagtcctt gcgcgcgagg tcgtcgacct aaccggaagc taaggaacta gtcctctacc
     2401 ggttcgcctt cgacgtcttc gtcttcggcg agcgcgtagt cacctctttc gaccccttcg
     2461 atgatctcgc gcgccttagc ctcgtcttcc ttccgtacga gcacccggcc cccgcccaaa
     2521 cctccgacgt tcaccggata ggcactcgag tgcccctcgc cactgagcat cgccgggatc
     2581 tcgttcgcct cgaggatcga ccggacgaac tcggcgtcca tatgcgaagc ggagaagacc
     2641 tgaacaagct cgtcatccat gaaagtgagc ctagaacggc ttcggacgtc tgacgtagcc
     2701 tggacaaatg ccgcgattcc cggctcccag atcgaagacc gaggcccgca acgaatgggc
     2761 gacggacatc agggacaggc tgcccgagtg gctcgagcgg gttgccccgg agaattcgcg
     2821 cggcgtcgtc gccggcaacg gaggtgtcga gctgctcgtc ggcgccgcca aggtcgcctc
     2881 gttcctgccc actggggtcg gcatgagcgt ctacctctac ggctcgtccg acaccgaggt
     2941 gaagcgcatc gagagactgt gcgagcgggc gggcgttccc gaagacgtgc gaccctcacg
     3001 gacctccaag tacgttctga tcaaggtcaa ggacgacgag accctggagc acgtcgccga
     3061 ggcgctacga ctccactgtc taggacggaa gtgagcgcat gacccgagtt gcccgtatcg
     3121 agctgtacca cttctcctca cctctcccgg cgacgttccg tccgtcgtgg atccccggct
     3181 tcccccagaa cgaaaaccgc gcgactctgt tgcgcgtcct gaccgacgac ggagtcgagg
     3241 gatggacggc aggtccggcg atcggcaagg aacgcagcgg gctggggcag ctgatcgggc
     3301 cctacctgat cggggaagac gccacagaca tcgaactgat ccagcaacgt ctacgcgaga
     3361 tctcgtacct cggctggcgc aactggtgga tcgaaccggc tttctgggac atcaagggca
     3421 agctcgaggg caagccggtg tggaagctgc tcaaccccga cgtccacccg accgttcgaa
     3481 cggtctatgc atcgaccggt gaggtcagag aggcgccggc gcgcatcgaa gaagcggccg
     3541 cccgcttcga agaggggttc cgcacgatca agatcagggt gcacgaggac gaggaagcgg
     3601 acgccgcgca ggtgcgcggg gtcgcagagg cgatcggcga tcgcatggcg atcggcgtcg
     3661 acgcgaacca ggcgtggcga gtcacggccg tggcagatgc tcctttgtgg gacctggccc
     3721 gcgcgaaacg cttcgccgac ctgtgcgccg atgcgggcat ctcatggatc gaagaaccac
     3781 taccgatgga cgcgtacgaa gacctagccg cgctgaccga gtactcgaag gtaccgatcg
     3841 cggggggcga gctacacacc tccgcgtatc cggagctaaa gatgatggtc gaccgccggt
     3901 gctattcgat cttccaaccc gatgcgatgt tcaccggggg catccaacag acgtggaggt
     3961 tgatccaggc ctgcaaggcc gcgggtctcg agtacacgcc gcacacgtgg accaacggca
     4021 tcggtttcgc ggtgaacctg caactgcatg ccgcagtgga tgccgagcac ccgctcgagt
     4081 atccgatcga ccctcccggg tgggtacccg aagcacgcga cgcgatgttg gaacaaccct
     4141 tcctgcacaa caagggaacg ctcgacgtcc cggacaaacc ggggctgggc atcgagatca
     4201 gcaagaaagc actgaggaag tgggccgacc gctacttcgt gatggaccgc aaacggttgg
     4261 cgttcttcgc tctccgggac cgaggcctca aagcggctaa ggagatggac gccgccaaga
     4321 aggagcgtct caagaaggga taggccccca acccccttga ccctcgcggc cgacccgacc
     4381 gatgctgttt cggcggcgag aagggggaca cccagttgcg catccgacgg aagctcatag
     4441 ccggcctcgg catcgcttgc ctgttcgcaa cgctggcgcc ggtcggagga ggcgctcaag
     4501 cagcggagcc gcccaacatc agcgcgatcg acgcaacctt ctccttcccc gttactacct
     4561 atggcgtttt ggcgaccgac cccgacggcg acagcatcac ctatcgctgg gagaagacca
     4621 aggagcggga ttgtggaacg ctcactggag ccaacaccga cacgttcaag tggtcacacc
     4681 cgcacccgcc gtgtcccaat gaggacttcc atcccggcac catcaccgtc acggtttcgg
     4741 acggcacgtt caattgcaaa gcggtctatc cgaacgggtc gcgcgagggg atcggtcccg
     4801 aacctgaacc gtgcacatct gtgtccgacc cggtgtcggt cttctgtccc ggcttccagg
     4861 ctaatgaggg ctggttcgag ccgacccagg gcgtgtggca agacgacgat tactggcgcg
     4921 acagatccgg gaaacagcta acccgagtca accccaagag ctacgtcgcc gagctggaca
     4981 tggtccgcaa ccgacccacg gtactcatgg ggatcgatca cgcggtcggc gaagcgaagc
     5041 acaggcacga gcgcgaccag atcgtgatgc gtggcgtgac gacgggaagc aagaaggtgc
     5101 cggtgaagat gagattctcg gttaccgact cgtcgggaac ccaagagatc tacacgacgc
     5161 caaacctcac ccgtttgcct ctgctgaagc cttgtggcaa acgacagcgt ttcaggatca
     5221 accttcgaac ccgcacgggg gtaccacgag cctcgacctt cactttcaac ggatcgggcc
     5281 cgtacacgct cgagaacgaa ctcatccgag gagataccaa cgatggcacc gggatgaagg
     5341 ttcaggtctt cggaaacgta gtcgagacga gtttcccgac cacgtcattc ctcggcacga
     5401 cgctgagcac gacggaccag gcggggagcg actctttgtt ggcgaatgcc gttcggttgg
     5461 ccaacgaaac gagcactcgg ttgcctgatt acctgcctct accttcgggg ggagtgaaca
     5521 cgctggtcta cccgatcgtg ttccacgccg agacgacact cgagaccggg cgacagaact
     5581 ggctggcttg ggccgagtcg aacccctcgg caccggtcac ggctcgggag ctgcgcatct
     5641 tccgcgacca agcgctcgcg gccacggctg cttcttactt cgcagtgttc gctcatctcg
     5701 gccgagccgg ccgcatggtc gtactactga acgatgcaga cctggaacgc atcaatccct
     5761 ggccgttcaa catcaacggg ctcgcaacgt ccgagaaggt cgtactggtc caagacgacg
     5821 agagccacat cacagtcatg cacgagttag tacatacgat cccgcacgtt ttcctggacg
     5881 atggacaaca gccttgcggc ccgcccaact atcacaacag ggagctcaac acggcgaatg
     5941 ggttgcaact cacggt
//
LOCUS       DATMED010000098         5382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2762630, whole genome shotgun sequence.
ACCESSION   DATMED010000098 DATMED010000000
VERSION     DATMED010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762630"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..491)
                     /locus_tag="VNP73_02465"
     CDS             complement(129..491)
                     /locus_tag="VNP73_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL64812.1"
                     /translation="MSAVSVGSVVIDCNDFPTMFEFWRRALDYAPREDPEEDWVVLTD
                     PAGGGVNVSLQVVPEKRVGKNRLHLDLYTDEQDREVERLVGLGAVRHPRTPEVGEDFV
                     VLEDPEGNLFCVIQKGTG"
     gene            complement(488..841)
                     /locus_tag="VNP73_02470"
     CDS             complement(488..841)
                     /locus_tag="VNP73_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HWL64813.1"
                     /translation="MVTIADVRALAEKLPRSYEAVVRDRVKFRVGRIVYLAFSHDETM
                     MGFAFPKEEREVLIATEPDKFLMPKPRDQRYNWVVVRLEALDKAEMREIVLQAWRMVV
                     PKKVFAEFQEKQNIG"
     gene            complement(875..3028)
                     /locus_tag="VNP73_02475"
     CDS             complement(875..3028)
                     /locus_tag="VNP73_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWL64814.1"
                     /translation="MTEDNKQSEQIIGSGVRDVARSPYVRWLSLLASLVLAFIAADEM
                     VEYALAQIPLPREVKLPSSARVYDRNGELIATFRDEVTRYIINSKTLPPHVRRAVVAA
                     EDRDFYEHEGVSIKDTARAAWKNLTNTGVLQGGSTITQQYVKNAVLEDPSRTIERKFK
                     ELILSVKVERRYSKTRILDMYLNTVYFGRGAYGIEAASRVYFAKHASQLTLSEAAYLA
                     GLIPAPADYEGRRAGMERRSYVLKGMRDEGYITAREMRRADRTRLKLRTHETLRRGHQ
                     EAAYFLEWLRKDYLYPRFGDCLYRCGLKIHTTLDLGMQSEAEEAIDSILTEPSDPQAA
                     LVSMTPDGEVRAMVGGRDFTEVKRARGFNYATDGKRQAGSAFKPFTLLSAIEQDISTS
                     SRFSGESPKTIEDPACTGPDGVWMPENYGGSSFPTLDLTSATANSVNTIYAELVSEVG
                     PENVAEVVESFGFTGSARSGEVEPVCSLSLGTLDVSVLEMTRAFAGFAARGNLPEPVP
                     IRFVTDRSDECILGFRTGDDMKCRKRDEIAPRRAAEANDVDVLNQALQSVVEGGTATA
                     AQIGRPVAGKTGTTQDNRDAWFAGYTPQLATVVWMGYPIEAGPDKKFGTPDDVSPLMH
                     YCSDPAACRPVHGIEVTGGSFPATIWGLYMQAALVDTPIESFTVPVDLPDVVLNPPPP
                     PKPERDKGRKGWKWDDDDDHDDDPGNGNAYGRDDD"
     gene            3126..3488
                     /locus_tag="VNP73_02480"
     CDS             3126..3488
                     /locus_tag="VNP73_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64815.1"
                     /translation="MNLETLLTLAVTGLILVLALILRLLVVGMIRLVRVVTGRPALAE
                     RRSISDAVAKRSWNERMARVGGGFRWVGRGLKSVLDAVVALLAVIAAGFATGAAHLIE
                     RRSVSDGRSEAESDMQLV"
     gene            complement(3490..3894)
                     /locus_tag="VNP73_02485"
     CDS             complement(3490..3894)
                     /locus_tag="VNP73_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL64816.1"
                     /translation="MTEDLTPTPLTGILESVLYCTPETEAATRHFYAEVLGLRQVSQW
                     TYRLGDQLFLLFNAQETRVQEWPPPHGAAGSGHICFTVPPEGYAEWKDHLAANGTEVI
                     EEIDWSRGVLSFYFKDPAGNMLEIANGDMWPR"
     gene            complement(4089..4685)
                     /locus_tag="VNP73_02490"
     CDS             complement(4089..4685)
                     /locus_tag="VNP73_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HWL64817.1"
                     /translation="MDFRDPALVHLGLHASATGGERNDHFVEFYDSDRSVVDSARTFI
                     SMGLSEGESALVVASSGHRKAIERELEATVDLARARSKGLYSSFDAKETLALFMREGR
                     PDPVQFERVVEKLLARAMEGGRKVRVFGEMVAILWGQGNVAAAIELEELWNRVLQASP
                     FRLFCAYPMDVFEDGDPDSIGALFHTHSQVVVPRSEPV"
     gene            4984..5211
                     /locus_tag="VNP73_02495"
     CDS             4984..5211
                     /locus_tag="VNP73_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64818.1"
                     /translation="MDPRFKYVFRVLGRYEARLAGNEPHVTLNVLAGRDGHVVFCGTL
                     TMSEAEWDGLRNLFNSADPDAIEVESEVPTV"
BASE COUNT          969 a         1690 c         1659 g         1064 t
ORIGIN      
        1 tgctcgctcg cggatcccgg tcaacagacc gtccgggatc ttccacggca ccgaggcgta
       61 catatcgagg atcggttcct cggcgaagct gagcgcgagc tcgtcgggaa gatcgagtct
      121 gagcgcgctc atcccgttcc cttctggatg acgcagaaca ggttgccctc cggatcctcg
      181 agcacgacga aatcctcgcc gacctcgggg gtcctcggat ggcggactgc ccccagaccc
      241 acgagacgct cgacctcccg atcctgctcg tcggtataga gatcgagatg gagtcggttc
      301 ttccccaccc gcttctcggg gacgacctgg agcgagacgt tgacgccgcc gccggccgga
      361 tcggtaagca cgacccaatc ctcctccggg tcttccctcg gcgcatagtc gagagctcgg
      421 cgccagaact cgaacatggt gggaaagtcg ttgcagtcga tgaccaccga acccacgctt
      481 accgcgctca tccgatgttc tgtttttcct ggaactcggc gaagaccttc ttggggacga
      541 ccatccgcca tgcctgaaga acgatctcgc gcatctctgc cttgtccaac gcttccagcc
      601 gaaccaccac ccagttgtaa cgctgatcgc gcggcttcgg catcaagaac ttgtcgggct
      661 cggtggcgat gagaacttcg cgttcctcct tggggaacgc gaagcccatc atggtctcgt
      721 cgtgggagaa ggctagatag acgatccggc cgacccggaa tttcacccga tctcgaacga
      781 ccgcctcgta gctgcgggga agcttctcgg ctagtgcacg aacgtcagcg atcgtgacca
      841 tccaaggacc ctatgccggg ggccgcgagg agaactaatc gtcgtcgcgg ccgtaggcgt
      901 tcccgttgcc cggatcgtca tcgtgatcgt catcgtcatc ccacttccaa cctttcctgc
      961 ccttgtcccg ctcgggcttg ggtgggggag gcgggttcag aacgacgtcc ggaaggtcca
     1021 ccggcacggt gaaggactcg atgggcgtgt cgacgagggc ggcctgcatg tagagacccc
     1081 agatcgtcgc cgggaaggaa ccgccggtca cctcgatccc atgtacgggc cggcatgcgg
     1141 ccgggtcgct gcagtagtgc atcagcgggg agacatcgtc cggggtcccg aacttcttgt
     1201 cgggaccggc ttcgatcgga tagcccatcc acacgaccgt ggccaactgg ggtgtgtacc
     1261 cggcgaacca cgcgtcgcga ttgtcctggg tcgttcccgt ctttccggca accggacgtc
     1321 cgatctgagc ggccgtcgcg gttccaccct cgaccacgct ttgcagcgcc tgattgagga
     1381 cgtcgacgtc gttcgcctct gccgccctgc gaggcgcgat ctcgtcgcgt ttccggcact
     1441 tcatgtcgtc cccggttctg aagccgagga tgcactcgtc agaccggtcg gtgacgaaac
     1501 ggatcggcac gggctcgggc aggtttcctc gcgccgcgaa gccggcgaac gcacgcgtca
     1561 tctccagcac ggagacgtcg agcgttccca atgacagcga gcagacgggc tcgacctcgc
     1621 cgctgcgagc cgatcccgtg aatccgaaac tctcgacgac ctcggccaca ttctcggggc
     1681 caacctcgct caccagctct gcatagatcg tgtttacgga attggccgtt gcggacgtga
     1741 gatcgagggt cgggaaagag gagcccccgt agttctccgg catccacact ccatccgggc
     1801 cggtgcaagc gggatcctcg atcgtcttgg gactctcgcc cgagaaccgt gagctggtcg
     1861 agatgtcctg ttcgatcgcg ctcagtagcg tgaacggctt gaaggccgag cccgcttggc
     1921 gcttgccgtc ggtcgcgtag ttgaacccgc gcgctcgctt cacctcggtg aagtcgcggc
     1981 ccccgaccat cgcgcgcacc tcaccatccg gcgtcatcga gacgagcgcc gcttgcggat
     2041 cgcttggctc cgtcaagatc gaatcgatcg cctcctcggc ctcgctctgc atcccgagat
     2101 cgagtgtcgt gtggatcttg agaccgcagc ggtacaggca gtcgccgaag cgcgggtaga
     2161 ggtagtcctt ccggagccac tcgaggaagt aggcggcttc ctgatggccg cgcctcaaag
     2221 tctcgtgcgt tcgcagtttg aggcgtgttc ggtcggccct tcgcatctcg cgtgcagtga
     2281 tgtatccctc gtcccgcatc cccttgagta cgtaactcct tcgttccatg ccggcacggc
     2341 gaccttcgta gtcggcgggc gcagggatga gcccggcgag gtatgcggct tccgagagcg
     2401 tgagctgcga cgcgtgcttc gcgaagtaga cccgggaggc ggcctctatg ccgtaggcgc
     2461 cgcgaccgaa atagacggtg ttgaggtaca tgtccaggat ccgggtcttc gagtaccgcc
     2521 gctcgacctt caccgagagg atcagctcct tgaacttgcg ctcgatcgtc cggctcgggt
     2581 cctcgagaac ggcgtttttg acgtactgct gcgtgatcgt cgatccaccc tgaaggacac
     2641 cggtgttcgt caggttcttc cacgccgccc gcgcggtgtc cttgatcgag acaccctcgt
     2701 gctcgtagaa gtcgcggtcc tcggccgcaa ccacggcccg tcgcacgtgt gggggcaggg
     2761 tcttgctgtt gatgatgtag cgcgtgacct catcgcgaaa ggtagcgatc agctcaccgt
     2821 tgcggtcgta aacgcgcgcc gaggagggca gctttacttc tcgaggaagg gggatctgcg
     2881 ccagcgcgta ctcaaccatc tcatcggccg cgataaatgc gaggactaga gaggcgagca
     2941 ggcttaacca acgcacgtag ggggagcggg ccacgtcccg cacgcccgag ccgatgatct
     3001 gctcgctctg cttgttatct tcggtcatat atctccgagc tctgaatgtg cttcggccat
     3061 ccggcatgaa tacccagttg ctggggaagg atgcttgaaa tgcctgcgat gttgatctcg
     3121 aacgaatgaa cctagaaacc cttcttaccc ttgcggtaac gggattgatc ctcgtcctgg
     3181 ctctgatcct gcgtttactg gtggtgggga tgatccgcct ggtcagggtc gtgacggggc
     3241 gtccggctct cgcagagcgc aggtcgatct ccgacgcggt cgcaaagcgc agctggaacg
     3301 agcgcatggc gagagtcgga ggtgggttcc ggtgggtcgg acgcgggttg aagagcgttc
     3361 tcgatgccgt ggtggctctg ctggccgtca tcgccgcagg cttcgcgacg ggggctgctc
     3421 atctgatcga acgtcgctcg gtttcggatg gccgatcgga agccgagtcc gacatgcaac
     3481 tcgtttgatc tagcgcggcc acatatcgcc gttggcgatc tccaacatgt ttccggccgg
     3541 gtccttgaag tagaacgaca gcacgccccg gctccaatcg atctcctcga tgacctcggt
     3601 gccgttcgcc gccaagtgat ctttccactc ggcgtagccc tccggtggaa ccgtgaagca
     3661 gatgtgcccg cttccggcag ccccatgcgg aggaggccac tcctgcacgc gcgtctcctg
     3721 ggcgttgaac aggagaaaga gttgatcgcc gagcctgtag gtccactggc tcacttgacg
     3781 aaggcccaat acctcggcat agaagtgtcg cgtcgccgct tcggtttcgg gtgtgcaata
     3841 gaggactgac tcgaggatgc cggttagcgg agtcggagtg aggtcctcgg tcacttctta
     3901 gccgttcttg ctagctcgct tccgaatctg aatcatcggc tacctcgatc aggggtgagg
     3961 aacacaccgg gcacgccaag ggactgcctt cccgcaggta ggcagttttc tgacatctga
     4021 tacagaaagc ttcgatcatc ggcgtccctt cgaggcagcc ggcactctag ctgaggcgcc
     4081 gtggatctct agacgggctc cgagcgaggg acgaccactt gcgaatgcgt gtggaagagg
     4141 gcgccgatcg aatcgggatc accgtcttcg aacacgtcca tcggatacgc acaaaacagt
     4201 cgaaagggac tcgcctgcag cacgcggttc cagagttctt ccaactcgat cgctgcagcg
     4261 acgtttccct gcccccacaa gatcgcgacc atctcgccga agaccctgac ctttcttccg
     4321 ccctccatcg cgcgggcaag caacttctcg accacccgct cgaactggac cggatcgggc
     4381 cttccttccc gcatgaacag ggccaacgtt tcctttgcgt cgaacgagct gtaaagacct
     4441 ttcgagcgcg cccgtgcgag atcgaccgtc gcctccaact cgcgttcgat cgctttgcga
     4501 tgcccggagc ttgcgaccac cagggccgac tcaccttcgc tcagtcccat cgagatgaaa
     4561 gtgcgggccg aatcgacgac ggatcggtcg gagtcgtaga actcgacgaa atgatcgttg
     4621 cgctcacccc cggttgcaga ggcatgcagg ccgagatgaa cgagtgccgg gtcgcggaaa
     4681 tccatgggcg agtgccctct cctttgagta cttaagtccc aggacgacgc ttcatgatat
     4741 ttccagaagc cgaccgaggt aaaccggcgg tcgcgttggt tggaccgctt atgaccgggt
     4801 tgtctctgaa tcgcacgcga agagccgctc ggcaagttcc accgcgggat cggggccggg
     4861 aaatcggccg gtatcgagat acgtctacgt tcatcggggc cgccgagcgg gcgaaaacat
     4921 caagacgggg acgatcgctg gcatacgatc atgtcgaagg tcggcgattt ctgagggatg
     4981 acggtggatc ctcggttcaa gtacgtgttc cgagtgctcg gccgctacga ggccaggctc
     5041 gcagggaacg aaccgcatgt caccttgaat gtgctcgcgg gtcgcgacgg gcacgtcgtc
     5101 ttctgcggga ccctcacgat gtccgaggcc gagtgggatg gcctcaggaa cctattcaat
     5161 tctgccgacc cggacgcgat cgaggtcgag agcgaggttc ctacggtctg acgcctacca
     5221 ccccatgaag ccggagcgtg cccgcatgta attgtcgatc acctgatcgc tgatctccgc
     5281 gctctccgct cggaagacct cgccacccgt aagacgcgca agtttgtcca tgaactctcg
     5341 cagcccgggc gagtcctcga gcatgaagac gtttatcgag at
//
LOCUS       DATMED010000099         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5066382, whole genome shotgun sequence.
ACCESSION   DATMED010000099 DATMED010000000
VERSION     DATMED010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5066382"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..859
                     /locus_tag="VNP73_02500"
     CDS             32..859
                     /locus_tag="VNP73_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64819.1"
                     /translation="MPEATAPDGVNLHWQERGAGPTVLLSPYWAMHPSVFDPIEAVLA
                     EDFRVLRYDERGTGQSDRVGPYDMETGVSDLRAMCEAAGPVEAAICLIDSANRAVRVA
                     SSAPDLLRSVFCVGAAPFGVGALRDSESLLSSATVVRTYLQQLEADYRGAIRAALSGA
                     NSRLTEPEVRDRVQAQMDYVEAEAASTRARVWATDAHGEEPGRAIGSRLTVCLSNTMG
                     GPGSWFPTADEMEPVVKEVFPEAQIHWAEDGIVSAPREVAEVLKAALAQTETYHRQR"
     gene            856..1314
                     /locus_tag="VNP73_02505"
     CDS             856..1314
                     /locus_tag="VNP73_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWL64820.1"
                     /translation="MNTWILTGGVENFRIYTERGFDVIGMKEGRRRMAEGFEPGDEII
                     FYVSGLQAFGGIAKVTSGMFEDRTPIWPQGKKKKPEAYPWRVEAEPDIVLAEDEFVPA
                     EEMVGRLDHIAKWPAEHWHLAFQGQLRTISESDAKILRQRIESARVSTAA"
     gene            1699..2196
                     /locus_tag="VNP73_02510"
     CDS             1699..2196
                     /locus_tag="VNP73_02510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWL64821.1"
                     /translation="MASSALAGNGGGVGTGGGGNGGGDSSGGNHAYVFPLPTRHSYGD
                     GFGAGRDHQGQDVFAKCGSKLVAVRSGRVQTVDFQSSAGNYIVIDGNNTGIDTAYMHL
                     KKKAIPREGARVRQGEKIGEVGDTGNASGCHLHFEKWTSPGYYEGGKASRSVTKALKS
                     WDKYS"
     gene            complement(2223..2642)
                     /locus_tag="VNP73_02515"
     CDS             complement(2223..2642)
                     /locus_tag="VNP73_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64822.1"
                     /translation="MNKRRTAASCVIASLVVVSILATPVIAGFKSGQYVGTTSQLDQV
                     AQPLNVGFDVPKNKNKVNIVYFEFLNVDCNKVTQVAGETVKLKKSGKFKLKDADLPFS
                     SGYIKGKFKGKKASGTAEFPDRCPSAGVIDWEAEKDS"
     gene            complement(2737..>3546)
                     /locus_tag="VNP73_02520"
     CDS             complement(2737..>3546)
                     /locus_tag="VNP73_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWL64823.1"
                     /translation="NNFPSYAADVTKFVNDNDTLSGLNEDYDITGTIEEEAKKLPEKI
                     GDATQTLSDIGVGLVRSIFALVTILILSIFMVSGGARWIEQFLDNQEPEHAERLRRAF
                     EHIASAIGGYVGGALLQATFAGVVSFVVLSILGAPFAGALALIMAFGDLIPVVGATIA
                     ATLVGIVLLFVNFPVALIVWIIFAIVYQQIENYVIQPQIQGRAVAVQPIVILVAVLFG
                     STLFGVVGALLAIPAAASIQIAVREWNDYRRAVNLGVEKPAASSIIQPEPG"
BASE COUNT          656 a         1131 c         1137 g          622 t
ORIGIN      
        1 taaccggctc gtccctacct agactcgggg catgcccgag gcaacggccc cagacggcgt
       61 caacctccac tggcaggagc gaggcgcggg gccgacggtc ctcctcagcc cgtactgggc
      121 gatgcaccca tcggtcttcg acccgatcga ggcggtgttg gccgaagact tccgggtcct
      181 ccgctatgac gaacgcggga ccggtcaatc cgatcgcgtc gggccatacg acatggagac
      241 tggcgtctcg gacctgcgtg cgatgtgcga ggccgcgggc ccggtcgagg ctgccatctg
      301 tttgatcgac tcggccaacc gcgccgttcg ggttgcctcc tcggcgcctg atcttcttcg
      361 ctccgtcttc tgcgtgggcg ccgccccgtt cggtgttggg gcgcttcgtg actcggaatc
      421 cctgctctcc tcggcgacgg tggtacggac ctacctccag cagctcgagg ccgattatcg
      481 gggggcgatc cgcgccgccc tctcaggcgc gaactcgcgc ctcaccgagc cagaagtcag
      541 ggaccgggtt caggctcaga tggactacgt agaggccgag gcggcatcca cccgcgccag
      601 ggtgtgggca acggacgccc acggagagga gcctggccgg gcgatcggaa gccggctcac
      661 cgtctgcctg tcgaacacga tgggtggccc gggcagttgg tttcccaccg cggacgagat
      721 ggagccggtc gtaaaagagg tgttccccga ggcgcaaatc cactgggctg aggacggaat
      781 cgtctcggca ccgcgggagg tggccgaggt cctcaaggct gccctcgcgc aaaccgagac
      841 gtatcatcgg cagcgatgaa cacctggatc ctcaccggcg gcgtcgagaa cttccgcatc
      901 tacacggagc gcgggttcga cgtgatcggc atgaaggagg gtcgccggcg catggccgag
      961 ggcttcgaac cgggtgacga gatcatcttc tacgtctccg gcctccaggc gttcggcggc
     1021 atcgccaaag tgacatccgg catgttcgag gatcgcacgc cgatctggcc gcagggaaag
     1081 aagaagaagc cagaggccta tccgtggcgt gtcgaggccg agcccgacat cgttctcgcc
     1141 gaggatgagt tcgtgcccgc agaagagatg gtcgggcgcc tcgaccacat cgcgaagtgg
     1201 cccgccgagc actggcacct cgcgttccaa ggtcagcttc ggacgatttc cgagagcgac
     1261 gcgaagatct tgcgccagcg catcgagtcc gctcgcgtca gcacggctgc gtaagagaat
     1321 gaaacagggc gccccgtaag ggacgccctg tctcggcatc ggaggtgccc cgtggctcag
     1381 gtctggaccg gggtaagggc ccagactctc ctcgggttca gaaatgtctt cgacgcccgc
     1441 gcaccacccc tttcgcggcc tcgcccgttc tggacgttca tacgacggcc ggcggtggga
     1501 gacgagcgcg gcgcgtcgaa ccatggactc gaggagacac cgcgagccgc cgatacgacc
     1561 cttagtctgc tatgagtatc cgctaacaaa atgcctagca gccacacaaa gaacccagtc
     1621 ccggcctcac aaaagcacca ctcgaaggcc agattcactg ccattgcagc ggcctccctg
     1681 cttggagccg cctgtctttt ggcctcctcc gccctcgccg gcaacggcgg cggcgtgggc
     1741 accggcggtg gcggcaacgg cgggggcgac tcgagcggag gcaatcacgc gtacgtgttc
     1801 ccgcttccga cccgccactc gtacggcgac gggttcggcg ccggacgcga ccatcagggc
     1861 caggacgtgt ttgccaaatg cggctcgaag ctcgtggcgg tccggtccgg acgggttcag
     1921 accgtcgact tccaaagctc cgcgggtaac tacatcgtca tcgacggcaa caacacgggc
     1981 atcgacacgg cgtacatgca cttgaagaag aaggcgatcc cgcgcgaggg agcgagggtt
     2041 cgccaaggcg agaagatcgg cgaggtcggc gacaccggca acgcgtcagg ctgccacctt
     2101 catttcgaaa agtggacctc cccgggctac tacgagggcg gcaaggcctc ccgttcggtc
     2161 acgaaggccc tcaagtcctg ggacaagtac tcctgaggct cgactgcggt taggtacgcc
     2221 gatcaggagt ccttctcggc ctcccagtcg atcacgcctg ccgacgggca ccggtcgggg
     2281 aactctgcgg taccgcttgc cttctttccc ttgaacttgc ccttgatata gccggacgag
     2341 aaaggcaggt cggcgtcttt gagcttgaac ttgccgctct tcttcagctt cacggtctcg
     2401 cccgctacct gggtgacctt gttgcagtcc acgttcagga actcgaagta gacgatattc
     2461 accttgttct tgttcttcgg cacgtcaaag ccgacgttca ggggctgcgc gacctgatcg
     2521 agttgcgagg tcgtcccgac gtactgcccc gacttgaacc cagcgatgac gggggttgcc
     2581 aggattgaca ccaccactag tgaggcgatt acgcacgagg cagcagtccg tcgcttattc
     2641 atctttcctc cttgaaaaag ccgtgagccg cgcttaatcg tacggcgaga tcgtgcgcct
     2701 gtcaatcgtt tcggagcttg gtccaggccg tctgggctag cccggctcgg gctggatgat
     2761 cgaagaggcg gcgggtttct cgactcccag gtttaccgcc cgccgatagt cgttccactc
     2821 ccggacggcg atctggatgg aagccgccgc agggatcgcg aggagcgccc ccaccacccc
     2881 gaagagcgtt gatccgaaca gcaccgcgac caggatgacg atcggctgaa ccgcaaccgc
     2941 acggccctgg atttgtggct ggatcacgta gttctcgatc tgctgataga cgatcgcgaa
     3001 gatgatccag acgatgagtg cgacggggaa gttgacgaac aggagcacga ttcccaccag
     3061 cgtcgcggca atcgtggcgc ctacgacagg gatcagatcg ccgaaggcca tgatgagagc
     3121 aagggcgccg gcgaagggag ctccgaggat cgagagcacg acgaatgaga ccacgcccgc
     3181 gaacgtcgcc tgaaggaggg cgcccccgac gtagccgccg atcgcgctgg cgatgtgctc
     3241 gaacgcccta cgcaggcgct ccgcgtgctc gggctcttgg ttatccagga actgctcgat
     3301 ccaccttgcc cctcccgaga ccatgaagat cgaaagaatc aggattgtca ccagcgcgaa
     3361 gatcgacctg accaggccga cgccgatgtc ggagagcgtc tgcgtcgcat ccccgatctt
     3421 ctcgggcagc ttcttcgcct cttcttcgat cgtgccggtg atgtcgtagt cctcgttcag
     3481 acccgagagg gtgtcgttgt cgttgacgaa cttggttacg tcagcggcgt acgaggggaa
     3541 gttgtt
//
LOCUS       DATMED010000100         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3522835, whole genome shotgun sequence.
ACCESSION   DATMED010000100 DATMED010000000
VERSION     DATMED010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3522835"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="VNP73_02525"
     CDS             <1..1703
                     /locus_tag="VNP73_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL64824.1"
                     /translation="LREDARRWLRHRGDHDYGCFSRSIRSFSASMAKWLPMIILASAQ
                     FVMVLDSSVMNVAISQIVDDLDTSIQGVQTAITLYTLVMAAFMLLGAKLGDILGRNRA
                     FAIGLGIYGIGSLTTALSPSLAVLLVGWSGIEGFGAVLVVPAIASLTAASYQGKDRAL
                     AYALLGAVAAIAVAAGPLIGGWVTTEFTWRYVFAAETVVVIAILFLRKRIAQAPAAER
                     RPRLDGVGVALSSFGLGLMVFAILRSSVWGFVQPRNPPTINGTEITPLGFSPVPFLVL
                     TGLAFLAAFVVWERRRSRLGEDQLLDTTLLRGPQLRAGLSTLVGQQLVLMGTFFVIPV
                     YLQVVLGLDAFETGKRLLPLSVAMLVFAILGPRVAARRSPRTVAQIGLVAVSVGAIVM
                     LATLDVTLNDTGFKVALGLIGAGAGLLASQLGNVIMSSVPPSKSNEGGGLQGTAQNLG
                     SSLGTAIIGAVLLASLATGFGERVTDNTAIPAEARETIVAKTESGIDIVPVADVEQAA
                     LAGGLTSDEAQAVATDYGDAQLEALRLALGAVALAALVSLWFTGRLPTESLAADEVKA
                     "
     gene            complement(1815..2384)
                     /locus_tag="VNP73_02530"
     CDS             complement(1815..2384)
                     /locus_tag="VNP73_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWL64825.1"
                     /translation="MTTTLTPIPTATEIPAGTWKTDPAHTRVGFAVKHMGVSTVRGEF
                     REFDGTFEVGEDLSSSRAYGTVQAASVDTNQPQRDEHLRSADFFGAEENPELRFESTS
                     IEAIDEDSFRVVGNLAMNGVTNEVELLAEITGTDIGPAGEERLGLEVTGQVSRRDFKM
                     KFDAALGSGNAVVGDKVKLALDIAVIKGS"
     gene            2582..>2824
                     /locus_tag="VNP73_02535"
     CDS             2582..>2824
                     /locus_tag="VNP73_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWL64826.1"
                     /translation="MRELDEELQSAHDLTIGEFDVLRALFTAPDHRRRMCDLAEAVVL
                     TASGLSRRVDRLERAGWVSRERSNEDGRSVETVLTDD"
BASE COUNT          434 a          878 c          925 g          587 t
ORIGIN      
        1 cattgcgtga ggatgcccga cggtggctga ggcaccgcgg agatcatgac tatggctgtt
       61 tctctcggag tatccgctcg ttctcggcgt cgatggccaa gtggctaccg atgatcatcc
      121 tggcgtcggc ccagttcgtc atggtgctcg attcgagtgt catgaacgtc gcgatctctc
      181 agatcgtcga cgatctcgac acgtcgatcc agggcgtgca aacggcgatc accttgtaca
      241 cgctcgtgat ggccgctttc atgctcctgg gagcgaagct cggcgacatc ctagggcgga
      301 atcgcgcgtt cgcaatcggt ctcggcatat atggaatcgg gtcgctcacg accgctctga
      361 gtccgagcct tgccgtcttg ctcgtcggtt ggtcggggat cgaggggttc ggggctgtcc
      421 tggtcgtgcc cgcgattgct tcgctcacag ccgccagtta tcaaggcaag gaccgagctc
      481 ttgcctacgc gctgctcggt gctgtcgcag ctatcgccgt ggccgccggt cccctcatcg
      541 ggggttgggt cacgacggag ttcacgtggc gctatgtgtt tgccgcggaa accgtcgtgg
      601 tgatcgccat cttgtttctc cgcaagcgga tcgcgcaggc tcctgcggcc gagcgccgcc
      661 cgcgactcga cggcgtaggc gttgcccttt cgtcgttcgg actggggctg atggtgttcg
      721 cgatcctccg cagcagcgtc tgggggttcg tccagccacg caacccgccg acgatcaacg
      781 gaaccgaaat caccccgctg gggttctcgc cggtgccgtt cttggtcctt accggtctag
      841 ctttccttgc cgccttcgtg gtttgggagc gaaggcggtc tcgattgggg gaagaccagc
      901 ttctcgacac cacactgctc cgaggtcccc agctgcgggc cggactctcg acactggtgg
      961 ggcagcagct cgtcctcatg ggcacgtttt tcgtcattcc cgtctacctg caggtggtac
     1021 tgggcctcga cgcgtttgag accggcaagc ggctgttgcc gctgtcggtg gcgatgctcg
     1081 tgttcgcgat tctcggacca cgcgtcgccg cacggcgctc gccgcgaacc gtggcgcaaa
     1141 tcgggttggt cgctgtgagc gttggcgcga tcgtgatgtt ggcaacgctg gacgtgaccc
     1201 tcaacgacac cggcttcaag gtggccttgg gcctgatcgg tgccggagcc ggcctcctcg
     1261 cttctcagct tggcaacgtg atcatgtcct cggtcccgcc ttcgaagagc aatgagggag
     1321 gtgggcttca gggcaccgct cagaacctgg gatcatcgct tggcacggcg atcatcggcg
     1381 cggttctgct cgcgtccctg gcaacgggct tcggggagcg tgttacggac aacaccgcca
     1441 tcccagcaga ggcgcgtgag acgatcgtgg cgaagaccga gtcggggatc gacatcgtcc
     1501 ccgtcgccga tgtcgaacag gcagcgctgg ctggcggact cacgtcagac gaggcgcaag
     1561 cggtcgccac cgattacgga gacgcgcagt tggaggcgtt gcgcctagct ctcggcgctg
     1621 tcgcgttggc ggccctcgta tccctctggt tcaccgggcg gcttccgacg gagtcgctcg
     1681 ccgcggatga ggtcaaggcc tgagagttga gcccctagcg gaggcaattc ctggcttact
     1741 cctgagctcg acctggtcaa gagacccggc gacataatcg gccgccgccg ggccttagcc
     1801 tgcgagttac gcgcctacga acccttgatc accgcgatgt cgagtgcgag cttgaccttg
     1861 tcgcccacga cggcgttgcc gcttcctagc gcggcatcga acttcatctt gaagtcccga
     1921 cgcgagacct gaccggtgac ctcgaggccg aggcgctctt cgcctgccgg gccgatgtcg
     1981 gtccccgtga tctctgcgag cagctcgact tcgttcgtga cgccgttcat cgccaggttg
     2041 ccgacgacgc gaaagctgtc ctcgtcgatc gcctcgatgc tcgtcgactc aaaccgcagc
     2101 tcggggttct cctcggcgcc gaagaagtcg gcggagcgga gatgctcgtc gcgctggggc
     2161 tggttggtgt caaccgatgc cgcctggacc gtgccatacg cgcgggagga gctcaggtcc
     2221 tcgccgacct cgaaagtgcc atcgaactcg cgaaactcgc cgcggacggt gctgacgccc
     2281 atgtgcttga cagcgaatcc aacccgcgtg tgtgcgggat cggtcttcca ggtacctgcg
     2341 gggatctcag ttgcggtggg gatgggggtg agggtggtgg tcatcgtggt tcctttcggg
     2401 aagttatttg cttgcgcaat caaatatagc acgaagcttg cgcccgcaag taaatctggc
     2461 taagcttcct cccatggccc tagatacagc cactcccgga acgagcgcgg gtgcgcctct
     2521 cgcgtttgac tcagccggat ttgcggcatg gcgcggtctg attttttcga gctctcgcct
     2581 gatgcgggag ctcgacgagg agcttcaaag cgcgcatgac ctaaccatcg gagagttcga
     2641 cgttctacgg gccctcttca ctgcgcctga tcaccgacga cgtatgtgtg acctcgccga
     2701 ggctgtagtg ctgaccgcga gcggcctctc gcggcgtgtc gaccggctgg agcgtgcggg
     2761 ctgggtcagc cgcgagcgct ctaacgagga tggacggagc gtcgagacgg ttctcaccga
     2821 cgat
//
LOCUS       DATMED010000101         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3523077, whole genome shotgun sequence.
ACCESSION   DATMED010000101 DATMED010000000
VERSION     DATMED010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3523077"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..1724)
                     /locus_tag="VNP73_02540"
     CDS             complement(435..1724)
                     /locus_tag="VNP73_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWL64827.1"
                     /translation="MQRRLRAAPLIALVLAGSIWLPHTPIAAGNLSPSEWVIGPQQRV
                     VILTFDGRARGKALARVLKDLADRDARASFFLPGSYVAKHPDKARLILAAGHKLGNAG
                     WGKERFTKISDTAIRDSIRKAQAALNKVNVFPRPFLRVPNGARDVRVLRAAGSLGYRS
                     VRWTYHPGSGSVKDIVRKTVNGAQAGSVISLDLWRSGHRAAVGQIVDGLKERGFAFKS
                     ISALENVQPIRWDVTLRAGSSGPEVQFLQKTLGEITYPVGRKDGSFGYSTLQAVYAFE
                     KVHRMTRDGVVTPQQMTDIAIAQRPRAPKRGPKTFVDIDVSRQVLFEVKKKRVIHTLP
                     VSSGNEETYTTDDGETKQAHTPRGNFSVLRKIAGRRESDLGVLWWPNYFYGGFAVHGS
                     DSVPTYPASHGCVRIPRYLEQEFFNRNPVGRPVFVHD"
     gene            1871..>2408
                     /locus_tag="VNP73_02545"
     CDS             1871..>2408
                     /locus_tag="VNP73_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64828.1"
                     /translation="MRIHSTRTVAIVACAALLLGAFVAAPAEAKKKKKKKKAPVAAVC
                     APYAPGEKGGDQPVTVVTDASTAEAPAEVTISTAPGLGFSSEQGEGNPDEGATSHTYA
                     NVQVDSANPSAGLFVSIEFTPVFDYDLWLRDSNGTALAYSAGSSPPTPLTDGTGNGGH
                     SDVGSENIDGYLTADCAGF"
BASE COUNT          471 a          760 c          749 g          428 t
ORIGIN      
        1 caactttgtt gacagtcgat atctcgacgc gatcactctc gaacccgggc agcgaccaaa
       61 gcctccctgc agatcgatgc gacgcggcgc taccgtctcc ggcccaatag caagcggata
      121 gcaatctccg acgccaggac gatggcatgc ctgggatggc gtagacgcgt ggatgcggcc
      181 ggagcaacct gcccgaactt acataccaac tcagggaacg gtcagataag ccggccgctc
      241 gtgcctgcgc aagggtaaaa agtccctgct gtcgttctgc catctccaag caccgaagga
      301 aatgcgaatg aggcatctcc ccacagaaat cgaactgatg ccttcggtca gtgcctcaca
      361 ccacaatcga cattgttgtc acttacgaaa tatgtaagtc acacccaagt cggcgggggg
      421 ggcgccccgg ggggtcagtc gtggacgaag accgggcggc ctacggggtt gcggttgaaa
      481 aactcctgct cgaggtagcg agggatgcgc acgcagccgt ggctggcggg ataggtcggg
      541 accgaatccg agccgtggac ggcgaagccg ccgtagaagt agttcggcca ccacaggacg
      601 ccgagatcgc tctccctgcg ccccgcgatc ttccgcagca ccgagaagtt gcccctcggg
      661 gtgtgggctt gcttggtctc gccgtcgtcg gtcgtgtacg tctcctcgtt gccgctggat
      721 accggcaacg tgtggatcac gcgcttcttc ttgacctcga acagcacttg ccgcgagacg
      781 tcgatgtcca cgaacgtctt cgggccccgt ttcggagcgc ggggtctctg agcgatcgcg
      841 atatctgtca tctgttgcgg cgtcacgacc ccgtctcggg tcatgcggtg aaccttttcg
      901 aacgcgtaaa cggcctggag cgtggagtac ccgaacgatc catccttccg acccaccggg
      961 tacgtgatct ctccaagagt cttctggagg aactgaacct cgggtccgct ggaaccagca
     1021 cggagagtca cgtcccaacg gatcggctgc acgttctcga gcgccgagat cgacttgaag
     1081 gcgaatccgc gctccttgag cccatcgacg atctggccaa ccgcggcgcg gtgacccgaa
     1141 cgccatagat cgagggagat gacggatccc gcctgtgccc cattaaccgt cttgcgcacg
     1201 atgtctttta cagagccgga ccccgggtgg tacgtccacc gaaccgaccg atagccgagc
     1261 gatccggcgg ctcggagaac gcgcacgtcg cgcgccccat ttggaaccct caggaacgga
     1321 cgagggaaga cgttcacctt gttcaaggcg gcttgagctt tcctgatcga gtccctgatc
     1381 gcggtgtccg agatcttcgt gaaccgctct ttgccccagc cggcattgcc cagtttgtgg
     1441 ccggcggcaa ggatcaggcg ggccttgtcg ggatgcttgg ctacatatga cccgggcagg
     1501 aagaaggacg ctcgagcgtc tcgatcggcg agatccttga gcaccctggc cagcgcctta
     1561 cctcgggcgc gaccgtcgaa ggtgaggatc accaccctct gctgagggcc gatcacccac
     1621 tcggaaggcg acaggttgcc tgccgcgatt ggggtgtggg gcagccagat cgatccagcg
     1681 agtaccagag cgatcagagg ggccgcccgc agccggcgtt gcacgggccg taccctaccg
     1741 ggcttcgcga agaatcccga ccacgcctag tgagatttcg gggccgttga gtcgcaggaa
     1801 ggccttctgg ggtggcgaat cccttctcta ccagcagatc tagagaaaaa gcatgaaaga
     1861 ggaggttggc atgaggatcc actcaactcg caccgttgcg atcgttgctt gtgcggcgct
     1921 actactcggc gcgttcgtcg ccgctccggc cgaagccaag aagaagaaaa agaagaagaa
     1981 ggctcccgtt gcagccgtgt gtgcccccta cgccccaggc gagaagggtg gggaccagcc
     2041 cgtcacggtc gtgaccgacg cttcgaccgc tgaagccccc gctgaggtga ctatttcgac
     2101 cgcccccggt ctcggcttct ccagcgagca gggcgaaggg aaccccgacg agggagccac
     2161 cagccacact tacgcgaacg ttcaggtcga ctcggcgaac ccgagcgccg gcctcttcgt
     2221 gtcgatcgag ttcacaccgg tcttcgacta cgacctgtgg ctgagggact cgaacgggac
     2281 ggctctcgcg tactccgcgg gcagctcccc tccgacgcct ctgacggacg gaaccgggaa
     2341 cggtggccac agtgatgttg gttccgagaa catcgacggt tacctcaccg ccgattgcgc
     2401 gggcttca
//
LOCUS       DATMED010000102         5744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3524272, whole genome shotgun sequence.
ACCESSION   DATMED010000102 DATMED010000000
VERSION     DATMED010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3524272"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VNP73_02550"
     CDS             complement(<1..183)
                     /locus_tag="VNP73_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HWL64829.1"
                     /translation="MPEFRPFAGIRYSSQEELKDLSCPPYDVISAAEQERLHDRHPHN
                     AIRLELARPDSTADNKY"
     gene            193..981
                     /locus_tag="VNP73_02555"
     CDS             193..981
                     /locus_tag="VNP73_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HWL64830.1"
                     /translation="MIRERLAQLDGLVCDLDGVVYRGDEPVPGAAEAIENLRQRGIRV
                     VFCTNNSRSTVGQYVDKLAGVGVPAEPDEIVTSAIVTAEVLKERGFAGKTAIVVGGDG
                     LTEALGDVCVSVKSDPDVTKADLVVVGWDPSFDYAILTRAANAVRSGAILIASNEDAT
                     FPSPEGLLPGAGAMVAAIERASGRRAEVMGKPHPPMMKVVADRLHGCKSIAIVGDRPD
                     TDLAGARAMGWTSILVLSGVTTPADAEHVTPVPDLVLDSLAELG"
     gene            complement(978..1745)
                     /locus_tag="VNP73_02560"
     CDS             complement(978..1745)
                     /locus_tag="VNP73_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWL64831.1"
                     /translation="MIRSKAIILALVFVVAAPTAAVAQEPQVEPRDTVTVAASKDVDV
                     EPDIGTVSFGIRVNANDAAGAADKLTNRTRRVIDALKTIGFNSDEIDTYDVELARTCL
                     DRCRDPNPKDDKIPEPVMGYRGSSGLRVETSQLNRLGEVIDAAVDAGANNVRGISFDV
                     KDRSEAVKEALRQAMVFATEKAQILAETGGRQLGPALQILEGRTRAPEPADFDGALGG
                     RVSGGGSSGGGGGDNPFPIEPPTLSASARVTVTYELL"
     gene            complement(1810..2589)
                     /locus_tag="VNP73_02565"
     CDS             complement(1810..2589)
                     /locus_tag="VNP73_02565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWL64832.1"
                     /translation="MSQLIPISDLNPTRRFAVVTVVLIALNVVAFVQTPQFGLGTRST
                     VYFFENAPVPCQLEDSCPNDEILPGLRIPERNFGSFLMSIVVSTFLHGGPIHLAGNML
                     FLWVFGNNIEDRLGRVKYILFYLLGGVAAAFAHIYWTLARAPEACTNADPSACIPAVG
                     ASGAVAAIMGAYILLFPRARVNVLVAIFVLWTVIQLSAFAVLGLWFLYQFLIGSQEFS
                     GATEVAWMAHVGGFVYGVVVMFFLGGRPHKPPEVWRPEWGY"
     gene            2644..3054
                     /locus_tag="VNP73_02570"
     CDS             2644..3054
                     /locus_tag="VNP73_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64833.1"
                     /translation="MFDELRRVALFTSGVAELTRHQAEKIVKDFVKSGELRRKQASGA
                     VKELMQTSKAARTELARLVRSEIQNQIEGLGLATQRDLERLERRVARVEGDRKKTAAR
                     KTSSSGSKTTARKTSKAKKSTTRKKPDPARKTST"
     gene            3068..3235
                     /locus_tag="VNP73_02575"
     CDS             3068..3235
                     /locus_tag="VNP73_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64834.1"
                     /translation="MERPGANADPAVLDEWARRQLETAAVSDDATRLKILDELHEAFE
                     SGLAEDSAPRS"
     gene            3207..3998
                     /locus_tag="VNP73_02580"
     CDS             3207..3998
                     /locus_tag="VNP73_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWL64835.1"
                     /translation="MPRTLRLDLELVRRGLIGSRAAARRAIEEGRVTVRGFPAERASS
                     LVTADDPIALVGDPAPFVSRGGEKLDAALQVFDVVVRARRWLDAGASTGGFTDRLLQG
                     GAEEVVAIDVGYGQFAWELRNDPRVTLIERKNVRTLERSDLPFSPDGVVADLSFISLT
                     LVIGALAEVAANHADHLLLVKPQFEVGRTDVDKGVVRDPALWRAAIERVVVSAREHDL
                     ELRDVSLAHPPGPAGNREFFVHLTRGGQSGTDAIEREVERAAGEI"
     gene            3988..4785
                     /locus_tag="VNP73_02585"
     CDS             3988..4785
                     /locus_tag="VNP73_02585"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWL64836.1"
                     /translation="MRFERVAIVAHASKSLAREAETTLKEWLAERGIELSGEQPDLVI
                     SLGGDGTMLRAAKVAHAADAPLLGVNLGRVGYLTEIEMSQEMESLEKVFAGDYAIEER
                     MMLDCRVEIASEIHPFLGLNEVLLERSSRHRLVNLEVWVGEEQLGSFSADGVIVATPT
                     GSTAYALSAGGPIVSPRAECLVVVPVSAHMILSRPFVFAADETVTLKVGSGSSQGATL
                     VVDGAAGVEVPSGNAVDVGRNPRPLRLVKLAGPGFIERLRMKLGLPG"
     gene            4814..>5744
                     /locus_tag="VNP73_02590"
     CDS             4814..>5744
                     /locus_tag="VNP73_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWL64837.1"
                     /translation="MLVELVVAGLGAIDRAEVKLGPGCTALTGETGAGKTLLVAAVGL
                     LAGARGDRGLIREGQAEASVEGRFMVPRTHPAALLVAELGFQADDAADPVEVVLTRSI
                     ATSGSRARVNGRLATLTVLSELGGLLIEIASQHEHQRLASTRSQRALLDAFGGPDVIE
                     LAGKVEAAVRAAVAAARELEELEASRRERERELDVLRYEVAEIEGAALQEGESDDLKT
                     VAARLEHAEEIAGASRDISRLLTGESGVTDSLVQAQRVAEKAASLDGSFGTVAERLQA
                     LVVEADDVAQDVVRREVAPDPAALAATRERLDQI"
BASE COUNT         1069 a         1681 c         1986 g         1008 t
ORIGIN      
        1 gtacttgttg tcggccgtgg agtcggggcg agcgagctcg agtctgatcg cgttgtgcgg
       61 atgtcggtcg tgcagacgct cctgctccgc ggcagagatc acgtcgtacg gtgggcagct
      121 gaggtccttc agctcttcct gagaggaata tcggatgccc gcgaaaggtc tgaactcagg
      181 catgggaggc gcatgatacg ggagcgtctc gcccagctcg acggactggt ctgtgacctg
      241 gatggagtcg tatatcgcgg ggacgagccg gtccccggag ccgccgaggc gatcgagaac
      301 cttcggcagc gcggcatccg ggtcgtcttc tgtacgaaca actcacgatc gacggtgggg
      361 cagtacgtgg acaagctggc gggggtcggg gtgccggcgg agcccgatga gatcgtcacg
      421 tcggcgatcg tcacggccga ggtgttgaag gagcgtgggt tcgcgggaaa gacggccatc
      481 gtcgtcggag gggacggact cacggaggcg ctcggcgacg tctgcgtgag cgtcaagagc
      541 gacccggacg tgaccaaggc ggacctcgtc gtggtcggtt gggatccttc gttcgactat
      601 gcgatcttga cccgcgccgc caacgcggtc cgatcgggag ccatcttgat cgcttccaac
      661 gaggacgcga ccttcccgtc gcccgaagga ctgcttccgg gggcgggtgc gatggtcgcg
      721 gcgatagagc gcgcgtccgg gcgccgggcc gaggtgatgg gcaagccgca tcccccgatg
      781 atgaaggtcg tcgcggatcg actccacggg tgcaagtcga tcgcgatagt gggggaccgc
      841 cccgacaccg atctggccgg agcgcgcgcg atgggatgga cttcgatcct cgtgctctcg
      901 ggcgtcacga cgccagccga cgccgagcac gtcacgcccg tgccggacct cgtcctggac
      961 agcctcgcgg agctgggcta gagcagttcg taggtcacgg tcacccgggc actggccgac
     1021 aaggtcggag gttcgatcgg gaacggattg tcgcctccac caccaccgct gctgccgcct
     1081 ccggagaccc tgccgccgag agcaccatcg aagtcggcgg gctcgggggc tctcgttctt
     1141 ccttcgagta tctggagcgc gggtccgagt tggcggccgc cggtctcggc caggatctgt
     1201 gctttctcgg tcgcgaacac catggcttga cgaagggctt cctttacggc ttcgcttcgg
     1261 tccttgacgt cgaatgagat gccgcgcacg ttgttcgcac cggcatccac ggcggcgtcg
     1321 atgacttcgc ccagacggtt gagttgcgaa gtctcgactc tgaggccgga ggaccctcgg
     1381 taacccatca ccggctccgg gatcttgtcg tccttggggt tcggatcgcg gcagcgatca
     1441 agacaggtgc gtgcgagctc gacgtcgtac gtgtcgatct cgtcggaatt gaagccgatg
     1501 gttttcagtg cgtcgatcac ccgccgcgtt cgattagtca gtttgtccgc ggcaccggcg
     1561 gcatcattag cgttaacgcg gatcccgaaa ctaacggtcc cgatgtccgg ctcgacgtca
     1621 acgtccttag acgccgccac ggttaccgtg tcgcgaggct caacctgtgg ttcttgagcg
     1681 accgccgcgg tcggtgcggc gacaacgaag acgagagcaa gaatgatggc tttggatctg
     1741 atcacgtcga cccccctttg tagtgccgat gcaccttcga ctggggggtc gactattttg
     1801 ttcccgaggt cagtaacccc attcggggcg ccacacctcg ggaggcttgt gtggacgccc
     1861 cccgagaaag aacatcacga cgacgccgta aacgaaaccc cccacatggg ccatccacgc
     1921 gacttcggtc gctcccgaaa actcctgcga gccgatcagg aattggtaca ggaaccacaa
     1981 tccgagcacg gcgaacgcgg acagctggat caccgtccat aggacgaaga tcgccacgag
     2041 gacattcact cgcgcgcgtg ggaagagaag gatgtacgcg cccatgatgg ccgcgaccgc
     2101 ccccgaagct ccaaccgcgg ggatacaggc agatggatcg gcgttcgtgc acgcttcggg
     2161 agcacgtgcg agcgtccaat agatgtgcgc gaatgcggct gcgacgcccc ccagcagata
     2221 gaacaggatg tacttcacgc gaccgagccg gtcttcgatg ttgttgccga agacccacaa
     2281 gaagagcatg ttgccggcca ggtggatcgg gccaccgtgg aggaaggtcg aaacgacgat
     2341 cgacatcaag aacgatccga agttcctctc ggggatcctt agcccgggaa ggatctcatc
     2401 gttagggcac gagtcctcca gttgacaagg caccggtgcg ttctcgaaga agtagacggt
     2461 ggaccgcgtt cccagcccga actgcggtgt ctgcacgaac gcgacgacgt tcaaggcgat
     2521 gagcacgacg gtgacaacag cgaatcgccg agtcggattt aagtccgaga tcgggatgag
     2581 ctgcgacata cgcggcagta tagggtcggg tgatcgcgtg ctaattgttc agaggaggtc
     2641 ctgatgttcg acgaactcag acgcgttgca ttgttcacat ccggtgtcgc cgagctaacg
     2701 cggcatcagg cggaaaagat cgtcaaagac ttcgtgaaga gcggcgagct tcgacgcaag
     2761 caagcatcgg gtgcagtgaa agagttgatg cagaccagca aggctgctcg cacggagcta
     2821 gcgcgcttgg ttcgctccga gatccagaat cagatcgagg gcctcgggct ggctacgcag
     2881 cgagacctgg agcgactcga gcggagggtt gcgcgggtcg agggcgatcg taagaagacg
     2941 gcggctcgga agacgtcgtc ctccggatcg aagaccacgg ctcggaaaac ctctaaggcg
     3001 aagaagtcga cgactcggaa gaagccagat ccggcccgga agacctctac ctagtcataa
     3061 tcgggtcatg gagcgccccg gagcaaacgc ggatcctgcc gtcctggatg aatgggcgcg
     3121 caggcaactg gagacggccg cggtgtccga cgacgcgact cgactgaaga tccttgacga
     3181 gcttcacgaa gctttcgagt cgggccttgc cgaggactct gcgcctagat cttgagctgg
     3241 ttcgtcgcgg actgatcgga tctcgcgccg ccgcgcgccg ggccatcgaa gaaggccgag
     3301 taacggttcg cggtttccct gccgaacgtg cgtcctcgct ggtgaccgcg gacgacccca
     3361 ttgccctggt gggggacccc gcgccattcg tctcccgagg cggtgagaag ctggacgcgg
     3421 cattacaggt attcgacgta gtggtgcggg caaggagatg gctcgatgcg ggtgcgtcca
     3481 cgggcggatt caccgatcga ctcctacaag gtggagccga ggaggtggtt gcgatagacg
     3541 ttggatacgg gcagttcgcc tgggagcttc gcaacgaccc gagggtcact ttgatcgaac
     3601 gaaagaacgt gcgtaccttg gagcgctccg accttccgtt ctcccccgac ggcgtggtgg
     3661 cggatctgtc gttcatctct ttgaccctgg tgatcggtgc cctggccgaa gtggcggcga
     3721 atcacgcgga tcatctcctg ctcgttaagc cgcagttcga ggtaggccgc acagacgtgg
     3781 acaagggagt ggtgcgcgac ccggccctat ggcgagccgc tatagaaagg gtggtcgtct
     3841 ccgctcgaga gcacgacctt gaactgcggg acgtctcgct cgcgcatcca ccaggtcccg
     3901 ccggtaaccg cgagttcttc gttcacctaa cccgcggagg tcaaagcgga accgacgcga
     3961 tcgagcgaga ggtcgaaagg gctgccggtg agatttgagc gcgttgcgat agtcgcccac
     4021 gccagcaagt cgttggcgcg cgaggcggag accaccctca aagagtggtt ggccgaacgc
     4081 ggcatcgagt tgtcgggaga gcagccggac ctcgtgatct ctcttggggg tgatggaacg
     4141 atgttgcgcg cggcgaaggt cgctcacgcg gcggacgcgc ctctgctggg tgtgaacctg
     4201 ggtcgggtcg gctatctgac tgagatcgag atgtcgcagg agatggaatc gctcgagaag
     4261 gtcttcgccg gcgactacgc gatcgaagaa cggatgatgc tcgattgcag ggtcgagatc
     4321 gcttcggaga tccatccgtt cctcgggttg aacgaggtgc tcctcgagag atcgtctcgt
     4381 caccgactcg taaatctaga ggtctgggtc ggtgaagagc agctgggctc gttcagcgcg
     4441 gacggcgtga tcgtcgcgac gcctaccggc tcgaccgcat acgcgctcag tgcaggggga
     4501 cctatcgtgt ctccgagagc cgagtgtctc gtcgttgtcc ccgtcagtgc acacatgatc
     4561 ctcagcagac cgttcgtgtt cgcggccgac gagacggtga cgttgaaggt gggctcgggc
     4621 tcgagccagg gggcaacgct tgttgtagac ggcgcggccg gggtggaggt cccatccggg
     4681 aatgcagtcg acgtcgggcg gaacccgagg ccactgcgtc tcgtgaagct ggcgggtccc
     4741 gggttcatag agcgattgcg gatgaagctc ggcctgccgg ggtgagtgga gccccgcccc
     4801 ataggattca gacgtgctgg tagagctggt ggtggccggt ctcggcgcga tcgaccgtgc
     4861 cgaggtcaag ctcggacccg gttgcacggc cctgaccgga gaaacgggag cggggaagac
     4921 gctcctggtc gccgccgtcg gtttgcttgc gggcgcccga ggcgatcgag ggctgatacg
     4981 tgaagggcag gccgaggcga gcgtcgaagg gcgcttcatg gttcctcgca cccatccggc
     5041 ggcactcctg gttgccgagc tcggattcca agccgacgac gcggccgacc cggtggaggt
     5101 agtcctaacg cgttcgatcg caacgagtgg aagccgcgcg agggtcaacg gccggctggc
     5161 gacactgacc gttctcagcg aacttggagg tctcctaatc gagatagcga gccagcacga
     5221 gcaccagagg ctcgcgtcga cgaggtctca acgggcgctc ctcgacgcat tcggcggtcc
     5281 cgacgtgatc gaactcgccg gcaaagtgga ggctgcagtt cgagcagcgg tcgcggcggc
     5341 tcgtgaactc gaagagctcg aagcctcgcg gcgtgaacga gaacgcgagc tggatgtgct
     5401 gcgttacgag gttgctgaga tcgaaggggc cgcactgcag gaaggcgagt cggacgatct
     5461 gaagacggtt gccgctcggc tcgaacacgc cgaggagatc gcaggggcga gcagggacat
     5521 ctcccgtctc cttacgggcg agagcggtgt caccgattcg ctggtccagg ctcaacgcgt
     5581 ggcggagaaa gccgcatccc tggacggatc cttcgggacg gtggccgaac ggttgcaggc
     5641 cctggtcgtc gaggccgacg acgtggctca ggacgtggtg cgccgtgagg tcgcgcccga
     5701 cccagccgcc ctcgctgcga ctcgcgaacg gctcgaccag atcg
//
LOCUS       DATMED010000103         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1612416, whole genome shotgun sequence.
ACCESSION   DATMED010000103 DATMED010000000
VERSION     DATMED010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612416"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..927)
                     /locus_tag="VNP73_02595"
     CDS             complement(211..927)
                     /locus_tag="VNP73_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64838.1"
                     /translation="MTTKTRKLRPSPALVISCVALCFALVGTAVAAPKLAVRSAQIVN
                     GTVRTVDFRDNAVTSPKIADATVTATDLGTDSVGSEEIAKDAVNSDEIAKDAVKTDEI
                     ADNAVASPEVAPDSLTAGDLAANSVGSSEVADESLTQADLGVNSVNELEVAANAISAD
                     ELTAVTLRSNADTLAAGQNGAVAVSCQGNEQIISGGGASVFGVEMTSNAPSGNGWFYS
                     ASNKTAATRTITVYALCLAP"
     gene            1057..2430
                     /locus_tag="VNP73_02600"
     CDS             1057..2430
                     /locus_tag="VNP73_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWL64839.1"
                     /translation="MASAEKVCVIGAGSSGLTACQVLGARGIPFDCFEKGSMIGGNWR
                     YENDNGTSSAYRSLHINSARKLMSYKAFPMPEDYPDYPSHWQVAKYFDDYAERFGLKE
                     KITFGTAVVGVVPVPADQRGRGLNRYPDQSSTAPWEVTVEDADGERRTETYGAVIVAN
                     GHHWKPRWPEPPFPGADAYEGEQIHVHHYREPDVLEGKRVLVLGIGNSAVDVAVESSR
                     IAEKTFLAMRRGAYVLPKFLGGKPIDESAPPISTYLPMSVRRVFMSRLLKMTVGEMTD
                     YGLPKPDHKLLEAHPTVSSELLPRIGHGDITVKPTIERFAGGRTVRFDDGSEENVDLV
                     VYCTGYKIEFPFLDASVFAAQDNRMPLYKRAVSVENPGLYFLGFIQPLGPIMPIAEAQ
                     SEWIADLLTEGAALPPANEMRAEIAAYERWMDKRFVSSKRHTIEVDFYPYLREIRRER
                     RRTKQAA"
BASE COUNT          490 a          960 c          993 g          513 t
ORIGIN      
        1 cgaccgaacc cgctcccttc ggtcgctcac aagttccgct ggaggggcac cccggccctt
       61 ccacgcggaa cgcgaaacgg ccgggctcgg ctcactccat cctcgccgag catgatggac
      121 agttgtgccc cctatagggg gtaaaagggt ccatgaaacg cgaacggccg ggcgtttgcc
      181 cggccgtttc gcgcttggtt ggttgggtgt ctacggggcg aggcagaggg cgtagacggt
      241 gatcgtgcgg gttgcggcgg ttttgtttga cgctgagtag aaccagccgt tgccgctggg
      301 ggcgttggag gtcatctcga cgccgaagac gctggcacca ccgccgctga tgatctgctc
      361 gttgccctga cagctgaccg cgacggcgcc attctggccc gcggcgaggg tgtcggcgtt
      421 gctgcgcagc gtcaccgcgg tgagctcgtc ggcactgatc gcgttggcgg cgacctcgag
      481 ctcattgacc gagttgacgc ccaggtcagc ctgggtcagc gactcgtcgg ccacctcgct
      541 gctgccgacc gagttggcag ccaggtcacc ggcggtcagt gagtccgggg caacctctgg
      601 ggaggcgacc gcgttgtcgg cgatctcatc cgttttgacc gcgtctttcg cgatctcgtc
      661 ggagttgacc gcgtctttcg cgatctcctc gctgccgacc gagtcggtgc cgagatcggt
      721 ggccgtcacg gtggcatcag cgattttggg cgaggtgacc gcgttgtcgc ggaagtcgac
      781 cgtgcggacc gtgccgttga cgatctgagc tgaacggacg gcgagcttcg gtgccgcgac
      841 ggcggtgccg acgagggcga agcacagcgc tacgcagctg atcacgaggg cgggggaggg
      901 gcggagcttg cgggtcttgg tggtcatcgg gggactcctt tcgtttggtt ttgattcccg
      961 atggccacag cttcggccgg atccggctct gctactacgg accccagggg tgaagttcct
     1021 cacccctgtg cgtgcgggtt gggtagattt ggccgcgtgg ccagtgcgga gaaggtgtgc
     1081 gtcattggag ccggcagctc cgggctgacg gcttgccagg ttctaggcgc ccgtgggatc
     1141 cctttcgact gcttcgagaa ggggtcgatg atcggcggga actggcgcta tgagaacgac
     1201 aatgggactt cgtccgcgta ccgctcgttg cacatcaaca gcgcgcggaa gctcatgtcc
     1261 tacaaggcgt tcccgatgcc ggaggactac ccggactacc caagccattg gcaggtggcc
     1321 aagtacttcg acgactacgc cgagcgcttc ggcctaaaag agaagatcac ttttggcacc
     1381 gcggtcgtcg gcgtcgtccc agtgcctgct gaccagcgcg gtcgaggttt gaaccgctat
     1441 ccggaccaaa gctcgaccgc gccttgggag gtgaccgtcg aggacgcaga cggcgagcgg
     1501 cggaccgaga cctacggggc cgtgatcgtc gccaatgggc accactggaa gccgcgctgg
     1561 cccgaaccac catttcccgg cgccgacgct tatgagggcg agcagatcca cgtccaccac
     1621 taccgggagc ccgacgtgct cgagggcaag cgggtgctgg tgctggggat cgggaactcc
     1681 gccgtcgacg tcgcggtcga gtcgagccga atcgccgaga agaccttcct cgcgatgcgg
     1741 cgcggcgcct acgtcttgcc gaagttcctc ggcggcaagc cgatcgacga atcggcgccc
     1801 ccgatcagca cctacctgcc gatgtcggtg cggcgggtct tcatgagccg gctgttgaag
     1861 atgacggtcg gcgaaatgac cgattacggc ttgcccaagc cagaccacaa gctgctcgag
     1921 gctcacccga ctgtctcctc ggagctcttg cctcgaatcg gccatggcga catcacggtc
     1981 aagccgacca tcgagcgctt cgccggcggc cgcacggtcc gcttcgacga cggcagcgaa
     2041 gagaacgtcg acctagtcgt ctactgcacg ggctacaaga tcgagttccc cttcctcgat
     2101 gcgagcgtct tcgccgccca ggacaaccgc atgccgctct acaaacgcgc cgtctccgtc
     2161 gagaacccgg gtctctactt cctcggcttc atccagcccc tcggtccgat catgcccata
     2221 gccgaggccc agtccgaatg gatcgccgac ctcctcaccg agggagcggc gcttccccca
     2281 gctaacgaga tgcgcgccga gatcgccgcc tacgaacgct ggatggacaa gcgtttcgta
     2341 tcgtcgaagc gccacacgat cgaggtcgac ttctacccgt accttcgcga gattcgtcgc
     2401 gagcgcagga gaactaaaca ggcggcttaa cgggaggggg cggggttccc ctccgccgga
     2461 acccgcgtcg gcgggctggc gatggggtca gcgcgtagcg gctcacaagt tcctgcggag
     2521 gggaaccccg ccccctcccg tggaggggca ttccgtccct cccgatcgca cctccgtgag
     2581 ggccgcggcg tggcccgaga ggcagaaccg gctgcggtcg ccagcggttg aagccgttgc
     2641 ccgagtcgtc cccggtggtt tccggctcgg gttctctact agcccaccag acgagccata
     2701 gcgcgccgaa gaccgggatc ttcagcacga ccagcaggaa gaagattggc cagaatccct
     2761 ccatcgggcc agtgtagaca cccggcccgc gccagcgggc tcaaaggccg cgagcccagg
     2821 ccgagagcgc ctcgaactgg acctcctcga ggccgaggtg aggctcggtg gggacgagca
     2881 gggtcggctc ctcacgcccc cgggcccact cgtagcagct gtcgtcgaag ctgtcgtcga
     2941 tccaggccag cggccg
//
LOCUS       DATMED010000104         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3913320, whole genome shotgun sequence.
ACCESSION   DATMED010000104 DATMED010000000
VERSION     DATMED010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3913320"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VNP73_02605"
     CDS             <1..722
                     /locus_tag="VNP73_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HWL64840.1"
                     /translation="ELEEALRQTASLLSTLTDHAAVVFSPALDRSTIRHLELVSLPQN
                     RAMVVVVTDAGRVYNQVVGHDGDLNEDEISRIGRMLNAVVAEVAVEDVGETITKSLER
                     FRPEERTLVSMLSDVLKSEFARRDAEKMFLDGTANIIDEHKFADLETVRHVIEALEHR
                     RLLLELLADALGAGSVSVRIGAENPEEMQFCSVIAAPYGIAGHPLGSLGVVGPTRMDY
                     RRTIAAVHEVAANLGRMLNGI"
     gene            738..1829
                     /gene="dnaJ"
                     /locus_tag="VNP73_02610"
     CDS             738..1829
                     /gene="dnaJ"
                     /locus_tag="VNP73_02610"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWL64841.1"
                     /translation="MARDHYEVLGVSRQATQEEIKKAYRALARRYHPDANQTDPAAEE
                     LFKEVTVAYETLSDPQKRQNYDTFGDSRLGGSGFGDFGGISDLFSSFFGGGFSSASRT
                     GPARGADVLAEVALTLEEAAEGVEKDVEITTLVACDVCDGSGSAPGTFPSRCSECGGT
                     GEQRHVRRTVFGNVMTASTCSRCGGRGEEIVDPCKGCHGSGRTQVTEMMTVRVPPGVD
                     DGAQLRVTGRGQAGVRGGRSGDLYVGIRVMPHEIFQRAGDDLACQVTIPMTVAALGGT
                     VEVPTLEGPDAIDVRPGTDSGEIIHLRGRGMPRLTGSGRGELVVLLKVETPTDLTPEQ
                     DEMLRTFAESRNETVGERGLFQKIKEAFS"
     gene            1839..2570
                     /locus_tag="VNP73_02615"
     CDS             1839..2570
                     /locus_tag="VNP73_02615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWL64842.1"
                     /translation="MSGPWFIAPSSGWSGTEIRLPPEEAHHARVLRVGTPDVITVTDG
                     KGSVARCAVRVAEPTEVVAEILEQEERRRSTPEIVVYQGAAKGHKNDEVIERLAELAV
                     AEVSVFSSERSVVRWDRAKSEKLAERWGAIARSAAKQSRNPYVCRTSAGLSFTELQRR
                     VAKEPLALVLWEEAMLPLRTALVDTTERLALVVGPEGGLSQAEAESLADAGGQLVSLG
                     PHILRTEVAPVVAVSALMYRYGLIG"
     gene            2612..>3303
                     /locus_tag="VNP73_02620"
     CDS             2612..>3303
                     /locus_tag="VNP73_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HWL64843.1"
                     /translation="MPSVSFTTLGCRLNQAESDLMAEDLAAAGVVQASSGADPDIVVI
                     NTCTVTREATKASRMAIRRAVRNHPEAKVVVAGCYAVSNRDEIEAIEGVDAILSNNDK
                     ERFTEVLGRDPGTRPLLQLGMRPQRDHTGAPARVRANLKAQTGCDEFCTFCIIPMTRG
                     PLRSMDEDALIEEASARVRGGARELVLTGVHLGKYRVDQGGDERDLIRLLERLLDIPG
                     VLRLRLSSILSQ"
BASE COUNT          604 a         1009 c         1101 g          589 t
ORIGIN      
        1 gggagctcga agaggcattg cgccagacgg cctccttgct gtcgacgctt accgatcacg
       61 ctgcggtggt gttcagccca gcactggatc gcagcacgat caggcacctc gagctcgtct
      121 cgttgccgca gaacagggcg atggtcgtgg tcgtgaccga tgcgggccgt gtgtacaacc
      181 aagtcgtcgg gcatgacggg gacctgaacg aagacgagat ctctcgtata ggacggatgc
      241 tcaatgcagt cgtcgccgaa gtggcggtcg aggacgttgg cgagaccatc accaaatcgc
      301 tcgaacgttt ccgaccggag gaacgaacgc ttgtctccat gctctccgac gtcctcaaga
      361 gcgagttcgc tcgccgcgac gccgagaaga tgttcctgga cggaacggcc aacatcatcg
      421 acgagcacaa gttcgccgat ctcgagaccg ttcggcacgt gatcgaagct ctcgagcatc
      481 gtcgcctact gctcgagctc cttgccgacg ccctcggcgc gggcagcgtt tcggttcgca
      541 tcggtgccga gaatccagaa gagatgcagt tctgctcggt gatcgccgcg ccgtacggga
      601 tagccgggca ccctctcgga tctctcggtg tcgtcggccc gacccggatg gactacaggc
      661 gaaccatcgc ggccgtccac gaagtggctg cgaacctcgg ccggatgctt aacggcatct
      721 gatctaggag aaacatcgtg gctcgagatc actacgaggt gttgggtgta tcacgccagg
      781 cgacgcagga agagatcaag aaggcgtacc gcgccctcgc tcgtcgctac catcccgacg
      841 ccaaccaaac cgatcccgcg gcagaagagc tattcaaaga agtaacggtt gcatacgaga
      901 cgctttccga tccgcagaaa cgccagaact acgacacgtt cggcgattcg cgtcttggcg
      961 gaagtggttt cggtgatttc ggagggatat cggacctgtt ctcatcgttc ttcggcggag
     1021 gattctcgtc ggcttcgcga accggtccgg cgcgcggcgc agacgttctg gccgaggtgg
     1081 ccctgaccct ggaagaagcc gccgaggggg tcgagaagga cgtcgagatc acgactctcg
     1141 tcgcgtgcga cgtatgtgac ggcagcgggt cggcccccgg aaccttcccc agccgctgtt
     1201 ccgaatgtgg cggcacgggc gaacaacgcc acgtgagacg cacggtgttc ggcaatgtga
     1261 tgacggcgtc gacgtgttcg cgctgcgggg gacgcgggga agagatcgtg gatccgtgca
     1321 agggatgcca cgggagcgga cgcacgcagg tcaccgagat gatgaccgtg cgagtgccgc
     1381 ccggcgtcga cgacggtgct cagttgcgcg tgacgggtcg cggccaagcc ggtgtccgcg
     1441 ggggccgttc gggcgacctc tacgtcggta tccgcgtgat gccgcacgag atcttccaga
     1501 gagcggggga cgatcttgcg tgccaggtaa cgatcccgat gaccgtcgca gccctgggag
     1561 gcacggtaga ggtccccacc ctcgagggtc ccgacgcgat cgatgtgcga cccggtaccg
     1621 attccggcga gatcatccac ctgaggggac ggggcatgcc tcgtcttacc ggtagcggtc
     1681 gcggcgagct cgtcgttctc ctcaaggtcg aaacacctac cgatctcacc cccgagcagg
     1741 atgagatgtt gcggacgttc gccgagtccc ggaacgagac ggtcggcgag cggggcctgt
     1801 tccagaagat caaagaagcc ttctcctaag gaggcacctt gtcgggccca tggttcatcg
     1861 caccctcgtc cggctggagc ggaacggaga tacgcctccc cccggaagag gcgcatcacg
     1921 ctcgcgtgct gcgggtcggc actccagacg tcatcacggt gaccgatggg aaaggctccg
     1981 ttgcgcgctg cgcggttcgg gttgcggaac cgaccgaggt cgttgccgag atcctcgagc
     2041 aggaagaacg acgcaggtcg acgcccgaga tagtcgtcta ccagggtgcc gccaagggac
     2101 acaagaacga cgaagtgatc gaacgtctgg cggagctcgc ggtggccgag gtgtccgtgt
     2161 tctcctccga acgttcggtg gtgcgctggg atcgggcgaa gtcggagaag ctggccgaac
     2221 ggtggggcgc gatcgctcgc tcggcggcca agcagtccag gaatccatac gtgtgccgga
     2281 cgagcgcggg cctgtcgttc accgagctgc agcgccgcgt tgccaaggaa ccactagcac
     2341 tcgtcctgtg ggaagaagcg atgttgccgc tgcgaaccgc actggtggat acgaccgagc
     2401 ggctggcatt ggtggtcggt cccgagggtg ggttgtccca agctgaagcg gagtctctcg
     2461 ccgatgccgg agggcagctc gtatcgctcg gcccccacat ccttcgcacg gaggtcgctc
     2521 ccgtggtggc tgtgtcggct ttgatgtacc gctacggcct catcggctag ctcgcgcggt
     2581 ggcctcggcc agcgcgcagt accgttcgtt gatgccttcc gtttccttca ccaccctcgg
     2641 atgcaggctc aatcaggccg agtccgacct catggccgaa gatctggccg cggccggcgt
     2701 tgtgcaggcc tcttccgggg ccgaccccga catcgtcgtc atcaacacct gcacggtcac
     2761 gcgcgaagca acgaaggcgt cccggatggc gatccgccgt gccgttcgga accatcccga
     2821 agcgaaggtc gtggtggccg gttgctatgc cgtctcgaat cgcgatgaga tcgaagcgat
     2881 cgagggtgtc gacgcgatcc tttcgaacaa cgacaaggag cggttcaccg aggtcttggg
     2941 ccgcgatccc ggcacgcgac ctctgcttca gttggggatg agaccgcagc gcgatcacac
     3001 gggtgctccg gcgcgggtcc gtgccaatct caaagctcag accggatgcg acgaattctg
     3061 caccttttgc atcatcccca tgacccgggg tccgcttcgc tcgatggacg aagacgcttt
     3121 gatcgaggaa gcctcggcgc gggttcgggg gggagcacgt gagctggtgc tgaccggggt
     3181 tcacctcggc aaatatcgag tcgaccaagg tggcgatgag cgggatctga tccgcttact
     3241 cgaacgattg ttggacatcc ccggcgtgct acgccttcgt ctgtcgtcga tcctgtcgca
     3301 gca
//
LOCUS       DATMED010000105         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3527103, whole genome shotgun sequence.
ACCESSION   DATMED010000105 DATMED010000000
VERSION     DATMED010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527103"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..415)
                     /locus_tag="VNP73_02625"
     CDS             complement(167..415)
                     /locus_tag="VNP73_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64844.1"
                     /translation="MNGGGIPNWLALGVALLLIAGGLLWLRSRPDEDPCGRWQRLVVH
                     EAELLSSQRKRPPEAFYAQAARTFSESRPVGCDSPSPS"
     gene            458..1267
                     /locus_tag="VNP73_02630"
     CDS             458..1267
                     /locus_tag="VNP73_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWL64845.1"
                     /translation="MPIADLGDINLHYSEHGDGDSVLGVMGFALDQRFWSAQIPAVTA
                     THRFITFDNRGLGRSTGGSVQTIDEMADDSIRLLDHLGIERTVVFGVSMGGAIAQRIA
                     IDHPDRVSALILGVTWARPIEFMRRQSLLARDIIGQRGAEGLVEAALLWMFTPKFFEV
                     GKETIDQLVTAFFAESESDATSPEVLLAQLEAIEKHDCLADLGRVGVPTLVLGGKADM
                     MVPVFAAEEIAQAIPGSRLRLFETGHGCMIEEMQEFNREVSDFLGSLRAAG"
     gene            1353..1685
                     /locus_tag="VNP73_02635"
     CDS             1353..1685
                     /locus_tag="VNP73_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64846.1"
                     /translation="MRRTLAALILALLVLGLGAAPALAQRDPFEPAINEEGEVQPEDE
                     DEDEEEPAEDEDEDEDEGTAGDELADTGASVEPMLVLAYALLAFGIAAVIVARLYSSS
                     HRTRPRRR"
     gene            1734..1958
                     /locus_tag="VNP73_02640"
     CDS             1734..1958
                     /locus_tag="VNP73_02640"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HWL64847.1"
                     /translation="MTLCSKPRCARPGSVALAYDYATRRAVLDDPPGGQMPPHLYALC
                     DACAGVLSPPRGWTLEDHRGRILDLAQPSF"
     gene            2015..2296
                     /locus_tag="VNP73_02645"
     CDS             2015..2296
                     /locus_tag="VNP73_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64848.1"
                     /translation="MAGTLYSELRKLDRHELRRVLIYVKGLLNALDDDAGAPEPGDEK
                     VTYRLESVRCGKPNCKSCPHGPYWYAYYRDGDRLKSRYIGKQLPDDDRS"
     gene            complement(2590..3267)
                     /locus_tag="VNP73_02650"
     CDS             complement(2590..3267)
                     /locus_tag="VNP73_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64849.1"
                     /translation="MPDLRPPNIRFPSRLINRLRARGPGEVLALATARLRETFASSDT
                     LIFFVRDCGGTFDAPDDLLFRRATADDAGIYARDIGTDSTSTFRRRLSDATRCFVVES
                     KGHLVHASWVTTAAAWTRELHSYVKPPRGDAYIYESFTRADTRGRGVYPLALNGICSR
                     LGQDGVAHAWVAAEEDNPASIRAITKASFEEAFRVSYERRFGGLHIGEFSGEMAESGR
                     TFLHRHP"
     gene            complement(3292..3558)
                     /locus_tag="VNP73_02655"
     CDS             complement(3292..3558)
                     /locus_tag="VNP73_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWL64850.1"
                     /translation="MELITTPQAEWRERAACLPFPAVLFFGLDDNESPIERRAREEEA
                     KSICSTCAVQGQCLEFAISAKEPYGIWGGLTEVERKGSALVRSN"
     gene            3650..4990
                     /locus_tag="VNP73_02660"
     CDS             3650..4990
                     /locus_tag="VNP73_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWL64851.1"
                     /translation="MPADLTPIFKAYDIRGIFPDALDEDAAARIGAAFVEWLARDQIV
                     MGRDMRTSSPGLADAFAAGASAAGARVVDVGLVSTDALYFASGRLDLPGAMFTASHNP
                     AQYNGIKLCREKAAPIGQESGLLDILALATKATVTPQEARVEQRDVLAEYADHCRSLV
                     DRSHLGRLKVAIDAGNGMAGKTVPEVFGPLPFEVVPLFFELDGTFPNHPANPIEAENL
                     VDLQRAVEREGCDLGIAFDGDADRIFLVDDKMRLVTGSTTTAMVAERLLKKNPGETII
                     YNLICSWTVPEVVKENGGTPVRSRVGHSFIKQVMAESGAIFGGEHSGHYYFRDNFRAD
                     SGMIAALLVLEAICEAGRPLSELLEPYDRYFDSGEINSEVGDQQAVLEKLSEVYKDGQ
                     QDRIDGLTVEYEDWWFNCRPSNTEPLLRLNLEARTKELMDSKRDEALNVIREGS"
     gene            4990..>5360
                     /locus_tag="VNP73_02665"
     CDS             4990..>5360
                     /locus_tag="VNP73_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64852.1"
                     /translation="MRKLVVAVACLVFVVLPLTAGAVGSDDPNDTTGRLDVGYLGTYV
                     QGSERVFEITMYEQWRNRLFRFRKEHAQRGSIVFLFNTDADKAPELSVTPERRDGRWV
                     GRLCRVQTGRCERARVWRESD"
BASE COUNT         1027 a         1666 c         1688 g          979 t
ORIGIN      
        1 gatcccaaga tgaaccaccg aaaagggatg cgggcctcgt cgggaatgac tcgccaaccg
       61 tagacattta tcgctatgac cgagattgca gcaagtatca gaggggcagt cgacatttga
      121 agttctagcc ccttaccttt cagtccccct tcgtcgccgc ggcctattag ctaggcgaag
      181 gggaatcgca gcccacggga cgactctcgg agaacgttcg tgcggcctgc gcgtagaaag
      241 cttccggagg gcgcttcctc tgagaggaca gtagctccgc ctcgtggacg accaacctct
      301 gccagcgtcc acacggatcc tcgtcgggcc gggacctcag ccacagcaag ccacccgcga
      361 tcaacagcag cgcgacgcct agcgcaagcc agttcgggat ccctccgcca ttcacgtaga
      421 gaaggtaatc acaatccggc tgataccttt cgtttccatg ccgatcgcag acttaggtga
      481 catcaacctt cactattccg aacacgggga tggagattca gttctcggcg taatgggctt
      541 cgcacttgac cagcgcttct ggtcggccca gatcccggcc gtgacggcga cccaccgctt
      601 catcaccttc gacaacaggg gactcggccg ttccaccggc ggctccgtcc agacgatcga
      661 cgagatggcc gacgactcga tccggctact ggatcatctc ggcatcgaac gaaccgttgt
      721 attcggggtc tcgatggggg gagctatcgc ccaacgaatc gcgatcgatc accccgaccg
      781 agtctcggcc ctcatcctcg gtgtgacctg ggctcgcccg atcgagttca tgcgacgcca
      841 gagtctgctc gcgcgcgaca tcatcggcca gcggggagcc gaggggctcg tcgaggcggc
      901 actgttgtgg atgttcacgc cgaagttctt cgaggtcgga aaggaaacga tcgaccaact
      961 agtgacggcc ttcttcgccg aatccgaatc ggatgcgacc agccccgagg tgttgctggc
     1021 ccaactagaa gcgatcgaaa agcacgactg cctggcggac ctgggccgcg ttggggttcc
     1081 gacgctggtg cttgggggga aggccgacat gatggtgccg gtcttcgcgg ccgaggagat
     1141 agcccaggcg atcccgggga gccggctgcg cctcttcgag accggccacg gctgcatgat
     1201 cgaggagatg caggaattca accgagaggt gtccgacttc ctcggctccc tgcgagcggc
     1261 cggttaggta acgagtcgta cccaagaaac ttttgtaact ttcttcgcgc tctcaagtgg
     1321 gttatccggg cgtcgacacc taagatgaac ccatgcgcag aaccctggcc gccctgatcc
     1381 tggccctcct ggttctcgga ctgggagccg ctcccgctct agcccagcgt gatcccttcg
     1441 agccggccat caacgaggag ggagaggtcc agcccgaaga cgaggacgag gacgaagagg
     1501 agcccgccga ggacgaagac gaggatgagg acgaaggaac ggctggggac gagttggcgg
     1561 acacgggggc cagcgtggaa cccatgctgg tgcttgccta cgccctgctg gcattcggca
     1621 tcgccgcggt gatcgtagcc cgcctctact cgagctccca ccgcactcgc cctcgacgac
     1681 gctaggtcgc gaatccgcct ctccgagagg ctcttgccgt accctcctcg accgtgaccc
     1741 tgtgctcgaa gccccgctgc gctcgccccg gttcggtggc gctggcgtac gactatgcga
     1801 cccgccgggc cgtgctcgac gatccccctg gcgggcagat gccgccccac ctctatgcac
     1861 tgtgtgacgc atgcgccgga gtcttgagtc cacctcgagg ttggacgctc gaagatcacc
     1921 gcggtcggat cctcgacctt gcgcaacctt ccttctaaac ttcttaggta agtcctgtac
     1981 ctaagtccct ggtggcccgg gagcggagtc gaacatggcc ggcaccctgt actcggagct
     2041 tcggaagctc gatcggcacg aacttcgtcg cgtcctgatc tacgtcaagg gtttgttgaa
     2101 tgcgctcgac gacgacgcgg gagctccgga accgggcgac gagaaggtca cgtatcgctt
     2161 ggaatccgtc cggtgtggga agccgaactg caagtcgtgt ccgcacggtc cctattggta
     2221 cgcgtactac cgcgacggcg accgcctcaa gtcgcgctac atcggcaagc aacttccgga
     2281 cgacgatcgg agctagggat cagtccgtcg aacgccgaac gtctcgacgt tcggcggaaa
     2341 ctaataagaa atgcgggcgc tcggagcccg aaaaccgtcc ggcaacaggt gccagttgag
     2401 ggggtcgtgc cggtaagcag ccccgttgac gagagaagct ctcgaccgcg gggcgcatgc
     2461 cggatcctgg ctccgaacgc ccgcagaacc cgtctgaaga cgtgtgcttg ctttactccc
     2521 tcacgagcat tattgcgctc ttacaagcgc ttgactatcc ccgccaccgc tttatttgcc
     2581 gagtgatcct cacgggtgtc gatgtaggaa cgttcgtccg gattcggcca tctcgcccga
     2641 gaactcaccg atatgtaacc cgccgaaacg acgttcgtag gagacccgga aggcctcttc
     2701 gaaggaagcc ttggtgatcg ctcgaatgga agcggggttg tcctcctccg ctgctaccca
     2761 cgcatgcgcg acaccgtcct gccccagcct cgagcagatc ccattgagcg caagggggta
     2821 aacgccgcgg ccgcgcgtgt cggcccgcgt gaaggattcg tagatatagg catcgccgcg
     2881 tggaggtttc acgtacgagt gcaactctct cgtccaggcc gccgcggtag ttacccaact
     2941 tgcgtgaacg agatgacctt tcgattccac gacgaagcag cgcgtcgcgt ccgaaagacg
     3001 tcttcggaaa gtcgaggtgg agtcggtccc gatgtcgcgt gcgtagatcc ctgcgtcatc
     3061 ggccgtcgcc cggcggaaca agaggtcgtc gggcgcatcg aaggtgccgc cgcagtctcg
     3121 gacgaagaag atcagtgtgt cggacgaagc gaacgtttca cggagccgcg ccgtcgccaa
     3181 tgccaggacc tcgcccggcc cccgcgctcg gagccggttg atgagacgcg acgggaaacg
     3241 gatgttaggt gggcgtaggt ccggcacaag ccgacttcgg ttgcgagttg ctcagttcga
     3301 tcggaccagc gcggaaccct tgcgctcgac ctcggtcagg cctccccaga tcccataagg
     3361 ctctttagcc gagatcgcga actcgaggca ctgcccctgt accgcgcacg tcgaacagat
     3421 cgatttggct tcctcttccc gggcgcgacg ttcgatcgga gactcgttgt cgtcgagtcc
     3481 gaagaacagc accgcaggaa aaggcaagca cgccgcccgc tcccgccact ccgcctgtgg
     3541 ggtggtgatc aactccatgg gcgggaatgg ttgcagttga agcccgcttc gtcaaaaggg
     3601 ctccggatcc gcatgggccg ggggccgaga ccccatctag gatgcgcccg tgccggcgga
     3661 cctgactccc atattcaagg cctacgacat ccgaggcata ttcccggatg cgctggacga
     3721 ggacgccgcc gcccggatcg gtgcggcctt cgtggaatgg cttgctaggg accagatcgt
     3781 gatgggtcgg gacatgagga cgtcgtctcc tgggctcgcc gacgccttcg cggcaggagc
     3841 gtcggctgcg ggagcgaggg tcgtcgacgt gggcctcgtc tccaccgatg ctctctactt
     3901 cgcttccggg cgtctggacc tgcccggagc gatgttcacc gcctcccaca accccgcgca
     3961 atacaacggg atcaagttgt gccgggagaa ggcggctccg atcgggcaag agagcgggtt
     4021 gctcgacatc cttgccctcg ccaccaaggc cacggtgacc ccacaggagg ctcgcgtcga
     4081 gcaacgcgat gtcctggctg aatacgccga ccactgccgg tcgctggtcg atcgctcgca
     4141 cctcggaagg ctcaaggtcg cgatcgatgc agggaacggg atggccggca agacggtccc
     4201 cgaggtgttc ggcccccttc cgttcgaagt cgttccgttg ttcttcgagc tcgacgggac
     4261 gtttcccaac caccctgcca acccgatcga agccgagaac ctggtggatc tacagcgcgc
     4321 cgtcgagcgg gaagggtgcg atctcggcat cgcgttcgac ggtgatgcgg atcgcatctt
     4381 cctggtcgac gacaagatgc gattggtcac cggttcgacg acgaccgcga tggtcgcgga
     4441 gcggctgctg aagaagaatc ccggcgagac gatcatctac aacctgatct gctcgtggac
     4501 ggtccccgag gtagtgaagg agaacggtgg aaccccggtc cgctcgcggg tcgggcactc
     4561 gttcatcaag caggtgatgg cggagagcgg cgctatcttc ggcggagagc actccggcca
     4621 ctactacttc agggacaact tccgtgccga ttccgggatg atcgcggccc tgctcgtgct
     4681 cgaagcgatc tgcgaggcgg gccgacccct gtccgaatta ctcgagcctt acgaccggta
     4741 tttcgattcg ggtgagatca attcggaggt gggcgaccag caggccgtac tcgagaagtt
     4801 gtcggaggtc tacaaggacg gccagcagga tcggatcgat ggccttacgg tcgagtatga
     4861 ggattggtgg ttcaactgca ggccttcgaa caccgagcct ttattgcgcc tcaatctaga
     4921 ggcgcgaacg aaggagttga tggactcgaa gcgagatgaa gctctgaacg tcataaggga
     4981 gggatcttga tgaggaagct tgttgtcgcc gttgcgtgct tggtcttcgt ggtgctgcca
     5041 ctcaccgccg gcgcagtcgg atccgacgat cccaatgaca ccaccggccg gctggatgtc
     5101 ggttacctcg gcacttacgt acagggctcc gagcgcgtgt tcgagatcac aatgtatgag
     5161 cagtggcgca accgtctctt ccgtttcagg aaagagcacg cgcagcgagg ctccatcgtc
     5221 tttctgttca acaccgacgc cgacaaggca cccgaattga gcgttacccc cgaacggagg
     5281 gacggtcgat gggtgggacg gctctgtcgg gtccagaccg gcaggtgcga acgggccagg
     5341 gtgtggagag agagcgacca
//
LOCUS       DATMED010000106         3285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3152329, whole genome shotgun sequence.
ACCESSION   DATMED010000106 DATMED010000000
VERSION     DATMED010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3152329"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VNP73_02670"
     CDS             <1..1200
                     /locus_tag="VNP73_02670"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL64853.1"
                     /translation="VVFNGMSDNGDPVLVASKNEGASWDVVFDAHGFTADPYMYVDPD
                     TSRIFANDLIPPCHLISYSDDEGEAWTTTPPQGCGVNEDHQTIFSGPAPKGGEEPSGY
                     KNVVYLCAIGMGISVGSAGSICSKSLDGGNSFIPTGTPAFTDDPRQSGDYGIPSRCNG
                     ANAHGSVGPTGTVYLPRGWCGQPWLAISKDEGATWERVQVSDLGMPCCADVDGLPGGL
                     YTHEAGVVEDAQGNIYYAWVAADRLPYLTVSRDGGKTWGKPLMIGPPGLREGLLPGIT
                     VGADGKIAVFYMGSTNSPWNPTKKEAEGSYENERWNAYVTMSVNADDKNPVFYSATLN
                     DPKHPMWIGACGPDPIRCGWGDFLDIVVSPKGEAWAVAVDLCTGADCSGSGEAVIGRL
                     VGGPSLR"
     gene            1197..2351
                     /locus_tag="VNP73_02675"
     CDS             1197..2351
                     /locus_tag="VNP73_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64854.1"
                     /translation="MKRAASGIAVALVVAAAIGPPSASAGPGQYGVTSDNVEHIAFIP
                     FEVGTATGASVVGKYLYVTSWKSFSIYDISNPLEPEQVTQVPFGFKFENENVSTNGKI
                     MLFAEQAPVDSLHVWDIEDKANPVEIATLDGAGTHTATCILDCKWSYGSYDLAGPEGA
                     STGAFLTDLSDPAAPKGVGHWNEKLPADKIHDVVEVAPGRVLTASAPLMFVDARKDPL
                     RPTLLAKGTNIDHRLHSVEWPRGGKDRFIMSSFETNATPRCEAGVGEFATWDASKWQK
                     THTFTVIDEFHITNGTYTDGRPAANGTGLGCSPHWFQAHSDFHDGGVVAAGFYDHGTR
                     FLEISSAGKIEEIGYFLPWGGATSAAYWVTDEVVYSIDYERGFDVLRFKP"
     gene            complement(2341..2805)
                     /locus_tag="VNP73_02680"
     CDS             complement(2341..2805)
                     /locus_tag="VNP73_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64855.1"
                     /translation="MQDGLAALKILFRVQIIAGFLILFILYKIDPGIGRIDVPWLMVV
                     LGAGVVGIAGAHWSTRRPLALESNESLASSYRTAFFSGFGLSEMPLMLAFVACFITKD
                     LWPYLSELPLWLIGMSLIAPTNSNLAKRQQEIAAQGSILDLAEALRAQNAKA"
     gene            2865..>3285
                     /locus_tag="VNP73_02685"
     CDS             2865..>3285
                     /locus_tag="VNP73_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64856.1"
                     /translation="MYRRLTLLGTLAALGMLVTAVPPAIGQETCEPWERRTIAEGLDR
                     IENLIPDGKGGMLISAGPRNAIERVTPDGEVTTAYEDVPGPGGLRIIGHTLYAVTNGN
                     IVDGVTDSPNGKIETFDLKSGERNIYSEGLTAPNGLVI"
BASE COUNT          647 a         1027 c         1053 g          558 t
ORIGIN      
        1 gtcgttttca acgggatgtc tgacaacggc gatccggtcc tcgtcgcatc caagaatgag
       61 ggcgcgtcgt gggacgtggt cttcgacgcc catgggttca cggccgaccc ctacatgtac
      121 gtcgatcccg atacgtcgcg catcttcgcg aacgacctga tcccgccttg tcacctcatc
      181 tcctacagcg acgacgaagg tgaggcgtgg accacgactc cgccccaagg atgtggcgtg
      241 aacgaggacc accagacgat cttctctggg ccggctccca aaggtggcga ggagccgagt
      301 ggctacaaga acgtggtcta cctgtgcgcg atcggcatgg gcatcagtgt gggttcggcc
      361 ggatccatct gctcgaagtc gctggacggc ggcaactcgt tcatcccgac cggcacaccg
      421 gcgttcaccg acgaccctcg tcagtcgggc gactacggga tcccgagtcg atgcaacggc
      481 gcgaacgcgc acggctccgt cggcccgacc ggcaccgtgt acctcccgcg gggttggtgc
      541 ggccaaccct ggctcgcgat cagcaaggac gagggtgcaa cttgggagcg cgtacaggtc
      601 tccgacctcg gcatgccgtg ttgcgcggac gtcgacgggt tgccgggagg gctctacaca
      661 cacgaagccg gagtcgtcga agacgcccag ggcaacatct attacgcgtg ggtggcggcg
      721 gatcgactcc cgtacctcac ggtgtcgcgc gacggcggca agacgtgggg caagccactg
      781 atgatcggcc ccccgggatt gcgcgaaggc ctgctgccgg gcataaccgt aggtgccgac
      841 gggaagatcg ccgtcttcta catgggcagc acgaactcgc cgtggaaccc gaccaagaag
      901 gaagccgagg ggagctacga gaacgaacgc tggaacgcgt acgtcacgat gagtgtcaac
      961 gcggacgaca agaacccggt cttctactcg gcgacgctca acgacccgaa gcacccgatg
     1021 tggatcggtg cttgcggacc cgatccgatc cggtgtggat ggggagactt cctggacatc
     1081 gtggtcagcc ccaagggtga agcgtgggca gtggccgtcg atctgtgcac cggagccgac
     1141 tgcagcggca gcggtgaagc cgtcatcggg cgattggtcg gcggcccgtc actccgatga
     1201 aaagagcagc gagtggcatc gccgtcgctc tggtggtggc tgcagccatc ggtccgccca
     1261 gcgccagcgc agggcccggc cagtatgggg tcacgtccga caacgtcgag cacatcgcgt
     1321 tcatcccgtt cgaggttggc acggctaccg gggcgagcgt cgtcgggaag tatctgtacg
     1381 tcacgagttg gaagagcttc tcgatctacg acatctcgaa cccgttggag ccggagcagg
     1441 tcacgcaggt gccgttcggt ttcaagttcg agaacgagaa cgtatccacc aacggcaaga
     1501 tcatgctgtt tgccgaacag gccccggtcg actcgctcca tgtctgggac atcgaggaca
     1561 aggccaaccc ggtcgagatc gccacgctcg atggggccgg gacgcatacc gccacctgca
     1621 tcctcgactg caaatggtcc tatggctcgt acgacctggc cggtccggag ggagcgtcga
     1681 cgggtgcctt cctcaccgat ctgagcgacc cggctgcacc caaaggtgtc ggccattgga
     1741 atgagaaatt gcccgccgac aagatccacg acgtcgtcga ggtcgcgccc gggcgggtgt
     1801 tgaccgcgtc ggcgcccttg atgttcgtcg atgcacggaa ggatccgctg cgtcccaccc
     1861 ttctcgctaa gggcacgaac atcgatcacc gattgcactc ggtggaatgg ccgcgcggcg
     1921 gcaaggacag attcatcatg agctcgttcg aaacgaatgc cacaccgcgg tgcgaagccg
     1981 gcgtcggcga gttcgcgacg tgggacgcat cgaagtggca gaagacccat acgttcacgg
     2041 tcatcgacga gttccacatc acgaatggga cctacaccga tggtcggccc gctgcgaacg
     2101 gaacagggct cggttgctca ccccattggt tccaggcaca ttccgacttc cacgacggtg
     2161 gggttgtggc cgcagggttc tacgaccacg ggacgcgctt cctcgagatc tcgagcgcgg
     2221 gcaagatcga ggagatcggt tacttcctcc cgtggggtgg cgcgacctcg gccgcctact
     2281 gggtaaccga cgaggtcgtc tactcgatcg actacgagcg gggcttcgac gttctgaggt
     2341 tcaagcctta gcgttctgag ctcttaatgc ctccgcgagg tccaggatcg aaccttgtgc
     2401 cgcgatctct tgctggcgct tagcgaggtt gctgttcgtc ggtgcgatca ggctcatccc
     2461 aatcaaccac agcggaagtt cagataggta gggccacaga tccttggtga tgaagcacgc
     2521 aacgaaggcc agcatgaggg gcatctcgct gagcccgaac ccgctgaaga acgcagttct
     2581 gtaggacgaa gccagcgact cgtttgactc aagtgcgagc ggtcgccgtg tcgaccagtg
     2641 ggcgcctgct atccccacta cccccgcccc gagcacgacc atcagccatg gcacatcgat
     2701 ccgcccgata ccaggatcga tcttgtacag gatgaagagg atcaggaatc ccgcgatgat
     2761 ctgaacccgg aaaaggatct tgagcgcagc cagaccgtcc tgcaccctcc gaccctaacc
     2821 aggggtgggc aggaagtcgt gggagctccc cgaatccttc tgggatgtac cggcgcctaa
     2881 ccctcctcgg aactctcgcc gccctcggca tgctcgtcac tgccgtgccc ccggccattg
     2941 gtcaggagac ctgcgagccg tgggagaggc gcacgatcgc cgaaggtctc gaccgcatcg
     3001 agaacctcat tccggacggt aagggcggga tgctgatcag cgccgggccg cgcaatgcga
     3061 tcgaacgggt gacccccgac ggcgaagtca ccacggccta cgaagacgtc cccgggcccg
     3121 gcggtctgcg catcatcggg cacaccctct acgcggtgac gaacgggaac atcgtcgacg
     3181 gagtgacgga ttctccgaac gggaagatcg agacctttga cctgaagagc ggcgagcgca
     3241 acatctacag cgagggtcta acggctccga acggacttgt gatcg
//
LOCUS       DATMED010000107         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2007717, whole genome shotgun sequence.
ACCESSION   DATMED010000107 DATMED010000000
VERSION     DATMED010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007717"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VNP73_02690"
     CDS             <1..1126
                     /locus_tag="VNP73_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWL64857.1"
                     /translation="ASHFVPAKDIASAGLMSVMREFAREQDIEHFFDVGRGGIEHILL
                     PEEALTLPGDVVIGADSHSCTYGALGCFSTGVGSTDIAAVWATGEIWLRVPESMKIVY
                     EGRPGPWIVGKDLILTVIARIGDDGATYMAMEHTGSALQHLSIDSRLTLTNMAIEAGA
                     KSGIIPPDDRTLEYVGLRQGAVGRYRDFDVFLSDEDAEYAAVIDIDVSAIEPMVARPS
                     LPSKAIPVSQSERVKVDQVFIGSCTNARIEDLRIAAHVLGSERVAPGVRLMVIPATQR
                     VWQQANQEGLLDLFAQAGASVSTPTCGACLGGHMGVLGPGEVCVSTSNRNYVGRMGDP
                     TAQVYLTNPAVAMASAVAGELVHPSEVRANAPILEEVLVG"
     gene            1119..1610
                     /locus_tag="VNP73_02695"
     CDS             1119..1610
                     /locus_tag="VNP73_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWL64858.1"
                     /translation="MARAFKYGDNIDTDVIIPARYLTTSDEAELAQHALEDLDATFAT
                     QVKPGDVLFAGANFGCGSSREHAPIALRGSGVAAVVAESFARIFFRNAINRGLPILVC
                     PEAVRATNAGDSVEVDPTAGRIVNVTKDQTFYADPLPDFVTQIVDAGGLIPYVRRRLE
                     GVS"
     gene            1607..2500
                     /locus_tag="VNP73_02700"
     CDS             1607..2500
                     /locus_tag="VNP73_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWL64859.1"
                     /translation="MTRIVSVLAGDGIGPEVIEQAVKVLPSEYLVKRVTTGAERYLAT
                     GELLAADDLKTISESDALLFGAVGDPRVPDGILERGILLELRRMLDLYVNLRPFPDLG
                     VVFVRENTEGAYAGMGRRVDDQAVEVSVNTSTAIRRCANYAFDLARRRSGRLTLVHKR
                     NVMTYAGALWSEVVDEVAKQYVDVEVLYEHADAAAYHLVRDPGRFDVILTDNLFGDLL
                     SDLAAGLSGGLGKAASANLHPDVATRPTRCVGLFEPVHGSAPDIAGSGTADPTAAIEA
                     ARMLVDALEARGAEREEPVFT"
     gene            2497..2922
                     /locus_tag="VNP73_02705"
     CDS             2497..2922
                     /locus_tag="VNP73_02705"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HWL64860.1"
                     /translation="MTTSPYFDVCGYKVVSERLHGRASEIQAIVEVRIGHTCVRRSAT
                     GVGPVHALDNALRKCLTDEFPELKDHRLTDYKVSVVDAAEGTGAQVRVLISSTDGTET
                     WDAGCISENIIEASFEALCSSSIMGIMRTRSTRVSALTP"
     gene            2932..3756
                     /gene="pheA"
                     /locus_tag="VNP73_02710"
     CDS             2932..3756
                     /gene="pheA"
                     /locus_tag="VNP73_02710"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWL64861.1"
                     /translation="MDDAIVAYQGEAGAYSEEGAIALFPETEHKPYSSIRKVFEAVEV
                     GKVDFGLVPMDNSQAGSINETYDLFLKHGLHLVGETVVRVNHCLLGIPGTAMDELTEV
                     ISHPQAIAQCEEFLSALELKIRAEYDTAGAAKQIVDDKMTTTAAIASKRAAEIYGLEV
                     LAEKIQTYPDNYTRFGALSRAPDPLREPDKSSLVFGVGHVAGSLFRCLGAFAERHLSL
                     SKLESRPRAGRPWEYVFYADIEAPAHAPQMIEALAEVSEHATFTRLLGTYASGISR"
BASE COUNT          726 a         1242 c         1341 g          791 t
ORIGIN      
        1 ggcatcgcat tttgttccgg cgaaggacat cgcgtcggcc ggcctcatga gcgtgatgcg
       61 cgagttcgcc cgggagcagg acatcgagca cttcttcgat gtcgggcgcg gtgggatcga
      121 gcacatcctg ttgccggaag aggcattgac tcttcccggc gacgtcgtta ttggcgccga
      181 ttcccattcg tgcacgtacg gcgcgttggg ttgtttctcc accggtgtcg gctcgacgga
      241 tatcgcggcc gtgtgggcga ccggcgagat ctggctgcgc gttccggagt cgatgaagat
      301 cgtctacgag ggccggccgg gcccgtggat cgttgggaag gacctgatcc tgaccgtgat
      361 cgcgcgcata ggcgacgacg gtgcgacgta catggcgatg gagcacacgg gatcggcgct
      421 acaacatctc tcgatcgatt cccggctgac cttgacgaac atggctatcg aagcaggagc
      481 caagtcgggg atcatcccgc ccgacgaccg cacgctggaa tacgtcgggc tgcgtcaagg
      541 cgcggtcggt cgctacaggg acttcgacgt cttcctctcc gatgaagatg ccgagtatgc
      601 ggctgtgatc gacatcgatg tgtcggcgat cgagcccatg gtggctcggc cgtccttacc
      661 gtcgaaggcc atcccggtct cgcagtcgga gcgcgtgaag gttgatcaag tcttcatcgg
      721 ttcgtgcacg aacgcccgga tcgaggacct gcggatcgcc gcgcacgtgc tcgggagtga
      781 gcgcgtggcc ccgggggtgc gcctgatggt catccctgcg acgcagcgcg tgtggcaaca
      841 ggcgaaccag gagggcctgc tggatctctt cgcgcaggcc ggtgcaagtg tgtcgacgcc
      901 gacgtgtggg gcgtgtctcg gcgggcatat gggagtgctc ggcccgggtg aagtgtgcgt
      961 gtcgacgtcg aaccgcaact atgtcggcag gatgggtgac ccgaccgcgc aggtctatct
     1021 cacgaacccc gccgtcgcga tggcgtcggc cgtcgccggt gagctcgttc atccatcgga
     1081 ggtacgcgcc aacgcaccga ttctcgagga ggtcctcgtt ggctagggcc ttcaagtacg
     1141 gcgacaacat cgacaccgac gtcatcatcc cggcgcgtta cctcactacg tcggacgagg
     1201 ccgagctcgc gcagcatgcg ctcgaggatc tcgacgcgac gtttgcaacc caggtcaagc
     1261 ctggtgatgt tttgttcgcc ggcgcgaact tcggctgcgg ttcgtcccgt gagcacgcac
     1321 cgatagcgct gcgcggttca ggtgtggctg cagtggtggc cgagtccttc gctcgcatct
     1381 tcttccgcaa cgcgatcaat cgggggcttc cgatcctcgt ttgtcctgag gccgttcgtg
     1441 cgaccaacgc cggcgacagc gtcgaggttg acccaacggc cggccggatc gtcaacgtca
     1501 cgaaggacca gacgttctac gccgacccgc ttccggattt cgtcactcag atcgtcgacg
     1561 cgggagggct gatcccgtac gtgcgccgaa ggctcgaggg ggtgtcatga ctcggatcgt
     1621 ttcagtactc gcaggagatg ggatcggacc cgaagtgatc gagcaggcgg tgaaggtcct
     1681 gccgtccgaa tacctcgtca agagggtcac gaccggggcc gagcgttacc tcgcaacggg
     1741 tgaattgctg gccgccgacg atctcaaaac gatctcggaa tcggacgcct tgctgttcgg
     1801 agccgtgggc gatcctcgtg tgcccgacgg gatcctcgag cgagggatcc tcctcgagtt
     1861 gcgcaggatg ctggatctct acgtcaacct tcgccctttc cccgatctcg gcgtggtctt
     1921 cgtgcgcgag aacaccgagg gggcctacgc cggcatgggc cggcgtgtcg acgatcaagc
     1981 cgttgaggtc agcgtcaaca cgagcaccgc gatccgccgg tgtgcgaact acgcgttcga
     2041 cctggcgagg agacggagcg gcaggctgac gctcgtccac aagaggaacg tcatgaccta
     2101 cgccggcgcg ctgtggtcgg aggtcgttga cgaggttgca aagcaatacg tggacgtaga
     2161 ggtcttgtac gagcacgccg atgccgccgc ttatcacttg gttcgggatc ccggaaggtt
     2221 cgacgtcatc ctgaccgaca acctgttcgg tgacctgctg tcggacctcg cggccggact
     2281 gtccggaggc ctgggcaaag ccgcctcggc caatctccat cccgatgttg caacgcgtcc
     2341 cacacgctgt gtggggttgt tcgaaccggt ccacggttct gctccggaca tcgccggtag
     2401 tggaaccgcc gatcccaccg cagcgatcga agcggcccgg atgttggtcg atgccctcga
     2461 agctcgagga gcagaaagag aggagccggt gttcacgtga caacctcccc ctacttcgac
     2521 gtttgcggtt acaaggtggt gagcgaacgt cttcatgggc gcgcctcgga gatccaagcg
     2581 atcgtcgagg tgcgtatcgg gcacacgtgc gtgcgcaggt ctgcaacggg agtcggtccc
     2641 gttcacgcgc tcgacaatgc cctaaggaaa tgtctgacag atgagttccc cgagctgaag
     2701 gaccacaggc tgaccgacta caaggtgtcg gtcgtggatg ccgccgaggg cacgggggcc
     2761 caggtgcgcg tgttgatctc gtcgacagac gggaccgaga catgggatgc ggggtgcatc
     2821 tccgagaaca tcatcgaggc gtcgttcgag gcgctgtgct cctcgtcgat catgggcatc
     2881 atgcggactc gatcgaccag ggtctccgct ttaacccctt aacctgtgcg catggacgat
     2941 gcgatcgtcg cctatcaggg cgaggccggt gcttactccg aggaaggcgc gatcgcgctc
     3001 ttccccgaaa ccgaacacaa gccgtactcg agcatccgca aggtgttcga ggcggtcgag
     3061 gtcgggaaag tcgatttcgg tctcgtaccg atggataaca gtcaggccgg cagcatcaac
     3121 gagacttacg acctgttcct gaagcacggg ttgcatctcg tcggcgaaac cgttgttcgg
     3181 gtgaaccatt gcctactcgg tatcccaggt actgccatgg atgagctcac cgaagtgatc
     3241 tcgcatccgc aagcgatcgc acagtgcgaa gagttcctgt ccgccctcga gttgaagatc
     3301 cgtgccgagt acgacacggc cggcgccgcg aagcagatcg tcgatgacaa gatgacgacg
     3361 acggctgcga tcgcgtccaa gcgagcagcc gagatctacg ggctcgaggt tctcgccgag
     3421 aagatccaga cctatcccga caactacacg cgcttcggcg cactgtcccg cgctccggac
     3481 ccattgagag agcccgacaa gtcgtcgttg gtgtttggcg tgggccacgt tgccggttcg
     3541 ttgttccggt gcctgggtgc attcgccgag agacatttga gtttgtccaa gctcgagtcc
     3601 cgaccccgag ccggccggcc gtgggagtac gtcttctacg ccgacatcga ggccccggcc
     3661 cacgctccgc agatgatcga agctctagcc gaagtttcag agcacgccac cttcacgcgc
     3721 ctcctaggca cctacgcatc cggcatctcc cgctaaccag ttcttactgc tttctaagca
     3781 ttttggctta ggcgttctac ctagacatgc cccgtgcgtg aggagtggac tgtcgcggac
     3841 ggaagtgaac cgaccaactg ggaggtggtc tatgacgcga tcgatccggt tcttggcctt
     3901 ggcggcttgt ctcgccttag cggcagccgc ggcgcctctg ccgggagcat cagcgagcca
     3961 caacggcaat ttgaacgtac aggtcggcca gttctttggg gtgggcggcc ggtgcaacca
     4021 ggccaccttc gagggctgcc gggctgccga gtcgatgcgc ttcttggccc cgtcgctcag
     4081 ggtccatgag ggcgatgtga
//
LOCUS       DATMED010000108         6822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4301323, whole genome shotgun sequence.
ACCESSION   DATMED010000108 DATMED010000000
VERSION     DATMED010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4301323"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1113)
                     /gene="trxB"
                     /locus_tag="VNP73_02715"
     CDS             complement(184..1113)
                     /gene="trxB"
                     /locus_tag="VNP73_02715"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWL64862.1"
                     /translation="MPETDKRDVVVIGSGPAGLTAAVYAARANLQPLVIEGVEAGGQL
                     MLTTDVENYPGFVDGIMGPELMERMRKQAARFGTEYLTDNVTSVDLSSQPFTIKTSGD
                     TVRTRSLIISTGASAKMLGVPGEKELLGHGVSTCATCDGFFFKDQELLIVGGGDSALE
                     EAIFLTKFAAKVTVVHRRDSLRASKIMQDRALSNDKIGFIWDSVLTEISGNGKVAAAR
                     LKNVKTGAESNVDAGGVFVAIGHTPNTSLFEGQLELSGGYIVTEGEDTQTSVPGVFAA
                     GDVVDFRYRQAITAAGMGCMAAIDAERYLESLH"
     gene            complement(1173..2246)
                     /locus_tag="VNP73_02720"
     CDS             complement(1173..2246)
                     /locus_tag="VNP73_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWL64863.1"
                     /translation="MEHATEHEYVSASLGSFVDGTLSDEDALRVRSHLADCTSCASEL
                     AALHELRGLTGGELSELESRRLLAAVRNATRDSIAMPASVPAAVRPSPGRSRFAQALG
                     AVALLALATVGAYMTFAGNTDGSSQGAAEDAAGGAGQGDAVAEIRSGLEYAAAPVGGA
                     LEPADSEPEQLEPLTEDAGDTGATGATGGEAGTGIGRDSGGSGAGEGAAKTGGDERRD
                     RDFYSQDPRPFYVRRPGRISDSTLVRIGSYGLPLVDFTRFTAADAFDRQVAHLSQLAN
                     AAESPERAEQIADCGTAVLARPEPILPALATFGRYRGRTTLVLAFAWTDSEDGPLDRF
                     MVWTWNEGSCDEVPGYHAGFIQR"
     gene            complement(2246..2812)
                     /locus_tag="VNP73_02725"
     CDS             complement(2246..2812)
                     /locus_tag="VNP73_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWL64864.1"
                     /translation="MRPAAGAADESLVDLFLGGDEGAFEELMRRHENRIFSLALRMMG
                     DRAAALDVTQDTFITAWSRASSFRKDSAFGTWLYRIGINTAKDHLRKSRRVTLESTDV
                     LERKPASGDVAASVGTRLDVARALQELPEEYREAVCMHDLGGIPYEEIALLTDTPIGT
                     VKSRISRGRRRLAEILELQPASPTSKDG"
     gene            complement(2809..4260)
                     /locus_tag="VNP73_02730"
     CDS             complement(2809..4260)
                     /locus_tag="VNP73_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWL64865.1"
                     /translation="MPVDRETIGDRYVLEEVFTRGGMATVYRARDEVLARPVAVKVLN
                     SDLAADERFLDRFRTEALAAARLAHAHIVQTFDTGEDEGRHFIVMEFCGAGTLADIIE
                     REAPLDPARAVGLCLDVCDALSYAHRHDIVHRDVKPANVLIGPAGSLKVGDFGIAKAA
                     FGSDDLTTTGKILGTVTYLSPEQAGGHEPDARSDLYSLGVILYELLVGRPPFSAETHV
                     ATAMKHLREEPPPLRTQRAGVPRSLEEVVLRSLAKDPDDRFANADEMASSLRAATGAE
                     RATTVIPTTPRPRPGAGAPEEQGIGRILILIAAIVAIAIIVAYVVGDGGPLRNGSPTS
                     GEDGAERLRVGSVVDFDPYGEGQEEHREEVPLAADSNASTMWTTEDYQDSFELLNKPG
                     VGLVFDLEAAEEVASLEVVLGVPGVDLEVRAGNDPPGTQNDLELVAEVQNADNAVTVD
                     VNGTEARYWLVWITSLPGNGGGNAAIAEVRFLQ"
     gene            complement(4270..4677)
                     /locus_tag="VNP73_02735"
     CDS             complement(4270..4677)
                     /locus_tag="VNP73_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64866.1"
                     /translation="MRIFDERGVLVSWLIKIILVLGVLGVIVFDGASIMVNIFTLDSS
                     ANDTAIAVSLQVEPDQFGRNDQEVFDKTKAVIASDDTGAANAKVIRNGTVVDEEGNVH
                     VKLRRVATTLVVRYIAPLKKWTVATAEGSASTN"
     gene            complement(4710..6302)
                     /gene="murJ"
                     /locus_tag="VNP73_02740"
     CDS             complement(4710..6302)
                     /gene="murJ"
                     /locus_tag="VNP73_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWL64867.1"
                     /translation="MSLARATAVMSVGTVLSRVTGLLRLAAIAAALGVVESDRLTDTY
                     NLANTAPNIIYELFLGGILTSVFVPVFVELLEKEGRERAWEVASAIINVSVVILVAIT
                     TLFILAAPWIANIYAVRLEGSDQALQQEALTFLLRLFIPQIVFYGLTAVTAGLLNAHK
                     RFGAPMYTPILNNLGVIAVFLAFHQAYPLVTLRTATTTQLVIIGLGTTAGIALMAIAQ
                     LPFLRGLGRYRLNFSVRHPAIKKIARLSSFVVGYVIANQIGYLIVQWLANAQKGGYTA
                     YVWAFTFFMLPHGLFAVSLITALLPSMSEHAVNQRWDELRERVSIGVRATFLLVLPAA
                     VGYLVMGEPIVRLLLEHGVMTARSTRLVAGVLQFFVVGLVPFSLFQLFLRVFYAMQNT
                     KTPFLINCGAVALNTAINVPMFAWLGVRGLAAGHALAYIFGVVVQGRSLAKTIGGLDT
                     RRVVRSAVRMAMAAVIMGAFVWLLSRGVERIVSVDDLVGQIVVVLVPVAGGAVAYMAL
                     LRVFRIQELDYVRGLVARRGGR"
     gene            complement(6299..>6822)
                     /locus_tag="VNP73_02745"
     CDS             complement(6299..>6822)
                     /locus_tag="VNP73_02745"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HWL64868.1"
                     /translation="LRRNLLVAQSRLWITDPALLAGAESFAAETLQETEDELDKVRIG
                     GIEEIRLTSRRGEIPIEIFNGTDYDVAVNIKVLSADLRLNETLPEVLQPGRFRQLTVD
                     VSASSSGIFPVEVSVETPDGSYEIDRKSITVRSTEFNEIALVITLGALAFLIVFYVMR
                     WFRRREPEDEAAA"
BASE COUNT         1226 a         2173 c         2163 g         1260 t
ORIGIN      
        1 agttggtccg ccggacgggg aacgtcattc cgtcgtcgac gaccggttct ctgaaggtcg
       61 gcgacccacc ggccgaacct gccggcagca aaagggtcac gcacgacaag atcagtgcga
      121 cgcgtgcgat cttcatctgg atgaggctat aggccgcgcg caccgtgcgg cacgcgaacg
      181 ggtttagtga aggctctcca ggtaccgctc ggcgtcgatc gccgccatgc atcccatccc
      241 tgccgcggtg atcgcctgcc ggtaccggaa gtccacgaca tctcccgccg cgaatacccc
      301 gggcaccgag gtttgcgtgt cttcaccctc cgtcacgatg tagccgccgg agagctcgag
      361 ctgtccttcg aagagtgagg tgttcggcgt gtgaccgatg gcgacgaaaa caccgcccgc
      421 atcgacgttg ctctccgccc ccgtcttcac gttcttgagt cgcgccgcgg cgacctttcc
      481 gttgcccgag atctcggtca gcaccgagtc ccagatgaat ccgatcttgt cattcgacag
      541 ggctcggtcc tgcatgatct tcgacgcgcg gagcgagtcg cgtcggtgga cgaccgtgac
      601 cttcgcggcg aatttcgtaa ggaagatggc ttcctccaaa gccgagtcac ctccaccgac
      661 gataaggagc tcctggtctt tgaagaagaa gccgtcacag gtcgcgcacg tagatacgcc
      721 gtggccgagg agctctttct cacccgggac ccccagcatc ttggccgatg cgccggtcga
      781 gatgatcagc gacctggttc tcacggtgtc gccggaggtc ttgatcgtga aaggttgcga
      841 tgaaagatct acggacgtga cgttgtcggt tagatactcg gtaccgaaac gcgcggcctg
      901 cttgcgcatc cgctccatca actccgggcc catgatcccg tctacgaagc ccgggtagtt
      961 ctcgacgtcg gtcgtcagca tcaattgccc gcctgcttcg acgccttcga taaccagtgg
     1021 ctgcaggttc gctcgcgctg cgtagaccgc ggccgtcaga ccggcgggac cggatccgat
     1081 gacgaccacg tctctcttgt ctgtctctgg catgttcaca acgctatcaa cgacctcgcg
     1141 gcgcgctttg tttccggagc tcgcgctccg tctcagcgtt ggatgaaacc ggcgtggtag
     1201 ccgggcactt catcgcagga gccctcgttc cacgtccaca ccatgaagcg atcgaggggg
     1261 ccatcctcac tgtcggtcca ggcgaaggcg agcacgaggg tcgtgcgtcc ccggtatctg
     1321 ccgaaggtcg cgagcgccgg gaggatgggc tcgggccgcg ccagcacggc cgtcccacag
     1381 tcggcgatct gctcggctcg ctccggcgac tcggcggcat tggccagctg actcagatgg
     1441 gcgacctgac gatcgaaggc atccgcagcg gtgaagcgcg tgaagtcgac caaggggagc
     1501 ccgtatgagc cgatccgcac cagggtgcta tcgctgatcc ggcccggccg gcggacgtag
     1561 aagggcctcg gatcctggct gtagaagtcc cggtcccgcc gttcgtcccc accggttttg
     1621 gcagctcctt cgcccgcccc gcttcctccg ctgtctcgtc cgataccggt gccggcttct
     1681 ccgccggtgg cgccggtggc gccggtgtcg cccgcgtcct cggtcaacgg ctcgagctgt
     1741 tccggctcgg agtcggcggg ctcgagcgct cctcctacgg gagcggccgc gtactcgagg
     1801 ccggagcgga tctcggcgac ggcatcgcct tgtcccgcac cccccgcggc gtcctcggcc
     1861 gccccctggc tcgacccgtc ggtgttcccg gcgaaggtca tgtaggcgcc gaccgttgcg
     1921 agcgcgagca aggccacggc gccgagggcc tgggcgaagc gagagcggcc cggcgacggc
     1981 ctgacggcgg cggggacact tgcgggcatg gcgatcgagt cccgggtggc attgcgtacc
     2041 gcggcgagca ggcgccggct ctccaactcg ctcagctccc caccggtcaa ccctcttagc
     2101 tcgtgaaggg cggcgagctc gctcgcgcaa ctcgtgcaat cggcgaggtg agatcggacg
     2161 cgtagggcgt cttcgtccga cagagttccg tcgacgaacg agccgaggga ggccgatacg
     2221 tattcgtgtt ccgtagcgtg ttccatcatc cgtccttaga cgtgggggaa gcgggttgaa
     2281 gttccaggat ctcggcgagt cggcggcggc cgcggctgat cctcgatttc acggttccga
     2341 tcggcgtatc ggtcagcagg gcgatctcct cgtacggtat gccgccgaga tcgtgcatac
     2401 agacggcctc ccggtattcc tcgggaagct cctgcaacgc acgcgcgacg tccagacgtg
     2461 tcccaaccga agctgcgacg tctccggaag ccggtttcct ctcgaggacg tctgtggact
     2521 cgagggtcac ccggcgactc ttgcgcagat gatctttagc ggtgttgatg ccgatgcgat
     2581 agagccaggt accgaaggcc gagtctttcc ggaacgacga cgcgcggctc catgcagtga
     2641 tgaaggtgtc ctgtgtcaca tccaacgccg ccgctcgatc acccatcatc cgcaacgcga
     2701 gcgagaagat ccggttctcg tggcggcgca tgagttcttc gaacgctccc tcgtctcctc
     2761 cgaggaacag gtcgacaaga ctctcgtctg cggctccggc ggcgggcctc actggaggaa
     2821 tcgaacctct gcgatggctg cgttcccccc gccgttaccc gggaggctcg tgatccacac
     2881 gagccagtaa cgcgcttcgg tgccgttcac gtccaccgtg acggcgttgt ccgcgttctg
     2941 cacttccgcg acgagctcca ggtcgttctg cgttccggga gggtcattcc cggcgcgtac
     3001 ttcgagatcg acccccggca caccgagaac gacctcgagt gaggcgacct cctcagctgc
     3061 ttctagatcg aagacgagcc cgacgccggg cttgttcagg agctcgaacg agtcctgata
     3121 gtcctccgtg gtccacatcg tcgacgcgtt cgagtcggcc gcgagcggca cctcttcacg
     3181 gtgttcttcc tgcccctccc cgtaggggtc gaagtcgacc accgatccga cgcgcagcct
     3241 ctcggccccg tcctcgcccg acgttggaga gccgttccgt aggggtccgc cgtcaccgac
     3301 cacgtaggca acgatgatgg cgatcgcgac gatcgcggcg atcaggatca ggatgcggcc
     3361 gatgccctgt tcttcggggg ccccggcgcc cggccgcggc cgtggcgtag tggggatgac
     3421 cgtagtcgct cgttcggccc cggtcgcagc tcgtagtgaa gatgccatct cgtcggcatt
     3481 tgcgaagcga tcgtccgggt ctttggcgag ggaacggagt acgacctcct cgagcgatcg
     3541 cggcaccccc gccctctggg tccgaagcgg aggtggttct tcccgtagat gtttcatcgc
     3601 ggtggcaacg tgcgtttcag ccgagaaggg cggtctcccc accaagagtt cgtacaggat
     3661 cactccgagg ctgtagaggt ccgagcgcgc gtcgggctcg tgtccgccgg cttgttcggg
     3721 cgataggtac gtgaccgttc cgaggatctt tcctgtagtg gtcaggtcat cggacccgaa
     3781 cgcggccttg gcgatcccga agtccccaac cttcaacgat cccgccggac cgatcagaac
     3841 gttggccggt ttcacgtctc gatggacgat gtcgtggcgg tgggcatagc tcaacgcatc
     3901 gcagacgtcg aggcacagac cgaccgcccg cgcggggtcg agaggtgcct cacgctcgat
     3961 gatgtctgca agcgttccgg ccccgcagaa ctccatgacg atgaagtggc gaccctcgtc
     4021 ctcgccggtg tcgaacgtct gaactatgtg tgcgtgcgca aggcgagccg cggcgagcgc
     4081 ttcggttctg aacctgtcca ggaatctctc atcggcggcg aggtcgctgt tcaggacttt
     4141 gacggcaacg gggcgggcca gaacctcgtc ccgggcgcgg tacaccgtgg ccatgccccc
     4201 tcgggtgaat acctcttcca ggacgtagcg atccccgatg gtctcgcgat caaccggcat
     4261 gtggggatgc tagttcgtgg atgcggagcc ttcggcagtg gcgaccgtcc acttcttcag
     4321 gggggcgatg tatctgacga ccagtgtggt tgcgaccctg cggagcttca cgtggacgtt
     4381 gccttcttcg tcgacgacgg tcccgtttcg gatcaccttt gcgtttgctg cgcccgtgtc
     4441 gtccgaggcg atgaccgctt tcgtcttgtc gaacacctct tggtcgttcc taccgaactg
     4501 atcgggctcg acctgcaggg acacggcgat agcggtgtcg ttcgcgctgg aatccagtgt
     4561 gaatatgttg accatgatcg aagcgccgtc gaagacgatt accccgagga cccccaagac
     4621 caggatgatc ttgatcagcc aggagaccaa cacgccccgc tcgtcgaaga ttcgcatcgg
     4681 tttgattatc ggcttgtgag ggccgttact caacgacccc cgcggcgcgc gactaaccca
     4741 cgtacgtagt ccagttcctg gattctgaag acgcgcagga gcgccatgta ggcgacggcg
     4801 cctcccgcga cgggcactag gacgacaacg atctggccga cgagatcatc gaccgagacg
     4861 atccgttcca cacctcgtga caacagccag acgaaagccc ccatgatcac ggcagccatc
     4921 gccatccgaa cggcgctcct tactacgcgt cgagtgtcga ggcccccgat ggttttcgcc
     4981 aacgatcgac cctgaaccac aaccccgaat atgtacgcga gagcatggcc ggcggcgagt
     5041 ccgcggaccc ccagccaggc gaacatcgga acgttgatag cggtgttcag cgcgaccgcc
     5101 ccgcagttga taaggaaagg cgtcttggtg ttctgcattg cgtagaacac gcgcaggaac
     5161 agctggaaaa gggagaaggg caccagaccg acgacgaaga actgcaacac acccgcgacg
     5221 agcctcgtcg agcgagcggt cataacaccg tgctccagaa gcagcctgac tatcggttca
     5281 cccatgacga ggtatccgac cgcagccggg agcaccagaa ggaacgtggc gcggactccg
     5341 atcgacactc gctcgcggag ttcgtcccag cgttggttga ctgcgtgctc gctcatcgag
     5401 ggaaggagag cggtgatcaa agaaacggcg aacagaccgt gcggaagcat gaagaaggtg
     5461 aatgcccaca cgtaagcggt gtaaccgccc ttctgcgcgt tggccaacca ttgcacgatc
     5521 aggtagccga tctgattcgc gatcacataa ccgaccacga aggacgaaag acgcgcgatc
     5581 ttcttgatcg cagggtgacg gaccgagaaa ttcagtcgat agcggcccaa cccacgcagg
     5641 aacgggagct gcgcgatcgc catcagagct atgccggcgg tggtgccgag gccgattatc
     5701 actaactggg tggtggttgc cgtccggagt gtgaccaacg gatatgcctg gtggaaggcg
     5761 aggaacactg cgataacacc aaggttgttc aagatgggcg tgtacatggg cgcgccgaat
     5821 cgcttgtgcg cgttcagcag tcccgccgtc acagccgtga gcccatagaa gacgatctgc
     5881 gggatgaaga gccgtaggag gaacgtcagc gcctcctgct gcagagcttg gtccgagccc
     5941 tcgaggcgca cggcgtagat gttggcgatc cagggggccg cgaggatgaa gagcgtcgtg
     6001 atcgcaacga ggatcaccac ggacacattg atgatcgcgc tcgcgacctc ccacgcccgt
     6061 tcgcgacctt ctttctccag cagttcgacg aataccggga cgaagacgga ggtgaggatc
     6121 cctcccagga acagctcgta gatgatgttc ggggcggtgt tggcgaggtt gtaggtgtcc
     6181 gtgaggcggt cggattcgac tactcccagc gccgccgcga tagcagccag tctcagcagc
     6241 ccggttaccc gcgacaaaac ggtaccgacc gacataactg cggtggctcg ggccaggctc
     6301 aagcagcggc ctcatcttct ggttcgcgtc tgcggaacca cctcatcacg tagaacacga
     6361 tcaggaacgc gagcgctccg agagtgatga ccagcgcgat ctcgttgaat tctgtagagc
     6421 ggacggttat cgacttgcga tcgatctcgt acgagccgtc aggggtctcg accgaaacct
     6481 caacggggaa gatcccgctc gagctcgcgc tgacatcgac cgtcagctgg cggaagcgtc
     6541 cgggttgcag aacctcgggc agggtttcgt tcaagcgcag atcggccgag agaaccttga
     6601 tgttcacggc gacgtcgtag tcggtcccgt tgaatatctc gatggggatc tctccacgcc
     6661 gggatgtaag ccggatctcc tcgatccccc ctatccggac cttgtcgagc tcgtcctcgg
     6721 tttcttggag cgtttcggcc gcgaaagact cggcccccgc gaggagtgcc ggatcggtga
     6781 tccacaaccg gctctgggcg acgagcaggt tccgtcgtaa tc
//
LOCUS       DATMED010000109         4140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4301348, whole genome shotgun sequence.
ACCESSION   DATMED010000109 DATMED010000000
VERSION     DATMED010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4301348"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..635
                     /locus_tag="VNP73_02750"
     CDS             30..635
                     /locus_tag="VNP73_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HWL64869.1"
                     /translation="MSSRPEMIRRAREVEGPTPKPLKIVVTGPFSAGKTTLIKAISEV
                     TVLGTERDVSDASKSVKSRTTVAMDFGRITFADDLSLFLFGTPGQRRFEVMWDVLAEG
                     MIGFILMVSAADEDSIADSLHILEHFRSRADVPFVIGVSHLDRAEADSSDLQEEIHKR
                     LGLNESTQVVPCDPRDREQVKQLVLRVLTAALERLENPVDV"
     gene            1018..2634
                     /gene="gspE"
                     /locus_tag="VNP73_02755"
     CDS             1018..2634
                     /gene="gspE"
                     /locus_tag="VNP73_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HWL64870.1"
                     /translation="MAREQATQRGRSLGRVVIEMGFATEGGLVAILAEQLGLEFIDLA
                     DASVDASAVALVPETTARRHNCIPVGFDEDQRLILAMADPANVVAVDDIRAMAKRDVR
                     TVVATKADVVAALNRYYRLDRTAETLAEEAAEDVAADGGVLEVDEEARAEDAPIIKLV
                     NLLITQAVNDRASDIHVEPGERSLKVRYRIDGVLHEVMSPPKSVQAGITSRLKIMADI
                     NIAERRIPQDGRIGLKVQGKGIDIRVATLPTVYGEKIVMRLLDKSAVMMQLSDLGFQP
                     DNFKRFEESYKKPYGMILVTGPTGSGKSTTLYATLNILNQPHVNVITVEDPVEYRLPG
                     VNQVQTNNKAGLTFASALRSILRSDPDIVLIGEIRDRETAQIAVEAALTGHLVLSTLH
                     TNDAPSSMTRLVEMGIEPFLVASSLDCAVAQRLARKLCPKCKESYVPTEDELIAAKFP
                     YDPAAELPKLYKAIGCSACGGTGYKGRLAVHEIMTVTEDIERLVAERASAEEIGKSAR
                     SQGMSTLRDDGMSKVRQGVTSIEEILRVVV"
     gene            2659..3915
                     /locus_tag="VNP73_02760"
     CDS             2659..3915
                     /locus_tag="VNP73_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWL64871.1"
                     /translation="MAYTGKPEAPTTPETQQPTYTPTPVTPSVEPVDPMQGDDLPLVE
                     APAPVEAEAPAPGEVEDEEKEPNLGEFLSAVIDRGASDLHLTGGLPPMIRVHGELSPL
                     KGYRKLLPKDLQELIYAMLTQKQREQFENNQELDLSYALPGRARFRVNVFQQRDAMGS
                     VMRLIPYGIKTLDELGLPQAVKQFADLRRGLILVTGITGSGKSTTLAGLIDIINTERA
                     DHIMTVEDPIEFLHRHKKCIVNQREVGSDTQSFSNALKYVLRQDPDVILVGEMRDLET
                     ISAALTAAETGHLVFGTLHTQDAPQSIDRVIDVFPPHQQQQIRVQLAGTIGGVISQQL
                     VPTVDGKGRAVACEILVATPAVRNLVREGKTHQIYTSMQAGGKHGMQVMDQHLAELVK
                     KNVVTYEMALERAHHLEEFNRLAGRG"
     gene            3945..>4140
                     /locus_tag="VNP73_02765"
     CDS             3945..>4140
                     /locus_tag="VNP73_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWL64872.1"
                     /translation="MADSYAYRVRTRDGRVIDGKMDADGESAVASKLRAQGMTPIQIK
                     QEASVSMKMEIKIFPGKVKLK"
BASE COUNT          896 a         1241 c         1287 g          716 t
ORIGIN      
        1 aggggctcta aacaggagag agaggaacca tgtcttcacg tcctgagatg atccgcaggg
       61 cgcgggaagt cgaaggtccc acgcccaaac cgctcaagat cgttgttacc ggccccttct
      121 cggccgggaa aacgacgctg atcaaggcga tcagcgaggt aactgttctc ggcaccgaac
      181 gtgacgtatc ggatgccagc aagtcggtga agagccgaac gacagtcgcg atggactttg
      241 gacgcatcac attcgccgat gacctgtctc tgttcctctt cggaacgcct ggtcaacgca
      301 ggttcgaagt gatgtgggac gttctcgccg aaggcatgat cggattcatc ctgatggtca
      361 gtgccgccga cgaggattcg atcgcagact cgctgcacat cctcgagcac ttcagatccc
      421 gcgccgatgt cccgttcgtg atcggagtga gtcacctcga cagagcggag gcagacagct
      481 ccgaccttca ggaggagatc cacaagcgac tgggcttgaa cgagtcgacg caggtcgtgc
      541 cctgcgatcc gcgcgatcgc gaacaggtca aacagttggt gctgcgggtt ctgacggccg
      601 cgctggagcg actagaaaac cccgtggacg tgtgatgcgg ttgtccgaag gctggaaacg
      661 cgcctttgac cgcttacgcc gcaaaccggc agaagcagga caaagtggcc accaggaagc
      721 acgagtgcgg atacaccttg cggacggccg cacggtggat atccctcgtg gtcgaagggt
      781 cgacagactc gtagaacacc tactcgcgga tctcgatagg ggtgtgaagt aacccaaata
      841 gggttgagtt tgtcgcgcgc gtccctttag tgtggcctcc gagggccgat aatctgacct
      901 atggggcaag ctaagacgcg cgccggcgta gccgacgtat cgacgcaatc taggccgggc
      961 ggccagctgg gcgaattact cctgggccga ggcctgatca ctgaagagca gctcgagatg
     1021 gcgcgcgagc aagcaacgca gcgcggccgg tcactggggc gcgtcgttat cgagatggga
     1081 ttcgccaccg aaggcggact ggttgcgatc cttgccgagc agctcggtct cgagttcatc
     1141 gacttggccg acgcttccgt cgacgcctcg gcggtggcct tggttcccga gaccacggca
     1201 cgacgacaca actgcatccc cgtcggcttc gacgaagacc aacgcttgat ccttgcgatg
     1261 gccgatccgg cgaacgtcgt ggcggtcgac gacatccgcg cgatggcgaa acgggacgtt
     1321 cgtaccgtcg tcgcaacgaa ggccgatgtc gttgcggccc tcaaccgtta ttaccgcctc
     1381 gaccgaaccg ccgagaccct ggccgaagag gctgcggagg atgtcgccgc agatggcggg
     1441 gtcctagagg tcgacgagga agcgcgtgcg gaagacgcgc cgatcatcaa actcgtcaac
     1501 ctcctgatca cccaggctgt gaacgaccgc gcatccgaca tccacgtcga gccgggggaa
     1561 cgctcactca aggtccgcta caggatcgac ggtgttctcc acgaggtgat gagtccgccg
     1621 aagtccgtgc aagcggggat caccagccgg ctcaagatca tggccgacat caacatcgcg
     1681 gagcgacgga tcccgcagga cggccgtatc ggtttgaagg tccagggaaa ggggatcgac
     1741 atccgcgtcg cgaccctccc aactgtgtac ggcgagaaga tcgtcatgcg actgctcgac
     1801 aagtcggcag tgatgatgca gctgtccgac ctcgggttcc aacccgacaa cttcaagcgt
     1861 ttcgaagagt cgtacaagaa gccctacggg atgatcctcg tcaccggtcc taccggttcg
     1921 ggcaagtcga cgacgctcta tgcgaccttg aacatcctca accaacctca cgtgaacgtg
     1981 atcacggtcg aggacccggt cgagtatcgg ttgccgggtg tcaaccaagt gcagacgaat
     2041 aacaaagctg gtttgacgtt cgcttccgcg ctgcgttcga tcctgcgatc cgaccccgac
     2101 atcgtcctca tcggtgagat ccgggaccgt gagacggcgc agatcgcggt cgaagcggca
     2161 ctgacgggtc acttagtgct gtcgaccctt cacacgaacg acgcgccctc gtccatgacc
     2221 cgtttggtgg agatgggcat cgagccgttc ctcgtcgcct cgtcgctgga ttgcgccgtc
     2281 gcccagaggc ttgcccggaa gctttgtccc aagtgcaaag agtcatacgt ccccaccgag
     2341 gacgagttga tcgcggccaa gttcccttac gaccctgctg cagagctccc caagctctac
     2401 aaagcgatcg gttgctcggc ctgtggaggg accggctaca agggccgcct tgccgtccac
     2461 gagatcatga cggttactga ggacatcgaa cggctggtgg ccgaacgagc gtcggccgaa
     2521 gagatcggga agtctgctcg atcgcagggg atgagcacgt tgcgcgatga cgggatgtcg
     2581 aaagtcagac agggagttac gtcgatcgaa gagatccttc gagtcgtcgt ttaatagtca
     2641 taggaaggta gtggagagat ggcttacacc ggaaaacccg aggcccccac gacgcctgag
     2701 acccaacagc cgacgtacac gcccacaccg gtgacaccgt ccgtcgagcc cgtcgacccg
     2761 atgcagggcg acgacctgcc cttggtagag gcgccggcgc cggtagaggc agaggcaccg
     2821 gccccgggcg aggtcgaaga cgaggagaag gagccgaacc tcggcgagtt cctctcggct
     2881 gtgatcgatc ggggagcgtc ggaccttcac ctgacagggg gtctgcctcc gatgatccgt
     2941 gtgcacggag agctttcgcc gctaaagggc taccgcaaac tcctaccgaa ggatctccag
     3001 gaattgatct acgcgatgct cacccagaag caacgcgagc aattcgagaa caatcaagag
     3061 ctcgacctgt cctacgcgct tccgggcaga gcgcgcttca gggtgaacgt cttccagcag
     3121 cgcgacgcta tgggttctgt catgcgactg atcccctacg gcatcaagac cctggatgag
     3181 ctgggccttc cgcaggcggt taagcagttc gccgacctcc gcaggggttt gatcctcgtt
     3241 accggtatca ccggttcggg taagtcgacg acgctcgccg ggctcatcga catcatcaac
     3301 accgagcggg cggaccacat catgacggtc gaggacccga tcgagttcct tcaccgtcac
     3361 aagaagtgca tcgtcaacca gcgtgaggtc ggaagcgata cgcagagctt ctcgaacgcc
     3421 ctcaaatacg tcctgcgaca ggaccccgac gtcatcctcg taggtgagat gagagacctc
     3481 gagacgatct cagcggctct gaccgctgcc gagaccggcc acttggtgtt cgggaccttg
     3541 cacacgcaag atgcacccca gtcgatcgac cgtgtgatcg acgttttccc cccgcatcag
     3601 cagcaacaga tccgagtgca gctcgcagga acgatcggag gagtcatctc ccagcaactc
     3661 gtgcctacgg ttgacgggaa gggccgagcc gtagcctgcg agatcctcgt tgcaacgcct
     3721 gcggtgagga acctcgtgcg agagggaaag acccaccaga tctacacctc gatgcaggcc
     3781 ggtggaaagc acgggatgca ggtcatggac cagcaccttg ccgagctggt gaagaagaac
     3841 gtggtcactt acgagatggc tctcgagcgc gctcaccacc tcgaagagtt caaccgcctg
     3901 gccggtaggg gatagattga agacctctag aagggaggcc tcgcatggct gattcgtatg
     3961 cctaccgcgt acggacccgc gacggccgag tgatcgacgg caagatggac gccgacgggg
     4021 aatcggcggt ggcttcgaag ctgcgcgccc agggcatgac gccgatccag ataaagcaag
     4081 aagcatcggt ctccatgaag atggagatca agatcttccc cggcaaggtc aagctaaagg
//
LOCUS       DATMED010000110         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5072329, whole genome shotgun sequence.
ACCESSION   DATMED010000110 DATMED010000000
VERSION     DATMED010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5072329"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VNP73_02770"
     CDS             <1..506
                     /locus_tag="VNP73_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWL64873.1"
                     /translation="WAALGFGALLGLRFLAIGFSGLTYAFTTHPSDLVQGFARHGLDY
                     RLVHAPVLGLVLFPSFLQLGKDISTTQRIRDLGADLHPIARKWASFKHLAFSMLLLGL
                     RRGQTQAMALDIRGYGAHKTRTFMRPIPSSVAGRVFGWTCFVLAVAYLVFQLGNLAGH
                     MQWDLGG"
     gene            690..1379
                     /locus_tag="VNP73_02775"
     CDS             690..1379
                     /locus_tag="VNP73_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWL64874.1"
                     /translation="MYHEITVWTRGIIMDKEARDIVNCLASGAEKAGYYAQFISDYID
                     DPDRTNCLVHKYARFGDEPIPDRFVYENANPDWVVLVEETLVKAANFFRGTPDGEAVL
                     VVNSAREPEYLLKFLPDYMLAKLKKLVVVDAVSLAEQEGSSPWMFVRDLAQLAYDRTS
                     TEGATERSDPGIGVAAPLLGALVAATGVLPLDTVRETVADQEAFTRGAEHHTVLDYGP
                     SQVREAAAQPI"
     gene            1404..>2336
                     /locus_tag="VNP73_02780"
     CDS             1404..>2336
                     /locus_tag="VNP73_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWL64875.1"
                     /translation="MSVKGTTIPPRLRLPLAGVTPAPTEEEGQRLFPTGNFRFYRPVY
                     KDKTPPCNHACPTGENIQKYLDFVKHERFLDGYLTIMEDNPMPSVTGRVCYHPCEGAC
                     NRKDHDEPIGIRNVERFLGDYGLALKDDPTKDSLPSTNGKSVAVVGSGPGGLGAAYHL
                     RRLGYKVTVLEALSKPGGMLRGGIPHWHLPEDVLNAEVAKIEDLGDIEIKCNTRVGTD
                     VSWDDLKNYDACFLAVGQDVGRKLPVEGNDLRGVTGGLEFVREAGFNKPVKVGKKVIV
                     VGGGNTASDAARSALRLGADEVTIVSLEGPDELLI"
BASE COUNT          428 a          753 c          756 g          399 t
ORIGIN      
        1 cctgggcggc gctcggattc ggcgcgctgc tgggcttgag gttcttggcg atcggcttct
       61 ccggcctcac ctacgccttt acgacccacc cgtccgacct cgtgcagggg ttcgcccggc
      121 atgggctcga ctaccggctg gtgcacgcgc cggtgctcgg gctcgttctc tttccctcgt
      181 ttcttcagct gggcaaggac atctctacga cccagcggat cagggacctc ggggccgacc
      241 tgcaccccat cgcccgcaaa tgggcgtcgt tcaagcatct cgccttctcg atgctcttgc
      301 tgggcctgcg gcgcggccag acgcaggcca tggcccttga cattcgcggt tacggagccc
      361 acaagacgag gaccttcatg aggccgatcc cctcctcggt ggccggtcgg gtgttcgggt
      421 ggacctgctt cgtgctggcg gtcgcttacc tcgtttttca gctggggaat cttgcgggcc
      481 acatgcagtg ggacctcgga ggctgagggt aaagggccat ccgaacgggt ccccgactcc
      541 tggggttggg gaactccgag gggggtagcc tgacaggaga tcaaccacaa aggttcggtc
      601 cgaaagggta tactgtatgc gatttccggt ggccgccctc ggggccggct cggccggacc
      661 gcagaccgcc aagggggagg tcagccagga tgtaccacga gatcacggtg tggacccgag
      721 gcatcatcat ggacaaggaa gcccgtgaca tcgtcaattg ccttgcgtcg ggagcagaga
      781 aggccgggta ctacgcccag ttcatcagcg actacatcga cgaccccgat cgcaccaact
      841 gcctggtcca caagtacgcg cgcttcggcg acgagccgat ccccgatcgt ttcgtctacg
      901 agaacgccaa tccggactgg gtcgtgctcg tcgaggagac gctggtaaag gctgcgaact
      961 tcttccgcgg cactcccgac ggagaggccg tgctcgtcgt caactcggcc cgcgagcccg
     1021 agtacctgct caagttcttg cccgactaca tgctggcgaa gctgaagaag ctcgtcgtcg
     1081 tggacgcggt gtcgcttgcg gagcaggagg gaagcagtcc ctggatgttc gtgcgcgatc
     1141 tcgcacagct cgcgtacgac cgaacctcca ccgagggagc taccgagcga agcgatcccg
     1201 gaatcggcgt tgccgcacct ctgctcggcg cgctcgtcgc cgcaaccggc gtactgccgc
     1261 tcgacaccgt gcgggagacg gtcgcagacc aggaagcgtt cacgagaggt gccgagcacc
     1321 acacagtgct cgactacgga ccatcgcaag ttcgcgaggc tgccgcgcaa ccgatctagg
     1381 cataccgact ggggggtacc tgcatgtcag tgaagggaac gaccatcccg cctcggctcc
     1441 gtctgccatt ggcgggcgtg actccggcgc ctacggaaga agaagggcag cggctctttc
     1501 ccaccggcaa cttccgcttc tatcgcccgg tgtacaaaga caagaccccg ccgtgtaacc
     1561 acgcctgccc cacgggcgag aacattcaga agtatctcga cttcgtaaag cacgagcggt
     1621 tcctggacgg ctatctgacg atcatggagg acaaccccat gccgtcggtg accgggcgcg
     1681 tctgctacca cccctgcgag ggtgcgtgca accgcaagga ccacgacgag cccatcggca
     1741 tccgcaacgt cgagcgcttc ctcggcgact acgggctagc gctgaaggac gaccccacca
     1801 aggattcgct gccctccacg aacggtaaga gcgttgcggt ggtgggatcg gggccgggcg
     1861 ggctcggcgc cgcatatcac ctgagacgac tcggctacaa ggtgaccgtc ctcgaggccc
     1921 tgtcgaagcc gggcggaatg ttgcgcggcg gaattccaca ctggcacctt cctgaagacg
     1981 tgctcaacgc cgaggtcgcc aagatcgaag acctgggcga catcgagatc aagtgcaaca
     2041 cgagggtcgg caccgacgtg tcgtgggacg atctcaagaa ctacgacgcc tgcttccttg
     2101 ccgtcggcca ggacgtcggc cgcaagctgc ccgtcgaggg gaacgacctc cggggcgtga
     2161 cgggaggact cgagttcgtc cgcgaagccg gtttcaacaa gccggtgaag gtgggcaaga
     2221 aggtgatcgt cgtcggcgga gggaacaccg cgtcggacgc cgctcgctcc gcccttcgtc
     2281 tgggagccga cgaggtcacg atcgtgtcac tcgagggacc ggacgagctg ctcatc
//
LOCUS       DATMED010000111        10229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1619909, whole genome shotgun sequence.
ACCESSION   DATMED010000111 DATMED010000000
VERSION     DATMED010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619909"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1264)
                     /locus_tag="VNP73_02785"
     CDS             complement(158..1264)
                     /locus_tag="VNP73_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL64876.1"
                     /translation="MLTVEKLSKSFADGQNQIVAVNDVSFEVPDGKLFTLLGPSGCGK
                     TTTLRCIAGLESPERGLIKVDDRVFFSSKTGKRIKPNERGLGMLFQSYAIWPHMTVYD
                     NVAFPLVVERRGQRRSKAAIRERVEKVLTTVQLQRVADRPATDLSGGQQQRVALARAL
                     VLEPPLLLLDEPLSNLDAKLRGDMRLELKRLQREIGITTVYVTHDQAEALAMSNLVAV
                     MSEGAIQQVGSPRDIYSRPASRFVADFIGSSNFIEGSIEQRDPDGCYVVRTAAGLLRA
                     SSTLDYSVGDTAVVSIRPEHVELKLGAPPSTEPNVWGGIVVMRGFLGEAFDHLVAVGP
                     VQVRARTNPAGSVSRDEKVTILLPSDACSLVPGD"
     gene            complement(1266..2948)
                     /locus_tag="VNP73_02790"
     CDS             complement(1266..2948)
                     /locus_tag="VNP73_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWL64877.1"
                     /translation="MLLVVGAVLLIVGYLALTPLGYLLWGTFFDEGGFTLRFFEEALS
                     QQDFAEVALTSFAFSGGATVLAMVIGCALAYLLVRTDIALKRVIFAGSLVPLIVPSIL
                     QVISWIFLLAPRTGFLNRIAASLSLGPFDIFSLPGMIFVEGMGLAPLVFLLMAAALRS
                     MDPSLEESALMSGANLNTVIRRVTLPIVRPALFAALLIMGVHSLQAFETPAILGIPDG
                     TWTFTSRIWNSLHGFPREFGPAGAYSVMLLALTGVGVFLHSRLTKRSTAWQTVTGRGY
                     RPRPIELGRWRGPLTAAALGYVVFVVVLPLMIMFFASFQPYFAAISVAATENLSLDNY
                     RLIFERRNILRSTQNSLVLGISSATFIMFLTAVAAWLVVRTRVAGRWVVDNLTFLPIV
                     VPGLVMGVALLFVYLRHPVPIYGTLWILFIAFLTKYMPYGMRFASSSMFQIGVELEES
                     ARMSGASWWQSFRRIVLPLLFPGLIAGWIYVLVVSIRELSTAILVYSPGNEVLAVEIW
                     ELWQDGRLTQLAALGTLMVVALLALAGLAYKIGSRIGIRETALEPAPLPGEL"
     gene            complement(2959..4065)
                     /locus_tag="VNP73_02795"
     CDS             complement(2959..4065)
                     /locus_tag="VNP73_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWL64878.1"
                     /translation="MLLLATACQSPPTSDQPSDTNQLEQFRTETNDRLEEITGELEGL
                     DEKARRERLIELAQEEGATLTLYGVTNLEEMAENIDAFEEDTGMTVNYYRAGSEEILQ
                     RLVEEQKAGFRAADAVNENGAEMVFLDREGMFAPVVTPASEDLPEKGVHETWFWSYID
                     IFAPAWNPKLIPASEAPKTWQDVLGKYDQELLMEIGDIEWFATLVKHFVAEEGMTEEE
                     AIALFEDAAKNATFVDGHTLMVELVAAGEYGIAASPYSHRNAEFKEDGAPVEWEPAVA
                     PLVARPEGVAVHSTAEHPAAALLFVEWYLTDGQKLGLTQERQPASTAVPGGGLPEDYE
                     NIVVDVDAVLDEFDRWSELYDEIVQMSGEEVLVD"
     gene            complement(4104..4955)
                     /locus_tag="VNP73_02800"
     CDS             complement(4104..4955)
                     /locus_tag="VNP73_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWL64879.1"
                     /translation="MTDFDEARSKSHANWQEMAPGWQKHGDYLWNTSRHVTEWMVEKA
                     DLKPGDTVLDVAAGPGDSGFTAAKLVGNTGRVITTDFSQQMVDVAEARGASLGITNAE
                     FKQMDAEKMDLDSAGVDAVLCKWGFMLMLDPAAALRECHRVLRPGGRVALSVWGGPEQ
                     NPWITVMGMVMMQQGYTPQGDPFGPGGMFSMAQADTIRSMLSDAGFSVVEVEEAEVRW
                     RFDDFNGVWAFATELAGAISALVKQLPPEEVEDFKKAVIPAIEPYKSGDGYDLPGVTM
                     NAAATKS"
     gene            complement(4983..5657)
                     /locus_tag="VNP73_02805"
     CDS             complement(4983..5657)
                     /locus_tag="VNP73_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64880.1"
                     /translation="MFQEDAPLVRRKLFGKRRLGAILACTAVLAACGTAIGGLVLDTQ
                     TRGSIETPAADGSTRLSPGELQEGSPSKDDFLSRWGNFVGTGVDVVVGGVQTAVSTAR
                     DLPGRVINAIAGPTDDSIDSADLPALSQADVRGPNLTSEATRNPDRGEPEDGALRPGS
                     TRSSDPPDDTTTTQDEPSTTEEDFDDEPSPTPEEEPSESPSPDPSSPSPEPSESPSPT
                     PSPSDP"
     gene            complement(5682..8285)
                     /locus_tag="VNP73_02810"
     CDS             complement(5682..8285)
                     /locus_tag="VNP73_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HWL64881.1"
                     /translation="MTTATQDLRSTGRHAIPRTWVANLGWELPLILLLVIAAFAVHGA
                     NLFHYPAFQEDEGIYAAQAWSVLRDQRLTPYTYTYDHAPGGWIFAAFWMLISGGPEMF
                     GGSILSGRIFILVLMTACVPLLYRIARKLGCGVAGATVATLLFSLSPLAIGYQRMFLL
                     DNIMIFWVLLSINLMLDGWGRLSRLVMSGVCFGIAVLTKETAIFLIPAMLYIATKQRW
                     SHQGRFAVTGWLVPAIAVTSLYPLYAALKGELTGGPGASLIDALRWQTERGPGGELGS
                     PVWDALTNNWLPVDPLLIVGGAAAVAINLIAGLKNKLLMACGLLGLMPLIYLLRGGVV
                     FDFYIVVAIPFLALNIGTLLRSAPRLRPIALLVVAGVVAGTFVVTNSFDPDRARALVA
                     QRPGKANQEAVAWIKANVEPKSYIVARDDFWADLREEGLMGPAFPNVHSHWKVAADPA
                     VRDDIFENNWKRVDYLIMTPGLRSAFEATENEVALEALDNSDLARVWTSDSYRVELWK
                     VDRQGSGEIELLDGSYEFIADRFEQDGAFVDAEGIVTSEAQAYALLRAAWSDRPEDFR
                     RIWEWTEANLQRPDGLLSWQWQDGEIVDANSASDADTDAALALLLAGEAWDDEELLAE
                     GRVLAHAIWEGDVAEIGGTPYLTAGNWAVTDEIVMNPSYLAPYAYRFFAEVDPEHDWY
                     ALIDSSYEFLFETTSAPLDAPDSAGLPPDWIALDPATGESTVVEVNGRDATNYGYDAA
                     RTWWRVALDLQWADEGRAKAYLDQAGFLADEVERKGHVSAVYEHDGTVVEAEPSIVST
                     AGATAALAATDPEQAGALYASDIVGRAQDAAEGTYWNDETDLYAQEWGWFATALYGDA
                     LWSPWDNASSG"
     gene            complement(8282..9508)
                     /locus_tag="VNP73_02815"
     CDS             complement(8282..9508)
                     /locus_tag="VNP73_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL64882.1"
                     /translation="MQALYSTALSLYAWEDQDRQDTGSAPTSFEAATQSFTILLPARH
                     EQQVIANTIQRMAELNYPRHLVQILIVIEHTDDETIGEVNGKFEELRAAGIDNARLIT
                     FDEPPINKPHGLNVGLKESTGDIVTIFDAEDEPHPDILNVVNTVMLQEQKDVVQCGVQ
                     LMNYDDHWFSALNVLEYFFWFKSRLHYHATKGVIPLGGNTVFIRRDLMLELGGWDDSC
                     LTEDADIGLRLSARGTPIRMVYEHEYVTREETPHSVGEFIKQRTRWDQGFYQVLAKGD
                     WRRLPTRTERMLALYTLSFPLFQALMMLYLPVTVYTMFFVDMPVPVAMLATLPLYMLV
                     IQFLISILGLYEFTSAHKLKPSFFSGIRMLIVYMPFQWLLAYAALRALVRHAAGTNTW
                     EKTTHTGAHREELTAA"
     gene            complement(9926..>10229)
                     /locus_tag="VNP73_02820"
     CDS             complement(9926..>10229)
                     /locus_tag="VNP73_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64883.1"
                     /translation="VSRVFEFHEDVCSLPEGAELLFRGDDVPVQAFRIGERAYGVQFH
                     FEVDTNVIATWCDYTPDLADTWGATKKELLEQAEDLLVAQQGAARAAMRNFLTLAR"
BASE COUNT         1932 a         3258 c         3153 g         1886 t
ORIGIN      
        1 gggccgctct tcggatcgag tccgtggtcg aaacatcggg tactcgggtc gtgaaagcga
       61 tacgccaccg catgaccggt gtgaaggccg ccaagtttcg gcgcgcggtg gccggcttta
      121 tccaggctcc gatcgccaag ggcgagtagg agtcctccta atcaccgggg acgagtgagc
      181 acgcatcgga aggtaacagg atcgttacct tttcatcccg cgacaccgat ccggcggggt
      241 tcgttcgtgc tcgaacctgc accggcccga ccgccacgag atggtcgaac gcttccccaa
      301 ggaacccgcg catcaccacg atcccgcccc acacgttggg ttctgtcgat ggaggggcgc
      361 cgagcttcaa ctcgacatgc tcgggtcgga tcgaaaccac cgctgtatcg cccaccgagt
      421 agtcgagggt cgatgacgcg cgcagcaagc ccgcagccgt gcgaaccacg tagcaaccgt
      481 cgggatctct ttgttcgatg gatccttcga tgaagttcga acttccgatg aagtccgcga
      541 cgaaacgtga cgcaggccgg ctgtagatgt cgcgcggcga accaacctgc tggatcgcgc
      601 cttcgctcat gaccgcgacg aggttggaca tcgcgagggc ttcggcctga tcgtgggtga
      661 cgtagacggt cgtgataccg atctctcttt gcagtcgttt gagttccaac cgcatatccc
      721 cacgcaactt tgcgtcgagg ttagacaaag gctcgtcgag cagcaggagg ggaggttcca
      781 gaaccagagc tcgtgccaac gcaacgcgct gttgttgccc gccggaaagg tccgtggcgg
      841 ggcgatcggc cacgcgctga agctgcaccg tagtgaggac cttctcgacc ctctccctta
      901 tcgccgcttt cgaacggcgc tgcccgcgtc gttctacgac caaaggaaag gcgacgttgt
      961 cgtacacggt catgtgaggc cagatcgcgt aggactggaa caacatcccg agcccgcgtt
     1021 cgttgggctt gatcctcttc ccggtcttgg acgagaagaa cacccggtcg tcgaccttga
     1081 tcaggccgcg ctccgggctc tccagacctg cgatgcagcg cagtgtcgtc gtcttgccgc
     1141 aaccggacgg tccgagcaac gtgaataact tcccgtcggg aacctcgaac gacacatcgt
     1201 tgaccgcgac gatctggttc tgcccgtcgg cgaaggactt cgacagcttc tcgaccgtca
     1261 acacgtcaga gttctcccgg aagcggggcg ggttccagcg cggtctctcg gataccgatc
     1321 cggcttccga tcttgtacgc gagtcctgca agtgccagga gcgcgacgac catcagagtg
     1381 cccaacgcgg ccagctgagt gaggcggccg tcctgccaca gctcccagat ctcgacggcc
     1441 agcacttcgt tgccggggga gtagacgagg atcgcggtgg acagctcccg gatcgacacc
     1501 acgagcacat agatccatcc cgctatcaaa ccgggaaaga gcaacggcaa cacgatcctt
     1561 cggaacgact gccaccacga tgcaccactc atccgtgcgc tttcctccaa ctcgaccccg
     1621 atctggaaca tggagctgct tgcgaacctc atcccgtacg gcatgtactt cgtgaggaag
     1681 gcgatgaaca ggatccacag cgtcccgtag atcggcacag gatgtctgag gtaaacgaac
     1741 agtagggcaa cccccatgac gagacccggc accacgatcg gaaggaacgt caggttgtcc
     1801 accacccacc tacccgcgac gcgggtacgc acgacgagcc acgcggccac ggccgtaagg
     1861 aacatgatga acgtcgcgga tgagataccg agcaccaagg agttctgggt ggagcgcagg
     1921 atgtttctgc gttcgaagat cagccggtag ttgtccaacg acaggttctc ggtcgctgcg
     1981 acacttattg cggcgaaata gggctggaag gatgcgaaga acatgatcat cagagggagc
     2041 accacgacga acacgacgta gcccaacgcg gccgcggtta gggggccgcg ccagcgacca
     2101 agctcgatcg gccgcgggcg gtatccacgg cctgtgaccg tctgccacgc ggtgctccgt
     2161 ttcgtaagtc tggagtgaag aaacacacct acgccggtca acgcaaggag catcaccgag
     2221 taggcccccg ccggaccgaa ctcgcgcggg aagccgtgga gcgagttcca gatcctcgag
     2281 gtgaacgtcc acgtcccatc ggggatgccg aggatcgccg gcgtttcgaa cgcttgcagg
     2341 ctgtgcactc ccattatcaa gagggccgcg aacagggccg gtctcacgat cggcagcgtt
     2401 actcgccgga tcaccgtgtt caggtttgcg ccgctcatga gtgccgattc ctccaatgag
     2461 ggatccatcg atcgcaacgc cgcggccatc aaaaggaaga caaggggagc gaggcccatc
     2521 ccctcgacga agatcatgcc ggggagactg aagatgtcga acggacccag tgacagcgag
     2581 gctgcgatcc ggttcaggaa tcccgtccgt ggcgccaaaa ggaatatcca cgagatcacc
     2641 tgcaggatgc tcgggacgat cagcggcacg agcgatccgg cgaagatcac ccgcttcaac
     2701 gcgatatcgg tacgaaccaa cagataggcg agcgcgcaac cgatcaccat ggcaaggacc
     2761 gtggccccac cggagaacgc gaacgaggta agggccactt ccgcgaagtc ctgttgggag
     2821 agggcctcct cgaagaaccg aagtgtgaag cctccttcgt cgaagaaggt tccccacaat
     2881 aggtagccga gcggagtcag cgcgaggtag ccgacgatca gcaatacggc cccaacgact
     2941 aggagcatct gactgccgtt agtccactag aacttcttcg cccgacatct gcacgatctc
     3001 gtcgtagagc tccgaccacc tgtcgaactc gtctaggacc gcgtcgacgt cgacgacgat
     3061 gttctcgtaa tcctcgggca accccccgcc cggcaccgcg gtgcttgcgg gctgccgctc
     3121 ttgggtcagc cctagcttct gaccgtcggt caggtaccac tccacgaaca acagagcggc
     3181 cgcggggtgt tccgcggtcg aatgcaccgc gactccttca gggcgtgcga ccaatggagc
     3241 gacggctggc tcccactcga cgggagcccc gtcctccttg aattctgcat tccggtgcga
     3301 gtaaggcgag gccgcgatcc cgtactcgcc ggcggcgacg agttcgacca tcaacgtgtg
     3361 tccatcgacg aaggtcgcgt tcttggcggc gtcttcgaac agagcgatgg cctcttcttc
     3421 ggtcatcccc tcctcggcaa cgaagtgctt gacgagcgtc gcgaaccatt cgatgtcgcc
     3481 gatctccatc agcaattcct ggtcgtattt gccgaggacg tcctgccacg tcttgggggc
     3541 ctcgctcgcc gggatcaatt tcgggttcca tgcgggggcg aagatgtcta tgtacgacca
     3601 gaaccacgtc tcgtgcacac ccttttcggg gaggtcctcc gaggcggggg taactacggg
     3661 agcgaacatg ccttcgcgat caaggaagac catctcggcg ccgttctcgt tgactgcgtc
     3721 ggccgcacgg aaaccggcct tctgctcctc gacaaggcgt tgcaatatct cttcggaacc
     3781 ggcccggtag tagttcacgg tcataccggt gtcttcctcg aaagcatcga tgttctccgc
     3841 catctcctcg aggttcgtta cgccgtagag ggtcagcgtt gcgccttctt cctgagcgag
     3901 ttcgatcagc cgctcacggc gtgccttttc gtccaggccc tcgagctcgc cggtgatctc
     3961 ctcgaggcga tcgttggttt ccgttcggaa ctgttcgagc tggttcgtgt cggatggttg
     4021 gtccgaggtg gggggtgact gacaagccgt cgcgagtaac agcacagcaa aggaaagaat
     4081 cgatagccgg cgcaaattgc ctcctatgac ttggtggccg cagcgttcat cgtcacgccg
     4141 gggaggtcgt acccgtcacc cgacttgtac ggctctatgg caggaatcac ggcttttttg
     4201 aagtcctcga cttcttctgg aggcagttgt ttgacgagag ccgagatcgc acccgcgagc
     4261 tcggtggcga aggcccacac cccgttgaag tcgtcgaagc gccagcgaac ctcagcctct
     4321 tccacctcga cgacggagaa acctgcgtcc gagagcatcg atcggatcgt gtcggcctgc
     4381 gccatcgaaa acatcccccc gggtccgaac gggtcaccct gtggggtgta accctgctgc
     4441 atcatcacca tgcccatgac ggtgatccag gggttctgct ccgggcctcc ccacacagac
     4501 agcgccactc gtccgccggg tcgcagcacg cggtgacatt cgcgtagagc ggccgccgga
     4561 tcgagcatca acatgaagcc ccacttgcag agcaccgcgt cgacgccggc cgagtcgagg
     4621 tccatcttct cggcatccat ctgcttgaac tcggcgttgg tgatcccgag ggacgcgccc
     4681 cgcgcttcgg caacgtcgac catctgctgg gagaagtcgg tcgtgatcac tcttcccgtg
     4741 ttgccgacga gcttggcggc ggtgaagccg ctgtctcccg ggcccgcggc gacatccagc
     4801 accgtgtcgc ccggcttcag atcggccttt tcgaccatcc attccgtcac gtggcgagag
     4861 gtgttccaca gatagtcgcc gtgcttctgc cagccggggg ccatctcttg ccagttggcg
     4921 tggctcttgc tccgtgcttc gtcgaagtcg gtcacggagc gcaccctagc gggcgcgtga
     4981 atctaggggt cggaagggct tggggtgggc gagggggact cgctcggttc cggcgatgga
     5041 gaactcggat cggggctcgg cgattcagac ggctcctcct ccggcgtagg ggagggctcg
     5101 tcgtcgaagt cctcttccgt tgtcgagggc tcgtcctgtg tcgtggttgt gtcgtccggc
     5161 ggatccgagc tgcgcgtcga ccccggacgc agcgcaccgt cttccggctc gccccgatcg
     5221 gggttcctgg tcgcctccga ggtcaggttc ggccccctca cgtccgcttg cgacagcgcg
     5281 gggagatcgg ccgaatcgat cgagtcatcc gtcggtccgg cgatcgcgtt gatgacgcga
     5341 cccggaagat cccgtgccgt gctaaccgcc gtttggaccc ctccgacgac tacgtcgacg
     5401 ccggtgccga cgaagtttcc ccagcgcgag aggaagtcgt ccttcgaggg cgagccctct
     5461 tgaagctctc ccggcgacaa gcgggtcgag ccatccgcag ccggcgtttc gatggagccg
     5521 cgcgtctgag tgtccagaac gagcccaccg atcgcggtgc cgcatgcagc cagcaccgcg
     5581 gtgcaggcaa ggatggctcc caaacggcgc ttgccgaaaa gcttccgccg tacgagcggc
     5641 gcgtcttctt gaaacaaagg tccactggtc atatcgatta cttacccact cgaggcgtta
     5701 tcccacgggc tccataaagc gtctccgtaa agggctgttg caaaccaacc ccactcctgt
     5761 gcgtacaggt cggtctcatc gttccagtac gtcccttcgg cggcatcctg cgcgcggccg
     5821 acgatgtcgc tcgcgtacag ggcacctgct tgctctggat ccgttgctgc cagcgcggcg
     5881 gtagctcccg cggtcgagac gatgctgggc tctgcctcga cgacggtgcc gtcgtgctcg
     5941 taaaccgcgc tgacgtgacc tttgcgctca acctcgtcgg cgaggaaccc ggcttggtcg
     6001 aggtaagcct tcgcccggcc ctcatccgcc cactgcagat cgagcgccac cctccaccac
     6061 gtgcgcgccg cgtcgtatcc gtagttcgtc gcgtcgcgtc cgttgacctc gacgacggtg
     6121 gactcgccgg tcgcggggtc gagcgcgatc caatccggtg gcagccccgc cgagtccggc
     6181 gcgtccaaag gcgcactcgt cgtctcgaac aggaattcgt aggaagagtc gatgagcgcg
     6241 taccaatcgt gctccgggtc gacctcggcg aagaaccggt atgcataggg cgcgaggtaa
     6301 ctcgggttca tcacgatctc gtcggtgacc gcccagttgc cggcggtcag gtacggggtg
     6361 ccgccgatct cggctacatc accttcccag atcgcgtgcg cgaggacccg gccttcggcc
     6421 agcaactcct cgtcgtccca cgcctctccg gcgagcagaa gagcgagcgc cgcgtcggtg
     6481 tcggcatcgg acgcactgtt ggcgtcgacg atctcaccgt cctgccattg ccacgacagc
     6541 aatccatccg gccgttggag atttgcctcc gtccattccc agatgcgacg gaagtcctcg
     6601 ggtcgatcgg accacgccgc gcggaggagt gcgtaggcct gcgcctccga tgtcacgatg
     6661 ccctccgcgt ccacgaacgc tccgtcctgc tcgaagcgat ccgcgatgaa ctcatacgag
     6721 ccgtcgagta actcgatctc gccggaaccc tgcctgtcga ccttccacag ttcgacccgg
     6781 tacgagtcgc tcgtccacac ccgggcgaga tccgagttgt ccagagcctc gagggcaacc
     6841 tcgttctctg ttgcttcgaa agcagatctc aatcccggcg tcatgatcag gtagtcgacg
     6901 cgtttccagt tgttctcgaa gatgtcgtcc cggacggcgg ggtcggcggc gaccttccag
     6961 tgactgtgga cgttcgggaa ggcgggtccc atcaggcctt cctcgcgcag gtcggcccag
     7021 aagtcgtccc tggcgacgat gtagctcttt ggttcgacgt tggccttgat ccaagccacc
     7081 gcttcctggt tcgccttgcc cggccgctgt gcgaccaggg cgcgcgctcg atccggatcg
     7141 aaggagttcg tcacgacgaa cgtccccgcg acgacgccgg ctaccacgag caaagcgatc
     7201 ggccgcaacc gcggtgcaga gcgcagcagc gtgccgatgt tcagcgccag gaacgggatc
     7261 gcaaccacga tgtagaagtc gaatacgact ccgcctcgaa gcagatagat cagcggcatc
     7321 aggccgagta gcccgcacgc catcaagagc ttgttcttca gcccggcgat cagattgatc
     7381 gcgaccgccg ccgcgcctcc gacgatcagt agtggatcga caggcagcca gttgtttgtc
     7441 agcgcgtccc agaccgggct cccgagctcc ccgcccggcc ccctctcggt ctgccacctc
     7501 agcgcgtcga tcaacgatgc ccctgggcca cccgtcaatt cacccttgag cgcggcatag
     7561 agcggataca gactcgtcac cgcgatcgca ggaaccagcc agcccgtgac ggcgaagcgt
     7621 ccctggtggc tccatctctg cttcgtcgcg atgtagagca tggcgggtat caagaagatg
     7681 gccgtctctt tcgtcaacac cgcgataccg aaacagactc cactcatgac caaacggctg
     7741 agccgtcccc atccgtcgag catcaggttg atgcttagca acacccagaa gatcatgatg
     7801 ttgtcgagca ggaacatccg ctgataaccg atggcaagag gcgacaagct gaatagaagg
     7861 gtggcaaccg tcgcaccggc caccccgcag ccgagtttgc gtgcgatccg atatagcaac
     7921 ggaacgcacg cggtcatgag aaccaggatg aagatgcgtc cgctgaggat gctgcccccg
     7981 aacatctccg ggccgcccga gatcaacatc cagaacgcag cgaaaatcca accacccggc
     8041 gcgtggtcgt aggtgtacgt gtagggggtg aggcgttggt cgcgcaaaac cgaccacgcc
     8101 tgcgccgcgt agatgccctc gtcctcctgg aaggcgggat agtggaagag gttcgctccg
     8161 tgcacggcga atgcggcgat cacgagcagg aggatcagcg gcaactccca accgaggttc
     8221 gcaacccacg tgcgcgggat ggcgtgccgg ccggtggacc taaggtcttg ggttgcggtg
     8281 gtcaagccgc cgtcagctct tctctatgcg caccggtgtg cgtcgtcttt tcccacgtgt
     8341 tggtgccggc cgcatgtcgt acgagggcac gcagcgcggc ataggcaagc agccactgaa
     8401 acggcatgta cacgatcagc atcctgatcc cggaaaagaa cgacggcttc agtttgtgcg
     8461 cggacgtgaa ctcgtagaga cccaggatgc tgatcaggaa ctggatgacg agcatgtaca
     8521 gcgggagcgt cgccagcatc gcgaccggga ccggcatgtc gacgaagaac atcgtgtaga
     8581 ccgtgaccgg caggtacagc atcatcaggg cttggaacaa cgggaacgac agcgtgtaga
     8641 gggccagcat ccgttcggtc ctcgtcggga gcctccgcca gtcgcctttg gccagcactt
     8701 gatagaagcc ttgatcccaa cgcgtgcgct gtttgatgaa ctcgccgacc gagtgcggag
     8761 tttcttcccg ggtcacgtac tcgtgttcgt agaccatccg gatcggggtc ccgcgggcgc
     8821 tgaggcgcaa gccgatgtcg gcgtcctcgg tcagacaaga gtcgtcccac ccgccgagct
     8881 cgagcatcag gtcccttctg atgaagaccg tgttgccgcc caaggggatg acgcctttcg
     8941 tagcgtggta gtgcaagcgg ctcttgaacc agaagaagta ctcgagcacg ttgagagcgg
     9001 agaaccagtg gtcgtcgtag ttcatcagtt gcacgccgca ctggacgacg tccttctgct
     9061 cttgcaacat gacggtgttg accacgttca ggatgtcggg gtgcggctcg tcttcggcat
     9121 cgaagatcgt cacgatgtcg cccgtcgatt ccttgagtcc cacgttcagc ccgtgaggct
     9181 tgttgatcgg gggttcgtcg aacgtgatca gtctggcgtt gtcgatgccg gcggctctca
     9241 gctcttcgaa cttgccgttg acctctccga tcgtttcgtc gtcggtgtgt tcgatcacga
     9301 tgaggatctg gacgaggtgg cgcgggtagt tcagctcggc catccgctgg atcgtgttcg
     9361 cgatcacctg ctgctcgtga cgagcgggga gcaggatcgt gaacgactga gtggccgcct
     9421 cgaacgatgt cggcgcgcta ccggtgtcct gtcggtcttg gtcctcccac gcgtacaggc
     9481 tgagcgcagt gctgtagagg gcctgcaccg tgagtagcac ggaaaagccc gcgagaaacc
     9541 aagccccaag ttccaaatgc ttcgctaata cccagttatc gccagtcgca ctccttacgg
     9601 ccgtggcgag cgaagagttc ctcccgcaga accagccctc ggcccgttaa taggaggcca
     9661 cggctttgaa cccggcgagc cagcccgcgc aaagccaggt cgtcgagtgc ggcgcggacc
     9721 tcgaccgcgc tgaagcccgc agtcgctcga ccgacaacga tgcgccctcg gcttacgcca
     9781 tcaaggagaa gagactgctg aagtggttga caggttgcag cgtcgctacc ggacggcgat
     9841 gccggcagac atgtggggtg gtgctgggag gcacttgcag aatcttccga attcggaaga
     9901 tttcagaggg ggtgtgagcc gatggtcacc gtgcgagcgt gaggaagttc cgcatcgccg
     9961 cgcgtgcggc cccctgctgg gcgaccaaca gatcttccgc ttgctcgagg agctccttct
    10021 tggtcgctcc ccatgtgtcg gctaggtcgg gcgtgtagtc gcaccacgtc gcgatcacgt
    10081 tcgtgtccac ctcgaagtgg aactgcacgc cgtaggcacg ttcgccgatc cggaatgctt
    10141 ggaccggtac gtcgtcgccg cggaacagaa gctcggcccc ctcgggcagg gaacacacat
    10201 cctcgtggaa ctcgaagacc cgcgacacc
//
LOCUS       DATMED010000112         9126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1622823, whole genome shotgun sequence.
ACCESSION   DATMED010000112 DATMED010000000
VERSION     DATMED010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622823"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VNP73_02825"
     CDS             <1..1050
                     /locus_tag="VNP73_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64884.1"
                     /translation="QYPAFARGANPIEIFTGVSILALGILRVPVTLGDLELIALPLGA
                     LIAVGVGIAWATRAAFTPTAARDSAATAAQIALVFAVIAFLLSVIFRIGGAEPVRASA
                     ISSVVWAFLWAWLFSYLALARGGRSWRQVVAATSERLATRSRTLAAGIGGGAVMLTTA
                     FLLGAAAVLLWLIVVLASGELHKSFSIGDALAAVLYLAAFLPNVVIAVIALSLGAPVL
                     RGAQVGIGGNAIGRIQEVSLLDWPGSMPWYAFLLLAIPIVTSLVGGMWQGRAHPQAPM
                     WRSLLMAATIFSVGLALLGWLGETRIGAGLAGHKGLARVAVSPGWTFVAAMIWSTVGG
                     WLGWELGHRWGGSRA"
     gene            1043..1705
                     /locus_tag="VNP73_02830"
     CDS             1043..1705
                     /locus_tag="VNP73_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL64885.1"
                     /translation="MLEVFAHYVVGPLACNCYIVGDRAAKEAIVIDPGDDAEDLAAAI
                     AERELTVKAIVATHAHFDHVVGAGRLRELTGAPFYLHADDGPMLDWMQESGRLFLGLE
                     LPPPPEVDRSPTEGDVLAAGTARLEVLHTPGHSPGSISLVSDQAIFSGDTLFAGSVGR
                     TDLPGGDTDQLIEAIKKKLFTFEDDLPVYPGHGPATTLGEERKFNPFVGEGGAFWAPG
                     NA"
     gene            complement(1702..2166)
                     /locus_tag="VNP73_02835"
     CDS             complement(1702..2166)
                     /locus_tag="VNP73_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWL64886.1"
                     /translation="MPACKLTIVALTAAVALVAPACAVDVEDDDDDVIQEVELEAYDF
                     YFEETSLPLQLGGDVIVDFVNAGDATHSFTVPDLDLEVEAQSGESTEVEFSLPDEPGV
                     LDFFCKFHPDDMNGTISIGGAEAPLEEEGAVEDEDVDEDTAEDAPGGPGYNY"
     gene            complement(2183..2425)
                     /locus_tag="VNP73_02840"
     CDS             complement(2183..2425)
                     /locus_tag="VNP73_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64887.1"
                     /translation="MAEGKQKKVTQKFTMDLYLTAPDGRVRKAYAGELRRLQRLGFRE
                     TDRKLGSREDHVRVTLEREIDKYPGVPLPAVPPYLT"
     gene            complement(2452..3417)
                     /locus_tag="VNP73_02845"
     CDS             complement(2452..3417)
                     /locus_tag="VNP73_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HWL64888.1"
                     /translation="MLMAVCHSSKTGWTRVEDLDSLSDLRVQAGNVLWAEADVSTLTE
                     KDVTMIAGEFGLHPLAVEDAVHTRQRPKIEPYGDHLFAVFHQVDEEANQFEARQIACF
                     IGDRYLLVLHENADRLLEIAKKRFREQGQVLESPALLLHTMLDTVVDDYQLKADTLED
                     QVEELEEIVLETPTAPVQRQLYSLKQRVSRLRRYVLPATRLLEWMMGPADKPFPIGDT
                     KLFRDIHDHLLRMADQIRNIDELDQAVLDLVRNEHAQSLNEIQKRLAAWAAIFGVGTL
                     IAGVYGMNFELVPKDQTLVGFWFAVALMALASIGLYIYFKRRDWL"
     gene            3452..4882
                     /locus_tag="VNP73_02850"
     CDS             3452..4882
                     /locus_tag="VNP73_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWL64889.1"
                     /translation="MTDTKGYLVAGQWKQGSDSFEVPNPYDGSVVAKVASPTDGDVEA
                     AVSAAAESFQETRSLPVHARAEALMQISNRLKERGEEVADLISKEGGKPIKWASVEAA
                     RAVSTFRWAAEICRSYGGEVMRLDTEESLGSRMALIRRFPIGPVLAITPFNFPVNLVA
                     HKMAPALAVGAPIVIKPATKTPLGALLLGELFAETDLPAGMLSVIPIGGSQAGELAKD
                     ERFKKISFTGSSQVGWDLKASDPKKAVTLELGGNAGVIVHSDGDLDKAAQRVAFGGYY
                     QAGQSCISVQRVLAQADIFEDFKQKLIVEVEKLKTGDPSDPETDVGPLINRDSLDKVS
                     GWVDEAVQGGAEILTGGKREDPFYLPTVLSRTRSEMKVRQEEIFGPVVTLESYGDFAE
                     AIELANETRYGLQAGVFTRDIERILLAHRDIEAGGIIVNDVASFRADQMPYGGVKDSG
                     SGREGLRYAMDEMTEIKVLVLSDAIL"
     gene            4932..5252
                     /locus_tag="VNP73_02855"
     CDS             4932..5252
                     /locus_tag="VNP73_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64890.1"
                     /translation="MIGLVFAPFFSRRRVEDGVLLCEGAAWPRRLGWHYRAVTFGHVV
                     LCVDELDEGVLAHELVHVRQYEKWGPLFIPAYLVAAGMARVRGGHAHRDNRFEVNARE
                     RSGH"
     gene            5379..7244
                     /locus_tag="VNP73_02860"
     CDS             5379..7244
                     /locus_tag="VNP73_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64891.1"
                     /translation="MRGTRSAVSIVAVSVLVGALLAAPATASHTTGDCPTPVPPEDVQ
                     RGLTGEGATVAAGRTLEHFDAEVLGVLPDGLGPGRDMILVELAGQVLDDNGGVWAGIS
                     GSPIFVGDRLLGSVSFALAFGPAHVAGVTPATEMYEVMEYPTETDTSARMPMRVPISS
                     SLRKRIALATDTEVSEVASAFRPIKMPVSVSGAPGRVIDSVTQVIARDRLPLIPYAGG
                     AASSGVQAAQSSPLRPGDNIVAAMSYGDVTFAGIGTATFVCEGRVMAFGHPFLWEGAT
                     ALGANAANSLLIVQDPFFSFEMATVEEGLGTIDQDRFAGIRGMFGTSPATMPITSDVN
                     AVNTARSRSGRTDVVSSEMFPFLAFIHLSGNIVMTMDQYSEGSADVSWTVTGRTESGQ
                     EWSLTRDNMFSSEFAIPDDASFELSSALSTLYYNQFEPIEFTGLDITATADDDRKQYT
                     ISKVLVSDTGRNYRDKRRIKVAPGSSVFLRVVLKPHGPGDEQNVDLAVEIPNRARSSG
                     FIEIRGGALSFEEQEICFYDPTACAQVGGKKIESFDDLLASLAEQPKNNELLARLRMG
                     GRGRVKSKASQTLDKVIAGEAVVFVRIPGGCCGGGGRGGRGGAIEPDPGGVPPTE"
     gene            complement(7248..8201)
                     /locus_tag="VNP73_02865"
     CDS             complement(7248..8201)
                     /locus_tag="VNP73_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWL64892.1"
                     /translation="MDPLDCAPRVRLGRWPTPTRRLDNVSELLGREVWIKSEEECGTW
                     GGNKVRKLEYILAAAEAHGARKLVTYGAGTSSWAAALVFHAVPRGFEVVLGLGGGIPD
                     PNRRLYEKTSTSVFEMSSYNFTPVAAVGAMARAGMLTATRLPAGGSGLPGDLGSMGAG
                     IEIGAALSSGELPVPGAIYCPAGTSGTAAGLAVGVAWGGHQVPIRAVRVTPIPLGTAA
                     MITWHARRLAAFVAPRTGRSVRPAPVASDGRFFPPAYGASNAASIEAIEIARRDSLEL
                     DPTYAAKGFAALIDAARRSPGGPLLFLHTSPGPLPGPGETY"
     gene            8248..>9126
                     /locus_tag="VNP73_02870"
     CDS             8248..>9126
                     /locus_tag="VNP73_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HWL64893.1"
                     /translation="MVAADTQDLHSLAQVDPDEILDGLNESITRRMPSYRDLYRRWER
                     LQWRVSDLDFTQDRADWLALDDHHKERLLWTLASFFVAEQRVADTLSPFVMAAPKLDQ
                     RIFLATQCVDETRHAIFFDRWFDEAAGLPQDDFKARIVESRRWVGPGFEPLFDEYLEE
                     VTSELRRRPTDLALFAKAVAMYHIVIEGVLALTGQKFILAWARNNQVLPGFRAGFTAV
                     ARDESRHVAFGARVLRDLLDTDPSLIEPIHEGIRETLRLGTRLYQPPNGDVTYTSAFG
                     YSLGDLFNYGGMQLEKK"
BASE COUNT         1560 a         2841 c         2990 g         1735 t
ORIGIN      
        1 cagtacccgg cattcgctcg cggggcgaac ccgatcgaga tcttcacggg cgtgtcgatc
       61 ctcgctctgg ggatcctgcg cgtcccagtc acgctcgggg atctggaact cattgcactg
      121 cccctgggcg cgctgattgc ggtgggcgtg ggcatcgcat gggcgacccg ggcggctttc
      181 acacccaccg ctgcgcgcga tagcgcagcg acggcagccc agatcgccct ggtattcgcc
      241 gtgatcgcgt tcttgctgtc ggtcatcttc aggatcgggg gtgcagagcc ggtgcgcgcg
      301 agcgcgatct cgtcggtcgt gtgggcgttc ttgtgggcat ggctgttctc atacctcgcg
      361 ctggctcggg gaggtagatc gtggcgccaa gtggtagctg cgacctccga acgtctcgcg
      421 acgcgctcgc ggacgcttgc cgccggaata ggtggtgggg ctgtgatgct cacgaccgcc
      481 ttcctcctcg gtgcggccgc cgtcttgttg tggttgatcg tggtgctcgc gagcggcgag
      541 ctccacaagt cgttctcgat cggcgacgcg ctcgcggccg ttctctatct tgcggcgttc
      601 cttcccaacg tcgtgatcgc ggtgatcgcc ctatccctcg gagcacccgt cctccgagga
      661 gcgcaggtcg gcataggtgg caacgcgatc ggacggatcc aggaggtctc gctcctggat
      721 tggccgggct cgatgccctg gtacgcattc cttctcttgg cgatcccgat cgtgacgtcc
      781 ctcgtggggg gaatgtggca gggccgcgct catccgcagg ccccgatgtg gcgctcgctg
      841 ttgatggctg caacgatctt ctcggtgggc ctcgccctcc tcggctggtt gggcgagacg
      901 aggatcggcg caggcctggc gggccacaag ggtcttgccc gcgtcgcggt atcgccggga
      961 tggaccttcg ttgccgcgat gatctggtcc accgtcggag ggtggctggg ttgggagctc
     1021 ggccaccgtt ggggaggcag tcgtgcttga ggtattcgcc cactacgtgg tcggccccct
     1081 cgcgtgtaac tgctacatcg tcggcgaccg cgctgcgaag gaggcgatcg tcatcgatcc
     1141 cggcgacgat gccgaggacc tggctgcagc gatcgccgaa cgcgaactga ccgtgaaagc
     1201 gatcgtcgct actcacgctc atttcgatca cgttgtgggg gccgggcggc tgcgcgagct
     1261 gactggagcc ccgttctacc tgcatgccga cgacggtccg atgctcgact ggatgcagga
     1321 gtccggccgc ctcttcctcg gactcgagct acctcctccg ccggaggtcg atcggagtcc
     1381 gaccgaaggc gacgttctgg ccgcgggaac cgctcgtttg gaggtgcttc acacccccgg
     1441 gcactcaccg gggtcgatct cactggtctc ggaccaggcg atcttctccg gcgacacgct
     1501 gttcgccggt tcggtgggcc ggaccgacct gcccggaggg gacacggacc agttgatcga
     1561 agcgatcaag aagaagctgt tcacgttcga agacgacctg ccggtctatc cgggacacgg
     1621 gccggcaacc acgctgggcg aagagcggaa gttcaatccg ttcgtgggag agggcggcgc
     1681 gttctgggcg ccgggtaacg cttagtagtt gtacccgggt cctccgggag catcctcggc
     1741 cgtgtcctcg tcgacatctt cgtcctcgac cgcgccttct tcttcgaggg gagcttcggc
     1801 tccgccgatc gagatcgttc cgttcatgtc atcggggtgg aacttgcaga agaagtcgag
     1861 aacgccgggt tcatcgggca gcgagaactc cacctcggtg ctctcgccgc tttgggcctc
     1921 gacctcgagg tcgaggtcgg gaacggtgaa cgagtgtgtg gcgtcgcctg cgttgacgaa
     1981 atcgacgatc acatctccgc cgagttgcag cggcaaagag gtctcttcga agtagaagtc
     2041 gtacgcctcg agctcgacct cctggatgac gtcatcgtcg tcgtcctcta cgtccacggc
     2101 gcaagcaggc gcaacgaggg caaccgcggc cgtcaaagcc acgatcgtga gcttgcatgc
     2161 cggcatgcgc accgcccggt ctctaggtga ggtagggggg gacggccgga agaggtacgc
     2221 cggggtattt gtcgatctcg cgctcgaggg tcacgcgaac gtggtcctca cgcgagccca
     2281 gtttgcgatc cgtctccctg aaccccagtc tctggagtcg tcgaagctcg cctgcgtagg
     2341 ccttgcgtac ccgcccgtcg ggagccgtga ggtagagatc catcgtgaac ttctgtgtga
     2401 ctttcttctg tttgccttca gccatagggg cagatgctag cagcgtcggg gctatagcca
     2461 gtctcgtcgc ttgaagtaga tgtacaggcc gatagaggcg agtgccatca acgcaaccgc
     2521 gaaccagaat ccgacgagcg tctgatcttt cggaacgagc tcgaagttca tcccgtagac
     2581 ccccgcgatg agcgtgccga ctccgaagat cgccgcccac gccgcgagcc gcttctgtat
     2641 ctcgttcagc gattgcgcgt gctcgttgcg aacaaggtcg aggaccgctt gatccagttc
     2701 gtcgatgttc cttatctggt cggccatcct cagcaggtgg tcgtggatgt ccctgaacaa
     2761 cttcgtgtct ccgatcggga acggcttgtc ggccggtccc atcatccact cgagcaaccg
     2821 ggtcgccgga agcacgtagc gtcgtagacg agagaccctt tgcttcagcg agtagagctg
     2881 gcgctgcacc ggtgcggtcg gggtctcgag cacgatctct tccagttcct cgacctgatc
     2941 ctcgagcgtg tccgccttca gctggtagtc gtcgacgacg gtgtcgagca tcgtgtgcag
     3001 cagcagggct ggactctcca gcacttgacc ttgttcgcgg aagcgcttct tggcgatctc
     3061 aagcagccga tctgcattct cgtgcagcac gaggaggtat ctgtcgccga tgaagcacgc
     3121 gatctggcgc gcctcgaact ggttcgcttc ctcgtcaact tgatggaaca cggcgaacag
     3181 gtggtcaccg tagggctcga tcttcggacg ttgccgggtg tgcacggcat cctcgaccgc
     3241 gaggggatgg agcccgaact cccccgcgat catggtcacg tccttctcgg tgagcgtgct
     3301 cacgtcggcc tccgcccaca gaacgttgcc cgcttggacg cgtaggtcgg acaggctgtc
     3361 caggtcctcg acccgggtcc agccggtctt cgacgagtgg cagacggcca ttagcatggt
     3421 tcgaaagata gttcctaaga ggtggagggc catgacggac accaagggat acctcgtcgc
     3481 cggtcagtgg aagcagggtt cagactcctt cgaggtgccc aatccctacg acggttccgt
     3541 ggtggcgaag gtggcctcgc ccaccgatgg agacgtcgaa gcagccgtgt ccgccgccgc
     3601 cgagtccttc caggagacac gctccctgcc cgtgcacgcg cgcgctgaag cgttgatgca
     3661 aatctccaac aggttgaagg agcgcgggga ggaagtagcc gatctcatct ccaaggaggg
     3721 cggcaaaccg atcaagtggg cctcggtcga agccgcccgt gccgtgtcga ccttccggtg
     3781 ggcggccgag atctgtcgtt catacggggg cgaggtcatg cgactcgaca ccgaagagtc
     3841 gctcggaagc cggatggctt tgatccgccg tttcccgatc ggtcctgtac tcgcgatcac
     3901 cccgttcaac ttccccgtga accttgtagc gcacaagatg gccccggcgc tggcggtcgg
     3961 cgcaccgatc gtcatcaagc cggcgaccaa aacgcctctg ggcgcgctgt tgctcggcga
     4021 gctgttcgcc gaaaccgatc taccggcagg gatgctgtcg gtgatcccta tcgggggcag
     4081 ccaagcaggc gagcttgcga aggacgagcg attcaagaag atctcgttca cgggttcgtc
     4141 gcaggtcggg tgggatctga aggcgtcgga tccgaagaag gccgtgacac tcgagctcgg
     4201 tgggaacgcg ggagtcatcg tgcactcgga tggggatcta gacaaggccg cgcaacgcgt
     4261 tgcgttcggc ggctattacc aagcggggca gtcatgcatc tcggttcagc gagtgctggc
     4321 gcaggccgac atcttcgaag atttcaaaca gaagctgatc gtcgaggtcg agaagttgaa
     4381 gaccggcgat ccctcggatc ccgagaccga cgtgggtccg ttgatcaacc gcgactcgct
     4441 agacaaggtg tcgggatggg tcgacgaagc ggtgcaaggg ggagccgaga tattgaccgg
     4501 cggcaagcgc gaggacccct tctatcttcc gacggttctt tccaggacgc gcagcgagat
     4561 gaaggtgcgt caggaggaga tcttcgggcc ggtcgtcacg ctcgagtcct atggtgactt
     4621 cgccgaagcg atcgaacttg cgaacgagac ccgctatggc ctccaagcgg gagtgttcac
     4681 acgggacatc gagcgcatcc tgctggccca ccgtgacatc gaggccggcg ggatcatcgt
     4741 caacgacgtg gcttcgttcc gggccgacca gatgccgtac gggggcgtga aggattccgg
     4801 cagcggtcgc gaaggtctgc gctacgcgat ggacgagatg acggagataa aggtcttggt
     4861 cctctccgac gcgatccttt aaaacggcag cgatccggat cctccgctac ctgtgggccg
     4921 gcctctggtc ggtgatcggt ctcgtcttcg ctccgttctt ctcacgccga cgggttgaag
     4981 acggggttct cctttgtgag ggggccgcgt ggccgcgccg tctcggctgg cactatcgag
     5041 ccgtcacttt cggccacgtc gttctgtgtg tcgacgaact cgacgaggga gtcctcgcac
     5101 acgagttggt ccacgttcgc cagtacgaga agtggggccc cctcttcatc cctgcttatc
     5161 tggtcgcagc cgggatggcg cgggtccggg gaggccatgc gcatcgggac aaccgcttcg
     5221 aggtgaacgc tcgagagcgt tccggtcact agcccgggaa accctgcaac ctttcgacct
     5281 cgggggacgt ctatgagtcg tcgcaaggag gcgaaggtcc tagttttggc ggtgccccag
     5341 cctcatactt cgtttacttg caaggaagga gaggcgtgat gaggggtacc cgctcggccg
     5401 tttccatcgt ggccgtttcc gttctggtcg gcgcgctgct tgcagctccg gcaaccgcat
     5461 ctcacaccac gggtgactgt cccacaccgg ttccgccgga agacgtgcag cgagggctga
     5521 cgggtgaggg agccaccgtc gcagcgggcc gaaccctcga gcacttcgat gccgaggtac
     5581 tcggagtcct ccccgatggc ctcggtcccg gcagggacat gatcctcgtc gagctggccg
     5641 ggcaggtgct cgacgacaac ggcggagtgt gggcaggcat atcgggttcc ccgatcttcg
     5701 tcggtgaccg gctgctcggt tcggtctctt tcgcactcgc tttcggtccc gctcacgtgg
     5761 ccggcgtcac gccggcgacc gagatgtacg aggtcatgga gtacccgacc gaaacggata
     5821 cctcggcacg catgccgatg cgcgttccga tctcatcgtc actccgcaag cgcatcgcac
     5881 tggcgaccga caccgaagtg tccgaggtag cgagtgcgtt cagacccatc aagatgccgg
     5941 tctcggtatc cggcgctccc ggtcgggtga tcgattccgt cacgcaggtg atcgctcgcg
     6001 atcgtctgcc gctgatcccc tatgcaggtg gtgcggcttc gtccggtgta caagctgccc
     6061 aatcgagtcc tctgcgcccg ggagacaaca tcgtcgcggc gatgtcttac ggcgacgtca
     6121 cgttcgcagg catcggcacc gcgaccttcg tgtgcgaggg aagggtgatg gcgttcggtc
     6181 acccgttcct gtgggagggg gcgaccgcac tcggcgcgaa cgcggccaac agccttctga
     6241 tcgtgcagga cccgttcttc tcgttcgaga tggcgaccgt cgaagagggt ctcggcacga
     6301 tcgaccaaga ccgcttcgcg ggtatcagag ggatgttcgg cacctcgccg gcgacgatgc
     6361 cgatcacttc ggacgtcaat gcggtcaaca ccgctcggtc ccgaagcggt cggaccgatg
     6421 tagtcagctc ggagatgttc ccgttccttg ctttcatcca tctgtcgggc aacatcgtca
     6481 tgacgatgga tcagtactcg gaggggtctg ccgacgtttc ctggacggtc accggcagga
     6541 ccgaatcggg gcaggagtgg tcgctgacga gagacaacat gttctcgtcc gagttcgcga
     6601 tccccgacga cgcgtcgttc gagctgtcgt ccgcactcag caccctctat tacaaccagt
     6661 tcgagccgat cgagttcacc ggtctcgata tcaccgcgac cgcagacgac gatcggaagc
     6721 agtacacgat ctcgaaggtg ctcgtctcgg atacgggtcg gaactaccgc gacaagcgac
     6781 ggatcaaggt cgcccccggc tcgagcgtgt tcctacgtgt cgtcctcaag ccacatgggc
     6841 cgggcgacga gcagaacgta gacctcgcgg tcgagatacc caaccgcgcg aggtcgagtg
     6901 gcttcatcga gatacgcggc ggagcgttga gtttcgagga gcaggagatc tgtttctacg
     6961 atccgacggc ttgtgcccag gtgggcggca agaagatcga atcgttcgac gatctcctcg
     7021 cgtcgctggc ggaacagccc aagaacaacg agctgctcgc ccgcctgcgc atggggggac
     7081 gagggcgcgt gaagagcaag gcgtcgcaga ccctggacaa ggtcatcgcc ggtgaggccg
     7141 ttgtgttcgt gcgtatcccg ggtggctgtt gtggcggcgg tggccgtggt ggccgtgggg
     7201 gagcgatcga gcccgatccg gggggcgtgc ccccaaccga gtagtcctca gtacgtctcg
     7261 cccgggccgg gcagtggtcc gggcgaggtg tgcaagaaca acagtgggcc tccgggcgac
     7321 cggcgggcgg catcgatcaa tgcggcgaac cccttggccg cgtaggtggg atcgagttcg
     7381 agcgaatcgc gccgggcgat ctcgatcgcc tcgatcgacg cggcgttgct ggctccgtac
     7441 gccgggggga agaaccggcc gtcactcgcg acgggcgcag gacgaaccga ccggcccgtc
     7501 cgcggggcaa cgaaggccgc cagtcgacgc gcgtgccacg tgatcatcgc agcagtaccg
     7561 agcgggatag gtgtgactct caccgcgcgg atggggacct gatgaccgcc ccacgcgaca
     7621 ccgacggcca ggcctgcggc ggtgccggac gttccggcag ggcaatagat cgcgcccggc
     7681 accggtagtt cgccggagga gagagccgca cctatctcga tgcccgcccc catcgagccg
     7741 agatcaccgg gcaggccgct accacccgca ggcaaccgtg tggccgtcaa catccccgct
     7801 cgggccatgg ccccgaccgc tgcgacgggc gtgaagttgt aagagctcat ctcgaacacc
     7861 gaggtgctcg tcttctcgta cagccgccgg ttcgggtctg gtatcccacc acccagcccc
     7921 agcacgacct cgaacccacg cgggaccgca tggaacacca aggctgccgc ccacgatgaa
     7981 gttcccgcgc cgtaagtaac gagcttgcgc gcgccgtgtg cctcggccgc cgcgaggatg
     8041 tactcgagct tgcgcacctt gttcccaccc cacgttccgc actcctcttc actcttgatc
     8101 cacacctcgc gcccgagaag ttcggagacg ttgtccagtc gacgagtggg cgtgggccag
     8161 cgacccagcc ggacgcgagg ggcacaatcg agcggatcca tccgggaagg ttgccctccc
     8221 gttgtcgtaa gggcaaaatc accccagatg gttgctgccg atacccaaga cctgcactcg
     8281 cttgctcagg tcgatccgga cgagatcctc gacggactga acgagtcgat cacccgcagg
     8341 atgccctctt accgagatct ctaccggcgt tgggagcggc tgcaatggcg agtctcggat
     8401 ctggatttca cccaggaccg agccgactgg ctggccctcg acgaccacca caaggagagg
     8461 ctgttgtgga ccctggcctc gttcttcgtg gcggaacaaa gggtggccga cacgttgagc
     8521 cccttcgtga tggctgctcc caaactcgac cagcgcatct tcctggctac acagtgcgta
     8581 gatgagacga ggcacgccat cttcttcgac cgctggttcg atgaggctgc cggtctgcct
     8641 caagacgatt tcaaagcccg gatcgtcgag tcgcgccggt gggtaggtcc gggtttcgag
     8701 cctctcttcg acgaatatct ggaggaggtc acttcggaac tgcgtcggag gccgacggat
     8761 cttgcgttgt tcgccaaagc tgtcgcgatg taccacatcg tgatcgaagg ggttctcgct
     8821 ctgaccggcc agaaattcat ccttgcgtgg gcccgcaaca accaggtact ccccggcttc
     8881 cgggcgggat tcaccgcggt cgcgcgcgat gaatctcgac acgtcgcctt cggagcgcgg
     8941 gtgctcagag acttgctgga caccgatcct tctttgatcg agccgatcca cgaagggata
     9001 cgggagacgc tccggctcgg cacgcgcttg tatcaacctc cgaacggaga tgtgacctat
     9061 acgagcgcgt tcggttactc gctcggagat ctcttcaact acggcggcat gcagctggag
     9121 aagaag
//
LOCUS       DATMED010000113         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3159357, whole genome shotgun sequence.
ACCESSION   DATMED010000113 DATMED010000000
VERSION     DATMED010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3159357"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1633)
                     /locus_tag="VNP73_02875"
     CDS             complement(134..1633)
                     /locus_tag="VNP73_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL64894.1"
                     /translation="MGRLTEIRWVFVVALVALIGGAACSGGPVPQASQSQSPGQILDD
                     SSGGNLGGKSSGGKLGGSKSGGGGLGASGSGGGSTGGGGIPADATDGNTVSATGETVS
                     EKEQAERAHRLGKGLPNVNLWPDSTAQMGFTEDQITLCGHAALTFAEAFDTRPEDINV
                     YWSMVNDHGGIYGRNVQLTIEDDAYRPDNAVQAAERCKAKNPFMLIGGIGFDQIPAVR
                     AWNEQEQNRLLYVHHIAREDLDKRYSFSFLPSVEQSGLLAAKWIVAKHRNDKIGILWR
                     QSEAWQPGHKTFLAAAKRAGLDIVADLPVQKDQAVYTQQIAELQSRGAETVFLWENGL
                     ADIEVIKQAKNQDYHPTWVVFPFQVMTDTLKEDMLNPPVEGIAMWPAYAPGATKGPFS
                     AYADEIKRYEAAQRQYGRAQKGNDILWMTWLAMKQVHQWLLDCGPNCDRNRLVALLIS
                     GRHKAVAPNCPFDFTKNQHVGGFFGNIFKAANVSGYGPGWVQTNTCVRI"
     gene            complement(1799..2752)
                     /locus_tag="VNP73_02880"
     CDS             complement(1799..2752)
                     /locus_tag="VNP73_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64895.1"
                     /translation="MAHLSDRGKRDIPDLFDGDPLIAAFVARARRVLPEPVAEDTARI
                     HLALIRRPVPTEIVEVVASGATPTWRRAMSRTASIGMKIAAATAALLLSTIGLAYAGV
                     DLPGTAAEKAVEKVLGVELPNQAADDSASDEAHGKSDEDHGKSDEAGDEENGKSVSDD
                     VHDVKDSTEERGCEFGQAVSDAAGANRQGDEDNEKDPCDREDGEEHEPQGSRATGEEH
                     SADKGNEDHATKDDHPTKDDKPVNDNEPANDNEPKGGKTTGDDHRSDKPADDDDTEAA
                     EEEGESQGSQDTGVDHSAGAGDKAEEAVANGHANKPTDGDD"
     gene            complement(2779..3333)
                     /locus_tag="VNP73_02885"
     CDS             complement(2779..3333)
                     /locus_tag="VNP73_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWL64896.1"
                     /translation="MDDDRFAAVLSAARTGDSSAWEEIYKNLSPIVMGYLRSRRAPDP
                     EDLVGAVFLDLVRNLSRFQGNERDFRTWTLTIAHRRLVDALRKAKRRDAEIYTDRISE
                     AQVPSGDTETEALDQLGSAQVRELLGGLSPDQQDVLLLRILGDLTIDETAEVLGKRPG
                     AVKALQRRGLAALKRKLGRLPPYP"
BASE COUNT          529 a         1128 c         1044 g          716 t
ORIGIN      
        1 cgaagttcag tactcgcgaa cctctgtaaa cgaggacgat gcccacagcc agcagcgcgt
       61 acacgccgcc gttggctaag cccgagatga gcgcctgact caccgttacc tacctgtccg
      121 tgtcgagttc gagtcagatg cgaacgcagg tgttggtctg gacccaaccg gggccgtagc
      181 ccgacacgtt cgcggccttg aatatgttgc cgaagaaccc tccaacgtgt tggttcttcg
      241 tgaagtcaaa gggacagtta ggagccactg ctttgtgtct gcctgagatc aacagcgcga
      301 ccaaccggtt gcggtcgcag ttcggcccac agtcgagcag ccactggtgg acctgcttca
      361 tcgcaagcca ggtcatccac aggatgtcgt tgcctttctg cgcgcgcccg tactggcgct
      421 gcgcggcctc gtaacgcttg atctcgtccg cgtacgcgga gaacggtcct ttggtcgcgc
      481 cgggcgcata agcgggccac atcgcgatcc cctcgaccgg cgggttgagc atgtcttcct
      541 tgagcgtgtc cgtcatgacc tggaacggga agacaaccca cgtggggtgg tagtcctgat
      601 tcttcgcttg cttgatcacc tcgatgtcgg cgagcccgtt ctcccacagg aacaccgtct
      661 cggcccccct cgactggagc tctgcgatct gctgcgtgta gacggcctgg tccttctgaa
      721 cggggaggtc ggcgacgatg tcgaggccgg cgcgcttcgc agcagcaagg aacgtcttgt
      781 gacccggctg ccacgcctca ctctgacgcc acaggatgcc gatcttgtcg ttgcggtgct
      841 tggcaacgat ccatttggcg gcgagaagac cggactgctc gaccgacggc aggaagctga
      901 agctgtacct cttgtccaga tcctcgcgcg cgatgtggtg cacgtagagc aggcggttct
      961 gttcctgctc gttccacgcc cgcacggccg ggatctgatc gaacccgatc ccgccgataa
     1021 gcatgaaggg gttcttggcc ttgcaacgct ccgcggcctg aaccgcgttg tcgggccggt
     1081 acgcgtcgtc ctcgatcgtg agttgcacgt tgcggccgta gatgccgccg tggtcgttaa
     1141 ccatcgacca gtaaacgttg atgtcttcgg ggcgcgtatc gaaagcctcg gcgaaagtca
     1201 gggccgcgtg gccacacagg gtgatctggt cttccgtaaa gcccatctgc gcggtgctgt
     1261 ccggccacag gttcacgttg ggaagaccct tccccagacg atgggcgcgt tccgcctgct
     1321 ctttctcgct gaccgtttcc ccggtggccg agacggtgtt tccgtcggtg gcatcggccg
     1381 ggatgccgcc tccgccggtg cttccaccgc cggagccgga cgcaccgagg ccgcccccac
     1441 cggacttcga accgccgagc ttgccgcccg aagacttacc gccgaggttg ccccccgatg
     1501 agtcgtcgag gatctggccc ggcgactgac tctgtgaggc ttgagggacg ggccctcccg
     1561 agcacgccgc tccgccgatc agcgccacca gcgccaccac gaatacccac cggatctccg
     1621 tcaaccgccc catgccgccg cccccttcga accacagccc gctttatgaa caagcgttca
     1681 tataccaagt ttgggcaatc taggtcaaat cgagcacgta aaagagcccc cgagcggggt
     1741 gccgggggct ctttaattcc gctgctggcg gacagcggag gcttatgaac gttgcttctt
     1801 agtcgtctcc gtccgtcggc ttgttcgcgt ggccgttcgc gacggcctct tcggctttgt
     1861 ccccggcgcc ggccgaatgg tccacgccgg tgtcctggct gccctgggac tcgccttctt
     1921 cttcggcggc ttcggtgtcg tcatcgtcgg ccggcttgtc ggagcggtgg tcgtcaccgg
     1981 tggtcttgcc gcccttcggc tcgttgtcgt tcgccggctc gttgtcgttc accggtttgt
     2041 cgtctttcgt cgggtggtcg tccttggtgg cgtgatcctc gttgcccttg tcggcggagt
     2101 gctcctcgcc cgtggcgcgg ctcccctgcg gctcgtgctc ctcgccgtcc tcccgatcac
     2161 aggggtcctt ctcgttgtct tcgtccccct ggcggttggc gccggcagcg tcggagacgg
     2221 cctgaccgaa ctcgcagccc cgctcctccg tcgaatcctt cacgtcgtga acgtcgtcag
     2281 acaccgactt gccgttctcc tcgtcgccgg cctcatctga cttgccgtgg tcctcgtccg
     2341 acttgccatg tgcttcgtcg gaagccgaat cgtcggctgc ctggttcgga agctcgacac
     2401 ccaaaacctt ctcgacggcc ttctcggccg cggttccggg cagatcgacg ccggcgtatg
     2461 cgagtccgat cgtgctgagc agcagggctg cggtagcggc ggcgatcttc atgccgatcg
     2521 atgcggttct gctcatagct ctcctccatg ttggggtggc tcccgaagcg accacctcga
     2581 cgatctcggt tgggacgggc cgcctaatca aggcaaggtg tatccgagcc gtgtcttcag
     2641 cgaccggctc gggaaggacc cggcgggcac gcgccacgaa agccgctatg agcggatcgc
     2701 cgtcgaacaa gtccgggatg tctcttttgc cgcgatctga aaggtgtgcc atcgatccag
     2761 taatcgtcgg agcgggccct aggggtacgg cggcagtcgc ccgagtttgc gtttcagcgc
     2821 cgcgagcccc ctcctctgca gggccttgac cgccccgggt cgtttcccca acacctctgc
     2881 ggtttcatcg atggtgaggt cgccgaggat ccgcagaagg agcacgtcct gctggtcggg
     2941 cgaaagacca ccgagtagct ctcgcacctg ggcgctaccg agctgatcca gcgcctcggt
     3001 ctccgtatcc ccgctgggta cctgcgcttc ggagatcctg tccgtataga tctcggcatc
     3061 tcgacgtttc gctttgcgca aagcatcgac cagccggcgg tgagcgatcg tcagggtcca
     3121 ggtccggaag tcccgctcgt tcccttggaa tcgggacagg ttcctaacga ggtctaggaa
     3181 aaccgctccg accagatcct ctggatccgg agcgcgcctg ctcctgagat aacccatgac
     3241 tatcggcgac aggttcttgt agatctcttc ccacgccgag gagtcaccgg ttcgggccgc
     3301 gctgagtact gctgcgaaac gatcgtcgtc catcgcccgc ctaacgtgcg aggtgccggt
     3361 ccgttataga gaagagatcg tctgtttcag tccaacatcc aaaagccgtg gctgata
//
LOCUS       DATMED010000114         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5452122, whole genome shotgun sequence.
ACCESSION   DATMED010000114 DATMED010000000
VERSION     DATMED010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5452122"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VNP73_02890"
     CDS             complement(<1..777)
                     /locus_tag="VNP73_02890"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWL64897.1"
                     /translation="MARAAGDVLVSVTGDGATPVGGVTLDSRRVSHGDLFFCVPGLLH
                     DGHEYAAAAVEAGAAALVVERPLGLGVPEVQVKDARVAMARIGAEFYGRPGDDLMLLG
                     VTGTSGKTTTTWLLEPILAAAGHVAGLIGTIETRIAGVSSPGVRTTPDSLDLQRTLAE
                     MRSAGVSAVALEVTSHALALHRVETLHFEAAAFTNLSQDHLDFHAGMEDYFAAKRSLF
                     VPERIERGAINVDDAYGRVIKESAGVEMIGFGIAPDADVRA"
     gene            complement(828..>2006)
                     /locus_tag="VNP73_02895"
     CDS             complement(828..>2006)
                     /locus_tag="VNP73_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWL64898.1"
                     /translation="FEKDSGGLQLPQADSSYEQPEPGHSILLTIDKDIQYFTETALMN
                     AVTQFNAASGTAIVMRPKTGEILALANLPDYDPNSYNKFSDDERRNRALADVYEPGSI
                     FKAITLAGALNERAVKPTTTFDVPDQLQVADRIIHDSSPHAAEKMNVREIITESSNVG
                     TVKIGQKLGAEGLDEYVHRFGFGKKTGLDFPGESPGIVRALEDWSGSSIGNIPIGQGV
                     AVTPLQMVSSFATIANDGVWVEPRLLTGTVGDDAKLTPATASSKKRVVRAGVARTVTD
                     ILSNVVSDGTGLHAQIPGYEVAGKTGTAQKVLEEGGYGDDYFASFAGYAPARRPEVAA
                     IVILDSPTPIYGGLTAAPTFKIIVQRALRELAVPPSNNAEKAARAIEADEDSSLPAQD
                     "
BASE COUNT          344 a          648 c          629 g          385 t
ORIGIN      
        1 ggctcggaca tccgcgtcgg gagcgatccc gaaaccgatc atctcgacgc cggccgactc
       61 cttgatgacc cggccgtaag cgtcgtcgac gttgatggcc ccgcgctcga tgcgctcggg
      121 cacgaacagc gaccgcttcg ctgcgaagta gtcctccatt cccgcgtgga aatcgagatg
      181 atcctgactg aggttcgtga aggccgccgc ttcaaaatgc agcgtttcaa cgcgatgtag
      241 tgcgagcgcg tgcgaggtga cctcgagcgc gaccgcgctg accccggccg accgcatctc
      301 cgccagggtt cgttgaagat ccagcgagtc cggcgtggtc ctgacgcctg gactggagac
      361 tcccgcgatg cgtgtctcga tggttccgat cagcccggcg acatggcccg cggccgcgag
      421 gatcggctcg agcagccagg tcgttgtggt cttgccgctc gtgccggtaa ctcccagcaa
      481 catcaggtcg tcgccggggc gcccgtagaa ctcggcgcct atgcgcgcca tcgctacccg
      541 cgcatctttt acctgcacct cggggactcc aagtccgagt gggcgttcga cgacaagagc
      601 cgcggccccc gcctcgactg cggccgctgc gtactcgtgc ccgtcgtgca gcagccccgg
      661 tacgcaaaag aagagatcac catgggacac acggcgcgag tcgagcgtga cgccgccgac
      721 gggggtggcc ccatcgcccg taaccgagac aagaacatcg ccagcggccc gcgccaaagt
      781 acctagtttc tggatggttg gggcctcagg gctcatgggc aacgagccta gtcctgagcc
      841 ggcagcgagc tgtcctcatc cgcttcaatc gccctcgcag ccttctctgc gttgttggag
      901 ggcggaacgg cgagctccct caacgcacgc tgcacgatga tcttgaaggt cggagccgcg
      961 gtcagcccac cgtagatggg cgtcggcgaa tcgaggatca cgatcgccgc gacctcgggc
     1021 ctcctggccg gcgcatatcc ggcgaagctg gcgaagtagt cgtcgccgta gccgccctcc
     1081 tcgagcacct tctgggccgt tccggtcttg cctgcgactt cgtagccggg gatctgcgca
     1141 tggaggcccg tgccatcgct aacgacgttc gagaggatgt cggtcacggt tctggcaacc
     1201 ccggccctaa caactctctt cttggacgat gccgtggcgg gagtgagctt tgcgtcgtcg
     1261 ccgacggtac cggtgagcag ccggggctcg acccagactc cgtcgttcgc gatcgtggcg
     1321 aacgacgaca ccatctgcag cggtgtaacc gcaacgccct ggccgatcgg tatgtttcca
     1381 atgctcgaac ccgaccagtc ttcgagcgca cggacgatcc ccggtgactc gcctggaaag
     1441 tcgagtcccg tcttttttcc gaacccgaaa cgatgaacat attcgtcgag gccctccgcg
     1501 ccgagcttct gcccaatctt caccgttccc acgttcgagg actctgtgat gatctctcta
     1561 acgttcatct tctccgccgc gtgtggcgac gagtcgtgga tgatccggtc cgcgacttga
     1621 agttgatcgg gaacatcaaa ggtcgtcgtc ggcttgaccg cgcgctcgtt gagggcaccg
     1681 gcaagcgtta tcgccttgaa gatggaacca ggctcgtaga catcagcgag ggccctgttc
     1741 cgtctctcgt catcggagaa cttgttgtaa gagttgggat cgtagtcggg aaggtttgca
     1801 agcgcgagga tctcgcccgt cttcggccgc atcacgatgg ccgtccccga agcggcattg
     1861 aactgagtga ccgcgttcat cagcgcggtc tcggtgaagt actggatgtc cttgtcgata
     1921 gtgagcagga tcgagtggcc gggctccggc tgttcgtagg acgagtccgc ctgcggtagt
     1981 tggaggcccc ccgagtcctt ctcgaa
//
LOCUS       DATMED010000115         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5452266, whole genome shotgun sequence.
ACCESSION   DATMED010000115 DATMED010000000
VERSION     DATMED010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5452266"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="lnt"
                     /locus_tag="VNP73_02900"
     CDS             <1..1119
                     /gene="lnt"
                     /locus_tag="VNP73_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWL64899.1"
                     /translation="NVPVVGFTWGQLAQSQHNVTHLLPIAGLGGAWALSGLVAAVNAS
                     AAAALVKARDAKLRTALPAGIVAVALLVAPFLLPRAEASGPSLSVAIVQGNVPRPFVG
                     TVDEKSLVIFSSHADLTETLADSGVDLVVWPESAVGVDMNQNEVVAKRLSEAARSAGA
                     EMIVGGNLIAGDGLYEVLAFHVAPTGEIVDRYQKTHLVPFGEYVPGRRFLGWIPMLQQ
                     VQGDAVAGNEPKVFNLDQGPVAPVISFEGDFGSLVRDRIGDAGGRLLVVATNTSTWED
                     SWASAQHVAFSKLRAAENGVWVIHAALSGISAFVAPDGTVVKETGLWTEEILRADVRF
                     ATEPTLYARTGDWAPLACLAATAGLLLVAVRRRRGSVA"
     gene            1120..1860
                     /locus_tag="VNP73_02905"
     CDS             1120..1860
                     /locus_tag="VNP73_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWL64900.1"
                     /translation="MKSLVIVPTYNERDAIPEVIRRLFDAADPDTHLLVVDDGSPDGT
                     AAIVKELAAGPHEIELLERNGKQGLGTAYVTGFKWAFDRGYEAVVEMDADLSHDPADV
                     PRLLAGLDQAELAIGSRYVPGGGVGNWGRARRILSAGGNLYARAWLRYKVKDSTSGFR
                     AYRTDFLSTLELDSVSSEGYAFQIEMTRRVSRAGGRIVEVPITFIERAHGKSKMSRKI
                     VAEALVRVAGWGIKDMFGKKTAGRRPPM"
     gene            1857..2336
                     /locus_tag="VNP73_02910"
     CDS             1857..2336
                     /locus_tag="VNP73_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64901.1"
                     /translation="MSQHGLGTEQTYSSTNLFLTILGILSVAVPVGVAWRFTAAAGAP
                     AGILVMGLILVTLILLLSRVAVSLGPDAIGIELGPIGWRVLEIPYEQVEAVEVIRASP
                     AKWNGWRTGLFWPLSNGIIVRSGEAVLIKRSGGEVVVVTVDRASQAVNTIRERAGLA"
     gene            2336..>2922
                     /locus_tag="VNP73_02915"
     CDS             2336..>2922
                     /locus_tag="VNP73_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64902.1"
                     /translation="MKRISIALLTVVVATLAACDGGPLPGESVAAATEKAKAAGTAKV
                     FISMEQQMPSGQGTLSMEGDGAFDFERQAGMLTLSMSGLGGAAGAPQMPPMDMEMVFE
                     GFVMYMRLPTLQQVLPAGKTWVKLDLAKMSEQAGIDFEQLAQMGQNNPAQGLEYLQGA
                     TDIQELGSDEVRGVSTTHYSGVIDMDKLAEEADAAA"
BASE COUNT          530 a          864 c         1010 g          518 t
ORIGIN      
        1 aacgtccctg tggtcggctt cacgtggggt caactggcgc agagccagca caacgtgacc
       61 catctcttac cgatcgccgg tctcggcggt gcatgggcac tctcggggct cgtagcggcc
      121 gtcaacgcat cggcagccgc agcgttggtg aaggcgagag acgcgaagtt gcgaaccgcc
      181 ctcccggcgg ggatcgtcgc ggtcgcgttg ctcgtcgcgc cgttccttct accgcgtgcc
      241 gaggcgtccg gaccgtcgct ctcggttgcc atcgtccaag gcaacgtgcc gcgcccgttc
      301 gtaggaaccg tcgacgagaa gagtctcgtc atcttctcca gccacgcgga cctcaccgag
      361 acgctcgccg atagcggagt ggacctcgtc gtgtggccgg aaagcgcggt aggtgtcgac
      421 atgaaccaga acgaggttgt ggcgaaaagg ttgagtgaag ccgcgcgctc ggccggggcc
      481 gagatgatcg tgggagggaa tctgatcgcc ggcgacgggc tttacgaggt gctcgcgttc
      541 cacgtcgcac cgaccggcga gatcgtcgat cgctaccaga agacccatct cgttcccttc
      601 ggagagtacg tgccggggcg gaggttcctc ggctggatcc cgatgctgca acaggttcaa
      661 ggcgacgccg tcgcaggcaa cgagcccaag gtcttcaacc tggaccaagg cccggtcgcg
      721 ccggtcatct ccttcgaggg tgacttcgga tcgctcgtac gcgaccggat cggcgacgcc
      781 ggtggccgtc tcctcgtcgt cgcgaccaac acgtcgacgt gggaggactc gtgggcctcg
      841 gcacaacacg ttgcgttctc gaaactgcga gccgccgaga acggtgtctg ggtgatacat
      901 gctgcgttga gcggcatatc ggcgttcgtc gcgcccgacg ggaccgttgt gaaggagacg
      961 gggctatgga ccgaggagat cctgcgcgcc gatgtcagat tcgcgaccga gccgacgctg
     1021 tatgcgcgca cgggggattg ggccccgctg gcctgcctag ccgcaactgc cgggctgctt
     1081 cttgttgcag tgagacgcag gcggggtagc gtcgcatgag tgaagtctct cgtgatcgtt
     1141 ccgacctata acgagcgcga cgcgatccct gaagtgatcc gcaggctgtt cgacgccgcg
     1201 gacccggaca ctcatctgtt ggtcgtggac gatggctctc cggatgggac ggccgcgatc
     1261 gtcaaggagc ttgcggcagg gccgcacgag atcgagctgc tggagcgcaa cggaaagcaa
     1321 gggctcggaa ccgcgtacgt gacgggcttc aagtgggctt tcgaccgcgg ctacgaggcc
     1381 gtcgttgaga tggatgccga tctctctcac gatcctgccg acgtccctcg gttgctggcc
     1441 ggtctcgatc aagccgagtt ggcgatcgga tcgcgctacg tccccggcgg gggagtgggc
     1501 aattggggcc gggctcgacg gatcctctcg gccggcggca atctctatgc ccgtgcgtgg
     1561 ctccgctaca aggtcaaaga ctcgacgagt gggttcaggg cataccgaac cgattttctc
     1621 tcgaccctgg agctcgacag tgtgtcctcg gagggctacg ccttccagat cgagatgacc
     1681 cgacgggtga gcagggcggg gggcaggatc gtagaggtgc cgatcacctt catcgagagg
     1741 gcccacggca agtcgaagat gagccggaag atcgtggccg aggcgttggt aagagtcgcc
     1801 ggatggggta tcaaagacat gttcggtaag aagaccgccg gcagaaggcc accgatgtga
     1861 gtcagcacgg gttgggtact gagcagacct actcgtccac gaatctcttc ctcaccatcc
     1921 tgggcatcct ctccgtcgcc gttcccgtag gcgtggcatg gcgattcaca gccgctgccg
     1981 gtgcccccgc cgggatcctg gtcatgggcc tcatcctcgt cacgctgatc cttctgctga
     2041 gtcgcgtggc cgtgagtttg ggccccgacg cgataggcat cgagttggga ccgatcgggt
     2101 ggcgcgtgct cgagatcccc tacgaacagg ttgaagcggt cgaggtcata cgcgctagtc
     2161 ccgcgaagtg gaatggctgg cgcaccggcc tgttctggcc gctgagtaac ggcatcatcg
     2221 tgcgcagcgg agaagcagtg ttgatcaaga ggagcggcgg tgaagtcgtc gtcgtaacgg
     2281 tcgatcgtgc gtcgcaggct gtgaacacga tccgagagag agcggggctt gcctgatgaa
     2341 acggatctcg atcgcgctac tgactgttgt cgtggcgacc ttggctgcgt gcgacggggg
     2401 accgctgccg ggcgaaagcg ttgcagccgc aacagagaaa gcgaaggcgg cgggcaccgc
     2461 gaaggtgttc atatcgatgg aacaacagat gccttcggga caaggcaccc tgtcgatgga
     2521 gggcgatggc gccttcgact tcgaacgtca ggcaggcatg ctgacgctct cgatgtcggg
     2581 gctgggcgga gccgcgggag cgccgcagat gccgccgatg gacatggaga tggtcttcga
     2641 gggtttcgtg atgtacatga ggctgcccac gctccagcag gtcctgcccg ccgggaaaac
     2701 gtgggtcaag ctcgatctcg ccaagatgag cgagcaggca ggcatcgact tcgagcaact
     2761 tgctcagatg ggtcagaaca acccggccca gggcctcgaa tacctacagg gggccaccga
     2821 catccaggaa ctcgggagcg atgaggttcg cggcgtgagc accacccatt actcgggcgt
     2881 gatcgacatg gacaagctcg ccgaggaagc cgacgcggca gc
//
LOCUS       DATMED010000116         5292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1625192, whole genome shotgun sequence.
ACCESSION   DATMED010000116 DATMED010000000
VERSION     DATMED010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625192"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VNP73_02920"
     CDS             complement(<1..716)
                     /locus_tag="VNP73_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64903.1"
                     /translation="MDFALTEEQLELKKWAHEFAEKEIRPVAGEYDESEEFPWPVIKK
                     AAEAGLYGLDLYLQSQQDGTGLMLPIITEELTWGCAGIGLAILGSGLPLSALAAVGTQ
                     EQLFEWAPKMFGTVEDPKVGAFAVTEPQAGSDVRALRTKAVRDGDEWVLNGTKVFITN
                     GGVADVYVVVATVDAELGHRGQASFVIHKDDGGIRPGKKEKKLGIRASDTSEVILEDC
                     RVPLDRVLGGMDKLEKKLDSA"
     gene            829..2133
                     /locus_tag="VNP73_02925"
     CDS             829..2133
                     /locus_tag="VNP73_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HWL64904.1"
                     /translation="MTDAIESSGGAPAPDPTSKTEVISYERLYRLWEENNWSASALDF
                     SVDAQHWAERLTERQREAALWNYSMFLVGEEAVARTLTPIMDAAPDYPQQIFLATQIV
                     DEVRHHVFFDRFMREVASIGGDTKSTLHAVEGYLTWGFKQVFAELDRVTDMLRKKPKH
                     RPLLTQAVTLYHLVIEGMLAIPGQHFIQRYVEKLEILPAFSKGMTNVSRDESRHVAFG
                     IKFLGDLIRSSGECRAACIEELERVTRYTTGVFVPPNYDDSYAECFDFTLEEIYAFGF
                     RSLVTKLNRVGIDASEVRLLRSQDIELSYEERSRRLWVLIRAGILGDETREPEVTPEC
                     FEILFDSMARMMDLETARSLGGPIEWDFTDNEPWHLVVTNGHAEAKPGRAGDPALRLE
                     ISSADWAKIAVDRIDPRLALLKRRLKLHGSLSAKRKLPRLFA"
     gene            2143..3306
                     /locus_tag="VNP73_02930"
     CDS             2143..3306
                     /locus_tag="VNP73_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64905.1"
                     /translation="MDLDLSPEQQEFQKVVRSFVDDAIRPNAQRWDEEEELPLDAVKQ
                     MGELGLFGLPYPEEYGGGGSDFLTVCLAIEELARVDSSLAITLEAAVGLGSMPIYRFG
                     TEEQKQKWLVPLAQGEALGAFGLTEVGGGSDAGATRTTAKLVDGEWVINGTKVFITNS
                     GTDITLCTTITAVTGESDGRRLVSAVIVPNGTPGFEVAPQYKKMGWRHSDTHELVFSD
                     CRVPEENLLGEPGEGFRKFLAILDDGRIAIAALAVGLAQGCLDESIAYAKERHVFGRP
                     IAGFQAIRFKIADMAMKTELARQAYYRAAWLKEMGRPYKLEASMAKLYASEIAVDVAR
                     DAVQIHGGYGFMEEYPVCRHFRDSKVLEIGEGTSEVMRMLIARELGLPEEWER"
     gene            3294..4481
                     /gene="rnd"
                     /locus_tag="VNP73_02935"
     CDS             3294..4481
                     /gene="rnd"
                     /locus_tag="VNP73_02935"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HWL64906.1"
                     /translation="MGALAAAGQLVTTTEELQRALGAVRDSGIIGIDTEFMREKTYRA
                     KLCLVQFSADEDVWLLDPLSTVSLAPLGEVVGEPAIKKVVHAGKQDLEIFYETFNVVP
                     ANVYDVQVAAAFAGYGASLAYGRLVSAALGVSLTKGESYTDWCRRPLTDAQLRYAADD
                     VRYLIPLYDKLTKALTEAGRLDWIDDEFAELGDEETYRFDPASVWKKVGGRGSLTPRQ
                     TAVLREVARWREETAMKRDLPRGWIVKDATLIDVARRAPSSLGELKSVRGMNAREVER
                     GGQEILAAIEKGRRGEPVDKASSPPRTVQARARMVSGLADAVVRARCERAGLATELVA
                     TRSELENLLTEVFDGRLNERSHRILQGWRRELAGDAVVDLAEGKLAVRVLNDPPYVEE
                     VYI"
     gene            4501..>5292
                     /locus_tag="VNP73_02940"
     CDS             4501..>5292
                     /locus_tag="VNP73_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWL64907.1"
                     /translation="MNELGEDRIALFLDFENLAIGAKERGGKLDMSVIMDALSERGRI
                     VVRRAYADWNLFSEHRAGLVAERIELIEVPQRTGVVRKNAADIKLAVDAMELAFQRDF
                     LTTFVIASGDSDFTPLVLKLRELNKKVIGVGVEGSTSELLPGACDEFLFYERLLGHGS
                     GKGAPRRTRSKAADNGGAASGEADSDLAEVARLVTSTLSGLQSSSDGPVLSSMIKRVI
                     LRKDPTFAESDFGFRAWGELMRHLADEGIIELLEGTAEGDPSVDFP"
BASE COUNT         1001 a         1590 c         1727 g          974 t
ORIGIN      
        1 gcactgtcga gcttcttctc gagcttgtcc atcccgccga gaacgcggtc taggggaacg
       61 cggcagtcct cgaggatgac ctcagacgtg tcggaggcac ggatcccgag cttcttctct
      121 ttcttaccgg gacggatccc accgtcgtcc ttgtggatga cgaaggaagc ctggccgcgg
      181 tggccgagct cggcatccac cgttgcgacc acgacgtaga cgtcggcgac gcctccgttc
      241 gtgatgaaca ccttggttcc gttcaagacc cactcgtcgc cgtcgcgcac ggccttggtc
      301 ctaagggctc gcacgtccga tccggcctga ggctcggtga ccgcgaacgc tccgaccttg
      361 gggtcttcta cggttccgaa catcttcggg gcccactcga agagttgttc ctgggtaccc
      421 accgcggcca atgccgataa ggggagtccc gacccgagga tcgccaagcc gatgccggcg
      481 cagccccacg tgagctcctc ggtgatgatc ggaagcatca gtccggtccc gtcctgctgg
      541 gactggagat agagatcgag gccgtacagc cccgcctcgg ccgccttctt gatgacgggc
      601 caggggaact cctcggattc gtcgtattcg ccggccacgg gccggatctc cttctcggcg
      661 aattcgtggg cccatttctt gagttccagt tgttcttcgg tcagcgcgaa atccatctac
      721 cgtgctcctg ttgtccgggg actcgaacgt ttcgctcaac ttgaccgctc ggtcaagttt
      781 cgtatgagga aggatagacc agattccgaa tcttcaggag gtcaagcagt gacagacgcc
      841 atcgagtcca gcgggggcgc cccggccccc gacccgacct ccaagaccga ggtcatctct
      901 tacgagcggc tctaccgcct gtgggaggag aacaactgga gcgcctctgc tctcgacttc
      961 tcggtggatg cccagcattg ggccgagcgt cttaccgaac ggcaacgcga ggcggctctg
     1021 tggaactact cgatgttcct cgtgggggag gaagccgtcg cccggaccct tactccgatc
     1081 atggacgcgg cccccgacta tccgcagcag atcttccttg cgactcagat cgtcgatgaa
     1141 gttcgccacc acgtgttctt cgaccggttc atgcgcgagg tcgcgtcgat cggaggagac
     1201 acgaagtcga ccctccatgc cgtcgagggc tacctgacgt gggggttcaa acaggttttc
     1261 gccgagctcg acagggtcac ggatatgttg cgcaagaagc cgaagcaccg gccgttgctc
     1321 actcaggcag taacgctcta tcacctcgtg atcgagggga tgcttgcgat ccccgggcaa
     1381 catttcatcc agcgctacgt cgagaagctc gagatccttc ccgccttctc gaagggcatg
     1441 accaacgtgt cgcgcgacga atcgcgccac gttgcgttcg gcatcaagtt cctgggggat
     1501 ctgatccgtt cgtcgggcga gtgcagggcc gcttgcatcg aagagctcga gagggtcacc
     1561 cgctacacca ccggagtctt cgtcccccct aactacgacg actcttacgc cgaatgcttc
     1621 gacttcacgc tcgaggagat ctacgcgttc ggtttccgct cgctagtgac caagttgaac
     1681 agggtgggga tcgacgcttc cgaggtacgt ttgctgcgca gtcaagacat cgaactgtcg
     1741 tacgaagagc gatcgaggcg actgtgggtc ctcatcaggg ccggcatcct cggcgacgag
     1801 accagggagc ctgaagtcac tcccgaatgt ttcgagatcc ttttcgacag catggcgcgc
     1861 atgatggatc tggaaaccgc gcgttccctg ggtggcccta tcgagtggga tttcaccgat
     1921 aacgagccgt ggcacctcgt cgtcacgaat ggtcatgcgg aagcgaagcc gggccgcgcc
     1981 ggcgatccgg cgttgcgcct ggagatctcg tcggccgact gggccaagat cgcagtcgac
     2041 cggatcgatc cacgactcgc attgctgaag agacgtttga agttgcacgg cagtctttcg
     2101 gccaagcgca aactcccgag actcttcgcc taagctcgct gcatggatct agacctttca
     2161 cctgaacagc aggagttcca gaaagtcgtc cgctctttcg tggacgacgc gatccgtccc
     2221 aacgctcagc gctgggacga agaggaggaa ctccctctcg acgcggtcaa gcagatggga
     2281 gagctaggtc tgttcggcct tccttacccc gaggagtacg gcggaggggg gtccgatttc
     2341 ctgaccgtct gtctcgcgat cgaggagctt gcccgggtcg actcgtcgct ggccatcacg
     2401 ctcgaagccg ccgtcggatt gggttccatg ccgatatacc gcttcggtac tgaagaacag
     2461 aagcagaagt ggctcgttcc actcgcccag ggcgaggcgc tgggggcctt cggcctgacc
     2521 gaggtgggcg gtggatccga cgccggcgcg accaggacga cggccaagct cgtggacggt
     2581 gaatgggtca tcaacgggac gaaggtcttc atcacgaact ccgggacgga catcacgctc
     2641 tgcaccacga tcaccgcggt aacgggggag agcgacggca gacggctcgt ctccgccgtc
     2701 atcgttccga acggcacccc cggtttcgag gtcgcccctc agtacaagaa gatgggttgg
     2761 cgacactccg acactcatga gctcgtcttc agcgactgcc gggttccgga agagaacctt
     2821 ttgggagaac ccggggaggg tttccgcaag ttccttgcga tcctcgacga cggccgcatc
     2881 gcgatcgcgg cactggcggt cggcctcgct caaggctgct tggacgagag catcgcctac
     2941 gcgaaggagc gccacgtatt cggccggccg atcgccgggt tccaggcgat ccgtttcaag
     3001 atcgccgata tggcgatgaa gaccgagctt gcgcgccagg cctactaccg ggcggcatgg
     3061 ctcaaggaga tggggcgtcc ctacaagttg gaagcctcga tggccaagct gtacgcatcc
     3121 gagatcgcgg tggacgtcgc ccgcgacgcg gtccagatcc acggtggata cgggttcatg
     3181 gaggagtacc ccgtgtgcag gcacttccgc gactcgaagg tgttggagat cggtgagggg
     3241 acgtctgagg tgatgcgcat gctgatcgcg cgtgaactgg ggcttcccga ggagtgggag
     3301 cgctagccgc ggccggacaa ctcgtcacca cgacagaaga actccaacgc gcccttgggg
     3361 ccgttcggga ctcggggatc atcggtatcg acaccgagtt catgcgcgag aagacgtatc
     3421 gggcaaaact ctgtctcgtc caattctcgg ccgacgaaga cgtgtggctg ctcgaccctc
     3481 tatccacggt ctcgctcgct ccgctcggcg aggtggttgg agagcccgcg atcaagaagg
     3541 tcgttcacgc cggcaaacag gatctagaga tcttctacga gacgttcaac gtggtgccgg
     3601 ccaatgtgta tgacgttcag gtggcggcgg ctttcgccgg ctacggcgcg tcactcgcat
     3661 acgggagact cgtgtcggcc gcgctgggcg tgtcgctgac gaagggcgag tcgtacaccg
     3721 actggtgccg gaggccgctg accgacgccc agctgcgtta cgcggccgac gacgtcaggt
     3781 acctcattcc gctgtacgac aagctcacga aggcactcac cgaagccggg aggctcgact
     3841 ggatcgacga cgaattcgcc gagctcggag atgaagagac ctatcgcttc gacccggcaa
     3901 gcgtttggaa gaaggttggg ggccggggtt cgctcacgcc gcgtcagacc gccgtattga
     3961 gagaggtagc gcgctggcga gaggagacgg cgatgaagcg cgatctgcct cgaggttgga
     4021 tcgtgaagga cgccaccttg atcgacgtcg ctcggcgagc cccctcttcg ctcggtgagt
     4081 tgaagagcgt ccgaggcatg aatgcgagag aggtggagcg agggggccag gagatcctgg
     4141 ctgccatcga gaaaggccgc cgcggtgaac ccgtggacaa ggcgagctcc ccgccccgaa
     4201 ccgttcaggc ccgagcccgg atggtgtcgg gcctcgccga cgctgtggtt agggcgcgct
     4261 gcgagcgagc cgggctggca acagagctgg tggccacgag gtccgagctc gagaatctgt
     4321 tgaccgaggt gttcgatgga cgactgaacg aacgatcaca ccgcatactt caagggtggc
     4381 gccgtgagct ggccggggat gccgtcgtcg atctggccga ggggaagctg gccgtcaggg
     4441 tgctgaatga cccaccctac gtagaagagg tctacatctg aaacaaaccg aagggatgcg
     4501 atgaacgaac taggtgagga tcgcatagcg ctgttcctcg acttcgagaa cctagcgatc
     4561 ggcgcgaagg aacgcggcgg gaagctcgac atgtcggtga tcatggacgc gctcagcgag
     4621 cgaggccgga tcgtcgtacg acgggcgtat gccgactgga acctgttctc ggagcaccgg
     4681 gccggcctcg ttgccgagcg gatcgagctg atcgaggttc cgcaacgtac cggcgtcgtg
     4741 cggaagaacg cggcggacat caagcttgcc gtcgatgcga tggagcttgc tttccaacgg
     4801 gacttcctga cgacgttcgt gatcgcgtcg ggtgactcgg acttcacacc gctggtgctc
     4861 aagctgcgag agctgaacaa gaaagtcatc ggagtcggcg tggagggctc gacgtccgag
     4921 ttgcttccgg gcgcgtgcga cgagttcctc ttctacgagc gtctactcgg ccacggatcg
     4981 ggcaagggag cgcctcgccg cacgcgcagc aaggccgccg ataacggtgg ggcagcgagc
     5041 ggggaagccg actcggatct tgccgaggtg gcacggctgg tcactagcac gctttcgggg
     5101 ctgcagagtt cgagcgacgg acccgttctg tcatcgatga tcaagcgggt catcttgcgc
     5161 aaagacccca cgttcgcgga gagcgacttc ggattcagag cgtggggcga attgatgcga
     5221 catcttgcgg acgaggggat catcgagcta ctcgagggaa ccgccgaagg ggatccgagc
     5281 gtcgacttcc cc
//
LOCUS       DATMED010000117         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4699804, whole genome shotgun sequence.
ACCESSION   DATMED010000117 DATMED010000000
VERSION     DATMED010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4699804"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VNP73_02945"
     CDS             complement(<1..262)
                     /locus_tag="VNP73_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64908.1"
                     /translation="MNRILRSAIFYLILIIAVVAIFNFYRDSVDSPQVLESVNDFQSR
                     METGTITTAKFLTKDEKVVGDCECDEGATKFEVFVPKDTVDDW"
     gene            complement(374..925)
                     /gene="hpt"
                     /locus_tag="VNP73_02950"
     CDS             complement(374..925)
                     /gene="hpt"
                     /locus_tag="VNP73_02950"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWL64909.1"
                     /translation="MHKDIEKILITADEIQDKITELAKQITEEYRDKDLLLVGVLKGA
                     FVFMSDLARQIDLPIEFDFMAVSSYGTATKSSGVVRILKDLDYEITDRHVLLVEDIID
                     SGLTISYLLRYLEARRPASLEICSLFWKKGEQAVPLDVKFPGFEIPPVFVVGYGLDYA
                     EKYRNLPYIGVLKSVVYPTPNPL"
     gene            complement(1083..2078)
                     /gene="tilS"
                     /locus_tag="VNP73_02955"
     CDS             complement(1083..2078)
                     /gene="tilS"
                     /locus_tag="VNP73_02955"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWL64910.1"
                     /translation="MKPYWKPYGGPGFDLLEKCEKTIAAHRMLEHGQTVCVALSGGPD
                     STCLLDVLARLGEKLDLTLIIAHADHQLSDETAEVAARISSAAAQQGYEVHLARAPDL
                     EGPNLHARARDFRYEFFEIVSEREGADRIATGHTLDDRVETTIARLIHGSGTSGLAGV
                     PPVHGRRVRPLIDIRRSETRAYCTEVGLDFFDDPANSSRRFERSAIRQDIVTAIESRW
                     GDGAIRAMGRSSELLRQDAEALSHLAETIYADLGVDAGTGVRFPREGLSALPRAFRRR
                     LLELAVGRVRDRGGGIEAAVEAMEERSSRREVRFSVASGIEISIVGDDVLVSKLD"
     gene            complement(2086..3138)
                     /locus_tag="VNP73_02960"
     CDS             complement(2086..3138)
                     /locus_tag="VNP73_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HWL64911.1"
                     /translation="MAKLIEPKIASAVARALSGDSGTRGSYLLDRLSRDLDYAVPKAE
                     SLVAEASGIDAPPPVRWGVIDRASWARANIRGMVTMLEPLAERLGRRMEQLPMPARVA
                     QTTAVSVEVGALLGFVSRRVLGQYDLLVTDEDPHSTPLYFVGVNMVEIEREFGFIPEE
                     FALWVAIHEVTHRFQFEGVPWLRAHFLSLVHSYLGSVELDAKGLAQRFAGALRRLAEK
                     GVPPEEKSPVYLLASDEQRRLIGQVQALMAVVEGHGNYVMDTVGKEVLGSFERMRSVF
                     TRRRDQTGAFQRAIGHVIGLEMKLRQYELGQVFCNEVVAAEGSEALAHLWKDPSHFPT
                     MEELREPRLWLDRVAA"
BASE COUNT          636 a         1045 c          947 g          603 t
ORIGIN      
        1 cccagtcgtc gaccgtgtct ttggggacga agacctcgaa tttggtcgcc ccctcgtcgc
       61 attcgcagtc gccgacgacc ttctcgtcct tggtaaggaa cttggcggta gtgatcgtcc
      121 cggtttccat cctgctctgg aaatcgttaa ccgactcgag cacctgagga ctgtcgacgc
      181 tgtcgcgata gaaattgaag atcgcgacga cagcgattat gaggatgaga tagaagatcg
      241 ccgatctgag gatgcgattc atgttgtccc aaggttagca gcggtgtacg cctaaaaaac
      301 cgatgaaaag ggttgtttat ttcagggttt tcatcaccct tcggcgtcgc cggtggagct
      361 cttgagtctc acctcaaagc gggttcggtg tcgggtagac gacagatttc agaaccccga
      421 tgtagggcag gtttcggtac ttttcagcgt aatccaaccc gtaccccacc acgaacacgg
      481 gagggatctc gaacccgggg aacttgacgt cgagtggcac ggcttgctcg cctttcttcc
      541 aaaagagcga gcagatctcc agagaagcgg gtcgccgggc ctccagatat cgcaacaggt
      601 aagagatcgt caggcccgaa tcgatgatgt cctcgacgag caacacgtgc cggtcggtga
      661 tctcgtagtc aaggtccttg aggatccgaa cgacccccga ggatttcgtg gccgtcccat
      721 acgacgagac cgccatgaag tcgaactcga tcgggaggtc tatctggcga gcgaggtccg
      781 acatgaatac gaaggcgcct ttgaggacac cgaccagaag gagatccttg tcccggtact
      841 cctccgtgat ctgtttcgcc agctcggtga tcttgtcctg gatctcgtcg gcggtgatga
      901 ggatcttctc gatgtccttg tgcacttccg tcaattgtcc tccctcaggt atgcccgctg
      961 cgcgggcagc ccttcacttc ggacggcgcg atcgtcgaga cgatcgctca ctgtcctccc
     1021 tcaggtatgc ccgctgcgcg ggcagccctt cacttcggac ggcgcgatcg tcgagacgat
     1081 cgctagtcga gtttactcac gagaacgtcg tcaccgacga tcgagatctc gatccccgac
     1141 gcgaccgaga aacggacctc gcgtcgcgac gatcgttcct ccatcgcctc caccgccgcc
     1201 tcgatcccac caccccgatc acggacgcgc ccgaccgcaa gttcgagcag ccgccgacgg
     1261 aaggcacgcg gaagcgcgga caatccctcc cgcgggaagc gaacgccggt cccggcatca
     1321 acaccaagat ctgcgtagat cgtttcggcc agatgggaca gagcctcggc atcctgtctc
     1381 agcagttcgg acgaccggcc catcgctctg atcgccccgt cgccccaccg cgactcgatc
     1441 gcggtaacga tgtcttgcct gatagcagag cgctcgaagc gacggctgct attcgccggg
     1501 tcgtcgaaga agtcgagccc gacctctgtg caatacgccc tcgtctccga gcggcggata
     1561 tcgatcaacg gccgcaccct gcgtccgtga acgggaggca ctccggccaa cccggacgta
     1621 cccgaaccat ggatcaagcg cgcgatcgtc gtttcgacgc gatcgtcgag cgtgtggccg
     1681 gttgcgatcc gatcggcccc ctcccgttcg gagacgatct cgaagaactc atagcggaag
     1741 tcacgtgcgc gtgcgtgcag attcggaccc tccaagtcgg gagcgcgtgc gaggtggacc
     1801 tcataaccct gttgcgcggc agcactcgag atacgcgcgg caacttcggc cgtctcgtca
     1861 gagagctgat ggtcggcatg ggcgatgatc agagtcaggt ccagcttctc ccccaaccgg
     1921 gcgaggacgt cgagcaaaca ggtggagtcc ggcccccccg acagagcaac acaaacggtt
     1981 tgtccgtgct cgagcatgcg gtgggccgcg atcgtcttct cgcacttctc gagcagatcg
     2041 aagccgggac ctccgtacgg cttccaatac ggcttcatga cgatctcacg cggctacgcg
     2101 atcgagccat aacctcggct cgcgtagctc ctccatcgtc gggaagtgcg aggggtcctt
     2161 ccacagatga gctagtgctt cgctgccctc ggctgcaacc acctcattac agaagacctg
     2221 ccccaattcg tattgccgca acttcatctc taagccgatc acatggccga tcgcgcgctg
     2281 gaaggctccc gtttgatccc gacgacgcgt gaacaccgag cgcatccgct cgaacgagcc
     2341 caggacttcc tttccgacgg tgtccatgac gtagttgccg tggccctcga ccaccgccat
     2401 cagcgcctgg acctgtccga tcaggcggcg ttgctcgtca ctggcgagca ggtaaacggg
     2461 gctcttctcc tccggcggca cacccttttc ggcaagacgc ctcagggctc ccgcgaaccg
     2521 ttgagccaga cccttggcat ccagctcgac cgagcccaga tacgagtgga cgagagacag
     2581 gaaatgagcc cgtagccacg gaacgccttc gaactggaag cgatgcgtca cctcgtggat
     2641 ggcgacccac agagcgaact cctcgggaat gaacccgaac tcgcgctcga tctcgaccat
     2701 gttcaccccg acgaaataca gaggggtgct atgggggtcc tcgtcggtca ccaacaggtc
     2761 gtactggccg agaacccgtc tggaaacgaa gccgagcaag gctccaactt cgacggacac
     2821 cgcagtcgtc tgagcaacgc gggccggcat cggaagctgc tccatcctcc ggccgaggcg
     2881 ttcggccaga ggctcgagca tcgtgaccat ccctcggatg ttcgctctcg cccaactcgc
     2941 tcgatcgatc acgccccacc ggacgggagg cggggcgtcg atgccgcttg cctctgcgac
     3001 gaggctctcg gccttcggga cggcgtagtc cagatcgcgc gacaggcgat ccaagagata
     3061 ggagccgcgc gtccccgagt caccacttag agcgcgcgcg accgccgatg cgatcttcgg
     3121 ttcgatcagt ttcgccaccg cacctccggc gacgtagtta gtggggcact aagcgttgcc
     3181 cctctggcga gcggcgatca tccggtagta gaggaaggcg gttcccgcga g
//
LOCUS       DATMED010000118         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2400776, whole genome shotgun sequence.
ACCESSION   DATMED010000118 DATMED010000000
VERSION     DATMED010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400776"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VNP73_02965"
     CDS             <1..951
                     /locus_tag="VNP73_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64912.1"
                     /translation="TTDASGRFEFAGLATGEERSYAVDARFQGGFFAGRPVRLPPDTQ
                     QEPVIEATLRVWETTTDPAAVLIARDDMFAVLGKQGIAIIEAVTIVNQTDMAYIGRGG
                     GSSGEAKASFGFSLPVECRASGVAIVDSDIDIPEIVCTDFGFGITSAIPPGETRTTIS
                     YQVPGATSTFDLSRTALYDVSSFSVFAADPLELETNRLEKEEEVTLAGERYERWTSTA
                     PVDPGDALQVVALAKAGTEPGLYIGIAAAIVFLSALLLFALRHRRRAGADDAEPPKGQ
                     EVVHAIARLDLAYRNGDLTHHDWSMKRRELKQRLSHPQPD"
     gene            951..1604
                     /gene="ccmA"
                     /locus_tag="VNP73_02970"
     CDS             951..1604
                     /gene="ccmA"
                     /locus_tag="VNP73_02970"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWL64913.1"
                     /translation="MRVEIDHLAVAFGRTLALDDVELEIGPGITGLFGPNGSGKSTLL
                     RAICGLIRPSSGRVLLDGTSMTLKNEGLRARIGYAGHESGLYPQLTLVENLTLFARLY
                     GISDGRVGSVIDDLALHHERETQVSALSAGTKRRAAVARALLHDPDLLLLDEPYANVD
                     DDAADRVTAAIVRWSSSDKIAIVATHGAKKVRPYATSGVVMQRGRVARYGAYAGQPA"
     gene            1601..2323
                     /locus_tag="VNP73_02975"
     CDS             1601..2323
                     /locus_tag="VNP73_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWL64914.1"
                     /translation="MKATGFFGKTYALTVKDLRIEMRAKETLPPMLAFAVIVSLLLAF
                     TLPAVPAPVSPARLPVGAVEAADVLAGFIWVTILFAGLIGFARTFEFERREAALDSML
                     LAPLDRSGLFMAKALANLVFIVAVEVFVFAAFFIFFSLEISNASLLFTIALLADIGFI
                     SVGTLFAALAAQTRSRELILPILALPALVPVFIAAVTLTADLIAGVPVSAVAGRGWFG
                     ILIAYDVIMTTIAALAFEAAVE"
     gene            2398..>3254
                     /locus_tag="VNP73_02980"
     CDS             2398..>3254
                     /locus_tag="VNP73_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64915.1"
                     /translation="MKKIALLVALVLVAGSCGGRQQVAERRELKPRPLTLPDPIRALP
                     AFRLTFSKTPHRQVVDRLLKIRGVAVAVPIDKGSVMVKAGDRKARLRVGSASALLYRA
                     IAPSVTAQAEFVWSSLLQGEAVLTPETASRLGMTGASAVEIKGRTFTVGAFADNGVPN
                     IADVLLSDHVAKKLDLRPAAIVVGAKPKHSLERMRAAIRSEVPAARLHRLQPRPDPPA
                     PEPVGTAEGGLIGAMTFRIRKNGFIEPDPAWVSANIVEADVPVLGHVMCHRLVIPQLG
                     AALAEIERKR"
BASE COUNT          572 a         1043 c         1042 g          597 t
ORIGIN      
        1 acgaccgatg cttcgggtcg tttcgagttc gccggcctcg ccaccggaga agagaggtcg
       61 tacgcggtcg acgcccgctt ccaaggagga ttcttcgccg gacgtccggt cagattgcca
      121 cccgacactc agcaggagcc cgtcatagaa gcgacgctcc gcgtatggga gacgacgacc
      181 gatccggccg cagtcttgat cgcgagagac gacatgttcg ccgtgctcgg caagcaaggc
      241 atcgcgatca tcgaggcggt gacgatcgtg aaccagaccg acatggccta cataggcagg
      301 ggcggaggta gctcggggga agcaaaggca agcttcgggt tctcgctccc tgtcgagtgc
      361 cgcgcctcgg gcgtggccat cgtcgactcg gacatcgaca tccccgagat cgtctgcacc
      421 gacttcgggt tcgggatcac aagtgccatc ccacccgggg aaacgcgtac gacgatctcg
      481 taccaggtcc cgggagcaac gagcacgttc gatctctccc ggaccgcgct gtacgacgtg
      541 tcctccttct cggtattcgc ggccgacccc ctcgagctcg agacgaacag gctcgaaaag
      601 gaggaggaag tcaccctcgc cggggaacgc tacgagcgct ggacgagcac cgcgccggtc
      661 gatcccgggg acgcgctgca ggtcgtggcg ttggccaaag ccggcaccga gccgggcctc
      721 tacatcggga tcgcggccgc catcgtgttc ctgagtgcgc tcctgctttt cgctctcaga
      781 cacagaagac gagcgggagc ggacgatgcc gagcctccga aagggcaaga ggtagttcat
      841 gcgatcgctc gtctcgatct ggcgtacagg aacggtgatc tgacccacca cgactggtcg
      901 atgaagcggc gcgagttgaa acagcgtctc tcccaccccc agcccgactg atgcgcgtcg
      961 agatcgatca cctcgctgtt gctttcgggc gcaccctcgc gctcgacgat gtcgagttgg
     1021 agatcggtcc cggcatcacc ggtctgttcg gaccaaacgg atcgggcaag tccaccttgc
     1081 tccgagcgat atgcgggctc atcagacctt cgagcggacg ggtgctcctg gacggaacgt
     1141 cgatgactct caagaacgag ggactgagag cccggatcgg ttatgcgggc cacgagtccg
     1201 gcctctaccc ccagttgacg ctggtcgaga acctgacgct gttcgcccgg ctgtatggca
     1261 tctccgacgg ccgtgtcggt tcggttatcg acgacctcgc gctgcatcac gagagggaga
     1321 cacaggtctc ggccctttcc gccggaacca aacgcagggc ggcggttgca cgcgccctcc
     1381 tacacgatcc ggatctcctc ttgctcgacg agccgtacgc gaacgtcgac gacgacgctg
     1441 ccgatcgggt gacggcagcc atcgtgcgct ggtcttcgtc ggacaagatc gcgatcgtcg
     1501 caacccacgg agcgaagaaa gtccggccct acgcgacctc gggtgtcgtc atgcagcgag
     1561 gtcgggtcgc tcgatacggc gcctacgcgg ggcagcccgc atgaaggcca cagggttctt
     1621 cggtaagacc tatgcgctga ccgtgaagga cctccgtatc gagatgcgcg ctaaggaaac
     1681 gctgccgccg atgctcgctt tcgcggtgat cgtctccctc ttgctcgcct tcaccctccc
     1741 cgccgtgcca gctcccgtgt ctcccgcgcg actgcctgtt ggggccgtcg aagcagccga
     1801 cgtgctggcc gggttcatct gggtcacgat cctgttcgcc ggcttgatcg ggttcgcgcg
     1861 cacgttcgag ttcgaacgcc gagaagctgc actcgactcg atgttgctgg caccactcga
     1921 taggtcggga ctgttcatgg ccaaggcact ggccaacctc gtgttcatcg tcgccgtcga
     1981 ggtattcgtc ttcgcagcct tcttcatctt cttctcgctt gagatctcga acgcgagtct
     2041 gttgttcacg atcgccctgc tcgccgacat cgggttcatc tcggttggca cgctgttcgc
     2101 cgccttggca gctcaaacgc gcagccgcga gctgatcctg ccgatccttg cgttgcccgc
     2161 gctggtgccg gtcttcatcg ccgcagtgac cctgacggcc gacctgatcg ccggagtgcc
     2221 ggtcagtgcc gtcgcaggga gagggtggtt cggcatcctc atcgcctacg acgtgatcat
     2281 gacgacgatc gcggcgctcg cgttcgaggc ggccgtcgag tgagcgatcg tctcgacgat
     2341 cgctccgtcc ggaacgaagg gctgccccgc ggagcgggca tacctgaggg aggacaaatg
     2401 aagaagatcg cgctgctcgt cgccctggtc cttgtcgccg gttcttgtgg tggtcgtcag
     2461 caagtcgcgg agaggcggga gttgaaacca cggccgttga ccctccccga tccgatccgc
     2521 gctttgccgg ccttcagatt gacgttctcg aagacacccc atcggcaggt cgtcgaccgg
     2581 ttgctgaaga tccggggcgt cgcggtcgcc gttccgatcg acaaggggtc ggtgatggtg
     2641 aaagcgggtg atcggaaagc tcggttgaga gtgggctccg catcggcctt gctgtacaga
     2701 gcgatcgcac cgagcgtgac tgcacaggcg gagttcgtgt ggtcctcact gctgcaaggc
     2761 gaagcggtcc taacacccga gaccgcgtca cgtctcggaa tgacaggagc ttctgccgtc
     2821 gagatcaagg gaagaacgtt cacggtcggt gccttcgccg ataacggcgt acccaacatc
     2881 gccgacgtcc tcctgtccga tcacgtggcg aagaaattag atctgcggcc cgccgcgatc
     2941 gtggtgggag cgaagccgaa gcactccttg gagcggatgc gcgctgcgat cagatccgag
     3001 gtgcccgctg cccggctgca tcgactgcaa ccgcgccccg acccaccggc tcctgaaccc
     3061 gtcggcaccg cggaaggcgg actcatcggt gcgatgactt tccgcatacg gaaaaacggg
     3121 ttcatcgagc ccgatccggc ctgggtgtcg gcaaacatcg tcgaggccga cgtgccggtc
     3181 ctcggacacg tgatgtgtca caggttggtg atcccgcaac tgggggccgc gctggcagag
     3241 atcgagcgga agcg
//
LOCUS       DATMED010000119         6758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2401118, whole genome shotgun sequence.
ACCESSION   DATMED010000119 DATMED010000000
VERSION     DATMED010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401118"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VNP73_02985"
     CDS             <1..150
                     /locus_tag="VNP73_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64916.1"
                     /translation="QVPLAEMFGYATDLRSKTQGRATYTMQFNSYQEVPQSIAEDIIA
                     TVRGE"
     gene            188..1378
                     /gene="tuf"
                     /locus_tag="VNP73_02990"
     CDS             188..1378
                     /gene="tuf"
                     /locus_tag="VNP73_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWL64917.1"
                     /translation="MGKAKFERTKPHVNVGTMGHIDHGKTTLTAAITKVLSESNPNTA
                     FTAFDQIDKAPEEKERGITIAIAHVEYETENRHYAHVDMPGHADYIKNMITGAAQIDG
                     AILVVSAADGPMPQTREHVLLARQVNVPFIVVALNKVDMVDDPELLDLVELEVRELLT
                     EYEYPGDDTPVTRVSALKALDGDADAAQGILDLMKTVDEYIPEPERDTDKPFLMPIED
                     VFTITGRGTVVTGRVEQGVLKVGEEIEIVGIDEKTSKTVVTGIEMFRKMLDEAQAGDN
                     AGILLRGTKKEEVQRGQVLAKPGSVTPHTKFTAQVYILSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGSIKLPDGTEMVMPGDNTEMEVELGQPIAMDEGLRFAIREGGRTVGAGRVV
                     KITE"
     gene            1425..1724
                     /gene="rpsJ"
                     /locus_tag="VNP73_02995"
     CDS             1425..1724
                     /gene="rpsJ"
                     /locus_tag="VNP73_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWL64918.1"
                     /translation="MKIRIRLKAYDHEVIDQSAKKIVETVTRTGASVNGPIPLPTERS
                     VYTVIRSPHKDKDSREHFEMRTHKRLLDIVNPTPKTVDSLQRLDLPAGVDIELKL"
     gene            1745..2413
                     /gene="rplC"
                     /locus_tag="VNP73_03000"
     CDS             1745..2413
                     /gene="rplC"
                     /locus_tag="VNP73_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWL64919.1"
                     /translation="MAEVKGILGRKIGMTQVFDEAGHAVPVTVVEAGPCHVAQIKRPE
                     TDGYSAVQLSFGAARRLSKPAAGHLQKAGIENGRHLVELRIEDMAGYELGTEIKADIF
                     AEGELVDVVGVTKGKGFAGGMKRHNFKGLSQSHGTKRKHRSPGSIGACATPARVFKGT
                     RMAGQLGNRRVTTLNLMVIKADPERNLLLIRGAVPGPRGGLVMVRSAVRLRRRGGGSE
                     RKAG"
     gene            2577..3242
                     /gene="rplD"
                     /locus_tag="VNP73_03005"
     CDS             2577..3242
                     /gene="rplD"
                     /locus_tag="VNP73_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004805682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWL64920.1"
                     /translation="MAETTTKAKKLTAQVIDGTGKSSGTKDLPAEIFGGAINEALLHQ
                     VMTAELAEARSGTASTKTRSEVRGGGKKPWRQKGTGRARHGSIRSPQWVGGGRVFGPK
                     PRKYSVKVNKKMRVAALRSALADKANGSAIWVMDGFTETKTKAAGSALSAAGIDGRIL
                     IVLDPEDEGSRTVDRAFRNLDKVAFSLQGSLSTYDVLVADAVLFTSTAFDRFLARAKG
                     GRG"
     gene            3239..3544
                     /gene="rplW"
                     /locus_tag="VNP73_03010"
     CDS             3239..3544
                     /gene="rplW"
                     /locus_tag="VNP73_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWL64921.1"
                     /translation="MSGSTKPGPRDVILAPVVSEKSYSLLDMNAYTFLVHPRANKTEI
                     RKAVEEIWGVKVMSVNTVNRKGKTKRFRYTQGRRRDTKRAVVRLVEGDKIEIFEQGG"
     gene            3548..4378
                     /gene="rplB"
                     /locus_tag="VNP73_03015"
     CDS             3548..4378
                     /gene="rplB"
                     /locus_tag="VNP73_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWL64922.1"
                     /translation="MPIRKAKPTSAGRRFQSFADYSELTRREPEKALVEPKPKKAGRN
                     SYGRITTRHQGGGNKNRYRVIDFRRNKDGVPAKVAHIEYDPNRNARVALLHYADGEKR
                     YILLPNRVRVGDTLQSGPKADVSPGNALPLRNIPVGTVVHNVELKPGGGGKMARSAGS
                     SVQVVAKEGKNATLRLPSGEMRMVPLDCRATVGEVGNSEAELVSGGKAGRARWKNKRP
                     SVRGVAMNPVDHPLGGGEGKSSGGRHPVSPWGQPEGRTRKKKPSDQLIVRRRRKKRGR
                     "
     gene            4381..4662
                     /gene="rpsS"
                     /locus_tag="VNP73_03020"
     CDS             4381..4662
                     /gene="rpsS"
                     /locus_tag="VNP73_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWL64923.1"
                     /translation="MPRSLKKGPFVDDHLLIKVTEMNKRNEKKVIKTWSRRSTVIPEM
                     VGHTIAVHDGKKHVPVYVTEAMVGHKLGEFAPTRAFRTHGSSERAAKVK"
     gene            4665..5333
                     /gene="rplV"
                     /locus_tag="VNP73_03025"
     CDS             4665..5333
                     /gene="rplV"
                     /locus_tag="VNP73_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWL64924.1"
                     /translation="MARKIAPDAQEAIATARFIRMSPTKARQVVDLIRGRHVADARRV
                     LRFTSRAASLPVGKVLESAVANAEHNRGLPTDELVVVRTWVDEGPTLRRYRPRAQGRA
                     TRIRKRTCHISVVVGRQEDEVVLPERPATRQTARRVRKDTAGKPRKPAATKTSTAEEA
                     APKKTSRKKTAKKTTAKKTTAKTTTAKTTAKKTTAKKTAARKKTTRAAGDKSSSPEKK
                     RNDS"
     gene            5333..6253
                     /gene="rpsC"
                     /locus_tag="VNP73_03030"
     CDS             5333..6253
                     /gene="rpsC"
                     /locus_tag="VNP73_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWL64925.1"
                     /translation="MGQKVHPYGFRLGIHTDWKSRWFTEKQYRDYLEADLRIREYLLG
                     QLPHAGISRIDIERTRERVRIDAHTARPGIVIGRRGSEAERLRSHLEHMESKIYGKPT
                     DVRLNIIEVKQPELDAQLIAQGVAEQLASRVSFRRAMKKAVGTAMRHGAKGVRVQCGG
                     RLGGAEMSRTEWYIEGQMPLHTLRADIDYGFAEAKTTFGRIGCKVWIYKGPFEDVYRV
                     ARDAARMRREAALAAGETVGRGEEERARRRGQTKAAATKKAPAAKRATGKAEASEGKA
                     SSEPEPPVETPPQPADDKPSSAGSSGQGDS"
     gene            6253..6678
                     /gene="rplP"
                     /locus_tag="VNP73_03035"
     CDS             6253..6678
                     /gene="rplP"
                     /locus_tag="VNP73_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWL64926.1"
                     /translation="MLQPKKTKHRKQHRGRMRGQAKGGTEVNFGDFGLMAVEPGWISN
                     RQIEAARIAMTRHIRRGGKVWINIFPDKPYTKKPAETRQGSGKGNPEGWVAVIKPGRV
                     MFEIAGIPEPVAREAIRLAAHKLPIKCKFVRREQPLEVQ"
BASE COUNT         1448 a         2069 c         2219 g         1022 t
ORIGIN      
        1 caggtaccgc tggccgagat gttcggctac gcaacggacc tccgcagcaa gacgcaaggt
       61 cgtgccacgt acacgatgca gttcaacagt taccaggaag ttccacagtc gatcgcagaa
      121 gacatcatcg caacagtccg gggcgagtaa cccaactcaa gaacagggaa ggaagaggga
      181 ggagccgatg ggaaaggcaa agttcgagcg cactaagccg cacgtaaacg tgggcacgat
      241 gggccacatc gaccacggta agacgacgct cacggccgct atcacgaagg ttctctccga
      301 gagcaacccg aatacggcgt tcaccgcctt cgaccagatc gacaaggctc ccgaagagaa
      361 agagcgtggc atcacgatcg ccatcgctca cgtcgagtac gagaccgaga accggcacta
      421 cgcgcacgtc gacatgcccg gtcacgccga ctacatcaag aacatgatca ccggcgccgc
      481 ccagatcgac ggcgcgatcc tcgtggtctc cgcagccgac ggcccgatgc cccagacgcg
      541 tgagcacgtg ctgctcgctc gtcaggtcaa cgtgcctttc atcgtcgtcg ccctgaacaa
      601 ggtcgacatg gtcgacgacc ccgagctcct cgacctcgtc gagctcgagg ttcgcgagct
      661 cctcaccgag tacgagtacc cgggtgacga cactcccgta accagggtct ccgccttaaa
      721 ggcactcgac ggggacgccg acgctgctca ggggatcctc gacctcatga agaccgtcga
      781 cgagtacatc cccgagcccg agcgcgacac cgacaagccc ttccttatgc cgatcgagga
      841 cgttttcacg atcaccggtc gtggcacggt cgtcacgggc cgcgtcgagc agggcgtgct
      901 gaaggtcggc gaggagatcg agatcgtcgg tatcgacgag aaaacgtcca agaccgtcgt
      961 tacgggcatc gagatgttcc gcaagatgct cgatgaagcg caggccggag acaacgccgg
     1021 gatcttgctg cgaggcacca agaaggaaga agtgcagcgt ggtcaggtgc tcgcgaagcc
     1081 cggttcggtt acgccgcaca cgaagttcac ggcgcaggtc tacatcttgt ccaaggacga
     1141 gggtggacgg cacacgccgt tcttcaacaa ctaccgcccg cagttctact tccgcaccac
     1201 ggacgtgacc ggctcgatca agttgcccga cggcaccgag atggtcatgc ccggtgacaa
     1261 caccgagatg gaagtcgagc tcggacaacc gatcgccatg gacgagggtc tgcggttcgc
     1321 catccgtgag ggtggccgca ccgtcggcgc cggccgtgtc gtgaagatca ccgagtagca
     1381 gcagcaacta ctagagaaaa gagtccactc acttggcatc ggggatgaag atcaggatca
     1441 ggctgaaggc ctacgaccac gaggtcatag accagtcggc gaagaagatc gtcgagaccg
     1501 ttacccgtac gggggcatcg gtcaacggcc cgatccctct accgaccgag cgctccgttt
     1561 acacggtcat ccgctcgccg cacaaggaca aggactcgcg cgagcacttc gagatgcgca
     1621 cgcacaagag gttgctagac atcgtcaatc cgacgcccaa gacggtcgac tcgctccagc
     1681 gactcgatct accggccggc gtcgacatcg aactcaagct gtaacgacgc agaaagaggt
     1741 ataggtggcg gaggtgaaag gcatcctcgg tcggaagatc gggatgacac aggtgttcga
     1801 cgaggcgggg catgccgtcc ccgttaccgt cgtcgaggcc ggcccgtgcc acgtagcgca
     1861 gatcaagcgg ccggagaccg acggctactc ggccgtccag ttgtccttcg gcgcagcccg
     1921 ccggctcagc aagccggccg ctggtcacct ccagaaggcc gggatcgaga acggccgtca
     1981 tctcgtcgag cttcggatcg aggacatggc cggttacgag ttggggaccg agatcaaggc
     2041 cgacatcttc gctgagggtg aactcgtaga cgtcgtcggc gtgaccaagg ggaagggttt
     2101 cgccgggggg atgaagcgac acaacttcaa aggtctctcc cagtcccacg ggaccaagcg
     2161 gaagcatcgc tcgcccggct cgatcggtgc gtgtgccacg ccggcccgtg ttttcaaggg
     2221 cacgcgcatg gcggggcagc tcggtaatcg ccgcgtcacg actttgaacc tgatggtcat
     2281 caaggccgat ccggagcgga acctcctgtt gatcagaggt gccgtccccg gcccccgagg
     2341 gggcctcgtg atggtgcggt cggcggtccg tctgcgccgt cgcggtggcg gctccgagag
     2401 gaaggccggg tagtggacct ccaagcaacg ttcgcgatgc gctgtcggct gcaaagcggc
     2461 gccatgctgc gttctcggtc gcgcacgcac ctttcaggtg cgcacgctcc ctgcggcctt
     2521 gcctggcttg cttttcgctc gacagacgca tgctcacgtc actcggaggt ccactgatgg
     2581 ccgagacgac tactaaggcg aagaagttga cggctcaggt catcgacggg accggcaagt
     2641 cctccggcac caaggatctg cccgccgaga tcttcggcgg cgcgatcaac gaggctttgc
     2701 tgcaccaggt catgacggcc gagctcgccg aggcccggtc cggaacggcc tcgaccaaga
     2761 cccggtccga ggtgcgcggg ggtggcaaga agccgtggcg tcagaagggc accggccgcg
     2821 ctcggcacgg atcgatccgc tcgccgcagt gggtgggcgg cggtcgcgtt ttcgggccga
     2881 agccgcgtaa gtactcggta aaggtgaaca agaagatgcg cgtcgctgcg ctgcgatcgg
     2941 cgctcgccga caaggcgaac ggctcggcga tctgggtcat ggacggtttc accgagacga
     3001 agaccaaggc ggctgggtcc gcgttgagtg cggcgggtat cgatggccgg atcctgatcg
     3061 ttctcgaccc tgaagacgag gggtcgcgga cggtcgacag ggccttccgt aatctggaca
     3121 aggtggcgtt ctcgttgcag ggatcgctct ccacctacga cgtgctcgtt gccgatgccg
     3181 tgctcttcac gtccactgcc ttcgacaggt tcctcgcgcg agcgaagggg ggccggggat
     3241 gagtggatcg accaagccgg gtcctcgcga cgtgatcctc gctcccgtcg tcagcgagaa
     3301 gtcgtattcg ttgcttgaca tgaacgcgta cacgttcctg gtccaccctc gtgccaacaa
     3361 gaccgagatc cgcaaggcgg tcgaggagat ctggggcgtc aaggtcatga gcgtgaacac
     3421 ggtcaaccgc aagggcaaga cgaagcgttt caggtacacc caaggtcgcc gtcgcgacac
     3481 gaagcgagca gtggttcgtc tcgtcgaggg cgacaagatc gagatcttcg agcagggggg
     3541 ctaacggatg cccatacgta aagcgaaacc gacatcggcg ggacgaaggt tccagtcgtt
     3601 cgccgactat tccgaactca ctcgtcgcga gcctgagaag gcactcgtcg agccgaagcc
     3661 gaagaaggcc ggtaggaact cctacggtcg catcacgacg cggcatcaag gtggcgggaa
     3721 caagaaccgc taccgggtga tcgacttccg ccggaacaaa gatggcgttc ccgccaaggt
     3781 cgctcacatc gagtacgacc cgaaccggaa cgcgcgtgta gctctcctgc attacgccga
     3841 cggcgagaag cgctacatcc ttctcccgaa ccgcgtgcgt gtcggcgaca cgcttcagtc
     3901 gggacccaag gcagacgtga gtccgggcaa cgctcttccg ctccgcaata tcccggtcgg
     3961 tacggtcgtc cacaacgtcg agctgaagcc cggcggcggg gggaagatgg cccgctccgc
     4021 cggatcttcc gtacaggtcg tagcgaagga aggcaagaac gccacgttgc gactgccttc
     4081 gggcgagatg cgcatggttc cactcgattg ccgtgcgacg gtaggcgagg tcggcaactc
     4141 cgaggcggag ctcgtttccg gcggcaaagc cggccgagcc cggtggaaga acaagcgtcc
     4201 gtccgtccga ggggtcgcga tgaacccggt cgaccacccg ctcggcggcg gggagggcaa
     4261 gtcgtccggt ggacggcacc cggtgtcgcc ctggggccag cccgaagggc gaacccgcaa
     4321 gaagaagccg tcggaccagc tcatcgtccg caggcgcagg aagaagaggg gtcgttagcc
     4381 atgcctcgct cattgaagaa aggtccgttc gtcgacgatc acctgctgat caaggtgacc
     4441 gagatgaaca agcgcaacga gaagaaagtg atcaagacgt ggtctcgacg gtccacggtg
     4501 atccccgaga tggtgggtca cacgatcgcc gtccacgacg gcaagaagca cgtgcccgtg
     4561 tacgtcaccg aggcgatggt cggtcacaag ctgggcgagt tcgctcccac acgagcgttc
     4621 cgcacccacg gctcgtcgga gagggcggca aaggttaagt aatcatggct aggaagatcg
     4681 caccagacgc acaggaagcg atagcgacgg cgagattcat ccgcatgtcg cccactaagg
     4741 cgcgccaggt cgtggacctg atccgcggtc gccacgtggc cgacgctcgc cgggtgcttc
     4801 gtttcacctc gagggccgca tctctgccgg tcggcaaggt tctggagtcg gcggttgcga
     4861 atgccgagca caacaggggt ctgcccacgg acgagctcgt ggtggtgcgt acctgggtcg
     4921 acgagggccc cacgttgcgc cggtaccgac ctcgagcgca gggccgagcc acccgcatcc
     4981 ggaagcgtac ctgtcacatc tcggtagtcg tcggacggca agaggacgaa gttgtgttgc
     5041 cggaacggcc ggcgacgagg caaaccgctc gccgggtgcg caaggacaca gcggggaagc
     5101 cgcgcaagcc ggcggcgacc aagaccagca cggccgagga agctgccccc aagaagacgt
     5161 cacgcaagaa gacggcgaag aagacgaccg caaagaaaac cactgcaaag acaaccactg
     5221 caaagacaac ggcaaagaag accaccgcga agaagactgc ggccaggaag aagacgacac
     5281 gggctgcggg cgacaagtcg tcctcgcccg agaaaaaaag gaacgacagc taatgggcca
     5341 aaaagttcat ccgtacggat tccgcctcgg tatccacacc gactggaaat cgcgctggtt
     5401 caccgagaag cagtaccgcg attacctcga ggccgatctc cggatccgcg agtacctgtt
     5461 gggccagttg cctcacgccg gcatctcgcg catcgacatc gaacgcacga gagagcgcgt
     5521 tcggatcgac gcgcacaccg cccgaccggg gatcgtgatc ggtcgacgtg gatctgaggc
     5581 cgagcgactt cgctcccacc tcgagcacat ggagtccaag atctacggca agccgaccga
     5641 cgtgcgtctg aacatcatcg aggtcaagca accggagctc gatgcgcagt tgatcgccca
     5701 aggtgttgct gagcagctgg cgagccgtgt ctccttccgc cgagccatga agaaagccgt
     5761 gggaacggcg atgcgtcacg gggctaaggg tgtccgcgtg caatgtggcg ggcgtctcgg
     5821 cggcgccgag atgagccgca ctgagtggta catcgaggga cagatgccgc tgcacacgct
     5881 gcgcgccgat atcgactacg gattcgccga ggcgaagacg accttcggac gcatcggatg
     5941 caaagtgtgg atctacaagg gtcccttcga agacgtctac cgggtggccc gagacgcggc
     6001 ccgcatgcgt cgagaagcgg cactggctgc cggtgaaacc gtcggtagag gcgaggaaga
     6061 acgcgcccgc cgccggggac agaccaaagc ggcagctacc aagaaagctc cggcggcgaa
     6121 acgcgcaacc ggcaaggcgg aggcctccga aggcaaagcg tcgtccgagc ccgagccgcc
     6181 cgtagaaact ccgccacagc ctgcggacga caagccgtcc tcggctggtt caagtgggca
     6241 gggagatagc tgatgttgca gccgaagaag accaagcacc gcaagcagca tcgcgggcgt
     6301 atgcgtggtc aggccaaagg tggcaccgag gtgaacttcg gtgacttcgg tctcatggcc
     6361 gtcgagccgg gctggatcag caaccgtcag atcgaggcgg ctcgtatcgc catgacgagg
     6421 cacatccgtc gaggcggcaa ggtttggatc aacatctttc ccgacaagcc gtacaccaag
     6481 aagccggccg agacccgcca gggatccggt aaggggaacc ctgagggctg ggtagcagtg
     6541 atcaaacccg gccgggtgat gttcgagatc gcagggatcc cggagccggt cgcgcgtgaa
     6601 gcgatccggc tggcggcaca caagctgccg atcaagtgca agttcgtccg tcgcgaacag
     6661 ccgctggagg tccagtagcc atggcgacgg cgaaggaact gcgcgacctg ccgctcgatg
     6721 agcttcggga tcggttggtc gaggtgaagg aggagttg
//
LOCUS       DATMED010000120         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3542769, whole genome shotgun sequence.
ACCESSION   DATMED010000120 DATMED010000000
VERSION     DATMED010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3542769"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1713
                     /locus_tag="VNP73_03040"
     CDS             157..1713
                     /locus_tag="VNP73_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HWL64927.1"
                     /translation="MTVSDETLATISTPDTVETRIGTLNFSDGAPSAETVAKVYDQLD
                     FSRGVEAFLSSFQGASLAAIRKGFLENGVQDNDFFLFSELMDSNSLFLTGNADTVYLW
                     GFVDLSDGPMVVDIPQLTLPSALLGTIDDMWFGWITDFGLPGPDRGHGGRYLLVPPGY
                     DGPLPDSGFHVSHSRTNVVTVVGRAFMIDNDPSGPVAEIKESWRVYPYAPGAEGTAVA
                     TYLAGKAPLAADAEIPETKFVEGSGKAFNTIAPNDYSYWELINEVVQEEPAPAGDPEI
                     LGQLAAVGIVKGKEFAPDERMRGILEDAVAVGNATARTALFAPREGEGEGFAYYEGSD
                     WRNPLFSGGYEFLDPPPQITAEGVVPAESDGARKLNSRIAFFYLATVITPAMCMHLTG
                     IGSQYLLATRDSDGEYLDGSKSYKLNLPPDIPFERFWSVMLYDNQTRSMLQTSQPKPD
                     LGSQSGTVAENDDGSIDIYFGPESPAGHEDNWLQIVPGKGFIPILRFYSPKASFFDKS
                     WRPSEIEVVS"
     gene            1745..2161
                     /locus_tag="VNP73_03045"
     CDS             1745..2161
                     /locus_tag="VNP73_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64928.1"
                     /translation="MLIALLAVLGVDLIVLVGFVAFVLTRKRWVKRQSGAFHGAARVA
                     SGEVDGLRTKWNRGYGRWVRDVRVWTKGPFLFRNEIVATEGLVDQRPAEPDEVKRLGD
                     HPSLIRVRLDGGTVELAANGADTELLLGPHRKERLP"
     gene            complement(2193..>2561)
                     /locus_tag="VNP73_03050"
     CDS             complement(2193..>2561)
                     /locus_tag="VNP73_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWL64929.1"
                     /translation="FGLQRFKVYGADPLPPGEHQLRIEFHYDGGGVGKGGDAKLYLDG
                     DEVGEGRIEMTVPMVFSADETTDVGSDSATPVTDDLGPKETEFTGRIDWVQIDIDEAA
                     EDVDHLISPEELLRVAMARQ"
BASE COUNT          442 a          877 c          805 g          437 t
ORIGIN      
        1 cggatccgcg ggtggcttgg gcagagccag taggcgattt cacccgcttc gggtgatgac
       61 cggtcactcc gtggcgcgga tagtgaatgc gaggttcggt acgcctgcga cccgcgtgtg
      121 ggggcgtgca cagatacgcg acaccaggag tgacacatga ccgtttccga cgagacgctt
      181 gcgaccatct cgacgcctga cacggtcgag acccgaatcg ggactctgaa cttcagcgat
      241 ggcgccccta gcgctgagac cgttgcgaag gtctacgacc agctcgactt ctcccgcgga
      301 gtcgaggcct tcctcagcag cttccagggc gcctcgctcg ccgcgatccg caagggcttc
      361 ctcgagaacg gggtccagga caacgacttc ttcctcttct cggagctgat ggattcgaac
      421 tcgctgttcc tcacggggaa cgccgacacc gtctacctct ggggcttcgt cgacctcagc
      481 gacggcccga tggtggtcga cataccgcaa ctgaccctgc ccagtgccct tctcggcacg
      541 atcgacgaca tgtggttcgg gtggataacg gacttcggcc tccccggccc agaccgcggc
      601 cacggcggca ggtacctact tgtccccccg gggtacgacg gacctctgcc cgacagcggc
      661 ttccacgtct cccactcccg caccaacgtg gtcaccgttg tcggccgcgc cttcatgatc
      721 gacaacgacc cgtccgggcc ggtggcggag atcaaggagt cgtggcgcgt ctacccctac
      781 gctccgggcg ccgagggaac cgcggtcgct acgtacctgg ccggtaaggc gccactcgcc
      841 gcagacgccg agatcccgga gaccaagttc gtcgagggga gcggcaaggc gttcaacacc
      901 atcgcgccca acgactactc ctactgggag ttgatcaacg aggtcgtcca ggaagagccg
      961 gcccccgccg gcgaccccga gatccttggc cagttggcgg ccgtggggat cgtcaagggc
     1021 aaggagttcg ctcccgacga gcggatgcgg ggaatcctcg aggatgcggt ggcggtcggc
     1081 aacgcaaccg cgcgcacggc gctcttcgcc ccccgagagg gggaaggcga gggcttcgcc
     1141 tactacgagg gctcggactg gcgcaacccg ctcttctccg gcggctacga attcctcgac
     1201 ccaccgccgc agatcaccgc cgagggcgtg gtcccggccg agtccgacgg cgcccggaag
     1261 ctcaactcgc gaatcgcctt cttctacctg gcgacggtga tcaccccggc gatgtgcatg
     1321 cacctgaccg ggatcggctc tcaatacctc ctcgccaccc gcgattccga cggcgagtat
     1381 ctcgacggct ccaagagcta caagctgaac ctgccccccg acatcccctt cgaacgcttc
     1441 tggtcggtga tgctctacga caatcagacc cgctcgatgc ttcaaaccag ccagccgaag
     1501 ccggacctcg gcagtcagtc gggcaccgtt gcggagaacg acgacggctc gatcgacatc
     1561 tacttcgggc ccgagtctcc cgccggccac gaggacaact ggttgcagat tgtgccgggc
     1621 aagggcttca tcccgatcct gcggttctac agccccaagg cgtcgttctt cgacaagtcc
     1681 tggcggccga gcgagatcga ggtcgtgagc tagcagggct caccgaggat cgcggaggtc
     1741 gccgatgctg atcgcactgc tcgcagtact cggcgtcgat ctcatcgtcc tcgtcggctt
     1801 cgtcgcgttc gtgctcacgc gcaaacgctg ggtgaagcga cagtcgggag cctttcacgg
     1861 ggccgcccga gtcgccagcg gagaggtgga tggcctccgt actaagtgga atcggggcta
     1921 cgggcgctgg gttcgcgacg tccgcgtctg gaccaagggg ccgttcctgt tccgtaacga
     1981 gatcgtcgcc acggaaggcc tcgtggatca gcgtccagcc gaaccggacg aggtcaagcg
     2041 tcttggcgac cacccatcct taatcagggt caggctcgac ggcgggacgg tggagctagc
     2101 ggcgaatggc gccgacaccg aactgctcct cggtccgcac cgcaaggaac ggctgcccta
     2161 gccggaagcg tcatcgctcg tgatcggtgc tgttactgcc gcgccatcgc gacccgcagc
     2221 agctcctcgg gcgagatcag gtggtcgacg tcctcggccg cctcgtcgat gtcaatctgg
     2281 acccagtcga tccggcccgt gaactcggtc tccttcgggc cgaggtcgtc tgtgacaggc
     2341 gtggcgctat ccgagccgac gtcggtggtc tcatcggccg agaagaccat cggaaccgtc
     2401 atctcaattc tgccctcgcc aacctcgtcc ccgtccaggt agagcttcgc gtcaccgccc
     2461 ttgccgaccc caccgccgtc atagtgaaac tcgatccgca gctggtgctc gcctggcggg
     2521 agcgggtcgg cgccgtagac cttgaaacgc tgcaagccga a
//
LOCUS       DATMED010000121         5323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3930223, whole genome shotgun sequence.
ACCESSION   DATMED010000121 DATMED010000000
VERSION     DATMED010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3930223"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VNP73_03055"
     CDS             complement(<1..1159)
                     /locus_tag="VNP73_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWL64930.1"
                     /translation="MSVTSVGAAELGEIGLGSTRLGRLIEAVRSYLPRGGSLPCKQWN
                     KRHHVMNIALWLHVPGLFILGVLNDHGPLHVLLECTVVAGAATIARVGGRRQNIAMIA
                     NTIGLLSASAILVHFTGGLIEMHFHYFVIVAAVTLYQAWLPFLVAIAYVVAQHGIIGV
                     LEPASVYNHPAAIANPWKWAVLHGLFIVGECVTLLIAWRLNEDSRHEAELSYQEKLEE
                     EQARREVQDRYGRIFESAVEGIYETTVDGVIVTANPSLARILGYESVEEFISSTTVPD
                     HYARPDDRIRFIQHLEKEDTITGFEFKARRRDGEEVWFSETARAVRDEEGNLVGIQGM
                     LEDITGRKRAEKKERELEAQLRQAQKMEAFGQLAGGVAHDFNNLLSVIENYA"
     gene            complement(1156..2250)
                     /locus_tag="VNP73_03060"
     CDS             complement(1156..2250)
                     /locus_tag="VNP73_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWL64931.1"
                     /translation="MNTNGAHQLLVVDDEEPARRLLARILQQDGYQCDQAADVTEALE
                     QVSRKEFALVITDLDMPGASGLELIRHLTSERPTVATMMVTGKGDREIAHRVVECGAY
                     GYLSKPLDPDEVVINVYNALLRRSLELENQQHRDKLEEMIKSRTSDLWETSLKLEHAL
                     AEAQDSQEETIRRLALAAELRDTETGAHNQRMSEYCHLLASRLEGVREIAETIRLGSL
                     LHDIGKIAVPDGILLKEGPLTHEERSIIEQHAEVGYQILKDSGSRLLQMGATIARTHH
                     EKCDGTGYPRRLTRAEIPIEGRIAAVADVFDALTSNRPYRTAYSVTQAGDIMREAAGP
                     HLDEEFVDLFLSDLRPILEIMETTGGAILI"
     gene            2600..3571
                     /locus_tag="VNP73_03065"
     CDS             2600..3571
                     /locus_tag="VNP73_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWL64932.1"
                     /translation="MGPIKIVTAALALAAALSTTATPAAGSAAAASNEWLDRRVLNIA
                     HQGGEIEAPSNTLFAFKTSLQKGSDVLELDVHATSDRELVVIHDATVDRTTNGTGRVD
                     QLTLDQIKELDAAHWFVPDCGTCHDEPGEAYAYRGFATGERPMRGRVARYEPNDFTIP
                     TLREVLETFPDTLINIEIKATAPDTIPYENELADLLREFERDSDTIVVSFLDHAVELF
                     DALAPEVDTATGTAQAGAFWASGQGPLPGSPSRHQALQVPIEFNGLTVVTEEFVAEAH
                     ANGLAVHVWTINDQETMEWLIEIGVDGIMTDRPELLEDVLKAQKVRI"
     gene            3607..4320
                     /locus_tag="VNP73_03070"
     CDS             3607..4320
                     /locus_tag="VNP73_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL64933.1"
                     /translation="MELTEGVFRLGSPYVNFYAIEEGGRLTIVDGGASGYWDEIPRLL
                     AATKLAITDIAAVVLTHSHGDHTGCVERLQAESGADVFIHTAEEAVLTGREKQAAPSG
                     AAGALLSLQGLKMIAHMLTNGGMKSTTLSTVETYTEDEVLDVPGKPRVVYTPGHSKGH
                     SALFVEQRDALFTGDALVTRDLRGRTGPRMMDINVDHAGARRALDRFEGIEAALLLPG
                     HGEPWRDGVAAAVERARRN"
     gene            complement(4317..>5323)
                     /locus_tag="VNP73_03075"
     CDS             complement(4317..>5323)
                     /locus_tag="VNP73_03075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL64934.1"
                     /translation="MLTWWEHCRPAGSRLCVITVMDEDRLAGIGPFFVTGGRVSRARL
                     LAAPVSNQLGPLALPAFERQTAATISAVLSSQGIPRLTLEGVKAAPPWGPIFGRSLSH
                     RVHKHWWMQAPIITLGEGTFDDWFASRSANFRQQMRRDRRKLEKAGASVRMTTEDSIE
                     SDVRSFSKLHRARWATRGGSATLSSTVEAMVLSAARRLIPVGAMRLWNIDVAGDTISS
                     HMFLTAGDVASYWLGGFDDGWSEMRPGMQALMAALEHSWSVGDKIFDLGSGDDPYKLR
                     FTDEHDTLEWWDVLELGRPGAWGVVLAERAKRSASRHLPEPTRKRVRAFLRSTRSRTD
                     "
BASE COUNT          979 a         1724 c         1632 g          988 t
ORIGIN      
        1 gagcgtagtt ctcgatgacc gacaacaggt tgttgaagtc gtgagccacg ccgccggcga
       61 gctggccgaa tgcctccatc ttctgcgcct gccgcagctg tgcttccaac tcacgttctt
      121 tcttctcggc gcgcttgcgg ccggtgatgt cttccaacat cccctggatg ccgacgaggt
      181 tgccttcttc atcccttacg gcccgggcgg tttcggagaa ccacacctct tccccgtcac
      241 gtcgccgtgc cttgaattcg aatccggtga tcgtgtcttc cttctcgaga tgctggatga
      301 accggatccg gtcgtccggt cgcgcgtaat ggtccggaac cgtcgtcgaa gagatgaact
      361 cttccaccga ctcgtatcca aggatcctgg cgagggacgg gttcgccgta acgatcacgc
      421 cgtcgaccgt tgtctcgtag atcccctcga cggcagactc gaagatccgc ccgtaacgat
      481 cctgcacttc acgccttgcc tgctcctctt ccagcttctc ttgatatgag agctcggcct
      541 cgtggcgaga gtcctcgttc aaccgccatg cgatcagaag agtgacgcac tcgccgacga
      601 tgaagagccc gtgcagcacc gcccacttcc agggattggc gatcgctgcg gggtggttgt
      661 agacgctggc cggctccaag actccgatga tcccgtgttg cgcgacgaca tatgcgatcg
      721 caacgaggaa cggcaaccac gcctggtaca gggtcacggc ggcaacgatc acgaagtagt
      781 ggaagtgcat ctcgataagt ccgccggtga aatggacgag gatcgcagac gcgctcaata
      841 gcccgatcgt gttggcgatc atggcgatgt tctgccgccg tccgcccacc cgggcgatcg
      901 ttgcggctcc ggcgacgacc gtgcactcca agagcacgtg aagcggaccg tgatcgttga
      961 gcacgccgag aatgaagagc ccgggaacgt gcagccacag cgcgatgttc atcacgtggt
     1021 gccgcttgtt ccactgcttg cagggcaacg atccgccgcg cggcagataa ctacggacgg
     1081 cctcgatgag cctacctaga cgtgttgaac cgagaccgat ctcgcctagc tcggcggcgc
     1141 caacgctcgt aacgctcata tcaggatcgc tcctcccgtg gtctccataa tctcgaggat
     1201 cggacggaga tccgacagga acagatcgac gaactcttcg tcgaggtgcg gtcctgcggc
     1261 ttctctcatg atgtcgccgg cctgcgtgac cgagtaggcc gtacggtaag gacgattgga
     1321 tgtcagagcg tcgaatacgt cggcaaccgc ggcgatgcgg ccttcgatcg ggatctcggc
     1381 ccttgtaagc cggcgcgggt atccggttcc atcacacttt tcgtgatgag tgcgggcgat
     1441 ggtcgcgccc atctgaagca gtctcgaccc cgaatccttg aggatctggt agcccacttc
     1501 cgcgtgctgc tcgatgatcg acctctcctc gtgcgtgagc ggcccctcct tgagcaagat
     1561 cccatcgggg accgcgatct tgccgatgtc gtgcagaagg ctgcccagac ggatcgtctc
     1621 ggctatctcg cgtacccctt ccaaccggga tgccaggaga tggcagtact cgctcatccg
     1681 ctggttgtgg gctccggttt cggtgtcgcg tagctccgcg gccagagcaa gtctccggat
     1741 cgtttcttcc tgcgaatctt gcgcctccgc cagtgcgtgc tcgagcttca gcgaggtttc
     1801 ccacaagtcc gatgtcctcg acttgatcat ctcttcgagc ttgtcgcggt gctgctggtt
     1861 ctcgagctcg agcgatctcc ggagcaaagc gttgtagacg ttgatgacca cctcgtcggg
     1921 atcgagcggt ttggataggt agccgtaggc accacattcg acgacgcggt gagcgatctc
     1981 acgatctccc ttgcccgtga ccatcatcgt tgcaacggtg ggacgttcac tcgtgaggtg
     2041 gcggatcagt tcgagacccg acgccccggg catgtcgagg tccgtgatga cgagtgcgaa
     2101 ctctttgcgc gacacctgct ccagtgcctc ggtgacgtcg gcggcttgat cgcactgata
     2161 tccatcctgt tgcaggatcc gggcgagaag acgccgggcc ggttcctcgt cgtcaacgac
     2221 tagtagctga tgcgccccat ttgtattcac tgtatgaatc ttcggtggga attgcccctt
     2281 tctgaatggc cttttgtgat gattctgtct acgtagaacg gccgaggtac ttatggacta
     2341 ggcccccttc ccggatgggg gaagcgtctc ccccgcgccg cttttaaaga ggctgcttgc
     2401 cctctcgtag acgcccacgc gcctcgagga agtcttcgtg cgagacggcg ggctcgccct
     2461 cgcccgaggt tcgggccatc gctgcgagag ccgcttcctg gcacacggcc ttcaggtccg
     2521 caggtgagaa gccctcagtt gcgtcggcga gctcgccggc tccgcaggat tcagtcgtag
     2581 caccccgaac ctttgggaga tgggaccgat caagatcgtg accgccgcac ttgcgctcgc
     2641 cgccgccctc tccacaactg caacccccgc ggccggctcg gcggccgcgg catcaaacga
     2701 atggctcgat cggcgcgtcc tcaacatcgc gcaccagggc ggcgagatcg aggctccctc
     2761 caacacgctg ttcgctttca agacatctct acagaagggg tccgacgtcc tggagctcga
     2821 cgtgcacgcg acctccgacc gagagctcgt ggtgatccac gacgcaacgg tcgaccggac
     2881 cacgaatgga accggccggg tcgaccagtt gacgctggac cagatcaagg agttggacgc
     2941 agcgcactgg ttcgtacccg actgcggcac ctgtcacgac gagccgggcg aggcttacgc
     3001 ctaccgcggc ttcgccaccg gcgagcgtcc catgcgcggc cgcgttgctc gttacgaacc
     3061 aaacgacttc acgatcccga cgctccgcga ggtgctcgag accttcccgg acacgttgat
     3121 caacatcgag atcaaggcca ctgcgcccga cacgatccct tacgagaacg agctcgccga
     3181 tctcttgcgc gagttcgagc gagactcaga cacgatcgtt gtgtccttcc tcgatcacgc
     3241 ggtcgagctg ttcgacgctc tagcaccgga agtcgatacc gcgacgggga ccgctcaagc
     3301 cggtgcgttc tgggcctcgg ggcagggtcc gctccccgga tcgcccagcc ggcaccaggc
     3361 tctgcaggtt ccgatcgaat tcaacggcct gaccgtcgta acggaagagt tcgtcgccga
     3421 agcccacgcc aacggcctgg ccgtccacgt ttggacgatc aacgatcaag agacgatgga
     3481 atggctcatc gagatcggtg tcgacgggat catgaccgac cggcccgaac tgctcgaaga
     3541 cgtgctcaaa gcccagaagg tccgcatcta gcttcggatc tcgatcccac gcggtagctt
     3601 cgcggcatgg aactgaccga aggagtcttt cggctcggca gcccctacgt gaacttctac
     3661 gcgatcgaag agggcggccg gctgacgatc gtggacgggg gcgcgtccgg ctactgggac
     3721 gagataccga ggctcctggc ggcaacgaag ctagcgatca cggacatcgc cgcggtggtt
     3781 ctcacccatt cacacggcga ccacaccggc tgcgtcgaac ggttgcaggc cgagagcggg
     3841 gccgacgtgt tcattcacac cgccgaagaa gcggtcctga ccgggcggga gaagcaggcg
     3901 gcgcccagcg gggctgccgg agctctcctt tccctccagg ggctcaagat gatcgcacac
     3961 atgcttacga acggaggcat gaagtccacg accttgagca cggtcgagac ctataccgag
     4021 gacgaggtgc tcgacgttcc cgggaagcca cgcgtggtgt acacgccggg gcacagcaag
     4081 ggtcactcgg ccctgttcgt cgaacagcgg gacgccctct tcaccggcga cgcgctggtt
     4141 accagagacc tcagggggcg gacgggtccc cgcatgatgg acatcaacgt ggaccacgca
     4201 ggagcgcggc gggcgctgga ccggttcgaa gggatcgagg ccgcgcttct gctccccgga
     4261 cacggcgagc cgtggcggga cggcgtcgcg gccgcggtcg aacgcgcgcg acgcaactaa
     4321 tcagttcgcg atcgagtgga ccgaaggaat gcgcgcactc tttttcgggt gggttcgggt
     4381 aggtgtctgg atgccgagcg cttcgcccgt tcggcgagga cgacccccca tgcccccggc
     4441 cgacccagtt caaggacgtc ccaccactcg agcgtgtcgt gttcgtcggt gaaacgaagc
     4501 ttgtagggat cgtctcccga gccgagatcg aagatcttgt ccccaaccga ccaggaatgc
     4561 tccaacgccg ccatgagagc ctgcatcccg gggcgcatct ctgaccagcc gtcgtcgaaa
     4621 ccacccaacc agtaggacgc cacgtcgccg gccgttagga acatgtggga gctgatcgtg
     4681 tcacccgcta cgtcgatgtt ccacagccgc atcgcaccca ccgggatcaa ccgacgggcc
     4741 gcgctgagaa ccatcgcctc taccgtgctt gacagcgtcg ccgagcctcc cctcgtcgcc
     4801 caccttgctc gatggagctt cgagaacgag cgaacatccg actcgatcga gtcctccgtc
     4861 gtcatgcgaa cactggcccc cgccttctcg agcttccgac ggtcccttct catctgctgg
     4921 cggaaattgg cgctgcgaga tgcgaaccaa tcgtcgaacg tcccctcgcc gagagtgatg
     4981 atcggagcct gcatccacca gtgtttgtgc acgcgatgcg agagcgaccg gccgaagatg
     5041 ggcccccacg gcggtgcggc cttgacgcct tcgagcgtga gtcgcgggat accttgtgaa
     5101 ctaagcacag ccgagatcgt tgcagcggtt tgtcgctcga aggcggggag ggccaaggga
     5161 ccaagctggt tcgaaaccgg ggcagcaagc aggcgggcac gggaaacccg gccccccgtg
     5221 acgaagaagg gcccgatgcc ggccagccgg tcctcatcca tgacggtgat cacgcataag
     5281 cgggatccgg ccgggcggca atgctcccac caggtcaaca tcc
//
LOCUS       DATMED010000122         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5459618, whole genome shotgun sequence.
ACCESSION   DATMED010000122 DATMED010000000
VERSION     DATMED010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5459618"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VNP73_03080"
     CDS             complement(<1..598)
                     /locus_tag="VNP73_03080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL64935.1"
                     /translation="MNFTFNDEQEELRRVVRRFFDERSPSTEVRRLMDTPEGYDDVVW
                     KQMSQDLALQGIHVPEAFGGQGFSFVELGIVLEEMGRALVCAPYFSTVCLGINAILNA
                     ATDEQKAEILPALAAGERTATLAIAEESGKWGADGIELSTDGGGTSYRLSGVKTFVLD
                     GHTADLIVVAARSGSELGLFLVDGHADGLDRQPLKPIDM"
     gene            complement(608..1762)
                     /locus_tag="VNP73_03085"
     CDS             complement(608..1762)
                     /locus_tag="VNP73_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWL64936.1"
                     /translation="MAERCAVLGIGQTKHDGEREDVSIAGLVREAAIRALEDAQLDWA
                     DIDAVVIGKAPDMFEGVVMPELYLADALGATGKPMLRVHTAGSVGGSTAVVASHLVQG
                     GVHQRVLTVAFEKQSESDVMWALAPKIPFSPPIVAGAGGYFAPHIREYIRRSSAPDTV
                     GIKVAVKDRLNALKNPYAHLHMEDISFEQVADSPMLWDPIRYLETCPSSDGACAMVLG
                     SESVAAKSRNPAWVLGTAVRSEPIGMAGRDEVDPRAGRMCAADVYKQAGITNPHRDID
                     VAEIYVPFSWYEPMWLENLGFAEQNAGWKLVDDGTTALDGDLPINPSGGVLSTNPIGA
                     SGMLRFAEAALQVRGEAGEHQVDGAKVALGHAYGGGSQFSAMWVVSSEKS"
     gene            complement(1762..2817)
                     /locus_tag="VNP73_03090"
     CDS             complement(1762..2817)
                     /locus_tag="VNP73_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWL64937.1"
                     /translation="MRDVAVISFAQSASVRREPNLNEVEMIVPVVTEAIENSGIARKD
                     IGFTVSGSCDYLAGQPFAFVMGLDAIGAWPPIQESHVEMDGAWALYEAWVRLQHGDID
                     SALIYCFGKSSLGSLRDVLSLQLDPYYLAPLWPDSVSLAALQARAVLENTDYTERDMA
                     EVAVRSRRDAQDNPNAHVTGDDDPDELLNEPYIAAPLRKHDCPPISDGAAAIVLAAGD
                     LAREKCERPAWIKGIDHRIDAHQLGARDLTKAPSARIAGEAVGAVDNGFDVAEIHAPF
                     THQELILRDEFGLSDSTKLNPSGGALAANPMMVAGLIRIGEAASRVMDGSAGRALAHA
                     TSGPCLQQNLVCVLEGA"
BASE COUNT          546 a          987 c          912 g          539 t
ORIGIN      
        1 tcatgtcgat cggcttcagg ggctgcctgt ctaaaccgtc ggcgtgccca tcgaccagga
       61 ataggccgag ctcggagcct gaacgagcgg cgaccacgat gaggtccgcg gtatgaccgt
      121 ccagcacaaa ggtcttaact ccactcagtc ggtaggaggt gccgccgcca tcggtactca
      181 gttcgatccc gtccgcaccc cacttgcccg actcttcggc gatagcaagt gtcgccgtgc
      241 gctctcccgc cgccagggcg ggaaggatct ccgccttctg ttcgtcagtc gctgcattaa
      301 ggatcgcgtt gatgcccagg caaaccgtcg agaagtacgg agcgcacacg agtgctctgc
      361 ccatctcctc gagcacgatc ccgagctcga cgaagctgaa gccctggccc ccgaaggcct
      421 ccggtacatg gatcccttgc agcgctaggt cctgtgacat ctgcttccaa accacatcgt
      481 cgtatccctc gggtgtgtcc atcagacggc ggacttcggt cgacggcgat ctttcgtcga
      541 agaaccggcg aacgacgcgt cgaagttctt cctgctcgtc gttgaacgtg aaattcacca
      601 gaccccccta actcttctcg ctcgaaacga cccacatggc agagaactga gaaccgccgc
      661 cgtacgcgtg gccgagagcg accttcgcgc cgtcgacctg atgctctccg gcttcgccgc
      721 gcacttgcag agccgcctcg gcgaagcgca acatccccga ggcgccgatc ggattggtcg
      781 agagcacgcc gcccgatggg ttgatcggta gatccccgtc caaagcagtg gtcccgtcat
      841 cgacgagctt ccacccggcg ttctgctcgg cgaagccgag gttctcgagc cacatcggct
      901 cgtaccagga gaaggggacg tagatctctg ccacatcgat gtcacggtgc ggattggtga
      961 tgccggcttg cttgtagacg tcggcggcgc acatgcgccc ggcgcgcggg tcgacctcat
     1021 cgcgaccggc catcccgatc ggctcgcttc ggactgccgt ccccaagacc caagcagggt
     1081 tgcgggactt ggccgcgacc gattccgagc ccagcaccat cgcgcacgca ccatcggagg
     1141 agggacacgt ctcgaggtaa cggatcggat cccacaacat cggggagtcg gccacctgct
     1201 cgaagctgat gtcttccatg tgaaggtgtg cgtacgggtt cttgagggcg ttgagtcgat
     1261 ccttgaccgc gaccttgatg ccgaccgtat cgggagccga cgaacgacgg atgtactccc
     1321 ggatgtgtgg agcgaagtaa ccgccggcac cggcaacgat cggtggtgag aaaggaatct
     1381 tgggagcgag ggcccacata acgtcgctct cggactgctt ctcgaacgcg acggtgagaa
     1441 cacgctgatg gaccccgccc tgaacgagat gagaagcgac gaccgcagtc gatccgccca
     1501 cactgccggc ggtgtgcacg cgcagcatcg gctttcccgt agcacctaag gcatcggcca
     1561 ggtacagctc gggcatcacg acgccctcga acatgtcggg cgccttaccg atcacgacgg
     1621 catcgatgtc ggcccagtcg agctgcgcat cctccagcgc ccggatggca gcttcacgaa
     1681 cgagaccggc gatggacacg tcctcgcgct cgccgtcgtg tttcgtttga cctatgccca
     1741 ggaccgcaca acgctcggcc atcacgcccc ctcgagaacg cagacgaggt tctgttgcag
     1801 gcaagggccc gaggtcgcat gggcgagcgc ccgaccggcg ctcccatcca tcacgcggga
     1861 ggcggcttca ccgatgcgaa ttagcccggc gaccatcatc gggttcgcgg ccagcgcgcc
     1921 tcccgacgga ttcagcttcg tggagtccga tagaccgaac tcatcacgca ggatcagctc
     1981 ctggtgggtg aacggcgcat gaatttccgc aacgtcgaaa ccgttgtcga cggcccccac
     2041 cgcttccccc gcgatcctgg ccgacggcgc tttagttagg tccctggctc cgagctgatg
     2101 cgcatcgatc ctgtgatcga tccctttgat ccacgcgggg cgttcgcact tctcacgagc
     2161 caggtcccca gcggccagca cgatcgcggc cgcaccgtcc gatatgggcg ggcagtcgtg
     2221 cttgcgtagg ggggccgcga tgtagggctc gttcagaagc tcatccggat cgtcgtcgcc
     2281 cgtgacgtgg gcgttcgggt tgtcctgtgc gtcgcggcgg ctgcgcactg caacctccgc
     2341 catgtctcgc tccgtgtagt cggtgttctc tagaacggca cgcgcctgga gtgcggcgag
     2401 gcttaccgag tcgggccaca acggagccag gtaataggga tcgagctgga gcgacagcac
     2461 gtcgcgcaga cttccgagcg acgacttgcc gaagcagtag atgagggcgg agtcgatgtc
     2521 accgtgctgc aagcggaccc acgcctcgta gagggcccac gccccgtcca tctcgacgtg
     2581 cgactcctgg atcgggggcc acgcgccgat agcatcgagt cccatcacga aggcgaacgg
     2641 ttgtccggcg aggtagtcgc aggaccccga gacggtgaag ccgatgtctt tacgtgcaat
     2701 cccagagttc tcgatggctt cggtgaccac agggacgatc atctcgacct cgttgaggtt
     2761 cggctcgcgc cggacactcg ccgactgcgc gaatgaaatg accgcaacgt ctcgcatcag
     2821 tccgcacctt cgatcgcagc gatgactttc tggaacggaa cgtcgggctc tccggtcggc
     2881 ttgaagtact tgatgctctc cagagttggc ttcatctcgg actcgtcgac ccacacggcc
     2941 ttgacccgca tgcccatgcg aacctcttcg gccgcgacct cttg
//
LOCUS       DATMED010000123         6125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4703997, whole genome shotgun sequence.
ACCESSION   DATMED010000123 DATMED010000000
VERSION     DATMED010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4703997"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1305
                     /locus_tag="VNP73_03095"
                     /pseudo
     CDS             31..1305
                     /locus_tag="VNP73_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            1425..3203
                     /gene="dnaX"
                     /locus_tag="VNP73_03100"
     CDS             1425..3203
                     /gene="dnaX"
                     /locus_tag="VNP73_03100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWL64938.1"
                     /translation="MAYLSLYRKYRPQTFSEILGQQHVSRTLANAVDEGRVSHAYLFT
                     GPRGTGKTSTARILAKALNCVDGPTSEPCGKCPSCVAITEGSSLDVFEMDAASHSGVD
                     DTREILSGVPLATAGGRKKVYVIDEVHMLTTQSFNALLKTLEEPPQHVVFVMATTELH
                     RVLPTIVSRTQRFDFRRVPADVIQEHLAHIAKLETIDIESAAVALLARHAEGSVRDAL
                     STLDQLSNFGRTITELDVEGLLGHRNEEALNDVFEAIADSDVGALFSTMQTFVAQGAD
                     MRRLADDVLEHARTLLLLATAPEAEELLDVPVEDRPKLSVQAQRFSPGSLMRVLDLVG
                     QAIIEMRNAPNHRLLLEIALVRSAAPDTDPSATGLLGRIERLERRIGIDSAPEVGGGA
                     ALPQAPPPSKSPSGSTTPPPTRAPQAAATREMPARAKTKPSPPRREDAVGADEHREEV
                     RAAPAASRVAGNVGLAEFKDAWPAVMKEVNKISRRVGALLTPSRAVAHDGDTLTVSLQ
                     AEFHAGEMKKEANRSILAEGLFNVLGVRPALAFTYSGASDQPPEEEVETATDEEDVDA
                     TTAANDDPIELVKQGLGAEVVEERKG"
     gene            3222..3539
                     /locus_tag="VNP73_03105"
     CDS             3222..3539
                     /locus_tag="VNP73_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018218001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWL64939.1"
                     /translation="MQRMMKEAQKMAAEVQKAQAELADVEVEGSAGGGAVKVSATADG
                     RILSVTLDPDLISDSDPDDIEMIGDTIVAAVNDALDKGRAKQEEVMGPIAGGMGGLGL
                     PGM"
     gene            3563..4180
                     /gene="recR"
                     /locus_tag="VNP73_03110"
     CDS             3563..4180
                     /gene="recR"
                     /locus_tag="VNP73_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWL64940.1"
                     /translation="MFAGPIQDLIDELARLPGIGPKSAQRVAFHLLKATPNDARRLAA
                     AIVEAKEKVRLCSECFNIADSELCVYCSDDRRDRQVICVVQEPPDVIAVERTREFHGL
                     YHVLQGAIWPIEGVGPEDLRVGELLDRLQRARNGGAPVTEVILATNPNIEGEATAMYL
                     ARLLEPLGVRVTRPASGLPVGGDLEYADEVTLGRALEGRRSMGGQ"
     gene            4313..5800
                     /locus_tag="VNP73_03115"
     CDS             4313..5800
                     /locus_tag="VNP73_03115"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HWL64941.1"
                     /translation="MRQGHTYFSVKPFFWHAVAATISVIVFPILAVVALLLVVPQLHL
                     LIVALAGVVLSIFAMVVGNTLWSSRVEAGDVSFSDLMVWRWFVRHRAEDTLEEGARLL
                     GLDRSGQPVDDVRIHPEQQLKVLKDLTAALESKDPYTHGHSQRVERHVYRTAAAMHLA
                     LSDIDDLRKAAALHDVGKIRVPDRILRKPDNLTDEERHVIEEHAVVGAWMVSSVGNAD
                     VIAAVRHHHERWDGAGYPDGLTGSEVPLFARIIAVADAYDAITSSRPYRSSSGRDDAC
                     SVLRECAGTQFDPDVVEAFIAALPARLPVTAGLFLILGGGPQRLLRAFASWLKRMGAG
                     SLSPAVGATGAALVLGASVFTPSVPTPRADEVEIAAPAQEQTTDVGALPRTTKRAGSQ
                     EVKVRRQPKQVADLRSDDGAIVLGVRLNRDVDGGEPQPLELDADPTPPKEKPDEPKPD
                     PTHTPTPTKSDEAEPEPAKTNPNDGRDDCPSDGNAGKGNDEDCGD"
     gene            5876..>6125
                     /locus_tag="VNP73_03120"
     CDS             5876..>6125
                     /locus_tag="VNP73_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWL64942.1"
                     /translation="MALIVQKYGGTSIGDADRIRRVAERVVASADQGQKVCVVVSAMG
                     HTTDELVDLAAQISAAPHARELDMLLSAGERISMALLSM"
BASE COUNT         1149 a         1857 c         2065 g         1054 t
ORIGIN      
        1 ccgccgcgct gtttcggcca agaggcaacc atggtcggaa cggccggcga cgacgagctg
       61 gggggaaccg accgatcgga cgtgatcgtg gcgcgcggcg gcaacgacga gatcgaggga
      121 cgtggcggcg acgacctcgt atgcacgggg aagggagccg acttcgtcgt tacgggcaat
      181 ggggctgatc gcatagcgac gggtggtaac ggcgattacg tcgatgccgg ggacggtacg
      241 gatgacgtga ctaccggcaa gggtaaggat cacatccgcg gcggcggcga cgcggacacg
      301 atcgatgccg ggccgcgcgg cgacaggatg atcggcggag gcggggacga ttcgatcgac
      361 ggtggctccg agcttcccaa caatcaagga gatgaggtcg tctacctgag cccgaatggt
      421 cccgtagcgg tcgatctgtc ggccgggacc gcgtccggtg aaggggagga ctctcttacc
      481 gatgtcgagg gggcatgggg aagcggctac tcggattcgt tcgtcggtga tggcggcgac
      541 aactatttct acggcgggtc ctacgcggtc ggcgtcgaag atgactgtgg ctcggacgtc
      601 ttcatcgggg gagacggcaa cgatgtcttg aacgatttcg aatcggaaga ctgcgtcgag
      661 aacgcggatc ggatggaagg tggagccggt tccgattcgc tgtacggcgc atttggcccc
      721 gacgtcctat tgggtggaga cgaccacgac atagttcagg gaggcgacgg agacgacgag
      781 ctggacggcg gcgacaacga tcccaacgaa ggagatctcc tcgaagccgc gtcgggggac
      841 gaccggatcg acgggggacc gggcgagcac gacacggttg cgttcttcaa cgccgaaggc
      901 ggagtcgtgg tcgatctcgc ggcgggtacg gcgaccggca acggatccga cacattgatc
      961 gatctcgagg acgtcgaagg gtctcattcc gcggacacga tcaccgggga cgacggctcg
     1021 aactcgcttc gcggtgacag cgggaacgac tcgatctccg gagccggcgg cgacgatgtc
     1081 attagcggtg actacgacga cgacgttgtg gccggcgatg agggcaatga catggtcacg
     1141 ggagggaccc tgtgcggcag tccattcgac ccgcagtgct ccggagacga ggatccgggt
     1201 aacgaccgac tcgcgggtgg agatggagac gactggctcg ccggcgatcg cggcagtgac
     1261 gagctcgatg ggggccccgg cgtggaccgc ttggatggtg ggcccggcga cgactcatgt
     1321 acgaacggag aggagaacga aggctgtgag tcgggtaggg ggctgctctt ttcgcgttct
     1381 aagcacgagt gggatcgtgg gtgcccccca ctaggatgcg agagatggcg tatctctccc
     1441 tctatcgcaa gtacaggccc cagacctttt ccgagatcct cgggcagcag cacgtgtccc
     1501 ggacgctcgc gaatgcggtc gacgagggcc gtgtatccca cgcctacctg ttcaccggcc
     1561 cccgaggcac cggcaagacc tcaaccgctc ggatcctcgc caaggctctc aactgcgtag
     1621 acggtcccac ttcagaaccc tgtggcaagt gcccttcctg cgtcgctatc accgagggtt
     1681 cttccctgga cgttttcgag atggacgccg cctcgcattc gggcgtcgac gacacccgcg
     1741 agatcttgtc cggcgttcct ctggctacgg cgggaggccg caagaaggtg tacgtcatcg
     1801 acgaggtcca catgctcacg acccagtcgt tcaatgcgtt gctgaagacg ctcgaggaac
     1861 ctccccagca cgtcgtgttc gtgatggcga cgaccgagct ccaccgggtc ctcccgacga
     1921 tcgtctctcg gactcagcgc ttcgatttca gacgcgtgcc ggccgacgtt atccaggaac
     1981 atctggctca catcgcaaag ctcgagacga tcgacatcga gtcggctgcc gtcgctcttc
     2041 tcgctcgtca cgccgagggc agtgtccgag atgctctctc gacgctcgat cagttgtcga
     2101 acttcggtcg gacgatcacc gagctcgacg tcgagggtct tctcgggcat cgcaacgagg
     2161 aggcgctgaa cgatgtcttc gaagcgatcg ccgattccga cgttggtgcg ctcttctcga
     2221 cgatgcagac gttcgttgct cagggcgccg acatgagacg tttggcggac gacgtcctcg
     2281 agcatgcgcg cacactcctc ctactggcga cggctcccga ggctgaggaa ctgctcgacg
     2341 ttcccgtcga agatcggccg aagctgtcgg tgcaagcaca acggttctca cccggttcgc
     2401 tcatgagggt cctcgatctc gtcggccaag cgatcatcga gatgcgcaac gcgcccaacc
     2461 accggctcct gctcgagatc gctctcgttc gctccgccgc ccccgacacc gacccctcgg
     2521 ccaccggcct cctaggccgc atagagaggc tggaacggcg aataggaatc gattccgctc
     2581 cggaggtcgg cggcggcgcc gcactccctc aggctccacc cccttctaag tcgccctccg
     2641 ggagcacaac cccgccgccg acgcgagccc cccaggccgc cgccacacga gaaatgccgg
     2701 cgcgggccaa gacgaagcca agcccaccgc gacgcgagga cgcagtgggt gccgacgagc
     2761 accgcgagga ggtgcgtgct gcgcccgcag cttcgcgagt tgcagggaat gtcgggttgg
     2821 ccgagttcaa ggatgcgtgg cctgcggtga tgaaggaggt caacaagatc tcgcggcggg
     2881 tcggtgcttt gttgacgccg tcgcgggccg tcgctcacga cggggatacg ctcacggttt
     2941 cgcttcaggc cgagttccat gccggagaga tgaagaagga agcgaaccgc tcgatccttg
     3001 ccgagggttt gttcaacgtc ctcggcgtgc gaccggcgct ggcgttcacg tactcgggtg
     3061 cgagcgatca accgcccgag gaagaagtcg aaacggcgac ggacgaagag gatgtggacg
     3121 cgacgaccgc ggccaacgac gatccgatcg agctcgtcaa gcaaggcctc ggtgccgagg
     3181 tcgttgagga acggaaaggc tgagtcaaaa cgatgaaaga catgcaacgc atgatgaagg
     3241 aagcgcagaa gatggccgca gaggtacaga aggcacaggc cgagcttgcc gacgtcgagg
     3301 tagagggaag tgcaggtggt ggggccgtga aggtgtcggc gaccgcagac ggccggatcc
     3361 tcagtgtgac gctcgatccc gacctgatct cggactcgga tcccgatgac atcgagatga
     3421 tcggcgacac catcgttgcc gcggttaacg acgctctcga caagggccgg gccaagcagg
     3481 aagaggtcat gggcccgatc gcgggaggca tggggggcct gggccttccg gggatgtagt
     3541 tgagccgagc cgagaagcac tgatgttcgc cgggccgatc caggatctga tcgacgagct
     3601 cgcccgactg ccgggtatcg gtccgaagag tgcccagcgg gtcgcgttcc atctattgaa
     3661 ggcaactccc aacgacgcca ggcgtttggc cgctgccatc gtcgaagcga aagagaaagt
     3721 ccgactgtgc agcgagtgct tcaacatcgc cgactcggaa ctgtgcgtgt attgcagcga
     3781 cgaccgccgg gaccggcagg tcatctgcgt cgtccaggag cctcccgacg tgatcgcggt
     3841 cgaacggacc cgtgagttcc acggcctgta tcacgtcctc cagggggcga tctggccgat
     3901 cgaaggggtg ggcccagagg atctgagggt cggcgagctt ctcgaccgcc tccaacgggc
     3961 ccgtaacgga ggggctccgg tgaccgaggt gatcctggcg acgaatccga acatcgaagg
     4021 agaggccacg gctatgtacc tcgcccgcct gctcgaaccc ctcggggtgc gggtgacccg
     4081 cccggcgagc ggcctcccgg tggggggcga tctggagtac gcggacgagg tgaccctggg
     4141 ccgagccctg gagggtcgca ggagcatggg ggggcagtaa taaatctttg gctaaggggt
     4201 taagaaaggc acctctggtg ccgatacacg aggaggagga gaatatccgg gcgctgctag
     4261 tatgtacgga gcgtagcggc gcagatacga tcggttagcg gtgggggatt agatgcgaca
     4321 gggccacacc tatttttccg ttaaaccctt tttctggcat gccgtcgcgg ccacgatctc
     4381 cgtcatcgtc tttccgatcc ttgcggtggt tgcccttctc ctcgtagtgc cccaacttca
     4441 cctcctgatc gtcgctcttg ccggagttgt gctgtccatc ttcgcgatgg tggtgggcaa
     4501 caccttgtgg agctcgcgcg tcgaagccgg cgatgtctcg ttctccgatc tgatggtgtg
     4561 gcgctggttc gtccggcatc gcgccgaaga cacgctcgaa gagggcgcgc gcctgctggg
     4621 actggatcgg tctggccagc cggttgatga cgttcggatc catcccgaac agcaactaaa
     4681 ggttcttaaa gatctgacgg cggcgctcga gtccaaggac ccatacacac atggtcactc
     4741 gcaacgcgtc gagcgacacg tgtaccggac cgcagcagcg atgcacttgg ctttgtccga
     4801 catcgatgac cttcggaagg ccgccgcgct gcacgacgtc ggcaagatcc gggtccccga
     4861 tcgcatcctg cgcaagcccg acaatctcac ggatgaagag cgtcacgtta tcgaagagca
     4921 tgcggtcgtc ggcgcgtgga tggtgtccag tgtggggaac gccgatgtca tcgctgccgt
     4981 tcgccatcac cacgaaagat gggacggcgc ggggtatccc gacggcctaa ccggcagcga
     5041 ggtacctctc ttcgctcgga tcatcgccgt ggccgacgcg tacgacgcca tcacttcgtc
     5101 gcggccgtat cgatcctcgt cgggacgtga cgacgcgtgc tcggtgctgc gcgagtgcgc
     5161 cggcacacag ttcgacccag atgttgtcga ggcgttcatc gccgcgcttc ccgcacggct
     5221 gccggtcaca gccggactct tcttgatcct gggtggtgga ccgcagaggt tgctgcgagc
     5281 cttcgccagt tggctcaaga ggatgggagc gggcagtttg tctcccgcgg ttggtgcaac
     5341 cggggccgca ctcgtcctcg gcgcctcagt gttcaccccg agtgttccca caccacgtgc
     5401 cgacgaagtt gagatagccg ctccggcgca ggagcagacg accgatgtag gtgcgttgcc
     5461 tcgcaccacg aaacgagccg gatcccaaga ggtgaaggtg cggcggcagc cgaagcaggt
     5521 tgcggacctg cgtagtgacg atggagcgat cgtgctcggg gtccgtttga accgggatgt
     5581 cgatggcggc gagccccaac ccctcgagct cgatgcggat cccaccccgc cgaaggaaaa
     5641 gccggacgag ccgaagcccg acccgacgca cacgcctacg ccgaccaagt cggatgaggc
     5701 cgaacccgag cccgccaaga cgaatcccaa cgacgggcgg gacgattgtc cgagcgacgg
     5761 gaatgccggc aagggtaacg acgaggactg cggcgactag cttctaggcc tctcggctct
     5821 cagttccacc cctcagcgtc acgtccctag accacctacc cattaccttg ttcttatggc
     5881 cttgatcgtt cagaagtacg gcggaacctc catcggcgac gcggatcgca tcagacgcgt
     5941 tgccgagcgg gtcgtcgcgt ccgcagacca gggccagaag gtctgcgttg tcgtctctgc
     6001 tatggggcac acgaccgacg agttggtcga tctcgccgct cagatatcgg ccgctcctca
     6061 cgctcgcgag ctggacatgc tgttgtcggc cggagagcgg atctcgatgg cgctgttgtc
     6121 gatgg
//
LOCUS       DATMED010000124         5948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5087148, whole genome shotgun sequence.
ACCESSION   DATMED010000124 DATMED010000000
VERSION     DATMED010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5087148"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VNP73_03125"
     CDS             <1..457
                     /locus_tag="VNP73_03125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWL64943.1"
                     /translation="GPGLSDEEADRYLDEMSQIAVLLGADPDAVPRTKSALGDYLRSR
                     ELTATPPAKEAMRFVLYPPVPWPEGRFPEIPLGRLAEIPGRAAYSVLSLATIAILPAR
                     VRRAYRLPWVPVTPPLKASVFAVTRAMSIVMPPPPRMKAALEQHRLTAA"
     gene            540..896
                     /locus_tag="VNP73_03130"
     CDS             540..896
                     /locus_tag="VNP73_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWL64944.1"
                     /translation="MTEDAAAAQRELSRGTVELAVLTLIKTKPRYGYDLLTSLGEATG
                     GGVSIKEGTLYPVLHRLEDAGHIEATWQAEGRSAPRKYYGITPQGRERLALLKTEWER
                     LATGMDRLLNGKGGPK"
     gene            893..1549
                     /locus_tag="VNP73_03135"
     CDS             893..1549
                     /locus_tag="VNP73_03135"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWL64945.1"
                     /translation="MTEEYLKEFERWVAGPKERKKRVRAELEQHLRAAEAAGDVEAMA
                     RLGTPREAAATFSAGHELEPAPLPRRIGAALVDLGIVFLPLIALAIGGLIVDPPGAIE
                     SQFTNVGGLEWMTIVFFFGAGAWWTLGLILSEWRTGRTPGKALLGLRVVTEDGISPSF
                     GQIVIRRLTMIFSGPLQVIDWAFMFFTKKHQRAFELVAKTVVVQDETTTERLEVAAAL
                     "
     gene            complement(1546..3168)
                     /locus_tag="VNP73_03140"
     CDS             complement(1546..3168)
                     /locus_tag="VNP73_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWL64946.1"
                     /translation="MQGLMQDYPLTTNHIFERAERLYPDKEIVTATATGIDRTTYKQW
                     AERTRLLGGALDVLECSADARIGTFAWNTGRHLELYFGIPGTGRVMHTLNIRLFKEQL
                     VYTINHAEDEVIFIDRSLIGLLWPIVSELPKVRNYVVMDDGTGDVPDDKRILDYEQLL
                     TESKPVDLVVDDENRAAAMCYTSGTTGNPKGVVYSHRSTFLHSLMVLASSTLGLNESD
                     RVMPIVPMFHANAWGLPHAGVFAGADFVFPGPSLAPDHIVNLIEKEKVTFTAGVPTIW
                     MGVLPLVDGRDLSSLRLITAGGSAVPKSLSEAFRAKIGLPLLQGWGMTETSPVASLGN
                     IKSSLANESDEKKAEFRAHAGLPVPMCDIRIVAEDSGEELAWDGEVFGELQIRGPYIA
                     SAYYNDPTSDEKFTPDGWLRTGDVASVTPEGYIKIVDRTKDVIKSGGEWVSSVELENE
                     IMAHPKVAEAAVIGVPHPKWMERPIACVVPKEGEDVTKEEILEFLKPRVAKWWLPEDV
                     QFIEEIPKTSVGKFSKKDLRQRFADYKLPETT"
     gene            3270..5900
                     /locus_tag="VNP73_03145"
     CDS             3270..5900
                     /locus_tag="VNP73_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64947.1"
                     /translation="MSLPANPIWRKTPFLLFRWPPILAAVLASFVVLVVAASVGPLFT
                     SASAAGALDRQLVGITPAAAGLTVLQETPLEPVMLQSPGGEPQEVSPIELHAFRRRGL
                     DRALEPIDHLGPPISSMVLGASTLASPEGELAVRPTSRDGFVDHVEIVTEVEGDGLWV
                     PDNIAEALAIEAGDEVELTVDAPTAIRVKGIYRDLTLVPASAYWRPVDRYIHPQRPQD
                     ADPPALLLGDPEDLIEIGTKTETFAVAIWEYPIVRDGLTFEEARTIRRQLSAVSGDAL
                     DTSTDVGFYLNEPEVQTVLPIVVDTAAEVVAGIRLPVQVLVLTGAGVALLLVAISAHY
                     MVQKRAVEFRLLESRGVSHVFIGLRAIAEAVVPVVLATAMGSVGTWLMLKAFGPGPVD
                     PSALADSARFTAACVVIGLAALGIGTALVARRHMSVEHSAARVGAGRPWEISLLVAAG
                     AALFTIRERGGVGQGDGSVGDLLLVVWPILAVAGAALLVLKLGLGYLRRGLRVTEGSG
                     TALFLATRRLVAAARSVALLIGAAAVSVGILTYSTALLASVRTTTHAKTHVFTGSDVS
                     LQVSNLDLPDVNAPHTVVGTTAADADGEEVTLMGIDPDTFAAGAYWDETFANDPLSSL
                     VERVASTTAEPLPVIVAGSQADVDVITVETTGAAVEIDVVGRARTWPGMVQDTPMIIG
                     DLTSIDDIEGVSFETLNLWAKGPIEVVFERLRQAGFDTDFAITTDDVGRSSRLLSISW
                     TFGLLRVLGLVAGSLAVVGMLLYVASRRRTRLVSYLLGRRMGLTPSVHMRSLAIELLV
                     MLMSALILGALFGIVSARLVYPELDLLPEVPPEPLFRLPVTEVVATAIGFVLAGVIGA
                     ALIQRSGDRARPGEVLRLAD"
BASE COUNT          978 a         1906 c         1917 g         1147 t
ORIGIN      
        1 cgggcccggg ctctccgatg aagaagccga ccgctacctg gatgagatgt cacagatcgc
       61 cgttctgctc ggagccgacc ccgatgccgt gccccgtacc aagtctgcgc tcggggatta
      121 tctgcgctcg cgagaactga ccgcgacgcc tccggcgaag gaagcgatga ggttcgtcct
      181 ctatccaccc gtcccatggc ctgaaggccg gttccccgag atcccgctgg ggcgtctcgc
      241 cgagatcccc ggccgggcgg cctactccgt gctgtcgctg gcgacgatcg cgatccttcc
      301 cgcgagggtc cgtcgtgcct accgcttgcc ctgggttccg gtgactcccc cgctaaaagc
      361 cagtgtgttc gcggtcactc gggcgatgtc gatcgtgatg cctccgcctc ctcgcatgaa
      421 agctgcgctc gagcagcacc gactgaccgc tgcgtaaagc cgtccgacaa aggtcttgac
      481 cgaccctgct cgaccatgta ccttgtgaca caaggtagct tggaccacaa ggacaaatca
      541 tgacggaaga cgcagcagca gcacagaggg aactgtcacg cggcacggtc gagctcgcgg
      601 tgctgacgct gatcaagacg aagcctcgct acggctacga cctcttgacg agcctcggtg
      661 aggcgaccgg aggtggcgtc tcgatcaagg aagggacgct ctaccccgta cttcatcgcc
      721 tggaggacgc ggggcacatc gaggccacgt ggcaagcgga ggggcgctcg gccccccgca
      781 agtactacgg catcacgcca caggggcggg aacgactcgc cttactgaag acggaatggg
      841 aacggctcgc aaccgggatg gaccggctgc tcaacggaaa gggaggaccg aagtgaccga
      901 ggagtacctg aaggagttcg agcgttgggt cgcgggaccc aaggagcgca agaagcgcgt
      961 ccgcgccgaa ctggaacagc acttacgcgc cgccgaggcg gccggcgatg tcgaagcgat
     1021 ggctcgtctc ggcacgccgc gagaggcggc tgccactttc tcggccggtc acgagttgga
     1081 gcccgctccg ctcccccgcc ggatcggtgc cgcgctcgtc gacctcggca tcgtcttctt
     1141 gccgctgatc gcgcttgcca tcggtggctt gatagtcgat ccgcccgggg cgatcgagag
     1201 ccaattcacg aacgtcggcg ggctcgagtg gatgacgatc gtgttcttct tcggtgcggg
     1261 ggcgtggtgg accctcgggc tcatcttgtc cgagtggcgc accggacgga ccccgggcaa
     1321 ggccctactc gggctgcgcg tcgtgaccga agacggcatc tcccccagct tcggtcagat
     1381 cgtcatccgc cggctcacga tgatcttctc gggtcctctg caggtcatcg actgggcgtt
     1441 catgttcttc accaagaaac accagcgtgc gttcgagctc gtggcgaaga cggtcgtggt
     1501 ccaagacgag accacgaccg aacgcctcga ggttgctgct gcgctctagg tcgtctcggg
     1561 caacttgtag tcggcgaagc gctgacgcag atccttcttc gagaacttgc ctaccgaagt
     1621 tttcgggatc tcctcgatga actgaacgtc ctctggcagc caccacttgg cgacccgagg
     1681 cttgaggaac tcgaggatct cttccttggt gacgtcctct ccttccttcg ggacgacgca
     1741 tgcaatagga cgttccatcc acttcgggtg tggaacgccg atgaccgcag cttcggcaac
     1801 cttggggtga gccatgatct cgttctcgag ctcgaccgac gagacccact cccctcccga
     1861 cttgatcacg tccttcgtgc ggtcgacgat cttgatgtag ccctccggag ttacggacgc
     1921 gacgtcgccg gttcgtagcc agccatccgg cgtgaacttc tcgtcggagg tcgggtcgtt
     1981 gtaataagcg gacgcgatgt aggggccgcg gatctgaagc tcgccgaata cctcgccgtc
     2041 ccacgcgagc tcttctcccg agtcctcggc gacgatcctg atgtcgcaca tcggaacggg
     2101 gaggcccgcg tgcgcgcgga actctgcttt cttctcgtca gactcattcg cgagactcga
     2161 tttgatattg cccagcgaag cgaccggact cgtctccgtc atcccccatc cctgcagcaa
     2221 gggaaggccg atcttggccc ggaacgcctc actcagagac ttgggtacgg ccgaaccacc
     2281 ggcggtgatc agtcgaaggc tcgacaggtc gcggccatcg accagcggca gcacgcccat
     2341 ccagatcgtc ggcaccccgg cggtgaacgt gaccttctcc ttctcgatca ggttcacgat
     2401 gtggtccgga gcaaggctcg ggccggggaa gacgaaatcg gcgccggcga acacgccggc
     2461 gtgcgggagc ccccatgcat tcgcgtggaa catcggcacg atcggcatga cgcgatccga
     2521 ttcgttcaag ccgagggtcg aagacgccag caccatcaac gagtgcaaga acgtcgaacg
     2581 gtgtgagtac acgacgccct tcggattccc cgttgtgccc gacgtgtagc acatggccgc
     2641 ggcgcggttc tcgtcgtcga cgacgaggtc gaccggcttc gactcggtca gaagctgctc
     2701 gtaatcgagg atccgcttgt cgtcgggaac gtcgcccgtc ccgtcgtcca taacgacgta
     2761 gttgcgtacc tttggaagct ccgacacgat cggccacagg aggccgatca gagatcgatc
     2821 gatgaaaatg acctcatcct cggcgtgatt gatcgtgtag acgagctgtt ccttgaacaa
     2881 gcggatgttc agggtgtgca tcacgcgccc ggtgcccggg atgccgaagt agagctcgag
     2941 gtgccggccc gtgttccacg cgaacgtccc gatgcgagcg tcggccgaac attcgagaac
     3001 gtcgagcgca ccgccgagca atctggtccg ttcggcccac tgcttatagg tcgtccggtc
     3061 gatccccgtc gcggtggcgg tcacgatctc cttgtcggga tacagacgct ccgcgcgctc
     3121 gaagatgtgg ttggtcgtga gtgggtagtc ctgcatcaaa ccctgcatgc catgccccct
     3181 tcttcctgct tgtcttgtcc cgcgactccg agtgtaggaa atcccagcca gcccccggcc
     3241 actactgctg tagtagggtc gctcggcccg tgagcctgcc tgccaatccc atctggcgca
     3301 agaccccgtt cttgctgttc cgctggccgc cgatcctggc ggctgtgctc gcgagcttcg
     3361 ttgtgttggt cgtcgcggcg tcggtagggc cactgttcac ctcagcctcc gcggcaggtg
     3421 ctttggatcg gcagctcgtc gggatcactc ccgcggcggc gggactgacc gtccttcaag
     3481 agacgccgct cgagccggtg atgctgcagt ctccgggcgg cgaaccgcaa gaggtctccc
     3541 ctatcgaatt gcatgccttt cggagacgag gactcgaccg cgctcttgag ccgatcgatc
     3601 acctcgggcc ccccatctcg tcgatggtgt tgggcgcctc gacgcttgcc tccccggagg
     3661 gggagctggc ggtgagaccg acgtcgcgcg acgggttcgt cgatcacgtc gagatcgtca
     3721 cggaggtcga gggagatggg ctctgggtcc ccgacaacat cgccgaagcc ctggccatcg
     3781 aagcgggcga tgaggtcgag ttgacggtcg atgctcccac tgcgatccgg gttaagggca
     3841 tctacagaga cctcaccctg gtaccggcga gtgcgtactg gcgaccggtc gaccgttaca
     3901 tccatcccca gcgaccgcag gatgccgacc cgcccgccct cttactggga gacccagagg
     3961 acctcatcga gatcggaacc aagaccgaga cgttcgccgt cgcgatctgg gagtacccga
     4021 tcgtcaggga cgggttgacg ttcgaggagg cgcgcacgat cagacggcag ctgagtgcgg
     4081 tgtcgggaga cgctctcgac acgagcacgg acgttggctt ctatctgaac gaaccagagg
     4141 ttcaaacggt gttgccgatc gtcgtcgaca ccgccgccga ggtcgttgcg ggcatccgct
     4201 tgcctgtgca ggtactcgtg ctgacgggtg cgggggtcgc gctgcttctc gttgctatct
     4261 ccgcgcacta catggtgcag aagcgagcag tcgagttccg cttgctcgag tcgcgtgggg
     4321 tatcgcacgt cttcatcggc cttcgggcta tcgcggaggc ggtagtgccg gtcgtgctcg
     4381 ccaccgccat ggggtcggtc ggaacgtggc tgatgttgaa ggccttcggt ccggggccgg
     4441 tcgatccatc tgcgctcgcg gactcagcgc ggttcaccgc cgcctgcgtg gtgatcgggc
     4501 tggcggcgct ggggatcgga accgcgctcg tcgctagacg tcatatgtca gtcgagcatt
     4561 cggcagcgcg ggtgggagcc ggacggccgt gggagatctc tctcctggtt gcagccggcg
     4621 cggcactctt tacgatccga gagcgcggcg gtgtaggtca gggcgacggc tccgttggcg
     4681 acttgttgtt ggtggtgtgg ccgatccttg cggtagccgg cgccgctctg ctcgtcctca
     4741 aactcggcct cggttatctg cgccgaggtc tgcgcgtcac ggaaggttcc ggcaccgcct
     4801 tattcctcgc gacgcgccgg ctcgttgctg ccgctcgatc ggtcgcactt ctgatcgggg
     4861 ctgcggcggt gtcggtcggc atcctgacct actcgacggc tctcctcgcg tccgtgcgca
     4921 ccacgactca cgctaagacc cacgtcttca cgggatctga tgtgtcgcta caggtttcga
     4981 atctcgatct gcccgacgtc aacgctcccc acacagtcgt tggaacgacg gccgcagatg
     5041 ccgacgggga agaggtgacg ttgatgggta tcgaccctga cacatttgcg gctggtgcct
     5101 actgggacga gaccttcgcg aacgatccgc tgtcctcgct cgtggaacgg gttgcctcca
     5161 ctactgcgga gccgttgccg gtgatcgttg ccggatcgca agcggacgtc gatgtgatca
     5221 cggtcgagac gaccggggct gccgtcgaga tcgatgtcgt gggccgcgcc cgcacctggc
     5281 ccggcatggt tcaggacacg ccgatgatca tcggggatct cacgagcatc gacgacatcg
     5341 aaggcgtgtc cttcgaaacg ctgaacctgt gggcgaaagg accgatcgag gtcgtcttcg
     5401 aacggctgcg tcaagccggc ttcgacacgg attttgcgat caccaccgac gacgtcggcc
     5461 gctcctcgcg cctgctgtcg atctcgtgga cattcggcct tttgcgggtg ctcggcctcg
     5521 tcgccggatc gctcgcggtc gtggggatgc tcctctatgt cgcgtcccgg cgacggacga
     5581 gacttgtgtc ctacctactc gggcgacgta tgggtttgac accaagcgtc catatgcggt
     5641 cgcttgcgat cgagctcctc gtcatgctca tgagcgcgct gatcctcggc gcgctctttg
     5701 ggatcgtctc ggcgcggctc gtctatccgg aactcgatct actgccggag gttccgccgg
     5761 agccgctctt ccgattgccg gtaacggagg tcgtggccac cgcgatcggg ttcgtgctgg
     5821 ccggggtgat cggcgccgct ctgatccagc gatccggtga ccgcgcccgg cccggggagg
     5881 tgcttcgcct tgcggactga agccgtcgcc gagtgccgtg agctgagccg aaccttcacg
     5941 accggatc
//
LOCUS       DATMED010000125         4184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5461167, whole genome shotgun sequence.
ACCESSION   DATMED010000125 DATMED010000000
VERSION     DATMED010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5461167"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VNP73_03150"
     CDS             <1..441
                     /locus_tag="VNP73_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64948.1"
                     /translation="IEADLDFSLIRLDAGIQPQAQVAHWGGPTRINQEITYEPQLLKM
                     FGSGLVVEDAVPQRQLVAPNGLDGEEDLYVTGVATPGDSGAPALTDSGEALGVVVSLS
                     PTLRIPEQGPPYVGVVRIARLPFNLAIAEKLLGIDLKIERAPVL"
     gene            complement(438..1823)
                     /locus_tag="VNP73_03155"
     CDS             complement(438..1823)
                     /locus_tag="VNP73_03155"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HWL64949.1"
                     /translation="MTTFDSLLGGIGNTPLLRLQKLGRGLGCDLLGKLEMMNPGGSVK
                     DRIGLPMIEAAEREGKLKPGGTIVEPTSGNTGVGLAIAAAIKGYRCIFTMPDKMSQEK
                     IALLRAYGAEVVITPTMVPPESPESYYRVADRLTEQIPGAFQPNQYHNDNNPQAHYDT
                     TGPEVWEQTEGRITHFVVSVGTGGTVTGAGRFLKEKNPDIVVVGADPEGSTFTGEVHP
                     YLVEGIGKESWPDTLDPSVIDRWVRVSDRDSFLTARRITREEGLLVGGSSGTAAWAAL
                     EVARELDDSAVVVVILPDSGKSYLSKIYNENWMLEHGFIERPGTSARIGEIIDDKQSH
                     GPAIPDLVAVPTGAKVGGAIDLLQQYGISQMPVAKTDQPHDVSEIVGSIQERSLLDRV
                     FRDRDAVGRDVVEVMDPPLPVVQTSAGVEEMFADLARGAEAVLVAEGGTPIGVLTRAD
                     LLEFLSHQARP"
     gene            1900..3123
                     /locus_tag="VNP73_03160"
     CDS             1900..3123
                     /locus_tag="VNP73_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HWL64950.1"
                     /translation="MRFSVMRRFGLPGEQLRRWIFAALFVIGTVASATVAYATYDYSK
                     QYRGKILPGTSVAGVDLGGMTLDDAKVAVRRAIEPELRQTIVFRYKERTWRVSARQLG
                     ARGDVTETVSDAAFASREASFWDKARLRMLGSHGWFDRPLAISYPRRALHGFLEGIAA
                     GFDRPVRDATVDYSTGWVKVVPEQEGRELMRKETYRRLTRALDVGLTEVRLAVDAQKA
                     KVKEDEFDQVLLLRIGENKLYLYEDGKIAHKWTVATGQPEYPTPTGIYEVTEKRYMPT
                     WVNPDPEGWGASMPLSIPPGPGNPLGVRAINWSAEAIRFHGTSATYSLGYNASHGCVR
                     MANEEVIKLYDMIDVGTPIVSTIVAPLKPMYVSAPDPTVVEEDAPTDGSSEADEDNPP
                     REEGRKGGSERKKDR"
     gene            3123..3923
                     /locus_tag="VNP73_03165"
     CDS             3123..3923
                     /locus_tag="VNP73_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HWL64951.1"
                     /translation="MAQTPRPPRFGEQDRRLSYGSYLKVPEILSHQELLSDPPAHDEL
                     LFIVVHQVYELWFKQLLFELESIRELLFQEDTVRARHLLHRVHTIESVLIEQIPVLES
                     MSPQDFLEFRLHLAPASGFQSVQFREIEFISGLKDGSYLDRLADSGEAKERLVRRLAE
                     PTIWDAFCSLLTQGGLPMPAEDEDTRRASLLTMFSERDKYAEQFDVSEAMLTHDEHFA
                     LWRQRHILMVERQIGTKTGTGGSTGASYLRTTLEKRFYPELWDLRSFL"
BASE COUNT          777 a         1328 c         1315 g          764 t
ORIGIN      
        1 atcgaagcgg atctcgactt ctccttgatc aggctggacg cgggcatcca acctcaagcc
       61 caagtcgcac actggggagg tcccacgcgc atcaaccaag agatcacgta cgagcctcaa
      121 ctactgaaga tgttcggtag cggcctggtg gtcgaggacg cggtcccgca acggcagctc
      181 gttgcaccca atggcctcga cggtgaagag gacctgtacg tcaccggggt tgcgaccccc
      241 ggagattcgg gagcgcccgc gttgaccgac tcgggtgagg ctctcggtgt tgtcgtgagc
      301 ctgagcccga ccctgcgcat ccccgagcag ggcccgcctt acgtgggtgt cgtgcggatc
      361 gcgcgtttgc ctttcaacct cgccatcgcg gagaagctgc tgggtatcga tctcaagatc
      421 gagcgggcgc ctgtcctcta gggccgagct tggtgcgaga ggaactcgag taggtccgcc
      481 ctcgtaagga ccccgatcgg tgtgccgccc tccgcaacga gaacggcctc ggccccccta
      541 gcgagatcgg cgaacatctc ttcgaccccg gccgacgtct gaacgaccgg taggggcgga
      601 tccatcacct ctacgacgtc gcggccgacc gcatcccggt cacggaagac gcgatcgagc
      661 aggctgcgct cctggatcga gccgacgatc tcggacacgt cgtggggttg atcggtcttg
      721 gcgacgggca tctgcgagat cccgtactgc tgcagcaagt cgatcgctcc gcccaccttg
      781 gccccagtgg gaacggccac gaggtccggg atggcggggc cgtggctctg cttgtcgtcg
      841 atgatctcgc cgatccgagc agacgtcccc ggacgctcga tgaagccgtg ttccagcatc
      901 cagttctcgt tgtagatctt cgaaaggtag ctcttgcccg agtcgggaag gatgacgacc
      961 accaccgccg agtcgtccag ctcccgcgct acttccagcg cggcccacgc cgccgttccc
     1021 gacgaaccgc cgaccagcaa cccctcttct ctcgtaattc gccgagcggt caagaacgag
     1081 tcgcgatccg aaacgcgcac ccagcgatcg atgacgctcg gatcgagcgt gtcgggccac
     1141 gactcctttc cgatgccttc gacgagataa gggtgcacct cacccgtgaa cgtcgagcct
     1201 tccgggtcgg ccccgaccac aacgatgtcg gggttctttt ctttgaggaa gcgtcccgcg
     1261 ccggtgacgg ttccgccggt cccgacgctg acgacgaagt gagtgatccg tccttcggtc
     1321 tgctcccata cctccgggcc ggtcgtgtcg tagtgcgcct gcggattgtt gtcgttgtga
     1381 tactggttgg gctggaacgc gccggggatc tgttccgtca agcgatccgc cactcggtag
     1441 taagactcgg gcgactccgg aggaaccatg gtcggtgtga tcacgacctc ggccccatac
     1501 gcgcgcaaca gcgcgatctt ctcctggctc atcttgtcgg gcatcgtgaa gatgcagcga
     1561 taccccttga tcgctgcggc gatcgcgaga cccacaccgg tgttgcccga ggtcggctcg
     1621 acgatcgtcc cgccgggctt cagcttgcct tcccgttccg cggcctcgat catcgggagg
     1681 ccgatccggt ccttgacgct tcccccggga ttcatcatct ccagcttgcc gagcaggtcg
     1741 cagccgagcc cacgcccgag tttctggagg cggaggagtg gcgtgttgcc gatcccgccg
     1801 agcaagctgt cgaatgtggt cattgctcga atcgtttacc aggtgggtgg ctaaggcaag
     1861 gtcccaccta accgagccac ttccccttag catttccgta tgcgtttctc cgttatgcgt
     1921 cgcttcggcc tgcctgggga gcaactccgc cggtggatct tcgcggccct cttcgtgatt
     1981 gggaccgtgg cttcggcgac ggtggcctat gccacctacg actacagcaa gcagtacagg
     2041 ggcaagatcc tgcccggcac gtcggtggcg ggagtggacc tcggcgggat gaccctggac
     2101 gacgcaaagg tcgcagtgag aagggcgatc gagcccgagc tgcgccagac gatcgtcttc
     2161 cgctacaagg agcggacctg gagggtcagc gcccgacagc tcggtgctcg aggggacgtg
     2221 accgagacgg tgtcggatgc cgcgttcgca agccgcgagg cgagcttctg ggacaaggcg
     2281 cgcctccgca tgctgggatc gcacggttgg ttcgatcgac cgttggcgat cagttacccg
     2341 cgccgggcac tgcacggttt cctggaaggg atagcggccg ggttcgatcg tccggtgcgc
     2401 gacgcgaccg tcgactactc gaccggttgg gtcaaggttg ttccggaaca agaaggccgc
     2461 gagctcatgc ggaaggagac ctaccgacgg ctaacgcgcg cattggacgt cggactgacc
     2521 gaagtgcgtc tggccgtcga cgcgcagaag gcgaaagtca aggaggacga gttcgatcag
     2581 gtcctgctgc tgcggatcgg cgagaacaag ctctatctct acgaggacgg gaagatcgcg
     2641 cacaagtgga cggtcgcgac cggccagccg gaatatccga ccccgacggg gatctacgag
     2701 gtcaccgaga agcgctacat gcccacctgg gtcaaccccg accccgaggg ctggggggcg
     2761 agcatgccgc tttcgatccc accgggcccc ggtaaccctc tcggagttcg agcgatcaac
     2821 tggtccgccg aggcgatcag attccacggc acgtcggcga cctactcact cggctacaac
     2881 gcgtctcatg gatgtgtgcg gatggcgaac gaagaagtga tcaagctcta cgacatgatc
     2941 gatgtcggta cgccaatcgt ttcgacgatc gtcgctccct tgaagccgat gtacgtctcg
     3001 gcccccgacc ccacggtcgt ggaagaagac gcgcctaccg acgggagcag cgaggccgac
     3061 gaggacaacc caccccggga agaaggtcgc aagggcggct ccgagcgcaa gaaggatcgc
     3121 tgatcgcgca aacgccgcgg ccgccgcgct tcggggagca ggatcggcga ctttcctacg
     3181 ggagctacct gaaggttccc gagatcctgt cgcatcaaga gctgttgtcg gatccgccgg
     3241 cccacgacga gcttctcttc atcgtcgttc accaggtcta cgagctctgg ttcaagcagt
     3301 tgctcttcga gctcgaaagc atccgcgagc tgctgttcca agaggacacc gtgcgtgctc
     3361 gccaccttct ccaccgcgtg cacacgatcg aaagtgtctt gatcgagcag atcccggtcc
     3421 tcgagtccat gtccccgcag gacttcctgg agtttcggct ccacctggcc ccggccagcg
     3481 ggttccagtc ggttcagttc cgggagatcg agttcatctc cggtctcaag gacgggtctt
     3541 acctcgaccg cttggcggat tcgggcgagg cgaaggaacg cctggtgcgc cggctcgccg
     3601 agcccacgat ctgggacgcg ttctgctccc tcctcacgca gggcggcttg ccgatgccgg
     3661 cagaggacga ggacacccgg cgcgcgagtc tcctcacgat gttctcggag cgagataagt
     3721 acgcggaaca gttcgacgtg tccgaggcga tgctcacgca cgacgagcac ttcgcgctat
     3781 ggcgccagcg tcacatcttg atggtcgagc gacagatcgg gacgaagacg gggaccggtg
     3841 gctcgaccgg agcgtcctat ttgcgaacca ctttagagaa acgcttctac cccgagctgt
     3901 gggatctgcg cagcttcctt taagcgcgaa gaaacgtttg acgaacgcga agaaaaatcg
     3961 gcgcaacctc ttgtggtcgc gaaactaggt gttttaaact gtttgagtac ctcgcagagg
     4021 taagtaggtc tcggccgaaa gccactgcga ggtgcatttt tttttggcac cgaacgggtt
     4081 gactcacctg cgtcccttca atcaacagga cgcaaccggc gaacagcaaa gaagtgctga
     4141 accagtagaa agaccaagag cccgacaccc acccagcgaa tcgc
//
LOCUS       DATMED010000126         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2406730, whole genome shotgun sequence.
ACCESSION   DATMED010000126 DATMED010000000
VERSION     DATMED010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406730"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VNP73_03170"
     CDS             complement(<1..776)
                     /locus_tag="VNP73_03170"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWL64952.1"
                     /translation="MTQPHLGIAAGSRLGCRAAEKVARSGGNAVDAALASAITAWVAE
                     PFFASLGGSGFVAVRTPDGAVEVIDGNNMMPHTPPEEPGQGMKRIFLPDYADGIYMGI
                     GGGSIAVPGILAAVRTAWERHGRIEWEALFSDAIDVARSGFPFPKTSDYYLSCTWGEI
                     WSKYEGARSLFYVDGRPMREKDTLLQPELAEVLREIATKGPDVFYKGEIGNDIAESIA
                     KDEGFLTLDDLNSFETEIRGSISTTAFGWLIESNPPPSVGG"
     gene            813..1259
                     /locus_tag="VNP73_03175"
     CDS             813..1259
                     /locus_tag="VNP73_03175"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWL64953.1"
                     /translation="MASRSRLRRSFTVESSRVTRHSVDSVLDLLLSPATWPRWQPEIV
                     SVSGPEKLAVGDVVEGRARMLGFDVDGRSTTVGIDAHDFEEDVIVGVRMRVSYRVEPS
                     DQGSIVTRKLVAELPGGFAGRVLSLLLRPRLRAMQKEVLDALARAS"
     gene            complement(1256..1657)
                     /locus_tag="VNP73_03180"
     CDS             complement(1256..1657)
                     /locus_tag="VNP73_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWL64954.1"
                     /translation="MKLLRDVAVRHLLTIEPDASLRRAAQVMTDRGVGCAIVIDNEQI
                     AGIITERDILHSVSSQQNVDETKVREVMTKDVVSGQPGWDLLKAVKTMTDGGFRHLLV
                     LEMNDPVGIVSLRDLMDSMAEMVQTTDSPSS"
     gene            1690..2445
                     /locus_tag="VNP73_03185"
     CDS             1690..2445
                     /locus_tag="VNP73_03185"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HWL64955.1"
                     /translation="MIVGAHVPMEDPLGQAETVGAECVQLFVGPPQSWKKPPPLPDAE
                     RIRSASIPVYVHAPYLINVASPNNRVRIPSRKILADTVSRSEEIEAAGVIVHAGHAED
                     GIERGFVRWRKVFEELETDVPILIENTAGGDNAMGRKVDVLAELWEHLADWDVGFCFD
                     TCHAHAAGEDLGTVVERVLGATGRIDLVHANSSKDPAGSGRDRHCNFSDGELDLDVLL
                     EMIQASGAPACVCETPWEGIAEDVKQVKARIAG"
BASE COUNT          445 a          790 c          823 g          483 t
ORIGIN      
        1 ccgccgaccg agggaggcgg gttcgattcg atcagccacc cgaatgcggt cgtagagatc
       61 gatcctcgga tctcggtttc gaacgagttc agatcatcca gggttaggaa cccttcgtcc
      121 ttggcgatgg actcggcgat gtcgttcccg atctcgcctt tgtagaagac gtccggaccc
      181 ttggtcgcga tctcccggag cacttcggcg agctcgggtt gaagaagtgt gtctttctcc
      241 cgcatcggcc ttccgtcgac gtaaaacaac gacctggccc cctcgtactt ggaccagatc
      301 tcgccccagg tacacgagag gtagtagtcc gacgtcttcg ggaatgggaa acccgaacgc
      361 gcaacatcga tggcatccga gaacaacgcc tcccactcga tgcgcccgtg cctctcccat
      421 gcggtgcgaa ccgcagccag gatgccgggc acggcgatcg acccaccccc gatccccatg
      481 tagataccgt ctgcgtaatc gggaaggaag atccgcttca tgccctgacc gggttcctcg
      541 ggtggcgtgt ggggcatcat gttgttcccg tcgatcacct cgacggctcc atccggggtt
      601 cggacggcca cgaagcctga tccacctaga gaggcgaaga agggctcggc aacccacgct
      661 gtgatcgcgg aggccagggc cgcatcgacc gcgttgcctc ccgagcgagc gaccttctcg
      721 gccgcgcggc agcccaatcg gctgccggct gcgatcccga ggtgtggctg cgtcaccggg
      781 gcaggttaat gcgtcgaacc taggatggcc ccatggcttc gagatcgcga ttgcgccggt
      841 cgttcaccgt tgaatcgagc cgcgtcacga ggcactcggt cgattcggtc ctcgacctgc
      901 tccttagccc ggcgacatgg ccccggtggc agcccgagat cgtgtcggtc tctggccccg
      961 agaaactggc ggttggtgac gtggtcgaag gccgggcccg gatgctcggg ttcgatgtcg
     1021 acggtcgcag cacgaccgta gggatagacg ctcacgattt cgaggaagac gtgatcgtcg
     1081 gagtgaggat gcgggtctcc taccgggttg agccgagcga tcaggggtcg atcgttaccc
     1141 gcaagctcgt cgcggagctg cccggggggt tcgcggggcg cgtcctatcc ctgttattga
     1201 gaccgcgcct cagggcgatg cagaaggaag tgctggacgc tctggcgcgg gcgagctagc
     1261 tcgacgggga gtccgtggtc tggaccatct ccgccatgga gtccatcagg tctctgagcg
     1321 acacgattcc gacggggtcg ttcatctcga gcacgagcaa gtgacggaac ccaccgtcgg
     1381 tcatcgtttt gaccgctttc agaaggtccc aaccgggctg gccggacacg acatctttgg
     1441 tcatgacctc acggaccttc gtctcgtcga cgttctgctg gctcgagacc gagtgaagga
     1501 tgtcgcgctc ggtgatgatc ccggcgatct gctcgttgtc gatcacgatc gcgcatccga
     1561 ccccccggtc ggtcatcacc tgggcggccc ggcgcaggct ggcatccggc tcgatcgtga
     1621 gcaggtgtct tacggctacg tctctcagga gtttcatcgt cgctgcatat tgccatgtag
     1681 cttcgaggaa tgatcgtggg ggctcatgtg ccgatggagg acccgctggg ccaggctgaa
     1741 accgttgggg ccgaatgcgt tcagctcttc gtaggcccac cccagtcctg gaagaaacca
     1801 cctccgctgc ccgacgctga gcggatccgg tcggcgtcga tccccgtcta cgtccatgcc
     1861 ccctatctga tcaacgtggc ttctcccaac aatcgcgtgc ggatcccctc tcgcaagatc
     1921 cttgccgaca cggtgtcgag atccgaagag atcgaggctg cgggggtcat cgtgcatgcc
     1981 ggccatgccg aggacgggat cgagcgggga ttcgtccggt ggcggaaggt cttcgaggaa
     2041 ctcgaaaccg acgtaccgat cctgatcgag aacactgcgg gtggggacaa cgcgatgggt
     2101 cgcaaggtgg acgtcctagc cgagctctgg gagcaccttg ccgactggga cgtcgggttc
     2161 tgcttcgaca cctgtcacgc acacgcggcc ggtgaagatc tgggaaccgt ggtcgagcgc
     2221 gtgctgggcg cgacgggtcg tatcgatctg gtccacgcga atagctcgaa ggaccccgcc
     2281 ggcagcggcc gcgaccgtca ctgcaacttc tccgacggag agctcgacct ggacgtcctg
     2341 ctcgagatga tccaagcctc cggggccccg gcgtgcgtct gcgagacccc atgggagggg
     2401 atcgcggagg acgtgaagca ggttaaagcg cgtatcgccg gctaaacggc gaggatctct
     2461 ttcagctcgg cgacgtgctt gccggcattg acgccgtaaa gagcctgttc gacgattccg
     2521 ttctcgtcga tcacaaaggt c
//
LOCUS       DATMED010000127         3710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5088973, whole genome shotgun sequence.
ACCESSION   DATMED010000127 DATMED010000000
VERSION     DATMED010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5088973"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VNP73_03190"
     CDS             complement(<1..812)
                     /locus_tag="VNP73_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWL64956.1"
                     /translation="MTVDHDIHDEGLADEGRLKVEWADRQMPVLQRIRERFEKEKPLD
                     GVRVAACLHVTTETANLMRTLQAGGADVALCASNPLSTQDETAAALVKAYDIPTFAIH
                     GVGDTEYYGHIKAVLDRKPMITMDDGCDLVSTLHRERQDQLGEIIGGTEETTTGVIRL
                     RSMEAAGVLGYPVISVNDADTKHLFDNRYGTGQSAIDGIIRATNVLLAGRTLVVCGYG
                     MCGRGVASRAKGMGADVVVVEIDPTRALEAAMDGFRVMQSRDAATVGDIFLTV"
     gene            857..1348
                     /locus_tag="VNP73_03195"
     CDS             857..1348
                     /locus_tag="VNP73_03195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWL64957.1"
                     /translation="MAIDLDIYREILTGFPSGVAVVTASGEDDKPLGLTVSAFCAVSL
                     QPPLVLVCIDKASNTLSAIQGSGGFTVNLLAAGREEIALAFAGKADDKFAGVQWRRPS
                     IPQSGPILDRDSVGYAECVTEKELEAGDHWIFVGRVENGASDPEASPLVYGRRQFAAW
                     SDL"
     gene            1404..2183
                     /locus_tag="VNP73_03200"
     CDS             1404..2183
                     /locus_tag="VNP73_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWL64958.1"
                     /translation="MTTNDLFSIEGKTACVTGGSRGIGLMIARGFVEAGAKVYISSRK
                     KEQCDETAAELSKIGECVAIPADLSTLDGIKSLAGGIQDKESELHILVNNAGAAWGVP
                     FEEYPEEGWDKVMDTNVKGIFFVTQQLLPLLRKAGTAEDPARVINISSVEAFRVPPWE
                     NYAYPASKAAVVQLTCHLAKNLASDNITVNAIAPGLFPSKMTNFLFQDEAATEQFEQH
                     IPLQRHGKPSDMAGPAIFLASKAANYMTGSTIVVDGGSLLA"
     gene            complement(2184..>3710)
                     /locus_tag="VNP73_03205"
     CDS             complement(2184..>3710)
                     /locus_tag="VNP73_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64959.1"
                     /translation="NGAGINNNRVAYQDGWVFPPHPTLYRNGYSVLTQPEGAAVTKYL
                     ERVREKELRGRRFAVAADMHGPVPVGAVLLHDQGNTPEKLVGVHDLAERIKQAMDEVF
                     ASYFTEHGLAAHQLVAKYAGEARDEAMKIWTQIFGSQDEKAAYLTLQWAEYATIWEHI
                     DYTVSGSFGGWSNSNAGLGAESISFEIDCLSYEPWNPPLMQLFTDNIRAIAETSAVHA
                     ALKSKPVDRKNLKATVGYYDSGDRVTDADGNPSAPPKGFPGNPLVPQIKQTPYDVSNT
                     DYFRDLRQIVSSPLVEVNKPHVKDGLLHELDTFVISDTTFGKADVLERYVKEGGNLVL
                     TDGALRLVPELVDIDADFVQKNYSYVGYSDLIREHPMTDGLYDRARQMYDPIGLGYEL
                     LMERDQYWPCGNGCEKSPTKNSSPNWTIDRAEWERVGGETVGTADPPESRKGGAEGNE
                     TNKTNIGVLKLGKGKIIIFGALLPQPTERFPHWFGLNAYSVSIPGQQLLLKALAGKTN
                     "
BASE COUNT          669 a         1157 c         1142 g          742 t
ORIGIN      
        1 accgtcaaga agatgtcgcc cacggtcgca gcatcacggc tctgcatcac gcggaaccca
       61 tccatcgctg cttccaaggc ccgggttggg tctatctcga ccacgacgac gtcggctccc
      121 atgcccttcg cgcgagaggc aactccgcgg ccgcacatcc cgtacccgca cacgactagg
      181 gtcctaccgg cgagaagaac gttggtcgct cggatgatcc cgtcgatcgc gctctgcccg
      241 gtgccgtaac ggttgtcgaa aagatgcttc gtgtcggcgt cgttaacgct gatcaccggg
      301 tagccgagca cgccggccgc ttccatcgaa cgcaaccgga tgacccccgt cgtggtttcc
      361 tcggttccgc cgatgatctc gcccagctgg tcctggcgct cgcggtgaag cgtcgagacg
      421 agatcgcaac cgtcgtccat cgtgatcatg ggcttgcggt cgagaacggc cttgatgtgc
      481 ccgtagtact cggtgtcccc cacgccgtgg atcgcgaagg tcgggatgtc gtatgccttc
      541 acgagggcgg cggccgtctc gtcctgggta gacagggggt tggatgcgca cagggccaca
      601 tcggcccccc cggcctgcag ggttctcatc agattggccg tttcggtcgt tacatgcagg
      661 caggcggcga cccgaacgcc gtcgaggggc ttctccttct cgaaacgctc cctgatgcgt
      721 tgcaggaccg gcatctgccg gtcggcccac tcgaccttga gtcggccttc gtcggccaaa
      781 ccttcatcgt ggatgtcgtg atcgacggtc attggactcc cctttcgttt caggagaaat
      841 taccctacgg accaccatgg cgatcgacct cgacatctat cgcgagatcc tcacgggctt
      901 tccgtccggt gtcgcggtcg tcacggcctc gggtgaggac gacaagcccc tgggccttac
      961 ggtcagcgcc ttctgcgcgg tgtcgctgca acctccactc gtgctcgtct gcatcgacaa
     1021 ggcttccaac acgctctccg ccatccaggg gtcgggtgga ttcaccgtca atctcctcgc
     1081 cgcgggccgt gaggagatcg ccctcgcctt cgcgggaaag gcagacgaca aattcgcagg
     1141 ggtgcagtgg cgtaggccat cgatcccgca gagcggaccg atcctggatc gtgactccgt
     1201 cggctacgcg gaatgcgtca cggagaaaga actcgaggcc ggtgatcact ggatcttcgt
     1261 cggtcgggta gagaacggag cctccgatcc cgaggcaagc ccactggtct acggccggcg
     1321 tcagttcgct gcttggagcg atctctaatt acctttggcg cgccggccga gtccggctct
     1381 agaagattcg ctagagtcgg cggatgacaa ctaacgacct gttctccatc gaaggaaaga
     1441 cggcctgtgt aacgggcgga tcgcgcggga tcgggctgat gatcgctcgc gggttcgtcg
     1501 aagccggcgc caaggtctac atctcgtcgc gcaagaagga gcagtgcgac gagacagcag
     1561 ccgaactctc gaagatcggt gaatgcgtcg ccatcccggc cgacctctcc actctcgatg
     1621 ggatcaagag cctcgccgga gggatccagg acaaggaatc ggagctgcac atcctcgtca
     1681 acaacgcggg agcggcttgg ggcgtcccct tcgaggaata tccagaagag ggttgggaca
     1741 aggtgatgga cacaaacgtc aaggggatct tcttcgtgac gcaacagttg ctcccgctgc
     1801 tgcggaaagc cgggacggca gaggatccgg cccgcgtgat caacatctcg tcggtcgaag
     1861 ccttcagggt cccgccgtgg gagaactacg cgtaccctgc aagtaaagcg gccgtcgtgc
     1921 aactcacatg ccatcttgcg aagaacctcg catccgacaa catcaccgtg aatgcgatcg
     1981 cgccgggcct gttccccagc aagatgacga acttcctctt ccaggacgaa gccgcgacag
     2041 aacagttcga acaacacatc ccactgcaac ggcacgggaa gcccagcgat atggcggggc
     2101 cggcgatctt cctcgcgtcg aaagctgcca actacatgac cggctcgacc atcgtggtcg
     2161 acggagggtc gcttcttgcg tagttagttc gtcttaccgg cgagcgcttt caatagaagt
     2221 tgctgacccg gtatcgatac tgagtaagcg ttgaggccga accagtgcgg gaacctctcg
     2281 gtcggctgcg gcagtagagc tccgaagatg atgatcttgc ccttgccgag cttcaggact
     2341 ccgatgttcg tcttgttggt ctcgttcccc tcggcgccgc ccttgcggct ctcgggagga
     2401 tctgcggttc cgaccgtctc tcccccgacc cgttcccatt cagcccggtc gatggtccag
     2461 ttgggagacg agttcttggt gggcgacttc tcgcagccat tgccgcaggg ccagtactga
     2521 tctcgctcca tcaacagctc gtagccgagt ccgatcgggt cgtacatctg gcgagctctg
     2581 tcgtagagac cgtcggtcat cggatgctcc ctgatcaggt ccgaataacc gacgtagctg
     2641 tagttcttct ggacgaagtc cgcgtctatg tcgacgagct cggggaccaa ccgcagggcg
     2701 ccgtcggtga gcacgagatt cccgccctct ttgacgtagc gctccagcac atccgccttc
     2761 ccgaaggtcg tgtcggagat cacgaacgta tcgagttcgt ggagtaggcc gtcctttacg
     2821 tggggcttgt tcacctcgac gagcggcgat gaaacgatct gacgcaggtc cctgaagtag
     2881 tcggtgttcg agacgtcata tggcgtctgc ttgatctgag gcacgagtgg attgccgggg
     2941 aagcctttcg gcggcgccga cgggttgccg tcggcgtcgg tcacgcgatc gcccgagtcg
     3001 taatagccga cggttgcctt gaggttcttc cgatccaccg gcttcgactt gagcgcggcg
     3061 tggactgccg acgtttcggc gatcgcccgg atgttgtccg tgaagagctg catcaacggc
     3121 gggttccagg gctcgtagga caagcagtcg atctcgaacg agatggattc ggcccccaac
     3181 cctgcgttgc tgttggacca acctccaaag gagccggaca ccgtgtagtc gatgtgctcc
     3241 caaatcgtgg cgtattcggc ccactggagc gtgaggtagg cagccttctc gtcctgcgac
     3301 ccgaagatct gggtccatat cttcatcgct tcgtcgcgcg cttctcccgc gtatttggcc
     3361 acgagctgat gagccgcgag cccgtgctcg gtgaagtaag acgcgaacac ctcgtccatc
     3421 gcctgcttga tgcgttcggc gagatcgtgg acgcccacga gtttctcggg ggtgttccct
     3481 tggtcgtgca ggagaaccgc accgacggga acgggtccgt gcatgtccgc ggcgaccgcg
     3541 aaccgacgtc cccgcagttc cttctcgcgc acacgttcca ggtatttggt gacagcggcc
     3601 ccctcgggct gagtcagaac cgaatacccg ttccgataga gcgtcgggtg gggcgggaaa
     3661 acccatccat cctggtatgc gacgcggttg ttattgatcc cggcgccgtt
//
LOCUS       DATMED010000128         5127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3169906, whole genome shotgun sequence.
ACCESSION   DATMED010000128 DATMED010000000
VERSION     DATMED010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3169906"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /gene="aroE"
                     /locus_tag="VNP73_03210"
     CDS             complement(<1..445)
                     /gene="aroE"
                     /locus_tag="VNP73_03210"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWL64960.1"
                     /translation="MRPRHYTSFAGVIGWPLQHTLSPTIQNAALRSVGIDWVYLALPV
                     PPENLADAIQGLRVLGAMGINVTMPHKESVIEHLDEVSGDAREVGAVNTIQRLGDRLI
                     GHNTDVDGFRDLMLGDAGIDVAGKRALVLGAGGAARAVVKALDDMG"
     gene            complement(442..1500)
                     /gene="mltG"
                     /locus_tag="VNP73_03215"
     CDS             complement(442..1500)
                     /gene="mltG"
                     /locus_tag="VNP73_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWL64961.1"
                     /translation="MSTPQADSGVRLTRRGRVITLLIALALVLAVASLAGLLYLRGIG
                     VLGSSDPGERVEFAIPQGASAEEIGKILEQEGVVTSAFGFRLAVFLEGGGEAIQAGRY
                     ELRTNLTARDALGALAKGPVIEFVSVTFPEGSWLTDFATTVDRRTHLSAEKFLRLAQG
                     RVRSGLQPEEVNTLEGLLFPSTYQVVESDTERSVLKRLIGQMEKETAAVDFESKAEAL
                     GITPYEALVVASMIEGEASAAGDRDRIARVIYNRIEMDMKLEIDATVLYALGEHKEEL
                     TRSDLDVDSPYNTRRYPGIPPTPIGAPGAASLAAAVNPAEGDWLFYVLADCDGNHFFT
                     DDYNAFLRQKAEYQALDC"
     gene            complement(1497..1919)
                     /gene="ruvX"
                     /locus_tag="VNP73_03220"
     CDS             complement(1497..1919)
                     /gene="ruvX"
                     /locus_tag="VNP73_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWL64962.1"
                     /translation="MTDKERPTILAIDPGGRRYGVAIADLETRFARPLEVIDATLQDP
                     IARIADLIRDCKVVRVVVGRPVALSGSNGPAVAGNREFIEKLGDLGIEVVEHDERLTT
                     VIAEQGLRAGGSKRATRERLRDAVAAQVLLQHFLDSQR"
     gene            complement(1916..4558)
                     /gene="alaS"
                     /locus_tag="VNP73_03225"
     CDS             complement(1916..4558)
                     /gene="alaS"
                     /locus_tag="VNP73_03225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWL64963.1"
                     /translation="MDSGTVREQFFKFFEARDHDRVPSSSLVPNDPTLLLTNAGMVPF
                     KPYFLGEQTPPFKRAISSQKCMRAGGKHNDIEEVGRTPRHLTFFEMLGNFSFGDYYKE
                     KACPWAWELVTETFGLDEERLWVTIFQTDDEAYSIWADEVRVDPKRIVRRGKEDNFWS
                     MGIAGPCGPCAEIMVDLGDDFGPPSDLGPAGNEDRYLEIWNLVFMENDCNAQIEPVAE
                     LPKRNIDTGAGLERMAMVLQNKSSVFETDVLRHMIDRAEELTKKTYGRDGSDDVGLRI
                     LADHGRSVAFMLADGVLPSNEERGYVLRLIMRRAIRRARLLGTTEPILGGLIERAVEL
                     MGDAYPELGAKKPLSLEVAAREEERFDQTLQQGLALLEKEVAGLKSQGSKELAGDVAF
                     RLHDTWGFPIDITTEIAAEHGLGLDRSGFDHLMQEQRERARAARRYDAAGVAADAAAS
                     VVDGSGPTEFLGYEHLRSDARVVALLRGAESVSVVSEGEEVDIVLDRTPFYAESGGQV
                     GDQGDLVLSSGRARVFDTQRVVPGLLAHRARVTAGELAVGDDVDAIVDMEKRVGAERA
                     HTATHIVHWILRDRLGDHVRQAGSLVEPGRLRFDFTHFDAIAPGQLSEISSELQEKVM
                     FDDDVRAYETSFEFAKSIGALAFFGEKYGDFVRVVEVGEYSKELCGGTHVPHTSQIGV
                     VVVTGEGSVAANTRRVEALVGREGLTFLERRARAVEEMASMLKAGPDDVTEKLERLLE
                     THKQMERKLADLDKKESESEADSLAASAVDVDGTRLVVARRDLDVDSLRSLAQSLRSK
                     LGSGVIVLGAAGEGRANLVGAVTKDLTAKGISARELLAPGAELLGGGAGGKPELAVSG
                     GPSADRLEDAMEAVAQAARAALAR"
     gene            complement(4582..4767)
                     /locus_tag="VNP73_03230"
     CDS             complement(4582..4767)
                     /locus_tag="VNP73_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64964.1"
                     /translation="MMIKTVFWVAVGAAGALQSEKLASKLRARFSPRAVTDGLLDKVN
                     SRLESQQAKKDVPAPGL"
     gene            complement(4777..>5127)
                     /locus_tag="VNP73_03235"
     CDS             complement(4777..>5127)
                     /locus_tag="VNP73_03235"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWL64965.1"
                     /translation="FVGLPEAKLNLAQAAVYLALAPKSNASAVALWRAEEDVKGSGPL
                     PVPTHLRDSHSPASRSIGAGEGYRYAHAHGGWVEQHHLPEELRERRYFQAIAGREAKL
                     AADLDAKKREEEPG"
BASE COUNT          910 a         1778 c         1483 g          956 t
ORIGIN      
        1 tacccatgtc atcgagagcc ttcacaaccg cacgcgccgc acctcctgca ccgagcacca
       61 gcgcgcgctt cccggccacg tcgatgccgg catcgcccaa catgaggtcc cggaaaccgt
      121 cgacgtcggt gttgtgacct atcaggcggt caccgagtcg ttggatcgtg tttactgcac
      181 cgacttcgcg ggcgtctcca gatacctcgt cgagatgctc gatcaccgat tccttgtgag
      241 gcatcgtcac gttgatcccc atcgcgccga gaacccgcag gccctggatc gcatcggcca
      301 gattctcggg aggaacaggc aacgctaggt aaacccagtc gatgcccacg cttctaaggg
      361 ccgcattttg gatcgtcgga gaaagggtgt gctgaagagg ccacccgatg actccggcga
      421 acgacgtgta gtggcgaggt ctcaacagtc cagagcctgg tactcagcct tctggcgcaa
      481 gaacgcgttg tagtcgtcgg tgaagaaatg gttgccgtcg caatcggcga gcacgtagaa
      541 gagccagtct ccttctgccg ggttcactgc cgcagcgaga gaggcggcac ccggcgcccc
      601 tatcggagtg ggaggaatcc ccgggtaacg acgggtgttg tagggcgaat ccacgtcgag
      661 atcggagcgg gtcaactcct ccttgtgttc ccccaaggca tacagaacgg tcgcgtcgat
      721 ctccagtttc atgtccatct ctatccggtt gtagatgacg cgcgcgatcc tgtcgcgatc
      781 gccggccgcg ctggcttcgc cctcgatcat cgaagcaacc accagtgcct cgtagggcgt
      841 gattcccaga gcttcggcct tcgactcgaa gtcgaccgcc gccgtttcct tctccatctg
      901 gccgatcaat cgcttcagaa ccgaacgctc cgtatccgac tcgacgacct gataggtcga
      961 agggaacagg agcccttcca gggtgttcac ctcttccggt tgcaaccccg agcggacgcg
     1021 cccctgcgcg aggcggagga acttctctgc cgaaaggtgg gttcttctgt cgacggtcgt
     1081 cgcgaagtcg gtcagccacg atccttcggg gaacgtgacc gagacgaatt cgatcacggg
     1141 acctttcgcc agagccccca gcgcgtcgcg cgccgtgagg ttcgtgcgca actcataccg
     1201 gcccgcctgg atggcctcgc cgcctccctc caggaagacg gcgaggcgga aaccgaacgc
     1261 ggacgtgacc acaccttcct gctcgaggat cttgccgatc tcttccgcag acgcaccttg
     1321 cgggatcgcg aactcgaccc gctcccccgg atcgctcgag cccagcacgc cgatgccgcg
     1381 gaggtaaagg agccctgcca gcgacgccac cgcgaggaca agggccagag cgatcagcag
     1441 agtgatgaca cggccccgcc gcgtcagcct tacgcccgag tcggcctgcg gggtgctcat
     1501 cgttgcgagt ccagaaaatg ttgcaagagc acctgcgccg ccacggcgtc ccgcaacctc
     1561 tctcgggttg ctctcttgct gccgccggct cgcaaaccct gctcggcgat caccgtcgtg
     1621 agtcgttcat cgtgttcgac gacctcgatg cctagatcac caagcttctc gatgaactcg
     1681 cgattgccgg caaccgcggg gccgttcgac cccgatagtg ctaccggacg tccgaccaca
     1741 acgcgaacca ctttgcagtc gcggatcagg tcggcgatcc tggcgatcgg gtcctgcagt
     1801 gtcgcgtcga tcacctcgag cggccgggcg aaacgggtct cgagatcagc gatggcaacc
     1861 ccgtagcgcc gacccccggg atcgatcgcg aggatcgtgg gtcgttcctt gtctgtcacc
     1921 gagcgagagc ggcgcgcgct gcctgcgcaa cggcctccat agcatcctcg agtcgatcgg
     1981 cggacggccc tcccgaaacg gccagctcgg gcttgccgcc ggcacctccc cccaacagtt
     2041 cggcccccgg agcaagaagc tcgcgtgcag agatcccttt cgcggtcaga tcctttgtca
     2101 ccgcacccac cagattggcg cgaccttcgc cggcggcgcc gagcacgatg acccccgaac
     2161 ccagcttcga tctcagtgac tgcgccagcg acctcaacga gtcgacgtcc aggtcccggc
     2221 gcgccacaac gagcctcgtc ccatcgacat ccaccgccga cgcggcgagt gaatccgcct
     2281 cgctttcgga ttccttcttg tcaagatctg cgagcttgcg ttccatctgt ttgtgggtct
     2341 cgagcagccg ctccaacttc tcggtgacgt cgtcgggacc tgccttcaac atggacgcca
     2401 tctcttctac agcgcgggcg cgtctctcca agaaggtcaa tccctcacga cctacaagag
     2461 cctcgacccg gcgagtgttg gccgcgaccg agccttcgcc cgtgacgacc acgacgccga
     2521 tctgacttgt atgcggcacg tgcgtcccgc cgcacagctc cttggagtac tcccccacct
     2581 cgacgacccg aacgaagtcg ccgtatttct cgccgaagaa agcgagcgct ccgatcgact
     2641 tcgcaaactc gaaactcgtc tcgtacgccc taacgtcgtc gtcgaacatc accttttcct
     2701 gtaactcgct cgagatctcc gacagctggc cgggagcgat cgcatcgaag tgcgtgaagt
     2761 cgaatcgcaa cctgccgggt tcgaccagcg acccggcctg cctaacgtga tcacccagac
     2821 gatcccgcaa gatccaatga actatgtgag tggcggtgtg ggcgcgctcc gcgccgacac
     2881 gcttctccat atccacgatc gcgtcgacat cgtcgccgac ggccagctcc ccggcagtaa
     2941 cccttgcgcg gtgcgcgagc aaacccggta cgacccgctg cgtatcgaag acgcgcgctc
     3001 ggccactcga caacaccagg tcgccctgat ctcctacctg gcctccactc tccgcataga
     3061 agggtgttcg gtcgagcacg atgtcgacct cctccccttc cgaaacgacg ctcaccgact
     3121 cggctccgcg aagcagcgcg acgacgcgag catccgaacg gaggtgctcg tatccgagga
     3181 attcggtagg tcccgatccg tcgaccacag acgccgccgc gtccgctgcg acccccgccg
     3241 cgtcgtatcg gcgagcggcc cgtgctctct cccgctgctc ctgcatcagg tgatcgaacc
     3301 cactccggtc gagccccagt ccgtgctccg ccgcgatctc ggtagtgatg tcgatcggga
     3361 atccccacgt gtcgtgtagc cggaaggcga cgtcgccggc cagctccttg gatccctgcg
     3421 acttcagtcc ggcaacctcc ttctccaaga gcgcaaggcc ttgctggagc gtctggtcga
     3481 aacgctcctc ctctctggcc gcaacctcca gcgacagagg cttcttggcc cccagttcgg
     3541 ggtacgcatc gcccatcagc tcgacggctc gctctatcaa gcccccgagg atgggttccg
     3601 tggtccccag caaccgggcc cgccggatcg ccctgcgcat gatcaggcgc aagacgtagc
     3661 ccctttcctc gttcgaaggc aggaccccgt cggccaacat gaacgccacg ctgcgaccgt
     3721 gatccgcgag gatcctcaag ccgacgtcgt cggacccatc ccggccgtaa gtctttttcg
     3781 tcagttcctc ggcccgatcg atcatgtgac gcaacacgtc ggtctcgaac acgctgctct
     3841 tgttctgaag caccatcgcc atgcgctcga ggccggcgcc ggtgtcgatg ttcctcttag
     3901 gtagttcggc taccggctcg atctgcgcgt tgcagtcgtt ctccatgaac acgaggttcc
     3961 agatctcgag atagcggtct tcattacccg cggggcctag gtcgctgggg gggccgaagt
     4021 catcgccgag gtcgaccatg atctcggcgc acggcccgca cggacccgcg atccccatgg
     4081 accagaagtt gtcctctttc cccctacgaa cgatccgttt gggatcgact cggacttcgt
     4141 cggcccagat cgaatacgcc tcgtcgtcgg tctggaagat cgtgacccac agccgctctt
     4201 catccagccc gaaggtctcg gtgacgagct cccaggccca cgggcaggct ttctctttgt
     4261 agtagtcgcc gaacgagaag ttgcccaaca tctcgaagaa cgtcagatgt cgaggcgttc
     4321 gacccacctc ttctatgtcg ttgtgcttcc cgcccgcgcg catgcacttc tgggacgaga
     4381 tggcacgttt gaagggagga gtctgctcgc cgaggaaata gggcttgaac ggcaccattc
     4441 cggcgttcgt aagtaggagt gtggggtcgt tcgggacgag cgacgacgac ggcacccggt
     4501 cgtgatcccg cgcctcgaag aacttgaaga actgctcccg taccgtgccc gaatccatgc
     4561 cgacagggta cttggggctt ctcagagtcc gggggccggc acgtccttct tcgcttgctg
     4621 cgattcgagg cgcgagttga ccttgtccag cagcccgtcg gtcacggctc gaggcgagaa
     4681 gcgcgcgcgc agtttcgacg ccagcttctc gctctgcaaa gctccggcag caccaaccgc
     4741 gacccagaac accgtcttga tcatcatgga ggcaccctat cccggctcct cctctcgctt
     4801 ctttgcgtcc aggtccgcag ccaacttagc ttctcggccg gcgatcgcct ggaagtacct
     4861 gcgctcgcgt agctcctcgg gaaggtggtg ctgttccacc caaccgccgt gtgcgtgtgc
     4921 gtagcggtat ccctcgcccg ctccgatcga gcgcgacgcg ggtgagtgcg agtccctcag
     4981 atgggtgggc accggcaatg gcccggagcc tttcacgtcc tcttccgccc gccacagcgc
     5041 gaccgcgctc gcgttcgatt tgggggccag agcaagatag accgcggcct gcgcaagatt
     5101 cagtttggct tccggtagac caacgaa
//
LOCUS       DATMED010000129         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2408336, whole genome shotgun sequence.
ACCESSION   DATMED010000129 DATMED010000000
VERSION     DATMED010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408336"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VNP73_03240"
     CDS             <1..906
                     /locus_tag="VNP73_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWL64966.1"
                     /translation="RPGHDAYSGAPAGLIAIAVAAALWAVAAALARVLFDDGVDPLEL
                     VQARALLSAAGLALVPAAWRRPKEGGRLRHVIALGLCIALVNAAYYIAIQKLAVAVAI
                     VLQYLAPALVVVWTAVKLRRRPTTEVITAVVVAFVGVVLVSELPGADLGNLNFPGLVA
                     GFAAAVLFASYTLLSEKAGESYGVLGALFRGFVAASVCWIVFQSFRGWPSALFTAEHL
                     PFVLFIGIGGTLAPFLLYLWGVGRVRAERATIAATLEPVLVALIAWIWLGQALSLWQI
                     VGGVLIMGAVLSLQLVRHEVHAPEP"
     gene            complement(903..1949)
                     /locus_tag="VNP73_03245"
     CDS             complement(903..1949)
                     /locus_tag="VNP73_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWL64967.1"
                     /translation="MKHFDVAIVGAGVMGSATAHALARSGRKTVVFEQFGPHHERGSS
                     HGRSRIFRYSYPESRYVEMAMVSAELWREVEASVGLEGQLLVQIGGLDSGDEIEKNAA
                     ALAGCGAAFELLEGREAQVRWPSFRFPDDFPVLFQPDGGIVRASAAIDAFQAFALSHG
                     TQIHHESRVHEITPGSDSVTLHVGEEQVTARGVVVAAGAWVRKLLVPLGIDVPVRPTR
                     ETVAYFRVAGDPGPTLVEWGEPAIYAQRSPGHEGIKVGEHIAGPEADPDETGPVDASS
                     VERLTRWVLERYAGAEPDPLHAETCFYTNTDDQRFILERHDRIVIGSPCSGHAFKFAP
                     WIGRHLASLAEEAL"
BASE COUNT          334 a          709 c          652 g          409 t
ORIGIN      
        1 cgacccggac acgatgccta ttccggggcc ccagcgggcc tcatcgcgat cgccgttgcg
       61 gcggccctgt gggcggtcgc ggccgctctg gcccgggtcc tgttcgacga cggggtcgac
      121 ccgctcgagc tggtccaggc ccgggcgctg ctgtcggccg ccggcctggc tctcgttccg
      181 gcggcgtggc gccggccgaa agaagggggc aggctgcgac acgtcatcgc tctggggctc
      241 tgcatcgcct tggtcaacgc cgcttactac atcgcgatcc agaagctcgc cgtcgcggtc
      301 gcgatcgtcc tgcagtacct cgcccctgct ctcgtcgtcg tgtggacggc cgtgaaactg
      361 cgtcgccgac ccacgaccga ggtgatcacc gcagtagtgg tcgctttcgt cggtgtggtg
      421 ctcgtctccg agcttccggg agccgatctc ggcaacttga acttccccgg tctcgttgca
      481 ggcttcgccg ccgcggtctt attcgcaagc tacacgttgc taagcgaaaa ggcaggtgaa
      541 agctacggcg tcctcggtgc tcttttccgc ggtttcgttg ccgccagcgt ctgctggatc
      601 gtgttccagt cattccgtgg gtggccctcc gcactcttca ccgccgagca cttgccgttc
      661 gtgttgttca tcggcatcgg tgggactttg gcaccgttcc tgctttacct gtggggagta
      721 ggacgggtgc gagccgaacg agcaactatc gccgcgaccc tagagccggt cctcgtcgcg
      781 ttgatcgcgt ggatctggct cggtcaggca ctgtcgttgt ggcagatcgt cggaggtgtc
      841 ctgatcatgg gcgcggtcct gtcgcttcaa ctcgttcgac acgaggtgca cgcgcccgaa
      901 ccctagagtg cctcctcggc cagagatgca aggtgccttc cgatccacgg tgcgaacttg
      961 aatgcgtgcc cgctacatgg agagccgatc actatccggt cgtgacgttc gaggatgaag
     1021 cgctggtcgt cggtgttcgt gtagaagcac gtttcggcat gtagcgggtc tggttcggcc
     1081 cccgcgtaac gttcaagtac ccaacgcgta agacgttcga ccgacgacgc gtccactggg
     1141 ccggtctcgt cgggatcggc ttcagggccg gcgatgtgct cccctacctt tatgccttcg
     1201 tggcccgggc ttcgctgcgc gtagattgcg ggttcgcccc actcgacgag cgtcggaccg
     1261 ggatctcccg ccaccctgaa gtaggcgacc gtctcgcgcg tcggcctcac gggcacatcg
     1321 atccccagag gcaccagcaa cttccgcacc cacgcgccgg cggccacgac gaccccgcgc
     1381 gcggtgacct gttcctctcc gacgtgcaga gtgaccgaat cggagccggg ggtgatctca
     1441 tgcacacggg actcgtgatg gatctgggtg ccgtggctca acgcgaacgc ctgaaatgca
     1501 tcgatcgccg cgctcgctct cacgatccct ccatcgggtt gaaagagaac cgggaagtcg
     1561 tcggggaacc ggaacgacgg ccagcgcacc tgcgcctccc ggccttcgag caactcgaag
     1621 gccgcgccac atcccgccag cgcagcggcg ttcttctcga tctcgtcgcc tgaatcaagg
     1681 cccccgatct gaaccagcaa ctgcccctcg agacccaccg acgcttcgac ctcgcgccac
     1741 agctccgccg ataccatcgc catctcgacg tagcgagact cgggatacga gtaacggaag
     1801 atgcgagacc gtccgtgact cgagcctcgt tcgtggtggg ggccgaactg ctcgaacacg
     1861 acagtcttgc ggccgcttct cgccagtgca tgggccgtcg cagagcccat cacgccggct
     1921 cccacgatcg ccacatcgaa gtgcttcatg gccccagtat ttgcacgaat cgccgtatcg
     1981 gttaatggtt cccggtcatg actgagatcg cgttcctaga cgcaaccgag caagcccgcc
     2041 tggtgcggga caaggaagtc tctccggtag agctggtcca gacttacctc gaccggatcg
     2101 acaa
//
LOCUS       DATMED010000130         4755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1636475, whole genome shotgun sequence.
ACCESSION   DATMED010000130 DATMED010000000
VERSION     DATMED010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636475"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VNP73_03250"
     CDS             <1..241
                     /locus_tag="VNP73_03250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWL64968.1"
                     /translation="DDIFTELKAFFAVCGQEGVPPGGVHLELTHEAVTECLGGAEEVL
                     DANLSERYLALCDPRLNARQSLDLAFQLGEMLQQN"
     gene            complement(238..471)
                     /locus_tag="VNP73_03255"
     CDS             complement(238..471)
                     /locus_tag="VNP73_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(439..441),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HWL64969.1"
                     /translation="MEISIEYCAQUGYAPRAVGLTERILEEFEHKIEGVTLVPSRGGV
                     FEVVCDGELVFSKKKTKRHAEHDEVLAAIRKIA"
     gene            538..1176
                     /locus_tag="VNP73_03260"
     CDS             538..1176
                     /locus_tag="VNP73_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWL64970.1"
                     /translation="MKLDPISLIVIISIPLLVVFLIATYNRLVQLRNRTEAAWSQIDV
                     QLKRRYDLIPNLVETVKGYAAHERQTLEAVIQARQQAVNAQGIENQAQAENFLTQALR
                     QLLAVAEAYPDLKANQNFLALQEELAATEGRIAYARQFYNESVLAYDNALEKFPSNIV
                     GGAFNFKPSEYFEAETESRENVAVDFGQGAGAPSATPPTPDAPPPATPPAAS"
     gene            1195..2148
                     /locus_tag="VNP73_03265"
     CDS             1195..2148
                     /locus_tag="VNP73_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWL64971.1"
                     /translation="MLYDRVASNVRKTWLLIVVFSLVVLFVGWFFGYYLGIGYWGLVG
                     ATILAVVMTWGSYFSSDKIALSMSRAKPADEVQHRQLHNIVEGLALAAGLPKPRVYVI
                     DDTAPNAFATGRNPEHAAVAVTRGLIARMNREELEGVIAHELSHIKNRDTLVMTLAVT
                     LVGILVLLADWFLRSLWWGGGNRDRGGGGLGAPIAVLGLLMMVFAPIIAQLLQFAISR
                     RREFLADADGVLLTRYPQGLIGALEKLKEDPTVIRTASKATAHLWIESPIARQLGEGE
                     GKGKRGLWLNRMFDTHPPLDERIEALRGELEGAPQPPAASA"
     gene            complement(2124..3401)
                     /locus_tag="VNP73_03270"
     CDS             complement(2124..3401)
                     /locus_tag="VNP73_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL64972.1"
                     /translation="MRRPRFLPDLPRRAWVLLAGDCISFFGNGLILPFTIIYLTRARG
                     IPIEIAGFALAVRSVVGLFVGPVAGTLVDRIGARRVLIFAQVTTAVATVSFIFVHEPW
                     QAFLASALNGIGWSSFWPSMQSMLSTVVDPSQRSPMFAVHYASLNLGIGIGGIIGGVV
                     ANISDPASFERLFAIDALTFLPVIAILLLAPSLAGAGRATDIDQAPATEADEVGPSGY
                     LQVLKDRVFVRVVILMVVLTTVGYSQLESSFPAFATGEGGISTAMLGLAFAGNTFFIV
                     IAQLLVLRRIEGVRRTRAITVLCGAWALCWAATIAAGVVDGTAAAVGFISAMVLFAFG
                     ETLLSPTIPAIVNDLAPERLRGRYNALYSFAWNGGTFFGPALAGLFLGAELALPFFAG
                     LVVACGLAGAYSLRLERHLPPGTNQIQAEAAGG"
     gene            complement(3398..4048)
                     /locus_tag="VNP73_03275"
     CDS             complement(3398..4048)
                     /locus_tag="VNP73_03275"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWL64973.1"
                     /translation="MEKLATPNGEVSLAVDEAGAPRAALVLGHGAGSGMESPFMAEFA
                     SGLAQRGVTTYRFNFLYTEQGRKAPDRQPILEATFAAVAEAVAEREPRPVFLGGKSLG
                     GRIASHVVASGGNAAGLAFLGYPLHPPGRPDRIRDAHLIEISVPMLFVEGTRDPFCPL
                     DTLEKVRGRLKARSEVAVIEDGDHSLKVRKSSGRDSGAAWAEAMDAVAAWIVRESG"
     gene            4121..>4755
                     /locus_tag="VNP73_03280"
     CDS             4121..>4755
                     /locus_tag="VNP73_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HWL64974.1"
                     /translation="MFEKILMTLLLLASLGAFALRARDLVGYLKLGSGEDRRPRSWAQ
                     KLKEQIVVVFGQRKLLQWTIPGLMHFFIFWGFVILFTTIVEAFGAVYQEGFHIPLIGK
                     WGPLGALQDFFVVAVLAGIVVALAIRKIQKPGRFRGSHLKEADYILYAITGIMFTILF
                     TRAAEISLGFFPYPTEWTPVSAGVANLFENFSLEAREAIDTVFLWWHSLIIL"
BASE COUNT          911 a         1516 c         1515 g          813 t
ORIGIN      
        1 ggacgacatc ttcacggaac tgaaggcgtt cttcgccgtg tgtgggcaag agggggtgcc
       61 cccgggcggg gtccacctcg agctcacaca cgaagcggtg accgagtgcc ttgggggggc
      121 cgaggaggtc ctcgatgcga acctctccga gcgctatctg gcgctctgcg acccgaggct
      181 gaacgcacgt cagtcgctgg acctggcttt ccagctgggg gagatgctcc agcagaacta
      241 ggcgatcttg cggatcgctg cgaggacctc gtcgtgctcg gcatggcgct tcgtcttctt
      301 cttggaaaag acgagctcac catcgcagac gacctcgaac accccgccac gtgaaggaac
      361 gagggtcacg ccctctatct tgtgttcgaa ctcttcgagt atgcgttccg tcagaccgac
      421 ggctctaggc gcgtaccctc attgtgcgca gtactcgatc gatatctcca tgtgatgaga
      481 gtctaggggt tgctcacggg cgacgactag gctaagagcc gcccgacacg aagggagatg
      541 aagttggacc ccatcagctt gatcgtgatc atcagtatcc ccctactggt cgtgttcctg
      601 atcgccactt acaaccggtt ggtgcagctg cgtaaccgca cggaggcggc gtggtcacag
      661 atcgacgtcc aactgaagcg tcgctacgac ctcatcccga acctcgtcga gacggtcaag
      721 ggttacgcgg cacacgagcg ccagacgctc gaagccgtga tccaggcgcg ccagcaagca
      781 gtcaacgcgc aggggatcga gaaccaggct caggccgaga acttcctcac gcaggcattg
      841 cgacagctgc tcgccgtcgc cgaggcttat cccgatctga aggcgaacca gaacttcctc
      901 gcgttgcaag aggagctagc cgcaaccgag ggccggatcg cctacgcgcg gcagttctac
      961 aacgagagcg tccttgcgta cgacaacgcg ctcgagaagt tcccgtcgaa catcgtcggc
     1021 ggtgcgttca acttcaagcc gagcgagtac ttcgaggccg agacggagtc gcgcgagaac
     1081 gtcgctgtcg atttcgggca gggagcaggg gccccgtccg caacgccccc gactcccgac
     1141 gcgccgccac cggcgactcc gccggcagcc tcctaacgcg cccggggatc gtcggtgttg
     1201 tacgaccgcg tcgcgtccaa cgtaagaaag acgtggttac tgatcgtcgt gttctcgctc
     1261 gtggtcctgt tcgtcggatg gttcttcggc tactacctcg ggatcggtta ctggggactc
     1321 gttggggcaa cgatcctggc cgtggtcatg acatggggct cgtacttctc ctccgacaag
     1381 atcgccttgt cgatgagccg ggcgaagccg gcggacgagg ttcagcatcg acagttgcac
     1441 aacatcgtcg agggcctggc tctcgcggcc gggctcccca agccccgcgt atacgtcatc
     1501 gacgacacgg cacctaacgc gttcgcgaca gggcgcaacc ccgagcacgc cgcagtggcc
     1561 gttacgcgcg ggttgatcgc acggatgaac cgagaagagc tcgagggcgt gatcgcccac
     1621 gagctgtcgc acatcaagaa tcgcgacacg ctcgtgatga ctctcgccgt cacgctcgtc
     1681 gggatcctcg tgctactggc cgactggttc ctgcgatctc tgtggtgggg aggagggaac
     1741 cgggatcgag ggggcggcgg cctcggcgcg cccatcgcgg tgctggggct cttgatgatg
     1801 gtgttcgcgc cgataatcgc tcagctgctc cagttcgcca tctcgcgccg ccgcgagttc
     1861 ctggccgacg ccgacggagt gctcctgacc cggtatccgc aagggctgat cggagccctc
     1921 gagaagctca aggaggatcc caccgtcatc cggacggcca gcaaggcaac ggcgcatctg
     1981 tggatcgagt ccccgatcgc ccggcaattg ggcgagggcg agggcaaggg caaacgaggg
     2041 ctgtggctga accggatgtt cgacacgcac ccacccctcg acgagcggat agaagccctt
     2101 cggggtgaac ttgagggtgc ccctcagccg ccggctgctt cggcttgaat ctggttcgtg
     2161 ccgggcggta ggtggcgttc caggcgcaac gagtacgcgc cggcgagacc gcacgcgacc
     2221 accaggcccg cgaagaacgg gagcgccagt tcggccccca agaagagacc ggcgagcgcg
     2281 gggccgaaga acgtgccgcc gttccacgcg aacgaataga gcgcgttgta gcggcctctg
     2341 agacgttcgg gggcaaggtc gttgacgatc gcggggatcg ttggagacaa cagcgtttct
     2401 ccgaacgcga acagcaccat cgcggagatg aatccgacgg ctgcagccgt gccatcgacc
     2461 acaccggcgg cgatcgtggc cgcccagcac aacgcccatg ccccacacag aaccgttatc
     2521 gcacgcgtcc gccgaacgcc ttcgatgcgt ctgaggacca gtaactgcgc gataacgatg
     2581 aagaaggtgt tcccggcgaa cgccagtccc agcatcgccg tgctaatgcc gccctccccg
     2641 gtagcgaacg cgggaaagga cgactcgagt tgcgagtagc cgacggtggt cagaacgacc
     2701 atgaggatca cgacgcgtac gaagacgcgg tccttcagca cctgtaggta acccgacggc
     2761 ccgacctcgt cggcctctgt tgccggtgct tgatcgatat ccgtcgcacg gccggccccc
     2821 gccagggacg gcgccagcag gaggatggcg atcacgggca ggaaagtcaa cgcgtcgatc
     2881 gcgaagagac gctcgaacga ggccggatcg gagatgttcg cgacgacacc accgatgatc
     2941 ccgccgatac cgatgccgag attgagcgac gcgtaatgaa ccgcgaacat cggcgaccgt
     3001 tgtgagggat ccaccaccgt ggacagcatc gactgcatgg atggccagaa cgacgaccac
     3061 ccgatgccgt tgagggcgct cgcgaggaac gcttgccacg gctcgtgcac gaagatgaac
     3121 gacaccgtag caacggcagt cgtcacctga gcgaagatga gcacgcgccg cgcgccgatg
     3181 cgatcgacga gggttccggc caccggtccg acgaacagcc cgacgacgga gcggaccgcc
     3241 agcgcgaagc cggcgatctc gatcgggatg cctcttgcac gcgtgagata gatgatcgtg
     3301 aacggcaaga tgagcccgtt cccgaagaac gagatgcaat cgccggccag gagcacccat
     3361 gcacgccggg gaagatccgg aaggaacctc ggtcgcctca cccgctctcg cgaacgatcc
     3421 aggcggcgac cgcgtccatc gcttcggccc acgcggcccc tgagtcccga cccgaggact
     3481 tgcggacctt taaagagtga tccccgtcct cgatcactgc gacttcggat cgggctttga
     3541 gccggccgcg caccttctcc aacgtgtcga gaggacagaa cggatcgcgc gtgccctcca
     3601 cgaacagcat gggaaccgag atctcgatca gatgagcgtc ccggatgcga tcggggcgac
     3661 cgggaggatg cagcggatac cccaggaatg cgaggccggc ggcattaccc cccgatgcga
     3721 caacgtgcga ggcgatccgg cccccgagag acttgccacc gaggaagacc ggacgcggct
     3781 ctctctcggc gaccgcctcc gcaacggccg caaaggtggc ctcgaggatc ggctgccgat
     3841 cgggagcctt gcgcccttgt tcggtgtaca ggaagttgaa gcgataggtg gtgacgccgc
     3901 gttgagctaa tccgcttgcg aactcggcca tgaacgggct ctccatgccc gaaccggctc
     3961 cgtggcccag taccagcgcg gcgcgcgggg cccctgcctc gtctacggcg agcgagacct
     4021 ctccgttcgg tgtagccagc ttttccacga ctcggaatcc taggaccggc gtggctagcg
     4081 cccctatact cctgccgcgt ctctgccgta ggaggtcgaa ttgttcgaga agatcttgat
     4141 gacgcttcta ctccttgctt cgcttggcgc gttcgcgctg cgagcccggg atctcgttgg
     4201 ctacctgaag cttggaagcg gtgaggacag aagaccgcgc agctgggcac agaagctcaa
     4261 agagcagatc gtcgtcgtct tcggccagcg aaagctcctg cagtggacga tccccggcct
     4321 catgcacttc ttcatcttct ggggcttcgt gatcctgttc acaacgatcg tcgaggcttt
     4381 cggggccgtt taccaagagg gcttccacat cccgttgatc ggcaagtggg ggccgctggg
     4441 agctctacag gacttcttcg tcgttgcggt gttggccggc atcgtggtcg cacttgccat
     4501 ccgcaagatc cagaagccgg gccgattccg tggttcgcac ctcaaagagg cggactacat
     4561 cctttacgcg atcaccggga tcatgttcac gatcctgttc accagggcgg ctgagatctc
     4621 gctcggcttc ttcccctatc cgacggagtg gacccccgtc tccgcgggag ttgcaaatct
     4681 gttcgagaac ttttcgctcg aggcccgcga agcgatcgac acggtcttct tgtggtggca
     4741 ctcgctgatc atcct
//
LOCUS       DATMED010000131         3622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3173223, whole genome shotgun sequence.
ACCESSION   DATMED010000131 DATMED010000000
VERSION     DATMED010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173223"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNP73_03285"
     CDS             <1..225
                     /locus_tag="VNP73_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWL64975.1"
                     /translation="GLVRRIEEQPAHHYLPAVSREQYARSTVKSVVDWLIGHFPDPAV
                     AYFLDRVEKEDERVIDRLKEAIDERRNTTD"
     gene            222..1625
                     /locus_tag="VNP73_03290"
     CDS             222..1625
                     /locus_tag="VNP73_03290"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HWL64976.1"
                     /translation="MTAPGPTVLAIETDSAWVVILVVSLATLALTWLLRSMIRAPGGL
                     ASGILLALPLALPLLAAVVFTHAVLPEISVWQPIGRALRDAPTELLHLLLVADRGSRT
                     VVPYALSGSAGQWMLLIGVAASSLMLLRRAFGAAMVRRLIARSVPLEATEHRALGPRL
                     HDLAVKSTLNGRVEILVLPDGVPGAFALGTRTRRILLGRELLTSLEEDELAAIAAHEF
                     AHLEANDVTLVFVAGLLRDLVAWNPVGHLAYRRLMADRELEADGRAADLTGNPLAVAS
                     GLLKVCKMMRAARYRHRVSLAFLSEGGGVKRRVASLIAAADGRISLAPSGLMPYAVAA
                     ALVAVLGLQVGARVAQDSPGAVMIMWGTPDSSDRTWSPDESRALRGGQSQLPAALPPQ
                     VEQVRPRVYGGPAGAGAVRLRDVPEWFAAMDRWTEKQRAEFIRMRWKSRQNWEATPLF
                     AHSSGPIDVYRLVPHPF"
     gene            1718..1945
                     /gene="secG"
                     /locus_tag="VNP73_03295"
     CDS             1718..1945
                     /gene="secG"
                     /locus_tag="VNP73_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018023986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWL64977.1"
                     /translation="MTAALVVIHVLASSTLILFVLLHAGRGGGVSEMFGGGMQTQAMG
                     STVMEKNLDRITVITAIIFTGTTILLAFRLQ"
     gene            1997..3610
                     /locus_tag="VNP73_03300"
     CDS             1997..3610
                     /locus_tag="VNP73_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL64978.1"
                     /translation="MAACLGAAACTDSDTVSGPRATAVATPEVTAVPSGSPTEQLPVF
                     DEEDAVLNVAIPEPATLDPMRLRDPGSVLVARQLYEGLVAWDPARERLVPAAAASWAA
                     SDGGRTFTFRLREGMTFHDGSPVTSRSFRFAFERIALKSSASDLAYALELIDGFTAVN
                     QAGSTRLAGISTPDPLTLVIRLSEPFYDFPAVLTHPGLVPLPSSAVADVDEFLAAPTG
                     NGPFRMAQTWSPGQPVILEAFPGFIQTPRLDGIRFLPYPDAAASWLPFVEGELHVAEV
                     PAGQIEEAREAFGGEGFQPFLAGYYYGFNITSPALKNGRLRTAINQAIDRAAIARTIY
                     NGTMEIARGIVPRGMPGFQEDICVNLCRYAPDSARRQVDKVRMKDRKVRLEYTQGEPH
                     GQVARFIKRDLETVGFEVDVKAYSFGKYLKRLRRKDQELYRFGWIAEYPSPDVFLSAL
                     FASDSPDNHSGFSSSKVDRLLDQAHAAASEGRREQLYIQAEKAIMRTLPVVPIGSFVT
                     HWAARPQVTGIRFDVMGGFDAAGVGLQED"
BASE COUNT          582 a         1189 c         1171 g          680 t
ORIGIN      
        1 ggcctcgtcc gccgtatcga ggagcaaccc gctcatcact acctcccggc cgtgtctcgt
       61 gagcaatacg cccgatcgac ggtcaagtcg gtggtggatt ggttgatcgg acattttccc
      121 gatccggccg tcgcctactt cttggaccgg gtcgagaagg aggacgaacg cgtgatcgat
      181 cgtctgaaag aggcgatcga cgagcggagg aacacaacag attgaccgcg ccggggccaa
      241 ccgtccttgc gatcgagacg gactcggcat gggtcgtgat ccttgtcgta tcactcgcca
      301 cacttgcttt gacttggttg ttgcgctcga tgatccgcgc gcccggtggt cttgcatcgg
      361 ggatccttct ggcgttacct ctcgccctgc cattgttggc ggcagtggtt ttcacccacg
      421 ccgtcctgcc tgagatctcg gtctggcagc cgataggacg cgcgcttcga gacgcgccga
      481 cggagttgtt gcacctgctt ctggttgccg atcgaggatc caggacggtc gttccctacg
      541 ctctgtccgg gtcggcgggt caatggatgc tgctgatcgg cgtcgccgcg agttccctga
      601 tgttgctgag gcgagctttc ggcgcggcga tggtccgccg actgatcgcg cgctccgtcc
      661 ccttggaggc caccgaacac cgggccctcg gcccgcgcct ccacgacctg gcggtcaaga
      721 gcacgcttaa cggccgggtc gagatactcg ttctgcccga cggcgttccg ggagcattcg
      781 ccctgggaac ccgcacccga cggatccttc tgggtcgcga gctcctcaca tcgctggagg
      841 aggacgaact ggcggcgata gccgcccacg agttcgccca tctggaagcg aacgacgtga
      901 ctctcgtttt cgtggccggc ttgcttcgtg acctggtcgc atggaatccg gtgggccatc
      961 tggcataccg gcggctcatg gccgatcgcg aactggaagc cgacggtcgt gcggccgacc
     1021 tgacggggaa ccctctggcc gtcgcgtcgg gcctgctcaa ggtgtgcaag atgatgcgcg
     1081 cggcgcgcta tcgccatcgc gtttcgctcg ccttcctcag cgagggtggg ggagtgaagc
     1141 ggcgcgtcgc gagcctgata gcggccgccg acgggcgcat ctcgttggcc ccctccgggt
     1201 tgatgccgta cgcagttgcc gccgctctcg tcgccgttct cggtctgcag gtcggcgcca
     1261 gggtcgcgca ggactcgccc ggggccgtca tgatcatgtg ggggaccccc gactcctccg
     1321 atcgcacgtg gtctcccgac gagtcacgtg ccctccgcgg gggccagagc cagctcccgg
     1381 cagccctccc gccgcaggtt gagcaggtgc gcccccgggt ctatggcggg ccggccgggg
     1441 caggcgcggt ccgcttacga gacgttcccg aatggttcgc cgctatggat cgatggacgg
     1501 agaagcagcg ggccgagttc atccggatga ggtggaagtc acgccagaac tgggaagcca
     1561 cgcccctttt cgctcattct tcggggccga tcgacgtcta tcgcctggtc ccgcacccgt
     1621 tctaaatctg gttctgcgcc acggccgagg ccgtggacgc gaccggctac cctggagata
     1681 cacaacaaac caaccagacg aaggaagtgg tgcaaccgtg acggccgccc tggtagtgat
     1741 ccacgttctc gcctcgagca ctctgatcct tttcgtcctg ctccatgccg gacgtggtgg
     1801 aggggtctcc gagatgttcg gcggaggcat gcagacacag gcgatgggat cgacggtgat
     1861 ggaaaagaac ctcgacagga tcaccgtgat caccgcgatc atcttcacgg ggacgacgat
     1921 ccttctagct ttccggcttc agtaggggtc cgcgaccttc ttgagggtca gaacgccacc
     1981 actcctagtc tgcatattgg ccgcctgtct cggcgccgcg gcctgtaccg attcggacac
     2041 ggtttcgggt ccgcgcgcca cggccgtcgc aacccccgag gtgacggccg ttccatccgg
     2101 cagtcccacc gagcaactcc ccgtcttcga cgaggaagac gcggtattga acgtcgcgat
     2161 ccccgagccg gccaccctcg atccgatgcg cctgagggac ccgggctccg ttctggttgc
     2221 gcgccagctt tacgagggcc tcgtggcctg ggatccggcc cgggagcgcc tggttcccgc
     2281 ggctgctgcc tcgtgggccg cgagcgatgg gggccggacc ttcacgttca gactgcggga
     2341 ggggatgacc ttccacgatg gctcgccggt aacgtcgcgg agcttccgat tcgccttcga
     2401 acggatcgcc ctgaaatcga gtgcgtcgga cctcgcctat gcactcgagc tcatcgacgg
     2461 attcacggcc gttaaccagg caggctctac gcggctcgcg gggatctcga cacccgaccc
     2521 gctcaccttg gtgatccggt tgagcgaacc gttctacgac ttccccgcgg tcctgactca
     2581 ccccggtctc gtcccattgc cgtcgagcgc ggtggccgac gtcgacgagt tcctcgcggc
     2641 ccccacaggg aacggcccct tccgcatggc gcaaacctgg tctcccgggc aaccggtgat
     2701 cctcgaagcg ttcccgggtt tcatccagac gcctcgtctg gatggcatcc gcttccttcc
     2761 ctatccggat gcagccgcgt cgtggctacc gttcgtagaa ggcgagttgc atgtagccga
     2821 agtcccggcc ggccagatcg aggaagcgcg tgaggcgttc gggggggagg gcttccagcc
     2881 tttcctcgcc ggctactact acggcttcaa catcacatca cccgcgctga agaacgggcg
     2941 gttgcgaacg gcgatcaatc aggccatcga tcgggccgcg atagctcgga cgatctacaa
     3001 cggaaccatg gagatcgcgc gcggcatcgt cccgcggggg atgccggggt tccaggaaga
     3061 catctgcgtg aacctatgcc gctacgcacc cgactcggcc cggcgccagg tcgacaaggt
     3121 caggatgaag gaccgcaagg tgcgtctcga gtacacgcag ggtgagccgc acgggcaggt
     3181 agcgcgtttc atcaagaggg atctcgagac cgtaggtttc gaggtggatg tgaaggccta
     3241 ctcgttcggc aaatacctca agcggctgcg caggaaggac caagagctgt atcgattcgg
     3301 gtggatcgcc gagtatccct ctcccgatgt gttcctgtcg gcgttgttcg cgtccgattc
     3361 gccggacaac cactccgggt tctcctccag caaggtcgat cggctgctcg accaagcaca
     3421 cgcggccgcc tcggaaggtc ggcgcgagca gctctacatc caggccgaga aggccatcat
     3481 gcgaacgctt cccgtggtgc ccatcggatc gttcgtgacc cattgggcgg cccgacctca
     3541 ggtcaccggg atccgcttcg acgtgatggg aggcttcgac gccgccggtg tgggactcca
     3601 ggaggactga cgttcgccgc gc
//
LOCUS       DATMED010000132         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1639779, whole genome shotgun sequence.
ACCESSION   DATMED010000132 DATMED010000000
VERSION     DATMED010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639779"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="VNP73_03305"
     CDS             <1..1876
                     /locus_tag="VNP73_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWL64979.1"
                     /translation="SGEIQEGASTITQQYVKQMIIAPGEIAEKSYERKIQEAALSRQI
                     EKELSKEEILERYLNTIYLGQGAYGFQAAAKVYFDVGVTDLTVSQGALIAGIIQRPGA
                     FDPFNHPRAGKRRRNLVLSRMQNLDYISEERAAKLSKKPLGLQKVNEKERDQFEAAYF
                     VDYVQKMLTYHPRFEMLGKTAAQRTKALFQGGLRITTTVDLDMQRAADNAVNSILYDA
                     SDPYASMVAIDPSNGYVKAMVGGRDYFATKKDANGRFAKLNLAILGEPGLGPKKSGGT
                     SSGEGRQAGSAFKAFTLAAAIDKGVPLTKTFKGGSCRVFPEISEWATSPGLCNYEGSA
                     YGTVSLLEATIRSINIVFVQLGLDIGMNATTNMAAEMGIHTELLGVPSAPIGANPVNA
                     LGMASGYATLAGNGIYHPPVAITRIYDSSKGKVLYAAPRTKGKEVLSPSSAYIVTSAL
                     EGVISGGTGTAAAIGRPAAGKTGTGQEYRDAWFAGYTPDLAAAVWVGYPEAELPMTST
                     RIGSVTGGSWPAQIWQAFMSPALAGTPPTDFTAPSGLTYVTIDTRSGCVAASGTPSQY
                     QASTPFTPGSEPTNVCVIPDPPDKKKKKGDDGEGRNQDHPGRDDDNGNGNGGNNGGND
                     "
     gene            complement(1877..2479)
                     /locus_tag="VNP73_03310"
     CDS             complement(1877..2479)
                     /locus_tag="VNP73_03310"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWL64980.1"
                     /translation="MKRLPLAFYRRDAVSVARDLIGCIFVHEDIEGRTALRIVEDEAY
                     RGVLDPGSHGYRGLTQRTEVMYGPPGRLYVYFTYGMHWCANVVCAPKGQCEAVLLRAG
                     EPIEGVELMRARRPAATKDTGLAAGPARLTQAMGIGKQHNGSTLLRGGKFFIGMDEKS
                     EAYRAGEIAQTVRVGLGAGKGEEIPWRFVVPGNRFASRPS"
     gene            complement(2476..>3045)
                     /locus_tag="VNP73_03315"
     CDS             complement(2476..>3045)
                     /locus_tag="VNP73_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWL64981.1"
                     /translation="VINLLSDQSHPCQVLADLLTIQEHVTEKLSGTALAYIGDGNNVA
                     HSLLVAGAKMGMDVRVASPRGYQPDAEVVARAREIAAHTEGKVIVSDLVAEAAEGADV
                     LYTDVWASMGQEDESADRANVFEGYRIDESVVAMASPDVKVLHCLPAHRGEEITAGVI
                     DGPMSVVWDQAENRLHTQKALLVWLLSQT"
BASE COUNT          606 a          994 c          926 g          519 t
ORIGIN      
        1 ctcgggagag atccaagagg gtgcctcgac gatcacacag cagtacgtca agcagatgat
       61 catcgcgccc ggcgagatcg cggagaaatc ttacgagcgg aagatccagg aggccgctct
      121 ctcccgccag atcgagaagg aactgagcaa ggaggagatc ctcgagcggt atctgaacac
      181 gatctacctc gggcagggcg cttacggctt ccaggccgcg gccaaggttt acttcgatgt
      241 cggagttacg gatctcaccg tcagccaggg ggcactgatc gcgggcatca tccagcggcc
      301 cggggcgttc gatccattca accacccgag ggccgggaag agacgccgca acctcgttct
      361 cagccggatg cagaacctcg actacatctc cgaggagcgc gccgccaagt taagcaagaa
      421 gcctctcggc cttcagaagg tcaacgagaa ggagagggac caattcgagg ctgcttactt
      481 cgtcgactac gtgcagaaga tgctgacgta tcacccacga ttcgaaatgc tcggtaagac
      541 tgccgcccag aggacgaagg ccctgttcca aggagggctt cgcatcacca cgacagtcga
      601 tctcgacatg cagcgagcgg ccgacaatgc cgtgaactcg atcctctacg acgccagcga
      661 tccttacgcg tcgatggtcg ccatcgatcc ctcaaacgga tacgtgaagg cgatggtagg
      721 aggccgtgat tatttcgcaa cgaagaagga cgctaacggt cgcttcgcaa agctgaacct
      781 cgccattttg ggcgagcctg gactagggcc caagaagagc gggggaacaa gctcgggcga
      841 gggtcgacag gcaggttcag cgttcaaagc gttcacgctc gccgctgcaa tcgacaaggg
      901 agtcccactg acgaagacct tcaagggcgg ctcctgccgg gtcttccccg agatcagcga
      961 gtgggccaca tcccccggac tgtgcaacta cgagggcagc gcctacggca ctgtgtctct
     1021 gctcgaggcg acgatccggt cgatcaacat cgtcttcgtt caattgggcc tcgacatcgg
     1081 aatgaacgcc acgacgaaca tggctgccga gatgggcatc cacacggagc ttcttggagt
     1141 tccatcggct cccatcgggg ccaaccccgt caacgctctc ggtatggcat ccggttacgc
     1201 gacgctcgcc gggaacggca tctaccaccc gcccgtcgcg atcacgagga tctatgactc
     1261 tagtaaggga aaggtgctct acgccgcccc cagaaccaag gggaaggagg tcctcagtcc
     1321 ctcctccgcc tacatcgtca cgagtgccct cgagggagtc atctcgggcg gcaccggcac
     1381 ggcggccgca atcggccgtc cagcggccgg caagaccggc acgggccagg agtaccgcga
     1441 cgcctggttc gccggctaca cccccgacct ggcggcggct gtgtgggttg gctacccgga
     1501 ggcagagctt ccgatgacct cgacccgtat cggatcggtc accggtggat cgtggccggc
     1561 tcagatctgg caggcgttca tgtctcctgc tctcgccggt acgcctccaa ctgatttcac
     1621 ggcgccgagc ggactcacgt acgtgaccat cgacacgcgc tctgggtgcg tcgcagcgag
     1681 cgggaccccg agtcagtacc aagcctcgac tccgttcacg ccgggcagcg agccgacgaa
     1741 cgtctgcgtc attcccgacc cacccgacaa gaaaaagaaa aagggcgacg atggcgaggg
     1801 gcggaaccag gatcacccgg gtcgagacga cgacaatgga aacggcaacg gtggtaacaa
     1861 cggagggaac gactagctag gacggccgcg aggcgaaccg gttgccggga accacgaagc
     1921 gccagggaat ctcctcgccc ttgcccgcac cgagacccac gcgcaccgtc tgagcgatct
     1981 cacccgccct gtaagcctcg cttttctcgt ccatgccgat gaagaacttc ccacctctga
     2041 gcagtgtgga tccgttgtgc tgcttgccga tgcccatcgc ctgtgtgagg cgcgcgggcc
     2101 cagccgccaa gcccgtgtcc ttggttgctg ccggacggcg agcgcgcatg agctccacgc
     2161 cctcgatggg ctcccccgct cttagcagca ctgcttcgca ttgacctttc ggggcacaga
     2221 cgacgttggc gcaccagtgc attccgtagg tgaaatagac gtagaggcgt ccgggggggc
     2281 cgtacatcac ctcggtgcgt tgcgtgagtc cgcggtagcc gtgagacccc gggtctagca
     2341 cgccccggta ggcctcgtcc tccacgatcc tgagcgcggt gcgtccctcg atgtcctcgt
     2401 ggacgaagat gcagccgatg aggtcgcgcg ccacgctcac cgcgtcgcgg cggtaaaagg
     2461 cgagtggcag acgcttcatg tctgcgatag cagccagacg agcagagcct tctgggtgtg
     2521 cagtctgttc tcggcttggt cccacacgac ggacatcggg ccgtcaataa ctcccgccgt
     2581 gatctcctca ccccggtgcg cggggagaca gtgaagaacc ttgacgtctg gcgacgccat
     2641 cgccacgacg ctctcgtcga tccgatagcc ctcgaagacg ttcgcccgat cagcagactc
     2701 gtcctcctgc cccatcgacg cccacacgtc cgtgtacaag acgtcggccc cctccgccgc
     2761 ctccgctact aggtcggaga cgattacctt gccctccgta tgcgccgcga tctcgcgcgc
     2821 gcgtgccacg acttccgcgt ctggctggta gccgcgcgga ctagcgacgc ggacgtccat
     2881 ccccatcttg gctcccgcca ccagcaacga atgggcgaca ttgtttccat caccgatgta
     2941 ggcgagggcc gttccgctca gcttctcggt tacgtgctct tgaatcgtta gcagatcggc
     3001 gagcacctgg cacggatgtg actgatcgct gagcaggttg atgac
//
LOCUS       DATMED010000133         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_479745, whole genome shotgun sequence.
ACCESSION   DATMED010000133 DATMED010000000
VERSION     DATMED010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479745"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VNP73_03320"
     CDS             <1..556
                     /locus_tag="VNP73_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HWL64982.1"
                     /translation="DTMYWDTGLPWVLPSPNMPTVDTALAYPGTGMFEGTNLSEGRGT
                     TRPFELIGAPYIKGALADSLRTKRLKGVQFREAYFIPTFSKYVNETVGGFQTFVTDPH
                     EFDPIHTTLAVIIETKRLYPEDFEWRYDSFDPQRPYWIDKLTGSDHVRTSIDEGQSAD
                     EVVRGWKPELRAFQHTRRRYLLYR"
     gene            573..2300
                     /locus_tag="VNP73_03325"
     CDS             573..2300
                     /locus_tag="VNP73_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019327270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWL64983.1"
                     /translation="MKGSVARVLAAALILTQCVAIASATEGQPGRPMGVDAKELRYGT
                     AQQAGLLPEWIDRVDEEVEAGLQPSPEKPLYPGAVVVAGRNGVIAKHAAYGYAYKYSD
                     DAPTLLPEAERVPMGKETIFDLASVSKLFTSTAVMQLVEEGDIDLWETVASYIPAFGR
                     NGKDSITVEMLLTHTSGLAAWLPLYRDYPTPPERIGAIYDSTPTYPPGTDYVYSDLGM
                     IVLGELVETVSGEPLDEYVHTHITEPLGMTDTGYNPEPSQHDRIAATEYQPWTSRGMI
                     RGSVHDENAWSLGGVAGHAGVFGTGHDLAVLAQTFLNGGRYGRARILDRASVEQMMTN
                     YNTAFPGNDHGLGFELYQHWYMDAMATPTTAGHTGFTGTSMVLNPVDDTFAILLTNRV
                     HPSREWGTINPRRRALARFVGRAVPVHPVAGRDAWFADIGDELDNTLDVPLMLPTGPK
                     RLKLELWYDTEPASDFATVSASGDGGATWNRLEGVLRARGEEPAVTDGTVSGWSEREW
                     LNATFPLDDLNGPVTLRFQYLTDALYGGRGVYVDDVRIKSLRGVLFDDSRRRDRDAFV
                     PDGWLPSRN"
BASE COUNT          458 a          737 c          729 g          422 t
ORIGIN      
        1 cgacaccatg tactgggaca ccgggcttcc ctgggttctt ccttcgccca acatgcctac
       61 ggtcgacacc gcgctcgcct acccgggaac cggaatgttc gaggggacga acctctcgga
      121 gggacgtggg accacccgcc ccttcgagtt gatcggggct ccttacatca aaggtgctct
      181 cgcggacagc ctccgaacca aacgtctgaa gggcgtgcag ttccgagagg cgtacttcat
      241 ccccacgttc agcaagtacg tgaacgagac cgtgggtggc ttccagacct tcgtgaccga
      301 tccacacgaa ttcgacccta tccacacgac gctcgcggtc atcatcgaaa ctaagcgtct
      361 ctacccagag gacttcgagt ggcgctacga ctcgttcgat ccccagcggc cttactggat
      421 cgacaagttg acgggttcgg atcacgtgcg cacgtcgatc gacgaagggc agtcggccga
      481 cgaggtagtt cggggttgga aaccggagtt gcgtgcgttc cagcacacgc ggcgtcgcta
      541 cctgctctat cgctagtcca taggaggaaa tcatgaaggg ctctgtagcg cgggttctgg
      601 cagcggcact gatcctcacg caatgcgttg cgattgccag tgcaactgag ggacaacctg
      661 gtcgaccaat gggagtcgac gcgaaggaac tccgctacgg gaccgcgcaa caggctggcc
      721 ttctccccga gtggatcgat cgggtcgacg aagaggttga ggcgggtctg cagccctcgc
      781 ccgagaaacc gctctacccc ggggcggtgg tggtcgccgg caggaacggc gtcattgcca
      841 agcacgcggc ctatgggtac gcatacaagt actccgacga cgcacccact ctgctgcccg
      901 aagccgagcg agtcccgatg ggcaaggaga cgatcttcga tctagcgtcc gtctccaagc
      961 tgtttacctc gaccgccgtg atgcaactgg tcgaggaggg tgacatcgat ctctgggaga
     1021 cggtggcctc ctacatcccg gccttcggcc gaaacggaaa ggactcgatc acggtcgaga
     1081 tgttgttgac gcacacctcc gggctggccg cctggctgcc attgtaccgg gattacccca
     1141 ccccgccgga gcgcatcggg gctatctacg attcgacccc cacctatccg ccgggcaccg
     1201 actacgtcta cagcgacttg ggaatgatcg tgttgggaga gcttgtcgaa acggtgagcg
     1261 gcgagccgct cgatgaatac gtgcacaccc acatcaccga accgctgggc atgacggaca
     1321 ccggctacaa ccccgagccc tctcaacacg atcgaatcgc cgcaaccgag tatcaaccct
     1381 ggaccagcag gggcatgatc cgaggcagcg ttcacgatga gaacgcctgg tcgcttggag
     1441 gtgttgccgg gcacgcgggg gtgttcggaa cgggtcacga tctggcggtg ctggcgcaga
     1501 ctttcctcaa cggtgggcgg tacggccggg cgcgcatcct cgaccgggcc agcgtcgagc
     1561 agatgatgac caactacaac actgcctttc ccggcaacga ccacggtctc ggcttcgagc
     1621 tttatcaaca ctggtacatg gatgccatgg caacgccgac gacggcgggc cataccgggt
     1681 tcacggggac gtcgatggtg ctcaatcccg tcgacgacac atttgcgatc ctcctcacca
     1741 accgcgtgca cccgagtaga gagtggggga ccatcaaccc ccgcaggcgc gcactggctc
     1801 gattcgtcgg ccgagctgtg cctgttcatc cggtagcggg tcgcgacgct tggttcgcgg
     1861 acatcggcga cgagctcgac aacactctcg atgttccgct gatgcttcct acaggcccga
     1921 agcgattgaa gctcgagctg tggtacgaca ccgaacccgc gagcgacttc gcgaccgttt
     1981 ccgcatcggg cgacggagga gccacctgga accggctgga gggtgtgctc cgagctcgcg
     2041 gtgaggaacc cgcggtgacc gacgggacgg taagcggatg gagtgagcgc gaatggctga
     2101 atgcgacctt tcctctcgac gacctcaacg ggccggtcac gctgcggttc cagtacctca
     2161 cggatgctct ctacggcggt cgcggtgtct acgtcgatga cgtgcgaatc aagagtcttc
     2221 gtggcgtgct attcgacgac tcgaggcgac gcgatcgcga tgctttcgtc ccagacggat
     2281 ggttgcctag tcgtaactaa acagcgcgct ccctcgcgat ccgtgctgcc ccgagtgcag
     2341 ggggag
//
LOCUS       DATMED010000134         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_110940, whole genome shotgun sequence.
ACCESSION   DATMED010000134 DATMED010000000
VERSION     DATMED010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110940"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..799)
                     /locus_tag="VNP73_03330"
     CDS             complement(152..799)
                     /locus_tag="VNP73_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64984.1"
                     /translation="MAMRLPFKRGRTFPLLAVSLGLVVVGAAAQPASAGLGLACPNPT
                     SQAFARWNDRAEYAYAPNGGFENGTGGWKFSGGAKVVAGNETFFIHGAGERSSLSLPA
                     GSSATSPAMCVSLFSTKMRFFARNPGSPSARLRVQVLYNGGVGGLLSILTKTLGLSDV
                     GYVTAGAGWQPSPAIGMLSGTLPLLTQSVQFRFTPADKNGKWQIDDVYLDPFRNR"
     gene            complement(945..2099)
                     /locus_tag="VNP73_03335"
     CDS             complement(945..2099)
                     /locus_tag="VNP73_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008389000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWL64985.1"
                     /translation="MAAERGHEHVETLVIGGGQAGLSMGYELSRRDLPYKIVDANPRI
                     GDVWRNRWDSFRLFTPNRLNRLPGMRFPGYRWGFASKDEWADHLESYARKFDLQVETG
                     VTVEKLTREGDRFIAISGDRRFEADNVVVAMSSWQRPRVPDLAAQLDRRIVQLHVAEY
                     KNPRQLQEGDVLVVGAGNSGAEIAIEVARTHKVMLSGPSTGAIPFRPESVVARVLMPI
                     VGRIIFHRVLTIRTPIGKKVRPKMISSGEPLIRVKPKDLKAAGVERVARVTDVHDGSP
                     QLEDGRAVEVANVVWCTGFHPGFSWIDLPVLGPQEPLHKRGIVGSEPGLYFIGLKFLY
                     SVSSEQIQGVGRDADYIAKKIAAGRKVRQAGRSVGDPSEERDAMASAGGV"
     gene            2193..>2794
                     /locus_tag="VNP73_03340"
     CDS             2193..>2794
                     /locus_tag="VNP73_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWL64986.1"
                     /translation="MEYVKERSDLRLTTTSYAVLAQVAVRPWSTYELVQQRVRYFRYV
                     WPRAESAIYREVKRLSSMGLLAGKTEHVGKRARTVYSITEKGRGALREWLATPVSPFA
                     MDFEAMIRLFVAPLGTKDEIIATLELVLADAREMLRFSGQVKQEFLEGMAVTQDQVYI
                     RVLAVDFFISLLNTVEAWAQRTLDEIESWDDLSPDGKNERA"
BASE COUNT          503 a          877 c          909 g          505 t
ORIGIN      
        1 aaccgtagag atcagcgcca atcgggcgct tgccctcgcc ctgcgcggac cgcgaacggg
       61 attcaaaagg caccacgcga tgctcgcgag cgcctcaaag aatggagccc gggtttcggg
      121 ctccattcct cagtggctgg tgaaggggct gctagcggtt tctgaacgga tcgagataga
      181 cgtcgtcgat ctgccatttc ccgttcttgt cggcgggtgt gaaacgaaac tgcacggact
      241 gggtcaggag cggcagcgtt ccgctgagca tcccgattgc cggcgagggc tgccagccgg
      301 cgccggcggt cacgtacccg acatcggaaa ggccgagagt cttggtgagg atggagagga
      361 ggccgccgac gcctccgttg tagagcacct ggacgcggag cctggccgag ggcgagcccg
      421 ggttccgcgc gaagaaacgc atcttggtcg agaagaggct cacgcacatc gcgggcgatg
      481 tcgcgctact ccccgccggg agcgagagcg aggaacgctc gccggcgccg tggatgaaga
      541 acgtctcgtt gccggcgacc accttcgcgc ctcccgagaa tttccagccg ccggtgccgt
      601 tctcgaagcc gccgttgggt gcgtatgcat actcggcgcg gtcgttccag cgcgcgaaag
      661 cctgcgaggt cgggttcggg cacgcgaggc cgagcccagc actcgccggc tgggcagccg
      721 cgccgacgac gacgagaccg agcgagactg cgagaagcgg gaacgtccgc ccgcgtttga
      781 aggggagccg catagccacc ttcgtcctcc ttgggttgcc gcaggaaatt cgaggcattc
      841 gcctcatcta tcttcatgaa cgatccgcgc ggctttcgcc tcccccgttc gggcatcgat
      901 cgagtcgagc acaaacggag cgagtggtcg ttgaacttct cgcctcacac tcctccggcg
      961 ctcgccatcg cgtctcgctc ctctgacggg tcgcccaccg atcggccagc ctgtcgtacc
     1021 ttgcgcccgg cggcgatctt cttggcgatg tagtccgcgt cgcggccgac gccctggatc
     1081 tgttcggagg aaacggagta gaggaacttg aggccgatga agtagaggcc gggctctgat
     1141 ccgacgatcc ctcgcttgtg caagggctct tgtgggccga ggacggggag gtcgatccaa
     1201 gagaacccgg ggtggaatcc ggtacaccac acgacgttgg cgacctccac ggcgcggccg
     1261 tcctcgagtt gcggcgatcc gtcgtgaacg tcggtgaccc gagcaacgcg ttcgacgcct
     1321 gcggccttga ggtctttggg cttgactcgg atcagaggtt cgccgctaga gatcatcttc
     1381 ggacgaacct tcttcccgat gggagtcctg atcgttagca cccggtggaa gatgatccga
     1441 ccgacgatcg gcatcagaac acgggcgaca acactttcgg gccggaaagg gatcgcgccc
     1501 gtgcttggac ccgagagcat caccttgtgc gtccgtgcca cctcgatggc gatctcggct
     1561 ccggaattgc ctgctcccac gacgaggacg tctccctctt ggagctggcg cgggttcttg
     1621 tactcggcga cgtgaagttg gacgatccgg cgatcgagct gggccgcgag atcgggaacc
     1681 cgtgggcgct gccagctcga catcgcaacc acgacgttgt ctgcttcgaa tcgtcggtcg
     1741 ccggatatgg cgatgaagcg atccccttcc cgagtcagtt tctccaccgt gactcccgtc
     1801 tcgacctgaa ggtcgaactt ccgggcatag gactcgaggt gatcggccca ctcgtccttg
     1861 ctggcgaacc cccagcggta tccggggaag cgcatccccg gaagccggtt gagtcggttg
     1921 ggggtgaaca gccggaatga gtcccagcgg ttgcgccaca cgtcgccgat acgtgggttg
     1981 gcatcgacga tcttgtaggg cagatcgcgc cgagacagct cgtaccccat cgagaggccc
     2041 gcctgcccgc ctccgatcac aagcgtctcg acgtgctcat gtccgcgctc ggccgccata
     2101 tcgccacctc ctcgccccgc cgggctccgt tcggtgaccc ggcgatcaaa gtccagagct
     2161 atactctaat ttggagtatc gtatactcgc gtttggagta tgtcaaggag agatctgacc
     2221 tgagactgac cacgacttct tatgcggtgc tggcccaggt ggcagtgcgt ccctggtcga
     2281 cgtacgagct ggtgcaacag cgggttcgct acttcagata cgtgtggccg cgagccgaaa
     2341 gcgccatcta tcgggaggtg aaacgcctct cttcgatggg cctgctcgca gggaagacgg
     2401 aacacgtcgg caaacgggcg cgcaccgtct actcgatcac cgagaagggg cgaggggcgc
     2461 tacgagaatg gctcgccacg ccggtctccc ctttcgccat ggacttcgag gccatgatcc
     2521 gtctcttcgt agcgccgctg gggaccaagg acgagatcat cgccacgctc gagctggtgc
     2581 tcgccgatgc ccgagagatg ctccggttca gtggacaggt caaacaggag ttcctcgagg
     2641 gcatggcggt cacacaggat caggtctaca tccgcgtgct cgcggtcgac ttcttcatca
     2701 gcctcctcaa cacggtcgag gcgtgggcgc aacggacgct ggacgagatc gaatcgtggg
     2761 acgacctcag ccccgatgga aagaacgagc gagc
//
LOCUS       DATMED010000135         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4323780, whole genome shotgun sequence.
ACCESSION   DATMED010000135 DATMED010000000
VERSION     DATMED010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4323780"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VNP73_03345"
     CDS             complement(<1..548)
                     /locus_tag="VNP73_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWL64987.1"
                     /translation="MQLDRYRRARPHLWRAAIAALVTIAALTAAGTYGQIRKVRGDQV
                     VADTELLIAIAGAGVVLLAGFAAVRAVAAAIRAAVEDERESTKREPLALLVSVVGYVL
                     VLFAVLGALDVPVGKVLLGGALTGVIVGIAAQQSLGNFFAGIILLIIRPFHVGEQVVL
                     KSGPLGGEYEGVITEMSMFYVH"
     gene            627..2018
                     /locus_tag="VNP73_03350"
     CDS             627..2018
                     /locus_tag="VNP73_03350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL64988.1"
                     /translation="MSRVRVIASLAVAVLALPLFVSPAAARSNRDELGFKPPVALFSA
                     SPTRDVASRVYNNGERGKHEWSGEPVIQVDSKGIVYIAGTCCVVASSPVWRSEDGKKF
                     VEMESPGHFREWGVGAEGDLAVDDEGNVFFVDTYVPGMLFTHWSDHGKTWEYTGPTST
                     VPGMNDRPWIAYSNEIMYLYVNHVSHTTVYYSTDQGRTWSFHGPLAWKGEVAGQPYFP
                     GHISADRQTGTLWVGGLNVGEKGNVLGATVSKDGGQTFTEAVVHDPKKGNFFSPIFTG
                     TVAVDDHGTGYVAWSEYNKKGCDVFYASSGNYGKSWSDPVRVSDGKGCATFPWITGGG
                     AGKIGLAWYQTPEVMTPDLTQQFLGMLNGRSYYGAGVPIAPQDEVPPDANWYLHAAAV
                     TGANTNSPDVAEARVPTKTPVAQGPLQRQLWDFLQLDIGPDGRLHITYAEKFKDIAPQ
                     TWYVTNASGPKLN"
     gene            complement(1996..>3110)
                     /locus_tag="VNP73_03355"
     CDS             complement(1996..>3110)
                     /locus_tag="VNP73_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWL64989.1"
                     /translation="GITMTSLGLGAIAEPAVAHLIEGPLHSIVPEEAVHAVALTIALT
                     IVVFFHMVVGEMAPKNIAIAEPEKTALLLALPFRVYANIFRPFIAFLNFLANVGVRLL
                     RVEPQDELKASHSAAEIGMIVHEAAESGVLGRFEQRLLTGAVEFGQRDAASVMVPRTE
                     MVAIPIAATVAEIERVALESGLSRLPVYGKDMDEIIGFFHTKDLLSLPTTERDRPVPR
                     KMIRQMLVVPESLTLHRLLYEMRRQRQHFALVVDEHGGTSGVVTLEDVLEELVGDIRD
                     EYDVAEAGVESFGEGRYVVPGTMHLSEAEDKLGLELPEGEYETIAGFLMDRLGRIPKR
                     RDLVEYTGWRIRVLSMHRRRVVQVLVEKVPSSVSDH"
BASE COUNT          595 a          984 c          983 g          548 t
ORIGIN      
        1 atgtgcacgt agaacatgct catctccgtg atcacgcctt cgtactctcc gcccaatgga
       61 cccgacttca gcacgacctg ttctccgaca tggaacggcc ggatgatgag aaggatgatc
      121 ccggcgaaga aattgcccaa gctctgctgc gcggcgatcc ccacgattac tccggtgagc
      181 gcgccaccta gaagtacttt gccgaccggt acgtcgaggg ctcccaagac tgcgaacagc
      241 accagtacgt agccgaccac cgaaaccagg agggcgagag gctctctctt cgtcgactct
      301 cgctcgtcct ctacggcggc cctgatcgcc gccgctaccg cccgaacggc cgcgaaacct
      361 gccagcagaa cgactccggc tccggcgatc gcgatcagga gctccgtgtc ggccacgacc
      421 tggtcgcctc tgaccttgcg gatctggccg taggtgccgg ccgccgtcag cgcggcgatc
      481 gtcacgaggg cggcgatcgc ggcgcgccac aggtggggtc gggcccgacg gtacctatct
      541 agttgcatgc ggcggaggct aacccggcag ggatatcggg catttccggc gaatctctcc
      601 cctcaggagc caatcaaggg ggatctatga gccgcgtccg ggtcatcgct tcgcttgcgg
      661 ttgccgtgct cgcattgccg ctgttcgtgt cgccggctgc ggcgcgcagc aacagggacg
      721 aactcggatt caagccaccg gtcgcgctgt tctcggcttc gccgacccgg gatgtcgcca
      781 gccgcgtcta caacaacgga gaacggggca agcacgaatg gtcgggcgag cccgtcatcc
      841 aggtcgacag taagggcatc gtctacatcg ccggaacctg ctgcgtcgtt gcgtcgtcac
      901 cggtgtggcg gtcggaagac ggcaagaagt tcgtcgagat ggagtcgccc ggacatttcc
      961 gcgagtgggg cgtgggggcc gaaggcgacc tcgccgtaga cgacgagggg aatgtgtttt
     1021 tcgtcgacac gtacgtaccg gggatgttgt tcacccactg gagcgatcat ggcaagacgt
     1081 gggaatacac ggggccgacg tcgaccgtgc cgggcatgaa cgaccgcccc tggatcgcct
     1141 acagcaacga gatcatgtat ctctacgtga accacgtcag tcacacgacc gtctactact
     1201 cgaccgatca gggccgcacg tggtcgttcc acggccccct cgcatggaag ggcgaggttg
     1261 cggggcagcc gtacttcccc ggccacatct ccgccgaccg ccagaccggg acgctctggg
     1321 tcggtggact gaacgtggga gagaagggca acgtgctcgg cgcgacggtc tctaaggatg
     1381 gcggtcagac gtttaccgaa gcggtcgtgc acgatccgaa gaagggcaat ttcttcagcc
     1441 cgatcttcac cggcacggtt gcggtcgacg atcacggaac gggctacgtc gcctggtccg
     1501 agtacaacaa gaagggctgt gacgtcttct acgcatcctc aggtaactac gggaagtcgt
     1561 ggtcggaccc cgttcgggta tccgacggta agggttgcgc gacgtttccg tggatcaccg
     1621 gtgggggagc aggcaagatc ggactcgcct ggtaccagac tccggaggtc atgacccccg
     1681 acctgaccca gcagttcctc ggcatgctga acggcaggag ctattacggc gccggcgtac
     1741 cgatcgcacc ccaggacgag gttcctcccg acgccaactg gtacctgcac gcggctgcgg
     1801 tgacgggagc gaacacgaac tctcccgacg tggccgaggc tcgagtgccg acgaagacgc
     1861 cggtcgcgca gggaccgctt cagcgtcagt tgtgggactt cctccagctc gacatcggtc
     1921 ccgacggccg gctgcacatc acgtatgccg agaagttcaa ggacatcgcg ccgcagacct
     1981 ggtacgtgac gaacgctagt ggtccgaaac tgaactaggc accttctcga cgagtacctg
     2041 aaccacgcgg cgacggtgca tcgatagaac tcggatgcgc caccctgtgt actcaacgag
     2101 gtcgcgtcgt ttggggatcc gacccagtcg atccatcagg aatccggcga tcgtctcgta
     2161 ctcgccttcg ggaagttcga gacccagttt gtcttcggcc tcggacagat gcatcgtgcc
     2221 gggcacgacg tagcggccct cgccgaagct ttccacgccg gcctcggcga cgtcgtactc
     2281 gtcgcggatg tcgccgacga gctcttcgag cacgtcttcg agagtgacga cccccgaggt
     2341 gcctccatgc tcgtccacga ccaaggcgaa gtgctgacgt tgccgcctca tctcgtagag
     2401 cagacggtga agcgttaacg actcggggac gaccaacatc tgacggatca ttttgcgggg
     2461 gacgggccga tcgcgctcgg tcgtaggcag gctcagcaga tccttggtgt ggaagaaccc
     2521 gatgatctcg tccatgtcct tcccgtagac gggtagccga gacaacccgc tctccaacgc
     2581 aacccgttcg atctcggcga ccgtcgccgc gatcgggatg gccaccatct cggtgcgggg
     2641 caccatcacc gaggcggcgt ctcgttgacc gaactcgact gctccggtga gtagccgctg
     2701 ctcgaagcgg ccgagaacgc cgctctcggc tgcttcatga acgatcatcc cgatctctgc
     2761 agcggaatgg ctcgccttca gctcatcttg gggttcaacg cgcagcaatc gcacgccgac
     2821 gttcgcgagg aagttcagga acgcgatgaa gggccgaaag atgttggcgt agacccggaa
     2881 gggcagggcc agtaacaatg cggtcttctc gggctctgcg atggcgatgt tcttcggtgc
     2941 catctcgccc acgaccatgt ggaagaacac gacgatcgtg agggcgatcg tcagcgcaac
     3001 cgcatgcacg gcctcttcgg gaacgatgga atggagtggg ccttcgatca ggtgcgccac
     3061 cgccggttcg gcgatcgcac caagccccag ggaagtcata gtgataccca
//
LOCUS       DATMED010000136         4327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2418073, whole genome shotgun sequence.
ACCESSION   DATMED010000136 DATMED010000000
VERSION     DATMED010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2418073"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VNP73_03360"
     CDS             complement(<1..761)
                     /locus_tag="VNP73_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL64990.1"
                     /translation="MKRSPVFFVALLIAGLSPAVAYAAPTCDGHKATIVGTDEADEIQ
                     GTDGVDVIASLGGQDTVNALEGDDIVCGGAGDDGLDGGPGNDIVLGGAGSESNQSHVS
                     VDPGGDEEPILLTLDQGFIHGGAGDDVLSGGPGDDDLDGFEGADRLVGGDGNDYLDAG
                     EGDDDLRAGDGDDSSLGGPGDDVAIDTSTNQNYLVGGPGSDKIVTGAGNDDIYGEEGN
                     DIVIAAGGTNYVSGGPGNDRVVAADGDDDIYGGDG"
     gene            complement(758..1186)
                     /locus_tag="VNP73_03365"
     CDS             complement(758..1186)
                     /locus_tag="VNP73_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64991.1"
                     /translation="MDESDDREPFAWVLMDPEQVPERWKSRIRPLAAVPLLPDEAARV
                     LQDGGTSAGVALNEEPFIAMVASGLSPDAIARKTGISLRSVHRRLAALRDDFGVRSTA
                     ELVSQLAARGFGNGTNTPVAPTGEAASAANEFYGDQEGQQ"
     gene            1371..2372
                     /locus_tag="VNP73_03370"
     CDS             1371..2372
                     /locus_tag="VNP73_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWL64992.1"
                     /translation="MEARESRAFESDWPEIARWLKGALARRGVPFGAIDDVVQETGCR
                     LYGMWDRVDATRSTRALALAIANNFMWDEHHRRVSPEPVEDLPEVPTDNDVEAAGIAR
                     LELARVRSALKEMNPSHQAVLLAEIGDAPAPELTPAALKMLRHRARKRLTVILESASA
                     SCAVLGLRLRDFSLRTQHVIRRYSVAFEAPTAGTVAAVAAAAITIGTAATPAVANRQP
                     TTKLVSHTQKAMLVEGRHSDRDGWVHLRKTAMAPTRAKAPRTEYKPQTQQIHLAHQEG
                     PVGTKAAVTWTPTNEGDDTIDPPNCDVTPGAEEVRVRCTADTGDQEYTIDLSTRLRP"
     gene            2418..3239
                     /locus_tag="VNP73_03375"
     CDS             2418..3239
                     /locus_tag="VNP73_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWL64993.1"
                     /translation="MSDIRRGAFFDLDKTLIPGSSLFLLARGLYDRDMFRVRDLLAFG
                     VGQLLFRLRGESARDMTKGRDSTLEFVKGRNSEELIRWGREIATERILPIVYEDIVRV
                     IDGHRDRGDLTFLLTAAPIELAGTLANELMMDGAIGTESEIDDFGRYTGRLVGDIMHG
                     PAKAKAAADLAAEHKLDLAECAAYSDSINDLPLLEAVGYPHAVNPEPELRRIALTRGW
                     PIHELRTRRKALLIGIPAGLGGVGLFGAGVAIGVALERRKWQVGALRRTAQRFRR"
     gene            complement(3236..3412)
                     /locus_tag="VNP73_03380"
     CDS             complement(3236..3412)
                     /locus_tag="VNP73_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64994.1"
                     /translation="MTWILTRVFEVDPSGWSGTIRLPKSPEVAEYAVGAMCELGGHAY
                     YPSSYSVYVGKRSG"
     gene            complement(3801..>4327)
                     /locus_tag="VNP73_03385"
     CDS             complement(3801..>4327)
                     /locus_tag="VNP73_03385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWL64995.1"
                     /translation="AKKDDKREPFERYLADAYAALLSGAGKGRARRPELVVLVSHEVA
                     KRGWKDVKKGEVCKIPGVGPVSPTVAKGIAQDAFLSGLFYDGKDLTLLKRWTRSIPVE
                     VALALELGPPPQFDGVSCVDCGKRFRTEFDHVEPHAAHGMCSTDNMEPRCWSCHRAKT
                     ERDRLDGKLKPAPT"
BASE COUNT          772 a         1424 c         1344 g          787 t
ORIGIN      
        1 ttgccatcgc cgccgtagat gtcgtcgtcg ccatcggccg ccacgactcg gtcgttgccg
       61 gggccgccgc tgacgtagtt ggtccccccg gcagcgatga cgatgtcgtt gccttcttcg
      121 ccgtagatgt cgtcgttgcc ggcgccggta acgatcttgt ccgagcccgg ccctcccacg
      181 aggtagttct ggttcgttga ggtgtcgatg gcgacgtcgt ccccggggcc tccgagactg
      241 ctgtcatcgc cgtcgccggc gcgcaggtca tcgtctccct cgcccgcgtc gaggtagtcg
      301 ttcccgtcgc caccgaccag tcggtcggcg ccctcgaacc cgtcgagatc gtcgtcaccg
      361 gggccaccgg acagcacgtc gtcacccgct cccccatgga tgaaaccctg gtcgagggtc
      421 agcaagatcg gctcttcgtc gccaccgggg tcgaccgaaa cgtgcgactg gttgctctca
      481 ctgccggcac cgcccagaac gatgtcgtta cctgggccgc cgtccaaccc atcgtcgccc
      541 gcgcctccgc aaacgatgtc gtcgccttcg agagcgttga ccgtgtcttg gccgccgaga
      601 ctcgcgatga cgtcgacgcc gtcggttccc tggatctcgt cggcctcatc ggtccccacg
      661 atcgtggcct tgtggccgtc gcaagtgggc gcggcataag cgaccgcggg gctcaaccct
      721 gcgatcagca aggccacaaa gaaaactggg gagcgcttca ttgctgtccc tcctggtctc
      781 catagaattc attcgccgcc gaggcggctt cgcctgtagg ggcaacaggc gtgttcgtgc
      841 cattcccgaa gcccctggcc gccagctgcg acacaagttc ggcggtcgag cgcactccga
      901 agtcgtccct cagagccgcc aggcggcgat ggacgctccg gagcgatatc ccggtcttcc
      961 gggcgatcgc atcgggagag agcccggagg cgaccatcgc tatgaacggc tcttcgttca
     1021 gagccacgcc cgctgaggtg cctccgtcct gcaggacccg ggccgcctcg tccggcagga
     1081 gtggaacggc cgccagaggc cggatccggc tcttccagcg ctccggaacc tgctccgggt
     1141 ccatcagaac ccacgcgaag ggttctcgat cgtccgattc gtccatgttt ccggctcctg
     1201 gcacccatat gccatttttc gacaaaggaa aaagtggtcg ctttgactaa ccattactcc
     1261 gaaaggtcgg cgaggtaacg gtttgaaacg atgcgttacc aaatcgacca atctgcgtac
     1321 ctctggtttg atggccctac aaaggggctg cttggaggac atcagcgggc atggaagcgc
     1381 gggaatcaag ggcattcgag agcgactggc ctgagatagc cagatggctc aaaggcgcat
     1441 tagcacggcg gggcgtcccc ttcggggcga tcgatgacgt ggtccaggag accggttgcc
     1501 ggctctacgg catgtgggac cgggtcgatg cgacacgatc gacacgagca cttgctctag
     1561 ctatcgccaa caacttcatg tgggacgagc atcaccgacg cgtctctccc gaaccggtcg
     1621 aagaccttcc cgaggttccg accgacaacg acgtcgaagc ggccgggatc gctcgtctgg
     1681 aacttgctcg ggtgcgatcc gcactcaaag agatgaaccc ctcccaccag gccgtgttgc
     1741 tcgccgagat cggagacgct ccggcccccg agctgacgcc ggccgcactc aagatgcttc
     1801 gtcaccgcgc gcgcaagcgc ttgacggtca tccttgaatc tgcaagtgca agctgtgcgg
     1861 tgctcggtct tcgtttgcga gacttctctc ttcgcacgca gcacgtgatc cgtcgctact
     1921 cggtggcgtt cgaggctcca accgccggca cggtcgcagc ggtcgccgcg gctgcaatca
     1981 cgatcggaac cgcggcaact cctgcggtag cgaaccgtca gccgacgacc aagcttgtat
     2041 cgcacaccca gaaggcgatg ctcgttgagg gccgccacag cgaccgggac gggtgggtgc
     2101 acctccggaa gacggcgatg gccccgaccc gcgcgaaggc gccgcggacc gagtacaagc
     2161 cgcagacaca gcagatccac ttggcgcacc aggaaggccc ggtcggcacc aaagcggctg
     2221 tgacatggac ccccaccaac gaaggtgacg acacgatcga tcccccgaat tgcgatgtaa
     2281 cccccggtgc cgaagaggtc agggtccgtt gcacggccga taccggtgat caggaataca
     2341 cgatcgatct gagcacgcgt ctccgtccct agcttcgggt acggtcgata gacggccgac
     2401 cgatgcaagg ggcgaccatg agtgacatcc ggcgtggtgc attcttcgac ctcgacaaaa
     2461 cgctgatccc aggctcgagc ctgttcttac tggcgcgggg cctctacgac cgagacatgt
     2521 tcagggtgcg cgatctgctc gccttcggcg tcgggcagct cctgttccgg ctgcgtggcg
     2581 aaagcgcccg cgacatgacc aaggggcgcg attcgacctt ggaattcgtc aagggccgca
     2641 attcggaaga gctgatccgg tggggccgtg agatcgccac cgagcgcatc ctcccgatcg
     2701 tttacgagga catcgtgcga gtgatcgacg gccaccggga ccgaggcgat ctgacattcc
     2761 ttctcaccgc cgctccgatc gagctggccg gaacgctggc gaacgagctg atgatggacg
     2821 gcgcgatcgg caccgaatcc gagatcgacg atttcggccg ctacaccggg cgcctcgtcg
     2881 gcgacatcat gcacggcccg gctaaggcca aggctgcggc cgatctggct gccgaacaca
     2941 agctcgatct ggccgaatgt gccgcatatt ccgactcgat caacgacctc cccctgctcg
     3001 aggccgtggg ctacccgcat gccgtcaatc cggagccaga gttgcgccgg atcgctctta
     3061 cccgagggtg gccgatccac gagcttcgta cccgccgcaa ggccttgctg atcgggatcc
     3121 cggccggttt gggcggggtc gggctgttcg gggccggcgt tgcgatcgga gtggccctcg
     3181 agcgacgcaa gtggcaggtt ggtgcgctga ggcgaaccgc ccaacgcttc cgccgctagc
     3241 cggacctctt gccgacatac acggagtacg acgaggggta gtaggcgtgc ccaccgagct
     3301 cgcacatcgc ccccaccgcg tattccgcta cctccgggct tttcggtagc cggatcgttc
     3361 ccgaccatcc ggacgggtca acctcgaaaa ctcgagtcag gatccaggtc atcttgggat
     3421 cgtgttgacc tggaacaacc tgaacacggg aaagcacgac tatcactgat tcagcggcga
     3481 tcgatgccga ggcgcggcat gccccctccc ctccttcgag cttaagacgc tgccctgcaa
     3541 ccgcccccga gcgagggtag atcctgtagc tacccgtagt tgtgttttta acgaagcgcg
     3601 cgtccgggtc cagcccggta aggcccctaa tggatggagc tcgcacgcgg gagagacgga
     3661 gttcctgttc cccggtatca gttcgaccga gggtcggagt cgttctcgat tcggtgtcgc
     3721 aggcgaagcc cgcgaggtat accagaagca acagacctgc ggcagcctta gtcatcccac
     3781 ccatagcttc gcgccgttag tcaggtcggg gcaggtttca gcttgccgtc gagtcgatcc
     3841 cgttcggttt tagcgcgatg gcagctccaa cagcgcggtt ccatgttgtc ggtagagcac
     3901 atgccgtggg cggcatgcgg ttcgacgtga tcgaactcag ttctgaacct tttgccgcaa
     3961 tcgacgcatg agaccccgtc gaactggggc ggcggtccta actccaaggc gagggcaacc
     4021 tcaacgggga tcgaacgcgt ccaacgcttg aggagcgtga ggtccttgcc gtcgtagaac
     4081 aggccggaca ggaaggcgtc ttgggcgatc cctttggcca ccgtgggtga cactggtccg
     4141 actcccggga tcttgcacac ctcccccttc ttgacatctt tccagccgcg tttggcgact
     4201 tcgtggctga cgagcacaac gagctcaggc cgtctggcac ggcccttgcc cgcaccggac
     4261 agcaatgcgg cgtacgcatc ggcgaggtaa cgctcgaagg gctcgcgctt gtcatccttc
     4321 ttggcct
//
LOCUS       DATMED010000137         9346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3939589, whole genome shotgun sequence.
ACCESSION   DATMED010000137 DATMED010000000
VERSION     DATMED010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3939589"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VNP73_03390"
     CDS             complement(<1..201)
                     /locus_tag="VNP73_03390"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003977330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWL64996.1"
                     /translation="MGRLRYLTAGESHGPALVGILEGLPAGLPVAVKSISDELARRRH
                     GYGRGPRMKIERDALEILSGVRG"
     gene            complement(244..900)
                     /locus_tag="VNP73_03395"
     CDS             complement(244..900)
                     /locus_tag="VNP73_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL64997.1"
                     /translation="MAVPPETIEQPPVDGPPGSNRQRIILVAVLGALVVIALVYFLFL
                     SGGGTEPVESIPPPAAPADSPEPALTDADPDDEPVENFDVFAAKDPFEPAIDAEGEVD
                     EDDDDGDDDEDDEDDSDDGDDDGDDDGDDDSGGGGGGSSGDGGGRSVGGHRVTVVDVY
                     PRGGRAQVQVDNTVWDVREGDRFGDDENFELLSATGECATMLFGDDEFTLCEGEEILK
                     "
     gene            complement(900..1529)
                     /gene="pilO"
                     /locus_tag="VNP73_03400"
     CDS             complement(900..1529)
                     /gene="pilO"
                     /locus_tag="VNP73_03400"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HWL64998.1"
                     /translation="MNARMRMILAIVGIVIIWLVFFFLFIRSRQGELAGIRTEIEAEE
                     SRALQLTTELNRLKELQERAPDLQAELSKIRELVPPEHDVPHFIFLVQDAATESGVEF
                     LNITPELPKTPPEAAPLAQVRMAIGASGSYFSIQDFIRRLHDLDRAVRIDTITMGAED
                     GSLISMDMQTRIFFELPAPPPAETDEAGNVPPPTAPVGSPTPVAPEGGP"
     gene            complement(1526..2245)
                     /locus_tag="VNP73_03405"
     CDS             complement(1526..2245)
                     /locus_tag="VNP73_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWL64999.1"
                     /translation="MMRRVDLLPAIYEQRRRERRNFFLVIIGGLVVLVLLIGWWLVLG
                     FQVGAANEDLADRQARNSELTAEIAELQEFADLETEVQTKTTSLQTVMAGDLDWPALM
                     TEIAMVIPDDVWLTSLSTSAGQTEGAAVVPTETNPIDIDAREPFGRILFDGEALSMRA
                     IANWLIRLETVDEFLAVYLGSASGGEEEATFNFTNTIELGQDAVSGRFQEGVLDEDDD
                     DEDEDEDEDQEDDDEDEDEDE"
     gene            complement(2242..3300)
                     /gene="pilM"
                     /locus_tag="VNP73_03410"
     CDS             complement(2242..3300)
                     /gene="pilM"
                     /locus_tag="VNP73_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HWL65000.1"
                     /translation="MGIGRSAIGLDIGATAVRVAQLAGSRESYSLQRFGQIALPSGSV
                     VDGEIRDPGPVSEAIAQLWKRTKLSHKRVVVGIANQRVVVRQVDFPYLDEAEFRQSLR
                     FQAADYIPMPVDEAELDFMIIDDYTTENQEHMMRVLLVAAATDTVETFVSTVAAAGVQ
                     TVGIDLEPFAIARAVSSSARGELGMDGSEAVIDVGGGVTNVLVHVNGEPRFVRILLVG
                     GDDATQALAEQLSVSAEEAEAVKLDLGREVGPQPAKQILTRQVDALVEEIRGSLDYYL
                     SQEDSEPVTSIILTGGGSLMAGLSQRLEQTLRTPVTPGTPLSQLDVSKSGLTEDQIRQ
                     VEPVSAAAVGLAIGALEK"
     gene            complement(3303..3509)
                     /locus_tag="VNP73_03415"
     CDS             complement(3303..3509)
                     /locus_tag="VNP73_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWL65001.1"
                     /translation="MDVRDFSEPLLTVGEVAQLMRVSNMTVYRLIKSGQLSAIRVGKN
                     YRIRRADVGRYLDERAVHTPGDES"
     gene            complement(3767..5215)
                     /locus_tag="VNP73_03420"
     CDS             complement(3767..5215)
                     /locus_tag="VNP73_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HWL65002.1"
                     /translation="MRRLANQRGVAMVTVLFIGMVLTVVVSSAAFITVREVSSGRDDS
                     RAGRALTYAEAGVDRAYQWLRSNSSGWKYMTLSGCPFSGSTPTASTLRSFSGQLGTGT
                     VNVGTFTSTIQPDDPTNSYCSGTIAPVDTETGPPLDTPYRMKITSTGTYGTTTKVVRQ
                     VVELGVEEFPIGMSANTIDSNGNGTIRNMSVITTSTVTGRDKIDMEGTDFFYTKSDFY
                     PTIAAAQASQLMPASVHTVDRLLGNGGRQAHPPNLNCNFDKQPGDGRGSGWDGSSRGG
                     TISSGTCADANPPPPTSKFTLTDYNRLAVTPRISTDDHLFYKSVAQSAGIYCNIPTSG
                     ATTCTRLGTTDNNIGTNVSTADVTNNPSACPTAPCTFVAYFEFQGGNALNNRVNWDAA
                     TPSTCSAGLVVLIIKNGSTQFGGNARFSGAIFAEDGRVTSNGGPQIEGTVIAKDIQMR
                     GNPIFSMSACWLQNLPGPFLRVTALRWSEADR"
     gene            complement(5212..5871)
                     /locus_tag="VNP73_03425"
     CDS             complement(5212..5871)
                     /locus_tag="VNP73_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWL65003.1"
                     /translation="MNRVRAEAGYTLIEVLIALTVFSIISVGFYQVMVQSVRGSEQSR
                     DVAEISEEARAGFNRMLRDIREADSLDAASSTSFQLWVDYDADSARDYASDEVVVYTF
                     DESAGTVTLASLDSGGSVQTSATLMDGVENDASDTDVFRYTSNRLEYDWSPVDGVTTW
                     EEIDTPQSGVNGIGDRDGTLDVAELSYLSNIIIKFNVQVGDSQTEFLGEGQLRNRRFS
                     V"
     gene            complement(5868..7727)
                     /locus_tag="VNP73_03430"
     CDS             complement(5868..7727)
                     /locus_tag="VNP73_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWL65004.1"
                     /translation="MRRWAWLFVPRRKREQGFSLVETLAALLVFAVITLGLVPLLAAS
                     IRGSDISRRGTVGKNLVTEAMERARGFPFHISYSTQARKVDVLDLYFPSRTPTYAAGT
                     CTGYLNAGSVTCTSTNYTATGPSYVTTCPNASNIGCPQNIPTGYTVTYEASFATVTAG
                     TPETYARFTPATTYRYDLAGSDTPQTLLIQLIVTARWTGTGRGARTYQVRSLLSDRKF
                     SGLKILGDAAVDYGVQLVTGYSDQGPPNKSELTATAGNAVSHIETRRIAAATQSVKAG
                     EVTLIDLVLAEDDIEGNENFDSTPRVGVEETVFAPPDTTVNPTQVTDPADVDHPNNAY
                     GFVASIDTTDSDNVSASVASTLPSAAGEFSFSPSSSEDILIANNRADTSGNPLNLQEA
                     DEWIARVITQGSDALAGNSSCTTNSLGTQPGVACSATTSFSELQLFRMDHIGDEDDIF
                     DGAAVAITGPESGGAPSGVFSATATCNADNDGTQNSTAASATYEGTLWYWSDPTENND
                     DDDGAYVPVPLAPGTDELAAIESGSPILVWDTNPDSNDLYLFPNGSNAGLLANWSNTA
                     SASTSVGTGGRVTSARIENAVSIETQPVEGSSQPETAVAVGIGSVNCRAEDYR"
     gene            complement(7727..8314)
                     /locus_tag="VNP73_03435"
     CDS             complement(7727..8314)
                     /locus_tag="VNP73_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWL65005.1"
                     /translation="MRRLLARPDMAEPVMDKEDGFSLIELLVIMGLVAILMTLGAFAA
                     RQFWFQRSLVGGQDQVVTQLRALQQRVVAEGITTRWYGAWFNTTAATDHTWGIVRYDS
                     VANSCLSLGSFQFDAGVEVSQVDFADTVSGTSVATAITTCRNLSTIPDASDLAFFLSR
                     GTATESTSNLQLNQPRLGRTEEIRILSLTGRAEKI"
     gene            complement(8429..8824)
                     /locus_tag="VNP73_03440"
     CDS             complement(8429..8824)
                     /locus_tag="VNP73_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWL65006.1"
                     /translation="MFQRFHAMKEQREGGFTLIELLVVILIIAILAAIAIPVFLRQRE
                     KGWRSQMESALKNAATAQESYLTANTAYAAAVSDLSNEGLKTAPDVSMAIFANSGTHY
                     CMRATHSSLTSVTYYYSSNSGRPVTSSTC"
     gene            complement(9014..>9346)
                     /locus_tag="VNP73_03445"
     CDS             complement(9014..>9346)
                     /locus_tag="VNP73_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWL65007.1"
                     /translation="GETTGNEVLTRATVEVKDSVRSGETMAAPLGRYDVFPPMVVQMI
                     GVGEDTGALDSMLDKVADFYDQEVTSTTESLTALIEPIMIAVLGGIVGAMVVALYMPM
                     FKIFDLIK"
BASE COUNT         1691 a         2953 c         2835 g         1867 t
ORIGIN      
        1 accccggacg ccggacagga tctcgagagc atctcgctcg atcttcatgc gggggccccg
       61 cccgtaccca tgacgcctcc gcgccagctc gtccgagatc gacttcacgg cgacgggcaa
      121 cccggcgggc aggccctcga ggatgcccac gagtgcgggg ccgtgtgatt caccggcggt
      181 cagataacgg agacgtccca tctaagaatc ctcgcaccga cccgcgtcac gcaagacgcg
      241 gggtcacttc aggatctctt caccctcgca cagagtgaac tcgtcatcac cgaacagcat
      301 ggttgcgcac tcacccgtag cagagagaag ctcgaagttc tcgtcgtcgc cgaacctgtc
      361 gccctcacgc acgtcccaga ccgtgttgtc cacctggacc tgagctcgtc ccccgcgggg
      421 gtacacgtcc accaccgtaa cccggtggcc gccgaccgac cggcccccac cgtcgcccga
      481 ggagcctccc ccaccgccac ccgagtcgtc gtcgccatcg tcgtccccat cgtcgtcgcc
      541 atcgtccgag tcgtcttcgt cgtcttcgtc gtcatcaccg tcgtcgtcgt cttcgtcaac
      601 ctcgccttcg gcgtcgatcg ccggctcgaa cgggtccttc gccgcgaaga cgtcgaagtt
      661 ctcgacgggt tcgtcatccg ggtccgcatc ggtcagagcc ggttccgggg agtcggccgg
      721 agccgccgga ggagggatgg actcgacagg ctccgttccc cctcccgaga ggaacaggaa
      781 gtagacgagc gcgatgacga cgagggctcc cagtacggca acaaggatga tccgttgcct
      841 gttggatccc ggcggcccgt cgaccggcgg ttgttcgatc gtctccggag ggacggccat
      901 cacggacctc cttccggagc gacgggcgtc ggcgagccga ccggcgcggt cggaggcgga
      961 acgttgccgg cctcatccgt ttcggcgggg ggaggtgcag gcagctcgaa gaagatccgg
     1021 gtctgcatgt ccatgctgat cagagaccca tcctctgccc ccatcgtgat cgtatcgata
     1081 cgcacggcgc ggtcgagatc atgcaagcgg cggatgaagt cctgtatcga gaagtaactg
     1141 ccgctcgctc cgatcgccat tcgcacctgg gccaaggggg cggcttcagg aggtgtcttg
     1201 gggagctcgg gcgtgatgtt caggaactcg acccccgact cggtggcggc gtcttgcacg
     1261 aggaagatga aatggggcac gtcgtgctcg ggaggcacga gttcacggat cttggacagt
     1321 tcggcctgca ggtcgggagc gcgctcctgc agctccttca gcctgttgag ctccgtcgtc
     1381 agctggagcg ccctgctctc ctcggcttct atctccgtcc ggataccggc gagctcaccc
     1441 tgacgggagc ggatgaagag gaagaagaac acgagccaga tgatcacgat tcccacgatc
     1501 gcgaggatca tcctcatgcg cgcgttcatt cgtcctcgtc ctcgtcctcg tcgtcgtctt
     1561 cctggtcctc gtcctcgtcc tcgtcctcgt cgtcgtcgtc ttcgtcgagc accccttctt
     1621 ggaaccggcc actgacggcg tcctgaccga gttcgatggt gttcgtgaag ttgaacgtcg
     1681 cttcctcctc accaccgctg gcgctgccca ggtagacggc gaggaactcg tcgaccgtct
     1741 cgaggcgtat caaccagtta gcgatcgccc gcatcgaaag tgcctcgccg tcgaaaagga
     1801 tgcggccgaa aggctcccgg gcatcgatgt caatggggtt ggtctcggtc ggcaccacgg
     1861 ctgcaccctc ggtctgaccg gccgaggtcg atagagacgt cagccatacg tcgtccggga
     1921 tcaccatcgc tatctcggtc atcaaggcgg gccaatcgag atcgccggcc atcacggtct
     1981 gtagggatgt cgtcttcgtt tgcacctcgg tttcgagatc ggcgaactcc tgaagctccg
     2041 cgatctctgc ggtcagctct gagttccggg cttgacgatc ggccagatcc tcgttagccg
     2101 cccccacctg gaagcccaga acgagccacc agccgatgag tagaaccagc accacgagtc
     2161 cgccgatgat caccaagaag aagttgcggc gctcgcgtct gcgctgttcg tatatcgccg
     2221 gaaggagatc gaccctgcgc atcatttctc gagggcccca atcgcgagtc cgacggcagc
     2281 cgcagagacg ggctcgacct gacggatctg atcttcggtt agcccggact tcgatacgtc
     2341 aagctgggac aggggtgtgc ccggagtcac cggcgtgcgc agggtttgct ccaagcgctg
     2401 cgaaaggccg gccatcaacg atcccccacc cgtcaagatg atggaagtga ccggctcgct
     2461 gtcttcttgc gacaagtagt agtcaaggga gccgcggatc tcttcgacga gtgcatcgac
     2521 ctgcctcgtg aggatctgct tggcgggttg cgggccgacc tcacgaccga gatcaagttt
     2581 cacggcctcg gcctcctcgg cggatacaga aagctgttcg gcgagagcct gagtcgcgtc
     2641 gtctccgccc accagcagga tcctgacgaa gcgtggctct ccgttcacgt ggacgagcac
     2701 gttcgtcacg ccgccaccga cgtcgatgac cgcctcagag ccgtccatcc ctagttcgcc
     2761 ccgagcggag ctgctgaccg cgcgtgcgat ggcgaacggt tcgagatcga tgccgacggt
     2821 ttggaccccg gccgcggcaa ccgtggatac gaaggtctcg acggtgtcgg ttgcggcggc
     2881 gaccagcagg acccgcatca tgtgctcctg gttctcggtc gtgtagtcgt cgatgatcat
     2941 gaaatcgagc tcggcttcat caaccggcat cgggatgtag tccgccgcct ggaaccgcag
     3001 ggactgcctg aactcggctt cgtccaggta ggggaagtcc acctgacgaa cgaccacgcg
     3061 ctggttggcg atgccgacga cgactctctt gtgagagagc ttcgttctct tccagagttg
     3121 agcgatcgct tcggagaccg gaccggggtc tcggatctca ccgtccacga cggaccccga
     3181 cgggagagcg atctgaccga accgctggag cgagtaggac tcgcgcgatc cggcaagctg
     3241 cgcgacccgc accgcggtcg ccccgatgtc gagaccgatc gcactccgtc cgatccccat
     3301 cttcacgatt cgtctcccgg cgtgtgaacg gcgcgttcat cgagatagcg cccgacgtcg
     3361 gcgcggcgta tgcggtagtt cttgcccacc cggatcgcgg atagttggcc ggatttgatc
     3421 aggcggtaga cggtcatgtt cgagacgcgc atcagctgcg cgacctcccc caccgtcagg
     3481 agaggttccg agaaatccct tacgtccacc tcagtcgtcg cccttctaac gggcgcaccc
     3541 catcggcgcg cacgcgcgta gacccccggc atgagggcaa ctcatttaac aatcgttgac
     3601 aattcaattc aagcgtataa agagcgctca atacttgaca aattacgggc gatttgccct
     3661 tttcgcggcc ttgtggggaa gaaaccgccg gaaggatatg ccgcgcgtcg attcggcgct
     3721 agccgcgcct atccgaggga gttacagggg gccgagcttc cagttcctag cggtctgctt
     3781 cgctccagcg cagggcggtg accctcagga aggggccggg gaggttctgg agccagcagg
     3841 cggacatgga gaagatgggg ttccctcgca tctggatgtc cttggcgatc acggtcccct
     3901 cgatctgcgg cccaccattc gaggtgacgc gcccatcctc ggcgaagatc gctccggaaa
     3961 agcgagcatt accgccaaac tgggtgcttc cgttcttgat tatcaacacg accaggccgg
     4021 cgctacaggt gctcggcgtc gccgcgtccc agttcaccct gttgtttaga gcgttcccac
     4081 cttggaactc gaagtaggca acgaacgtgc acggtgccgt gggacacgcg ctcgggttgt
     4141 tcgttacatc ggcggtgctg acgttcgttc cgatgttgtt gtcggtcgtt cccaaccggg
     4201 tgcacgttgt ggcgccactc gtcgggatat tgcaatagat cccggccgac tgagcaaccg
     4261 acttatagaa gagatggtca tcggtagata tccggggtgt tacagcaagt ctgttgtaat
     4321 cggtaagcgt gaacttcgag gtgggcggcg gcgggttcgc gtcggcacag gttcccgacg
     4381 agatcgtgcc tcccctcgag ctcccgtccc aaccggaccc ccgcccgtca ccgggctgct
     4441 tgtcaaagtt gcaattgagg ttagggggat gagcttgtcg cccgccgttg cccagaaggc
     4501 ggtcgaccgt gtgaaccgac gccggcatca actgcgacgc ttgggccgcc gcgatcgtgg
     4561 gatagaaatc gctcttcgta tagaagaaat cggttccctc catatcgatc ttgtcccgcc
     4621 ccgtcacggt gctggtcgtg atcacgctca tgtttcggat cgttccgttc ccgttcgagt
     4681 cgatcgtatt ggcggacatc ccgatcggga actcttccac cccaagctct acaacttggc
     4741 gaacgacctt cgtggtggtt ccgtatgttc cggtcgaggt gatcttcatc ctgtaggggg
     4801 tatccagtgg cggtccggtt tcggtatcaa ccggagcgat ggtcccggag caatagctgt
     4861 tggtcgggtc gtcgggctgg atggtagatg tgaaggtccc cacattcacc gttccggtcc
     4921 ccagctgtcc ggagaaactg cgcagtgtgg atgcggtcgg cgtggacccg gagaacgggc
     4981 accccgaaag cgtcatgtat ttccatccgg agctgttact ccggagccat tggtaggcac
     5041 ggtcgacccc ggcttcggcg taggtgagag cccggcccgc gcgcgagtcg tcgcgacctg
     5101 aggagacctc tctcaccgtg atgaacgccg cgctcgagac gacgaccgtc aagaccatgc
     5161 cgatgaacag cacggttacc atcgcaacac cacgttggtt cgccaaacgc ctcatacaga
     5221 gaatctccgg ttcctcaatt gaccttcacc taagaattcg gtctgcgagt cccccacctg
     5281 aacgttgaac ttgatgatga tgttgctcag ataggacaat tcggccacgt ccagcgtgcc
     5341 gtcccggtcg ccgatcccat tcacgcccga ttgaggagtg tcgatctctt cccacgtcgt
     5401 gaccccgtcg accgggctcc agtcgtactc gaggcgattg ctggtgtagc ggaagacatc
     5461 ggtatcggat gcgtcgttct cgacgccgtc catcagtgtc gccgacgtct gaacgctgcc
     5521 gccgctatcc agcgaagcca gcgtgactgt gccggcactc tcgtcgaacg tgtacacgac
     5581 cacctcgtcc gacgcgtagt ccctcgccga atcggcgtcg tagtcgaccc agagctggaa
     5641 cgaggtcgag ctcgcggcgt cgagcgaatc ggcttcccgg atgtcccgga gcatgcgatt
     5701 gaacccggcc cgggcctcct cggatatctc ggcgacatct cggctctgtt ccgatccacg
     5761 gacgctttga accatcacct ggtagaagcc cacggagatg atcgagaaga cggtcagcgc
     5821 gatcagaacc tcgatcaacg tgtagccggc ctcggcccgg acccggttca tcggtaatcc
     5881 tccgcccggc agttgacgga acctatcccg accgccacag cggtttcggg ctggctcgag
     5941 ccttctacag gttgcgtctc gatgctcacc gcgttctcga tccgagcgct ggtgacgcga
     6001 ccacccgttc cgacgctcgt gctggcgctc gccgtgttgg accagttggc gagcaggccg
     6061 gcgttgctgc cgttggggaa cagatagagg tcgttggagt cggggttcgt gtcccagaca
     6121 agtatgggtg agccgctctc gatcgcagca agctcatcgg ttcccggggc cagcgggacc
     6181 ggcacgtagg caccgtcgtc gtcgtcgttg ttctcggtcg gatcgctcca gtaccacagc
     6241 gttccctcat aggtagccga ggcggcggtg gagttctggg tcccatcgtt gtcggcgttg
     6301 cacgtggcag tagcagagaa gacgcccgag ggcgctcccc cactttcggg cccggtgatc
     6361 gctaccgcgg cgccgtcgaa tatgtcgtct tcatctccga tgtgatccat cctgaacaat
     6421 tgcagttcgg agaaactcgt cgtcgccgaa caggcgacac ccggctgcgt cccgagggag
     6481 ttcgtcgtgc acgatgagtt tcccgcgagc gcgtcgcttc cctgtgtgat gacccgagct
     6541 atccattcgt cggcttcctg gaggttcagg gggttgccgc tcgtgtcggc gcgattgttg
     6601 gcgatcagga tgtcttctga ggacgacggg ctgaaggaga attcaccggc agcgctaggc
     6661 aacgtgcttg ctacgctcgc gctcacgttg tccgaatccg tcgtatcgat cgatgcaacg
     6721 aacccgtagg cgttgttggg atgatcgacg tcggccgggt cggttacctg agtcgggttc
     6781 acggtggtgt cgggtggagc gaacaccgtc tcttcgacac ccacgcgagg ggtgctgtcg
     6841 aaattctcgt tgccctcgat atcgtcttca gccagtacca gatcgatgag ggtgacctcc
     6901 ccggctttga cgctctgggt cgccgccgcg atccgcctcg tctcgatgtg ggagacggca
     6961 ttaccggccg tcgcggtcag ctcggacttg ttggggggac cctggtccga ataccccgtc
     7021 accagctgca cgccgtagtc caccgcggcg tcgccgagga tcttcaggcc cgagaacttc
     7081 cgatcgctca gcaaggagcg aacctggtag gtacgggcac cgcgaccggt tccggtccac
     7141 cgagcagtaa cgatcaactg gatcaacaga gtctgcggcg tgtcgctgcc ggccaggtcg
     7201 tatcggtacg tcgtggcggg cgtgaaacgc gcgtacgtct cgggtgtccc cgccgtcacc
     7261 gtggcgaacg atgcctcgta cgtcaccgtg tatccggtgg ggatgttctg tgggcacccg
     7321 atgttcgacg cgttggggca tgtggtcacg tagctcgggc cggtcgcggt gtagttagtg
     7381 gaggtgcaag tcacacttcc ggcgttgaga tacccggtgc atgtacccgc tgcgtaggtc
     7441 ggcgtccggc tcggaaagta gaggtcgagc acgtccacct tccgtgcttg cgtcgaatag
     7501 gagatgtgga atgggaagcc gcgcgctcgc tccatcgctt cagttacgag gttcttaccg
     7561 acggtacccc ggcgcgagat gtccgaccct cggatcgaag cagctagtag cggcacaaga
     7621 cccaacgtga tgactgcgaa cacgagtagc gcggccaagg tttcgacgag cgagaaacct
     7681 tgttcccgct tgcgccgagg cacgaacaac cacgcccacc gtctcatcag atcttctccg
     7741 cccggcccgt cagactcagg atgcggatct cttccgtccg gccgaggcgc ggttgattca
     7801 gctgcagatt cgaggtcgat tcggttgccg tcccccggct gaggaagaaa gccaggtccg
     7861 acgcgtccgg gatcgtcgac aggttgcgac aggtcgtgat cgctgtagcc acactggttc
     7921 cggaaacggt atcggcgaag tccacctgac tgacctcaac gccggcatcg aactggaagg
     7981 acccaagtga caagcaggag ttcgcgaccg agtcgtagcg aacgatcccc cacgtgtggt
     8041 ccgtcgccgc agtcgtgttg aaccaggcgc cataccagcg tgtcgtgatc ccctccgcca
     8101 ccactctctg ttgcagagct cggagttgag tcacgacctg gtcctggccc ccaaccaggg
     8161 atctctggaa ccagaactgg cgagcggcga acgcgcccaa tgtcatgagg atcgccacga
     8221 gccccatgat cacgagcaat tcgatcagtg aaaaaccgtc ctctttatcc attaccggct
     8281 ctgccatatc cgggcgcgct agaaggcgcc tcacctatag acaatcggct atctaaggat
     8341 gcaccttgaa gcccgataag cgaagtattc ccagacatga aaaagggcgg ggcctgagcc
     8401 ccgcccttgg tcttaactgg tggtgcctct agcaggtcga gctcgttacc ggccgaccag
     8461 agttgctgct gtagtagtac gtcaccgaag tcaggctcga gtgggtcgcc ctcatgcagt
     8521 agtgcgtacc actgttggcg aagatcgcca tcgacacgtc cggcgccgtc ttcagaccct
     8581 cgttagaaag atccgatacg gccgccgcat aggccgtgtt ggcggtcagg taggactctt
     8641 gagcggtagc cgcgttctta agcgcagact ccatctgcga tctccagccc ttttccctct
     8701 ggcgcaagaa aacggggata gcgatcgcgg caaggattgc gatgataagg atcacgacca
     8761 acagctcgat aagggtgaaa ccgccttcac gctgctcctt catagcgtgg aaccgctgga
     8821 acatcagttc tcctcccttc tgtccctggc agtccaccta gggacaacgt gtttgaaagt
     8881 atccatcact cccatttgga gtcaaggact ctcctctttc tggtactgat atcggcactg
     8941 gggctcgttt ctttatctag gcacatatgc ctacccgagc ttcccttctg ggtagttccg
     9001 ccccccgtaa gggtcatttg atgaggtcga agatcttgaa catcggcatg taaagagcca
     9061 cgaccatagc tccgactatc ccgcccagca ccgcgatcat gatcggctcg atcaaggcgg
     9121 taagtgactc ggtggtagac gtgacttcct gatcgtagaa gtcggccacc ttgtcaagca
     9181 tggaatcgag agcaccggtg tcctcaccga cgccgatcat ctgtacaacc atcggcggga
     9241 acacatcgta acgccccagc ggcgcggcca tcgtctcacc gcttctgacg ctgtctttca
     9301 cctcaaccgt tgcccttgtc agcacttcgt tacccgtcgt ctctcc
//
LOCUS       DATMED010000138         2874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4714097, whole genome shotgun sequence.
ACCESSION   DATMED010000138 DATMED010000000
VERSION     DATMED010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4714097"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1023)
                     /locus_tag="VNP73_03450"
     CDS             complement(247..1023)
                     /locus_tag="VNP73_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWL65008.1"
                     /translation="MSRLQNKACVVTGAGRGIGRAVALAFAAEGAQVLVNDVDAQNAA
                     SVVKEIVEAGGSAAPDTNAIGTVEAAQSLVENALDAFGQVDVLVNNAGILRDRMLHNM
                     TEEDFDQVIQVHLKGTWACGRAVVQHWRPIAKKEAEAGTPRHRKIINVTSPSGLTGAA
                     GQSNYAAAKMGIVGLTKTWAKELGRLAINSNAISPAALTAMTEPLVQTEEDKAKRHAR
                     FVLGRYPMPEEIAPTVVFLASDDSNWMTGQVLNIDGGLVI"
     gene            1048..1524
                     /gene="greA"
                     /locus_tag="VNP73_03455"
     CDS             1048..1524
                     /gene="greA"
                     /locus_tag="VNP73_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWL65009.1"
                     /translation="MTEEKKAPLTQDAFERVQERLVFLEGEDRQRIINDIAVARAHGD
                     LSENAEYHAAKDEQGQKEAEIRKLRQMVENAEIVSGDDSGVVKPGMLVTIKHAGDTEA
                     ETYLLGLREEKGGDYDVLTPDSPLGQALIGKSSGETVVAKVPAGEIKVEVLEVRAL"
     gene            complement(1521..2585)
                     /locus_tag="VNP73_03460"
     CDS             complement(1521..2585)
                     /locus_tag="VNP73_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWL65010.1"
                     /translation="MNPDCVHASTALLGPGLDAADGVSIRIEDGTISDIEVGVDPGAD
                     DLDLDGITLLPGFIDTHVHIGFWPPIEVLVGGVTTVRDLAWPPEQIYPLVHRSRSPDF
                     EGPEIVAAGPMLTCPAGYPVAAAWAPPGTARIVTDVDDARSAVRERVAEGAVIVKVAL
                     NPAVGPTLSEEVLQAICDEAHSCGLEVTGHVFGIGELEKAIRCGMDELAHMLMSSDRI
                     ADSTIDEMVARGMRIVPTLAIHRSRTKRATIINLSRFVHAGGRIIYGTDLGNAGVHPG
                     IEPNEIDRMARSEMSPTAILASATVEAAAALRLKDRGVLASGYRADIIGVRGDPTDAP
                     IVLKDLVFVMRGGIVRRLDT"
     gene            2630..>2874
                     /locus_tag="VNP73_03465"
     CDS             2630..>2874
                     /locus_tag="VNP73_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65011.1"
                     /translation="MEPGDIIWEGKIGKTSLRVGVPHAEMIIAEWDSSRGPRHKISDS
                     IEEFERSVLFQLLLESGTAEDPATQEVLGAIQIAQAER"
BASE COUNT          534 a          907 c          921 g          512 t
ORIGIN      
        1 aagccggacg gcgctacgac gaccagttgc gcgtcgtgaa gcccgatgcg gataacgacc
       61 gcctcgactt cgttgccgta ggtctcgacg aattcgtcgg gtcgattgaa cttgcgtctc
      121 tcggttacgc cctcgaaggg ccatgcgcgt gccacggatc gggagtctag gccaccgacc
      181 gacaggcccg gcgttgggca gtgggcgggc ggtgctgatt gggcgggcga gagcccgcac
      241 cgctattcag atgaccaggc ctccgtcgat gttcaagacc tgccccgtca tccagttgga
      301 atcgtctgat gccaggaaca cgacggtcgg agcgatctcc tcgggcatcg ggtatcggcc
      361 cagcacgaag cgggcatgtc gcttggcctt gtcctcctcg gtctggacga gcggttcggt
      421 catcgccgtg agagctgcgg gggagatcgc gttcgagttg atcgccaagc gtccgagttc
      481 cttcgcccac gttttggtca gacccacgat ccccatcttt gcggccgcgt agttcgactg
      541 cccggcggct ccggtgagac ccgacggaga cgtgacgttg atgatcttgc ggtggcgagg
      601 cgtcccggcc tcggcctcct tcttggctat cggcctccag tgctgcacga ccgcccgtcc
      661 gcatgcccag gtccccttca gatggacctg gatcacttgg tcgaagtctt cctcggtcat
      721 gttgtgcagc attcgatcgc ggaggatgcc ggcattgttc acgagcacgt ccacctgacc
      781 gaaggcgtcg agcgcgttct cgaccagcga ctgggctgcc tcgaccgtgc cgatcgcgtt
      841 cgtgtcgggc gccgcactgc ccccggcttc cacgatctcc ttgacgacgg aagccgcgtt
      901 ctgtgcatcg acatcgttga cgaggacctg cgcgccttcc gccgcgaagg ccaaggcgac
      961 tgcccgtccg atacctctcc cggcccccgt aacgacgcaa gctttgtttt ggagtcgaga
     1021 catgtgcgaa tagtagtctg accacgcatg actgaagaga agaaggctcc gttaactcag
     1081 gacgcgttcg agcgtgtcca agagaggctg gtcttcctcg aaggcgagga caggcagcgg
     1141 atcatcaacg acatcgcagt cgctcgagcc cacggcgacc tcagcgagaa cgcggagtac
     1201 cacgcggcta aggacgagca aggacagaag gaagcagaga tccgcaagct ccggcagatg
     1261 gtcgagaacg cggagatcgt ctccggcgat gacagcggtg tggtcaagcc cgggatgctc
     1321 gtgacgatca agcacgctgg tgacaccgag gccgagactt atctgctcgg cctgcgagaa
     1381 gagaagggcg gggactacga cgtgctcacc cccgactccc cgctggggca ggccctgatc
     1441 ggcaagtcgt cgggcgaaac ggtggtggcc aaggttccgg caggcgagat caaggtcgag
     1501 gtgctcgagg tgcgcgcact ctaggtatcg agcctgcgaa cgatcccacc tcgcatcacg
     1561 aaaacgagat ccttcaacac gatcggggca tcggtcggat cgcctcgaac tccgatgatg
     1621 tctgcgcgat agcccgacgc gagtactccg cggtccttca atctcagcgc agccgcggcc
     1681 tcaacagtcg ccgaagcaag gatcgcggtg gggctcatct ccgatcgcgc catccgatcg
     1741 atctcgttcg gttcgatccc cggatgcact cccgcgtttc ccagatccgt tccgtagatg
     1801 atccggccgc cagcgtgcac gaacctactc aggttgataa tcgtcgctcg tttggttcgg
     1861 gatcgatgga tcgcaagagt gggcactatg cgcatgccgc gcgcgaccat ctcgtcgatg
     1921 gtgctatcgg cgatccgatc ggagctcatc agcatgtgtg cgagctcgtc catcccacat
     1981 cggatcgcct tctccaattc gccgatgccg aacacatgcc cggtgacctc caggccgcag
     2041 gaatgtgctt cgtcgcagat cgcttggagg acctcctcgg acaatgtagg cccgacggcg
     2101 gggttgagcg caaccttgac gatcaccgct ccctccgcga cgcgttcccg gacggcagaa
     2161 cgcgcatcgt ctacatcggt cacgatccga gccgtacccg ggggagccca cgctgcggcg
     2221 acgggatagc cggcggggca cgtgagcatc gggcctgcgg cgacgatctc gggcccctcg
     2281 aaatcgggcg agcgcgacct gtggaccaac ggatagatct gctccggcgg ccacgccagg
     2341 tcgcgaaccg tggtgacgcc tccgacgagc acctcgatcg ggggccagaa gccgatgtgg
     2401 acgtgggtat cgatgaagcc cgggaggagc gtgatcccgt cgagatcgag gtcgtcagcc
     2461 ccaggatcga cgccgacctc gatgtcggag atcgttccat cctcgatccg gatcgaaacg
     2521 ccgtcggccg catccaggcc tggtccgagg agtgcggtgg atgcgtgaac gcagtctgga
     2581 ttcatcatcc ggtaacttag gacgaccgac taccgatgga gatcactgag tggaacccgg
     2641 cgacatcatc tgggaaggca agatcggcaa gacctcactg agggtggggg tgccccacgc
     2701 cgagatgatc atcgccgagt gggactcatc ccgagggccc cgacacaaga tctccgactc
     2761 gatcgaggag ttcgagaggt cggtgttgtt ccagctcctg ctcgagtccg gcaccgccga
     2821 ggatccggcc acccaagagg tcctgggtgc gatccagatc gctcaagcgg aacg
//
LOCUS       DATMED010000139         2851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1254619, whole genome shotgun sequence.
ACCESSION   DATMED010000139 DATMED010000000
VERSION     DATMED010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254619"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VNP73_03470"
     CDS             complement(<1..435)
                     /locus_tag="VNP73_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWL65012.1"
                     /translation="MTSDRHPTERTRVRRLPARGAYDRDTIYRILDEALICHVGFVVD
                     GSPYVIPMVAARAGDSLLLHGSTASRLTRHLAGGAEVCVTVTHVDGLIVSRSVFDSSM
                     NYRSVVILGSTQPITEQDAKLEAMRAITEHLLPGRWDEARHPN"
     gene            473..1540
                     /locus_tag="VNP73_03475"
     CDS             473..1540
                     /locus_tag="VNP73_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HWL65013.1"
                     /translation="MTTVTLIPGDGIGPEVTAATRRVVDALGAGIEWDEHRAGAATLS
                     SHGTPLPSHVLDSIRHNKVALKGPVTTPVGTGFRSINVALRKELDLFASVRPCKLYAG
                     VASRFSDIDLVVVRENTEDLYEGIEFAQGSKEVNKLRGYLKYMHGFTMPRDAGVSVKP
                     ISITATRQIVKYAFEYAKRYRRRRVTLGHKANIMKFSDGLWLETGEILAEGYREVGFE
                     AIQIDELAMWLVYHPDDLDVIVLSNLYGDIISDLCAGLIGGLGMAPGMNLGTDYAVFE
                     PTHGSAPDIAGAGLANPFAMMLSAVLMLRHLGLRDEGDRLETAIARVLRLGKVRTGDL
                     RPKGEPATTPEVADAVIEALA"
     gene            1550..2338
                     /locus_tag="VNP73_03480"
     CDS             1550..2338
                     /locus_tag="VNP73_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL65014.1"
                     /translation="MGTEGTLTDRDVLARVAAFADEHQNRSASRRESWSGGSAIFHDD
                     LPLIYDLNLMRLEVEGLDVATVAAEADRLMGQLGHRQLTVRDEVVGRTLVDGLVERGW
                     HADRHVVMVHRREPDRVVDTHDVEEVAEPVVWPSRAEWLRTYEWASDKATIDQLHAAY
                     RIWMRAGNGRDFALKRDDKPVSFAMLWTEGEVAQIEDVATLDSHRNQGLCRAVVGRCV
                     EEARSQGCDVIFLIADEADWPKELYRKIGFETVGCFYYFLKTNS"
     gene            complement(2314..2757)
                     /locus_tag="VNP73_03485"
     CDS             complement(2314..2757)
                     /locus_tag="VNP73_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65015.1"
                     /translation="MRRYLAALVVALLVAIPTAAPAGPKKVSGSFGASLAPFPNYSSH
                     TSTARPGCAAGQENVHWVGQEFSAPAAGTLSMHSEGFTGDHDIYVFVNDIPYARGENT
                     QVPDGAPPEESVTVPLKAKQKVQMVACNWLGQPEVEAHYEFVFKK"
BASE COUNT          526 a          913 c          919 g          493 t
ORIGIN      
        1 gttggggtgc cgcgcctcat cccaccgacc gggcaacagg tgctctgtga tggcgcgcat
       61 cgcctccagc ttcgcgtctt gttccgtgat cggctgcgtg ctccccagga tcaccaccga
      121 gcgatagttc atggaactgt cgaacaccga gcgcgacacg atcaatccat ccacatgcgt
      181 caccgtgacg cacacctcgg cccccccggc gagatgacgc gtcaagcggc tggccgtcga
      241 tccgtgtaag agcaggctgt ccccggcacg cgccgccacc atcgggatga cgtaaggcga
      301 accgtcgacc acgaacccga cgtgacagat cagcgcttcg tccaggatcc gatagatcgt
      361 gtctcggtcg taagcacctc gggccggcag tctccgcacg cgggttcgtt ccgtgggatg
      421 gcggtcgcta gtcatcccgg cattctcctc cgcccctcgt aaggttagag gcatgacgac
      481 cgtgacactg atcccgggag acgggatcgg gcccgaggtg accgccgcga cccgccgggt
      541 cgtcgatgcc ctgggggccg ggatcgagtg ggacgagcac cgcgccggtg cggcgaccct
      601 gtcgtcgcac ggaaccccgc tcccctccca cgtcctcgat tcgattcgtc acaacaaggt
      661 cgcattgaag ggcccggtga ctactccggt gggtacgggc ttccgctcga tcaacgtcgc
      721 cctcaggaaa gagctagacc tgttcgcaag cgtgcggccc tgcaagctct acgccggtgt
      781 tgcgtcccgc ttctccgaca tcgatcttgt ggtcgtgcgc gagaacacag aagacctcta
      841 cgaggggatc gagttcgccc agggctcgaa agaagtaaac aagctgcgcg gctatctcaa
      901 gtacatgcat gggttcacga tgccgcgcga tgccggggtg tcggtgaaac cgatctcgat
      961 caccgcgact cgccagatcg tgaaatacgc cttcgagtac gcgaagcgat accggcgtcg
     1021 acgcgtgacg ctcgggcaca aagcgaacat catgaagttc tccgacggcc tgtggctgga
     1081 gaccggagag atcctcgccg agggctatcg tgaggtcggc ttcgaagcga tccagatcga
     1141 cgagcttgcg atgtggctcg tgtaccaccc cgacgatctc gacgtgatcg tgctctcgaa
     1201 cctctacggc gacatcatct cggacctatg cgcgggcctc atcgggggcc tcggcatggc
     1261 accgggcatg aacctcggca cggattacgc ggtgttcgag ccgacgcacg gatccgcgcc
     1321 cgacatcgcg ggggccggcc tcgccaaccc gttcgccatg atgctgtccg ccgtgttgat
     1381 gttgaggcat ctcgggcttc gcgacgaagg agatcgtctc gaaaccgcga tcgctcgggt
     1441 gctcaggctc ggcaaggtcc gaacgggcga ccttcgtccc aaaggcgagc cggccacgac
     1501 acccgaggtc gccgacgcgg ttatcgaagc actagcctga tcaccgaaca tgggaaccga
     1561 gggcaccctg accgaccgcg acgtcctggc gcgggtcgcg gcgtttgccg acgaacacca
     1621 gaaccggagc gcgtcgcgtc gcgagtcgtg gagcggaggc tccgccatct tccacgacga
     1681 cctgccactg atctacgacc tgaacctgat gaggctcgag gtcgaaggcc tcgatgttgc
     1741 gacggtcgcc gccgaagccg atcgcctgat ggggcaactg ggccatcgcc agttgaccgt
     1801 gcgcgacgag gtggtgggtc gcacgctggt ggatgggctc gtcgagcgcg gctggcacgc
     1861 agaccgccac gtcgtgatgg ttcatcgtcg cgagccggat cgtgttgtcg atacccacga
     1921 cgtcgaagag gtggccgagc cggtggtgtg gccgtcacgc gccgaatggc tacgcaccta
     1981 cgaatgggca agcgataagg caacgatcga ccagctgcac gcggcgtatc ggatctggat
     2041 gcgcgccggc aacggccggg acttcgccct caagcgagac gacaagccgg tgtcgttcgc
     2101 gatgttgtgg accgagggag aggtcgcgca gatcgaagac gtcgcaaccc tcgattcaca
     2161 tcgcaaccag gggctatgcc gagcggtcgt cggacgatgc gtagaggaag cccgttctca
     2221 aggatgcgac gtgatcttcc tcatcgcgga cgaagccgac tggccgaagg agctctacag
     2281 aaagatcggg ttcgaaacgg tcgggtgctt ctattacttc ttgaagacga actcgtagtg
     2341 cgcctcgacc tcgggctgcc ccagccagtt gcacgcgacc atctggacct tctgcttagc
     2401 ctttaggggc accgtgaccg actcctcggg tggtgcacca tcgggaacct gcgtgttctc
     2461 acccctggcg taggggatgt cgttcacgaa cacgtagatg tcgtgatccc ccgtgaatcc
     2521 ctctgagtgc atgctgagag tgcccgccgc aggagccgag aactcttggc cgacccagtg
     2581 aacgttctct tggccggcgg cgcagcccgg tcgagccgtc gacgtgtgac tcgagtagtt
     2641 ggggaacggc gccagtgaag ctccgaacga accggacacc ttcttcggtc ccgccggagc
     2701 tgccgtaggg atcgcaacca acaaggcgac gacgagtgct gcgagatatc tgcgcatgcc
     2761 cagagggttc gcccccgatc ggcacggtcc tgccactcag gcgcggggat cccacctcgt
     2821 cttgggagcg atcggctcca gcaggatgcg g
//
LOCUS       DATMED010000140         2334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3181781, whole genome shotgun sequence.
ACCESSION   DATMED010000140 DATMED010000000
VERSION     DATMED010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3181781"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VNP73_03490"
     CDS             <1..963
                     /locus_tag="VNP73_03490"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWL65016.1"
                     /translation="EQVRFVSSGTEAGMTAIRLARGVTGRKKIVKFAGCYHGHSDALL
                     VDAGSGIATLGIPGSPGVPEGTVADTIVATYNDPASVGQAVDAAAGDLAAIVVEPVAA
                     NMGLVLPSEGFLEGLREVADDAGCLLVFDEVITGFRLGPGGAQERFGVRADLVMLGKI
                     LGSGVPVAAVAGRSDVMSALAPEGDVYQAGTLSGNPLAMAAGAAALGLIKSDPTLYDR
                     IQETARNLIRGLVDAADEAKVPLFGAAIGGLAGFFFSDDEVVDYKGATRTHPDVYANF
                     FTGMLERGIYLPPSRFEALFLATVHTDGHIEKTVEAAADVFAEL"
     gene            944..1153
                     /locus_tag="VNP73_03495"
     CDS             944..1153
                     /locus_tag="VNP73_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65017.1"
                     /translation="MSSPSSDPPQTNWPLWRKLMLAGGFVLFGNALAEALMEPDPPRI
                     VGTAALVIGFVLLASGFGQRFKKKN"
     gene            complement(1163..1348)
                     /locus_tag="VNP73_03500"
     CDS             complement(1163..1348)
                     /locus_tag="VNP73_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65018.1"
                     /translation="MPQRLRAILAFASLLALTACTGGINAGGEQPTNNGGIAFIALAG
                     MLILAGIILWIILGREE"
     gene            complement(1348..2010)
                     /locus_tag="VNP73_03505"
     CDS             complement(1348..2010)
                     /locus_tag="VNP73_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65019.1"
                     /translation="MSIEFFTGMAAFISSIVIFCGSAFLLLMLIVGARLAYYITASIT
                     FGFVFIMGFVWSINPLGPVGQLPEWDPEDIAVEASELEAPGASEYPEGPWRAPNEDDD
                     VELAKAAELEGDASDFFATAVEEGDLEFDPTDSIVVVDDTTFLYEQGDGEYGATSFEV
                     LNSADKKVGEVVAILKYDPGNPLGKARAITAGTLVIFVLHLFGLSRAERKVKEEQPEG
                     LM"
     gene            complement(2011..>2334)
                     /locus_tag="VNP73_03510"
     CDS             complement(2011..>2334)
                     /locus_tag="VNP73_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWL65020.1"
                     /translation="EARCEVCHGPEGQGKEDPTLFDDGATLDEDLADPTDDDYPKWYP
                     GAALWKGDVRHLSKELHYLTIYNGRRFAFMPPFGEAPTQGIPVPPNPLTDKQIEAVMK
                     YERNL"
BASE COUNT          388 a          735 c          774 g          437 t
ORIGIN      
        1 gagcaggtgc ggttcgtgtc gtcggggacc gaggccggga tgacggcgat ccgtctcgcg
       61 cgcggggtca ccggccggaa gaagatcgtc aagttcgccg gttgctatca cggccactcc
      121 gacgccctgc tggtggatgc aggctccggg atcgcgaccc tcggcatacc cggcagtccg
      181 ggtgtaccgg aggggacggt cgccgacacc atcgttgcca cctacaacga cccggcatcg
      241 gtcgggcagg ccgtcgacgc ggccgcgggt gacctcgcgg cgatcgtcgt cgagccggta
      301 gccgccaaca tggggctcgt gcttccctcc gagggattcc tcgaaggttt gcgggaggtc
      361 gccgacgatg ccggatgcct ccttgtgttc gacgaggtga tcacggggtt ccgcctcggc
      421 ccgggcgggg cgcaggagcg gtttggagtc agagccgatc tggtcatgct gggcaagatc
      481 ctgggtagcg gcgtaccggt cgcggcggtt gccggacgaa gcgacgtgat gagcgcgctg
      541 gcccccgaag gggacgtcta ccaagcgggg acactctcgg gtaaccctct tgcgatggcc
      601 gccggtgcag cggctctcgg tttgatcaag tcggatccga cgctctacga ccggatccag
      661 gagaccgcac gcaacctgat ccgaggtctg gtcgacgcag ccgacgaagc caaggttccg
      721 ttgttcggag ctgcgatcgg tgggctggcc gggttcttct tctccgacga tgaggtcgtc
      781 gactacaagg gggcgaccag gacccatccg gacgtctacg cgaatttctt caccgggatg
      841 ctcgagcgcg gcatctatct accgccctcc cggttcgaag cgttgttcct cgcaacggtt
      901 cacacagatg gacatatcga gaagacggtc gaggcggctg cggatgtctt cgccgagctc
      961 tgatccgccg cagacgaact ggccgctgtg gcgcaagctg atgctggccg gaggcttcgt
     1021 cctcttcggt aatgcccttg cggaagcctt gatggaaccg gacccaccgc gcatcgtcgg
     1081 gacggccgcg ctggtgatcg gtttcgtgct attggcgtcg gggttcggtc agaggttcaa
     1141 gaagaagaac tgagtgtctg gactattcct cgcggccgag gatgatccac aggatgatgc
     1201 ccgcgaggat cagcatcccc gccagtgcga tgaaggcgat cccgccgttg ttggtcggtt
     1261 gctcgccccc ggcgttgatc ccgccggtgc acgccgtcag cgcgagcagg gatgcgaaag
     1321 cgaggatcgc gcgcagtctc tgaggcatca catcaaccct tccggctgct cttccttgac
     1381 cttacgctcg gcccggctga gaccgaacag atgcagaacg aagatcacga gtgtgcccgc
     1441 ggtgatcgcc cgggccttcc ccagcgggtt gcccgggtcg tatttcaaga tcgcaacgac
     1501 ctcgccgacc ttcttgtcgg ccgagttgag gacctcgaac gacgttgctc cgtactcacc
     1561 gtcaccctgc tcgtagagga acgtggtgtc gtccacgacc acgatcgagt cggtgggatc
     1621 gaactcgagg tcaccctcct cgacggccgt tgcgaagaag tcgctggcgt caccctcgag
     1681 ctcggctgcc ttggccagct cgacatcgtc gtcctcgttc ggggcccgcc acggtccctc
     1741 ggggtactcg gacgcaccgg gcgcctcgag ttcgctcgcc tcgacggcga tgtcctccgg
     1801 gtcccattcc ggcagctggc cgaccgggcc gagcgggttg atagaccaca cgaaccccat
     1861 gatgaagacg aatccgaacg tgatcgaagc ggtgatgtag tacgcgagtc gagctccgac
     1921 gatcagcatc aagagcagga acgcgcttcc gcagaagatc acgatcgacg agatgaacgc
     1981 cgccatcccc gtgaagaatt cgatgctcat ctagaggttc cgctcgtact tcatgacggc
     2041 ttcgatctgc ttgtcggtca gtgggttcgg aggcacgggg atgccttgcg tgggggcttc
     2101 accgaacggc ggcatgaacg caaaccttcg tccgttgtag atcgtcaggt agtggagctc
     2161 cttactgagg tgccggacgt cgcccttcca cagcgcggcg ccggggtacc acttggggta
     2221 gtcgtcgtcc gtcggatcgg caaggtcctc gtccagcgtc gccccgtcgt caaagagcgt
     2281 cggatcttct ttgccctggc cctcggggcc gtggcacacc tcgcagcgcg cctc
//
LOCUS       DATMED010000141         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1256474, whole genome shotgun sequence.
ACCESSION   DATMED010000141 DATMED010000000
VERSION     DATMED010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256474"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VNP73_03515"
     CDS             <1..564
                     /locus_tag="VNP73_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65021.1"
                     /translation="TPAPVLEFVSHLTWKTVSIPQTAIPITIKGHPYVVEVDEFGGEY
                     AVGAGRVIDIANEKKPFVTSNLRLEVNNPDAQLGEQADDPGASSALQGYDAHYCSVPK
                     REDPGIVACSFIVSGLRVFDITDPYHPKEIAYFNKAPEPNAAGPRGSYAMSGPAFAEK
                     RREIWYSDGYSGFYNVKITNGVWPSKN"
     gene            576..2744
                     /locus_tag="VNP73_03520"
     CDS             576..2744
                     /locus_tag="VNP73_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HWL65022.1"
                     /translation="MTRRFAVGLAAALVLTVLVPMSASATPVCTDGYMGGPPLAACGG
                     RIFPEAAVARSYIQYNPDPATGFREYQHGIEYLAEKYPRWISVFSFREHFKTPLAVSA
                     GEDGIRSYEKGDTKDGHDIWVVKITDHQVPDKEKLSLFFSLSVHGNERGGLEGGMRTA
                     EDLAIAADGEPDTAEELVDGIDNYETTTGRKPKFHTYDVREVLRKEVVYLVSFNIDGW
                     QVGDHNHQPPLPYTRGNSLGTDLNRQMPTVGRINESRNPLQESEMKYGHKLMHEVAKV
                     SGGRMAHAADLHGELTSNAYVDIMYPAGQFDSVDHRRLMAIAERTKSVIDETLYQGIV
                     NEAEEGSGGNSSSPDSDVPTKPAHWATVWDTLGYTDTGFIGDYMATDLAVTGMDYEFF
                     LNHTVPEKTWNVYMQENHINGARALIKVAMAYALYQDKEFNKRNLDVETNGRAGYVFN
                     PDTVTDRDKNGAGVLPGPAQDGIGADGKPIKQRHYSVTNMKWFKDTSRVMPTPFVKVL
                     SADVARSGHLDRLDTLVLADTVLPKDAKGRRVKSRAYFSNIKSWVKRGGNLVLTDRAV
                     HALAKLGVVDKGATTDIKVYLPYADIQDLEHPMVAGLRPNARQLVEAAILGYCIGNTC
                     SPMTIVDEAAFTEAGGHVVGTTEEGQVSVGQLKLGKGQIRVIGGALPTPTEEFDHRYG
                     LRDYAMTYSGLYIMENSIRHDAPGLGARPASALPAFLRVG"
     gene            2741..2989
                     /locus_tag="VNP73_03525"
     CDS             2741..2989
                     /locus_tag="VNP73_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65023.1"
                     /translation="MTRTARALSTSVALCLIATFVVVGSNHSALAQQALPCDNGSNGD
                     YSGDYAKLSEKGPYEIGKQEVVNLDSDLDGATIQRNLR"
     gene            3025..>3249
                     /locus_tag="VNP73_03530"
     CDS             3025..>3249
                     /locus_tag="VNP73_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL65024.1"
                     /translation="MIVQASSFFHPLQTMDLRACEPFLTENFIPHGYAVAFLAIRGTA
                     DSGGCMNLMGSGERADIDQAITWLGDQGWSN"
BASE COUNT          699 a         1015 c         1001 g          534 t
ORIGIN      
        1 actccggcac ccgtgctcga gttcgtcagc cacctgacgt ggaaaacggt gagcatcccg
       61 cagaccgcga tccccatcac gatcaagggt catccgtatg tagtcgaggt cgacgagttc
      121 ggcggcgaat acgcggtggg cgcaggccgc gtcatcgata tcgcgaacga gaagaagccg
      181 ttcgtcactt cgaaccttcg gctcgaggtg aacaatccgg atgcgcagtt gggagaacaa
      241 gccgacgacc ccggtgcatc cagcgctcta caggggtacg acgcccacta ctgctcggtg
      301 ccaaagcgtg aggatccggg catcgtcgcc tgcagcttca tcgtctcggg cttgcgagtc
      361 tttgacataa cggatccgta tcacccgaaa gagatcgcgt acttcaacaa ggcgcccgaa
      421 ccgaacgcgg cgggacccag aggaagctac gcgatgtccg gtcccgcttt cgccgagaaa
      481 cgaagagaga tctggtactc ggacggctac agcgggttct acaacgtgaa gatcaccaac
      541 ggagtatggc catccaagaa ctagggagga atctaatgac gcgtcggttc gcagtgggcc
      601 ttgccgcggc gctggtccta accgtcctgg tccccatgtc cgcctcggcg acaccggtgt
      661 gcacggacgg atacatggga gggccgccgt tggccgcgtg cggcggccgc atattccccg
      721 aggccgcggt cgcccgctcc tacatccagt acaaccccga ccctgcgacc ggattccgtg
      781 agtaccagca tgggatcgag tacctcgccg agaagtaccc ccgctggatc tccgtgttca
      841 gtttccgtga gcacttcaag actccgctcg ccgtgagcgc cggagaggac ggcatccgct
      901 cctacgagaa aggggacacc aaggacggcc acgacatctg ggtcgtcaag atcaccgatc
      961 accaggttcc cgacaaggag aagctctcgc tgttcttctc gttatcggtt cacgggaacg
     1021 aaagaggagg tctcgagggt gggatgcgga ccgccgagga cctcgcgatc gcggccgacg
     1081 gcgagcccga caccgccgag gagctggtcg acggtatcga caactacgag accaccaccg
     1141 ggcggaaacc gaagttccac acctacgacg tacgagaggt gctccggaaa gaggtcgtgt
     1201 acctcgtcag cttcaacatc gatgggtggc aggtgggcga ccacaaccat cagccgcctc
     1261 tcccctacac acgcggaaac agcctgggaa ccgatctcaa ccggcagatg ccgaccgtcg
     1321 gtcggatcaa cgagtcacgg aacccgctgc aagagagcga gatgaagtac ggccacaagc
     1381 tcatgcatga agtggcgaag gtgtccggag gacgcatggc tcacgccgcc gacctccacg
     1441 gggagctcac ttcgaacgct tacgtggaca tcatgtatcc ggccggtcag ttcgactcgg
     1501 tcgaccatcg tcgactgatg gcgatcgccg agcggaccaa gtccgtgatc gacgagacct
     1561 tgtaccaagg catcgtcaac gaagccgagg agggatcggg tggcaacagc agctcacccg
     1621 acagtgacgt acccacgaag cccgcgcatt gggccacggt gtgggacacg ctcggctaca
     1681 ccgacaccgg cttcattggg gactacatgg ccaccgacct ggccgtcaca gggatggatt
     1741 acgaattctt cctcaatcac acggtgccgg agaagacctg gaacgtttac atgcaggaga
     1801 accacatcaa cggggcccgc gccttgatca aggtcgcgat ggcttacgcg ctgtaccagg
     1861 acaaggagtt caacaagcgc aacctcgacg tcgagacgaa cggccgtgcg ggatacgtct
     1921 tcaaccccga caccgtgacc gaccgtgaca agaacggagc cggcgttctt cccggacccg
     1981 cgcaggatgg gatcggcgcc gacggcaagc ccatcaagca gaggcattac tcggtcacga
     2041 acatgaagtg gtttaaggac acgagccggg tgatgccgac accgttcgtg aaggtgctgt
     2101 cggccgatgt tgcgagatcc ggccacctcg atcgtctcga caccctggtg ctggccgaca
     2161 cggtgctccc gaaggacgcc aaaggaaggc gcgtgaagtc cagggcgtac ttctctaaca
     2221 tcaagtcgtg ggtgaagcgc ggaggcaacc tggtgctcac cgaccgcgca gtgcacgctc
     2281 tcgccaaact cggcgtggtc gacaagggcg caaccaccga catcaaggtc tatctgccgt
     2341 atgccgacat ccaggacctc gaacacccga tggtcgcagg cttgaggccc aacgcgcgac
     2401 agcttgtgga ggcagcgatc ctcgggtact gcatcggcaa cacctgctcg ccgatgacga
     2461 tcgtcgatga agcggcgttc accgaagccg gtgggcacgt ggtcgggacc accgaggagg
     2521 gtcaagtgag cgtgggccaa ctgaaactcg gcaaaggtca gatccgcgtg atcggaggcg
     2581 ccttgccgac cccgaccgag gagtttgacc accgctacgg tctgcgcgat tacgcgatga
     2641 cctactccgg gttgtacatc atggagaaca gcatccgcca cgacgccccg ggactgggag
     2701 cgcgccctgc aagcgcactt ccggcattcc tgagggtggg atgactcgaa cggccagggc
     2761 gctctcgaca tccgtcgctc tctgcctgat cgcgacgttc gtcgttgttg gttcgaacca
     2821 ttctgcccta gcgcagcagg cgcttccgtg cgacaacggc tcgaacggcg actactcggg
     2881 tgactacgcc aagttgtccg agaaggggcc ctacgagatc ggcaaacaag aggtcgtaaa
     2941 cctcgacagc gacctcgacg gcgcaacgat ccaacgcaac ttgcgctgat ccgccccaag
     3001 gttccggccg gaaccgaagt gccggtgatc gtccaggcct cgtcgttttt ccacccactt
     3061 cagacgatgg atctgcgcgc ttgcgagccc ttcctgacgg agaacttcat cccgcatgga
     3121 tatgccgtgg ctttccttgc gatcagaggc acggcggact cgggcggctg catgaatctg
     3181 atgggctccg gtgagcgcgc cgacatcgat caggcgatca cgtggctcgg cgaccaggga
     3241 tggagcaat
//
LOCUS       DATMED010000142        10731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5101178, whole genome shotgun sequence.
ACCESSION   DATMED010000142 DATMED010000000
VERSION     DATMED010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5101178"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VNP73_03535"
     CDS             <1..1233
                     /locus_tag="VNP73_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWL65025.1"
                     /translation="TGRPFDGRVRRIFEVFLERNIPARHEAQLTYVDGQPYLRSRSVV
                     PYRLDQDETLTQRWANIEESDRGQVDTPGGPIEYLAVPVRSEGETAGVFVVAVFRELW
                     LDEIDPAIVGAAGVGAIVVLIGSFLAWRLADRILGPVEKVTEAARGISESDLRGRIEI
                     TGADEIARLAQTFNEMLDRLERAFSTQRRFIDDASHELRTPITVIQGQLDVLGDDPED
                     RRKTLEIVMDELERMSRFVTDLLLLARADQPDFLALGSVDVSTLSEEVFTKAQSLAPR
                     DWRMDDIGRGLMIGDRQRLTQALIQLAQNAVQHTHEGDVISMGSSVSDGHVRFWVSDT
                     GPGIPPSEQSHIFDRFHRGHPRTASSEGAGLGLSIVRAIAEAHHGRVDLNSRLGEGSS
                     FSIVLPVDQPEPSERVTV"
     gene            1230..1895
                     /locus_tag="VNP73_03540"
     CDS             1230..1895
                     /locus_tag="VNP73_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65026.1"
                     /translation="MKRILIVEDEARISSFLEKGLKSNGFTTSVAATGSDGLALAATG
                     EFDLVILDIGLPDTDGFSVLADLRSHDRDIPVVILTARDAVADTVHGFEGGADDYVTK
                     PFRFEELLARIKARLREDRRPESFVLEAGDAKLDLRTRRISRGDSTIELSAREFALAE
                     LFFQHPGQVLTREQLLSNVWGYDYDPGSNIVDVYVGYLRKKLGGGTIKTVRGMGYRLE
                     TGE"
     gene            complement(1922..2482)
                     /locus_tag="VNP73_03545"
     CDS             complement(1922..2482)
                     /locus_tag="VNP73_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010998504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65027.1"
                     /translation="MKFLRMAAATAMVASMMVVGGGVANAEERRCIGTIGAKTLDNVK
                     VPAGETCTLKGTTVKGTITVNRNARLYAIGARVVGNIQGENARLVEVRRGRVGGSIQV
                     VQGGRGFIKNVRVNADILFDDQRRRVEVRSNTVGGNIQLFQNTGDVLVRNNVVDGNLQ
                     CKENSPAPTGGGNKVQGSKEDQCSGL"
     gene            complement(2646..2718)
                     /locus_tag="VNP73_03550"
     tRNA            complement(2646..2718)
                     /locus_tag="VNP73_03550"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2683..2685),aa:Ala,seq:cgc)
     gene            complement(2755..3378)
                     /locus_tag="VNP73_03555"
     CDS             complement(2755..3378)
                     /locus_tag="VNP73_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HWL65028.1"
                     /translation="MNDLADRLRKEFDAKTEAREQGLARARDAIRSCGNAIRAMHRHE
                     TDVATGLLEDAQAQLDAARIALKDHPEVLFAGFVHDAEKEVAEARVTFALVTGAAFPT
                     LDDLGVTPAAFVKGMAEAIGEMRRHILDLMRHGDLKRCEELLGSMDDIYLVLVSMDYP
                     DGITHGLRRLTDVARSIIERTRGDFTTSSIQSSLKDALTKHAEDLDR"
     gene            complement(3408..3764)
                     /locus_tag="VNP73_03560"
     CDS             complement(3408..3764)
                     /locus_tag="VNP73_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65029.1"
                     /translation="MKLISPHRNVGRWVVVPPGGPGSATPVSKQQSQRRRKFILLALL
                     SFALATGIWSLVTRGDLLELHLVADAGCVFYASLLLDAKRRRVEHRHKVRKLPRPEDK
                     ASDYFEVIEAGGTRNA"
     gene            4026..5486
                     /locus_tag="VNP73_03565"
     CDS             4026..5486
                     /locus_tag="VNP73_03565"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWL65030.1"
                     /translation="MSIEVMPAKGDVTSIACDALIVAAGSKDGSVVLSEAAEHLDREF
                     DGYLSSYLKEISYKGEGREIAIFPSFGKVAASTIVVVGVDKKGDGIKRVRRAAAACAR
                     RLSERSVIASTLHSVEAGTVAASIEGFVLGAYKSPTRKKDAKKSKIERIELLDADKAD
                     IARGLVYAAATIIARDLVNQPGSELTPTTFAERAQLIASETGLDCDVLDEKELRKRGF
                     GGLLAVAQGSVNPPKLIQLRYSPRGAKERVALVGKGVTFDSGGLSLKTAGGMEDMKTD
                     MGGGAAVIGAMSALSRLKPKIEVIALIPATENMPSGSAIKPGDVIQHYGGLTTEVLNT
                     DAEGRLILADALVLASEQEPKAIIDAATLTGSMSIALGHKMTGAFSNDDELWEEFAAA
                     AGRAGEPMWRMPLVEDYRKSLDSDVADMKNIGTRYGGAVVAALFLREFVGEKIPWIHL
                     DIAGTGRAEGESDDGPRGATGVATRTLIAWLEKRGR"
     gene            5483..5776
                     /locus_tag="VNP73_03570"
     CDS             5483..5776
                     /locus_tag="VNP73_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65031.1"
                     /translation="MTNGHEVVRLPREPEPVTAEPVTPPGRLHGLAIAGMAVSALIGI
                     AAMLAFITINWPGDSNRYLVFAFVAAGLGFLTCASAAVLTAARDTYAKHRPTE"
     gene            complement(5847..6290)
                     /locus_tag="VNP73_03575"
     CDS             complement(5847..6290)
                     /locus_tag="VNP73_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWL65032.1"
                     /translation="MSSLHCNGVEIAERVRTARSAWSRLRGLFAQPIAEGEALVLEPA
                     RQIHTVGMRVPIDVVFCDREWVIVHLIREMRPGRVSRWVRNAYRAIELPAGAISDRIG
                     LGDLVQPGLVCLGGEKDPCARNLAGLLGDEAKSVDARPRAGPISS"
     gene            6358..6510
                     /locus_tag="VNP73_03580"
     CDS             6358..6510
                     /locus_tag="VNP73_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HWL65033.1"
                     /translation="MQKWEYATVPLISHALQEILNQWGEEGWELVQVVESQATGTTAF
                     LKRPKP"
     gene            complement(6516..7403)
                     /locus_tag="VNP73_03585"
     CDS             complement(6516..7403)
                     /locus_tag="VNP73_03585"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HWL65034.1"
                     /translation="MSVTKAVIPAAGFGTRFLPATKAIAKELITVVDKPGIQYAVEEA
                     ARAGIKDILVVTSRGKGAIEDHFDRHGELEDLLEAAGKTEELKSVREVGSHAKVHFVR
                     QKAATGLGHAVLEAREHVGDEPFVVLLPDEIVPEPVDDEVPLLPRLIELHQETGSCVI
                     AVRRVPREDVVKYGVIEHGEMTGDHTQVKSLVEKPPVDEAPSDLSLPGRYLFTPTIFD
                     AIERTKAGVGGEYQVTDAIDLLARQGDVLAYVHEGPIYDYGKKFEFLKTTIMLALRRN
                     DLGKPLREFLIQLQDELNA"
     gene            complement(7413..9413)
                     /locus_tag="VNP73_03590"
     CDS             complement(7413..9413)
                     /locus_tag="VNP73_03590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWL65035.1"
                     /translation="MSLQRKLTLFFVTIVMLPLVAAAFIVQRVISDEVGERAVLTMRP
                     TLDATMALYNDDLAQLEQRVRSSISQPGKLSAALESSDRAELKKYLQARLGASSNLDF
                     LLIQRDRSILAYVANEPRFLSGVPVPNAFEVAAAKEGSGKGFVRSRAVPLEVAGAGPI
                     AQISGGMWVDRELLAAGWRSIVGLSVVADGDVLASTASVPGPVRINVARTGPFEASIG
                     GPVIAVARPLEGEMSIVASTPSGPIDSASGRVITAMLILLLFALTVTTVLAYVLARAI
                     TQPLDELAEGAMAISEGRFDHQIPVRSKDEVGQLAQAFNKMTSHLSTTISELSWSRDQ
                     LRKSIQRVGDTLRSTHDMKQLLESVLATAADAVQAEAAVFWAFTPTREELHPSISRGT
                     EIDPLVRVGVGEGAVGLVAERATPVLIPNQAAGGPRFSRYEPRFPVAVAVPVYSQDKI
                     RGVLALYREDPRKHFTEQDVEMVTFLAEQGGTAIENVLLHEEAQRLSITDGLTGIWNR
                     RYFQMQFRQVLATATRFDRTFSILLLDLDHFKRVNDTYGHRRGDEILVEFSQRVTTMV
                     REVDTFARYGGEEFACLLTETEMSGALTTAEKIREAVRREAFGEGEEPQLHLTVSIGV
                     ATYPDHGDSFVSLIEAADKALYRAKQTGRDQVCVAERLRLAT"
     gene            9530..9832
                     /locus_tag="VNP73_03595"
     CDS             9530..9832
                     /locus_tag="VNP73_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWL65036.1"
                     /translation="MPVYEYACTACGERTEARQSFDDPPLEECPVCGGKLRKLYSPVG
                     IVFKGSGFYSTDAKKKGDSATKTSKSDTSKKSGEKKSEPASSSSTSSSSGKDSTSK"
     gene            complement(9876..10538)
                     /locus_tag="VNP73_03600"
     CDS             complement(9876..10538)
                     /locus_tag="VNP73_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65037.1"
                     /translation="MSRFIKLIAVTGAMLLVAGIAWAQTFDVTVSFELTDTKALAQTG
                     INIHIEQPAGQPEMGHVTLTIPRGFKVPGDAKMADGDQVGTADLSIDVGPACSGAGPG
                     TAPAQFPDRVIVERDRTDEQADRGVKAVWVVDLQPVTTIPLEITGSKKKGWKLDGDIP
                     ANDHTCPPIVFDAQIGPTSAAGVPVLTNAATPKDYVFKAIFTSQQSPDVDTNLQTITI
                     TP"
BASE COUNT         2116 a         3294 c         3256 g         2065 t
ORIGIN      
        1 acgggccggc cgttcgatgg gcgtgtacgg cgcatcttcg aggttttcct cgaacgcaac
       61 atcccagccc gccacgaagc acagctgacc tacgtcgatg gacagccata tctccgaagt
      121 cggtcggtcg tcccctaccg cctagaccag gacgagacgc tcacccaaag gtgggcaaac
      181 atcgaggagt ccgatcgcgg gcaggtcgac acgcctggag gcccaatcga gtacctagcc
      241 gttcccgtgc gttctgaagg cgagaccgcc ggggtattcg tggttgcggt cttccgagag
      301 ctctggctcg atgagatcga tcctgcgatc gtcggggcgg cgggagttgg agcgatcgtg
      361 gtgttgatcg gctctttcct cgcctggcgg ctggccgatc gcatccttgg ccccgtcgag
      421 aaggtgaccg aagccgcgcg cggtatatcg gaatccgatc tgcgcggtcg catcgagatc
      481 actggggccg acgagatagc gcggcttgcg cagaccttca acgagatgct cgatcgattg
      541 gaaagggcgt tctcgactca gcgtcgtttc atcgacgatg ccagccacga gctcaggacc
      601 ccgatcacgg tgatccaagg acaactcgat gtgctcggag atgatccgga ggatcgccgg
      661 aagaccttgg agatcgtgat ggacgagctc gaacggatga gtcgattcgt tacggacctc
      721 ctgcttcttg cacgtgcaga tcaacccgac ttcctagccc tcggcagcgt cgacgtatcc
      781 acactgtcgg aagaggtgtt cacgaaggcc caatctctgg ccccccgcga ctggcggatg
      841 gatgacatcg gtcgcggatt gatgatcggt gatcggcagc gtttgaccca ggcgctgatc
      901 cagctggctc agaatgccgt tcagcacact catgaaggcg atgtgatctc gatgggctca
      961 tcggtttcgg acgggcacgt ccgcttctgg gtcagtgaca ccgggccggg catcccaccc
     1021 agtgagcaat ctcatatctt cgaccgattc caccgcgggc acccacggac agcgtcttcg
     1081 gagggagcgg gtcttggatt gtccatcgtc agggctattg ccgaagcaca ccatgggcgg
     1141 gtcgatctga acagccgact aggagaagga tcgagtttct cgatcgtcct tccggtggat
     1201 cagcccgagc cgagtgagcg ggtgacagtg tgaagcgcat cctcatcgtc gaggacgagg
     1261 ccaggatctc ttcattcctc gagaaaggcc tgaagtcgaa cgggttcacg acctcggttg
     1321 cagcaactgg gtcggatggc ctggctctcg ctgcaacggg ggagttcgac cttgtcatct
     1381 tggatatcgg cttgccagac actgatgggt tcagcgttct tgccgacctc cgtagtcatg
     1441 accgggatat ccccgtggtc atcctgaccg cccgggacgc ggtggccgat accgtgcacg
     1501 gcttcgaagg cggggccgac gattacgtga cgaaaccatt ccgcttcgag gagctgcttg
     1561 cccgtatcaa ggcacggctg cgtgaggacc gtcgtcctga atctttcgtg ctggaggccg
     1621 gcgatgcaaa gctcgatcta aggacccgca ggatctccag gggagattcg acgattgagc
     1681 tcagcgcccg cgagttcgca ctagcggagc tctttttcca gcatccggga caggtgctca
     1741 cccgcgagca gctcctgagc aatgtctggg gctatgacta tgaccccgga tcgaacatcg
     1801 tagacgtcta cgttggctac ctacggaaga agcttggagg cggaacgatc aagaccgtgc
     1861 gcgggatggg gtatcggctc gaaacaggcg aatgacgttt cggcttcgag ccgatccttg
     1921 cctagagccc gctgcactga tcttctttgc tcccctgcac cttgttgcct cctccggtgg
     1981 gggcgggaga gttctctttg cattgaaggt tgccatctac gacgttgttc cttacgagaa
     2041 cgtcgcctgt gttctggaag agctggatgt tgccgccgac ggtattgctc cgaacttcga
     2101 cccggcgcct ctgatcgtcg aaaaggatgt ccgcgttaac gcggacgttc ttgatgaagc
     2161 cgcggccacc ttggacgacc tggatactcc caccgacccg gccacgtcgc acctcgacga
     2221 ggcgagcgtt ctcaccttgg atgttgccga cgaccctagc tccgatggcg tagagcctcg
     2281 cattgcggtt caccgtgatc gttcctttga cagtggtgcc cttgagagtg cacgtctcgc
     2341 cggcaggtac cttcacgttg tcgagcgtct tcgccccgat cgtgccgatg caccgccgtt
     2401 cttctgcgtt cgccacgccc cctccaacca ccatcatcga ggccaccatt gcggttgctg
     2461 cagccatgcg gagaaacttc atcatctact ccctctcatc tcgtctgatg gactcatgct
     2521 gcccagcagg agtaagagga gaatgagggc ctcatgagac tttccttacc catgagctct
     2581 gaacggaggc ttcgcccaag aacatctcct ctagagagtc agcggcgaac gtagcggctg
     2641 tttcgtggac cagaggagat tcgaactcct gacctcaccc gtgcgagggg tgcgctctac
     2701 caactgagct actggcccgt aagagcgaat cctacgggag ccgtcgaagg cgtcctagcg
     2761 gtcgagatcc tcggcgtgtt tggtcagtgc atccttcagc gagctctgga tgctcgaggt
     2821 ggtgaaatcc ccccgggtgc gttcgatgat cgagcgcgcg acatcggtca atcgacgcag
     2881 cccatgagtg atgccgtccg ggtagtccat cgagaccagc acgagataga tgtcgtccat
     2941 cgaaccgagc aactcctcac accgcttcag atcgccatgt cgcatcaggt cgaggatgtg
     3001 tcgcctcatc tccccgatcg cttccgccat gcccttcacg aaagcggccg gcgtcactcc
     3061 caagtcgtcg agcgtcggga atgcggcccc cgtcaccaga gcgaaagtga cccgcgcctc
     3121 ggcgacctct ttctcggcat cgtgaacgaa gccggcgaac aagacctcgg gatggtcctt
     3181 cagcgcgatg cgcgccgcgt ccagttgagc ttgcgcgtct tcaagcagcc ctgtcgcgac
     3241 gtccgtctcg tgcctgtgca tggccctgat cgcgttgccg cacgatcgga tcgcatcgcg
     3301 ggcgcgagca agtccttgct cccgggcttc ggtcttggcg tcgaactcct tacgaagtcg
     3361 atcagcgaga tcgttcatgt ccacacgtgc accctagttg acgcagatca ggcgttacgg
     3421 gtgccgccgg cctcgatgac ctcaaagtag tcggaagctt tgtcctcggg acgaggcagc
     3481 ttgcgaacct tgtggcgatg ctcgacgcgc cgcctcttcg catccaacaa cagcgacgcg
     3541 tagaaaacac aaccggcatc ggcaacgaga tgcagttcga gtagatcgcc tcgagtaaca
     3601 agtgaccaga tcccggttgc caaggcgaac gaaagcagtg ccaacaggat gaatttgcgt
     3661 cggcgttgac tctgttgttt cgagacagga gttgcgcttc ccggcccccc cggtggaacg
     3721 acgacccatc gacccacatt gcgatgtgga gagatcagtt tcatgcgtcg tttgaaccgc
     3781 ataacggcgg ggagcggaga actttgtcta gcccgccgag tggcgggtag ataaacgacc
     3841 agccatgcga tggccaagaa ggccaaaaac cacagtgtca agagctcttc cccaagctct
     3901 agtgctacaa gaacccaacc gtacccctgt cgagtgcgcg gctcaaggat ttgaacgcga
     3961 attcgcgacc tcccaaaagc tatggtccgt caatgaacga acacccggac aactaaggag
     4021 cgaatgtgtc gatcgaggtg atgcccgcaa aaggtgacgt cacgtccatc gcatgtgacg
     4081 cgttgatagt tgcggccggc tccaaggatg gctcggttgt gttgtccgag gccgccgaac
     4141 acttagaccg cgaattcgac ggctatctct cttcgtacct gaaggagatc tcttacaagg
     4201 gagaagggcg cgagatcgcg atctttccgt cgttcgggaa ggttgccgcc tccacgatcg
     4261 tggtggtggg cgtggacaag aagggtgacg ggatcaagcg agtaaggcgc gcggcggcag
     4321 cgtgcgctcg ccgcctctcg gagcgatcgg tcatcgcctc gaccctgcac tcggtcgagg
     4381 ccggcaccgt ggcagcttcg atcgaagggt tcgtcctggg ggcctacaag agccccaccc
     4441 gcaagaagga cgcgaaaaag tccaagatcg agcggatcga gctgctggac gccgacaagg
     4501 cagacatagc gcgcggcctc gtgtacgcgg ccgcgacgat catcgcccgc gatctggtca
     4561 accagcccgg ttcagagctg accccgacca ccttcgcgga acgagcccaa ctcatcgcct
     4621 cggagaccgg cctggactgc gatgtgctcg acgagaagga gctgcgcaag cgtggattcg
     4681 ggggtctatt ggccgtcgcg caaggatcgg tcaacccacc gaagctcatc cagctccggt
     4741 attcaccgag gggggccaag gagagggtgg cactggtcgg gaagggcgtc acgttcgact
     4801 ccggcgggct ctcgctgaaa accgccggtg ggatggaaga catgaagaca gacatgggcg
     4861 gcggtgcggc cgtgatcggt gccatgtcgg ccttgtcccg gctgaagccc aagatcgagg
     4921 tcatcgccct catccccgcc accgagaaca tgccatcggg cagcgccatc aagcccgggg
     4981 acgttatcca gcattacgga ggcctaacga ccgaggtcct taataccgac gcggaagggc
     5041 ggctgatcct cgccgacgcc ctggttctgg cctcggaaca ggaaccgaaa gccatcatcg
     5101 acgcggccac tcttaccgga tccatgtcga tcgccttggg acacaagatg accggggcct
     5161 tctctaacga cgacgagcta tgggaagaat tcgcggccgc ggccggcagg gcgggcgagc
     5221 ccatgtggcg catgcccctc gtcgaggatt accggaagtc gcttgattca gacgtagcgg
     5281 atatgaagaa catcggaacg aggtatggag gggcagtggt ggcggccctc ttcttgcggg
     5341 agttcgtcgg cgagaagatc ccctggatac acctcgacat cgctggaaca gggcgggcgg
     5401 aaggagaaag cgatgacgga ccgcgcggag caaccggggt cgcgacgaga acactcatcg
     5461 cgtggctaga gaagagggga cgatgaccaa cggacacgag gttgtaaggc ttccgcgcga
     5521 acccgagccg gtcaccgcgg aaccggtaac accgccggga cgcttgcacg gacttgccat
     5581 cgcaggcatg gcggtctcgg ctctcatcgg gatcgcggcc atgctcgctt tcatcacgat
     5641 caactggccg ggcgattcga atcgttacct tgtgttcgcc ttcgtagctg caggtctcgg
     5701 cttcctgacg tgcgcatcgg cagccgtgct gaccgcagcg cgcgacacat acgcgaagca
     5761 cagacccacc gagtaaggga tccactatgg agatcgaagg gcacccgact caggacatcg
     5821 tcgaggaact cgaacgccga ggggcactac gagctgatgg gtccagctcg gggccgcgcg
     5881 tcgacgctct tcgcttcatc accgagaagg ccggcgaggt tgcgggcaca tgggtctttc
     5941 tcccccccga ggcatacgag accgggttgg acgaggtccc ctaacccgat ccgatcggag
     6001 atcgcgccgg ccggaagttc gatcgcgcgg tacgcgtttc tcacccatcg actgacgcgg
     6061 cccggccgca tctctcggat cagatgcacg atcacccatt cacgatcgca gaagacgaca
     6121 tcgatgggca cacgcatccc aaccgtgtgg atctgacgag ccggttcaag gaccaaagct
     6181 tcgccctccg cgatcggctg cgcgaaaaga ccccgcagcc gagaccacgc agatcgagcc
     6241 gtacgaacac gttcggctat ctcaacgccg ttgcaatgaa ggctcgacat ctcggacacc
     6301 tacagtaggt tgctcgccgt tccaactaag cgactcagac cacaagtgag gtgcgctgtg
     6361 cagaaatggg agtacgccac ggttccgctt atctctcacg cgctacaaga gatcctgaat
     6421 caatggggcg aagagggctg ggagctggtt caggtcgtgg agagccaggc aaccggcacg
     6481 accgctttcc tcaagcggcc caagccatag gactgttacg cgttcagttc gtcctgtaac
     6541 tggatcaaga actcccgcaa gggcttgccc aggtcgttgc gcctgagcgc cagcatgatc
     6601 gtcgtcttga ggaactcgaa cttcttcccg tagtcgtaga taggtccctc gtgaacgtag
     6661 gcgaggacat caccttggcg ggccagcaga tcgatggcgt ccgtcacctg atattcgcca
     6721 ccgacacccg ccttcgtgcg ctcgatcgca tcgaagatcg tcggggtgaa caggtagcgg
     6781 cccggaaggc tcagatcgga cggcgcttcg tccaccggag gcttctcgac cagactcttc
     6841 acctgggtgt ggtcgccggt catctctccg tgctcgatga ctccgtattt gacgacgtct
     6901 tccctcggca cgcgacgaac cgcaatgacg caggatccgg tctcctggtg aagctcgatg
     6961 agccgtggaa gcaacggcac ctcatcgtcg acgggctcgg gaacgatctc atcgggcagc
     7021 aggaccacga acggctcgtc ccccacgtgc tccctcgcct cgaggaccgc atgccccaaa
     7081 ccggtcgcgg ccttctgtcg cacgaaatgg accttggcgt gggacccaac ttcccgaaca
     7141 cttttcagtt cctcggtctt accggcggcc tccagcagat cttcgagctc tccgtggcga
     7201 tcgaagtggt cttcgatcgc gcccttccct cggctcgtga ccacgaggat gtccttgatg
     7261 ccggcgcggg cagcttcctc cacggcgtat tggatgccgg gcttgtctac gacggtgatc
     7321 agttctttgg cgatcgcctt cgtcgccgga aggaatctcg taccgaaccc cgcggccggg
     7381 atgactgcct tggtgacgga catccgggaa ccttacgttg cgagacgaag cctttccgcg
     7441 acgcatacct ggtcgcggcc ggtttgcttg gctcgatata aggctttgtc tgctgcctcg
     7501 atcagcgaga cgaacgaatc accgtgatca gggtaagtcg caacaccgat cgacaccgtt
     7561 aggtgcagct gcggctcctc gccctcgccg aaagcctctc tccggacggc ttcccggatc
     7621 ttctccgcgg tcgtcagagc gccgctcatc tcggtttccg tcaggagaca cgcgaactct
     7681 tcgccaccat aacgagcgaa cgtgtcgact tcacgaacca tcgtcgtaac ccgctgcgag
     7741 aactcgacca ggatctcgtc accccggcgg tggccgtagg tatcattgac cctcttgaag
     7801 tggtcgaggt ccaacaagag gatgctgaac gtgcgatcga atcgggtcgc ggttgcgagt
     7861 acttgccgga actgcatctg gaagtacctg cggttccaga taccggtcag accgtcggtg
     7921 atcgaaagac gctgagcctc ttcgtgcaag agcacgttct ctatggcggt gccgccctgc
     7981 tccgcaagga aggtgaccat ctcgacgtcc tgctcggtga agtgcttgcg gggatcttcg
     8041 cgatacagag cgagaactcc tctgatcttg tcctggctgt acacgggcac cgcgacggca
     8101 acggggaatc gcggttcgta ccgagagaag cgcggccccc ccgcggcctg attggggatg
     8161 agaacaggag tcgcacgctc cgcaacaagc cccacggccc cttcaccgac cccgacccgt
     8221 acgagtggat cgatctcggt gccgcgactg atcgaggggt gaagttcttc ccgggtcggc
     8281 gtgaaggccc agaacaccgc ggcttcggcc tgcacggcgt cggccgccgt ggccagaacg
     8341 gactcgagca actgcttcat gtcgtgcgtc gacctcaacg tgtcccccac gcgctggatc
     8401 gacttgcgca gttggtcacg cgaccaggac agctcggaga tcgtcgtgga gagatggctt
     8461 gtcatcttgt tgaaggcttg agccagctgg ccgacctcgt ccttggagcg gaccgggatc
     8521 tgatgatcga aacggccttc agagatcgcc atcgctccct cggcaagttc gtcgagcggc
     8581 tgggtgatgg cccttgccaa gacatatgcg aggacggtcg tcacggtcaa cgcaaacaga
     8641 agcaatatca gcatcgccgt gatgacacgt ccggaggcac tgtcgatcgg accggacggc
     8701 gtcgacgcca cgatcgacat ctcgccctcg agaggacgcg ccaccgcgat cacaggacca
     8761 ccgatagatg cctcgaacgg accggtgcga gcaacgttga tcctcaccgg acccggaaca
     8821 ctggccgttg aggcgagcac gtcaccgtcc gcgacaaccg agagtccgac gatggatcgc
     8881 cagcccgcgg caagtaactc gcgatcgacc cacatgcccc cacttatctg tgcgatcggc
     8941 ccggcgccgg caacctcgag aggtaccgct cgtgacctaa cgaatccctt gcccgacccc
     9001 tctttcgcgg cagcgacttc gaaggcgttc gggaccggaa ccccggagag gaagcgcggc
     9061 tcgttcgcga cgtaagccag gatcgatcgg tcacgctgga tcagcaggaa gtcgagattc
     9121 gaagaagcac ccaaccgagc ctgtaggtac ttcttgagct cggcgcgatc ggaagattcc
     9181 agagccgcac tcaacttgcc gggctgactg atggaactcc tgacccgttg ctccagctga
     9241 gcgagatcgt cgttgtacaa ggccatggtt gcgtcgagcg tcggcctcat agtgaggacc
     9301 gccctctcgc ctacctcgtc ggagatgacc cgttggacga tgaaagcggc cgcgacgaga
     9361 ggcaacatca cgatcgtgac gaagaaaagt gtcagcttcc gctgtaggga catctagtta
     9421 tccctttttt ccgatatttc ggtccgcctg ccgtacaact ttaaggggcg caaggctcca
     9481 aggccagaca tcagggggtg ggcgggtgcc gcagtgagat gattccccca tgccggtata
     9541 cgaatacgcc tgtaccgcct gcggcgaaag gaccgaagcg cggcagtctt tcgacgatcc
     9601 tccgctcgag gaatgccccg tgtgtggggg gaaactcagg aagctgtatt caccggtggg
     9661 catcgtcttc aagggctcgg gcttctattc cacagacgcc aagaagaagg gcgattcagc
     9721 cacgaaaacg tcgaagtcgg acacttcgaa gaagtcgggc gagaagaaga gcgagcctgc
     9781 gtcatcatca tcgacatcat catcgtccgg taaggattcc acctccaaat gaaaagggcc
     9841 cccgtttccg ggggcccttt tttcactcaa acgggttatg gcgtgatcgt gatcgtctgt
     9901 aggttcgtgt ccacgtcggg agactgctgc gacgtgaaga tcgccttgaa cacgtagtcc
     9961 ttgggcgttg ccgcgttcgt caggaccgga acgccggcgg cggaagtcgg accgatctgt
    10021 gcatcgaaga cgatcggcgg gcacgtgtga tcgttagccg ggatgtcgcc gtcgagtttc
    10081 catcccttct tcttgcttcc ggtgatctcg agagggatcg tggtgaccgg ctgaaggtcg
    10141 acgacccaaa ccgccttgac gccgcggtcc gcctgctcat ccgtgcgatc tctttcgacg
    10201 atcacacggt cggggaactg tgcgggggcc gtgccgggac ctgctccgga acaagccggg
    10261 cccacgtcga tgcttaggtc tgccgttccg acctggtcgc cgtcggccat ctttgcgtca
    10321 cccggcacct tgaaacccct cgggatcgtg agcgtcacgt gacccatctc gggttgaccg
    10381 gccggttgct cgatgtggat gttgatccca gtttgcgcga gagccttcgt gtcggtcagt
    10441 tcgaaggaaa ccgtcacgtc gaacgtttgc gcccacgcga tcccagctac gagaagcatc
    10501 gctcccgtaa ccgctatcag cttgatgaac ctgctcatgt tgtcctccct cagcacccct
    10561 cttcagcttg ggcgcaaaga cattccttgt gagttgtcag attcgcccaa ataccttgat
    10621 ttcctgcgct catgtacgag ctacatatgc tgcggcacct atgtcagtcc tgcaagcact
    10681 cgttcttggc gcagttcaag gtttgaccga gttcgtcccg atctcgtcat c
//
LOCUS       DATMED010000143         7051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_875395, whole genome shotgun sequence.
ACCESSION   DATMED010000143 DATMED010000000
VERSION     DATMED010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875395"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VNP73_03605"
     CDS             <1..529
                     /locus_tag="VNP73_03605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65038.1"
                     /translation="CLLFFGRNDVNQKGLDLLIEGYALARARGLDLPLVIGGRAHEDS
                     ANYLTEAVKRQGVSEHVSFVGETSDQERSRLMSNARAFVFPSRWDGPPRPVREAIALG
                     VPVLVTPGTNMAEVVAEHDAGAVMQPTPEGVRDALIQLSDVDRVERWRRNSEKLRAAL
                     SWPKLARLYLAAYEG"
     gene            572..1447
                     /locus_tag="VNP73_03610"
     CDS             572..1447
                     /locus_tag="VNP73_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65039.1"
                     /translation="MRVSATASRTGSAATGKSTPPITRIRPIGGWFDLNLREFWAYRE
                     LIYFFVWRDLKVRYKQTFVGAVWVILQPTATMILFTVFFGGVLGLPTGGLPDPVFYYA
                     SLLPWTYFQGAVTTATNTLIDNQGVVTKIYFPRLIMPVSAIIPGLVDLMIASSILVVI
                     MLIYGVTPAVTAFLAPAFILLAAGTALGVGLWLSVVNAAYRDVRIIIPFVVQAWFFIS
                     PVLYPAESIPRVFRWVYSLNPMAGAIEGFRWSLTGVGNPPGQTALISAVAVMVLLVTG
                     ALYFRRREVMIADVI"
     gene            1447..2697
                     /locus_tag="VNP73_03615"
     CDS             1447..2697
                     /locus_tag="VNP73_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65040.1"
                     /translation="MTTSIEAHGLSKRYRIGGVERYVALRDVITRKLSAPARRFRGEP
                     APAKKEKQTFWALDDISFNIDEGDIVGIIGRNGAGKTTLLKVLTRITRPTKGYADIHG
                     RVGSLLEVGTGFHPELSGRENVYFNGGILGMTKREIDSKFDEIVEFAETGRFIDTVVK
                     RYSSGMQVRLAFSVAAHLEPEILLVDEVLTVGDLKFQRKCIEKMAQVAESGRTILFVS
                     HQMNQIRRLCERVIWIDQGRLHRDGGTARVVSEYESAANALETEGGSREAGGPTQFIG
                     WWLVEGERDRSHELQSLWAPIKVTFLLNVDKPLDSAHIGIGLYDSAGTLVWGIGEDGI
                     KLATGVHELSFSIASMPLKPGHYRWKLSIWEYSKMLDLWDATTELLVATDPVTHWIDQ
                     WAGVMNIPAKFETQPASDDGRQEV"
     gene            2678..3592
                     /locus_tag="VNP73_03620"
     CDS             2678..3592
                     /locus_tag="VNP73_03620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWL65041.1"
                     /translation="MADKKSRYERARSFSRKPLSEQAHIARELGGRNVRHALKRSIDR
                     VLPFASLPRRIEGGAWWLSANDYVGDAIYEGSFERRERAFVERFLQPGMVMVDVGAHH
                     GLYSVIAATRVGPSGVVVSFEPSERERKRLAFHRRINRLSQIKIEPLAIGKEPGEATL
                     YIPSLKNSGFNSLRPSEEMRSAAVPVVVEVTTIDDYLARSPIGRVDLVKLDIEGGELN
                     ALKGATETLAAYKPVILCEVEDERTLPWGYRAEEIIDLLQEKGYRWFVPQPGGRLLPS
                     TARGSEPGTNLVAITADRDAAELMARSK"
     gene            3589..4599
                     /locus_tag="VNP73_03625"
     CDS             3589..4599
                     /locus_tag="VNP73_03625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWL65042.1"
                     /translation="MSDPKVSIVTPTYNPGTRLERCIASVGAQTYENVEHIVVDAEST
                     DGTVELLMAKPNIRWVSEPDQGQADALNKGFRMAEGELLTWINGDDTLEPDAVRYVVE
                     AYERTGAEWIYGNCEMWDERSAHLWDPPHTLSQESFYWGCPIAQQGTFFSKRAFESIG
                     GIDASFDLSMDFDLWLRLLEAGIASTYVDKVLGRFEVHELSKTGSRGYDEFLVEGALA
                     LRRRGSTGPSDLMVGRAAAFRIGRANGSKIAAEMMKKELRRILEAHTEFGDVDVGRAL
                     AGGYAEAADVELRKRRPFGHLAGLRHLARPAPWRYRLTRSSALGNLRVAAARTLERAR
                     RF"
     gene            complement(4635..5315)
                     /locus_tag="VNP73_03630"
     CDS             complement(4635..5315)
                     /locus_tag="VNP73_03630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWL65043.1"
                     /translation="MPRRIDEISLGHPGISMNSIYLSLKHGGGKGRFPIRAAASARMQ
                     IARTAELKLGGRLVLGLSPQLEAGPATDFEPSTVERAVVTMRENSRLETGGWVVLGPG
                     AQLIVAPGGRVALGRGTYISVGATIICRSTVTVGRDGGISWGVLMMDSNFHPVYKNEV
                     PSSVDVPITIGDHVLIGARSVVLKGSTIGDGSIIAAGSVVSGEIPPNVMAGGVPAKVL
                     HENVTWNW"
     gene            5385..6263
                     /locus_tag="VNP73_03635"
     CDS             5385..6263
                     /locus_tag="VNP73_03635"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWL65044.1"
                     /translation="MLPNFLLIGTAKGGTNALHNYLRAHPDIFMPETKELKFFSNEEV
                     WSKGIDWYEGLFPEGYALRGEGSVQYTMYPNFPNVPQRIQDHIQEARFIYLMRDPVDR
                     MRSQFRHLVAIGWEHEPIEKALVRDWPSLEPFRRPEEKALYIHYSMYAMQLEKYFEIF
                     DRDRFLLLTSEALQYDRAATMRKVLSFLSLDPEWTAPVIEREFHKGADRSTLRNPELQ
                     AKIQKIPGYRALTRVTPQRVKRFFAPVIVRKTDPGEQAIEISDSFRTELEDLLRPDVA
                     KLRGFMGTDFDGWGIA"
     gene            complement(6260..>7051)
                     /locus_tag="VNP73_03640"
     CDS             complement(6260..>7051)
                     /locus_tag="VNP73_03640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65045.1"
                     /translation="GGVKAPWRIVRALGWRAAIFEITRLATRSMWMTFNPLARSALKR
                     AKLVIVQNKETREWLPQRYHPKTVIFQNAVMEGAVAPTPNAKPDGYPLACFAGRLNTW
                     KGAAIAIRTIAATRDWHLVLLGEGPERNRLEALAADLRVADRVTFQGHVPHPELFRVM
                     CEEADAFLFPSLHDDAALAVAEAVACGLPVVCLDIGGTPVIAGERAIAVDPRGAPKTV
                     VKELAAGLEQARTWAHVPSEAERVSLPARTRALRAIVAEHLDLDA"
BASE COUNT         1352 a         2080 c         2216 g         1403 t
ORIGIN      
        1 ctgtctgttg ttctttggac ggaacgacgt gaatcagaag ggactcgacc tcctgatcga
       61 ggggtacgcg ctggctcggg cccgaggtct cgacctgccg ctcgtcatcg ggggccgggc
      121 gcacgaggac agcgccaact acttaacgga ggccgtcaag cggcaaggtg tctcggaaca
      181 cgtctcgttc gtcggtgaga ccagtgacca ggagcgctcg cggctgatgt ctaacgcgcg
      241 cgccttcgtc tttcccagcc ggtgggatgg cccgccgaga cccgtgcgcg aggcgatcgc
      301 actcggcgtt cccgtgctgg taacaccggg caccaacatg gccgaggtgg ttgccgagca
      361 cgatgccggg gccgtcatgc agccgacgcc ggagggtgtg agagacgcat tgatccagct
      421 atcggacgtc gaccgtgtcg agcggtggcg gcgcaactcc gaaaagctgc gcgcggctct
      481 cagctggccg aaactcgcgc gcctgtatct cgccgcttac gagggctgac cacctgccgc
      541 tacactttgc agcttcgacc ccggccgtga ggtgagagtg tccgccaccg cgtctagaac
      601 gggatctgct gcgacgggta agtcgactcc ccccataacg aggatccggc ccatcggtgg
      661 ctggttcgac ctgaacctac gcgagttctg ggcgtatcgc gagttgatct atttcttcgt
      721 gtggcgggac ctgaaggtgc gctacaagca aaccttcgtg ggggccgtgt gggtgatctt
      781 gcaaccgacg gcaacgatga ttctcttcac ggtctttttc ggtggtgttt tagggctgcc
      841 gaccggtggc ctccccgatc cggttttcta ctacgcgagc cttctcccgt ggacctattt
      901 ccagggtgca gtgacgacgg cgacgaacac gctgatcgat aaccagggcg ttgttacaaa
      961 gatctatttc ccgcgcctca tcatgcccgt ctcggcaata atcccgggtc tggtcgacct
     1021 catgatcgcc agctcgatcc tcgtcgtgat catgcttatt tatggggtga caccggcggt
     1081 gaccgcgttc ttggcccccg ccttcatttt gcttgccgcc ggaaccgcgc tcggtgttgg
     1141 cttgtggttg tctgtggtca acgccgcgta tcgcgacgtt cgaatcatca tccccttcgt
     1201 ggtccaggcg tggttcttca tatcgcccgt tctttatccg gccgaatcca tccctagggt
     1261 tttcaggtgg gtctacagct tgaacccgat ggcgggggcg atcgaaggct tccgctggtc
     1321 gctcaccggg gtcggtaacc ctcccggcca gaccgcattg atctcggccg tcgccgtgat
     1381 ggttcttctg gtgacgggag ccctctactt ccgtcggcga gaagtcatga tcgcggatgt
     1441 catctgatga ccacatcgat cgaggcccac ggtctctcga agagataccg catcggcggg
     1501 gtggagcgtt acgtagcgct gcgcgacgtc attacgagga agctcagcgc gcctgctagg
     1561 cgttttcggg gcgaaccggc tcctgcgaag aaagagaaac agaccttctg ggcgctggac
     1621 gacatctcct tcaacatcga cgagggcgac atcgtcggca tcatcggtcg aaacggggcg
     1681 gggaaaacga cgttgctgaa agtgctcacg cggatcacac ggcctacgaa aggttacgcc
     1741 gacatccacg gtcgagtggg aagtctgctc gaggtcggca cggggttcca ccccgagttg
     1801 agcggccgcg agaacgtgta cttcaacggc gggatcctcg gcatgacgaa acgagagatc
     1861 gactcgaagt ttgacgagat cgtcgagttc gcggagaccg gccgattcat cgacacggtt
     1921 gtgaagcgct attcgagcgg catgcaggtt cgactcgcgt tctcggttgc cgcccacctc
     1981 gagcccgaga tcctgctggt cgacgaggtg ctgacggtcg gggatctgaa gttccaacga
     2041 aaatgcatcg agaagatggc ccaggtcgcg gagagcggcc gcacgatcct gttcgtcagt
     2101 caccagatga accagatcag gcggctgtgc gagcgggtga tctggatcga tcaaggtcga
     2161 cttcaccgcg acggggggac ggctagggtc gtttccgaat acgagagcgc ggcgaacgcg
     2221 ttggagaccg agggtgggag ccgggaagcc ggtggtccga cacaattcat cggctggtgg
     2281 ctcgttgaag gagagcgcga tcgatcacac gagctgcagt cactatgggc tccgatcaag
     2341 gtcacgttcc tgctaaacgt cgacaagcca cttgactccg ctcacatcgg gatcggcttg
     2401 tacgactcgg ccggcactct cgtgtggggc atcggcgagg atgggatcaa actcgcaacg
     2461 ggcgtccacg agctttcttt ctcgatcgcg tccatgcctc tgaaacccgg ccactaccga
     2521 tggaagctga gcatctggga gtacagcaag atgctcgatc tttgggatgc aacaacggag
     2581 ttgcttgtgg cgacggatcc cgtaactcac tggatcgatc aatgggccgg agtcatgaac
     2641 atcccggcaa agttcgaaac acaacctgca tcggacgatg gccgacaaga agtctaggta
     2701 cgagagagcc agatctttct ctcggaaacc gctctccgag caggctcaca tcgcgcgcga
     2761 gctcgggggc cgcaacgtcc gacacgcgct gaaacgttca atcgaccgcg ttctgccgtt
     2821 cgcttcgtta ccccggcgta tcgaaggggg tgcatggtgg ctctcggcca atgactatgt
     2881 gggcgatgcg atctatgagg gctcgttcga gcggcgtgaa cgggctttcg tcgagcgttt
     2941 tctccaacca gggatggtca tggtggatgt gggtgctcac catggcctct actccgttat
     3001 cgccgccacg cgggtcggcc cctcgggtgt cgtcgtttcg ttcgagccgt ccgagcgcga
     3061 gcggaagcgt ctggccttcc acagacgcat caaccgcttg tcacaaatca agatcgagcc
     3121 attggcgatc ggaaaagagc ccggtgaggc aacgctgtac atcccttcac tcaagaacag
     3181 cggattcaac agccttcgtc cctcagagga gatgcgatcg gcggcagtgc cggttgtggt
     3241 cgaggtgacg acgatcgacg attatctggc tcggtcgccg atcggtcgtg tcgatctggt
     3301 caagctcgac atcgaaggcg gagaactgaa cgcgctcaaa ggagctacgg aaacccttgc
     3361 cgcctacaag ccggtgatcc tgtgcgaggt cgaggacgag cgcaccttgc cctggggtta
     3421 tagggcggaa gagatcatcg acctgcttca ggaaaagggc taccgctggt tcgttccgca
     3481 gccggggggc cggctgttgc cttcgacggc gcgtggatct gagccgggaa cgaaccttgt
     3541 ggctataacc gcagatcggg atgcggcaga gttgatggcg agatcgaaat gagcgatccg
     3601 aaggtatcga tcgtcacccc cacgtacaac ccgggcacga gactggagcg ctgcatcgca
     3661 agcgtcggcg cgcagaccta cgagaatgtc gaacacatcg tcgtcgatgc cgagtccacg
     3721 gatgggactg tcgagctatt gatggcgaaa ccgaacatcc ggtgggtctc ggagcccgat
     3781 caaggccagg ccgatgcctt gaacaaaggc ttccgtatgg ccgaggggga gttgcttact
     3841 tggatcaacg gcgacgacac gctcgagccc gacgcggttc ggtacgtggt cgaggcctac
     3901 gaacgtacgg gagcggaatg gatctacggc aactgcgaga tgtgggatga gcggtcggct
     3961 catctctggg atccacccca cacgctgtca caagagagtt tctattgggg atgtcccatc
     4021 gcccagcaag gaacattctt ttccaagaga gcattcgaat cgatcggagg catcgatgcg
     4081 tctttcgatc tgtcgatgga tttcgacctc tggttgcgct tgctcgaggc cggaatcgcg
     4141 tcgacgtacg tcgacaaggt cttagggcgt ttcgaagtcc acgagctgtc taagaccggc
     4201 agccgcgggt acgacgagtt tcttgtcgaa ggagcgcttg cgcttaggag acggggatcc
     4261 acgggacctt cggatctgat ggtgggtcga gccgccgctt ttcggatcgg gcgcgcgaac
     4321 ggttccaaga tagcggcgga gatgatgaag aaagaactgc gccggatcct ggaggcgcat
     4381 actgaattcg gtgatgtgga cgtcggtcgt gcgttggcgg ggggatacgc cgaagctgcc
     4441 gacgtcgaac tgcgcaagcg tcgcccgttt gggcatctgg cagggctcag gcacctggcg
     4501 cgtcccgccc catggaggta ccgactcacg cgctcgtcgg cgttggggaa tctgcgcgtc
     4561 gctgccgctc gaacgctcga acgagcgcgg cgtttctgaa tcgttcggat tcttcttgtc
     4621 ctaggggtat ccccctacca gttccaggtc acgttctcgt gtaagacctt cgccggtacg
     4681 cctcctgcca tcacgttggg cggtatctcg cccgagacga ccgagcctgc cgcgattatc
     4741 gaaccatctc cgatcgtgga gcctttcaac actacggatc gcgccccgat gaggacgtgg
     4801 tcgccgatgg ttatcgggac gtcgacggat gaagggactt cgttcttgta cacggggtgg
     4861 aagttggagt ccatcatcag tacgccccac gagatccctc catcgcgacc aaccgtcacg
     4921 gtcgatcggc agatgatggt cgctcccacc gagatgtagg tgccgcgccc caacgccacg
     4981 cgtccacccg gtgcgacgat caactgtgct cccggcccga ggacgaccca ccccccggtc
     5041 tccaagcgcg agttctctcg catcgtaacc acggctctct cgacggtaga cggttcgaag
     5101 tcggtcgccg gtcctgcttc gagctgcggc gacaaaccga ggaccaggcg cccgccgagt
     5161 ttcagctcgg cggtgcgagc gatctgcatc cgggccgacg ccgcagctcg gatcgggaag
     5221 cgacccttgc cacctccatg tttcaaagat aagtagatgc tgttcatcga gatgcccgga
     5281 tggccgagtg agatctcgtc gatgcgtctt ggcactacgt tctccagcaa tgagccgtcg
     5341 ggtagcgtga cactatcggg cgggtttctg aaaggtgacc gaggttgctc cctaacttcc
     5401 tactcatagg aaccgccaag ggtgggacca acgcgctgca caattacctt cgagcgcatc
     5461 ccgacatctt catgcccgag acgaaggagc tgaagttctt ctcgaacgaa gaggtctgga
     5521 gcaagggaat cgattggtac gaagggctgt ttccagaggg ctatgccctc cggggcgaag
     5581 gatcggttca atacacgatg tacccgaact tcccgaacgt tccgcaacgg atccaggatc
     5641 acatccagga agcgcgcttc atctacctga tgcgcgatcc cgtcgaccga atgcgttccc
     5701 agtttcggca cctagttgcg atcggctggg aacacgagcc gatcgagaag gcattggtgc
     5761 gcgactggcc gtcgctggag ccgttccgac ggcccgaaga gaaggccctc tatatccact
     5821 acagcatgta cgcgatgcag ctcgagaagt atttcgagat cttcgatcgc gatcggttcc
     5881 tgttattgac gtccgaagcg ctgcaatacg accgggcggc gacgatgcgc aaagttctga
     5941 gtttcctcag cctggatccc gagtggaccg caccggttat tgaacgcgag tttcacaagg
     6001 gagccgatcg cagcacgcta cgcaatcccg agcttcaggc aaagatccaa aagatccccg
     6061 gttacagggc cctgacgagg gtcacacctc agagggtcaa gcggtttttt gctccagtga
     6121 tagttaggaa gaccgacccc ggcgagcagg ccatcgagat ctccgactcg ttccggacgg
     6181 agctcgagga cctccttcga cccgacgtgg cgaagcttcg cggcttcatg ggtaccgact
     6241 tcgacggctg ggggatcgcc taggcatcga ggtcgagatg ctctgcgacg atggctcgaa
     6301 gtgctcgagt tcgagccggc aacgacaccc gttcggcttc gctgggtacg tgggcccacg
     6361 tgcgtgcctg ctcgaggccg gcggcaagct ccttaaccac ggtctttggg gccccgcgcg
     6421 gatcgaccgc gatggctcgt tctcccgcta tgactggggt tccccctatg tcgaggcaga
     6481 ccaccggcag tccgcacgca acggcttcgg cgaccgcgag cgccgcgtcg tcatgcaggc
     6541 tggggaacag gaaagcgtcg gcttcctcgc acatgactcg aaataactct ggatgcggga
     6601 cgtgcccctg gaacgtgact cgatcggcca ctctcagatc ggctgccaag gcctccagcc
     6661 ggttgcgctc cgggccttcg ccgagcaaga cgaggtgcca atcccgggta gccgcgatcg
     6721 ttcgaatcgc gatcgcggct cccttccacg tgttcaggcg cccggcgaaa caggcgagtg
     6781 gataaccatc gggttttgcg ttcggagtag gagcgacggc gccttccatc acggcgttct
     6841 gaaagatgac ggtcttgggg tgatagcgct ggggaagcca ctcgcgggtc tccttgttct
     6901 gcacgatcac gagcttcgct cgctttagcg cgcttcgtgc caatgggttg aaggtcatcc
     6961 acatcgaacg agtggccagt ctcgtgattt cgaatatggc cgctctccaa cccaaagctc
     7021 gaacgatcct ccatggagct ttcacgccgc c
//
LOCUS       DATMED010000144         3060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2421419, whole genome shotgun sequence.
ACCESSION   DATMED010000144 DATMED010000000
VERSION     DATMED010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2421419"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /gene="steA"
                     /locus_tag="VNP73_03645"
     CDS             <1..1037
                     /gene="steA"
                     /locus_tag="VNP73_03645"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HWL65046.1"
                     /translation="GLVQVRPRAVVNAARSATGRYPNLGPLLLCSAGILVIDQAGPAA
                     LGIPEGSRLVIDGADILKVTGGGRDLIATGTVLTLEEAEKTLDKSKQSIATELERFAE
                     NTIGYIREERDVLLEAARLPEVATDFHGRHVLIVVRGYNYKEDLGALSTYIRELDPLL
                     IGVDGGADALLDHGFKPDMIIGDMDSVTTEALLSGAELVVHAYPGGHAPGQERMQALG
                     LDSLLFESGGTSEDIAMLLAYEKGAELIVAVGSHANLIEFMDKGRRGMASTFLTRLRV
                     GPILVDAKGVSRLYHSRVRRSDLVLFMAAALMTVAIIVSISVPLREELGFVWNDTIGD
                     LWFAIRRLFN"
     gene            1038..1982
                     /locus_tag="VNP73_03650"
     CDS             1038..1982
                     /locus_tag="VNP73_03650"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HWL65047.1"
                     /translation="MIDFRFHMISLVAVFLALGLGVLIGTAVLNEELVDRIKNNVQTL
                     QEDKRALQEDVLDLDRQLDADARFADVAEQWLTQDTLTGRRVILLDFAGTDDRVEDNV
                     RTSIETAGGEIAAEITFTEKFALPGVVERDQLALALGSTSDVPAELRSESGDVVGSRL
                     ASAALGQGREGRADAADAAARDLLDTLAEAGFVDLDRDSLGRFVPDRGLFVIAGGSVD
                     RRPFNVPALGLPLAVAIADRGAPVVVAESSDSAWGLVAGIRTDAAEDRLTTVDDVESI
                     SGRIATVLGLVQSLDGMTDHYGTGPGAEEVIPRPTPEE"
     gene            1982..>3060
                     /locus_tag="VNP73_03655"
     CDS             1982..>3060
                     /locus_tag="VNP73_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HWL65048.1"
                     /translation="MGSTPTPSLARGAAITTVATAVSRVTGFVRVIVVAATLGTTFLA
                     NTYQTANTAPNLIFELLAAGVLTSVFVPTFVEYIAKNRREEGWDAANALATVALVAMV
                     ALSLVLALCAPLIMRLLTIGVEKQALRASEIELGSRFLRLFAPQVALYGAGMIMTGAL
                     HAHRRFAMAAIAPIFNNVVVIAVYLAYAAMRGSHSPSVGGITDAEVFVLGAGTTLGVA
                     AMTFCLVPHLAALGWRYRWRFDPRHPAVQKGARLGVWALGYAGGYQAGLIVVLILANK
                     IQGGVAAYQWAFTFFYLPHALFAVPVFNVLFTAMAEHAALGEDESFLERMRDGLGMLA
                     FILIPIAVILMAVSQPLARITLQYGV"
BASE COUNT          521 a          934 c         1020 g          585 t
ORIGIN      
        1 aagggttggt ccaggtgcgg ccgcgggcgg tggtgaacgc cgcgcgatcg gccaccggcc
       61 gctaccccaa tctcgggccc cttctgttgt gctcggctgg gatcctcgtg atcgatcagg
      121 ccggtcccgc cgccctgggc atcccagagg gaagtcgtct ggtgatcgat ggagccgaca
      181 tcttgaaggt cacgggaggg ggccgcgatc tgatcgccac cggcacggta ctcacgctcg
      241 aagaagccga gaagacgctc gataagtcga agcagtcgat cgcaacggag ctcgagcgct
      301 tcgccgaaaa cacgatcggt tacatccgcg aggagcgcga cgtgctgctg gaagcagcgc
      361 gtctccccga ggtcgctacc gatttccacg ggcgacacgt gttgatcgtc gtgcggggct
      421 acaactacaa ggaggacctc ggcgcactga gcacctacat ccgcgagctc gatccactcc
      481 ttatcggggt tgatggggga gccgacgccc tactcgatca cggtttcaag ccggacatga
      541 tcatcggcga catggactcg gtcacgacgg aggctctgct gtccggtgcc gagctcgttg
      601 tgcatgccta ccccggcggg catgctcccg ggcaggagcg catgcaagct ctcgggctgg
      661 actccctcct gttcgagtcc gggggaacgt cggaggacat cgcgatgctt cttgcgtacg
      721 agaagggggc cgagctcatc gttgcggtcg gcagccacgc caatctgatc gagttcatgg
      781 acaagggtcg tcgtgggatg gcctccacgt ttctgaccag actccgggtc ggcccgatcc
      841 tggtcgacgc caagggcgtg agcaggctgt atcactcgag ggttcgcaga agcgatctcg
      901 tcctgttcat ggccgcggca ctaatgacgg tcgcgatcat cgtctcgatc tccgtacccc
      961 tgcgggaaga actcggcttc gtatggaacg acacgatcgg tgatctctgg ttcgcgatac
     1021 gcaggctctt caactagatg atcgacttcc gtttccacat gatctcattg gtcgcggtgt
     1081 tcttggccct cggtctggga gtgctcatcg gcactgcggt tctgaacgag gaactggtgg
     1141 atcggatcaa gaacaacgtt cagacccttc aagaagacaa acgtgcccta caggaagatg
     1201 tcctcgatct cgatcgccag ctcgatgccg acgcccgctt cgcggatgtg gccgagcagt
     1261 ggctgactca agacacgctg accggccgac gggtgatcct gctggacttc gccgggaccg
     1321 acgatcgtgt cgaggacaac gtcaggacca gcatcgagac cgcaggaggg gagatcgcag
     1381 ccgagatcac attcaccgaa aagttcgccc ttccgggggt cgtcgaacgg gaccagcttg
     1441 ccctcgcgct gggctccacc agcgacgttc cggcggaact caggagcgag agcggggacg
     1501 tcgtcggctc tcgcctcgcg tccgcggcct tgggccaggg gagggagggc cgggccgacg
     1561 cggccgacgc ggcggctagg gatctcctcg acacgctcgc cgaggccgga ttcgtcgatc
     1621 tcgatcgcga ctcgctagga cggttcgtcc ccgaccgcgg gttgttcgtg atcgcagggg
     1681 gcagtgtcga tcgcaggccg ttcaacgtcc cggcgctcgg acttcctctc gccgtcgcca
     1741 tcgccgatcg aggcgctccg gtggtcgtcg cggaatcgtc cgacagcgcg tggggattgg
     1801 tggcaggtat acggacggac gcggccgaag atcgcctgac gacggtcgac gatgtggagt
     1861 cgatctcggg tcggatagcg accgtgctgg gcctggtgca gtcgctcgac gggatgacgg
     1921 atcactatgg caccggcccc ggagccgaag aggtgatccc gaggccgacg ccggaagagt
     1981 gatgggttcg acccccaccc catcactagc gcgcggtgca gcgataacca cggttgcgac
     2041 cgcggtgtcg cgggtgaccg gatttgtgcg agtgatcgtc gttgccgcga cactcggaac
     2101 gactttcctt gcaaacacgt atcagacggc caacaccgcg ccgaacctca tcttcgaact
     2161 cctggcggca ggggtcctga catctgtgtt cgtaccgacg ttcgtcgagt acatcgcgaa
     2221 gaaccggcgc gaagagggat gggatgcggc gaacgctctg gcaaccgttg ccctggttgc
     2281 gatggtcgcg ctgtcgctcg tacttgctct gtgcgcgccg ctcatcatga ggttgctcac
     2341 gatcggtgtg gagaagcagg cgttgcgcgc ttcggagatt gagctgggca gccgtttctt
     2401 aaggctcttc gctcctcagg tcgctctcta cggcgcgggg atgatcatga cgggggccct
     2461 gcacgcacac cggcgtttcg cgatggcggc gatcgctccg atcttcaata acgtcgtcgt
     2521 catcgccgtg tacctcgctt acgccgcgat gcgcggatcc cactcgccaa gcgtcggcgg
     2581 gatcaccgac gccgaagtct tcgtgctggg agcggggacg accctcgggg ttgctgcgat
     2641 gaccttctgt ctcgtgccgc acctcgccgc gctggggtgg aggtaccgct ggcgtttcga
     2701 tccccgacac cccgcggtgc agaagggcgc gcgcctggga gtgtgggcac tcggttacgc
     2761 agggggctac caggccgggc tgatagtcgt tctgatcctc gcgaacaaga tccaaggagg
     2821 ggttgccgcg tatcagtggg ccttcacatt cttctatctg cctcacgcat tgttcgcggt
     2881 gccggtgttc aacgtcttat tcaccgcgat ggcggagcac gcggctttgg gcgaggacga
     2941 gagcttcctc gagaggatgc gggacggact ggggatgctg gcattcatac tcatcccgat
     3001 cgctgtgatc ctcatggccg tgtcgcagcc acttgctcgg atcacgcttc agtacggggt
//
LOCUS       DATMED010000145         8472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4329916, whole genome shotgun sequence.
ACCESSION   DATMED010000145 DATMED010000000
VERSION     DATMED010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4329916"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VNP73_03660"
     CDS             complement(<1..1205)
                     /locus_tag="VNP73_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL65049.1"
                     /translation="MATIPEQRFNVMVPMRDGARLSSDLYLPEAGGPWPTIMTRGHYG
                     KTTPLIPTLVKELNAKGYALVTQDVRGRGDSDGEFVPYDHEGRDGHDTIEWIAEQGWC
                     DGNVALFGGSYGARSGWFTALEQPEHLVAMVILVSPSDPFVEFPTSDMTPMMVSWHRM
                     TSGRVLQNTEGVEWMDVYEHLPLIELDERAGFVSETWKEALAHTTLDEHHKKRRYQNR
                     FDELDIPVLHVSGWYDDEQIGTPLNYVGMSKNAKSEKTRKAQRMVMGPWGHAVNTTRK
                     LGEVDFGPHAIIDLWGYAAKWLDSVLGRSTESVPPVRIFVMGTNEWRDESEWPPARAE
                     FTDFFLHSGGSANSRFGDGTLSTKAPGNDEPGDAFTYDPARPVPFVTSPTSSQLGGPD
                     DYSAIEQRG"
     gene            complement(1208..2326)
                     /locus_tag="VNP73_03665"
     CDS             complement(1208..2326)
                     /locus_tag="VNP73_03665"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HWL65050.1"
                     /translation="MEHASRLHEQCLVIDTCGPLGPSIYTQEMLDRIDEMSAAGAYTG
                     LIISEIESMQRAAIPATGPPGFWTGLAASGVDISVVTIGAFGPAPFSFEGAIADLAGY
                     TRLFDSTDRIRKVTSAAEARAAKDDGVFGVILDFQNSTHARNLDDLDLFYDFGLRVIQ
                     LTYNERNLVADGCTERNPSGLSNYGVSFVKRMNELGILVDTSHSSERTTLDALDVSER
                     PTAVTHSFAKALHNHDRGKGDDVIKAVGESGGYFGVLNVPFFITAEPEGTMNHFIAHF
                     ERIVELAGPEHVGIGTDWGQVVPKTLIDLLHEEVRRFGFRPEHRVDFGAQTRGFETWE
                     KWPNITAALVERGYSDNEIKGFLGTNFLRVFERATEGA"
     gene            2508..3452
                     /locus_tag="VNP73_03670"
     CDS             2508..3452
                     /locus_tag="VNP73_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65051.1"
                     /translation="MSPPAAGQTSREEIEVTTSKAAWYAQAPPCTAIDCSVVPLVSPY
                     PEDTVHVAVSGGQETARTYVAFPIPIDDVVGGTLELPLDVDPANGSVSPETANFMACL
                     AATKFEPVRGSFAEPPKTQCRVGKPARYDEKEVAFTVDLARFADHWKKAEAALALVPS
                     AGAVEGGETWHVVFPAGEDQTQEPEEQQPAPEIVATLEYLSEDDPLGTDSTVDLGTDT
                     TTDPSGSIVDLGSSSGPSFDDGTGFTGAQPSTEEVPDVSVPTELSAEEAAPVASFVEG
                     FAGPGFAYPIVWALPLLLLVGLGAVGRALTKELYRRGM"
     gene            3452..3922
                     /locus_tag="VNP73_03675"
     CDS             3452..3922
                     /locus_tag="VNP73_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65052.1"
                     /translation="MSRLRVLGALMVVALMASACAEGLAFVKDDRVEIVAPKGHTKVS
                     LPVTIDWEVEDFDGYFALFVNTTPVPPGKTLEWIARDDQTCLNTPGCPDRTYFADRGV
                     YSTTETSVTLERLPDRDAYQGHETHEVTVILLDGTGRRLTESAWHVTFFYDREV"
     gene            3924..6104
                     /locus_tag="VNP73_03680"
     CDS             3924..6104
                     /locus_tag="VNP73_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL65053.1"
                     /translation="MSEVDQIEQAAQRVRDRARADLGVTGEAEAEPLRKVLREEGISI
                     YPLAALTMLGMMNILFLYAFAVLTPDIGRSLGLSVAAFAGINALDTLAITLSPLPMAW
                     LSQKKPRRALICVVTALLWSVLTIYMGFVVGLIGVLFVVVLDGLSTGSAYALHRPLVM
                     DSYPPKVRMRALSAYSAGDVGLALVMAPLLVAGLSGIFDLTWRGVFVVLGVISLLGSL
                     LAVRLRDPGFGRFDTERVRETVHEVHGEKVEVSAKDVQLGFFEIVRRLMLAPTIRRLT
                     MGFFVLGIFQIPVQTFLAFFLDQRFGLDATQRGLFFACTAFVGMIALLTFAKRGERTF
                     SEDPAQVLKLAGYALAFAVAAISLTALAPSLIWALFSYSLGIAFNVVLFPALYGVLLS
                     VSEARWRPHLSALLGIFLSVGGIFGAFFLSGIDRRFGVGGAMVSLIVPGMAGALIIGT
                     ARRFVNDDLDRLINRVVEEEEIDRIHASGGHLPMLACRSIDFAYGKLQVLFDVDFTVD
                     EGEMVALLGVNGAGKSTLLKAVSGIGLPTKGSVRFQGQDVTYLDAERRLKLGIAQVPG
                     GRAVFPPLTVIENMRVYGYALGRNEKLIDESIDLCLETFPRLAERRNQAASTLSGGEQ
                     QMLGLCKALILKPRLLLIDELSLGLAPVIVGQLLDMVRQINADGTAVVLVEQSVNIAL
                     SVARHAYFMERGQVRFDGSSEDLLARGDLLRAVFLEGAGRMTKG"
     gene            6101..>8472
                     /locus_tag="VNP73_03685"
     CDS             6101..>8472
                     /locus_tag="VNP73_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL65054.1"
                     /translation="MRPPPRAWLIRTGQLAAVMVGLALVLPLLAQLPATTQTGRVETP
                     LPVLSLGAIIGLTYGLLGVGLVLVYRTNRIVNFAHGEIGAFGAAFFGIAVVQWHIPYW
                     IAFPFGLLCGAAVGAAAETGVIRRLRKAPKLMSVVATLGVGQLLVGLALAINSQASAG
                     TVYPEPSFLPSFQIGALLITPAHTGMLIFGPVLVIGLAIFLRRSRHGLALRAAAAAPD
                     TARMSGVFASRMSSLAWALAGGLSAFTAIMTQPTQGFSGAQTFGPSLLLRALAAAAIG
                     RMSSLPGALAAGIGLGVLEQLLLWNFAQSGLVQVVLFGIILIALILQRTRVGREEEES
                     GSWAAVQALRPVPDMLRELWLVRHLGAIVGLITLVGAALLPLVISHSAAVTMSGIFAL
                     AMVGLSLGIVTGLGGQLSLGQFAVGAIGAVVSFYVTSNGGNFVLSFLYAGFVAAVCSL
                     LLGLPALKARGLMLTVTTLSFALIAPAWLLDQPWMLGDGVSPQHPAPFGISLDTGLSY
                     YFFALGALLFSMLIARNVRSSGLGRLLIAVRDNEDNAGAFTVRATLTKSQAFLLAGFI
                     AGIAGALYFHGLSRVTGLSFPTATNIDVVKMTVIGGLGIMSGPLLGALFVLAVPRFVP
                     LDAAALATTSFGQLLIILYLPGGIAQLVEPLRDRVVRFIGRRAGVDVDYAYASATPVG
                     EGGVGTAADEVTGLTPARRRKPPGTLLLEASDLRKSFGGVHAVDGVSLEIFAGETVGL
                     IGPNGAGKTTTFELLSGFLNPDEGVVWFDGQDVTILGPEARAHRGLIRSF"
BASE COUNT         1393 a         2617 c         2703 g         1759 t
ORIGIN      
        1 tcgcctcgtt gttcgatcgc cgagtagtcg tccggccccc ccaactgcga actggtcggt
       61 gacgtgacga aaggaaccgg gcgggccggg tcgtaggtga acgcgtcgcc cggctcgtcg
      121 ttacccggag ccttcgtcga caacgtcccg tcgccgaagc ggctgttggc ggaaccacca
      181 ctgtggagga agaagtccgt gaactccgcg cgagccggcg gccactcgga ttcgtcccgc
      241 cactcgttcg tgcccatgac gaagatacga accgggggaa ccgactcggt gctgcggccc
      301 agcaccgagt ccagccactt cgccgcgtaa ccccacagat cgatgatcgc gtgcggccca
      361 aagtccactt ccccgagctt gcgggttgtg ttcacggcat gtccccacgg gcccataacc
      421 atccgctgtg ccttgcgcgt cttctcgctc ttggcgttct tggacatccc cacgtagttg
      481 agcggcgttc cgatctgttc gtcgtcgtac caaccggaga cgtggagcac ggggatgtcg
      541 agttcgtcga atcgattctg atagcgacgt ttcttgtggt gctcgtccaa ggtcgtgtgg
      601 gccaacgctt ccttccacgt ctcgctgacg aagcccgcgc gttcgtcgag ttcgatgaga
      661 ggaaggtgct cgtagacgtc catccattcg acaccttcgg tgttctgaag cacgcgaccg
      721 gaggtcatcc ggtgccaact caccatcatc ggcgtcatgt ccgaggtcgg gaactcaacg
      781 aaaggatccg agggcgagac gaggatcacc atcgcgacga ggtgctccgg ctgctcgagt
      841 gctgtgaacc agcccgacct ggccccgtat gacccaccga atagcgcgac gttgccgtca
      901 caccagccct gctccgcgat ccattcgatc gtgtcgtgac cgtctcggcc ctcgtggtcg
      961 tagggcacga actctccgtc cgagtcacct cgtccccgga cgtcttgggt gacgagcgcg
     1021 tagcccttcg cgttcagctc tttcaccaag gtcgggatca ggggtgtcgt ttttccgtag
     1081 tgaccgcggg tcatgatcgt gggccacggc ccgccggctt cggggagata aaggtcactc
     1141 gagaggcgtg caccgtcacg catcggaacc atcacgttga accgctgctc cggaatcgtt
     1201 gccacggtca agctccttcg gtcgcccgct cgaatacgcg caggaaattc gtgcccagga
     1261 agcccttgat ctcgttgtcc gagtagccgc gctcgaccag cgcggccgtg atgttcggcc
     1321 acttctccca cgtctcgaag ccgcgcgtct gtgcaccgaa atcgacccgg tgctctgggc
     1381 ggaagccgaa ccgccgaacc tcctcgtgga gcaggtcgat caacgtcttc ggaacgacct
     1441 gaccccagtc ggtgccgatg ccgacgtgct ccggcccggc cagctcgacg atccgctcga
     1501 agtgagcgat gaagtgattc atcgttccct cgggctcggc cgtgatgaag aacgggacgt
     1561 taagaactcc gaagtaccca ccggactcgc cgaccgcctt gatgacgtcg tcgcccttgc
     1621 cccgatcgtg gttgtgcagt gccttggcga acgaatgggt gaccgcggtt ggtcgctcag
     1681 aaacgtcgag cgcatcgagt gtcgtgcgct cggacgaatg actggtgtcg acgagtatcc
     1741 cgagctcgtt catacgtttg acgaacgaga ccccatagtt cgacaacccg ctggggttgc
     1801 gctcggtaca tccgtcggcg acgaggttgc gttcgttgta agtcagttgg atcactcgta
     1861 agccgaagtc gtagaagaga tcgaggtcgt cgaggttccg tgcgtgcgtc gagttctgga
     1921 aatcgaggat gacgccgaac actccgtcat ccttcgccgc tcttgcttca gcggccgagg
     1981 tcacctttct gatccgatcc gtcgagtcga acagtcgtgt gtaaccggcc aggtcggcga
     2041 tcgcgccctc gaaagagaac ggggccggac cgaacgctcc gatcgtgacg acgctgatat
     2101 cgacgccgct ggcggcgagc ccggtccaga agccgggcgg gccggtcgcg gggatcgccg
     2161 cccgctgcat gctctcgatc tcgctgatga tcaaccctgt ataagcacca gcggcactca
     2221 tctcgtcgat ccgatcgagc atctcctgcg tgtagatcga agggccgagt ggaccgcacg
     2281 tgtcgatgac gaggcactgc tcgtgcaggc gggaagcgtg ttccatcacg accgatgcta
     2341 ggagggttcc gccccctagc acttcgtggg cgaaccaaag taggggccgt gctttcgtcg
     2401 ggcgcgacga ggggagtgtg cccgggagaa cgggataatc gcgggtggaa atgaacaaaa
     2461 agcatctatc ccctcttggt tgtgccgtgg ttttgatcat ggcgttcttg agcccacccg
     2521 ctgccggcca gacctcccgc gaagagatcg aggtgacgac ttcgaaggcg gcctggtacg
     2581 cgcaggcgcc accgtgcacg gcgatcgatt gctccgtggt gccgctggtg tccccgtatc
     2641 cggaagacac ggtccacgtt gccgtttcgg gggggcaaga gaccgcacgg acctacgttg
     2701 cgtttccgat cccgatagac gacgtggtcg ggggcaccct cgagctcccg ctcgatgtcg
     2761 atccggccaa cggcagtgtg tctcccgaaa ccgccaactt catggcgtgc ttggcggcca
     2821 cgaagttcga gcccgtaaga ggttccttcg ccgagccacc caagacgcag tgtcgagtcg
     2881 gcaagccggc gcgttatgac gagaaggagg tcgccttcac ggtcgacctc gctcgtttcg
     2941 ccgatcactg gaagaaggcc gaggctgcac tcgcgctggt gccttcggcc ggcgcggtcg
     3001 agggtggcga aacttggcac gtcgtcttcc ccgccggaga ggatcagacc caggaacccg
     3061 aagagcaaca gccggctcct gagatcgtcg cgacgctcga gtacctgagc gaagacgacc
     3121 cgctgggaac ggattccacc gtagatctcg gtacagacac aacgaccgat ccgtcgggct
     3181 ccatcgtcga cctggggtcg agctcgggcc cctccttcga tgacggtacg ggattcaccg
     3241 gagctcaacc gtccacggaa gaagtaccgg acgtatccgt tccgaccgag ctctcggccg
     3301 aggaggctgc tccggtggct tccttcgtag aagggttcgc aggccccggt ttcgcctatc
     3361 ccatcgtgtg ggctctgcca ctgctactcc tcgtcggatt gggggcggta ggccgagcgc
     3421 tcacgaaaga gctttaccgc cgcggcatgt gatgtcccga ctgcgcgttc tgggtgcctt
     3481 gatggtcgtc gccttgatgg ccagcgcatg cgccgaaggg ttggcgttcg tgaaagacga
     3541 tcgggtcgag atcgtggcac cgaaggggca cacgaaagtg tcgctcccgg tgacgatcga
     3601 ctgggaggtc gaggacttcg acggttactt cgcattgttc gtgaacacga ctcccgttcc
     3661 accgggcaag acgctcgagt ggatagctcg cgacgatcaa acgtgtctca acacaccggg
     3721 atgtcccgac cggacgtact tcgccgatcg aggcgtgtac tcgaccacgg aaacgagcgt
     3781 cactcttgag cgtcttcccg accgggatgc gtaccagggt cacgagacgc acgaggtaac
     3841 cgtgatcctt ctcgacggaa ccggccgccg attaacggag agcgcttggc acgtcacctt
     3901 cttctacgac agggaggtgt gatgtgagcg aagtcgacca aatcgagcag gccgcgcagc
     3961 gggtccgcga ccgggcccga gccgatctgg gcgtcaccgg agaagcagag gccgaacctc
     4021 tccgtaaagt cctgcgcgag gaaggcatat cgatctatcc actcgcagct ctgaccatgc
     4081 tcgggatgat gaacatcctg ttcctctatg cgttcgccgt tctgaccccc gacatcggac
     4141 gcagcctcgg tctgagcgtc gccgcgttcg cgggaatcaa cgcgctggac acgcttgcga
     4201 tcacgctgtc gcccttgccg atggcatggc tgagccagaa gaagccgcgc cgcgctctga
     4261 tctgcgttgt cacggctttg ctctggagcg ttctgaccat ctatatgggg ttcgttgtcg
     4321 gtctcatcgg ggttctcttc gttgttgtac tggacggcct ttcgaccggc agcgcgtacg
     4381 cgcttcatcg cccactagtg atggacagct atccaccgaa ggtccggatg cgtgctttgt
     4441 cggcgtactc ggcgggggac gttggattgg ctctggtcat ggccccgctt ctggtcgcgg
     4501 ggctgtccgg catcttcgac ctcacctggc gcggtgtctt cgtggttctc ggcgtcatat
     4561 cgctcctcgg ctcgctccta gcggtgcggt tgcgcgatcc cggctttgga cgcttcgaca
     4621 ccgagagggt gagggaaacg gtgcacgagg tgcacggcga gaaggtcgag gtgtcggcca
     4681 aagacgttca actcgggttc ttcgagatcg ttcgccggct gatgctggcg cctacgatcc
     4741 gccgcttgac gatgggattc ttcgtactcg ggatcttcca gatcccggtc cagacgttcc
     4801 tggcgttctt cctcgatcag cgctttggac tcgacgccac gcaacgtgga ttgttcttcg
     4861 catgcactgc cttcgtgggg atgatcgctc ttctgacctt cgcgaaacgt ggtgaacgca
     4921 ccttcagcga agatcctgcg caggttctca agttggcggg gtacgccctg gcgtttgccg
     4981 ttgccgcgat ctcgctgacc gcattagcgc cgtccctcat ctgggcgctc ttctcttatt
     5041 cgctgggcat cgctttcaac gtagtgctgt tcccggctct ctacggggta ctgctctcgg
     5101 tgtccgaggc ccgctggcgt ccgcacctct ctgcactctt gggaatcttc ctttccgtcg
     5161 gtggcatctt cggagcgttc ttcctttccg ggatcgatcg tcgcttcggg gtcggaggcg
     5221 cgatggtctc gctgatcgtt cccggcatgg ccggggcgtt gatcatcgga acggcgcgcc
     5281 gcttcgtgaa cgacgatctg gatcgattga tcaaccgagt cgtggaagag gaagagatcg
     5341 accggatcca tgcgtccggg ggccacctgc cgatgctcgc gtgccggtcg atcgatttcg
     5401 cctacgggaa gctccaggtg ctcttcgatg tcgacttcac ggttgacgaa ggcgagatgg
     5461 ttgcgcttct gggcgtcaac ggagcgggta agtcgacgct gctcaaagcg gtttccggga
     5521 tcgggcttcc caccaaggga agcgttcggt tccagggcca ggacgttacg tatctagatg
     5581 cagaacgtcg tctgaagctg ggcatcgcac aggttcccgg gggccgcgca gtcttcccac
     5641 ccctgaccgt gatcgagaac atgcgcgttt acggctacgc gcttgggcgc aacgagaaac
     5701 tgatcgacga gtcgatcgat ctttgcttgg agacgttccc tcgcctcgcc gaacgtcgca
     5761 accaagcggc cagcacgctc tcaggcggcg agcaacagat gctgggtctg tgcaaggcgc
     5821 tcatcctcaa gccccgattg ctgctgatcg acgagctgtc actggggctg gcgccggtga
     5881 tcgtcggaca gttgctcgac atggttcggc agatcaacgc cgacggaacg gccgtcgttc
     5941 tcgtagagca gtcggtcaac atcgctctat cggttgcacg ccacgcttac ttcatggagc
     6001 gcggacaggt ccgcttcgac gggtcgtccg aggacttgct tgcacgtggc gacttgctta
     6061 gggcggtctt cctcgagggc gccggacgga tgacgaaggg atgagacccc caccgcgtgc
     6121 atggctgata aggacggggc aattagccgc cgtcatggtc ggcctcgctc tcgtgctgcc
     6181 gttgttggcg caattaccag cgaccacgca aacgggtcgc gtcgagacgc cgctgcccgt
     6241 cctttctctg ggagccatca tcggactgac ctacgggctg ttgggtgtcg gcctcgtact
     6301 cgtctatcga accaaccgga tcgtcaactt cgcgcacggc gagatcggag cgttcggcgc
     6361 agcattcttc gggatcgccg tggtgcagtg gcacatccct tattggatcg cgttcccgtt
     6421 cgggcttctg tgtggtgcgg cagtaggagc ggctgcagag accggtgtga tccgtcgact
     6481 ccggaaggca cccaagctga tgagcgtcgt tgccactctt ggcgtcggtc aactgctcgt
     6541 cggtttggct ctggcgatca acagccaggc gtccgcaggg accgtgtatc cggagccatc
     6601 gttccttccg agcttccaga tcggtgcatt gctgatcacg cccgcacaca caggcatgct
     6661 catcttcggc cccgttctcg tgatcgggtt ggcgatcttc cttcgccgaa gccggcatgg
     6721 attggctctg cgcgctgcgg cagcggctcc cgacacggcg aggatgtcgg gagttttcgc
     6781 gtcgcgcatg tcgtcgctcg cgtgggcgct tgccggggga ctgtcggcat tcactgcgat
     6841 catgacgcag ccaactcaag gtttcagtgg agctcagaca ttcgggccgt ctctgctgct
     6901 tcgggcactt gctgcggctg cgatcgggcg gatgtccagc cttcccggcg cgctggccgc
     6961 ggggatcggc ctcggcgtcc tcgagcagct gttgctctgg aacttcgctc aatccggatt
     7021 ggtccaggtt gttctgttcg ggatcatcct catcgcgctg atcctgcaga gaacccgcgt
     7081 cgggcgcgag gaagaggaga gcggtagctg ggctgcggtt caagcgttgc gtcctgtgcc
     7141 cgacatgttg cgtgagctct ggctggtgcg ccaccttgga gccatcgtgg ggctgataac
     7201 gctcgtcggg gcggccctct tgcctctggt gatctcgcat tcggccgccg taactatgag
     7261 cggcatcttc gccctggcga tggtcgggct ctcactcggg atcgtcaccg gcttgggtgg
     7321 ccagctatcg ctgggtcagt tcgcggtcgg cgcgatcggt gccgtcgttt ccttctacgt
     7381 gacgagcaac ggcgggaact tcgtgttgtc gttcctgtac gcgggcttcg tcgcagccgt
     7441 ctgttcattg ctgctcggct tacccgctct gaaggcgagg ggactgatgc tcaccgtcac
     7501 gaccctgtct ttcgcactca tcgcgccggc gtggcttctc gaccagccgt ggatgctcgg
     7561 agatggagtg tcacctcagc atccggcacc gttcgggatc tccttggaca ccggactgtc
     7621 gtactacttc ttcgcgctcg gggccctgtt gttctcgatg ctcatcgcac gcaacgtccg
     7681 cagcagtggt ctaggccgtc ttctgatcgc agtgcgggat aacgaggaca acgcgggtgc
     7741 attcacggtt agggcgacgc ttaccaagtc acaggcattc ctgctggcgg gcttcatcgc
     7801 cggtatcgcc ggtgccctct atttccacgg cctgtcgagg gtcacgggat tgagctttcc
     7861 tacggcgaca aacatcgacg tggtgaagat gacggtgatc ggcggcctgg gcatcatgtc
     7921 cggcccacta ctcggcgcgc tattcgtgct cgcagtcccg cgcttcgttc cactcgacgc
     7981 ggccgcgctc gccactacgt cgttcggaca gctcttgata atcctgtatc tgcccggagg
     8041 gatcgctcag ttggtcgagc cactgcgcga ccgagtcgtg cgcttcatcg gcaggcgagc
     8101 gggcgtggat gtggactacg cctacgcgtc ggcgacaccc gtaggggagg gaggggtcgg
     8161 caccgcagcc gatgaagtca cgggattgac accggcccgc cggcggaagc cgccggggac
     8221 gttgcttctc gaagcctcgg atctgcgcaa gtccttcgga ggagtgcacg ctgtggacgg
     8281 tgtttctctg gagatattcg cgggtgagac cgtcgggctg atcgggccga acggtgcggg
     8341 caagaccacg acgttcgagc ttctgagcgg gttcttgaac ccggacgaag gcgtcgtgtg
     8401 gttcgacggg caagacgtga cgatcctcgg acctgaagcg cgcgcccatc gcggtctgat
     8461 ccgatccttc ca
//
LOCUS       DATMED010000146         3807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5105469, whole genome shotgun sequence.
ACCESSION   DATMED010000146 DATMED010000000
VERSION     DATMED010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5105469"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /gene="folE"
                     /locus_tag="VNP73_03690"
     CDS             <1..345
                     /gene="folE"
                     /locus_tag="VNP73_03690"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HWL65055.1"
                     /translation="CCEHHLLPFIGQAHVAYIPNADGHITGLSKIARVVDTLSRRPQV
                     QERLTTQIADTIERALNPRGVFVVIEAEHLCMTMRGVRKPGSQTVTSAIRGIFRENEA
                     TRAEAMSLINRG"
     gene            352..1194
                     /gene="folP"
                     /locus_tag="VNP73_03695"
     CDS             352..1194
                     /gene="folP"
                     /locus_tag="VNP73_03695"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWL65056.1"
                     /translation="MKLRCRDHLLPLDQVAVMGVLNVTPDSFSDGGRWFDRDRAIARG
                     VEMGDQGAAIIDVGGESTRPGAADVPEAEEQDRVIPVIQALARHIDVPISIDTRKPAV
                     AAAAVQAGASIVNDTWGERPDPHLIEVAARAEAAYVCMHSRGTPATMRSLTEYSDVVS
                     EVKEWLAGRANDLVGSGLRPDQIVLDPGFGFAKTADQSLELLDRLEEIVAIGYPVLSG
                     TSRKSFIGITLDLGEDERVEGTAATVAISVYKGAKIVRVHDVVQMTRVVKMTEAVCRS
                     RIGG"
     gene            1191..1559
                     /gene="folB"
                     /locus_tag="VNP73_03700"
     CDS             1191..1559
                     /gene="folB"
                     /locus_tag="VNP73_03700"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWL65057.1"
                     /translation="MTGDSISIDGIRAETHIGWPDEERAEAQVVLIDLVLHCDLSRSG
                     LTDDLADTVDYDTVISEVRELVRSSKSRLLEHLAEEIATLISRNPLVERVTVQIRKEA
                     FPGPGAGVEGVSVGIERTFT"
     gene            1556..2041
                     /gene="folK"
                     /locus_tag="VNP73_03705"
     CDS             1556..2041
                     /gene="folK"
                     /locus_tag="VNP73_03705"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWL65058.1"
                     /translation="MTTAFVGIGSNVGDRADFCRRAVQAVEAASGITLVRSSSLYETS
                     PVGGPPQRSYVNLVVKVETDLAPSELLGACQEIERKIGREPSDIRWGPRVVDLDILIF
                     GDEKIVEPHLEIPHPHIRQRRFVLVPLLEIDPGVQDPWGDDYASSLDEAEGDVELLEA
                     Y"
     gene            2117..>3807
                     /locus_tag="VNP73_03710"
     CDS             2117..>3807
                     /locus_tag="VNP73_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HWL65059.1"
                     /translation="MKRLWALLTLALVVTAAAGLPVGARNHMVVVRARGTQAPRFVSG
                     IAVPPMQGSPAFVAHNYLATHGRLFGMDRSGADLRVLDVHRARGTTTVRFGQRVGGHP
                     VWGAQYLVHLDREGEGFAPTTVNGHYFSELIPPSAPRLNVHQALALAKARSRGVDVHE
                     VYRHGLTVLPVNTGRLTYHFSLRGSFLGTPVLHEVFVDAHTGAVAMAYDNLQREGPVV
                     VPGRTSHGDDVNLGAYDRGGVYELRDQSKPMFAARGGEIVTHDAEGSSDYLAKPQTLV
                     TSDQPLFNGADTRSGAVDAHFGASFTYDFFQGFGRDSLDGQGGSIVSTVNATEAGEPM
                     YNAFWDGINKQMVYGNPDPQQYHPFSAALDVVGHELTHGVTQHTGNLAYINQSGAMNE
                     AYSDYFGNAIEVTFEGTSMSDDEAGYVGEGLCKVSNPDFFPCPLRDLNDEQTTEDFIY
                     YLTDLDNGGVHLNSTIYAGALWDVREELEGDADRYIFHALESFTTPLDDFVDGRNSVV
                     AAAQDLAAPPEHIEAIEDAFSGKGIESGWDATSATDATVVLKNVAPVSDIGFSSP"
BASE COUNT          724 a         1155 c         1209 g          719 t
ORIGIN      
        1 tgctgtgagc atcatttgct ccccttcatc ggccaggctc acgtcgctta catcccgaac
       61 gcggacggcc acatcacggg actttcgaag atcgctcgcg ttgtcgacac cttgtcgaga
      121 agacctcagg ttcaggaacg actcacgacc cagatcgccg acacgatcga gagggcgctg
      181 aatccacgcg gcgtgttcgt tgtgatcgaa gccgaacatc tctgcatgac gatgcgaggc
      241 gtccgcaagc ctggaagcca gaccgtcact tcagcgatca gggggatctt ccgtgagaac
      301 gaagcgactc gggcagaagc catgagtctg atcaaccgcg gttagacacc cttgaagctc
      361 cgctgccggg atcacctcct tcctctcgac caggtagcag tgatgggcgt tctcaacgtg
      421 acgcccgact ctttttcgga cggtggtcgc tggttcgatc gcgacagagc gatcgcgcgg
      481 ggcgtcgaga tgggcgatca gggcgctgcc atcatcgacg tcggcggaga gtcgacccgg
      541 ccgggcgcgg ccgacgttcc tgaggctgag gagcaggacc gagtgatccc ggtgatccaa
      601 gccctcgcgc gccatatcga tgtacccatc tcgatcgata cgcgcaagcc cgcggtcgcg
      661 gccgcggcgg tccaggcagg tgcgtcgata gtgaacgaca cttggggcga aagacccgat
      721 cctcacttga tcgaggtcgc ggcccgcgcg gaggctgcgt acgtctgcat gcattcgcgc
      781 gggacgccgg ccacgatgcg atctctaacg gagtattccg atgttgtctc cgaggtgaag
      841 gagtggctcg ccgggcgagc gaacgacctt gtcggatcgg gcctgagacc ggatcagata
      901 gtgctcgacc cgggtttcgg tttcgcaaag acagccgatc agagtctcga gctgctggat
      961 cgtttggagg agatcgttgc gatcggctat ccggttctgt ctggaacttc ccgcaagtcc
     1021 ttcatcggca tcacgctgga tctcggagag gatgagcgtg ttgagggaac ggcggcaacc
     1081 gtcgccatta gcgtttacaa aggtgccaag atcgtgcgtg tgcacgacgt tgttcagatg
     1141 acgagggtgg tcaagatgac cgaggcggtc tgccggtcga ggatcggcgg atgaccgggg
     1201 actcgatcag catcgacggc atccgcgccg aaacccacat cgggtggccc gacgaagaac
     1261 gggccgaagc ccaggtcgtt ctcatcgacc ttgtgctcca ttgcgatcta tctcgatcgg
     1321 ggctaaccga cgatctggca gacacggtgg actatgacac cgtcatctcg gaggtgcgtg
     1381 agctggttag gtcctcgaaa tcgagactcc ttgagcatct ggctgaagag atcgcgactc
     1441 ttatttcacg caaccccctc gtcgagcggg ttaccgtaca gatcaggaaa gaagcgttcc
     1501 caggaccggg tgccggtgta gagggagtgt cggtcggcat cgaaaggacg tttacgtgac
     1561 gactgctttc gtcgggatcg gctcgaacgt cggcgaccgc gccgatttct gcaggagggc
     1621 cgttcaggct gtggaagccg cctcggggat cacactggtt cggtcgtcga gtctctacga
     1681 gacgtcgccc gtcggtggac ccccacaacg ttcttacgtc aacctggtcg tgaaggtcga
     1741 gaccgatctc gctccctcgg agctgctcgg cgcctgccag gagatcgaac gaaagatcgg
     1801 ccgggaaccg agcgacatca gatgggggcc gcgcgtggtc gacctggata tcctcatctt
     1861 cggcgacgag aagatcgtcg agccacatct ggagatccct cacccccaca tcaggcagcg
     1921 tcggttcgtg ctcgtccctc tgctcgagat agaccccggt gttcaggacc cgtgggggga
     1981 cgactacgca tcgtctcttg acgaagccga aggcgacgta gagctcctcg aggcttacta
     2041 ggtccgggtt cgtcgaccga ttaggcagaa ggggctattc cagcccgagc cggaggaaca
     2101 taatttggcc tcttccatga aacgactctg ggcacttctc acgcttgctc tggtagtgac
     2161 ggccgcagcg ggacttcccg tcggcgcgcg caaccacatg gtcgtcgtac gagcgcgggg
     2221 gacccaggct ccgcgcttcg tgtcgggcat agcggtgccc ccgatgcagg ggtcgccggc
     2281 gttcgttgct cacaactatc tggccaccca cggtcgcttg ttcggcatgg atcgatccgg
     2341 ggccgatctg cgggtcctcg atgtccaccg cgcccggggg acgaccacgg ttcggttcgg
     2401 acagcgcgtc gggggccatc ccgtgtgggg agcccagtat ctcgttcacc tcgatcggga
     2461 gggagaaggc ttcgcgccga caaccgtcaa cgggcactat ttctccgagc tgatccctcc
     2521 gtcggccccc cgactgaacg tgcaccaggc gctggcgctg gcgaaagcgc gtagccgggg
     2581 cgtcgacgta cacgaggtct atcgccacgg gttgacggtt ctgcccgtga acaccggacg
     2641 gctgacctac cacttcagtc tccgaggatc cttcttaggg acccccgttc tccatgaggt
     2701 cttcgtcgac gcccataccg gcgcggttgc gatggcctac gacaacctgc agcgtgaagg
     2761 ccccgtcgtc gtgccgggcc gcacgtcgca tggtgacgac gtcaatctcg gggcatacga
     2821 ccgcgggggc gtctacgagt tgcgcgatca gtcgaagccg atgttcgcgg cacgcggggg
     2881 cgagatcgtt acccatgacg cagaaggaag ctccgactac ctggccaaac ctcagaccct
     2941 ggtgacgtcc gaccagccac tgttcaacgg ggccgacact cgaagcggag cggtcgatgc
     3001 gcactttggg gcgtctttca cctacgactt ctttcaaggg ttcggccggg attcactcga
     3061 cggccagggg ggaagcatcg tgtccaccgt caacgccacc gaggcgggcg aacccatgta
     3121 caacgcgttc tgggacggga tcaacaagca gatggtgtac ggcaatcccg atcctcagca
     3181 gtaccacccg ttctctgccg ctctcgacgt cgtcgggcac gagctgactc acggcgtgac
     3241 ccagcacacg ggcaacctcg cctacatcaa tcagtcgggt gcgatgaacg aggcctactc
     3301 ggattacttc ggcaacgcga tcgaggtgac tttcgagggg acgtcgatgt ccgacgacga
     3361 ggccggctat gtcggggaag ggttgtgcaa ggtctctaac ccggactttt tcccttgtcc
     3421 gctgcgtgac ctgaacgacg agcaaacgac cgaggacttc atctattacc tcaccgatct
     3481 ggacaacggt ggagtccatc tgaactcgac gatctatgcc ggagctctgt gggacgtcag
     3541 agaagaactc gagggagacg cggaccgtta catcttccat gcactcgaat cgttcactac
     3601 tccgctcgac gacttcgtcg acggcaggaa ctcggttgtc gcagcggcgc aggatctcgc
     3661 tgcgccgccg gagcacatcg aagcgatcga ggatgctttc agtgggaagg gcatcgaatc
     3721 cggttgggat gcaacgtcgg caaccgacgc gaccgtggtg ttgaagaacg tggcgcccgt
     3781 atccgacatc gggttctcgt ccccaga
//
LOCUS       DATMED010000147         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5479455, whole genome shotgun sequence.
ACCESSION   DATMED010000147 DATMED010000000
VERSION     DATMED010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5479455"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VNP73_03715"
     CDS             <1..925
                     /locus_tag="VNP73_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWL65060.1"
                     /translation="HGRPHEEVTMKVIYATDGFEPALNAGALLEKIGDRSRIDVTVMS
                     VTHAGIPAPKHMPILLDPAPSRRADTLKIVDGAVEKLMAAGFKARGHTVEGHPGQEIV
                     RVVEKDWYDLIVMGSGNSTWLGARLLGSVSSYVLHSSPWSVLIAHEALPEAGKGRVLV
                     GADGSRGAEFTVETLARFADPARTEITVVSVVPANSALLMPVPGSTYNSAEVLEHNER
                     LEREMRDEAQRHADRAATLLRNAGFKAEAHVASGNPTEHLLSEADRGKFDLVAVGSRG
                     LGPFRRTILGSVSDHVARLSRAALVGRRLNT"
     gene            976..>2546
                     /locus_tag="VNP73_03720"
     CDS             976..>2546
                     /locus_tag="VNP73_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWL65061.1"
                     /translation="MPAPHADTADRVVSGLASDPRRGLDETEARARLLSQGPNELRSA
                     PQTPRWRKLLGQFVDPLVLLLLGAVVVSLAVWLIEGSHGVPFETLAIVLIVLLNAVLG
                     YIQEERAERAVAALRRMTTTTVAVMRDDRPRRVPSTELVRGDVILLEEGDAISADARL
                     LDVTALQVAEASLTGESQPVAKQIAPVAEDSVLGDRTNMVFSGTVATFGRGRAVVVET
                     GMDTEIGHIAGMIQRAPDQTTPLQLEIDRVGRLLGVAVVLIAAIVIGTILLTSDVTGT
                     DALVEVMLLGVSLAVAAVPEGLATVLTVVLALGVQRMAGRNAIVKKLAAVETLGSATV
                     VCTDKTGTLTKNEMTVRTIVCASGRVELTGAGYEPTGDILLDGRPLSDGDLRVEVRRA
                     LGAATLASNAQLTNENGIWNVMGDPTEGALLVAARKAGVDADYIDERFKRVGELPFSS
                     ERKLMSTVQTDSENPNLVDVFVKGAPDVLLARCTHEFVGSEAVPLDEARRGTILDGIE
                     ELAGEALRTLGVASRP"
BASE COUNT          453 a          788 c          839 g          466 t
ORIGIN      
        1 ccatggccgt cctcacgagg aggtaacgat gaaggtcatc tatgcaaccg acgggtttga
       61 gccggcgctc aacgccggcg cactgctcga gaagatcggc gacagaagcc gaattgacgt
      121 gaccgtgatg tccgtcactc acgccgggat acccgctcca aagcacatgc cgatattgct
      181 tgacccggct ccctccagac gagcggacac cctcaagatc gtggacggag cagtggagaa
      241 actcatggct gcgggcttca aagcgcgcgg tcacactgtc gagggtcacc ccggtcagga
      301 gatcgtgcgt gtcgtcgaaa aggactggta cgacctgatc gtgatgggtt caggcaacag
      361 cacgtggctc ggcgcccggc tgcttgggag tgtgagcagc tatgtgcttc attcctcgcc
      421 ttggtcggtg cttatagccc acgaagcgct ccccgaggcc gggaagggtc gcgtattggt
      481 aggggccgac ggctctcgcg gggccgaatt caccgtcgag acgcttgcac gtttcgcgga
      541 tcccgcccgg acggagatta ccgtcgtctc ggttgtcccg gccaactcgg ctctcctgat
      601 gccagttccc ggatcgacgt acaactctgc cgaggtgttg gagcacaacg agaggctgga
      661 gcgagagatg cgcgacgagg ctcagcggca cgcggatcgg gcggcaaccc tcttgcgtaa
      721 cgcagggttc aaagccgagg cacacgtcgc ttctggcaac ccgaccgagc acctgctcag
      781 tgaggccgac aggggtaagt tcgatctcgt cgccgtgggc tcccgggggc ttggaccttt
      841 taggcgcacg attcttggat cggtgagcga tcacgtcgca aggctttcgc gcgccgcact
      901 cgtcggtcga cggctgaaca cttgacgtca ctaggaaagc gagagcgtct catctagtcc
      961 gcctaggatt tgctcgtgcc cgcccctcac gcagacactg ccgaccgcgt cgtttccgga
     1021 ctcgcctctg atcctcgacg gggtctggac gagaccgagg cacgggcccg tctccttagc
     1081 cagggcccga atgagctccg gagcgcgcca cagacgcccc gctggcgcaa gcttctcggt
     1141 cagttcgtgg atccgctcgt gttgttgttg cttggagcgg ttgtggtttc ccttgccgtc
     1201 tggttgatcg agggctcgca cggagttccc ttcgagacgc ttgcgatcgt attgatcgtg
     1261 ctcctcaacg cggtcctcgg ttacatccag gaggagaggg cggaacgggc cgtggcggcg
     1321 ctccggcgaa tgacgaccac caccgtcgcc gttatgcgag acgaccgtcc gcggcgcgtc
     1381 ccgtcaactg aactcgttag gggtgacgtc atcctgctcg aggaggggga cgcaatctcg
     1441 gcggacgccc gccttctcga tgtcaccgcg ttgcaggtcg ccgaagcctc ccttacaggt
     1501 gagagccagc ccgtcgccaa gcagatcgcc cctgtcgcgg aggactccgt gcttggtgac
     1561 cgcacgaaca tggtcttcag tgggacggta gccaccttcg gtcgcggcag agcggtcgtg
     1621 gttgaaaccg gcatggacac cgaaataggc cacatcgccg ggatgatcca gagagccccg
     1681 gaccaaacca cgccgctcca gctcgagatc gatcgcgtcg gccgccttct cggtgtagcc
     1741 gtcgtcctga tcgccgccat cgtgatcggc acgatcctgc tcacctccga cgtgacgggc
     1801 acggatgccc tggtcgaggt catgctgttg ggggtttctc ttgcggtcgc cgcggtccct
     1861 gagggccttg cgacggtgct aaccgtcgtc ctggctttgg gcgtccagcg catggcgggg
     1921 cgcaatgcca tcgttaagaa gctcgcggca gtcgaaacgc tcggttcggc caccgtcgtc
     1981 tgcaccgaca agaccgggac tctgaccaag aacgagatga cagtgcgaac gatcgtgtgc
     2041 gcaagcggaa gggtcgagct caccggtgcc ggctacgaac caaccggaga catcttgctg
     2101 gacggaagac cactgagtga tggcgacctt cgcgtcgagg tacggcgggc cctgggagcc
     2161 gcgacgctcg ccagcaacgc tcagctgacg aacgagaacg ggatctggaa tgtgatgggc
     2221 gatccgaccg agggagcgct tctcgtggcg gcacggaagg cgggtgtcga tgccgactac
     2281 atcgacgaac gcttcaaacg cgtcggcgag ctgcccttct catccgagcg gaagctgatg
     2341 agcacggttc agaccgattc ggagaacccg aaccttgtcg acgtcttcgt caagggggca
     2401 cctgacgtgt tgcttgcgcg ctgcacccac gagttcgtcg gctcagaggc ggttccgttg
     2461 gacgaagcac ggcgcggcac gatcctcgac ggcatcgaag agcttgcggg tgaggcgctg
     2521 cggacgttgg gtgtcgccag tcggcc
//
LOCUS       DATMED010000148         3598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1649719, whole genome shotgun sequence.
ACCESSION   DATMED010000148 DATMED010000000
VERSION     DATMED010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649719"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VNP73_03725"
     CDS             <1..331
                     /locus_tag="VNP73_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65062.1"
                     /translation="GTRDFLVYERAIWKLADRANAMGVPHNQLLSAAARDSYYVWEEG
                     STHTEEIGYKSAWWVDVWHLPIEGRYVIVPSPLPGYRTLRQAEYSSWLHADPQHLYLQ
                     TAEGSGS"
     gene            complement(337..1296)
                     /locus_tag="VNP73_03730"
     CDS             complement(337..1296)
                     /locus_tag="VNP73_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWL65063.1"
                     /translation="MSFGLSRIDFEYGLATREAFGEALAEAGSDERIVVVDGDVNNST
                     FTNKFAEKYPKRFFNVGIAESNLVGIAAGFAASGKIPVAASFSVFLMANAFDQIRMSV
                     AFPNLNVKLVGSHAGISIGEDGPSQMAIEDVALACALPGFTVIVPADEASARAATKAM
                     LEREGPTFLRCGRPKVPVIYKDGADFHVGRANTVRDGDDVTIIANGIMVAMALDAAKA
                     LEDEGVEARVLDMHTVKPIDTEAIEKAANETKGIVVAEEHLAFGGLGSIVSMVVAEKK
                     PTRMTFVDLGDTYATSGTPDELLVQYGLTPDAILDAARRVLKD"
     gene            complement(1293..2135)
                     /locus_tag="VNP73_03735"
     CDS             complement(1293..2135)
                     /locus_tag="VNP73_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWL65064.1"
                     /translation="MPQARSEDRTQLEDITRRLRKHIITSTTKAGSGHPSTSLSMVEI
                     LTVLYFGGVLRYDPTNPTDPDRDRFILSKGHGAPGLYAVLAVAGFFPEDDLITLRKLG
                     SPLEGHPNLCRLAGVEASTGSLGQGLSMGIGHALAGKLDDRDYRVYVMVGDGECQQGQ
                     VWEAAMHAGNNALDNLTLIVDRNRHQQTDAVSKVQPLDPLDDKFGDFGWEVSEIDGHS
                     LDEVSAALDWARSVTRKPQAIIANTVKGKGVRLLEEAQAKWHGKPLPPEEEQRALADI
                     GATS"
     gene            complement(2236..>3598)
                     /locus_tag="VNP73_03740"
     CDS             complement(2236..>3598)
                     /locus_tag="VNP73_03740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWL65065.1"
                     /translation="ANDACWYALASPEECPIGREMNLEHPPLGKWLIAAGVKAFGHTI
                     LGHRIVPVIFGVITIPLLYLLARRLLNSTLGASVATGLLAVDFLHLVLSRVAMLDVFL
                     LFFSLLAFWFLLVDRDRRFGEDGERSRVRWLPIAAGAAAGAATASKWSGAFVIVAIAT
                     LALAWDIGARRSPTERHPVLKAIRAEGLTWLVAFLIVPVATYALTFVGRVEGTLLALP
                     TEETAWLNQLWDRQVHIFRFHAHHVFAHRFASEPWSWPLAKRGIPFVRRPDGEMLRFV
                     TAAGNPVVWTLSFVAVIYAGFRWIRNHGPGRPEGFIVAGFAWAYLPWIVYYFAPYLFF
                     TWGRVALFIWYIVPVLPFMYLAMGYVAERVAKRTAGRVAVAAVTLFAVVAFAYYYPVL
                     AYLPVSQQAFRARVALFDQCDPPPHAPFFFFERITEDGVTRFKKRTSPARNLLPPEGW
                     CWL"
BASE COUNT          654 a         1134 c         1169 g          641 t
ORIGIN      
        1 ggggacacgc gactttcttg tgtacgagcg cgcgatctgg aagctggccg atcgagccaa
       61 tgccatggga gtcccgcaca accagctact cagtgccgcg gccagagaca gttactacgt
      121 gtgggaggag ggcagtacac acacggaaga gatcggctac aagtccgctt ggtgggtgga
      181 tgtctggcat ctgccgatcg agggcagata cgtcatcgtg ccttcacctt tgccgggcta
      241 cagaacgctc cgacaggccg agtactcgtc gtggctgcac gccgaccccc agcatctcta
      301 ccttcaaacg gccgaaggct cgggctcgtg acctgtctag tccttcagca cgcgtcgagc
      361 tgcatcgagg atcgcatccg gcgtcaaccc gtactggacg agtagttcgt ccggggttcc
      421 cgacgtcgcg taggtgtcgc ctaggtccac gaaggtcatc cgggtcggct tcttctctgc
      481 taccaccatc gacacgatcg agcccaaacc gccgaaggcg aggtgctctt cggccaccac
      541 gatgcctttg gtttcgttcg ctgctttctc gatcgcttcc gtgtcgatcg gtttgaccgt
      601 gtgcatgtcg agaacgcggg cttccacacc ctcgtcctct aaagccttgg cggcatcgag
      661 ggccatcgcg accatgatcc cgttggcgat gatcgtgacg tcgtctccgt cgcgcacggt
      721 gttcgcgcgt ccgacgtgga agtcggctcc gtctttgtag atcaccggaa cctttgggcg
      781 cccgcagcgc aggaacgtgg gcccctcgcg ctcgagcatc gccttggtgg cagctcgggc
      841 gctggcttcg tcggccggaa cgatgaccgt gaaaccgggg agggcacagg ccagagcgac
      901 atcctcgatc gccatctgcg aggggccgtc ttcaccgatc gagatgcctg cgtggctgcc
      961 tacgagtttc acgttcagat tcgggaacgc gaccgacatg cggatctggt cgaacgcgtt
     1021 cgccatcaag aagaccgaga acgacgccgc gaccgggatc ttgccggagg cggcgaaccc
     1081 ggccgcgatc ccgacgaggt tggactccgc gatgccgacg ttgaagaacc gcttcgggta
     1141 cttctcggcg aatttgttcg tgaacgtcga gttgttgacg tcaccgtcga cgacgactat
     1201 ccgttcgtcc gacccggctt ccgcaagagc ttcgccgaag gcctcgcgcg tggcgagtcc
     1261 gtattcgaag tcgatccgcg acaggccgaa gctcatgacg ttgctccgat gtccgcgagg
     1321 gcgcgttgtt cctcttctgg cgggaggggc ttgccgtgcc acttggcctg agcttcttcg
     1381 agcaacctga ctcccttccc cttgaccgtg ttggcgatga tcgcctgcgg cttgcgtgtc
     1441 accgatcggg cccagtcgag ggcggcagag acctcgtcga gcgagtgtcc gtcgatctcg
     1501 gacacctccc agccgaagtc tccgaacttg tcgtcgagag gatccagcgg ttggaccttg
     1561 ctgaccgcat cggtctgttg gtgccggttg cgatccacga tgagagtcag gttgtcgagg
     1621 gcgttgttac ccgcgtgcat cgcggcttcc cacacctggc cctgctggca ctccccatcg
     1681 ccgaccatca cgtagacgcg gtagtcgcgg tcgtcgagtt tgcccgccag agcgtgcccg
     1741 atccccatcg atagtccttg ccccagtgac ccggtggagg cctcgacccc ggccagccgg
     1801 cacaggttgg ggtgcccctc gagaggcgag cccagcttgc gcaaggtgat caggtcgtcc
     1861 tccgggaaga agccggcgac ggcaagaacg gcatagagac ccggggcgcc gtgacctttc
     1921 gacaggataa agcgatccct atccgggtcg gttgggttcg tggggtcgta gcgcaacact
     1981 ccgccgaagt agaggaccgt gaggatctcg accatcgaca aagacgtgga cggatggccg
     2041 gagccggcct tggtcgtcga cgtgatgatg tgtttccgca ggcgtcgtgt gatgtcttcg
     2101 agttgcgttc ggtcttcgct gcgagcctgc ggcatccggc ctccccgttg gtctttctga
     2161 gcgtgctcgc tcctaatcca accacagggg agtggttcga ctctgggcgc gggtggcaga
     2221 actttcgcgc tctcgctaga gccagcacca gccttccggg gggagcagat tgcgggcggg
     2281 gctcgtgcgt ttcttgaacc gcgtcactcc gtcctccgtg atgcgttcga agaagaagaa
     2341 cggtgcgtgc ggtggcggat cgcactggtc gaagagtgcg acccgcgctc ggaaggcttg
     2401 ttgagacacc ggcagataag ccaggacagg gtagtagtac gcgaacgcaa ccacggcgaa
     2461 gagtgtgacg gctgctaccg cgacccggcc tgccgttcgc ttcgcgacgc gctccgccac
     2521 gtaacccatc gcgagataca tgaacggcaa caccggaacg atgtaccaga tgaacagcgc
     2581 gacgcgaccc cacgtgaaga acagatacgg cgcgaagtag tagacgatcc acggtagata
     2641 ggcccacgcg aaacccgcca cgatgaaccc ctcgggacgc cccgggccgt ggttccggat
     2701 ccatctgaaa ccggcgtaga taacggccac gaacgagagg gtccagacga cggggttgcc
     2761 ggccgcggtc acgaagcgaa gcatctctcc gtccggccga cgcacgaaag ggatgccgcg
     2821 cttggctagg ggccagctcc acggctccga tgcgaaccgg tgcgcgaaca cgtggtgggc
     2881 gtggaaccga aagatgtgca cctgccggtc ccacagctgg ttcagccacg ccgtctcctc
     2941 ggtcgggagg gcgagcagcg tcccctcgac gcggcccacg aacgtcagcg cgtaggttgc
     3001 gaccggaacg atcagaaaag cgacgagcca cgtcaagcct tcggcccgga tcgccttgag
     3061 aacgggatgt ctctcggttg gggagcgccg cgctccgatg tcccaggcga gcgcgagggt
     3121 tgcgatcgcc acgatgacga aggctccgct ccacttggat gctgtagcgg cccccgcggc
     3181 cgcaccagcc gcgattggca gccagcgcac gcgggatctc tcgccatctt cgccgaatct
     3241 tcggtcccga tcgacgagca agaaccagaa cgcgagtagc gagaagaaga gcaggaacac
     3301 gtcgagcatc gctacgcgtg acagcacgag gtgcaagaag tcgactgcca gcaatcccgt
     3361 cgccaccgat gcgccgaggg tcgagttgag aagccgccgc gccagcaggt acagcagagg
     3421 gatcgtgatg accccgaaga tcacggggac gatccggtgg ccgaggatcg tgtggccgaa
     3481 ggccttgacc cccgcggcga tgagccattt gcccagcggc ggatgctcga ggttcatctc
     3541 gcggccgatc gggcactcct cgggtgacgc cagcgcgtac cagcacgcgt cgttggca
//
LOCUS       DATMED010000149         2374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3946967, whole genome shotgun sequence.
ACCESSION   DATMED010000149 DATMED010000000
VERSION     DATMED010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946967"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /gene="hisS"
                     /locus_tag="VNP73_03745"
     CDS             <1..1067
                     /gene="hisS"
                     /locus_tag="VNP73_03745"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWL65066.1"
                     /translation="NKQMYTFTDLGGRSLTLRPELTAPVVRAVLERGLHRGPLPVKLC
                     YAGPMFRQERPQKGRYRQFSQLGVEAIGSDDPKVDAEVIEIGNRFLASAGTEPRLLLN
                     SIGHLDDSCRSGYLRQLEAFLKDRADDLAAEDRARVATNPLRTFDSKEAQTIETMKEA
                     PIITEHLCDSCRKHFEAVQDLLTHVGVEFTLQPSLVRGLDYYTRTAFEFVSDRLGAQD
                     ALGGGGRYDGLAEALGGDPLPGIGFALGLDRILLAGGGAATGRSPVKVFVVAMGEDAG
                     REGFRIATELRRRGIGADLDLAGRSMKGQMKAADRAEAIWAVIIGDEEMVARRATLKH
                     LETGEQQSVPFDEILETVSR"
     gene            1064..>2374
                     /gene="aspS"
                     /locus_tag="VNP73_03750"
     CDS             1064..>2374
                     /gene="aspS"
                     /locus_tag="VNP73_03750"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWL65067.1"
                     /translation="MMRSHRCGDLRPEHVDQTVTLCGWVHTRRDHGGVTFIDLRDTAG
                     LVQVVFDASVSDSAHDRAQELRSEYCIRVTGSVHARREGTVNPRLATGEVEVAATEIE
                     ILSRSETPPFQIDEHSEVEESLRLRYRFLDLRRDRLNQNMKLRHQIVAAIRHFFDDEG
                     FVEIETPMLTRSTPEGARDYLVPSRVHAGTFYALPQSPQLFKQLFMVAGLDRYYQIVK
                     CFRDEDLRADRQPEFTQLDLEMSFVDVDDVLEVTERLFKKVWRDVLHVELEDFPRIGY
                     QESMDRFGTDKPDLRFDMELKDLGTTFATTEVRVFRSVLDAGGAVKAIVVPGKGTLGR
                     KELDSIVDEAKALGAGGLVWIACVEESESDTGFKSPIDKFFTPAERGDLLTAMGAATG
                     DLILIVADQKLSTVYKVLGGLRTRLAERFDLIPKLERSDPQAWRF"
BASE COUNT          447 a          686 c          805 g          436 t
ORIGIN      
        1 tcaacaagca gatgtacacg ttcaccgatc ttgggggccg atcgctgacg ctgcggcccg
       61 agttgaccgc accggtagtg cgcgccgttc tcgagcgagg tctgcatcgg gggccgctcc
      121 cggtgaagct ctgttacgcg gggcccatgt tcaggcaaga gcgtccccag aaaggtcgct
      181 accgtcagtt ctcgcaactg ggcgtcgagg cgatcggttc ggacgatccc aaggtcgacg
      241 ccgaggtgat cgagatcggc aaccggttcc ttgcatcggc gggaacggag ccccggcttc
      301 tcttgaactc gatcggccat ctggacgact cgtgccgatc gggttacctg cgacagctcg
      361 aggccttcct caaggatcga gcggacgacc tcgcggccga ggatcgggcg cgcgtggcca
      421 cgaacccgct gcgaacgttc gattcgaaag aggctcagac gatcgagacg atgaaggaag
      481 cgccgatcat cacggaacat ctgtgcgact cgtgtcgtaa gcacttcgag gccgttcagg
      541 acctgctcac ccacgtaggc gtcgagttca cacttcaacc cagcctcgtg cgtggactcg
      601 attactacac gaggaccgca ttcgagttcg tgtccgacag acttggtgcg caggacgcgc
      661 tcggcggtgg gggccggtac gacggtttgg cggaagccct cggtggcgac ccccttcccg
      721 gtatcggctt cgcactcggt ctcgaccgga tactcctggc cgggggcggg gcagcaaccg
      781 ggcgatcacc cgtgaaggtg ttcgtagtcg cgatgggtga ggacgcgggg cgggagggat
      841 tccggatcgc gaccgagctg agacgccgcg gcatcggagc cgatctcgat ctggcagggc
      901 gttcgatgaa ggggcagatg aaagctgcgg atcgggcaga ggccatctgg gccgtgatca
      961 tcggcgacga agagatggta gccaggcgtg ccaccttgaa gcacctcgag acgggggagc
     1021 aacagagcgt cccattcgac gagatcttgg agacggtaag cagatgatgc gttcacaccg
     1081 gtgcggggac cttcgtcccg agcatgtcga tcagaccgtg accttgtgcg ggtgggtcca
     1141 cacccgaagg gaccacggtg gcgtcacgtt catcgacctt cgtgacacgg ccggactggt
     1201 gcaggtggtg ttcgacgcga gcgtttccga ttctgcccac gatcgggcgc aggagctccg
     1261 tagcgagtac tgcatccggg tgaccggctc cgtgcatgct cggcgcgagg gaacggtcaa
     1321 ccctcggctc gccaccggtg aggtcgaagt tgccgccacc gagatcgaga tcttgtcccg
     1381 atcggagacg ccgccgttcc agatcgacga acacagcgag gtggaggagt cactgcggct
     1441 ccgctaccgc ttcctggatc ttcggcgcga tcggttgaac cagaacatga agctgaggca
     1501 ccagatagtc gccgcgatcc ggcatttctt cgacgacgag ggcttcgtag agatcgagac
     1561 cccgatgctg acccggtcta cgccggaggg agcgcgcgac tacctcgtgc cgtcccgcgt
     1621 ccatgcgggg acgttctacg cgctgccgca atcgcctcag ttgttcaagc agctcttcat
     1681 ggtcgcagga ctcgatcgct actaccagat cgtgaaatgt ttccgggacg aggacctgcg
     1741 ggccgaccgc cagcccgaat tcacgcagtt ggatctcgag atgtccttcg tcgacgtcga
     1801 cgacgttctc gaagtgaccg agaggttgtt caagaaggtg tggcgagacg ttctccatgt
     1861 cgagttggag gacttcccgc gcatcggtta tcaggaatcg atggatcgtt tcgggaccga
     1921 caagcccgat cttcgtttcg acatggagct gaaggacctg ggtacgacgt tcgcgacgac
     1981 cgaggttcgg gtgttccggt ccgtgttgga tgcaggcggc gcggtcaagg cgatagtggt
     2041 gccgggcaag gggacgttag gtcgcaagga actggactcg atcgtcgacg aggcgaaagc
     2101 cctgggggcc ggaggtctcg tttggattgc atgcgtcgaa gagagcgaga gcgacacggg
     2161 ctttaagtca ccgatagaca agttcttcac cccggccgaa cgaggagatc tgctgacggc
     2221 gatgggggcg gccacggggg acctgatcct gatcgtggcc gatcagaagc tctccaccgt
     2281 ctacaaggtg ttgggcgggc tgcgaaccag gttggcggaa aggttcgatc tgatcccgaa
     2341 gctcgaacgt tccgatcctc aagcgtggcg gttc
//
LOCUS       DATMED010000150         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2426413, whole genome shotgun sequence.
ACCESSION   DATMED010000150 DATMED010000000
VERSION     DATMED010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426413"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VNP73_03755"
     CDS             <1..364
                     /locus_tag="VNP73_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65068.1"
                     /translation="FSLHELTARLRALMRRSEHAASDVVQVGDLVMDLAAHRVSRAGQ
                     TVELTPKEFDLLEFFMRRRGEVLTRTAILDHVWDFAYDGASNVVDVYVRYLRAKVDRP
                     FGVSSIETVRGVGYRLKP"
     gene            449..760
                     /locus_tag="VNP73_03760"
     CDS             449..760
                     /locus_tag="VNP73_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65069.1"
                     /translation="MPDQTRVVLFGRGRGLSPELCGALGDVDLERISDEPIVDSMKEL
                     GARKPTVVILDGAGPTEDATLDKLRAVAPQAWFLDGAGIVRRRPEGCPAHRSVTKPFE
                     L"
     gene            complement(765..1400)
                     /locus_tag="VNP73_03765"
     CDS             complement(765..1400)
                     /locus_tag="VNP73_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWL65070.1"
                     /translation="MAFPRQLLADHEKIILDLRPHWVAVLPSVFWTIAILVAMFLGYW
                     AVGSIGAIGDTVGKSIVGAIALIVWIFLALLRVLRWQFTMFVLTTDRLITRQGIIAKS
                     SKEIPLESINDVAFNQSVIERLLGAGDLMIESAGTRGQNLISNVRHPEQVQLSIFKEI
                     ESNTARTFRPAPAEPTEDIPSQIEALARLKDQGAITGAEFETKKAELLKRL"
     gene            1455..1748
                     /gene="gatC"
                     /locus_tag="VNP73_03770"
     CDS             1455..1748
                     /gene="gatC"
                     /locus_tag="VNP73_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWL65071.1"
                     /translation="MPIDRAAVDHVARLARLDLDDDERKRMQEELTQILGHVEMIQSL
                     ELDSVEPTTHALPLTNVFREDEVKPSLTPEEALANAPEKEDGRFRVPRILEES"
     gene            1749..3236
                     /gene="gatA"
                     /locus_tag="VNP73_03775"
     CDS             1749..3236
                     /gene="gatA"
                     /locus_tag="VNP73_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWL65072.1"
                     /translation="MSDLIWATATELADRLEKGEVSSVEITQALIDRGAVVDERLHAF
                     LEPTGEIALEQAKAADERRKAGQARSRFDGVPVAYKDIFTTKGTPTTCGSKILAGWVP
                     PYDATVVERCAGAGMPMLGKLNMDEFAMGSSTENSAYGTSLNPWDLECVPGGSSGGST
                     ALVAAGGAPWAWGTETGGSVRQPASLCGLVGVKPTYGLVSRYGMIAFASSLDQAGPIA
                     RTVTDAAALLQTAAGYDPMDATSYSDPVPDYLDGIDGGVDKMRIGIVKEFEGEGSAPG
                     VRDSLREAYTRLEKLGATLEEISLPSFQFGIPAYYLIAPAEASANLARYDGVRYGFRA
                     PGDDVVQMMSRSRDEGFGNEVKRRIMLGTYALSAGYYDAYYGKAQKIRTLIVRELERA
                     YSDVDLIVCATSPTTAFKVGEKASDPLSMYLSDIFTVPVNLAGNAAISVPCGVSSDDG
                     LPVGLQIIARSLDEKTMFQAAYAFEQDLGWAGSDASRPAAVEGAG"
     gene            3237..4706
                     /gene="gatB"
                     /locus_tag="VNP73_03780"
     CDS             3237..4706
                     /gene="gatB"
                     /locus_tag="VNP73_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWL65073.1"
                     /translation="MTATDTDVYETTIGLEIHVEMSTASKMFCGCAVAFGGEPNSRTC
                     PVCLGLPGSLPVPNKQAIEHTMKIGLALDCRIAERSLFHRKNYFYPDMPKNYQISQYD
                     FPLCEQGYLDISGDFGSTRVGITRVHLEEDTGKSVHIGGSGRIGDSEYSLEDFNRAGT
                     PLVEIVTEPDMHSAEEARVFVAELRALLEGLGVSDVRMEEGSLRVDANVSVRRIGETE
                     HGAKVEVKNMNSIRSVGRALEYEEVRQRKALDAGESLVQETRHFEEKAGTTSSGRLKE
                     YSSDYRYFTEPDLVPLSPDRTWIDEIERSLPETPAERRVRFAQHYGLNEYDIGVLTAS
                     KKTSEWFEAAAEAYTGEAKKVVNWITADLFGLLNEAGQELHESKIAPAQLARLVQLVD
                     DGTVSGKQAKTVLGKMFATGSDPADIAAEEGLSQVSDANLIESFVEEVIAENAEAADK
                     VRAGNMGTIGFLVGQVMKKTKGQANPQMVNELLRSKLSS"
     gene            4720..5175
                     /locus_tag="VNP73_03785"
     CDS             4720..5175
                     /locus_tag="VNP73_03785"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWL65074.1"
                     /translation="MTIEMAETMPGPPEVVWRLITDWEHQDDWMLEASDFVVTSDIRE
                     GVGVEAEATITIAGIKTRDKVSVVGWEPQHRLAIRHLGWVAGVGEIFLTPLEGGRTYL
                     FWREDLEPPLGIVGRVGLGLLKPLMRRIFVRDLRILASLTRAATSTALL"
BASE COUNT         1005 a         1576 c         1801 g          968 t
ORIGIN      
        1 gttctcgtta cacgaactga ccgcgcggct gcgcgcgctg atgagacgtt cggagcatgc
       61 tgcaagtgac gtcgttcagg tcggcgacct ggtcatggac cttgcggccc accgcgtgag
      121 ccgggccggg cagacggtcg agttgacccc caaggagttc gacttactcg agttcttcat
      181 gcgcagacga ggcgaggtgc tcacccgaac ggcgatcctc gatcacgtct gggatttcgc
      241 gtacgacggg gcctcgaatg ttgtggacgt atacgtcagg tacctgaggg cgaaggtcga
      301 tcgaccgttc ggtgtgtcgt cgatcgaaac cgtccgcggc gtcggttatc gcctgaagcc
      361 gtaactcccg atcgacttct aatcgctttc tcatccttcc tgggcatgat cccggtggcg
      421 gatcgacggg tggacgaaag gcacgggcat gccggatcaa acgcgagtgg ttctgttcgg
      481 tcgcggtcgt ggtctgtctc cagaattgtg tggagctctt ggggatgtgg atctagaacg
      541 gatttcggac gagccgatcg tcgattcgat gaaggagctg ggcgcccgga agccgaccgt
      601 cgttatcctg gacggcgcgg gtccgaccga ggacgcaacg ctcgacaagc tccgcgccgt
      661 cgcaccgcaa gcctggttcc tcgatggtgc ggggatagtg cgtcgccggc ccgaaggatg
      721 cccggctcat cggtccgtaa ctaagccttt cgagctgtaa agctttagag cctctttagg
      781 agctcggcct tcttggtctc gaactccgct cccgtgatgg cgccctggtc tttgaggcgc
      841 gctaatgcct cgatctgaga ggggatgtct tctgtcggtt ccgcgggggc cggacggaag
      901 gtgcgggcgg tgttcgattc gatctccttg aagatcgaca gttgtacctg ctccggatgg
      961 cggacgttgg agatcaggtt ctggccccgc gttcccgcgg actcgatcat caggtcgcct
     1021 gctccaagta gacgttcgat caccgactgg ttgaacgcaa cgtcgttgat gctctcgagg
     1081 gggatctcct tggagctctt ggcgatgatc ccttgccgcg tgatcaaacg atccgtagtc
     1141 agtacgaaca tcgtgaattg ccagcgaagg acacgaagta gggctaagaa gatccagacg
     1201 atcagcgcga tcgcgccgac gatcgatttc cccacggtgt cgccgatggc cccgatgctt
     1261 cctactgccc aataacccag gaacatcgcg accaggatgg cgatcgtcca gaagacggag
     1321 ggaaggaccg cgacccaatg gggccgcaga tccagaatga tcttttcgtg atcggcgagc
     1381 agctggcgtg ggaaggccac ggagcaaagt tagccctcta ggatgagcgg cgcacccccc
     1441 aaggaggaga aatagtgccg attgatcgtg ccgccgtcga ccatgtcgct cgactggcgc
     1501 gcctggacct ggacgacgac gagcgcaagc ggatgcagga ggagctgacc cagatcctgg
     1561 ggcacgtcga gatgatccag tccctcgagc tggactccgt ggagccgacg acacacgccc
     1621 ttcctctcac gaacgtcttc cgcgaagacg aggtgaagcc gagcctcacg ccggaagaag
     1681 cgctggcgaa cgctccggag aaggaagacg gacgcttccg agtgcctcgc atcctcgagg
     1741 agtcctaggt gtcggatctc atctgggcaa ccgcgaccga gcttgccgac cggctcgaga
     1801 aaggcgaggt ctcgtcggtc gagatcaccc aagccttgat cgatcggggg gcggtggtag
     1861 acgagcgcct ccacgccttc ctcgagccca ccggcgagat cgcgctcgag caggcgaaag
     1921 cggcggacga acgccgcaag gcggggcagg cccgctcgcg cttcgacgga gtgcccgtcg
     1981 cctacaagga catcttcacg accaagggca cgcctacgac gtgcggctcg aagatcctcg
     2041 ccggatgggt gccgccttac gacgcgaccg ttgtcgagcg gtgcgccggc gcggggatgc
     2101 cgatgctcgg caagctcaac atggacgagt tcgcgatggg gtcgtcgacc gagaactccg
     2161 cttatggaac gtcactcaat ccgtgggatc tcgagtgcgt gccgggcggg tcgagcggag
     2221 gttctacggc attggttgcc gctgggggcg cgccgtgggc gtgggggacc gagaccggtg
     2281 gctcggttcg acagcctgca tcgctgtgcg gtctcgtcgg cgtaaagccg acctacggac
     2341 tcgtgtctcg ctacgggatg atcgcgttcg cttcgtcact cgatcaagcc ggaccgatcg
     2401 cacgaactgt gaccgacgcc gcggcgctgt tgcagacggc ggcgggctac gacccgatgg
     2461 acgcgacctc ctactccgac ccggtgcccg attacctgga cgggatagat ggaggcgtcg
     2521 acaagatgcg catcgggatc gtcaaggagt tcgagggcga aggatcggcg cccggtgtgc
     2581 gtgattcgct gcgggaggct tacacgcggc tcgagaagtt gggagcgacg ctcgaggaga
     2641 tctcgctgcc gtcgttccag tttgggatcc cggcctatta cttgatcgct cccgcagaag
     2701 cgtctgcgaa cctcgcacgg tacgacggcg tgcgttacgg ctttcgagcc ccgggtgacg
     2761 acgtcgttca gatgatgtcg aggtcgcgcg acgagggatt cgggaacgag gtcaagcgca
     2821 ggatcatgct ggggacgtac gcgctctccg cgggttacta cgacgcgtat tacggcaagg
     2881 cgcaaaagat acggacgctg atcgtccggg agctagagcg tgcttacagc gacgtcgacc
     2941 tgatcgtgtg cgcaacctct ccgacgacgg ctttcaaggt gggggagaag gcctcggacc
     3001 cgctgtcgat gtatctgtcg gacatcttca cggtgccggt gaacctggca ggcaacgcgg
     3061 cgatcagtgt gccgtgcgga gtgtcctcgg acgacggttt gccggtggga ctgcagatca
     3121 tcgctcgctc gctcgacgag aagacgatgt tccaagcggc gtacgcgttc gagcaagacc
     3181 ttggttgggc cggcagtgac gccagccggc cggcggccgt cgagggggca ggttagatga
     3241 ccgctaccga caccgacgta tacgaaacga cgatcgggct cgagatccac gtcgagatgt
     3301 cgacggcctc caagatgttc tgtgggtgcg ccgttgcgtt cggaggcgag ccgaactcac
     3361 gcacatgccc ggtgtgcctc ggcttgccgg gatcactgcc ggtccccaac aagcaggcga
     3421 tcgagcacac gatgaagata ggtctggctc tggattgccg gatcgccgag cggtcactgt
     3481 tccaccgcaa gaactacttc tatcccgaca tgccgaagaa ctaccagatc agccagtacg
     3541 acttcccgct ctgcgagcag gggtacctcg acatctcggg cgacttcggc tcgacgcggg
     3601 tcggcatcac gcgcgtgcac ctcgaggaag atacgggcaa gtccgtgcac atcggtggct
     3661 cgggccggat cggtgattcc gagtactcac tcgaagattt caaccgcgcc ggcacgccac
     3721 tggtcgagat cgtcaccgag cccgacatgc actcggccga ggaagcgagg gtcttcgttg
     3781 ctgagttgcg ggccctcctc gagggattgg gcgtttcgga cgtgcggatg gaagaaggtt
     3841 cgctgcgggt cgacgccaac gtttcggtga ggcgcatcgg ggagaccgag cacggggcga
     3901 aggtcgaggt taagaacatg aactcgatcc ggtccgtcgg tcgagcactc gagtacgaag
     3961 aggtccggca gcgcaaagct ctggatgcgg gggaatcgct cgtacaggag acgcgtcatt
     4021 tcgaggagaa ggcggggacg acgtcgtccg ggcgtttgaa ggaatactcg tccgactacc
     4081 gctacttcac cgagcccgat ctcgtgccgc tcagtccgga ccgcacctgg atcgatgaga
     4141 tcgaacggtc gttgccggag acacccgcgg agcgtcgagt gcggttcgcg cagcattatg
     4201 ggctcaacga atacgacata ggtgtgctca ccgcctccaa gaagacctcg gagtggttcg
     4261 aggccgcggc cgaggcttac accggcgagg ccaagaaggt cgtcaactgg atcacggcgg
     4321 acctcttcgg actattgaac gaagccgggc aggagcttca cgaatcgaag atcgcgccgg
     4381 ctcaactggc tcgactcgtt cagctggtcg acgacggaac cgtctctggg aaacaagcaa
     4441 agacggtctt gggcaagatg ttcgccaccg gttcagaccc ggccgatatc gccgccgagg
     4501 aagggttgag ccaggtttcc gacgcgaacc tgatcgaatc gttcgtcgaa gaggtgatcg
     4561 cggagaacgc ggaggcggcc gacaaggtcc gggcggggaa catggggacg atcgggttcc
     4621 tcgtcggaca ggtgatgaag aagacgaagg gccaggccaa ccctcagatg gtcaacgagc
     4681 tcctacgtag caagctctcc tcgtagtgag tctctagtgg tgacgatcga gatggccgag
     4741 accatgcccg gcccccccga agtcgtgtgg cgcttgatca ccgactggga gcaccaggac
     4801 gactggatgc tcgaggcctc tgatttcgtg gtcacgtccg atatccgcga gggcgtcggc
     4861 gtcgaagccg aagcgacgat cacgatcgcc gggatcaaga cgcgtgacaa ggtgagtgtc
     4921 gtggggtggg agccccagca ccgcctcgcg atccgacatc tcggttgggt ggcgggcgtg
     4981 ggcgagatct tcctcactcc gttggagggg ggccggacct acctgttctg gcgtgaggat
     5041 ctcgagccgc ccttgggtat cgtgggccgt gtcggcctcg gtctgctcaa gccgctgatg
     5101 agaaggatct tcgttcgcga cctgcgcatc cttgcgtccc taacccgcgc cgccacctcc
     5161 accgctctgc tctgacagca ggagccgccc gcgcggtggc gaaatcagga cgtatgagac
     5221 ggatcgtcgc gcttgctctt ggcactgtgc tcgccctgcc tttcgccgcc ggccttagtg
     5281 cgggggcggc cgcgggcgaa ccacctaaga ccggcttcga acaacgcaac ggggcctcgt
     5341 ggacgacaca
//
LOCUS       DATMED010000151         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3561293, whole genome shotgun sequence.
ACCESSION   DATMED010000151 DATMED010000000
VERSION     DATMED010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561293"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VNP73_03790"
     CDS             complement(<1..1363)
                     /locus_tag="VNP73_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65075.1"
                     /translation="MRKLFLVSMLAAFVTLIPSGFAHAHPLGNFSVNRFSGLEIGDEE
                     VTVHYVIDMAEVPTLQLLPVIDLDKDGGVDERELTRFVTSLGPKLGPLLKLQADGRSL
                     PLEVQRAEGRFSRGQGSLDVLRIEIDYSADLTSSSARIDYADGTFPGRRGWVEVVAYT
                     SGDQGIEDSSVPTTSVSSELRRYPKDMLKSPVAVQTAIVDVDPGAAAAPRPSDAAPQA
                     REVSRLEKAFTGLIERRMSPGVFVLAILIALAFGAVHALGPGHGKAIMAAYLVGTKGR
                     VRHAVTVGIAVSLMHTLSVIGLGAITLWASSMFPPEAVYPYLSLASAAVVLGLGGYLL
                     TKRLRSRHVHSHEHPHDHSHDHSHHHGHETVDVMSWKGLTAIALSGGLLPSPSALIVL
                     LGAVALHRVALGITLVGAFSIGLAGALAVIGILVLQARSVGVRFLGERTASAIPLISA
                     ALIF"
     gene            complement(1360..2670)
                     /locus_tag="VNP73_03795"
     CDS             complement(1360..2670)
                     /locus_tag="VNP73_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65076.1"
                     /translation="MRQGTRWTGVLTLALAMVVVGTLLPGRAERTDSPLAGTGAAVDS
                     LLAPTAGSDVPAVVARLQERVREDRNDVRSLALLGLAYLKRAGQTQDPVYLTKAGQVI
                     DRALALQPDGNYEAFVGAGNLALARHDFRRALMWGRRGAGLRPDAPEPLGITGDALIE
                     LGHYSAAAGAIQSMVDRRPALPSYARVSYFREMHGDLDGAVAAMRAAYSAAGTAVDAA
                     WVDVHLGELYIKQNRIDLATRHLRRAHATVPKDPAASFGLAHVAMIEGRRREAIRIMR
                     NVVLRYPSPVYAAFLGELFVAAGDRRSAGYQFDLVRAQIELAEANGGIADVETYLFLL
                     DHGSVTDDLVAELVALYTSRPSVRVADVYAWALFKSGRLHRAGRVIRAAVKLGTPDPL
                     IEFHAARIYSALGHYDRARAEMRKAVSHGLAAWPLQMKEARSRI"
BASE COUNT          543 a          955 c          893 g          463 t
ORIGIN      
        1 cgaagatcag cgcggccgat atcagtggga tcgccgacgc cgttcgttcc cccaggaacc
       61 ggacccccac cgaccgagct tgaagtacca ggatcccgat cactgccagg gctccggcca
      121 gcccgatgct gaaggcgcct acgagcgtga tccccagagc gacccgatga agagcgacgg
      181 ccccgaggag gacgatcagg gcactggggg aagggagcag acctcccgag agagcgatcg
      241 cggtcaagcc cttccacgac atgacgtcga ccgtctcgtg cccgtggtga tgagagtgat
      301 cgtgcgagtg atcgtgggga tgttcgtgcg aatgaacgtg ccggctcctc aaccgcttgg
      361 tcaagaggta tccccccagc ccgagaacca ccgcagccga tgccagtgac aggtacggat
      421 aaaccgcctc gggtgggaac atgctcgacg cccacagcgt gatcgcgccc agcccgatca
      481 ccgagagcgt atgcatcagc gacaccgcga tcccaaccgt gacggcgtgg cgtacgcggc
      541 ccttcgttcc cacgaggtaa gcagccatga tcgctttgcc atgcccgggt ccgagagcat
      601 gcactgcacc gaaagcaaga gcgatgagga tcgccagcac gaagaccccg ggcgacatgc
      661 gcctctcgat cagcccggtg aacgccttct ccagccggct tacctcgcga gcctgcggag
      721 cggcatcgga cggcctgggg gcagcagcgg ccccgggatc gacgtcgacg atcgcggtct
      781 ggaccgcgac gggcgactta agcatgtcct tggggtacct gcgtagttcg gagctgacgc
      841 tggtcgttgg aacggaagaa tcctcgatgc cctgatcgcc ggaggtatag gcgacgacct
      901 cgacccaacc ccggcgaccg gggaaggtgc cgtcggcata atcgatgcgc gcgctgctcg
      961 atgttaggtc ggcggagtaa tcgatctcga tccgcaagac atcgagactg ccctgccctc
     1021 gcgagaaccg cccctcagcg cgctgcacct ctagagggag tgatcgtcca tctgcctgca
     1081 acttcaggag ggggcccagt ttgggaccga gtgaggtcac gaaccgcgtg agctcccgct
     1141 cgtccacgcc gccgtcttta tcgagatcga tcaccggcag cagctggaga gttggtacct
     1201 ccgccatgtc gatcacgtag tgaaccgtca cttcctcgtc gccgatctcg agacccgaga
     1261 aacggttgac cgagaagttc cccagtggat gcgcgtgagc gaagcccgac gggatgagcg
     1321 tcacgaacgc tgcgagcatc gagaccagga agagcttcct catatccgtg accttgcttc
     1381 cttcatctgg agcggccatg cggccagacc gtgggatacg gctttccgca tctctgctcg
     1441 cgctcgatcg taatgcccga gcgccgaata gatccttgcc gcgtggaact cgatcagtgg
     1501 atcgggcgtc ccaagcttca cggcggctcg gatcactcgg cctgcgcggt gaagccggcc
     1561 gctcttgaag agtgcccacg cgtagacgtc cgccactctc acgctcggtc ggctcgtgta
     1621 cagggcaacc agctcggcca cgaggtcgtc cgtgaccgac ccgtgatcca acaggaacag
     1681 gtaagtctcc acatccgcta tcccgccgtt cgcctcggcc aactcgatct gagcgcggac
     1741 gaggtcgaac tggtaacccg cgctccggcg atcccctgct gcgacgaaca attcgcccag
     1801 gaaggctgcg tagacgggtg acggatacct gagcacgacg tttcgcatga tccggatcgc
     1861 ttcgcgtctc ctgccctcga tcatcgcgac atgggcaaga ccgaacgacg cggccggatc
     1921 cttcgggacg gtcgcgtgcg ccctccgtag gtgccgagtc gccaggtcga tccggttttg
     1981 cttgatgtag agctcaccca gatgaacgtc aacccaagct gcgtctaccg ccgtgcccgc
     2041 agccgagtaa gccgctcgca tcgcggcgac ggctccgtcc aggtcaccat gcatctcacg
     2101 gaaatacgac acgcgtgcgt acgagggcag agccggacgc cggtcgacca tgctttggat
     2161 cgcacccgca gcagccgaat agtgacccag ctcgatcaga gcatcgccgg tgatgcccag
     2221 cggctccggc gcatccggtc tcagtccggc gccgcgccgg ccccacatca atgcgcgccg
     2281 gaaatcgtga cgcgccagag cgaggttccc tgcacccacg aaggcctcgt aattgccatc
     2341 cggttgcagg gcgagtgcgc ggtcgatgac ctgcccggcc ttcgtgagat acacgggatc
     2401 ctgcgtctgc ccggcgcgct tcagataggc caagcccaga agcgccagcg accggacgtc
     2461 gtttcgatcc tcccgcacgc gttcctgcag tcgggcgacg accgcaggga cgtcacttcc
     2521 tgcggtaggt gccagcagag aatcgacagc agctcctgtg ccggcgaggg gcgagtcggt
     2581 ccgttcggcg cggccgggga gcaaggtgcc aacgacaacc atcgccagtg cgagggtcag
     2641 cacccccgtc catcgcgtcc cttgtctcat cgcaccgtcc atctctaaga ggtgaggagg
     2701 gcccgtgtgg gccctcctcc gctgataccg atcaaccgcc gtgcggtgcc gcgctcgaac
     2761 ccgagtgcgg gatcgctacg taagggaacg aagccgagaa ctcgacgtcg ttcacaccaa
     2821 cgccgtcgcc gaggtcgttc ggcgatccga cgag
//
LOCUS       DATMED010000152         4015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4333748, whole genome shotgun sequence.
ACCESSION   DATMED010000152 DATMED010000000
VERSION     DATMED010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4333748"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VNP73_03800"
     CDS             <1..341
                     /locus_tag="VNP73_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWL65077.1"
                     /translation="GQDNVVLVHYEDLSENLESEMRRLAGLLEIEVPDARWPKLVEAA
                     RFETMKARADQFAPDPSGILKSKRAFFRSGKRGAGQTILSDAELDRYRERTAQMASSD
                     LLAWLHRDPR"
     gene            448..852
                     /locus_tag="VNP73_03805"
     CDS             448..852
                     /locus_tag="VNP73_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65078.1"
                     /translation="MSSFRVLIAGALLTAALSVNSVAAADCSDPIFEMSESEVRPGDQ
                     VRISGHAWGDACNDTPGPGCNPPHLGQPIQDITFVLRNRTTGDSLEIATVDADENYGF
                     ELALTVPDIASGKYVVRDARKEAPGKRLRVLE"
     gene            1057..1188
                     /locus_tag="VNP73_03810"
     CDS             1057..1188
                     /locus_tag="VNP73_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65079.1"
                     /translation="MAHADVDEGNGKTFEFYCDEPPSLGGENQFPQPLTYIAAGVGF"
     gene            1219..1491
                     /locus_tag="VNP73_03815"
     CDS             1219..1491
                     /locus_tag="VNP73_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65080.1"
                     /translation="MLKKTIDRAECSVEFDWYLEGSVLRGTVEAGATECRTHFVIDSP
                     ESEQDILRIIRLAKRGCFAEQMVQSSVPLVSTFVVNGEELQVSLKE"
     gene            complement(2063..3835)
                     /locus_tag="VNP73_03820"
     CDS             complement(2063..3835)
                     /locus_tag="VNP73_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWL65081.1"
                     /translation="MRRLISVVALCALALTTQVLPVTAQALDDPIPRPINKGGPQVRL
                     EPVATGLTAPNWGISAPGDSNRLFVTDQDGILWAIDLASGAKTVFLDVSARLVPLGAF
                     GPGTFDERGLLGLAFHPDYANNGLLYTFTSEPVSGPADFSTMPPGTPANAQSVIAEWQ
                     VPNPRTAASVVDPSTRRELLRIDKPQFNHNAGALNFGPDGLLYISLGDGGNADDEGVG
                     HSPQGNAQDPSNVLGDILRIDPAGTNSNNGSYGIPADNPFVGEAGFVPEIWATGFRNP
                     FRFSFDSATGKLWAGDVGQNDIEEVDVVTEGGNFGWRVKEGSFLFDPNGDDPGFVTER
                     SPGEPEGLIDPVAEYDHDEGISVIGGFVYRGDAIPQLRGRYIFGDFARSFEEFGRLFH
                     FRSSGAGQIQEFRLVGQDSLGLKLDGFGQDADGELYVLGNTTGTPFGDTGVVMRIAPK
                     ERDNNLEADLNGFAEVDAATGQFGAGDLNGDGLSEIRLRSENERVCFSLRWADIDQPI
                     AAHIHSGVVGVNGPIVVDLLSNADNFRHENGEGRANGCAEDVDPELIDDIGNHPSAYY
                     VNIHTSPFPGGAIRGNLERTEVGI"
BASE COUNT          751 a         1181 c         1310 g          773 t
ORIGIN      
        1 ggggtcagga caacgtggtt ctggtccact acgaagacct ctccgagaac ctcgagagcg
       61 agatgcggcg acttgccgga ctcctcgaga tcgaagtgcc agacgcgagg tggccgaaac
      121 tcgtggaggc cgcgcgcttc gagacaatga aggcgcgcgc ggaccagttc gcaccggacc
      181 cgtcggggat cctcaagagc aagcgagcgt tctttaggtc gggaaaacga ggagcgggtc
      241 aaaccattct ctccgatgcg gagctggatc gctaccgcga acggacggcg cagatggcgt
      301 cctccgacct gctggcttgg ttacatcgcg accctcggta gggtgagcca acatctgacc
      361 gagaccctga gccgtacggg gaaccttgtt cgggcgcacg gcgtctgaga cattggaagg
      421 gtaggtcggc cggggggatg gaagatcgtg agcagttttc gggttctgat tgcgggggcg
      481 ctcttgactg ccgctctctc ggtcaattcg gtggccgcgg ctgattgttc ggatccgatc
      541 ttcgaaatga gtgagagcga ggtcaggccg ggcgatcaag ttcgcatcag tggccacgct
      601 tggggggacg cctgcaatga caccccggga cccggctgta accctcctca tctcggacag
      661 ccgatacagg acatcacctt cgtccttcga aataggacca cgggcgattc attggagatc
      721 gccacggtgg atgcggacga gaactatggg ttcgaactag cgcttacggt gcccgacatc
      781 gcctcgggaa agtacgtcgt ccgggacgcc cgaaaagagg cgccaggcaa gcggctcagg
      841 gtgcttgagt aggtgctgga tgggtgctct gctcgctctc gcgcactctc tgtacagtgt
      901 cggtttcgat catcgttcga gggagaagag atgccatctt cagagcgacc agttgtcggt
      961 caatcgactc ctgccccctc gccggaacac gcaccggaga agggacgaag gtttccgaag
     1021 tctgctcgca tcagaatcat caaggatcag ctcaaggtgg cacatgcgga tgtggatgaa
     1081 ggcaacggta agacgttcga gttctattgc gacgaaccgc cttcgcttgg aggcgagaac
     1141 cagtttcctc agcccctcac ctacattgcc gcaggggtcg gcttctgact gcttactcaa
     1201 gtgacgcggt acgcgtcaat gctaaagaag accatcgaca gggcagagtg ctccgtcgag
     1261 ttcgactggt acttagaggg atcggttctg cgaggcacgg tcgaggcggg ggctactgag
     1321 tgtcggactc acttcgtcat tgactcgccg gagtcagagc aggacatcct tcggatcatc
     1381 cggcttgcca agcggggatg cttcgccgag cagatggtgc aatcgtccgt cccgttggtc
     1441 agtacgttcg tggttaacgg cgaagaactc caggtgtcgc tgaaggagtg atggagacga
     1501 aaggaaacag ggggggcgca ccgaactggc gcgcaccctt aacccaaggg gagcctgatg
     1561 ggtgtcagcg gcggcccact ggtggagtgg atctcggtgt cgtaatcatc gctggcggcg
     1621 gctggaacag tgggttcaga ccaatcgtcc gacgtgcatg gccacagccg agatcggtca
     1681 ggagaggacg aggagaaacc atgaggcgtg ccacttcgat actagctgga gcatcgttgc
     1741 cgccttgctc ttcccagctg gcggaggcag tgagtgtggc tatacgacca ccgggggagt
     1801 gtgtgagtcg tggtccgata gcggcctcgt ccgctacagc ggcgagaacg gagcggtcgg
     1861 tatgggcatc gccctaggcg acgggtctcg cctcggccat cctggtctac cttctgattc
     1921 ggctgctttc cgcccgcagc ggctatcaac gaggcctcag agcaccctgc cgacttgctg
     1981 agaactatga agcgtcgtct gatccgtcac ggacgagggc ggattgaacg gttgggtaag
     2041 cgtttgtttg aataaagagc gtttagatgc cgacctcggt tcgctccagg tttcctctaa
     2101 tggctcctcc agggaagggc gacgtgtgga tgttcacgta gtacgcgctc gggtggttac
     2161 caatgtcgtc gatcagctcc ggatccacgt cctcggcgca gccgtttgcc ctgccctcac
     2221 cgttctcgtg acggaagttg tccgcgttgg ataacaggtc aacaacaatg ggcccgttta
     2281 ccccaaccac gcctgagtga atgtgcgccg caatcggttg gtcgatgtcg gcccaccgga
     2341 gactgaagca gactcgctcg ttttcggacc gcagacggat ctcagagagg ccgtcaccgt
     2401 tgaggtctcc tgccccgaac tgaccggtgg ccgcgtcgac ctccgcgaac ccgttcaggt
     2461 ccgcctccag attgttgtcg cgctccttgg gagcgatcct catcacaact ccggtgtcgc
     2521 caaacggcgt gcccgtcgtg ttgccgagca cgtacagctc gccgtcggca tcctggccga
     2581 acccatcgag cttcaagccg agggagtcct gtcccacgag cctgaactcc tgtatctgac
     2641 cggcccccga agaacggaag tgaaacagcc gcccgaattc ctcgaagctg cgggcgaagt
     2701 ccccgaagat gtagcgcccc cggagttgcg gtatggcatc accgcggtag acgaaccctc
     2761 cgatgaccga gatgccctcg tcgtggtcgt attccgcgac cggatcgatg agaccctcgg
     2821 gctctccggg gctcctttcg gtgacgaagc cgggatcgtc gccgttcggg tcgaacagga
     2881 acgagccttc cttgaccctc cagccgaagt tcccgccctc tgtcaccacg tcgacttcct
     2941 cgatgtcgtt ctgtccgacg tcgccggccc acagcttgcc ggtagccgag tcgaacgaga
     3001 accggaatgg gttgcggaaa cccgtggccc agatctcggg aacgaagcct gcctcaccga
     3061 cgaacgggtt gtcggcggga attccgtagc ttccgttgtt cgaattcgta ccggccgggt
     3121 cgattcgcag gatgtcgccg agcacgttgc tcgggtcctg ggcgttgcct tgagggctgt
     3181 ggccgacgcc ctcgtcgtcc gcgttgccgc cgtctcccaa ggagatatac agaagaccgt
     3241 cgggtccgaa gttaagcgcg ccggcgttgt ggttgaactg gggcttgtcg atcctgagaa
     3301 gctcgcgccg cgtgctcgga tccacgaccg aggccgccgt cctcgggttg ggcacctgcc
     3361 actccgcgat cacgctctgg gcgtttgccg gggtgcccgg aggcatcgtc gaaaagtcgg
     3421 cgggaccgct gacgggctct gacgtgaagg tgtagagcag gccgttgttc gcgtagtcgg
     3481 ggtggaaggc gagtcccagc aggccgcgct catcgaaggt gcccgggccg aacgcgccca
     3541 gtggcaccaa acgggcggag acgtcgagaa atacggtctt ggcgcccgaa gcgaggtcga
     3601 tggcccacag gatgccgtcc tggtcggtta cgaacaggcg gttggagtcg cccggagcag
     3661 agatgcccca gttgggggcc gtgagtccgg tggccacggg ctcgagccgg acctgagggc
     3721 cgcccttgtt gatgggccgc gggatcggat cgtccagcgc ctgcgcggtg actggcaaaa
     3781 cttgggtcgt gagcgctaag gcgcacaacg cgacaactga gatgagtctt ctcatctgga
     3841 gcccccctta ctgttggtcg gacctgaagc gcacacgacg ccggtgacca cccctcagct
     3901 cccccttaag ccggagcgcc gctatcccgg tatacggctg agggacgttt gggttctatc
     3961 caaggccaag aaaaaggtat tcgcgtccat gcgctgtgct tacctgcatt tcgca
//
LOCUS       DATMED010000153         3267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2047053, whole genome shotgun sequence.
ACCESSION   DATMED010000153 DATMED010000000
VERSION     DATMED010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047053"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /gene="thpR"
                     /locus_tag="VNP73_03825"
     CDS             complement(<1..486)
                     /gene="thpR"
                     /locus_tag="VNP73_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWL65082.1"
                     /translation="MTDKIRTFIAVDIPEGLLQTLDAAIVALKSRWPHARWAPVGNQH
                     VTLRFLGPTPVDQLPAITETVAGIAAEHAAAELSLDELGSFPSKRRMRVLWVGLDDPT
                     GLLAALAGCLSDRLAPLGFEAEERGFSAHLTVARFKQPERLDEPLPDLDLSDRAFLVS
                     DL"
     gene            complement(483..1718)
                     /locus_tag="VNP73_03830"
     CDS             complement(483..1718)
                     /locus_tag="VNP73_03830"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HWL65083.1"
                     /translation="MRAEIVGVGTELLLGQIANTNARVISRALAAIGIDVYFHTVVGD
                     NPDRMIDTIRRAMERADAVVVTGGLGPTPDDITREAVAAAVGVALIRDEELVSYIKGV
                     FEKLGRDMPENNLRQADLPEGATPIVPEGTAPGFSLESAGKIVFALPGVPWEMQAMLQ
                     KSVIPKLKELGGDQTILSREVLVVGLGESKAHELIHDIVEAQSNPTIAYRASAAQVRL
                     RITAKASSESEALGLIDPVEKQIRERVGEDAVSGRHASLADALGEMLRERGATMAAAE
                     SLTGGLIAAEITKTSGAGDYFLGSIVSYHTAAKRDLVGVDEGILNGPGAISEAAAAAL
                     AEGVAQRFSADLGISATGVAGPTEQEGKPVGTIFVGATFGGKTEVRQVRGYGDRAHVQ
                     GIAVTSALDLGRRLVLRSG"
     gene            complement(1715..2239)
                     /gene="pgsA"
                     /locus_tag="VNP73_03835"
     CDS             complement(1715..2239)
                     /gene="pgsA"
                     /locus_tag="VNP73_03835"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWL65084.1"
                     /translation="MNLPNVITIIRLFLVPVFVYFAFKGTDTGRLIALVTFVVASLSD
                     IVDGYIARKRGLVTRLGEFLDPLADKILVLAALVFLIENRTFPVIVALVIFFREIAVQ
                     ILRNRIVARGGTLPASPAGKIKTVVQIVMVSWWLAFTPVGVGHWVLLAGALAATLWSG
                     AEYFLRFNRLQEVS"
     gene            complement(2285..>3267)
                     /locus_tag="VNP73_03840"
     CDS             complement(2285..>3267)
                     /locus_tag="VNP73_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65085.1"
                     /translation="NNRFYLREEKSVEAPIDPQDPRNPLYVGTSRYLVDEIETLHEAV
                     HRSFGDWDPQDGATSSEAYTASVDEPSARGADYATLEPDQSFPNPADYIATQNPSELA
                     QDTTSSCAEEDPDGYGLESTLDHQGQTQARRLFEDTAQHPLPKYLINNFTITDGSGHH
                     FGPHTTCQIAAYRDSDLRLGRILTAMRSSGALGESLIVITGDHGSENQNLKNKGLPSD
                     FEARLNEANIAHVMADWHVYLRTVDVATSVGKLTRGANEVTFTVTDDDTGEAVLGATV
                     AIKGATEPAEGTTDAAGKTSLDFTPAGRRVTVVVSAGGFNKRLLRLKVSP"
BASE COUNT          585 a         1071 c         1016 g          595 t
ORIGIN      
        1 caggtcggag accagaaaag cccggtctga gaggtccagg tcgggcagcg gctcgtccag
       61 tcgttcgggt tgcttgaacc gggccacggt taggtgggcc gagaaccccc tctcttcggc
      121 ttcgaagccc aggggggcga ggcggtcgga caggcagccg gccagggctg caagaagccc
      181 ggtcgggtcg tccaggccca cccacaggac gcgcatccgt cgcttagaag ggaacgagcc
      241 gagctcgtcg agcgataact cggccgcagc gtgctcggcg gcgatccccg caacggtctc
      301 ggttatcgca gggagttggt cgaccggggt cggccccagg aatctgaggg tcacatgctg
      361 gttcccgacc ggagcccagc gagcgtgggg ccagcgggac tttagagcga cgatggcggc
      421 atctagcgtc tgcagaagcc cttcggggat atcgaccgcg atgaacgttc ggatcttgtc
      481 ggtcatccgc tccgaaggac gagacggcgg ccgagatcga gcgcggacgt caccgcgatc
      541 ccctgcacgt gggctctgtc gccgtaaccg cgcacctgcc tcacctcggt cttacctccg
      601 aatgtcgccc ccacgaagat cgtgccgacc ggcttgccct cttgctcggt cggaccggcc
      661 acccccgtcg ccgagatccc gagatcggcc gagaaacgtt gcgcgacgcc ttccgcaagt
      721 gccgccgcgg cggcctcgct gatcgcaccc ggaccgttca ggatcccctc gtcgactccg
      781 accaggtcgc gcttcgctgc cgtgtggtag ctcacgatcg agccgaggaa gtaatcgccg
      841 gccccgcttg tcttcgtgat ctcggcggcg atcaaccctc cggtgagcga ctcggcggcc
      901 gccatcgtcg cccctcgttc cctcagcatt tcgccgagag catcggccaa gcttgcatgc
      961 cggccactca ccgcgtcctc gcccacccgc tcacggatct gcttctccac cgggtctatg
     1021 aggccgagcg cctcgctctc ggacgaggcc tttgccgtta tccggagccg aacctgtgcc
     1081 gccgacgccc gataggcgat cgtcgggttg gattgggcct cgacgatgtc gtgtatcaac
     1141 tcgtgcgcct tagattcgcc gaggccgacg accagcacct ctcggctcag gatcgtttgg
     1201 tcgcccccga gttccttcag cttcgggatc accgatttct ggagcatcgc ctgcatctcc
     1261 cacggcacac ccggcagtgc gaacacgatc tttccggccg actctagaga gaagccggga
     1321 gcggtgcctt ccgggacgat cggtgtcgcc ccttcgggca ggtctgcttg gcggaggttg
     1381 ttctcgggca tgtcgcgtcc gagcttctcg aacacaccct tgatgtaact aacgagttcc
     1441 tcatctcgga tcaatgcaac accgacggca gcagcgaccg cctcccgcgt gatgtcgtcc
     1501 ggggtgggcc ccaacccgcc cgtgaccacc accgcgtcgg ctcgttccat cgcccgacgg
     1561 atcgtgtcga tcatgcggtc cgggttgtcc ccgacgacgg tatgaaagta gacgtcgatc
     1621 ccgatcgcgg cgagtgccct cgagatcacg cgggcattcg tgttcgcgat ctgaccgagg
     1681 agaagctcag tccccacccc gacgatctcg gctctcatga cacctcctgc aagcggttga
     1741 agcgaaggaa gtattcggcc cccgaccaca gcgtggccgc caacgcgccg gcaagaagta
     1801 cccagtgacc gaccccgacc ggggtgaagg cgagccacca cgacaccatc acgatctgca
     1861 ccacagtctt gatcttcccc gcaggagacg cgggaagcgt cccgccgcga gccacgatcc
     1921 gattacgcag tatctgcacg gcgatctcac ggaagaagat cactaacgcc acgatcaccg
     1981 ggaaggttcg gttctcgata aggaacacga gggcagccag taccaggatc ttgtccgcga
     2041 ggggatccag gaactccccc agcctggtga ccagcccccg cttccgagcg atgtagccgt
     2101 cgactatgtc cgacagcgag gcgaccacga acgtgacgag agcaataagg cggcccgtgt
     2161 cggtgccctt gaacgcgaag tacacgaata cgggaaccag gaacaagcgg atgatggtga
     2221 tgacgttggg aagattcacg gggggcaggt taaagccgcc gcagtgcaac gacctatggg
     2281 aacctcaagg gctcactttg aggcgcagaa gcctcttgtt gaagccaccc gcggatacga
     2341 caacggtcac acgccggccg gccggtgtga agtcaagcga ggtctttccg gcggcatcgg
     2401 tggtgccctc cgccggctcg gttgctcctt tgatcgccac ggtggctccc agaaccgcct
     2461 cgcccgtgtc gtcgtcggta acggtgaacg ttacttcgtt cgctcctcta gtcagctttc
     2521 ccacgctcgt cgccacgtcg acggtgcgca ggtagacatg ccagtccgcc atcacgtgtg
     2581 cgatgtttgc ctcgttcaaa cgcgcctcga agtctgaggg gagccccttg ttcttgaggt
     2641 tctgattctc ggatccgtga tcgcccgtga tgacaatcag cgactcaccc aaagcgcccg
     2701 agctgcgcat ggccgtgagg atccggccga gacgcagatc agaatctcgg tatgcagcga
     2761 tctgacaggt cgtgtgcgga ccgaagtggt ggccggaacc atcagtgatc gtgaagttgt
     2821 tgatcaagta cttcggcaag gggtgttgag cggtgtcctc gaaaagccgg cgcgcctgtg
     2881 tctgaccctg gtggtccaag gtcgactcca agccatagcc gtcgggatcc tcctccgcgc
     2941 acgagctcgt cgtgtcctgt gctagctcgg acgggttctg ggtcgcgatg taatccgccg
     3001 ggttcgggaa actctgatcg ggctcgaggg tcgcgtagtc cgcgccgcgc gcgctcggct
     3061 cgtcgaccga agccgtgtag gcctccgacg aggtcgcgcc gtcctgtgga tcccagtccc
     3121 cgaacgacct atgtactgct tcgtgaagag tttcgatctc atcgactaga taccgggacg
     3181 taccgacgta gagcggattg cgaggatcct gaggatcgat cggcgcctcc acggacttct
     3241 cctcacgcag gtagaagcga ttgttca
//
LOCUS       DATMED010000154         3294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1653481, whole genome shotgun sequence.
ACCESSION   DATMED010000154 DATMED010000000
VERSION     DATMED010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653481"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VNP73_03845"
     CDS             <1..1000
                     /locus_tag="VNP73_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWL65086.1"
                     /translation="HCLLALTSEVWLKTISLESQMHLFTDLVPFLLHDYAQQGEDLPA
                     IPELFVPLLEGLEPRPQPEVFMTYFDHVSPEDPELPAYRVNFLVIRLAGPDGAFGGIV
                     CFGYMAIRPNLVALLARGDEAMYQRMAKLTEPGSRQAAILFCDIAGSGDLSRRMSSAT
                     YFKLVRELWTSTDAAVAANRGMIGKHAGDGASAFFLVDDLGSASGAARAALATARRVH
                     ELAGEIFQSAIDSSCLMRVGVHWGGTLYMGQLVPGGRLDVSALGDEVNECARIQEVAT
                     RDQTLASKQLLEQLTDDDAASLGLDLEKIQYSLVSELPDAPEKVVRDAGAISVASV"
     gene            complement(997..2157)
                     /locus_tag="VNP73_03850"
     CDS             complement(997..2157)
                     /locus_tag="VNP73_03850"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWL65087.1"
                     /translation="MPEAVIAAAARTPIGRAKKGSLLDVRPDDLMAFALKSVMEKAPE
                     VDAGEIVDVMIGCGFPEQKQGMNLGRRAALLAGFPDHVPGTTVNRFCASSLQTIRMAF
                     HAIKAGEGDVFVAGGVESISQVDGYPKDVDELHPKLFGDDAEIANVYIPMGNTAENVA
                     EQYDVSREDMDKFAQQSQERAVAAQENGFFARELTPYTKEDGTVVDKDDGPRPSSTLE
                     KLQSLPSAFKEDGKVTAGNSCPLNDGAAAVLVMSQERADQLGVKPLARIIASSVSGLA
                     PEIMGVGPIEAVRKVLKQAGMSIDDIETVELNEAFAAQVIAVVREVGVDPFSDKLNPN
                     GGAIALGHPYGMTGARIMNTLLNDLATRDQTIGLETMCVGGGQGMAMIIERL"
     gene            complement(2197..>3294)
                     /locus_tag="VNP73_03855"
     CDS             complement(2197..>3294)
                     /locus_tag="VNP73_03855"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HWL65088.1"
                     /translation="VNDATTKQGQDYSTTAGEYRQGCFACHGIHYFITGDTDNAMSPA
                     KTTEIDGQQWQWAAPTEQPMNVGDRYANTVKAPLVASVVTTPNTPQDATVTTVAITGA
                     TGGTIGETVTLAGRLTDGETGIAGKELAFSLRGEPVGSAITDENGDAAVQTTLTGPAE
                     ATQQTVTFEGEGQFTPSSVTENFEVARVATELALTGEASGQTSDKATVSARLTSNGNG
                     LSGREVVFTLGDEEVGRATTDESGGAAVQTTLSGAARTVDLSASFAGDGGHDPASATG
                     PFEIRRDDSVLRSTSSLRAGRVTATATLTDADSGAGLEGRTIRFLANGVEVATAVTDA
                     QGVATTTFKGKKSTAVTASFDGDDSYNASQS"
BASE COUNT          521 a         1076 c         1043 g          654 t
ORIGIN      
        1 gcactgcttg ctcgcactga cgagtgaagt ttggcttaag acgatctccc tcgaaagcca
       61 gatgcacctc ttcaccgacc ttgtcccgtt cctccttcat gactacgccc agcagggaga
      121 ggatctcccc gcgatccccg aactattcgt ccctctcctc gagggactcg agccgcgccc
      181 gcaacccgag gtcttcatga cgtacttcga ccacgtttcc ccggaggacc cggagctccc
      241 cgcatatcga gtgaatttcc tggtcataag actcgccggt cccgatggag ccttcggagg
      301 catcgtgtgc ttcggctata tggccatcag acccaacctc gttgcactgt tggcgcgggg
      361 tgacgaggcg atgtatcagc gcatggcgaa gctcaccgaa cccggctccc gccaagccgc
      421 gatcctgttc tgcgacatcg cagggtcggg agatctgtca cgccggatgt cgagcgccac
      481 ctacttcaag ctcgtgcgtg agttgtggac gtcgaccgat gccgccgtag ccgccaaccg
      541 ggggatgatc ggcaagcatg ccggcgacgg ggcgtcggcc ttctttctgg ttgacgacct
      601 cggatctgct tcgggtgcgg ctcgtgcggc cctagctacg gctcgacggg tgcacgaact
      661 tgcgggagag atcttccaga gcgctatcga ctcctcgtgc ttgatgcggg tcggggttca
      721 ctggggtgga accctctaca tgggccagct ggttcccggc ggccgcctcg acgtttccgc
      781 tcttggggac gaggtcaacg aatgtgcacg catccaagag gttgcgaccc gcgaccagac
      841 gctcgcgtcc aagcaactgc tcgagcagtt gaccgacgac gacgcggcga gcctcggcct
      901 ggacctggaa aagatccagt actcgctggt gagcgagctg cccgacgctc cggagaaggt
      961 tgtccgggac gcgggcgcaa tctccgtagc ttctgtctag agcctctcga tgatcatcgc
     1021 catgccctgg cccccaccga cgcacatcgt ctcgagaccg atcgtctgat cgcgcgtggc
     1081 caggtcgtta agcagcgtgt tcattatgcg cgcgcccgtc atcccatagg ggtggccgag
     1141 agcgatcgcg ccgccgttcg ggttgagctt gtctgagaac gggtcgacac cgacctcacg
     1201 gacgactgcg atcacctgcg cggcgaaggc ctcgttcaac tcgacggtct cgatgtcgtc
     1261 gatcgacatg ccggcctgct tgagcacctt gcgaacggcc tcgatcggac cgacacccat
     1321 gatctcgggc gcgaggcccg acacggacga agcgatgatc cgggcgagag gcttgacgcc
     1381 gagctgatcg gcccgctctt gactcatcac cagaactgcg gcggcgccgt cgttcaaggg
     1441 gcacgagttg ccggctgtca ccttgccgtc ttccttgaac gccgacggca gtgactgcaa
     1501 cttctcgagc gtcgagctgg gccgcggccc gtcgtccttg tcgacgaccg ttccgtcttc
     1561 cttcgtgtaa ggggtgagct cacgagcgaa gaagccgttc tcctgtgccg ccacggcacg
     1621 ctcctgtgac tgctgcgcga acttgtccat gtcttcgcgc gacacgtcgt actgctcggc
     1681 gacgttctcc gccgtgttgc ccatcgggat gtagacgttc gcgatctcgg cgtcatctcc
     1741 gaacaacttc gggtgcaact cgtccacgtc cttgggataa ccgtcgacct gcgagatcga
     1801 ctcgaccccg ccggcaacga aaacatcgcc ctctccggct ttgatcgcgt ggaacgccat
     1861 ccggatcgtc tgcaggctgg acgcgcagaa acggttcacg gtcgtgcccg gcacatggtc
     1921 ggggaatccc gcgaggagag cggcccggcg gccgaggttc atcccttgct tctgctcggg
     1981 gaaaccgcag ccgatcatca cgtcgacgat ctcgccggca tcaacctcgg gggccttctc
     2041 catcacggac ttcagggcga acgccatcaa gtcgtcggga cggacgtcga gtaacgatcc
     2101 cttcttggcc ctgccgatgg gcgtacgagc ggcagcggcg atgacggctt cgggcatcag
     2161 gagctccttt cggcgtggac cgccgagcct aatgccctag gactgcgatg cgttgtaaga
     2221 gtcgtctccg tcgaatgagg cagtcacggc cgtgctcttc ttgcccttga aggtcgtcgt
     2281 tgcaacgccc tgtgcatcgg tgacggcggt ggcaacctcg accccattcg cgaggaagcg
     2341 gatcgttcgg ccctcgagac ctgcacccga atcggcatcc gtcagggttg cggttgctgt
     2401 gacgcgaccg gccctcaacg agctggtcga tctcagcacc gaatcgtccc ggcggatctc
     2461 gaacggtccc gtagctgagg ccggatcgtg gccgccgtca ccggcgaagg aggccgatag
     2521 gtcgactgtc cgggcggctc cgctcaaggt cgtctggacg gcggcgccgc cgctctcatc
     2581 cgtcgtcgcg cggccaactt cctcatcgcc gagcgtgaac acgacttccc gtccactaag
     2641 tccgttgccg ttggaggtca gtctggcgct cacggttgcc ttgtcggatg tctggcccga
     2701 ggcctcgccg gtcagcgcaa gctcggtggc cacacgagcg acctcgaagt tctcggtgac
     2761 cgagctgggc gtgaattggc cctcgccctc gaacgtcacc gtctgctgag tcgcttcggc
     2821 gggaccagta agtgtcgtct ggacggcggc gtcgccgttc tcgtcggtga tggcacttcc
     2881 aacgggctcg ccgcgcagtg agaaggcgag ttccttaccg gcgatgccgg tctcgccgtc
     2941 ggtcaggcgg cctgccaggg tgaccgtctc gccgatcgtt ccgcccgtcg ctccggtgat
     3001 cgccacggta gtgacggttg catcctgcgg agtgttgggg gtcgtgacga cgctggcgac
     3061 gagtggagcc ttgaccgtgt tggcgtagcg gtctccgacg ttcatcggtt gctcggtcgg
     3121 agcggcccac tgccactgct ggccgtcgat ctcggtcgtc ttagccgggc tcatcgcgtt
     3181 gtcggtgtcg ccggtgatga agtagtggat cccgtggcaa gcgaagcagc cctgccggta
     3241 ttcgccggcc gtggtgctgt aatcctgacc ctgcttcgtc gtggcgtcat tgac
//
LOCUS       DATMED010000155         4672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2049262, whole genome shotgun sequence.
ACCESSION   DATMED010000155 DATMED010000000
VERSION     DATMED010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049262"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /gene="ccsA"
                     /locus_tag="VNP73_03860"
     CDS             complement(<1..552)
                     /gene="ccsA"
                     /locus_tag="VNP73_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWL65089.1"
                     /translation="MDAASLGGPALVVALIFATGAAGLCIFSALRDRPVLAEMGRRAL
                     AIASAFVVFAALLLLQAFVTHDFSVAYVANYSSRSLPGAYTMAALWGGMEGSLLFWTV
                     LLTVFSWIALRQARRWADETLSAWSTAVLGGIVIFFLILLVGPADPFVRLSEIPADGQ
                     GLNPLLQSPGMLAHPPLLYTGFVG"
     gene            complement(560..976)
                     /locus_tag="VNP73_03865"
     CDS             complement(560..976)
                     /locus_tag="VNP73_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWL65090.1"
                     /translation="MDPSSTVPSTKRRAKFVVGALLIVVALAVMVGWAMSRPGSTAFY
                     ITPTELLAMGETDPTSDYRINGKVVPGSIERDGLRTRFVVTDGEAQVNVTTRAPIPDT
                     FRDRSEIVAKGYFDGSRFAAREVLAKCPSKFKAKQA"
     gene            1024..1578
                     /locus_tag="VNP73_03870"
     CDS             1024..1578
                     /locus_tag="VNP73_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWL65091.1"
                     /translation="MKARTRGLAAGVVAILVVLSVPGPVAAHGEFESAQPEPDSEVGR
                     PPRTVAITLTEPPSPGTVVAVTDGCSRDLVTDIQNSDDVVRAALKKGQPGKWTVRFRA
                     VSAVDGHTSRDSFAFKVTGKKDCSKPDRKEKSPDVQVEGGEDTQISSDDFPEDEGSSF
                     PIVPFAIGSVVVIAGAVLLRRATK"
     gene            1596..1946
                     /locus_tag="VNP73_03875"
     CDS             1596..1946
                     /locus_tag="VNP73_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65092.1"
                     /translation="MALVLLGACSSDDTPAPGGSSVRDAVAALCDARDTARDSVGEAR
                     DIFFNESHDPLHELARTATDEEPGAAAGLLEAKNAVEAAFEEGDDVEPELGTLISAAN
                     EALTAVSEEPVTCS"
     gene            1937..2899
                     /locus_tag="VNP73_03880"
     CDS             1937..2899
                     /locus_tag="VNP73_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65093.1"
                     /translation="MFIRAARIALAAVIATMLIACGGNEPEPQGGPSGPSGELIVQVA
                     SYDIAVGDEGRVILGLLTPAQLFVSYGTVDVEFFYLGTEEGSGKAQPGPTTTGTFLVI
                     PGDTEPEGAAGPIAAPASRGRGVYATEVAFDRAGFWSAQVTADVEDEGRLTGNAVFEV
                     LKEHAIPAPGDPALKTDTLTIDSKDAPEAAVDSRAAQGDIPDPELHQMTIAETIQRGE
                     PALVVFATPVYCVSRFCGPITDMVADLAKKYDDRANFIHVEIWRNFEGQVVNKGAADW
                     LLRGQDLSEPWVFLIGADGKVTARWDNVATPEEIEPLLKKLPRL"
     gene            2896..3516
                     /locus_tag="VNP73_03885"
     CDS             2896..3516
                     /locus_tag="VNP73_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWL65094.1"
                     /translation="MTVAPARVHDLTRGLKLEYVSLGYNLIETGVGITAGVAAGSVAL
                     IGFGLDSLVESASALILVWRFKTEARGARTSEEAERRAVKLVAVAFFALAAYVGINAV
                     FELIRGIRPESSPVGMGLAVASLIVMPWLAHRKRKLADEMGSASLRADSRQTTLCTYL
                     SGVLLFGLVANSLLGWWWADPVAAAAIAVLALTEGVELWRTEDFCC"
     gene            complement(3518..4111)
                     /locus_tag="VNP73_03890"
     CDS             complement(3518..4111)
                     /locus_tag="VNP73_03890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWL65095.1"
                     /translation="MVPTNELEGFRHRSRARKIVVLAAIACVLAVVVVGFLRPAKDGG
                     AGGAVPDFELPLVNESGELSSADLKGDPVVVNFFASWCLPCREEAPLLQRTYEKYQDR
                     GVRFVGVAIRDAASDTKDFVERYGITYPVIHDPAEAFAGPVGVLGLPETYFVDRNWTF
                     AGESSQDEVANRQGTVWFGPIDATELERSIEAMLESR"
     gene            4161..>4672
                     /locus_tag="VNP73_03895"
     CDS             4161..>4672
                     /locus_tag="VNP73_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWL65096.1"
                     /translation="MKRPSSARRGIATLSSFALVLLAACADNPPQDFLNPEGPVAREA
                     DKLWDITFLIAAVIFVIVQGLLVFAIFRYRARPGVEARQFHGNTKVEVILTVIPALIL
                     AGLAVPTVKTIMTLSEKPTGNVLEIKVIARQFWWEYQYSDLGFVTANEMHVPEDTPVY
                     LTLEGDDVIH"
BASE COUNT          854 a         1471 c         1505 g          842 t
ORIGIN      
        1 cccgacgaaa ccggtgtaca ggagcggggg gtgagcgagc atcccgggcg actgcaacag
       61 tgggttgagc ccttgcccat ccgcggggat ctcactgagc ctcacgaacg ggtctgcagg
      121 gccgaccaga aggatcagga agaagatcac gatgccgccg agcaccgcgg tcgaccatgc
      181 cgacagcgtc tcgtccgccc accgcctcgc ttgccgcaat gcgatccacg agaacacggt
      241 taacaagacg gtccagaaga gcagcgaccc ttccatgccc ccccacaacg cagccatcgt
      301 gtaggccccg ggtagcgatc gggacgagta gttcgcgaca tacgcgacgg agaagtcgtg
      361 cgtcacgaat gcttgcaaaa gaagtagcgc cgcgaacacc acgaacgcgc tcgcgatcgc
      421 cagagcccga cgacccatct cggccaacac gggtcgatct ctcaacgcgg agaagatgca
      481 gagcccggcg gcaccggtag cgaagatcag ggcgaccact agcgccgggc cgcccagcga
      541 ggctgcgtcc atcgctacct caggcttgct tggccttgaa cttagacggg catttcgcca
      601 gtacctcgcg agcggcgaaa cgggatccgt cgaagtagcc tttggccacg atctcggaac
      661 gatcccggaa cgtgtccggg atcggggccc ttgtggtgac gttgacctga gcctcgccgt
      721 cggtcacgac gaagcgcgtt ctcaggccgt ctctctcgat cgaaccggga accaccttcc
      781 cgttgatcct gtaatcgctg gtgggatcgg tctcgcccat cgccaacagc tctgtggggg
      841 tgatgtagaa cgctgtggaa ccgggacggg acatcgccca gccgaccatg acggccaggg
      901 cgaccacgat gagcagagcg ccgactacga atttggctct gcgctttgtg gaggggacgg
      961 tagaagatgg gtccatcgtt tcgatagaca ctaaatgaac ctgacaggga cggagtgtgg
     1021 gttgtgaaag ccagaactag gggtctcgca gcgggtgtgg tcgcgattct cgtcgttcta
     1081 tccgtcccgg gacccgtggc cgctcacggc gagttcgaat ccgctcagcc cgaacccgat
     1141 tccgaggtgg gacggcctcc acggacggtc gcgatcactc tgaccgagcc tccttcgccc
     1201 ggcaccgtgg tggccgtgac cgacggttgc agccgggacc ttgtcacgga catccagaac
     1261 tccgacgatg tcgttcgagc cgccctgaag aagggtcaac cgggcaagtg gacggtgagg
     1321 ttccgggccg tctcggcggt cgacggtcac acgagccgcg attcgttcgc tttcaaggtg
     1381 acgggcaaga aggactgctc gaagccggac cgcaaggaga agtcgcccga cgtccaggtc
     1441 gagggcggcg aagacacgca gatctcgtct gacgacttcc ccgaagacga gggttcgagc
     1501 tttccgatcg ttccgttcgc gatcggatcc gtggtcgtga tcgccggggc cgttctcctg
     1561 cgtcgcgcga cgaagtgatc ctgaggacgg cactggtggc tcttgtcttg ctcggcgcgt
     1621 gctcgtcgga tgacaccccc gcaccgggtg gatcctccgt gcgcgatgcc gtagcggctc
     1681 tctgcgatgc tcgcgataca gcgcgagact cggtcgggga ggcgcgggac atctttttca
     1741 acgagtccca cgacccactc cacgagctcg cacggacggc gaccgacgag gaaccgggcg
     1801 ccgcggccgg cttgctcgag gccaagaacg cggtcgaagc agcatttgag gagggcgatg
     1861 acgtcgaacc ggagctcgga actttgatct ctgctgcgaa cgaggcactc actgccgtct
     1921 cggaggagcc ggtcacatgt tcataagggc cgcacgtata gcgcttgccg cggtgatcgc
     1981 gacgatgctc atcgcttgtg gtggaaacga gcccgagccg cagggcggcc cgagcggacc
     2041 ctcgggcgag ttgatagttc aggtggcctc ttacgacatc gcggtggggg atgagggccg
     2101 cgtgatcctc ggtcttctca cccccgcgca gctgttcgtt tcttacggaa ccgtcgatgt
     2161 cgagttcttc tatctcggga ccgaagaagg gtcgggtaaa gcccagcccg gccccacgac
     2221 gacggggacc ttccttgtga tcccggggga tacagaaccg gagggggctg ccggccctat
     2281 cgctgcgccg gcatcgcgcg gccgtggcgt gtacgcgacc gaggtcgctt tcgatcgcgc
     2341 gggtttctgg tcggcacagg tgacggcaga tgtcgaggac gaaggccgct tgaccggcaa
     2401 cgccgtcttc gaggtgttga aggagcacgc gatcccggcc cccggcgatc ccgctctcaa
     2461 gaccgacacg ctgacgatcg actcgaagga tgcgcccgag gccgcagtgg attcccgcgc
     2521 agctcaagga gacatccccg atcccgagct gcatcagatg acgatcgccg aaacgatcca
     2581 gcgcggcgag cccgcactcg tcgtttttgc aacccccgtc tattgcgtct cacgcttctg
     2641 tgggccgatc accgacatgg tggccgatct cgcgaagaag tacgacgatc gcgcgaactt
     2701 catccacgtt gagatctgga ggaacttcga gggtcaagtc gtgaacaagg gagccgccga
     2761 ctggctcctg cgcggccagg atctcagcga gccgtgggtg ttcttgatcg gcgcggacgg
     2821 gaaggtgacc gctaggtggg acaacgtcgc cactccggaa gagatcgaac ctctgcttaa
     2881 gaagctgccc cggctgtgac cgtcgcgccg gcgcgggtgc acgatctaac gcgtgggctg
     2941 aaactcgagt acgtcagcct gggctacaac ctgatcgaaa cgggtgtggg catcaccgcc
     3001 ggcgtggccg cggggtcggt tgcgctgatc ggcttcggtc tcgactctct ggtcgaatcc
     3061 gcctcggccc tcatcctggt gtggcggttc aagaccgagg cgcgcggagc gcgcacctca
     3121 gaggaggccg aacgcagagc ggtcaagctg gtcgccgtcg ccttcttcgc gcttgccgcc
     3181 tacgtcggga tcaatgcggt cttcgaactg atccggggta tccggcccga gtcgagcccg
     3241 gtgggtatgg gcctcgccgt cgcctccctg atcgtgatgc cgtggctcgc acaccggaag
     3301 aggaagctcg cagacgagat gggtagtgct tccctccgtg ccgactcgcg ccagaccacg
     3361 ttgtgcacct atctctcggg cgttcttctc ttcgggctcg tcgcgaactc actcttgggc
     3421 tggtggtggg cggacccagt ggcggccgcg gcgatcgccg tcctcgcgct taccgagggg
     3481 gtcgagttgt ggcgaaccga ggacttctgc tgctgagcta gcgagactcg agcatcgcct
     3541 cgatgcttcg ttcgagctcg gtcgcatcga tgggtccgaa ccaaaccgtg ccctggcgat
     3601 tggcaacctc gtcctggctc gattcgccgg cgaaggtcca gttccgatcc acgaagtagg
     3661 tctccggtag tccgaggacg ccgaccggcc ctgcgaaggc ctcggccgga tcgtggatta
     3721 cgggatacgt gatgccgtag cgctcgacaa aatctttcgt gtcactggca gcatcccgga
     3781 tggcaacgcc aacgaatcgg acgccgcgat cctgatactt ctcgtacgtc cgttggagga
     3841 gcggcgcttc ctcgcggcaa ggcaggcacc acgatgcgaa gaaattcacg accacggggt
     3901 cgcccttcag gtcggcactc gaaagctcgc cactttcgtt caccaatgga agttcgaagt
     3961 ccggaaccgc tccaccggcc cccccgtctt tcgccggccg caggaatccg acgacgacaa
     4021 cggcgagaac gcaagcgatg gccgcgagga cgacgatctt gcgcgctcgt gagcggtggc
     4081 ggaaaccttc tagttcgttt gtaggaacca tgcggtgcta actattcggc caggttggtt
     4141 catatatatt ccgggcgtct atgaagcgcc cctcaagtgc caggcgaggc atcgcgacgc
     4201 tgtcgtcgtt cgcccttgta ttgcttgcag cgtgtgcgga taacccaccc caggatttcc
     4261 tcaatccgga ggggcccgtg gcgcgcgaag cggacaagct ttgggacatc acgttcctga
     4321 tcgctgccgt catcttcgtc atcgtccagg gcctccttgt ctttgcgatc ttccgctacc
     4381 gggcacgtcc tggggtcgag gcgaggcagt tccacggcaa caccaaggtc gaggtcatcc
     4441 tcaccgtcat cccggccctc atcttggccg gcctggccgt tcccacggtc aagaccatca
     4501 tgacgctgtc tgaaaaaccc acaggcaacg tcctcgagat caaggtcatc gctcgccagt
     4561 tctggtggga gtaccagtac tcggacctcg ggttcgttac cgcgaatgag atgcacgtcc
     4621 cggaggacac gcccgtctac ttgacgctcg aaggggacga cgtcatccac ag
//
LOCUS       DATMED010000156         8386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_494179, whole genome shotgun sequence.
ACCESSION   DATMED010000156 DATMED010000000
VERSION     DATMED010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494179"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VNP73_03900"
     CDS             <1..358
                     /locus_tag="VNP73_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWL65097.1"
                     /translation="LAWRMWEWRADRIVVTDRRIFEVSGVLTRKVASMPLVKMTDITY
                     RRSVLGRLLGYGELIVETAGQDQSLNRITHLPHPDDFYRTITWLVTGGLTPRAEPEDD
                     YYVDDDDTGPLPRVVV"
     gene            386..814
                     /locus_tag="VNP73_03905"
     CDS             386..814
                     /locus_tag="VNP73_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65098.1"
                     /translation="MASGIVAALIAVLTLGFEEGMRRFYPSKATWLGLRSKHGRRAVR
                     AMRERMDHAAENRLTRALATLLVGLVIIWVASASLLDKRWQEVVLDVLPTALVGIAMM
                     RTSSTLRRIAERMKDYERDLGEDPDRDYGTGDGGPAAIAL"
     gene            811..1437
                     /gene="thiE"
                     /locus_tag="VNP73_03910"
     CDS             811..1437
                     /gene="thiE"
                     /locus_tag="VNP73_03910"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWL65099.1"
                     /translation="MTLSDARLYLVAPARLAAGPLDELVPELVAAGVDLIQLREKNLE
                     AGDLIELGRPVAAACQRVGVPFIVNDRPDVAVALDADGVHLGQDDLPIDVARRVFPQA
                     FYGLSTHSAEQITGALLHKPDYIGVGPVNATPTKPGRPGTGMGLVAEAAELATVPWFI
                     TGGMNETTIPQALAAGGRRFVVVRAIVEAEDPVAATQKIRGVLDEVQL"
     gene            1449..2489
                     /gene="cax"
                     /locus_tag="VNP73_03915"
     CDS             1449..2489
                     /gene="cax"
                     /locus_tag="VNP73_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HWL65100.1"
                     /translation="MKPKLEWLLVFVPVAFVLEFTHANPVAIFVTAALAVLPLAVLMG
                     HATEELAVRSGPQLGGFLNATFGNLAELIIAYFLIVEGQLAIVKASLTGAIIGNVLLI
                     LGLSFLAGGWKRQQQTFSLASAELHSSTLVIAVIALMMPALFFASGDVTEFRTEAVSI
                     GVSVVLILTYAFSLIFSMKTHRSLFAQEEGHEEPKWSIRRSVTTLAVATIFVAVMSEF
                     LVGALNHTVEKLHLSPLFVGLIIVPLISNAAEHASAILLAMKDKMSVSIEIAIGSSVQ
                     IALFVAPVLVFLSLLTAHPLDLIFTGFEIAAVGFSSAILAFIALDGRSNWFEGVLLLA
                     AYVIMAISFFFL"
     gene            2508..3353
                     /locus_tag="VNP73_03920"
     CDS             2508..3353
                     /locus_tag="VNP73_03920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWL65101.1"
                     /translation="MPRAIDFHVHASTEEWLKGSIGPMLEVTARYFRSEVRVRTVEEM
                     ADEYKQWDLLGVLLGWDAETATGLPPLTNDRVAEICNAYPEQFIGFAGVDPHKPDAVE
                     ELDRAITELGLKGLKIHPQVQAFYPDDPKFDPLWDKCEEHSLPIVVHVGQTGLGAGVP
                     GGSGIAYDFGRPMLMDMVAARHPGLTVVMAHFGYPWHLEVLASAMHKTNVWVDLSGWR
                     PKYIPPEVKRDCRGRLSDRFVWGSDYPMLDPGRILDEFSEMELGDKEDDVLKNNAARL
                     LGIDI"
     gene            3358..3669
                     /locus_tag="VNP73_03925"
     CDS             3358..3669
                     /locus_tag="VNP73_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65102.1"
                     /translation="MAMFLVVRGQRGPDWDPSRSLEQQSGWAAHAAFMDDLVQRGNIV
                     LGGPAGERQAVLAFEAGSKDEVAELLAGDPWEGTHLITSSVEEWEIRLDGRPRVGESP
                     A"
     gene            3691..4005
                     /locus_tag="VNP73_03930"
     CDS             3691..4005
                     /locus_tag="VNP73_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HWL65103.1"
                     /translation="MATKEEIRNEVEAALKTVNDPELGIDIWNLGLVYEIAIDDANNV
                     KIDYTLTTMGCPIGPMIEEEVKGVTNDIEGIGELKMEMVMYPPWTPEKMSPLARSALG
                     FV"
     gene            4085..4771
                     /locus_tag="VNP73_03935"
     CDS             4085..4771
                     /locus_tag="VNP73_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65104.1"
                     /translation="MNFRKAASLLAVVAVLATAALMPASAGAKAKPVVVGTDPAGDWG
                     SNQDPSLGPLGGPLGQDVVKAEIAKVDAKTLNFIVTLASLPPNGGLPEGTRYTWHMVV
                     DGNYTELDGKWSNYSRGACDPTSGSCPPPRDPGMQPFVVRANCTADQVVICEEMGIVQ
                     ATFDPATATITIPVPLALIKAKTGSEIAHGVSEGSTFSGVAAAPSAFYSHATAPYDTL
                     TMTKSYVVPK"
     gene            4790..5947
                     /locus_tag="VNP73_03940"
     CDS             4790..5947
                     /locus_tag="VNP73_03940"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWL65105.1"
                     /translation="MPSRLDHLECAACGKRHDPAVVQQRCACGGTLLCRYNLSGLMLA
                     DVRSRPPGTWRYRELLPIEGDPVSLGEPETATLFAPRLSERWGIEAWIKDESTLPGAT
                     FKARGACVGLSRAVELGVKRVVMPTAGNAGGTWSLYAARAGIEITVVMSATAPRTNQL
                     EVTAAGGTLELVDGSIADAAVRAKEIADETGAFFAATFYEPYRLEGKKAAWLESFDSL
                     GDADSMSFPATIVLPVGGGVAAIAAAKVAAEVRELGWSNDRAPRLVGVQAANCAPIVK
                     AFEEGRDDVPPWTGDPHTVAAGLRVPAPIEGPLVMETIRASGGTMVAVEEDRIVESMR
                     DLAATEGLLACPEGATTVDAAEQLVRAGDLDGPVLLYNTGAGQKYLDVLTD"
     gene            complement(5944..6405)
                     /locus_tag="VNP73_03945"
     CDS             complement(5944..6405)
                     /locus_tag="VNP73_03945"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HWL65106.1"
                     /translation="MRILLLYAHIVLAILWIGGAVMFNLMATRARSSGSPEAVIRTMG
                     DSEWAGKFYFTPVAILTLVTGVVLVLTSNGAYTFEDPFVVAGIAGIVISGVLGGTYYA
                     KAAQSISAAVNERGLEDAGTRSQLDRLRLIAWVETAMLLGIELLMVYRAGS"
     gene            6662..7012
                     /locus_tag="VNP73_03950"
     CDS             6662..7012
                     /locus_tag="VNP73_03950"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HWL65107.1"
                     /translation="MAKRYDEQIEVSTTADDATPLSFTWRGRRYYVDERLSSWREGGR
                     WWHSERAVASASAQIERNAVYEREFFRVVAHQAGMGGTGELDPDGFVVTCSSVYDICL
                     DRIKGIWLMARLWD"
     gene            7037..7453
                     /locus_tag="VNP73_03955"
     CDS             7037..7453
                     /locus_tag="VNP73_03955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWL65108.1"
                     /translation="MRTRLDAYLAGDFTIPANDAFGFKLEAADDPTDHVSVSWTVPSK
                     YCNSAGNLQGGMFGAFVDAALGWACSAHIPADMYPALAEMKISFLRPATAGTTIHATA
                     RVIKPGKRLLFVEAEVTNDAGDLIAKVSATEVPAKA"
     gene            7458..>8386
                     /locus_tag="VNP73_03960"
     CDS             7458..>8386
                     /locus_tag="VNP73_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWL65109.1"
                     /translation="MSSFVHLHCHTTWSLLDGTIPAESLPHAARHLGFEAIAMTDHDS
                     LTGAVRFARACRAAGVKPIYGAELTLTDGSHVTAIARDAVGYGNLCRLITDSHMCNER
                     GKPGTTMAKVAERAEGLFVLSGCGSGEVARLAAAGALPQAEEAALQWRDAIGDGYRIE
                     VFDHRGYGDRMLRDRLLRIAKDTGIAAVATNDVHYPAPGDAGVHELLHAIKEIVPLSQ
                     TQALRHTSEFHLKDATEMAELFYDYPDAIDETWRIAEACNYDLDLEGYHFPEIAWLKG
                     ISATGELARRCFAGAQRRYGTVTREVDERLQHEL"
BASE COUNT         1501 a         2573 c         2770 g         1542 t
ORIGIN      
        1 gctcgcatgg cggatgtggg aatggcgtgc cgaccggatc gtcgtcaccg atagacgcat
       61 cttcgaagtc tcgggcgtac tcacgaggaa ggttgcctcg atgccgctcg tgaagatgac
      121 cgacatcact taccgccgtt ccgtgctggg caggctgctc gggtatgggg agttgatcgt
      181 cgagacagca ggccaggacc aatcgctcaa ccggatcacg catctccccc atcccgacga
      241 cttctaccgg acgatcacat ggctcgtgac gggtgggcta acgcccaggg ccgagccaga
      301 ggacgactac tacgtggacg acgacgacac ggggcccctg ccacgggtcg tcgtgtgaac
      361 gccgcgcact agctaccctg gagccatggc aagcgggatc gttgcagccc tcatagccgt
      421 cctcaccctg gggttcgagg aggggatgag gcggttctat ccatccaagg cgacgtggct
      481 cggcttgcgg agtaagcacg ggcgacgagc agtacgtgcc atgcgcgagc ggatggacca
      541 cgcggccgag aatcggctga cgcgtgcact cgcgacgctt ctggtggggc tcgtgatcat
      601 ctgggtcgcg tcggccagcc tcctagacaa acgctggcaa gaggtcgttc tcgatgttct
      661 tccgaccgcg ctcgtcggaa tcgcgatgat gcggacctca agcactctcc gccggatcgc
      721 ggagcgaatg aaggattatg agcgtgatct cggcgaggac ccggaccgag attacggaac
      781 cggagatgga ggaccggccg cgatcgcgct gtgactctct ccgatgcacg cctctatctg
      841 gtggctccgg cccggctcgc ggccggacca ctggatgagc tcgtgccgga actcgtagcg
      901 gccggggtcg acctgatcca gttacgggag aagaacttgg aggcgggaga tctgatcgag
      961 ctcgggcgac ccgtcgccgc cgcctgccag agagttggtg tccccttcat agtcaacgac
     1021 cgcccggacg tcgcggttgc cctcgatgcc gacggagtcc atctcggaca ggacgatcta
     1081 cccatcgatg ttgctcgcag agtgttcccg caggcgttct acggcctttc gacccattct
     1141 gcggaacaga taaccggcgc cttgctgcac aagcccgatt acatcggggt tggtcccgtc
     1201 aacgcgacgc cgaccaagcc ggggcgcccc ggcacaggca tgggactggt cgccgaggcg
     1261 gccgagcttg cgaccgtgcc gtggttcatc accggtggga tgaacgagac gaccatccct
     1321 caggcgctgg ccgcaggggg gcgcaggttc gtggtcgtgc gagcgatcgt ggaggctgag
     1381 gacccggtgg ccgccaccca gaagatccgc ggggtcctgg acgaggtcca gctttagtct
     1441 ccccggcgat gaaaccgaaa ctcgagtggt tgctcgtctt cgtaccggtt gccttcgtgc
     1501 tggagttcac gcatgcaaac ccggtggcga tcttcgtcac ggccgcgctt gcggttctgc
     1561 cgctggcggt cctgatgggt catgcgaccg aagagctcgc ggtgagatcg ggtcctcagc
     1621 tgggcggttt cctcaacgcg acgttcggca acctcgccga gctgatcatc gcctacttcc
     1681 tgatcgtcga gggtcagctc gcgatcgtga aggcgtcgtt aacgggggcg atcatcggga
     1741 acgtcttgtt gatcctcggc ttgtcgttcc tcgcgggcgg gtggaagcgg cagcagcaga
     1801 cgttctcgct ggcctcggcg gagctgcatt cgtcgacatt ggtgatcgct gtgatcgcgt
     1861 tgatgatgcc ggcgctgttc ttcgcttcgg gtgacgtgac ggagttccga accgaagccg
     1921 tctcgatcgg ggtctcggtc gttctgatcc tgacctacgc cttcagtctg atcttctcga
     1981 tgaagacaca ccgcagcttg ttcgcgcagg aggagggtca cgaggaaccg aaatggtcca
     2041 tcaggcggtc ggttacgacg ctcgcggttg cgacgatttt cgtagcggtg atgagcgagt
     2101 tcctcgtggg agccctcaat cacactgttg agaagctgca tctgtcgccg ttgttcgtgg
     2161 ggctgatcat cgtgccgttg atcagcaatg ctgccgagca cgcgtccgcg atcctgcttg
     2221 cgatgaagga caagatgagc gtctcgatcg agatagcgat cggctcatcg gtgcagatcg
     2281 ctctcttcgt ggcgccggtt ctggtgttcc tatcgcttct aaccgcgcat cccctcgatc
     2341 tgatcttcac gggcttcgag atcgcagcgg tcggtttctc gtcggcgatc cttgctttca
     2401 tcgcgctcga cggacgatcg aactggttcg aaggtgtctt gctactggcg gcttacgtca
     2461 tcatggcgat ctcattcttt ttcctgtaaa gccgtaagga ggctcacttg ccacgagcga
     2521 tcgatttcca cgtccacgct tcgaccgagg aatggctcaa gggctctatc ggcccgatgc
     2581 tcgaagtcac tgcgaggtac ttccgctccg aggttcgcgt gcgcaccgtc gaggagatgg
     2641 ccgacgagta caagcagtgg gatctcttgg gcgtcctgct cgggtgggac gcggagacgg
     2701 caaccggcct gccgccgttg acgaacgacc gcgttgcgga gatctgcaac gcgtatcccg
     2761 agcagttcat cggtttcgcc ggtgtcgatc cacacaagcc cgatgccgtc gaggaactcg
     2821 accgggcgat aaccgagctg ggtctcaagg ggttgaagat ccaccctcag gtgcaggcgt
     2881 tctatcccga cgacccgaag ttcgatccac tgtgggacaa gtgcgaagag cactcgctcc
     2941 cgatcgtcgt ccacgtcgga cagacggggc tgggggcagg ggttcctggg ggctcaggga
     3001 tcgcgtacga cttcggtcga ccgatgctga tggacatggt cgcggcccgc catccgggcc
     3061 tcacggtcgt tatggcgcac ttcggctatc cctggcacct ggaggtgctg gcgtcggcca
     3121 tgcacaagac gaacgtctgg gttgatctgt cggggtggcg tccgaagtac atcccaccgg
     3181 aggtcaaacg cgattgccgg ggtcgtctgt cggatcgctt cgtgtggggg agcgactacc
     3241 cgatgctcga cccgggacgg atcctggacg agttttccga gatggagctg ggcgataaag
     3301 aggacgacgt gctgaagaac aacgccgcgc gcctcctagg gatcgacatc taggttcatg
     3361 gcgatgttct tggtcgttcg gggtcaacgg gggccggatt gggacccgtc ccggtcactc
     3421 gagcagcagt ccgggtgggc cgcgcatgct gcatttatgg acgatctggt tcagcgcggc
     3481 aacatcgttc tcggagggcc ggcaggtgag cggcaggcgg tgcttgcttt cgaagccggg
     3541 tccaaggatg aagtggctga gctcctggcg ggcgatcctt gggaagggac gcacctcatc
     3601 acctcttccg tggaggagtg ggagatccgc ctcgacggac ggccccgagt cggggaatct
     3661 cctgcctaac tgcacggtta ccctgggtgt atggcaacta aggaagagat ccgcaacgag
     3721 gtagaagcgg ccctgaagac cgtcaacgac cccgagctgg gcatcgatat ctggaacctc
     3781 gggctcgtct acgagatcgc gatcgacgac gccaacaacg tcaagatcga ctacaccctg
     3841 acgaccatgg ggtgcccgat cgggcccatg atcgaagagg aagttaaggg cgtgaccaac
     3901 gacatcgagg gcatcggcga gttgaagatg gagatggtca tgtacccgcc ctggaccccc
     3961 gaaaagatgt cccctctggc taggtcggcc ctcggtttcg tctagaggtc caggcgcagg
     4021 aagctctatc taaacggcga atctccccag ctagacggca ctttttctat aagggggatt
     4081 cccgatgaac ttccgcaaag cagcgtcgct cctggcggtc gtggccgtac tcgcgaccgc
     4141 agctctcatg cccgcatcgg caggcgcgaa agccaagccc gtcgtcgtcg gcaccgaccc
     4201 cgcaggtgac tggggcagca atcaagatcc gagcttgggc cccctcggtg gacccctcgg
     4261 tcaggacgtc gttaaagccg agatcgccaa ggtcgacgcg aagacgctca acttcatcgt
     4321 caccctcgcc agcttgcctc ccaatggggg gcttcctgag ggcacccgct acacgtggca
     4381 catggtcgtg gacggcaact acaccgagct cgacggtaag tggagcaact actcccgtgg
     4441 agcttgtgac cccacctcgg gaagctgccc tccaccgcgg gatcccggga tgcagccgtt
     4501 cgtagtgcgc gcaaactgca cggccgatca ggtcgtgatc tgcgaggaga tggggatagt
     4561 tcaagccacg ttcgatccgg cgaccgcgac gatcacgatc ccggtgccgc ttgcgctgat
     4621 caaggcgaag accggctcgg agatcgctca cggtgtcagc gagggttcga cgttcagtgg
     4681 cgtcgctgcg gcgccttccg cgttctactc gcacgcgacg gctccgtacg acacgttgac
     4741 gatgaccaag tcgtacgtgg tgcctaaata acccaattaa cctcggtcga tgccttcccg
     4801 gctcgaccac ctcgaatgcg cggcctgcgg taagcgtcac gaccctgctg tagttcaaca
     4861 gcgttgtgct tgcggtggga cgttgctgtg ccgctacaac ctgagtggcc tgatgttggc
     4921 cgacgtccgc tcccggcccc ccgggacgtg gcggtatcgg gagttgcttc cgatcgaagg
     4981 cgatccggtg tcgttgggag aacccgagac ggcgacactc ttcgcacccc ggctctcgga
     5041 gcggtggggg atcgaggcgt ggatcaagga cgaatccacg cttcccggcg cgacgttcaa
     5101 agcgagaggt gcgtgcgtag gtctttcgcg tgccgtcgaa ctgggcgtga agcgggtcgt
     5161 aatgccgacc gccggcaatg ccggggggac gtggtccctc tacgcggccc gggcgggcat
     5221 cgagatcacg gtcgtgatgt cggcgaccgc gccgcgcacc aatcaactcg aggtgacggc
     5281 cgcgggcgga accctcgagc tcgttgacgg ctcgatcgcc gatgccgccg tcagggcgaa
     5341 ggagatcgcg gacgaaacgg gtgcgttctt cgcagctact ttctacgagc cgtatcgctt
     5401 ggagggcaaa aaggcggcgt ggctcgagtc gttcgattcg ctcggagacg ccgattcgat
     5461 gtcgttcccg gcaacgatcg tgcttcccgt cggtggggga gtcgcggcga tcgcggccgc
     5521 caaggtcgcc gccgaggtgc gcgaactcgg ttggagcaat gatcgggcac cgcgactggt
     5581 tggggtgcaa gcggcgaact gcgcgccgat agtgaaggca ttcgaagagg gccgggacga
     5641 tgtcccgccg tggaccggcg atccacacac ggtcgccgcg ggcctacgtg tccctgctcc
     5701 catagaggga ccgctcgtta tggagacgat cagagcttcc ggcgggacga tggtggcagt
     5761 cgaggaggac cggatcgtcg agtcgatgcg cgatctcgcc gcaacagaag gactcctggc
     5821 gtgccccgaa ggagcgacga ccgtggacgc ggccgagcag ctcgtccgag caggcgacct
     5881 agacggtccc gtgctcctct acaacactgg ggcgggtcag aagtatctag acgtgcttac
     5941 cgactaactt cccgctcggt agaccatcaa cagctcgatc ccgagcaaca tggcggtctc
     6001 gacccatgcg atcaggcgca agcggtcgag ctgtgatcgt gtgccggcat cctcgaggcc
     6061 ccgctcgttc accgcggcgg atatcgattg agccgccttg gcgtagtacg tgccacccag
     6121 cacgccggag ataacgatgc cggcgatccc tgcgacgacg aaggggtcct cgaacgtgta
     6181 agcgccgttc gaggtgagga cgaggactac accggtgacg agcgtgagga tcgcgacggg
     6241 ggtgaagtag aacttgcccg cccattcgga atcacccatg gtcctgatga cggcctcggg
     6301 gctacccgac gagcgagccc tagtggccat caggttgaac atcacggccc caccgatcca
     6361 caggatggcg agaacgatgt gggcgtagag aagcaagatc cgcatgggaa atgacctcct
     6421 gagagagccg agggctcagg ctaagccacg ctctatggcc ggcgatcgcc gagccgacag
     6481 ctgagccgct acttgactgg ctggggtcgc tccgctacca tcgaacatac gttcgataca
     6541 ggcaggccgc cggctctccc cgccggtatg ggaatccccg gcccccaggc ctcctgcatc
     6601 ggacaactac accggggccc gctgcaaagc gggccccccg cttttcggag aggcctgcaa
     6661 gttggcgaag cggtacgacg aacagatcga ggtgtcgacc accgcggacg atgccactcc
     6721 actcagtttc acctggcgcg gccggcgcta ttacgtcgac gagcgattgt cgtcctggcg
     6781 cgaggggggc cggtggtggc acagcgaacg cgcggtcgcc tcggcgagcg cgcagatcga
     6841 acgcaacgcc gtatacgagc gtgaattctt ccgcgtcgta gctcatcagg cggggatggg
     6901 tggtaccggc gagctcgatc ccgacggctt cgtcgtcacg tgctcgtcgg tctacgacat
     6961 ctgtctggat cgcatcaaag gcatctggct gatggcacgc ctatgggact gagcaggccc
     7021 cgatatcgtg cgccgtatgc gcacccgtct cgacgcgtac ctcgccggcg atttcacgat
     7081 ccccgcgaac gatgcgttcg gcttcaagct ggaggcagcc gacgacccga cggatcacgt
     7141 ctccgtgtcg tggacggttc cgtccaagta ttgcaactcg gccgggaatc ttcagggcgg
     7201 gatgttcgga gcgttcgtag acgctgctct ggggtgggcg tgctcggcgc acataccggc
     7261 cgacatgtac ccggcgctcg ccgagatgaa gatctcgttc ctgcgtccgg cgaccgccgg
     7321 tacgacgatc catgcgacgg cccgcgtgat caaacccggc aaacgtctgc tgttcgtgga
     7381 agccgaggtc acgaacgacg cgggcgacct gattgccaaa gtctcggcaa cggaggtccc
     7441 ggccaaggcg tagctcggtg agctctttcg ttcaccttca ctgccatacg acctggtcgt
     7501 tgctcgacgg cacgatcccg gccgagtcgt tgccgcatgc cgctcgccac ctcggcttcg
     7561 aagcgatcgc tatgaccgac cacgattcgt tgaccggcgc ggtccggttc gcacgggcct
     7621 gtcgcgctgc cggcgtcaaa ccgatctacg gcgccgagct cacgctcacc gacggctcgc
     7681 acgtcaccgc gatcgctaga gacgcggtcg gctacggcaa cctgtgccgg ctgatcaccg
     7741 actcgcacat gtgcaacgaa cgcggcaagc ccggcacgac gatggccaag gtcgccgagc
     7801 gtgccgaagg tttgttcgtg ctgtccggct gcggatcggg tgaggtcgcc cggctcgcgg
     7861 ccgccggcgc tttgcctcag gccgaagagg ctgcgttgca gtggcgggat gcgatcggtg
     7921 acgggtatcg gatcgaggtc ttcgatcacc gcggctacgg cgatcggatg cttcgggatc
     7981 gtttgctccg gatcgccaag gacaccggga tagcggcggt tgcgactaac gacgttcact
     8041 atccggcccc cggagatgcc ggcgtccacg agctcttgca cgcgatcaaa gagatcgtgc
     8101 ccctgtcgca gacgcaggcg ctccggcaca cgtccgagtt ccacttgaag gacgcgaccg
     8161 agatggccga gctcttctac gactatcccg atgcgatcga cgagacgtgg cggatcgcag
     8221 aggcgtgcaa ctacgacctg gatctcgagg gctaccactt cccggagatc gcgtggttga
     8281 agggcatcag cgcgaccggt gagctagcac gtcgttgctt cgccggcgca cagcgtcgtt
     8341 acggaacggt cacgcgcgag gtcgacgagc ggctccagca cgagct
//
LOCUS       DATMED010000157         5191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_495300, whole genome shotgun sequence.
ACCESSION   DATMED010000157 DATMED010000000
VERSION     DATMED010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495300"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /gene="nagA"
                     /locus_tag="VNP73_03965"
     CDS             <1..989
                     /gene="nagA"
                     /locus_tag="VNP73_03965"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HWL65110.1"
                     /translation="AVPGYVDLQVNGFAGVDFLSADAEGYREAGLAIAATGVTAYQPT
                     FITSPLEAYWPALDTVAGVDDTGGPRLLGVHLEGPFISPSWPGAHRAGHILEPDLAIA
                     DRLCAAGPVTFMTVAPEQPGGLKLVTHLVEKGLVVACGHTDSDSDVARRAFDAGARAL
                     THIYNAQRRWKPRDPGIAGVALTRPDIVVQAIVDHVHLAPETAYATFLATRGRFCLVT
                     DAMAATALGDGIYSLGDREVSVTGTEARLADGTLAGSVLTMDQAVQNLVGLGASIEDA
                     VDAATRVPAQLVGKHDLGFLTEGGTADLVVLDEALMVERTLVGGTEVFARAS"
     gene            complement(986..1426)
                     /locus_tag="VNP73_03970"
     CDS             complement(986..1426)
                     /locus_tag="VNP73_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HWL65111.1"
                     /translation="MVAVSSPFTGRVVPLDEVDDEVFSERIMGDGVAVHPTSGEVVAP
                     LSGRIEKLFKGGHGLAIEDDEGLQILVHVGLETVRLKGDGFTVHAAEGDTVSTGDLIV
                     TVDLDRMNELEIDLVSPVVVISGHGVTNQATGEVASGDRLFDAG"
     gene            complement(1426..1671)
                     /locus_tag="VNP73_03975"
     CDS             complement(1426..1671)
                     /locus_tag="VNP73_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose/sucrose transporter subunit IIB"
                     /protein_id="HWL65112.1"
                     /translation="MDLRSTAEQVLVGLGGKDNIKALDPCITRLRVELHDMSKLDEPA
                     LKQAGAHGVMKMGGAVQVVMGTQSDNIEAEMRRIMEA"
     gene            complement(1674..2570)
                     /locus_tag="VNP73_03980"
     CDS             complement(1674..2570)
                     /locus_tag="VNP73_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HWL65113.1"
                     /translation="MTRLLDLWGRELAELEGESLRDAILQSEGRVVMAEVIAHVPPLL
                     GGTSNPELVAAFGADLICLNMMDPRAPGPLIEGVEGVTPTPESFSDLTRFLGRPVGLN
                     LEPDLESVPATYRSSESNISAADAGGAAFVMITANPGRGAGIADIAQSVGVVRKSAPH
                     LLCLAGKMHQAGADERLDARAVGRVMDAGAHGVLIPLPGTVPGIDEATAAGMAAASHE
                     AGGIAVGTIGTSQEGAEIETIRSLALAAKRIGVDVHHIGDAGYTGIAIPENVYAYSLA
                     LRGRRHTWNRMARGTRGSWGGS"
     gene            complement(2567..4243)
                     /gene="ptsP"
                     /locus_tag="VNP73_03985"
     CDS             complement(2567..4243)
                     /gene="ptsP"
                     /locus_tag="VNP73_03985"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWL65114.1"
                     /translation="MTKIEGTAASPGACVAPAVLLVSEQLDLPSGPVEQREPEISSLE
                     KALERTAQQLEQRASDSSGELVEVLEAQAMMARDPELSEAARKAIQDDGTPAARAIVD
                     AAETFAEALASSDSEYMAARATDVRDVAKLIARSLLGMPEVDLAQLQKPSVIVASDVS
                     PADVARLDFEVVKGFATEEGSRTSHTAIVARSFGVPAVVAAAGLVGAVKEGDVVGVDG
                     DAGVVYVKPDEVLIGELESRGERRAQAQQELASVDVVHTRTSDGHEVELAVNVGSLKE
                     LETARRMGARAVGLLRTELVFFDRQEAPTEKEHAELMTAMASLLDGGRLVIRTFDFGA
                     DKPMPFLEVSGTSDSALGVRGIRLARAQPELLETQLRAIATVADSGASIAVMAPMVAT
                     IEEVDWFVDRARECGVVDAGAELGVMVEVPSAVFLAAELAARLDFLSIGTNDLVQYLH
                     AADRREGSLAPLQDPFAPALLRAVNAVCDAAGEGAWIGVCGEAAADPAWALTAIGLGV
                     TELSMGAGSLADAHAAIGAITMDDCSRMAQRVLLAEDASEAGAIARELLP"
     gene            complement(4240..4494)
                     /locus_tag="VNP73_03990"
     CDS             complement(4240..4494)
                     /locus_tag="VNP73_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWL65115.1"
                     /translation="MPERNVTLENEVGLHARPAAVFSKAAARFSSSVTVVKSGAEGNA
                     KSVLSVLKLDIQKGDSVTIRADGPDEEEALTELVKLVEDL"
     gene            complement(4521..>5191)
                     /locus_tag="VNP73_03995"
     CDS             complement(4521..>5191)
                     /locus_tag="VNP73_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWL65116.1"
                     /translation="AITNTPDSPLGDVADMMLELGAGPEEAVPATKTFSAQVVAFALI
                     AEALGAVPWQGGDWVRVRSQVEQVLGDDGPAQQVAEVIGDAEGLISVGRGFLYSAALE
                     AALKLKETTSILAEGYSSADLRHGPIAVATSGLPILAFVAPGPAASDMHELMGWLDQR
                     GARVLAVSEEPGAALPLPSGLPEMLVVVPAIVRAQQLALWLARHRDIDPDAPAGLSKV
                     TFTQ"
BASE COUNT          943 a         1769 c         1538 g          941 t
ORIGIN      
        1 ttgcggttcc cggctacgtc gacctccagg tgaatggttt tgcgggggtg gactttctga
       61 gtgccgacgc cgaggggtac agggaggccg gccttgcgat cgcggcaacc ggagtaacgg
      121 cctatcaacc gaccttcatc acctccccac tcgaggccta ctggcccgcc ctcgatacgg
      181 ttgcgggagt cgatgacacc ggcggaccgc gcctgctcgg agtgcatctc gagggtccct
      241 tcatctcgcc aagctggccg ggcgctcatc gagccgggca catcctcgag cccgacctcg
      301 caatcgccga ccgattgtgc gccgcaggac cggtcacgtt catgactgtc gcacccgagc
      361 agcccggcgg tctgaagctc gtcactcatc tcgtggaaaa gggattggtc gtcgcctgtg
      421 ggcacaccga ttcggacagt gatgtcgcgc gccgcgcctt cgatgccggt gcgcgcgccc
      481 tgactcacat ctacaacgcc cagcggcggt ggaaaccgcg cgaccctggt atcgccggcg
      541 tcgcgctgac gcgtcccgat atcgtggtgc aggcgattgt cgatcacgtg cacctcgccc
      601 cggagactgc gtacgccacc ttccttgcga ccagaggacg attctgtctc gtgaccgacg
      661 cgatggctgc caccgctctt ggtgacggta tctacagcct cggtgaccgc gaggtcagcg
      721 tgacgggtac cgaggcgcgc ctcgcggacg gaaccctcgc gggaagcgtg ctgacgatgg
      781 accaggcggt tcagaatctt gttggtctgg gggcgtcgat cgaggacgca gtcgacgccg
      841 caacgcgcgt tccggcacaa ctcgtcggta aacatgacct tggctttctt accgagggtg
      901 gaacggcgga tctcgtggtc ctagacgaag ccctcatggt cgaacgaact ctagtcggag
      961 ggaccgaggt cttcgctcgc gcttcttaac cggcgtcgaa caaccgatct cccgatgcaa
     1021 cctcgcccgt cgcctggttc gtcacgccgt ggccagatat caccacgacg ggagagacaa
     1081 gatcgatttc caattcgttc atccggtcca ggtcgacagt gacgatgagg tcgccggtgc
     1141 tgacggtatc tccctccgca gcgtgaaccg tgaaaccgtc gcccttcaac ctcacggtct
     1201 cgaggccgac gtgaaccagg atctgaagac cttcatcgtc ttcaatagca agtccatgtc
     1261 cacctttgaa gagcttctcg atgcgccccg atagaggcgc gacgacctca ccggaagtgg
     1321 gatgaaccgc gaccccgtcg cccatgatcc gttcggagaa aacctcgtcg tcgacttcgt
     1381 cgagcggaac cacgcgtccc gtgaaggggc ttgaaacggc aaccatcacg cttccatgat
     1441 gcggcgcatc tcggcttcga tattgtcgct ctgcgttccc atcacgactt gaacggcgcc
     1501 gcccatcttc atgaccccat gggcaccagc ctgtttcagc gcaggttcat cgagtttgct
     1561 catgtcatga agttcgacac gaagccgcgt gatacacgga tccagagcct tgatgttgtc
     1621 cttgcccccc aggccgacca gaacctgttc cgcagtagat cgaagatcca tactcagctc
     1681 cctccccatg aacccctagt gcctcgtgcc atccgattcc acgtgtgccg tctgccgcgc
     1741 agggcaagcg agtacgcgta aacattctcc ggtatcgcaa tgcccgtgta acccgcatcg
     1801 ccgatgtgat ggacatcgac cccgattcgc ttggcggcta gcgcgagcga gcggatcgtc
     1861 tcgatctcgg ccccctcctg tgacgtgccg atggttccca cggcgattcc tccggcctcg
     1921 tggctggcgg cagccatgcc cgccgccgtc gcctcatcga tgccgggaac cgttccggga
     1981 agggggatca ggacaccgtg cgcgcccgcg tccatgaccc tgccgacggc tctcgcgtcg
     2041 agtcgttcgt cggccccggc ctggtgcatc tttcccgcaa gacagaggag gtgtggggcg
     2101 ctcttccgga cgacccctac cgattgagca atgtcggcaa tccccgcacc tcggcccgga
     2161 ttggcggtga tcatcacgaa agccgcgcca ccggcgtctg ctgcgctgat gttcgactcg
     2221 ctggatctat aggtcgccgg gacgctctcg agatccggtt cgagattcag tcccaccggg
     2281 cggcccaaga aacgagtgag atccgagaaa gactcgggag ttggggtgac tccttcgacg
     2341 ccctcgatga gaggcccggg cgccctcggg tccatcatgt tcaagcagat gaggtcggcg
     2401 ccgaaagcgg ccaccaactc gggattggaa gtaccgccga gcaacggggg gacgtgcgcg
     2461 attacctctg ccatcacaac tcgaccctcc gattggagta tggcgtcgcg caacgactcg
     2521 ccctccagct cggccaactc gcggccccag agatcgagca gccgtgtcat ggcaacaact
     2581 cccgcgcaat cgcaccagct tcggaggcgt cctcggcgag gagaacacgt tgcgccatcc
     2641 tgctgcaatc atccatcgtg attgctccta tagcggcgtg cgcgtctgcg aggcttccgg
     2701 cccccatcga tagctccgtg actccgagtc caatagcggt gagggcccac gcaggatcgg
     2761 cggcagcctc tccgcagact ccgatccacg caccttcgcc ggcggcgtcg caaaccgcgt
     2821 tcacggctcg cagcagcgca ggagcaaaag gatcctgcaa cggcgccaac gagccctcgc
     2881 gccgatcggc tgcgtgaagg tactgaacca gatcgttcgt gccgatcgaa aggaagtcga
     2941 gtcgtgcggc cagctcggcc gcgagaaaaa cggctgacgg tacctcgacc atcacgccaa
     3001 gttcggcccc cgcgtcgacg accccacact cgcgcgcgcg gtcgacaaac cagtcgacct
     3061 cctcaatcgt cgccaccatc ggagccatca ccgcgatgct ggcacccgag tctgcgaccg
     3121 tcgcaatggc gcgcaactgc gtttcgagaa gctccggctg agcacgcgcc aggcgaatcc
     3181 cacgcactcc gagagctgag tctgacgtgc ccgacacttc gaggaacggc atcggtttgt
     3241 cggccccgaa atcgaaagtg cgaatcacca agcggccccc atcaagcaac gacgccattg
     3301 ctgtcatcag ctcggcgtgt tccttttcag ttggcgcttc ctgtcgatcg aagaacacga
     3361 gttccgtgcg caacagccca acggctcgtg cacccattcg ccgcgcggtt tcgagctcct
     3421 tcaaactccc gacattcacg gccagctcga cctcatggcc atcactcgtt cgagtgtgaa
     3481 cgacgtccac ggatgccagc tcctgttgag cctgggcgcg ccgttcgccg cgagactcga
     3541 gttcgccgat cagcacctcg tcaggcttga cgtaaacaac tcccgcatct ccgtctacgc
     3601 cgacaacgtc accttccttg accgcgccga ccaatcccgc cgcagccact acggcaggaa
     3661 cgccaaagga ccgcgcaacg atggctgtat gactggttct actgccctcc tccgtcgcaa
     3721 atcctttgac aacctcgaag tcgaggcgcg cgacatcagc cggggagaca tcgctcgcca
     3781 ctatgaccga aggtttctgc agctgcgcga ggtcgacctc cggcatcccg agcaacgagc
     3841 gcgcgatgag cttcgcaaca tcgcgcacat cagtggcgcg cgccgccatg tactcgctgt
     3901 cactggacgc gagcgcttcg gcgaaggtct ctgcggcgtc aacgatcgct ctggcagcgg
     3961 gcgtcccatc gtcctggatc gccttacggg cggcttcaga caattcggga tcgcgagcca
     4021 tcatcgcctg cgcttcgagg acctccacca gctcaccaga agagtccgac gcccgctgct
     4081 ccagctgctg cgcggttctc tcgagcgcct tctccaaaga ggagatctca ggctcccgct
     4141 gctcgacggg cccagacggc agatcgagct gttcgctcac aagtaggacg gcaggcgcaa
     4201 cgcaggcacc gggggaggca gctgtgccct cgatcttggt cacaggtctt cgacgagctt
     4261 caccagctct gtcagagctt cctcttcgtc tgggccgtca gctcgaatcg tgacgctgtc
     4321 tcccttttgg atgtcgagct tgagcacgga tagcacggac tttgcattcc cctcggcgcc
     4381 cgacttcacg acggtgaccg acgaagagaa ccgcgccgcg gctttgctga ataccgcagc
     4441 cggacgggcg tgcagaccta cttcgttctc gagtgtcaca ttgcgttcgg gcatgccctc
     4501 tctcctgatc tccgcattct ctactgagtg aacgtcacct tactgaggcc tgctggggca
     4561 tccggatcga tgtcccggtg gcgtgcaagc catagcgcaa gttgctgcgc gcgcacgatt
     4621 gcggggacga cgacgagcat ctcgggaagt ccgcttggca gcggcagagc cgcacccggc
     4681 tcctccgaga cggccagaac acgcgcccct ctttgatcga gccagcccat gagctcgtgc
     4741 atgtcggatg cggccggccc gggagcgacg aacgcgagta tggggaggcc gctagtagcg
     4801 accgcgatgg ggccgtggcg gaggtcggcc gacgagtatc cctcggccag aatcgaggtg
     4861 gtctccttca gctttagagc ggcctccaac gccgcactgt agaggaaccc gcgcccgacg
     4921 ctgatgaggc cctctgcgtc accgatgacc tccgcgactt gctgcgccgg gccatcatcg
     4981 cccagcacct gctccacttg actgcggacc cgtacccagt cgccgccctg ccagggcacc
     5041 gcaccgagag cctctgcaat gagagcgaag gccacgacct gcgcactgaa ggtcttggtt
     5101 gcgggcacgg cttcctcggg cccggctccc aactccagca tcatgtcggc gacgtctccg
     5161 agcggcgagt cgggggtgtt ggtgatcgcc a
//
LOCUS       DATMED010000158         2703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_496035, whole genome shotgun sequence.
ACCESSION   DATMED010000158 DATMED010000000
VERSION     DATMED010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496035"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1379
                     /locus_tag="VNP73_04000"
     CDS             159..1379
                     /locus_tag="VNP73_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWL65117.1"
                     /translation="MSKAQPPRLPSGERLRRAGVGAWSIIGILVLTAITVWLLFKVRV
                     IFPPLVLALLIIYLLNPFVNRLEERKVPRTVGAVLCYVVVGLIITLVGTIVAPFLSRQ
                     VQNFSARAPELQLSLIHTVEAFGNSVEDRFGIHLETSEVSCLLGADEIDEVQSPTTAR
                     CDEVTEALREEVLDRTDRATEFAGSIFEVLLIFILAPLLALYLLIDLPHLQRDIVNLA
                     PEHLRDELSDLGSKVGRAVGGFFRGQLLVALLVGIMSSFGFWAVGLDFWLPIGAIAGF
                     TNLIPLVGPFIGGSLGFLVGTITEDIRVGLLAALVALIVQQIDNHVISPNVMRRTVQL
                     HPVTVMLSLLAGGTLAGFWGVLLAVPGVAVAKLLLGHLWATRVLGVPPSPFSPVPAGS
                     PPSVVSKDAAPEKD"
     gene            complement(1376..2341)
                     /locus_tag="VNP73_04005"
     CDS             complement(1376..2341)
                     /locus_tag="VNP73_04005"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HWL65118.1"
                     /translation="MSQHIFDPVARGWFERRTFSYPDLDPTSLIQAKQDRNLSVAVCC
                     PALNEAVTIGPICARIRDSVRAAGLVDELLVIDSGSTDGTRVEATDAGATVFSVDELV
                     TQVPPAERGKGDSLWRSLAAVRSDIVVWLDADLEDFDERFITHLLAPLLLRTDVSISK
                     GFYERPIGDGDRTQRVGGARVTELTARPLLNLLWPELSGFVQPLSGEYAGYTSTLRTL
                     PFFIGYGVEVGLLIDASTRLGIDAVAQVDLGVRRHRNRPVLDLGATALEVIGAILNRA
                     EDLGRLKLAEELTAKMVQFVPDGDTFLPRIIEGTIGERPPLDSVL"
     gene            2373..>2703
                     /locus_tag="VNP73_04010"
     CDS             2373..>2703
                     /locus_tag="VNP73_04010"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674169.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWL65119.1"
                     /translation="MKYVRGLACRECGREYPVAAQHVCEFCFGPLEVVYDYEAIAAEV
                     TRESIAAGPQTIWRYESLLPAPAEARINIGAGWTRLREAPRLAAELGLKRLWLKNDGG
                     NPTHSFKD"
BASE COUNT          460 a          855 c          841 g          547 t
ORIGIN      
        1 cttcgggcca ctgaatcaag ggccgccgcc aggcccccgg ccccccgttt ggttgcgaaa
       61 ccgccgcctt ccgccacgaa agagatcggc ccacgattgg acacgaatgt gattgccgtc
      121 tcttcggtca tgaggttcgt atggtaggag gcgaatcgat gtcgaaagcc caacctcctc
      181 gacttccctc cggcgagaga ctgcgccgtg ccggtgtcgg tgcgtggtcg atcatcggga
      241 tccttgtcct tacggcgatc accgtgtggc tgctgttcaa ggttcgcgtg atcttccccc
      301 cgctcgtgct cgccctgctg atcatctatc tgctgaaccc gttcgtgaac cgtctggaag
      361 agcgaaaggt gcctcggacc gttggagcgg tgctctgcta tgtcgtcgtc ggcttgatca
      421 tcaccctcgt cgggacgatc gtggccccct tcctctcgcg ccaggtccag aacttcagtg
      481 cccgggctcc cgaacttcag ctgtcactga tccacacggt ggaagccttc ggcaacagcg
      541 tcgaagatcg ttttgggatc catctcgaaa cgtccgaggt gtcgtgtctt ctgggggcag
      601 acgagatcga cgaagtccag tcacccacga cggctcgctg cgacgaggtc accgaggccc
      661 ttagggaaga ggtgctggat cgaacggaca gagctaccga gtttgccggc tcgatctttg
      721 aggttctact catcttcatc ctggcgccgt tgctggcgct gtacctattg atagaccttc
      781 cgcatctgca gcgtgacatc gtgaacctgg ccccggaaca tctgcgggac gagctgtccg
      841 acctcggatc gaaggtcggg cgagcggtcg gcggtttctt ccgtggccag cttctcgtcg
      901 ccttactcgt cgggatcatg tcgtcgttcg ggttctgggc cgtcgggctg gatttctggc
      961 ttccgatcgg cgccatcgcg ggtttcacga acctgatacc tctcgtggga ccgttcatcg
     1021 gcggaagcct cggattcctg gtagggacga tcaccgagga cataagggtc gggctcttag
     1081 ccgccctggt cgcgttgatc gttcagcaga tcgacaacca cgtcatcagt ccgaacgtca
     1141 tgcgcaggac ggttcagctc catcccgtca cggtcatgct ctcgttgctc gccggtggca
     1201 ccctggcagg cttctggggg gtcttgctcg cggttcccgg tgtggccgtc gcgaagcttc
     1261 tcctcggtca tctgtgggca acgcgcgtgc ttggtgtgcc gccctcgccg ttctctccgg
     1321 tcccggcggg gtctcctccc tctgtcgttt cgaaggacgc ggcgccggag aaggactaga
     1381 gaaccgaatc cagcggcggt cgttcgccga tcgtgccctc gatgatccgg ggcaggaagg
     1441 tgtcaccatc gggaacgaac tggaccatct tggcggtgag ctcttcggca agcttgaggc
     1501 ggcccaggtc ctctgcacga ttcaagatcg ctccgatcac ctccagcgcg gtagcgccga
     1561 gatcgaggac cggtcggttg cgatgtctcc gcaccccgag gtcgacctgc gcaacagcgt
     1621 cgatcccgag ccgcgtcgac gcatcgatca gcagtccgac ctcaaccccg tacccgatga
     1681 agaagggaag cgtcctcaag gtcgacgtgt atcccgcgta ctcgcccgac agcggttgca
     1741 cgaatccact cagttcgggc cacaggagat tgaggagtgg tcgggccgtc agctccgtga
     1801 cccgggcacc acccacgcgc tgtgtccggt ccccatcccc gataggccgc tcgtagaacc
     1861 ccttgctgat ggagacgtcg gtgcgaagca gcagcggcgc caggaggtgc gtgatgaatc
     1921 tctcgtcgaa gtcctcgaga tcggcatcga gccacacgac gatgtcggat ctcacggcgg
     1981 cgagactcct ccacagcgag tcgcccttac cccgttcggc cggtggcact tgagtgacga
     2041 gttcgtcgac cgagaagacg gtggcgccgg cgtcggtcgc ttccacccgt gtgccgtccg
     2101 tcgagcctga atcgatgacg aggagttcgt cgacaaggcc tgccgcccgt actgagtccc
     2161 ggatgcgggc gcagatcggt cctatcgtga cggcttcgtt gagcgcaggg cagcagacgg
     2221 ccacggagag gttccggtct tgcttggctt ggatcagaga ggtcggatcc aggtccgggt
     2281 aactgaacgt ccttcgttcg aaccagcctc gtgctacggg gtcaaagatg tgttgcgaca
     2341 ccgctcgacc ctagcgcccc tagaatctct caatgaaata cgtcagaggt ctggcatgca
     2401 gagagtgcgg ccgcgagtat ccggttgcag ctcagcatgt atgcgaattc tgcttcggcc
     2461 ctctcgaggt cgtatacgac tacgaagcga tcgcggctga ggtcacccga gagtcgatcg
     2521 ccgccggccc ccagacgatc tggcgttacg aatctctcct acccgcgccg gccgaggccc
     2581 ggatcaacat cggagccggg tggacgcgac tcagagaagc tcctcggctc gcggcggagc
     2641 tcggattgaa gcgcctctgg ttgaagaacg atggaggcaa ccccacgcac tcgttcaagg
     2701 acc
//
LOCUS       DATMED010000159         3395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5486205, whole genome shotgun sequence.
ACCESSION   DATMED010000159 DATMED010000000
VERSION     DATMED010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5486205"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VNP73_04015"
     CDS             complement(<1..261)
                     /locus_tag="VNP73_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65120.1"
                     /translation="MATASVEAAQVSKVSRTEKLKTAVKKLQVRAGWREVERWLLILG
                     AFLLIFGVAAIGLAWWGASHTPFVFEQIPYLISGGLLGLGLAF"
     gene            complement(262..1578)
                     /locus_tag="VNP73_04020"
     CDS             complement(262..1578)
                     /locus_tag="VNP73_04020"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HWL65121.1"
                     /translation="MIKRLLALAAVVTIFSMTFIGAAESAPEGLIITATASGSTWEYN
                     DPDSPIPATPSAEMHEAYSLASFDSGPSSHGLSSVFWPGSAGANFGPVYGLPAYPVRA
                     ETFYPKGPEKNAMEPGPPGLSMKAEATEEFASATASTGAEDGSPGAAARRLVSSVTSS
                     VADENGVSEATATVKDLNIGDGAITIESVVTKARITTDGKKATVTGKTTVTGVMVADQ
                     FEASIDEKGLHINDQVVEIPDVFGEGPAKEALDQLGISVKVARPITKIKGGSALTELG
                     GLVVKFDALALVPYIEPLPDEIEDEIYKNVDMRKETVYRFGSVSLGVDASDAGFELPE
                     LPPVDPPAPAPPADLSVGGGGAPAVGIPGVGGGDVPSFDAAPEAAPQEQPTLAAEPAS
                     ITAHPLPASILVLTLLAAIGSTRVMRKMADQLVGGSAMACPHKDVG"
     gene            complement(1578..2300)
                     /locus_tag="VNP73_04025"
     CDS             complement(1578..2300)
                     /locus_tag="VNP73_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65122.1"
                     /translation="MPRETAAPILELIDVYAAYGRITILHGVDLVVPEGSVVALLGPN
                     GAGKTTTIKVAAGQLRPKSGCLHVAGRHVNGTTPDALARAGVCTIPEGRGIFPNLTVR
                     ENLRMVTYSSASLSTIEERSFERFPILAERRSQLAGTLSGGEQQMLSIARALAVDPTL
                     LMLDEISMGLAPKIVEELYELVAQIAADGVSILLVEQYAHMAMAVADYVAVMAHGRVT
                     AMGEPGDMEEELHSAYFGTAAA"
     gene            complement(2290..3027)
                     /locus_tag="VNP73_04030"
     CDS             complement(2290..3027)
                     /locus_tag="VNP73_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65123.1"
                     /translation="MALLQAEGIEVRFGGLHALKGVDLEVEAGGITGLIGPNGAGKTT
                     FFNVVCGLQSPTAGRVLVEGKDVGKLKAHRRARLGIGRTFQRLEVFSSMTVLENVLTG
                     AEIRKSWSRDRSSPREIAQEVLTRLHIEDFAEERADSLPTGLLRIVELGRALATRPRL
                     LLLDEPSSGLNQSESHLLASLLKSEANAGLGVLLVEHHIELVMEVCRDIFVLDFGSIL
                     ARGTPDEIQNNEAVQRAYLGKGVNHAA"
     gene            complement(3008..>3395)
                     /locus_tag="VNP73_04035"
     CDS             complement(3008..>3395)
                     /locus_tag="VNP73_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="inner-membrane translocator"
                     /protein_id="HWL65124.1"
                     /translation="SSAMAGIGGAMFGGLQGSAGPTDFLMFQSLPVLLMAVIGGITTV
                     SGGLIGGLLLAMLPVLQGEGPAVGGLVFLATGAAAVSLGRNPNGIAYLIAQLLSPLAF
                     WKRRSRRRPLIPDKPEEVTGVGVAAG"
BASE COUNT          631 a         1127 c         1034 g          603 t
ORIGIN      
        1 gaaggcgagg cctagtccga gaaggccgcc ggagatcagg taagggatct gctcgaacac
       61 gaacggcgta tgcgatgccc cccaccatgc cagtccgatc gcagcaaccc cgaagatcag
      121 taggaacgct ccgaggatca acagccaccg ctcgacctcg cgccaaccgg ctcgtacctg
      181 cagcttcttg acggcggtct tcagcttctc cgtccgcgag accttgctta cttgggccgc
      241 ctctacagat gcagtggcca tctaccccac gtccttgtgt ggacatgcca tcgcgcttcc
      301 ccccaccaat tggtcggcca tcttcctcat cacccgggtc gacccgatcg cggcgagaag
      361 agtgagaacg aggatcgaag caggaagtgg atgggcagtt atcgaggcag gctctgccgc
      421 aagcgtcggc tgctcctggg gtgcagcctc gggagcagca tcgaaagacg ggacgtcgcc
      481 gccaccgaca cccgggatac cgaccgctgg agcgccgccc ccacccactg acaggtcggc
      541 gggcggagcg ggagcaggcg ggtcgaccgg tggcaactcc ggcagctcga acccggcatc
      601 gctggcatct acacctaggc tcacagaacc gaagcgatag acggtttcct tgcgcatgtc
      661 gacgttcttg tagatctcat cctcgatctc gtccggaaga ggctcgatgt aaggcaccag
      721 cgctaaggcg tcgaacttaa cgacgaggcc tcccaactct gtcagagcgg agccgccctt
      781 gatcttggtg atgggtcggg caaccttcac gctgatgccg agctggtcca gcgcctcctt
      841 tgccggtccc tcaccgaaca cgtcggggat ctcgacgacc tggtcgttga tgtgcaggcc
      901 cttctcgtcg atcgaagcct cgaactggtc tgcaaccatc acgccggtga ccgtggtctt
      961 cccggtgacc gtggccttct tcccatctgt cgtgatgcgg gctttggtaa cgaccgactc
     1021 gatcgtgatc gcgccgtcac cgatgttcag atccttgacc gttgcggtgg cctcgctgac
     1081 gccgttctca tctgcaaccg agctggtgac tgacgagacg agccggcgcg cagctgcacc
     1141 cggtgatccg tcctcggccc ccgtcgacgc cgtggctgaa gcgaactcct ccgttgcctc
     1201 ggccttcatg ctcagcccgg gaggacccgg ctccatcgcg ttcttctctg ggcccttcgg
     1261 atagaacgtc tcggctctaa cgggatacgc gggcaagccg tacaccggcc cgaagttcgc
     1321 gccggccgaa cccggccaga acacgctcga cagtccgtgg ctcgaagggc ccgagtcgaa
     1381 gctcgcgaga ctgtatgcct cgtgcatctc cgcactgggc gtcgcaggga tcggcgaatc
     1441 gggatcgttg tactcccacg tcgaacccga tgcggtcgcg gtgatgatga gaccttccgg
     1501 cgcgctctcg gccgcaccga tgaacgtcat cgagaagatg gtgacgaccg cagctagggc
     1561 gagaaggcgc ttgatcatca cgccgccgcc gttccgaagt aggcgctgtg cagctcttct
     1621 tccatatccc caggctctcc catggccgtg acacgaccgt gtgccatcac ggccacgtaa
     1681 tcggcaacag ccatcgccat gtgagcgtat tgctcgacga gcaagatcga aaccccgtcg
     1741 gctgcgatct gggcgaccag ctcgtagagc tcctccacga tcttgggggc caggcccatc
     1801 gagatctcat ccagcatgag gagggtcggg tcgaccgcca gcgccctcgc gatggacagc
     1861 atctgttgct cgccaccgga cagcgtgccg gccagttgcg agcgtcgttc ggccaggatc
     1921 gggaatcgtt cgaagctgcg ctcctcgatc gtcgaaagcg atgcagatga gtaggtcacc
     1981 atccgcaggt tctcgcgcac ggtgaggttc gggaagatcc ctcgtccctc cgggatagtg
     2041 cacacccccg cgcgtgcgag cgcgtcgggc gtcgtgccgt tgacgtgacg accggcaacg
     2101 tgaaggcaac cactcttggg tctcagttgt ccggcagcga ccttgattgt cgtggtcttg
     2161 ccggctccgt tggggccgag cagcgcaacg acgcttccct cggggacgac gaggtcgacc
     2221 ccatggagaa tggtgatccg tccgtacgcg gcatacacgt cgatcaactc gaggatcggt
     2281 gcggcagtct cacgcggcat ggtttacacc tttgcctaga taggcgcgct gcacggcttc
     2341 gttgttctgg atctcgtccg gggttcctcg ggccaggatg gaaccgaagt ccagcacgaa
     2401 gatgtcccgg cagacctcca ttaccaactc aatatggtgt tccaccagaa gcacccctag
     2461 acctgcattg gcctccgatt taagaaggga ggccagcagg tgcgattcgg actgattcag
     2521 ccccgaggag ggctcgtcca ggagcaaaag gcgggggcga gtggccagcg cccgacccag
     2581 ctcgacgatc cgcaagaggc ccgtgggcag cgaatcggcc ctctcctcag cgaagtcctc
     2641 gatatgcaat cgggtcagca cttcctgggc tatctcccga gggctcgaac gatcccggga
     2701 ccacgatttt cggatctcgg cccccgtgag gacgttctcc agaacggtca tcgagctgaa
     2761 aacctcgagg cgctggaagg tccgcccgat gcccaggcga gctcgccggt gagccttgag
     2821 tttgccaacg tctttgcctt cgacgaggac gcgacccgcc gtcggtgatt gcagcccgca
     2881 cacgacattg aagaacgtcg tcttcccggc gccgttcgga ccgatcaggc ccgtgatccc
     2941 cccggcttcc acttcgaggt cgacaccctt gagcgcgtgg aggccaccga atcgaacttc
     3001 gatgccttca gcctgcagca acgccaacgc ccgtcacctc ctccggcttg tcggggatca
     3061 gaggacggcg gcgggaacgt cgcttccaga aggcgagagg gcttagcaac tgcgcgatca
     3121 gatatgcgat cccgttcggg ttgcggccga gactgacggc agctgcgccg gtagcaagga
     3181 agacgagtcc gccgaccgcg ggcccctctc cttgcagcac cgggagcatc gccaacaaca
     3241 gaccaccgat cagaccgccc gagaccgtgg ttatccctcc gatcaccgcc atgagcagta
     3301 ccggcaggct ctggaacatc aagaagtcgg tcggacccgc cgaaccttgc aatccgccga
     3361 acatcgcccc gccgatgccg gccatggccg aggac
//
LOCUS       DATMED010000160         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5487339, whole genome shotgun sequence.
ACCESSION   DATMED010000160 DATMED010000000
VERSION     DATMED010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5487339"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1452)
                     /locus_tag="VNP73_04040"
     CDS             complement(55..1452)
                     /locus_tag="VNP73_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65125.1"
                     /translation="MFDGEDEGSDPGSGSSGPPSTPQPSPTTDKPGDPSIDLGEADLP
                     EDHPIKHVVFIVKENRTYDNYFARYPGGEGTRTGQTSDGRTVKLAVAPDVQKPDLGHS
                     FFDGIISINGGKMNGFDRILNGDDLGGYNSFKRSGMPNYWAYADNFVLGDHHFSSMYG
                     PTFPEHLYTVGGQAGDVVGNKLQTNSPGGYCDDPGETVYRFIKMKKKEQREVMRAEER
                     VDLDRVGDFWEEVRACFNFEVIQDQLDQHDISWHYYADEGSWMNALLAIKHMRFSKHW
                     GKDITEEEQFMKDITSGNLDEVTWVVPGPGVNEHPGGPSVCMGENWTVEHVNAIMRSK
                     YWKNTVIFITWDDFGGYYDHVPPPHTDHMGLGPRSPFLVISPWAKEGFIDDTVTEPSS
                     VLALVEEIHGLDCLTHRDCNASNLAQVFDFDQPATAPKDRKLILDERSCEGLPARIVS
                     EYERHGDDAFRALGD"
     gene            1667..2188
                     /locus_tag="VNP73_04045"
     CDS             1667..2188
                     /locus_tag="VNP73_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65126.1"
                     /translation="MSDHLSDPPPYQPPPPEDDFYEWENEEEGPRPTLLWTRIVALIA
                     GLLLVFFLGRMTAGGDDDGNDAELATLRQENSQLEAQVSDLQADLDAAQSPEPETTET
                     PAEGEGEGEEDTTTEAVDTYTVQAGQGFQQIAEDAMGDFGFAECIAEFNDMTLESTIL
                     PGDELDIPAEEDC"
     gene            complement(2190..2852)
                     /locus_tag="VNP73_04050"
     CDS             complement(2190..2852)
                     /locus_tag="VNP73_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65127.1"
                     /translation="MFRACVVGIGQGPTGDEAIDRTSEKSVEAGGPVLSPLRAFVVLL
                     AFAVVAGGVIVAATTDRPSPTRDSRKEAGVVSPSQTPSDPAPDESGSELSEGEARALF
                     GRLRHRLEVAYRRRSVEVLLEVVGRGSPQHSQSRSDLRLLERNNLLDRTRSRTLDVSI
                     VAIEPGRVVVSERTDVRPLYIDDATYVGVDVELGRTRSRSEWTLERWDDRWVITSSRA
                     TG"
     gene            complement(2866..>3096)
                     /locus_tag="VNP73_04055"
     CDS             complement(2866..>3096)
                     /locus_tag="VNP73_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65128.1"
                     /translation="LLLDLTIYRGEIVGNVAALPGWAGVGFRILACVRTKVLEVISVE
                     LLSSRSPVAGTHGEAPLSPLQARDYSRLNRMF"
BASE COUNT          582 a          991 c          945 g          578 t
ORIGIN      
        1 atccggcgct tcagaagagc gcgctaagaa cgcgcatcgc gaacccttcg tcccctagtc
       61 ccccaaagcg cggaaggcat cgtccccgtg gcgctcgtac tcagagacga tcctggccgg
      121 aagcccctcg cacgaacgct cgtcgaggat caacttcctg tccttcggtg cggtggcggg
      181 ttgatcgaaa tcgaagacct gagcgaggtt gctggcgttg cagtcgcggt gcgtcaggca
      241 gtcgaggccg tgtatctcct cgacaagagc caggacagaa gacggctcgg tgacggtgtc
      301 gtcaatgaag ccctccttgg cccacggcga gatgacgagg aacggcgatc gggggcccag
      361 ccccatgtgg tccgtgtgcg gcggcggaac atgatcgtag tagccgccga agtcgtccca
      421 ggtgatgaag atcaccgtgt tcttccagta cttgcttcgc atgattgcgt tcacgtgctc
      481 gacggtccag ttctcgccca tgcacacgct cggaccgccc gggtgctcgt tcactccggg
      541 gccgggcacc acccacgtca cctcgtcgag gttgccggag gttatgtctt tcatgaactg
      601 ttcctcctcg gtgatgtcct tgccccagtg ctttgaaaag cgcatgtgct tgatcgccag
      661 cagcgcgttc atccacgagc cctcgtcggc gtagtagtgc caggagatgt cgtgctggtc
      721 cagctggtcc tgaatgacct cgaagttgaa acaggctcgg acctcctccc agaagtcccc
      781 aactctgtcg agatccacgc gctcctcggc tctcatcacc tcccgttgct ccttcttttt
      841 catttttatg aacctgtaga cggtctcgcc cggatcgtcg cagtagccgc ccggcgaatt
      901 ggtctgaagc ttgttgccga caacgtctcc cgcctggcca cccactgtgt agagatgctc
      961 gggaaaggtc ggcccgtaca tcgagctgaa gtggtgatcg ccgagaacga agttgtcggc
     1021 ataggcccag tagttcggca tccccgatcg cttgaacgag ttgtatccac cgaggtcgtc
     1081 gccgttcaag atgcggtcga aaccgttcat cttgcctccg ttgatcgaga tgatcccatc
     1141 gaaaaagctg tggccgaggt caggcttctg cacgtctgga gctaccgcca acttgacggt
     1201 tcgcccatcg gaggtttgcc ccgtgcgggt tccctcccca ccggggtaac gcgcgaagta
     1261 attgtcgtag gtgcgattct ctttgacgat gaagacgacg tgcttgatcg ggtggtcctc
     1321 tggaaggtct gcctcgccga ggtcgatcga ggggtcgccg ggtttgtccg ttgtgggaga
     1381 tggctggggc gtggagggcg ggcccgagct ccctgatcct ggatccgagc cctcgtcctc
     1441 tccgtcgaac atctggcagg ctccagccag gccaaagagg gccacgacga gggcaatacc
     1501 ggctcgccgg gtgctcctcc gtaacagtcc agtggtgccg accacatatt ttccttccag
     1561 ttggggtagt tccgggaaca tacgtacact gaaccggctc tgaaatcgag gatggattcg
     1621 ggctacgctt ggatccgaca acccccaaag gaacagacga acaataatga gcgaccatct
     1681 gagcgatccg ccgccatacc aacctcctcc acccgaggac gacttttacg agtgggagaa
     1741 cgaggaggag ggcccccggc ccacccttct ttggacgcgc atcgttgctc tcatcgccgg
     1801 gttgctcctt gtcttcttcc tgggtcgcat gactgccggc ggtgacgacg acggtaacga
     1861 cgccgagctg gccactctgc ggcaagagaa ctcacagctc gaggctcagg taagcgacct
     1921 ccaggctgac ctggatgcag cgcagtcgcc ggagccggag accaccgaga ccccagcaga
     1981 gggcgaaggt gagggtgagg aagacacgac caccgaggct gtcgacacct acacggttca
     2041 ggccggccag gggttccagc agatcgcgga ggacgctatg ggcgacttcg ggttcgcgga
     2101 gtgcatcgcc gagttcaacg acatgacgct cgagagcacg atccttcccg gcgacgagct
     2161 cgacatcccg gccgaggagg actgctgagc taaccggtcg cgcggctcga agtaatgacc
     2221 cagcgatcgt cccatcgctc gagcgtccat tcggatctcg acctcgttcg gccaagctcc
     2281 acatcgaccc cgacgtacgt cgcatcgtcg atgtagagcg gtctcacgtc ggtgcgctca
     2341 ctcaccacca cccggcccgg ctcgattgca acgatggaca cgtcgagcgt gcgcgagcga
     2401 gtccgatcaa gaaggttgtt ccgctcgagc aggcgcaggt cgctccggct ctgagagtgc
     2461 tgaggcgacc cacgacccac gacctcgagc aggacctcga cgctcctccg acggtaggcc
     2521 acctcgagcc tgtgccgaag ccggccgaag agtgctcggg cctcgccctc actgagctcg
     2581 ctcccgctct catcgggtgc cgggtcggac ggcgtctggg aaggactcac cactccggcc
     2641 tccttccggg agtcccgggt ggggctcggc cgatccgtgg tggccgcgac gatcacgccc
     2701 cccgcgacga cggcgaaggc caggagcacg acgaacgcac gcagcggact cagcaccggt
     2761 ccaccggcct cgacactctt ctcgctcgtt cggtcgatgg cttcatcccc cgtcggtcct
     2821 tgaccgatac ctaccacgca cgcccgaaac aagtcgcgcg acttcctaga acatgcggtt
     2881 caatcgcgag tagtctcggg cttggagcgg cgaaaggggg gcctcaccat gggttccggc
     2941 aacgggcgaa cgggaactca gaagctcaac cgatatgacc tcgaggactt tagtcctaac
     3001 gcaagcgagg atacggaaac cgaccccggc ccatccgggc agagcggcga cattgccgac
     3061 gatctcgcct ctgtagatcg tgaggtcgag aagcag
//
LOCUS       DATMED010000161         6245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2433605, whole genome shotgun sequence.
ACCESSION   DATMED010000161 DATMED010000000
VERSION     DATMED010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433605"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VNP73_04060"
     CDS             complement(<1..325)
                     /locus_tag="VNP73_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HWL65129.1"
                     /translation="MIAYILVGVIALAVTFTATPVVQAFARRVGAIDQPSDRKVHANP
                     TPTLGGMAIFVGLVAAGIVAFFIPEFELAFIRSSALIGVLAGAVVIFALGAVDDLRDL
                     PAPVKL"
     gene            complement(359..1621)
                     /gene="glyA"
                     /locus_tag="VNP73_04065"
     CDS             complement(359..1621)
                     /gene="glyA"
                     /locus_tag="VNP73_04065"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWL65130.1"
                     /translation="MTQPGWSDWAAVERIDPEVADAILDEINRQNTKIELIASENFSS
                     PAVLAALGTPLTNKYAEGYPGKRYYGGCEFVDVAENLAIQRAKALFGAQHVNVQPHAG
                     AQANLAAYFAFIEPGDKFMGMELAHGGHLTHGSHVNFTGKLFDIVSYGVRKEDELIDY
                     DEVRDIAKREKPKLIVAGATAYSRLWDFATFRQIAEEVGAIFLVDAAHFIGLVAGGAH
                     PNPVDHAHVVTCTTHKTLRGPRGAMIMCEEQFAQQIDKSVFPGWQGGPLMHSIAAKAI
                     AFAEAATTEFKEYAHQVVANAKVMAETLTNEGLRIVSGGTDNHLMLVDLQPVGLTGKE
                     AEKRLDDVGITVNKNAIPYDPEKPFIASGIRVGTPAITTCGMKEDETEQVARLIAQCL
                     KDDSPGTQEEVGNKVRSLTERFRPYPEA"
     gene            complement(1618..2070)
                     /gene="rpiB"
                     /locus_tag="VNP73_04070"
     CDS             complement(1618..2070)
                     /gene="rpiB"
                     /locus_tag="VNP73_04070"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWL65131.1"
                     /translation="MRIALGADHAGYPLKLAVAKHLVDHGHEVIDLGTDSEESVDYPP
                     ICAAVGRSVVGGEADLGIVIGGSGQGEQIAANKVHGVRAALCLSEFTARLAREHNDAN
                     VLSLGGRILADAYAMAIVDVFLTTGFEGGRHERRVNQVAEIEKEECGK"
     gene            complement(2124..2645)
                     /locus_tag="VNP73_04075"
     CDS             complement(2124..2645)
                     /locus_tag="VNP73_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65132.1"
                     /translation="MKVVFVCTGNLCRSPMAEVLLRDALAQRDCTDVEVSSVGTWAMP
                     GSGATAIAVDVMRDMGIDLSMHSSRDAELEELEAADLIVVMTSVHQRELRGLGPHLMD
                     KVVMLKELAEIQMTEGAADPTARLAALLSGQRPKPRRSLDVDDPMGLPRGAYERTAAE
                     LRAGTDFLADLLC"
     gene            complement(2642..3226)
                     /locus_tag="VNP73_04080"
     CDS             complement(2642..3226)
                     /locus_tag="VNP73_04080"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWL65133.1"
                     /translation="MTELETAVAALRAGKLAVIPTDTVYGIAALPEIRDAVEAIFAAK
                     GRPPDKPLPVLGTDKTSLRHVARIGWGAEKLIDHYWPGPLTLVLERATGFDHFLGGDD
                     KSVAVRVPESDVARSLLAKTGPLAVTSANRSGAAPATTVAEAQQIFGSVVEVYVDGGA
                     ASGEPSTVVSLLGEPDVLREAAISAKDIMKVLVW"
     gene            complement(3223..4008)
                     /gene="prmC"
                     /locus_tag="VNP73_04085"
     CDS             complement(3223..4008)
                     /gene="prmC"
                     /locus_tag="VNP73_04085"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWL65134.1"
                     /translation="MLTARFGDITEVLRQAGIDNADREAQWIFEAADENEESALELAR
                     RRAGGEPLQYVTGTAAFRHIEVAVGPGVLIPRPETEVVADRALGLLPVGGTLVDIGTG
                     SGAIALAVAHERSDVTVFATEISDEAVIWAEKNRRALRSSVRLLKGDLFSPLPASLKE
                     QVDVVVSNPPYVALAERALLPSDVINHEPYEALFAEDGLSVLRRLIADSPEWLKPGGW
                     LVMEIGEKQGDDVLKLLSEAGFAGAFLEQDLTGRDRIAGASRK"
     gene            complement(4002..5075)
                     /gene="prfA"
                     /locus_tag="VNP73_04090"
     CDS             complement(4002..5075)
                     /gene="prfA"
                     /locus_tag="VNP73_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWL65135.1"
                     /translation="MKIPTDRLVEIESRFDEIEKALADPATAADTNHLRELGKEHAEL
                     RPIVEAWRPHQAAVADVTEARQMLSGASAEEKQYLQAEIDEQMGKIAELEARLTELLV
                     PKDPNDDRDVIVEIRAGAGGDEAGLFAAELFRMFQRYAEDKKWKAEVLSANESGVGGF
                     KEVIFAVKGKGAYSRLKHEGGVHRVQRVPVTESSGRIHTSAVGVLVLPEAEEVDVEID
                     PNDLRIDVYRSSGPGGQSVNTTDSAVRIKHLPTGVEVACQDEKSQLQNKEKGMRILRA
                     RLLQLKQEEQQKEQAEQRKSQVRTADRSERIRTYNFPENRVTDHRIGYTVHRLSEILE
                     GDLDDLIDALVASRRDEELGEAS"
     gene            complement(5084..6028)
                     /locus_tag="VNP73_04095"
     CDS             complement(5084..6028)
                     /locus_tag="VNP73_04095"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWL65136.1"
                     /translation="MSQATKPRPSIGGQAVLEGVMMRGPASWSVACRKPDESIAVEKH
                     DLPTLAQRHTWLRWPFFRGVLVLGESLAIGIKALMISANHALEEEEQLSDKQLGWTMT
                     VAMVMFSAVFIALPVLGTRALETWVIGDLSKTNPLAFNLIEGAFRIGVFVGYLLLIST
                     FKDIRRVFQYHGAEHKTIYAYENGDDLVPEEIDRNYSTLHVRCGTNFLFIVLFLTILA
                     HFIIDLAVGGPLIPRLAIRIAAIPVLAGFSYEVIKLASRNEDSIFFRVSMLPGLALQR
                     ITTKPPTLDQIEVAIAAMQAVAQDIDEGEADAPAAPVS"
     gene            complement(6044..>6245)
                     /gene="rpmE"
                     /locus_tag="VNP73_04100"
     CDS             complement(6044..>6245)
                     /gene="rpmE"
                     /locus_tag="VNP73_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWL65137.1"
                     /translation="PFYTGKQKLVDTGGRVERFQKKYAARPRRAAAPVPEAAPVPEAA
                     PVAATEPTDAPAEETANEETPA"
BASE COUNT         1104 a         2026 c         1920 g         1195 t
ORIGIN      
        1 caagcttcac cggcgcgggc aggtcccgaa gatcgtccac cgcgcccaaa gcgaagatca
       61 cgacggcacc ggccaacaca ccgatcaaag cggacgacct gatgaacgcc agttcgaact
      121 ccgggatgaa gaacgcaacg atccctgcag caacgagacc cacgaatatg gccatcccac
      181 cgagggtcgg cgtcggattc gcatgcacct tgcggtcgct cggttggtcg atggcgccga
      241 cacgccgggc gaaggcttgc acgaccgggg tggccgtgaa cgtcaccgcc aaggcgatga
      301 caccaaccaa gatgtaggcg atcaccggat caactcaccc ctctgtgatc gatccgccct
      361 acgcttccgg gtagggccgg aagcgttcgg tcaacgacct cactttgttg ccgacctcct
      421 cttgggtgcc gggtgaatcg tccttcaagc attgagcgat cagccgagcc acctgttcgg
      481 tctcgtcttc cttcatcccg caggtcgtga tcgcaggggt tccgaccctg atgcccgatg
      541 cgatgaaggg cttctcgggg tcgtacggga tcgcgttctt gttgaccgtt atcccgacgt
      601 catccaggcg cttctcggcc tccttgccgg tcaagcccac cggctggagg tccacgagca
      661 tcaagtggtt gtcggtgccc cccgaaacga tccggagtcc ctcgttcgta agcgtctcgg
      721 ccatcacctt tgcgttggcg acgacctggt gtgcgtactc cttgaactct gttgttgcgg
      781 cctcggcgaa agcgatcgcc ttcgccgcga tcgaatgcat caaggggccg ccttgccagc
      841 cggggaaaac actcttgtcg atctgctgag cgaactgctc ctcgcacatg atcatggcgc
      901 cgcgcggccc ccgcaatgtc ttatgcgtcg tgcaggtgac tacgtgtgca tggtcgacgg
      961 gattcggatg cgcgcctccg gctacgagac cgatgaaatg ggccgcatcg accaggaaga
     1021 tggcgccgac ctcctccgcg atctgccgga aggtcgcgaa gtcccacagg cgcgagtagg
     1081 ccgttgctcc ggcgacgatg agcttcggct tctcgcgctt ggcgatatcc cgaacctcgt
     1141 cgtagtcgat cagttcgtct tccttacgca cgccgtagga aacgatgtcg aagagcttgc
     1201 cggtgaagtt cacgtgtgaa ccgtgtgtca ggtggccacc gtgagccagt tccataccca
     1261 tgaacttgtc gcccggctcg atgaacgcga agtaggcagc gaggttggct tgggcccctg
     1321 catgtggttg gacgttgacg tgctgcgcgc cgaaaagagc cttggcacgc tggatcgcca
     1381 ggttctcggc aacatcgacg aactcacagc cgccgtaata gcgcttcccg ggatagccct
     1441 cggcgtactt gttggtcagc ggcgttccca acgcggccag gaccgcgggg gacgagaagt
     1501 tctccgacgc gatcagctcg atcttggtgt tctgccggtt gatctcgtcg aggatcgcgt
     1561 cggcaacctc gggatcgatc ctctctaccg cggcccagtc ggaccaccca ggttgagtca
     1621 cttgccacac tcctctttct cgatctccgc gacctgattg acccggcgtt cgtggcgccc
     1681 gccctcgaac ccggttgtga ggaacacgtc cacgatagcc atcgcgtagg catcggccag
     1741 gatccgcccc ccgagggaca ggacgttcgc gtcgttgtgc tcccgagcaa gccgagccgt
     1801 gaactcgctc aggcacagcg ccgcccgcac cccatggacc ttattggcag cgatctgctc
     1861 gccttgccca gacccgccta tgacgatccc caaatcggcc tcgccaccca ccaccgatcg
     1921 gcccaccgca gcgcagatcg gtgggtaatc gacggattct tcggaatcgg tgccgaggtc
     1981 gatcacctcg tgaccgtggt cgacgaggtg cttggctacg gcgagtttaa gcgggtagcc
     2041 ggcgtgatcg gctcccagag cgatcctcat ggagtcagtc taagaggggg ccgggcggga
     2101 gtgacgtttt cgcgcttcgg cgtttagcag aggaggtcgg ccaggaagtc ggtgccggcc
     2161 cgaagctctg cggccgtccg ctcgtaggcg ccgcgcggca agcccatcgg gtcgtccaca
     2221 tccagcgacc gccgcggttt cggtcgctgc ccggacagca gcgccgccag acgagcggtg
     2281 gggtccgctg cgccttcggt catctggatc tccgccaact ccttgagcat gacgaccttg
     2341 tccatgaggt gggggccgag gccgcgcagc tcgcgctggt gcacgctcgt catcacgacg
     2401 atcaggtccg cggcctccag ttcctccagc tcggcgtccc gggaactgtg catggagaga
     2461 tcgatcccca tgtctctcat gacatcgacg gcgatggcgg tcgccccgga cccgggcatg
     2521 gcccacgtgc cgacggacga cacctccacg tcggtgcaat cgcgttgtgc gagcgcgtcg
     2581 cgcaacagaa cctcggccat cggggagcga cacaggttcc ccgtgcacac aaaaacgacc
     2641 ttcaccagac aaggactttc atgatgtcct ttgcgctgat cgcagcctca cgcagcacat
     2701 cgggttctcc tagaagggag acgaccgtcg aaggctcgcc gctcgccgcc ccgccgtcga
     2761 catacacctc gaccacggag ccgaagatct gctgtgcctc ggccacggtc gttgccgggg
     2821 cggcacccga acggttggcc gacgtcaccg ccaacggacc ggtcttcgca aggagcgacc
     2881 gtgcgacatc agactccggg acacgaaccg caacactctt atcgtcaccg ccgaggaaat
     2941 ggtcgaaccc cgtggctctt tcgagcacga gggtgagggg gccgggccag tagtggtcga
     3001 tgagtttctc tgccccccag ccgatccgcg cgacgtgtcg caaggaagtt ttatcggtac
     3061 cgagtaccgg caacggctta tccggaggtc gtcccttggc cgcgaagata gcttccacgg
     3121 catctctgat ctcgggtaac gcggcgatgc cgtacacggt gtcggtaggg ataacggcga
     3181 gcttgccggc acgaagggca gccaccgcag tttcgagttc cgtcacttac gactggctcc
     3241 cgcgatgcga tcccggccgg tgaggtcctg ttccaggaac gctccggcga aaccggcctc
     3301 ggacagaagc ttcaagacat cgtctccttg cttctcaccg atctccatga cgagccatcc
     3361 gccgggcttc aaccactccg gcgaatcggc gatcaaccga cgtagtacgg agagtccgtc
     3421 ctcggcgaag agggcctcgt agggctcgtg attgatcacg tcgctcggca gtagcgctcg
     3481 ctcggccagc gcgacgtagg gggggttcga cacgacgacg tcgacctgct ctttcaagga
     3541 tgccggcaag ggagagaaga ggtcgccttt cagcaagcgc accgaagaac gaagggcccg
     3601 gcgattcttc tcggcccata tgacagcctc gtccgagatc tccgtcgcga acaccgttac
     3661 atcgctgcgt tcgtgtgcca cggcgagagc tatggcgccc gagcccgtac cgatatcgac
     3721 gagggtgccc cccacgggca acaggcccaa tgccctgtcg gcgacgacct ccgtttcggg
     3781 tcgggggatc aacacgccgg gacccacggc tacttcgatg tggcggaacg cggctgtgcc
     3841 ggttacgtat tgaagtggct cgccccccgc gcgcctacgc gcgagctcga gcgcggattc
     3901 ctcgttttca tcggccgctt cgaatatcca ctgcgcttcg cggtcggcgt tgtcgatgcc
     3961 ggcctggcgg agaacctcag tgatgtcgcc gaagcgcgcg gtcaggatgc ctccccgagc
     4021 tcctcatctc gacgactcgc gaccagtgcg tcgatcagat cgtcgaggtc gccctccagg
     4081 atctcgctca aacgatggac cgtgtatccg atgcgatggt ccgtcactct gttctcgggg
     4141 aagttatagg tgcggatcct ctcggagcgg tccgccgtcc gaacctgaga cttcctctgt
     4201 tccgcctgct ccttctgctg ttcttcctgt ttcagctgaa gcaaacgggc acggaggatc
     4261 cgcatcccct tctctttgtt ctgtagctgt gacttctcgt cctgacatgc aacttcgact
     4321 ccggtcggca ggtgctttat ccgcaccgca gagtcggtcg tgttgaccga ttgcccgccg
     4381 ggaccgctcg agcgatagac gtcgatgcga aggtcgttcg gatcgatctc gacgtcgacc
     4441 tcttcggctt cgggaagtac gagtacgccc accgcggacg tgtgtattcg tccgctcgat
     4501 tccgtaaccg ggaccctttg cacgcggtgg acaccgcctt cgtgcttgag gcgcgagtac
     4561 gcgccctttc ccttgacggc gaagatgact tccttgaagc caccgacgcc gctctcgttc
     4621 gccgacagaa cctcggcctt ccatttcttg tcctcggcat agcgctggaa catgcggaag
     4681 agctcggcag cgaacaaccc tgcttcgtcc ccaccggcac ccgcccggat ctcgacgatc
     4741 acgtcccggt cgtcgttcgg gtccttcggc accaacagct cggtcaagcg agcttcgagc
     4801 tcggcgatct tgcccatctg ctcgtcgatc tcggcctgga gatactgctt ctcctcggcc
     4861 gaagcgccgc tcagcatctg tcgagcttcg gtgacgtcgg caaccgccgc ctgatggggg
     4921 cgccacgctt cgacgatcgg cctgagctcg gcgtgttcct taccgagctc gcgtaagtgg
     4981 ttggtgtccg ccgccgttgc agggtcggca agggctttct cgatctcgtc gaaccgggac
     5041 tcgatctcga ctaaacgatc cgtagggatc ttcatggccg tagttaagaa accggggccg
     5101 cgggggcgtc tgcctccccc tcatctatgt cttgagcgac ggcctgcatc gccgctatcg
     5161 ccacctcgat ctggtcgagc gtcggtggct tcgtcgtgat cctctgcagg gccaggccgg
     5221 ggagcatcga gacacggaag aagatcgaat cttcgttgcg ggacgcgagc ttgatcacct
     5281 cgtaagagaa accggccaac accgggatag ccgcgatgcg gatcgcgagt cgagggatca
     5341 gcggcccccc gactgcgagg tcgatgatga agtgggcaag gatcgtcagg aacagcacta
     5401 tgaagaggaa gttagtgccg caccgaacgt gaagggtcga gtagttgcgg tcgatctcct
     5461 cggggaccag gtcgtcaccg ttttcgtacg cgtagatcgt tttgtgctcg gcaccgtggt
     5521 actggaaaac gcgacggatg tccttgaacg tggagatgag caacaggtaa ccgacgaaaa
     5581 caccgatcct gaaagcgccc tcgataaggt tgaaagcgag agggttcgtc ttcgacagat
     5641 ccccgatcac ccaggtctct agcgctctcg taccgagaac gggaagcgcg atgaataccg
     5701 ccgagaacat caccatcgcg acggtcatcg tccaaccgag ttgcttgtcg gacagctgct
     5761 cctcttcttc cagcgcgtgg ttcgccgaga tcatcagcgc cttgatcccg atcgcgaggg
     5821 actcgccgag caccaggaca ccgcggaaga agggccatct gagccacgtg tgtcgctgtg
     5881 cgagcgtcgg taagtcgtgc ttctcgaccg cgatcgactc gtccggttta cgacatgcaa
     5941 cggaccacga cgccgggccg cgcatcatta cgccttcgag gacggcttgg cccccgatgg
     6001 acggacgcgg cttagttgct tgactcacga accatcacct ccgctacgcg ggggtttctt
     6061 cgttcgcggt ctcctcggca ggggcgtctg tgggttcggt cgccgcgacc ggagccgcct
     6121 cggggacggg agccgcctcg gggacgggag ccgcggcgcg acggggacgc gcggcgtact
     6181 tcttctggaa acgctcgacg cgaccgccgg tatcgaccag cttctgcttg cccgtgtaga
     6241 aggga
//
LOCUS       DATMED010000162         4287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1269148, whole genome shotgun sequence.
ACCESSION   DATMED010000162 DATMED010000000
VERSION     DATMED010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269148"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /gene="truB"
                     /locus_tag="VNP73_04105"
     CDS             complement(<1..566)
                     /gene="truB"
                     /locus_tag="VNP73_04105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWL65138.1"
                     /translation="MTDGVLVVDKPAGITSHDVVDRIRKLFKTKRVGHAGTLDPEATG
                     VLILGLGRATRILSYAQAGPKSYRAQGKLGITTTTLDASGDEIDVRPVDIGRTELETA
                     VGSFVGEIEQVPPMVSAVKIGGERLYKKARRGEEVERPPRSVTIHSAEVISFDPPLFE
                     FDVRCSGGTYVRTLIADIGEKLGCGAHMT"
     gene            complement(563..1522)
                     /locus_tag="VNP73_04110"
     CDS             complement(563..1522)
                     /locus_tag="VNP73_04110"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWL65139.1"
                     /translation="MERDWKGAVDALQGANRVAVACHVNPDGDALGSLFASAIALKRL
                     GKEVWPSWGTPGAEVPSGYRFLPGNDLFVAPEEVPDCDVFLALDCGGSDRLGGLEEKA
                     SKAPVLINIDHHPGNSDFGTYNIVATQASSTAELVGRLLLDLGIEIDVAIATCLYTGI
                     VTDTGRFSYSNSSPDTLRMAADLLTSGVDAPAIALEVFESAPFGYLKLVGRVLDRAVL
                     HGAERFVYSWLTQQDLIETGVTMPDTEKVIDLVRGTRDADVAALYKQQEDGAWRVSLR
                     SKGPVSVGSLARERGGGGHELAAGFTAAELESSVEDLLHALRS"
     gene            complement(1523..1873)
                     /gene="rbfA"
                     /locus_tag="VNP73_04115"
     CDS             complement(1523..1873)
                     /gene="rbfA"
                     /locus_tag="VNP73_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWL65140.1"
                     /translation="MTQRMEKVQKRAKQVLGEAIQDLKDPRIGFATITAVRVTPDLRH
                     ARVLVSVLGTEDEQTATMKGLESAGSHLRTELGRHIRMKYLPELTFELDTRAAEAQRL
                     EILLKKVHDAEETD"
     gene            complement(1870..2178)
                     /locus_tag="VNP73_04120"
     CDS             complement(1870..2178)
                     /locus_tag="VNP73_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWL65141.1"
                     /translation="MFIGIGRFDLFIAASGSLKDKRQVLRSVTQSVAKRFNVSVAEVD
                     FQDLWQRAAIGVSCVSESAHHCRKVMQEVEKAIGRSALDGAEIVARSIEVISLEDLES
                     "
     gene            complement(2200..>4287)
                     /gene="infB"
                     /locus_tag="VNP73_04125"
     CDS             complement(2200..>4287)
                     /gene="infB"
                     /locus_tag="VNP73_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWL65142.1"
                     /translation="EEVKSHASTIDEDLAARVKADLSNGAAPKAAAPKKTTAKKAPEK
                     PAAAKPAPKPASKPAAAAKAPPAPAPPKEEPKAAPPPPAPPAAPPPAPAQEQKPAPAA
                     PPAPVAPAADAVKVHRGITVKELSDKIGRSPADIVKVLLGLGEMVTVTQSMSDEAVML
                     VGEELEVALQVIDPEEEEALEEAAAELEEDEGKLEPRPPVVTVMGHVDHGKTAILDAI
                     RKTNVAGGEAGGITQHIGAYRVAVAGRQVTFIDTPGHAAFTAMRARGAQVTDIAVLVV
                     AADDGVMPQTVEAIDHARAAGVPIVVAVNKIDKPEADPTRVRQQLSDHGLMPEEWGGE
                     TVFVDVSAKQRQNLDQLLEMILLTGDIQLDLKANPDAHARGVVIEAHLDKGRGPVATV
                     LVKRGTLYQGDALVGGAAWGRVRAMLDESGNQVEEARPGQPVVLLGWQSLPQAGDEFR
                     SLDDEREARSIAQEREQHRRAAELVAQRSVSLQSLLAATREGEVPELNLLLKADTQGS
                     VEALDDQLGKLDQSLVKLNILRKGAGAITENDITLAQASSAIVIGFNVIENAQARALA
                     EEGGVDVRTYRVIYQAVQDIEDAAKGLLGPEIREVPLGQAEVRATFRVPRLGLVAGCM
                     IVQGVIRRNSKARLVRDGVVIYETTVSSLRRFKDDVREVATGFECGIGLEGYQDIKEG
                     DLIQAFDVQEVAR"
BASE COUNT          704 a         1438 c         1323 g          822 t
ORIGIN      
        1 gtcatgtgcg cgccgcaccc gagtttctct ccgatatcgg cgatgagcgt tcggacatac
       61 gtcccaccgg agcagcgcac gtcaaactcg aacagaggtg ggtcgaacga gatgacttcg
      121 gccgaatgga tcgtcaccga gcgggggggc cgctccacct cctcgcctcg ccgggctttc
      181 ttgtagaggc gctccccgcc gatcttcacg gccgagacca tcgggggtac ctgttcgatc
      241 tcgccgacga acgatccgac cgcggtctct agttcggtgc gcccaatgtc aacgggacga
      301 acatctatct cgtccccgct cgcatccaga gtcgtcgtcg ttatcccgag cttcccttgc
      361 gctcgatacg acttgggtcc ggcttgcgcg tatgacagga tccgcgtcgc acgtccgaga
      421 ccgaggatca acactccggt tgcctcgggg tcgagcgttc cggcatggcc gacccgcttc
      481 gtcttgaaca gctttcggat cctgtcgacg acatcgtggg atgtgatccc cgccggcttg
      541 tcgacgacaa gaactccgtc cgtcatgagc ggagcgcgtg caggagatcc tcgacggagg
      601 actcgagctc ggcagccgtg aaccccgctg ccagttcgtg gcctccccca ccacgctcgc
      661 gcgccagcga tccaacgctc accgggccct tggacctcag gctgacgcgc caggcgccat
      721 cctcttgctg tttgtagagc gcggcgacgt ccgcatcccg cgtccctcgc accagatcga
      781 tgaccttctc ggtgtcgggc atggtgaccc ccgtctcgat cagatcttgc tgcgtcagcc
      841 acgagtagac gaaccgctcg gcaccatgaa ggaccgctct atccagaaca cgtccgacga
      901 gtttcaggta accgaatggt gccgactcga agacctcgag tgcgatcgca ggcgcatcca
      961 ccccactggt cagcaagtcg gccgccatcc gtaacgtgtc gggcgacgag ttcgagtacg
     1021 agaaccgacc cgtgtccgtg acgatgcccg tgtagagaca ggtcgcgatc gcgacatcga
     1081 tctcgatgcc caggtccaag agcagccgcc cgacgagctc ggcggtcgaa gatgcctgtg
     1141 tcgcgacgat gttgtacgtc ccgaagtccg agttcccggg atggtgatcg atattgatca
     1201 ggaccggcgc cttcgatgct ttctcttcga ggccgccgag ccggtcgctg cctccgcaat
     1261 caagggcaag gaagacatcg cagtccggca cctcctcggg ggccacgaac agatcgttgc
     1321 cggggaggaa gcgatacccg gacggcactt cggcccccgg ggtcccccac gagggccaca
     1381 cctctttccc gaggcgcttg agcgcgatgg ccgacgcgaa cagagagccg agagcgtctc
     1441 cgtcggggtt gacgtgacac gcaacggcga cgcggttcgc cccctgaagg gcgtccaccg
     1501 cacctttcca gtcgcgctcc atctagtcag tctcctcagc gtcgtgaacc ttcttcagaa
     1561 ggatctccaa acgctgagct tcggcggcac gggtgtccag ctcgaaggtc agctccggca
     1621 ggtacttcat ccggatgtgc cgtcccagct cggtgcgtag gtgcgaaccc gcgctctcga
     1681 gacctttcat cgtcgcggtt tgctcgtcct cggttcccag gacactcacg agtacgcgcg
     1741 catgtcggag gtccggcgtg acccgcaccg cggtaatggt cgcgaaaccg atcctggggt
     1801 ccttgaggtc ttggatcgcc tcgcccaaca cctgcttcgc tcgcttctgg accttctcca
     1861 tcctctgggt catgactcga gatcctctag tgagatgact tcaatcgacc tggccacgat
     1921 ctcggctccg tcgagggcgc tgcgtccgat cgctttctcc acctcctgca tgaccttgcg
     1981 acagtgatgg gccgactccg agacgcacga gaccccgatg gccgcacgct gccacagatc
     2041 ttggaaatcc acctcggcca ccgaaacgtt gaacctcttg gccacggatt gtgtgaccga
     2101 acgaagaacc tgccgcttgt ctttcagcga accgctcgct gcgatgaaga ggtcgaaccg
     2161 cccgatccct atgaacacta gcgggccact cgaccgaagt tagcgcgcga cctcttgcac
     2221 atcgaaggct tggatcagat cgccttcctt gatgtcctgg tatccctcca ggccgatacc
     2281 gcattcgaaa ccggtcgcaa cctcgcgcac gtcgtccttg aagcgtctca gggaagacac
     2341 ggtcgtctcg tagatcacga caccgtcgcg cacgagacgg gctttcgagt tccgtcggat
     2401 gacgccttga acgatcatgc aacccgccac caagccgaga cgaggaaccc tgaacgtcgc
     2461 gcgcacctcg gcctggccga ggggcacctc gcggatctcg ggaccgagca gacccttggc
     2521 tgcatcttcg atgtcctgca ccgcctggta gatcactcgg taggtgcgaa cgtcgacccc
     2581 gccctcttct gcgagagcac gagcttgcgc gttctcgatc acgttgaaac cgatcacgat
     2641 cgcgctcgaa gcttgggcga gcgtgatgtc gttctctgtg atcgcgccgg cgcctttgcg
     2701 gaggatgttc agtttgacca gagactggtc gagcttcccg agctgatcgt ccagtgcttc
     2761 gaccgaaccc tgggtgtcgg ccttcagcag caggttgagc tccggcacct ctccttcgcg
     2821 cgtggctgcg agaagggatt gcaacgaaac gctgcgctgg gccacgagct cggcggcgcg
     2881 gcggtgctgc tcgcgctctt gggcgatgct gcgagcctca cgctcgtcgt cgagggaacg
     2941 gaactcgtct cccgcttgcg gaagcgattg ccatcccaac agaacgacag gctgacccgg
     3001 ccgggcctcc tcgacctgat tgccgctctc gtcgagcatc gcgcgcacac ggccccacgc
     3061 ggccccccca acgagtgcat cgccctggta cagcgttccc ctcttgacca gaacggtggc
     3121 aacggggcct cggcccttgt cgaggtgggc ctcgatcacg accccgcgtg catgggcatc
     3181 cgggttcgct ttgaggtcga gctggatatc gcccgtcaga aggatcatct cgaggagctg
     3241 gtcgaggttc tgacgctgct tcgccgagac gtcgacgaag accgtctcgc ctccccactc
     3301 ctcaggcatc aatccgtgat cggagagctg ctggcggacc cgagttggat cggcctcggg
     3361 cttgtcgatc ttgttgaccg caacgactat cggaaccccg gctgcgcgcg cgtggtcgat
     3421 cgcttccacc gtctgcggca tgacaccgtc gtcggcggca acgacaagca ctgcgatgtc
     3481 ggttacctga gcaccccggg cgcgcatcgc ggtgaacgct gcgtgaccgg gtgtgtcgat
     3541 gaacgtcacc tgtcggccgg cgacggccac ccggtaagca ccgatgtgct gggttatccc
     3601 accggcctcg ccgccggcga cgttcgtttt acggatcgcg tccaggatcg cggtcttgcc
     3661 gtgatcgacg tgacccatga ccgtgacgac ggggggccgg ggctcgagct ttccttcgtc
     3721 ttcctcgagt tccgcggccg cttcctcgag agcttcctct tcctccgggt cgatgacctg
     3781 cagtgccact tcgagctctt cgccgacgag catcacggcc tcgtccgaca tcgattgggt
     3841 aacggtcacc atctcaccaa gcccgagcag gaccttgacg atgtcggccg gcgaccgtcc
     3901 gatcttgtcg gaaagctcct tgacggtgat cccccgatgg accttgacgg catcggcggc
     3961 cggcgcaacg ggcgcaggtg gcgcggcggg agccggcttc tgttcttgtg cgggagccgg
     4021 cggcggcgcg gcgggcggag ccgggggcgg aggggcggcc ttcggttctt ccttaggagg
     4081 ggcaggtgcc ggaggagcct ttgcagccgc tgcgggtttc gacgcgggtt tcggcgcggg
     4141 cttagccgcg gccggcttct cgggagcctt cttggcggtg gtcttcttgg gtgccgcagc
     4201 cttgggtgcc gctccgttgg acaggtccgc cttcacccgt gcagcgagat cctcgtcgat
     4261 cgtggacgcg tgggacttga cctcctc
//
LOCUS       DATMED010000163         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_499932, whole genome shotgun sequence.
ACCESSION   DATMED010000163 DATMED010000000
VERSION     DATMED010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499932"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VNP73_04130"
     CDS             complement(<1..228)
                     /locus_tag="VNP73_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65143.1"
                     /translation="MEGTAVSTDTFPQVDEDYVVTTLSKLAKVPTVVPMGWETFMEPD
                     DPKLVHYVQEVIRPELDRLAVRNILDVPRNNL"
     gene            complement(269..985)
                     /locus_tag="VNP73_04135"
     CDS             complement(269..985)
                     /locus_tag="VNP73_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWL65144.1"
                     /translation="MSFQPVSRNPIYLQVAEQIRAAIYSGQWPQGQPLPPERELSEMF
                     GVSRASIREALRVLEAEELISTDGKSPRTVKGGLQAGALTGALMHLLQLQRVELDDLV
                     DFRCLIEGAALRQAAARQDERYLDEARQVLEEMKATEMSAEETDAADIRFHVALTAAS
                     GSEIMRLVMIGIRQAAAKHFMAALSSHADPHGIIASLAHEHEEILRAVQSRDGERAAA
                     LVDSHITRFYREEVRPAQAE"
     gene            1119..2132
                     /locus_tag="VNP73_04140"
     CDS             1119..2132
                     /locus_tag="VNP73_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWL65145.1"
                     /translation="MGETVQDVEVGLSLPNRGVLFGLSPQVLLDVADIAEESGAFGSV
                     WVGDNFLSKPRLEAIVTLAGIAARTKRLKLGTICLATFPMRHPLPLAIQWASLDVLSG
                     GRTILAVCNGGSAKDGPAFALELETMGVASKERVGRVEEGIEILRRFWSGDPVDFAGE
                     FYTFRGVRVEPRPVQNRIPIVIAANPHPSQGDEVEERILRRVARLGDGWQTDGLPPEL
                     FRKRWSRIRQLAREEGRGSEVNHVSLHLMVNINDDAAAARRAAVGFLTHYYGAGTISE
                     EKLSSWLAFGSPAAVVEKIEEFIEAGCTTPVLRFVDADQVGQMRRCIEEVLPHLSKTT
                     SVA"
     gene            2142..>3343
                     /locus_tag="VNP73_04145"
     CDS             2142..>3343
                     /locus_tag="VNP73_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65146.1"
                     /translation="MTRNPRPTIAVFAGPTATILNTPPLVTSEKARIAYGLASDDEGN
                     PGRGLVDGLRPQRLAAPVTVYVEQFSAHPLESDAAHLYAPPDGYIADGTFRKARQSPS
                     DTPVYEVTLEPEDGLYLLPYFGRQADGTAWGGACQTTGSEFSSCRQTFYPDASRLFEE
                     IDRLGVGDDGRAGQLSSKAEYHFYRAVPPGGYTRGVPAAERTDIGDGDIGQERLGVDF
                     FPYLPARREPGRRELAQVTNTVAGALRANEYAGALWLEGSPNAEETCYWLSLLIDTTT
                     AIVATTAADSPHGAVRGSGDRNIIDAVEYIISSVWQDDVGRDLVGPTLIQDTQIFTAR
                     DAQKGGARAGGFVATGGHGGLVGAVGHGGEPVITFFPNRMFTRASQLNMTRLGSHARG
                     VRRTPRGG"
BASE COUNT          591 a         1047 c         1098 g          607 t
ORIGIN      
        1 gaggttgttg cgtgggacgt ccaggatgtt gcggacggcc agccggtcga gctcgggacg
       61 gataacctct tggacgtaat ggacgagctt tgggtcgtcc ggctccatga acgtttccca
      121 tcccatcggc accacggtcg gcaccttggc cagtttcgat aacgtggtga cgacgtagtc
      181 ctcatcgacc tgcggaaacg tgtccgtcga cactgcggtg ccctccaatt gcctcagcga
      241 ctcccttcaa tgtcccgacc ggtaggcctc attcggcctg tgccggacgc acttcttcgc
      301 gatagaaccg cgtgatgtga ctgtccacca gtgcagcggc ccgttcgccg tctcgagatt
      361 ggacggcgcg aaggatctcc tcgtgctcgt gggccagcga cgcgatgatg ccgtgagggt
      421 cggcgtggga actcagagcc gccatgaagt gtttcgccgc cgcctggcgg atgccgatca
      481 tcaccagccg catgatttcg ctgccggaag cagcggtgag tgcaacgtgg aatcggatat
      541 cggctgcgtc cgtttcctcg gcgctcatct cggtcgcctt catctcctcg agcacctgcc
      601 gagcctcgtc caggtaccgc tcgtcctggc gagccgccgc ttgacgcagc gcagcgccct
      661 cgatgaggca ccgaaagtcg acgaggtcgt cgagctcgac ccgttgcagc tgaagcaagt
      721 gcatcaaggc accggtgagc gcgcctgcct gaagaccacc cttgacggtt cgcgggctct
      781 tcccgtcagt cgagatcagc tcctccgctt ccaggacacg gagagcttct cgaatcgagg
      841 cgcggctgac gccgaacatc tcggagagct ctcgctcggg tgggaggggt tgtccctgtg
      901 gccactgacc gctgtagatc gccgctcgga tctgttcggc gacctgcagg tagatcgggt
      961 tccttgagac tggctggaac gacataccgc tgatctcggt gtgcttgact gcttgtccga
     1021 caatactacg atagtccagt cgggcaacta tttcacgccc tggagttccc ggtcacgcgg
     1081 tttcggaagg gcgaccaaca acattgatgg agtcggccgt gggcgaaaca gtccaggacg
     1141 ttgaagtcgg tctcagcctg cccaaccggg gcgtgttgtt cgggttgtca ccacaggttc
     1201 tcctcgacgt cgcggatatt gccgaggagt caggggcatt cggatcggta tgggttggcg
     1261 acaacttcct gtcgaagccg cggctcgagg cgatcgtcac cctcgcgggc atcgcagcca
     1321 ggacgaagcg gctcaagctc ggcacgatct gcctggcgac gtttccgatg cgtcatccgc
     1381 tgccgttagc catccaatgg gcgagtcttg acgtcctctc aggtggtcga acgattctcg
     1441 ccgtatgcaa cggcggctcg gcgaaggatg gtcccgcgtt tgccctcgaa ctcgagacca
     1501 tgggagtcgc gagcaaggaa cgtgtcggtc gggtggagga gggcatcgag atccttcgtc
     1561 ggttctggag cggtgatccg gtcgacttcg cgggcgagtt ctacaccttc cgtggagtcc
     1621 gggttgagcc ccgccccgtt cagaaccgca tcccgatcgt catcgcggcc aacccgcatc
     1681 cctcacaggg tgacgaggtg gaggagagaa ttctccggcg cgtcgcgagg ttgggcgatg
     1741 gatggcagac cgatggcctt ccgccggagc tcttccggaa gcgatggagt cgcatccggc
     1801 agttggcaag ggaggaaggc cgtggtagcg aggtgaacca cgtcagcctc cacctgatgg
     1861 tgaacatcaa cgacgacgcg gcggcggcgc ggcgggcagc ggtcgggttc ctcacccact
     1921 actacggagc tggcacgatc agcgaggaga agctgtcctc gtggctcgcc tttggatccc
     1981 ctgcggccgt cgttgagaag atcgaggagt tcatcgaagc cggatgcaca acgcccgttc
     2041 tccgcttcgt cgacgctgac caggtaggac agatgcgacg gtgcatcgag gaggtgctac
     2101 cccatctgtc gaagaccacg tcagtcgcgt agatgtgagc cgtgactcgg aacccccggc
     2161 cgacgatcgc ggtgttcgcc gggccgaccg ccacgatcct gaacacgcca ccgctggtca
     2221 ccagcgagaa ggcgcggatc gcttacgggc tggcaagcga cgatgaaggg aacccaggga
     2281 gaggcttggt cgacggattg cgacctcaac ggctggccgc gccggtcacg gtctacgtcg
     2341 agcagttctc cgctcatcca ttggaaagtg acgccgcgca cctctacgcg ccccccgacg
     2401 gctacatagc cgacggcacg tttcggaagg cgcggcagag cccatccgac acgccggtat
     2461 acgaggtgac gctggaaccc gaggacggcc tgtacctgct tccgtacttt ggaaggcagg
     2521 cggacgggac cgcgtggggg ggagcctgcc aaaccacggg cagcgaattc agttcgtgcc
     2581 gtcaaacctt ctaccccgac gcgtcccggc tcttcgaaga gatcgaccgc ctcggggtgg
     2641 gcgacgacgg tcgggcgggc cagctctcat cgaaggctga ataccacttc taccgtgcgg
     2701 ttcctccggg cgggtacaca cgaggtgtgc ctgccgccga acgaaccgac atcggggacg
     2761 gcgatattgg tcaggaacgt ctcggcgtcg acttctttcc atacctaccg gcgcgtcgcg
     2821 agccgggccg gagggaactg gcgcaggtca cgaacaccgt cgcaggcgcc ctccgcgcga
     2881 atgagtacgc gggagccctc tggctggaag gcagtccgaa cgctgaggag acgtgctact
     2941 ggctgagctt gctgatcgac acgacgacgg cgatcgtcgc gaccacggcg gccgactctc
     3001 cccatggggc cgtacgcggc tccggagacc ggaacatcat cgatgccgtc gagtacatca
     3061 tctcgagcgt ctggcaggac gacgtcggcc gggacctcgt tggtcccaca ctcatccagg
     3121 acacgcaaat tttcaccgct cgcgatgccc agaagggcgg cgcgcgagcc ggaggcttcg
     3181 tcgctaccgg cgggcatggg ggactcgttg gggccgtcgg tcacggtggg gagccagtca
     3241 tcaccttctt cccgaaccgt atgttcacgc gcgcctcgca gctgaacatg acccggctcg
     3301 ggtcccatgc acgaggagtt cgccggactc ccagaggggg gat
//
LOCUS       DATMED010000164         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1661445, whole genome shotgun sequence.
ACCESSION   DATMED010000164 DATMED010000000
VERSION     DATMED010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661445"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VNP73_04150"
     CDS             <1..637
                     /locus_tag="VNP73_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65147.1"
                     /translation="DEGGPLCILLSPHGDETGVYRRVRGDLDAFGIGGVQAERRTDRA
                     FGKSLAAAWNKPLLSEDPDHGVVVPLIQGIPLQLRVVAACLKEWTGPEEGDVESVIAE
                     ARAFAGAVIELSVDHDIIVAASAHSAASLSPGAPLMERAEGHELEQEITKALETDLGM
                     LASIDVGLWKAAGACGVGPLTAFGVLFEGHTARASFREAPFGVGYLLAQTE"
     gene            634..1491
                     /gene="miaA"
                     /locus_tag="VNP73_04155"
     CDS             634..1491
                     /gene="miaA"
                     /locus_tag="VNP73_04155"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWL65148.1"
                     /translation="MKPRVAAIVGPTAAGKSALAVEVAERRDAEIVSVDSMQIYRGMD
                     IGTDKASSEMRERIPHHLLDVKDPGADVTVAEFQSLARACIEEISARGKLPLLVGGSG
                     LYFRAVVDELRFPPTEPAVRARLEEESEREGAEALHARLVELDPEAAAKIEPANVRRT
                     VRALEVIQITGRPFSENADWDRYESIYDLRPIGLAPDRDRLDERIEARVRQMLDSGLI
                     EEARSLEVKGMGRTARMALGYRQVLDHPEAGVEELHGAIARATKRFARRQESWFKADP
                     RIRWLPAGR"
     gene            complement(1474..>2635)
                     /locus_tag="VNP73_04160"
     CDS             complement(1474..>2635)
                     /locus_tag="VNP73_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL65149.1"
                     /translation="LPQIRETFSLTQAQASLWISVLYLGALPALLLSRRADRIGRRRL
                     LILTVWGYSIATALTAAATSIVLFALLQFTARVFLAAEVAIAWTMIAEELPANKRGLG
                     FGALAMLAALGTGLAAIVWGLVLAPLDLSWRWLYVAGLPVLALVIRLRRDLPESRRFE
                     AAQEQDRLTSRWTEIFQPPYRRWLLLACTALFLAELTTQAQVFVVDFMQTQRGLSPSA
                     ANLVLITAGALAIPVLIYAGAMSDHYGRKRVAIFFLIVSLAGPLLLFMVARSPIALFA
                     SLALTYAGSFGAWPTLGAYGAELFPTRLRALGGAGVGFAKIGGQFASFVAAAVLISTL
                     GSLSLAVAVLTIGPLLAVFATSALPETMGKELETVSGDDLIVPFAGELPTGR"
BASE COUNT          510 a          816 c          892 g          417 t
ORIGIN      
        1 cgacgaaggc ggtcctctct gcatcctgct gtcaccccac ggagatgaga cgggcgtcta
       61 caggcgcgta cgtggagacc ttgatgcctt cgggataggt ggcgtgcaag ccgaacggcg
      121 taccgatcgc gcgttcggca agtcgctcgc ggcggcgtgg aacaagccgt tgttgagcga
      181 agacccggat cacggcgtcg tcgtcccgtt gatccagggg atcccgttgc aattgcgcgt
      241 cgttgcggca tgcctcaagg agtggaccgg gccggaggaa ggtgacgtcg agtcggtcat
      301 cgcggaggca cgtgcgttcg cgggggcggt tatcgagttg tccgtagacc acgacatcat
      361 cgttgctgcg agcgcccact cggcagcatc gctgtcgcct ggtgcccccc tgatggagcg
      421 agctgagggt cacgagctcg aacaagagat cacgaaggcc ctcgagacgg atctcggcat
      481 gctggcgagc atcgacgtgg gtctctggaa agcagccggc gcgtgcggcg taggaccact
      541 cactgccttc ggcgtcctgt tcgagggtca caccgcgcgc gccagcttcc gggaagcccc
      601 tttcggagtg ggttacttgc tcgcacagac tgaatgaaac cgcgcgttgc cgcgatagtg
      661 ggtccgaccg cggccgggaa gtctgcgctt gccgtcgagg tggccgagcg acgcgacgca
      721 gagatcgtgt cggtcgattc gatgcagatc taccgaggga tggatatcgg caccgataag
      781 gcgtcctccg agatgcgtga acggatccca caccatctcc tcgacgtgaa ggaccccggg
      841 gccgacgtga ccgtcgctga gttccagagt ctggctcggg catgcatcga agagatctct
      901 gcacgcggca aacttccgct gctggtcgga ggctccgggt tgtacttccg ggcggtcgta
      961 gacgagcttc gattccctcc gacggaaccc gcggtcagag cccgactcga agaagagtcc
     1021 gagcgcgagg gggctgaggc tctgcatgcc cgcttggtcg agctggatcc ggaggccgcg
     1081 gcgaagatcg agccggcgaa cgtccgacgg accgttcgcg ccctcgaggt gatccagatc
     1141 acgggtcggc ctttctcgga gaacgcggat tgggatcgtt acgagagcat ctacgaccta
     1201 cgccccatcg gactcgctcc cgatcgcgat cggctggacg agcggataga ggcgcgtgtg
     1261 cgacagatgc tcgactcggg attgatcgag gaggcaaggt cgctcgaggt gaaaggcatg
     1321 ggacggacgg cccgcatggc gctcggctac cggcaggtgc tcgatcatcc cgaagcgggc
     1381 gttgaagagc tccatggtgc gatcgcacgc gctaccaaga gattcgcccg ccggcaggaa
     1441 tcgtggttca aagccgaccc gaggatccgc tggttaccgg ccggtcggta gctctcccgc
     1501 gaacggcacg atgagatcgt cgccggaaac ggtttcgagc tctttgccca tcgtttcggg
     1561 tagagcactc gttgcgaaca ccgctagtag agggccgatc gtcagcacgg ccacggccaa
     1621 cgacaggctt ccgagcgtcg agatcaacac tgcggccgcc acgaagctgg cgaactgacc
     1681 gccgatcttg gcgaatccaa ccccggcccc gccgagagcg cgcagtcgag tgggaaacag
     1741 ctccgccccg tacgcaccga gggtgggcca cgcgccgaac gaacccgcgt acgtcagagc
     1801 tagagacgcg aacaacgcga tcggcgagcg agcgaccatg aacaacaaca gtggtcccgc
     1861 gaggctgacg atcagaaaga agatcgcgac ccgtttgcgt ccgtagtggt cgctcatcgc
     1921 acctgcatag atcaggaccg ggatagcgag cgctccggcc gtgatcagca ccaggttcgc
     1981 ggccgaaggg ctcaatcccc gctgcgtttg catgaagtcg acgacgaata cctgcgcctg
     2041 ggtcgtgagc tctgccagaa aaagcgcggt gcatgcgagt agcaaccacc ggcggtaggg
     2101 gggctggaag atctcggtcc agcggctggt gagtcggtcc tgttcctggg ctgcttcgaa
     2161 cctgcggctc tccgggagat cgcgcctcag ccggatcacg agtgcgagaa cgggcaggcc
     2221 cgcaacgtag agccaacgcc acgacaggtc gagtggcgcg agcacgagcc cccacacgat
     2281 cgcggcgagg cccgttccca aggccgcgag catcgcgagc gccccgaacc cgagccctcg
     2341 cttgttcgca gggagttcct ccgcgatcat cgtccacgcg atcgcgacct cggcggccag
     2401 gaaaacacgc gccgtgaact gaaggagcgc gaagaggacg atcgaggttg ccgccgcagt
     2461 cagagcggtg gcgatcgaat agccccacac cgtcaagatc agcaaccgcc ttctgccgat
     2521 gcgatcggcc cggcgcgaca acaacaacgc cggcaacgcc ccgaggtaga gaacgcttat
     2581 ccacagcgag gcctgggctt gggtgagcga gaaggtttct cggatctgcg gcagg
//
LOCUS       DATMED010000165         4808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3200600, whole genome shotgun sequence.
ACCESSION   DATMED010000165 DATMED010000000
VERSION     DATMED010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3200600"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VNP73_04165"
     CDS             <1..554
                     /locus_tag="VNP73_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL65150.1"
                     /translation="MEMGMRHPDRVKAIACLCPAAAFSRRPALSLVKLLRPELGALAS
                     RLPRARILPSMRQLFADPACVEESWYEAAVDDFLHVWKSPRARMAFFAALRNIYLDEP
                     EGETGFWTRLHAMDTPSMFIYGKRDMLISHHFARKVRRTLPRSEVVVWSDCGHVPQIE
                     FPDRTVDALSGFFRRSVARRVSA"
     gene            complement(613..1749)
                     /locus_tag="VNP73_04170"
     CDS             complement(613..1749)
                     /locus_tag="VNP73_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL65151.1"
                     /translation="MNFELPDEIAQFREAAKDFARREIAPLVDEAEQNERTPMELFKK
                     AGEAGFVGIRYPEEIGGSGASVLAEVVQREEFGYVCSGIASALSVSSHLGTFPVFEFG
                     SDEQRKRYLEPALRGEKVSAFALTEPNAGSDVRGIQTKAVRQDGGWVLSGTKTFITNA
                     TIADFLSVVAYTDPSAGIDGMALFVVETANENIEVNRLKKMGNLSSEIAEVVFSDAFV
                     PDDALLGEERGAFKKVQQTLNIGRVLVSGGALGVAQAALDSAVQYAKDRSAFGQTIGS
                     YQGVSFPLAKAHATIEAARLAVYKAAWLWDQGRNPVIETSTAKLLAGEAGVDVTDKCI
                     RTFGGYGYMREYPIERFYRDARYFTIVEGTQEIHQRVISKQLGL"
     gene            1816..2664
                     /locus_tag="VNP73_04175"
     CDS             1816..2664
                     /locus_tag="VNP73_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65152.1"
                     /translation="MDPASIVVADRSQRAKLRFTGEQRAWFLHQILTQAFEDVQPGEA
                     RDAAMITAHGRMTAYLETLATQDAIYAHFESQLGESLPEQIRNYVFATRVEIDDVSDE
                     FGLVLIAGDGWRELAEAQVPVPRMHPTSSLGIPAGYVWLDRSYVADVLTALEKEGAAR
                     ATEKELEEIRISNGVPRWGFEMDSKTFPQEVGIDEAAVHYDKGCYLGQEAMAKISFRG
                     KVNRRLAKVEIDGQAERGDELVLDEKRVGMITSVAGSKGLAVVSHTVEPGTLLYAGDN
                     AVKVID"
     gene            2664..3263
                     /locus_tag="VNP73_04180"
     CDS             2664..3263
                     /locus_tag="VNP73_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL65153.1"
                     /translation="MTHHYEGPDLIVRKIEVGTMENNVYVLECPETHDAFVVDGCFET
                     EKILEGADGANVVGILQTHGHMDHVQALPELKERLGVPVHAHTGDDYPIEVDVQLNDG
                     DELKLGNRTVSVLHTPGHTPGGTCFLIGKHLISGDTLFPGGPGNTWGNKDAFEQIIGS
                     IESKLFVLPDETAVYPGHGNDTKIGAEKPHLQEWIDRGW"
     gene            3276..3746
                     /locus_tag="VNP73_04185"
     CDS             3276..3746
                     /locus_tag="VNP73_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65154.1"
                     /translation="MSEDNGGLTDSIARLPQRVTALFELITTLDQRVLAALDSVEAMR
                     KSIEPLEGVGASGDELVADLRGLMTRLEERANRDMDEIKELLMTKLGELDLGDLGPRV
                     DRLESAVLNIERATVHLDEAFQGGLEILPDFVTKRLRTEGRKAAPTPESGPPKI"
     gene            3753..4721
                     /locus_tag="VNP73_04190"
     CDS             3753..4721
                     /locus_tag="VNP73_04190"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWL65155.1"
                     /translation="MFTCPRDRVELNRTAGEAGVQWACATCGGRAVGIGPLRRTLTKE
                     FVNSLWASAVERDDIGERPCPVCEQPMLRSRVAPIDLDVCKRCQLVWFDPPEYETAPA
                     RPVEPKEQLTDDQLEAIGRLQAEEIAREWRKRPDSELSAQDLMMVPAALGLPLEEEVT
                     VVTRLPWVTWILAAVLFLVGIFFNAGLYHLLTNLYFLVVFGDNVEDFLGRLNYALLIA
                     VAGAAGAGVHWLFSPDPLTPLVGASAGVSGIVVFYGLRFPKAKLRYFRLFKWFSMPAS
                     TAVLFWAISQVIGSRAGLLPSPAVSVFAHLGGAAVGLWFWFMWRDD"
BASE COUNT          873 a         1502 c         1518 g          915 t
ORIGIN      
        1 cgatggagat ggggatgcgc catcccgacc gcgtcaaagc gatcgcgtgt ctgtgtccgg
       61 cggccgcgtt ctcgcgccgg cccgccctgt ccctggtcaa acttcttcga cccgagctgg
      121 gagcgctcgc cagtcggttg ccgcgtgccc ggatccttcc gtcgatgcgg cagctgttcg
      181 ccgatccggc gtgcgttgaa gaaagttggt acgaagcggc ggtcgacgat ttcctccacg
      241 tgtggaagag cccacgtgcg cgcatggcgt tctttgcggc gttgcggaac atctacctgg
      301 acgagcccga aggtgagacc ggtttctgga cccggctcca tgcgatggac acaccgtcga
      361 tgttcatcta cggcaagcgc gacatgctca tcagtcacca cttcgcgcgc aaggttcgga
      421 gaacgcttcc cagatcggaa gtcgtcgtgt ggagcgactg cgggcacgtt cctcagatcg
      481 agttccccga ccgcaccgtc gacgcactgt cgggtttctt ccgcaggtcg gtagcgcgcc
      541 gcgtctctgc ctagttcatt ctcctcagca aaatgtcgct atccgcaaca tttctctgaa
      601 cagaacgcga cctcagaggc ccaactgctt cgagatcacc cgttggtgga tctcttgggt
      661 tccttcgacg atcgtgaagt agcgcgcgtc ccggtagaag cgctcgatcg ggtactcacg
      721 catatagccg tagccgccga acgtccggat gcacttgtcg gtcacgtcca ctcccgcctc
      781 tccggccagc agcttggcgg tcgacgtctc gataacgggg ttccggcctt gatcccagag
      841 ccacgcggcc ttgtagacgg cgagtcgcgc cgcctcgatc gttgcgtgcg ctttcgcgag
      901 cgggaacgac acgccttggt agctgccgat cgtctgtccg aacgcgctgc gatcctttgc
      961 gtactgaacc gccgagtcga gtgccgcctg cgcgacccct agtgctccgc ccgagacgag
     1021 cacgcgtccg atgttcaaag tctgctgcac cttcttgaaa gcgccacgtt cttcgcccag
     1081 gagcgcgtcg tcgggcacga acgcatccga gaacacaacc tcggcgatct cagatgacag
     1141 gttgcccatc ttcttgaggc ggttgacctc gatgttctcg ttggccgtct ccaccacgaa
     1201 caacgccatc ccgtcgatcc cggccgaggg gtcggtgtag gccacgacac tgaggaaatc
     1261 ggcgatcgtc gcattcgtga tgaaggtctt ggtcccactg aggacccagc ccccgtcctg
     1321 tcgcaccgcc ttcgtctgga taccgcgcac gtcggaaccg gcgttcggtt ccgtgagtgc
     1381 gaacgcgctg accttctctc ctcttagagc cggctcgagg tagcgcttgc gctgttcgtc
     1441 gcttccgaac tcgaacacag gaaaggtgcc gaggtgcgag gacaccgaga gtgccgaggc
     1501 gatcccggaa cagacgtaac cgaattcctc tcgttggacg acttcggcca gcacgctcgc
     1561 cccggatccg ccgatctctt ccgggtagcg gatcccgacg aacccggcct ccccggcctt
     1621 cttgaacagc tccatcgggg tgcgttcgtt ctgctccgcc tcgtccacga gcggagcgat
     1681 ctcccgccga gcgaagtctt tcgcggcctc gcgaaattgg gcgatctcgt ctggaagctc
     1741 gaagttcacg cctgagtgtt taccgcagac ggtgaggcgg tgcgtccatg cgtccccggg
     1801 gggcaagata gaggtgtgga tccagcgtcg atagtcgtcg ccgatcgctc gcagcgggcg
     1861 aagcttcgct tcaccgggga gcagcgcgcc tggttcctgc atcagatcct gacccaggcc
     1921 ttcgaggatg tgcagccggg tgaggcgaga gacgccgcga tgatcaccgc tcacgggcgg
     1981 atgacggcgt acctcgaaac gctggccact caggatgcga tctacgcgca tttcgagagt
     2041 cagctcggcg agagccttcc cgagcagatc cgcaactacg tcttcgccac gcgtgtcgag
     2101 atcgacgatg tatccgacga attcggactc gtgttgatcg ctggggatgg atggcgcgag
     2161 cttgccgaag ctcaggtacc cgtccctcgc atgcatccga cgagttcgct cggcatcccc
     2221 gcgggatacg tgtggctgga tcgctcctac gtcgcagacg ttttgacggc attagagaag
     2281 gaaggtgcag cgagggcgac ggaaaaggaa ctcgaagaga tccgcatctc taacggcgtt
     2341 ccgcgctggg ggttcgagat ggactcgaag acgttccctc aggaggtcgg catcgatgag
     2401 gcggcggtcc actacgacaa gggctgctac ctcggtcaag aggcgatggc caagatcagt
     2461 ttccgcggca aggtgaaccg gcggttggcc aaagtcgaga tcgacgggca ggcggagcgc
     2521 ggggacgagc tcgttctcga cgagaagagg gtcgggatga tcacgagcgt cgcgggctcg
     2581 aaagggctcg cagtcgtatc ccatacggtt gagcccggaa cgctgctgta cgccggcgac
     2641 aacgctgtga aggtgatcga ctgatgacac accactacga aggtccggac ctcatcgttc
     2701 ggaagatcga agtcgggacg atggagaaca acgtgtacgt gctcgagtgt cccgagactc
     2761 acgacgcatt cgtcgtcgac ggatgtttcg agacggagaa gatcctcgag ggtgcagacg
     2821 gagcgaacgt ggtcgggatc ctccagacgc acggacacat ggatcacgtc caggctctgc
     2881 ccgagctcaa agaacgtttg ggcgtcccgg ttcacgcgca caccggcgac gattatccga
     2941 tcgaggtcga cgtccagctg aacgatggcg acgagttgaa actcggcaat aggacggtgt
     3001 cggttctcca cacgcccggc cacacgcccg gcggcacctg ctttctcatc ggcaagcacc
     3061 tgatctccgg tgacacgtta ttcccggggg ggccggggaa cacgtggggc aacaaggacg
     3121 cgttcgagca gataatcggc tcgatcgaga gcaagttgtt cgtccttccc gacgagacgg
     3181 ccgtctaccc cggacacggg aacgacacca agatcggtgc cgagaaacca catctgcagg
     3241 agtggatcga cagaggttgg tagcgttcca ccaccatgtc ggaggacaac ggcggcttaa
     3301 ctgattccat cgctcgtctg ccacaacgcg taaccgcgct tttcgaactg atcacgaccc
     3361 tcgatcagcg agtcttggcg gccctcgact cggtcgaggc gatgcgcaag tccatcgaac
     3421 cactcgaagg cgtcggcgcg agcggggacg aactcgtcgc cgaccttcgt ggcttgatga
     3481 cccgtctcga agagcgagcc aaccgcgaca tggacgagat caaggagctg ctgatgacca
     3541 agctgggaga actcgacctt ggtgacctcg gcccccgcgt cgaccgcctc gaatccgccg
     3601 ttctgaacat cgagagagca acggtccacc tcgacgaggc gttccaaggc ggcctcgaga
     3661 tcctgcccga cttcgtcact aagaggctcc gaaccgaggg gcggaaggcg gcgcccaccc
     3721 ccgaaagcgg tcctccgaag atctaagcgc ggatgttcac ttgcccgcgc gaccgcgtcg
     3781 aactgaaccg caccgcgggc gaagccggcg tccagtgggc gtgcgctacg tgcggtggcc
     3841 gagccgtcgg catcggacct ctgcgtcgca ccctgaccaa ggaattcgtg aacagcttgt
     3901 gggcttctgc ggtcgagcgc gacgacatag gggagagacc gtgtcccgtg tgcgagcaac
     3961 cgatgttgcg gagccgagta gcaccgatcg acctcgacgt ttgcaagcga tgtcagctcg
     4021 tgtggttcga ccccccggag tacgagacgg ctccagcacg gccggtcgaa ccgaaggagc
     4081 agttgaccga cgaccaactc gaagcgatcg gaaggttgca agcggaagag atcgcccgcg
     4141 aatggcgcaa gcgacccgat tcagagttgt cggcgcagga cttgatgatg gtcccggccg
     4201 ccctcggtct tcctctggag gaggaagtga cggtggtgac caggttgccc tgggtgacct
     4261 ggatcctggc cgcggtgctc ttcctcgttg ggatcttctt caacgcaggg ctgtatcacc
     4321 tgctcaccaa cctctacttc ctggtggtct tcggtgacaa cgtcgaggac ttcctggggc
     4381 gcctcaacta cgcacttttg atcgcggtgg ccggagcggc gggggccggg gtgcattggt
     4441 tgttctcgcc cgacccgctg acgcctctcg tcggtgcgag cgcaggcgtc tcggggatcg
     4501 tcgtcttcta cggactgcgt ttcccgaagg cgaagctgcg ctacttccgc ctcttcaagt
     4561 ggttctccat gccggcctcg actgcggtcc tgttctgggc catctcgcag gtgatcggtt
     4621 cgcgcgccgg actattgccc tccccggccg tttctgtctt cgcccacctc ggcggtgccg
     4681 ccgttgggtt gtggttctgg ttcatgtggc gcgacgacta gtagtggccc accatcagcc
     4741 gagaccttct ggttcgaagt ggatcgtgac ggacttcacg ttcggatgcg cgctcaggat
     4801 cgacttct
//
LOCUS       DATMED010000166         9659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3575024, whole genome shotgun sequence.
ACCESSION   DATMED010000166 DATMED010000000
VERSION     DATMED010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575024"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VNP73_04195"
     CDS             <1..660
                     /locus_tag="VNP73_04195"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWL65156.1"
                     /translation="LDPEEPAKIAEAVRLMGLKFAVVTGVARDDLDDGGSAHWAATVR
                     KIRAEMPDCGIEVLIPDFKGRKEHPRESLACVIEAGPDVLAHNLETVRRMHPSIRPGF
                     GFEASLELLSWAKELRAEQVTKSNLIVGMGETEEEVFDSMRDLRATGCDVLTIGQYLQ
                     PSVSWHLPVARWVHPDEFARYKAYGENELGFAWVESGPLVRSSYHAGKQYRSASARLA
                     G"
     gene            902..1426
                     /locus_tag="VNP73_04200"
     CDS             902..1426
                     /locus_tag="VNP73_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65157.1"
                     /translation="MLRKNGLVVGLVLVLVLAVPADAHRADLIRAGKRVGPIKLYETT
                     LRDARRWFGEPDERTVDERGCVKRVVRLLWRGELKVFAGRYKGETHPIAEVHVLDRSI
                     ISFEHGGLTIHTRRGIQVDEHQAKVRRAYPDARHETHRGHTHYIVEDPYDRVLVKVVD
                     REVVALEARPFEWC"
     gene            complement(1423..2604)
                     /locus_tag="VNP73_04205"
     CDS             complement(1423..2604)
                     /locus_tag="VNP73_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL65158.1"
                     /translation="MSAGLLGAALSIPPAHSRQGVTKGTITIGVHGPVTGASPLPSDS
                     LQQGADIYWRWRKHHDRPIKGRHVEVVFKNDNTNPSQAVAVCKEMVEQDNVFLLSGLL
                     NPEGKDQVQSCARYAASVGVPYVSLGQMKTGVAELPRYFAFSASWARQARLLADYMLD
                     RLGAGSENNGMVRFDTPNYTDTHDAFVSAMSKRKNELEYDRSVSRGAGATEAELVIGE
                     LKNAEIENVFVLVSPIWFLQLLKAADNQNYHPQWLGVGMTLSVTDSVVRVGCSGDAAV
                     RGTKVLSPLPAFDDHREFDDNYSKAMQEIYNDTGDMVTWLGWGTSRQLAKLLERAGRD
                     LSRRRFVRSSERARDIRTGILPALNFSPRDHFGGKGVHVLEARCTDERWHTKLRFKRG
                     F"
     gene            2662..3072
                     /locus_tag="VNP73_04210"
     CDS             2662..3072
                     /locus_tag="VNP73_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65159.1"
                     /translation="MLRIAPLLVALLLAAGCGNSDGPASTRSACPEPTPVTSKIPKDL
                     PFEDWGTVTKVERDERFLIVQVDSEQSVVELHPQIARALLGAGYEIVGADNEGFESEI
                     FFAPGKGLTGLFRLRQSPCKGRVIVTLLYEPESR"
     gene            3069..3875
                     /locus_tag="VNP73_04215"
     CDS             3069..3875
                     /locus_tag="VNP73_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWL65160.1"
                     /translation="MTRSVMRNVAVAGVAILFLAPLVFMFLGALRFPGLPPPDGFEVI
                     PRFVRWANFQSAFMVVSLLEQIMNSLLIAAVAVPVTVVIGSLAGYAIVASPPRVGKRL
                     AVISVIALLVPVTSLWIPRFVMFRWLGAIDTPWPLIAPALMATTPFFVLIFALTYSRI
                     PKQLLEAARLEGLSELSIWRRVVWPLGMPAAFAVAVLAFAFHWSNFMDALLYIYDEAN
                     FTVPLGLNALSSLEPPMRPIFLAASVVATIPVVIAFLAAYRAFFVRTLEV"
     gene            3875..5176
                     /locus_tag="VNP73_04220"
     CDS             3875..5176
                     /locus_tag="VNP73_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWL65161.1"
                     /translation="MKRGWGVVAIGLLLIAGACSQERPSDPQSTTGEVTLQVQGEVEE
                     VAVYDALVKSFELDVEGIDVNLVKLTDEDDILAKLTAQFASGNAPDVFTINFREFSQF
                     AAEGALEPAGPLLEAEGLDFGDYYPQPMQAFTYNDELQCMPQNLSSMVVFYNTQLFER
                     FNVPRPQPGWTWEDFRDTAIALNKEGAHGLGIDPHVIRLAPFIWSNGGELVDDPENPS
                     RFALDDPQSREAVEFIVSLVRDDGVVPSEKEVAAQALDERFAAGKLGMLLSSRREVPG
                     FREVQGLQWDAAAPPVAKEPSSILHTDGYCIAAGSENVDAAAKFVAYAMSKEGQTITA
                     LSGRTVPVLKSVAHSAAFLDPAQPPAHSEVWLEVIPHLRATPVIPTWPEIEALSEETI
                     IKMFYEPGFTIDDGIEELDRTTSDLFREGVERGLGGETGEG"
     gene            5173..6174
                     /locus_tag="VNP73_04225"
     CDS             5173..6174
                     /locus_tag="VNP73_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65162.1"
                     /translation="MSLSVESVAKRFGEVVALTGVDLSAADGELLVIVGPSGSGKTTL
                     LRCIAGLEEIDSGRVSVAGRDVTGADPGDRDVAMVFQEYALYPHLTVRANIAFGLKAR
                     KVDESSIERSCAEVVELLGLQNTTDRRPAELSGGERQRVALARAIVREPALFLMDEPL
                     ANLDAELRTHTRGEIKRLQKRIGTTMLYVTHDQIEAMTMGDSVAVMRAGTIEQIDSPL
                     NLYNHPANAFVARFIGHPPMNVFPAEVLSGEKRGAYAGIRPERLRLGDGPGSIAGEVE
                     LVEPIGGEVLVHLSVDSTRLIARIPGDEHPPIGTSVNVTFGADDVFWFEGPDGKTLA"
     gene            6189..7022
                     /locus_tag="VNP73_04230"
     CDS             6189..7022
                     /locus_tag="VNP73_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWL65163.1"
                     /translation="MALPYLAGLFLLVAVPALLAFALAFTEFPAVAAPRFNGVDNFIR
                     LFRDAGYATALWNSLIYLALSVPLRVVAAVGIALLMYRRSRGVGAARAAAYLPTVVPD
                     VAFGLLWLWLLNPLYGPIPGALQALGLPSPDWLIDPWSARFAVAIMSAFQIGEGFVIA
                     LAVRRLIPSSLYEAALLDGAGPWFTLTRLTLPLMAPAIALLALRDVVLALQANFVPAL
                     IVTDGGPRLATTFLSIYVYRQAFAYFRLGYASTISLTMFVLTAGIVYAQYRLARRWRL
                     L"
     gene            7141..9204
                     /locus_tag="VNP73_04235"
     CDS             7141..9204
                     /locus_tag="VNP73_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWL65164.1"
                     /translation="MSAFTARQKATLEALARVLLPEGHGLPGGDSAGAAAALDARAET
                     WDGRVLRGVKLLLDAWELSPLASRHRARFSMLGEADRVAWTMHCYRSKAAWRRLQVTA
                     LKQLLFLEWACSPVVEDALGYDYRCRKEGVPHGAARALVGDPDPGITEPEPADYHRTG
                     PAVASGAIPIALMSKRDFEGPQSRDLTTQAWPDLFDGQRENVDVVVVGSGAGGAVAAT
                     YLAEAGLNVLVLEEGQQVTADDFAGPMFDRLQRFCRGNGTTQVWGSPPIPLPLGKVVG
                     GTTVVNSGTCFRGPQRVLERWASEFGVEGARLDEMIEHYEPLEEFLNVRPVPWDLLGP
                     NGMAAHRGAVALGYSGGPLLRNITDCHGCGQCAFGCPTNAKQAMHVSFLPRAQMAGAR
                     IFSRCRVDTIVTERDGAAGVEATLLGPDGRSRGRLRVSATHVVVAAGAVYTPSLLKKS
                     GVPDPSGQTGRNLRIHPATGVGGAFDEDVKNWKGTLQSYYIDEFFDSHELMFEATTTV
                     PSIGAGALPGIGEQAMQELGRFRNLASLGFYVSDTSRGRVLTLRGGEAFPVYRMNALD
                     ARRMQIGLSIACEVMLEAGAKKVYPGIPGIDAIDARADLEQLRERRVRPEHLKLTAFH
                     PMGTARMGGDPSSSVVDPYGHHHAVERLWVVDSSTFPSCVAVNPQMTIMAFAKRTAEY
                     LARKF"
     gene            9221..>9659
                     /locus_tag="VNP73_04240"
     CDS             9221..>9659
                     /locus_tag="VNP73_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL65165.1"
                     /translation="MELSGSNAILTGASRGLGVYIADALALAGVNLALAARDQTGLDA
                     TADRVRTKGVKVATIPTDVGDEDSLRNLADQAEEQLGPIDILINNAGVEHYIDFHTAD
                     TKLIEKIMRVNILGPQVLTRFVLPGMVERKRGHIVNIASVAGKT"
BASE COUNT         1672 a         2989 c         3088 g         1910 t
ORIGIN      
        1 ctggatcccg aggaaccggc gaagatcgca gaggccgttc ggttgatggg cctgaagttc
       61 gcggttgtga ccggtgtggc ccgtgacgac ctcgacgacg gcggatcggc tcattgggct
      121 gcaaccgtcc gaaagatccg agccgagatg ccggactgcg gcatcgaggt cctgatcccc
      181 gacttcaaag gccggaagga gcatccgcgc gaatcactcg cctgcgtgat cgaggccggt
      241 cccgacgtcc ttgcccacaa cctcgagacc gttcgacgca tgcatccgag catcaggccg
      301 gggttcggct tcgaagcgag tctcgagctg ttgagctggg ctaaggagtt gcgtgccgaa
      361 caggtaacga agtcgaacct gatcgtcggc atgggggaga cggaagaaga ggttttcgac
      421 tcgatgcgcg acctgcgggc tacagggtgc gatgtgctaa cgatcggcca gtacctgcaa
      481 ccgagtgtgt catggcatct gccggtggcc cgctgggttc acccggacga gttcgcgcgc
      541 tacaaggcgt acggcgagaa cgagctggga ttcgcctggg tcgagtccgg ccccctcgtt
      601 cgctccagtt accacgccgg caagcaatac cggagcgcct ccgcccgcct cgcgggctaa
      661 gtctctttgc cctacaaaac gccctggtat cagcggagat ggttcagggc gctctttatt
      721 ctctggttcg ccagtcctct cttcgttcag cttttcgcag ccgaatacag accagcctgg
      781 atcatcttgg gtcctgctgc cgtggtcatg gggggcctca tctggttctt gaattggcgc
      841 aagtgaatga acctgttcgt tgccattctt ttctaagctc taggcgcaaa atgggggcca
      901 gatgttaaga aagaacggtt tggttgttgg gttggttctg gttctggttt tggccgttcc
      961 ggcggatgct caccgcgccg atctgatccg agcggggaag cgcgtcggtc ccatcaagct
     1021 ctacgagacg acactccgtg atgcaagacg atggttcggc gaacccgacg aacgtactgt
     1081 cgacgaacgt gggtgcgtga agagggtcgt tcgcttgctg tggcgcggcg agctgaaggt
     1141 gttcgcgggc cgctacaagg gagagaccca cccgatagca gaggtccacg ttctggaccg
     1201 ctcgatcatc tcgttcgagc acggcggcct cacgatccac actcgccgag ggatccaagt
     1261 ggacgagcat caagcaaagg tgcgccgcgc ctacccagac gctcggcacg agacccacag
     1321 agggcacacg cactacatcg tcgaagaccc ctacgaccgc gtgctggtga aggtcgtcga
     1381 tcgtgaggtt gtcgctctgg aggcgcggcc cttcgagtgg tgctagaagc cgcgcttgaa
     1441 gcgcagtttc gtatgccagc gttcgtcggt gcagcgggct tcgagcacgt gcacaccttt
     1501 gcccccgaag tgatcgcggg gcgagaagtt caacgccgga aggatcccgg ttcggatgtc
     1561 gcgtgcgcgt tcactacttc gaacgaagcg cctgcgcgat agatcccggc cggcccgttc
     1621 cagcaacttg gccagttgac gagacgttcc ccatcccagc cacgtcacca tgtctcccgt
     1681 gtcgttgtag atctcttgca tcgccttgga gtaattgtca tcgaattctc ggtgatcgtc
     1741 gaaagccggt aatggagaga gcaccttggt tccccggacc gctgcatcgc cgctacagcc
     1801 cacccggacg acggagtccg tcaccgacag agtcatcccg accccgagcc attgcgggtg
     1861 atagttctgg ttgtcggcag cctttagcaa ctgcaggaac cagatgggac tgaccaagac
     1921 aaagacgttt tcgatctcgg cattcttgag ctcccctatg acgagctcgg cctcggttgc
     1981 ccccgcccct ctggagacgc ttctgtcata ttccagctcg ttcttccgtt tggacatcgc
     2041 ggaaacgaat gcgtcgtggg tgtctgtgta attgggcgta tcgaaacgga ccatgccgtt
     2101 gttctcgctt ccggcaccca agcggtcgag catgtagtcg gcgagtaaac gcgcctggcg
     2161 cgcccacgac gccgagaaag cgaagtagcg ggggagctcg gcgacgccgg tcttcatctg
     2221 cccgagcgac acgtacggga ctccgaccga tgctgcataa cgcgcgcacg actggacctg
     2281 gtccttgccc tcgggattca acaagccgct caggagaaag acattgtcct gctcgaccat
     2341 ctccttgcaa acggcgacgg cctgggacgg gttggtgttg tcgttcttga agacgacttc
     2401 aacgtgcctg cctttgatcg gacggtcgtg atgtttacgc cagcgccaat agatgtcagc
     2461 gccttgttgg agcgagtcgg aaggcaaggg cgatgctccg gtcaccggac catgtacgcc
     2521 gatggtgatc gtgcccttgg tgacgccttg ccgcgaatgg gctggaggga tggacagggc
     2581 agcccccaga aggccagcgc tcacaattac agctatcgac cgtttcgtcg ccatatcccg
     2641 cgtctaactt aacgttcgtt gatgcttcgg atcgctccac tcctggttgc gctgcttcta
     2701 gcggctggat gtggtaactc agatggaccg gcatcgactc gctcggcgtg ccccgagcca
     2761 acccctgtca cctcgaagat ccccaaggac cttcctttcg aggactgggg gaccgtcacg
     2821 aaggtcgagc gggacgagcg gttcctcatc gtgcaggtgg attccgagca gagtgtggtc
     2881 gaactgcacc ctcagatcgc ccgagccctt ctcggggccg gctacgagat cgtgggcgca
     2941 gacaacgaag gattcgagtc ggaaatattc tttgcgccgg gcaaggggct tacaggattg
     3001 ttccgacttc gtcagagccc ctgcaaaggg cgggtgatcg tgacgctgtt gtacgaaccg
     3061 gagagccgat gacacgatcc gtgatgcgga acgtggccgt cgcgggagtc gcgatcctct
     3121 tccttgcacc gctggtcttc atgttcctcg gcgcactgcg tttccccggc cttcctcctc
     3181 cggatggctt tgaagtgatc ccgagattcg tcagatgggc gaacttccag tccgccttca
     3241 tggtcgtttc gctgctggag cagatcatga actcgctgtt gatcgcggcg gtcgcggttc
     3301 ccgtcaccgt ggtgatcgga tctttggccg gttacgccat cgtggcttcg ccaccacgcg
     3361 tcggaaagag gctcgcggtc atcagcgtca tcgcgctgct cgttcccgtc acgagtttgt
     3421 ggatcccccg tttcgtcatg ttccgttggt tgggcgcgat cgacacaccg tggccgttga
     3481 tagctcctgc cctcatggcg acgactccgt tcttcgtcct gatcttcgct ctgacttact
     3541 cccgcatccc caaacagcta ctcgaagccg ctcgactgga aggtctcagc gagctgtcga
     3601 tctggcggcg cgtcgtgtgg cccctgggga tgccggcggc gttcgcggtg gccgtccttg
     3661 ccttcgcctt ccactggtcg aatttcatgg atgccctgct gtacatctac gacgaggcga
     3721 acttcaccgt gccgctgggt ctcaacgcac tgtcgagcct cgagcccccc atgcgaccga
     3781 tcttcctcgc cgcctcggtc gtggctacca ttcccgtcgt tatcgctttc cttgctgctt
     3841 atagggcttt tttcgttcga accctggagg tgtgatgaaa aggggttggg gtgtcgtggc
     3901 catcggtttg ctcttgatcg cgggagcatg ctcgcaggaa cgaccttcgg acccccaatc
     3961 caccaccggc gaagtgacgc tccaggtgca gggtgaggtc gaagaggtcg cggtctacga
     4021 cgctctcgtg aagtcattcg agctggacgt cgaagggatc gacgtcaacc tcgtgaagct
     4081 caccgatgaa gacgacatcc tcgccaaact cacagcgcaa ttcgcatcgg gcaacgcgcc
     4141 cgacgtcttc acgatcaatt tccgtgagtt ttctcagttc gcggccgaag gtgctctcga
     4201 accggcggga ccgctactcg aagcagaggg ccttgacttc ggcgattact acccgcagcc
     4261 tatgcaggcc tttacgtaca atgacgagct ccaatgcatg ccgcagaacc tgtcctcgat
     4321 ggtcgtcttc tacaacaccc agttattcga gcgcttcaac gtccctcggc cacagcccgg
     4381 atggacgtgg gaggacttcc gcgacactgc catcgctctc aataaggaag gagcccacgg
     4441 tctcggcatc gatccacatg tgatccgtct ggctccgttc atctggtcga acggcggcga
     4501 gctcgtcgac gatccagaaa acccgagcag gttcgcgtta gacgacccac agtcgcgcga
     4561 ggcggttgag ttcatcgtct cgttggtgcg ggacgacggg gtcgttccca gcgagaagga
     4621 agttgcggcg caggctctcg atgaacgatt cgcggccggc aagctcggga tgcttctctc
     4681 ctcacgcagg gaggtgccgg ggttcaggga ggtgcagggg ctgcagtggg atgcggcggc
     4741 tccaccggtg gcgaaggaac catcgtccat cctccatacg gacggttact gcatcgccgc
     4801 cggatcagag aacgtcgatg ctgcggccaa attcgttgcg tacgcgatga gcaaggaagg
     4861 tcaaacgatc acggcgttgt cggggcggac cgttccggtc ctcaagtcgg tcgcgcactc
     4921 ggcggctttc ctcgatcccg cacaaccgcc ggcacactcc gaggtgtggt tggaagtcat
     4981 ccctcatcta cgagccacgc cggtcatccc tacatggcct gagatcgagg cattgtcgga
     5041 ggagacgatc atcaagatgt tctacgagcc gggcttcacg atcgatgacg ggatcgagga
     5101 gctggatcgg accacgtcgg acctcttccg cgaaggagtt gaacggggac tcgggggcga
     5161 gaccggggaa ggttgagcct ctccgtcgag agcgtcgcca aacgcttcgg tgaggtcgtc
     5221 gccctaacag gggtcgacct ctcggcggcc gacggcgagc tgttggtcat cgtcggcccg
     5281 tccggatcgg gaaagacgac cctcctgcgg tgcatcgcgg ggctcgagga gatcgactcg
     5341 ggtcgtgtat cggttgctgg tcgcgacgta accggggccg acccgggcga ccgggatgtt
     5401 gcgatggtct tccaggagta cgcgctctat ccgcacctta ccgtgcgagc caacatcgcg
     5461 ttcggcctca aggcccgcaa ggtagacgag agctccatcg agcgcagttg cgccgaagtc
     5521 gtcgagctcc tcggcttgca aaacacgacc gacaggcggc cggccgagtt gtctgggggc
     5581 gagcgtcagc gggtggctct ggcgcgagcg atcgtccgtg aaccggctct attcctgatg
     5641 gacgagccgc ttgcgaacct ggacgccgaa cttcggaccc acacccgcgg cgagatcaaa
     5701 cgactccaga agcgcatcgg gacaacgatg ctgtacgtga cgcacgatca gatcgaggcc
     5761 atgaccatgg gggacagcgt cgcggttatg cgtgccggca cgatcgagca gatcgactcg
     5821 ccgctcaacc tctacaacca ccctgcgaac gcgttcgttg cgaggttcat cgggcatcca
     5881 ccgatgaacg tgttccccgc cgaggtgctc tcgggcgaaa agcgtggtgc ctatgccggt
     5941 atcaggcccg aacgccttcg tctgggcgac ggccccggct ctatcgcggg tgaggtcgag
     6001 ctcgtcgagc cgatcggtgg tgaggttctc gttcacctga gtgtggactc gacccggttg
     6061 atcgcacgga tccccggcga cgagcatccg ccgataggga cctccgtcaa cgtcacgttc
     6121 ggggcggacg atgtgttctg gttcgagggc cccgacggga agacgttggc gtgagaggcg
     6181 cgacgaagct ggcgcttccg tacttggccg ggctgttcct tcttgtggcc gttccggctc
     6241 tgcttgcctt cgcccttgca ttcaccgagt tcccggccgt agcggctcct cgtttcaacg
     6301 gtgtggacaa cttcatccgt ctgttccgcg acgccggtta cgcgaccgcg ttgtggaact
     6361 cgttgatcta cctcgcactc tccgtaccgc ttcgcgttgt ggctgcggtt gggatcgcgc
     6421 tgctcatgta ccggaggagc cgtggcgtcg gagccgcacg cgcggccgct tacctgccga
     6481 cggttgtacc cgacgttgca ttcgggttgc tgtggctgtg gttgctgaat ccgctctacg
     6541 gacctatccc cggtgcactt caggccctcg gtcttccgtc acccgactgg ttgatcgacc
     6601 cgtggagcgc acgtttcgcg gttgcgatca tgagcgcgtt ccagatcggg gaagggttcg
     6661 tcatcgctct tgccgtacgc aggcttatcc catcttcgct ctacgaggcc gcgctgctgg
     6721 acggcgccgg tccgtggttc acgctcaccc ggctgacgct tccgctgatg gccccggcca
     6781 tagcgttgct cgcgctccgt gacgtcgttc ttgcactgca agcgaacttc gttccggcct
     6841 tgatcgtcac ggatgggggg ccgcggctcg cgacgacttt cctgtcgatc tatgtgtacc
     6901 gccaggcgtt cgcatacttc cgcctcggct acgcctctac gatctcacta accatgttcg
     6961 tgttgaccgc aggcatcgtt tacgcgcagt atcggctcgc ccgtcgctgg cggctcttat
     7021 gagcgatggg ctcggccatc gccctacccg gagcggaggg gattccggcg aagccgggaa
     7081 accttctgcg gagggaaaca tgagcgatgg gctcggccat cgccctaccc ggagggaaac
     7141 atgagcgcgt tcacggcgcg ccagaaggcg acgctggaag cactcgcccg cgttctactg
     7201 cccgaaggtc acggcctccc gggtggggat tccgccggcg ccgccgcggc tctcgatgct
     7261 cgcgccgaga cctgggacgg tcgcgtcctg cgcggcgtga aactgctgct cgacgcgtgg
     7321 gagctgtcgc cgctcgcgtc ccgccatcgg gcacgcttct cgatgcttgg agaggcggat
     7381 cgggtcgcgt ggaccatgca ttgctaccgc tccaaggctg catggcgccg gctgcaagtg
     7441 actgccctta aacaactgct gttcctggag tgggcctgct cgccggtcgt cgaggatgct
     7501 ctcggctacg actaccgatg tcggaaggaa ggcgtaccgc acggcgcggc ccgagcgctt
     7561 gtgggcgatc cggaccctgg gatcaccgaa ccggaacctg ccgactacca ccgcaccgga
     7621 ccggcggttg cgagcggggc catccccatc gcgttgatgt ccaagcgcga cttcgaaggg
     7681 ccccagtccc gggatctcac cactcaggca tggccggatc tgttcgacgg ccagagagag
     7741 aacgtggacg tggtggtcgt cgggtcgggg gccgggggag cggtcgcggc gacctacctc
     7801 gccgaagccg gtttgaacgt gctcgtactc gaagagggtc agcaggtcac ggccgatgac
     7861 ttcgccggtc cgatgttcga ccggctgcag aggttctgcc gcgggaacgg gacgacccag
     7921 gtctggggca gccctccgat cccgttgccg ctcggcaaag tcgtcggcgg cacgacggtc
     7981 gtcaattccg gcacctgctt ccgcggcccc cagcgcgtcc tcgaacgatg ggcgagcgag
     8041 ttcggcgttg aaggggcgcg gctcgacgag atgatcgagc actacgagcc tctcgaggag
     8101 tttctgaacg tgcggcccgt cccctgggac ctcttggggc cgaacggaat ggccgcgcat
     8161 cgtggtgccg tggcgctcgg ttactcgggg ggcccgttgc ttcgcaacat caccgactgc
     8221 catggttgtg gccagtgcgc cttcggctgt ccaacgaacg ccaagcaggc gatgcacgtc
     8281 tccttcctgc ctcgcgccca gatggcagga gcgcgcatct tctcccgttg tcgcgtcgac
     8341 acgatcgtga ccgagcgaga cggcgcggcc ggggtcgagg cgacgcttct ggggcccgac
     8401 ggtcgcagcc ggggccgtct gcgcgtatcg gcgacgcacg tggtggtggc cgcgggtgcc
     8461 gtctacacac cgagcttgct caagaagagc ggcgttccgg accccagtgg tcagaccgga
     8521 cgcaacctgc gtatccatcc ggctaccggc gtcggtgggg cgttcgacga ggacgtgaag
     8581 aactggaaag gcacactgca gagctattac atcgacgagt tcttcgacag tcacgagctg
     8641 atgttcgaag caaccaccac cgttccgagc atcggggctg gcgccctacc cgggatcggc
     8701 gagcaggcca tgcaggagct cggccggttc cgcaatctcg catctctcgg cttctatgtc
     8761 tccgacacat ctcgaggccg ggtgcttacg cttcgcggtg gcgaggcatt tcccgtgtac
     8821 aggatgaacg cattggatgc tcgtcggatg cagatcggat tgtcgatcgc gtgtgaggtg
     8881 atgctcgaag ccggcgccaa gaaggtctac cccgggatcc cgggcatcga cgcgatcgat
     8941 gcacgcgccg atctcgagca actgcgcgag cggcgcgtcc ggcccgagca cttgaaactg
     9001 acggcgttcc atcccatggg aaccgcacgt atgggaggcg acccgtcctc cagcgtcgtc
     9061 gatccttacg gacaccatca tgcggtcgag cgtctctggg tcgtcgactc gagcacgttc
     9121 ccgtcctgcg tagcggtcaa tccccagatg acgatcatgg ctttcgctaa gagaacggcg
     9181 gaatacctgg ctaggaaatt ctgatccgag gaggcagacg atggagctga gtggcagcaa
     9241 cgcgatcctg acgggcgcat cgcgagggct cggcgtgtat atcgccgacg ctctggccct
     9301 cgcgggtgtc aacctggcac tcgccgcgcg cgatcaaacc gggctcgatg cgaccgcgga
     9361 ccgcgtgcgg accaagggcg tgaaggtggc gacgatccca accgatgtgg gggatgagga
     9421 ttcgcttcgg aacctggccg atcaagccga ggagcagttg ggcccgatcg acatcctgat
     9481 caacaatgcc ggggtcgagc actacatcga cttccacacc gccgacacga agctgatcga
     9541 gaagatcatg cgcgtaaaca tcctggggcc ccaggtcctg actcgcttcg tgcttccggg
     9601 gatggtcgag cggaagcgcg ggcacatcgt gaacatcgcg tccgttgccg gcaagacgg
//
LOCUS       DATMED010000167         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5492766, whole genome shotgun sequence.
ACCESSION   DATMED010000167 DATMED010000000
VERSION     DATMED010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5492766"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VNP73_04245"
     CDS             complement(<1..778)
                     /locus_tag="VNP73_04245"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HWL65166.1"
                     /translation="MGKSYFVTTYGCQMNEHDSERLAGLLEAEGYSKAAAPEEADVVL
                     FNTCCIREKADQRLYGNLGHLKPVKDARPGMKIVVGGCLAQKDRSLIQQKAPYVDVVL
                     GTHNLGSLPRLLRESEEGPAFEILEQTEVFPSALPARRSSPWHAWVSISIGCNNSCTF
                     CIVPAVRGEEISRRTGDIVAEVESLVADGVVEVTLLGQNVNSYGRDLDGTPLFSKLLY
                     ALDGIDGLERVRFTSPHPKDFRADTVAAMAECRSVCEHIHL"
     gene            868..1320
                     /locus_tag="VNP73_04250"
     CDS             868..1320
                     /locus_tag="VNP73_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWL65167.1"
                     /translation="MKKLARSLDSAGYLGTLALRIGVGLTMAWHGWQKIDGGVTNFAG
                     FVESLGIPAPSLMAYLVTGLELLGGIALIAGALTRPIAALIAIEMLLTGFWVKGSKLD
                     VSFIAPEGAGIELDFVLMAGALALVFWGPGRLSVDHAAGLDRPAASPA"
     gene            1424..1594
                     /locus_tag="VNP73_04255"
     CDS             1424..1594
                     /locus_tag="VNP73_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65168.1"
                     /translation="MDKKLELNTETLRELTDVELTDVGGGREEITKNVTQSPVCPSGA
                     TWLQQCVTGSWC"
     gene            1644..2921
                     /locus_tag="VNP73_04260"
     CDS             1644..2921
                     /locus_tag="VNP73_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HWL65169.1"
                     /translation="MFHIDIETSTVRDDTSSRPWRPVVHGAVADAALAAALDLGRVIA
                     TLERDPDPTLAKGTAGQAVALAYLARQWPGEGFEKTSHELLSAAGNSVASERMTIGLM
                     QGFTGIAWAVNHLSWGGEDATEQIDDALIGLLEKPGWRGEYDLVSGLAGIGTYGLERA
                     GTEAGLRLVELVVDRLIERSETIDGRTRWFTPPEFILAAKLGRAPKGFYNLGMAHGLP
                     AVVAFLADACEAGIAVDKALPVLRGAVEYLLSERLDGADSTWPYFVGEEVEIMPARLA
                     WCYGDLGVLSALRRAVAVTGDETWGAALHTGLDRVLKRSPETSGVTGVSLCHGTAGLA
                     LMFARLSFHLDSEELAAEAARWIEATLRLQDPSKPLGGFSVWDPDSRDEEGDVQGPGL
                     LAGIAGVALALLACASRHAPSWDRALMLSGPAQ"
     gene            2918..>3732
                     /locus_tag="VNP73_04265"
     CDS             2918..>3732
                     /locus_tag="VNP73_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HWL65170.1"
                     /translation="MSPRKQLDFGPSGFFALRTPLLSFEEILRRPDLADVIRRPEVRE
                     AIFLASPKLESQIDGWLGDSPENRDVKVRRALTRYFLRMSARPTPFGTLAGTSVGRVG
                     EATDIRLSEPADHRRRTRLDMHYLSALAEALQDPSLIRSLRLHPNTSLYEAAGRLRYA
                     EARYEGGARRYRLVAVEPTEGVVIVLGRARDGASFDDLVDALVDDEVTEADASAFVFR
                     LIESQLLVSDLEPSVTGVDALDSILETLGSISEASGPAIVLKEARAALESIDAA"
BASE COUNT          649 a         1174 c         1217 g          692 t
ORIGIN      
        1 gcaggtggat gtgctcgcag acgctcctgc actcggccat ggccgcgacc gtgtcggcgc
       61 ggaagtcctt ggggtgggga ctcgtgaaac gaacgcgttc caagccgtcg atcccgtcga
      121 gtgcatagag aagtttcgag aacaatgggg ttccgtcgag gtctcggccg taggagttca
      181 cgttctgccc gagcagtgtg acttcgacga ccccgtcggc taccagactc tccacttcgg
      241 ccacgatgtc gccggtcctg cgggagatct cctcgccgcg cacggccggg acgatgcaga
      301 acgtgcacga gttgttgcag ccgatcgaga tcgacaccca tgcgtgccac ggcgagctcc
      361 gacgcgcggg aagcgccgac ggaaagacct ccgtctgttc gaggatctcg aacgcaggac
      421 cctcttcgga ttctcgcagc aggcgaggca ggctccccag gttgtgcgtc ccaagaacaa
      481 catcaacgta cggggccttc tgctggatca ggctgcggtc cttctgcgcc agacaacccc
      541 cgacgacgat cttcatcccg ggtcgggcat ccttcaccgg cttgaggtgc ccgaggttcc
      601 cgtacaaacg ctggtcggcc ttctcccgga tacaacaggt gttgaagaga acgacgtcgg
      661 cttcctcggg agccgctgcc ttcgagtacc cctcggcctc gagcaacccg gccaaacgct
      721 ccgagtcgtg ctcgttcatc tggcagccat aggtggtgac gaaatagctc tttcccatcg
      781 ccacggagcc tagcccggta ccgacgaatc tcccgtcggg cccgctgcgt accatctgtg
      841 gaagtccaca tcgactgggg ggtggtcatg aagaagctcg cgcgcagcct cgattcggcc
      901 ggatacctgg gaacccttgc gctcaggatc ggggtcggac tgacgatggc ctggcatggc
      961 tggcagaaga tcgacggcgg agtgacaaac ttcgccggat tcgtcgaatc gcttgggatc
     1021 ccggccccct cgttgatggc ctacctcgtg accgggctcg agctgctggg aggcatcgcc
     1081 ctgatcgccg gagcgctcac ccgtccgatc gccgcattga tagcgatcga gatgctcctg
     1141 accggcttct gggtgaaggg atcgaagctc gacgtctctt tcatcgcgcc ggagggtgcc
     1201 gggatcgagc tcgatttcgt gctcatggcg ggagccctcg ccctcgtgtt ctgggggccg
     1261 ggaaggttgt ccgttgatca cgcggccggc ctcgaccgcc cggcggcatc ccccgcctga
     1321 gagattcctc gccggcgcgg gaggactccg gaccgtcgag cggaacttgt tcaagcaaaa
     1381 gcgggcgtag ccctagacac ttcgcgcctc gaagggggtg gatatggaca agaagctcga
     1441 actcaacacc gaaactctgc gggagctgac cgacgtcgag ttgacggacg tcggaggcgg
     1501 cagggaagag atcaccaaga acgtgacgca gtcaccagtg tgtccctcgg gagccacctg
     1561 gttgcagcag tgcgtaacag gtagctggtg ctaagaagga tcgactcaca ggttaggact
     1621 cacgagcggc cccagggccg ctcgtgtttc atatagacat cgaaacatcg acagttcgcg
     1681 acgacacttc ctcacggccg tggcggccgg tcgttcacgg cgccgtggcc gatgcggcgc
     1741 ttgctgctgc cctcgacctc ggtcgcgtca tcgcgacgct cgaacgggat cccgatccaa
     1801 ccctggcgaa gggcaccgca gggcaggcgg tcgcgctcgc gtacctggcg cgccagtggc
     1861 ccggcgaggg cttcgagaag acctctcacg aactgctttc tgcagccgga aacagcgtcg
     1921 cctcggagag gatgaccatt ggcctgatgc agggcttcac cggcatcgca tgggccgtaa
     1981 accacctgtc gtggggaggc gaggacgcga ccgaacagat cgacgacgcg ttgatagggc
     2041 ttctcgagaa acccgggtgg aggggcgagt acgacctcgt gagcggcctc gcaggtatcg
     2101 ggacctacgg tctcgagcgc gccggcactg aggccgggct ccgtctggtc gagctcgtcg
     2161 tggatcgctt gatcgaacgc tcagaaacga tcgatgggcg cacgcgctgg ttcacgccgc
     2221 ccgagttcat cctggctgcg aagctcggtc gcgcacccaa gggcttctac aacctcggga
     2281 tggcgcacgg acttccggcc gtggtcgcat tcctcgcgga tgcttgcgag gcaggcatcg
     2341 cggtggataa agccctaccg gttcttcgcg gcgccgttga atatctcttg agcgaacgtc
     2401 tcgatggggc cgattcgacc tggccctatt tcgtcggaga agaggtcgag ataatgccgg
     2461 cgcgtctcgc ctggtgttac ggagatctcg gcgttctgag tgccttgcga cgggccgtcg
     2521 ccgttaccgg cgatgagacc tggggggcgg cgttacacac tggtctcgat cgagtcctca
     2581 agcggtctcc cgagacgagc ggtgttaccg gtgtgagtct gtgtcacgga accgccgggc
     2641 tcgcgttgat gttcgcgcgg ctctcctttc atctcgatag tgaagagctc gcggccgaag
     2701 ccgctcgctg gatcgaagcc acccttcggt tacaagatcc atccaaaccg ttggggggct
     2761 tctccgtgtg ggacccggat tcgagggacg aggagggaga cgttcaggga ccgggtcttc
     2821 tggcgggcat agccggtgtt gcgctggcct tgctcgcgtg cgcgtcccgg cacgcaccca
     2881 gttgggatcg cgcgctgatg ctgtcgggcc ccgctcaatg agccctcgca agcagctcga
     2941 tttcggcccc agcggattct tcgcactgcg cactcccctc ctcagcttcg aagagatcct
     3001 ccggcggccg gacctcgccg atgtgatccg aaggccggag gtccgcgagg ccatcttcct
     3061 ggcatcgccg aagctcgaat cccagatcga tggttggttg ggggactcgc ccgagaaccg
     3121 ggatgtgaag gttcggcgcg ccctgacgcg ttacttcttg cgcatgagcg cacgccccac
     3181 accgttcggc acactggccg gaaccagcgt cggtcgggtg ggcgaggcca ccgatatccg
     3241 tttgtcggaa cctgccgatc accgacgaag aacgcgactc gacatgcact acctgtctgc
     3301 gctcgcggag gctcttcagg atccctcgct tatccggagt ctccggctgc atccgaacac
     3361 gagcctctat gaagccgccg gtcgcctgcg ctacgcggaa gcaaggtacg aaggtggagc
     3421 tcgccgttac cggctcgtcg cggttgaacc cacggaaggg gtggtgatcg tgctgggtcg
     3481 cgcccgagac ggcgcatcct tcgacgatct ggtggacgcg ctcgtagacg acgaagtcac
     3541 cgaggccgac gcgagtgcat tcgtgttccg actcatcgag tcccaactgc tcgtctccga
     3601 tctggaacca tccgtcacag gcgtggacgc gctcgactcg atattggaaa cgttggggtc
     3661 gatctctgaa gcctccggtc ccgcgatcgt tctcaaggag gcgcgtgctg cactcgagtc
     3721 gatcgatgca gc
//
LOCUS       DATMED010000168         5559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4735933, whole genome shotgun sequence.
ACCESSION   DATMED010000168 DATMED010000000
VERSION     DATMED010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4735933"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VNP73_04270"
     CDS             <1..392
                     /locus_tag="VNP73_04270"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HWL65171.1"
                     /translation="RFVRVEVPRTSGIRLQGRPVLRLEASASQVSTHFVAVLLDVSKT
                     GQTQVISRAFLNGRYKDGLTKGKDLEPGKATTFDLEFIDKDWIVAPDHHLELLIASSS
                     NSWVFPDEQRSNNTLFLKRSRLLLPVR"
     gene            531..1115
                     /gene="pyrR"
                     /locus_tag="VNP73_04275"
     CDS             531..1115
                     /gene="pyrR"
                     /locus_tag="VNP73_04275"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWL65172.1"
                     /translation="MAGALRAQEFALKAQVMDASDVRRALTRIAHEIVERNKGTEDLV
                     LVGIRTRGAPLAERLAAAIASFEGGDLPSGALDVTMYRDDVALRGPRGIESTTIPVDI
                     DGRIVVLVDDVLYTGRTIRAAFDAVLDLGRPRATCLAVLVDRGHREFPIRADFVGKNL
                     PTAADEVVKVHLDEIDGEDAVLIGKWRPVGDEKR"
     gene            1112..2035
                     /locus_tag="VNP73_04280"
     CDS             1112..2035
                     /locus_tag="VNP73_04280"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWL65173.1"
                     /translation="MIKHLLSMDQLDRDQIDTILETAQSLRQVTDRRMKKLPTLRGRT
                     VCNLFYEASTRTRISFEVAAKRLSADVINFTADAKSSVAKGESLKDTAWTLEAMGVDV
                     IVLRHWASGAAQQLSEWVAASVVNAGDGQHEHPTQALLDLYSMREHFKDLEGLKVAIV
                     GDILHSRVARSNVKGLVTMGAEVVLVGPPTLVPKLPGADVQPDFDSVIGEVDVCYLLR
                     VQKERQADQFFPTNREYAAMWGLNARRAARMKPHALIMHPGPMNRGVEIAADVSESPK
                     SIIVEQVANGVAVRMSLLYLMLGSENGEGMQ"
     gene            2032..3348
                     /locus_tag="VNP73_04285"
     CDS             2032..3348
                     /locus_tag="VNP73_04285"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWL65174.1"
                     /translation="MSPSYLLTGGRVVDPASGRDEIADVAISNGLIEDAGSVAHPDAE
                     TIDVSGHIVCPGFVDLHTHLREPGREDEETIASGSAAAAAGGYTAICAMPNTDPVADT
                     ASIVEKVWALGRETGLVEVVPAGALTKGLLGERMSDIGEMHRCSAGVRLFTDDGKGVQ
                     DALMVRRLMEYIAGFGAVYAEHCEVESLARGGHMHEGERSLALGLAGIPAAAEELMVV
                     RDIALARLTGCRLHILHVSTTGAIEAIREAKRDGLVVTTEATPHHFTLTDSALEGYDP
                     RAKVNPPLREEADRLAVIEGLVDGTIDAIATDHAPHAREEKDQELTQAPPGLIGLETA
                     FALAMTELVAPGHLSLSTLIERLSTAPARILGLEAHGGPLEPGRPANLTVVDPEAEWV
                     VDPDAFRSLSTNTPFAGRRVTGRVLHTFYYGRATVLNGEVRGTVSV"
     gene            3345..4577
                     /gene="carA"
                     /locus_tag="VNP73_04290"
     CDS             3345..4577
                     /gene="carA"
                     /locus_tag="VNP73_04290"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWL65175.1"
                     /translation="MSGIFKDNGPPALLALEDGSVWRGSAFGARATITGEVCFNTGMT
                     GYQEVLTDPSYHGQIVTMTSVQIGNTGVNDFDDQSIKPWVAGFVVRDVSPHPSSWRAT
                     GSLQDYLDRHGVPGISGIDTRRLTRHLRTHGALRGALSTEVVDGDELVDRARRSPRMV
                     GLDLAAAVSTTESYEWGPAQLAERALIGYAAGAEVAPSSAALLAEHVGRRFKVAAFDL
                     GIKRNILDLLVASGCDVTVVPASTSAADIVSGGYDGVFLSNGPGDPEPVTYAIHTVAE
                     LLGRVPIFGICLGHQILGLAIGGKTYKLLFGHRGSNHPVKRFDDPRVEITCQNHGFAV
                     DPDSIDATPARLTHVNLNDKTVEGLEVPGSAFSVQYHPEAGPGPHDSRYLFERFRTLM
                     TEFEPAEIRLESTVPTTS"
     gene            4627..5139
                     /locus_tag="VNP73_04295"
     CDS             4627..5139
                     /locus_tag="VNP73_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL65176.1"
                     /translation="MEEGPPEEEARAREAQGRGARGGTQIDDDYTILGIEHVHITAPS
                     ELLEETIEWYRSCLRLEPVEKPKGTRPDGAWFAAGPQELHISVDEHNPPKVSHFALVF
                     DAYEPVIESLREQGCHIEQAATIPGRHRCYTRDPAGNRVEIVHYDEVEVVALETARGE
                     LRAKVMHEER"
     gene            5144..>5559
                     /gene="carB"
                     /locus_tag="VNP73_04300"
     CDS             5144..>5559
                     /gene="carB"
                     /locus_tag="VNP73_04300"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593993.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HWL65177.1"
                     /translation="MPKRDDLKKILVIGSGPIVIGQACEFDYSGVQACKVLRAEGYEV
                     VLVNSNPATIMTDPEFADATYVEPITAEYVAKVIAKERPDACLPTMGGQTGLNVAMEL
                     AASGVLDAHSVELIGADTEAIRRAEDRRLFKETMAAA"
BASE COUNT         1041 a         1771 c         1809 g          938 t
ORIGIN      
        1 accgtttcgt gcgagtagag gtgccgcgga cctcgggcat ccgattgcag gggcggccgg
       61 tactgaggct cgaagcgtct gcctcccagg tcagcaccca cttcgtcgcc gtcctcctcg
      121 atgtatcgaa aaccgggcag acgcaggtga tctcccgagc gtttctgaac ggccgttaca
      181 aggacggcct caccaagggc aaagacctcg agccgggcaa ggccaccacc ttcgacctcg
      241 agttcatcga caaggactgg atcgtggcgc cggaccatca cctcgagttg ctcatcgcct
      301 cgtccagcaa cagctgggtt ttccccgacg agcagcgctc caacaacacc cttttcttga
      361 agagatcccg cctactgctt cccgtccgct aggcgattcg ctactagcgg gtcgccaacg
      421 cgcgcgataa ggtgtgtcga aacgcccttt aaatcggccc tgagaggccg gaaaggagca
      481 ggcttgggta cgacgcgtac gccttcttcg ccggaagagg gcgttttttg atggcgggag
      541 ccttgagagc ccaagaattc gcgctcaaag ctcaggtgat ggacgcctcc gacgtccgcc
      601 gggccctaac tcgcatcgca cacgagatcg tcgaacggaa caaggggacc gaggatctgg
      661 tcctggtggg gatccggacc cgaggcgcgc cgctggccga gcgactggcc gccgcgatcg
      721 cgtccttcga ggggggagac cttccctccg gcgcgctgga cgtgacgatg tacagggacg
      781 atgtggcgct gcgcggcccc cgcggcatcg aatcgacgac gatcccggtc gacatcgacg
      841 ggcgcatcgt tgttctggtc gacgacgtgc tctacaccgg ccgcacgatc cgggccgcgt
      901 tcgacgcggt cctcgatctg ggtcggcctc gggccacctg tctggccgtt ctggtcgacc
      961 gcggccaccg cgagtttccg atccgagccg atttcgtcgg caagaacctc ccgacggcgg
     1021 cagacgaagt ggtgaaggtc catctcgacg agatcgatgg agaggacgcg gtgttgatcg
     1081 ggaagtggcg gccggtgggg gatgagaaac gatgatcaag cacctgctat cgatggatca
     1141 gcttgatcgg gatcagatcg acacgatcct ggagaccgca cagtcccttc ggcaggtcac
     1201 cgacagacgc atgaagaaac tcccgacgct tcggggccgc accgtctgca atcttttcta
     1261 cgaagcttcc acgcggacgc gcatctcttt cgaggtcgca gccaagcggc tctcggccga
     1321 cgtcatcaac ttcaccgcag acgcgaagtc ttcggtcgcc aaaggcgagt cgctgaaaga
     1381 caccgcgtgg acactcgaag cgatgggcgt cgatgtgatc gtgcttcgtc actgggcatc
     1441 cggcgccgcc cagcagttgt cagagtgggt ggccgccagc gtcgtgaacg cgggcgacgg
     1501 ccagcacgag cacccgacgc aggcgctcct cgatctgtac tcgatgcgcg agcacttcaa
     1561 agacctcgaa ggtctcaagg tggcgatcgt cggcgacatc cttcactcac gcgtggcccg
     1621 gtccaacgtc aaagggttgg tgacgatggg tgccgaagtt gtactggtgg gccctccgac
     1681 gttggtgccc aagcttccgg gggccgacgt gcaacccgac ttcgattcgg ttatcgggga
     1741 agtcgatgtc tgttacctgc tgcgcgtcca gaaggaaagg caagcggatc agttcttccc
     1801 gacgaaccgt gaatacgcag cgatgtgggg gctcaacgcc cggcgcgcgg cgcgcatgaa
     1861 accacacgct ttgatcatgc atcccggccc gatgaatcgc ggggtcgaga tcgcggcgga
     1921 cgtgtccgaa tcaccgaaat ccatcatcgt ggaacaggtc gccaacggag tagccgtccg
     1981 gatgagcctc ctctatctga tgctgggatc cgagaacggg gaggggatgc aatgagcccc
     2041 tcctacctcc tcacaggcgg acgcgtggtc gaccctgcct ccggtcgcga cgagatcgcc
     2101 gacgttgcga tatcgaacgg tctcatcgag gacgcggggt cggtagccca ccccgatgcc
     2161 gagacgatcg acgtctccgg ccacatcgtt tgtccggggt tcgtcgatct gcacacgcac
     2221 ctgcgtgagc ccggccgaga ggacgaggaa acgatcgctt ccggctcggc agccgcggct
     2281 gccgggggtt acaccgccat ctgtgcgatg ccgaacaccg atccggtcgc cgacacggca
     2341 agcatcgtcg agaaggtctg ggccctcgga cgggagaccg ggctggtcga ggtcgttcct
     2401 gcgggtgcac tgacgaaagg tctgctcggc gagcggatgt cggacatcgg cgagatgcac
     2461 cggtgcagcg ctggggtcag gttgttcacc gacgacggga agggcgtcca ggacgccttg
     2521 atggtgcgcc gcttgatgga atacatcgcc ggattcggcg ccgtctacgc cgagcattgc
     2581 gaggtcgagt ccctggctcg cggcggtcat atgcacgaag gcgagcgttc tctggctttg
     2641 ggtctggccg gtataccggc ggctgccgag gaactgatgg tcgtcaggga catcgctctg
     2701 gcgcgcctga ccgggtgccg ccttcacatc ctccacgtat cgacgacggg ggcgatcgag
     2761 gcgatcaggg aggcgaagcg cgacggcctc gtggtaacca cggaggccac tccacaccac
     2821 ttcacgctga ccgattccgc cctcgagggc tacgacccgc gtgcgaaggt caaccctccg
     2881 ctgagggaag aggccgaccg cctcgccgtg atcgaagggt tggtcgacgg cacgatcgac
     2941 gcgatcgcca ccgaccacgc tcctcatgcc cgggaggaga aggatcaaga gctcacgcaa
     3001 gcccccccgg gtcttatcgg gctcgagacc gcattcgccc tggccatgac cgagctcgtg
     3061 gctccgggtc acctgtcgct gtcgacattg atcgagcgcc tttcgaccgc tccggcgagg
     3121 atcctcgggc tggaggctca cggaggacca ctcgagccgg ggcgtcccgc caatctcacg
     3181 gtggtcgacc cggaggccga gtgggtcgtc gatccggacg ctttccggtc gctgtcgacc
     3241 aacaccccgt tcgccggccg acgggtgact gggcgggtcc tccacacctt ctattacggt
     3301 cgagccacgg ttctcaacgg agaggtgcga gggacggtga gcgtatgagc gggatcttca
     3361 aggacaacgg ccctcctgct ctcctcgcgt tggaggacgg atcggtgtgg cgaggtagcg
     3421 cgttcggcgc tcgcgccact atcaccggcg aggtctgctt caacacgggc atgaccggat
     3481 accaagaggt gctgacggat ccctcgtatc acgggcagat cgtcacgatg acctccgtgc
     3541 agatcgggaa caccggcgtc aacgacttcg acgaccagtc gatcaagccg tgggtcgccg
     3601 gcttcgtcgt gcgcgacgtc tctccgcacc cttcgtcgtg gcgagcgacc ggatcgttgc
     3661 aggactatct cgaccggcac ggggttcccg gcatctccgg gatcgacacc cgccggctga
     3721 cgcgtcatct ccggacccat ggagcgctcc gaggtgcact atctaccgag gtcgtcgatg
     3781 gggacgagct ggtcgaccgc gcccgccggt caccgagaat ggtcgggctc gacctggcgg
     3841 ctgccgtgtc gacgacggag tcctatgagt ggggcccggc tcagctcgcg gagcgagccc
     3901 tgatcggcta tgccgcgggg gccgaggtgg ctccttcctc agccgctctt ctcgcagaac
     3961 acgtcgggcg ccgattcaag gtcgcggcgt tcgacctagg catcaagcgc aacatcctcg
     4021 acctcctcgt cgcgagcggt tgcgacgtga cggtcgtccc cgcctcgacc tcggccgccg
     4081 acatcgtgag cggagggtat gacggggtct tcctatcgaa cggcccgggg gatcctgaac
     4141 cggtcactta tgcgatccac accgtcgccg agctgctcgg gcgggtccct atcttcggca
     4201 tctgcttggg gcatcagatc cttgggctcg ccatcggcgg caagacctac aagctcctct
     4261 tcggacaccg cggctccaac catcccgtga agagattcga cgacccgcgc gtcgagatca
     4321 cttgtcagaa ccacggcttc gcggtcgatc cggattcgat agacgcgacg ccggctcgcc
     4381 tcacgcacgt gaacctcaac gacaagaccg tcgaaggcct ggaggtcccg gggagtgcgt
     4441 tcagcgttca gtaccacccg gaggccggtc ccggccccca cgactcccgc tatctcttcg
     4501 agcgtttccg caccttgatg accgagttcg agccggcgga gatccggctg gaatcgaccg
     4561 ttccgacaac gagctagggc gaaaggagaa cctgtatatg ggccgaggag accgcaaacg
     4621 gactcgatgg aagaaggacc gccagaagaa gaagcgcgag cgagagaagc gcaaggccga
     4681 ggcgcgcggg gaggcacgca gatagacgac gactacacga tcctcgggat cgaacacgtt
     4741 cacatcacgg caccatccga gttgctcgag gaaacgatcg agtggtaccg gagctgtctc
     4801 cgtctggaac cggtggagaa gcccaaggga acgcgtcccg acggagcctg gttcgcggcg
     4861 ggtcctcagg agctccacat atcggtggac gaacacaacc cgccgaaggt gtcgcatttc
     4921 gccctcgtct tcgacgccta tgagcccgtg atcgagtctc tgcgggagca ggggtgtcac
     4981 atagagcagg ccgcgacgat ccccggccgc catcgctgct acacgcgcga tccggcgggg
     5041 aaccgtgtcg agatcgtgca ctacgacgag gtcgaggtag tcgccctcga aacggcgcgc
     5101 ggcgagttac gcgccaaggt gatgcatgaa gagagataac tagatgccca aacgagacga
     5161 cctgaagaag atcctggtga tcggttccgg accgatcgtg atcgggcagg catgcgagtt
     5221 cgactactcc ggggtccagg cctgcaaagt gctgcgcgcc gagggctatg aggtcgttct
     5281 ggtgaactcc aaccctgcaa cgatcatgac cgaccccgag ttcgcagacg cgacctacgt
     5341 cgagccgatc acggccgagt acgtcgccaa agtgatcgcc aaggaacgac ccgacgcctg
     5401 cctcccgacc atgggcggcc agaccggttt gaacgtcgcg atggaactcg ccgcgtcggg
     5461 ggttctcgac gcacactccg tcgaactgat cggtgccgac acggaagcga tccgaagagc
     5521 cgaagaccgt cgcttgttca aggaaacgat ggccgcagc
//
LOCUS       DATMED010000169         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1666910, whole genome shotgun sequence.
ACCESSION   DATMED010000169 DATMED010000000
VERSION     DATMED010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666910"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VNP73_04305"
     CDS             <1..433
                     /locus_tag="VNP73_04305"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWL65178.1"
                     /translation="TDFDFSLLDHPNSVEGGKRPRSSMSPTIVLDDGRPVLAVGSPGG
                     SMIITTVLQILANHLDFGMSLPDAIAAPRASQRNTAAVFAEPAFRSAWEAALAARNHS
                     FATSAEIGAATGIAFQPDGSVVAAAEPVRRGGGSAMVENPD"
     gene            complement(1199..1366)
                     /locus_tag="VNP73_04310"
     CDS             complement(1199..1366)
                     /locus_tag="VNP73_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65179.1"
                     /translation="MLIAYRGAAEMVGWDTLYSIRSFFHYSTRQLEALMTDANLRLLQ
                     AENKNKLFRRD"
     gene            1409..1798
                     /locus_tag="VNP73_04315"
     CDS             1409..1798
                     /locus_tag="VNP73_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65180.1"
                     /translation="MPANSGTASVRFSHEPKGTSLRILADSEVVSGMNYGAVKLDDSL
                     KSVRNVIHNEIRQRECVARAGSAFMDTYLGRIGTSLPTLSFATFASIELNVQHTLPES
                     EGALRVIGWKLDEGERRAIHTPTISGA"
     gene            1901..2158
                     /locus_tag="VNP73_04320"
     CDS             1901..2158
                     /locus_tag="VNP73_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65181.1"
                     /translation="MTEGLRPAKGEHHHKTMRKESFPDKVFETIDENAGGVDEVMEPP
                     ELLGPMLRGQICDDVLGCDIGCGVEVRRVLWRSSQSARRKG"
     gene            2155..2598
                     /locus_tag="VNP73_04325"
     CDS             2155..2598
                     /locus_tag="VNP73_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HWL65182.1"
                     /translation="MTDLEAFAKLVPLDHGLVVVSTSRANGTIQSSVVNAGVLGHPIS
                     GKQVVAFVAAGSSQRLVNLRARPRATVVIRAGWQWAGVEGPVELAGPDDPLDGIDGER
                     LRLLLREIFTAAGGTHDDFDEYDRVMGEERRVAVLVEPERVYGTG"
     gene            complement(2737..3078)
                     /locus_tag="VNP73_04330"
     CDS             complement(2737..3078)
                     /locus_tag="VNP73_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HWL65183.1"
                     /translation="MPVINDYRFGHIVIDNREHSRDVIVLPNRVVPNWWRKDGHSLVA
                     EDLAEVIEELPSNLIVGCGAHGRLVPDPHAIEELERRGINIEILPTEDAVNRYRQLDP
                     ATAAAALHLTC"
     gene            complement(3088..>3667)
                     /locus_tag="VNP73_04335"
     CDS             complement(3088..>3667)
                     /locus_tag="VNP73_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HWL65184.1"
                     /translation="SVAFDHRPGNDDVISVYPMFESFDIRPILRLRPQPLRDTRFVVD
                     SNLGGLARDLRMLGFDTLFQNDFADDEIARISASDQRLLLTRDVGVLKRKEITHGYYV
                     RASEPIEQSSEVVRRFDLATSLRPFTRCLECNESLEDATPQEVRQLIPNGAAREHTEF
                     TRCPNCGRVYWAGSHYIRMKKKIDEILERVRS"
BASE COUNT          715 a         1103 c         1158 g          691 t
ORIGIN      
        1 caccgacttc gacttctccc ttctcgacca ccccaacagc gtcgagggcg ggaagcgccc
       61 gcgtagctcg atgagcccga cgatcgtgct cgatgacggc cgcccggtgc ttgcggtggg
      121 atcgccgggc gggtccatga tcatcaccac cgttcttcag atcctggcga accacctcga
      181 cttcgggatg agcctcccgg acgcgatcgc cgcaccgcgg gcttcacagc gcaacaccgc
      241 agctgtcttc gccgagcccg ccttccggag cgcgtgggag gcggcgttgg ccgcgcgcaa
      301 ccacagcttc gcgaccagcg ccgagatcgg ggcggccacg ggcatagcct tccaaccgga
      361 tggatccgtg gttgcagccg cggaaccggt gcggcgcggc ggcgggagtg ccatggtcga
      421 gaatccggac tagagggcgg tgtacgcgac caactcgacg cgcttcgcgg tcgcctctgc
      481 cgatccctga gccgtgcgct gcgctcgcac ctcctccgtc agtcgcgacg agcccgggcg
      541 cggcgcttgc cctcatgcat cgcccgcacg cgggtgatgg ggatgactcg tcctcctcga
      601 tcaagtcggc ccccaatgac tgcggcggtt gcttcgcgcc gttccttgga tctcgaacga
      661 ccgagcctgg gctcggctac aggtggctgc ccgagccgca acgtagggag tagcgcgtaa
      721 gaacctccgg acataacggc ttcgacccga acccggacag aacgacagaa aaatggcact
      781 cacgaaggga accgttcacg aggaccctgt gaatcaccag aaacagcccc ccgcgccgcc
      841 gcttcaacta ctcgaacgtg atcttgacga tcgcggtctt cggcgcgctc gccggcgcgg
      901 gcggcgcctg ggccgcctcg cagttcagct ccgacgtcat caagggggga acgcggtgaa
      961 gaccaagcac accaagaagg ggcggtcaag acgccgaaga tcgccgcttc atacgtgacc
     1021 aacatcgaac gtggtgttct tgacacctat cgcattgagg gcatgcattc gatacgaaga
     1081 tgccgaacct ttcgcttcca gcacgcatcg ggctaccgcc tgcacacccg cgctgtcgct
     1141 agaaaggcga gtgagccaac ccatccatgg atggctagat gggtttgagt cttccgcatc
     1201 aatctctccg gaaaagcttg ttcttgttct ccgcttgcag gagtctgagg ttcgcatccg
     1261 tcatcaaggc ttccagctgt cgagtggaat agtgaaagaa cgatcggatc gagtaaaggg
     1321 tgtcccagcc caccatctcg gccgcgccac ggtaagcgat gagcaatgat tggccgcctg
     1381 gataccgacg tgtaatacat ttgcgggcgt gcccgccaat tctggcacgg cctcagtgcg
     1441 gtttagccat gagcccaaag ggacttccct cagaatcctt gcagatagcg aagtggtctc
     1501 cgggatgaac tacggtgccg tcaagctcga tgactcgttg aagagcgtcc gcaatgtcat
     1561 ccacaatgag atacggcaac gagaatgtgt cgcccgggcc ggttccgcgt ttatggacac
     1621 ctatctcggc cgcatcggaa cgagtctgcc aaccctgtcc ttcgccacct ttgcgagcat
     1681 cgaactcaat gttcagcaca ccttgccaga atctgagggc gcgctccggg tcatcggctg
     1741 gaaactcgac gagggagaga ggagagcgat ccatacgccc acaataagcg gtgcttgagg
     1801 aagatggtca gcctcggctg ttatgccatg aagcgaatcc aagggggaga gcaggggacg
     1861 cttccgcccg cgtcaagaag ttgtcacaac tgcggaggac ttgaccgaag gactacgacc
     1921 ggcaaagggc gagcaccacc acaagacgat gcgcaaggag tccttcccgg acaaggtctt
     1981 tgagacgatc gatgaaaatg ccggcggtgt cgatgaggtt atggaaccgc cggaactcct
     2041 cggcccgatg ctgcggggtc agatctgcga tgacgttctc ggatgcgaca ttggatgcgg
     2101 cgtagaggtt cgtagggtgt tgtggcgttc gagtcaatcg gctagaagga agggatgact
     2161 gacctcgagg cgttcgcaaa gctcgtgccg ctcgatcacg ggctcgtcgt tgtgtcgacg
     2221 agccgggcga acgggacgat ccaatcgtcg gtcgtgaacg ccggcgtcct cggccacccg
     2281 atctcgggca agcaggtcgt cgcgttcgtg gcggcggggt cgtcccagcg cctcgtcaac
     2341 ttgcgcgccc gtccccgcgc caccgtagtg atccgggccg gttggcagtg ggctggtgtg
     2401 gaggggccgg tcgagctcgc cgggcccgac gatccgctcg acgggatcga cggcgaacgc
     2461 ctgcgtctgc tgcttcgcga gatcttcacc gccgcgggcg ggacccacga tgacttcgac
     2521 gagtacgacc gggtcatggg cgaggagcgt cgggtcgccg tcctcgtcga gcccgagcgc
     2581 gtgtacggca ccggctgatc gtctgcactg cccacgtagc tcggacaggc gattcgtaaa
     2641 ggtcacagac gaacgggaca tcgctcgtgc ggtcctggaa ttcctcttca ttctgtcgtc
     2701 cttgtcgtca ggaccgattt ccggggtggt tatgggctag caggtgaggt gcagcgctgc
     2761 ggcagcggtt gccggatcca gctggcgata acggtttacg gcatcctcgg tcggaaggat
     2821 ctcgatgttg atcccgcggc gttcgagctc ttcgattgcg tgaggatccg gcactaaacg
     2881 accgtgggcc ccgcagccga cgatgagatt tgaggggagt tcctcgatga cctcggccaa
     2941 gtcctcggcg acgagcgagt gcccgtcttt gcgccaccag ttggggacaa cgcggttcgg
     3001 aaggacgatc acgtccctgg aatgctcccg gttgtcgatg acgatatgac cgaagcggta
     3061 gtcgttgatg acaggcatcc gcccactcta cgaccgaacg cgctcgagaa tctcgtcgat
     3121 tttcttcttc atccgaatgt agtgagagcc cgcccagtag acgcgtccac aattcggaca
     3181 gcgagtgaat tcggtgtgtt cgcgtgctgc cccattcggt attagttgtc tcacttcttg
     3241 gggggtagcg tcctctaacg attcgttgca ttccagacag cgcgtgaacg ggcgcaggct
     3301 ggtggcgaga tcgaagcggc gaactacctc tgaagattgt tcgatcggtt cggaagctct
     3361 tacgtagtag ccgtgcgtga tctccttccg tttcagcaca ccgacgtcac gcgtcagcag
     3421 cagcctttgg tcggatgcag agatccgtgc gatctcatca tcggcaaaat cgttctgaaa
     3481 gagcgtgtcg aaaccgagca tacgcagatc acgtgcgaga cctccaaggt tcgagtcgac
     3541 gacgaagcgc gtatcacgaa gaggctgcgg cctaagccgg aggatcgggc ggatgtcgaa
     3601 agattcgaac atcgggtaaa cgctgatcac gtcgtcgttt ccaggacgat gatcgaatgc
     3661 aactgag
//
LOCUS       DATMED010000170         3143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_133732, whole genome shotgun sequence.
ACCESSION   DATMED010000170 DATMED010000000
VERSION     DATMED010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133732"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VNP73_04340"
     CDS             complement(<1..578)
                     /locus_tag="VNP73_04340"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HWL65185.1"
                     /translation="MKLPPAMLGVVAGVLLGVVSRMSDLLAPEVGWIGNLFAPWLAVA
                     FVLGRRAPIRIHAAGAGAAALVIAAVTHYAILRLSEHGLDAELFRHPVPLWGAIGAVG
                     GGIFGFLGWLSLEQGKSSVAAAAVLGASFFAEAAFITTVGRDAAFDLAVPLAVLAGVV
                     APLLALPSRKRAFLAVLLALVLVPFGLRGIGLL"
     gene            complement(575..883)
                     /locus_tag="VNP73_04345"
     CDS             complement(575..883)
                     /locus_tag="VNP73_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65186.1"
                     /translation="MSDPNNPLGPQGTSSRWDGNAKALFVMHILVSVLLASRLGNLWS
                     AYGFGLFIGCFVPPLVIALVLRWIYVAARSGRQPIWGTALLGMALFIDALLLLGFQAS
                     "
     gene            1010..2035
                     /locus_tag="VNP73_04350"
     CDS             1010..2035
                     /locus_tag="VNP73_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65187.1"
                     /translation="MNRDFLRGVVVATLASLFLHALVPVGGPGPARVQRRGHQVARLG
                     PWAVPRDHRERNLDLATLTIDRARIRVRPAPAGPEPKTEAPRLAAYRGLGSWIDIFNT
                     WVWDHPWATVSKMHDRGVETIYLQTATHGSTNPVVHPKVTKQFLLAAHRHDMRVVGWY
                     VPSFARPGRDYRRSLFGLRFGAKKHGFDSFALDIEATNVDNIHKRNVRLLWMAKRLRR
                     AAAPGYPLGAIIPDSHSLYWPDFPYKRVASIFDVMVPMGYFTFRAEGYKHVQRYTENN
                     IRTIRKETGRKTPIHVIGGLADEVGVPAARGFAKAVRKKRVLGASLYDYPITDDKTWD
                     ELAERIP"
     gene            2048..2806
                     /locus_tag="VNP73_04355"
     CDS             2048..2806
                     /locus_tag="VNP73_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWL65188.1"
                     /translation="MRRQVLAGLLIVGLLPSSPGVKAQEEWSPGMRAAIRYSSTRSGA
                     ISFAVKTPSGRLHAYRGHRVVPAASVFKVMLMVGYLRQGDVRDRKLNDSDRSLLEPMI
                     KRSDNSAASRIADQLGPRRIYRLARDAKMKHFRYTRPWGLSSIDATEQARFMFHLERY
                     LPRRHEDYARYLLSHIVKSQRWGIAKVRRPDWKLYFKSGWGSGTGAVSHQVAFLTHDG
                     LRIAVAIMIESSPSHAYSTETLRGIATRLLRELP"
     gene            2833..>3143
                     /locus_tag="VNP73_04360"
     CDS             2833..>3143
                     /locus_tag="VNP73_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65189.1"
                     /translation="MKILIVDDVPDARFILRKRLERERVFEVVGEAANGQEAVDLIAE
                     KTPDYVIMDFRMPVMDGLEATKIIRERFPAVQVIGFTAYEDPGMRESMLSAGAVTNVT
                     KT"
BASE COUNT          623 a         1001 c         1008 g          511 t
ORIGIN      
        1 agcagaccga tcccacgcag accgaacggg accagcacca aggcgagaag cactgctagg
       61 aaggcacgct tacgtgaagg aagtgccaga agaggagcga cgactccggc gagcaccgcc
      121 agcggcacgg cgagatcgaa cgccgcatcg cgtcctaccg tcgttatgaa ggcggcttct
      181 gcgaagaacg aagcacccag cactgccgcc gccgcgaccg aggacttgcc ctgctcgagc
      241 gacagccatc caaggaaccc gaatatccct ccaccgaccg cgccgatggc accccagagc
      301 ggaacggggt ggcggaacaa ctcggcatcg agtccatgct cggacaatct caggatggcg
      361 tagtgagtga ccgctgcgat caccagtgca gctgcccccg ctccggccgc gtggatgcgg
      421 atgggggctc ttcggcccaa cacgaacgcg accgccagcc acggggcgaa caggttgccg
      481 atccacccga cctcgggtgc gaggaggtcg ctcatcctgc tgacgacgcc gaggagaacc
      541 ccggcgacga cgccgagcat ggccggtgga agcttcacga tgcctggaaa cccaacagca
      601 ggagcgcgtc gatgaacagc gccatgccca gaagggccgt accccagatg ggctgccggc
      661 ccgaccgggc cgcaacatag atccatcgca ggaccagggc gatcaccaaa gggggcacga
      721 agcacccgat aaagagcccg aacccgtagg cggaccatag gttccccaac cgagacgcaa
      781 gtagcacgga caccaagatg tgcataacga agagggcctt cgcgttcccg tcccagcgcg
      841 acgaggttcc ttggggcccg agagggttgt tggggtcgga catccccggc ttcatacccg
      901 aggcgttcgc tcgggttcct accttacgta gtgcatgttc ggtcgtgcct ccctagcgtg
      961 gttgcttgac tgcgaggcgt aaaccgatgc aaaccctgag ggagcggcaa tgaaccgcga
     1021 tttcttacgt ggagtcgttg ttgccactct cgcgtccctc ttcctccacg cactggtgcc
     1081 ggtcggtggc cccgggccag cccgcgtgca gcgtcgtggt catcaggttg cccggctcgg
     1141 gccctgggcg gtcccgcggg accatcggga gcggaatctg gacctcgcga cgctaacgat
     1201 cgaccgggcc cggatcaggg tgaggccggc tccggccggc cccgagccga agacggaggc
     1261 tcctcgcctt gccgcctacc ggggcctcgg atcctggatc gacatcttca acacgtgggt
     1321 ctgggatcac ccctgggcga cggtgtccaa gatgcacgac cgcggcgtgg agacgatcta
     1381 cctgcagacc gcaacccacg gcagcacgaa cccggtggtg catccgaagg taacgaagca
     1441 attcctgttg gctgcacatc gtcacgacat gagggtcgtc ggttggtacg tcccgtcgtt
     1501 cgcccgaccc ggacgcgact acaggcgatc gctcttcggc ctccgcttcg gtgccaagaa
     1561 gcacggtttc gactcgttcg ccctggatat cgaggcaacg aacgtagaca acatccacaa
     1621 acgcaacgtc cgcctgctgt ggatggcgaa gcggctgagg cgggccgctg ctcccggcta
     1681 cccactgggg gccataatcc cggactcaca ttcgctgtac tggccggact tcccgtacaa
     1741 gcgggtcgcg tcgatcttcg acgtgatggt gccgatgggc tacttcacgt tcagggccga
     1801 gggttacaag cacgtccagc gctatacgga gaacaacatc cgcacgatcc gcaaggagac
     1861 cgggcgcaag acgccgatcc acgtgatcgg tggtctggcg gacgaggtcg gggttccggc
     1921 cgcccgcggc ttcgccaagg cggtgcgtaa gaagcgggtt ctcggcgcca gcctctacga
     1981 ctacccgatc accgacgaca agacgtggga cgagctggcc gaacgcatcc cctgacataa
     2041 tccctggatg cgcagacagg ttctggcggg cctgctgatt gtgggtctgc tgccttcatc
     2101 tcccggtgta aaggctcagg aggagtggtc gcccggcatg cgggcggcga tccgatactc
     2161 gagcacccgc tcgggtgcga tcagcttcgc cgtgaagact ccgtcgggga gactgcacgc
     2221 ttatcggggt catcgcgtcg ttccggccgc gagcgtgttc aaggtcatgc tgatggtcgg
     2281 ctacttacgt caaggcgacg tgcgcgatcg gaagctcaac gactcggatc gttccctgct
     2341 cgaaccgatg atcaagcgtt cggacaactc agcggcctcc cggatcgcag accaactggg
     2401 cccccgccgg atctaccggc tcgcacgcga tgcaaagatg aagcatttcc gctacacgag
     2461 accgtggggc ctatcgagca tcgatgccac ggagcaggct cgcttcatgt tccatctcga
     2521 gcgctaccta ccgaggcgcc acgaggacta cgcgcgctac ctgctgtcac acatcgtcaa
     2581 gtcgcagagg tgggggatcg cgaaagttcg caggccggat tggaagctgt acttcaagag
     2641 cggttggggt tcgggcacgg gagccgtctc gcaccaggtc gcgttcctta cgcacgacgg
     2701 cctcaggatc gccgtagcga tcatgatcga gagctcgccc agtcacgcct actccaccga
     2761 aaccttgaga gggatcgcga cccgcctgct tcgcgagctg ccttaaaagc aggtggtggt
     2821 ttactcatgc cgatgaagat cctgatagtt gacgacgtgc ctgatgcacg tttcatcctg
     2881 cgcaaacgac tcgagcgcga gcgggtgttc gaggtggtcg gcgaggcggc gaacggccag
     2941 gaagccgtcg atctgatcgc cgagaagacc cccgattacg tgatcatgga cttccggatg
     3001 ccggtgatgg acggcctgga ggccacgaag ataatcagag agcgcttccc ggcggtgcag
     3061 gtgatcggct tcaccgccta cgaggacccc gggatgaggg aatcgatgct gtcggccggg
     3121 gccgttacga acgtgacgaa aac
//
LOCUS       DATMED010000171         4320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_134106, whole genome shotgun sequence.
ACCESSION   DATMED010000171 DATMED010000000
VERSION     DATMED010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134106"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VNP73_04365"
     CDS             complement(<1..404)
                     /locus_tag="VNP73_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWL65190.1"
                     /translation="MAEVKLQIEKRDGTGKGVARRARADGKVPGVVYGRGMEPVSVVV
                     DRREFVTALHTDAGMNVLLSLQLDGESIPALARELQRDPVKGTLLHVDLVKIDLKQEV
                     EVDVPVHVVGEAPGVKEGGVLEQPLHQVHVRC"
     gene            471..827
                     /locus_tag="VNP73_04370"
     CDS             471..827
                     /locus_tag="VNP73_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWL65191.1"
                     /translation="MEHAAEMGAEQFCYVTTKGRRTGRPHTIEIWFGVVDRTLYLLSG
                     GGMRSDWVANLVAAPDVSVRVGSTEYPARARIVDDPDEEARARPLLAGKYLGWKEGEA
                     MSGWAQTALVIALDLA"
     gene            complement(831..1820)
                     /locus_tag="VNP73_04375"
     CDS             complement(831..1820)
                     /locus_tag="VNP73_04375"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWL65192.1"
                     /translation="MQLPTQKRLMLFSGTASRELAAEIVAHLGMKLGHVEIEEFANSE
                     TYVRFDDSVRGCDAFVIQSHSAPVNHHLMQQLLMIDALKRASAKRITAVSPFYPYSRQ
                     DKKTRSREPITSKLVADFYEAAGVDRVLSVDLHTGQIQGFFDVPFDHLTALPLLADYF
                     AHMLEDDFVIVSPDAGRVRLAEKWTQHLHDFHDLNGSVAFLHKKRQGDARNVSKTLAV
                     VGEVEGRVCIIVDDMIDTAGTLVNGAEALVAAGATEVYAAATHPVLSDPATDRIKNSP
                     IHQVVVTNTLPIPSEKQLDKLVILSIAPTIAQTIKAVFEEESVSELFHGENQP"
     gene            complement(1846..3285)
                     /gene="glmU"
                     /locus_tag="VNP73_04380"
     CDS             complement(1846..3285)
                     /gene="glmU"
                     /locus_tag="VNP73_04380"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWL65193.1"
                     /translation="MVAGDRYGVVLAAGEGKRLRSSVPKVLHRVAGRSLVGHVLSAVS
                     GVGLDRVVVVVSANTEAFTEALEADGLGMDVELVVQDPPAGTADAVRIALDHIGSAAG
                     SALIVAGDTPLIDGNTLGTLLGEHDERGSSVTLLTADVENPAGYGRIVRSEDGSVDRI
                     VEDADATDEERSLAEINSGTYVFDLERLTEMIAKVDKENRQGEYYLTDMVELLRSAGD
                     GIGAVLASEEEVRGVNTRVQLASVREALNRKTCEALLEQGVGIVDPSTTYIDVTVEIA
                     PEAVILPFTFLEGRTRIGERAEVGPQTRIVDSEIGPDAEVSFAVVRDSIVGPEASVGP
                     FASLRPGTRLERGARAGTFVETKKTTIGEGSKANHLAYLGDAEIGRGVNVGAGSITCN
                     WDGKEKHKTIIEDDAYIASDTMLVAPTRIGKRAATGAGSVVRGDVPDDALAVGVPARI
                     IEGKGDKLGRKPGKKSPPETEGDAGGAGQ"
     gene            complement(3307..3378)
                     /locus_tag="VNP73_04385"
     tRNA            complement(3307..3378)
                     /locus_tag="VNP73_04385"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3343..3345),aa:Gln,seq:ttg)
     gene            complement(3397..>4320)
                     /locus_tag="VNP73_04390"
     CDS             complement(3397..>4320)
                     /locus_tag="VNP73_04390"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWL65194.1"
                     /translation="HGPMNPTVDAATSLRIRTNSKLNLYLRVVGRRPDGYHELESIFH
                     GIGLGDEIELEPTSTGNTEVEMELGDLAGEIPTSEENLVFQAATRLVERGAMNPGVRI
                     KIKKNIPIAAGLGGGSGNAAGALFALSKLWGLELPDTDMLEVAGLIGSDVPYCIGGGT
                     VLAMKRGEELTPLPAPHRLWFVLGVSFRKMLTRDVYEEWDRVDPVDNAGSAPLTFALG
                     VGNAAEVAMLLHNDLEQAAFRLWPELGSKKQALIDAGALGAAMTGSGPTIFGVANDSD
                     HAHEIAARIKGDFDRALVVSSHPRCVEWVDS"
BASE COUNT          762 a         1408 c         1334 g          816 t
ORIGIN      
        1 atgcagcgaa cgtgcacctg gtgaagcggc tgctccagaa cgccaccttc tttgacaccg
       61 ggagcctcgc cgacgacgtg caccgggacg tcgacctcga cctcctgctt gaggtcgatc
      121 ttgaccaggt cgacgtgaag aagcgtcccc ttgacggggt cgcgctggag ttccctggcc
      181 agagccggga tcgactcgcc gtcgagttgc agggagagaa gcacgttcat gccggcgtcg
      241 gtgtggagag ccgtaacgaa ctcgcgccga tcgacgacga ccgagacggg ctccatcccg
      301 cggccgtaga ccacgccggg gaccttgcca tcggcccgag ccctgcgagc gacaccctta
      361 ccggtgccgt ctctcttctc gatctgcaat ttgacttcgg ccacgctgaa ctcctggttt
      421 tgataaggat cggggagcta gaagcccgcc cggcgagcca ttataggggg atggagcacg
      481 cggctgagat gggggccgaa cagttctgct acgtgaccac gaagggcaga cggaccggca
      541 ggccgcacac gatcgagatc tggttcgggg tcgtcgaccg cacgctttac ctgttgtcgg
      601 ggggagggat gcgctcggac tgggttgcca atctcgtcgc ggcacccgac gtgagcgtga
      661 gggtgggctc gaccgaatac ccggctcgag ctcggatcgt cgacgacccg gacgaagagg
      721 ctcgtgcccg tcccctgctc gcagggaaat atctgggctg gaaagagggc gaagcgatgt
      781 cgggctgggc ccagaccgcc ctggtgatag ccctggacct cgcttagggg ttagggctgg
      841 ttttcgccgt ggaagagctc ggacacggac tcctcctcga acacggcttt gatcgtctgg
      901 gcgatcgtcg gcgcgatcga gaggatcacc agcttgtcga gctgcttctc gctggggatc
      961 ggaagcgtgt tcgtcacgac tacctgatgg atcggtgagt tcttgatccg gtcggtggcc
     1021 gggtcggaca gaacggggtg cgtggcggcc gcgtagacct cggtcgcacc cgctgcgacc
     1081 agagcctccg cgccgttcac gagcgttccg gccgtatcga tcatgtcgtc gacgatgatg
     1141 caaaccctcc cctcgacctc accgactaca gccaatgtct tcgagacgtt gcgcgcatcg
     1201 ccctgacgtt tcttgtgaag gaacgcgacc gagccgttca gatcgtggaa gtcgtggaga
     1261 tgctgggtcc acttctccgc cagacgcacg cggcccgcat ccggcgacac gatcacgaag
     1321 tcgtcttcga gcatgtgtgc gaagtagtcg gccagcaacg ggagagccgt cagatggtcg
     1381 aacggcacat cgaagaagcc ctggatctga ccggtgtgca ggtcgaccga gagaacgcgg
     1441 tccactccgg cggcttcgta gaagtcggcg acgagtttgg aggtgatcgg ctcgcgcgag
     1501 cgcgtcttct tgtcctgacg cgagtacggg tagaagggtg aaacggcggt gatgcgtttg
     1561 gccgaggccc gcttcagcgc gtcgatcatc agtagctgct gcatgaggtg atggttcacg
     1621 ggggccgagt ggctctggat aacgaacgca tcgcatccac gcaccgagtc atcgaaacga
     1681 acgtaggtct cagagtttgc gaactcctcg atctcgacgt ggccgagctt catgccaagg
     1741 tgggcaacga tctcagctgc gagctcccgg ctggcggtgc cggagaagag catcagtcgc
     1801 ttctgagtcg gaagttgcat gttcctcctt agtccctact ccactctatt gtccggctcc
     1861 ccctgcgtct ccttccgtct ccggaggaga tttcttgccc ggtttccggc ccaacttgtc
     1921 acctttgccc tcgatgatcc gtgcgggcac gccgaccgca agcgcgtcgt cgggaacgtc
     1981 cccccgcacg acactgccgg cgccggtggc ggctcgcttg ccgatcctgg tcggcgcaac
     2041 gagcatggtg tccgaggcga tgtaggcgtc atcctcgatg atcgtcttgt gtttctcctt
     2101 cccatcccag ttgcaagtga tggaaccggc gcccacgttc actccacgcc cgatctccgc
     2161 atcgcccaga taggcaaggt ggttcgcctt gctaccttcg ccgatagtcg ttttcttcgt
     2221 ctcgacgaag gttccggcgc gcgcaccacg ctcgagacga gtgcccgggc gcagtgaggc
     2281 aaaggggccg accgacgctt ccgggccgac gatcgagtca cgcacgaccg cgaacgacac
     2341 ctctgcatcg gggccgatct cggaatcgac gatccgcgtc tgcgggccca cctcggctcg
     2401 ctccccgatg cgcgtgcggc cctctaagaa tgtgaagggg aggatcaccg cttccggagc
     2461 tatctcgacc gtgacgtcta tgtaggtcgt cgacggatcc acgatcccca caccttgctc
     2521 cagcaacgcc tcgcacgtct tccgattcaa tgcctctcga acggaggcga gctggacgcg
     2581 ggtgttcacc cctcggacct cttcttcgga tgcgagcacc gcaccgatgc cgtcccccgc
     2641 ggaccggagt agctcgacca tgtcggtcag gtagtactcg ccctggcgat tctccttgtc
     2701 gaccttcgcg atcatctcgg tcaatcgctc gaggtcgaag acgtatgtcc cggagttgat
     2761 ctcggccagc gatctctctt cgtccgtggc gtcggcatcc tcgacgatcc gatcgaccga
     2821 cccgtcctcg ctccgcacga tgcgtccgta tccggcgggg ttctcgacgt cggcggtcag
     2881 caaggtcacg ctcgatccgc gctcgtcgtg ctcgccgaga agtgtgccga gggtgttccc
     2941 gtcgatcaaa ggcgtatctc cggcaacgat cagagcggac cccgccgcag atccgatgtg
     3001 gtcgagcgcg atgcgaaccg cgtcggcggt acccgccggg ggatcctgaa ccacgagttc
     3061 gacgtccatg ccgaggccgt ccgcttcgag cgcttcggtg aaagcctcgg tgttcgccga
     3121 cacgacaacc acaacccgat ctagccccac gcccgatacg gccgacagca cgtggccgac
     3181 cagggaccgg ccggccaccc gatggagcac cttcggcaca ctcgaacgaa ggcgtttccc
     3241 ctcgccggca gcgaggacta cgccgtatcg atcacctgct accaagagac ctcccggcag
     3301 ctcgcgctgc cccgcctgga ctcgaaccag aacttaagga tccaaaatcc ttcgggctgc
     3361 caattacccc acggggcacc atcacgtcat tgaatcctat gagtcgaccc actcaacgca
     3421 gcgcgggtgg gacgacacca cgagggctcg gtcgaaatcg cccttgatcc gagcggcgat
     3481 ctcgtgggcg tggtctgagt cattggctac gccgaagatc gtggggcccg acccggtcat
     3541 agccgccccg agggcccccg cgtcgatcaa cgcctgcttc ttagaaccca actcgggcca
     3601 cagtcggaaa gcggcttgct cgagatcgtt gtgcaacagc atcgctacct ctgctgcgtt
     3661 cccaaccccc agcgcgaatg tgagcggagc actgcccgcg ttgtcgacgg gatcgaccct
     3721 gtcccattct tcgtaaacgt ctcgggtcag catcttgcgg aaggaaaccc cgaggacgaa
     3781 ccacaggcga tggggtgcgg gaagaggcgt tagctcttcc cctcgtttca tcgccagcac
     3841 cgttccacct ccgatgcaat aaggcacgtc actgccgatg agacctgcaa cttccagcat
     3901 gtcggtgtcc gggagctcaa gaccccacag cttgctcaac gcgaacagcg ccccggcagc
     3961 gttaccgctg cctcctccaa gacccgctgc gatcgggatg ttcttcttga tcttgatcct
     4021 gacgccggga ttcatcgccc ctcgttcgac caaccgtgtt gcagcctgga agaccaggtt
     4081 ctcctccgac gtggggatct cgccggcgag gtcacccaat tccatctcga cctcggtgtt
     4141 gccggtcgag gttggttcga gctcgatctc gtcgcccaga ccgatcccgt ggaatatcga
     4201 ctcgagctcg tggtacccgt cggggcggcg gccgaccacg cgcagataca gattcaactt
     4261 cgagttcgtc cggatgcgca ggctggtggc ggcgtcgacg gtagggttca tgggcccatg
//
LOCUS       DATMED010000172         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2444067, whole genome shotgun sequence.
ACCESSION   DATMED010000172 DATMED010000000
VERSION     DATMED010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2444067"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..884)
                     /locus_tag="VNP73_04395"
     CDS             complement(195..884)
                     /locus_tag="VNP73_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL65195.1"
                     /translation="MSHSVLLAAQGLVKSYRRGPEEIHALAGATFDLYPGEVIALIGP
                     SGSGKTTLLNLIVGWEDAESGSIEWSGQHVPAADRLWNDLSIVPQRLGLIEELSVAEN
                     IRLPLRLGQKNSVGDEAQSLMDDLGLAQFADRLPTEISLGEQQRTGLARALVSTPRLL
                     LADEPTGHQDAVWGRGVLRTLRRAADAGTTSLIATHNEEVLKYVDRVLAIKDGHLHEE
                     DVGQGRREDAH"
     gene            complement(881..1726)
                     /locus_tag="VNP73_04400"
     CDS             complement(881..1726)
                     /locus_tag="VNP73_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65196.1"
                     /translation="MQTESIAASALGVVKIYWTATGEVHALKGVDALFSAGQVTALVG
                     PSGSGKSSLMRILCGLDRPTAGSVMVGRTEVSALKGAKLHRFRRDHVGYVFQRPSDNL
                     IPYLTAREHLEHSARLKGLGETSKVDELLDQLGIANRDHHRPHQLSGGEQQRLAFAQA
                     VIGDPTIVVADEPTAELDSESSHSLLETVKDLAHRGIGVVVSTHDPAVVDVAERTIYL
                     RHGAVEAETTEARNLSVIDAAGRVQLPPEILKLFPNRRAVMTVEGSEVKLIPPDSIDW
                     EQGQS"
     gene            complement(1716..>3452)
                     /locus_tag="VNP73_04405"
     CDS             complement(1716..>3452)
                     /locus_tag="VNP73_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWL65197.1"
                     /translation="VALIVFSAAGIFAVQRRRIEVRLLSSQGVSPLSQGLRGAVEALI
                     PALLGVALGWGVAVYLVRAIGPTSSLSEGAEGQALATALWSCALAIALYGAVTMVSAR
                     REVEVGAARFRGLVARFPWEPIVLVLAGASYYEIVTRGEPIVDSADGALRVDMLLLLF
                     PILFIIGMSGVAARGLRRLLPRVRRMGDRSAPSMYLASRRLAGASRIALVLITASSIS
                     LGLLVYASILVASNEASAHAKANLSVGSDVAVPVQTSQPNNLLGTTFDFPFTFVTRTD
                     DGDIQPGDREFETLGIDPEAFADIAYFDPSFASESIDELVGRLEPFDGGALTAILVAA
                     EEEVPDGAVYVTPRYDVPIQIVGTASAWPGQSADKPMLVTRYDALFGVSAASESSVSE
                     QLVTDYELWARAPAAEVEQALLAERINFNPVSVLSTEEILTSPSFLALTWTFGYLQAL
                     GVMAGAIAMAGMLLYLQTRQQAREVSYALARRMGLTRSAHRWAVALELGGMLVISLLI
                     GAVLAVASSWLVYARLDPLPAIPPGPLFAVPPLLFVVLIALISLAALLGAWRVQRKAD
                     SAKVSEVLRYAN"
BASE COUNT          672 a         1106 c         1085 g          589 t
ORIGIN      
        1 tcccttcctg cttcttcatc tcacgcttgt actgcctgtt cacgagagcc ctttcctcgt
       61 actcgagccg ggtcgttcca ttccctcgcc gtcgccgaag cgcgctgtag aagggtccgg
      121 tcgggggact tgaaacgagc cacaagacta gcatgggccg gacccgcagc gagggggccg
      181 tccgtgtatg ccgcctagtg cgcgtcctcc cgacggccct gacccacgtc ttcttcgtgc
      241 agatgaccgt ccttgatggc gaggacccgg tcgacgtatt tgagaacctc ttcattgtgg
      301 gtcgcgatga gcgaggtcgt gccagcatcg gcggctcgcc ggagcgtcct cagcactccc
      361 cgcccccaga ccgcgtcctg atgaccggtc ggctcgtcgg cgagcagtag gcgaggagtg
      421 ctgaccaagg cccgggcaag ccccgttcgc tgctgctcgc cgagcgagat ctcggtgggg
      481 aggcggtcgg cgaactgagc caaccccagg tcgtccatca ggctctgcgc ctcgtccccc
      541 accgagttct tttggcccaa acgaagggga agacggatgt tctcggccac gctcagttct
      601 tcgatgaggc ccaagcgctg ggggacgatc gataggtcgt tccacagcct gtcggcggcg
      661 ggcacgtgct gtccgctcca ctcgatcgaa ccggattcgg cgtcctccca cccgacgatg
      721 aggttcagga gcgtcgtctt gcccgaaccc gacgggccta ttagggcgat gacctcgccg
      781 ggatagagat cgaacgtggc accggcgagg gcatggatct cctcggggcc gcgccggtaa
      841 ctctttacca agccttgcgc ggccagaagc accgagtggc tcacgactgg ccttgttccc
      901 agtcgattga atccggaggt atcaatttga cctcgctgcc ctcgacggtc atcacggcgc
      961 gccggttggg gaacagcttg aggatctcgg gtgggagctg cacccttccc gcggcatcga
     1021 tgaccgagag attgcgcgcc tcggtggtct cggcctccac cgctccgtga cggagataga
     1081 tcgtccgctc ggcgacatcg acgaccgccg ggtcgtgggt cgaaacgacg accccgattc
     1141 cccggtgggc gagatcctta accgtctcga gcaacgagtg actcgactcg ctgtcgagct
     1201 ccgcagtcgg ctcatccgca acaacgatgg tcggatcgcc aatcactgct tgagcgaatg
     1261 cgagccgctg ttgctcaccg cccgaaagct gatgaggccg gtgatggtcg cgattggcga
     1321 ttcccaattg gtcgagaagc tcgtcgacct tgctcgtctc ccccagaccc ttgagccgcg
     1381 cgctgtgctc gagatgctcg cgcgcggtta ggtaagggat gaggttgtcg gacggcctct
     1441 ggaacacgta gcccacgtgg tcgcgtcgga aacggtggag cttcgcaccc ttgagcgcgc
     1501 tgacctccgt ccggccgacc atcaccgaac ccgcggtggg tcggtcgagc ccgcagagga
     1561 ttcgcatgag tgaggacttg ccgctacccg agggaccgac gagggcagtg acttggccag
     1621 ccgagaacaa cgcgtcgact cctttgagcg cgtgaacctc tccggtcgcg gtccaataga
     1681 tcttcacgac cccaagtgcg ctcgcagcta tcgactcagt ttgcatagcg caaaacctcc
     1741 gacacctttg cgctgtcggc tttccgctgc actcgccacg cgccgagcaa ggcagccaga
     1801 gagatgaggg ctatcaagac aacgaagagc agcgggggga cggcgaacag aggcccgggt
     1861 ggaatcgccg gcagtggatc caaccgtgcg tacacgagcc aagacgaggc cacagcaagt
     1921 acggctccga tcaacaacga gatcacgagc atgcccccca actcgagcgc gaccgcccat
     1981 cggtgggcgc tcctggtcaa ccccattcgc cgcgcgagag cataggaaac ctcgcgcgct
     2041 tgttgccggg tttggaggta caggagcatg ccagccattg caattgctcc agccatgacg
     2101 ccaagtgcct ggagatagcc gaaggtccat gtgagcgcca ggaaagaggg actggtgagg
     2161 atctcctccg tactcaggac gctcaccgga ttgaaattga ttcgctccgc gagaagggct
     2221 tgctcgactt cggcggcggg ggcccgcgcc cagagttcat agtccgtcac cagctgttcg
     2281 gacaccgagc tttcggatgc tgccgacaca ccgaagaggg cgtcgtagcg cgtcaccagc
     2341 atcggcttgt ccgcgctctg ccccggccag gcggatgcgg taccgacgat ctgaatgggg
     2401 acgtcgtatc gaggtgtcac gtacacggca ccgtccggaa cttcttcttc ggcggcgacc
     2461 agaatcgcgg tcaaggctcc gccgtcaaag ggttcgagtc gccccactag ctcatcgatg
     2521 ctttccgaag caaagctcgg atcaaaatac gcgatatcgg cgaatgcttc aggatcgatt
     2581 cccaatgttt cgaactctcg gtcacccggt tgaatatccc catcgtctgt ccgagtaaca
     2641 aacgtgaagg gaaagtcgaa ggttgtgccg aggagattgt tgggctgcga tgtctgaaca
     2701 ggcacggcca catcgctccc gaccgagaga ttcgctttcg cgtgggcgga tgcctcgttc
     2761 gaggccacga gaatgctggc gtagacgagc agtcccaaag agatggacga tgccgtgatc
     2821 aggacaagag cgatgcggga agctcctgca agccgccgac tggccaaata catggaggga
     2881 gcagaacgat cgcccatccg gcgaacgcgc gggagcagtc ttcgtagacc tcgagccgcg
     2941 acgccggaca tgccgatgat gaacagaatg ggaaacagca gcagaagcat gtcgaccctc
     3001 agggcaccat cggccgagtc cacgatgggt tcgcctcgcg tgacgatctc gtagtaggac
     3061 gcgccggcga gcaccaagac gatcggctcc cagggaaaac gcgccacgag gccccggaag
     3121 cgcgccgcgc cgacctctac ctcgcgtcgc gcgctcacca tcgtgaccgc gccgtagagg
     3181 gcaatcgcaa gggcacagga ccacagcgcg gtcgcgaggg cctgtccctc cgctccctcg
     3241 ctcaacgagc tcgtcgggcc gatcgcgcgg acgagataga cggcgacccc ccagccgaga
     3301 gccaccccga gcagcgccgg gatgagagcc tcgacggcgc ctcgcagtcc ttgactcagt
     3361 ggggagacac cttgagaaga caagagtcgc acttcgatcc ggcgcctttg caccgcgaag
     3421 atgcccgccg cagagaagac gataagggcc ac
//
LOCUS       DATMED010000173         6101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3579251, whole genome shotgun sequence.
ACCESSION   DATMED010000173 DATMED010000000
VERSION     DATMED010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3579251"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VNP73_04410"
     CDS             complement(<1..202)
                     /locus_tag="VNP73_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWL65198.1"
                     /translation="MEWRILRLGIKRTKEDEMSIPESHRDILDHPKSFAHWATVGPDG
                     APQVNPVWFEFDGEQLVISQTKT"
     gene            complement(221..1516)
                     /locus_tag="VNP73_04415"
     CDS             complement(221..1516)
                     /locus_tag="VNP73_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HWL65199.1"
                     /translation="MMRRLPVIALATMLVAALNFSPVGAKEKRGLEVVNQKALSKRLI
                     EYTVESPAMGAETKLRVLLPSGYSSKADRDYPVVYLLHGCCDDFRSWSDKTDLTKHTK
                     AMDLVVVMPDAGNGGWYTDWYSGAFNWESYHIGELIPWVEKTFDVLPAREGRAIAGLS
                     MGGFGAFTYASRHPDLFVAAASFSGAVDSNFQEGAAVDAVAGLDGGKPGDVFGPRATD
                     EIRWRGHNPWDLAENLSTVDVTLRTGNGESSSGIDPLERGCWEMSTNVHNRLKELGQK
                     HIWDDYGPGTHTWPFWERDLHKTLPRYLSLFEDPPPAPKSFTFRSMDAAYDVFGWNVQ
                     LKRPVLEFSKLVVRGSDGFKLTGSGSGSVSTAAIYKPGEKYFVTVGKKTSIVDADDRG
                     RLKIAVDLGAANAFQAFTAPARATSAEHTVTVAISPLRS"
     gene            complement(1523..2467)
                     /locus_tag="VNP73_04420"
     CDS             complement(1523..2467)
                     /locus_tag="VNP73_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWL65200.1"
                     /translation="MNDGGGISRRKFLGAGAIAGGGTILGASNAFAGTSGLDVKGTPF
                     SNYTGDPYLPKNLIGLMLYTVRDQVDSVGFAEVFETVAGMGYTAVEFAGYTQGTGDIT
                     PKQIRKLMEANGLVGVGSHGNLDDAAIEAAVALDMPYTGTAFEIPRDTSTAGWEQLAA
                     DYNAFGQKAAKHGVRGYLHIHGPAYGPVADNPDIRGLDILLEQTDPKFFAFEMDIYWA
                     YFFRQTFPGYDPITWVKDNRDRFPLFHVKDGIHDGGEPDFRDVGQGNVPFEEFFKSVK
                     GARKSWYLNERDSAADHPRGSFCSAQASYLYMRYGLRK"
     gene            complement(2479..3435)
                     /locus_tag="VNP73_04425"
     CDS             complement(2479..3435)
                     /locus_tag="VNP73_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWL65201.1"
                     /translation="MSQFRGVEQRSISRRGFLGAAAGVASLAALKTMPGVAWAASDCT
                     AKVKDSQIGIQLFTCTSMYLANTPLLLERLAAIGYQKVEHALGWGTTGSPEAFRQACE
                     DVGITATSGHYGDGLHPYDPDTWKQTLENANIVGQTYVGVASKPSGDNSKAAWMAYAE
                     DISKAAPVAREAGFDQVFHHCHDGEWKPVDDDPKVRPADIMLKYTDPDLFHVQLDIGW
                     AYNGLGSVDAVCKELRRYKGRFRTVHVKDFRAGAPVQPGTGEIGAEGFAKIFGAAKET
                     RQNITEFHVESDSSVVTCYDTAQLGWDLLHDMEYSYNCSKRS"
     gene            complement(3632..4654)
                     /locus_tag="VNP73_04430"
     CDS             complement(3632..4654)
                     /locus_tag="VNP73_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWL65202.1"
                     /translation="MKQLRIFGIALLAVMLLLGACTDDGGGTTEGAQPAADAGDDNPE
                     GRTVKIAFSAPGADHGWLAAITENARAEADKYDDVELVLNEGTNDSAAQASQVETLVN
                     QDPDVLVILPHEGDALTPAAQDAMAAGIPVVNLDREFSSPSAYRTWIGGDNYGIGKSA
                     GDYFAQELDCKGNVVEIQGLAGISVTELRTKGFADAIKQCDGGIKVIAQQPADFLPDK
                     GLEVMEAILQSEDQIDAVYTHDDDMAMGVVSAIQNAGREGEMFVTGAGGSADAMKLIQ
                     EGDLYKATFLYNPSMSASAIRLARLIALGAPMGDLVEPEVPSYIQLPATTVTQENVDD
                     VIDLGY"
     gene            complement(4670..5644)
                     /locus_tag="VNP73_04435"
     CDS             complement(4670..5644)
                     /locus_tag="VNP73_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65203.1"
                     /translation="MTDAVTRNDQTAADDRTRRRIYIQEYALVGVLIVLIVAGSIAKP
                     SAFLSSSNLFTVLTQASVVGVLAIGMTFVIASSGIDLSVGSLVAAAGIAGGLLGDGGS
                     GMAFILGALGFGLLLGSINGFAITFGRVVPFIATLAMLTIGRGLALRMSGKTPISVIL
                     LDQVRWFGNGKVLGIPAPVIVLIGLTAIGWVVLNRTAYGRYVVAIGGNREAARIAGIR
                     VRPIVFSVYALSGLCAGISAILITGRLASASPVAGALYELDAIAAVVIGGTSLAGGRA
                     TMVGTFLGVMTFALIFNLLNLLNIPTEYQLIVKGVIVLIAVVAQRRDS"
     gene            complement(5641..>6101)
                     /locus_tag="VNP73_04440"
     CDS             complement(5641..>6101)
                     /locus_tag="VNP73_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL65204.1"
                     /translation="NVSLADLKRFRRGLLLNIGEERKRAGEQLDAIGTEPGSTDRQVR
                     ELSGGNQQKVVLARWLLRAPGVLFLDEPTRGVDVGAKTEIYRVVAELAEKGLGILMVS
                     SEFPELLGFCDRILVTRDGRIVREAKGGEISEEEVLSLVVRREPVAEVAS"
BASE COUNT         1134 a         1968 c         1829 g         1170 t
ORIGIN      
        1 gcgtcttcgt ctgactgatg acgagctgct ccccgtcgaa ctcgaaccac acggggttca
       61 cctgcggcgc accgtcgggt ccgaccgtgg cccagtgagc gaaactcttc gggtggtcga
      121 ggatgtctcg atggctctcc ggaatgctca tttcgtcctc cttagtcctt tttatcccca
      181 accggaggat cctccactcc attccctggg aggagcggag ctaggaacgg agcggagaga
      241 tcgcaacagt gacggtgtgc tcggcgctcg tcgcacgagc cggagccgtg aatgcctgga
      301 acgcgttggc ggctcccagg tccacagcga tcttcagccg gccgcgatcg tcggcgtcga
      361 cgatcgatgt cttcttcccg accgtgacga agtacttctc gcccggcttg tagatcgcgg
      421 cagtcgacac cgagccgctg cccgagccgg tgagcttgaa tccatccgaa ccacggacca
      481 cgagcttcga gaactccaac accgggcgtt tcagctgcac gttccagccg aagacgtcgt
      541 aagccgcgtc catggatcgg aaagtgaacg acttcggcgc gggcggtgga tcctcgaaca
      601 gcgacaggta tcgcggcagc gtcttgtgaa ggtcgcgctc ccagaagggc cacgtgtgag
      661 tcccgggtcc atagtcgtcc cagatgtgtt tctgcccaag ttccttgagt cgattgtgaa
      721 cgttcgtcga catctcccaa caaccccgct cgagcgggtc gatgccgctg gagctctcac
      781 cgttgcccgt tcgtagggtg acgtcaaccg tcgacaggtt ctcggcgaga tcccaaggat
      841 tgtgccctcg ccagcggatc tcatccgtgg cgcgggggcc gaatacgtca ccgggcttgc
      901 ccccatcgag gcccgcgacc gcgtcgaccg ccgctccttc ttggaaattg gagtcgacgg
      961 cccccgaaaa cgatgccgcg gctacgaaaa ggtcgggatg ccgcgatgcg taggtgaacg
     1021 ccccgaatcc ccccatcgag agaccggcga tcgcacgccc ttctcgtgcg ggaagcacgt
     1081 cgaacgtctt ttccacccag gggatcagtt caccgatgtg gtagctctcc cagttgaagg
     1141 cgccgctgta ccaatccgtg taccagccgc cgttgcctgc gtccggcata acgaccacga
     1201 gatccatcgc cttggtgtgc ttggtcagat ccgtcttgtc ggaccacgat cggaagtcgt
     1261 cacaacaacc gtgcaggagg tagaccacag ggtagtcacg atccgctttc gatgagtagc
     1321 ccgagggaag gagaacgcgt agcttggtct cagcccccat cgcgggactc tctaccgtgt
     1381 actcgatgag gcgcttagaa agcgcctttt ggttcacgac ctcgaggccg cgtttctcct
     1441 tagccccgac gggcgagaag ttgagtgccg cgaccaacat ggtcgccaga gcgatgaccg
     1501 gtaaccggcg catcatgctt ggttacttgc ggaggccgta gcgcatgtaa aggtagctgg
     1561 cctgcgccga gcagaacgaa ccacgtggat ggtccgccgc gctgtcccgc tcgttgaggt
     1621 accagctctt cctcgcgcct tttaccgact tgaagaactc ctcgaacgga acgtttcctt
     1681 ggccgacgtc gcggaaatct ggttcgcccc cgtcgtggat cccgtccttg acgtggaaga
     1741 gggggaagcg gtcacggttg tctttgaccc acgtgatcgg gtcgtaaccg ggaaaggtct
     1801 gacggaagaa atacgcccag tagatgtcca tctcgaaggc gaaaaacttc ggatccgttt
     1861 gctcgagcaa gatgtcgaga ccgcggatgt cggggttgtc ggcgaccggt ccgtaggccg
     1921 ggccgtggat gtgcaggtag cccctgaccc catgcttggc ggctttctgg ccgaatgcgt
     1981 tgtagtcggc cgcgagctgc tcccagccgg cggtcgaggt gtcgcgcggg atctcgaagg
     2041 ccgtgccggt gtaaggcatg tccagcgcta ccgcagcctc gatcgcggcg tcgtcgaggt
     2101 tcccgtgcga gcccacgccc accaacccgt tggcctccat aagcttgcgg atctgcttcg
     2161 gtgtgatgtc gcccgtcccc tgcgtgtagc cggcgaactc gacggccgtg taacccatgc
     2221 ccgcaacggt ctcgaagacc tcggcgaagc cgacactgtc gacctgatcg cgaaccgtgt
     2281 acagcatgag gccgatcagg ttcttgggta ggtacgggtc gccggtgtag ttgctgaacg
     2341 gcgtcccctt cacatcaagt cctgaggttc cggcgaaagc gttcgacgct ccgaggatgg
     2401 tcccgccacc tgcgatcgcg cctgctccga ggaacttgcg ccggctgatc ccgcctccgt
     2461 cgttcatcga acccccattc aggagcgctt gctgcagttg taggagtact ccatgtcgtg
     2521 gagcagatcc caacccaact gggccgtgtc atagcaagtg acgacggagg aatcgctttc
     2581 gacgtggaac tcggtgatgt tctgtcgagt ctccttcgct gcaccgaaga tcttggcgaa
     2641 accctcggca ccgatctcgc cggtaccggg ttgcacaggg gctccggcgc ggaagtcctt
     2701 gacgtgcacg gtccggaagc gtcccttgta acggcgcaac tctttacaca ccgcgtcgac
     2761 ggaaccgagt ccgttgtacg cccacccgat gtccagttgc acgtgaaaga ggtccgggtc
     2821 ggtgtacttg agcatgatgt cggccgggcg aaccttggga tcgtcgtcca caggcttcca
     2881 ctcgccatcg tgacagtggt ggaaaacctg atcgaagccg gcctcccgcg cgacaggagc
     2941 agccttgctt atgtcttctg cgtatgccat ccatgccgcc ttgctgttgt cacctgaagg
     3001 cttcgatgca accccgacat aggtctgacc gacgatgttt gcgttctcga gcgtctgctt
     3061 ccacgtgtcc gggtcatatg gatggagtcc atcgccgtag tgtcccgacg ttgctgtgat
     3121 gccaacgtcc tcacatgcct ggcggaaggc ctcggggctt ccggtcgtac cccagccgag
     3181 ggcatgctcc actttctgat agccgatggc ggcgagccgc tcgagtaaga ggggtgtgtt
     3241 cgccagatac atcgaggtgc acgtaaaaag ttggataccg atctgggagt ctttgacctt
     3301 cgcggtgcaa tcgctcgccg cccacgcgac gccgggcatg gttttgagtg cagccagaga
     3361 agcaacgcct gcggcggctc cgaggaaacc tcgtcgactg atcgatcgtt gctcgacgcc
     3421 cctgaactgg ctcatctctc gctcccctac tcgtagcggc agccctgacc cagctatttc
     3481 tgcgggggcg gctttactcc tgcaccacgg cgaaactatt tgaccttacg gcttaagtag
     3541 cgcaactttt gtctagttgg tggcctaagt cactggaggg ctaggcgcga aaagggggcc
     3601 gcccgaaggc ggccccccgt ggaaaccccg ctcagtaccc gaggtcgatt acgtcgtcga
     3661 cgttctcttg cgtgacggtc gtggccggca gctggatgta gctcggaacc tccggctcga
     3721 ccaggtcgcc catcggcgcg ccgagcgcga tcagcctcgc gagacggatc gcagaggcgg
     3781 acatcgaggg gttgtacagg aacgtggcct tgtacaggtc accctcttgg atcagtttca
     3841 tcgcatcggc ggagccgcct gcgccggtga cgaacatctc gccctcacga ccggcgttct
     3901 ggatggccga cacgacaccc atcgccatgt cgtcgtcgtg cgtgtagacc gcatcgatct
     3961 ggtcctccga ctggaggatc gcttccatga cctctaggcc cttgtcgggc aggaagtcgg
     4021 cgggctgctg cgcgatgacc ttgatgccgc catcacactg cttgatcgca tcggcgaaac
     4081 ccttcgtgcg caactcggta accgagatgc ccgcgaggcc ctggatctcg acaacgttgc
     4141 ccttgcaatc gagctcctga gcgaaatagt caccggcaga cttaccgatc ccgtagttgt
     4201 cgccgccgat ccaggtgcgg tacgccgagg gcgaggagaa ctcgcggtca aggttcacga
     4261 ccgggatgcc ggctgccatc gcgtcctgtg cggcgggggt gagtgcgtct ccttcgtgag
     4321 gaaggatcac gagcacgtcg gggtcctggt tgaccagcgt ctcgacctgt gacgcctgcg
     4381 cggccgaatc gttcgttcct tcattcagga ccagttcgac gtcgtcgtac ttgtcggctt
     4441 ctgcccgtgc attctcggtg atcgcagcga gccaaccgtg gtcggcaccc ggtgcgctga
     4501 aggcgatctt gaccgtgcgt ccttcgggat tgtcatcccc ggcgtcggcc gctggttggg
     4561 ccccctcggt cgtgccgcct ccgtcatccg tacaagcgcc caagagcagc atcactgcca
     4621 aaagggcgat accaaaaatc ctcaactgct tcatctattc ctccttagat caactgtctc
     4681 tacgctgcgc aaccaccgcg atgaggacga tcaccccctt gacgatcaac tggtactcgg
     4741 tcgggatatt cagcaggttc aacaggttga agatgagagc aaacgtcatc accccaagga
     4801 acgtgccgac catcgtggcc cggccccctg cgagactcgt tcctccgatc acgaccgccg
     4861 cgatcgcatc cagctcgtaa agagcacctg cgacggggga agcgctggca agacggccgg
     4921 tgatcaagat cgccgagatg cccgcacaca acccactcaa ggcgtaaaca ctgaacacga
     4981 tggggcggac ccggatacct gcgatccggg cggcctcgcg attgccgccg atcgcgacga
     5041 cgtaacgacc gtatgccgtt cggttcagca ccacccaacc gatcgcggta agtccgatca
     5101 acacgatcac gggggcaggg atccccaaaa cctttccgtt gccgaaccag cgaacctggt
     5161 ccaacaggat cacgctgata ggtgtcttgc cactcatccg caacgcgagc cctctcccaa
     5221 tggtgagcat cgcgagcgtg gctatgaacg gaacgacgcg gccgaaggtg attgcgaaac
     5281 cgttgatcga cccgagaaga agcccgaacc cgagggcccc caggatgaat gccatcccgc
     5341 tcccgccgtc gcccaataac cccccggcga tcccggccgc agccaccagg gaaccaacgg
     5401 agaggtcgat gcccgacgaa gcgatcacga aagtcatccc gatcgccagc acgccgacga
     5461 cactcgcctg cgtgagaacc gtgaacaggt tgctcgagct caggaaggcc gacggcttcg
     5521 cgatcgagcc cgccacgatc agcacgatca gcacaccaac gagcgcgtac tcctggatgt
     5581 agatgcgacg tctggtgcga tcgtcggccg ccgtctgatc gttccgagtc actgcatcgg
     5641 tcatgaggcc acctccgcca cgggctctcg gcgcacgacg aggctgagca cctcttcttc
     5701 gctgatctct ccgcccttcg cctcgcgcac gatccgacca tccctcgtga cgaggatgcg
     5761 atcgcagaaa ccgagtagct cgggaaactc cgacgacacc atcaggatcc cgagaccctt
     5821 ttcggccagc tccgcaacga ctcggtagat ctcggtcttt gcgccgacgt caacgccgcg
     5881 agtcggttcg tccaagaaca acaccccggg agcgcgtagc agccagcggg ccaggaccac
     5941 cttctgctgg ttgccaccgg aaagctcgcg cacctgccga tccgtagaac ctggttccgt
     6001 cccgatggcg tccaactgct cgccggcgcg cttgcgttct tcgccgatgt tcaggagcag
     6061 tccccgccgg aagcgcttca gatcggcgag ggaaacgttc t
//
LOCUS       DATMED010000174         2856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1283592, whole genome shotgun sequence.
ACCESSION   DATMED010000174 DATMED010000000
VERSION     DATMED010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283592"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VNP73_04445"
     CDS             <1..304
                     /locus_tag="VNP73_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65205.1"
                     /translation="TVTLSTQDGDAVLEVADDGPGLPEGLADQIFGRFVRGAGPGDVT
                     SEAGTGLGLSIVEAVATAHGGKVEAGHANHGGALFTVTLPSTGKSAETPKKLSRVI"
     gene            complement(474..2138)
                     /locus_tag="VNP73_04450"
     CDS             complement(474..2138)
                     /locus_tag="VNP73_04450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWL65206.1"
                     /translation="MNKVKILPLGGLGEIGKNMTAIESEGRIVIVDAGVMFPTAEMLG
                     IDLVLPDFTSLRSRKDDIDAIVLTHGHEDHVGALPYVLRELGIPEAIYGGPLTLGLVR
                     SKLEERKIRDANLVILEPGERANAGPFEIETIHMSHSIPDARAVALHTKLGTILITGD
                     YRFDQTPVDGQPADIARLAELGSEGVLCLCGDSTNADRPGVGASERSVGPALREVFSR
                     CEGRIIVTCFASNVHRVQQVIDAAVELDRKVSLVGRSMRKNFNIASNLGMANAPDGIF
                     INPKEIGNYPDDRVIAISTGSQGEPLSALRRMAFNDHRDVELHSGDTIIFSATAIPGN
                     ERAVNETIDRIYEIGATVITAADAPIHVSGHGSREELKLMLNLTRPKYVFPFHGDHKR
                     IRLHAELAESVGVSPDRIFKGRNGLALEIDESSARFTDDVKAGMIFVDGVDIGDPDDV
                     ALRDRRTLSGDGVLIVVATLSGEDGSTATEPEIIFRGVPFIEGDEQVKLIDDIREAVE
                     NALADAAHDKITDAGVIQEDIHDDVAELIYKRMRRRPMILPVVVQV"
     gene            complement(2135..>2856)
                     /locus_tag="VNP73_04455"
     CDS             complement(2135..>2856)
                     /locus_tag="VNP73_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWL65207.1"
                     /translation="GEGPTLSDDETIELLEAVLDEIGSDATVICGTGSNDTRHAAALT
                     HRAAHAGAHGVLVVSPYYNKPIEDGLRAHFAEVSEAAGETPIALYNIPSRAVINMSPG
                     FMASLAKEFQNITGVKQANNGDMGPIEGLDVLAGNDDVFSRCLREGGTGGILVASHVV
                     GPRMRQIFDTASEGDHDGALDIERELADVYSAIASAPPASSIKAILSMRGLIGETQRL
                     PMVPVRGPARETLEKIAKDGS"
BASE COUNT          478 a          913 c          945 g          520 t
ORIGIN      
        1 cacggtcacg ctctcaacgc aggatggcga cgcggtgctc gaggtagccg acgacggccc
       61 cggcctcccc gagggactcg ccgaccagat cttcggccgc ttcgtccgcg gcgcggggcc
      121 cggtgacgtc acctcggagg cgggcacggg cctggggctc tcaatcgtcg aagccgtcgc
      181 gaccgcccac ggcggaaagg tggaagccgg tcacgccaac cacggcggcg ccctgttcac
      241 cgtgaccctt ccgtccacgg gcaagtccgc tgagactcct aagaaacttt caagagttat
      301 ttagggttca tttacatctc gggcttacct ttctcagtgg acgagacacc cctcgccgga
      361 gctgaccccc catttgcccg gcgagactct tggcccaagc ggccaagaaa cccctaggcc
      421 cgtcggctct ttcccctccc taagagccgg cgggccatct ttttgtcgcg gggctagacc
      481 tggacgacaa ccggcaggat catcggtctg cgccgcatgc gcttgtagat cagctcggcg
      541 acgtcatcgt ggatgtcctc ctggatgacg ccggcgtcgg tgatcttgtc gtgagcggcg
      601 tcggccagag cgttttcgac cgcctcgcgg atgtcgtcga tcagcttcac ctgctcgtcg
      661 ccttcaatga aggggacgcc gcggaagatg atctcgggct cggtcgcggt ggagccgtcc
      721 tcgcccgaga gggtcgcgac gacgatcaaa accccgtcgc cggaaagggt gcggcgatcg
      781 cgcagcgcga catcgtccgg atcgccgatg tcaacgccgt cgacgaagat cattcccgcc
      841 ttcacgtcgt ccgtgaagcg agcgcttgac tcatcgatct cgagggcgag gccgttgcgg
      901 cccttgaaga tgcgatcggg cgagacgcca accgattcgg caagctccgc gtggaggcgg
      961 atccgcttgt ggtcgccgtg gaacggaaac acgtacttcg gtcgcgtgag gttcagcatc
     1021 agcttcagct cctcgcgcga gccgtggccg gacacgtgga tcggcgcgtc ggcggcggtg
     1081 atgacggtgg cgccgatctc atagatccgg tcgatcgtct cgttcacggc gcgctcgttg
     1141 cccgggatcg cggtcgccga gaagatgatg gtgtcgccgg agtgaagctc gacgtcgcga
     1201 tgatcgttga acgccatcct ccggagcgcg gagagcggct cgccctggct gccggttgag
     1261 atcgcgatca cccggtcgtc cgggtaattc ccgatctcct tcggattgat gaagatgccg
     1321 tcgggggcgt tcgccatgcc caggttcgag gcgatgttga agttctttcg cattgagcgg
     1381 ccgacgagcg acaccttgcg gtcgagctcg acagcggcgt cgatcacctg ctgcacgcga
     1441 tggacgttgg aggcaaagca ggtgacgatg atcctccctt cgcagcgcga gaagacctcg
     1501 cgcagggccg gcccgaccga tcgctcggaa gcgccgacac cgggtctatc ggcgttggtc
     1561 gagtcgccgc aaaggcagag gacgccctcg gagccgagct cggcgaggcg cgcgatatcg
     1621 gcgggctggc cgtcgacggg cgtctggtcg aagcgatagt cgcctgtgat gaggatcgtg
     1681 ccgagctttg tgtgaagcgc gacggcccgg gcgtcgggaa tggagtgaga catgtgaatc
     1741 gtctcgattt cgaacgggcc ggcgttcgcg cgctcgccgg gctcgaggat gaccaggttg
     1801 gcgtcacgga tctttcgctc ctcgagcttc gagcgaacga ggccgagcgt cagcggtccg
     1861 ccatagatcg cctccgggat acccagctcg cgaaggacgt acggcagcgc gccgacatgg
     1921 tcctcgtggc catgagtgag gacgatcgcg tcaatgtcgt ccttgcggct tcggagcgac
     1981 gtgaagtccg gcagcacgag gtcgatcccc agcatctcgg ctgtcgggaa catcacgccc
     2041 gcatccacga tcacgatccg tccctcggac tcgatcgcgg tcatgttctt gccaatctca
     2101 ccgagcccgc cgagcgggag gatcttcacc ttgttcatga accgtccttc gcgatctttt
     2161 cgagtgtctc tcgggcaggg ccccggaccg gcaccatcgg cagacgctgc gtctcgccga
     2221 tcaaaccgcg catcgagaga atcgccttga tcgagctggc gggcggagcg ctcgcgatcg
     2281 ccgaatagac atccgcgagc tcgcgctcga tgtcgagcgc cccgtcgtga tccccctcag
     2341 aagcggtgtc gaagatctga cgcatacgcg gccctacgac gtgcgaggcg acgaggattc
     2401 cccccgttcc tccttctcgc aggcagcggg agaagacgtc atcgttaccg gccaagacat
     2461 caaggccctc gatcggtccc atgtcgccat tgttcgcttg tttgaccccg gtgatgttct
     2521 gaaactcctt ggcgagcgag gccatgaacc ccggcgacat gttgatcacc gcgcgggagg
     2581 ggatgttgta gagggcgatg ggcgtctccc ccgccgcctc ggaaacctcc gcgaagtgtg
     2641 cgcgcaatcc gtcctcgatc ggcttgttgt agtaaggcga gacgacgagg acgccatggg
     2701 cgcccgcatg cgcggcgcga tgagtcagcg cagccgcatg gcgtgtgtcg ttcgaacccg
     2761 tgccgcagat gacggttgcg tccgagccga tctcgtcgag gacggcctcg aggagctcga
     2821 tcgtctcgtc atcggagaga gtgggcccct ctccgg
//
LOCUS       DATMED010000175         5498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3206110, whole genome shotgun sequence.
ACCESSION   DATMED010000175 DATMED010000000
VERSION     DATMED010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206110"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VNP73_04460"
     CDS             <1..953
                     /locus_tag="VNP73_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HWL65208.1"
                     /translation="DFVGPKGDRRRVVGVIGALGLEPFGDASGVLPHERTMPGPKEDR
                     LALMHACPVNISPIYAIYRGAGQLRPFFDTLEQRQPEARWADDTGILHRCWVVAAPAE
                     VELIRTALAPGPLVIADGHHRYETALEFHSQNAGKPGDHGSIMTFCVDVDSEELVVLP
                     YHRALRAQTDDALSSLTEGFGAVRSDGDHEEVLNSSSFDHPFVIALKAASVVAGVSDE
                     QVVRATGERHHAWRALDVVALHEVLIPAVFGEVDELRFSRDADEIRELVDSGWDAGVL
                     LRALGATEVVDVASSGERMPQKASYFWPKALTGLVFHSLR"
     gene            complement(922..1464)
                     /locus_tag="VNP73_04465"
     CDS             complement(922..1464)
                     /locus_tag="VNP73_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65209.1"
                     /translation="MKRLRAFWQSLLGDSDRRVRLGRILGFVFVTGGFIVIAKAWDGA
                     ASINFAQGQIPYLLSGGFLGLGLIVTGSALLLLSTIRSERQVLTDIYSETSRLLSRNL
                     ARAQFSTNGSGETDGQVVATHSAYHRAECKVLQGKQGLTTVTIQQAAAEGLEPCRVCN
                     PPAPPKSEEAVSAGSGTPNP"
     gene            1630..2928
                     /locus_tag="VNP73_04470"
     CDS             1630..2928
                     /locus_tag="VNP73_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65210.1"
                     /translation="MSRRLFTHIVVVFLVSVVGSMALVSPAVGETAEPTVSARAWYWE
                     ESQTQEITLPTGEKVTAETPNPFCPAIPGSLGAPEQGCAEGRLPIEIQRGDYETPNKV
                     SAVAFDMSLVPVGSKVSKFTVTFLEAKPGCYDNADADENPNWCEQTEPINIDGKELLA
                     CRITDFLGDGDARPYKEVPKYKCANSDPTAKRKEVQTKDGETEHVWTFDLTSFASDWV
                     KTFTTNTSIMLLPKPPQGYKPGDTDDADNWRVVLVGPKAPEGKVGVQTEIVFEPGELE
                     PPVPPTTTDPGTTTTTGGSGTITGGTGTVTGGTDFGSTGTTGDLGGGGATAPTDTGGQ
                     TPPVAVAGAEQQAPGPPMYVWLAIVAGLIGWGLFRSVVLESSKGIRPNGVLSQIHALN
                     TTRRGGEVEAAAAGPSAFSSFLSGVKNSASSLVGKLTKKG"
     gene            2932..4413
                     /locus_tag="VNP73_04475"
     CDS             2932..4413
                     /locus_tag="VNP73_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL65211.1"
                     /translation="MSRINLKLVTLFGVMLLFAAACGQKPGVATGGLLPPGAEVNAQG
                     QIVDAETGEVLGTVEGGLGSTSGGSLGGSAIGGTTSGGSVSGGSTSGGTTSGGTTSGG
                     TTSGGSSGGSAPPSSGGATSTGVTSNLIKIGSHAPLTGAAPVPSGSAEKGSKLLWEWM
                     KRNKQTVNGRDVEAILKNDNYNPSQAVAVCKEMVEKDEVFLLSGLAGTDQIQACARYA
                     ASVGVPYLSAGVTENGLTGLYNYFTTSMTYNDQGPLLADMLVTKLGGKSEKNGVLMFD
                     TPNFADAHDGFMEGMQKQGGKVIYDRKVSKGAGQTEAQTVIQEMSAAGIQNVYVLTSP
                     VWYLQVLKAADTQNYKPQWLGVGISMTFDTVANVGCRGNGAIDKAKFFAPFPAWFDRN
                     KFDAEYDKAMADVYGGQGDDFVWLGWGASKQVRGLLEHAGKNLTREGFIAALEKVRNL
                     KTGIMPTLNYASNDHFGANEVHVNEARCSDNRWHTIQSFVSDF"
     gene            4510..>5498
                     /locus_tag="VNP73_04480"
     CDS             4510..>5498
                     /locus_tag="VNP73_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL65212.1"
                     /translation="MRTAVVTAAIAAILIAVIFMGLGDPTGPNAFIIIGISQGAIYGL
                     VGIGLVLVYKGARVFNFAQGEYGSMAAFIVYLFVEQWKTGLPYWVAALIAIVCVIILG
                     LIMERIVVRPLMNAPRVTLLVATIAFALTLIGIQLLLFLPEAKSFPPIVQQLPGGGNG
                     IEIFNFIIEPQRLLILAVLAALAGGLGYFFSRTDLGLAVLATSQDAFATRVVGIGVER
                     MSRFIWGSAAFLGAVAGILYVPISGALTPGVMTSTILIPSFTGAVIGGMTSLPGAFVG
                     GVVVGVIQALSNWAAGNQGVAETLFGGRLIQDILPGSPDVVLMLVLLLVLMIRP"
BASE COUNT         1007 a         1746 c         1764 g          981 t
ORIGIN      
        1 aggatttcgt cggccccaag ggcgaccggc gacgcgtcgt cggggtgatc ggagcgctgg
       61 ggctcgaacc gttcggtgac gcctccggcg ttctgccaca cgagcgcacg atgccgggcc
      121 ccaaagagga ccgccttgca ctgatgcatg cgtgtccggt caacatctcg ccgatctacg
      181 cgatctatcg gggggcgggg cagctccgtc ccttcttcga cacgctggaa cagaggcaac
      241 cggaggcccg ctgggccgac gacaccggga tactccatcg ctgttgggtg gtggcggctc
      301 ccgctgaggt cgagctgata aggaccgcgc tcgcgcccgg gcccctcgtg atcgccgacg
      361 ggcaccaccg ctacgagacc gcgctcgagt tccacagtca gaacgcaggg aagcccggcg
      421 atcacggatc gatcatgacc ttctgcgtcg acgtcgactc cgaagagctc gtggtgctgc
      481 cctatcaccg ggctctccgc gcgcagacgg acgacgcgct ctcgtctctg actgagggct
      541 tcggcgccgt gcgatcagac ggggatcacg aggaggtcct taatagctcg agcttcgatc
      601 atcccttcgt catagcgctg aaggcagcaa gtgttgttgc aggcgtgtcc gacgagcagg
      661 tcgtgcgtgc gaccggcgag cgacaccacg cgtggcgggc tctcgacgtc gtcgccctcc
      721 atgaggtcct gataccggcg gtcttcggcg aggtcgacga gcttcgcttc tcgcgcgacg
      781 ccgacgagat ccgcgagctc gttgattcag gatgggatgc gggtgtcttg ctacgcgctc
      841 tcggcgcgac cgaggtagtg gatgttgcga gctcgggtga acggatgccg cagaaggcga
      901 gttacttctg gcccaaagcc cttacgggtt tggtgttcca ctccctgcgc tgacggcctc
      961 ttcactcttc gggggggccg gcgggttgca cacgcggcac ggctccagtc cttccgcagc
     1021 cgcttgctgg atcgtgaccg tggtcaaccc ttgcttgcct tgcaggacct tgcactcggc
     1081 gcggtggtag gcgctgtgag tcgcgaccac ctgtccgtcg gtctcgcccg acccgttggt
     1141 cgagaactgg gctcgggcga gattgcgact gagaagcctg gacgtctccg agtagatgtc
     1201 ggtcagaacc tgtcgctccg agcggatcgt cgacaacagt aggagcgcgc tgccggtgac
     1261 gatcagaccg agcccgagga acccacccga gagcagataa gggatctggc cctgggcgaa
     1321 gttgatcgag gcggccccgt cccacgcctt ggcgatcacg atgaagccgc cggtcacgaa
     1381 tacgaagccc aggattcgac ccaaccggac acgccgatcg gagtccccca acagtgattg
     1441 ccagaacgcc cgcaaacgct tcatgaacca ccaagcgtag caagttcgtg acccgccggt
     1501 ggcaggtgcg accgcctagg aggtcgcgca gggaaggaaa ggggcccggt cacctagaaa
     1561 tcccagggtg ctaggctttt tcgctctagg ggtgagcgga tgcggatggg caatgtccgg
     1621 aggagatcta tgagcagaag gctctttact catatcgtcg tcgtcttttt ggtcagcgtc
     1681 gtggggtcga tggccctggt atcccctgcc gttggggaaa ctgcggaacc gacggtgtcc
     1741 gcccgtgcgt ggtattggga ggagtcccag acccaggaga tcactctccc aacgggtgag
     1801 aaagtgacgg cggaaactcc caaccccttc tgccctgcga ttcccgggtc gctcggcgcc
     1861 cccgagcagg gctgcgccga aggcagactc cccatcgaga tccagcgcgg cgattacgag
     1921 acgcccaaca aggtgtccgc cgttgccttc gacatgtcac tcgttcccgt cggctcgaag
     1981 gtgtcgaaat tcaccgtgac gttcctcgag gccaaacccg gttgttacga caacgcggac
     2041 gcagatgaga atcccaactg gtgcgagcag accgagccga tcaacatcga cggcaaggag
     2101 ctcctcgcgt gtcggatcac ggacttcctg ggcgacggcg acgctcgtcc ctacaaggaa
     2161 gtgcccaagt acaagtgcgc aaactcggac cccaccgcga agcgcaaaga ggttcagacc
     2221 aaggacggcg agaccgagca cgtctggacg ttcgatctga cgtccttcgc ctccgactgg
     2281 gtcaagacgt tcacgacgaa cacctcgatc atgcttctgc ccaagccccc tcagggttac
     2341 aagccgggcg acacggacga cgccgacaac tggcgcgtcg tgctggtcgg acccaaggcc
     2401 cctgaaggca aggttggagt gcagaccgag atcgtcttcg agcccggcga gctcgagcca
     2461 ccggttcccc ccaccacgac cgaccccggc actacgacga ccaccggcgg tagtggaacg
     2521 atcaccggcg ggaccggcac cgttaccggc gggaccgact tcggttccac cggaaccacc
     2581 ggtgacctcg ggggcggcgg cgcgacggct cctacagaca ccggcgggca aacgccaccg
     2641 gtcgcggttg cgggtgccga acagcaggcc cccggcccgc ccatgtacgt gtggctggca
     2701 atagtggccg gcttgatcgg ttgggggctg ttccgctcgg tcgtcctcga atcttcgaag
     2761 ggcatccgtc ccaacggggt tctgtcccag atccatgctc tcaacaccac gcgtcgcggc
     2821 ggtgaagtcg aagcagcggc cgccggtccg tcggccttct cctccttcct ttccggggtc
     2881 aagaattcgg caagttcgct cgtgggcaaa ctgactaaga agggataagc cgtgtcacgt
     2941 atcaacctca agctcgtaac cctgttcggc gtaatgctcc tgttcgccgc cgcctgcggc
     3001 cagaagccgg gtgtcgcgac cggcggactt ctgcccccgg gtgcggaggt gaacgcacaa
     3061 ggacagatcg tggacgccga aaccggtgag gtgctgggaa ccgtggaagg cggcctcggc
     3121 tcgacctccg gcgggagtct tgggggcagc gcgatcggcg gcaccacttc gggtggctcg
     3181 gtatccggcg gatccacgag cggggggacg acttccggtg ggactacctc cggcggcacg
     3241 acctcgggcg gatcctccgg aggcagcgct ccccccagta gtggaggcgc gacgtccacc
     3301 ggtgtcacga gcaatctgat caagatcggc tcgcacgccc cgctcactgg tgccgcaccc
     3361 gttccctcgg ggtcggccga gaagggatcc aagctgctgt gggagtggat gaagcgcaac
     3421 aagcagacgg tcaacggccg cgatgtcgaa gcgatcctga agaacgacaa ctacaacccg
     3481 agccaggccg tagcggtgtg caaggagatg gtcgaaaagg acgaggtctt cctgctgtcg
     3541 gggctggccg gaaccgacca gatccaggcc tgcgccaggt acgcggcctc cgtcggcgtc
     3601 ccgtaccttt cagccggcgt caccgagaac ggcctcacgg gcctgtacaa ctacttcacg
     3661 acgtcgatga cctacaacga tcaaggtccg ctcctggcag acatgctggt gaccaagctt
     3721 gggggcaaga gcgagaagaa cggcgttctc atgttcgaca cgccgaactt cgccgatgcc
     3781 cacgacgggt tcatggaggg catgcagaag caaggcggca aggtcatcta cgaccggaag
     3841 gtcagcaagg gcgcgggaca aaccgaggcg cagacggtca tccaggagat gagcgcagcc
     3901 gggatccaaa acgtttacgt actgacgtcg ccggtgtggt acctgcaggt cctgaaggcc
     3961 gcggacacgc agaactacaa gccgcaatgg ctgggcgtcg ggatctcgat gaccttcgac
     4021 acagtggcga acgtcggctg tcgaggtaat ggcgccatcg acaaggcgaa gttcttcgct
     4081 cccttcccgg cctggttcga ccgcaacaag ttcgacgcgg agtacgacaa agccatggcc
     4141 gacgtctatg ggggccaggg cgacgacttc gtgtggctcg gttggggtgc ctcgaagcag
     4201 gtgcggggcc tcctagagca tgcgggcaag aatctgacgc gtgaggggtt catcgccgcc
     4261 ctagagaagg tccgcaacct caagactggg atcatgccga cgctcaatta cgcctcgaac
     4321 gaccatttcg gtgcgaacga ggttcacgtc aacgaagctc gctgctccga caatcgctgg
     4381 cacacgatcc agtcgttcgt gagcgacttc tgatctcggt cgggaaaggc tccagcaaag
     4441 ggtccggcac tcccatggcg caggcaacct ggcgcggacc agggtccctg cggtaatttc
     4501 ccccctgcca tgagaacagc agtcgtaacc gcagccatcg ccgcgatcct catcgcggtg
     4561 atctttatgg ggctcggcga ccccaccggc cccaacgcgt tcatcatcat cgggatctcg
     4621 caaggcgcta tctacggcct ggtgggtatc gggctcgtct tggtctacaa gggtgcccgc
     4681 gtcttcaact tcgcccaggg tgagtacggc tcgatggcgg cattcatcgt ctacctcttc
     4741 gtcgagcagt ggaagaccgg gctcccctac tgggttgcgg cgctgatcgc gatcgtttgc
     4801 gtgatcatcc tcggactcat catggagagg atcgttgttc gaccactgat gaacgctcct
     4861 cgagtcaccc tgctcgtcgc aacgatcgct tttgcactca cgctgatcgg catccagctc
     4921 ctgttgttcc tgcccgaggc aaagagcttc cccccgatag ttcagcagct tcccggcggt
     4981 gggaacggca tcgagatctt caacttcatc atcgaacctc aacggctctt gatccttgcc
     5041 gtcctggcgg ctcttgccgg agggctcgga tacttcttct ctcgcacgga cctgggactc
     5101 gccgtacttg cgacctcgca agacgcgttc gcaacacgtg ttgtcggcat cggcgtcgag
     5161 cgcatgtcga gattcatctg ggggtcggca gcattcttgg gcgcggttgc cggcatcctg
     5221 tacgtgccta tctcgggcgc tctgacaccc ggagtcatga cgagcaccat cctcatcccc
     5281 tcgttcacgg gggccgtcat cgggggcatg accagtcttc cgggtgcctt cgtgggaggc
     5341 gtcgtggtcg gcgtcatcca ggcgttgtcg aactgggcgg cgggcaacca gggcgtcgcc
     5401 gaaacgctct tcggcggacg cctgatccag gacatcttgc cgggaagccc cgacgtggtc
     5461 ctgatgctcg ttcttctgct cgtgctgatg atccggcc
//
LOCUS       DATMED010000176         5712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1284398, whole genome shotgun sequence.
ACCESSION   DATMED010000176 DATMED010000000
VERSION     DATMED010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284398"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VNP73_04485"
     CDS             <1..696
                     /locus_tag="VNP73_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWL65213.1"
                     /translation="LRKNLLWIILITVLAVAVSFGLTFQQEDRYTAAARLLVESESDQ
                     DVDVATEAQRVVSVPVAELVIDELELTDTDAASLLSGLDVGPIEDEGAVLAIAYNSTD
                     PVQAAALANGFAESYLVYREENAIEEASEATEAITELIETTRSELDSLTGAERDTAAA
                     QLAVFQQRLADARLAETVGVATGEVLAGAGVPSEPSSPDLPRNLLFAGIAGLIVGLAI
                     AFFRARLEDPPSR"
     gene            781..2244
                     /locus_tag="VNP73_04490"
     CDS             781..2244
                     /locus_tag="VNP73_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWL65214.1"
                     /translation="MMDRRKRAVCATAVAVLGALFLPAQASALIAQPQLPPEPTVCIG
                     TIVVPGDNIQAAIDAQSEGATICLLPGTYRLRFPLFPKNGQTLVGQPGTVVSGAEVVT
                     FNQEGNLWTSTGHAQEPTPAVTRCARRARKVCNMPFRLFRDGSQMTAVASIEKVGPGR
                     FFFDLSKDTVYIGEDPTGSVFELGASPLGITGKRLEATANDVTVRGIEWQHFANQRIG
                     VISTFTGQGWLIEDNIVHHSHGCGIYGGTRSQVIDNFVHDMGQLGLCGQGEDMLVEDN
                     EIANNNLDGFDPRWEAGGAKWVNTTRLTVRNNISHDNRGPGLWTDGNNIETLYEGNTV
                     ENNTEEGIFHEISYDAIIRDNVVRNNGHELGAWGSGIQISSSPNVEIYNNTVEGPWNG
                     ISLLQEKRGKGVHGRFVLRNIYVHDNNVTMRQGTTGAHRPPAPTVALPTKLIRFEANN
                     YTLGAGKHFAWAGKALSVRQWRKLGMDKTGSVNRTQR"
     gene            complement(2235..2984)
                     /locus_tag="VNP73_04495"
     CDS             complement(2235..2984)
                     /locus_tag="VNP73_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWL65215.1"
                     /translation="MKAQPPSFDVLGVRVDAITMTDALATLDRLLRTRARGYLVFCTV
                     SSILSARDDHDVARAMSKAALVTPDGMPLVWLGKSRANTPVERVYGPDFLRAVLERGV
                     PLRHFFYGGASGVPEQVAEAVGRYEGCEVVGTYSPPTLEPGSIDHDAIPIINEARPDV
                     VWVGLGHPKQELWMSVMSPELDVPVLVGVGAAFDFLAGTKREAPGWMKRAGLQWVHRL
                     ASEPSRLWRRYIFGNARFLFLLARERLTGHR"
     gene            complement(2981..4114)
                     /locus_tag="VNP73_04500"
     CDS             complement(2981..4114)
                     /locus_tag="VNP73_04500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65216.1"
                     /translation="MAKPLTLLVLAHDLQGRGMERATIRLLREFDRDLLRPELAIASS
                     RGEFLNAVPADVPIHHLGGAGRRTSRSGGALFKLVRRLRPDCALGIHISAGRLLGAIR
                     ILKPSLSVISTEGGWPFTYIESKKGKLFVRTMISRLTYRLMSHVVVSSDMAADDLVTN
                     LGVPRRKITLIPHPCVDDEMMAQATEPVDDAPFSGQGDPIIIAVGNLHPHKDQGTLIS
                     AFAKVAKEVPSHLVLIGEGPMRGELERMVTAAHLTDRVWFMGFQRNPFKYLARSTVFV
                     SPSEAEGFDISQVEAMACGLPVVVTDAPRFMAVKDGHTGLLAPPKDPDGLAQTILRLL
                     GSSELSSTLGANAKTAALEYSSAAIARRYEELIRTVVGPNGHE"
     gene            4150..5310
                     /locus_tag="VNP73_04505"
     CDS             4150..5310
                     /locus_tag="VNP73_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL65217.1"
                     /translation="MKILFVAGEHPHFGATGGIGSYLAIIAPTLARRGHEVHVLVCRS
                     HSADDHMDGDVHLHLRPAHPVPALGRVRHLSLTHQRISAARATAQGLRDLGVFDAIEA
                     PEWMAPSLFLSNQDRARLVINLHTSIGVIAKHGDYLGRDARLADRLENRTIRRARAVT
                     SPSALLIDEMQGRGCPRDKVGIIRLPIDLAAWRPDAVLAQDEPRVLVVGRLEERKGLD
                     VLVRSMAMMPERLRQIPVVAVGRSSGQKDGLDYAGWVKGLAHDNDVRFEHHEALPRTE
                     LAKHYATARVVAVPSRFESFSMAAVEGMASGTPVVCTRTCGAAELIAGTEAGRTVPPE
                     DPAALRDALLPYLEDAPAARRAGDLARSIVQDNCDPELVASQRERVYEECAS"
     gene            5307..>5712
                     /locus_tag="VNP73_04510"
     CDS             5307..>5712
                     /locus_tag="VNP73_04510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWL65218.1"
                     /translation="MTDTGRPEVALIVPSHNRAALLEDCVMSLLEQDTQKRYEVVVID
                     DGSTDDTAERIGRLAERYPSLLRCISRPPSGLNASRNAGAAVTTADVLAFIDDDALVS
                     NSYVDAVVAGSAKHPDIDCFGGRIRLKFHGPSP"
BASE COUNT         1111 a         1768 c         1797 g         1036 t
ORIGIN      
        1 ctgagaaaga atctcctctg gataatcctg atcacggtgc ttgccgttgc cgtttccttc
       61 gggctgacgt tccaacaaga ggaccggtac accgctgcgg cccgtctcct tgtcgaatcc
      121 gagagtgatc aggatgtcga cgttgcaacg gaggcccagc gcgtggtttc ggttccagtg
      181 gccgagttgg tgatcgacga actcgaacta acagacaccg acgcggcttc gctactctcc
      241 ggtttggatg tcggccctat tgaagacgag ggtgctgtgc tcgccattgc atacaactcg
      301 acggatcccg tgcaggcagc cgcgctcgcg aacggcttcg cggagtccta cctcgtctac
      361 cgcgaggaga atgcgatcga ggaggcatcc gaagcaacgg aagcgatcac cgaactgatc
      421 gaaacgacgc gatcggaact cgactctcta accggggccg agcgcgacac ggccgccgct
      481 cagctcgcgg tattccaaca gagactcgcc gacgcgcgcc tggcggagac ggtgggcgtc
      541 gcgaccggcg aagtgctcgc cggtgcgggt gtcccgtcgg agccatcctc gcccgacctt
      601 ccgcgcaacc ttctgttcgc cgggatcgcc ggactcatcg tgggactcgc gatcgcgttc
      661 ttccgtgctc gcctggaaga ccccccgtcc aggtaacgaa tctacgcatt tcggtccgta
      721 ggttttcggc gtttcgcgcc gaaccaccct aggtagagct attccggata attaggggcc
      781 atgatggatc gtaggaagcg tgcggtctgc gcaactgcgg tggccgttct cggtgcactg
      841 tttctccctg cacaggcatc cgcgctcatc gctcagcccc agctccctcc cgaaccgacc
      901 gtgtgtatcg ggaccattgt ggttcccggc gacaacatcc aggcagcgat agatgcgcag
      961 agcgagggcg cgacgatctg tttattgccg ggcacgtacc ggctccgctt cccgttgttt
     1021 ccgaagaacg ggcagaccct cgtcggacag ccgggcaccg ttgtgagcgg agccgaggtc
     1081 gtgacgttca accaagaggg aaatctctgg acctcgaccg gtcatgcgca agaaccgacg
     1141 cccgctgtga cgcgctgtgc gcggagagct cgcaaggtct gcaacatgcc cttccgattg
     1201 ttccgcgacg gctcgcagat gacggcggtt gcttccatcg agaaagtcgg gcccgggcgg
     1261 ttcttcttcg atctcagcaa ggacaccgtt tacatcggtg aggatccgac cggcagcgtg
     1321 ttcgagctcg gtgcctcgcc tctcgggatc acggggaaga ggttggaggc tacggccaac
     1381 gacgtgacgg tgcgcggcat cgagtggcag cacttcgcca atcagaggat cggagtgatc
     1441 tcgacgttca ccggtcaggg gtggttgatc gaagacaaca tcgtgcacca cagccacggc
     1501 tgcgggatct atggcgggac aaggtcgcaa gtgatcgaca acttcgtcca cgacatgggc
     1561 cagctcggtc tgtgtgggca gggtgaagac atgctggtcg aggacaacga gatcgccaac
     1621 aacaacctcg atgggttcga cccacgctgg gaagccggcg gcgccaagtg ggtcaacaca
     1681 acgcgactga cggttaggaa caacatcagc cacgacaatc gagggcccgg gctgtggacg
     1741 gacgggaaca acatcgagac cctctacgag gggaacaccg tcgagaacaa caccgaagag
     1801 gggatctttc acgagatctc ctacgacgcg atcatccgag acaatgtcgt gaggaacaac
     1861 ggccacgagc tgggtgcgtg gggatcgggg atccagatct cgtcatcccc caacgtcgag
     1921 atctacaaca acaccgtcga ggggccgtgg aatggcatct cgctactgca agagaaacga
     1981 ggaaagggag ttcacggccg gttcgttctt cggaatatct acgttcacga caacaacgtc
     2041 accatgcggc aggggaccac gggcgcgcat cgcccaccgg cacccacggt cgcgttgcct
     2101 accaagttga tccgcttcga agccaacaac tacacgctcg gtgccggcaa acactttgcc
     2161 tgggccggca aggctctttc ggtgaggcag tggcgcaagt tggggatgga caagactggg
     2221 agtgtcaacc gcactcagcg gtgaccggtc agcctttccc gcgccaagag gaataggaat
     2281 ctggcattgc cgaagatgta acggcgccac agacggctcg gttccgacgc gaggcggtgg
     2341 acccactgga gcccggcccg cttcatccat cccggtgcct ctctcttcgt gcccgcgagg
     2401 aagtcgaatg cggccccgac tccgaccagg acgggtacgt cgagctcggg agacatcacg
     2461 ctcatccaga gttcctgttt cgggtgtccc agcccaaccc agaccacgtc gggccgcgct
     2521 tcgttgatga tggggatcgc gtcgtgatcg atcgatcccg gttcgagcgt gggtggtgag
     2581 tacgtcccga ccacctcgca cccctcgtag cgtcctaccg cctcggcaac ctgctcgggc
     2641 actccagatg ctccaccgta gaagaagtgc ctgagaggaa caccccgttc gagtactgcc
     2701 cggaggaaat ccgggccgta gacccgctcc acaggtgtgt tcgccctgct cttgccgagc
     2761 cataccaacg gcatcccatc tggtgttacc agcgcggctt tcgacatcgc gcgcgcaacg
     2821 tcatggtcat ctcgtgcact gaggatggag ctaacggtgc agaacacgag atatccgcgc
     2881 gcccgcgttc ttaggagtcg gtccaaagtc gccagtgcgt cggtcatcgt gatcgcgtcg
     2941 acgcgcactc ccagcacgtc gaaggaaggc ggttgagcct tcactcgtga ccgttgggtc
     3001 cgacgacggt acggatcaac tcttcatagc gtcgagcgat agcggcactc gaatactcca
     3061 gcgccgccgt cttggcgttg gcccccaagg tcgaagagag ttcactcgat ccgagtagac
     3121 gcaggatggt ttgcgcgagc ccgtcggggt cttttggcgg cgccagcagc cctgtgtggc
     3181 cgtccttcac cgccatgaag cggggcgcgt cggtcaccac cactggaaga ccacatgcca
     3241 tggcctcgac ctgggagata tcgaaaccct ctgcttccga cggagacacg aacacggtcg
     3301 aacgagcgag gtacttgaac gggttccttt ggaaccccat gaaccacacc cggtccgtca
     3361 ggtgcgcggc tgtgaccatc ctctcgagct cgccgcgcat agggccctct ccgatgagca
     3421 cgaggtgcga cggcacctcc tttgccacct tcgcgaacgc actgatgagc gtgccttggt
     3481 ccttgtgtgg atggaggttg cctacggcga tgatgatcgg gtcaccctgg cccgagaagg
     3541 gggcgtcatc gacgggctcg gtcgcctgag ccatcatctc gtcgtccacg cacggatgcg
     3601 ggatcagcgt gatctttcgt cgcgggaccc caagattggt gaccaggtca tcggcggcca
     3661 tgtcggagga caccaccacg tgcgacatga ggcgatatgt caaacgggag atcatcgtgc
     3721 gcacgaacag tttgcccttc ttgctttcta tataggtgaa gggccagcct ccctccgtgg
     3781 agatgacgga caaactgggt ttgaggatgc ggatcgcgcc gagcaaccga cccgccgaga
     3841 tatggatgcc cagcgcgcaa tcgggccgta gccgacgtac gagtttgaat agggcgccgc
     3901 cggacctcga cgtccgacga cccgcgccac cgaggtggtg gatcggtacg tctgccggaa
     3961 ctgcgttgag gaactccccg cgactagatg cgatcgccag ttccggtctc aacagatccc
     4021 gatcgaactc tctcagcaga cggatggtgg cacgttccat gccgcgacct tggaggtcgt
     4081 gtgcaaggac aagaagggtc agtggtttgg ccatatcggg aggtcgtgag cataggagaa
     4141 tgacgggcga tgaagatcct tttcgttgcc ggggaacatc cacacttcgg tgctaccgga
     4201 gggatcggct cctatctcgc catcatcgct ccgacgctcg cgagaagagg tcacgaggtg
     4261 catgtgcttg tgtgtcgaag ccactcggcc gatgaccaca tggacggtga tgtacatctg
     4321 cacctacgac cggcccaccc cgtaccggcg ttgggccgcg tgcgtcacct gtcactgacg
     4381 caccagagga tctcggcagc tcgcgccact gcgcaaggat tacgcgacct cggggtcttc
     4441 gacgctatcg aggcgcctga atggatggcc ccgtcactgt tcttgagcaa tcaagatcgc
     4501 gcccggttgg tgatcaacct gcatacctcg atcggggtca tagcgaagca tggggactat
     4561 ctcggcaggg acgctcgact cgccgaccgg ctcgagaaca gaaccatccg ccgagctcgt
     4621 gccgtgacct caccgtccgc ccttctgatt gacgagatgc agggacgcgg gtgcccgcga
     4681 gacaaggtag ggatcatcag attgccgatc gacctcgcgg cctggcgtcc agacgcagtc
     4741 ttggcacaag acgaaccgag ggtcctggtc gtcggccggt tggaggagcg caaggggctc
     4801 gacgtcctcg tccgctcgat ggcgatgatg ccggagcggc tgagacagat ccccgtcgtt
     4861 gcggtcggcc ggtcgtccgg acagaaggat gggctcgact atgccggttg ggtgaagggc
     4921 ctggcgcacg acaacgatgt gcggttcgaa catcatgaag cccttccccg gaccgaactc
     4981 gcgaagcact acgccacggc gcgagtagta gccgtgccga gccgtttcga aagcttctct
     5041 atggccgcgg tagaggggat ggcgtcgggc actccggtgg tctgcacgag aacctgcggc
     5101 gctgccgagc tcattgcggg caccgaggcc gggcggaccg ttccccccga agatcccgcg
     5161 gcactcagag acgcgctgct cccatacctc gaggacgcgc ccgccgcgag gcgcgcgggt
     5221 gacctggcgc gctcgatcgt gcaggacaac tgcgatcctg aactcgtggc ctcccagcgc
     5281 gaacgcgtct acgaggagtg cgcatcttga cggatacagg ccgaccggag gtcgccttga
     5341 tcgtgccgtc gcacaacaga gctgctcttc tcgaggattg cgtcatgagt ctcttggaac
     5401 aggacacaca gaagcgttac gaggtagtcg tcatcgacga cgggtctacg gacgatacgg
     5461 cggagcggat cggtcgtctc gccgagcgat acccctcgct tttgcgatgc atctcacggc
     5521 ctccgagtgg cctcaatgcc tcacgcaacg ccggagccgc ggtgaccaca gccgatgtcc
     5581 ttgcattcat cgacgacgat gcactcgtgt ccaattcgta cgtcgacgcg gtggtggcag
     5641 ggagcgccaa gcaccccgac atcgactgct tcggggggag gatccggctg aagttccacg
     5701 gtccttcacc tc
//
LOCUS       DATMED010000177         9725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3584007, whole genome shotgun sequence.
ACCESSION   DATMED010000177 DATMED010000000
VERSION     DATMED010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3584007"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..464)
                     /locus_tag="VNP73_04515"
     CDS             complement(27..464)
                     /locus_tag="VNP73_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWL65219.1"
                     /translation="MDSKDRKILELLQEDGRLGYAAIGKRVKLSASSVHDRVRKLEKA
                     GVIRGYRADVDLPAAGFPITAIVSLALSPSSPADIPGLIAEFPLVESCYSVAGDNSYA
                     LMVRAPSTAALEDLLDGLRATLEVTTRATIVLSTPFEHRPLEL"
     gene            562..1935
                     /locus_tag="VNP73_04520"
     CDS             562..1935
                     /locus_tag="VNP73_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HWL65220.1"
                     /translation="MLRHRAAFAARADTPQPYRYESARDFSEPDWRRFPGYRDVTETE
                     WNNAKWQRLHTVKDLAGLQRVFGDLLPADLVESIKRDQAERATMSMLVPPQMLNTMNE
                     RDLWRDPIRHYMVPAFADRDPEWPSHPYSTRDSLHEADMWAVEGLTHRYPTKVLAELL
                     ATCPQYCGHCTRMDLVGNNVPQVIKHRFELRQQDRYRAILEYLVDTPTVRDVVVSGGD
                     IANLPIAHVEEFVGALIEIPHIKDIRLATKGLMGIPQHFLQDEVAAGMERLATKALAH
                     DVDLAVHTHINHAQSVTPLVAKATKRLLEMGFRDVRNQGVLLRGVNATTEDLLDLCFR
                     LLDHAKVMPYYFYMCDMIPNAEHWRVSLAQAQELQHAIMGYLPGFATPRVVCDVPFVG
                     KRWVHQADSYDRELGISYWSKNYRTSLEPDDATTYKRNYRYYDPIDTLKEGGRDHWRE
                     VVAATTA"
     gene            1949..2953
                     /locus_tag="VNP73_04525"
     CDS             1949..2953
                     /locus_tag="VNP73_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HWL65221.1"
                     /translation="MERFGIHRSLEPPGVLPHQADRLNTSLPLKDDEVLIDVDLINVD
                     SASWQQIRESTDGDVKAIVARILEIVREHGKLQNPVTGSGGMLVGTVVALGTHRVEPA
                     IGTRIASLVSLAVTPLVLDEIVALDSGSERVKVKGTAILFGSSPYVEVPDDLPDHTVL
                     SVLDVCGAPAWMARLAKPGMNVVVLGAGGKSGLLSVAQALRSVGPSGRVLGLCYPPPT
                     TSVVEHLGAEAAAVDCTDPGAVIECVTEIFEGATADLVFVCANVPGCEGAAVLACSDE
                     GRIVFFSMATSFVAAALTAEGMGKSCEMTIGNGYVPGHAELALNLVRDEPALAARLAG
                     "
     gene            2953..4485
                     /locus_tag="VNP73_04530"
     CDS             2953..4485
                     /locus_tag="VNP73_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWL65222.1"
                     /translation="MRKLFHSGRIRRLPGGAFADWLVVEDQRVVAVGTGDPPAADESV
                     DLDGAWLLPAFADGHVHLPVTGLEEIGLDFRGERSVDAILNAFSQRADSSGAVLYGGN
                     FEDPLDRPISRLDLDGAVGDRPALLSRVDKHSCIVSTALLDKLEVEGLEGVDRDADGH
                     PTGYLREKACSQAWSWLDANLTDAEQTEAVRAAARKAYSKGVASVHEMFVVEWRGWSA
                     LGGFLRAIAEVDLKVVTYCGTDEVSRVKKMGFPRIGGDYFLDGAFGSHTAWLSEPYAT
                     RPPLGTPESGISYRNDEDLLEFFMDAHHQGLQVAVHAIGDAAIEQAITTWEAVADKVG
                     VYAVRDRGHRLEHFEFSTDDHIARAANLGLRLSVQPVFDLYWGGDAKLYSQRMGWERA
                     AQMNRFGSMLAAGLLLGSGSDSTVTPMDPFMQLVALREHHVPKERMGDIESLRLVTMG
                     ARALAEGPTLAGTVEPAAPADLVLLDRDPATTHGDELLATEVLGTWIDGRRVWPEGEA
                     DE"
     gene            4482..6065
                     /locus_tag="VNP73_04535"
     CDS             4482..6065
                     /locus_tag="VNP73_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HWL65223.1"
                     /translation="MTFERPTGRLQIDHEAVAECRELAAEIAAPIESLAATHTTVSIE
                     RACLRLVGVDGVVGDGDSAVPVPNLVVDRIRDATGLEGGALVPFFHMVESGCGDIQSE
                     ANRIATGERTVTAPEDLDHAIAVERAQREAHEAVARIANATEERAAKIEAVGESDKPW
                     FYEIVATGNIHEDIPQAQAAAREGCAVIAVIRSTGQSLLDYVPYGATTDGFAGTFATQ
                     ENFRLMRTALDEVSENLGRYVRLTNYASGLCMPEIAAMAALERLDMMLNDCMYGIIFR
                     DINMQRTFVDQHFSRMVHALAGIVINTGEDNYLTTADAFESAHTVLASQFLNERLAHA
                     AGVPEEAMGLGHAFEIDPDVPNQILYELAHAQLIRECFPNAPLKYMPPTKHMTGNVFQ
                     GFLYDGMFNLVGALTGQDIILLGMMTEGVHTPFLSDRDLALENARYIFGGAHALAGEL
                     RFEPGGLVERRAAQVLEECRQMLRKVADIGMFAAIEAGMFADTVRTRTGGRGLDGVVP
                     RGDGYTNPVAELLAQKVGV"
     gene            6065..6802
                     /locus_tag="VNP73_04540"
     CDS             6065..6802
                     /locus_tag="VNP73_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HWL65224.1"
                     /translation="MAVIRPYGDETDDGMVQVSFTLPVEAGERAREAALAFAGQMGLT
                     RAQVVHMKDIGERFTFFIVYGASQHSVDLDSIVVTERDFPELSYQEVNRLIRTGLKRR
                     LVVVGACTGTDAHTVGLDAILSMKGFAGDHGLEYFPEFEVVNMGSQVTPEEVVKAASE
                     RGADAVLISQVVTQRDAHIHHLAQVRSALEGAGLRDRLLLVGGGPRFSTDQAGELGYD
                     RIFGRGTKPSEVAAYLAWAVTQRRAAA"
     gene            6799..7209
                     /locus_tag="VNP73_04545"
     CDS             6799..7209
                     /locus_tag="VNP73_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HWL65225.1"
                     /translation="MTERISASLRIRLSSADAHYGGDLVAGGRLLELIGDVATELCIR
                     SDGDEGLLAGYHSVEFLAPVRAGDFVEIRGVIVAAGQTSRKIECEVLRYASPRPDVSD
                     SAADLVEHPEVVARATGTCVVKQDRQRNTEGSGA"
     gene            7206..7922
                     /locus_tag="VNP73_04550"
     CDS             7206..7922
                     /locus_tag="VNP73_04550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWL65226.1"
                     /translation="MKRVDGIRAVTYDCWGTLLKDRDWEGAMAKRQSALLRFLDLAEE
                     EAQALLAEAWARHDEAWKQVETFGAGRMAAYCLEARGIFDDEPISELTREFELASTET
                     GVDAVDGARETLAALRESNIRLGLVCDTGFTPGSVVKTLLDDNGLLEFLEVLCFSDEV
                     GVPKPGAEIFNKALAELGVHPPEAVHIGDLKRTDIAGAHDFGMKAIRFRGVHDDQSDA
                     PEARVVIDRHEELLELLNLK"
     gene            7919..8338
                     /locus_tag="VNP73_04555"
     CDS             7919..8338
                     /locus_tag="VNP73_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65227.1"
                     /translation="MSARATWSRAPWSIQLYAMLIIATWLAGLIITPDDFVPVVFRLY
                     EAGTGVLIFVLLLHGLFTRIRFVWLVAIGWGIWGLLGPLLAILFAGASIEFFLSGMNL
                     VYTVGAVIQVAILFAPATRRWIERPMPPPGGEAAATP"
     gene            complement(8331..8531)
                     /locus_tag="VNP73_04560"
                     /pseudo
     CDS             complement(8331..8531)
                     /locus_tag="VNP73_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
     gene            complement(8542..>9725)
                     /locus_tag="VNP73_04565"
     CDS             complement(8542..>9725)
                     /locus_tag="VNP73_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65228.1"
                     /translation="RDPSASANRMHGKLFVTTEDGDEHYCSGTAIQSDNQSVVFTAAH
                     CLYQEEFDGWYESAMFVPAYLSGDAPFGKWVADEGFVPNDWFRYETPRVDLGALVMKS
                     NEDTTLVDAVGGMRARFGQSRDQEFEAFGYPGAEPFDGQRLYVCEAPRYYDDPRAPGD
                     GPDPLGIGCDMTPGASGGAWVIDGRYVNSVTSYSFDGLANVLLGPYFGDTAQELFGAA
                     ESEVVDEDGPEQVEPTPRPAASASPERSARVSDPDDSPGMLDISEVSLVTESGTLTVR
                     LRTHDAWSKKVLGRSTNFFRIDLDISGGPRPDVYLVLDRVDDRPVATAYAYQRGPDRV
                     VAGGLLSRPQRTEVIAKFSEGVLAGSTHVRWYLLSQFRGKERCQQTCWDFAPEGTPAA
                     L"
BASE COUNT         1758 a         3083 c         3151 g         1733 t
ORIGIN      
        1 tttcatgaga aaacgtgcag gagtcgctag agctccaggg gccggtgctc gaacggggtc
       61 gacaacacga tcgtcgccct cgtggtgacc tccagcgtgg ctctcagacc gtccaggagg
      121 tcctccaggg ctgccgtcga gggcgcccgg accatcaagg catacgagtt gtcgccggcc
      181 accgagtagc aggactcgac cagtgggaac tcggcgatca gccccgggat gtcggcaggg
      241 gaggacgggc tcagagcgag gctcacgatc gccgtgatcg ggaatcccgc agccggcagg
      301 tccacatcgg cccggtagcc ccggatcaca cctgccttct ctagcttcct gactcggtcg
      361 tggacggagg aggccgacag cttgacccgc ttacctatgg cggcatagcc gagacggccg
      421 tcttcctgta gcagttcgag gatcttccgg tctttggagt ccatgtccga acatcattcc
      481 ctagatagct aatatcgcct aatattatcc ggagaagtcg tcgataattc aaggaccgcc
      541 gtggacccca cccgcaacag cttgctgcgt caccgagccg cattcgcggc ccgtgccgat
      601 acgccccagc cctaccgcta cgagtcggca cgcgacttct ccgagcccga ctggcgccgg
      661 ttccccggct accgcgatgt caccgagacc gagtggaaca acgccaaatg gcaacggctc
      721 cacaccgtga aggacctcgc cggcctgcag cgcgtcttcg gcgatctcct acccgccgac
      781 ctcgtggagt cgatcaagag ggaccaggcc gaacgcgcca cgatgtcgat gctcgttccg
      841 ccccagatgc tcaacacgat gaatgagcgc gatctatgga gagacccgat ccggcattac
      901 atggtcccgg ccttcgcgga ccgcgacccc gaatggccga gccacccgta ctcgacgcgc
      961 gattcgttgc acgaggccga catgtgggcg gtcgaaggcc tcacccatcg gtatccgacg
     1021 aaggttctgg ccgagctcct cgccacgtgt ccccagtact gcggacattg cacccggatg
     1081 gatctggtcg ggaacaacgt cccgcaggtc atcaagcatc gcttcgagtt gcggcagcaa
     1141 gatcgctacc gggccatcct tgaataccta gtcgacacac cgacggtgcg cgacgtcgtc
     1201 gtttcgggag gagacatcgc caacctaccc atcgctcatg tcgaagagtt cgttggagcg
     1261 ttgatcgaga tacctcacat caaagacata aggctggcga ccaaggggct gatggggatc
     1321 ccacagcact tcctccaaga cgaggttgcg gccggcatgg agcgcctcgc aacgaaggcc
     1381 ctcgcccacg acgtggatct ggccgttcat acgcacataa atcacgccca gtccgtgacg
     1441 cctttggtgg ccaaggcaac gaaaaggctt ctcgagatgg gcttccgcga tgttcgcaac
     1501 cagggcgtcc ttcttcgcgg cgtgaacgcg accaccgaag acctgctcga tctctgcttc
     1561 cggttgctcg atcacgcgaa ggtgatgccc tattacttct atatgtgcga catgatcccg
     1621 aacgccgagc attggcgtgt ttcgctcgct caagcgcaag agttgcaaca cgcgatcatg
     1681 ggttatctgc ccgggttcgc tacgccgcgc gtcgtttgtg acgttccctt tgttggaaaa
     1741 cgctgggtgc atcaagccga ctcatacgat cgcgaactag gcatctctta ctggtccaag
     1801 aactatcgga cgagtctcga gcccgacgac gcgactacgt ataagaggaa ctaccggtat
     1861 tacgacccga tagatacgct taaggagggg ggccgcgatc attggcgtga agtggtcgcg
     1921 gccaccactg cttgacgagg agccgatcgt ggaacgcttc ggcatccacc ggtcgcttga
     1981 gccaccgggg gtgctgccgc accaagccga ccgtctcaat acctcgcttc cgctgaagga
     2041 cgacgaggtg ttgatcgacg tcgacctgat caatgtcgac tctgcctcat ggcagcagat
     2101 ccgcgagtcg accgacggcg acgtcaaggc gatcgttgcg cggatactcg agatcgtccg
     2161 cgaacacggg aagctccaga acccggtgac gggatccggt gggatgctcg tcggaacggt
     2221 cgtagcgctt ggtacacatc gcgtcgaacc cgcgatcgga acgaggatcg cgtcactcgt
     2281 ttctcttgcg gtcacacctc tcgtgctgga tgagatcgtg gcactggatt cgggctccga
     2341 gagggtcaaa gtcaagggaa ccgcgatcct gttcggatcg agtccctatg tcgaggtacc
     2401 cgacgatctc cccgaccaca ccgttctctc tgtcctcgac gtgtgcggcg cgccggcgtg
     2461 gatggcgcga ctggccaagc ctgggatgaa cgtcgtcgtt ctcggggccg gcgggaaatc
     2521 gggtctgctc tcggtcgcgc aggcgctgcg gagcgtcgga ccttcgggcc gcgtcctggg
     2581 tctctgctat ccaccgccga caacctcggt ggtcgaacat ctgggggccg aagccgccgc
     2641 ggtcgactgc accgaccccg gggcggtgat cgagtgcgtg accgagatct tcgagggagc
     2701 tacggccgac ctcgtgttcg tatgcgcgaa cgtccccggc tgtgaaggag ccgccgtgct
     2761 ggcctgctcg gacgaaggca ggatcgtgtt cttctcgatg gcgacctctt tcgtcgccgc
     2821 cgccttgacc gcggaaggta tgggaaagtc ttgtgagatg actatcggca acggttacgt
     2881 cccggggcat gcagaactcg ccctcaacct cgtccgcgac gagccggcac tggccgcccg
     2941 actcgccggt tgatgcgaaa gcttttccac tcaggtcgca tccggcggct cccaggggga
     3001 gcattcgccg actggctcgt ggtcgaggat cagagggtcg tcgccgttgg cacgggtgac
     3061 ccgccggccg cggatgaatc ggtcgacctc gatggggcat ggctactgcc tgcattcgcc
     3121 gatggacacg tgcaccttcc cgtcaccggg ctcgaagaga tcggcttgga cttccgtggc
     3181 gagcggtctg tagacgcgat cctgaatgcg ttctcgcaac gcgcggactc ctccggtgcg
     3241 gttctatacg gagggaactt cgaggaccca ctggaccggc cgatctcgcg ccttgacctg
     3301 gatggggccg tgggggaccg gcccgcactc ttgtcgcgcg tcgacaaaca ctcgtgcatc
     3361 gtctccacgg ccttgctcga caagctcgaa gtggagggtc tcgagggcgt ggaccgcgac
     3421 gccgacggac atccgacggg ttacctacgc gagaaggcct gttcccaggc ctggtcgtgg
     3481 ctcgacgcga acctgaccga cgccgagcaa acggaagccg ttcgggcggc cgccaggaag
     3541 gcttactcga agggcgttgc gagcgtgcac gagatgttcg tcgtcgagtg gcgcggatgg
     3601 tcggccctgg gcggtttcct ccgcgctata gccgaggtcg acctcaaggt cgtcacctat
     3661 tgcgggacgg acgaggtgag tcgggtgaag aagatgggct tcccgcgcat cggaggggac
     3721 tacttcctcg acggggcgtt cgggtcgcac accgcgtggt tgtcggagcc gtacgcgacg
     3781 cggcccccac tcggaacgcc cgagagcggc atctcgtacc gaaacgacga ggacctgctc
     3841 gaattcttca tggatgccca ccaccagggc ttgcaggttg cggtgcacgc gatcggcgac
     3901 gcggcgatcg agcaggcaat caccacctgg gaggccgttg ccgacaaggt cggcgtatat
     3961 gcggtgcgcg accggggaca ccggctcgag cacttcgagt tctccaccga cgaccacatc
     4021 gcgcgcgccg ccaatctggg tctgcgcctc agcgttcaac cggtgttcga cctctactgg
     4081 ggaggcgacg caaagctcta ctcgcagcgg atggggtggg agcgggccgc acagatgaac
     4141 cggttcgggt cgatgctggc cgcgggtctg ctactcgggt cggggtcgga ttcgacggtc
     4201 acgccgatgg atcccttcat gcaactcgtc gccctgcgcg agcatcatgt tcccaaggaa
     4261 cggatgggcg acatcgagtc gctccgcctc gtgacgatgg gggcacgggc gctcgcagaa
     4321 ggacccacgc tggccggaac cgtcgagccg gcggctcccg ccgatctcgt gcttctcgat
     4381 cgagatcccg ctaccacgca cggagatgag ctcctcgcga ccgaggttct cggcacctgg
     4441 atcgacggca ggcgcgtgtg gcctgagggc gaggccgacg agtgaccttc gaacgcccta
     4501 ccggccggct ccagatcgat cacgaggccg tcgccgagtg ccgcgagctt gccgccgaaa
     4561 tagcggctcc catcgaaagc ctggcggcca cgcacacgac ggtttcgatc gaacgggctt
     4621 gtcttcggct cgtgggagtc gatggggtcg tgggcgatgg agattccgcc gtcccggtgc
     4681 cgaacctggt cgtcgatcgc atccgtgacg cgaccggcct ggagggcgga gcgctcgtcc
     4741 cgttcttcca catggtcgag tccggttgcg gcgacatcca gtccgaagcc aaccgcatcg
     4801 cgacggggga gcggacggtt acggcccccg aagatctcga tcacgccatc gcagtcgagc
     4861 gcgcgcagag agaggcccac gaggcggtcg ctcggatcgc gaacgcaacc gaagagcgcg
     4921 cggccaagat cgaagccgtt ggtgaatccg ataagccgtg gttctacgag atcgtcgcta
     4981 ccgggaacat ccacgaagac atcccccaag cacaggcggc cgcgcgtgag ggatgcgcgg
     5041 tgatcgccgt gatccgctcg acggggcaat cgctgctcga ttacgtcccg tacggcgcga
     5101 ccacggatgg tttcgccggg acgttcgcaa ctcaagagaa cttccgtctg atgcggacgg
     5161 ccctcgacga agtgtccgag aacctcggtc gttacgttcg cctgacgaac tatgccagcg
     5221 gcctgtgtat gcccgagatc gctgcgatgg cggccttgga acggctggac atgatgctca
     5281 acgactgcat gtacgggatc atcttccgcg acatcaacat gcagagaacg ttcgtggatc
     5341 aacacttctc gcgcatggtg cacgccctgg ccggcatcgt catcaacacc ggcgaggaca
     5401 actacctgac gaccgcggac gccttcgagt cggcgcacac cgtgctggcc tcccaattct
     5461 tgaacgaacg cctggctcac gcagccgggg tgccggaaga ggcgatgggt ctcgggcatg
     5521 cgttcgagat cgatcccgat gtgcccaacc agatcctcta cgagctcgcc catgcgcagt
     5581 tgatccggga gtgtttcccg aacgcgccgc tgaagtacat gcccccgacc aagcacatga
     5641 cgggcaacgt cttccagggc ttcctctacg acgggatgtt caacctggtc ggcgcgctga
     5701 cgggccagga catcatcttg cttgggatga tgacggaggg ggtccacaca ccatttcttt
     5761 ccgaccgcga ccttgccttg gagaatgctc gctacatctt cggaggtgca cacgcgctcg
     5821 ccggggaact cagattcgaa cccggcggtt tggtcgaaag gcgcgccgct caggtgctcg
     5881 aggaatgtcg gcagatgctg cgaaaggtcg ccgacatcgg catgttcgcg gcgatcgagg
     5941 ccggcatgtt cgccgacacc gtgaggactc gcaccggtgg gagggggctc gatggagtcg
     6001 tcccacgggg cgacggttac accaaccctg tagcggagct gctcgcccag aaagtcgggg
     6061 tgtgatggcc gtcatcagac cctacggaga cgagaccgac gacggcatgg tccaggtctc
     6121 tttcacgctc ccggtcgaag ccggcgaacg ggcacgcgag gccgcactcg ccttcgccgg
     6181 gcagatgggg ttgacccggg cgcaggtcgt tcacatgaag gacatcggag agcgcttcac
     6241 cttcttcatc gtctacggtg catcgcaaca ctccgtggac ctcgattcga tcgtggtgac
     6301 cgaacgagac ttccccgagc tgtcgtacca ggaggtcaat cgcctgatcc ggaccggttt
     6361 gaagcgcagg ctggtggtgg tcggggcgtg caccggcacc gacgcgcaca ctgtaggcct
     6421 ggacgcgatc ctctcgatga agggattcgc cggcgaccac gggctcgagt acttcccgga
     6481 gttcgaggtc gtgaacatgg gctcccaggt gacgcccgaa gaggtcgtta aggccgcatc
     6541 cgagcgcggt gccgatgccg tactcatctc tcaggtcgtg acccaacggg acgcccacat
     6601 ccatcacctc gcgcaggtcc gctctgccct cgaaggcgcg ggtttgcggg atcggctgtt
     6661 gctggtggga ggcggtccgc gcttctcgac cgaccaggcc ggcgagctcg gctacgaccg
     6721 gatcttcggc cgcggcacga aaccgtccga ggtggctgcc tatctagcct gggcagtgac
     6781 acagaggagg gccgccgcgt gaccgaacgc attagtgctt ccctccgcat acgactttcc
     6841 tcggccgacg ctcattacgg gggcgatctc gtggccggag gaaggctgct cgaactgatc
     6901 ggtgacgtcg cgacggaact ctgtatccgt tccgatgggg acgaaggact actcgcgggc
     6961 taccactcgg tcgaattcct ggcccccgtg cgcgccggag acttcgtcga gatccgtggc
     7021 gtcatcgtcg ccgcaggtca gaccagccgc aagatcgagt gcgaggtact cagatacgct
     7081 agccccaggc cggacgtctc ggattcggcg gccgacttgg tggaacatcc cgaagtggtc
     7141 gcgcgagcga ccggaacgtg cgtagtcaag caagaccggc aacgcaacac cgaggggagt
     7201 ggtgcatgaa gagggtggat ggcatccgag cagtgacata tgactgctgg ggcactttgc
     7261 tcaaggaccg cgactgggag ggcgcgatgg ccaaacgcca aagcgccctc cttcgttttc
     7321 tggacctggc cgaggaagag gctcaggcac tgctggcgga agcatgggcc cggcacgacg
     7381 aagcgtggaa gcaggtagag actttcggcg cagggcggat ggcggcgtac tgtttggaag
     7441 cacgcgggat cttcgacgac gagccgatct ccgagctcac ccgcgagttc gaattggcca
     7501 gcaccgagac gggagtcgac gcggtggatg gtgctcgcga gaccttggcg gcgctccgcg
     7561 aatcgaacat cagactcggc ctcgtgtgcg acacgggttt cacacccggc agcgtcgtga
     7621 agacgttgct ggacgacaac ggtctactcg agttcctcga agttctttgc ttctccgacg
     7681 aggtcggcgt tccaaagccg ggggccgaga tattcaacaa ggctctggcc gagctcggcg
     7741 ttcacccgcc cgaggcggtg cacataggcg acctgaagcg gaccgacatc gcgggagccc
     7801 acgacttcgg catgaaggcc attcgtttca gaggcgttca cgacgatcag tcggacgcgc
     7861 ctgaggctcg cgtcgtgatc gaccggcacg aggagctact ggagttacta aacctcaagt
     7921 gagcgcgagg gcgacctggt cgcgcgcgcc gtggtcgatc cagctctacg cgatgctcat
     7981 aatcgcgacc tggctggccg gactgatcat cactccggat gatttcgtcc cggtggtgtt
     8041 tcgactgtac gaagcaggga cgggcgtcct catctttgtc ctactgctgc atggactatt
     8101 caccaggatc cgattcgtct ggctagttgc gatcggctgg gggatctggg gtctactcgg
     8161 cccgctactt gccatcttgt tcgcgggagc ctccatcgaa ttcttcctgt ccgggatgaa
     8221 tctcgtgtac accgtcggcg ccgtgatcca ggtcgcgatc ctgttcgcgc ccgccacgag
     8281 gcgctggatc gagagaccaa tgcctccgcc cggcggtgaa gcggcagcta cgccttaacg
     8341 accagatcgc cgagcagctc gcgggtcgcg gcggcgacct gctcgacggc gcggtcgaag
     8401 acctcctggt tcatgcggga gggcttgcgg taaccgctga tcttgcggac gtactggaga
     8461 gcggctgcct ccatgtcctc gacggtggcg ggaggctcgg cgttcctgag agtcttgatg
     8521 cttctacaca tgccgggaag gctatagggc ggcgggcgtg ccttcgggag cgaaatccca
     8581 acatgtctgt tggcaccgct ccttgcctcg gaactgactg agtaagtacc aacgcacgtg
     8641 ggtggaaccc gccaggacac cctccgagaa cttcgcgatc acctcggtcc gctgtgggcg
     8701 cgacagcaag ccgccggcca ccacccgatc ggggccccgc tggtacgcgt aggccgtggc
     8761 caccggccgg tcgtcgaccc ggtccagcac cagatagacg tccggtcgcg gcccccccga
     8821 gatgtcgaga tcgatcctga agaagttggt ggagcgcccc agcactttct tgctccacgc
     8881 gtcgtgggtc ctgagacgca cggtcagagt gcccgactcc gtaacaagcg acacctctga
     8941 gatatcgagc atgcccggcg agtcatccgg gtcgctcacc cgcgccgagc gttccggtga
     9001 ggccgacgcg gccggtcggg gagtgggctc cacctgctcg ggaccatcct cgtcgaccac
     9061 ctccgactcc gccgcaccga acagctcctg cgcggtatca ccgaagtagg ggccgagcag
     9121 cacgttcgcg agcccgtcga agctgtaact cgtgaccgag ttcacgtagc gtccatcgat
     9181 cacccacgcg cctccgcttg cgcccggggt catgtcgcaa ccgatgccca gcgggtcggg
     9241 tccgtcgccg ggtgctctgg ggtcgtcgta gtagcgcgga gcttcacaga cgtagaggcg
     9301 ctgtccgtcg aagggttcgg cgcccggata gccgaaggct tcgaactcct ggtcgcggct
     9361 ctgaccgaat cgggcccgca tcccgccgac cgcgtccacc agcgtggtgt cttcgttcga
     9421 tttcatgacg agcgcgccca ggtccacgcg cggcgtctcg tagcggaacc agtcgttggg
     9481 gacgaagccc tcgtcggcca cccacttccc gaacggggcg tcaccggaca ggtaagcggg
     9541 gacgaacatg gcgctctcgt accaaccgtc gaactcctcc tggtagaggc agtgggcagc
     9601 ggtgaacacg acactctggt tgtcgctctg gatagcggtg cccgagcaat agtgctcgtc
     9661 gccatcctcg gtcgtaacga acagcttgcc gtgcatccga ttggccgacg cgctgggatc
     9721 gcgga
//
LOCUS       DATMED010000178         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3965302, whole genome shotgun sequence.
ACCESSION   DATMED010000178 DATMED010000000
VERSION     DATMED010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3965302"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VNP73_04570"
     CDS             complement(<1..925)
                     /locus_tag="VNP73_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWL65229.1"
                     /translation="MNILVLGGDGYLGWPTAMHMARLGHDVSVVDNFLRRGMSTELGA
                     GSLTPIRSLQERIKAFNDVTGCSIRSFVGDLTDPSFVDEVFRATEPDAVVHYGEQPSA
                     PYSMIDRRHAVATQRNNVEGTLNVLYAIRDLAPDAHLVKLGTMGEYGTPNIDIEEGFI
                     DISHNGRNDTLPFPKMPGSMYHLTKVHDSHNIHFACRIWGLRATDLNQGVVYGIRTDE
                     TDLDERLCTRFDYDEVLGTALNRFCVQAVVGYPLTVYGKGGQTRGFLNIVDTIQCVRL
                     ALENPAAGGEYRVFNQFTEQFSVLDLAEKVRS"
     gene            1084..>2430
                     /locus_tag="VNP73_04575"
     CDS             1084..>2430
                     /locus_tag="VNP73_04575"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWL65230.1"
                     /translation="MADQEVEKTIEALLEERRTFEPSESFVEQAHANDPALYAEALRD
                     PDHFWSQQAERLDWATRWDTVLDWDPPHHKWFVGGTLNVAHNCLDRHLKSNGDRVAYH
                     WIGEPGETRAVTYRELHEEVCRLANALKELGISKGDRVAIYLPMIPELPAAMLACARI
                     GAPHSVVFGGFSAESLRDRINDAEAKVLITADGGYRRGEIVPLKENADHALAGTPSIE
                     TVIVVRRTEHDVPWQQGRDVDYAQLVASAPVDCPAEPMDAEDMLYILYTSGTTGKPKG
                     IVHTTGGYLTGVAATHRLIFDIKPESDVYWCAADIGWVTGHSYIVYGPLVNGCTSILY
                     EGAPDHPDKDRWWSIVEEYNVTILYTAPTAIRTFMKWGPDFPAKHDLSSLRLLGSVGE
                     PINPEAWIWYQEFIGSGRTPIVDTWWQTETGAICITPLPGITTLKPGSATRPFPGVF"
BASE COUNT          483 a          777 c          741 g          429 t
ORIGIN      
        1 cggagcgcac cttctcggcc agatcgagaa ccgagaactg ctcggtgaac tgattgaaca
       61 ctcggtactc gccacccgct gccggattct cgagcgcaag ccgcacgcac tggatcgtgt
      121 cgacgatgtt caggaagccg cgcgtctggc cgcccttccc gtagaccgtc aggggataac
      181 ccacgacggc ttgaacgcag aaacgattga gagcagtgcc gagaacctcg tcgtaatcga
      241 acctggtgca gagacgctcg tcgagatcag tctcgtccgt cctgatcccg taaacgacac
      301 cctggttgag atccgtcgcg cgcagccccc agatgcgaca cgcgaagtgg atgttgtgcg
      361 agtcgtgaac cttcgtcagg tgatacatcg accccggcat cttggggaac ggcaacgtgt
      421 cgttacggcc gttgtgcgag atgtcgatga acccctcttc gatatcgata ttcggggtcc
      481 cgtactcccc catcgttccc agcttgacga ggtgagcgtc gggagcgaga tcgcggatcg
      541 cgtatagaac gttgagcgtg ccttcgacat tgttgcgttg ggtcgccacg gcatgcctgc
      601 gatcgatcat cgaatagggc gcgctcggct gctcgccgta atgcacaact gcatcgggct
      661 ctgtggcccg gaaaacctcg tcgacgaagg atgggtcggt gagatccccg acgaaggacc
      721 ggatggagca accggtcacg tcgttgaatg ccttgatccg ctcctgaagc gaccggatag
      781 gcgtgagact gccggccccc aattcggtcg acatccctct acgaaggaag ttgtcgacga
      841 ccgagacgtc atgaccgaga cgagccatgt gcatcgccgt cggccaacct aggtatccat
      901 caccgcccag tacgaggatg ttcacggcgg cacaatactt tttcccgtcg gcggccgagg
      961 ccgtcggcgt tcgacggact gattcacttg caggcgacgg cgcagccatc gcctttcgga
     1021 tcaccttttt tccgccggcg gccgaggccg tcggcgttcg acggactgac ctactatcgg
     1081 gggatggccg accaggaggt cgaaaagacc atcgaggcgc tcctcgagga gcgccggacg
     1141 ttcgagccgt ctgaatcgtt cgttgaacag gcacacgcca acgaccccgc gctctacgcc
     1201 gaagctctgc gggatcccga ccacttctgg tcgcagcaag cagagagact cgactgggcc
     1261 acgcggtggg acacggtcct cgactgggac ccgccccacc acaagtggtt cgtagggggc
     1321 accctcaacg tcgcccacaa ctgtctcgat aggcatctca agtcgaacgg ggaccgggtc
     1381 gcgtaccact ggatcggcga acccggcgag acgcgcgcgg tcacctatcg tgagctgcac
     1441 gaggaggtgt gccggctggc gaacgccctt aaggagctgg gcatctcgaa gggcgaccgc
     1501 gtcgcgatct acctcccgat gatccccgag cttccggccg cgatgctggc atgcgcccgg
     1561 atcggcgcgc cgcattcggt cgtcttcggg ggattctctg cagaatctct gcgcgatcgc
     1621 atcaacgatg ccgaagcgaa ggtccttatc accgcagacg gtggctaccg gagaggtgag
     1681 atcgttcctc tcaaggagaa cgccgaccac gcgctcgcag gcactcccag tatcgaaacc
     1741 gtcatcgtcg tgcgcaggac ggaacacgac gttccgtggc agcaggggcg cgatgtcgac
     1801 tacgcgcagc tagtggcttc ggctcccgtc gattgccccg ccgagccgat ggatgccgaa
     1861 gacatgctgt acatcctgta cacgtccggc accaccggta agccgaaagg gatcgtgcac
     1921 accacgggcg gctacctgac gggtgtagcg gcgacacacc gcctgatctt cgacatcaag
     1981 cccgaatcgg acgtgtactg gtgcgcggca gacatcggtt gggtgacggg ccactcctac
     2041 atcgtctacg gccccctcgt gaacggatgc acgtcgatcc tctacgaggg tgcacccgac
     2101 catcccgaca aggaccgatg gtggtcgatc gtcgaggagt acaacgtcac tatcctgtac
     2161 acggcaccta ccgcgatccg aaccttcatg aagtgggggc cggacttccc cgccaagcac
     2221 gacctgtcgt cgctccgtct gctcggaagc gtgggcgaac cgatcaaccc ggaggcatgg
     2281 atctggtacc aagagttcat cggctccggg cgaactccga tcgtcgacac ctggtggcaa
     2341 acggagacgg gtgcgatctg catcacgcca ctaccgggca taacaaccct caaacccggc
     2401 tcggcgacaa gaccattccc cggggtcttc
//
LOCUS       DATMED010000179         4871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5130291, whole genome shotgun sequence.
ACCESSION   DATMED010000179 DATMED010000000
VERSION     DATMED010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5130291"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..3082
                     /locus_tag="VNP73_04580"
     CDS             122..3082
                     /locus_tag="VNP73_04580"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HWL65231.1"
                     /translation="MKQILLYAFLAIPLIGAFSMFALGISVIYRASRVLNLSHGAMAM
                     LPAYAYYSLSEAGLPLIVSLPLSVAIGAILGVLVEFVFVRRLRPQGPTAQTVGTVAVT
                     GLLIALAAKVWGTTPLLPPGVFPEGQVDVAGAAVRYGDIGLFFVGIIVSGALFAFFKF
                     TEVGLAMRGAAQNRRGAALMGIDPDKTAAAAWALGGGLAGLAGILLASVTSLDPYNLS
                     LQVLPAFVAALIGGLESLPGALGGAAIAGLSFGLVPALAKLPGIGSVLRYSGATQLVL
                     TILALIVMAVKGRKFAGAEATESGLAGGSLASKPLKIGPGLLVLLLGLVIWPFFVPFS
                     VLGTSLLAVELALVAISLVVLIGWVGQISLAQASFVGIGALVTGMFTRGLGLGFPLNA
                     IAGAIVAGLAAAALGVVALRVRGLYLAVSTLIFAWMADQFLFPSPWLGSGTGSSTVPD
                     QILGTPGAFISFDFTNRKTLYFVFVAILALVAVLLANLRDTKTGRAFFAVRGSEIAAS
                     SLGIDITKYKLMAFAVSGLIAGLGGSLLMIEQRTVVPAQFLFTISLQYLAIVVVGGLG
                     SISGAISAGILFAALNELFFRVRVLSGWLEVVSAALLAVVLLVQPGGLAAAAAQSEKL
                     RLFIARYTAPLRKLLEPLTSGLAEKWRRTRSGVYSRPPAGDWFAMASAPAPDEEVAAP
                     KEEPPAKEEAEESAGLKLGAFTPVLKEMAPDRNDRTAFLEAAGITVRFGGLTAVSDAS
                     LVVREGEIVGLIGPNGAGKTTLFNAILGLNSPAAGTVSLHGRDATSLSPDLRARLGVA
                     RTFQVLQLFRELTVFDNLLVATHMHNASGIFSNLTVARKTIVAETEARQRVREIMSLL
                     KLEDVATETARNLPFGTLRMVELGRALVTGARLLLLDEIASGLNEAETDRLADVIRSV
                     RSLGVSVLLIEHDIRMVTSVSDYVYVLNQGQIIADGTSEEIQHNTEVIAAYLGEPATP
                     TSAQPEPGVVEAHA"
     gene            3075..3776
                     /locus_tag="VNP73_04585"
                     /pseudo
     CDS             3075..3776
                     /locus_tag="VNP73_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3947..4495
                     /locus_tag="VNP73_04590"
     CDS             3947..4495
                     /locus_tag="VNP73_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65232.1"
                     /translation="MRHLLKLAGTVIALMAFTTSAVAQECTPLPPKEVSGPILFSGNV
                     RQSVAATYFQNNACNWNGNDGLNGTDAQVLDLTGIEGQAAATISTGFPGFQIAFQGIV
                     LDGTCKKLGDTLSIGSSTIDFPVPYVIEVPAGAKWLIVQGSQASPAQTNFASQSTVTI
                     FFEGRECEAPPAPPKKKKRPPR"
     gene            4520..>4871
                     /locus_tag="VNP73_04595"
     CDS             4520..>4871
                     /locus_tag="VNP73_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65233.1"
                     /translation="MGRGVSTGRGLSVAIAVVTTALLFAATTASAQTPSPTPSGSGTP
                     SPSPSGSEGDAPTGPPSIALVNPSAGYDPRLDPGGADPPKVSDKDGPYQVVAWSANTP
                     QNAIAELYIRYPNSQ"
BASE COUNT          845 a         1528 c         1542 g          956 t
ORIGIN      
        1 cagcccgacc agccggttcg cgaacctgac gatgcagagc ttcgcgatcc agtaccgcgg
       61 cacattcgga ggctggcgcg ccggccggat cgtcaaagac gccggcgcgt agagcccagc
      121 gatgaagcag atactgctct acgccttcct agctattccc ctaatcgggg cgttctcgat
      181 gttcgccctg gggatctcgg tcatctatcg cgcgtcgcgc gtgctcaacc tgtctcacgg
      241 cgcgatggcg atgctgcctg cctacgcgta ctactcattg tcggaggccg gcttgcctct
      301 gatcgtttcg ctgcccttgt cggtggctat cggcgcgatt ctcggcgtcc tcgtcgagtt
      361 cgtcttcgtg cgacgcctgc ggccgcaagg tccgacggca cagacggtgg gaaccgttgc
      421 cgtcaccgga ttgttgatcg cgcttgccgc gaaggtgtgg ggaacgacac cactcctgcc
      481 cccgggcgtc tttcccgagg gtcaggtcga tgtcgccggc gcagcagtgc gctacggcga
      541 tatcggtttg ttcttcgttg ggatcatcgt ctccggcgcg ttgttcgcgt tcttcaaatt
      601 caccgaagtc ggattggcga tgaggggcgc tgcccagaac cgtcgcggtg ccgcgctgat
      661 ggggatcgac cccgacaaga ccgccgccgc tgcgtgggca ctgggcggag gtctggcagg
      721 actcgccggc atccttcttg cctcggttac gagcctcgat ccctacaacc tgtcgttgca
      781 ggttcttccc gccttcgtgg cggcgttgat cggtgggctc gagagtcttc cgggggcact
      841 tgggggggcc gcgatcgccg gactgtcctt cggactggtt cccgcactcg cgaagctccc
      901 ggggatcggt agcgtcctgc gctactcggg cgcgacgcag ctggtgctca cgatcctggc
      961 tctgatcgtc atggccgtga agggtcggaa gttcgcgggg gccgaggcca ccgagtccgg
     1021 tttggccggg ggttcgcttg cgtccaagcc gctaaagatc ggacccggcc tgctggtgct
     1081 cttgcttggc ctggtcatat ggccgttctt cgttccgttc tccgttctcg ggacctcgct
     1141 tctggccgtt gaactcgcgc tcgtagccat ctcgttggtc gtgcttatcg gctgggtcgg
     1201 acagatctca ctcgctcaag ccagcttcgt cggcatcggc gcgctggtaa cggggatgtt
     1261 cacgcgcggg ctcggcctcg gcttcccctt gaacgcgatc gccggagcga tcgttgccgg
     1321 tcttgccgcc gcggctttgg gcgtggtggc gctgcgtgtg cgcggcctct atctcgccgt
     1381 gtcgactctg atctttgcct ggatggcgga ccagttcctg ttcccatctc cgtggttggg
     1441 atcgggaacg ggaagctcga ctgtcccaga ccagatcctc ggaacgcccg gagcgttcat
     1501 ctccttcgac ttcactaatc gcaagacgct ctacttcgtg ttcgttgcga tcctcgcgct
     1561 cgtcgcagtt ctcctggcca acctgcgcga caccaagact ggtcgcgctt tctttgccgt
     1621 tcgaggttcg gagatcgcgg ccagctccct cggcatcgac atcacgaagt acaagctgat
     1681 ggccttcgcc gtctccggct tgatcgccgg gctcggtggg tcgttgctga tgatcgaaca
     1741 gagaacggtc gttccggcgc agttcctctt cacgatctcg ctgcagtacc tggcgatcgt
     1801 cgtggtcggt gggctcggca gcatcagcgg cgcgatctcg gcagggatcc tgttcgcggc
     1861 actgaacgag ctgttcttcc gcgtcagggt gctgagcggg tggctcgagg tcgtgtcggc
     1921 ggcgctcctc gcggtcgtgc tgttggtcca acccgggggc ctggccgctg ctgccgcaca
     1981 aagcgagaag ctcaggttgt tcatcgcgcg ctacaccgct ccactgcgca agctcctcga
     2041 accgctcacc tccgggctgg ccgagaaatg gcgtcggacc cgatccggcg tctactcgcg
     2101 cccgcccgcg ggcgactggt tcgcgatggc ttcggcccct gcaccagacg aggaagtcgc
     2161 ggcacctaag gaagagcctc cggcgaaaga ggaagccgag gagagtgccg ggctgaaact
     2221 cggagcgttc actcccgtac tgaaagagat ggcgccagat cgcaacgatc gcaccgcgtt
     2281 tctcgaggcg gcggggatca ccgttcgctt cggtggtctg actgcggtct cggacgcttc
     2341 tctcgtcgtg cgtgagggtg agatcgtcgg tttgatcggc ccgaacggcg ccgggaagac
     2401 gactctgttc aatgccatct tgggcctcaa ttcgccggcg gccggaaccg tgtctctcca
     2461 cgggcgcgat gcaacgtccc tgtcgccgga tctaagggct cggctcggcg tcgcccggac
     2521 cttccaggtg ctccaactct ttcgtgagct gaccgtgttc gacaacctgc tggtcgccac
     2581 gcacatgcac aacgcctccg ggatcttctc gaatctcacc gttgctcgaa agacgatcgt
     2641 cgcggagacc gaagctcggc aacgagtgcg cgagatcatg tcgctcctca aactggaaga
     2701 cgttgcaacg gaaaccgccc ggaaccttcc tttcggaacg ctgcggatgg tcgagttggg
     2761 acgggcactc gtgaccggag cgagacttct gctgctcgac gagatcgcgt ccgggctgaa
     2821 cgaggcagaa accgaccgac tcgccgacgt catcagatcc gttcgttcgc ttggggtctc
     2881 tgttctcttg atcgagcacg acatccgcat ggtgacgagc gtttccgact acgtctacgt
     2941 cctgaatcag ggccagatca tcgccgacgg aacgtctgaa gagatccagc acaacaccga
     3001 agtgatcgcg gcgtacctcg gagagccggc gacacccacg tcggcgcaac cagagcccgg
     3061 ggtggtggaa gcccatgctt gaggtcgagg ggatcgacgt ctattacggc gcggtacagg
     3121 cattgcgcgg gatcagcctg agcgtcgccg agggcgagat ggtgtcattg ctcggcgcga
     3181 acggtgccgg caagacgacg acgcttcgaa cgatctcggg cctgctcgcc ccccgcaacg
     3241 gaagtatccg tttcgaaggc gagagcatcg agggcaagcc ggcgcacgag gtagtcaagc
     3301 ttggtgtgtc tcagcttccg gaaggaagag agctcttcgc ggaactgacc gttgtcgaga
     3361 accttcgcct cggtcattgg tcacgcagga agaacaaggc ggagcgtgag gtcctcgtgg
     3421 aggagatctt cgagctgttt ccccgtttga gggaacgagc ttcgcaagcg gccggcacga
     3481 tgtcgggtgg cgagcagcag atgcttgcgg ccgcccgcgc actgatgaac aagcctcggc
     3541 tcctcctcgt cgacgagctc tcactcgggc tcgctccgat catcgtccaa cagctgttcg
     3601 aggcgatcgc cgaggtgaac cgccagggca ccgccgtgtt gctcgtcgag cagttcgtcc
     3661 atatggctct tcgctacacg acacgggcct acgttctcgc aaagggcgag gtcgtctccg
     3721 atggctcgag taaggacctc ctgggctcgc ccgacctgat ggccgcctac ctgggagtag
     3781 aggccaccgg gggagccccc tctaccaatg ggtccaaaaa accggacaaa gccaccgaat
     3841 cagacaccga tcgactacca aaagggaaag tcacccattc gggtgaagga acggggcatg
     3901 acacggacaa ccgagaggga agtagagaag gaaaaggagc gggactatga gacacctatt
     3961 gaagcttgct gggaccgtca ttgcactcat ggctttcacc acgtccgcag tggctcagga
     4021 atgcacgccc ttaccgccga aggaggtcag cgggccgatt ctcttttccg gcaatgtgcg
     4081 ccaatcggtc gccgcgacct acttccagaa caacgcttgt aattggaacg gcaatgacgg
     4141 cctcaacgga acggacgcgc aagtgctcga cctgactgga atcgagggcc aagccgcggc
     4201 tacgatctct accgggttcc cgggatttca gatcgcattc caagggattg tgctcgatgg
     4261 aacatgcaag aaactcggag acacattgtc gatcggaagc agcaccatcg acttcccggt
     4321 gccctacgtc atcgaggttc cggccggcgc gaaatggttg atcgtccagg gaagccaagc
     4381 ttccccggcg cagaccaact tcgcgagtca gtcgaccgtg acgatcttct tcgaaggcag
     4441 ggagtgcgag gcaccgccgg ctcccccgaa gaagaaaaag agaccacctc gctgatccgt
     4501 ctctcgagga attgaaagca tgggaagggg tgtgtcgacc ggccgcggtc tgagcgtcgc
     4561 gatcgccgta gtgactacgg cgcttctgtt cgcggcgacg actgcttccg ctcaaacccc
     4621 gtcacccact ccttccggga gtgggactcc ttctccttca ccctcaggga gcgaaggcga
     4681 cgcaccgacc ggtccaccca gcatcgcgtt ggttaaccca tcggcgggat acgaccccag
     4741 gctggatccc ggcggcgcag acccgcccaa ggtcagcgat aaggatggtc cgtatcaggt
     4801 ggtggcctgg tcggcgaaca caccacagaa cgcgatcgcc gagctctata tccgctatcc
     4861 gaactcccag g
//
LOCUS       DATMED010000180         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_901142, whole genome shotgun sequence.
ACCESSION   DATMED010000180 DATMED010000000
VERSION     DATMED010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901142"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VNP73_04600"
     CDS             <1..797
                     /locus_tag="VNP73_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWL65234.1"
                     /translation="NQQFGLRNVPKIVGGIDEASTQLMEAFYGSFVDVVVRVSSPAVA
                     ETAKLLENTFRHINIALVNEMAILCHDLGVDVWEVIDAAATKPFGFTPFYPGPGWGGH
                     CIPVDPAYLSWRVRQMGATAHFVELAREINARMPAYVVQRITECLNDQGKSLRASRVL
                     VLGVAYKPDVGDLRESPALEILEGLDKAGADITFHDPYVDEVQLKKRVIMRSELSPAS
                     LREADIVVAVTNHSGYDWGDIADRARTILDTRNALKAHPGPNVVRL"
     gene            complement(794..1909)
                     /gene="wecB"
                     /locus_tag="VNP73_04605"
     CDS             complement(794..1909)
                     /gene="wecB"
                     /locus_tag="VNP73_04605"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWL65235.1"
                     /translation="MADPIRVVIAAGARPNFMKVAPLMLALGSRPDVFETVLVHTGQH
                     YDAGMSDVFFEDLQMGSPDEYLNVGSGTHAEQTAAVLVAFEKVLAAAPPDIVLVAGDV
                     NSTLACALATRKLNVPVGHVEAGLRSRNWAMPEEVNRVVTDILSDYLFTPSPDANENL
                     EAEGISSDRVHLVGNIMIDSLARALPEAEKRQACSSVGLEPKTYGLITLHRPSNIDED
                     AQLKEILTSIGDAMPAAVFPVHPRTRKRITALEADGFSVAGTIKLLEPLGYYDFLSLM
                     MDASFVLTDSGGIQEETTYLGVPCMTLREETERPITVGEGTNELVTLERLPDAVASVI
                     KGDWKRGTIPDLWDGKTADRIVQILEKGIVPKLRGSG"
     gene            complement(1916..>2693)
                     /locus_tag="VNP73_04610"
     CDS             complement(1916..>2693)
                     /locus_tag="VNP73_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWL65236.1"
                     /translation="LDTDLLQKVLSNADSVVHLAARPSVPRSIADPVASHAANATGTI
                     SVLEAMRSAGVESIVAASSSSVYGSNPVLPKSEDLATLPMSPYAASKLATEAYTLAYQ
                     SSFGLKTMCFRFFNVFGPLQAAGHAYAAVVPAFVAAALAGEPLTVFGDGTQSRDFTFV
                     DTVGSVICEAVTKKMSHPQPVNLAFGTRVNLLKVIEMLEGILGRSLEVEHTEARKGDV
                     PHSQADQTQLRALFPTVEPVDLEAGLKATVDWFRATSGAS"
BASE COUNT          477 a          833 c          866 g          517 t
ORIGIN      
        1 gcaatcagca gttcggctta cgcaacgttc ccaagatcgt cggtggcata gacgaagcca
       61 gcacccagtt gatggaggct ttctacgggt ccttcgtcga cgtcgttgtt cgggtctcct
      121 cgcccgccgt cgcggagacg gccaaactgc tcgagaacac gttccgccac atcaacatcg
      181 cgctggtcaa tgagatggcg atcctttgtc acgacctggg tgtggatgta tgggaagtga
      241 tcgacgccgc cgccaccaag ccgttcggat tcactccctt ctatccgggt ccgggttggg
      301 gcggtcattg catcccggtc gaccccgcat atctgtcgtg gcgggttcgc cagatgggtg
      361 caaccgcaca ctttgtcgag ctggcacgcg agatcaacgc tcggatgccg gcttacgtcg
      421 tgcaacggat caccgagtgt ctgaacgatc aggggaagag cctgcgcgct tcgcgggttc
      481 tggtgctggg cgttgcttac aagccagacg tcggtgacct tcgtgagtcg ccggccctcg
      541 agatcctcga gggactcgac aaggccgggg cggacatcac cttccacgat ccctacgtgg
      601 atgaggtcca actaaagaaa cgggtcatca tgcggtccga attgagtccc gcttcgctcc
      661 gagaggccga catcgtcgtt gccgtgacga atcactccgg ttacgactgg ggggatatcg
      721 ccgaccgtgc gcgaacgatc ctcgatacgc gtaacgcgct caaggctcac cccgggccga
      781 acgtggttcg cctctagccg gatccccgga gcttcggaac gatccctttc tcgaggatct
      841 gcactatgcg atccgcggtc ttgccgtccc ataggtcggg gatcgtgccg cgcttccaat
      901 cgcccttgat gacggacgca accgcatcgg gtaggcgttc caaggtgacc agctcgttcg
      961 tgccctcgcc gacggtgatg ggtcgctccg tttcctcccg cagggtcatg cagggcacgc
     1021 cgaggtaggt cgtctcctcc tggatccctc ccgaatccgt aaggacgaac gacgcatcca
     1081 tcatgagact caagaagtcg tagtacccga gtggctcgag cagcttgatg gtgccggcca
     1141 ccgagaaacc gtccgcttcg agtgcggtga tccgcttccg tgtacgcggg tgcaccggga
     1201 agacggcggc cggcatcgcg tctccgatgc tcgtcaggat ctccttcagc tgagcgtcct
     1261 cgtcgatatt cgacggccgg tgcaacgtga tgaggccgta cgttttcggt tccaggccca
     1321 cggacgagca tgcctgtcgt ttctccgcct ctggaagcgc gcgcgcgagg ctgtcgatca
     1381 tgatgttccc gacaaggtgg acgcgatcgc tcgatatgcc ctcggcctcg aggttctcgt
     1441 ttgcatcggg ggaaggtgtg aacaaatagt ccgagaggat gtccgtgacc acgcggttca
     1501 cctcttcggg catcgcccag ttgcggctgc gcagcccggc ttcgacgtga cccaccggaa
     1561 cgttcagttt ccgagtggcg agcgcgcacg caagcgtgct gttgacgtcc ccggctacca
     1621 ggacgatgtc gggaggcgcg gcggccagga ctttctcgaa ggcaaccagc acggcggccg
     1681 tctgttcggc gtgcgttccg gaaccaacgt tcaggtactc gtcgggactg cccatctgga
     1741 gatcttcgaa gaacacatcc gacatgcccg cgtcgtagtg ctggcccgtg tgtacgagca
     1801 cggtttcgaa cacgtcgggc cgactgccga gcgccagcat caatggcgcc accttcatga
     1861 agttgggtcg cgctcccgcg gctatgacga cgcggattgg atctgccatg tccggctacg
     1921 acgctcccga ggtcgcgcgg aaccaatcca cggtcgcctt caatcccgcc tcgaggtcga
     1981 ccggctcgac cgtggggaat agtgcccgca actgcgtctg gtccgcctgc gagtggggga
     2041 catccccctt gcgggcctcc gtgtgctcga cctcgagcga ccggccgagg atgccctcga
     2101 gcatctcgat gaccttcaag aggttcacgc gcgtgccgaa cgcgaggttg accggttgcg
     2161 gatgcgacat cttctttgtg acggcctcgc aaatgactga tccgaccgta tcgacgaacg
     2221 tgaagtcgcg gctctgcgta ccgtcgccga agaccgtcaa tggttctccg gccagcgccg
     2281 ccgcgacgaa agcaggcacc acggcagcgt aggcgtggcc ggccgcttgc agggggccga
     2341 agacgttgaa gaaacggaag cacatggtct tgagcccgaa agacgactgg taggcgagcg
     2401 tgtaggcctc agtcgccagc ttggatgcgg cgtaggggct catgggcagc gttgcgagat
     2461 cttcgctttt cgggagcacc gggttcgatc cgtacacgga agaggacgag gcggcgacta
     2521 tcgattcgac tccggccgag cgcatggcct cgagcacgga gatcgtcccc gtcgcgttgg
     2581 ccgcgtgact ggcgacggga tctgcgatcg atcgcggaac ggaaggccgg gccgcgagat
     2641 gtacgaccga gtcggcgttg gaaagaacct tctgcaagag gtcggtgtcg agg
//
LOCUS       DATMED010000181         8385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_518993, whole genome shotgun sequence.
ACCESSION   DATMED010000181 DATMED010000000
VERSION     DATMED010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518993"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VNP73_04615"
     CDS             complement(<1..675)
                     /locus_tag="VNP73_04615"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWL65237.1"
                     /translation="MSDSSIGPRSAPVRSLLPEEFHELARRVNNWGRWGNDDEIGTLN
                     LITPELVSRAAGLVRSGRSISLALPLSEEGPQTGAVPGRTNPERTMTMVNDPVHPSSP
                     IRVSDDVVRMGLQAATHWDSLAHATYEGRMYNGFPPDSIDASGTTRCGIDKIKTLVGR
                     GVLLDVARARGVDRIEGAIPLTGDDLDAAAEFGRVKVGPADVVLIRTGQMQLFKEGDR
                     KAYASPA"
     gene            696..2825
                     /locus_tag="VNP73_04620"
     CDS             696..2825
                     /locus_tag="VNP73_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWL65238.1"
                     /translation="MANRNRLDVSGRPVELRDVELERFFNAQVIAVVGASDSPGKAST
                     LNYRLIRDWSKELGRRVYAVNPNRDQVDGEPCYSSVIDVPEDVDVVVILVSDVVGALR
                     QAIAKKAPFAVIFAAGFAESDADGAARQSEIEALIEDNEIHVLGPNTTLNAFQPFRRD
                     LKGKRIGLITQSGHQGRPIYQAQEIGIPMEGWAPTGNEADLESADFIRWFADQPDVGV
                     VAGYIEGFKDGRTLMLAANHALERGVPTVFVKSGRTEMGRSWVQTHAGHLAGSDSVMS
                     AVFRQFGVTRVDGLDELLDVSAMFARCPTPSGDGVAIYSISGGTCTHVADLATARGLR
                     VPDLTEETQARLHEWIPGNLRVSNPVDTGGHPTGDERGPKILKAILEDPNVSLLIVPV
                     VGSFSPISDRLAQDLVDAARNTKKPVCVVWGSPTADEPGYREVLLPSELPLFRSVGNC
                     LTAVKAYLDYHGFRDSYRSPFAKPVLRPLPAVRKVRDLLGTGGALSEHGSKQVLAEYG
                     IPVTRDILCGSAAEAARAAKSIGLPVVMKVSSQALLHKSDLGLVRLAVATEREVRSTF
                     AELMDQARGAAPDGPIEGVLVSELVDAGIETALGVVRDDLFGPTVMFGLGGIAIEVFR
                     DVSFRVPPFTKAEARRMIEETKSASLLRGARGKPKADLSALADAIMKVQRLAMDLSDE
                     IAELDVNPLMARPDGVIALDALVVASS"
     gene            complement(2822..3856)
                     /locus_tag="VNP73_04625"
     CDS             complement(2822..3856)
                     /locus_tag="VNP73_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HWL65239.1"
                     /translation="MNEPEELAPGETIPWLVHDLLDQQPEVSIGVILERTSVTRQAIQ
                     YHLKKMIESGELVPVGAGRGRRYRRPLLFEVTLETQGLQEDQVWKQVRSGVSELDELN
                     RNADRIANYAFTEMLNNAIEHSASDDVRITVGHQRDEFVFTITDQGIGAFENVRRQGD
                     LEDHVAAIQEISKGKMTTDPAHHTGQGIFFTSKAVNWFSIASNGWRWLVDNMREDQTI
                     ARSNVRRGTVVRFDLNKDTDRDLNKIFDEYTDEDSLAFDKSRTTVTLFEFGVPFVSRS
                     EAKRLARNLERFREVVVDFRGIEQVGQGFADELFRVWAGGHPETKLVPVNMDGPVKRI
                     VERALADAQH"
     gene            complement(5306..5692)
                     /locus_tag="VNP73_04630"
     CDS             complement(5306..5692)
                     /locus_tag="VNP73_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWL65240.1"
                     /translation="MHPNEERVRNAFDAYASKDIAGAQDLLDDNLTWHFAGKSPFSGD
                     YTGKDAVQDVWSRRREHTGGTLGYEVHDVLASDDHVVSLLLNKAERDGKTHEWKSVSV
                     YHMKDGKITEAWDFNEDQHAVDEFWS"
     gene            complement(5706..7235)
                     /locus_tag="VNP73_04635"
     CDS             complement(5706..7235)
                     /locus_tag="VNP73_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL65241.1"
                     /translation="MIEARTLWELIEARAEATPDGQALVDERGTSVSFAELKDRSERA
                     AAGLAARSVTEGTVVSWQLPTWNEAVVLVGALSRIGAVQNPILPILREREVGFIVRQA
                     QPRYLVVPSEFRGFDFQGMARTATEGSSTEVLVADRELPEADPSGLPAVPSTDGDLPV
                     RWLFYTSGTTADPKGAQHTDASVMEAALCMADGLDLQPDDRSALVFPFTHIGGIIWLF
                     AGLQVGFTNILQEAFDPVATTELLQKEGVTMAGAGTPFHMAYLNYRREHPDVDLFPNV
                     RAFPGGGAPKPPQLHHDIKKEIGGTGIISGYGLTEAPILTMGGVGDSDELLANTEGRP
                     RRGVQLRAVTLDGKEAGIGEEGEIRAKAPQLMQGYLDPALDQEAFDEDGWFRTGDLGK
                     LDADDNLTITGRLKDVIIRKGENISAKELEDLLFDHPKVADVAVIGLPDPEVGERACA
                     VIASKEGAQIQLEEVATFLMEKGLSKRKLPERVELVDALPRNPAGKVVKTELRDRYAN
                     S"
     gene            complement(7314..7853)
                     /locus_tag="VNP73_04640"
     CDS             complement(7314..7853)
                     /locus_tag="VNP73_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65242.1"
                     /translation="MRPQWRPTWRDYKLDAQIVESETTPMRVTEQSQLLRINDLIHDF
                     WFDYDEEDPESSGVKLDPTEELLEIEYRWEDWSNPRRDSGGFLRSTLLIPSFRGFLRV
                     FHVGEAQIHDRAQIGGSTFNVIEFEPSTSTVRIKTNIPIEISVVVSRLEVEVEITDES
                     TGEVTAKEYLGGLIQSGPN"
     gene            complement(7810..>8385)
                     /locus_tag="VNP73_04645"
     CDS             complement(7810..>8385)
                     /locus_tag="VNP73_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL65243.1"
                     /translation="IRRVDPASLDGRLVERPLDPLTPLLFVEHLPEGEQIDRPDAAAR
                     WRLEGAAMSAALLLGIALEATELATGYAKERVQFDRPIGSFQAVKHLLADMLVRAEVA
                     RAAVYAAGVVLDDPSAGDPARAIAAAKLLASEAAVTNGKTCIQVHGGMGFTWEAAAHL
                     YLKRASVLATTFGSSDEHAAAMAAHLAGLQT"
BASE COUNT         1563 a         2611 c         2549 g         1662 t
ORIGIN      
        1 ggcgggcgag gcgtaggcct tgcgatctcc ttctttgaac aactgcatct ggccggtgcg
       61 gatcaggacg acgtcagccg gtcccacctt cacgcgaccg aactccgctg ccgcatcgag
      121 atcgtctccc gtcagcggga tcgcgccctc gatccggtcc actcctcgcg cacgcgccac
      181 gtcgagcagc acgcccctgc cgaccaacgt cttgatcttg tcgataccgc atcgagtagt
      241 gccggacgcg tcgatcgaat cgggcgggaa gccgttgtac atgcggccct cgtaggtggc
      301 atgggcgagc gaatcccagt gcgtcgcggc ctggaggccc atccgcacca cgtcgtcgct
      361 gaccctgatc ggcgaagagg gatggacggg gtcattcacc atggtcatcg tgcgctcggg
      421 attggttctt ccgggaaccg caccggtctg cggtccttcc tctgagagag ggagggccaa
      481 cgagatgctc cggcccgacc gcaccagccc tgcagcgcgg ctgacgagtt ccggcgtgat
      541 gaggttgaga gtgccgatct cgtcgtcgtt tccccaccgg ccccagttgt tcacccgtcg
      601 ggcgagctcg tggaactcct cggggagaag ggaacggacg ggggccgaac gcggcccgat
      661 gctagaatct gacactccgt cagaaactac acgacatggc aaaccggaac cgactcgatg
      721 tcagcgggcg accggtcgag ctccgggacg tcgagctcga gcggttcttc aacgcgcagg
      781 tcatcgccgt ggtcggcgct tcggacagcc ccggcaaggc ttcgacgctc aactaccggc
      841 tgatacgcga ctggtcgaaa gagctcggcc ggagggtcta cgcggtcaat ccgaatcggg
      901 accaggtgga cggcgagccc tgctactcgt ccgtgatcga tgtccccgag gacgtcgacg
      961 tggtcgtgat cctggtgagc gatgtcgtcg gcgcgctccg acaggccatc gccaagaagg
     1021 ccccgttcgc agtcatcttc gcggccgggt tcgccgaatc cgatgccgac ggcgcggccc
     1081 gacagtcgga gatcgaagct ctgatcgagg acaacgagat acacgtgctc ggcccgaaca
     1141 ccacgctcaa cgccttccag cctttccggc gtgacctgaa agggaagagg atcggtttga
     1201 tcacccagag cggccatcag ggtcgcccga tctaccaggc ccaggagatc ggaatcccga
     1261 tggagggatg ggccccgacc ggcaacgagg ccgacctcga atcggccgac ttcatccggt
     1321 ggttcgccga ccagcccgac gtcggcgtcg tcgccggata catagaaggg ttcaaggacg
     1381 gacgcacgct gatgctggcc gccaaccatg ctctcgaacg cggcgtgccg actgtgttcg
     1441 tgaagtcggg acgaaccgag atgggccggt cttgggtgca gacgcacgcg ggccaccttg
     1501 ccggttcgga ctcggtgatg tccgcggtgt tccgccagtt cggcgtgacc cgcgtggacg
     1561 ggctcgacga gctgctcgac gtctcggcca tgttcgcgcg ctgcccgacg ccctcgggtg
     1621 atggcgttgc gatctattcg atctccggcg gcacctgcac tcacgtcgcg gatctagcca
     1681 ccgcgagggg gctgcgggtg ccggatttga ccgaggagac tcaagcgcgg ttgcacgaat
     1741 ggatccccgg caacctccgc gtctcgaacc cggtcgacac cggaggtcac cccaccggcg
     1801 acgagcgggg gccgaagatc ctgaaggcga tcctcgaaga tccgaacgtc agccttttga
     1861 tcgtgccggt cgtgggctcg ttctcgccga tcagcgatcg acttgcacag gacctcgtag
     1921 acgcggcgcg caacacaaag aagccggttt gcgtggtgtg gggctccccg accgcggacg
     1981 agccgggcta ccgcgaggtg ctgctgccgt cggagttgcc actattccga tccgtcggca
     2041 actgtctcac tgcggtgaag gcgtatctcg attatcacgg gttccgcgac agttaccgct
     2101 cgccgttcgc gaagcctgtg ttgcgaccac tccctgccgt gcgcaaggtg cgcgacctgc
     2161 tcgggacggg cggcgcgctt tcggagcacg gatcgaagca ggttctggcc gagtatggga
     2221 tccccgtcac gcgagacatc ctgtgcggaa gcgcggccga agcagcgcgc gctgcgaaga
     2281 gcatcggact ccccgtcgtc atgaaggtct cctctcaggc tctccttcac aagtccgatc
     2341 tcggcctcgt acggctggcg gttgcaacgg agcgcgaggt tcgttcgacg ttcgccgaac
     2401 tcatggacca ggcgcgtggc gccgcgcccg acggcccgat cgaaggcgta ctggtctcgg
     2461 agctcgtcga cgccggtatc gagacggccc tcggcgtggt ccgagacgat ctgttcggac
     2521 cgacggtgat gttcgggctg ggcgggatcg ccatagaagt gttccgcgat gtgtccttcc
     2581 gcgttccgcc gttcaccaag gcggaggccc ggcgcatgat cgaggagacc aaaagcgcat
     2641 cgctgctgag gggtgcgcgg ggcaagccga aagccgacct cagcgcgctg gccgacgcga
     2701 tcatgaaagt gcaacgcctg gcgatggacc tctcggacga gatcgccgaa ctggacgtga
     2761 atcccttgat ggcgcgcccg gacggtgtga tcgcgctaga cgcgctggtg gtcgcgtcta
     2821 gctagtgctg ggcgtccgcc aaggctctct caacgattcg ctttacggga ccgtccatat
     2881 tgaccgggac caacttggtc tcaggatggc ctcccgccca cacacggaac agttcgtcgg
     2941 caaagccctg acccacttgc tcgatccccc tgaagtcgac tactacttcc cggaaacgct
     3001 caagattcct agcaagacgc ttggcctctg aacgcgaaac gaaggggaca ccgaactcga
     3061 acaacgtcac tgttgttcgc gatttgtcaa aggccagtga gtcttcgtcg gtgtattcat
     3121 cgaagatctt gttcaaatcc cggtccgtgt ccttgtttag atcaaagcgc acgacggttc
     3181 cccttcgaac gttagatcgc gcgatggtct ggtcctctct catgttgtct acaagccatc
     3241 gccagccgtt gcttgcaatt gaaaaccagt tcacggcctt agatgtgaag aagatgcctt
     3301 ggccggtgtg gtgcgcagga tcggtcgtca tcttgccttt ggaaatctcc tggatagcgg
     3361 caacgtggtc ttccaggtca ccttgtctgc gaacattttc aaaggctccg attccttggt
     3421 cggtaatggt gaaaacgaac tcatcacgtt ggtgtccgac ggtgatccgc acatcgtcag
     3481 aggctgaatg ctcgatggcg ttgttaagca tctccgtgaa ggcgtaattc gcgatgcgat
     3541 cagcattccg gttgagctcg tcgagttcag agacgccgga ccgaacctgt ttccaaactt
     3601 gatcctcttg aagcccctgg gtctcgagcg tgacctcgaa caagaggggc ctgcggtacc
     3661 ggcgtccccg gccggcgccg accggcacga gttcgccact ctcgatcatc ttcttgaggt
     3721 gatactggat ggcctgacgc gtcacactcg ttcgttcgag gatgacgccg atcgagacct
     3781 cgggttgctg atcgaggagg tcgtgcacta gccacgggat cgtttccccc ggggcaagct
     3841 cttcgggttc attcattttg gtattttggc tttaatggcc ttaaatgtca aaaagccaag
     3901 acgcttgctc ggttttgttc tgcaaaccgg agccccgcgt agcgggtcaa acccaaggca
     3961 ccgacaccgc gtgtctgcgc gtcttccctg gtcagaagcc ctttctgagt ttgtggccgc
     4021 ctgcaaacga agcacccccc gccttggggg gtctggcact aaataccaaa acgccaaatc
     4081 tcacggaact ctaaatgcca aaagcacttg tcggagtaag taatcacctg gttgggttgg
     4141 tcaaacggtc actccggagg tccgatgaga cccttgaggg ccaaacgttg gggggacctc
     4201 cactgaagac tcgatcgttc gtggttgcct gcgctttgct tgctttgtac ttgcctcagc
     4261 cagccgtgca agcagacacc gtgtctattt ccgaccctca gggcgacaac tcgggaacta
     4321 acagatctga tgagcctgta accggcagac tagatatcgc caagttgact caggagcacc
     4381 aaccgccgag cccctcaagg gagcatgagc tcttggtgca tcgagtcacc acctacgacg
     4441 agtggtcgaa cgacctcatt tcatctggag acgtctttct ggatctctca tttgaaagtg
     4501 cgaaaggtca aacgggagtc tcgatcggtg tgaatgaaga cggcaccctc tatgggagag
     4561 cttccttcta caactatcgg cggcaggaat ccgccgcccg agggtttgct cgagtctggc
     4621 gtcccgattc gcggacgctc gttgtggctc tgtggcgaga tcaaatatgg cctggtcacg
     4681 cgaatgaact cacaaactac acatggacag ctcactccac ggatcgagac tgctcaaacg
     4741 tctctggagg agacgcaatc atcgcgtgca atgactatgg gcctgacgac ggtttccttc
     4801 acgtcatcga agggcctggg tccctagatt gccgagatgg tgttgacaat gacgacgacg
     4861 gttcgtcgga cttccctgaa gacactgggt gcatttcagc ggaggactat tacgaagggt
     4921 tgacggaaga agaatgtgat gaaccatgtg acccgcccac tctgaccatt aagaaacgat
     4981 ctgactcgct cagtgggtat atgcgatcgc cccaggaaac atgtcagaca gacagaacgg
     5041 tgttcctcta ccgaaaaagg tttggacctg acaggtgggt gatgagaata ggactcgatc
     5101 cgcaagggcg atgggggatg gacggccctg cccgccccgg tcgttactac gcagccgtct
     5161 tgccagagat caagtatccg atcggtgaac aagtcaaatg ttcagctgtg gcttcgcgca
     5221 ccgttcgttt cttgagcctt tagcaaaccg ttagcaactc gccgagccca cggatcagat
     5281 cagccacatc aggcctgttc agcgcctagg accaaaactc gtcgaccgcg tgctggtctt
     5341 cgttgaagtc ccacgcctcc gtgatcttcc cgtccttcat gtggtacacg ctgacgctct
     5401 tccactcgtg cgtcttgcca tcccgttccg ccttgttgag tagcagcgag acgacgtggt
     5461 cgtcgctcgc cagcacgtcg tgaacctcgt accccagcgt tcctcccgtg tgctcgcggc
     5521 gacgggacca cacgtcctgg accgcgtctt tcccggtgta gtcgcccgag aacggagatt
     5581 tgccggcgaa gtgccacgtc aagttgtcgt ccaagagatc ctgggccccc gcgatgtcct
     5641 tgcttgcgta agcgtcgaac gcgttccgca ctcgctcctc gttcggatgc acgtgtctcc
     5701 tccccttagc tgttcgcgta ccgatcccgg agttccgtct ttaccacctt gccagccgga
     5761 ttgcgcggca gagcatcgac caactcgacg cgttccggca gtttgcgctt ggacaggccc
     5821 ttctccatga gaaacgtagc gacctcctcg agctggatct gcgctccttc ttttgacgcg
     5881 ataacggcgc aggcgcgctc gcccacttcg ggatcgggga gcccgatcac ggccacgtcc
     5941 gccactttcg ggtgatcgaa cagaagatcc tcgagctcct tggcgctgat gttctcgccc
     6001 ttgcggatga tcacgtcttt gagccgcccg gtgatcgtga ggttgtcgtc ggcgtccagc
     6061 tttccgaggt cgccggtccg gaaccatccg tcctcgtcga acgcctcttg atcgagagcc
     6121 gggtcgaggt agccctgcat gagctgaggc gccttcgccc ggatctcgcc ctcctcgccg
     6181 ataccggctt ccttgccgtc gagggttacg gcgcggagct gcactccacg gcgcggtctc
     6241 ccctccgtgt ttgccagaag ctcgtcggag tcgcctacac cgcccatggt caggatgggc
     6301 gcttcggtca gcccgtagcc cgagatgatc ccggtccccc cgatctcctt ctttatgtcg
     6361 tggtgcagct ggggcggctt cggagcgccg ccgccgggga aggcccggac gttcgggaac
     6421 agatcgacat cggggtgctc gcgccggtag ttcaggtagg ccatgtggaa cggcgtaccc
     6481 gcccccgcca tcgtgacccc ttccttctgg agaagctctg tggtggcgac cggatcgaag
     6541 gcttcctgga ggatgttcgt gaatccaact tgcaacccag cgaacaacca gatgatcccg
     6601 ccgatgtgcg tgaacgggaa cacgagcgcg cttcgatcat cgggctgcag gtcgagtccg
     6661 tccgccatac acaacgccgc ctccatgacc gaggcgtcgg tgtgctgcgc gcccttcggg
     6721 tcggcggtcg tgcccgatgt gtagaacagc catcgcaccg gaaggtcgcc atccgtgctc
     6781 gggacggcgg gcaagcctga gggatcggct tccggaagct cgcggtcggc caccaacacc
     6841 tccgtcgacg agccttccgt cgcggttcgg gccatgcctt ggaagtcgaa gccacggaac
     6901 tcagagggaa cgacgaggta gcgcggctgc gcctggcgga cgatgaaccc gacctccctc
     6961 tcacgcagga tcggaaggat cgggttctgg accgcgccga tccggctcaa tgcaccgacc
     7021 aaaacgacgg cttcgttcca ggttgggagc tgccaggaga cgacggtccc ttcggtcacg
     7081 ctgcgcgcgg cgagacctgc ggcagcgcgc tcggagcggt ccttgagttc ggcgaaggaa
     7141 acggatgtac cgcgttcgtc gacgagcgcc tgtccatccg gcgtggcctc cgcgcgcgct
     7201 tcgatcaact cccagagagt ccgcgcctcg atcatcggta gcctctcatc tgacggtgcg
     7261 tcggataata accatgggta actatgaggt cctaataatg agaaaagaac atgtcagttc
     7321 ggtccagact ggataagccc gccgaggtat tccttcgctg ttacttcacc ggttgactcg
     7381 tcggtaattt cgacctcgac ttcaaggcga gagaccacaa cggagatctc gatggggata
     7441 ttcgtcttga tccgaaccgt ggacgtgctg ggttcgaatt cgatcacgtt gaaagtggac
     7501 ccaccgatct gggcccgatc gtggatttgg gcttccccga catggaacac cctgagaaaa
     7561 cctcggaagc tgggaatcaa caaggttgac cggaggaatc caccagaatc ccttcgggga
     7621 ttgctccaat cttcccatcg gtactcaatc tcaagaagtt cttctgtcgg atcaagtttg
     7681 acgccacttg actccggatc ctcctcgtca tagtcaaacc agaaatcatg aatcaagtcg
     7741 ttgatgcgga ggagctgact ttgctccgtc acccgcatag gtgtcgtttc cgattcgacg
     7801 atctgagcat caagtttgta atcccgccag gtgggccgcc attgcggccg catgctcgtc
     7861 gctagaaccg aacgtcgtag ccagaaccga agctcgcttc aagtagaggt gtgcggctgc
     7921 ctcccacgtg aaccccattc cgccgtggac ctggatgcac gtcttcccgt tggtcaccgc
     7981 cgcttcgctc gccaggagct tcgcggcggc gatcgcccga gcggggtccc ctgcggacgg
     8041 atcgtctagc acgacgccgg cagcgtagac ggcagcccga gccacctcgg cacggaccag
     8101 catgtcggcc aggaggtgct tcaccgcctg gaacgagccg atcggtcggt cgaactggac
     8161 ccgctccttc gcgtagccgg tcgcgagctc cgtcgcctcg agcgcgatgc cgaggagcaa
     8221 cgcggcgctc atcgccgcgc cttcaagtcg ccaccgagct gcagcgtcgg gccgatcgat
     8281 ctgctctccc tcgggcaggt gttcgacgaa cagcaacggt gtcaatggat ccagcggccg
     8341 ttcgacaagg cgtccgtcga gcgatgcggg atcgacacgc cggat
//
LOCUS       DATMED010000182         4003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4357080, whole genome shotgun sequence.
ACCESSION   DATMED010000182 DATMED010000000
VERSION     DATMED010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4357080"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..508)
                     /locus_tag="VNP73_04650"
     CDS             complement(95..508)
                     /locus_tag="VNP73_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWL65244.1"
                     /translation="MTLDPKAVVSRFYEIVNEGAVEKLDELCAPDMVGHAGAGADLAG
                     LKMSIGSFREAFPDLNAELRHVVAEDGLVSTWVSYEGTHQGDFAGVSGSDRPVKFVAW
                     DLIRVADGRIAEITMYCDVFTLMNQIGALPTAAPA"
     gene            complement(598..2286)
                     /locus_tag="VNP73_04655"
     CDS             complement(598..2286)
                     /locus_tag="VNP73_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWL65245.1"
                     /translation="MRLSEQASSGLISLEEGDWASAKRAFEDALEGDESPEVLDGLGR
                     SLWWLKDVRGAIELRTRAYAAYKKAGRIYEAAGLAVWLSRELRTLFRNDAAAEGWLAR
                     AETLAADTSESSIPGWVFLARAESCARASMAIADCGSALDMARDHGDSDLEIVTLARL
                     GLLEVATGDVDTGIRHLDEAMAAAGAGEADDLQSVGEAYCALMEAAEMLGDSGRFAQW
                     TSAISELRGEHGFGPLDDLGSSVAYGNLSAFCGACCGGMYLVTGHFDEAEDELLRAIA
                     DLEASGMESRCVHPVTQLAELRVLQGRFEEAQSLLEHYQDLPESVRPLAVLDLARRSP
                     DAAAARIQARLDELDDLTVATLPLQTVLVDAQLGRQDLDGAAIAAGRIGEIATLTKSK
                     RHLAEALFAKGKLAAARSEEDAPGILRDAARTFSEASLALPACRARMELAQTLVESDR
                     PVAISEARAALAAFERLGAVPDADAASAFLRDLGVKGRTGAKNLELLSKREHEVLRLV
                     AQGLSNPEIAERLFISTKTAGHHVSNILAKLGLRSRTEAAAYAAINLEREQARK"
     gene            2690..>4003
                     /locus_tag="VNP73_04660"
     CDS             2690..>4003
                     /locus_tag="VNP73_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HWL65246.1"
                     /translation="MAGVVAAALVASALVSSPATATPVKCEEGDVTPDDRVFPEPNNS
                     TSFLRFDEFECAIEFLERQHSDMIEVTTIGKSLGGRPIYDVLMTNEKVTTKKRKLLVV
                     NSIHGNEVGAREGAPRCIEDMVDERFLADEEWVKKVLDRYVIHWLFPNPDGWVAGDVT
                     GTEGASFMTTRGNDNGRDLNRQFPVKGYINRDNETYSEPESHDIVDALFGTKSKRKGW
                     YLGTDNHGQGADEYFAAGLQIVGQFNYQKSETLARFADGITETMKEYDVHGSVESLNE
                     QTGQDYGAYHWGTLYDMLGYSASGSLIDYYNTFDSTNGWGFATELTAGSNVNQLSHGQ
                     TVNQIYVDSIRAINYTMFRQAVAPKRFTYKVGGRAAYVWDPKVITDSDKNGAGFSDPQ
                     ATPEAKQRHFRVTRMKFFSDLNRFASRPLKKVRVGRVLKGTVNLRR"
BASE COUNT          774 a         1240 c         1244 g          745 t
ORIGIN      
        1 ttgtctcctt ccgatcgaac cgacctcggg cagctggacg tggcctgacg gattggagcc
       61 cggggttgct gtgtcttgct gtgtgctacg ggatctacgc gggggctgcg gtgggaagag
      121 caccgatctg gttcatgagc gtgaagacgt cgcagtacat ggtgatctcg gcgatgcggc
      181 cgtcggcgac ccggatgagg tcccacgcca cgaacttcac gggtcggtcg gatcccgaca
      241 ccccggcgaa gtccccctgg tgggttcctt cgtagctcac ccaggtgctg acgagtccgt
      301 cctctgccac gacatgacga agctcggcat tgagatcggg gaatgcttcg cggaagctgc
      361 cgatcgacat cttgagtcct gctaggtccg ccccggcacc ggcgtggccc accatgtcgg
      421 gagcgcatag ctcgtcgagc ttctcgaccg ctccttcgtt gacgatctcg tagaagcgac
      481 tcacgactgc cttcgggtcg agggtcatac cagtctcctt ttctccctgg ctcctttgga
      541 gcctctgctt cggattaccg tcgcgcaaga gggaagacaa aggatgggga gacttcccta
      601 tttcctagcc tgctctcgct ctaggttgat cgcggcgtag gcggcagcct ccgtccggct
      661 gcgcaaaccg agtttggcca ggatgttgct cacgtggtgg ccggcggtct ttgtgctgat
      721 gaacagccgt tcggcaatct cgggattcga caagccctgt gcgacgaggc gaagcacctc
      781 gtgttcacgc ttactcagaa gttcaaggtt tttggctccg gttcgtccct ttacgcccag
      841 atcgcgtagg aaggcagagg ccgcatccgc gtctgggacc gcgccgaggc gctcgaaggc
      901 cgctagcgct gcgcgggcct cggagatcgc tactggacga tcggactcaa cgagcgtttg
      961 ggccagctcc atccgagctc ggcaagcagg gagcgccaag gaagcctcgc tgaatgtgcg
     1021 agcggcgtcg cgcaggattc cgggcgcgtc ttcctccgat cgcgcggccg ccaacttgcc
     1081 cttagcgaag agggcctcgg caagatgcct cttgctctta gtcagcgtcg ctatctctcc
     1141 tatccgtcca gcagcaatcg cggccccgtc gaggtcttgt cggccgagct gcgcgtcgac
     1201 gagaacagtt tgcagaggca atgttgcgac ggtgagatcg tccagttcgt ccagtcgcgc
     1261 ttgtatccga gccgccgccg cgtccggtga tctacgtgcg agatcgagca ccgccagggg
     1321 ccggaccgac tcgggtaggt cttggtagtg ctcgagtagg gattgtgctt cctcgaagcg
     1381 gccctggagg actcgtagtt ccgcgagctg agtgaccgga tgtacgcagc gtgattccat
     1441 cccgctcgct tcgagatcgg cgatcgcccg cagcaactcg tcctcggcct catcgaagtg
     1501 cccggtcacg agatacatgc ctccgcagca agccccacag aatgcagaga gattcccgta
     1561 cgcaaccgat gagccgaggt cgtcgagggg cccgaaaccg tgctcgcccc tgagctccga
     1621 gatggcagag gtccactgcg cgaacctccc gctgtcaccc agcatctcgg cggcttccat
     1681 cagagcgcag taggcctcgc cgacactctg caggtcgtct gcctcccccg cgcccgctgc
     1741 cgccatcgcc tcgtcgagat gtcggatgcc cgtgtctacg tcgccggtcg cgacctcaag
     1801 cagtcctaga cgggcgagtg tgacgatctc gaggtcggag tcgccatggt cgcgggccat
     1861 atcaagggca gatccacagt ccgcgatcgc catcgatgcc ctcgcgcagg actccgcacg
     1921 tgcgaggaag acccaccccg ggatcgagga ttcgctcgta tcggcagcga gcgtctcggc
     1981 ccgcgccagc catccctccg ccgcggcatc gttgcggaac aaagttctga gctcgcggga
     2041 aagccacaca gcgagccctg cggcctcgta gatcctgccg gccttcttgt atgcggcata
     2101 cgcgcgcgtt cgaagctcga tcgcgcctcg gacgtccttc aaccaccaca aggagcggcc
     2161 gagtccgtcg agtacctcag gcgattcatc gccctcgagc gcgtcctcga aggcacgctt
     2221 cgcggaggcc caatcgccct cttcaaggga aatgaggccc gaagaggcct gctcagacag
     2281 tctcatcctt tgacatcatc cggctccctc cggctaagca caatgaggct ggaaaaatga
     2341 gcgtcgtcgg atgccgtggc tcgcgcttaa gagccgggcc gcagcgaatt gacccacccg
     2401 tacctgaccg gggaggatga atctcaagcg acggcgaggg ggaagacccc caagaggcag
     2461 cgggaggaat tcgaatgggc aacacgagac cggcgatccg cgagctgttt atcgaagagc
     2521 ttgttggcgt tcaaggcggg gcgagcacgg atatgagcaa cacgactatg gcgtgctgcg
     2581 aggaaggacc cattggttgt tgcgatcagg gcccagttga gtgggtcgaa aacatcatcg
     2641 accgcaccta atagtcgttt ctccacgtag ggggctcttc cggcacaggg tggccggcgt
     2701 agtcgcggct gctctcgtcg cttcggctct tgtcagctct cccgccaccg ctactccggt
     2761 caaatgcgaa gagggcgacg tcacaccaga cgaccgcgtc ttccccgagc cgaataacag
     2821 caccagcttt ctacgcttcg acgaattcga gtgcgctatc gagttcctcg aacgacagca
     2881 ctccgacatg atcgaggtaa cgacgatagg caagtccctc ggcgggcgtc cgatctacga
     2941 cgtgctgatg acgaacgaga aggtcactac gaagaagcgc aagctgctcg tggtcaattc
     3001 gatccacgga aatgaggttg gggctcggga aggtgctccg cgttgcatcg aggacatggt
     3061 cgacgaacgc ttcctcgccg acgaggagtg ggtcaagaag gtgctcgatc gttacgtgat
     3121 ccattggctg ttcccgaatc ccgacggatg ggtagccggg gatgtcacgg gcacggaggg
     3181 agccagcttc atgaccaccc gcggcaacga taacggccgc gatctcaacc ggcagttccc
     3241 ggtcaagggt tacatcaacc gagacaacga gacttattcg gaaccggagt cccatgacat
     3301 cgtcgacgct ctgttcggca ctaagagcaa acgcaagggt tggtatctag gtaccgacaa
     3361 ccacggtcaa ggcgcggacg agtacttcgc cgcgggtctg cagatcgtgg ggcagttcaa
     3421 ttaccagaag tcggagacgc tggctcgctt cgccgatggg atcaccgaaa ccatgaagga
     3481 gtacgacgtc cacggctcgg tcgagtcgct caacgaacaa accggtcagg actacggcgc
     3541 atatcactgg gggacgctct acgacatgct cggctattcg gcgagcgggt ctctgatcga
     3601 ttactacaac accttcgatt ccacgaacgg ctggggcttt gcgaccgagt tgacggccgg
     3661 ctcgaacgtg aaccaactga gccacgggca gacggtgaat cagatctatg tggactcgat
     3721 ccgcgcgatc aactacacga tgttccgcca ggcggtcgcg ccgaaacgct tcacctacaa
     3781 ggtgggaggt cgcgccgcct acgtctggga cccgaaagtg atcacagact cggacaagaa
     3841 cggggccggt ttttcggatc cccaagcgac accggaagcc aagcaacgcc atttcagggt
     3901 gacgaggatg aagttcttct cggacttgaa tcgctttgcc agccgcccgc tcaagaaggt
     3961 gcgcgtcggc cgggttctca agggcacggt caacctgcgc cgg
//
LOCUS       DATMED010000183         3170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3971016, whole genome shotgun sequence.
ACCESSION   DATMED010000183 DATMED010000000
VERSION     DATMED010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3971016"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VNP73_04665"
     CDS             <1..192
                     /locus_tag="VNP73_04665"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65247.1"
                     /translation="RLAIECQSYTWHSGQEAWERDVDRLSKLHRLGWIAHLVKNRDLD
                     EDPDGTLNRIWALYQQRAA"
     gene            complement(247..1182)
                     /locus_tag="VNP73_04670"
     CDS             complement(247..1182)
                     /locus_tag="VNP73_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWL65248.1"
                     /translation="MRTKRLGRDGPEISVVGFGAWEAGGSGWGEAPPDDQVIKAMRTA
                     IESGITWIDTAEVYGDGKSEELVGRALEPFKDALVFTKVAADNAGTGLKPEQVRAAAE
                     KSLKRLQRDVIDVFQIHWPDDSVPLEETWGAMAQLVDDGLVRWIGVSNFNQELIERCE
                     AIRHVDSLQNQFSMLRNKGRDDLFPFCEKNGTGIVCYGPLAYGLLTGAITTETEFDDD
                     DWRSGKGGLDHYYQPLFAPGALEENLAKVDALRPIADRLGVTLPQLALAWVVHQSGVT
                     GPIAGSRSPDHVAENAKAGSIELELKDLEEIDSIL"
     gene            complement(1179..1661)
                     /locus_tag="VNP73_04675"
     CDS             complement(1179..1661)
                     /locus_tag="VNP73_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWL65249.1"
                     /translation="MSPRVDVSMTDEEINRFLEVPRTGVLATLGRDGWPHPVGMWFAR
                     PDEESISMWAYRKSQKVVNLTRDPRCSFMVEHGESYDSLKGVLIRGRARLVDSEDDIY
                     SIGVALHERYTVPATGIAVEHGPEAVLRRQATKRTGIIVPIEQVASWDHSKLGGGGYT
                     "
     gene            1685..>3170
                     /locus_tag="VNP73_04680"
     CDS             1685..>3170
                     /locus_tag="VNP73_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65250.1"
                     /translation="MRRRIIPLSLAIVLLLAGVLYLRTRGAESPARRLDAGNRIFSSN
                     DPMKRACDLGEEVLIRLWRGHHPTHSEDITTVPQAPNYSGSFGVTSHSGPWDYVQTIP
                     LVLYGPGFVPDRGRIDEHASIVDVYPTISALLNVGLPAREGENLLGPTAPLPAPPKLI
                     VTVVWDGVGRNVLERWPDAWPNLARLEREGLSYLGATVGSSPSITPATHSSLGTGAFP
                     RSHGVTAIEYRTEEGDVRGAFAGKDPTDLKLSTFADEVDRLLDNEPLVGMLAWKSWHM
                     GMLGHGTQTEGGDGDELVLVNTHGDLTGNEALYTFPSGLESLEGRLEARAEQVDKADG
                     ESDGKTRGRDIIEMHDNPAWVYYQEDLLLGMLEQGGYGQDAVPDLFFTNFKVTDIVSH
                     QYSMDSKDEEIALRAQDATLGRLVEYLDKEVGDYVVIVTADHGNTPAPNTTGAWPIQQ
                     GQLQEDIDAHFEVPKGSSLIETTSAAGPFLDHATGEKLGIEPDDI"
BASE COUNT          630 a         1044 c          911 g          585 t
ORIGIN      
        1 agactggcga tcgagtgtca gtcgtacacc tggcactccg ggcaggaagc atgggaacgc
       61 gacgtggatc gcctctccaa gctccaccgg ctcggctgga tcgctcatct cgtgaagaac
      121 cgtgacctcg atgaggatcc ggacggcaca ctcaaccgca tctgggcgct gtaccagcag
      181 cgcgcggcct gaactctcca gaatccgacc gcctagaaag ggagccggat gtgcagaaag
      241 aacagtttag aggatggagt cgatctcttc taggtctttc agctccaact cgatcgagcc
      301 cgcctttgcg ttctccgcaa catgatcggg cgagcgcgag ccggcgatcg gtcccgtaac
      361 accactctga tgaacgaccc aagcgagagc cagttggggc aacgtcactc ccaaccgatc
      421 cgcgatcgga cgcagcgcgt cgaccttggc gaggttctcc tcgagcgctc cgggagcgaa
      481 caaaggttgg tagtagtggt cgaggccccc cttgccgctc cgccagtcat cgtcgtcgaa
      541 ttctgtttca gtcgtaatcg caccggtcaa caacccatac gccagaggtc cgtagcaaac
      601 gatccccgtc ccgttcttct cgcagaacgg aaagaggtcg tccctcccct tgttccttag
      661 catcgagaac tgattctgca gagagtcgac atgacggatc gcttcgcagc gctcgatgag
      721 ctcctgattg aagttcgaaa cgccgatcca tcgcacgagt ccgtcgtcta ccagctgagc
      781 catcgccccc cacgtctctt cgaggggaac ggagtcgtcg ggccaatgga tctggaacac
      841 gtcgatcaca tcccgttgca aacgcttgag gctcttctcg gcagcggctc tgacctgttc
      901 cggcttgaga ccggtgcccg cattgtcggc ggcaaccttc gtgaacacga gtgcgtcctt
      961 gaacggctcc aaggcgcggc ccaccaactc ttcggacttg ccatcgccgt acacctcggc
     1021 cgtatcgatc caggtgatcc cgctctcgat cgccgtacgc atcgccttga tcacctgatc
     1081 gtccggtggc gcttcgcccc aacccgagcc tccggcttcc cacgctccga aaccgacgac
     1141 ggagatctcc ggaccgtcac gacccaatcg cttcgttctc atgtgtatcc cccacctcca
     1201 agcttcgagt gatcccagga tgcgacttgc tcgatgggga cgatgatccc cgttcgcttc
     1261 gttgcctgcc ggcgtaggac cgcctccggc ccgtgctcca ctgctatacc cgtcgcgggc
     1321 acggtgtagc gctcgtgcag ggcgaccccg atcgaataga tgtcatcctc ggagtccacc
     1381 agccgagcac gacctcggat caacacgccc ttcaatgagt cgtacgactc gccgtgttcg
     1441 accatgaacg agcagcgggg atcccgagtc agattcacca ctttctgact ctttcggtat
     1501 gcccacatcg agatcgactc ctcgtcgggc cgcgcgaacc acatccccac agggtgcggc
     1561 catccatccc gccctaaagt ggccagcact cccgtcctgg gaacctccag gaagcggttt
     1621 atctcctcgt ccgtcatgga gacatcgact ctcggactca ccggcgcacc ttaaagtgcc
     1681 gcgcgtgcga cgaaggatca ttcccctatc cctcgcgatc gttctcctgt tagcgggtgt
     1741 gctttatctc cgcacgcggg gcgcagagag tcccgcgcga agactcgacg cgggcaaccg
     1801 gatcttcagt tcgaacgatc cgatgaaacg agcgtgcgac ctcggcgaag aggtccttat
     1861 ccgcctgtgg agggggcatc acccaacaca ttcggaagac ataacgacgg ttccacaggc
     1921 ccccaactac tccggctcgt tcggtgtgac gagccattcc ggtccgtggg actacgtgca
     1981 gacgatcccg ctggtgctgt acgggccggg cttcgtccca gatcggggtc ggatcgatga
     2041 acacgcaagc atcgtcgacg tctacccgac gatcagtgcg ttgttgaacg tcggactccc
     2101 agctcgagag ggcgagaacc tcctcggccc cactgccccg ctaccggcgc cccccaagtt
     2161 gatcgtcacc gtcgtttggg acggggtcgg tcgcaacgtc ttggaacggt ggccggacgc
     2221 gtggccgaac ctcgcgcgac tcgaacgcga aggcctctcc tacctcggcg caacggtggg
     2281 ttcgtctccc tcgatcaccc ccgccacgca ttccagtctc gggaccggtg cgttcccgcg
     2341 ctcgcacggt gtgactgcga tcgagtatcg aactgaggaa ggcgacgtac gcggcgcgtt
     2401 cgcagggaag gatccgacag atctgaaact gtcgaccttc gccgacgagg tcgatcgcct
     2461 gctcgacaac gagcccctcg tcgggatgct cgcctggaag tcgtggcaca tggggatgct
     2521 cggacacgga acgcaaaccg agggcggaga cggcgacgag ctcgtcctcg tcaatacaca
     2581 cggcgacctc accggcaacg aggcgctcta cacgttcccg tccggcctcg aatcgctgga
     2641 aggcaggctc gaggcacgag ccgaacaggt cgacaaggca gacggtgaaa gtgatggaaa
     2701 gacgcgcggt cgcgacatta tcgagatgca cgacaacccc gcctgggtgt actaccaaga
     2761 agaccttctt ttggggatgt tggaacaagg cggctacggg caggacgcgg tccctgacct
     2821 cttcttcacg aacttcaaag tgaccgacat cgtctcccac cagtattcga tggactcgaa
     2881 ggacgaggag atcgcgctcc gcgctcagga cgcgactttg ggcaggctcg tcgagtactt
     2941 ggataaggag gttggcgatt acgtcgtgat agtcaccgca gaccacggca acacccctgc
     3001 accgaacacc accggtgcgt ggccgatcca gcaagggcag ttgcaagaag acatcgacgc
     3061 tcacttcgag gttcccaaag gttcttctct gatcgaaacg acgtcggcag cggggccgtt
     3121 cttagaccac gccaccggtg agaagctcgg gatcgagccc gacgacatcg
//
LOCUS       DATMED010000184         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2825531, whole genome shotgun sequence.
ACCESSION   DATMED010000184 DATMED010000000
VERSION     DATMED010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825531"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VNP73_04685"
     CDS             complement(<1..254)
                     /locus_tag="VNP73_04685"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HWL65251.1"
                     /translation="MELDLGTARPLLEAWLREDIGHGDVTTGAVIPAGMLGRARIEAR
                     EPAVVAGLPLAGLCFEIVASTPLSWEAKVTDGSEVEAGEVL"
     gene            complement(301..1527)
                     /gene="ilvA"
                     /locus_tag="VNP73_04690"
     CDS             complement(301..1527)
                     /gene="ilvA"
                     /locus_tag="VNP73_04690"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWL65252.1"
                     /translation="MEAVASGGLVGLADIEEARHRLEGIAVMTPLDRSRALSDQVGGP
                     VLVKCENLQRTGSFKIRGAYNLISRLDADVRSRGVVAASAGNHAQGVALAASLQDARS
                     TVFMPASASLPKVEATKRYGAEVVLTGRDFGAAYAAACEFAEANDLFFVHPFDHPYVI
                     AGQGTIGCEIAEQAEEIGTVVVPIGGGGLISGVAAAFKRLRPEVRVIGVQAAGAAAFP
                     PSLEEGKPVTLEHMSTIADGIAANAPGELTLAHVREFVDEVVVVSDDAIAEALVFSAE
                     RMKMVLEPAGAAGVAALLQGVGHVTPPAVVVLSGGNIDPLLLLRVIRFGLSASGRYFA
                     FHTRIPDRPGELHRLLGLIAEIGGNVVGVEHHREGVSVHLGEVDVALQIETRGSEHIA
                     KLVEHLGDAGYTLERL"
     gene            1576..2934
                     /locus_tag="VNP73_04695"
     CDS             1576..2934
                     /locus_tag="VNP73_04695"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HWL65253.1"
                     /translation="MRAAREQEWQPDSWHGLPADQQPDWPDTGQLDAALSELRALPPL
                     VFAGEARSLQESLSQVAEGKAFLLQAGDCAEAFGDFSADAIRDKLKVILQMAVVLTYG
                     AGLPVVKVGRIAGQFAKPRSSPNERLEDQELPSFRGHAVNDIAFDPDSRKADPTRLVR
                     AYHQSAATLNLIRAFTKGGFADLNKIHIWNQEFVAGSKQGDRYEAIARQIDRALRFMA
                     ACGVDLERDPVLHEVDFYTSHEALLLGYEEALTRKDSLTDEWYDCSAHFLWVGDRTRS
                     PDGAHVEFLSGIKNPVAAKVGPSATPREVLDLCDRLDPDNEPGRLTLIARMGAERVEE
                     LLPPLIQAVTAAGRKVVWTCDPMHGNTFTTAAGIKTRRLDDIFTELKAFFAVCGQEGV
                     PPGGVHLELTHEAVTECLGGAEEVLDTNLSERYLALCDPRLNARQSLDLAFQLGEMLQ
                     QN"
     gene            3046..>3374
                     /locus_tag="VNP73_04700"
     CDS             3046..>3374
                     /locus_tag="VNP73_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65254.1"
                     /translation="MAEASARPSRTRLQRIVAGAILLVTCLSFPSPATGDETSIQDPS
                     DSPGRLDIITAGAGHDSSAEVLHTITFEDEDPPVEPEQIGLGLKYRGKRRVRLAHISI
                     KENPDGS"
BASE COUNT          638 a         1103 c         1050 g          583 t
ORIGIN      
        1 agcacttcgc ccgcttcgac ttcggagccg tccgtcacct tcgcctccca tgacaacggc
       61 gtagacgcga cgatctcgaa gcaaagccct gcaagtggga ggcctgcaac gaccgccggc
      121 tcgcgggctt cgatacgagc tcggcccagc atccccgccg gtatcaccgc acccgtcgtg
      181 acgtccccgt ggccgatgtc ctcgcgcagc cacgcctcga gaagagggcg ggcggtgccg
      241 agatccagtt ccatcaccat cactgtagat aacgactgag gtacttcgag ccttccctgt
      301 ttacagccgc tccagcgtgt agccggcatc gcctaggtgc tcgaccagtt tggcgatgtg
      361 ttcagacccc cgggtctcga tctggagggc cacgtcgacc tctcccagat gcaccgaaac
      421 gccttctcga tggtgttcga cgccgaccac gttcccgcca atctcggcga tcagtccgag
      481 caaacggtgc aattcacccg gtctgtccgg gatacgggtg tggaacgcga agtagcggcc
      541 ggaagcactc aatccgaagc ggatcactcg taagaggagg agggggtcga tgttcccacc
      601 cgacagcacg accaccgccg gcggggtgac gtggcccacc ccttgaagca gagcggcaac
      661 accggccgct cccgcaggct ccagaaccat cttcatccgc tcggcggaga acacgagggc
      721 ctctgcgatc gcatcgtcgg acacgacgac gacttcgtcg acgaactcgc gcacgtgggc
      781 cagcgtcaac tcgcccggtg cgttcgcggc gatcccgtcg gcgatcgtcg acatgtgttc
      841 cagcgttacg ggcttcccct cttccaacga aggcgggaac gcggccgcgc ccgccgcttg
      901 gaccccgatc actcgaacct ctggacgcag acgcttgaac gcggccgcca ccccagagat
      961 caatcccccg ccgccgatcg ggacgacgac ggttccgatc tcctcggctt gctccgcgat
     1021 ctcgcagccg atcgttccct ggcctgcgat gacgtaggga tggtcgaagg gatgaacgaa
     1081 gaagaggtcg ttggcctcgg cgaattcgca ggccgctgcg taagccgcac cgaaatccct
     1141 cccggtgagg acgacctccg ccccataacg cttcgtcgcc tcgaccttgg ggagcgaagc
     1201 cgaggccggc atgaacaccg tcgaccgggc atcctgcagc gacgcagcga gcgcgacacc
     1261 ctgggcgtgg ttgccggccg aagctgcgac cacaccccga ctgcgaacgt cggcgtcgag
     1321 ccgggagatg aggttgtagg cgccacggat cttgaacgag ccggtgcgct ggaggttttc
     1381 gcacttcacg agcacggggc cgccgacctg gtccgacagg gctctagacc ggtccagagg
     1441 cgtcatcacg gctatcccct cgaggcggtg gcgggcttct tcgatgtcgg ccagacccac
     1501 caggcccccg gacgcaacgg cttccatacg gcccatcgtc ccacggtgcc tcgctttaca
     1561 cgcgacgatt cccttatgcg tgcagctagg gagcaggagt ggcagccgga ttcgtggcac
     1621 ggcctgcccg cggatcaaca gcccgactgg cccgatacgg gtcagctcga tgctgcgctg
     1681 tccgagttgc gagcccttcc cccgctcgtg ttcgccgggg aggcacgttc gttgcaggag
     1741 tcgctgagcc aggtcgcgga gggaaaggca ttcctgctac aggccggcga ctgtgccgag
     1801 gcgttcggag atttctcggc cgacgcgatc cgggacaagc tgaaagtcat tctgcagatg
     1861 gcggtcgtcc tcacctacgg ggccgggctt cccgttgtga aggtaggtcg gatcgcggga
     1921 caattcgcga aaccgcgcag ctcacctaac gagcgactag aggaccagga gctgccttcc
     1981 ttccggggcc acgccgtcaa tgacatcgcg ttcgatcccg actcgaggaa ggccgacccg
     2041 actcgtctgg tgcgcgccta ccaccaatcc gccgcgacgc tcaatctcat ccgcgcgttc
     2101 acgaagggtg ggttcgcgga cctcaacaag atccacatct ggaaccagga gttcgtagcg
     2161 ggaagcaagc agggcgatcg ctacgaggcg atcgctcgcc agatcgaccg cgcgctcagg
     2221 ttcatggcgg cttgtggagt cgatctcgaa agggatcccg tgctgcacga ggtcgacttc
     2281 tacacatcgc acgaagctct tttgctcggt tacgaagaag cgctcactcg caaggacagt
     2341 ctcaccgacg agtggtacga ctgctcggca cacttcctgt gggtcggaga ccgcacacgt
     2401 tctcccgacg gtgcccacgt cgagttcctg tccggcatca agaacccggt cgcggccaag
     2461 gtcggcccca gcgcaacgcc gcgcgaggtc ctggatctct gcgacaggtt ggatcccgac
     2521 aacgagccgg gacggttgac cttgatcgcg aggatggggg cggaacgggt cgaggagcta
     2581 cttcctccac tgatccaagc ggtaacggcc gctggtcgga aggtcgtgtg gacgtgcgac
     2641 ccgatgcacg ggaatacgtt cacgacggca gcggggatca agacgcgccg tctggacgac
     2701 atcttcacgg aactgaaggc gttcttcgcc gtgtgtgggc aagagggggt gcccccgggc
     2761 ggggtccacc tcgagctcac acacgaagcg gtgaccgagt gccttggggg ggccgaggag
     2821 gtcctcgata cgaacctttc cgagcgttac ctggcgctgt gcgacccgag gctcaacgcg
     2881 cggcagtccc tggacctggc tttccagctg ggggagatgc tgcagcaaaa ctaaaccgtt
     2941 ctttcttaac atctggcccc ccgttcaggc gggcacattt agaaaagatc ggctgttcta
     3001 agtcccacag atccctaaat ccgatcactc tgtaatctca atcaaatggc ggaagcctca
     3061 gctcggcctt cacggactcg gctgcagagg atcgtggcgg gagcgatctt gctcgttacc
     3121 tgtttgtcgt tcccctcgcc ggcgacagga gatgaaacct cgatccagga tccgagcgat
     3181 tcgccgggga gactcgacat catcacggct ggcgctggtc atgattcctc cgcagaagtc
     3241 cttcacacga tcacgttcga ggacgaagac ccgccggtag aaccggagca gatcggtctt
     3301 ggacttaagt acagaggcaa gaggcgcgtt cggttggccc acatctcgat caaagagaac
     3361 ccggacgggt cctt
//
LOCUS       DATMED010000185         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_520552, whole genome shotgun sequence.
ACCESSION   DATMED010000185 DATMED010000000
VERSION     DATMED010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520552"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1549)
                     /locus_tag="VNP73_04705"
     CDS             complement(152..1549)
                     /locus_tag="VNP73_04705"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWL65255.1"
                     /translation="MATKTAPPKRLRVRPIDPALARFVRARMKDTGAPGVSVGVWHKG
                     RALGSGFGITSVEAPDPVDENTLFQIGSTGKTFTATAVMLLVDKGELDLDVPVRRYLR
                     DLKLKDADVTKKVTLRHLLTHTGGWAGDYFLDTGRGDDALAKAVASLHKVPQLTPLGE
                     VWHYNNAGFYIAGRLIEKATGMPYEKAVTELVLEPLGMTRSFFFAEETIGYRVVAGHN
                     AKTPRSTKHEMARPWDIGRNAGPAGGLISDVVDQMRWAQFHIGDGRAPNGKRLLKVST
                     MRQMQKPQAAAGNIAESVGISWLLDDYDGARTVAHGGTTYGQLSAFVMVPERDFAVTV
                     LTNSSRGMEVHGPVVRKALSLYLGLEQRLPVPIETNGSDLGRYEGRYVDAFKTAALDI
                     GVRGRRLHVNVGPVEGDTDLGLAPFYMAPFDEDMVLVQGGRLDGLRAEFLRDGRNRVR
                     WIRFGGRLWRKTPAR"
     gene            complement(1552..>2003)
                     /locus_tag="VNP73_04710"
     CDS             complement(1552..>2003)
                     /locus_tag="VNP73_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWL65256.1"
                     /translation="GQHALWRIFPDENLAVTLLTNGPGSREVFKDLFSEISGIKIPDQ
                     PKVPDDPPKLDLSRYAGSYERLAIRYDLAEEDGKLAGTVTVSGPLAKMIPDPVTHLSM
                     TPVDEKTFLVFSEDAGEEPGTAAFYDFVDGVPRYMHSGARANRRVGD"
BASE COUNT          369 a          666 c          602 g          366 t
ORIGIN      
        1 catcgatagc gatcttgtcg gctacctgtg cacatcccgt gcatgcaacc ggatccgagg
       61 acggggattc ggcctcaacg agcatccgaa gagcatcgac catctcggcc cggcggccct
      121 gaagatccga gagcaacgac atgaaaagag cttagcgagc gggtgtctta cgccataacc
      181 ggccgccgaa gcggatccat cgcacccggt tgcgtccgtc gcgcaggaac tcggcacgca
      241 gaccatctaa gcggccgccc tgcacgagaa ccatgtcctc gtcgaacggg gccatgtaga
      301 acggagccaa cccgaggtcg gtgtcgccct ccaccgggcc cacgttcacg tgcaacctgc
      361 gaccacgaac gccgatgtcg agcgcagccg tcttgaacgc atccacgtac ctgccctcgt
      421 agcgtccgag atcggagcca ttcgtctcga tgggcaccgg aagccgctgc tccagaccta
      481 ggtagagcga caacgccttc ctgacgaccg gcccgtgcac ctccataccg cggctcgagt
      541 tcgtaaggac ggtcacggcg aaatcgcgct cgggcaccat cacgaacgcc gacaactggc
      601 cgtacgtggt gccgccgtgc gcgaccgttc gcgctccgtc gtaatcgtcc aggagccacg
      661 agatcccaac cgattcggca atgttccctg ccgctgcttg tggtttctgc atctgacgca
      721 tcgtcgagac cttgagcagt ctcttgccgt ttggggcgcg tccgtcaccg atatggaact
      781 gagcccacct catttgatcg acgacgtccg agatcagccc tcccgccggg cccgcgttcc
      841 gaccgatgtc ccagggacgt gccatctcgt gcttggtcga ccggggcgtc ttggcattgt
      901 gaccggctac gacccgatac ccgatcgttt cctcggcgaa gaagaacgat cgggtcatgc
      961 cgagcggctc caggacgagt tctgtgacgg ccttctcgta tggcatcccg gtcgccttct
     1021 cgatcaaacg gccggcgatg tagaagccgg cgttgttgta gtgccacacc tcacccaggg
     1081 gcgtcagttg cgggaccttg tgaagcgacg cgaccgcctt cgcgagcgcg tcgtctcctc
     1141 tacccgtgtc caggaagtag tcgcccgccc atccaccggt gtgcgtcagc aggtgcctca
     1201 gcgttacctt cttcgtcacg tccgcgtctt tgagcttcag atcgcgcaga tagcgacgca
     1261 cgggaacatc gagatcgagc tcgcctttgt caaccagaag catcacggca gtagcggtga
     1321 acgtctttcc cgtggaaccg atctggaaca gggtgttctc gtcgacggga tcgggggcct
     1381 cgacgctcgt gatgccaaag ccggacccga gcgcccggcc cttatgccac acaccaacgc
     1441 tcacccccgg ggcgcccgtg tccttcatcc gcgcgcggac gaagcgggcc agtgcagggt
     1501 cgatcgggcg aacgcggagc cgcttgggcg gggccgtctt cgtggccaag ttcagtcgcc
     1561 gacccgccgg ttggcacgcg ctccgctgtg catatagcgc ggcacgccgt cgacgaagtc
     1621 gtagaaggca gccgtacccg gctcctcgcc cgcatcttcc gagaacacca gaaatgtctt
     1681 ctcgtccacc ggagtcatag acaagtgggt gaccggatcc gggatcatct tggcaagagg
     1741 tccactgacg gtgaccgtgc cggccagctt gccgtcctcc tctgccaagt cgtagcggat
     1801 ggcgagacgt tcgtacgacc cggcgtacct ggacagatcg agtttcgggg gatcgtcggg
     1861 aaccttcggt tgatcgggga tcttgatccc ggatatctcc gaaaacaggt ccttgaacac
     1921 ctcgcgagag ccgggcccat tcgttagcaa cgtcacggcc agattttcgt cggggaagat
     1981 ccgccacagc gcatgttggc cga
//
LOCUS       DATMED010000186         6835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3214725, whole genome shotgun sequence.
ACCESSION   DATMED010000186 DATMED010000000
VERSION     DATMED010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3214725"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1590
                     /locus_tag="VNP73_04715"
     CDS             154..1590
                     /locus_tag="VNP73_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65257.1"
                     /translation="MRKTLRLLLGPALIGSILAAPLGAAAGHQRPYVHVAGIQEAGGG
                     LEVVGHSPLNNRGMNAALAVHGDYAYVGSRTDAKAGNANGAGVLVVDVSKPEAPEVVH
                     EIGPPDEANPGETSREMRIWHEQELLIVMTLFSNCGEIHACSPIEGQDRFNFYDISGK
                     NAAAPKLVATYEPSENPHEFFLWEDPKDQGRALLFISNPGGGAQLTVTDISQARKGKF
                     TELLKMSPLYNGGNLHSLGISADGKRGYLAHLAGGVGVIDTSLVTKGAKKPAIKVLTL
                     AANAAQWPGPGPHSAVKIPGQDYMLITDEVYGAALKALGGHGCPWGWMRAVDIGNEAK
                     PKVVSEYKLPQNEESVCPPQDAIHPTTSFAAHNPTLTRNIAFITWHSGGLQAVDISDP
                     AKPAQLAEFVATPVVPILQEDPILSSGGDKVVAWSYPIIQDGLIYFVDVRNGLYVMRY
                     KGPYEKEVAAVDFLEGNSNQGDALKLDK"
     gene            complement(1661..2050)
                     /locus_tag="VNP73_04720"
     CDS             complement(1661..2050)
                     /locus_tag="VNP73_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWL65258.1"
                     /translation="MAVKFLTEEWASTMTEKLNASDEFKGAISGQSVKLQQVVTDAPE
                     GGETKYYFTLDGGQAEVSLGEIAEPDATITQNYETATAINKQELNAQNAFMQGKIKIS
                     GNMMKMMQLQGVFTAMPKAVDDVEVEY"
     gene            2110..3606
                     /locus_tag="VNP73_04725"
     CDS             2110..3606
                     /locus_tag="VNP73_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL65259.1"
                     /translation="MGRKAEGSAEAEPRAAATLVILREGVEGAEVLITIRPKELRFMG
                     GVAVFPGGAVAPEDSDPRWSRCSVLDGPTAATALGADPLEALAAYICAIRESYEEVAY
                     APVFSGAVPDRTSRPGAFLDSCLERNVVLQTDRLVPAGRWVTPLGSPVRFDARFFVTE
                     APGDWEPDPDPGEVAEAFWIAPSAALERWTEGELLMAPPTVEMLQRLALGSDVASILM
                     ELQGESSTEAGIETFSAFVRRVVAPNPGLMTGPGTNTYIVGAGPVCVIDPAVDDPAYI
                     DTLLSNHLDIDRILITHRHPDHVGGVAVLAERTGAEVRAFGDEEAGGVSVKPIVDEEV
                     IDVGGVELRALFTPGHAPDHLCFYLDGAASLFAGDNVLGFGTAVISPPEGNMKTYVES
                     LQRLRSLHISRIYPGHFPPLDGGDAIVEGYLQHRAERNEAIVAAVGSGASDIPEIVGA
                     VYTDVSSELHPIAEHQVRAHLEMLEQEGRVFRSGNRWLARGVYYSEGD"
     gene            3634..5790
                     /locus_tag="VNP73_04730"
     CDS             3634..5790
                     /locus_tag="VNP73_04730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWL65260.1"
                     /translation="MIPKRRSSISRGSWRWAAQVAELTAWGTAVLILLVLHVADLPDD
                     VYRLGLLLTGGLCLWFLVFFRFLLARRGPTWKAAWLGVVVDLGFAAAIFGLLRGQVAS
                     AQLIFVPVIMATGLLGTLPQALSATILAIAGYLGVSIASGSGGPTVITGAFNIAIFLL
                     SGSVAGLLARELRSHYRGEQEEHRIAIATRHRLFAVLDSIDEAIVYRDTHGVARVINH
                     RAAEIFGIVPSDFLGAPVVELLRNIARRTEDPEEFMETFQQVRDDPAIELRVPIDQII
                     PARRNLRMFSAPTFDDEGVLVGRIDVYTDVTENVRHAAEVARLYEEARKTAESYQRSL
                     LPDAPPSLPRVSLVAHYLPAAGRRAVCGDFYDFVPFSDGRVGLTLGDVTGIGPRAAAD
                     AALTRYTLRSLASQERDPARLLARMNKQIYMQASGERFVRLLLAVLDPERAVLTYANA
                     GHVPPILYRARTGEIEWLGEGGLVLGVEVDSEYKVGEISLDPGDMVVLYTDGLTEAPR
                     FGRPYGQGRLSDMVTDYGMGTPGELVQALRRSVESWVAETELRDDLAIVVCQVAPDRT
                     LGEPARELVLPNETVRVPEVRRFVASFLADVGASVEISSEVLLAVGEAAANATRHGRS
                     PDTRSEIRVRCSLEGPSVSISISDDGPGFDPAALDVATLPDRFASGGRGLFLMRSLMD
                     EVEVDSSEMGTEVTMFRRIFGASEKPELSEAGPAQS"
     gene            5811..>6835
                     /locus_tag="VNP73_04735"
     CDS             5811..>6835
                     /locus_tag="VNP73_04735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWL65261.1"
                     /translation="MAPIQPQRRLVMAREAVIVEAVRTPLGKRNGKLKDWHPVDLLGV
                     TLKELIERSGVDAATVDDVVCGCVSQVGEQSLNVGRNAWLASGLPEEVPATTVDRQCG
                     SSQQAAHFAAQGVIAGAYDLAIACGVESMTRVPMGTSAQTGGFPFPDSVMSRYEGGLV
                     PQGTSAEIISERWGLGREELDRFGARSHERAGAAISSGAFKKEIFPIKVETESGTELF
                     ETDEGVRTPNLEKMAQLQPAFKEDGVVTAGNSSQITDGASALLVTTPEKAKELGLTPR
                     ARFVSFALAGTDPVTMLTGPIPATKKVLEKAGLSIEDIDLVEINEAFASVVLAWKKDL
                     GLDDQWF"
BASE COUNT         1191 a         2111 c         2236 g         1297 t
ORIGIN      
        1 gggggagggc aacaagctca gcaatagagc cagcacgagg atctttcgca taccggttga
       61 ttcgccgccg gcagtcccgt ccctccgtgc ctagagagga gtgaaggggc gggtggcgaa
      121 cctctcattc cagagaatga aggggagata gacatgcgga aaaccttgcg acttttgctt
      181 gggccggctc tgatcggttc gatcttggcc gctccactgg gagctgccgc cggccaccag
      241 aggccttacg tccacgtagc cggcatccag gaggccgggg gcggattaga ggtcgtcggc
      301 cattccccgc tcaacaaccg cgggatgaac gcggccctgg cggttcacgg cgactacgcc
      361 tacgtcggca gccgtaccga cgcgaaggcc ggcaatgcga acggagccgg ggtcctcgtg
      421 gtggacgtct cgaaacccga agctcccgaa gtcgtgcatg agatcggtcc acccgacgaa
      481 gctaatcccg gcgagacgtc gcgggagatg cggatctggc acgagcagga gctgctgatc
      541 gtgatgaccc tgttctccaa ctgcggtgag atccacgcgt gttcaccgat cgagggtcaa
      601 gatcggttca acttctacga catcagcggc aagaacgcgg ccgcgcccaa gctcgtcgcg
      661 acctacgagc catcggagaa cccgcatgag ttcttcctgt gggaggaccc gaaggaccaa
      721 ggtcgcgcgc tgttgttcat ctcaaacccg ggcggtggcg cgcagctcac ggtcacggat
      781 atatcgcagg cacgtaaggg caagttcacc gagctcctga agatgtcgcc gctttacaac
      841 ggtggcaacc tccactcgct cggtatctcc gccgacggga aacgcggtta cctcgcgcac
      901 cttgcgggtg gcgtcggcgt gatcgataca tcactggtga ccaagggcgc taagaagccc
      961 gcgatcaagg tgcttacgct tgccgccaac gcggctcagt ggccgggacc cggcccccac
     1021 agcgcggtca agatccccgg tcaggactac atgctgatca cggacgaggt ttacggtgca
     1081 gcgcttaaag cgctcggtgg ccacgggtgc ccgtggggat ggatgcgcgc agtcgacatc
     1141 gggaatgagg ccaagccgaa ggtggtttcc gagtacaagc ttccgcagaa cgaagagtcc
     1201 gtgtgcccac cgcaggacgc gatacatccc acgacgtcgt tcgcggctca caacccgacg
     1261 ctgacgcgca acatcgcatt catcacgtgg cactcgggcg gtctacaggc tgtcgacatc
     1321 tctgatccag cgaagccggc ccagctcgcc gagttcgtag ctaccccggt ggttcccatc
     1381 ctgcaggaag atccgatctt gtcgtcgggc ggcgacaaag ttgtggcttg gagctacccg
     1441 atcatccagg acggactcat ctacttcgtc gacgttcgca acggcctcta cgtgatgcgt
     1501 tacaagggac cgtacgagaa agaggttgcc gcggtcgatt tcctcgaggg caactcgaac
     1561 caaggggatg cactgaagct cgacaagtag agtcgttagc gctagatcga tgcgagggcg
     1621 cggtggctta gccaccgcgc cgctaggtaa tcagttcgaa ctagtactcg acctcgacgt
     1681 cgtctacggc cttcggcatc gccgtgaaga cgccctggag ctgcatcatc ttcatcatgt
     1741 ttcccgagat cttgatcttg ccctgcatga aggcgttctg ggcgttgagc tcctgcttgt
     1801 tgatcgccgt tgccgtctcg tagttctggg tgatcgtggc gtcgggctcg gcgatctccc
     1861 cgagggagac ctcggcttgg cccccatcga gggtgaagta gtacttggtc tcgcccccct
     1921 cgggagcatc ggtaacgact tgctggagtt tcacggactg gccggagatg gcccccttga
     1981 attcgtccga ggcgttcagc ttctcggtca tcgtggatgc ccattcttcg gttaagaact
     2041 tcaccgccat gtatttcctc cttgcaggct ggtttgaacg aagcgccgag aacataccgt
     2101 tttccgcaca tggggcgtaa agcggagggg tcggcggagg cggaaccgcg tgcggccgcg
     2161 accctcgtca tcctccgcga gggcgtcgag ggagccgagg tcctgatcac gatcaggcca
     2221 aaagagctgc gcttcatggg cggcgtcgcg gtctttcccg ggggcgccgt cgctccggag
     2281 gactcggatc cccgatggtc tcgatgctcg gtcctcgacg gtcccaccgc tgccaccgct
     2341 ctcggcgccg acccgctgga ggcgctggcc gcatacatct gcgccatcag ggagtcctac
     2401 gaggaggttg cctacgcgcc ggtgttctcg ggagccgtgc ccgatcggac gtcccgtcct
     2461 ggcgcgttcc tcgattcatg cctagagcga aacgtggtgt tgcagaccga ccgactcgtg
     2521 cccgccggac ggtgggtcac gcctctcggc tcgccggttc gattcgacgc gcgcttcttc
     2581 gtcacggagg ctccgggcga ctgggagccg gatcccgacc ccggcgaagt ggccgaggcg
     2641 ttctggatcg ctccatcggc cgcgctcgag aggtggaccg agggagagtt gctgatggct
     2701 ccccctacgg tcgagatgct tcagcgactc gcactcggat ccgacgtagc ttcgatcctg
     2761 atggagttgc aaggggagtc ctcgaccgaa gccggtatcg aaaccttctc tgcattcgtc
     2821 cgcagggtgg tcgccccgaa cccggggctc atgacgggac cggggacgaa tacatacatc
     2881 gtcggcgcgg ggccggtctg tgtcatcgat cccgcggtcg acgacccggc ctacatcgat
     2941 acgttgctga gcaatcacct ggacatcgat cgcatcctga tcacccatcg tcacccggat
     3001 cacgtcggcg gggtcgctgt tctcgcagaa agaacggggg ccgaggtgcg cgcgttcggc
     3061 gacgaggagg ccggcggagt ctccgtcaaa ccgatcgtgg acgaagaggt gatcgacgtc
     3121 ggaggtgtgg agctgcgcgc gctgttcaca ccgggacacg cccccgacca tctctgcttc
     3181 tacctcgacg gcgcggccag tcttttcgcc ggagacaacg tgctcgggtt cggaacggcg
     3241 gtcatctcgc cgcccgaagg gaacatgaag acatatgtcg agagcttgca gcgcctacga
     3301 tccctgcata tctcgcgcat ctatcccggc cacttcccgc ctcttgacgg tggagatgca
     3361 atcgtcgagg gctacctgca gcaccgcgcc gagcgcaacg aggcgatcgt cgcggcggtg
     3421 ggttctgggg cttctgacat ccccgagatc gtcggagccg tctatacgga cgtatccagc
     3481 gagcttcatc cgatcgcgga gcatcaggtg cgtgcacatc tcgagatgtt ggagcaggaa
     3541 ggacgagttt ttcggtccgg taaccgttgg ttggcccgtg gcgtttacta cagtgagggg
     3601 gactgagccg gaggacgtga ccaaggaccg gggatgattc ctaagcggcg cagctccatc
     3661 agcagaggca gctggagatg ggcagcgcag gttgccgagc tgactgcgtg gggcacggcc
     3721 gttctgatcc tcctggtcct tcacgtggcc gatcttccgg acgacgtcta tcgcctggga
     3781 cttttgttga ccgggggtct gtgcctctgg ttcctcgtct tcttccggtt cttgcttgcc
     3841 cgcaggggtc caacctggaa ggcggcgtgg ctcggcgtcg tcgtcgacct tggtttcgcg
     3901 gccgcgatct tcggtttgct gcgcggacag gtcgcctcag cgcaactgat ctttgtcccg
     3961 gtgatcatgg ccacgggtct cctcgggact ctcccacaag ccctctcggc aacgatcttg
     4021 gcgatagccg gctatctcgg cgtctcgatc gcctcgggtt ccggcggccc taccgtcatc
     4081 acgggagcgt tcaacatcgc gatcttcttg ttatcgggat ccgtcgcggg cctgttggcg
     4141 cgcgagctcc gctcgcatta ccgcggcgag caggaagagc atcggatcgc catagcgacg
     4201 cgccaccgct tgttcgccgt tctggactcg atcgacgaag cgatcgtcta tcgcgatacg
     4261 catggcgttg cgcgtgtgat caaccatcga gcggcagaga tcttcgggat cgttccctcg
     4321 gacttcctcg gcgctccggt ggtcgagcta ctgagaaaca tcgctcgtcg cacggaagac
     4381 ccggaagagt tcatggagac gttccaacag gttcgtgacg atccggcgat cgagttgcgg
     4441 gtgccgatcg accagatcat cccggcacgg cgcaacctgc gcatgttttc ggcccccacc
     4501 ttcgacgacg aaggtgttct cgtcggccgg atagacgtct acacggacgt aaccgagaac
     4561 gtcaggcatg cggccgaggt cgcgaggctc tacgaagaag cacgtaagac cgccgagagc
     4621 taccagcgca gcctcctccc cgatgccccg ccgagcctcc cgcgggtgtc gctggttgcc
     4681 cactacctgc cggcagccgg tcggcgggcg gtgtgtgggg atttctacga cttcgtgccg
     4741 ttcagcgacg ggcgcgtcgg cttgactctc ggcgatgtca ctgggatcgg accgcgtgct
     4801 gccgccgatg cggcgctcac ccggtacacg ttgcggtcac tcgccagtca ggaacgagat
     4861 ccggcgcgtc tcttggcgcg gatgaacaag cagatctata tgcaggcttc cggcgagcga
     4921 ttcgtgagac tgctgctggc cgtcctggac cccgagcgag cggttcttac gtacgcgaat
     4981 gcaggtcacg tgccgccgat cttgtatcga gcccgcacgg gggagatcga gtggctcggt
     5041 gaagggggcc tcgtcctcgg ggtcgaggtc gacagcgaat acaaggtcgg cgagatctca
     5101 ctggatcccg gcgacatggt cgttctctac acggacgggc tgacggaggc gccgcgcttc
     5161 ggcagaccgt atgggcaggg ccgtttgagc gacatggtga ccgactatgg gatgggcaca
     5221 ccgggagagc tcgttcaggc cctgagacgt tcggtcgagt cgtgggtcgc cgagaccgag
     5281 ctacgcgacg accttgcgat cgtcgtctgc caggtggcgc ccgacaggac gttgggcgag
     5341 ccggcgcgcg agctcgtgct gccgaacgaa acggtccgtg ttccggaggt ccgcaggttc
     5401 gtcgcctctt tcctggccga cgtcggcgca tcggtcgaga tctcgtcgga ggtcctcctg
     5461 gcggtgggcg aggccgcggc gaacgccacc cgtcacggta ggagtccaga tacccgcagc
     5521 gagatccggg tgcggtgctc gctggaggga ccgagtgtct cgatctccat ctccgacgac
     5581 ggtcccggtt tcgaccctgc ggctctcgat gtcgcaacgc ttccggatcg gttcgcgtcc
     5641 gggggccgcg gcctgttcct tatgcgctca ctgatggacg aggtcgaggt cgattcctcc
     5701 gagatgggaa cggaagtgac gatgttccgc cgcatcttcg gtgcgagcga gaagccggag
     5761 ctgtcggagg cagggcccgc tcaaagctga gatacttgac ccttcggtca atggcaccca
     5821 ttcagccgca aaggagactc gttatggcgc gtgaggccgt catcgtcgaa gcagttcgga
     5881 ccccgttggg caagcgcaac gggaagctga aggactggca tccggtcgat ctactcgggg
     5941 tcactctcaa agaactcatc gaacgatctg gtgtggatgc cgccacggtc gacgacgtcg
     6001 tttgcggctg cgtcagccag gtcggcgagc aatctctgaa cgtcggtcgc aatgcatggc
     6061 tggcttcggg gctgcccgag gaggttccgg ctacgacggt cgaccgtcag tgcggctcct
     6121 ctcagcaggc cgcccacttc gccgcgcaag gtgtgatcgc cggggcgtac gacctcgcga
     6181 tcgcttgcgg agtcgagagc atgactaggg ttcccatggg cacgagtgcc cagaccggcg
     6241 gattcccttt ccccgacagc gtcatgagtc gctacgaagg tgggctcgtt ccccaaggca
     6301 cgtctgccga gatcatctcg gagcggtggg gactcggccg tgaagagctg gaccgtttcg
     6361 gcgcccgctc gcacgagcgc gccggtgcgg cgatctcttc gggcgcgttc aagaaggaga
     6421 tcttcccgat caaggtcgag acggagagcg gcaccgaatt gttcgagacc gacgagggcg
     6481 tccgcacgcc gaacctcgag aagatggccc agttgcagcc ggcattcaag gaggatggtg
     6541 tcgtcacggc cggcaactca tcccagatca ccgatggagc atcggctctt ctggtcacga
     6601 ctccagagaa agccaaggag ctcgggctga ctccgcgtgc tcgtttcgtt tccttcgcac
     6661 tcgccggcac cgatccggtc acgatgctca ccggtccgat ccctgccacc aagaaggtgc
     6721 tcgagaaggc cgggctctcg atcgaggaca tcgatctcgt cgagatcaac gaagcgttcg
     6781 cgtcggtcgt gctcgcgtgg aagaaggacc tcgggctcga cgatcagtgg ttcga
//
LOCUS       DATMED010000187         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3976088, whole genome shotgun sequence.
ACCESSION   DATMED010000187 DATMED010000000
VERSION     DATMED010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3976088"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VNP73_04740"
     CDS             complement(<1..301)
                     /locus_tag="VNP73_04740"
                     /inference="COORDINATES: protein motif:HMM:NF039255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein pafC"
                     /protein_id="HWL65262.1"
                     /translation="MPEDPSGRLGRRLSRILLLLPYAIQHPGVTVGELAGKFGIDKRD
                     LLDDLNLVFLCGLPGYGPGDLIDVDLDEDRVYVRMADYFAAPLRLAPAEALVLYAG"
     gene            complement(294..1250)
                     /locus_tag="VNP73_04745"
     CDS             complement(294..1250)
                     /locus_tag="VNP73_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWL65263.1"
                     /translation="MRRIERLINLIAALLETQRPLTAEDIRRQIAGYDQDNREAFRRA
                     FERDKEALRSMGIPLELVTTDPLGDDGDGYVIPKEKYYLPELDLEPDELAALSIAAGA
                     VLGAGPQAASGMMKLSLDLPQEGWNGPRVVWGTDLAAEQPLLGPLYAAVSEREAISFE
                     HAGTDGTVVMRELEPYGLVHRRGNWYVVGRDTSKDAIRGFKVSRLGGTVERLGRTFSP
                     PEDFRPADHLDLEAWEVGPEPVPARVRFGPDFRWWAEQNMAEPKTAGPDGSLDIELSV
                     ANADALISWVIGFGGRVQIVSPAELRKKLVDHVAPFAVRADA"
     gene            complement(1300..2367)
                     /locus_tag="VNP73_04750"
     CDS             complement(1300..2367)
                     /locus_tag="VNP73_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HWL65264.1"
                     /translation="MAAFRAGKVVSIESEGPGLVKVRVAIGADEVPASGYPAMVGQLN
                     VGDRVVVNTTGLELGLGTGGEGFVLWNLDGPEIEPGEGHIVKMRYTPWQTNVLTAEAP
                     ESEHHMSLVDADDLDGTPVVACGLHSQIAAVAAGIKAERPETRVAYVMSDAGSLPIAW
                     SNLVRELRESGVIDLTCTYGHAFGGDLEAVNVYSALVATKMVGRADAIVVAMGPGGVG
                     TGTRLGYSGIEQAQVLDAAAALGGSAIAALRISFVDARDRHQGISHHSITSIGLVARR
                     VTIVLPELPEERGALVRTQLKTVASQDVVIADGRPGLDLLRERGIDPSTMGRSMSESP
                     EPFLAASAAGRIAAQRVTVVT"
     gene            complement(2368..>2692)
                     /locus_tag="VNP73_04755"
     CDS             complement(2368..>2692)
                     /locus_tag="VNP73_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWL65265.1"
                     /translation="AHPRIAHMDLAYHDVNRKRGIYYRMEARGMAERMCDELDIEKAT
                     QEPPQTTRAKLRGEFIRAAKRKRRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVAK
                     LIASL"
BASE COUNT          464 a          936 c          807 g          485 t
ORIGIN      
        1 cccccgcata gagaacgagg gcttccgcgg gggcgagtcg caggggggcc gcgaagtagt
       61 cggccatccg cacgtagacg cgatcctcgt ccagatcgac atcgatcaga tcccccggcc
      121 cgtatccggg cagaccgcag aggaagacga ggttcagatc atcgagtagg tcgcgcttat
      181 cgatcccgaa tttgccggca agctcgccga ccgtcacacc ggggtgttgg atcgcgtatg
      241 ggaggagcag aagtatccgg ctcaagcggc ggccgagtcg gcccgacggg tcttcaggca
      301 tcggctcgga cggcgaacgg agccacgtgg tccacgagct tcttacgtag ttcggccggc
      361 gacacgatct gaacgcgtcc accgaatccg atcacccaag agatcagcgc atcggcgttc
      421 gcgacgctca gctcgatgtc caacgaaccg tccggcccgg ccgtcttcgg ctctgccatg
      481 ttctgttcgg cccaccagcg gaagtcgggt ccgaacctga cccgcgccgg caccggttcg
      541 ggccctacct cccacgcttc gaggtccaag tgatcggctg gacggaagtc ttcggggggc
      601 gagaaggtcc gcccgaggcg ctcgaccgtt ccaccgagcc gggacacttt gaagccacgg
      661 atcgcgtctt tcgaggtgtc gcgtccgacc acgtaccagt tccctcgtcg atgaaccagc
      721 ccgtagggct ccagctcgcg catgaccacc gttccgtcgg tgcctgcatg ctcgaaggag
      781 atggcctccc tctccgatac ggccgcgtac aaagggccca gtaggggttg ctcggcggct
      841 aagtccgtgc cccacacgac ccgcggaccg ttccagcctt cctgcggcag atcgagggac
      901 aacttcatca tcccgctggc cgcttgcggg ccggctccga gcaccgcgcc ggccgcgatg
      961 ctaagagccg cgagctcgtc cggttccaga tccaactcgg gcaagtagta cttctctttg
     1021 gggatcacat atccgtcacc gtcgtccccc agcggatcgg tcgttaccaa ctcgagaggg
     1081 atccccatcg aacgaagtgc ctccttgtcc cgttcgaagg cgcgcctgaa tgcttctcga
     1141 ttgtcctggt cgtatccggc gatctggcga cggatgtctt cggccgtcaa gggtcgctgt
     1201 gtctccagca aggctgctat caggttgatg agccgttcga tcctgcgcac atagcgaggg
     1261 taggccatgg ccgcgagcaa aaccgctccc ggccgtccct tatgtgacta ccgtcaccct
     1321 ctgagccgcg atccggccag ccgccgaggc cgccaggaag ggctcgggcg attcgctcat
     1381 cgagcggccc atcgtcgacg gatcgatccc gcgctcacgg aggagatcga gcccgggacg
     1441 cccgtcggcg atcacgacgt cctggctcgc gacggtcttc aactgggtac gcacaagagc
     1501 cccccgctct tcgggaagct ccgggagaac gatcgtcacc ctgcgggcca caaggccgat
     1561 cgacgtgatc gagtgatggc tgatcccctg atgccgatct cgtgcatcaa cgaacgagat
     1621 gcgcagagcc gcgatcgcgc tcccccccaa ggcggccgcc gcgtcgagga cctgcgcttg
     1681 ctcgatgccc gagtagccaa gtcgcgttcc agtccccact ccaccgggcc ccatcgctac
     1741 aacgatcgcg tctgcacggc ccaccatctt tgtcgcgacc agtgcgctgt aaacattcac
     1801 agcctcgagg tcccctccaa acgcatgccc gtacgtacac gtgagatcga tcactccgga
     1861 ctcgcggagt tcgcgcacca ggttgctcca cgcgataggc agcgatcccg catcgctcat
     1921 gacgtacgca acccgcgttt ccggtcgctc cgccttgatg ccggctgcga ccgcggctat
     1981 ctgggaatgg agtccgcacg cgactaccgg cgtgccgtcg agatcatccg catccacgag
     2041 cgacatgtgg tgctcgctct ccggcgcctc ggccgtcagg acgttcgtct gccacggcgt
     2101 gtagcgcatc ttcacgatgt ggccttcgcc gggttctatc tcggggccgt cgagattcca
     2161 caggacgaaa ccctcgccgc ccgttcccaa gccgagctcg agcccggtgg tgttcacgac
     2221 cactcggtcc cccacgttca actgccctac catcgccgga taaccgctgg ccgggacctc
     2281 gtcggcaccg atcgcgacgc gcactttgac gagacccggt ccctcggact cgatcgacac
     2341 gaccttgccg gcgcggaaag cggccatcta gagcgatgcg atcaacttcg cgactcgctc
     2401 gtcgaccgat cggaacgggt ccttgcacag cacggttcgt tgagcttgat cgttgagctt
     2461 gaggtggacc cagtccaccg tgaagtcgcg acgtttacgc ttcgcggccc ggatgaactc
     2521 cccgcgcagc ttcgcgcgag tcgtctgcgg tggctcttgg gtcgccttct cgatatcgag
     2581 ctcgtcgcac atacgctccg ccataccccg agcctccatc cggtaataga tccctcgctt
     2641 ccgattcacg tcgtgatagg cgaggtccat gtgagcgatc cgtgggtgag cc
//
LOCUS       DATMED010000188         4657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_149659, whole genome shotgun sequence.
ACCESSION   DATMED010000188 DATMED010000000
VERSION     DATMED010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149659"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VNP73_04760"
     CDS             complement(<1..1089)
                     /locus_tag="VNP73_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65266.1"
                     /translation="MLEAAFARIGGRNRISIGLLLLAFIVTRFLGASGAVGFGARTDV
                     EIAYRHWAVTVVERDQVPYAQVNVEYPPGSLPFVLAPQLTTDTEHSYRVLFVSLMVLV
                     DALGLLGLMVLSRRNGSMLGPWMWVVGLWILGPFIFLRLDLVPAVATIWAMERATNGK
                     WGAGGALLGFGAIAKLYPLIILPVLFIMAPREAKKRVLIGAGAVAAVFLAPFLLILPD
                     VFNEVIGYHSKRGIQIESLWGSLLYVARSGTPIKFILHNYGAHHFEGGSTSMLKAIAG
                     LATIGAVSLGAWVAHTSKRLPENLAGICFATLTLTVAVASVLSPQFFVWVVALAAVAL
                     CWPKSPVQLQAAALLFILLLTRLIYPMLH"
     gene            1175..2818
                     /locus_tag="VNP73_04765"
     CDS             1175..2818
                     /locus_tag="VNP73_04765"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWL65267.1"
                     /translation="MTDAAPGVRDGTSAPAEPARSQVTYSYEGPDMAGAASELVEKKD
                     KFRKGGGPERVQRQKDQGKLFVRERIDLLFDEGTFFEHGLLAHHQSQSPAMQGKFTPA
                     DGVITGIGEIDGRKTAVIAYDFTVMAGSMGMVTEIKSSRMRELALRERIPLVWLIDSA
                     GARIQEATGSMFARTGDLFREQVTMSGVIPQVAAMMGPGAAGTAYIPGLADFVPMVKG
                     TSNMALAGPHLVKAAVGEDVTAEEMGGSKVHCKVSGVGDLEVEDDVSCIEAVRSYLSF
                     FPSSNTDPLPKRDVSDPIDRRAEELHEIVPANPRQAYDVYKVIKAITDDGEFFPMKPD
                     WARNLVTGFARFGGRPSGIVANQPKFLGGALDVNSADKAARFVWLCDAFNIPLVFLMD
                     CPGFLVGSNVEKQGIIRHGAKMLFAVAEATVPKVTVILRKGYGAGYYVMNGRAYEPDL
                     IVGWPTAEISVMGPEGAVNIIFRKQIEAMPEDEQEKARNEMVAAIREQISPYIAAGWS
                     FIDDLIDPADTRQTIIRGLQSAANKTVERPWRKHGVLPV"
     gene            2859..3815
                     /locus_tag="VNP73_04770"
     CDS             2859..3815
                     /locus_tag="VNP73_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HWL65268.1"
                     /translation="MRLRFLGLAAGTVVAIGVAGYMGIERWSFLDSLYMTLTTLTTVG
                     FGEVHPMDPSGRIFTIVLLVMGVGLIAVLLALLAQLVQEGALGERGRRRRMQKRIEDM
                     KDHYIVCAYGRVGRTVAREFEAEGIDFVVIDKDGELEDEMLRDGVTFLIDDPSRESVL
                     YSAGVERAKGVVSAVDDDAQNLYITLTARALKPDIYIVARAAEEATAARLYTGGADRV
                     ISPYVSSGRHMALLALRPQMVDYLEVESEKAGALRLEELLVEEGSELVDKALADTLEE
                     GSCLVLRRVSGDTFTNPDSATKIEAGDLLILLADPVVKEGAR"
     gene            3812..>4657
                     /locus_tag="VNP73_04775"
     CDS             3812..>4657
                     /locus_tag="VNP73_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL65269.1"
                     /translation="MTRDGYALANGIRMFYVEEGEGPLVLLCHGFPESWYSWRHQLAP
                     IGEAGFRAVAVDLPGYGRSDKPDATYDVVWLTDCLAGLIEALGHETAVVVGHDWGGLL
                     AWPFARLHPERTAGVIGLNVPDLPRPPVPTTEWLAERGGDQHSYMLFFQKRYEAEAVI
                     EANIDQFVTGIFLGPVTVQKEAFTKDDLKVYADTFRPRGSITPPLEYYRNMDRNWELM
                     APHDDTKIEVPCLMICAEGDTVLPPRLAEGMDSRVPDLEVVTVADCGHWTQQEQPQVT
                     THHVLA"
BASE COUNT          926 a         1400 c         1514 g          817 t
ORIGIN      
        1 gtgcagcatc ggatagatca ggcgcgttag cagcaggatg aacagcagag ccgctgcttg
       61 aagttggacc ggcgacttcg gccagcacaa cgccaccgca gcgagcgcga cgacccaaac
      121 gaagaactga ggcgaaagca cgctcgcgac cgcgaccgtg agggtgaggg tggcgaaaca
      181 gatcccggcc aggttctccg gcaacctctt gctggtgtgt gccacccacg cgcccagaga
      241 gacggcaccg atcgtggcga gaccggctat cgctttcaac atcgaggtcg acccaccctc
      301 gaaatgatgc gcgccgtagt tgtggaggat gaacttgatc ggggttccac tgcgagccac
      361 gtaaagcagt gatccccaca ggctttcgat ctggatacct cgtttggagt ggtatccgat
      421 cacctcgtta aagacatcgg gcaatatcaa caggaacggc gcgaggaaca ccgccgcgac
      481 cgctccggct ccgatcagga cgcgcttctt cgcttcgcgt ggagccatga tgaacagcac
      541 gggaaggatg atcaatggat agagcttcgc gatcgcccca aagcccagga gcgcgcctcc
      601 cgccccccac ttgccgttgg tcgctcgttc catcgcccag atcgtcgcta cggccgggac
      661 taggtcgagc ctgaggaata tgaacggccc gaggatccac aggcccacga cccacatcca
      721 tggacccagc atcgatccgt tccggcgcga gagaaccatg agccccagaa gcccgagtgc
      781 gtcgaccagg accatcaacg aaacgaacag gacacggtag gagtgctccg tgtcggtcgt
      841 gagctgaggc gcgaggacga atgggagaga accgggtggg tactcgacgt tcacctgggc
      901 gtatgggacc tgatctcgct ccaccacggt gaccgcccag tgccggtagg cgatctcgac
      961 atcggttcta gccccgaacc cgaccgcgcc cgacgccccc aggaacctgg tgacgatgaa
     1021 ggcgagcagc aacagtccta tcgagatcct gttgcggccg ccgatccggg cgaacgcggc
     1081 ttcgagcatg gtcggtaatc ttccctcagc gaggcccggg aaggcatcgg agcatcaggc
     1141 tccggcgaag ggccccgaaa agaggtgata gccgatgacc gacgccgctc ccggcgtgcg
     1201 ggatggcact tccgcccctg ccgagcccgc ccgatctcag gtcacgtact cgtacgaagg
     1261 tccggacatg gccggcgccg catccgagct ggtcgagaag aaagacaagt tccggaaggg
     1321 cgggggcccc gaacgggtcc aacgacagaa ggaccaaggc aagctcttcg tcagagagcg
     1381 gatcgatctt ctcttcgacg aaggcacctt cttcgagcac ggtctcctcg cacatcacca
     1441 gagtcagtcg ccggcgatgc aagggaagtt caccccggcc gacggcgtga tcaccggtat
     1501 cggcgagatc gatgggcgaa agaccgcggt gatcgcatac gacttcaccg ttatggccgg
     1561 ttccatgggg atggtgaccg agatcaagtc ttctcggatg cgcgaactcg cgctacgaga
     1621 acgcatccca ctcgtgtggc ttatcgactc cgccggagct cggatccagg aagcgaccgg
     1681 atcgatgttc gctcgcaccg gggacctgtt ccgcgagcag gtcacgatga gtggcgtcat
     1741 cccccaggtc gcggcgatga tgggaccggg agccgccgga acggcctata tcccgggtct
     1801 cgcagacttc gttccgatgg tgaaaggcac ctcgaacatg gcccttgccg gcccgcacct
     1861 ggtgaaggcc gcggtcggtg aggacgtcac cgcggaggaa atgggtggat ccaaggtcca
     1921 ctgcaaggta tcgggtgtgg gcgacctcga ggtggaagac gacgtctcat gcatcgaggc
     1981 ggttcgtagc tacctttcat tctttccttc gtccaatacc gatccgctgc cgaaacgcga
     2041 cgtgtcggat ccgatcgaca gaagggcaga agagttacac gagatcgttc cggcgaaccc
     2101 gcgccaggcc tacgacgtct acaaggtgat caaggcgatc accgacgacg gtgaattctt
     2161 tccgatgaag cccgactggg cgcggaacct cgtcactggg ttcgcgcggt tcggcggccg
     2221 tccgtccggc atcgttgcga accagccgaa gttccttggg ggcgcgctcg acgtgaacag
     2281 cgcggacaag gcggctcgat tcgtgtggct ctgcgatgcg ttcaacatcc cgctcgtgtt
     2341 tctgatggac tgccccggat ttctggtcgg cagcaacgtc gagaagcaag ggatcatcag
     2401 gcacggcgca aagatgctgt tcgccgtcgc cgaagcaacg gttcccaaag tgacggtcat
     2461 attgcggaag ggatatggcg ccggctacta cgtgatgaac ggtcgggcat acgagccgga
     2521 tctgatcgtc ggatggccca cggcggagat ctcggtcatg gggcccgaag gagcggtgaa
     2581 catcatcttc cgcaaacaga tcgaggcgat gccggaggac gagcaggaga aggcccgaaa
     2641 cgagatggtc gcggcgatcc gcgagcagat ctcgccctac atcgcggccg gctggtcgtt
     2701 cattgacgac ctgatcgacc cggcggatac gcggcagacg atcatccgag gcctgcaatc
     2761 ggccgcgaat aagacggtcg agcgaccgtg gcgaaagcac ggggtgctgc ccgtttagtg
     2821 ggctaggcat gcctcaatca agaacggacg atccatgggt gcgactccgg tttttggggc
     2881 ttgccgccgg cacggtcgtt gccatcggag ttgccggtta catgggcatc gagcgctggt
     2941 cgttcctcga ctcgctctac atgacactca ccactctgac cactgtcggt ttcggggagg
     3001 tccatccgat ggatccgtcc gggcggatct tcacgatcgt cctactggtg atgggcgtcg
     3061 gtctcatcgc cgtgctgctg gctttgctcg cacagttggt tcaagaggga gcgctggggg
     3121 agcgtggtag gaggagacgc atgcagaagc gcatcgagga catgaaggat cactacatcg
     3181 tctgcgctta cggacgggtg gggcgcaccg tagctcgtga gtttgaggcg gaggggatcg
     3241 acttcgtggt gatcgataaa gacggcgaac tcgaagacga gatgttgcgg gatggagtga
     3301 cattcttgat cgacgaccct tcacgcgagt cggtgcttta ttcagcgggt gtcgagcgag
     3361 ccaaaggggt cgtctccgcg gtagacgacg acgcgcagaa cctctacatc acgctcaccg
     3421 cccgggcact caagccggac atctacatag tggcccgtgc cgcggaagaa gcgacggccg
     3481 cacggctata caccgggggc gcggatcgcg ttatctcgcc gtacgtgagt agcggacggc
     3541 acatggcgct gctcgctttg cgtcctcaga tggtcgacta cctcgaggtt gaatccgaga
     3601 aagccggtgc tctgcgattg gaggaactcc ttgtcgagga ggggtccgaa ctggtcgaca
     3661 aggcgctggc cgacaccctc gaggagggtt cgtgcttggt attgagacgc gtgagtggag
     3721 acacgttcac gaatcctgac tccgccacga agatcgaagc cggtgacctt ctcatcctgc
     3781 ttgccgaccc ggtagtgaag gaaggagcgc gatgacccga gatgggtacg cgctcgccaa
     3841 tgggatccgg atgttctacg tcgaggaggg cgagggacca ctcgtgcttc tctgtcacgg
     3901 cttccccgag tcctggtact cgtggcgcca tcaactggcc cccataggtg aggccggctt
     3961 cagagccgtc gcggtggatc tccccggtta tggccgctcc gacaagcccg atgccaccta
     4021 tgacgtcgtg tggttgacgg attgcctggc gggcctgatc gaggctctcg gccacgagac
     4081 ggccgtcgtg gtcggtcatg actggggagg gctcctggcg tggccgttcg cccgtctgca
     4141 ccccgagagg accgctgggg tgatcggact caacgtcccc gacctgccca ggcccccggt
     4201 ccccacgacc gaatggctgg cggagcgggg aggcgaccag cactcctaca tgttgttctt
     4261 ccagaaacgc tatgaggccg aggccgtgat cgaggcgaac atcgaccagt tcgtcaccgg
     4321 gatcttcttg ggtccggtga ccgtgcagaa ggaagctttc accaaggacg acttgaaggt
     4381 ctatgccgac acgttccgtc cccgaggttc gatcacgccc cctctcgagt actaccggaa
     4441 catggatcgg aactgggagc tgatggctcc ccacgacgac accaagatcg aggtcccctg
     4501 cctcatgatc tgcgccgagg gggatacggt gctccctccg cgtcttgcgg aggggatgga
     4561 ctcccgtgtg cccgacctgg aggttgtgac cgtggccgac tgcggacact ggacccagca
     4621 ggagcagccg caggtcacta cccaccacgt gctggct
//
LOCUS       DATMED010000189         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3217509, whole genome shotgun sequence.
ACCESSION   DATMED010000189 DATMED010000000
VERSION     DATMED010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217509"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /gene="aroE"
                     /locus_tag="VNP73_04780"
     CDS             <1..280
                     /gene="aroE"
                     /locus_tag="VNP73_04780"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWL65270.1"
                     /translation="SFDVIVNATPLGSSGEDALGNAALHPSQVVFDLVYEPPTTPLVE
                     RAHAAGAGAWGGLGMLVHQAAASFRIWTGQEPPIEIMSAAGLHAVLRR"
     gene            360..845
                     /locus_tag="VNP73_04785"
     CDS             360..845
                     /locus_tag="VNP73_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HWL65271.1"
                     /translation="MTKADRLSAALDEFLTASVDIEAAAIVSIDGLPIASALPVDVAE
                     DRLAAMSAALLSLGEKAAEGLGRGELAQVFVEGENGYVVLMAAGPQAVLVSVTSRQAK
                     AGLVLFEMRRACERIKKVLADTDRPAETTQPSAEAPSAQAPIGQTPDMEALVPPVPSW
                     Q"
     gene            868..>2177
                     /locus_tag="VNP73_04790"
     CDS             868..>2177
                     /locus_tag="VNP73_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HWL65272.1"
                     /translation="MLSGTLDDFTLPDVFRILSSCDKTGRLHIARSAGEGTVHFRDGD
                     VYLAESSLTRDPLGQKLLRARLVSEVQLRQALDEQAESGKRLGEVLVESGVLGHEQIE
                     ATVRQQIEDAVFDLLRWELGEFSWEPGLEVDAEVSISVSVENLIIEASRKLDELEVIT
                     KKIPSEEAILTMAPSPPEDAAEINITPDEWRVLVLVNGTRSVVAIAEAVGLDNFSTMR
                     VLYGLASSGLIEVSDTQASEALEEFEAAVAAEPPVQADEPAVEVEEPLVEEISEPLVA
                     EVGSESELEAPAETVETAEVASEVDAIDQEATEEQDERTPEEVFADELLTDPVDFVPD
                     ESEGDGSGSNGSSESIPVTAGPDDEEVPVVDRLAAVKELADLFDPPDPEAAGPPYPPE
                     THFAEEAAAAAAAAAEPPVFHRMEDDEEITKGLLSRLIDGVKGL"
BASE COUNT          398 a          690 c          705 g          384 t
ORIGIN      
        1 atcgttcgac gtcatcgtca atgcgacccc tctgggttcg tccggcgagg acgcgttggg
       61 gaacgctgca cttcatccct ctcaagttgt tttcgatctt gtctatgagc ccccgacgac
      121 acctcttgtc gagcgggccc acgccgcggg cgccggcgcg tggggcggac ttgggatgct
      181 cgtgcatcaa gcagccgcgt cgttccggat ctggactggg caagaaccac ccatcgagat
      241 catgtccgcc gcgggccttc atgccgtttt gaggagatag cccgctcccc tcaacttcgt
      301 cactgcagct gccgaagagt taatcgactg cccctgcccc cccgctctag gaggcaccca
      361 tgaccaaggc cgatcgactt tcggctgccc tggacgaatt cctgaccgcc tctgtggata
      421 tcgaagccgc agcgatcgtc agtatcgatg gtctacccat cgcgtccgca ctacccgtcg
      481 atgtggccga agatcggctc gcagcgatga gcgccgcact tctttctctc ggtgagaaag
      541 ctgccgaagg cctcgggcga ggtgagctcg cacaggtctt cgttgaaggt gagaacggtt
      601 acgtcgtgtt gatggccgcc ggcccccaag cggtcctcgt ctcggtcaca tcccggcaag
      661 cgaaggccgg tctcgtgctg tttgagatgc gacgcgcctg cgagcgcata aagaaggtcc
      721 tcgcggacac cgatcgaccg gctgaaacga cccagccgag tgccgaagcg cccagcgcgc
      781 aggcgccgat cggacagacg ccggatatgg aagcgctcgt tccgccggtt ccttcttggc
      841 aataactaag gtaaagggag gctcgaagtg ctaagcggca cgctggacga tttcaccctt
      901 ccggatgttt tccggatcct ctcttcgtgc gacaagacgg gtcggctaca catcgcccgg
      961 tcggccggag agggcacagt gcacttccgc gacggcgacg tatatctcgc cgaatcaagt
     1021 ctcacccggg atccattggg ccagaagctc ctccgtgcgc gactcgtgag cgaggtccag
     1081 ctgcgccagg ccctggacga gcaagccgag tccggcaagc gcctgggcga ggtcctcgtc
     1141 gagtccgggg ttctcggtca cgagcagatc gaagcgaccg ttcgccagca gatcgaggac
     1201 gccgtcttcg accttctccg ctgggaactc ggcgagttca gttgggagcc cggtctggag
     1261 gtcgacgccg aggtttcgat ctcggtcagc gtcgagaact tgatcatcga ggcgtcgcgc
     1321 aagctcgacg agctcgaggt gatcaccaag aagatcccgt ccgaagaagc gatcctcacg
     1381 atggcaccgt cgcctccgga ggacgccgcc gagatcaaca tcactcccga tgagtggagg
     1441 gtgctcgtcc tggtgaacgg aactcgctcg gtggttgcga tcgccgaggc ggtcggcctc
     1501 gacaacttct cgacgatgag ggtcctctac gggctcgctt cctcggggct tatcgaggtc
     1561 tcggacacac aggcctcgga agcgttggaa gagttcgagg ccgccgtggc cgccgagcct
     1621 cccgtgcagg ccgacgagcc tgccgtcgag gtcgaagagc cgctggtgga ggagatctcc
     1681 gagccgctcg tggcagaggt cggatccgag tcggagctcg aggcacccgc cgagacggtc
     1741 gagaccgcgg aggtcgcgag cgaggttgac gcgatcgatc aagaagcgac cgaggagcag
     1801 gatgagcgga cgcccgaaga ggtcttcgcc gatgagctgc tgacagatcc ggtcgatttc
     1861 gtcccggacg agtccgaagg cgatgggtcc ggttccaacg gttcgtccga gagtatcccc
     1921 gtgaccgcgg gtccggacga cgaggaagtc ccggtcgtgg accgactcgc cgccgtgaag
     1981 gaactcgccg acctgttcga cccgccggac ccagaggcgg ccggcccgcc gtatcccccc
     2041 gagacgcact ttgctgaaga ggccgctgcg gcagccgcag cggcggcaga acctcccgtc
     2101 ttccaccgta tggaagacga cgaagagatc accaaaggtc tgttgtcccg attgatcgac
     2161 ggggtcaagg ggctcta
//
LOCUS       DATMED010000190         3332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4363029, whole genome shotgun sequence.
ACCESSION   DATMED010000190 DATMED010000000
VERSION     DATMED010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4363029"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..656
                     /locus_tag="VNP73_04795"
     CDS             138..656
                     /locus_tag="VNP73_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWL65273.1"
                     /translation="MTIELAYWIALLVGVTFLLLSVVLGDVFSFLDFLDFDFGDGFSA
                     TPVLFTTLAAFGGGGLLAINAFGASPGPSVLWGLGTGVIGGAFSAGLFWLLHRQEAGE
                     GFMVSQLVGERGRCTLAIHPGKEGRVSVQYEGMTRSFTATSDDEIAAGVDVVVKDVIG
                     TSLKVARSEGVG"
     gene            680..2068
                     /locus_tag="VNP73_04800"
     CDS             680..2068
                     /locus_tag="VNP73_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWL65274.1"
                     /translation="MPEVPAVVAALIVVGVALLAFAASRYKVAKPNEALIIAGSRRSR
                     GGIRVVVGGGAIIYPLVNVAKRISLEIQSIPMALTGAVTTQGVPVSVEGIVNVKISDD
                     EDSIRQAAQRFLDSQAKVPEIVRNVMEGSLRTIIGQLTVSELIRDREVFAARVQEVAN
                     GDLQGTGLAIDVFTIQSISDTEEYIENLGRKAAAEVRRDAEIAEASAEREAREQQALA
                     KQAIFEAEKATSLKEQGVLQEVAGAQARAEQAKPLADAQARRDVVLQETEVAELVAQR
                     RDKELDAEIRRTADAQRYAAEQTAEARRYTVKAEAEAARDRRLLEAEAERANLEAVAS
                     GRSFEERELGKAKAEATLSVGEAEAKAMAKKADAYKQYEEAATLELLVSRLPEVARAI
                     AEPISNVKEITIIDTEGANKLTRVTANTVRQVDAVVESFTGASLSTLVGRYLKGGNGA
                     ETGDGKTPEEGT"
     gene            2084..2800
                     /locus_tag="VNP73_04805"
     CDS             2084..2800
                     /locus_tag="VNP73_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65275.1"
                     /translation="MTVPLDLAGPVFEGWYWGVFFLAIFVGAIALFAGVVTAIVAAIA
                     RARGKPLGRTKAIVLLVCLTLVLSSGILIWRRLSSEMEFDQTNRRAAAAFDFEPYLPA
                     DLPSGWSSSRRQAISTDGGELWVTYEHPEGYIFTGQHSSIPGFRFDPPEHCSIGVRGL
                     SSGSFDGPCRRLTTPRGLDVYVGGDAHTAGDTMAIAVLQNTTVRLAYFETYVADEDLV
                     LYFDSLRPVDPLSIDYFDRG"
BASE COUNT          641 a          985 c         1103 g          603 t
ORIGIN      
        1 cggggtcgag cttccggagc agttcggcga tcaggcccac cttggccgag cgagagctcg
       61 tctcgctgag actgatagag caggcgacga ggtcggcgaa cagcatcatc cttagccggc
      121 gacgatagcc ttcccccatg acgatcgagc tggcctactg gatagccctg ctggtcggcg
      181 tgactttcct cttgctctcc gtcgttctgg gcgacgtctt cagtttcctc gacttcctcg
      241 atttcgattt cggcgacggc ttctccgcaa cacccgttct gttcacgacg ctggccgcgt
      301 tcggaggcgg cggcttgctc gcgatcaacg cgttcggagc gagcccgggg ccgtcggtgc
      361 tgtggggcct cgggacgggt gttatcggcg gcgccttctc cgccggactc ttctggctac
      421 tccatcgcca ggaagcgggc gagggattca tggtcagtca acttgtcggc gagaggggac
      481 gttgcacgct tgcgatccat cccggcaaag agggccgggt ctcggtccaa tacgaaggga
      541 tgactcgctc tttcaccgct acgagcgacg acgagatcgc cgccggcgtg gacgtcgtcg
      601 tgaaagacgt gatcggtaca tcgttgaaag tcgcacgtag tgagggagtg ggataggacc
      661 accaagaccg gagggtgtca tgcctgaagt tcctgcagta gtagccgctc taatcgtcgt
      721 cggcgtcgcg ctgctcgcgt tcgcggccag tcgctacaag gtcgcgaagc ccaacgaagc
      781 gttgatcatc gccgggtcgc gacgatcgag aggcggtatc cgcgtcgtcg tgggtggtgg
      841 cgccatcatc tatccgctcg tcaacgtggc gaaacgcatc tcgctcgaga tccagtcgat
      901 cccaatggcc ttgaccggag ccgtcactac gcagggtgtg ccggtctccg tggaaggcat
      961 cgtgaacgtc aagatctccg atgacgagga ctccatccgc caggccgcgc aacggttcct
     1021 ggatagccag gccaaagtgc cggagatcgt gcgcaacgtc atggaaggtt ctctaagaac
     1081 catcatcggt cagctcaccg tcagtgagct catccgcgac cgggaggtgt tcgccgcgcg
     1141 cgtgcaggag gtggcgaacg gtgacctcca gggaacggga ctggcgatcg acgtcttcac
     1201 gatccagtcc atctcagaca ccgaggaata catcgagaac ctgggtcgca aggccgccgc
     1261 ggaagtgcgt cgcgacgcgg agatcgccga agcctcagcg gagagggaag ctcgcgagca
     1321 gcaggcgctg gcgaagcagg cgatcttcga ggccgagaag gcgacgagcc tcaaggaaca
     1381 aggtgttctt caggaggttg ccggggccca agcgcgcgca gagcaagcga aaccccttgc
     1441 cgacgcccag gcgagacggg atgtcgtact ccaggagacc gaggtcgcgg aactcgtcgc
     1501 gcaacgccgt gacaaggagc tcgatgccga gatcagacgc acggcagatg cccagaggta
     1561 cgccgccgaa cagacggcag aagcgcggcg ctacacggtc aaggcggagg cggaagcggc
     1621 gcgggatcgc aggcttctcg aggccgaggc cgaacgcgcc aatctcgagg cggtcgcctc
     1681 tggacggtct ttcgaagaga gagagctggg caaggcgaag gccgaagcga cgctgtccgt
     1741 cggcgaggcc gaagccaagg cgatggcaaa gaaggcggat gcctacaagc agtacgaaga
     1801 ggcggcgacg ttggaactcc tggtgtcgcg gctccctgag gttgcgcggg cgatcgccga
     1861 accgattagc aacgtcaagg agatcacaat catcgacaca gagggtgcca acaagctcac
     1921 ccgtgttacc gccaacaccg tgcggcaagt agacgccgtt gtggaatcgt tcaccggagc
     1981 gagcctgagc acgctggtgg gccgttacct gaagggcggg aacggagccg aaaccgggga
     2041 cggcaagacc cccgaagaag ggacttaacc agcccactgg tcagtgacgg tgccgctcga
     2101 cctcgccggc cccgtcttcg agggctggta ttggggcgtt ttcttcctcg ccattttcgt
     2161 cggggcaatt gctctgttcg ctggggtggt cactgccatc gtggctgcga tcgcacgtgc
     2221 acgaggcaag ccgctcggac gaacaaaggc gatcgttctt ctggtgtgcc tcaccttggt
     2281 gctctcgagc gggatcttga tctggcgccg gttgtcttcg gagatggaat tcgaccagac
     2341 caaccggcgc gcggcggcag cttttgattt cgagccttat ctacctgcgg atctcccatc
     2401 aggatggtcg tcatccagga ggcaagccat ctcgacggat ggtggcgaac tgtgggttac
     2461 ctacgagcat ccggagggat acatcttcac gggacaacac agctcgatcc ccggattcag
     2521 gttcgatccg ccggagcact gttcgatcgg ggtccgtggt ctcagttccg ggagtttcga
     2581 cggtccttgc aggcgcctga cgacaccccg tggattggat gtttacgtcg gcggagacgc
     2641 acacaccgcc ggagacacga tggcgatcgc ggtcctacag aacacgacgg tcaggctcgc
     2701 ctacttcgag acctacgtgg cagatgaaga cctggtcctc tacttcgatt cacttaggcc
     2761 cgtcgatccg ttgtcgatcg actatttcga ccggggctga ctccgatcac aaggtgcgcg
     2821 gtaagacagc ttgcccctgt agcggctgcc agttcgggtg gagatccacc ctgcgggatc
     2881 gcgtgatcgg atggggattg ctcccgtctc ggttcatgac ccagatcgca tcgctgtcgc
     2941 ggcatggggt gcccggtgtg gtcctccgac ggtcggaaca tcgcgttcgt cgggcaacac
     3001 ggaggatcaa ggaactccac cctgaggtga aggtcgtatt cctcagcgcg tacgccgacg
     3061 cgagtctttc agcaggaagc cgaggaggcc ggtggcgagg gatggctact caagggcgac
     3121 caccctcgcg acctctgtga caaggtgctc gctctgacgg ggcgtcccgg ctaggggcag
     3181 cttgcttttc cgactcccct tctaacaaaa agctcatcgt cctctcatag ctgcggggta
     3241 atactgggac aaagggcttg ctcggtacca acgcagggtc aggtggctga aaacgggggg
     3301 cgacatgagc cagctatccg ccggcgtgac ga
//
LOCUS       DATMED010000191         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2460692, whole genome shotgun sequence.
ACCESSION   DATMED010000191 DATMED010000000
VERSION     DATMED010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460692"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1566
                     /locus_tag="VNP73_04810"
     CDS             67..1566
                     /locus_tag="VNP73_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HWL65276.1"
                     /translation="MIKQDQLMWIDGREVAAHDGGTLEVENPATGQIAFVVARGQAPD
                     IDAAVDAARRAHDDGPWPDMHPRERGRILRAAAEILRSRKEAIGEVETTCVGRPLREM
                     VQQVGRAPDWLDFLASVAETHEDLVVPASGELMNYVRRVPLGVVGAITPWNHPLLITM
                     KKVAPALAAGNTMVVKPSEVAPVAPLELARALVEAGLPDGVINVVPGFGAEAGKALTE
                     HDRIDRIDLTGGTETGRHVAAAAGRNLIPVQAELGGKAPVVIFDDVPIDVAVAGATFA
                     SFVASGQSCIAGARLLVHERSVGAFVEKLVERSQLIRVGDPMQRDAQMGPVVSQRQLD
                     RILELVESARAEGASVLCGGERLRGDAFERGYFIAPTVITDVRQDMRIMREEVFGPVV
                     SVQPFADEDEAVALANDADFGLGAAVWTRDLTRAHVVAHRIRSGTVWINDHHRIDPAS
                     LWGGFTDSGIGSENGVEAFNDYTAAQSVLVNLSRTPFDWFGSSDELRYS"
     gene            1566..>2311
                     /locus_tag="VNP73_04815"
     CDS             1566..>2311
                     /locus_tag="VNP73_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL65277.1"
                     /translation="MSGRQFILLTLNGISFGALLFLLSSGFTLIFGLMRIVNLAHGSF
                     YLLGGYVGLTLMQEGQSFWVAMIGGALAIAVLGFAVERGLLRRVRGRTMPEVLLTVGL
                     SLIFADLALARWGGDPILLDRPSQFANTVELGGVTYPEFRLLVLVLGVLLAAAMWILQ
                     SRTRIGAIIRAGVDDREMVSALGINVNAVFTGVFVFGAFLAGMTGVIGGVFLSLYPGA
                     DGEILNFALVVVILGGLGDLRGAIVGSLLVG"
BASE COUNT          383 a          709 c          765 g          454 t
ORIGIN      
        1 ccgcacccac tgggggacag gggtgcgtcg aagggggctc ctccccttcg aaagggggag
       61 tctggggtga tcaagcagga tcagctcatg tggatcgacg gccgcgaggt cgccgcccac
      121 gacggcggaa ctctcgaggt cgagaaccca gccaccggcc aaatcgcgtt cgttgtcgct
      181 cgaggtcagg cccccgatat cgatgccgcc gtcgatgccg cccggcgagc gcacgacgat
      241 ggtccctggc ctgacatgca ccctcgagag cgagggagaa ttctcagagc ggccgcagag
      301 atcctccggt cccgtaagga ggcgatcggc gaggtcgaga cgacctgcgt ggggcgccct
      361 cttcgagaga tggtccaaca ggttggccgg gcacccgact ggctcgactt cttagcctcg
      421 gtggccgaaa ctcacgaaga cctcgttgtt cccgcgagtg gcgagcttat gaactacgtg
      481 cgtcgcgtcc cactgggagt cgtcggcgca atcacgccgt ggaaccatcc actcctgatc
      541 accatgaaga aggtcgcacc cgcactcgca gccgggaaca ccatggtcgt gaagccatcg
      601 gaggtcgcgc ccgtcgcacc gctcgagctt gcccgagcac ttgtcgaggc gggccttccc
      661 gatggcgtaa tcaatgtcgt tcctgggttc ggagccgaag ccggcaaggc gctcaccgaa
      721 cacgaccgga tcgaccggat cgacctcacc ggcggcaccg agaccgggcg ccacgtcgcc
      781 gcggccgccg gaaggaacct catccccgtg caggccgaac taggcggcaa agcgcccgtc
      841 gtaatctttg acgacgtccc gatcgacgtg gcggtagccg gtgcgacctt cgccagcttc
      901 gtcgccagcg gtcagagctg catcgccggc gcccgtcttc tggtgcacga gcgctcggtc
      961 ggtgcatttg ttgagaagct cgttgagcgg agtcaactca tccgcgtcgg ggatccgatg
     1021 caacgggatg cacagatggg tccggtcgtt tcacaacggc aactcgaccg catcctcgag
     1081 ctcgtcgagt cggcgcgcgc cgagggcgcc agcgtgctgt gcggtggtga acgccttagg
     1141 ggggatgcct tcgagcgcgg ctacttcatc gcaccgactg tgatcaccga tgtccgccag
     1201 gacatgagaa tcatgcgcga ggaggtcttc ggcccggtcg tcagcgtgca gccgttcgcg
     1261 gacgaggacg aggccgttgc cctggcaaac gacgcagatt ttggactcgg cgcggcggtg
     1321 tggacgaggg acttgactcg cgctcacgta gtggctcatc ggatccgctc cggaaccgtg
     1381 tggattaacg atcaccatcg catcgatccg gcctcgctat ggggaggctt cacggattcc
     1441 gggatcggtt cggagaacgg tgttgaggcg ttcaacgact acacggcagc gcagagcgtg
     1501 ctcgtaaatc tcagtcgaac gccctttgac tggttcggct cgagcgacga gctgaggtac
     1561 agctagtgag cggacgccag ttcatcctgc tgacactcaa tgggatctcg ttcggagccc
     1621 tgctgttcct tctctcgagc ggcttcacct tgatctttgg gctcatgcgc atcgtgaacc
     1681 tggcccatgg atccttctat ctgctcggcg gctacgtcgg tctaacactg atgcaagagg
     1741 gtcagagttt ctgggttgcg atgatcggcg gcgcgcttgc catcgcggtg cttggattcg
     1801 cagtcgagcg cggcctgttg cgtcgcgtgc ggggccgcac tatgcctgag gtgctactga
     1861 cggttgggct ctcgctgatc tttgccgatc ttgcactcgc gcgatggggt ggtgacccca
     1921 ttctgctcga ccgaccttcg cagttcgcga acaccgtgga actgggcggc gtcacctatc
     1981 ccgagtttcg tctgctggtc ctcgttctcg gagtcctgct tgccgcggcc atgtggatcc
     2041 tgcagtcgcg tacgaggatc ggagccatca tccgggccgg tgtcgacgat cgcgagatgg
     2101 tctcggcgtt gggcatcaac gtaaatgcgg tcttcaccgg agtgttcgtg ttcggagcat
     2161 tccttgccgg catgaccggc gtcatcggcg gcgtctttct gagcctctat ccaggggccg
     2221 acggcgagat cctgaacttt gcgctcgtcg tggtgatcct gggcgggctc ggagatctgc
     2281 gcggggcaat tgtcggaagc ttgttggtag g
//
LOCUS       DATMED010000192         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2834584, whole genome shotgun sequence.
ACCESSION   DATMED010000192 DATMED010000000
VERSION     DATMED010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2834584"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VNP73_04820"
     CDS             complement(<1..200)
                     /locus_tag="VNP73_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65278.1"
                     /translation="MVNLFPYSPGSATELLVALHLPVAAWFAIAYPYMGGTIRSHERR
                     MDFVRFTGEWVIYYVLIALGGGV"
     gene            210..926
                     /locus_tag="VNP73_04825"
     CDS             210..926
                     /locus_tag="VNP73_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65279.1"
                     /translation="MDFVRFTGEFAIYYVLIGLGGGVLMGLTALILEPIQPDLGERVI
                     EWVMPMGVAGAAIVAAWLVESKQHVVENMAPVLTMLFTPLFAVMLTIAAVTYAVSGLA
                     GAFDRELLGVFDALMVVVLGLVLYAISARDPSRPAGMMDRVQLLAVASALLLDLMVLW
                     SMVARIGDLGFTPNRVAALGLNLVLLVNLARAAWLSLGFLRGQIAFHHLEHWQTSYLP
                     VFALWGATVVIIVPLFFGFE"
     gene            950..1201
                     /locus_tag="VNP73_04830"
     CDS             950..1201
                     /locus_tag="VNP73_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65280.1"
                     /translation="MNRIATIVGPALVGAFGVAAMIYGEADDAPGLVMFGLLITGGAL
                     AFGLKPSLRSRSRVMGFFVAAIAVTVVGSLVAGWLENTF"
     gene            1294..>2555
                     /locus_tag="VNP73_04835"
     CDS             1294..>2555
                     /locus_tag="VNP73_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65281.1"
                     /translation="MTRRAPWTTLLAILSLLTVLLPAGVVRAQVPEIPSSLNCDPLDP
                     KMCLFPFPNDFFTVPDASTDTGRRVNFGLDAMPRSGADVTNGGEGKPIDPAEWNRNDG
                     FSPGSMVMAYVPGIDLHTTWGTQGRPHSDAGPNEVGYFDYRDTIADPALSRAPDAPMV
                     ILNADTGERHPFWSELDTHPGAVEAGEQVLILRPAVNFDEGARYIVALRNLVNAGGET
                     IGAGEAFETYKGGSGADASRQAHYDTEIFPALEAAGVAKDDLYLAWDFTIASEENLAE
                     RMLTMRDDAFARILGDTNLADRQLGDSTAPDFVVDTVGERDDNYADAHGVAHTQRIRV
                     IDGRITVPNYMDRIQQTEGHVKANQLPADAPAPLSRLYDDPIDTDTLPDQNPVEPTVN
                     VPFRCEVAIAQDGQLLERENHPMLYGHG"
BASE COUNT          444 a          843 c          795 g          473 t
ORIGIN      
        1 acaccgccac cgagcgcgat gagcacgtag tagatgaccc actcgccggt gaagcggacg
       61 aagtccatgc gccgctcgtg tgatcggatc gttccgccca tgtacgggta ggcgatcgcg
      121 aaccacgccg cgacgggcag atggagcgca acgaggagct cggtcgccga gcccggactg
      181 tacgggaaga ggttgaccac gagcgcagga tggacttcgt tcgcttcacg ggggagttcg
      241 ccatctacta cgtgctgatc ggtctcggcg gcggcgtgct gatggggctc accgcgctga
      301 tcctcgagcc gatccaaccc gacctcggcg agcgcgtcat cgaatgggtg atgccgatgg
      361 gcgtagccgg tgctgcgatc gtggcagcgt ggttagtgga gtccaagcaa catgttgtcg
      421 agaacatggc gccggtgctc accatgctct tcactcccct gttcgccgtc atgctgacga
      481 tcgccgcggt tacctacgcc gtgtccgggc tcgcgggcgc atttgaccga gagctgcttg
      541 gtgttttcga tgcattgatg gtggtggtcc tgggactcgt gctctatgcg atctcggctc
      601 gggatccatc gcgaccggcc gggatgatgg atcgcgttca acttctcgcc gtcgcgagcg
      661 cgctccttct agacctgatg gtcctgtggt cgatggtggc ccggatcgga gatctggggt
      721 tcaccccgaa ccgcgtcgca gccctcggtc tcaatctcgt tctcctagtg aatctagcga
      781 gggccgcctg gctttcgttg ggattcctca ggggacagat cgctttccac cacctcgagc
      841 actggcagac gtcttacctg cccgtcttcg ctctgtgggg ggcgaccgtt gtgatcatcg
      901 tccctctgtt cttcggcttc gagtgacgcc cttaagtaaa ggagaactca tgaatcgcat
      961 cgccacaatc gttggtcccg ccctggtcgg tgccttcgga gttgccgcca tgatctatgg
     1021 agaagccgac gacgcgcccg gcctcgtgat gttcggcctc ctgatcaccg gcggagcgct
     1081 ggcgttcggc ctgaagccgt cccttcgtag caggtcacgc gttatgggtt tcttcgttgc
     1141 tgcgatcgcg gtaacggtcg tcgggagcct cgtcgccggt tggctcgaga acaccttcta
     1201 agtggagcgc cggacccccc gccgaggaag gagtttggcc cgtaacgtct aagaggtctt
     1261 ccaaccgatt ccccccatcg ctcaggagac ccaatgaccc gccgcgcccc ctggacaacc
     1321 cttctcgcca tcctttccct gctcaccgtt ctgctgccgg caggagtcgt gcgagctcag
     1381 gttccggaga tcccgtcgag cctcaattgc gatccgctcg accccaagat gtgtttgttc
     1441 ccgttcccca acgacttctt cacggttccc gacgcttcga ctgataccgg gcgcagggtg
     1501 aacttcggtc tcgacgcgat gccccgcagc ggcgcggacg tgacgaacgg cggcgagggc
     1561 aagccgatcg acccggccga gtggaaccgc aacgacgggt tctcaccggg ctcgatggtg
     1621 atggcctacg tccccggcat cgacctgcac acgacgtggg gtacgcaagg ccgtccgcac
     1681 tccgacgccg gcccgaacga ggtcggttat ttcgactatc gcgacacgat cgccgacccc
     1741 gcattgtcgc gggcaccgga tgctcccatg gtgatactga acgcagacac cggtgagcgc
     1801 cacccgttct ggtcggagct cgacacccac cccggcgcgg tcgaagccgg cgagcaggtg
     1861 ctcatcctcc gtccggccgt caacttcgat gagggagcgc gctacatcgt ggcgctgcgc
     1921 aacctcgtga acgcaggtgg cgagacgatc ggggcgggtg aagcgttcga gacctacaag
     1981 ggcggttccg gcgccgacgc gagccgccag gcccactacg acaccgagat cttccccgcg
     2041 ctcgaagcgg cgggagtcgc caaggatgac ctctatctcg cgtgggactt cacgatcgcg
     2101 agcgaggaga acctcgcgga gcgcatgctc acgatgcgcg acgacgcgtt cgctcgcatc
     2161 ctcggtgaca cgaacctcgc cgacaggcaa ctcggagaca gcacggcacc ggacttcgtc
     2221 gtcgacacag tcggcgagcg cgatgacaat tacgcggatg cgcacggcgt cgcgcacacg
     2281 cagcggatcc gtgtgatcga cggccggatc accgtgccga actacatgga tcgcatccag
     2341 cagaccgaag ggcacgtcaa agccaatcaa ttgccggccg acgcaccggc ccccctgtca
     2401 cgcctgtacg acgacccgat cgataccgac accctccccg accagaaccc ggtcgaacca
     2461 accgtgaacg tgccgttccg ctgcgaggtg gcgatcgctc aggacggtca acttctggaa
     2521 cgcgagaacc atccgatgct ttacggccac ggctt
//
LOCUS       DATMED010000193         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2460846, whole genome shotgun sequence.
ACCESSION   DATMED010000193 DATMED010000000
VERSION     DATMED010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460846"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VNP73_04840"
     CDS             <1..198
                     /locus_tag="VNP73_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65282.1"
                     /translation="SPGTALSPNTERLDVRCKTNVRRVDQIGMRAYIAQPFVQVDITL
                     SLNFRVTGRETGVDLGRCKSE"
     gene            250..1365
                     /gene="ftsZ"
                     /locus_tag="VNP73_04845"
     CDS             250..1365
                     /gene="ftsZ"
                     /locus_tag="VNP73_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWL65283.1"
                     /translation="MATGPQNYLAVIKVVGIGGGGVNAVNRMIEVGLKGVEFIAVNTD
                     AQALLMSDADVKLDIGRDLTRGLGAGADPEVGRQAAEEHRDEIEEVLKGSDMVFITAG
                     KGGGTGTGGAPIVAEIAKSIGALTIGVVTRPFTFEGRQRAVRAETGISNLKDKVDTLI
                     VIPNDRLLDVGDETTSVLEAFRMADEVLLQGVQGITDLITTPGLINLDFADVRAVMTD
                     AGSSLMGIGQARGDDRAQEAAKAAISSPLLEASIDGARGVLINVAGGSDLGMFEVNEA
                     ASIISKSAHPDANIIYGMVIDDTLGDEVRVTVIAAGFDRWGQLEADSLVKPDEEIFSL
                     PSIDESDSPAFLGDTERDRLVFDADEDLDIPSFLRNE"
     gene            1444..>2078
                     /locus_tag="VNP73_04850"
     CDS             1444..>2078
                     /locus_tag="VNP73_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HWL65284.1"
                     /translation="MKEMDLHPSLGHADVDGLCLLVDEAAREQGVIVAFSDRSGGVSK
                     VPFATLNLSTRAGDVKAAVIENRGRLMRSVGLTPESLAFLKQVHGAQVVDESAAHTGC
                     EGDVVVITKPGTTAGVLAADCVPVVVLGEDGLAAIHAGWRGLVGGAIEAGVARVGRPV
                     AAWVGPSIKGCCYEVGPDVVGAFHEKALPVADGRHVDTGAAAYALLMRTGV"
BASE COUNT          383 a          610 c          714 g          371 t
ORIGIN      
        1 tcgcccggaa cagcgctgag cccgaacacc gaacgtctcg acgttcggtg caaaacaaac
       61 gttcggcggg ttgaccaaat cggcatgcgt gcgtatattg ctcaaccatt cgttcaggtt
      121 gacataactc taagcctcaa ctttagggtg acgggcagag aaaccggcgt tgacctgggc
      181 agatgcaaaa gcgagtgatc gagcccttga agcattaagt cctactcgag ggtgtgaaag
      241 gaggttcacg tggcaaccgg accacagaat tacctggccg tgatcaaggt agttgggatc
      301 ggcggcggcg gcgttaacgc cgtgaatcgc atgatcgagg tcggcctgaa aggcgtcgaa
      361 ttcatcgcgg tgaataccga cgcgcaagcg ctgctcatgt cggacgcaga cgtgaagctc
      421 gacatcgggc gggacctgac acgagggctc ggcgccggcg cggatcccga ggtgggtcgc
      481 caggcggcgg aggagcatcg tgacgagatc gaagaggtgc tcaaaggctc cgacatggtc
      541 ttcatcaccg cgggcaaggg cggcggaacg ggcaccggtg gtgctccgat cgtcgccgag
      601 atagcgaagt cgatcggtgc tctgacgatc ggtgtcgtta cccgtccgtt caccttcgag
      661 ggacgccagc gtgcggtcag ggccgagacc ggcatctcga atctgaagga caaggtcgac
      721 acgctcatcg tgatccccaa cgacaggctg ctggacgtcg gcgacgagac cacctctgtt
      781 ctcgaagcat tcaggatggc cgacgaagtg ctccttcaag gagtgcaggg gatcaccgac
      841 ctgatcacca cgcccggctt gatcaacctc gacttcgccg atgtccgggc ggtcatgacg
      901 gatgcgggtt cttcgctcat ggggatcggt caggcgcgcg gtgacgacag ggcacaggaa
      961 gctgccaagg ctgcgatctc ctcgcctctg cttgaagcct cgatcgatgg ggcgagaggc
     1021 gtgttgatca acgtcgccgg aggctcggac ctcgggatgt tcgaggtcaa cgaggcggcc
     1081 agcatcatct ccaaatcggc gcaccccgac gcgaacatca tctacgggat ggtgatcgac
     1141 gacaccctcg gagacgaggt ccgtgtgacc gtcatagcgg ccggtttcga ccggtggggc
     1201 cagctcgagg ccgatagcct cgtgaagccc gacgaggaga tcttctcgct gccctcgatc
     1261 gacgagtcgg actcacctgc cttccttggt gacaccgaaa gagaccggct ggtcttcgat
     1321 gccgatgagg acctggatat cccgtccttc ctccgcaacg agtagtagta gtcgagccga
     1381 acttcctcgg ggcggggccg tttcggtccc gcccctctcg cgtccggacg gagaatcgct
     1441 ttgatgaaag agatggacct ccaccccagc ctgggccacg cggacgtcga cggcctctgc
     1501 ttgctcgtcg atgaagcggc acgcgagcag ggggtcatcg ttgctttcag cgaccgctcc
     1561 ggtggcgtga gcaaggtgcc gttcgcgact ctcaaccttt cgacgcgtgc gggcgacgtc
     1621 aaagccgcgg tgatcgagaa ccgcggtcga ttgatgcgtt cggtcgggct gacgccggaa
     1681 tcgctagcgt tcctcaaaca ggttcacgga gctcaggtgg tcgatgagag cgcggcgcac
     1741 accggatgcg aaggcgacgt ggtcgtgatc accaagccgg ggacgacggc gggtgtcctg
     1801 gccgccgatt gcgttccggt cgtcgtgttg ggcgaggacg gtttggctgc gatccatgcg
     1861 ggatggcgcg gcctggtcgg gggcgcgatc gaggccgggg tcgcccgcgt cggccggccg
     1921 gtggcggcct gggtcgggcc ctcgatcaag gggtgctgtt acgaggttgg gcctgacgtg
     1981 gtcggcgcct tccacgagaa ggctcttccc gtcgccgacg gccggcatgt cgatacgggg
     2041 gccgccgcgt acgccctgct catgcgaacg ggggtcga
//
LOCUS       DATMED010000194         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_908575, whole genome shotgun sequence.
ACCESSION   DATMED010000194 DATMED010000000
VERSION     DATMED010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908575"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VNP73_04855"
     CDS             <1..556
                     /locus_tag="VNP73_04855"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWL65285.1"
                     /translation="PEVLKRMGRWGARDRFERMIDRIRSADPLAGLRATFILGFPGEK
                     DEDAFAVESFVDDSDLDWIGTFTYSREEGTRSYPFEDQVPDEVARERVERVAGVADRA
                     MERRARSLVGKRLKVLVERFDVEENVWTGRSQREAPEIDGEIRFTTTRRPKVGGYVEV
                     ELTATDGADLVGVGSADTVPSARG"
     gene            574..>2077
                     /locus_tag="VNP73_04860"
     CDS             574..>2077
                     /locus_tag="VNP73_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65286.1"
                     /translation="MERPWRRVLPAAVSLALVSSALFSATPQVAAVAQDTTEPTPLFV
                     DQGLNASEEPAFKRSLRVLLKDRFVDMVITVRPRSRPRAGCTKRGVVYWVYAKRGTIC
                     FTRAPRGYGWRFSVQVVKGRNPVRKQSAWSLPTLKAERKASSYIEDPEPRNLIRSSRI
                     TYPYIYERIVAQLDGPRAGDFAIVPLNTADRGAPGAHGHPGLPQSRTTMIVAGRGARR
                     SPLNAKQEKKLRLRHPDIAPTVAKALGVRPYFVDTKEPATLLNGRPSETALLERQDGR
                     IQNRLLEPVFNTFVISIDGLRPEYISMMPNVSVLLDSECSPGGACATSYEHARGMMVT
                     ETNGNHTAMITGAYGERSGVFANEMWDQGADAAVDMESPSLNLAETLFDAIERQKPWL
                     TTASIMGKGKLRSLFDCTRTEDGECGPSDANPEKRAILHVEPDLVAGASTSPAGPLDC
                     PAEPGSGSDYTSNICTMDAAIELLRTEDPDFSFINLPEVDAFSHLFGPNST"
BASE COUNT          394 a          642 c          686 g          355 t
ORIGIN      
        1 accggaggtc ctcaagcgga tgggccgctg gggcgcgcga gatcggttcg agcggatgat
       61 cgacaggatc cggtctgcgg acccactggc gggcctgcgc gcaacgttca tccttggttt
      121 ccctggagaa aaggatgaag acgcattcgc cgtcgagagt ttcgtcgacg actccgatct
      181 ggactggatc ggcacgttca cgtactcgcg cgaggagggg acccgctcgt atccgttcga
      241 ggatcaggtg ccggacgaag tggcccgcga gagggtcgaa cgagtagccg gggttgcgga
      301 cagggcgatg gagcggcgcg cccggtcgct cgtgggcaaa cgattgaagg ttctcgtcga
      361 gcgtttcgac gtcgaggaga acgtctggac cggccgctcg cagcgcgagg cccccgagat
      421 cgacggcgag atccgcttca cgacaacccg tcggcccaag gtaggcgggt acgtcgaggt
      481 cgagctaacc gccaccgatg gggccgatct ggttggcgtg ggatcggccg acacggtccc
      541 gtccgcccgg gggtaagagg gacaatcaca gtcgtggaga ggccatggag gagagtcctg
      601 ccggcggctg tgagccttgc cctcgtctcg tctgccctct tctccgccac tccacaagtg
      661 gccgccgtag cccaggacac gacggaaccc acgccgctgt tcgtagacca aggtctgaac
      721 gcgagcgagg agcctgcctt caagcgatct ctgcgcgtcc ttttgaagga caggttcgtg
      781 gacatggtca tcacggtccg tccgaggagc cgcccgcgcg ccggatgcac gaagcgggga
      841 gtcgtgtact gggtttatgc gaaacggggc acgatctgct tcacgcgggc tccgcgcggc
      901 tacgggtggc gattcagcgt gcaggtcgtg aagggccgca atccggtcag gaagcagagc
      961 gcatggtcac ttccgacgct caaggcagag aggaaggcca gcagttacat agaggacccc
     1021 gagccgcgca acctgatccg cagcagcagg atcacttacc catacatata cgagcgcatc
     1081 gtcgcccagc tcgatggtcc tagagccggc gacttcgcca tcgtcccctt gaataccgcc
     1141 gaccgagggg ctccgggagc tcacggccac cccggtcttc cgcagtcccg aaccacgatg
     1201 atcgttgcag gtcgcggggc gcgccgttcg cctctgaacg cgaagcagga gaagaagctc
     1261 cgtctccggc atccagacat cgcacccacg gtcgctaaag ctctgggtgt tcgtccctat
     1321 ttcgtcgaca ccaaggaacc ggcgacgctg ctgaacggtc gcccctcgga gacggccctc
     1381 ctcgagcgcc aggatgggcg gatccagaac cgattgctcg aaccggtctt caacacgttc
     1441 gtgatctcga tcgatgggct gcggcctgag tacatcagca tgatgccgaa cgtgtcggtg
     1501 ctactcgact ccgagtgttc gccgggcggt gcatgcgcga cgtcttacga acatgcccgc
     1561 ggcatgatgg tgaccgagac caacgggaat cacaccgcga tgatcacggg tgcgtacggc
     1621 gagcgatccg gtgtctttgc taacgagatg tgggatcaag gcgcggatgc ggccgtggac
     1681 atggagtctc cgtccctgaa cctcgcggag acgctcttcg acgctatcga gaggcagaag
     1741 ccgtggctca cgactgcatc catcatgggg aaagggaagc tgcggagtct gttcgattgc
     1801 acacgcaccg aagacggcga gtgcgggccc agcgacgcga atccagagaa gcgagcgatc
     1861 ttgcatgtcg agcccgatct ggtcgccgga gctagtacgt cgccggccgg ccccctcgat
     1921 tgtcccgccg agcccggaag tggttcggac tacacgtcga acatctgcac gatggatgcc
     1981 gcgatcgagt tgctgcggac cgaggatccg gacttctcgt tcatcaacct gcctgaggtc
     2041 gacgctttct cacacctctt cgggccgaac agcactc
//
LOCUS       DATMED010000195         3818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3223849, whole genome shotgun sequence.
ACCESSION   DATMED010000195 DATMED010000000
VERSION     DATMED010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223849"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="VNP73_04865"
     CDS             <1..1552
                     /locus_tag="VNP73_04865"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HWL65287.1"
                     /translation="TIDAYDWIHRTRANPPHEPAPGDNCASAAARPYLYEGTFAHEYQ
                     HLLEYYRDADETTWLNEGLADLAQTVTGYVDPSIPVTDIGFDGHIQAFSGWLELQTDA
                     NPIPRAGGPENSLNLWDEAEPQQPGEVLSDYGATYTFLELLHHRYGRAFIKKLHRHPL
                     NGIESLGTLLADVKPRRTARRVLHDWSAMIALDGAIDDGAALTGGPAGAFKTRSMHSS
                     VNWDNDDTYSYPGAPPNGSDFVRLRDGDGNYLNASQIESITFDGSDGLPAKPVEWVVE
                     EDPPGHSNDEALASGSVLNGDRGIVAEVTVPQQNSTLTFETRFEIEESFDFGFVQVST
                     DGGRSYRSLSNTATTDDADPSAQERMFDNLPGLTGSSDWIELSFDLSQYAGQEVLLAF
                     RYMTDNTVSLGGWWIDDVRVGSTTVSDGDSLRDWRTPTQVSPDPVEGFSLQLIAYNDA
                     HTQAGHTLVPLGPGFTATLEGAALDFMPDDATTVAALVMYEESTELIQQYAHYSLDVN
                     GVRQPGGS"
     gene            1626..3455
                     /gene="selB"
                     /locus_tag="VNP73_04870"
     CDS             1626..3455
                     /gene="selB"
                     /locus_tag="VNP73_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HWL65288.1"
                     /translation="MHVVGTAGHVDHGKSTLVEALSGMDPDRFEEEKRRGLTIDLGFA
                     WLELPSGNEIGIIDVPGHERFIRNMLAGAGGSSICLFVVAANEGWRQQSAEHLAILDV
                     LGVSNGVVAVTKSDLVDEDVLQEVVSDVAERLKASTLRGIPIVACSATTGNGLAELTE
                     AIDRVVAAAPPVVDRGTPRLWIDRSFTISGAGTVVTGTLMGGSLSIGESVAVARADVT
                     ARIRSIQTHKRSHDVVEPGNRVALNLAGVERSRIERGDAVVKENQWLSTSKLYAQVRV
                     LDPEISGTEHALTERGAHLMYTGTAETPVRIRLVQGHQISAGASAFAELSLRDALPLK
                     RGDHFVLRDAGRVLTFGGGRVLDPLPALPRRDPRLPALLDALASEDDTHALATLVATE
                     EMVSEDLALLRAGAGPADTGAERLGNWLVSAAKLASLKADLTAAVQEHHRDQPLAAGM
                     PKERARRIVLQETPDAFDPLVAVTTGVVTEGDVIKLSTHRVSLDPEQERRRLATIEAL
                     EAGGFSPPPTSELEADPPLLRAMVQSGDVVQIGDFHLTAGRAAEVRKLVRNTIEASGP
                     VTVAAIRDLLGTSRKYAVPLCEWLDQTGATRRRGDVRVLGPHE"
     gene            3495..>3818
                     /locus_tag="VNP73_04875"
     CDS             3495..>3818
                     /locus_tag="VNP73_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65289.1"
                     /translation="MKTSRIAALFVAAAVGSSAGAAAAAEIDASSKIGLKYRPAKGAI
                     AGRVSVPTTTEAVEKICTRDRVVKLVKIESGKKRVVKKTATRKLGRYGFQVAKAGRYQ
                     AKVLSK"
BASE COUNT          735 a         1206 c         1239 g          638 t
ORIGIN      
        1 gaccatcgat gcctacgact ggatccaccg cacgcgggcg aatcctccac atgagccggc
       61 gcccggggac aactgcgctt cggcggcagc acggccctat ctgtacgagg gaacgttcgc
      121 gcacgagtac cagcacttac tcgagtacta cagagacgcg gacgagacca cgtggttgaa
      181 cgagggattg gccgatctcg cccagacggt caccggctat gtagatccct cgatccccgt
      241 caccgacatc ggattcgacg gccatatcca ggcgttctcc gggtggctcg agctgcagac
      301 cgatgcgaac ccgatcccgc gtgcgggggg gccggagaac tcgctgaacc tgtgggacga
      361 ggccgaacca cagcaacccg gagaggtgct ctcggactac ggcgcgacct acacgttcct
      421 ggaacttctg catcacaggt acggacgggc attcatcaag aagctccaca ggcatcccct
      481 caacgggatc gagagcctgg gcaccttgct ggccgacgtg aaaccccgac gcacggcgcg
      541 ccgagtgctg cacgattggt cggcgatgat cgccctcgat ggtgcaatcg acgacggggc
      601 cgcattgacc ggtgggccgg ccggcgcctt caagacgcgc tcgatgcatt cctcagtgaa
      661 ctgggacaac gacgacacgt actcgtaccc cggcgcacct ccgaatggat cggacttcgt
      721 ccgccttcgg gatggggacg ggaactacct caacgcgtcg cagatcgagt cgataacgtt
      781 cgacggtagc gatgggctgc cggccaagcc ggtggaatgg gtcgtcgaag aggacccgcc
      841 gggacattcg aacgatgagg ctctggcgtc gggctcggtt ctaaacgggg accgcgggat
      901 agtggccgag gtcaccgttc ctcaacagaa ctcgacactc accttcgaga cccgcttcga
      961 gatcgaagag agcttcgact tcggattcgt ccaggtctca acggacgggg gccgtagcta
     1021 tcgcagtttg tccaacactg cgacaactga cgacgccgat ccttcggcgc aggaacggat
     1081 gttcgacaac ctgccggggt tgactggatc ttcggattgg atcgaactca gcttcgacct
     1141 gtcgcagtac gccggccaag aggttctgct ggctttccgt tacatgaccg acaacacggt
     1201 gagtcttgga ggttggtgga tcgacgacgt gagggttggt agcacgacag tttcggacgg
     1261 cgactcgctg agggactgga gaacccccac gcaggtctca cccgaccctg ttgaaggttt
     1321 ctcgctgcaa ttgatcgcct acaacgacgc tcatacgcaa gcgggacaca cgctcgttcc
     1381 tctgggcccg ggattcaccg cgactctcga gggggccgcg ctggacttca tgccggacga
     1441 cgcgacgacg gtggcggcgc tggtcatgta cgaagagtcg acggagctca tccagcagta
     1501 cgcccactac tcgctggatg tgaacggagt gagacagccc gggggctcgt agccgatcca
     1561 aacctagccc gaacgccgaa cgtctcgccg ttcggcgaga acaaacggtt gcttaggctc
     1621 gggtcatgca cgtcgtcggc accgccgggc acgtcgatca cgggaaatcg accctcgtcg
     1681 aagcactatc ggggatggat cccgatcgct tcgaagaaga gaagcgtcgc ggtctcacga
     1741 tcgacctcgg cttcgcgtgg ctggaactgc cgtccgggaa cgagatcggg atcatcgacg
     1801 tcccgggtca cgaacgattc atccgaaaca tgctcgccgg agcgggagga agttcgatct
     1861 gcctcttcgt cgtcgcggca aacgaagggt ggaggcagca atcggccgag cacctcgcga
     1921 tccttgacgt cctcggggtg tcgaacgggg tcgtcgccgt gacgaaatca gatctggtcg
     1981 acgaggacgt cctgcaggaa gtcgtatccg acgtcgccga gcgcctcaag gcatccaccc
     2041 ttcgaggcat cccgatcgtg gcgtgttcgg cgacgaccgg caacggtcta gccgagctga
     2101 ccgaagcgat cgaccgcgtg gttgccgctg cgccgcctgt tgtcgaccga ggaacaccac
     2161 gcttgtggat cgatcgctct ttcacgatct cgggggcggg aacagtggtc accggaacac
     2221 tgatgggagg ctcgctctcc attggagaga gcgtggccgt cgctcgggcc gacgtcactg
     2281 ctcggatccg atctatccag acccataagc gctcgcacga tgtggttgag cccggcaacc
     2341 gagtggctct gaaccttgcc ggagtcgaga ggtccaggat cgagcgcggt gatgcggtcg
     2401 tcaaagagaa ccaatggctt tcgacgagca agctgtacgc ccaggtgcgt gtgctcgatc
     2461 ccgagatctc cggcaccgag cacgcgctga ccgagcgagg tgctcacctg atgtacacgg
     2521 gaaccgcgga gactccggta aggatccgct tggtgcaggg ccaccagatc tccgccgggg
     2581 cgtcggcatt cgccgaactt tcgttgcgcg atgcgctacc cctgaagcga ggagatcact
     2641 tcgtactgcg agatgccggc cgtgtgctga cattcggggg gggccgcgtc ctcgaccctc
     2701 tcccggccct gccgcgtcgc gaccctcgtt tgccggcgct gctcgacgcg ctggcatccg
     2761 aggacgacac gcacgcactc gcaaccctcg tcgcgacgga agagatggtg tcggaggatc
     2821 ttgcccttct ccgtgccggg gcagggccgg ccgacacggg ggccgagcgg ctaggcaact
     2881 ggctggtatc ggcggcgaag ctcgcctctc tcaaggccga cctcactgcc gccgtccaag
     2941 aacatcaccg agaccaacct ctggctgccg ggatgccgaa ggaacgggcg cggcggatcg
     3001 tgctgcagga gacccccgac gctttcgacc ctctcgtagc ggtcacgacc ggggtcgtca
     3061 ccgagggcga cgtgatcaag ctgtcgaccc accgggtgag cctcgatccc gaacaggagc
     3121 gtcgtcggct tgcgaccatc gaggcgctcg aggccggagg cttcagcccg cccccgacct
     3181 ccgagctcga ggcagaccct cctctgctcc gggcgatggt ccagtcaggc gacgtggttc
     3241 agatcggtga tttccacctc accgccggcc gggcggccga ggtccggaag ctggttcgga
     3301 acacgatcga ggcgtcggga cccgtcaccg ttgcggccat ccgggacctc ctcggcacgt
     3361 ccaggaaata cgcggtgcca ctgtgtgagt ggctagatca gaccggtgcg acgaggcgcc
     3421 gcggcgacgt ccgggtatta ggtccccacg aatgagggat taaagacgac cctcgcgacg
     3481 ccgatagcca cttcatgaag acttcgagga tcgcagcgtt gttcgtagca gccgccgtgg
     3541 ggtcgtccgc cggcgccgca gccgccgccg agatcgatgc atcgagcaag atcggtctca
     3601 agtaccggcc cgccaagggc gccatcgcag gccgcgtttc ggtcccgaca acgacggaag
     3661 ccgtcgagaa gatctgcacc cgcgaccgag tcgtgaagct ggtgaagatc gagtccggca
     3721 agaaacgcgt cgtgaagaag acggccactc gcaagctggg acgctatggg tttcaagtag
     3781 ccaaagccgg ccgctaccag gcgaaggtgc tttcgaag
//
LOCUS       DATMED010000196         9682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4369729, whole genome shotgun sequence.
ACCESSION   DATMED010000196 DATMED010000000
VERSION     DATMED010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4369729"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..935
                     /locus_tag="VNP73_04880"
     CDS             144..935
                     /locus_tag="VNP73_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65290.1"
                     /translation="MKRVLLLLLTLFPLAACRSDSIALQYTFGNDEVRYRMEAEANAR
                     WNLDGPGRGSYTAVFDVTERVTEDAGEGAIVAVTMDPVEVEEEGLPSPGLESRSFSLR
                     SGPNGEVLEVLEVDGVPAGVLDNDQIGLIATYRPPLPLQPVKLNGTWNAEQQLELPSV
                     FQQITTIGRLTRLDRDADGRLAQLAYTGSGPLVRSLELPQGQADLTGSTTIAIDARLD
                     IDEGLLREAHSSTQGSFDVRVLPPDQRAPIVGSVRLDLEVRLTKV"
     gene            complement(932..1501)
                     /locus_tag="VNP73_04885"
     CDS             complement(932..1501)
                     /locus_tag="VNP73_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HWL65291.1"
                     /translation="MGRHSSGRQWPFVRSIIGWSLPWLLIAGVVAVGVWMIVDYTNRP
                     ETGPTAAREEEPTPEETISFEPEPKEEESITPIEETPEPKKEKERSPDRDSKLIVEGV
                     TVQVLNGTKAPEAGDEMAARLGELGFQIVAVETTSKNYRDTTVFWSFPEAERAAKRLA
                     ARFGWAVDEKPGNLADTVSLHVVVGKDEL"
     gene            complement(1514..1783)
                     /locus_tag="VNP73_04890"
     CDS             complement(1514..1783)
                     /locus_tag="VNP73_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HWL65292.1"
                     /translation="MSALTGRDIDMLDFERTWWKHAGVKEQAIKERFEMSATRYYQIL
                     NDLLEKPEAMDHDPILVKRLKRLRVYRQRQRVARLLGAEYLPGKR"
     gene            complement(1865..3148)
                     /locus_tag="VNP73_04895"
     CDS             complement(1865..3148)
                     /locus_tag="VNP73_04895"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWL65293.1"
                     /translation="MTVEDDLHQQGHDPGDSAWETALAQLDEAAELMGLAPGVHQTLR
                     APKRALIVSVPFRMDDGSTRVYQGYRVHHNVTRGPAKGGIRYHPNVGLGEVKALAMWM
                     TWKCAIAGIPFGGAKGGVSVNPKVHSKGELERMTRRYASEILPLIGPERDIPAPDMNT
                     NEEIMAWIMDTYSINQGYSVPGVVTGKPVAIGGSKGRGGATSRGVMYMIFSTLKALGK
                     GIDEVSVAIQGYGKVGGHAAQLLHDAGCRVIAVSDAEGGLYREKGLDPEAINRHSDEA
                     GTVVGYPGADAISNEELLELECDVLIPAAIEGVITVKNADDIKARVVCEAANGPITFE
                     GDKVLNERGVFVVPDILANSGGVTVSYFEWVQDIQAYFWSEEDVNDRLRQIMERAFFE
                     VYDLANEKGLSMRQAAHWIGVGRVAEAHQTRGLFP"
     gene            complement(3199..3274)
                     /locus_tag="VNP73_04900"
     tRNA            complement(3199..3274)
                     /locus_tag="VNP73_04900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3239..3241),aa:Thr,seq:tgt)
     gene            complement(3748..4383)
                     /gene="sigH"
                     /locus_tag="VNP73_04905"
     CDS             complement(3748..4383)
                     /gene="sigH"
                     /locus_tag="VNP73_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HWL65294.1"
                     /translation="MPTELKNLEDEMLVELARAGDEQALEDLLNRYRHYARAKARTYF
                     LAGADKEDIVQEGMIGLFKAIRDFQSDKNTAFRAFAELCITRQIITAIKTATRQKHIP
                     LNSYVSLNKSASVHDDEPPLEEALSQGSIDDPAELVISAEQIENIKVSMGQLLSDLET
                     EVLQLYMDGKSYQQIADMLGRHVKSIDNALQRIKRKLEQHLTVQNAEMLAQ"
     gene            complement(4438..5202)
                     /gene="rlmB"
                     /locus_tag="VNP73_04910"
     CDS             complement(4438..5202)
                     /gene="rlmB"
                     /locus_tag="VNP73_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWL65295.1"
                     /translation="MPRRTDHPDTHELIAGRRPVLEALAAGQRVERVILAEGVRSTGP
                     IAEIRKRATELSIPVKTMPRKELERLAPDLNHQGVVAEAGRFRYASFESLFQAGERLL
                     LFLDGVTDPHNLGALLRSADGAGFSGVVIPTRRSAAVTPTVRKVAAGAAEVVPVARVA
                     SLGTALEQAKRNGFWVVGLDGKATEDLWSSDLLEAPLALVLGAEGRGISQGIRGRCDG
                     FVRIPSVGALASLNVSVAGALAMFELARRRSASDTL"
     gene            complement(5186..6628)
                     /gene="cysS"
                     /locus_tag="VNP73_04915"
     CDS             complement(5186..6628)
                     /gene="cysS"
                     /locus_tag="VNP73_04915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWL65296.1"
                     /translation="MPLHVFDTLRREAVEFVPRDPGRVGLYCCGMTVYNYAHIGNMRT
                     FVWFGFISSYLEYRGFDVTYVMNYTDIDDKIIERANIEKVPPDAITAKYIAAFEEDLR
                     NLGARAPDIVPRATGHIDGMIKAIEGLIEKGFAYEAEGNVWFSVEKFSDYGKLSKRSL
                     EDLKTQERIEPHSSKQHPVDFSLWKAAKEGEPAWESPWGPGRPGWHIECSVMSEKYLG
                     MGFDLHGGGSDLIFPHHENEIAQAEALTGEEPFARYWLHAGMVQMDTEKMSKSLGNVV
                     TAREILKQYPGEVVRYWMLQGSYRSQVVFTENAMADAAQSYDRWRIFLDASRHALGDA
                     MPEPTPVKRTDDSDNEPPTGYVRRFIDAMDADFNSAEAFATVHELVKEGNKLLESFQR
                     GEAEAGDLVELIHSFYEITAVLGFGFESTHDSELMGGLIGYLLELRETARAEKAFARA
                     DEIRDRLESLGVTIEDTPSGARWRFGAAAN"
     gene            complement(6663..7133)
                     /gene="ispF"
                     /locus_tag="VNP73_04920"
     CDS             complement(6663..7133)
                     /gene="ispF"
                     /locus_tag="VNP73_04920"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWL65297.1"
                     /translation="MRVGLGFDAHGFDDSRSLVIGGVTIEDSPGLAGHSDADVLCHAI
                     ADALLGAARVGDLGDVFPGTDEWKDASSLDLLAHTAAVLERTGWKIVNIDATVVAEQP
                     RLGPHRARMTENIATALGIPVGEVSVKATTTDGLGFAGRREGVGAMAVALVDRA"
     gene            complement(7133..7822)
                     /gene="ispD"
                     /locus_tag="VNP73_04925"
     CDS             complement(7133..7822)
                     /gene="ispD"
                     /locus_tag="VNP73_04925"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWL65298.1"
                     /translation="MSPGKSGSLGAILAAGGIGERARDSDRDPPKQFRQLGGRTVIEW
                     PLKALRGAACNPIVVVLPEPWIGTAETLMSGPDLIFCAGGATRQDSIHNGLEHIESDI
                     VVVQDASRPLITAELIRRVADTTSSADAVIAAVPMDETLKQAEDGRVIKTIDRSTIWK
                     AQTPAAFRTEVLKEAHRRAKEEGFVGTDEAQLIERYGGSVQIVPGSRRNLKITWAEDF
                     ELADALLKVSE"
     gene            complement(7843..8325)
                     /locus_tag="VNP73_04930"
     CDS             complement(7843..8325)
                     /locus_tag="VNP73_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWL65299.1"
                     /translation="MSFRVGDKVVYPHHGAAIIESVENKELFGEKRKYYILKLAYGDL
                     TLMVPTDSTDEVGLRQITPAKEVPKVLKVLRKNEPTTNTTNWSRRFKANVEKLRSGDI
                     YQVADVVRSLHQRDKEKGLAAGEKRMLTKARQILVSELTFSKNCNEEDAEKMLDEVLD
                     "
     gene            complement(8438..>9682)
                     /locus_tag="VNP73_04935"
     CDS             complement(8438..>9682)
                     /locus_tag="VNP73_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWL65300.1"
                     /translation="ASRGPGEPAAQSTPLGQTSPTAARHPTGIDELDRALGGGIVAGS
                     ITLLAGEPGIGKSTLVLQMLSGLAAGGLDGLLVTAEESLEQVAGRAQRLHAMGETKGV
                     ATDRIADLFAACERDKPHVLVVDSIQAVSDGAFDQPAGSLVQVRECAASLARFAKSTG
                     TAVVLVGHVNKDGVVAGPKTLEHLVDSVLSLDGDRTGSLRMLRCLKNRFGSCEETGVF
                     LMEPDGLVPVPDPSTMLLADRVTDACGSTVFCGLQGTRPYLAEVQALVTKGNNDAPGR
                     RVAIGFDSRRLSLLLAVLSKQSSKIHLGGADVFVATSGGITIREPAADLAFCLAIQSA
                     INDVPVDPSMVAIGEVGLAGEIRRVPNVRRRLAEAKRLGFERAVVPRGSDVDDLGLVI
                     SEVQDVRSAFTLAIRTKRVTAA"
BASE COUNT         1717 a         3093 c         2913 g         1959 t
ORIGIN      
        1 gcggtcgggc tcgtcgacga tcggagcgag agtgccgtcg taatccagcc acaatccgga
       61 cacttgtgga tcgttcaacc acggcccgag ttcatcggtc atcggaacga gggtagcccg
      121 gcccccctga ctacactcaa cgtatgaaac gcgtgctcct cctgcttctc accctcttcc
      181 cgctggccgc gtgcagatcg gactcgatcg ctcttcagta cacgttcggc aacgatgagg
      241 tccgttacag gatggaagcc gaagccaacg cgcgttggaa cctcgacggg ccgggccggg
      301 gctcctacac agcggtcttc gacgtcaccg aaagagtgac cgaagatgcc ggcgaaggag
      361 cgatcgtggc agtgaccatg gatccggtgg aggtcgaaga agaaggcttg ccttcacccg
      421 ggctcgagtc gcgttcgttc tcgctgagga gcggtcccaa cggtgaggtt ctcgaagtgc
      481 tcgaggtcga cggcgttccg gccggtgtgc tcgacaacga ccagatcgga ctgatcgcta
      541 cctaccggcc gccactcccc ctgcaacccg tgaaactcaa cgggacctgg aacgcggagc
      601 aacagctgga acttccctcc gtgttccaac agatcacgac gatcggtcgg ttgacacggc
      661 tcgatcgcga tgccgatggg cggctcgctc aattggccta cacgggaagc ggcccattgg
      721 tacgaagctt ggagttgccc caggggcagg ccgacctaac gggatcgacg accatcgcta
      781 tcgacgctcg actcgatatc gacgagggac ttctccggga ggctcattcc tcgacacagg
      841 gatcgttcga cgtgagagtg ctgccccccg accaacgtgc accgatcgtc gggtcggtcc
      901 gtctggatct cgaggttcgc ctgacgaagg tctagagctc gtccttaccg acgaccacgt
      961 gtagagaaac ggtgtcggcg aggttgcccg gcttctcgtc taccgcccaa ccgaaccggg
     1021 ccgccagacg cttggccgcg cgttcggcct ccgggaagga ccagaacacc gtggtgtccc
     1081 tgtagttctt cgaggtcgtc tcgaccgcga cgatctggaa cccgagctct ccgagcctcg
     1141 ctgccatctc gtcgccggct tcgggtgcct tcgtaccgtt gagcacctga acggttaccc
     1201 cttcgacgat cagcttgcta tcgcggtccg gcgaccgctc cttttccttc ttcggttcgg
     1261 gggtctcctc gatcggagtg atcgactcct cttctttggg ttcgggctcg aagctgatcg
     1321 tctcctcggg ggtcggctct tcctcgcgtg ccgccgtcgg tccggtctcc ggtcggttcg
     1381 tgtagtcgac gatcatccac accccgaccg cgacgacacc cgcgatcagt aaccacggaa
     1441 gtgaccaacc gatgatcgaa cgaacgaagg gccactgtcg ccccgacgag tgccggccca
     1501 tggcgcggcc tcgctatctc ttgccgggga ggtactcggc cccgagcaga cgagccactc
     1561 gctgacgctg ccggtacacg cgcagacgct tcagtcgctt aacgaggatc gggtcgtgat
     1621 ccatcgcttc gggcttttcc aagagatcgt tgagtatctg gtagtagcgg gtcgcagaca
     1681 tctcgaaacg ctccttgatg gcctgctcct tgacgccggc gtgcttccac caagtgcgtt
     1741 cgaaatcgag catgtcgatg tctcgtccgg taagggcgct catgcctaga gattagtccg
     1801 ggtgatttcg cgagttgcgg attttgggcc gggacaggcg gtcacttttc gcgggggttg
     1861 gcccttaagg gaacaagcct ctggtttggt gagcctcggc gacccggccg actccgatcc
     1921 agtgggccgc ctggcgcatc gacaacccct tctcgtttgc gaggtcgtag acctcgaaga
     1981 acgccctctc catgatctgg cgcaggcgat cattcacgtc ttcctccgac cagaagtacg
     2041 cctggatgtc ctgcacccac tcgaagtagg aaacggtcac accgcccgag ttggcgagga
     2101 tgtcgggcac aacgaagacg ccgcgctcgt tcagcacctt gtctccctcg aacgtgatcg
     2161 gaccgttggc ggcctcgcat acgacgcggg ctttgatgtc gtcagcgttc ttgacggtga
     2221 tcacgccttc gatcgcggcc gggatcagaa cgtcgcactc gagctcgagg agttcttcgt
     2281 tcgagatcgc atcggccccc ggatagccga cgacggtgcc ggcttcgtcg ctgtgccggt
     2341 tgatcgcttc ggggtcgaga cccttctctc gataaaggcc gccttcagca tcggaaaccg
     2401 cgatgacgcg gcatccggca tcgtgcagaa gctgtgccgc gtgacctccg accttgccgt
     2461 agccctggat cgcgacactc acctcgtcga tccccttgcc gagtgccttg agcgtggaga
     2521 agatcatgta catgacgccg cgagaggtcg cgccgccgcg gcccttcgac ccgccgatcg
     2581 ctaccggctt accggtcacg acccccggaa ccgagtagcc ctggttgatc gagtacgtgt
     2641 ccatgatcca ggccatgatc tcttcgttcg tgttcatgtc gggggccggg atgtcgcgct
     2701 cggggccgat caaaggaagg atctcggacg cgtagcggcg ggtcatccgt tccagctcgc
     2761 ctttgctgtg aaccttcggg ttgacactga cgccaccctt cgctcctccg aacgggatgc
     2821 cggcgatcgc gcacttccac gtcatccaca tcgccagcgc cttgacctcg ccgaggccga
     2881 cgttggggtg atagcggatg cctcccttgg ccggcccgcg ggtcacgttg tggtggacgc
     2941 ggtagccctg gtagacgcgc gtcgagccgt catccatgcg gaagggaacg gagacgatca
     3001 gcgcgcgctt gggagcgcga agggtttggt ggacgccggg ggcaaggccc atgagctcgg
     3061 cggcttcgtc gagctgagcg agagccgtct cccaggcgga gtctccgggg tcgtgcccct
     3121 gctggtgcag atcgtcttcg accgtcactt gtccattatg ggccgttcac aggagattga
     3181 aacgaaactg gatctggttg gagccgggtg tcgggatcga accgaccgcc tgcgcattac
     3241 aaatgcgctg ctctaccaga tgagctaacc cggcggaaag ctgataatcc ttcgcccagc
     3301 gtattccttt ggcttggcta ttccgagctg gttcaacccg gaggctaccg gctacatttc
     3361 ccacctcccg caccggtacg agatacacct cgtcgttctc agggcagtaa acgccaaaga
     3421 cgtcagctga accttggtag ccgtgacggt acgcccgcat accttggttg ttggggtggt
     3481 gataggtgac gctgcaagtc gggaaaacga tcgcaccctt gcgaagccgt cctgtcttgc
     3541 attggacgcg gatgaacttc accccggtat cgatcagcag gtcgtaacgg ctgttctctc
     3601 cgaagggcat gaggatctcg gcctttggat agacggcgac gaggcgagcc aagaccatgg
     3661 cttcggtggc gtcgcctacc gctttgggat ggagtctttt cttttgcacc ctaggagtct
     3721 agggaggggg tgtgacacaa agtctggcta ctgggcgagc atctccgcgt tctggaccgt
     3781 tagatgctgc tcgagcttgc gcttgatgcg ctgtagtgcg ttgtcgatcg acttaacgtg
     3841 gcgtccgagc atgtcggcga tctgctggta cgacttgccg tccatgtaga gctgcaacac
     3901 ctccgtctcg agatccgaca gcaactggcc catcgagacc ttgatgttct cgatctgttc
     3961 cgcggagatc acgagctcgg ccgggtcatc gatcgaaccc tggctcaacg cttcttcgag
     4021 tggcggctca tcgtcgtgca ccgacgccga cttgttgagg gaaacgtagg agttgagtgg
     4081 gatgtgtttc tgacgggttg ccgtcttgat ggcggtgatg atctggcggg tgatgcagag
     4141 ttctgcgaac gctctaaagg cggtgttctt gtccgactgg aaatcgcgga tcgctttgaa
     4201 gaggccgatc atcccttcct gcacgatgtc ttctttgtcc gcacctgcta ggaaataggt
     4261 acgtgctttg gcgcgggcgt agtggcgata gcggttcaag aggtcttcga gagcctgctc
     4321 gtcgcccgcg cgcgcgagtt cgacgagcat ctcgtcctct agatttttga gttcagttgg
     4381 catccgccct ccgcattgag agcccgcttc cgcatccgca cctcgccaac cccctgctca
     4441 aagggtatcg gaagcggagc gtcgtcgtgc gagttcaaac atggcaagcg ctccggcgac
     4501 cgaaacgtta agggacgcaa gggctccgac gctagggatc cgaacgaaac cgtcacacct
     4561 gcccctgatc ccctgcgaaa tccctcttcc ctcggccccc aggaccagag cgagtggcgc
     4621 ttcgaggaga tcgctcgacc acaggtcctc cgtggccttc ccgtccaggc cgacgaccca
     4681 gaatccgttt cgcttcgctt gctccagggc cgtccccagg ctggcgaccc gggcaacggg
     4741 aaccacctcg gcggcgcctg ccgcgacctt tcggaccgta ggggtcaccg ccgccgacct
     4801 ccgagttggg atcacaaccc cggagaaccc tgccccgtcc gcggatctca atagggcgcc
     4861 gaggttgtgc gggtcggtga cgccgtccag aaacaggagg agcctctcac cggcctggaa
     4921 cagactctcg aacgacgcgt agcggaagcg ccccgcttcg gcaaccacgc cctgatggtt
     4981 cagatcgggg gcaagtcgct ccaactcctt cctcggcatc gtcttgaccg ggatcgacaa
     5041 ctcggtcgct cgcttcctga tctcggctat cggacccgtc gacctgacgc cttccgcgag
     5101 gatcacgcgt tcgacgcgct ggcccgcggc gagagcctcg agcaccggtc tgcggcccgc
     5161 gatcagttcg tgagtgtccg ggtgatcagt tcgccgcggc accgaaacgc caacgagcac
     5221 ccgaaggtgt gtcttcgatc gttacgccga ggctttccag ccggtccctg atctcgtccg
     5281 ctctggcgaa tgccttttcg gcgcgagcgg tttcacgcag ctcaagtagg tatccgatca
     5341 gtcctcccat gagttcggag tcatgtgttg actcgaatcc gaacccgagg acggcggtta
     5401 tctcgtagaa gctgtggatc agctcgacca ggtcgccggc ctcggcctca ccccgctgga
     5461 aggactccaa cagcttgtta ccttccttca cgagctcgtg cacggttgcg aatgcttcgg
     5521 ccgagttgaa atcggcgtcc atggcatcga tgaaccgccg gacgtaaccc gtgggtggct
     5581 cgttatccga gtcgtccgtt cgtttgactg gtgtcggttc cggcatggcg tcgccgagcg
     5641 catgtcggga agcgtcgagg aagatcctcc acctgtcgta ggactgcgcc gcgtcggcca
     5701 tcgcattctc agtgaacacg acctggctgc gataagagcc ctggagcatc cagtaacgaa
     5761 cgacctcacc cggatactgc ttgaggatct cgcgagccgt aaccacgttg ccgagcgact
     5821 tggacatctt ctctgtgtcc atctgcacca tgccggcgtg caaccagtaa cgagcgaacg
     5881 gttcttctcc ggtcagcgcc tcggcttgcg cgatctcgtt ctcgtgatga ggaaagatca
     5941 ggtcactgcc accaccgtgc aagtcaaagc ccatcccgag atacttctca gacatcaccg
     6001 agcactcgat gtgccacccg ggccgacccg gtccccacgg tgactcccac gcgggctccc
     6061 cttccttcgc cgccttccac agtgagaagt ccacgggatg ctgtttcgaa gagtgaggct
     6121 cgatccgttc ctgtgtcttg agatcctcga gcgagcgctt cgacagcttg ccgtagtcgc
     6181 tgaacttctc gaccgagaac caaacgtttc cttccgcctc gtaggcaaag cccttctcga
     6241 tcagaccctc gatggccttg atcatgccgt cgatgtgtcc ggtcgcacgc ggaacgatgt
     6301 ccggagcccg tgcccccagg ttgcggagat cctcttcgaa agcggcgatg tacttcgccg
     6361 tgatcgcatc tggtgggact ttctcgatat tcgcccgctc gatgatcttg tcgtcgatgt
     6421 cggtgtagtt catgacgtaa gtaacgtcga aaccccggta ttcgagataa ctactgatga
     6481 agccgaacca aacgaacgta cgcatgttgc cgatgtgcgc gtagttgtag acggtcatcc
     6541 cgcagcaata gagccccacg cgtccggggt cgcgtgggac gaactcgacc gcctcgcggc
     6601 gcaacgtatc gaatacgtga agtggcatgg tcgctagatt agttcgctcg ccggtagcaa
     6661 gatcaagctc tatcgacgag cgcaaccgcc atggcgccga ctccctctcg acgaccggcg
     6721 aacccgagtc cgtccgtggt cgttgccttc accgagactt ctcccacggg gatccccaag
     6781 gccgttgcga tgttctctgt catccgagcg cgatgggggc ccagcctcgg ttgctcggcg
     6841 accaccgttg catcgatgtt cacgatcttc cagccggtcc tctcgagcac cgcggcggta
     6901 tgagccagca gatccaggct ggacgcgtcc ttccactcgt ccgttcctgg gaagacgtcg
     6961 ccgagatctc cgacccgagc cgcacccaac agggcgtcgg cgatcgcgtg gcacagcacg
     7021 tcggcgtccg agtgtccggc aagccccggc gaatcttcga tcgtcactcc gccgatcacc
     7081 agagatcgag agtcgtcgaa tccgtgggca tcgaaaccta gaccgacccg catcattcgg
     7141 acaccttcag caatgcgtcg gccaattcga aatcctccgc ccacgtgatc ttcaggttcc
     7201 ggcggctgcc cggcacgatc tggacggaac ccccgtaccg ttctatcaat tgtgcttcgt
     7261 cggtcccgac gaacccttct tccttagcgc ggcggtgagc ttccttcaag acctcggtcc
     7321 ggaaggcggc aggcgtctgc gctttccaga tcgtcgaccg atcgatcgtc ttgatgacgc
     7381 gtccgtcttc ggcctgcttg agcgtctcgt ccatgggaac cgcggctatg actgcgtcgg
     7441 cagacgatgt cgtatctgca acacgtctga tcagttcggc cgtgatcagc ggtcgggacg
     7501 cgtcttgcac caccacgatg tcggactcga tgtgttcgag tccattgtgg atcgagtctt
     7561 gtcgcgtcgc cccgccggca cagaagatca ggtcgggccc ggacatcagg gtctcggcgg
     7621 ttccgatcca gggctcgggt aacaccacaa cgatcggatt gcacgctgca ccgcgcagag
     7681 ccttcagcgg ccactcgatc acggttctgc cgccgagctg ccggaactgc ttggggggat
     7741 ccctgtccga gtctctggct cgctcgccta tcccgcccgc ggcgaggatg gcacctaggg
     7801 atccagactt tcccggcgac atgagcgctg gggccagtcg tcttagtcga gcacctcgtc
     7861 gagcatcttc tcggcatctt cttcgttgca attcttcgag aaggtgagct cggaaacgag
     7921 gatctggcgt gctttcgtca gcatgcgctt ctcacctgca gccagacctt tttccttgtc
     7981 gcgctgatgc aggctgcgaa cgacgtcggc tacctggtag atatcaccgg agcggagctt
     8041 ctcgacgttc gccttgaaac gacgggacca gttggtcgta ttagtggtcg gctcgttctt
     8101 cctgaggacc ttgagaacct tgggtacttc cttcgccggc gtgatctgac gcaaaccaac
     8161 ctcgtccgtc gagtccgttg ggaccatcag agtcaggtct ccgtaggcga gtttcaatat
     8221 gtagtacttg cgcttctcac cgaagagttc cttgttctcg accgattcga tgatcgcagc
     8281 accatggtgc ggataaacaa ccttgtctcc gacacggaaa gacacgcttt acatccctcc
     8341 ctgcgaaagt cgcgggcgat ggaccggatg gatcttcggt gggttcggga gtacggcacc
     8401 tcccggttca gcaaggatac cacgcagcgc cggctcccta tgctgccgtg acgcgcttcg
     8461 ttctgatcgc gagcgtgaat gccgaacgta catcttgcac ttcggagatc accaaaccga
     8521 gatcgtctac atcactaccg cgcggcacga ccgctctttc gaagccgagt cgcttggcct
     8581 ccgctaggcg ccggcgcacg tttggaaccc tgcggatctc accggcaagc cccacctcgc
     8641 cgatggcgac catcgacgga tccaccggca catcgttgat cgccgactgg atggccaggc
     8701 agaacgcaag atccgccgcg ggctcgcgga tcgttatccc tcccgaggtc gccacgaaga
     8761 cgtctgcgcc gcccaggtgg atctttgagc tctgtttgga cagaactgca agtaggagag
     8821 acaggcggcg cgagtcgaat ccgatcgcaa cgcgacggcc gggtgcgtcg ttgttgcctt
     8881 tcgtaacgag ggcttggacc tcggccaggt aagggcgagt gccttgcaaa ccgcagaaga
     8941 cggtcgaccc gcaggcgtcg gtcaccctgt ctgccagcaa catcgtggac ggatctggaa
     9001 cgggaacgag accgtccggc tccatgagga acacacccgt ttcctcgcag gacccgaatc
     9061 tgttcttcag acaacggagc atgcggagcg acccggtgcg atctccgtca agactgagga
     9121 cggagtcgac gagatgttcg agcgtcttgg gtcccgccac cacgccgtcc ttgttgacgt
     9181 ggccgacgag gacaaccgcg gttcccgtgc tctttgcgaa gcgggcaagt gaggcggcgc
     9241 actcacggac ctggacgagg gagccggcgg gttgatcgaa ggctccatcg ctgaccgctt
     9301 ggatcgagtc gaccaccaac acatgaggct tatcccgctc gcacgccgca aagaggtctg
     9361 cgatccggtc ggtggcaacg cctttcgtct cgcccatcgc gtggagcctt tgagcgcggc
     9421 ccgccacctg ttcgagtgat tcctcggccg tcacgaggag cccgtccaag cccccggcgg
     9481 ccagccccga cagcatctgc aacacgaggg tcgacttgcc gatcccgggt tcgcctgcga
     9541 gcagcgttat cgaccccgcg acgatcccac cgcccagagc acgatcgagc tcgtcgatcc
     9601 cagtgggatg ccgggctgcc gtgggagacg tctgccccaa gggtgtcgac tgggcggccg
     9661 gctcgcccgg cccccgcgag gc
//
LOCUS       DATMED010000197         6687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_913173, whole genome shotgun sequence.
ACCESSION   DATMED010000197 DATMED010000000
VERSION     DATMED010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913173"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNP73_04940"
     CDS             <1..278
                     /locus_tag="VNP73_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65301.1"
                     /translation="VSIYGTFEPENDGPEFTAVVVPDVEVLDVLVPSSGGLGAGGGET
                     SITLALTPEAAQEVVFSQEHGKIWLALMAPDSGRPGKRIVTIRQVIR"
     gene            275..1447
                     /locus_tag="VNP73_04945"
     CDS             275..1447
                     /locus_tag="VNP73_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWL65302.1"
                     /translation="MTPLVLALGSDASFKDLVVRSLKLRDHEVDWKTSILEASETLAS
                     GPRLIVVGPDVAEADAIDFVDRTAQASPSTAVVLVRSNLQNGLVTAAMRAGARDVIDM
                     TRDADELHNVLERAFNWSSSLRHDDEERASGRILSVFSSKGGTGKSFLSTNLASAIAQ
                     RTGESTALVDIDLAMGDAFAYFGQEPENHIRDLIALGDKDRAGVLAAGTQLLENLWGY
                     GAPTDPAAEIMHGDEVGKLLGGVARTFDHVVVDVPGNYSDQVLAALDASECVLLVCSL
                     DVVGIRHLAKAIETLESIGLPKERFRIVLNRAGSKVGITPADVERVLQVKVHALVPSS
                     ILVPASLNKGVPVYIQEPKSEVAQSIGTLADSLVGTKPIPKSSSRPRSRRLFSRSK"
     gene            1512..2774
                     /locus_tag="VNP73_04950"
     CDS             1512..2774
                     /locus_tag="VNP73_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017836192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWL65303.1"
                     /translation="MMVRVQARLIEVLGPKLYDSSLSDEHLESLVLGRVQELLEEESS
                     TLTLTERNMLMRTIADGVLGLGPLEDLMRDPSVSEILVNGHDTIFVEREGKLHRTDAH
                     FLNEEQLRHVIDKTVARVGRRIDESSPYVDARLDDGSRVNAIIPPLAIDGPVLTIRKF
                     SAEPYVWDDLITFGTMTPEVKTLLEACVRGRLNILVSGGTGSGKTTSLNVLSSFIPTD
                     ERIITIEDAAELRLQQPHVVRLEYRPPNIEGAGEISIRHLVRNSLRMRPDRIVVGEVR
                     GKEALDMLQAMNTGHDGSISTIHANSPRDALARLETMSSMAGMDLGIRAIREQMSSAI
                     DLVIHQSRLKDGSRRFTHVTEIVGMEGEVITLQDLFVFDHSAGMDKNGRHLGALKPTG
                     LRPAFLDNLEEHGITIPPETFAAAGEKW"
     gene            2771..4648
                     /locus_tag="VNP73_04955"
     CDS             2771..4648
                     /locus_tag="VNP73_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWL65304.1"
                     /translation="MRKFVVVALLLTALAVAAPASAQTGALTIRKVDTEAFPKVTVTV
                     ASSGLDAFSAEQLFVQENGARIDILETDSLMSSGKQIDVVLVIDTSGSMLGDAMAAAK
                     RAAVDFVKALPDGVRVGTVTFSDRPHLIQGITTDHSSVIQRIRTLEATGETALYDGVA
                     MAAGLFSGPNQRNVVLLSDGGDTASASTLNAAAQAIRKSVASVFAVGLETTETDVAAL
                     KRLANVSRGSYASAANAELSTIYQNLATEIRGQFVVTYESAAGSEEVDLTIGMPGAID
                     EVTFLAPERIAPPRDVRPSEPAPSPVRGPAGMILSLTLFFLAVASIIFMASGSRARRK
                     RNEELARRFDVKGPTPASRTGDHSHRRLVPTRLGNAAGRAVHGSSMASRIDRKLEQAG
                     LKLTTGEFVVGTIGAGLAAALIGATLAGIAAAVLLGAVGLIGPFLWLSLSARGRTSRI
                     KGQLPDVLMILASSLRAGHSFLQALDAVAREIGAPSDHEFNRAVAEIRLGRPMGESLN
                     DLADRIGSDDFKWAVLAIGIQKEVGGNLAEVLDTVAATLRERDDVRRQVQTLSAEGRL
                     SAYILTALPFALGLYLQMINPEAVGLLFSTSLGRIMVGGGTTLLLLGTLWMRKMVKID
                     V"
     gene            4641..5576
                     /locus_tag="VNP73_04960"
     CDS             4641..5576
                     /locus_tag="VNP73_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWL65305.1"
                     /translation="MFDILLVMALGSAFLAVAIVVLVVDSTYENRKRAVKLLEAGITS
                     SAAPTANLREKELRKSFIQRVILPALGSFGRFGRRVTPFDSADRIEMRLKEAGSPQAW
                     DAARVVAVKLITGAFGLVASLTLAATQEATFAASVLVVGLGTWGSYMLPDLRLMQKAR
                     ERQNEIRKAMPDTMDLLTIIVEAGLGFDAALAHVIQKVPGPLADEYGRTLHEIRLGVP
                     RADALRNMADRTNIEEVRSFVTSMVQAESFGVSITKVIRAQSHDLRSKRRANAEKKAQ
                     QLAVKMIFPLIFCILPAVLVVAMGPGVIRIVQTFL"
     gene            complement(5580..6131)
                     /locus_tag="VNP73_04965"
     CDS             complement(5580..6131)
                     /locus_tag="VNP73_04965"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWL65306.1"
                     /translation="MEEAALDLFKKKGFARTSVDEIAAAADVSRSTFFRYYGSKEAVF
                     FDDTDTQGERFLDRLVRRPGTEGPLAAFREALIEVSAMAEEEDGTRDRAILRRELMLT
                     DPTLNGRRAEEFEKWTAKIAHAFAQRAGRSDADAEDRVAAAICMAATEEIGEEWRRLV
                     DTVGAATAIQTGFERVRRLIADV"
     gene            6299..6424
                     /locus_tag="VNP73_04970"
     CDS             6299..6424
                     /locus_tag="VNP73_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65307.1"
                     /translation="MSTDFLPMWAVGVAVGAAVLVAVGLVIWDRASKRKDRKNGD"
     gene            complement(6421..>6687)
                     /locus_tag="VNP73_04975"
     CDS             complement(6421..>6687)
                     /locus_tag="VNP73_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65308.1"
                     /translation="PDCGYNFERESGYWVGALIVNIAVAEIWFFVLLMAVLLASMPDV
                     AWGPLLVVALVTNGLLPIFFYPYSKSLWMAIDLYIHPIEPGDSD"
BASE COUNT         1312 a         2075 c         2049 g         1251 t
ORIGIN      
        1 acgtttccat ttacggcacg ttcgagccgg agaacgacgg gccggagttc accgcggttg
       61 tcgtcccgga cgttgaagtc ctcgatgtgc tggttccctc cagcgggggc ctgggggccg
      121 gcggtgggga gacgagcatc actctcgccc tgacgccgga ggccgcccag gaagttgtct
      181 tcagtcagga acacgggaag atctggctgg cactcatggc accggattcc ggtcgccccg
      241 gtaagcggat cgtcaccatt cgtcaggtga tccgttgaca cccctggttc tcgcgcttgg
      301 atcggacgcg tccttcaagg acctggtcgt ccgatcgctg aagctacgtg accacgaggt
      361 cgactggaaa acgtcgatac tcgaagcatc ggagacactc gcttccggtc cccgtctgat
      421 tgtggttggg cccgacgtgg ccgaagccga tgcgatcgac ttcgtcgata ggacggctca
      481 agcatctccc agcaccgcag tcgtgctcgt gcgcagcaac ctccagaacg ggctcgtcac
      541 ggcggccatg cgagcgggcg ctcgggatgt aatcgacatg acccgggatg ccgatgaact
      601 gcacaacgtc ctggagcggg ccttcaactg gtcttcgagt ctgcgccacg acgatgagga
      661 gcgtgccagc ggccggatcc tttcggtctt ctcttccaaa ggaggaaccg gcaagtcgtt
      721 cctgtcgacg aacctggcgt cggcgatcgc tcagaggacg ggagagtcga cggctctggt
      781 cgacatcgat ctggcgatgg gcgatgcgtt cgcctacttc gggcaggaac cggagaacca
      841 catccgcgac ctgatcgcgt tgggtgacaa agaccgggcc ggagtacttg cggccggcac
      901 acagctactc gagaacctgt ggggttacgg agctccgacg gatcctgctg cagagatcat
      961 gcacggggac gaggtcggca agttgctggg cggagtcgcc cgaacgttcg atcacgtcgt
     1021 cgtcgacgtg ccgggcaact actcggacca ggtgctcgct gccctcgatg cctcggaatg
     1081 tgtcctgttg gtctgctcgc tcgacgtagt cgggattcgc cacctggcta aggcgatcga
     1141 gacgctggag tcgatcggac tgccgaagga aaggttccgc atcgtcctca accgcgctgg
     1201 aagcaaagtg ggtatcaccc cggccgacgt ggagcgtgtc ctgcaggtca aagtccatgc
     1261 cctcgttcct tcgagcatcc tcgtcccggc atcgctcaac aaaggtgtcc ccgtctacat
     1321 ccaggagccg aagtccgagg tggctcagag catcggcact ctcgcggact cactcgtcgg
     1381 aacgaaaccc atccccaagt cgtccagcag gccgcgttcg cgccgcctct tcagtagatc
     1441 caagtaagga ggctccgatg tcactcgcac aacggttagc gcaaagcgaa cggcccgaca
     1501 aacacacaga gatgatggtc cgcgtccagg ctcgattgat cgaggttctc ggccccaagc
     1561 tctacgactc ttcgctgtcg gacgagcatc tcgaatcact tgtgctcggt cgggtgcagg
     1621 aactcctcga ggaagagagc tcgaccctca cgctgaccga gcgaaacatg ttgatgagga
     1681 cgatcgccga cggagtcctc ggactcggcc ccctcgagga cctcatgagg gatcccagtg
     1741 tcagcgagat actcgtgaat ggtcatgaca cgatcttcgt cgagcgtgag gggaagcttc
     1801 atcgcaccga cgcgcatttc ctcaacgaag agcaactccg ccacgtcatc gacaagaccg
     1861 ttgcgagggt cggacgccgg atcgacgagt cctcgccata tgtcgacgct cggctagacg
     1921 atggatcgcg agtcaatgcg atcatcccgc cgcttgccat cgacggcccc gtactgacga
     1981 tccgcaaatt ctcggccgaa ccctacgtct gggacgacct gatcaccttc ggcacaatga
     2041 cccccgaggt gaagaccctt ctcgaggcat gtgttcgggg ccgtttgaat atcttggtct
     2101 cgggtggaac cggttccggt aagacgacct cgctcaacgt gttgtcgtcg ttcatcccga
     2161 ccgatgagcg gatcatcacg atcgaggacg ccgccgagct cagactgcag caaccgcacg
     2221 tcgtacgact cgagtaccgg cctccgaaca tcgaaggcgc gggagagatc agcatccgcc
     2281 acctcgtgcg gaactcgctc cgcatgcggc ccgatcgcat cgtcgtgggc gaggtgcggg
     2341 gcaaagaagc cctcgacatg ttgcaagcga tgaacaccgg gcacgatggc tcgatctcca
     2401 ccatccacgc caactctcct cgtgatgccc ttgcgcgcct tgagacgatg tcgtcgatgg
     2461 cggggatgga tctgggtata cgggcgatcc gggaacagat gtcctccgcg atcgatctag
     2521 tgatccatca gtctcgactc aaggacggca gtcgacgatt cacccacgtg accgagatcg
     2581 tcggcatgga aggagaagtg ataaccctcc aggatctgtt cgttttcgac cactcggcag
     2641 ggatggacaa gaatggtcgt cacctcggtg cgctcaaacc gactgggctc cggcctgcct
     2701 tcctagacaa cctggaggaa cacgggatca ccatcccccc cgagaccttc gcggcggcgg
     2761 gagagaagtg gtgagaaagt tcgtagtcgt tgcgctcctg ctaaccgctc tcgcagtcgc
     2821 ggcccccgcc tcggctcaga ctggtgccct cactatccga aaggtcgata cagaggcctt
     2881 cccgaaagtg accgtcacgg ttgcttcgag tggtctggat gctttctcgg ccgagcagct
     2941 gttcgtccaa gagaacgggg cgcgcatcga catcctcgaa acggattcac tgatgtcctc
     3001 aggaaaacag atcgacgtcg tcctggtgat cgatacctcg ggcagcatgc tgggggatgc
     3061 gatggcagca gcaaaacgag ccgccgtgga cttcgtcaaa gctctcccgg atggggtgag
     3121 ggtcggcacc gtcaccttct ccgaccggcc ccacctcatc caggggataa ccaccgacca
     3181 ttcgtcggtg atccaaagga tacgcaccct ggaggcgacg ggagagaccg ccctgtacga
     3241 cggcgttgcg atggccgccg gcctgttcag cgggccgaac caacgcaacg tggttcttct
     3301 ctccgatgga ggcgacactg ccagcgcctc aacgctcaac gctgcggcac aagcgatcag
     3361 aaagagtgtc gcatcggtgt ttgccgtagg tctcgagacg acggaaaccg acgttgccgc
     3421 cctcaaacga ctcgccaacg tttcgagggg gagttatgcg tcggccgcca acgccgagct
     3481 ctccacgatc tatcagaacc ttgcaaccga gatccgcggc caattcgtcg ttacctacga
     3541 atcggctgcc ggctctgaag aggtggacct aacgatcggt atgccggggg cgatcgacga
     3601 agtaacgttc ctcgcccccg agcggatcgc cccaccgcga gacgtccgtc cttccgaacc
     3661 tgcgccgtcg cccgtgcggg gaccggccgg gatgatcctc tcgctcaccc tcttcttcct
     3721 cgccgtggca tcgatcatct tcatggcatc cggctcccga gcgcgtagga agaggaacga
     3781 ggagcttgct cgccgcttcg acgtaaaagg tccgacgccc gcatcgcgta ccggcgacca
     3841 ctcacaccgc aggttggtcc ccacccggct gggcaacgca gcaggaagag cagtacacgg
     3901 aagcagcatg gcgtcccgga tcgatcgcaa gctcgaacaa gccggattga aactgaccac
     3961 cggagaattc gtggtgggaa ccatcggtgc aggtctcgcg gcggcattga tcggggcaac
     4021 gctggccggg atcgcagcgg ctgtgctctt aggtgcggtt ggactcatcg gaccgttcct
     4081 atggctttcc cttagtgccc gaggacgaac gagccggatc aagggtcagc ttcccgacgt
     4141 cttgatgatc ctcgccagtt cactgcgagc cggtcacagc tttcttcaag cgctggacgc
     4201 ggtcgctcgg gagatcggag caccctcgga tcatgagttc aaccgtgcgg tcgccgagat
     4261 cagactcggt cgtccgatgg gagaaagcct caacgacctg gcggaccgca tcggaagtga
     4321 cgacttcaaa tgggccgtac ttgccatcgg catccagaaa gaagtgggag gcaacctggc
     4381 cgaggtgctc gacacggtcg cggcaaccct cagagaacgt gatgacgtcc gacggcaagt
     4441 acagacgctg tctgccgagg gcaggctctc cgcgtacatc ttgaccgctc tgcccttcgc
     4501 attgggcctc tatctacaga tgatcaatcc ggaagcggtc ggtctcctgt tctcgaccag
     4561 cctgggacgg atcatggtgg gcggaggaac caccctgcta ttactcggca ccctatggat
     4621 gagaaagatg gtgaagatcg atgtttgaca tcctgctagt gatggcgctt ggttccgctt
     4681 tcctagccgt ggccatcgtc gtcttggtcg tggattccac ctatgagaat cgcaagcgag
     4741 cggtcaagct cctggaggca gggataacca gctcggcggc ccccaccgcg aatctccgcg
     4801 agaaggaact caggaagtca ttcattcagc gggtgatcct gccagcgctc ggaagcttcg
     4861 gccgcttcgg ccgtcgcgtg acaccgttcg attcggctga tcggatcgaa atgcggttga
     4921 aagaagccgg cagccctcag gcttgggacg cggcccgcgt cgtggcagta aagctgatca
     4981 ccggcgcctt cggtctggtc gcttctttga ccttggcggc gactcaggag gccacgttcg
     5041 ctgcctcggt gctggtggtt ggactgggaa catgggggtc atacatgctt cccgaccttc
     5101 gcctgatgca gaaagcgcga gagcgacaga acgagatcag gaaggccatg cccgacacca
     5161 tggacctgct gacgatcatc gtggaagcag gtctcggttt cgatgcggcc ttggcgcatg
     5221 tcatccaaaa ggttccgggg ccgctcgcag atgagtacgg gaggacactg cacgagatcc
     5281 gactcggagt accgagggcc gatgccctgc gcaatatggc cgaccgaacc aacatcgagg
     5341 aggtcaggtc cttcgtcact tcgatggtgc aggcggagtc cttcggagtc agcatcacca
     5401 aggtgatcag agctcagtct cacgacctta ggtccaagcg ccgtgcgaat gcggagaaga
     5461 aggcccagca actggccgtg aagatgatct ttccgttgat cttctgcatc ctgccggcgg
     5521 tcctcgttgt tgcgatgggg ccgggagtga tccgaatcgt ccagacgttc ctctagggct
     5581 cagacgtcgg cgataagccg acgcacgcgt tcgaagcccg tttggatcgc ggtcgcggca
     5641 ccgaccgtat ccactagacg acgccactct tcaccgatct cttcagtcgc ggccatgcag
     5701 atcgccgccg cgacgcggtc ctctgcgtcg gcgtccgaac ggcccgcccg ctgcgcgaag
     5761 gcgtgcgcga tctttgcggt ccacttctcg aactcctcgg ctcggcgacc gttgagagtg
     5821 gggtcggtca acatcagctc gcgccgcaag atggctcggt ctctggttcc gtcctcctct
     5881 tccgccatgg ccgacacctc gatcagtgcc tcccggaacg cggcgagcgg accctccgtg
     5941 ccggggcgcc gaaccaggcg atcgaggaag cgttccccct gagtgtccgt gtcgtcgaag
     6001 aagacggcct ctttagatcc gtagtagcgg aagaaggtgc tccgcgaaac gtctgcggcg
     6061 gcggcgatct catctaccga cgtacgtgcg aagcccttct tcttgaagag gtcgagggcc
     6121 gcttcctcca aagcgcgtcg ggtagccgat ttcttcctct cgcgcaggtt cgtccgggaa
     6181 tctgcgcttt cggactggtc tagtgtcaag tgcgacgctc cccggtaggt ggtcccaacg
     6241 tcgaacaata ccgatactcc gtatcagttg gaacccgagt ctcagatgcg gagggagctt
     6301 gtccaccgat ttcttaccga tgtgggccgt aggcgtggcg gtcggcgccg ccgtgctggt
     6361 cgccgtcgga ctcgtgatct gggaccgtgc ctccaagcgc aaagaccgga agaacgggga
     6421 ctaatcgctg tcccccggct cgatcgggtg gatgtagagg tcgatggcca tccacaacga
     6481 cttcgagtac ggatagaaga agatcggtag caacccgttc gtgaccagag ccaccaccag
     6541 aagcggcccc caggcgacat cgggcatcga agcgagcagc accgccatta acaagacgaa
     6601 gaaccagatc tccgccactg cgatgttgac gatcaacgcc cccacccagt agccgctctc
     6661 acgttcgaag ttgtagccgc aatccgg
//
LOCUS       DATMED010000198         3773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5520381, whole genome shotgun sequence.
ACCESSION   DATMED010000198 DATMED010000000
VERSION     DATMED010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5520381"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VNP73_04980"
     CDS             complement(<1..338)
                     /locus_tag="VNP73_04980"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWL65309.1"
                     /translation="MLQKGDEAPDFEMTDQGGKTWSLGELRGQKFVLYFYPADDTPGC
                     TKESCDFRDSYSEWTDAGYQIFGVSPQGIESKKKFADKYSLPFPLLADEGTSVTKAYG
                     AFKDDGAEWDG"
     gene            complement(322..816)
                     /locus_tag="VNP73_04985"
     CDS             complement(322..816)
                     /locus_tag="VNP73_04985"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HWL65310.1"
                     /translation="MRTGDSIKLIFEEEEASLLVRLLTEMGTLVADDRQGPITERLFP
                     RTYEDEKDQAKYDELIGDQLRAVKLESIGKVRTALDGSEDGTVLIPPSDVDAWLAGLT
                     DTRVALGTRLQVTEEVMQKDLDPEDPEAPALSVLHWLGWVQESILKELNDAGKGGGPS
                     AAEG"
     gene            complement(827..1126)
                     /gene="clpS"
                     /locus_tag="VNP73_04990"
     CDS             complement(827..1126)
                     /gene="clpS"
                     /locus_tag="VNP73_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWL65311.1"
                     /translation="MAETLTAPTTTERTDTDDVTEPDRPWLVIVWNDPINLMSYVTWV
                     LQKLFGYSHDKATKLMLDVHQKGKAVVSSGAREKAELDCFRLHAHGLWATLSKSE"
     gene            complement(1119..2027)
                     /gene="gnd"
                     /locus_tag="VNP73_04995"
     CDS             complement(1119..2027)
                     /gene="gnd"
                     /locus_tag="VNP73_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWL65312.1"
                     /translation="MQIGMVGLGRMGGNMSLLLLGGGHEVVGFAPNPAEVERVVQAGG
                     HGTTSLGELVGALEAPRTVWLMVPAGAATQQTLTELADLLAPDDLVVDGGNSRYTDTI
                     ERAQMLAARDIHFADAGVSGGIWGLEEGYCLMVGCSRETFTRLEPVLATLAPADGYAH
                     VGPNGAGHFVKMVHNGIEYGLMQAYGEGFELLQSSDFDLDLAQVAEVWRRGSVVRSWL
                     LDLTARALNADPQLDAVAGFVEDSGEGRWTLHEAIDRAVPTPAIAMSLFARFASRQED
                     SFSARLLAALRKEFGGHAVQDRKIDG"
     gene            2090..2488
                     /locus_tag="VNP73_05000"
     CDS             2090..2488
                     /locus_tag="VNP73_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWL65313.1"
                     /translation="MKRWASLMLLAVLLVPFAGSTSAPAARVARKTHHVHAVGSWPMQ
                     FAWDPADIEIAAGDKVMWMNTTSGEHHITPYEGPWPDDVHLHLEGEDSKATFVFKKPG
                     VYKYYCDLQLHGQLLPGGVCAGQCGTITVR"
     gene            complement(2485..3288)
                     /locus_tag="VNP73_05005"
     CDS             complement(2485..3288)
                     /locus_tag="VNP73_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65314.1"
                     /translation="MAKRTATAVAPKPSLHRALKIVHRNAVSYRYYWTAIFTGFFEPL
                     FYLGAVGFGVGQFVGTVPVGGAQVDYATFLAPGMLAASTLNGAIADGFFFPFFKLNWL
                     KTYEGILATPLNVPDIAVGEMLWAQLRGTIYSAGFLVVMLALGLIESPWGILALPAAM
                     LSAGALSAGAMILTGLAKHITSLEKVMTLVVFPLFLFSGTFFPVELYPDALRPIVQVT
                     PLYHSASLLRSLTTGVVGVETLVNLGYLLVMYVAANLVAIRLMRKRLIA"
     gene            complement(3288..>3773)
                     /locus_tag="VNP73_05010"
     CDS             complement(3288..>3773)
                     /locus_tag="VNP73_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65315.1"
                     /translation="MLATPLQPRDVFLGELAWNGVMLTLQALPFLLIMGLFGITETPS
                     SLLAVPVAVLVGLAFFAAVMAWTATLQRDTAYNWLFRFVLTPLFLLSGTFFPIDPLPT
                     WAKVVANATPLYHGIELIRGFMISPITSAATAWHLFYLIVFLFIGTSLGIRNLTKRLT
                     P"
BASE COUNT          741 a         1181 c         1134 g          717 t
ORIGIN      
        1 ccatcccact cggccccatc gtctttgaac gccccgtatg cctttgtaac gctcgtgcct
       61 tcgtcggcca gaagggggaa cggcaacgag tatttgtcgg cgaacttctt cttggactcg
      121 atcccttgcg ggctcacccc gaagatctgg tagccggcgt ccgtccattc cgagtaggag
      181 tcccggaagt cgcacgactc tttggtacat ccgggcgtgt cgtcggccgg gtagaagtag
      241 aggacgaact tctgtccgcg gagttcgccg agactccacg tctttccacc ctggtcggtc
      301 atttcgaaat ccggtgcttc gtcacccttc tgcagcactc ggcccccctc ctttgcccgc
      361 atcgttcagt tctttgagta tcgattcttg cacccaaccc agccagtgga gaacggatag
      421 ggccggcgcc tcgggatctt ccggatcgag atccttctgc atgacttctt ccgtcacttg
      481 cagccgagtt cctagtgcca ccctggtatc ggttaatccg gccagccacg cgtccacgtc
      541 gctcggaggg atcagaaccg tgccgtcctc gctgccatcc agggccgtcc gtacctttcc
      601 gatgctctcc aatttcaccg cgcgaagttg atctccgatc agttcgtcgt acttggcctg
      661 gtccttctcg tcttcgtagg ttcgagggaa cagtcgttcc gtgatcggtc cttgtcggtc
      721 gtccgccacg agcgtgccca tctcagtaag gagtcgtacg aggagactcg cctcttcttc
      781 ttcgaagatc agcttgatcg agtcgcccgt gcgcatgcac tccacgtcat tcgctcttcg
      841 aaagagtggc ccacaatccg tgggcgtgta gacggaagca gtcgagctcg gccttctctc
      901 gagctccgct cgagacgacg gccttccctt tctgatggac gtcgagcatc agctttgttg
      961 ccttgtcgtg cgagtacccg aagagctttt ggagtaccca cgtgacgtag gacatgagat
     1021 tgatcggatc gttccagacg atcaccaacc agggacggtc gggctccgtg acatcgtcag
     1081 tatccgtccg ctcggtcgtc gtgggcgcgg tgagtgtctc agccatcgat ctttcgatct
     1141 tgcaccgcgt gtcctccgaa ctctttacgg agcgccgcga gcagccgtgc cgagaacgaa
     1201 tcctcctgtc gtgatgcaaa acgcgcgaac agtgacatcg cgatcgcggg cgtgggtacg
     1261 gcccgatcga tcgcctcatg cagtgtccag cgaccttcac ctgagtcttc gacgaaaccg
     1321 gcaaccgcgt ccagttgtgg atcggcgttc aatgctctgg cggtgagatc aagaagccac
     1381 gaacgaacga cgcttcctcg ccgccagacc tccgcgactt gagcgagatc gagatcgaag
     1441 tcgctgctct ggagaagctc gaacccctct ccgtatgcct gcatcaatcc gtactcgatc
     1501 ccgttgtgga ccatcttcac gaaatggccc gcgccgttcg gaccaacgtg tgcatagccg
     1561 tccgcggggg ccagggtggc gagaaccggc tcgaggcgcg tgaacgtttc gcgcgagcag
     1621 ccgaccatca agcagtaacc ctcttcgaga ccccagatcc ctccggacac acccgcgtcg
     1681 gcgaagtgga tatctcttgc cgccaacatc tgcgctcgtt cgatggtgtc ggtgtaacga
     1741 gagttgccgc cgtcgacgac tagatcatcg ggtgcaagca ggtccgcaag ctcggtcagc
     1801 gtctgttggg tcgcggcgcc ggccggcacc atgagccaga cggtgcgggg agcctcgaga
     1861 gcgccgacca gttcgcccag agaggtcgtg ccgtggccgc ctgcttggac cacgcgttcc
     1921 acctctgcgg ggttcggcgc aaagccgacc acctcgtggc caccgccgag caggagcaga
     1981 gacatgttcc cgcccatacg cccgagccca accatcccga tctgcatccg tggcaggtta
     2041 gcgccgagcc gggcaggaga tgccggtgcc gtggccgaat cctgagggca tgaagcgctg
     2101 ggcttcgctc atgctgctcg cagtcttgct cgtgcccttc gccgggagca catccgcccc
     2161 ggctgctcgg gttgctcgca agacccacca cgtgcatgcc gttgggagtt ggccgatgca
     2221 gttcgcgtgg gatcccgccg atatcgagat cgcggccggt gacaaggtga tgtggatgaa
     2281 caccacttcg ggcgagcatc acatcactcc ctacgagggg ccgtggcccg acgacgtgca
     2341 tctacatctc gagggcgagg actcgaaagc tacgttcgtc ttcaagaaac ccggcgtcta
     2401 caagtactac tgcgatctcc aattgcacgg ccagttgctt cccggcggag tatgcgccgg
     2461 ccaatgcggg acgatcaccg ttcgctaggc gatcaggcgt ttgcgcatca acctgatcgc
     2521 gaccagattc gccgcgacgt acatcacgag cagatagcca aggttcacaa gcgtctccac
     2581 accgaccacg cccgtcgtca ggcttcgtag cagcgacgcc gagtggtaaa gcggggttac
     2641 ttgcacgatg ggccgtagcg cgtcggggta gagctccacc ggaaagaaag tccccgagaa
     2701 gaggaacagc gggaagacga ccagcgtcat caccttttcg agggaagtga tgtgcttcgc
     2761 gagcccggtc aggatcatcg ctcccgccga gagcgcgccg gccgacaaca tcgctgcagg
     2821 cagggcgagg atgccccacg gggactcgat cagacccagg gccagcatca caacgaggaa
     2881 cccggccgag tagatggtgc ctctcagctg ggcccacaac atttctccca ccgcgatgtc
     2941 cggaacgttt aatggcgttg cgaggatgcc ttcgtatgtc ttcagccaat tcaatttgaa
     3001 gaacgggaag aagaatccat ccgcgatcgc gccgttcagc gtcgacgcgg caagcatgcc
     3061 gggggccagg aaggtcgcat agtcaacctg tgcaccaccc actggaaccg tgccgacgaa
     3121 ttgaccgacg ccgaacccga ctgcgccgag atagaagagc ggctcgaaga atcccgtaaa
     3181 gatcgcggtc cagtagtagc ggtagctcac cgcgttgcga tgaacgatct tcagcgcgcg
     3241 atgaagagac ggcttcggag cgacggcggt tgcggttcgc ttcgccatca cggagtgagc
     3301 ctcttagtca ggttccggat ccccagcgac gtcccgatga agaggaagac gatgagatag
     3361 aagaggtgcc aggcggttgc ggcagacgtg atcgggctga tcatgaaccc ccggatgagc
     3421 tcgatgccgt gatacagcgg cgttgcgttg gccaccacct tggcccaagt cggcaaggga
     3481 tcgatgggga agaacgtccc cgacagcaag aacagcgggg tcaggacgaa ccggaacagc
     3541 cagttgtagg ccgtgtccct ctgcagcgtc gcggtccagg ccatgaccgc tgcgaagaag
     3601 gcgagaccga cgagcaccgc gaccggaacc gccaggaggg atgaaggcgt ctcagtgatt
     3661 ccgaacagac ccatgatcaa gaggaacgga agtgcctgga gtgtcaacat cacgccattc
     3721 cacgcaagct ctcccaggaa gacgtcgcga ggttgtaagg gcgttgccag cat
//
LOCUS       DATMED010000199         7586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_531385, whole genome shotgun sequence.
ACCESSION   DATMED010000199 DATMED010000000
VERSION     DATMED010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531385"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VNP73_05015"
     CDS             complement(<1..628)
                     /locus_tag="VNP73_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWL65316.1"
                     /translation="MKALLIAGGFGTRMRPLTYTRPKHLLPVANRPHIQHVFDLLQRF
                     EINECVLLTSYLAEAFEETVRTAGERGMTLEVAHEEVPLGTAGALKNAQDLIGDGTFL
                     ALNGDILTDADLGELIDFHRVSKAEATILLTPVDDPSQFGVVPTDDAGRVTAFIEKPA
                     REEAPTNMINAGVYVIEPSVLDRIPTGRESSAERELFPSLVEDGVLFAR"
     gene            complement(631..1479)
                     /locus_tag="VNP73_05020"
     CDS             complement(631..1479)
                     /locus_tag="VNP73_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65317.1"
                     /translation="MSDRYSNLDAYLPPTPAERPNEPDPDAAEIAPRRRRLRLRRVGR
                     GLVGLLVAGLAASAVGAAAARVAVRGTSTVEARFNPAAVVPKERHEPRHAARLSLRDL
                     LSEPDRIDARRRPAKQRTPRRHTRRAALTSASVAAVPDAPSAPRPSKPERRRSSGDRA
                     NAPAKEEKQAPALPAPPGTPLFDCYSAKNNDHYTTNDRASAAQIETSKGGYTCTILGY
                     VYSYAAPGTRAIVLDYGTAHIFATRSAKTEPSCDKVALYRHSTGHDEWYSRTIADPGP
                     VGYIKP"
     gene            complement(1547..2011)
                     /locus_tag="VNP73_05025"
     CDS             complement(1547..2011)
                     /locus_tag="VNP73_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65318.1"
                     /translation="MALEVPPPEAIPDTTSFVARAVGGGRAVSLGLLVVSVIFAIAGQ
                     FTLKAAMNEIGRIGRSQVKTPVVTIAKAAKEPRLWLGLALFGISALFWLVVLSRVSLS
                     IAYPMVGLSYIVVVVVSKFLLHEAVPPLRWVGVTVIAIGIAVIGFSFRRATG"
     gene            complement(2058..3986)
                     /locus_tag="VNP73_05030"
     CDS             complement(2058..3986)
                     /locus_tag="VNP73_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HWL65319.1"
                     /translation="MRTIGRALVLVLLAVMLPALPARAQEATVQITGGGWGHGIGMSQ
                     YGAFGRAKRGDSAAQILEHYYSGAQVSEQKMPRRIRVGLLQYRKSISMSSSGSPGKVV
                     FKVAGSSDQVATGGAEASWRVEPSQTGGVRLYKDGEQIKEGGSGVLGDAAHPLVMLYE
                     PYGSTARIVEKARDYSLGRMEFGTYPSDRCDSFCLRLNVILAMQKYLYGLGEVPSSWP
                     QEALRSQAIAGRTYAYEKVLRSGQHREPCDCAVYDSTVDQAYVGDEKRTGSGSYWDDW
                     KSAVDATKKKVILYDGNPIQALYSSSSGGHTEHNENVWGGTPLPYLRGVRDNPDAVDE
                     NPNHTWSLEMSFKEFENKLNSAYHFGELEDFELVRPFGVSGRVTIPNGEKGGVRITGS
                     NETVRESGWSIRSALSLKDSLFRVQINYPVAEKFESRYRKLDGAPGRAKGPAYAVPRK
                     AKKTLGRAQNFRRGRMTWRRSTGKVVWQWGRVLRKYNKLGRERSELGMPTSDVWGPGF
                     YLGGTYSRGIIISARQTGNHTIRGGFKNIFQRNGGPQGPLGLPKENRRRVKSWPKGGL
                     RQRFTNGTIYLNPRTNRVFALWGAIDHRYRKMGAASSDCGYPVATLTSDKGVQKASFE
                     DGMIRAGAGGRVRVSCKG"
     gene            complement(4023..5078)
                     /locus_tag="VNP73_05035"
     CDS             complement(4023..5078)
                     /locus_tag="VNP73_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWL65320.1"
                     /translation="MRVALDAGPLLNAPTGVGRYTRELGIALEGIGVDVRRFAFAWRG
                     QAPAGVRRVKIPTRAAWSLWRRFGWPTAEGLVGGADVVHGTNFVLPASRRRGVVTIHD
                     LSYLQADAFPGAERLKELVPWSIKRAGAVVVPTEAIAAEVVDTYSVDRQTVHVTYEGV
                     SPVFFGATPLSDVALAGMGVSRPFVLATGTDAPRKNLQRLLDAWGATEAGAKGWTLVL
                     AGPSGWGPRLRRTEDIHPLGWVGDETLPGLMAAADIFCYPSLYEGFGLPPLEAMAAGT
                     ACLVGRYSAAPEVLGDAALVVDPTDADAIGEALTRLLADADLRKTLAIKGRGQATRYT
                     WANTAKATAEAYQAAMK"
     gene            complement(5081..6043)
                     /locus_tag="VNP73_05040"
     CDS             complement(5081..6043)
                     /locus_tag="VNP73_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWL65321.1"
                     /translation="MIESWANKKVLVTGGSGFLGSVVCRKLTERGAADIFVPRSRDYD
                     LTKPEGTEKLFADAKPDVVIHLAARVGGIGLNMERPADLYLANLLMGTYLIEEARRRQ
                     IEKTVIVGTICSYPKFTPVPFKEEDLWNGYPEETNAPYGIAKKALLVHAQSNRAQYGQ
                     RSIYLLPVNLYGPGDHFHPSVSHVIPALIKKCVDAKESGADRIEVWGTGKATREFLFV
                     EDAAEGIVTAVGRYDEAAPVNLGSGKEISIKELVELIASLVGFAGELVWDPSRPDGQP
                     RRSLDTSRAEREFGFVAQTTLEEGLGRTIEWYRAHREEAESLRD"
     gene            6109..7263
                     /locus_tag="VNP73_05045"
     CDS             6109..7263
                     /locus_tag="VNP73_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWL65322.1"
                     /translation="MGMKVGINLLWMVPGVVGGSETYTARLLHGLSERKADIEYTVFA
                     LPQLASAHPDLVTKFEVAFAPLTGQWKSFRVAGENSWLAAQTRKRGIDVIHHGGGIVP
                     IMPSSRPIVTIHDLQYLFYPEYFTRAKRTYLRRMVPRSAEAARLILTPSEYTRRTVIE
                     RLNIDPSVVVVVPHGISLRDESHEKADVRARFDIPGPFFLYPAAVYPHKNHLVLIDGF
                     ARLLKAHPDASLVLTGPKGWTEWKTATDMERRLTQEIDRLDLSARVKRLGYVSAKDLD
                     ALYREATALVFPSRFEGFGAPVLEAMSRGCPVLAADATATPEVVMDAGRLVSPDNSEE
                     WARGMSDLIEDEELRADLKKRGLERAREFEWTRSAGILEDCYRFVLETTL"
     gene            7260..>7586
                     /locus_tag="VNP73_05050"
     CDS             7260..>7586
                     /locus_tag="VNP73_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HWL65323.1"
                     /translation="MRLIVIPPHFEPDLAPTGVIFTRIVEELARRGHSVEVITSLPWY
                     REHQIEEGFGGRLVRHEDTPWGRITRLNPFPASDKRNIGRRALSFMGFSALATAVGMR
                     GDRVDAV"
BASE COUNT         1361 a         2490 c         2309 g         1426 t
ORIGIN      
        1 agcgggcgaa taggacccca tcttcgacca ggctcgggaa taactcgcgc tcggcggatg
       61 actcgcgccc tgtggggatg cgatccaaga cgctcggttc gatcacgtaa acgcccgcgt
      121 tgatcatgtt cgtcggcgcc tcctcgcggg ccggcttctc gatgaatgcc gttacgcggc
      181 cggcgtcgtc tgtcggaaca actccgaact gcgacggatc gtccaccggc gtcaagagga
      241 tcgtcgcctc ggctttgctg actcgatgga agtcgatcag ctctccgaga tcggcgtccg
      301 tgaggatgtc cccattcaat gcgaggaagg tgccgtctcc gatcagatcc tgtgcattct
      361 tcagagcacc cgcggttccg agaggcacct cctcatgagc gacctcgaga gtcatgcctc
      421 gttcaccagc ggtgcgaacc gtctcctcga aggcttcggc gaggtaggac gtcagtaaca
      481 cgcactcgtt gatctcgaac ctctgcagga gatcgaatac gtgttgtatg tgaggcctat
      541 ttgcgacggg cagtagatgt tttggtcgcg tgtaggtaag gggtcgcatc cgggtcccga
      601 acccgcccgc tatcagaaga gccttcatgg tcagggcttg atatacccca ccgggccggg
      661 gtcggcgatc gttctcgagt accactcgtc atgtccggtg ctatgacggt agagcgcaac
      721 cttgtcgcag gatggttccg tcttggcgct tctggtggcg aagatgtggg cggttccgta
      781 gtccagcacg atggctcgag tgcccggcgc ggcatacgaa tagacgtagc ccaagatcgt
      841 gcatgtgtaa ccacccttcg aagtctcgat ctgcgcagca ctcgcgcgat cgttcgtcgt
      901 gtagtggtcg ttgttcttcg cgctgtagca atcgaacaag ggggtccccg gcggagcagg
      961 gagagcggga gcttgcttct cttccttcgc gggcgcgttg gcccgatcgc ccgaagaccg
     1021 tctgcgctcg ggcttcgagg gacgaggcgc ggatggcgcg tccggaactg ccgcgacgga
     1081 ggcactcgtg agcgcggccc gacgggtgtg acgtcgcggt gtcctctgct tagccggcct
     1141 gcgccgggcg tcgatccggt cgggctcgga cagtaggtct cgtagcgaaa gcctcgccgc
     1201 gtggcgaggc tcgtgtcgtt ctttcggaac cactgcggcc gggttgaacc tcgcctcgac
     1261 ggtagaggtt cccctcacgg caactcgcgc cgcagccgca ccgacggcag aggcagcgag
     1321 cccggccacg agcaacccaa cgagccctcg accgacccgg cgcaacctga ggcgacggcg
     1381 acgcggagct atctccgctg catcagggtc gggttcgttc ggcctctccg ccggagtcgg
     1441 cgggaggtac gcatccagat tgctgtagcg atcggacatc gtggcctcga aggagcgcgg
     1501 gcttactccc gaggacttct ctagagccgg gtacgaatcc ttctgcttag cccgtcgctc
     1561 tgcggaaaga gaaaccgatc acggcgatac ctatcgcgat gaccgtgacc ccaacccatc
     1621 gaagaggtgg aacggcctcg tggaggagga atttcgaaac caccacgacg acgatgtacg
     1681 acaagccgac catcggatag gcgatcgaca ggctgacgcg cgacagcacg accagccaga
     1741 acagggccga gatcccgaac agggcaagcc ccagccagag gcgaggctcc ttggccgcct
     1801 tcgcgatcgt gaccaccggt gtcttcacct ggctgcgccc gatccggcct atctcgttca
     1861 tcgccgcctt gagggtgaat tgccccgcga tcgcgaagat cacgctcacc acgaggaggc
     1921 cgagggaaac ggcgcggccc ccacccacgg cgcgggccac aaaggaagtc gtgtcgggga
     1981 tcgcctctgg aggcggcact tcgagggcca tatggggcga ggctagcagg ggtgttcgtt
     2041 tatccggggc ggcttgttca acccttgcaa ctcacccgga cgcggccccc ggcacccgct
     2101 ctgatcatcc cgtcttcgaa ggacgctttc tgaacacctt tgtcggacgt caaggtcgcg
     2161 acgggatagc cgcaatcgga actcgccgct cccatctttc ggtacctgtg atcgatcgcg
     2221 ccccataacg cgaacacgcg gttcgtgcgc gggttcaggt agatcgtgcc gttggtgaat
     2281 cgctgacgga gtcccccctt cggccacgac ttgacgcgcc gacgattctc tttcggtagg
     2341 ccgagggggc cctgtggccc gccgttgcgc tggaagatgt tcttgaaacc gcctcggatc
     2401 gtgtgattac cggtctggcg cgccgagatg atgatccctc gtgaataggt cccgccgaga
     2461 tagaagccgg ggccccacac atcggaggtc ggcataccga gctccgagcg ttcgcgtccg
     2521 agcttgttgt atttgcgcaa gacgcggccc cactgccaca cgaccttgcc cgtcgaacga
     2581 cgccacgtca tgcggcccct gcggaagttc tgcgctcgcc ccaaggtctt cttcgctttg
     2641 cgaggcaccg cataggccgg gcctttagct ctgcccggcg caccgtcgag cttcctgtac
     2701 ctgctctcga acttctccgc taccgggtag ttgatctgca cacggaacaa cgagtccttc
     2761 aggctcaggg cgcttctgat cgaccatccg ctttcacgaa ccgtttcgtt cgaccccgtg
     2821 atacgtacgc cacccttttc cccattcggg atggtgaccc ggcccgacac accgaaggga
     2881 cggaccagct cgaagtcctc caactcgccg aagtggtaag cagagttgag cttgttctcg
     2941 aactccttga acgacatctc gagactccac gtgtggttcg ggttctcatc gaccgcatcc
     3001 gggttgtccc gcacacctcg gaggtacggc agaggcgtcc caccccacac gttctcgttg
     3061 tgttcggtat ggccgcccga ggatgaggaa tagagcgctt ggatcgggtt tccgtcgtag
     3121 aggatcactt tcttcttggt cgcgtccacg gccgacttcc aatcgtccca ataggagccg
     3181 gagccggtgc gtttctcgtc tccgacgtag gcctgatcca ccgtggagtc gtagacggcg
     3241 caatcgcacg gctcacgatg ctggccggac cggagaacct tctcgtaggc gtaggtgcga
     3301 ccggcgatcg cttgagaacg caaggcctct tgcggccacg acgaggggac ctcacccaaa
     3361 ccgtagaggt acttctgcat cgcgaggatc acgttcaacc gcagacagaa ggagtcacac
     3421 ctgtcgcttg gatacgttcc gaactccata cgaccgagcg aatagtcgcg ggctttctcg
     3481 acgatgcgag cggtcgagcc gtagggctcg tacagcatca ctaaggggtg agcggcgtcc
     3541 ccgagcaccc cgcttccgcc ttcctttatc tgttcgccat ccttgtagag acgaacgccg
     3601 cccgtctgac tgggctcgac cctccaagat gcttcggcac cccccgtcgc gacctgatcg
     3661 ctggagcccg caaccttgaa gacgaccttg ccgggtgagc cggacgagct catcgagatc
     3721 gacttgcggt attgaaggag gccgacgcgg atgcgacgtg gcatcttttg ttcgctgacc
     3781 tgagctcccg agtagtaatg ctcgaggatc tgcgccgccg aatcgcctcg cttggcgcgg
     3841 ccgaaagccc cgtattggga catgccgatc ccgtgccccc atcccccgcc ggtgatctgc
     3901 acggttgcct cttgtgcgcg cgccggtagg gccggcagca tcaccgccag caagaccaat
     3961 acgagcgccc gccctatcgt ccgcatccac accattctca tcgacaatgc ggcctacgtg
     4021 agctatttca tcgccgcttg gtatgcctcg gcggtcgcct tcgccgtatt cgcccacgtg
     4081 tagcgggttg cttgccctcg tcccttgatg gcaagggtct tgcgtagatc ggcgtcggcg
     4141 agaagccttg tcagggcttc tccgatggcg tcagcatcgg tcggatccac caccagcgcc
     4201 gcgtctccca aaacttccgg tgcggccgag tagcgcccga cgaggcacgc tgttccggcc
     4261 gccatcgctt ccaacggcgg taaaccgaaa ccttcgtaca acgacgggta acagaagatg
     4321 tccgcggcag ccatgaggcc cggtaacgtc tcgtcgccca cccatcccag cggatggatg
     4381 tcttccgttc tgcgcaacct gggaccccat ccactcggtc ctgcgaggac gagggtccaa
     4441 ccctttgccc ctgcctcggt tgccccccac gcatccaaca aacgctgcag gttcttcctc
     4501 ggcgcgtccg tgcccgtcgc cagcacgaaa ggtcgcgaga cgcccatccc cgcgagagca
     4561 acatccgaca gcggtgttgc cccgaagaag accggcgaca cgccttcgta tgtcacgtgc
     4621 accgtctgcc ggtccaccga gtaggtatcc acgacctccg ccgcgatcgc ttcggtggga
     4681 acaacgactg cccccgcgcg cttgatcgac cacggcacga gttccttcag tcgctccgca
     4741 ccggggaacg cgtcggcttg caggtaggac aggtcgtgga tcgtcacgac cccacgccgg
     4801 cgcgaggccg gcaggacgaa gttcgtaccg tgaacgacgt cggctccacc tacgagtccc
     4861 tccgccgtgg gccatccgaa gcgacgccac aacgaccacg ctgcgcgcgt cgggatcttg
     4921 acgcgcctga cccctgcggg tgcctgtccc ctccacgcga atgcgaaccg tcggacgtcg
     4981 acaccgatcc cctcgagcgc gatacccaac tcgcgcgtgt agcggccgac accggtgggc
     5041 gcattcagca gcggcccggc atcgagggcg acgcgcaagc tcagtccctc aggctttcgg
     5101 cttcctcgcg atgcgcccgg taccactcga tggttcgccc gagtccttcc tccagcgtcg
     5161 tctgcgcaac gaacccgaac tcgcgttcgg ctctcgaggt atcgagggag cgacgcggct
     5221 ggccatcggg ccgactcgga tcccacacca gctctcccgc gaacccgacg agggaggcga
     5281 tcaactccac aagctccttg atcgagatct ccttgccgga cccgagattc acgggagcgg
     5341 cttcgtcgta gcgcccgacc gccgtaacga tcccctcggc ggcgtcctcg acgaagagaa
     5401 actcccgcgt cgccttccct gttccccaca cctctatccg gtctgcccca gactccttcg
     5461 cgtcgacaca tttcttgatc agagccggga tcacgtggga cacggaggga tggaaatggt
     5521 ctccggggcc gtagagattc accggtaaga gatagatcga acgctgtccg tactgcgccc
     5581 ggttcgattg ggcatggacg aggagcgcct tcttggcgat gccgtagggg gcgttggtct
     5641 cttcggggta gccgttccag agatcctctt ccttgaacgg taccggagtg aacttggggt
     5701 acgaacagat cgtgccgacg atcacggtct tctcgatctg tcgccggcga gcttcctcga
     5761 tcagatacgt ccccatcaac aggttggcga gatagaggtc ggccggccgt tccatgttca
     5821 ggcctattcc tccaacgcgc gcagcgagat ggataaccac atccggcttg gcatccgcga
     5881 atagcttctc tgtgccttcg ggcttcgtta agtcgtagtc ccggctacgc ggcacgaaga
     5941 tgtctgcggc tccgcgttcg gtgagcttcc ggcagacgac actcccgagg aacccactgc
     6001 cccccgtgac caagaccttc ttgttcgccc acgactcgat catggagcca tgctaagcgt
     6061 gcatgagcca tctgaagggc tggtgcgcgc ctatcctgat ggccactgat gggcatgaaa
     6121 gttgggatca acctcttgtg gatggtgccc ggagtcgtcg ggggctccga gacctacacg
     6181 gcgcgacttc ttcacgggct aagcgagcgc aaggcggaca tcgagtacac cgtcttcgct
     6241 ctgccccaac tagcctccgc tcaccccgac ctcgtaacga agttcgaggt cgcctttgcc
     6301 cctctcacgg gacaatggaa gtcattccgc gtagcgggtg agaacagctg gctggcagcg
     6361 cagacccgta aacgcgggat cgacgtcatc caccacggag gcgggatcgt gccgataatg
     6421 ccgtcgtcgc gcccgatcgt cactatccac gacctgcaat atctgttcta tcccgagtac
     6481 ttcacgcgag cgaagcgcac ctacctccgg cggatggtcc cgcgctccgc cgaagccgca
     6541 cgactgattc tgacgcccag tgagtacacg cgcaggaccg tcatcgagcg actcaatatc
     6601 gatccgagtg tcgtcgtcgt ggttccacac gggatctcgt tgcgcgatga gagtcacgag
     6661 aaggcggacg tacgcgcgcg tttcgacatt ccgggtccgt tcttcctcta tccggccgcc
     6721 gtttacccac acaagaacca cctcgtcctg atagacggct ttgcgcggct gcttaaagcg
     6781 caccccgacg cgtccctggt ccttacgggt ccgaagggat ggaccgagtg gaagacggcg
     6841 accgacatgg aacgccggtt gacacaagag atcgatcgcc tggatctgtc tgcacgcgtc
     6901 aagaggttgg ggtacgtgtc ggcgaaggat ctcgatgctc tgtatcgaga ggcgacagcc
     6961 ctggtgttcc cgtcgcgctt cgaagggttc ggcgcgcccg tgctcgaggc gatgagccga
     7021 ggatgccccg tactcgccgc cgacgcgacg gcgacccccg aggtcgtgat ggatgccgga
     7081 agactcgtat caccggacaa ctcggaggaa tgggcccgag ggatgtcgga cctgatcgaa
     7141 gacgaagagc tgcgcgccga cctcaagaaa cgcgggctcg aacgagcccg cgagttcgag
     7201 tggacccgct cggcaggcat cctcgaggac tgttaccggt tcgtgctgga gaccacgctg
     7261 tgagactcat cgtcatcccg ccgcacttcg aacccgacct cgccccgacg ggagtcatct
     7321 tcactcggat cgtggaagag ctcgcgcggc gtgggcactc cgtggaggtc atcacttcgc
     7381 tcccctggta tcgggagcat cagatcgaag aagggttcgg ggggagactc gtgcgccacg
     7441 aggacacgcc gtggggacgg atcacgcgcc tgaatccttt ccccgcttcg gacaagcgca
     7501 atatcggccg cagagctctg tccttcatgg gcttcagcgc cctcgccacc gcggtcggga
     7561 tgcgtggaga ccgagtcgat gcagtc
//
LOCUS       DATMED010000200         4928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1307675, whole genome shotgun sequence.
ACCESSION   DATMED010000200 DATMED010000000
VERSION     DATMED010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307675"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..969
                     /locus_tag="VNP73_05055"
     CDS             106..969
                     /locus_tag="VNP73_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL65324.1"
                     /translation="MQLDEYKAHRKSVTTSKGEVAYAEFGDGPAAVFVHGVFMNGFVW
                     RNVIEGVKDIRRCIAIDLPAHGETKVEAENLGLKAHADLIAEVSDKLELGEIDLVGND
                     TGGAICQLFAVDQPDRLRSLTLTNCDVNENLPPEAFQGTVDLALAGELAPAISAIAND
                     INAARSEAGMGLGYEHPEKLSDEEVFAFLGYFREEEAGRSLERRIASIASHGNELVEI
                     EPKLRKLDVPTLIVWGTNDQFFGLEWAYWLKDRIPGVTEVVEIEGGKLFFIDERADEM
                     IPYLRKHWEAR"
     gene            966..1856
                     /locus_tag="VNP73_05060"
     CDS             966..1856
                     /locus_tag="VNP73_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL65325.1"
                     /translation="MSIDIKQGSITANGIEFGYLEAGNGPLALLLHGFPDNAWTWESQ
                     LDALASAGYHAVAPFLRGYPPTEVPKEHFDTETVSRDCCALIEALGSDNAYVVGHDWG
                     ALATLNAASVCPDRVKRAVAIGAGHPRTAIEIFKIPSQLHYAFHVWLFQLEGFGEFAL
                     KENDHALIDYLWDHWSEQSVDQAHVKRVKETFSHDGVAEATLSYYRGLVRIPSEKPDY
                     FALVTKNISVPTMVVYGAEDPAQVLSENESEFFSGEYRRELVPGAAHFVQREQPDALN
                     ALLLEWLSAPDRSVERASSA"
     gene            complement(1846..2496)
                     /locus_tag="VNP73_05065"
     CDS             complement(1846..2496)
                     /locus_tag="VNP73_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HWL65326.1"
                     /translation="MTRSTRRWAVGLPVILLGAAGLTAISMGAGGASSACLEKNQAKA
                     SLQDAEGNKVGRVVFGVHSTCATQMSASARGLTAGFHGLHVHTTGTCDPAATDPNGNP
                     SAFFTAGGHWSKETADHGSHTGDLPPLLVMEQGNTKAWSLTDRFQVRDLFDEDGSAVI
                     AHAGPDNLANVPAKTTTGADRYHSHAYDTFGPDEDTRKTGDAGARFACGVVQKIKR"
     gene            2600..3697
                     /locus_tag="VNP73_05070"
     CDS             2600..3697
                     /locus_tag="VNP73_05070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWL65327.1"
                     /translation="MHIEDAIESLEKENASLEPELLRLADAHRMLQLYARVQKLASFG
                     VAALARRIDDEQQVAKATGTSVGKAKETVATGKVMGSSAPLAAALQLGRLSLEQASEI
                     AKVEGSAPGTAKELLGVAEKEPFHVFKEKARKTRLEAEQNNGLGEKQHRARSARSYSD
                     ELGMVHVHLALEPHVGTPIVARAEAEAQRLARQAKGDKEPFECYLADAYAALLSGSGK
                     GRAKRPELVVLVSHEVAKRGWKEVRKGEVCKIPGVGPVPPQVAKRIAKDAFLSGLFFD
                     GVDLTKLKRWSRHIPVEVGIALELGEPPGFDGVACDDCGNRFRTEFDHVQPRVAGGPM
                     ANGNIKPRCWTCHRQKTERDRKAGKLTPPEP"
     gene            3789..>4928
                     /locus_tag="VNP73_05075"
     CDS             3789..>4928
                     /locus_tag="VNP73_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL65328.1"
                     /translation="MYPYRKAGPAAVLAAALVVAWMPAAQAAPTCFGRAATKVGTKGN
                     DTLKGTDGPDVIVSLDGRDDIESLGGADLVCGGEGGDNIRSGGGSDRVDGGGGPDSIN
                     GGGGNDRIGGGTSDDRLEGGSGKDKIVGATERYVKVVGKGDRKRRIVEQNYIEGGKGS
                     DDLTGGPNRDYLDGNEGDDRIAAGGDNDSLVGGEGDDRIDGGPARDEINYGNNKTSVS
                     LDLQTGRARGQGNDRLDNLEIVYGTRKDDVLRGDSADNELYGQTGSDKLDGRGGNDEL
                     IGYDGDDVLDGGPGRDLANYAYSYSGTVIVNLTADSATGDGNDTLRGMEDVIAGYGGD
                     TLIGNNSDNRLQGKGGNDTLRGLGGNDVLEGGREPVYDEATLEEAA"
BASE COUNT          989 a         1519 c         1629 g          791 t
ORIGIN      
        1 gtcgtctgct ccgtcaccga gcggagcgta gccggagccg attagacata cacacgatat
       61 ctcacatact atctgtatgt cagtcgggca cgacaaggag gcggcatgca actggacgag
      121 tacaaggcac atcgcaagag cgtgacgacg agcaagggtg aggtggccta tgccgaattc
      181 ggcgatgggc ccgcggccgt gttcgtgcac ggcgtgttca tgaacgggtt cgtatggcgc
      241 aacgtcatcg agggtgtgaa ggacatccgc aggtgcatag cgatcgacct ccccgcgcac
      301 ggcgagacga aggtcgaggc cgagaacctc gggttgaagg cacacgccga tctgatcgcc
      361 gaggtcagtg acaagttgga actcggcgag atcgatctgg tcgggaacga caccggcggc
      421 gcgatctgcc agctgttcgc cgtggatcaa cccgaccgtt tgcgatcgct gacgcttacg
      481 aactgcgacg tgaacgagaa tctgcccccc gaggcattcc agggaaccgt cgacctggcg
      541 ctcgcgggcg agttggcccc cgccatcagt gccatcgcga acgacatcaa cgctgcgcgg
      601 tccgaagcag ggatggggct gggttacgag caccccgaga agctcagcga cgaagaggta
      661 ttcgcttttc tcggctactt ccgagaagag gaggccgggc gttccttaga acgacgtatc
      721 gcttccatcg catctcacgg caacgaactc gtcgagatcg agcccaagct ccgcaagctc
      781 gacgtgccga ctctcatcgt ctggggaacc aacgaccagt tcttcgggct cgagtgggcg
      841 tactggttga aggatcggat ccccggcgtc acagaggttg tcgagatcga gggcggcaaa
      901 ctgttcttca tcgacgagcg agccgacgag atgatcccct acctccgcaa gcactgggag
      961 gcgcgatgag catcgacatc aagcagggca gcatcacggc taacgggatc gagttcgggt
     1021 acctggaagc gggcaacggc cccctcgcgt tgttgctcca cggtttcccc gacaacgcgt
     1081 ggacgtggga gtcgcaattg gatgctcttg cgtccgctgg ctaccacgca gtggctccct
     1141 tcttgcgcgg ctatcccccg actgaggtac ccaaggagca tttcgacacc gagaccgtgt
     1201 cgcgcgattg ctgtgcgctg atcgaagccc tgggcagcga caacgcctac gtggtcggtc
     1261 acgactgggg tgcactcgcg acgttgaacg cggcctcggt gtgccccgac cgggtgaaac
     1321 gggccgttgc gatcggtgcc ggtcacccgc gcacggcgat cgaaatcttc aagatcccga
     1381 gccagcttca ctacgcgttc cacgtgtggc tgttccagct cgagggcttc ggtgagttcg
     1441 ctctgaagga gaacgatcac gcgctgatcg attacttgtg ggaccactgg tcggaacaat
     1501 cggtggacca agctcacgtc aagcgcgtga aagaaacctt ttctcacgac ggcgtcgcgg
     1561 aggcgaccct ctcctactac cgagggctcg tcaggatccc ttcggagaaa cccgactact
     1621 tcgccctggt gacgaagaac atctcggttc cgacgatggt cgtctacggc gccgaagatc
     1681 ctgcacaggt cctgtccgag aacgagtccg aattcttctc gggcgagtac cggcgcgagc
     1741 ttgtgcccgg cgcggcccac ttcgtgcaac gagagcaacc ggacgcgctc aacgcattgc
     1801 tgctggagtg gctgagcgcg cccgatcgat cggtggagcg tgcctctagc gcttgatctt
     1861 ctggaccact ccacacgcga agcgggcccc ggcgtcaccc gtcttgcggg tgtcctcgtc
     1921 ggggccgaag gtgtcgtacg cgtgcgagtg gtaacgatcg gctccggttg tggtcttggc
     1981 cggcacgttc gccaagttgt cgggaccggc gtgcgcgatg accgcgctgc cgtcctcgtc
     2041 gaataagtcg cggacctgga acctgtcggt caacgaccac gcctttgtgt tgccttgctc
     2101 catcaccaac agcggcggaa ggtcacctgt gtggctgccg tgatcggccg tctccttact
     2161 ccaatggcct ccggccgtaa agaacgccga cgggttcccg ttcgggtcgg tcgccgccgg
     2221 atcgcaggtg ccggtcgtgt gtacgtgcag cccatggaag ccggcagtga ggccccgggc
     2281 cgaggcgctc atctgcgtcg cacacgttga atggacaccg aaaacgactc ggcccacctt
     2341 gttgccctct gcatcctgga ggctcgcctt ggcctggttc ttctccaggc aggcgctgga
     2401 cgctcccccg gcccccatcg atatagctgt gaggccggcc gctcccagca ggatcaccgg
     2461 gaggccaacg gcccatctgc gcgtggatct cgtcatcgat cgtcctctct ctctcggccg
     2521 atttagcaaa aaaagaaaaa tccggcagat cgtcccttac ccacccaaaa ctgtcacacc
     2581 ccctcgtcag gatgggggca tgcacatcga agacgccatc gaatcgctcg aaaaggagaa
     2641 cgcttccctc gagcccgagc tgctgcggct ggccgatgcc cacaggatgc tgcagctcta
     2701 cgcccgggtt cagaagctgg cctccttcgg cgtggccgcg ctggcgcggc ggatcgacga
     2761 cgaacagcag gtcgccaagg cgacggggac gtcggtcggg aaagccaagg aaaccgtggc
     2821 cacaggcaag gtgatgggct cgtctgcgcc cctggccgcg gctcttcagc tgggccggct
     2881 atcgctcgag caggcttcgg agatcgcaaa ggtcgaaggc tccgctcccg gcacggccaa
     2941 ggagctactt ggcgtagccg agaaagagcc cttccatgtg ttcaaggaga aggctcggaa
     3001 gaccaggctc gaggccgagc agaacaacgg cttgggggag aaacaacatc gggcgcgctc
     3061 ggcccgcagc tactcggacg agctgggcat ggtgcacgtg catctcgctc tagagccgca
     3121 tgtgggaacg ccgatagtgg cccgggccga ggccgaggcc cagcgactgg cgcggcaagc
     3181 caaaggcgac aaggaaccgt tcgagtgcta cttggccgac gcgtacgcgg cgttgctatc
     3241 cggctccggc aagggccggg ccaagcgacc ggagctggtc gtactggtca gccacgaagt
     3301 agcaaagagg ggatggaagg aagtccgcaa gggcgaggtg tgcaagatcc ccggggtggg
     3361 accggtgccg cctcaagtcg ccaagaggat cgctaaggac gcgttcctat ctgggctgtt
     3421 cttcgacggg gtcgacctga ccaagctcaa gcgatggtca cgccacatcc ccgtcgaggt
     3481 cgggatcgcc ttggagttgg gcgaaccgcc gggattcgac ggggtggcgt gcgacgactg
     3541 cggcaaccgg ttccggacag agttcgatca cgtgcagccg cgcgtcgcgg gcgggccgat
     3601 ggccaacggg aacatcaaac cgcgctgctg gacatgtcat cgacagaaga ccgagcggga
     3661 tcgcaaggcg ggcaagctga cgcccccaga gccgtgacca accagttcag gcgaggcaag
     3721 caatgaacgc tgagctggaa gcgtctcttg ggtatagaga gaaaaagcga tccccaggag
     3781 gtcagacctt gtatccctat agaaaagcgg ggccggccgc ggtcctcgcg gcagctctcg
     3841 tggtcgcgtg gatgcccgcg gcccaagccg caccaacctg cttcgggcgc gccgccacga
     3901 aggtgggcac caaaggcaac gacacgctca aaggaaccga tggccccgac gtgatcgtga
     3961 gcctcgacgg gcgcgacgac atcgagagcc tcggtggagc cgaccttgtc tgtggaggcg
     4021 agggcggcga caacatccgc tccggaggtg gctccgaccg cgtcgacggc gggggcggac
     4081 ccgactccat caatggtggt gggggcaacg accgcatcgg cggaggcacg agcgacgacc
     4141 ggctcgaagg tggttccggc aaggacaaga tcgtcggagc gaccgagcgc tacgtcaagg
     4201 tcgtcggcaa gggcgaccgg aaacgtcgca tcgttgagca gaactacatc gagggcggca
     4261 agggctctga cgatctgacc ggcgggccca accgcgacta cctcgacggc aacgagggtg
     4321 acgatcgcat cgcagcgggt ggagacaacg actcgttggt gggcggcgaa ggcgacgacc
     4381 ggatcgacgg tggacctgcc cgggacgaga tcaattacgg caacaacaag acgtccgtgt
     4441 ccctcgacct gcaaaccggc cgagcccgcg gacaaggtaa cgaccggtta gacaacctcg
     4501 agatcgtcta tgggacgcgc aaagacgacg tgttgcgagg cgactctgcc gacaacgagc
     4561 tctacggcca aaccggcagc gacaaacttg acggccgcgg cgggaatgac gaactcatcg
     4621 gctacgacgg agacgacgtt ctcgacggcg gccccggacg cgatctagcg aactacgcgt
     4681 actcctactc gggcactgtg atcgtgaacc tgactgcgga ttccgcaacg ggtgacggca
     4741 acgacacgct gcgcggcatg gaagacgtga tcgccggata cggcggcgac acgctcatcg
     4801 ggaacaactc ggacaaccgg ctgcaaggga aaggcggcaa cgacaccttg cgtgggttag
     4861 gcggcaacga cgttctcgaa ggcggccgcg agcccgtgta cgacgaagcc acgctcgagg
     4921 aggccgcc
//
LOCUS       DATMED010000201         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5522712, whole genome shotgun sequence.
ACCESSION   DATMED010000201 DATMED010000000
VERSION     DATMED010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5522712"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1277
                     /locus_tag="VNP73_05080"
     CDS             36..1277
                     /locus_tag="VNP73_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL65329.1"
                     /translation="MGTATEAITAPIPATHDRWHPSTAGWVARLTVLVLIFGGVLWVT
                     AIVPQYWADRISLAVIYCIIGLSLNVVLGYVGQVSLGHHAFVGIAAFVAAFYVTEQAG
                     CTAELGCPFDHFLAGIGLGVLSGGVAALLLGLVALRIRGLYLALITLTYGFMAERSIF
                     EIPSLTRGGAGMPAPRPDGILGLNFTSDRGFAFLTFVFLALVLFVDWRLLRSKVGRAI
                     LSIKHSEPVAASYGINVTAYKLLAFILSGMFAGLAGGLMAFRATTVVSNDFNFSIALL
                     WVLMVVVGGLGNRTGVVVISAFFALFPFLPELWTAADRFVVDTLERSMSLVTIVFGAL
                     LALLTIIQFPGGFAEQVSPITRWLRGQKFSMHPEGEGGHSKGHDKSKSDKGGGLLAKL
                     GLHRGEHDEPAEEADEPEGKN"
     gene            1277..2068
                     /locus_tag="VNP73_05085"
     CDS             1277..2068
                     /locus_tag="VNP73_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65330.1"
                     /translation="MSVLEIRDTGIRFGGLVALEGVNIDVPEWEIVGLIGPNGAGKTT
                     LFNCIMGLYTPNTGTVSYRERDISSLPTHRRVELGIGRTFQNIGLVRGLTVLENFLVA
                     QHPRIGYGSLSALAGFPRTFAEERRLKASAMEVLDFIGLTHLAESRLDGLPYGTLKLI
                     EIGTVLATDPDLLLLDEPTSGMGPEEAHEFGERLLELRKTLNLTVLMIEHHVPLVVGV
                     CDYVYVLNFGKVLTDGIPAEIKSHPEVIAAYLGGDVEEETTDVPA"
     gene            2055..>2651
                     /locus_tag="VNP73_05090"
     CDS             2055..>2651
                     /locus_tag="VNP73_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65331.1"
                     /translation="MSLLEVKDLRSGYGSLQVLQGISFSIEEGETAVLLGLNGAGKTT
                     TILSLAGLLKPWEGSVSFDGADVTGHDTRKLVSQGVVLVPEGRRIFPQLTVAQNLRLG
                     AWPYRKDRKRTREIVEQSVEYFPRLRERWTQLGGTLSGGEQQMLTLCRTLLGNPELIM
                     IDEPTEGLAPKIVELVGRYLQELKSRGISVLLIEQKLAI"
BASE COUNT          456 a          857 c          807 g          531 t
ORIGIN      
        1 ggccgcaggg cctgttgggg aaagaggcct aatccatggg gactgccacc gaggccatca
       61 ccgcaccgat accggcaacg cacgacaggt ggcatcccag caccgccgga tgggtcgctc
      121 gactaaccgt cctggtcctg atcttcggcg gcgttctgtg ggtaaccgcc atcgttccgc
      181 agtactgggc cgaccggatc tccttggcgg tcatctactg catcatcggg ctgtcgctga
      241 acgtcgtact cggatacgtc ggacaagttt cgctcggcca ccacgccttc gtcggtatcg
      301 ccgccttcgt agccgctttc tacgtaaccg agcaggccgg gtgcaccgcc gagctcggct
      361 gccccttcga ccacttcctc gccggcatcg gcctcggcgt tctctcgggc ggagtcgctg
      421 ctttgctcct cggcctcgta gcgctgcgga tccgtgggct ctacctggcc ctgatcaccc
      481 tgacctacgg attcatggcc gaacgctcga tcttcgagat cccgagcttg acacgcggtg
      541 gggccggcat gcccgctccc cgacccgacg ggatcctcgg tctgaacttc acgagcgatc
      601 gcggcttcgc gttcctcacg ttcgtgttcc tggcactcgt gttattcgtg gactggaggt
      661 tgctcagatc gaaggtgggt cgcgcgatcc tttcgatcaa gcactccgaa cccgttgcag
      721 cctcctacgg gatcaacgtc accgcttaca agctcctcgc cttcatcctg tcggggatgt
      781 tcgccgggct ggccggcggc ctgatggcgt ttcgcgccac gacggtcgtg tccaacgact
      841 tcaacttctc gatcgccctt ctgtgggtgc tgatggtcgt tgtgggcggg ctcgggaatc
      901 gcaccggtgt cgtcgtcatc tcggctttct tcgccctgtt cccgttcctt ccggagttgt
      961 ggacggcggc agatcgcttc gtcgttgaca ccctggaacg gtccatgtcc ttggtgacga
     1021 tcgttttcgg tgccctactg gctctactga cgatcatcca attccccggc gggttcgccg
     1081 agcaggtctc gcccatcact cgatggctgc ggggtcagaa gttctccatg catcccgaag
     1141 gagagggagg acactcaaaa ggccacgaca agtcgaagtc ggacaagggc ggtggcctac
     1201 tcgccaagct cggtttgcac cgaggcgagc acgacgaacc tgctgaagaa gcggacgagc
     1261 cggagggcaa gaactgatgt cagtactcga gatacgcgat acggggatcc gcttcggcgg
     1321 actcgtcgcg ctcgagggcg tgaacatcga cgttccggag tgggagatcg tcggtctcat
     1381 cgggcccaac ggagccggta agacgacgtt gttcaactgc atcatgggcc tttacacccc
     1441 gaacacgggg accgtctcct atcgcgagcg cgacatctcc tcgctgccga cccatcgccg
     1501 ggtcgagctc ggtatcggac gcacgttcca gaacatcggt ctcgtccggg gcctgaccgt
     1561 cctcgagaac ttcttggtcg ctcagcatcc gcgcatcggt tacggctcgc tctctgcact
     1621 tgccggcttc ccgcgcacct tcgccgagga gcgcaggctc aaggccagcg ctatggaggt
     1681 tctcgatttc atcggtctga ctcacctcgc cgaatcgcgc ctagatgggc tgccctacgg
     1741 gacgctcaaa ctgatcgaga tcggcaccgt gctggcgacc gaccccgatc tcctgctgtt
     1801 ggacgagcca acctcaggta tgggcccgga agaggcgcac gagttcggcg aacgtctgtt
     1861 ggagcttcgc aagacactca acctgaccgt gttgatgatc gagcaccacg tccccctcgt
     1921 cgtcggggtg tgcgactacg tctacgtctt gaacttcggg aaagtactga ccgatggcat
     1981 cccggccgag atcaagagcc accccgaggt catcgccgcg tatctcggcg gcgacgtcga
     2041 agaggagacc accgatgtcc ctgcttgagg tcaaggacct ccgctccggc tacggctccc
     2101 tccaggttct ccagggcatc tcgttctcga tcgaagaggg tgagacggcg gtactgctgg
     2161 ggctcaacgg tgccggcaaa acgaccacga tattgagcct cgccggcctt ctgaaaccgt
     2221 gggagggttc cgtgtcgttc gacggggccg acgttaccgg ccacgacacc cgcaagctcg
     2281 tttctcaggg cgtggtactg gtgcccgaag gcagacgcat ctttccgcag ttaacggtgg
     2341 cccagaacct gagattgggg gcatggccgt accgcaagga ccggaagcgc acccgcgaga
     2401 tcgtcgagca atcggtcgag tacttccccc ggctgcgcga gcgctggacc cagcttggcg
     2461 gaacgctttc cggtggcgaa cagcagatgc ttacgctgtg ccgaaccctg ctgggcaacc
     2521 cggaactgat catgatcgac gaaccgaccg aaggcctggc accgaagatc gtcgaactgg
     2581 tcggccgcta tcttcaggaa ctcaagtcgc gtggaatctc cgtgctgctg atcgagcaga
     2641 agctcgcgat c
//
LOCUS       DATMED010000202         4935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2088578, whole genome shotgun sequence.
ACCESSION   DATMED010000202 DATMED010000000
VERSION     DATMED010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088578"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..581)
                     /locus_tag="VNP73_05095"
     CDS             complement(30..581)
                     /locus_tag="VNP73_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65332.1"
                     /translation="MSARRIAIVGSFSTGKTTLAELMARELNLPLLPEVAREIAGLGF
                     KLDKDATPETEALIFLRQYNNELETPEFVGDRSMIDVMAYAGWVLDNQEYRKEMLIWE
                     ECIRLAERRLRTNYSHVFYLPIEFPIVVDELRPDDPDFQSEIDKRIVGLLETHDVAYE
                     TLTGSVEARLDRMHSHLGRAAAP"
     gene            complement(605..1498)
                     /locus_tag="VNP73_05100"
     CDS             complement(605..1498)
                     /locus_tag="VNP73_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWL65333.1"
                     /translation="MSKALRMSCVALVLALMTGCAFDDPPAADPGAPFVPEPAQTTVS
                     PDVGYHFAAIGDWGTGWEIEYTLARRMCELHDESAFEFVVTAGDNIYETGAPRDFDEK
                     FFQPMRCLHRRGVRFRAVLGNHDLLTRNGRPELNEPRFGMNGRNYVFRDGGMRFVMVD
                     SNRLRKKWLRQALAPEDGDIWTVVVFHHPVYSSSSGHESTGDFRFSLPDMFERRGVDL
                     VINGHTHVYAVTEPLHKIRYVTTGGGSASPHPCKDRWYTERCIVAYHFLSIVAGPDEI
                     KVVAIGRDGAPIDRFSTTGRD"
     gene            complement(1491..2120)
                     /locus_tag="VNP73_05105"
     CDS             complement(1491..2120)
                     /locus_tag="VNP73_05105"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWL65334.1"
                     /translation="MPSSQEGLARLIERSGLVDPRIADAFRRVDRAGFVPHDLIGEAY
                     LDRPVPIPHDQTTSQPSLIARMIDSAGIGPQDRVLEVGTGYGFQTALIAELARGVISI
                     ERFGSLAETARLNLRRSGLENALVKVGDGRSGAPEEGPYDAMIVSAASDDVPEALVEQ
                     LAEGGRLVIPVKSVGSDDVLLFRKTDGRLERVRLVTPARFVPLVRGSDE"
     gene            2156..3538
                     /locus_tag="VNP73_05110"
     CDS             2156..3538
                     /locus_tag="VNP73_05110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWL65335.1"
                     /translation="MTQPKRLRVLDEMFLHLEGPNTPMHVGAVAVYEGPPPDYHEFLD
                     EVWARLKNVPRFRQKLVTVPFGLGRPLWVDDTHFNAEYHLRHTALPPPGNYDKLRALA
                     SRIQSQQLDRSKPLWEMWFVEGLGADQWAVITKTHHAMVDGVSGADLFTVILDLTPEP
                     EQVEPERWVPDPEPTPDHLVIDALKERLISPRETMRTVRSALLDPNTLPNRVYEGARA
                     MGAFVGGSLDLAADSSLNQAVGPHRRFETVLADLDDFKMIKDSLGGSVNDVVLAVVSG
                     ALRTLLRSRGEQVDELELRAMVPVSVRADHDKGALGNQVASLYAPLPVHEEDPIKRFE
                     IVHEAMKDLKSSGQAVGAQLLTSLGEFAPPTILAQASRLVARQRAFNLVVTNVPGPQF
                     PLYSFGREMKEVYPVVPLSANTTICIALLSYNGKIGFGLLGDYDAAPDIGVLAESLEK
                     AIAELVSLSS"
     gene            complement(3528..3923)
                     /locus_tag="VNP73_05115"
     CDS             complement(3528..3923)
                     /locus_tag="VNP73_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HWL65336.1"
                     /translation="MSRRILQIVLAALGIVAVVAGAGQVLFGAAFTPHPGNFSANLDS
                     EMRFFAVWYVLIGILLVRASKDVESPGWTLRAVMATLFVAGCTRILSWATVGPPDTRF
                     VVLMVIELVLPLIVTPWQISVERAGEATN"
     gene            complement(3931..4410)
                     /locus_tag="VNP73_05120"
     CDS             complement(3931..4410)
                     /locus_tag="VNP73_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65337.1"
                     /translation="MLRRSVTALSLAAMLAACGNPSITPAAQDESPEDLGSISCTVSY
                     RPRPDAQGRESTLTVTPVRPPERHSDSERFGEMNVRIRYFDDGFDSPAFNVIVKSTGK
                     KEDIEARLYQFERTGDQQDAGPRRPENEFAGGHGFTGLTYVFDPDSPADVQYFCVAE"
     gene            complement(4445..>4935)
                     /locus_tag="VNP73_05125"
     CDS             complement(4445..>4935)
                     /locus_tag="VNP73_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HWL65338.1"
                     /translation="TDALVEVAGRVDAIVGAGTIVGPEQVKEAYDAGAEFAVTPVMVP
                     EIVDAAHELSMPIAVGASTPTEIFHAVQAGADWVKVFPAESLGGPDYVSHILGPLPGT
                     PILVSGGITDSNYLGYLDAGAQIIGFASAVFDPDIAAEGDYEELERRAIEVNRRLDMY
                     GK"
BASE COUNT          915 a         1582 c         1526 g          912 t
ORIGIN      
        1 cgacatcgtc gacgactatc tgaccaacgt cagggggccg cggcccggcc cagatgagaa
       61 tgcatccggt cgaggcgtgc ttcgacggat ccggtaaggg tctcgtaagc gacatcgtgg
      121 gtctcgagta ggcccacgat ccgcttgtcg atctcgctct ggaagtcggg gtcatcgggg
      181 cgtagttcat cgacgacgat cgggaactcg atcggcagat agaagacgtg cgagtaattg
      241 gtgcgaagcc ggcgctccgc caaccggatg cactcctccc agatcagcat ctccttgcgg
      301 tattcctggt tgtcgaggac ccatccggca taggccatga cgtcgatcat cgatcggtcc
      361 cccacgaact cgggcgtctc gagctcattg ttgtactgcc taaggaaaat gagtgcctct
      421 gtctcggggg tcgcgtcctt gtcgagcttg aagccgagtc ccgcgatctc tcgcgccacc
      481 tccggcaaca gtggaagatt caactcgcgt gccatcagtt cggcaagagt cgtcttgccc
      541 gttgagaagc ttccgacgat cgcgatcctt ctggcagaca tgagtgcgga tactagacgg
      601 ccactcagtc ccggcccgtc gtggagaaac gatcgatcgg cgccccatct ctgccgatgg
      661 ccacgacctt tatctcgtcc gggcccgcaa cgatgctgag gaagtgatag gcgacgatgc
      721 atcgctccgt gtaccaccgg tctttgcagg gatggggaga tgcgctgccc cctccggtcg
      781 tcacgtaccg gatcttgtgg aggggctccg ttactgcgta gacatgggtg tgaccgttga
      841 tcactagatc gactccgcgt ctctcgaaca tgtcgggaag tgagaagcgg aaatctcccg
      901 tcgactcgtg gcctgagctc gacgagtaga cagggtgatg gaaaacgacg accgtccaga
      961 tgtcgccgtc ctcgggtgcc agcgcttgac gcagccactt cttcctcaac cggttcgagt
     1021 cgaccatcac gaaccgcatg ccgccgtccc ggaaaacgta gttcctgcca ttcatcccga
     1081 aacgtggctc gttcagttcc ggtcggccgt ttcgcgtcag cagatcgtga ttgccgagaa
     1141 cggccctgaa gcgaacgccg cgccggtgga ggcaccgcat cggttggaag aacttctcgt
     1201 cgaagtcccg cggagcgccg gtctcgtaga tgttgtcccc cgccgtgacc acgaactcga
     1261 acgctgactc gtcatgcagt tcgcacatgc gtcgagcgag cgtgtactcg atctcccacc
     1321 ccgtccccca gtcgccgatc gcggcgaagt gatagccgac gtcgggcgag acggtcgtct
     1381 gagccggttc cggcacgaag ggtgcgcccg gatccgcggc gggcggatcg tcgaacgcac
     1441 aaccggtcat cagggcgagc acaagcgcaa cgcacgacat gcgtagtgct ttactcatcc
     1501 gaccccctca cgagcggaac gaaccgtgcc ggtgtcacga gtctgactcg ctccagccgt
     1561 ccatcggtct tgcggaacag taggacgtcg tcacttccca ccgatttgac ggggatgacc
     1621 aggcggcccc cctccgcgag ttgctcgacg agtgcttcgg gtacgtcgtc ggaggcggcc
     1681 gacacgatca tcgcgtcgta agggccttcc tcgggagcac cggaccgacc atccccgacc
     1741 tttacgagcg cgttctcgag accactccga cgcagattca gacgagcggt ctcggctagt
     1801 gacccgaatc gctcgatcga tatgacgcca cgggccagtt cggctatcag ggcggtttgg
     1861 aacccgtagc cggtgccgac ctcgagcacc cggtcctggg gaccgatccc cgccgaatcg
     1921 atcatccggg cgatcagcga aggctgactg gtcgtctggt cgtgcgggat gggaacgggc
     1981 cgatcgaggt acgcctcacc tatgagatcg tgcgggacaa aaccggctcg gtcgacacgt
     2041 cggaatgcgt cggcgatccg aggatcgacc agaccgctcc tctcgatcag ccgggccaac
     2101 ccctcctggc tgctcggcac gagcccacct taagtcccgc gctaccatca caggcatgac
     2161 ccagccgaag aggttgcgcg tgctcgatga gatgttcctt catctggaag ggccgaacac
     2221 cccgatgcat gtcggcgcgg tcgccgtcta cgaaggaccg ccgccggact atcacgaatt
     2281 ccttgacgag gtctgggcgc gcctgaagaa cgttcctcgc ttccgtcaga agcttgtaac
     2341 cgttcccttc gggctgggcc gaccactatg ggtcgacgac acgcacttca acgccgagta
     2401 ccaccttcgc cacaccgctc ttcccccgcc ggggaactac gacaagctcc gcgcactcgc
     2461 gtcgaggatc cagtcacagc aactcgaccg ttcgaagcca ctgtgggaga tgtggttcgt
     2521 cgagggtctg ggtgccgacc agtgggctgt gatcacgaag acgcaccacg cgatggtcga
     2581 cggtgtttcg ggggccgacc tcttcaccgt gatcctcgat ctgacgcccg agccggagca
     2641 ggtcgagcca gagcgctggg tgccggatcc ggagccgacg cccgaccatt tggtgatcga
     2701 cgcgctgaag gaacgtttga tctcgccgcg cgagacgatg cggacggttc gttctgcgct
     2761 gctcgatccg aacacgctcc ccaaccgcgt ctacgaggga gcacgcgcta tgggtgcgtt
     2821 cgtcggtggt tcgctcgatc tcgcggcgga ctcgtcgctt aaccaagccg tcggccctca
     2881 ccgcaggttc gaaaccgtcc ttgccgacct cgacgacttc aagatgatca aagactccct
     2941 cggaggaagc gtcaacgacg tggtccttgc cgtcgtatcg ggagcgctcc gcacccttct
     3001 acgaagccgc ggcgaacagg tggatgagct cgagcttcgg gcgatggtgc ccgtgtccgt
     3061 ccgagccgat catgacaagg gggcgctcgg caaccaggtc gcgtctctct acgcgccgct
     3121 gccggtgcac gaggaagatc ccatcaagcg gttcgaaatc gttcacgaag cgatgaagga
     3181 tctgaagtct tcggggcaag ccgttggtgc acaactgttg acatcgttgg gcgagttcgc
     3241 ccctccaacg atcctggccc aagcgtctcg tctcgttgcg cggcaacggg cgttcaacct
     3301 ggtcgtcacg aacgtcccgg ggcctcagtt ccccttgtat tcgttcggcc gagagatgaa
     3361 agaggtctat cccgtcgtcc ccctctcggc gaacaccacg atctgcatcg cactgttgtc
     3421 gtacaacggc aagatcggat tcggactgct gggcgattac gacgcggcgc ccgacatcgg
     3481 tgtgctcgcc gaaagcctcg agaaggcgat agcggagctc gtttctctta gttcgtagct
     3541 tcgccggcgc gctcgaccga tatctgccaa ggagtcacga tcagcggcag caccagctcg
     3601 atcaccatca acacaacgaa gcgggtgtcg ggcgggccga ccgtcgccca cgacaggatc
     3661 cgggtacaac ctgcgacgaa cagagtggcc atcacggcgc gcagcgtcca tcccggcgac
     3721 tcgacgtcct tcgatgctcg gacgagcaag atgccgatca ggacgtacca gacggcaaag
     3781 aagcgcatct cgctgtcgag gttcgcgctg aagttcccgg gatggggcgt gaaggcggcg
     3841 ccgaaaagca cctgtcccgc gccggcgacg accgcgacga tgccgagcgc cgcaagaacg
     3901 atctggagga tcctgcggct cacgtagttc ttactccgct acgcagaagt actggacgtc
     3961 cgccggcgaa tccgggtcga acacgtaggt caagcccgtg aacccgtgcc cacccgcgaa
     4021 ctcgttctcc ggacgtctcg gtccggcgtc ttgctgatct cccgttcgtt cgaattggta
     4081 gagacgcgcc tcgatgtctt ctttcttacc ggtggacttc acgatcacat tgaaggccgg
     4141 gctgtcgaaa ccgtcgtcga agtagcggat gcgaacgttc atctcgccga atctctccga
     4201 gtcactgtgt ctctcgggag gtctgaccgg ggtgacggtc agcgtcgatt cccgcccttg
     4261 agcatccggt cgaggccggt aggaaacggt gcatgagatc gaccccaagt cctccggact
     4321 ttcgtcttga gcggcggggg tgatggacgg attcccgcaa gcggccaaca tggccgctaa
     4381 ggagagtgcc gtcacggatc gtctcagcat gctcgttgga cggcgaggaa gagagcctgg
     4441 ttccctactt gccatacatg tcgagacggc ggttgacctc gatcgccctg cgctcgagct
     4501 cctcgtaatc gccttcggcc gcgatgtcgg gatcgaagac cgcgcttgcg aagccgatga
     4561 tctgcgcgcc ggcatcgagg tagccgaggt agttcgaatc ggtgatcccg ccgctgacca
     4621 ggatcggtgt ccccgggagg gggccgagta tgtgcgacac gtaatcggga ccgccgagcg
     4681 actcggccgg gaaaaccttg acccagtcgg ccccggcctg aacggcgtgg aatatctccg
     4741 tcggagtcga cgccccgacc gctatcggca tcgacaactc gtgggccgca tcgacgatct
     4801 cggggaccat gacgggagtg accgcgaact cggcgccggc atcgtaggcc tctttgacct
     4861 gctcggggcc gacgatcgtc ccggccccca cgatcgcgtc gacgcggccg gccacctcga
     4921 ctagagcatc cgtgg
//
LOCUS       DATMED010000203         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2474220, whole genome shotgun sequence.
ACCESSION   DATMED010000203 DATMED010000000
VERSION     DATMED010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474220"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..757
                     /locus_tag="VNP73_05130"
     CDS             251..757
                     /locus_tag="VNP73_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HWL65339.1"
                     /translation="MADLTVSVAEILGRPGAYRDFRVSGSLEGAGVALAQLEADSLAA
                     ELRAESVIEGVLITGRVQGTTVLTCARCLKDFRAPVEVEVCELFAGPGHEEDDPDAYR
                     VGGPDLHLEPMLRDVVTLALPLNPHCAEGCVGLCPRCGRELGNCSCEVEERDPRWAGL
                     DALRERLS"
     gene            773..949
                     /gene="rpmF"
                     /locus_tag="VNP73_05135"
     CDS             773..949
                     /gene="rpmF"
                     /locus_tag="VNP73_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWL65340.1"
                     /translation="MAVPKRRKSRSKMRMRRSHWKAEAPTYSACSQCREPKLPHRVCP
                     NCGYYAGRQAIETE"
     gene            972..1652
                     /gene="rnc"
                     /locus_tag="VNP73_05140"
     CDS             972..1652
                     /gene="rnc"
                     /locus_tag="VNP73_05140"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWL65341.1"
                     /translation="MELPTDPGPLYELALTHRSHAFENDSDEHNERLEFLGDAILGAV
                     VTDVIFNRYPDLSEGEMARLRASVVNTHALAEIAADLGLDERMRLGKGEEASGGRLKA
                     SLLANVFEAVVGAIYLDKGLAEVARRLTPIFDPPIEASMMSSSRYDAKTALQEVAVRR
                     TGELPDYRVASSGPDHDKRFVAHVFVDGEEFGSGSGRSKKEAEQHAATQALERFGEAE
                     GRGDARAS"
     gene            1636..2484
                     /gene="mutM"
                     /locus_tag="VNP73_05145"
     CDS             1636..2484
                     /gene="mutM"
                     /locus_tag="VNP73_05145"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWL65342.1"
                     /translation="MPELPEIEVLKRDLEKEVVGRRIKEAEVRPGSNAMRIVSRHGRR
                     KEFQELLEGAKVEKVERSGKLLLLELDSARVLVVDLGRTGILVKTSASEEVVTHTHIV
                     LAFTIGGQLRLIDTKHTAKVFVAPLDELQEIIESQPFMIDPLTHQFTWHYFSQLLEER
                     ASSMKELMSDEKFICGLGDIYSDEVLFAAGLRYDRSSDKLTSQDVRRLYRALMEILQD
                     AVKARGTTWGSAQYRDLQGEPGTFQLELKVYEREGEPCRRCRQDIVKEKWSGNRITYF
                     CPQCQT"
     gene            2503..2778
                     /locus_tag="VNP73_05150"
     CDS             2503..2778
                     /locus_tag="VNP73_05150"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007141703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWL65343.1"
                     /translation="MEDKTRVHVFVNGDVQGLGFRQAIADKANEQGVTGWVRNLRDGR
                     VEAVLEGPRDEVYRVVGLCRAGPKGARVDNVQVDREPPRNEKSFKIK"
     gene            complement(2771..>3224)
                     /locus_tag="VNP73_05155"
     CDS             complement(2771..>3224)
                     /locus_tag="VNP73_05155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWL65344.1"
                     /translation="AALLSGAGKGRPRRPELVVLVSHEVAKRGWKDVEKAEVCKIPGI
                     GPVSPQVAKTIAQDAFLSGVFYDGKDLRHFKRWTRSIPVEVGIALELGSAPGFDGVAC
                     IDCGNRFHNEFDHLEPYAAGGPTSNGNMKPRCWNCHQAKTKRERAGPI"
BASE COUNT          623 a          926 c         1072 g          603 t
ORIGIN      
        1 gcggccttcg agatcctgct gaacaagacc ctctcgaccg tcgcgcgcgg tcgcgagcaa
       61 ctgcgcggcg aggtccaggg cgagggggca ccgcaggcgg gtgcggacca gagcccgttc
      121 gatgcggagg agttcgacca ggcggccttc tagctttacc tggaagggcc tgggaaaaag
      181 gggggtcttg atatgattgg gcgtcccgcg tccgcgggtc cagttctcga gcagctgggg
      241 gcgttccttc gtggcagatc tgacggtatc ggttgccgag atactcggtc gtcccggcgc
      301 ttaccgggac ttcagggtgt cgggctctct cgagggggcc ggggttgcgc tcgcgcaact
      361 cgaagcagac tcgctcgcgg ccgagctgcg ggccgagagc gtgatcgaag gcgttctgat
      421 cacgggacgg gtgcaaggaa cgacggtgtt gacgtgcgct cgttgcttga aggactttcg
      481 ggcgccggtg gaagtcgagg tgtgcgagtt gttcgcggga ccgggacacg aggaggacga
      541 tccggatgcg taccgggtcg gcggtcccga tctgcacctc gagccgatgc tccgtgacgt
      601 tgtaacgctg gcactgccgc tcaatccgca ctgcgcggag ggatgcgtgg ggttgtgtcc
      661 gcgttgcggt cgcgagcttg gcaactgttc gtgtgaagtg gaagagcgcg acccgcgctg
      721 ggcggggctg gatgcgcttc gtgaacgatt gagctgatcg ggaggcaaga ggatggctgt
      781 tcccaagcga cgcaagtcgc ggtcgaagat gaggatgcgc cgctcgcatt ggaaggcgga
      841 agctcccacg tattcagcat gttcccagtg ccgcgagccc aagctcccgc accgtgtctg
      901 tcccaactgt ggttactacg ctggacgcca ggcgatcgaa acagagtagg tcggagcggt
      961 cggagccctc tttggagctt ccgaccgatc ccggcccgct ctacgaactt gccctcactc
     1021 atcgatcgca cgcgttcgaa aatgattccg acgagcacaa cgaacggttg gagttcctcg
     1081 gtgacgcgat cctcggggcc gtagtgacag atgtcatctt caatcgatat cccgacctca
     1141 gtgaaggaga gatggctcga ttgcgcgcct ccgtcgtcaa cacacatgcc ctggccgaga
     1201 tcgcagcaga tctcggattg gacgagcgga tgcgattggg caaaggcgag gaggcgtcgg
     1261 gcggccggct caaggcctcg ctgctggcga acgtgttcga agcggtcgtc ggcgcgatct
     1321 acctggataa aggactcgcc gaggtggccc gccggctcac acccatcttc gatcctccga
     1381 tcgaggcctc gatgatgtcg agctcgcgct acgacgcgaa aacggccctc caagaggttg
     1441 cggtccgacg gaccggcgag ttgcccgact accgagttgc gtcctccggc ccggaccacg
     1501 acaagaggtt cgtcgcgcac gtatttgtgg acggtgaaga gttcggtagc ggaagcggcc
     1561 gtagcaagaa agaggccgaa cagcatgctg ccacccaagc actcgaacgc ttcggtgagg
     1621 ctgaagggcg gggggatgcc agagcttcct gagatcgaag tactcaaacg ggatctcgag
     1681 aaagaggtcg tcggtcgccg gatcaaagag gccgaggtcc gacccggcag caacgcgatg
     1741 aggatcgtga gccggcacgg ccgtcgcaaa gagttccagg agctactcga gggagccaag
     1801 gtcgagaagg tcgagcggtc gggcaagctt ctgctcctcg agctcgactc ggcgcgcgtt
     1861 ctcgtcgtcg acctcgggcg gacgggaatc cttgtcaaaa cgagcgcgtc cgaggaggtc
     1921 gtcactcaca cccacatcgt gctggctttc acgatcggtg ggcaattgcg gttgatcgac
     1981 acgaaacaca cggcaaaggt cttcgtagcg cccttagacg agctccagga gatcatcgag
     2041 tcacagccgt tcatgatcga tcccttgacg catcaattca cctggcacta cttctctcaa
     2101 ctgctcgaag agcgggcgtc ctcgatgaag gaattgatgt cggacgagaa gttcatctgc
     2161 ggcttgggag atatctactc ggacgaggtc ctgttcgcgg cgggccttcg ttacgatcgg
     2221 tcctccgaca agctgacctc tcaggacgtg cgacgtctct accgcgcgtt gatggagatc
     2281 ctgcaggacg cggtcaaagc acgtggaacg acgtggggat cggcccagta ccgcgatctt
     2341 cagggagagc ccgggacgtt ccagttggaa ttgaaggttt atgaacgaga gggtgagccg
     2401 tgtcgccgtt gccgccagga catagtcaaa gaaaagtgga gcggcaatcg catcacctat
     2461 ttctgccctc aatgtcagac ataagcaacg ggaggtcatg agttggagga caagactcgc
     2521 gttcacgttt tcgtgaacgg tgatgttcaa ggtcttgggt tccgtcaggc gatcgcggac
     2581 aaggcgaacg aacaaggcgt tacaggctgg gtccgcaatc tccgagacgg acgggtcgag
     2641 gccgtcctcg aaggcccacg cgacgaggtt taccgggtgg tggggctatg ccgtgccggc
     2701 ccgaaggggg cccgcgtcga caacgttcag gtggaccggg agcctccccg caacgagaag
     2761 agcttcaaga tcaaataggc cctgcgcgct cgcgtttggt cttggcttga tgacagttcc
     2821 agcagcgtgg cttcatgttg ccgttcgagg tgggtccacc cgccgcatag ggctccaagt
     2881 gatcgaattc gttatggaag cgattgccac agtcgatgca ggccactccg tcgaaacccg
     2941 gtgctgaacc aagctccaac gcgatgccca cttcgaccgg gatcgaacgc gtccagcgct
     3001 tgaagtgacg caggtccttg ccgtcgtaga acacccccga caagaacgcg tcttgggcga
     3061 tggtctttgc cacttgaggt gacaccgggc cgatgccggg gatcttgcac acctcggcct
     3121 tctctacgtc tttccagcct cgcttcgcaa cctcgtggct gacgagcact acgagctctg
     3181 gtcttcttgg cctgccctta cccgcgcccg acaggagagc tgcg
//
LOCUS       DATMED010000204         4067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2475786, whole genome shotgun sequence.
ACCESSION   DATMED010000204 DATMED010000000
VERSION     DATMED010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475786"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VNP73_05160"
     CDS             <1..1373
                     /locus_tag="VNP73_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HWL65345.1"
                     /translation="VDDDGDVYVGGQTDADDFPTTPGAFQTEQNGGYDGFLSKLASNG
                     DLVYATYFSGSGSDLINDVAVDDSGSALVTGIAFSSDFPTENAWQEEKVGDGEAFAAK
                     FDTDGENLVYSTYLGDDEWDIGNEIAVDDEGAAYVIGQTYSPAFPSAWPIQRDHGGGS
                     DAFVTRLTSDGEVDFSTFFGGDWHDEWGSIDVVGNGTAAIGGTTDANSSFPTTSDAFQ
                     TEPGGDYDSYVAVFDIGETEPVEYGSDPPDPTPSPDESPKPTESPSPSPTGSPTPGPE
                     PTSPLTIRADVDVVVFGHEIHLSGDVNIPAPCSPGQIDVVRRVLGTDTFKPVGSALTT
                     EGQWLLDVTATKTASYKAVVEATETCPGYSSRLVEVQVKAAIDIEKAGSCASAKTIMG
                     RVRPNHAGTNVLLQARRQGDWVAVDRARLNELSRFRLTRETCRSEHRVVWRSRSKLVA
                     GASRRL"
     gene            1385..2707
                     /locus_tag="VNP73_05165"
     CDS             1385..2707
                     /locus_tag="VNP73_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWL65346.1"
                     /translation="MDVETKYAKSADLYIAYQAIGDGPMDVVFAPGFVSHLENDWEEP
                     RLVHFLENLSRFCRLIRFDKRGTGLSDRAECPSLETRMDDVRAVMDDAGSERAALVGY
                     SEGGVMAALFAATFPERTSALVVYGTFAKFTWAEDYPWALSPQRIANANALIEKEWGN
                     AITLPFWAPSLVEDEAFKKWWSRFERTGASPGAARALLDMNTEIDIRHVLPSIHVPTL
                     VLHRTGDAVVPVEGARWMAEQIPDAKYVELEGGDHAPFVGDSEGLVGEIEEFLTGVRH
                     APEVDRILATVMFTDIVGSTERASAMGDHYWKQLLEDHHARIRKQLEVYRGREVDTAG
                     DGFLAAFDGPGRAIKCAQAAQQAVRELGVDIRAGLHTGECEVFGDKLSGVAVHIGSRV
                     MSKADGPEVMVSSTVKDLVAGSGIEFEDRGEHELKGVPGSWHLFSVRS"
     gene            2752..3705
                     /locus_tag="VNP73_05170"
     CDS             2752..3705
                     /locus_tag="VNP73_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWL65347.1"
                     /translation="MRRSLAGLLVATAVLTAGGIAVAQGRPETIPLPQGWQPEGIAAA
                     GTTFYAGSIPTGDIWRGDLVTGEGGVFIDAPDGRRAVGIHLAEGWLLLVAGGPTGQGY
                     VYDINNGSEVDSFQFTAENTFINDVTMTSTAAYFTDSVNPVLYRVPFDGSAFGEPEVL
                     PYSGDLVYQEGFNVNGIDTTPSGKWLVVVQSNTGGLFKVDPASGETHAIDLGGADVTG
                     GDGILYDSWRTKNDRRCRRAKGRPGCLWVVENRDNLLTLVRFGAGLNSGKILSQTTHE
                     SFDVPTTVARSGRYMALVNARFGNETPATADYWVTQFKRPR"
     gene            complement(3707..>4067)
                     /locus_tag="VNP73_05175"
     CDS             complement(3707..>4067)
                     /locus_tag="VNP73_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65348.1"
                     /translation="GMTPVMGGYPALPGIEWTFDVGPRTFVAEAVADTSGPAFYLFEL
                     KGGSFVQLATPSGTYNPDDGYASILVPLSEIGARRASVISGVGKKGTEDVDAHVHLGA
                     TTHYPDRMATTKDIVVP"
BASE COUNT          779 a         1214 c         1334 g          740 t
ORIGIN      
        1 acgtcgatga cgacggtgat gtttacgttg gcgggcagac cgacgccgac gatttcccaa
       61 cgactcccgg tgccttccag accgaacaga acggtggcta cgacggattc ctgagcaagc
      121 tggcctcaaa cggtgacctc gtctacgcga cctacttcag cgggtcggga tcggacctca
      181 tcaacgacgt cgcggtcgat gacagcggca gtgctctcgt tacgggcatc gcattctcaa
      241 gcgatttccc caccgagaac gcctggcagg aagaaaaggt gggcgacgga gaggccttcg
      301 ccgcgaagtt cgacaccgac ggcgagaacc tcgtctattc gacctatctc ggcgatgacg
      361 agtgggatat cggtaatgag atcgcggtcg acgacgaggg cgcggcctac gtgatcggtc
      421 agacctactc cccagctttc ccgagcgcgt ggccgatcca aagagatcac ggcgggggct
      481 cagacgcgtt cgtcacccgg ctgacctcgg acggagaagt tgatttctcc acgttcttcg
      541 ggggcgattg gcatgacgaa tggggcagca tcgacgttgt cggaaatggg acggcggcga
      601 tcggtgggac tacggacgcg aactcctcct tccccacgac gtccgatgct tttcagacgg
      661 aacccggtgg cgattacgac tcgtacgtag ccgtcttcga catcggggaa acggaacccg
      721 tcgagtacgg atccgatccg ccggacccga cgccttcacc ggatgagtcg ccaaaaccga
      781 cggaatctcc atctccttca cccaccggat caccgacacc ggggccggaa ccgacctctc
      841 cgctgacgat ccgggccgac gtagacgttg tggtcttcgg gcacgagatc cacctctccg
      901 gcgacgtgaa catcccggcc ccctgttcgc ccggccagat cgatgtggtg agacgcgtgc
      961 tcgggaccga caccttcaag ccggttggtt ccgcactcac gacggaaggt cagtggctgc
     1021 tggacgtgac ggcgacgaag accgcttctt acaaagccgt ggtcgaggcg accgagacgt
     1081 gtcccggcta ctcatctcgt ctggtcgagg tacaggttaa ggctgcgatc gatatcgaga
     1141 aggcgggttc atgcgcaagt gcgaagacga tcatggggcg ggttcggccc aaccacgccg
     1201 gaacgaacgt cctgctgcaa gcacgcaggc agggcgattg ggttgcggtc gatagagcgc
     1261 ggctgaacga gctatcgcgc ttccgtctca cacgtgagac atgtcgatcc gaacatcggg
     1321 tcgtgtggcg cagccgctcc aagctcgtgg caggagcctc ccgccggcta tagcgttggg
     1381 ctcgatggat gtcgagacca agtacgccaa gagcgccgat ctgtacatcg cttatcaggc
     1441 gatcggcgat ggacccatgg acgtcgtgtt cgctcccgga ttcgtgtccc atctcgagaa
     1501 cgattgggaa gaaccccggc tggttcattt cctggagaac ctgtccaggt tctgccggct
     1561 gatccgcttc gacaagcgcg gaacaggcct ctcggatcga gccgaatgcc catccctcga
     1621 gacgcgcatg gacgacgtgc gtgccgtgat ggacgatgcc ggctccgagc gggccgcact
     1681 ggtcggttac tccgaaggcg gcgtgatggc ggctctgttc gcggccacgt tcccagagcg
     1741 cacgtcagct ttggtggtgt acggaacgtt cgcgaagttc acctgggccg aggactatcc
     1801 atgggccctc agcccccaac ggatagccaa cgcgaacgct ctcatcgaga aggaatgggg
     1861 caacgcgatc accctgccgt tctgggcccc gagtctcgtc gaagacgagg ctttcaagaa
     1921 gtggtggtcg cgcttcgaac gaaccggcgc gagcccgggg gcggcgcgag cgctcctcga
     1981 catgaacacg gagatcgata tccgccacgt ccttccttcc atccacgttc cgacgctcgt
     2041 tctccatcgc acgggtgatg ccgtcgtccc ggtcgaggga gcgcgctgga tggccgaaca
     2101 gatcccggac gccaagtacg tcgagctaga gggtggcgat cacgcaccgt tcgtcggcga
     2161 ctcggaggga ctagtcggcg agatcgaaga gttcctgacc ggcgtccgtc acgcccccga
     2221 ggtcgatcgc atcctggcaa ccgtgatgtt caccgacatc gtcggttcga ccgagagagc
     2281 gtcggcgatg ggcgaccatt attggaagca actcctcgag gaccaccacg ctcggatccg
     2341 taagcaattg gaggtctatc ggggccgaga ggtggatacc gccggcgacg gcttccttgc
     2401 agctttcgac ggaccgggtc gggcgatcaa gtgtgcgcaa gcggcccagc aagccgttcg
     2461 ggaactgggc gtcgatatcc gggctgggct tcacaccggc gaatgcgagg ttttcggcga
     2521 caagctgagc ggggtggccg tgcacatcgg gtctcgggtg atgtccaagg cggatgggcc
     2581 ggaggtgatg gtctcgagca cagtgaaaga cctggtggcg gggtctggga tcgagtttga
     2641 agaccgaggc gagcacgagt taaagggggt ccccggttcg tggcatcttt tctctgtcag
     2701 gtcctgaccg agaggggaaa accgatgggg tcggtggttc gtagtacaca catgcgcaga
     2761 tctctagccg gactgctcgt tgctaccgcg gtgctcacgg cgggcggcat cgcagtggct
     2821 caagggagac cggaaacgat cccgctcccg caaggctggc agcccgaggg gatcgccgcg
     2881 gctgggacca ccttctatgc aggatcgatc cccaccggcg acatctggcg cggtgatctc
     2941 gtaactggtg agggcggggt cttcatcgat gctcccgacg ggcgccgggc agtggggatc
     3001 catctggccg aagggtggct gctgctggtg gcgggcgggc cgaccggaca gggctatgtc
     3061 tacgacataa acaacggatc agaggtggac agttttcaat tcaccgctga gaacacattc
     3121 atcaacgacg tgacgatgac ttctacggcc gcctatttca ccgattccgt gaacccggtt
     3181 ctgtatcgcg tccccttcga tgggagcgcg ttcggcgagc cggaagtcct tccgtacagc
     3241 ggcgatctcg tctaccagga gggattcaac gtcaacggga tcgacaccac tccatccggc
     3301 aaatggctcg tcgtggtgca gagcaacacc ggtggcctgt tcaaagtcga tcccgcgagc
     3361 ggcgagacgc acgcgatcga tctcggggga gcagatgtga cgggtgggga cgggatcctt
     3421 tacgactcgt ggcggaccaa gaacgaccga cgttgccggc gcgctaaggg tcgaccgggt
     3481 tgcctctggg tggttgagaa ccgggacaat ctgttgacgc tcgttagatt cggtgccgga
     3541 ttgaactcgg gcaagatcct gagtcagacg acacacgagt cgttcgatgt gccgacgacg
     3601 gttgcgcgtt cgggccgtta catggctctc gtcaacgcgc gcttcggaaa cgagacgccg
     3661 gcaactgcgg actactgggt gacgcagttc aaacgaccgc ggtgaatcag ggaacgacga
     3721 tgtccttcgt cgtcgccatc ctgtcggggt aatgcgtggt tgcgccgagg tgcacgtgtg
     3781 cgtcgacatc ctcggtgcct ttcttgccga cgccggagat gaccgaagcg cgccgcgccc
     3841 cgatctcgct cagggggacc aggatgctgg cgtacccatc gtctgggttg taagtgccgc
     3901 tgggggttgc cagctgaacg aaggaaccgc ctttcagctc gaacagatag aaggcgggcc
     3961 cactggtgtc ggcaaccgct tcggctacga acgttcgcgg cccgacgtcg aaagtccact
     4021 cgatcccggg aagagccggg taaccaccca tcaccggtgt catgccc
//
LOCUS       DATMED010000205         4658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_917737, whole genome shotgun sequence.
ACCESSION   DATMED010000205 DATMED010000000
VERSION     DATMED010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917737"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VNP73_05180"
     CDS             <1..292
                     /locus_tag="VNP73_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWL65349.1"
                     /translation="IMFREVLPNLVAPIIVYATLIIPSNIIFEAALSYLGMGLPQSVP
                     SWGRMLSDAQDVFESAWWMMVFPGAFLFLTTLAFNLLGDGLRDALDPRTGRN"
     gene            377..2260
                     /locus_tag="VNP73_05185"
     CDS             377..2260
                     /locus_tag="VNP73_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL65350.1"
                     /translation="MFLNSRGLRLVALMLGLLLITAACAGGGGGAEPTDEGGEVQKGG
                     TLIREAASFDFTSGLDPTGEYLGTTFGFFSQALVRTLMNYRHVDGEAGNEVLPDLAAE
                     EPEISENGLTYTIPIREGVKFAPPVDRDVVCDDVLYAFERIGTPSVGAQYGFYYENTI
                     EGLLDFKDGKADTISGITCIDDYTIEFKLTEPTGDFLFRLAMPAAGPIPREVGECFEK
                     STEYGRYMISSSSYMLEGTDELNIDDGCKGMKAISGYDPSKHVILVRNPSYDEATDNP
                     ELRSNNIDRFEYRLNTNEDDIFRKVESGDIQMAQTPPPQALRRFSTDPELKDQLHVNS
                     GDRVWYITMNLAVPPFDDIHVRKAANLVTDKEALQRAWGGPVQGDIATHIVPDAMFGG
                     DLEDYDPYATPDHAGDLEAAKEEMKQSKYDSDGDGVCDDPVCTDVLHFARNESPWTEM
                     VPVIESSFKKIGIELRSRELADAYPPIQTVSNLIPVASVPGWGKDYPDVYTFLGFLFD
                     GRNILAEGNTNYSLVGLTPERGGELAEEGEFQFPAQDILGQIPSIDADIDACQDVTET
                     SERVECWIAVDQKLMEEVVPWVPYLDANNITVTSDAVTKWEFDPFAGEASIVHMAVDP
                     SQQ"
     gene            2365..3327
                     /locus_tag="VNP73_05190"
     CDS             2365..3327
                     /locus_tag="VNP73_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65351.1"
                     /translation="MGRYIIRRLLYLFLVIIIITVLMFAIFFLMPPTDPALAFAGKQP
                     TPEILAEVRRSLGLDKPVHVQYLLFVKRLFLGDEFGWPGLGRSYVYRESVRDLLIERF
                     PASFQLAVGGAIIWLSLGIPIGILSALKRRSFADRAAMVFALIGVSAPIFWLGQIALF
                     VFWQKLGISPGTGYEPLSNFGEWLSHMWLPWIILAINFAAFYARMVRGNLLETMGEDY
                     IRTARAKGLTERRVIGKHGLRSSLTPIATMFGMDFAILVGQAVITESVFNIQGVGVLT
                     VEAANRGDLPVLLGLTVLIALVVSFMSLIVDVLYAYLDPRVRYS"
     gene            3354..4382
                     /locus_tag="VNP73_05195"
     CDS             3354..4382
                     /locus_tag="VNP73_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65352.1"
                     /translation="MPLGEELLDVRDLRVHFDTADGVVKAVDGVTFKVRRGETLGIVG
                     ESGSGKSVTCLTALGLTSLTSATVSGEILFKGRNLLKLEKEDLRQIRGKEMSMIFQDP
                     FACLHPLYRVGDQIVEAIHAHDDCSKDDAKKRTIELLDSVGIPRPAERFRDFPHQYSG
                     GMRQRAMIAMALVHNPDLLIADEPTTALDVTVQAQILELIDRLKVDYNVGVILITHDL
                     GVVSDVADSVMVMYAGRPMEIGTRDAVFAQPLHPYTWGLLESIPHVDAKGTMLVPIEG
                     SPPSMIRVPPGCAFHPRCPHKFEPCDKDVPALTDRGGGHPESCHLTNEEKKRLWVERE
                     ARRTKATA"
     gene            4379..>4658
                     /locus_tag="VNP73_05200"
     CDS             4379..>4658
                     /locus_tag="VNP73_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL65353.1"
                     /translation="MSQTTEAPSPSAGSAPRGDALITVENLTKHFPITQGIIFQKEIG
                     RVHAVEEISFSVMQGETLGLVGESGCGKSTTARLVTKLLEPTSGKITFE"
BASE COUNT          905 a         1354 c         1470 g          929 t
ORIGIN      
        1 gatcatgttt cgcgaggtgc tacccaatct cgtagctccg atcatcgtgt acgcgacgtt
       61 gatcatcccc tcgaacatca tcttcgaggc cgccctgtcg tatctgggga tgggtttgcc
      121 gcaaagcgtt ccatcgtggg gccgcatgtt gtcggacgca caggatgtat tcgagtcggc
      181 ctggtggatg atggtcttcc ccggagcatt cctgttcctc acaacactgg ctttcaatct
      241 tttaggtgac gggttacgcg acgcgctcga tccccgaaca ggacgcaatt gacgagcgcg
      301 ctgcggcgag caaaggaggt gctttcggga cctcggggca tttttccagc aacgtaacgc
      361 gatggaaggg gtgaggatgt ttcttaattc acgtgggctg cgccttgtag ctctcatgtt
      421 gggtctccta ctgatcaccg cggcttgtgc cggcggagga ggtggggctg agccgacgga
      481 tgaaggtggc gaagtacaga agggcgggac gctgatccgc gaggccgcct cgttcgactt
      541 caccagcggg ctcgatccca ccggtgagta cctcgggacc acgttcggct tctttagcca
      601 ggccttggtc cggacgctta tgaactaccg ccacgtcgat ggtgaggcgg gtaacgaggt
      661 gcttcccgac ctggccgccg aagaaccgga gatctccgaa aacggactga cctacacgat
      721 cccgatcagg gagggcgtga agttcgctcc gccggtcgat cgcgacgtcg tctgcgacga
      781 cgtgctctat gcgttcgagc ggatcgggac cccctcggtg ggtgcgcagt acgggttcta
      841 ttacgagaac acgatcgagg gcctgctcga cttcaaggac gggaaggccg acacgatctc
      901 gggcatcacc tgtatcgacg attacacgat cgagttcaag ttgaccgagc cgaccggtga
      961 cttcctgttc cgacttgcga tgccggcagc gggaccgatc ccgcgcgagg tcggcgagtg
     1021 cttcgagaag tcgacggagt acgggcgtta catgatctcg tcctcctcgt acatgctcga
     1081 gggaaccgac gagctcaaca tcgacgacgg ctgtaagggt atgaaggcca tctcgggcta
     1141 cgacccgtcc aagcacgtca tcctggttcg caacccgagc tacgacgagg cgacggacaa
     1201 ccccgagctg cgttcgaaca acatcgaccg cttcgagtat cggttgaaca cgaacgaaga
     1261 tgacatcttc cggaaggtcg agagcggtga catccagatg gctcagaccc cgccgccgca
     1321 ggctctgcgt cggttctcga cagaccccga gctgaaggac caacttcacg tcaactcggg
     1381 tgaccgcgtc tggtacatca cgatgaacct ggccgttccg ccgttcgacg acatccatgt
     1441 gcggaaagca gccaacctcg ttacggacaa agaggcgctg cagcgtgcgt gggggggccc
     1501 ggtgcaaggc gacatcgcca cgcacatcgt tcccgacgcg atgttcggcg gagacctcga
     1561 ggactacgac ccgtacgcta cgccggacca cgcgggcgac ctcgaagccg ctaaggaaga
     1621 gatgaagcag tcgaagtacg actcggatgg ggacggcgtt tgtgacgacc ccgtgtgtac
     1681 cgacgtcctt cacttcgctc gtaacgagtc gccgtggacc gagatggttc cggtgatcga
     1741 gtcttcgttc aagaagatcg ggatcgagct gcgctcgcgc gagcttgccg acgcgtatcc
     1801 gccgatccag accgtgtcca acctgatccc ggtcgcatcc gtgccgggtt ggggtaagga
     1861 ctaccccgac gtctacacgt tcctcggctt cttgttcgat ggacgcaaca tccttgccga
     1921 ggggaacacg aactactcgc tggtcgggct gacgcccgaa cgtggcggcg agctcgccga
     1981 ggaaggcgag ttccaattcc cggcccagga catcctggga cagatcccga gcatcgatgc
     2041 agacatcgac gcgtgccagg acgtgaccga gaccagcgag cgggtcgaat gctggatcgc
     2101 cgtggaccag aagctcatgg aggaggtagt gccgtgggtg ccgtatctgg acgccaacaa
     2161 catcacggtg acttccgacg cagttacgaa atgggagttc gatcccttcg ccggtgaggc
     2221 ttcgatcgtg cacatggcgg tcgacccctc gcagcagtag cgagaggagt acgagtaacg
     2281 agtaggttcg agagccgccc tgcggggcgg ctctcgttca cctcagaaaa ccggatggac
     2341 cgagaggaag gtcgggggat ctagatgggt cgctacatca tcaggcgtct tctgtacctg
     2401 ttcttggtca tcatcatcat cacggttttg atgttcgcga tcttctttct gatgcctccg
     2461 acggatcccg cacttgcctt cgcggggaag caaccaacgc cagagatcct ggctgaagtg
     2521 agacgaagtc tgggcttgga caagccggtt cacgtccagt acctgctgtt cgtcaagcgg
     2581 ctgttcctcg gcgacgagtt cggatggccg ggattgggtc gttcctacgt ctaccgcgaa
     2641 tcggtccggg acctattgat cgagcggttc ccggccagct tccagcttgc ggttgggggt
     2701 gccatcatct ggctgtcgct cgggatcccg atcgggatcc tgtcggcgct caagagacgg
     2761 agctttgccg accgcgctgc gatggtgttc gcattgatag gcgtttccgc accaatcttt
     2821 tggctcgggc agatcgccct gttcgtcttc tggcagaagc ttggtatctc tccggggacg
     2881 ggctacgagc cgttatccaa cttcggggag tggttgagcc atatgtggct gccgtggatc
     2941 atcctggcca tcaacttcgc cgcgttctat gcccggatgg ttcgaggcaa cctcttggag
     3001 accatgggtg aggactacat caggacggcg cgcgccaagg ggctaaccga gcgcagggtc
     3061 atcgggaaac atggcctgag gtcgagtctg acccccatag cgacgatgtt cgggatggac
     3121 tttgcgatcc tggtcgggca agcggtcatc acagagagcg tctttaacat ccaaggagta
     3181 ggggtgctta cggtggaggc ggccaaccga ggcgaccttc ccgtcctgct cgggcttaca
     3241 gtactgatcg ccctggtcgt tagtttcatg tcgctcatag tggatgtgct ctatgcgtac
     3301 ctcgatcctc gggtcaggta ctcctagggt ggtcatccga aggagatagc aacgtgcctt
     3361 tgggggagga actcctagac gttcgcgatc tgcgcgtcca tttcgacacc gccgacggcg
     3421 tcgtcaaggc ggtcgatgga gtcaccttca aggtgcgacg tggggaaacg ctggggatcg
     3481 tgggcgagtc gggttccggc aagagcgtca cttgtctcac ggcgttggga ttgacgagcc
     3541 ttaccagcgc gaccgtcagc ggcgagatcc tcttcaaagg tcgcaacctc ctgaagctcg
     3601 agaaagagga cctgcgccag atccgcggca aagagatgtc gatgatcttc caggatccgt
     3661 tcgcctgcct gcatcctctg tatcgggtag gcgatcagat cgttgaggcg atccatgctc
     3721 acgacgattg ctcgaaggac gacgcgaaga agagaacgat cgaactcctc gattcggtcg
     3781 gtatcccccg gccggcagag cgattcagag atttccccca tcagtactcg ggtgggatgc
     3841 gacagcgcgc catgatcgcg atggcgctcg tccacaatcc cgatcttctg atcgccgacg
     3901 agccgaccac ggcgctagac gtcacggttc aggctcagat cttggaactg atcgaccggt
     3961 tgaaggttga ctacaacgtc ggcgtcatct tgatcacgca cgatctgggc gtggtgtccg
     4021 acgtcgccga ttcggtgatg gtgatgtatg cggggcgccc catggagatc ggtacgcgcg
     4081 acgcggtgtt cgctcaaccc cttcatccct acacctgggg tctactcgag tcgataccgc
     4141 acgttgacgc gaagggaacc atgcttgtcc cgatcgaggg atcgcctccg tcgatgatcc
     4201 gggttccccc tgggtgtgca ttccatccgc gctgtccgca caagttcgag ccctgcgaca
     4261 aggatgtgcc tgctctgaca gaccgcggcg gcggtcatcc ggaatcgtgt cacctcacga
     4321 acgaggagaa gaagaggctg tgggtcgagc gcgaagcgcg tcgtacgaag gccaccgcat
     4381 gagtcaaacc acagaagccc catcgccgtc ggccggatct gctccgcgcg gcgacgcgtt
     4441 gatcacggtg gagaacctca cgaagcactt cccgatcacg caggggatca tcttccagaa
     4501 ggagatcggg cgggtccacg cggtcgaaga aatctcgttc tccgtgatgc aaggcgagac
     4561 ccttgggctc gtaggcgaat ccgggtgtgg gaagtcgacg acggcaaggc tcgtcacgaa
     4621 gcttctcgag ccaacctccg ggaagatcac cttcgagg
//
LOCUS       DATMED010000206         4822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4376485, whole genome shotgun sequence.
ACCESSION   DATMED010000206 DATMED010000000
VERSION     DATMED010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4376485"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..821
                     /locus_tag="VNP73_05205"
     CDS             54..821
                     /locus_tag="VNP73_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005515537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWL65354.1"
                     /translation="MNVEGKVALVTGGASGLGLATVKELHGAGASVVILDLGNSDGET
                     VAKELGDNVRFAAADVTNEDEVGAAVELAASEFGGLNIAVNCAGIGWAERVVNRDGAA
                     ADLGRFEMVVRVNLIGTFNVIRLAAAQISAGEPDGDERGVIVNTASIAAFDGQIGQAA
                     YSASKGGVVGLTLPIARDLASRQIRCVTIAPGTFDTPMLAGLPEEARQKLAEGIPHPR
                     RLGKPEEFGLLVRQIVENPMLNGEVIRLDGALRMPPK"
     gene            858..1649
                     /locus_tag="VNP73_05210"
     CDS             858..1649
                     /locus_tag="VNP73_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWL65355.1"
                     /translation="MRHSSLFGPDSMMWRISRESVMLFGGRAAVLMQLAHPLVAAGVA
                     AHSDYRSDPYKRLRRTLDTMYTIVFESAEDARDAVARVNDVHDHVKGVAGDGRKYSAL
                     DPHLQLWVHTTLVYSSIKVYERFIEPLTPDELERYYQESKVIGSLFGIPGELHPETYE
                     ELVSWMWHLMDTGEVQVTDLARELADPIVRPLSLVPRGLSRGTSLITPGLLPRPIREG
                     YGFTVGASRAAVMAIGGRAARLVLPRLPQSVRTHPAARNAERRTA"
     gene            complement(1656..2072)
                     /locus_tag="VNP73_05215"
     CDS             complement(1656..2072)
                     /locus_tag="VNP73_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65356.1"
                     /translation="MSHPLPVHPTFASCACASQRLRCTLGTAPRTLSVKEFPLSTLDS
                     GYTPKQAERLQQIFERLVEARRELDALIGDAYLRPRASIVPVHFLDLAAAVEKLRWAE
                     KEWREIEETNARREDSQLEDPRVGVDELVSADSSSR"
     gene            2085..3467
                     /locus_tag="VNP73_05220"
     CDS             2085..3467
                     /locus_tag="VNP73_05220"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWL65357.1"
                     /translation="MLSEELVKKVLAAAAGKGSDLAELYVESKTSRSLRLEDRRIEDV
                     ASGKDAGAGVRVVAGPRSAYAFTNVLTEAALLETATIARAELAEDPGRKIDLRRVEPG
                     AVHPVERRAETASGEDLATILKASDETARAAGAEIRQVIVSYMSVEQNVLVASTDGRL
                     ATDDRSRLRFAVQVVAGRDGVITTGFDAPGHSGGLELLDRRPPAEIASRAATKALKML
                     DAKPSPAGEFPVVLAPGTGGVLIHEACGHGLEGDTLVKDASVYAKREGERFGSESVTI
                     IDDGSDVGAWGSFGVDDEGTPSQRTVLFDQGVLVGCMTDLNSAHKLNKNPTGNGRRQS
                     YAHLPVVRMTNTYLLPGPDDPDEIIRSIERGVYAATFAGGEVNPATGNFVFGMSEAYM
                     IENGAIAYPIKGANIIGNGPQVLQVIDAVAADFGRTEGVCGKDGQHAPVTNGMSTVLL
                     GKMTVGGTEA"
     gene            3467..4816
                     /locus_tag="VNP73_05225"
     CDS             3467..4816
                     /locus_tag="VNP73_05225"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWL65358.1"
                     /translation="MDLLEVAAGIVDRGRSGEALEAFVTHEREFHSKAFDGEIESISS
                     AEPRGAGVRTIVGDRAGFAYTTDLSTAGLDELVEQSRENASFASEDEAVALAPAWTDA
                     PGDIEGLFDDSQSELDVATKVAFAVELETATRAAHEKIRTVEEAVYADSSSQIAIATS
                     EGISSSYRRSDAWCYTLAIATDGDENEVGFEFDLARGLAGLDAEAVARRAASHAVSIL
                     GAGKIPSQKMPVVFDPYVAGQFLGVLGQALTGEAAQKGRSLFAGKLGEQVAATGLTLV
                     DDGRLAGAPGSSPWDDEGVPTQRTEVIGSGVLNSFLYDVTTARRESRESTGNASRAGF
                     KSAPGPAPSNMAFEPTGESVDELLKKAGTAFLVQDFHGVHSGANAVSGDFSVGATGRL
                     LKDGELGQPVKEVTIACPMLDILKGIVAVAPDRRWLPFGGSYGGSTTLIAEMTVAGS"
BASE COUNT          880 a         1448 c         1653 g          841 t
ORIGIN      
        1 tgcgaggatg gcctcatctt gtccacaggc gaggtcatca aggaggacat ttcttgaacg
       61 tcgagggaaa agtcgcgctc gtcaccgggg gcgcgtccgg actcgggttg gccacggtga
      121 aggagcttca tggcgcaggt gcctcggtcg tgatcctcga tctgggtaat tcggacggcg
      181 aaacggttgc gaaggaactc ggcgacaacg tcaggttcgc cgccgcggac gttacgaacg
      241 aggacgaagt cggggcagcc gtggagctcg ccgcgtcgga gttcggtgga ctgaacatcg
      301 ccgtgaactg tgcgggcatc ggatgggccg agcgcgtggt caatcgagac ggtgccgccg
      361 ctgatctcgg acgattcgag atggtagtgc gagtgaacct gatcgggacc ttcaatgtga
      421 tccgccttgc ggcagcgcag atctctgccg gagagcctga tggtgatgag cgtggcgtga
      481 tcgtgaacac ggcgagcatc gctgcattcg acggacagat cgggcaggcc gcttactcgg
      541 cttcgaaggg cggggtcgtg ggcctgaccc ttccgatcgc cagggacctc gcgagcagac
      601 agatcaggtg cgtgacgatc gcgccgggga cgttcgacac cccgatgttg gctggtctgc
      661 cagaagaagc gcgccagaag ctggccgaag ggatcccaca cccccgccgg ttgggcaagc
      721 cggaggagtt cgggctgctc gttcgccaga ttgtcgagaa cccgatgctg aacggtgaag
      781 tgatccgtct cgacggcgct ctccgcatgc ccccgaaata ggggccagcg gcccaccttc
      841 tgttgcacaa tgtagttgtg aggcacagct ccttgttcgg gccggactcg atgatgtggc
      901 gcatcagtcg cgagtccgtg atgttgttcg ggggccgggc ggcagtcctc atgcagctcg
      961 cacatccact ggtcgccgcc ggcgtcgccg cgcattccga ttaccgcagc gatccttaca
     1021 agcggctgcg ccggaccctc gacacgatgt acacgatcgt cttcgagtcg gccgaggacg
     1081 cccgcgacgc ggtggctagg gtcaacgacg ttcacgatca cgtgaaggga gtggccgggg
     1141 acggtcgcaa atactcggcc ctcgatcccc atctgcagct gtgggttcac acgactctcg
     1201 tctattcctc gatcaaggtg tacgagagat tcatcgagcc gctaacgccc gacgagctcg
     1261 agcgctacta ccaggagagc aaggtcatag gctcgctctt cggcatcccc ggcgaactac
     1321 accccgagac ctatgaggaa ctcgtctcgt ggatgtggca cctgatggac accggcgagg
     1381 tccaggtcac ggaccttgcc cgggaacttg ccgacccgat cgttcgccca ctcagcctcg
     1441 ttccgcgcgg gctctccaga ggaacctcgt tgatcactcc cggcctccta ccgcgtccga
     1501 tccgggaggg ttacggattc acggtggggg catcgcgcgc tgcagtgatg gcgatcgggg
     1561 gccgcgctgc gcggctggtg cttccgcgac taccgcaaag tgttcggacc catcccgcgg
     1621 ctagaaacgc cgaaaggcgg acggcctaga ggcggctagc gggacgaaga atcggcagac
     1681 accagctcgt cgacacccac cctagggtcc tcgagctggg agtcctcccg acgagcattg
     1741 gtctcttcga tctcgcgcca ttccttctca gcccagcgca acttctcgac ggccgcggcc
     1801 aggtcgagga agtgaacggg gacgatcgag gcacggggtc gcaggtaggc atcgccgata
     1861 agggcatcca gctcgcgcct cgcttctaca aggcgttcga agatctgctg gagccgctcg
     1921 gcctgcttag gggtgtagcc ggagtcgagc gtagacagag gaaactcctt tacagacagg
     1981 gttcttggag cggtgcccag ggtacatcga agccgctggg aggcacaagc gcacgaggca
     2041 aaagtggggt ggacggggag cggatgggac atatcctgcc gctcatgctg agtgaggagc
     2101 ttgtcaagaa ggtgctggcc gcggcggccg gcaagggctc cgatctggcc gagctctacg
     2161 tcgaatccaa gacttcccgg tccctccgtc tcgaggaccg ccggatcgag gacgttgcct
     2221 ctggaaagga tgcgggggcc ggtgtgcggg tcgtggccgg gcctcgatct gcctacgcct
     2281 tcacgaacgt tctgaccgag gccgcgcttc tcgagacggc cacgatcgcc cgggccgagc
     2341 tggccgagga cccgggtcgg aagatcgacc tgcgccgcgt ggagccgggg gccgttcatc
     2401 cggtcgagcg gcgggccgag acggcgtcgg gtgaggacct ggcgaccatc ctgaaagcct
     2461 ccgacgaaac cgcccgggcc gcgggggccg agatccgcca ggtgatcgtt tcttacatgt
     2521 cggtcgaaca aaacgtcttg gtggcttcaa cggacggacg gcttgcgacc gacgatcgct
     2581 cgcggcttcg gttcgcggtt caagtggtcg cggggcggga cggagtgatc actaccggct
     2641 tcgacgctcc cggtcattcc ggtggtcttg aactcctcga tcgtcgtccg cctgccgaga
     2701 ttgcatcgcg cgccgcgacg aaggcgctga agatgctcga cgcgaagcct tcacccgcag
     2761 gtgagtttcc cgtcgtgctc gcgcccggga ccgggggagt gttgatccac gaggcgtgcg
     2821 gacatggact cgaaggcgac acgctcgtca aggatgcgag cgtctatgcg aaacgcgagg
     2881 gcgaacgctt cggaagtgag tcggtaacga tcatcgacga cggctccgat gtcggtgcgt
     2941 ggggctcgtt cggtgtcgac gacgaaggga ccccatcgca acgcaccgtt ctgttcgatc
     3001 aaggcgtcct cgtcggctgc atgaccgacc tcaactccgc gcacaagctg aacaagaacc
     3061 caacgggcaa cggtcggcgc cagtcctacg ctcaccttcc ggttgtccgg atgacgaata
     3121 cctatctgct tccgggtccc gacgaccccg acgagatcat caggtcgatc gagcgcggtg
     3181 tttacgcggc aacgttcgcg ggcggcgagg tgaatccggc aaccggcaac ttcgtgtttg
     3241 ggatgtcgga ggcctacatg atcgagaacg gcgcgatcgc gtatccgatc aagggtgcaa
     3301 acatcatcgg gaatggaccc caggtccttc aggtcatcga cgctgtggcg gcggacttcg
     3361 gtcgcacgga gggggtctgt gggaaagacg gtcagcacgc tccggtgacg aacggtatgt
     3421 cgacggtgct tctcggcaag atgaccgtcg gcgggacgga agcctgatgg atctgttgga
     3481 agtcgccgcc gggatcgtcg accgcggccg gtcgggagag gcgctcgaag ctttcgtgac
     3541 acacgagcgg gagttccact cgaaggcgtt cgacggtgag atcgagtcga tctcttccgc
     3601 cgagccacgc ggggccggtg ttcggacgat cgtcggcgac cgagccggct tcgcttacac
     3661 gacggatcta agcaccgcgg gactcgacga actggtggag cagtcccgcg agaacgcgag
     3721 tttcgccagc gaggacgaag ccgtcgcatt ggcccccgct tggacggacg cacctggtga
     3781 catcgagggc ctcttcgacg acagccagtc ggaactagac gttgccacca aagtggcgtt
     3841 cgcagtcgag ctcgagaccg ctacgcgcgc cgctcacgag aagatccgga cggtggaaga
     3901 ggccgtctac gcggattcgt ccagtcagat cgcgatcgcc acctcggagg ggatctcgag
     3961 ttcctaccgg cggagcgacg cgtggtgtta cacgctcgcg atagccacgg atggcgacga
     4021 gaacgaggtg ggtttcgaat tcgacctcgc gcgcgggctg gccggtctgg atgcagaggc
     4081 ggtagctcgg cgtgctgcct cgcatgcggt ctcgatcctg ggggccggga agatcccgag
     4141 tcagaagatg ccggtcgtgt tcgaccccta cgttgccggg cagttcctcg gagtgttggg
     4201 ccaggcccta acaggagaag cggcgcagaa aggcaggtcc ctctttgcgg gaaagctcgg
     4261 cgagcaggta gccgccaccg gattgactct cgtcgacgat gggcgtttgg ccggagcgcc
     4321 gggaagctct ccgtgggacg acgaaggggt gccgacgcag agaaccgagg tgatcggatc
     4381 cggcgtcctc aactccttcc tttacgacgt cactaccgct cgcagagaga gccgcgagtc
     4441 gaccggcaat gcgtcgcgcg ccggtttcaa gtcggcgccg ggcccggccc cctcgaacat
     4501 ggcattcgaa ccgaccggcg aatcggtcga cgaactctta aagaaggcgg ggaccgcatt
     4561 cctcgttcag gacttccacg gagtgcactc gggcgcgaac gcagtctcgg gtgatttctc
     4621 tgtcggagcg accggccgcc tgttaaagga tggtgagctc gggcagccgg tgaaagaagt
     4681 gacgatcgcg tgcccgatgc tcgacatcct caagggcatc gttgccgttg ctcccgatcg
     4741 gagatggctg cctttcggcg gttcgtacgg aggctcgaca acactcatcg cggagatgac
     4801 ggtcgcgggg agttagacga tc
//
LOCUS       DATMED010000207         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3234840, whole genome shotgun sequence.
ACCESSION   DATMED010000207 DATMED010000000
VERSION     DATMED010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3234840"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="VNP73_05230"
     CDS             complement(<1..1894)
                     /locus_tag="VNP73_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWL65359.1"
                     /translation="MSKAPLAFKRLLLGRPMASGELGHTLLPKVFALPVFASDALSSM
                     SYATQEVLLVLVVAGTGALALGVPISIAVATLLAIVIVSYRQIVRAYPGGGGAYAVAY
                     ENLGTYPGLIVAGALLLDYVLTVAVSITAGAEALVSAVSSLHDYRLSTTIGLIGLVTY
                     ANLRGAKESARVFAIPTYGFVLSIFVLIITGLIRCLDGCPQAESALLKPEAHQMLTAF
                     LVLKAFSAGTTALTGVEAIAEGVPVFRYPQSRNAATTLSILGVLSISMFIGVAWLADK
                     TNVVYIHGLEGQRTVIAQIANAVFGGGPMFYIVQVMTVLILILAANTAYTGFPVLGSI
                     LAHDRFMPRQLLNRGDRLVFSNGVVILAVFAVMLVVAFNADLNRLIQLYLVGVFLSFT
                     LAQFGTVVRWRRTREPGWQRAAAINAVGGVVTGVVLCVVVLTKFVDGAWIVITAMPFL
                     IFMMRSVNRHYVSVAEELESAERRPVDRRPGHQHMVILVQKVDAASARSVGIVRSIRP
                     NSVSAITFDPTLQSAWRRLAPDIAIETLPGRGNSADRVRSYLRARRQDLEPDDFLTLV
                     LPEILHRKGLIEIATRSHLHRLKASLLREPGIQVFDVPMLRDEIDHSVDEARERARNF
                     VIVLVSG"
     gene            complement(1905..2276)
                     /locus_tag="VNP73_05235"
     CDS             complement(1905..2276)
                     /locus_tag="VNP73_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HWL65360.1"
                     /translation="MPFLSSFFHNLTASPDEIRAENLRHWADSIPGAVPITDIKSRER
                     TKVAGVIQNIRIDPRSGGSIEATITDGRGQLVARWLGRSELAGILLGAGLVMEGVVGD
                     HSGELLMLNPEYELVPSPEHG"
     gene            2356..3018
                     /locus_tag="VNP73_05240"
     CDS             2356..3018
                     /locus_tag="VNP73_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWL65361.1"
                     /translation="MHIVIAGCGRVGSELATNLERLGHSVVIIDKTERAFERLKPDFS
                     GKKIVGFAFDRDVLEAAGINETDAFASVTSGDNSNILSARVAKEHYRVPAVVARIYDP
                     RRAQIYQRLGIQTVATVRWTTDQILRTLMPDEVPVEYTVDNGEVVVTAIAAPSEIVGK
                     KATDVDLEGRRKVVAVSRFGVPRVPEKDLALQDGDILHVSVVRGDLPTLTEALKKVGG
                     PE"
BASE COUNT          649 a          914 c         1006 g          532 t
ORIGIN      
        1 ccccggagac gaggacgatc acgaagttcc gagcgcgctc acgagcctcg tcgaccgaat
       61 gatcgatctc atccctcaac atcgggacgt cgaatacctg gatacccggc tctcgtagca
      121 agcttgcttt gaggcgatgg agatgcgagc gggtggcgat ctcgatcagc cctttgcgat
      181 gaaggatctc aggtagcacc agggtcaaga aatcgtccgg ctctagatcc tgcctccgcg
      241 ctctgaggta ggagcgcacg cggtcggccg aattcccgcg gccgggcaac gtttcgatgg
      301 cgatgtccgg cgcgagtcgc cgccatgcgg attgaagggt cgggtcgaac gtgatcgcgc
      361 tgacgctgtt gggcctgatc gatctcacga taccgaccga gcgggcggat gcggcatcta
      421 ccttttgaac gagtatgacc atgtgctggt ggcccggtcg acggtcgacc ggacggcgtt
      481 cggcggattc gagttcttcg gcgacgctca cgtagtgccg gttgaccgag cgcatcatga
      541 agatcaagaa gggcatggcg gttatgacga tccacgctcc gtcgacgaac ttcgtgagca
      601 cgacaacaca cagcacgaca cctgtgacga caccgccgac ggcattgata gctgcagcgc
      661 gctgccagcc gggttctcgg gttctccgcc accgaacgac ggttccgaat tgagccagcg
      721 tgaacgaaag gaacactcca accaggtaga gctggatgag ccggttgagg tcggcattga
      781 aggcaacgac gagcatgacc gcgaacacgg ccaggatgac gacaccgttg gagaacacca
      841 gtctgtcgcc gcggttcagg agctggcgag gcatgaaacg gtcgtgagcc aggatcgaac
      901 ccagcacggg gaaccctgtg tatgcagtgt tcgcagccag gatgaggatc agcaccgtca
      961 tcacctgcac gatgtagaac atggggccgc caccgaagac cgcgttcgcg atctgagcga
     1021 tcaccgtgcg ctgaccttca agaccgtgga tgtacacgac gttcgtcttg tccgcgagcc
     1081 atgccacccc tatgaacatc gagatggaga gcacacccag tatcgatagg gtcgttgcag
     1141 cgttcctcga ctgcgggtag cggaaaaccg ggactccttc ggcgatggcc tcgaccccgg
     1201 tcaacgccgt tgtaccggcc gaaaacgcct tcaaaacgag gaatgccgtc agcatctggt
     1261 gggcttcggg tttcagcaga gcgctttcgg cttgaggaca gccgtcgagg cagcggatca
     1321 atccggtgat gatcagtaca aagatcgaaa ggacgaagcc gtaggtcggg attgcaaaga
     1381 cgcgagccga ttccttggcg ccccgcaggt ttgcgtaggt cacgagcccg atcaagccga
     1441 tcgtggtcga caacctgtaa tcgtgaagac tgcttacggc ggacacgagc gcctcggcgc
     1501 cggcggtgat cgagacggca accgtgagca cgtagtccag gagtagagcg cctgcgacga
     1561 tgaggccggg ataggtcccc aggttctcgt aagcaaccgc gtacgcgcct ccccctccgg
     1621 gataggcgcg cacgatctgt cgataagaga cgatcactat cgccagcaag gtcgcgaccg
     1681 cgatcgaaat gggcacgccc agcgcgagtg caccggttcc cgcgacgaca agcacgagca
     1741 gtacctcttg ggtggcatag gacatggacg aaagagcgtc cgaggcgaag accggaagtg
     1801 cgaaaacctt cggcaacagt gtgtggccca gctcgcccga ggccatcgga cggccgagga
     1861 gcaggcgctt gaaggcgaga ggggctttgc tcatcgaagg aggctcaacc gtgctcgggg
     1921 ctggggacca gttcgtactc ggggttgagc atgaggagct cgccgctgtg gtcgccgacc
     1981 acgccctcca tgaccaaacc ggcaccgagg aggatgccgg ccagctccga tcggccgagc
     2041 cagcgggcca ccagctgccc cctgccatcg gtgatagtgg cttcgatcga tccgcctgaa
     2101 cggggatcga tgcggatgtt ctggatgacc ccggcaacct ttgtgcgctc acgcgacttt
     2161 atgtcggtga tgggaacggc gccgggtatc gagtccgccc agtggcgaag gttctctgct
     2221 ctgatctcgt ccggcgatgc cgtgaggttg tgaaagaagc tgctgaggaa cggcacgacg
     2281 gcaatctaac cacccaagcc cctcgctgga cgggtaaggc tgtggcaata tccgcggcgt
     2341 cgataaggag ttcgaatgca catcgtgata gccgggtgtg gccgagtggg atcggaactg
     2401 gcgaccaacc tcgaacgttt agggcactcc gtcgtgatca tcgacaagac tgagcgggcc
     2461 ttcgaaaggc tgaagccgga cttcagcggg aagaagatcg tcggtttcgc attcgatcgt
     2521 gacgttctcg aggcggccgg gatcaatgag accgacgcct tcgcttccgt aaccagcggg
     2581 gacaactcca acatcctttc cgcgcgagtg gccaaggagc attaccgcgt tcccgccgtg
     2641 gtggcccgta tctacgaccc gcgccgcgcc cagatctatc aaaggttggg catccagacc
     2701 gtcgcaacgg tgaggtggac gaccgatcag atcctgcgaa ccctgatgcc ggacgaggtg
     2761 cccgtcgaat acaccgtcga caacggtgag gtcgtcgtga cggcgatcgc cgccccttcc
     2821 gagatcgtcg ggaagaaggc gaccgacgtc gacctggagg gccgcaggaa ggtcgttgct
     2881 gtcagcagat tcggggtccc gcgggttccg gagaaagacc tcgcgttgca ggatggagac
     2941 atccttcacg tttccgtcgt ccgtggcgat cttcctaccc tgaccgaggc cctcaagaag
     3001 gtggggggac cggagtgaga tcacgttcga tgcggatagc catcgccgga gccggcaacg
     3061 tcggaacctt cgtcgccaag gatctcgccg ccaagggcca c
//
LOCUS       DATMED010000208         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3609494, whole genome shotgun sequence.
ACCESSION   DATMED010000208 DATMED010000000
VERSION     DATMED010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609494"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..2003)
                     /gene="glmS"
                     /locus_tag="VNP73_05245"
     CDS             complement(168..2003)
                     /gene="glmS"
                     /locus_tag="VNP73_05245"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWL65362.1"
                     /translation="MCGIVGYVGSDEALPVLLDGMTKLEYRGYDSAGVAVLAEGTISV
                     TKKAGQIGALKEAVATDPPAGTLGIGHTRWATHGAPNDVNAHPHRDCSGDIAIVHNGI
                     IENHDVLRKELEDEGHTFTSETDTEVIAHLLESIVKERSCSLLEAVTAAVDRLEGAFA
                     LVCISAKEPDRIVVARQEPPMIVGAAEGVGLVASDIPALLAYTRDVIPLENGQIAEVT
                     PGRVRVFDFKGHEVEPKSVHVEWDIDAAEKAGFEDFMLKEIFEQPDAVRNTIRGRLTP
                     EGLVDLEEVTWDRDRIRSIDKIVIVACGTSFHAGLVARHAIEHWTRIPVELDRASEFR
                     YRDPVLDENSLVIGIAQSGETADTLAAVRFGKEMGATVVAITNVVGSSITREADAVVF
                     THAGPEIGVAATKTYLSQLVAINLFALYLAQERGTMPDDKMQATVDEMRRLPEMVEEV
                     LHISGDVQRAAKRYADAEDFLFIGRGVGHAVAMEGALKLKEISYIHAEGYAAGELKHG
                     AIALIEKNVPVVAILTGWRMYDKMLANVEEVRARGADTILVAPQGDERARSLANELFE
                     VPPTRPLLTPIIDTIPLQLLAYYIAKEKGLSPDKPRNLAKSVTVE"
     gene            complement(2301..>3229)
                     /locus_tag="VNP73_05250"
     CDS             complement(2301..>3229)
                     /locus_tag="VNP73_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWL65363.1"
                     /translation="PIAGDVGEVVDLDDGADRYVAHLIRTVPEALNGLRVVLDCAYGA
                     AWRLGPRAFKEAGADVIALHAEPDGSRINVDCGSTNLTELAVAVVKEGADLGLAFDGD
                     ADRVLAVDERGEEVDGDRIMGMMALHLHATGHLKNDRVVATVMTNLGFRRALEDNGIE
                     IVSAPVGDKFVAQAMVEHGAVLGGEQSGHIIFSEYSTTGDGILTGLQLAQILRTSADP
                     MSKLAHFYEPFPQVLINVRVASKDGLEAAEEIWAAVAAAEEGLGDDGRVLVRASGTEP
                     LVRVMVEATDEALAQRTATELSGVVTQALRTP"
BASE COUNT          563 a         1096 c          947 g          623 t
ORIGIN      
        1 cgtgcttgcc gtaacggcca ccggccaatc tgatggcggc ggacgcgacc gctctgccgg
       61 cacgttccat caggacgtgg gccggcgtgc cggcagcgat cgtggcctta tcgcgggcag
      121 ccatctcgcc cggtgtaagc agcggcttca tcgcttcttc gaggctccta ctcgaccgtg
      181 acgctcttag ccaggttacg cggtttgtcg ggtgatagcc ctttttcctt ggcgatgtaa
      241 taggcgagca gctgcagcgg gatcgtgtcg atgatcggcg tcagaagggg tcgggtcggc
      301 ggcacctcga acagttcgtt cgcgagcgag cgggcccgtt cgtcgccctg cggggcgacg
      361 aggatcgtgt cggccccgcg tgcacgtacc tcttcgacgt tcgcgagcat cttgtcgtac
      421 atccgccagc ccgtgaggat ggccacgacg gggacattct tctcgatgag agcgatcgca
      481 ccatgtttca gctcgcccgc tgcgtagccc tccgcgtgta tgtaggagat ctccttcagc
      541 ttcagagcgc cttccatcgc gaccgcgtgg ccgacgcccc tcccgatgaa gaggaagtcc
      601 tcggcatctg cgtagcgctt cgcggcccgc tgcacgtctc ccgagatgtg cagcacctct
      661 tcgaccatct ccggcaagcg acgcatctca tcgaccgtcg cctgcatctt gtcgtcgggc
      721 atcgtgccgc gctcctgagc caggtacagc gcgaacaggt tgatggctac gagttgcgac
      781 aggtaggtct tcgttgcggc taccccgatc tcgggaccgg catgcgtgaa caccaccgcg
      841 tcggcttcac gagtgatcga cgagccgacg acattcgtga tcgccacgac ggtcgcaccc
      901 atctccttcc cgaaacgaac cgctgcaagg gtgtctgccg tctcacccga ttgggcgatg
      961 ccgatgacga gtgagttctc atccagcacg ggatccctgt agcggaactc cgaggcccgg
     1021 tcgagttcca cggggatgcg ggtccagtgt tcgatcgcgt gtcgagcgac cagtcctgca
     1081 tggaatgacg tcccgcaagc aacgatgacg atcttgtcga tgctgcggat ccggtcgcgg
     1141 tcccacgtca cttcttccag atcgacgagc ccctctggcg tcaggcggcc gcggatcgtg
     1201 tttcgcaccg catcgggttg ttcgaatatc tccttcaaca tgaagtcttc gaagccggcc
     1261 ttctccgcgg catcgatatc ccattcgaca tgcaccgact tgggttcgac ctcgtggcct
     1321 ttgaagtcga agacccgaac ccgacccggg gtgacttccg cgatctgccc gttttcgaga
     1381 gggatgacgt cgcgcgtgta ggcgaggaga gcagggatgt ccgatgcaac aagtcccacg
     1441 ccttcagcgg cacccacgat catcggcggc tcttgccgag cgacgacgat gcggtcaggt
     1501 tccttcgccg aaatgcaaac cagagcgaaa gctccctcca agcgatcgac tgctgcggtg
     1561 accgcttcca gcagcgagca ggacctctcc ttcacgatcg actcgagcag atgagctatc
     1621 acttcggtgt cggtttcgga cgtgaaggtg tgcccttcgt cctcaagctc tttcctgaga
     1681 acgtcgtggt tctcgatgat cccgttgtga acgatggcga tatcgccact gcagtctcgg
     1741 tgtggatgcg cattgacgtc gttcggcgcg ccgtgcgttg cccaacgagt gtgcccgatc
     1801 cccagtgtcc cggccggcgg atccgttgca accgcctctt tgagcgcacc gatctggccg
     1861 gccttcttag tcacggagat ggtgccctcg gcgagcacgg ccactccggc cgagtcgtaa
     1921 ccgcggtact cgagcttggt catgccatcc aggagtaccg gcagggcttc atccgaaccc
     1981 acgtatccca cgatcccgca catacgacct cccctcacgc gcagccgagc gcgcggctag
     2041 acaaacctgt cgtcgagagg agccacctcg atgccggttt gtgacccggg atcgctcccg
     2101 gggtttgtcg acatcgtttc gaccgtggcc gaccgagggt cacccgccga gtgggcccac
     2161 attgattctt cgcaccggcc gaggccgccg cgtgaggccc tccgagatcc cctacctcgt
     2221 cgactcgcca cctcctaacg gcgagccctg gcgctagatc cggctactgc ttcctctcca
     2281 tccccaagga taaagcctgt ctagggggtc ctgagtgcct gagtgactac gccgcttaac
     2341 tccgtcgccg tccgctgcgc cagcgcctcg tcggtcgcct ccaccatcac ccgaacgaga
     2401 ggctcggtac ccgacgctcg caccagcacc cgaccgtcgt caccgagacc ctcctcggcg
     2461 gcggccaccg cggcccagat ctcctcagcc gcctccaacc catccttcga cgccactcgc
     2521 acgttgatca gcacctgcgg gaaaggctcg tagaagtggg caagcttcga catgggatcg
     2581 gcggaggtgc gcaggatctg cgccaactgc aacccggtca atatcccatc cccagtcgtc
     2641 gagtactcgg agaagatgat gtggccggac tgctccccgc cgagaacggc accatgctca
     2701 accatcgcct gggcgacgaa cttgtctcca accggcgcgc tgacgatctc gatgccgttg
     2761 tcttcgagtg ctcgtcggaa gcccagattc gtcatgaccg tagcgacgac acggtcgttc
     2821 ttgaggtgac cggtggcgtg aagatgcaat gccatcatcc ccatgatgcg gtcaccgtcg
     2881 acctcctcgc cccgctcgtc gacggcaagc acgcgatcgg catctccgtc gaatgcaaga
     2941 cctagatctg ctccttcctt cacgactgcc acagccagct ccgtcaggtt cgtcgaacca
     3001 caatcgacgt tgatacgaga tccatccggc tccgcgtgaa gcgcgatcac atccgccccg
     3061 gcctccttga atgcgcgcgg gccgagccgc catgcggcac cgtaggcaca gtcgaggacg
     3121 acacggagac cgttcagagc ttcgggcacc gttcggatca ggtgcgcgac gtaacgatcg
     3181 gcaccgtcat caagatcgac cacttcaccg acgtcaccgg cgatcggga
//
LOCUS       DATMED010000209         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2097645, whole genome shotgun sequence.
ACCESSION   DATMED010000209 DATMED010000000
VERSION     DATMED010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097645"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VNP73_05255"
     CDS             <1..1081
                     /locus_tag="VNP73_05255"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWL65364.1"
                     /translation="ILHLLNHSAGWSGDLMENTGDGDDAIAKYVARMADIEQVTPLGA
                     TASYNNAALSLAGLVIEKVTGKTYEQAMKELIFEPLGLSNMYFFPSEIMTRRFAIGHS
                     QEPDGTVKVTRPWAMPRGNSPAGGISSNSGDLIKWGLFHLGDGTARDGTRVLSEELLK
                     KMQQPTIEMPGSALGDAVGISWLLRDVDGVRLVSHGGDTIGQHSDFVMVPERNFVIST
                     LTNCGPNGNRFNDDIVKWALENYLGVIDKDPEALSLSSEELAPFTGRFETIAAWAEIK
                     PAEGHLLLEIEIKPETLKQLQEAGEDAPLEQPPFPLGLLPGEGDHYIVIDGPAKGMKG
                     YFVRGQGDAIDAVHVGGRLATRVKA"
     gene            complement(1078..1815)
                     /locus_tag="VNP73_05260"
     CDS             complement(1078..1815)
                     /locus_tag="VNP73_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65365.1"
                     /translation="MNEGLRGYALLFRWEFLRQRQQLPVIIVIQVVLAVGVVYGMSFL
                     LPNIDPQSALYLATGAPTLSLLIMGLVAVAQEVGRSKDTGREEYLRALPLPALAVLGA
                     TVTFWLLANLPGTALSLWVAKLRFGVQLDVTPLVIPVILLVALTASSVGYAMAQALTP
                     QVTQQLSSFLSIGILLFSPINFPIDRLPVALQQIHLVLPITYMADLIRWSLTGNSTHS
                     PGVGFAVLGAWCAAGLALSYRVATRRR"
     gene            complement(1812..2579)
                     /locus_tag="VNP73_05265"
     CDS             complement(1812..2579)
                     /locus_tag="VNP73_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65366.1"
                     /translation="MNSALLEVESVTKVYPRGIVANDDVSLHIDAGEIYGLLGPNGAG
                     KTTLVSQILGLIEPTSGSISIAGTDVTKKPKVARKACSYQPQTTASPEGLTPLQAVEI
                     VGRIRGLGAGSARARGKELMDALDIRRWADKRIPLSGGVGRLTSFCMAAVAPGRIVVL
                     DEPTNDVDPLRRKLLWEQVRLLAKRGSAVLLVTHNVLEAERSVDRLAIMVDGRLIAQG
                     TLDSLKSGLGALGSTSLEDVYARLVAPSMSVSEEAIV"
     gene            complement(2576..>2788)
                     /locus_tag="VNP73_05270"
     CDS             complement(2576..>2788)
                     /locus_tag="VNP73_05270"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65367.1"
                     /translation="ILSKAELEIAKRGGTKLSIFVIGVLRIQKSVTPALADETIEHIQ
                     IFGSIKGPQAVIDRLGERVMRGFGKE"
BASE COUNT          556 a          868 c          871 g          493 t
ORIGIN      
        1 gatccttcat ctcctcaacc actccgccgg atggtcgggc gacctcatgg agaacacggg
       61 cgatggcgac gacgcgatcg ccaagtacgt cgccaggatg gccgacatcg aacaggtcac
      121 gccgctgggt gccacggcct cgtacaacaa cgcggcgctg tcactcgccg ggcttgtgat
      181 cgagaaggtg acgggcaaga cctacgagca ggcgatgaag gagctcatct tcgaaccgct
      241 ggggctaagc aacatgtact tctttcccag cgagatcatg acccgccgct tcgccatcgg
      301 tcacagccag gaacccgacg ggaccgtaaa agtgacgcgg ccgtgggcga tgccgcgcgg
      361 caactctccg gcgggcggga tctcctccaa ctcgggggat ctcatcaagt ggggtctctt
      421 ccacctcgga gacggcaccg ctcgagacgg cacccgagtg ctgtcggagg aactgctcaa
      481 gaagatgcag cagcccacga tcgagatgcc gggcagtgct ctgggtgacg cggtcggcat
      541 ctcgtggttg ctcagggatg tcgatggggt gcggctggta tctcacggtg gtgacacgat
      601 cggtcagcac tccgacttcg tcatggtccc cgaacgcaac ttcgtcatct cgacgttgac
      661 gaactgtggg ccgaacggga accgcttcaa cgacgacatc gtcaagtggg ccctcgagaa
      721 ctatctcggc gttatcgaca aggaccccga agcgctatca ctgtcgtcgg aggagcttgc
      781 gcccttcacc ggtcgcttcg agacgatcgc ggcatgggcc gagatcaaac cggccgaagg
      841 tcatctcctg ctcgagatcg agatcaaacc cgagactctc aaacagctac aagaggcagg
      901 tgaggacgcc cctctggagc agccgccgtt cccgctcgga ctattgcccg gcgagggtga
      961 tcactacatc gtgatcgacg gtcccgcaaa gggcatgaag ggttacttcg ttcgtggcca
     1021 gggcgacgcg atcgacgccg tccacgtcgg aggtcgtctg gcgacgaggg ttaaggccta
     1081 gcgtcttcgg gtcgcgactc ggtaactgag ggccagtcct gccgcgcacc acgcgccgag
     1141 cactgcgaac cccacccccg gggaatgcgt gctgtttccg gtcaggctcc aacggatcag
     1201 gtccgccatg taggtgatgg gcaggacgag gtggatctgc tggagggcca ccggcagtcg
     1261 atcgatcggg aaattgatcg gtgagaacag caatatcccg atgctgagga acgaactgag
     1321 ctgttgggtt acctgcggcg tcaaggcttg agccatcgca tacccgaccg atgaggccgt
     1381 gagtgccacc aagaggatca ccgggatcac cagtggagtg acgtcgagct ggacgccgaa
     1441 ccggagtttc gccacccaca gcgatagtgc ggttccgggc aggttcgcaa gcagccagaa
     1501 cgtcacggtt gcacctagca ctgccagcgc gggcagggga agcgcccgga ggtactcctc
     1561 tcgaccggtg tccttcgacc gcccgacttc ctgcgccacg gccacgaggc ccatgatcaa
     1621 tagcgaaagc gtcggtgccc cggttgcgag gtatagagcg gactgcggat cgatgttggg
     1681 taacaggaac gacatcccgt aaacgacgcc gaccgccagc acgacctgga tgacgatgat
     1741 cacgggcagc tgctggcgtt gacgtaggaa ttcccaccgg aatagcaacg cgtagccgcg
     1801 caatccctcg ttcatacgat cgcctcctcc gacaccgaca tcgaaggggc gacgagtcgc
     1861 gcgtacacgt cttccagaga cgttgaaccc agcgcgccga ggccggactt gagtgagtca
     1921 agagtccctt gagcgatcaa acgtccgtcg accatgatgg ccaacctgtc caccgagcgt
     1981 tccgcttcca acacgttgtg ggttaccagc agaaccgccg acccccgctt ggctaagaga
     2041 cgaacctgct cccacaagag tttgcggcga aggggatcga catcgttcgt gggctcgtcc
     2101 aggacgacta tccggccggg cgcgaccgcg gccatacaga acgacgtcag gcgaccgaca
     2161 cccccggaca gagggatcct cttgtcggcc caacggcgga tgtcgagagc atccatcagt
     2221 tccttgcctc gggcccgcgc gcttccggca cccagtcccc ggatacgacc gacgatctcg
     2281 acggcctgga gcggcgttag cccttctgga gaggcagtcg tctgcggctg ataggagcac
     2341 gccttccgag caaccttcgg tttcttcgtg acgtcggtcc ccgcgatgct gatcgagccc
     2401 gacgttggtt cgatcaggcc gaggatctga ctaacgagtg tcgtcttgcc ggcaccgttc
     2461 ggaccgagaa ggccgtagat ctcaccggcg tcgatatgca aggagacgtc gtcgttcgcg
     2521 acgatgccgc gcgggtacac cttggtgacg ctctccactt cgaggagagc cgagttcatt
     2581 cttttccgaa cccgcgcatg acccgctcgc cgaggcgatc gatgacggct tggggtcctt
     2641 tgatcgaacc gaagatctgg atgtgttcga tggtctcgtc ggctagggcc ggcgttacag
     2701 acttctggat acggagcacc ccgatcacga agatgctgag cttcgtccct ccgcgcttcg
     2761 ctatctcgag ctcggctttc gacaagat
//
LOCUS       DATMED010000210         2460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_169633, whole genome shotgun sequence.
ACCESSION   DATMED010000210 DATMED010000000
VERSION     DATMED010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169633"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /gene="rimM"
                     /locus_tag="VNP73_05275"
     CDS             <1..317
                     /gene="rimM"
                     /locus_tag="VNP73_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWL65368.1"
                     /translation="KFSGVDSREDAEAMRGSLFVHKEQRRELDASEAWIDDLIGCEVR
                     TLDGVSVGRVTQVIPSPAQDLLSVATEAGERFVPMVKEIVVSIDVAAKTVTVDPPEGL
                     LG"
     gene            314..994
                     /gene="trmD"
                     /locus_tag="VNP73_05280"
     CDS             314..994
                     /gene="trmD"
                     /locus_tag="VNP73_05280"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWL65369.1"
                     /translation="MKIDVVTLFPEFFEAPLEASLLGKAIAAGVLEVRTVELRAHGLG
                     PHQSVDDEPYGGGAGMVMRPEPIFDAVESTRGEDGHVVLLSARGRRLVHSEVARLAGL
                     DHLVLICGRYEGVDERVALHLADEEISIGDYVLAGGEAAALVVIDAVSRLLPGVLGNA
                     ASLDTESHSSGLLEYPQYTRPADFRGWTVPEVLLSGDHGAVDSWRHEQAEAATRRVRP
                     DLMPLEDN"
     gene            999..>2460
                     /gene="uvrC"
                     /locus_tag="VNP73_05285"
     CDS             999..>2460
                     /gene="uvrC"
                     /locus_tag="VNP73_05285"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWL65370.1"
                     /translation="MARIGVQRPAAGTVPTRPGVYLFRDGHRRVVYVGKAKSLRSRIS
                     NYFGPELHPRTRAMLEAARDLEWIVTQNDVEALHLELNLIKQHRPRYNVRYRDDKSYP
                     YLAITLDEEFPRARVMRGSKNKGIRYYGPFAHAYAIRNTLDLLLRTFPMRTCSQGVFD
                     RCRRRNRPCLLYDIERCSGPCVGAVDADQHRAIALELCDFLDGNTKPVLTRLATAMKG
                     ASSREEYELAAKFRDQLDNVRKAIEKHQMVSSEREEVDVVGLVQDDLEAAFQVFYVRR
                     GRVTGRKGYIVDKVEDLATPELLAGFLERVYLDGDVPKQVLVPEEPAEAELIERWLRQ
                     LRGTKVAIRVPRRGEKKALLETVTENAQQAFTQHRMKRSSDFAARSRQLNELQEFLGM
                     DQAPLRIECFDISNTGPMEAVGSMVVFEDGLPKRSDYRRFAIKWTKGPDDVAMMGEVI
                     RRRFARYLDEKDSTPNKKARFAYPPNLVVIDGGKGQL"
BASE COUNT          451 a          747 c          814 g          448 t
ORIGIN      
        1 tgaagttctc cggcgtcgat tcgcgcgagg acgccgaggc catgagggga tcgctgttcg
       61 tgcacaagga gcaacgcaga gaactggatg cgagcgaagc gtggatcgac gatctcatcg
      121 gatgcgaggt ccgcaccctg gatggtgtca gtgtgggacg ggtgacccaa gtcatccctt
      181 cgccggctca ggacctgctg tcggttgcga ccgaagcggg tgagcgtttc gtcccgatgg
      241 tgaaagagat cgtcgtttcg atcgatgtcg cggccaagac cgtgaccgtc gatccaccgg
      301 aaggtctgct ggggtgaaga tcgacgttgt cactctgttt ccggagttct tcgaggctcc
      361 gttggaagca agcctcctcg ggaaggcgat agctgccggc gtgttggagg taagaaccgt
      421 cgagctccgc gctcacggac tcggccccca tcagagtgtg gatgacgaac cgtatggggg
      481 aggggccggc atggttatgc gcccggaacc gatcttcgat gctgtcgaat cgacccgcgg
      541 cgaggatggg catgtcgtcc tgctgtcggc gcgcgggagg cgtttggttc actcggaggt
      601 cgcccgcctc gcggggctcg accatctggt gctcatctgt ggtcgctatg aaggcgtgga
      661 cgagagggtc gccttgcacc ttgccgacga ggagatctcc atcggggact acgttcttgc
      721 cggtggcgag gcggcggccc tcgtcgtgat cgacgccgtc tcgcgtctcc ttccgggggt
      781 attggggaac gcggcgtccc tcgacaccga atcgcactcg tccgggctcc tcgaataccc
      841 gcagtacaca cgccccgccg actttcgcgg ctggactgtc cccgaagtgt tgctgtcggg
      901 tgaccacggg gcggtcgatt cttggcgaca cgagcaggcc gaggccgcca cccgcagggt
      961 gcgaccggac ctgatgccgc tggaagacaa ttaacgccat ggctcggatc ggcgtgcagc
     1021 gacccgcggc gggcaccgtt cccacgagac ccggcgttta tctcttccgg gacgggcacc
     1081 ggcgcgtcgt gtacgtcggg aaagcgaaat ctctccgcag tcgcatctcg aactatttcg
     1141 gccctgaatt gcatccgcgg accagggcga tgctggaagc agctagggat ctcgagtgga
     1201 tcgttaccca gaacgacgta gaggcgctcc atctcgagct caatctcatc aagcagcacc
     1261 ggccccggta caacgtccgc tatcgagacg acaagagcta tccctatctg gcgataacgc
     1321 tcgacgaaga attcccccgg gcccgggtga tgcgcgggag caagaacaag gggatccgct
     1381 actacgggcc tttcgcccat gcctatgcga tccgcaacac gctggacctc ttgctgcgga
     1441 cattcccgat gcgtacttgc tctcagggtg tgttcgaccg gtgccggcgc cggaaccgtc
     1501 cctgcctcct atacgacatc gagcgttgct cgggtccgtg cgtcggagcc gtcgacgcgg
     1561 accagcaccg ggcgatcgcg ctcgagctgt gcgatttcct cgacggcaac accaagccgg
     1621 ttctgacccg cctcgccacg gcgatgaagg gcgcatcgtc gcgtgaggag tacgaattgg
     1681 ccgcgaagtt ccgcgatcag ctcgacaacg ttcggaaagc gatcgagaaa catcagatgg
     1741 tctcgtccga gagagaggag gtcgatgtcg tcggcctcgt ccaggacgac ctcgaagctg
     1801 ccttccaggt gttctacgtc cgacgcggcc gggtcacggg ccgcaagggt tacatcgtcg
     1861 acaaggtcga ggacctggct acgcccgagc ttctggccgg cttcctcgaa cgtgtctatc
     1921 tggacggcga cgtgccgaag caggttctgg ttcccgagga gccggcggag gcggagctga
     1981 tcgaacgctg gttgcgccaa ctgcggggta cgaaggtggc gatccgcgtc ccgcgtcgcg
     2041 gcgagaagaa ggccttgctc gagaccgtta cagagaatgc acagcaggcg ttcactcagc
     2101 accggatgaa gcgctcgagt gacttcgccg cgcgctctcg tcagttgaac gaactgcaag
     2161 agttcctggg gatggaccag gccccgttgc gcatcgagtg tttcgacatc tcgaacaccg
     2221 gaccgatgga ggcggtcggc tcgatggtcg tattcgagga tggcctcccg aagcgatccg
     2281 actaccgcag attcgcgatc aagtggacaa aaggtcccga cgacgtggcg atgatgggcg
     2341 aggtcatccg gcggcgcttc gcccgctacc tcgacgagaa ggactccacc ccgaacaaga
     2401 aagcgcgctt cgcctatccg cccaacctcg tcgtgatcga cggaggtaag gggcagctga
//
LOCUS       DATMED010000211         7882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_541002, whole genome shotgun sequence.
ACCESSION   DATMED010000211 DATMED010000000
VERSION     DATMED010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541002"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VNP73_05290"
     CDS             complement(<1..195)
                     /locus_tag="VNP73_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWL65371.1"
                     /translation="MDLALGLFAVFVLILIHAFFVAGEFGLVTVDRTRVERMAEEGAR
                     GAAGVLAALRTLSFQLSGAQL"
     gene            270..1604
                     /locus_tag="VNP73_05295"
     CDS             270..1604
                     /locus_tag="VNP73_05295"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWL65372.1"
                     /translation="MAGASLDTIVNLCKRRGIVFQSSEIYGGLRSAWDWGPMGVELKR
                     NIKAEWWRAMVHMRDNVVGLESSILMSPKVWEASGHVETFTDPLVECTNCHHRFRADH
                     IDLAKACPDCGTKDAWTEPKLFHLMFQTHMGPVQDSAHTVYLRPETAQGMFVDFKTVQ
                     ATSRKRIPFGIAQQGKSFRNEITPGNFVFRTREFEQMEMEFFVKPGTDDEWHDYWLNE
                     RMNWYLDLGMTSDNLRLREHEADELSHYAKRTYDIEYDFPGMGWSELEGIANRTDFDL
                     KAHSESSGEDLSYFDPETEERYVPYVIEPAVGVERPLMAFLVDAYTEDEAMSGQGKME
                     KRTVLKIDKRLAPIKVAVLPLSKHADLVPVAEKVAAEIRPRLFTEVDVTQAIGKRYRR
                     QDEIGTPYCVTIDFDTLEDQAVTIRDRDSMQQDRVSLDKLVTYLSERFEGDE"
     gene            1604..1855
                     /locus_tag="VNP73_05300"
     CDS             1604..1855
                     /locus_tag="VNP73_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWL65373.1"
                     /translation="MPTLTGKVTVEGSPTKTATVELHNSQGDVIDQVQTNDDGQYTYH
                     LSAGSWTLNVWDERGNRAKQEVTLSEDDTTVDLELAPGK"
     gene            1916..4762
                     /gene="ppdK"
                     /locus_tag="VNP73_05305"
     CDS             1916..4762
                     /gene="ppdK"
                     /locus_tag="VNP73_05305"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWL65374.1"
                     /translation="MTKWVYDFHEGDASQKNLLGGKGANLAEMTNLGLPVPPGFTLTT
                     EACNAYREGGQQFPNGLLDEVADHRKHLEENMGREIGNARDPLLVSVRSGAPFSMPGM
                     MDTVLNVGLNDESVEGLVAQTQSDRFAWDSYRRLVQMFGKTVMGVEGDLFEDAIDDAK
                     ERKGVKNDVDLGTDDLKALVERFKEIVQKETDRGFPQDPTEQLHLAIEAVFKSWDNKR
                     AQDYRRRNRIPDSLGTAVNVQTMVFGNKGNDSGTGVAFTRDPSSGEKRPYGDYLVNAQ
                     GEDVVAGIRNTIPLEDMGAEQPDAFVELKQHMDTLEKHFKDMCDIEFTVEERKLWLLQ
                     TRVGKRTALAEWVIAFDMVEEGLIDEATAVRDRLTAGKLDELFKPVLKPESEPVAKGL
                     NASPGGAVGKAVFTADDAHEWAERGEKVILVRRETTPDDYHGMIASQGILTSAGGTNS
                     HAAVVARGEGIPAVCGADAIRIDRKAKKFTVGKETVAEGDWVSIDGTSGNVYVEESDV
                     EASELESAINGDSEARTSKMWSAYQRFMGIADQARRMRVRANADTPDQSRNARERGGE
                     GIGLCRTEHMFLGEERVAAVRKMIFADTEEDAKKAFDFLLPLQKEDFLGIFEAMDGLP
                     VTVRLLDPPLHEFLPDQVDLAIEVALAEDAWKRQEARGSVESITDRVAELVTSAADKV
                     PMGDLIKQLLETIGIDGQNEDEHLIPREDLEKKKTLLQKVNELHEANPMLGLRGVRLG
                     ILKPGLYAMQVRAIAEAAIELKKKGKDPRPEIMIPLVATAEELRQMREELEPMVKKMI
                     GEAGIELHVEFGTMIELPRACVEAKAIAQYADFFSFGTNDLTQTAFGFSRDDIGKFLA
                     MYEERKLVKTNPFVTIDEEGVGRLMQLAAWEGKESNGTLHLGICGEHGGDPESVVFCE
                     KTGLDYVSCSPFRVPVARLAAAQAVMGEATTSSK"
     gene            complement(4731..5603)
                     /locus_tag="VNP73_05310"
     CDS             complement(4731..5603)
                     /locus_tag="VNP73_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65375.1"
                     /translation="MGRWIKAGIVVTAGFAVLILLAAFVWSRLATFGYVKPPTDPRVG
                     GDIAAAKSDRAGFRVLFVGNRFTENNDMPDLVRYLADVDGPRRSLITVSYTGPEWSLR
                     YFEQEGRLIDLIESVDWDIVVLQEQSQLPGKYTRGEKMYPPARDLHHTIENSGANTML
                     FMTWGHREGDPFIADDSFELMTNRLREGYEILGNELGAEVAPVGLAWAEAMRRDPTID
                     LWTAGGKYPSVIGSYLTACVFHQVLSEEDPGTSPFTGDLSPHQARFLQDVARDVVAGY
                     SLSRVTSSSSLPLP"
     gene            complement(5630..6265)
                     /locus_tag="VNP73_05315"
     CDS             complement(5630..6265)
                     /locus_tag="VNP73_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65376.1"
                     /translation="MRWVSSFVVVMLLVAAVPGAAAIQQMTAAKPPTVEGPLTPQEPR
                     CDRKRQIYKGEVAGVAKRCLHFYAYSPASESDFGNDYGVLWLQSTVDGKNGWCASRVN
                     PKILLPEGLGLHARAPTEGKKVAGRSAMTTDLALDANGNGEPARVKQDWIAYPESFST
                     TTAEDNTLFKLSWTGKTGKPLGFAHGIEVSWPTDAPPEGISVRLDFRLAAC"
     gene            6310..7275
                     /locus_tag="VNP73_05320"
     CDS             6310..7275
                     /locus_tag="VNP73_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWL65377.1"
                     /translation="MSIRERLEAQEQAVLADRAQPAASSKGRDRLEEEDSLRTCFQRD
                     RDRIVHSKAFRRLAHKTQVFLAPEGDHYRVRLTHTLEVTHIARAIARARALNEDLTEA
                     ICLGHDLGHTPFGHLGEEVVAAFHPGFKHNLQSVRIVEVLEDLNLTWEVRDGIANHTW
                     TMPPPATLEGQIARFADRIAYINHDIDDSLRAGLLAPEDLPPAAKALGASPGDRIETM
                     VGDLVEASHDADEIQMSDSIFESMSEMRSFLFERVYMGVVAPATRHSVELIVSSLLEH
                     FSAEPGEAGRIEAVDYVAGMTDRFALRAFEQVVGRPPPGLEAQLG"
     gene            7323..>7882
                     /locus_tag="VNP73_05325"
     CDS             7323..>7882
                     /locus_tag="VNP73_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HWL65378.1"
                     /translation="MAKIKEEDIDALREKADIVELVSGYTPLKKSGANTFKGLCPFHS
                     EKTPSFSVNSGTGLFHCLSGETGVITWAGTFPIEKLAGETHRLLSKRPGGQPFWVDAE
                     IKSFGVQQLYEIKLSRNRRKKSIFATDGHRWFVRGGKRQPLKEKVTAKLVAGDSLPSV
                     FARARAGLPVPPSPLGIAHGFTFGDG"
BASE COUNT         1614 a         2382 c         2514 g         1372 t
ORIGIN      
        1 gagttgggca cccgagagct ggaaggagag cgtccttaac gccgcaagta ctcctgccgc
       61 gccgcgggcc ccctcctcgg ccatgcgctc gacacgcgtc cgatcgactg tgacgagacc
      121 gaactcgccc gcgacgaaga aagcgtggat gagtatgagt acgaagacgg cgaacagccc
      181 taaggcgagg tctataaggg cccgctctcc ggggtcggtg ctgccatgta ggcctctatc
      241 atgccctcag acccgaaagg agtggcgttt tggccggtgc ttcgctcgac acgatcgtca
      301 acctgtgcaa gcgcaggggc attgtgttcc agtcctcaga gatatacggg ggcctgcgct
      361 cggcgtggga ctggggccca atgggggtcg agctcaagcg gaacatcaag gccgagtggt
      421 ggcgggccat ggtccacatg cgtgacaacg tggtgggcct ggagtcttcg atcctgatgt
      481 ctcccaaggt atgggaggcg tcggggcacg tcgagacgtt caccgaccct ctcgtggagt
      541 gcacgaattg ccaccatcgc ttccgggcag atcacatcga tctcgccaag gcctgccccg
      601 attgcggcac caaggacgcc tggaccgagc cgaagctctt ccatctgatg ttccaaaccc
      661 atatggggcc ggtgcaggat tcggctcaca ccgtctacct gcggcccgag accgctcagg
      721 ggatgttcgt cgacttcaag actgtgcaag cgacgtcacg caagaggatc cccttcggca
      781 tcgcgcaaca ggggaagtct tttcgcaacg agatcacccc gggcaacttc gttttcagga
      841 cgcgtgagtt cgagcagatg gagatggagt tcttcgttaa gcccggcacc gacgacgagt
      901 ggcacgacta ctggttgaac gagcggatga attggtacct cgacctcggg atgacgagcg
      961 acaacttgcg cttgcgcgag cacgaggccg acgagctcag tcactatgcg aagcgcacat
     1021 acgacatcga gtacgacttc cctgggatgg ggtggtcgga actcgaaggg atcgcgaacc
     1081 gcaccgactt cgatctgaag gcgcactccg agagctcggg cgaggacctc tcctacttcg
     1141 atcccgagac ggaagaacgt tacgtgccgt acgtgatcga gccggcggtc ggtgtcgagc
     1201 gcccgctgat ggccttcctg gtcgatgcat atacggagga cgaggccatg agcgggcagg
     1261 ggaagatgga gaagcgcaca gtgctgaaga tcgacaagcg tctcgccccg atcaaggtcg
     1321 cggtgctgcc tctctcgaag cacgccgacc tcgtgcccgt cgccgagaag gtcgcggccg
     1381 agatcaggcc ccgactattc accgaggtcg atgtcacgca ggcgatcggc aagcgctatc
     1441 gcaggcagga cgagataggt acgccctact gcgtgaccat cgacttcgat accttggagg
     1501 accaagcagt gacgatcagg gatcgggact cgatgcagca ggatcgggtt tcgctcgaca
     1561 agctggtgac atatctatcg gaacgatttg agggggacga gtgatgccaa cgcttaccgg
     1621 gaaagtaacc gtagaaggca gcccgaccaa gacggcaacc gtcgagctgc acaactcgca
     1681 gggtgacgtg atcgaccagg tccagacgaa cgatgacggg cagtacacct atcacctgtc
     1741 ggccggaagc tggacgctga acgtgtggga cgagcgcggg aaccgggcga agcaggaggt
     1801 aacgctcagc gaggacgaca cgaccgtcga tctcgaactg gcaccgggca agtgatcaag
     1861 acggcgcccc tcgccgctcc gcggcggcgc cttacttgag gtagaaggga accagatgac
     1921 taagtgggtc tacgacttcc acgaagggga cgcgtctcag aagaatctcc tcgggggtaa
     1981 gggcgcgaac ctcgccgaga tgacgaacct ggggcttccc gtgccccccg gtttcacgct
     2041 caccaccgag gcctgcaacg cgtacaggga aggcggacag caattcccta acggcctcct
     2101 cgacgaggtc gccgaccacc gcaagcatct ggaagagaac atgggccgtg agatcgggaa
     2161 cgcgcgcgat ccattgctcg tttccgtgcg ttcgggcgcg ccgttctcga tgccggggat
     2221 gatggacacg gttctcaacg tgggcctcaa cgacgagagc gtcgaggggc tcgtggccca
     2281 gacccagtcg gatcgcttcg catgggactc gtatcgccgt ctcgtgcaga tgttcggcaa
     2341 gacggtgatg ggcgtcgagg gcgacctgtt cgaagacgcg atcgacgacg ccaaagagag
     2401 gaagggcgtc aagaacgacg tcgatctcgg caccgacgac ctgaaggcct tggtcgaacg
     2461 tttcaaggag atagttcaga aggagaccga tcgaggcttc ccacaggatc ctacggagca
     2521 gctgcacctc gcgatcgaag ccgtgttcaa gtcgtgggac aacaagcgcg ctcaggacta
     2581 ccggcggcgt aaccgcatcc ccgattcgct gggtaccgca gtgaacgtgc agaccatggt
     2641 gttcggcaac aagggcaacg attccggtac gggcgtcgcg ttcacgcgcg acccttcgtc
     2701 gggtgagaag cggccctacg gcgactacct ggtgaacgct cagggtgagg acgtcgtggc
     2761 cgggatccgg aacacgatcc ccctcgagga catgggggcc gagcaacccg atgccttcgt
     2821 cgagctgaaa caacacatgg acacactcga gaagcacttc aaagacatgt gcgacatcga
     2881 gttcaccgtc gaagagcgca aattgtggct gctacagacg cgcgtcggga agcgaactgc
     2941 gttggccgag tgggtcatcg cgttcgacat ggtcgaggag ggcctgatag acgaggccac
     3001 ggcggttagg gatcgcctta ctgcaggcaa gctcgacgag ttgttcaagc cggtcctcaa
     3061 gcccgagagc gagcccgtcg ccaagggact gaacgcatct ccgggtggtg cggtcggcaa
     3121 ggccgtgttc accgccgacg acgcccacga gtgggcggag cgcggagaaa aagtcatctt
     3181 ggttcgtcgc gagacgactc ccgacgacta ccacgggatg atcgcctcgc aagggatcct
     3241 cacatctgcc gggggcacca actcgcacgc ggccgtcgtg gcccgaggcg aaggcatacc
     3301 tgcggtttgc ggtgccgatg cgatcaggat cgatcgcaag gccaagaagt tcacggtcgg
     3361 caaagagacc gtcgccgaag gcgactgggt ctcgatcgac ggcacttccg gcaacgttta
     3421 cgtcgaagag tcggacgttg aagcgtccga gctcgagtcg gccatcaatg gggactccga
     3481 agcccgcaca tcgaagatgt ggagtgcgta ccaacgcttc atggggatcg ccgatcaggc
     3541 acgcaggatg cgcgtgcggg ccaatgccga cacgccggac cagtctcgca atgctcggga
     3601 acgcggcgga gaaggcatcg gtctgtgccg gaccgagcac atgttcctcg gcgaggagcg
     3661 tgtcgctgcc gttcgcaaaa tgatcttcgc cgacaccgaa gaggacgcga agaaggcgtt
     3721 cgatttcctc ctgccgctgc agaaggagga cttcctcggg atcttcgagg cgatggacgg
     3781 gctgcccgtc accgttcgac ttctcgaccc cccgctgcac gagttccttc ccgatcaggt
     3841 cgacctcgcg atcgaggttg ctctcgcgga ggacgcatgg aagagacaag aagcgagagg
     3901 cagcgtcgag tcgatcaccg atcgggtggc cgaactcgtg acctctgccg ccgacaaagt
     3961 gccgatgggc gatctcatca agcagctact cgagacgatc gggatcgacg ggcagaacga
     4021 ggacgagcat ctcatcccgc gtgaagacct cgaaaagaag aagacactcc tacagaaggt
     4081 caacgagctt cacgaggcga acccgatgct ggggttgcgc ggcgtccgtc tggggatcct
     4141 gaagcccggc ctttatgcaa tgcaggtgcg tgcgatcgcc gaggccgcga tcgagctcaa
     4201 gaagaagggc aaggatccgc gacccgagat catgatcccg ctcgtcgcca cggctgaaga
     4261 gctgcgtcag atgcgcgagg agctcgagcc gatggttaag aagatgatcg gcgaggccgg
     4321 catcgagttg cacgtcgagt tcgggacgat gatcgaacta ccgagggcat gcgtcgaagc
     4381 caaagcgatc gcccagtacg cagacttctt ctcgttcggt acgaatgacc taacgcaaac
     4441 ggcgttcgga ttctcgcgcg acgacatcgg caagttcctg gcgatgtacg aggagcgcaa
     4501 gctcgtcaag acgaacccat tcgtaacgat cgacgaggaa ggggtcggac ggcttatgca
     4561 gctcgccgcg tgggagggca aggagtcgaa cggcaccctt catctcggca tctgcggaga
     4621 gcatggggga gaccccgagt cggtcgtgtt ctgcgagaag accggcctcg attacgtctc
     4681 gtgctcgcca ttccgtgtcc cggtagcgcg gctcgccgcg gcgcaggccg ttatgggaga
     4741 ggcaacgacg agctcgaagt aactcgtgag agcgagtacc cggccacgac gtctctcgcc
     4801 acatcttgta gaaatctcgc ttgatgaggc gataggtcgc cggtgaacgg gctcgtaccc
     4861 ggatcttctt cagacagcac ctggtggaat acgcatgcgg tgagatagga accgatcacg
     4921 ctcggatatt tgccaccggc cgtccagaga tcgatcgtcg gatcccggcg catggcttcg
     4981 gcccaggcga gacccaccgg tgcgacttcg gcacccaatt cattgcctag gatctcgtac
     5041 ccctcccgga gccggttcgt catcaattca aatgagtcgt ctgcgatgaa cgggtctccc
     5101 tcccggtgcc cccaagtcat aaacagcatg gtgttcgccc cgctgttctc tatcgtgtgg
     5161 tggaggtcgc gagcgggcgg gtacatcttc tccccccttg tgtacttgcc cggaagctgg
     5221 ctctgttcct ggagcacgac gatgtcccag tcgacactct cgatcaggtc gatcagtctc
     5281 ccttcctgtt cgaagtacct caggctccac tccgggccgg tataggacac ggtgatgagc
     5341 gaacgacgtg gcccgtctac gtccgcgaga taacgcacga ggtcgggcat gtcgttgttc
     5401 tcggtgaagc gattccccac gaacaagacg cggaatccgg cccgatcgct cttcgctgct
     5461 gcgatgtcgc caccgacgcg tggatcggtc ggcggtttga cgtaaccgaa ggtcgccagc
     5521 ctgctccaga cgaaggcggc gaggagtatg agcacggcga aacccgccgt gacgacgatc
     5581 cccgccttga tccaccgccc cataggggca gattacaaat gtgcgccggc tagcaggcgg
     5641 ccaagcggaa gtccagcctg acggagatgc cttcgggtgg agcgtccgtc ggccacgaca
     5701 cctcgatacc gtgcgcgaag cccagcggct ttcccgtttt gccggtccaa ctgagcttga
     5761 agagggtgtt gtcttccgct gtagtcgtcg agaacgactc cgggtatgcg atccaatcct
     5821 gtttcacccg agccggttca ccgttgccat tggcatccag agccaggtcc gtcgtcatcg
     5881 cagagcgccc ggctaccttc tttccttcgg tcggggccct cgcgtgcaat cccagaccct
     5941 cggggagcag gatcttcgga ttgacccggg acgcgcacca tccgttcttc ccgtcgaccg
     6001 ttgactggag ccacagcact ccgtagtcgt tcccgaagtc ggattccgac gcaggggagt
     6061 aggcgtagaa gtgcagacac cgcttggcga ccccggcgac ctctcccttg tagatctgtc
     6121 tcttccggtc gcatctcggt tcttgcgggg tgagcgggcc ttcgacggta ggaggtttcg
     6181 cggccgtcat ctgctggatc gccgcggccc ccgggacggc ggccacaagg agcatcacga
     6241 cgacgaagct agacacccac cgcatacgta gcctcacgat acgtcacacc ccagtgctac
     6301 tttcccgata tgtccatcag ggagcgcctc gaagcgcagg agcaggcggt gttggccgac
     6361 cgggctcagc cggccgcatc ctccaagggc cgcgaccgcc tcgaggaaga ggattcgctc
     6421 aggacctgct tccagcggga ccgggatcgg atcgtccaca gcaaggcatt ccgcaggctg
     6481 gctcacaaga cccaggtgtt cctcgctcct gaaggcgatc actaccgcgt tcggctcacc
     6541 catacgctcg aggtcaccca catcgccaga gcgatcgccc gtgcccgcgc gctgaacgag
     6601 gacctcaccg aagcgatctg cctcggccac gacctgggcc acacgccgtt cggccacctc
     6661 ggcgaggagg tggtggccgc gttccacccg ggcttcaagc acaacctgca gtcggtgcgg
     6721 atcgtggagg tgttggaaga cctcaacctg acatgggagg tccgagacgg gatcgcgaac
     6781 cacacctgga cgatgccccc gcccgccacc ctcgagggtc agatcgcacg cttcgcagat
     6841 cgcatcgcct atatcaacca cgacatcgac gactcgctgc gggccgggct gctggctccc
     6901 gaggatctgc cgcctgcggc caaggctctg ggggcgagcc ccggcgaccg gatcgagacg
     6961 atggtgggcg atctcgtcga ggccagtcac gacgcggatg agatccagat gagcgactcg
     7021 atattcgagt ccatgtccga gatgcgctcg ttcctgttcg aaagggtcta catgggcgtg
     7081 gtcgccccgg ccacccggca ttcggtcgag ctgatcgtct ccagtctgct cgagcacttc
     7141 tcggccgagc cgggcgaagc cgggcggatc gaggccgtcg actacgttgc ggggatgacc
     7201 gaccgtttcg cgctcagggc tttcgagcag gttgtcggcc gtcctcctcc gggcctggag
     7261 gcgcagctag gatgaagaga cttccccgtc taggccactt aaacgcgctt ggaagaagct
     7321 agatggcgaa gatcaaagaa gaagacatag acgccctccg ggaaaaagcc gacatcgtcg
     7381 agctggtctc cggttacacg ccgctgaaga agtccggtgc caacacgttc aaaggtctat
     7441 gtcctttcca cagcgagaag accccgtcgt tctccgtcaa ctcaggtacg gggttgttcc
     7501 attgtctttc cggagaaacg ggtgtaatca cttgggccgg aacattccca atcgagaagt
     7561 tggccggtga gacacaccga ctgctgtcta agagaccggg aggccagccg ttttgggtgg
     7621 acgcggagat caagtcgttc ggagttcagc agctctacga gatcaagctc agtaggaacc
     7681 gccgcaagaa aagcatcttc gcgacagatg gtcaccgctg gtttgtccgt ggagggaagc
     7741 gccaaccgct aaaagagaaa gtcaccgcca agctggttgc gggggattcc ctgccatccg
     7801 tttttgcacg cgcaagagcg ggtttgcccg tccctccctc gccgttgggg atcgcacatg
     7861 ggttcacatt cggggatggt ca
//
LOCUS       DATMED010000212         6069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4003294, whole genome shotgun sequence.
ACCESSION   DATMED010000212 DATMED010000000
VERSION     DATMED010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003294"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VNP73_05330"
     CDS             complement(<1..304)
                     /locus_tag="VNP73_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65379.1"
                     /translation="MDIFFTILNLTAFYFISETFGSVRSGVLDGAPNYFAFASVGMAM
                     TLVIQTASVGMGTRVREEQLTGTLEYLITQPVTSTEIVLGLTGFTFAFATVRAAFYI"
     gene            complement(384..1013)
                     /locus_tag="VNP73_05335"
     CDS             complement(384..1013)
                     /locus_tag="VNP73_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65380.1"
                     /translation="MNSSADVLQISDLSKRFGDKQVLSSLDLAVSPGERVAIVGPNGS
                     GKTTLLRCIAGTLLPDHGSLTVAGFPPGSLEAQRRTGVSLSQERSFYLRLTGLQNLRF
                     FARIKGISADAAPREVGSLIEELELKEIVVPRMARCSTGMLQQVSLARSLLGSPQLIL
                     LDEPTRSLDQDARERLWKALERRTGSGIMVATHLPSDIERCTRVIDLAA"
     gene            complement(1020..1667)
                     /locus_tag="VNP73_05340"
     CDS             complement(1020..1667)
                     /locus_tag="VNP73_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65381.1"
                     /translation="MADKDSFQIVFLCTGNRARSAMAEAFVRKMTQDLPVEVSSAGLL
                     ELEAGPALPEAVLASSRVGIDLSEHRSRPITQTKFDHTDLVLGFERQHISTAVVDGGA
                     DPGKSFLLRELLRLLDSLPLEDPSGDGIERAKMKVASAHRFRVTAPNFSVDDEIADPY
                     GKEERVFDRLANELYRSCAALTESLFGVKVSLIDIPEPVGSTRPKGGFLGKLKRG"
     gene            complement(2065..3714)
                     /locus_tag="VNP73_05345"
     CDS             complement(2065..3714)
                     /locus_tag="VNP73_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL65382.1"
                     /translation="MRRVAGVIAMVSIVLASLVSPAPAQESPTMIPMSDGAHLKAEIL
                     LPTEGKRFPVLLNMTPYGPATYFSTYLGNGYAHVNVDIRGTGGSEGALCIFCDREQQD
                     VYDVVEWVARQKWSNGKVGMYGGSYQGITPILGASKQPPHLEAIIPAVTYADAYRDIV
                     WHNGIFNANFVAQWLTAQTALSMTGTAPSSDLAARVGQRMVFESRLQPWDDAFYKERS
                     VNTKYDRIKVPILHMGAWFDGFSRGTLDNFAGVASKHNRLIMVPCTHKGCGGPFDPAS
                     EYTADAEAPGPDDIYLAWFEHFLKGKDNGIEDGPPVLFFDIGSNEWVTDTAWPPRDSK
                     LKTFYLSGAASGSSSEVVDGSLVEKPVDDQDVEDHYVYDPTVGTSETFAKWGTVALSP
                     HYRADQRPDDARSLGYTTDAVKKPLRLAGPMELHFWGATTASDTDWIVKVTDVAPDGG
                     SKLITSGYVRASHRKWDPKRSRPGRPWLSNTSAAQVPSGKTLEYRIDIWDIAHTVLEG
                     HRLRISLSSSDTPNHEPLPEPALNYLFHNGSYPSRLLVTVR"
     gene            3816..4373
                     /locus_tag="VNP73_05350"
     CDS             3816..4373
                     /locus_tag="VNP73_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWL65383.1"
                     /translation="MTPRIPPTTAAERDERTEELLSNMFRRPDGTDLNIFTTLAHHPK
                     LLKRWAQFGGTLLFGGTLSGRDREMLILRTAWLCDAEYEWGQHVTIGLNSGLTEDEIE
                     RIHKEGAAGEWAENERALLQAADELHADHRISDETWKVLDSAYDYQQLIEICMVVGQY
                     HMVAFTLNSLGIEPEPGLPEFPNKG"
     gene            complement(4375..5181)
                     /locus_tag="VNP73_05355"
     CDS             complement(4375..5181)
                     /locus_tag="VNP73_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL65384.1"
                     /translation="MTGERLAGKTAIVVGAGQTPGATIGNGRATALRFAQEGAHLLLV
                     DRDDASVKETQALILEDGGKADVAVVDISLPGGPAEIAEQGKAILGRIDILHNNVGIG
                     MGDNTPTKLAEEAWDRIFDVNLKAMWLTCKEVVPIMRRQNGGAIVCVSSLAAIAAAGH
                     ITAYKISKAGVNALVHSLAITNAHHGIRVNGIMPGFIDTPMAVDAVASALDIDRDDLA
                     KLRAEMVPLKKQQGTAWDVANAALFLASNEAAFITGAVLPVDGGQSSKIG"
     gene            complement(5215..>6069)
                     /locus_tag="VNP73_05360"
     CDS             complement(5215..>6069)
                     /locus_tag="VNP73_05360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWL65385.1"
                     /translation="IEANGIHVLGHIDELDHVIFTNHADCLFVASSAVGPTDMLRVAK
                     AGRRLGVDVRVSANVPEMLTSRLTIQPVGDTMSLTLRPVRLSGAQALTKRIFDLVLSS
                     VVLVFITPVLAAIAAAIKLTSPGPVFFRQHRVTKNGHVFPVFKFRTMRTDGDEILKQR
                     GIDPSVPFFKLEDDPRITRVGRFIRRTSLDELPQLLNVIRGEMSLVGPRPLPVDQVMA
                     NENLLSPRHEVSSGITGWWQVNGRSDVSPKKALNLDIFYIENWSLSLDLYILLKTAQV
                     LLKRQGAY"
BASE COUNT         1123 a         1887 c         1857 g         1202 t
ORIGIN      
        1 ggatgtaaaa cgcggctcgc actgtcgcga acgcgaacgt gaatcccgtc aacccaagaa
       61 cgatctctgt agacgtaacg ggttgcgtta tcaggtactc gagagttccg gtcagttgct
      121 cttcgcgcac gcgcgtgccc atccccacac tcgccgtctg gataacaagc gtcatcgcca
      181 tccctaccga cgcgaaggcg aagtaatttg gtgcgccgtc caatacccct gatcgcacag
      241 agccgaatgt ctcggagatg aaatagaagg ccgtgagatt caggatcgta aagaagatgt
      301 ccatcacaaa ggcagttcga tacgacctcg tgatcagcca gtcccggcct gtcatcgcga
      361 gaacgatcga gagtcgagga aacctaggca gcaagatcga tgaccctcgt gcatcgttcg
      421 atgtcgctcg gcaagtgcgt agctaccatg atccccgaac ccgttcgcct ctccaacgct
      481 ttccacagtc gctcacgtgc gtcttgatcg agggagcgcg tcggttcgtc cagcagtatt
      541 agctgtggtg atcccagaag ggatcttgcc aaactgactt gctgcagcat tcccgttgaa
      601 cagcgggcca tccttggcac cacgatctcc ttcagttcca gttcttcaat cagcgatccg
      661 acttcacgcg gggccgcgtc ggccgatatg cccttgatgc gggcgaagaa gcgaaggttt
      721 tgaagtccgg ttaaacgcag atagaaggac cgctcctggg aaagagagac tccggtacgt
      781 ctctgtgctt ccaacgagcc cggcgggaac ccggcgaccg tgagcgaacc gtggtcaggc
      841 aggagggttc cggcgataca ccggaggagt gttgtcttac cgctcccatt tggtccgacg
      901 atggcgaccc gttcgccggg cgaaaccgcc aggtctagtg aagacaaaac ctgcttatcg
      961 ccgaatctct ttgacagatc gctgatctgt aggacgtcgg cggaactatt cactacctat
     1021 cagccgcgct ttagttttcc gaggaaaccg cccttaggtc gggtcgagcc caccggctcg
     1081 gggatgtcga taagcgatac ctttactccg aacagagact cagtcagcgc cgcgcacgag
     1141 cggtagagct cgttggccag ccggtcgaac acccgttctt ccttcccgta gggatcggcg
     1201 atctcgtcgt ccacggagaa gttcggtgcg gtcacgcgga accggtgagc ggatgccact
     1261 ttcatcttgg cgcgctcgat gccgtcgccg gatggatcct ccaagggaag gctgtcgagc
     1321 agacggagta gctctctcaa cagaaagctc ttgccgggat cggctccgcc gtcgacgact
     1381 gcagtagaga tgtgttggcg ttcgaatccc aatacgagat ctgtgtgatc gaacttggtc
     1441 tgagtgatag gtctgcttcg gtgctccgac agatcgatcc ccacccgact gctggctagt
     1501 actgcttcgg ggagtgccgg cccggcctcg agctcgagta ggcccgccga agagacttcg
     1561 accggaagat cctgggtcat cttgcggacg aatgcctcgg ccatcgcgct ccgagctcgg
     1621 ttccccgtgc acaagaagac gatctgaaac gaatctttgt ccgccatgca tgcaggctag
     1681 agcaccgcga atcccatttc tgacagagga agcagtgaca ggttcataca gaaaaaggga
     1741 atcgcgcatt ctttcggttt catcttgttt cgggggggct tgaagcccac actctccacc
     1801 acgagtcgcg gggacgtgac tcggcttggg gtgggcgagg tcggccgtcg agtgagttcc
     1861 gccaacgcac acgagccgcc tcgcccatct ataactcttc aaacgcccct taagtggacg
     1921 cgttacgcgt ttcggccagt aacggctcgt attgctcctc cgttgcgcag agtagggcga
     1981 ggtcggtcgg tgtactccgc caggacccga ccttcctcgc ccactttttc tgcctcgagt
     2041 aaggcctacc cgtttttgac tgggttagcg gaccgtcacc agcagccggc tcgggtaact
     2101 cccgttgtgg aacaggtaat tgagggccgg ctccgggagc ggctcgtggt tcggagtgtc
     2161 gctcgacgac aggctgatcc gtagccggtg gccctcgagc accgtgtgcg cgatgtccca
     2221 gatgtcgatc cggtactcga gcgtcttgcc cgacggaacc tgcgccgcac tcgtgttcga
     2281 cagccacggc ctccccggcc gagatctctt cggatcccac ttgcgatgtg atgcccttac
     2341 gtaacccgaa gtgatgagct tcgatccacc atccggcgcc acgtccgtga ctttcacgat
     2401 ccagtccgtg tcgcttgctg tggtggcccc ccagaagtgc agttccatcg gccccgctag
     2461 acgcagaggc ttcttcactg catccgtcgt gtagccgagt gaccttgcat cgtcgggtct
     2521 ctggtcggcc cggtaatgcg gtgacagcgc gaccgttccc cacttcgcga acgtctcgct
     2581 ggtaccgacc gtcgggtcgt acacgtagtg gtcttcgacg tcttggtcgt cgaccggctt
     2641 ctcgaccaac gatccgtcga cgacttcgct cgacgatccg ctcgcggccc ccgagagata
     2701 gaacgtcttc aacttcgagt cacgtggcgg ccacgcggtg tcggtcaccc attcgttcga
     2761 accgatgtcg aagaagagga ccggcggtcc atcctcgatc ccgttgtcct tgcccttgag
     2821 gaagtgctcg aaccaagcga ggtagatgtc gtctgggccg ggtgcttcgg catcggcggt
     2881 gtactcactc gccggatcga aggggccgcc acagcctttg tgggtgcacg ggaccatgat
     2941 caacctgttg tgcttgctgg cgacgcctgc gaagttatcg agcgtcccgc gcgagaaacc
     3001 gtcgaaccac gctcccatgt gcaggatcgg caccttgatc ctgtcgtact tcgtgttgac
     3061 cgatcgctcc ttgtagaagg cgtcgtccca tggctgcagg cgggactcga acaccatccg
     3121 ttgtccaacc cgtgcggcga ggtcgctcga cggcgcggtg cccgtcatcg acagcgccgt
     3181 ctgcgcggtc aaccactgtg cgacgaaatt agcgttgaag atgccgttgt gccacacgat
     3241 gtcgcggtac gcgtcggcgt acgtgacggc cgggatgatg gcttcgaggt gcggcggttg
     3301 cttcgatgca cccaggatcg gcgtgatccc ctggtacgag cctccgtaca tgccgacctt
     3361 gccgttgctc cacttctggc gcgccaccca ctcgacgacg tcgtagacgt cctgctgctc
     3421 gcggtcgcag aagatgcaca gggcaccctc cgagccaccc gtgccgcgga tgtcgacgtt
     3481 cacgtgcgcg tagccgttcc cgagataagt cgagaagtac gtcgcgggcc cgtagggcgt
     3541 catattgaga agcaccggga agcgcttgcc ctcggtcggg agcaggatct cggctttgag
     3601 gtgagccccg tcggacatgg ggatcatcgt gggggattcc tgcgccggtg cgggagacac
     3661 aagcgaggcg agcacgatcg aaaccatcgc gatgacgccg gctacccgtc gcaaagctgc
     3721 ctcccggtcg gtgagatgtc ctggtcacaa cttcgctccg ggccatgccg actcctgcag
     3781 gggcgggggg tgcgccttcg tgagaggctt ccgcaatgac acctcgcatc ccgcctacga
     3841 cggctgccga gcgcgacgag cgcaccgaag agctcctgtc gaacatgttc cggcgtcccg
     3901 acgggaccga cctcaacatc ttcaccacgc tcgctcacca ccccaagctg ctgaagaggt
     3961 gggcccagtt cggcggcacg ttgttgttcg gagggacact gtccgggcgc gatcgggaaa
     4021 tgctgatcct tcggacggca tggttgtgcg atgccgaata cgagtgggga caacacgtga
     4081 cgatcggcct caactcgggg ctcaccgaag acgagatcga gcgcatccac aaggagggcg
     4141 ccgcgggcga gtgggccgag aacgaacgag cgctcctcca agctgccgac gaacttcacg
     4201 ccgatcaccg catctccgac gagacgtgga aggtgctcga ctcggcctac gactatcagc
     4261 agctgatcga gatctgcatg gtggtcgggc agtaccacat ggtcgccttc acccttaatt
     4321 cgctcggcat cgagcccgag cccggcctgc cggagttccc caataagggt tagttcagcc
     4381 gatcttggag ctctggccgc cgtcgaccgg caggacggcg ccggtgataa acgccgcttc
     4441 gttcgaggcc aggaacaacg ctgcgttcgc cacgtcccag gcagtgcctt gttgcttctt
     4501 cagcgggacc atctcggcgc gcagcttcgc cagatcgtct cggtcgatgt cgagcgcgct
     4561 cgcgaccgca tcgaccgcca tcggagtgtc tatgaacccg ggcatgatcc cgttgactcg
     4621 gatgccgtga tgggcgttcg tgatcgctag ggaatgaacg agagcgttca cgcccgcctt
     4681 cgagatcttg taggcggtga tgtgtccggc cgccgcgatg gcggcaagtg aagacacaca
     4741 gacgatggct ccaccgttct gccggcgcat gatgggcacg acctccttgc atgtcagcca
     4801 catcgccttg aggttgacgt cgaagatccg atcccaggcc tcctccgcga gcttcgtcgg
     4861 cgtgttgtca cccatgccga tgccgacgtt gttatggagg atgtcgatcc gtccgaggat
     4921 ggctttgcct tgctccgcga tctcggcagg gccgccgggc agcgagatgt ctacaaccgc
     4981 gacgtcagct ttgcccccgt cctcgaggat cagcgcttgc gtctctttga ccgacgcgtc
     5041 atcgcgatcg acgaggagca agtgcgcgcc ctcctgcgcg aaacggagag cggtggcgcg
     5101 cccgttaccg atcgtggcac ccggcgtctg ccccgcaccc acgacgatcg ccgtctttcc
     5161 ggcgagccgc tccccagtca tcgactgagg ttattccact cggtttacgc cgacttagta
     5221 ggcgccttgc ctcttcagca atacctgcgc ggtcttgagc aagatgtaga ggtcgagcga
     5281 gagcgaccag ttctcgatgt agaagatgtc caggttcaga gccttcttgg gcgacacatc
     5341 gctgcgcccg ttgacctgcc accacccggt gatcccggaa ctgacttcgt ggcgaggcga
     5401 caggagattc tcgttggcca tgacctgatc caccggcagg ggcctcggcc cgaccaggct
     5461 catctcgccc cggatcacgt tcagcagttg gggcagctcg tcgaggctgg ttcggcgtat
     5521 gaatctgccc acccgcgtta tccgcgggtc atcctcgagc ttgaagaagg gaacgctcgg
     5581 gtcgatcccg cgctgcttca ggatctcgtc gccgtccgtc ctcatcgtcc ggaacttgaa
     5641 gacggggaag acgtgaccgt tcttggtcac cctgtgctgg cggaagaaca cgggtcccgg
     5701 cgaggtcaac ttgatggctg ccgcgatcgc ggcgaggacc ggtgtgatga agacgaggac
     5761 cacggacgac aggacgaggt cgaagatgcg cttcgtcaga gcctgtgcac ccgacaagcg
     5821 gaccggcctc aacgtcagac tcatcgtgtc tccgaccggt tggatcgtca gacgtgaagt
     5881 cagcatctcg ggcacgttcg ccgagacgcg gacatcgact cccagtcggc gacccgcctt
     5941 cgcgacgcgg agcatgtcgg tcgggcccac ggctgaagag gcaacgaata gacaatcggc
     6001 gtggttggtg aagatcacgt gatccagttc gtcgatgtgg ccgagaacgt ggatgccgtt
     6061 cgcttcgat
//
LOCUS       DATMED010000213         3057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_922601, whole genome shotgun sequence.
ACCESSION   DATMED010000213 DATMED010000000
VERSION     DATMED010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922601"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VNP73_05365"
     CDS             <1..272
                     /locus_tag="VNP73_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWL65386.1"
                     /translation="GAGFVSALIAGALAAGVLYRLTIVDGLLLGALCAIVGPAGDLVE
                     SMVKREVGTKDSSRLLPGHGGMLDRLDAIVFSAFPAYLFLRFIVF"
     gene            273..1364
                     /gene="dxr"
                     /locus_tag="VNP73_05370"
     CDS             273..1364
                     /gene="dxr"
                     /locus_tag="VNP73_05370"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWL65387.1"
                     /translation="MTSPRRIVILGSTGSIGRQALEVVRSDPQSFVVVGLAAGSDAAA
                     LDHQADEFDVKQRALGEEEAVELASLDEADVVLNAIVGAAGLRASVAALEAGKLLALA
                     NKESLVAGGVVCKSAAERGGGQIVAVDSEHAALAQCLDKRRPSEVARIVITASGGPFR
                     TRTDLEGVTPEEALRHPTWSMGPKITIDSATLMNKGLEVIEAHFLFGFEYDRIDVCVH
                     PQSVVHGMVEFVDGSLLMQAAPTDMAIPIQAALTYPDRKPGPVERIDPAKFGELTFEP
                     VDTARFPALELARSAGRSGRSFPAVLNASNEVAVGAFLDERLPFTAITGVTEKVLDKH
                     VPTEADSVEDVMAADGWARSEAEAEVGRR"
     gene            1361..2587
                     /locus_tag="VNP73_05375"
     CDS             1361..2587
                     /locus_tag="VNP73_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWL65388.1"
                     /translation="MSLGVLLFILSILIVVMIHEAGHFTVAKLFGFKATKFFVGFGPR
                     IWSVKKGETEYGIAAIPAGGFVKIVGMNPYEDVPPEDQPRSYPNKPRWQRALLLLAGS
                     ATHWPVAFLILVITAMTIGFPTGRATNEVAAIETRLQSVSGEEVAPTTPAAEAGVRPG
                     DRIVEIDGAPTPTWTAVRDTIRSHAGQTIEFVLARGDRRLTVTTDLAGVVFDDRNRIV
                     RYCPPDAPPGPLAQVCVGEDTQGELVGFFGVQPEPEYRRDGFFGAFKTAAGATAYATK
                     YAVLGVGVFFRTVFTGDLFQELTAEGDRTGREQAIGIVGAGRIASESVEKGEYIRLVE
                     LVVGFTLFVGLMNLLPLPPLDGGHLAVLAYEAITRRQVDIRKLIPVAAAVISFFVILF
                     VAVLYLDLARPIRVPF"
     gene            2602..>3057
                     /locus_tag="VNP73_05380"
     CDS             2602..>3057
                     /locus_tag="VNP73_05380"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWL65389.1"
                     /translation="MIERRKSRQIKVGSVAIGGDAPVSIQSMTITKTEDVQATLIQVH
                     ALATQGADIVRVAVPHEGDAKALPMIVQEGGVPIVADIHFSVRLAMLALEAGVHGLRL
                     NPGNMRNPKHVKEVADAAKERGIPIRVGVNAGSLDKKLIEKYKRPTADAL"
BASE COUNT          522 a          959 c         1003 g          573 t
ORIGIN      
        1 tcggggcagg gttcgtctcc gctctcatcg ccggtgcgct ggcggcgggc gtcctttacc
       61 gcctcacgat cgtcgatggg cttctcctcg gcgcactatg tgcgatcgtg ggcccggcgg
      121 gggatctcgt cgagtcgatg gtcaagcggg aagtcggcac caaggattcc agccgtctgc
      181 tacccggcca tggagggatg ctggaccgtc tcgatgccat cgttttctcg gcttttcccg
      241 cgtatctctt cctccgcttt atcgtcttct gagtgacctc accccgccgg atcgtcatcc
      301 tgggctccac cggctcgatc ggccgtcagg ccctcgaggt ggtccgctcc gatccccaga
      361 gtttcgtcgt cgtcggcctt gccgccggaa gtgatgccgc ggccctcgac caccaggccg
      421 atgagttcga cgtaaagcaa cgcgccctgg gtgaggagga agcggtcgag ctggcatcgc
      481 tggacgaagc agacgttgtg ctgaatgcga tcgtgggagc tgccgggctg cgagcctccg
      541 tggccgctct cgaggccggg aagctacttg cccttgccaa caaggaaagc ctcgtggccg
      601 gcggcgtcgt gtgcaagtcg gcggccgaac ggggcggggg gcagatcgtt gccgtggatt
      661 cggaacatgc tgctctcgct cagtgtttgg acaaacgtcg gccctcggag gtggctcgca
      721 tcgtcatcac ggcttcgggg ggccctttca ggacccggac cgatctcgag ggagtgacac
      781 cagaagaagc cctgcgccat cccacgtggt cgatgggtcc caagatcacg atcgactcgg
      841 caacattgat gaacaaaggt ctggaagtga tcgaggcaca tttcttgttc ggattcgaat
      901 acgaccgcat cgacgtttgt gtccaccccc agagtgtcgt ccacgggatg gtcgagttcg
      961 tcgacggctc tctgttgatg caagctgctc ccaccgatat ggccatcccg atccaagccg
     1021 cactgactta ccccgacagg aaacccggtc cggtcgaacg gatcgatcct gcgaagttcg
     1081 gcgagttgac gtttgagcct gtcgacaccg ctcgtttccc agcgctcgag ctcgcgcgct
     1141 cggcgggacg atcgggccgt tcgttcccgg ccgtcctgaa cgcctcgaac gaggtcgccg
     1201 tgggcgcatt cctcgacgaa cggctgccgt tcactgccat caccggcgtg acggagaagg
     1261 tgctcgacaa gcacgtcccg acggaagccg attccgtcga ggacgtgatg gcggctgatg
     1321 gatgggcgcg cagcgaggcc gaggccgagg tgggtcgacg atgagcctgg gcgtcctgct
     1381 cttcatcctg tcgatcctca tcgtggtgat gatccacgag gccggccact tcaccgtggc
     1441 caagttgttc ggcttcaagg cgacgaagtt cttcgtgggg ttcggccccc gcatctggtc
     1501 ggtcaagaaa ggcgagaccg aatacgggat cgcggcgatc ccggccggag gcttcgtgaa
     1561 gatcgtcggc atgaaccctt acgaagacgt gcctccggaa gaccaaccgc gctcgtaccc
     1621 gaacaagccg cgctggcagc gagcgcttct tctgctggcc ggctcggcaa ctcactggcc
     1681 ggttgccttc ttgatcctgg tgatcaccgc gatgacgatc gggttcccca ctggtcgtgc
     1741 cacgaacgag gtggccgcga tcgaaacccg tctgcagtcc gtttcgggag aagaggtggc
     1801 cccgacgacg ccggcggctg aagccggtgt gcgtccgggt gaccggatcg tcgagatcga
     1861 tggggccccg actccgacct ggacggccgt cagagacacg atccggtccc acgcaggaca
     1921 gacgatcgag ttcgtcctgg ccagaggtga ccgtcgactg acggtgacta ccgatcttgc
     1981 cggtgtcgtg ttcgacgatc gcaaccggat cgtccgctac tgcccaccgg atgccccgcc
     2041 gggtccccta gcccaggtat gcgtggggga ggacacacag ggtgagctgg tgggcttctt
     2101 cggcgtgcag cccgagcccg aataccggcg ggacggtttc ttcggagctt tcaagacggc
     2161 ggccggcgcc accgcttacg cgaccaagta cgcggtgctg ggagtcggtg tcttcttcag
     2221 gaccgtgttc accggggatc tctttcagga actgacggcc gaaggtgatc ggacgggacg
     2281 cgagcaggcg atcggcatcg tcggagcggg ccggatcgca agcgagtcgg tggagaaggg
     2341 cgagtacatc cgcctggtcg aattggtcgt gggtttcacg ttgttcgtag ggctcatgaa
     2401 cctgttgccc ctgcccccac tcgacggagg acatctggcg gtgctcgctt acgaagcgat
     2461 cacccggcgc caggtggata tcaggaagtt gataccggtc gcggcggcgg tcatctcgtt
     2521 cttcgtgatc ctcttcgtag cggtccttta cctggacctt gcgcgcccga tacgcgtccc
     2581 cttctaacta gactggatgg catgatcgag cgacgcaagt ccaggcagat caaggtcggt
     2641 tcggttgcga tcggcggcga tgccccggtc tcgatccagt cgatgaccat caccaagacc
     2701 gaggacgtcc aggccaccct gatccaggtc cacgcgctcg cgacgcaggg cgcagacatc
     2761 gtccgggttg ccgtgcccca cgaaggagac gccaaggcgc tgccgatgat cgtccaggag
     2821 ggcggcgtcc cgatcgtggc cgatatccac ttctcggtcc gtctcgcgat gctcgcgctc
     2881 gaggccggag tgcacgggct ccgcctcaac cccggaaaca tgcgcaaccc gaaacacgtg
     2941 aaggaggtcg ccgacgctgc gaaggagcga ggcatcccga tccgggtggg ggtaaatgcc
     3001 ggctcgctcg acaagaagct catcgagaaa tacaagcggc ctaccgcgga cgcactc
//
LOCUS       DATMED010000214         5633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3613765, whole genome shotgun sequence.
ACCESSION   DATMED010000214 DATMED010000000
VERSION     DATMED010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3613765"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VNP73_05385"
     CDS             complement(<1..233)
                     /locus_tag="VNP73_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWL65390.1"
                     /translation="MVAGQNRSLAQMRKGTLEYCVLALLDEEERYGFDLVKSLSDVDG
                     MLTAQGTIYPLLTRLRRDGLVSSNWQESAEGPPR"
     gene            complement(326..1423)
                     /locus_tag="VNP73_05390"
     CDS             complement(326..1423)
                     /locus_tag="VNP73_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWL65391.1"
                     /translation="MPDAKGPPQRPELMIAGPGELHDADLEVYGRQVTAHYGDVWTEQ
                     HAQTIDMLGDFLGAADPPYLIPGTGTTCLDAGLFNLFEAGQKVVVANTGFFGMRLMQM
                     AQAHRLSVVEVPVEAGQPADPDALADAAKDADGILTVHVDTATGVRHPIEEIAAVAQE
                     TGALYMVDGIASVGGETLKVDEMGIDCVVTGTQKGFEAPPGLGILALGARGRNRVMSR
                     SERPDSWYLDLQTWDWYRKEWGAWHPHPVTMPTNLVLALRSSMSRILEAGLESWIAGR
                     ADLAKRCREGLRDLGLEPVPAAGHEANLVVAAWADDPAAIQKYLLDHGIMISGGLTPL
                     QGRTIRVGLMGKTATPEMVDRVLNLVGAVLG"
     gene            1525..2700
                     /locus_tag="VNP73_05395"
                     /pseudo
     CDS             1525..2700
                     /locus_tag="VNP73_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            complement(2781..3866)
                     /locus_tag="VNP73_05400"
     CDS             complement(2781..3866)
                     /locus_tag="VNP73_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWL65392.1"
                     /translation="MSPGTLDPWRAWHDDLLDNSLIHADAAGCSARSRRVTAAVKEHL
                     DLEATNGAYIAEEMAADRIEAARAGLSRLAGGGRVAFAQNASQAFRWLISAWPLPPGA
                     SVGTVPGEYPANAATLEMGAVWRDLRLVQLPTDPLGYIDIEGAEKLLPELDLIAFPHI
                     PSHRGIAQPADAIAELCRAHGVDLILDAAQSFGQQDVSRIRPSAFVGTSRKWLQGPRG
                     VGFVVVADGRIELDGPTGPEEGDAGVAERVGLATAVAELERRGIDAVAARISELVTRL
                     REGLTEKQGWNVLEPSATVAGSTTLRHPDHDPVAMVHHLRTCGVIASAIPVARAPNEL
                     DAPLLRFSLHAYSDGDWVDRIVEAIPA"
     gene            complement(3863..4828)
                     /gene="egtD"
                     /locus_tag="VNP73_05405"
     CDS             complement(3863..4828)
                     /gene="egtD"
                     /locus_tag="VNP73_05405"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWL65393.1"
                     /translation="MTYTIDVHVGPDHVMRSLRNDVATGLTREPKQLPPKWFYDDRGS
                     QLFDAITRLPEYYLTSRESEILSARSAEIAALTEADTLVELGSGTSAKTRMLLDALAR
                     AGRLRLFVPFDVSEGVLREAAHSIGRAFPTVDVHAVAGDFDLHLQHLPKGGRRLVIFL
                     GSTIGNLDPAARKAFFDEIAGDLDPGDHFLVGADLVKDVAILEAAYNDPSGVTEAFNK
                     NVLSVINRELHADFDIDRFAHLAFFDADNSWIEMRLISQVAHPVAIPDLDLEIELAKD
                     EYITTEISTKFVLEEISDEIAATGMTVRRAWTDERADFALLLAAR"
     gene            complement(4825..5580)
                     /gene="egtC"
                     /locus_tag="VNP73_05410"
     CDS             complement(4825..5580)
                     /gene="egtC"
                     /locus_tag="VNP73_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HWL65394.1"
                     /translation="MCRFLAYLGPSRTLDTLLIEPQHSLYRQSWEPQNQKHGTINADG
                     FGVGWYEPSLRPEPALYRSARPIWADRSFASMAGVLASGAVLAAVRSATEPAILEESN
                     VPPFVSGRYLFAHNGAIEGFRDGVASKLRRGLSEERDSGILGTTDSEVLFALTLDELD
                     GGASLADALAAVVARVSAVARCRLNMIVTDGGRIAATSFGDDLFVGDEGDSVFVCSEP
                     FDDSSTWKQIDDRMVLEAGIGEVSVSPLSRGDS"
BASE COUNT         1027 a         1781 c         1847 g          978 t
ORIGIN      
        1 cgagggggcc cctcggccga ctcctgccag tttgaagaga ccaggccgtc gcgtcggagc
       61 cgggtgagca gcggatagat cgttccctga gcagtgagca tcccgtcgac gtcgctcagc
      121 gacttcacca gatcgaaccc gtatcgctcc tcctcatcga ggagcgccag aacgcagtat
      181 tcgagggtgc ccttgcgcat ctgggccagc gaccggttct gaccagctac catgtattac
      241 atagtagctg gttttgcata actccaccag tcgtcgcgat tttcttctaa cttgtcgcgt
      301 cagagcgcgc tgaagggtta ctcagttagc caagaacggc gccgaccagg ttcagaaccc
      361 ggtcgaccat ctccggagtg gcggtctttc ccatcaggcc gacacggatg gtgcggccct
      421 gaaggggggt cagaccgccg gagatcatga tcccgtggtc cagcaggtac ttctggatcg
      481 ccgcggggtc gtcggcccag gcggccacca cgaggttcgc ttcgtgtccc gccgcgggca
      541 ccggttctag acccaggtct cgcaagccct cacgacagcg cttggccagg tcggcccgcc
      601 ctgcgatcca cgattcgaga ccggcctcga ggatgcggga catgctggaa cgaagtgcga
      661 ggacgagatt cgtcggcatg gtcacggggt gcggatgcca tgcaccccac tccttgcggt
      721 accagtccca cgtctggaga tcgagatacc aggaatcggg gcgctcgctt cgggacatga
      781 cacggttgcg tccccgggcc cccagggcaa ggatgccgag gccgggtggc gcctcgaacc
      841 ccttttgcgt accggtgacc acgcagtcga tccccatctc gtcgaccttc agggtctcgc
      901 ccccgacgga tgcgatgccg tcgaccatgt agagcgcgcc ggtctcctgc gcgaccgcgg
      961 cgatctcttc gatgggatga cggacgccgg ttgccgtatc gacgtgaacc gtgagtatgc
     1021 cgtcggcatc tttggccgca tccgccaatg catcggggtc ggcgggttga cctgcttcga
     1081 ccgggacctc gacgacagac aagcgatgtg cttgcgccat ctgcatcaac cgcatgccga
     1141 agaagcccgt gttcgcaacg acgaccttct ggccggcttc gaacagattg aagagaccgg
     1201 catcgagaca tgtggtgccc gtcccgggga tcagatacgg cggatcggcc gctcccagga
     1261 agtcgccgag catgtcgatc gtctgggcat gttgctcggt ccagacgtcg ccgtaatgag
     1321 ccgtaacctg ccgcccataa acctcgagat ccgcgtcgtg caactcgccc gggcccgcga
     1381 tcatcaattc gggcctttgc ggaggcccct ttgcgtccgg catggtccga tgctacggca
     1441 caggcatccg gtagcgtgag ggtggacgaa aggagatctg tgactcggtt gccccgactc
     1501 acactgggac tcaccgtgct actgatgggc ggcttcgccg tcaccggtgt cgcggctaac
     1561 gctcatgcgc agaccgcacg ctgcctcggt cgcagggcga cgatcgtcgg gtcaaaccgc
     1621 accgaacgga tcttggggac gccccaacag gatgtgatcg ttgccaaggg cggagccgat
     1681 ctcgttcggg cagggggagg gcccgacgtg atctgcgggg gtccgggcgg ggatgccctt
     1741 tatggatcgg gaggcaacga cgacatccgc ggcgtggacg gctcggatgc gctgttcggg
     1801 ggtaacggcc gggaccagct cgtggtgggt ctgggccgcg acgagttcgt ggtgggcggt
     1861 cccggcaacg accgtctggt cggcaacgac accctcaccc tggcgagcta ccgaggcgcc
     1921 tcgggggcgg tgaacgtgga ccttttcaat ggcgtgacca gcggggccga cggtacggac
     1981 acgcttctgg acatagccgc cgtccagggt tcgcccttcg acgacagcat cacaggcgat
     2041 tcgtcgacga actacctctt cgggggcaag ggcaacgacg cgatcgccag cggcagcaat
     2101 tctggaagct tcgaggaggt cggcgccacc ggccccaacc agttcttcga attcgacttc
     2161 gtcgtcggag agggtggcga cgacacgatc accggaggat ccggtctcga ggtcgccgat
     2221 tacacgatgt cggattcccc agtcgatgtg aatctcacga cgggtgtggc gaccggtgat
     2281 gggaacgaca tcctgtcggg gatcaacgta gtgatcggtt cgttcttcga cgacgtgctc
     2341 actggtaacg acgaagacaa cggcttcgag ggtgggggcg gtgcggacga gatcgaaggc
     2401 aagaacggca atgacgttgc catctttttc gccgcgcaac ctggggtcga cctgaacctc
     2461 gcggagggaa gcgcgacggc tcagtacttg cgcgcgagca gacacgcggt cgagatcgat
     2521 gagtcgacgg acgcactgac gtcgatcgac gacggatggg gctcggaagg tgacgactcc
     2581 atggtcggcg accagttcga gaaccggttc ttcggattcg cgggcaacga ttccttccag
     2641 ggtgacgccg gtgacgacat cttgagtggc gcacgcgggg ccgacgacgc cgacggcggt
     2701 gacggcaacg acgcctgcga ggccgagcaa gagatcaatt gcgaagcgga accatcggct
     2761 gcggctcgcg cgctcggcgc ctacgcggga atggcctcaa cgatgcgatc gacccagtcc
     2821 ccgtccgagt acgcgtggag cgagaagcgc aacagcggag cgtccagttc gttcggcgca
     2881 cgagcgacgg gtatggccga ggcgatgacg ccgcacgtcc ttaggtggtg gaccatcgca
     2941 accggatcgt gatcgggatg acggagagtg gtgcttcccg ccaccgtcgc ggatggctcg
     3001 agcacattcc atccttgctt ctcggttaag ccctcacgca agcgcgtaac caactcggag
     3061 atcctggccg cgacggcgtc gatcccccgg cgttcgagct ccgccaccgc agtcgccaga
     3121 ccgactctct cggcgacacc cgcgtctccc tcctcgggcc cagtagggcc atctagttcg
     3181 atccgaccgt cggccacgac cacgaagccc acgccacggg ggccctgcaa ccacttccgg
     3241 gacgttccga cgaaggccga cggacggatg cgggatacat cttgttgtcc gaaggactga
     3301 gcggcgtcga ggatcagatc gaccccgtgg gcgcggcaca gctccgcgat cgcatcagcc
     3361 ggttgagcga tcccacggtg actcgggatg tgagggaagg caatgaggtc gagctcggga
     3421 agaagtttct cggccccctc gatatcgatg taaccgagcg ggtcggtggg cagctgcacg
     3481 agccggagat cacgccagac cgctcccatc tccagggtcg ccgcgttcgc cggatactcg
     3541 ccggggaccg tcccgaccga agctccgggg ggcaacggcc aggccgagat cagccaccgg
     3601 aacgcttgcg aggcgttctg ggcgaaggcg acgcggcctc cacccgccaa ccgtgacaga
     3661 ccggctcggg cggcttcgat ccgatcggcc gccatctcct ccgcgatgta agcgccgtta
     3721 gtcgcctcga gatcgagatg ctctttcact gcggccgtga cgcgtcgcga tcgggccgag
     3781 caaccggctg catcggcatg gatcagtgag ttatcgagga gatcgtcgtg ccatgcgcgc
     3841 cacggatcga gggtgcccgg gctcacctcg ccgcgaggag cagcgcgaag tccgctcgct
     3901 cgtccgtcca cgcccttcgc acggtcattc ccgtcgccgc gatctcgtcc gagatctctt
     3961 cgagaacgaa cttcgtactg atctcggtcg tgatgtattc gtccttcgcg agctcaatct
     4021 cgaggtcgag atctgggatc gcaacgggat gagctacctg agatatgagc cgcatctcga
     4081 tccatgagtt gtcggcgtcg aagaaagcca gatgtgcgaa ccggtcgatg tcgaagtctg
     4141 cgtgcagttc tctattgatc acgctcaata cattcttgtt gaacgcctcg gtgacaccac
     4201 tcggatcgtt gtaagcggct tcgaggatcg cgacgtcttt cacgagatcg gctccgacca
     4261 ggaagtgatc tccggggtct agatcgcctg cgatctcgtc gaagaatgcc ttgcgcgccg
     4321 cgggatcgag gttcccgatc gtgcttccga ggaagatgac gagtctgcgt cctcccttgg
     4381 gcaggtgctg gagatgcaga tcgaagtcac ccgcaactgc atgcacgtcg acggtgggga
     4441 aagcgcggcc gatcgaatga gccgcttcgc gcaggacccc ctcacttacg tcgaacggga
     4501 cgaatagtcg cagccggccc gcccgtgcga gcgcgtcgag cagcatcctc gtcttggccg
     4561 aggttccaga accgagctcg acgagtgtgt cggcttccgt caacgccgcg atctcggccg
     4621 agcgagcact caggatctcg ctctcacggc tggtcaggta gtactccggg aggcgggtga
     4681 tggcgtcgaa caactgggag ccccggtcgt cgtagaacca tttcggtggc agttgcttgg
     4741 gctctctcgt aagcccagtc gcgacgtcat tgcgcagcga gcgcatcacg tgatccggcc
     4801 cgacgtgaac gtcgatcgtg taggtcatga gtcgcctcga gacagcggtg acaccgacac
     4861 ctctccgatc cctgcttcga ggaccatccg gtcgtcgatc tgcttccacg tggacgagtc
     4921 gtcgaacggc tcggaacaga cgaagaccga gtcaccctcg tccccgacga acagatcgtc
     4981 accgaagctc gttgccgcga tgcgaccgcc gtcggtgacg atcatgttca gccgacagcg
     5041 cgcgaccgca gagaccctgg ccacgacggc ggccagcgca tcggcgagcg aagctccccc
     5101 atcgagctca tccagagtga gtgcgaacag aacctcgctg tcggtggttc cgaggatccc
     5161 cgagtcgcgc tcctcgctga gaccgcgccg aagcttgctc gcgaccccgt cgcggaagcc
     5221 ttcgatcgca ccgttgtggg cgaagaggta ccggcccgat acgaacggcg ggacgttgga
     5281 ttcctcgagg atcgccggct cggtcgcact acggaccgcg gcaagaacgg cgcccgatgc
     5341 cagaacgccg gccatgctgg cgaacgaacg atccgcccag atcggccgcg cgctgcggta
     5401 gagagcgggc tccggccgca gagagggttc gtaccaaccg accccaaagc cgtcggcgtt
     5461 gatcgttccg tgtttctggt tctgcggctc ccacgactgc cggtagagcg agtgctgcgg
     5521 ctcgatcagc agagtgtcaa gggtccggga cggaccgagg tacgcaagga agcgacacac
     5581 tcaggcgtcc ctcgcgcatc tgaagccggc gaagatctgg cgacggatcg ggt
//
LOCUS       DATMED010000215         5499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2100909, whole genome shotgun sequence.
ACCESSION   DATMED010000215 DATMED010000000
VERSION     DATMED010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100909"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VNP73_05415"
     CDS             complement(<1..759)
                     /locus_tag="VNP73_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWL65395.1"
                     /translation="MKLRVVNEETGEFEVGHISDVVDKGIQPVYLITLADGKQLKATE
                     NHQMFTSEGWRTLREAVGLEGSGSDTVVTRECALMTNSRAAYQDPQWMKARRIAGQSV
                     QQIADEAGCSYMTIRKWLKRHDMQFSPSERNFKKGHRPWNKDRKGYKVNRRWTAEHKE
                     AIRKARSGPNSNFWRGGISSERANIARWSREQAPKVHLQYDYTCQACGERGGKLHAHH
                     IIPVWLDPLRARDIGNLISVCDVCHRSIHATLASE"
     gene            complement(1125..1328)
                     /locus_tag="VNP73_05420"
     CDS             complement(1125..1328)
                     /locus_tag="VNP73_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65396.1"
                     /translation="MPEVITPGDKPNELKLFEHGFVRLDSAMADDLSVVNSARVSFAV
                     RKEVMEEKDRGLIRFLMRDRHGT"
     gene            complement(1357..2373)
                     /locus_tag="VNP73_05425"
     CDS             complement(1357..2373)
                     /locus_tag="VNP73_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL65397.1"
                     /translation="MPREHRRREPAPGIFRLVLPLPVPELDRVNAYLLVGDEPILVDC
                     GMYSPDEAKDHGWSEIEAAFAAHGMSPGDVKHLLITHPHIDHYGMAGRFVAETGCDLW
                     MHQEAESDLEMYRNPRAVVDQLRAMFKDHGVSGDALDELLEYEDVRILISDVIEPDRA
                     LKGGERFKVGDREWEVVFTPGHSRAHVCPWSADDRILISGDHLLPTITPHIDFKRGED
                     EDPLGDFLESLAKVERLDPSLVLPGHGHPFEEGAERARIIERHHDRRLGSIVQVIRNE
                     PQTAEQITNEIFGTELLHFQKRLALGEALAHLAYLRQRGEVERIKNQDGVYVYKKVSR
                     KQQS"
     gene            complement(2382..2591)
                     /locus_tag="VNP73_05430"
     CDS             complement(2382..2591)
                     /locus_tag="VNP73_05430"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWL65398.1"
                     /translation="MAVEKSIDLTATGATIEEAVAEAVHRASLTLKGLTSFEVERIEG
                     VIENGEVAYRVLVRIAFQIKEQIHE"
     gene            complement(2651..2842)
                     /locus_tag="VNP73_05435"
     CDS             complement(2651..2842)
                     /locus_tag="VNP73_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65399.1"
                     /translation="MSTERCNWMSGPKDATVRVSAVKWWLAISRDELVNDYRRRSSKN
                     RPPVTCHRTPSFTSPFGSR"
     gene            3046..3555
                     /locus_tag="VNP73_05440"
     CDS             3046..3555
                     /locus_tag="VNP73_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65400.1"
                     /translation="MKSPAVRILLALALLVGLPGAPAVAAPIDACAMTSQRLVYVYSL
                     HIEAKPGKKAYRRGDKVKVLIRVTRPAEEDPAGQGQQLPPTVSEPAEGATVGGIIWVG
                     DDYSWDQGAITDANGEAVLNIPTRKNFDVGMARADFVAEIVHYNNNGCPDVRELGYRS
                     YENFVRITP"
     gene            complement(3545..4144)
                     /locus_tag="VNP73_05445"
     CDS             complement(3545..4144)
                     /locus_tag="VNP73_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65401.1"
                     /translation="MATSKAFLLAVLLVVSVAAPAVAKCCPLDHLVIKTPGGGQLHWI
                     DGRDIEKRLPDWARGLLTWPVFYGVGKGNEPPEGKLGRSYLAIYVHEAVEGGRVVRAR
                     ERIYPAAEGGPIAFTLPGQTQEFFPGDERRVPAGWRPVEEEVWLAIVESDPVKPLGPL
                     TPLPAREGGVRKFAVGVVRTLTVALLLLALGWKMKTARA"
     gene            complement(4162..5394)
                     /locus_tag="VNP73_05450"
     CDS             complement(4162..5394)
                     /locus_tag="VNP73_05450"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWL65402.1"
                     /translation="MTPFEAATYFFYEAADRLNLDNEMREILASSYRELAVQVPVVMD
                     DGHRMVFRGYRIQHNGARGPYKGGIRYHESADLDEVRALAALMTWKNALIDVPFGGAK
                     GGVQCDPRSMSDGELQKLTRRFTAMISYVIGLNRDIPAPDMGTNAQTMAWMMDAYGQK
                     YGYTPGIVTGKPVEMGGSPGREEATGRGVVMSTKEACERKGTKFKGASIAIQGFGNVG
                     YWAAIIAEQEGAKVVAVSDVEGGIFNGDGLEIEKVDKHRWNEGSVTSYTEGDKISNED
                     LLEVECDILIPAAIQGVIHERNAEQIKAPMIVEAANGPTTPVADNILSKRDITVLPDI
                     LVNAGGVTVSYFEWVQNIQQFRWEIDNVNKELEKRMIKATDDVFARAAKDQTTLRDAA
                     FDIAVERVARAAEIRGYV"
BASE COUNT         1005 a         1746 c         1593 g         1155 t
ORIGIN      
        1 ctcactcgcc agagtcgcgt gtatcgagcg gtggcagaca tcacaaaccg aaattaggtt
       61 tccgatatcc cgggccctca agggatcgag ccagacagga atgatgtggt gggcgtgcaa
      121 tttgccacct ctttcgccac aagcctggca ggtgtagtcg tattgaaggt gaaccttggg
      181 cgcctgttcc cggctccagc gagcgatgtt ggcgcgctca gaagaaatcc cccctcgcca
      241 gaagttgctg ttggggcccg accgggcttt gcgaatcgct tccttatgtt cggccgtcca
      301 gcgccgattg accttgtacc ccttgcggtc tttgttccac ggcctgtggc ccttcttgaa
      361 gttgcgctct gaggggctga attgcatgtc atgcctcttt agccacttcc ggatagtcat
      421 gtagctgcag ccagcttcgt ctgcaatctg ctgaacactc tggcccgcaa tacgacgagc
      481 cttcatccat tgagggtcct gataggcagc acgactgttc gtcatgagcg cgcattctcg
      541 tgtcacgacg gtgtcagaac cagagccttc caggccaacc gcttccctca gtgttcgcca
      601 gccttccgag gtaaacatct ggtgattctc ggtagctttc agctgcttgc cgtccgccag
      661 cgtgatcagg tagacgggtt gaatcccctt gtccaccaca tcggagatgt ggccgacttc
      721 gaactcacct gtctcctcat tcaccacccg cagtttcata ttccggatcc gagatctcgc
      781 actgcggaaa ccggttggat tcccagccag atgtcgtcct atcgcacgac ggcctcgacc
      841 catggtcctg gagatcgcac gagcactgag cccttgatcc gccagccgat tgatctgtct
      901 cacctcgctg ggagtcaatg cgtgaccgtc gatttcgccg acactccaca tctcccagag
      961 ttcatccatc gtcttggcca ggtctcggtt ggctacccca tttgtgtcga cgaatgtgac
     1021 tagggcatcg cccgttaggc acccgattcg gtggcgaaac cattcgcgcg cgacaaacat
     1081 cggagtacgg atgtggaagc gaaaggagtt gtgttcaaag ggcgtcacgt gccgtggcga
     1141 tcacgcatca gaaagcggat caagccacgg tctttttctt ccatcacctc cttgcgcacg
     1201 gcaaaggaca cacgtgcgga gttgacgacg gataggtcat cggccatggc cgaatcgagg
     1261 cgcacgaagc cgtgttcgaa gagcttcagt tcgttcggct tatcgccggg agtgatcact
     1321 tcaggcatag tcgcgcgagt ctatagagag gcagtgtcag gactgctgtt ttcgcgacac
     1381 cttcttgtag acataaacgc cgtcctggtt cttgatccgt tcgacctcac cccgttgtcg
     1441 caggtaagcg aggtgggcga gagcttctcc cagcgccagc ctcttctgga agtgcaatag
     1501 ttcggtcccg aagatctcgt tggtgatctg ttcggcggtc tggggctcat tgcggatcac
     1561 ctggactatc gagcccagcc tgcggtcgtg gtgtcgttcg atgatccggg ctcgctcggc
     1621 cccctcttcg aacggatggc catggccggg caacaccaaa gacgggtcga gccgctcgac
     1681 tttcgccagg gactcgagga agtcccccaa gggatcctcg tcctcgcccc tcttgaagtc
     1741 gatgtgcgga gtgatcgtcg gcagcaggtg gtcgcccgag atcaagatcc ggtcgtccgc
     1801 ggaccacgga cagacgtgag ctcgggaatg gccgggggtg aacacgacct cccattccct
     1861 gtcccccacc ttgaagcgct ctcccccctt aagggctcgg tcgggctcga tcacatccga
     1921 gatgaggatc cgcacgtcct cgtattcaag gagctcatcc agggcatctc cacttactcc
     1981 gtgatccttg aacatggctc gcagttgatc gacgaccgcc cgggggttcc gatacatctc
     2041 gaggtcggat tcggcttctt ggtgcatcca tagatcgcat ccggtctcgg cgacgaaccg
     2101 ccctgccatc ccgtagtggt cgatatgcgg atgggtgatc agcaggtgtt tgacatcgcc
     2161 cggcgacatc ccatgcgccg cgaacgcagc ctcgatctcc gaccatccgt gatccttcgc
     2221 ctcgtccggc gagtacatcc cgcaatcgac caggatcggc tcgtcgccaa ccaagagata
     2281 ggcgttcacc cggtcgagtt cggggaccgg caacggaagg acgaggcgga atatcccggg
     2341 ggccggctct cgccggcgat gctcacgcgg catgcgaacc actactcgtg gatctgttct
     2401 ttgatctgga aggcgatgcg gacgagaacg cggtacgcga cttctccgtt ctcgatcacg
     2461 ccctcgatgc gctcgacctc aaaggacgtc agccccttca gggtgagact cgcacggtgg
     2521 accgcctccg ccaccgcctc ctcgatcgtg gccccggtcg cagtgaggtc gatcgacttc
     2581 tcgacggcca tcagaaaaaa cccccttcgc tgtaagcaaa gggggccagt ctaggtaaag
     2641 agctgaggcg ttaccgggat ccgaacggag aggtgaagct cggggtcctg tggcacgtca
     2701 ccgggggcct gttcttgctg ctgcggcgcc ggtaatcgtt gacaagttcg tcccgggaga
     2761 tggcgagcca ccacttaacg gcagagacac gcactgttgc gtccttcggc ccggacatcc
     2821 agttgcatct ctcggtgctc atccgctact ccttgtcgag ttacttgctt cggttgttcc
     2881 cagagttcgg gagtccaatc tcattcgctt gactttttga ttcgtcggac gggtatcccc
     2941 tcctgccccg tgcggaaaaa aagtgcctct gagcagccaa aacgagtcat atgtccgaaa
     3001 taggtggccg caggagagag ctttttttca gcgaactttc ccttcatgaa gagccccgcg
     3061 gttcggatct tgctggcgct tgcgttgctt gtgggattgc ccggcgcccc cgcggtcgcc
     3121 gcgcccatcg atgcatgcgc gatgacgtcg cagcgcctcg tctacgtgta ctcgcttcac
     3181 atcgaggcga agccggggaa gaaggcatac cggcgcgggg acaaggtcaa ggtcctaatc
     3241 agggtgacac gccccgctga ggaagatccg gccggccaag gccagcagtt gcctccgacg
     3301 gtcagcgaac ccgcggaggg tgcaaccgta ggagggatca tttgggtcgg agacgactac
     3361 tcatgggatc agggggcgat caccgacgcc aacggagagg cggtactgaa catccccacc
     3421 cgcaagaact tcgacgtggg tatggctcgc gccgatttcg ttgcggagat cgttcactac
     3481 aacaacaacg gctgccccga cgtccgagag ctcggctacc ggagctacga gaatttcgta
     3541 aggatcacgc cctagcagtc ttcatcttcc agccgagcgc aagcagcaac aacgcgaccg
     3601 tcagtgtcct aacgaccccg acggcgaact tccttacgcc cccctcccga gccgggagag
     3661 gtgtgagtgg gccgagcggc ttcacgggat cgctctccac gatggccagc cagacctcct
     3721 cttcgactgg ccgccacccc gcaggaaccc gacgttcatc gccgggaaag aactcttgag
     3781 tctgcccggg caatgtgaac gcgatcggac ctccctcggc tgcgggatag atgcgttcgc
     3841 gcgctctaac cacgcgcccc ccttcgacag cctcgtgtac atagatcgca agataggagc
     3901 gccccaactt tccttccgga ggttcgttgc ccttccccac cccatagaag acgggccagg
     3961 tcaatagccc tcgagcccag tccggcaatc gcttctcgat atcgcggccg tcgatccaat
     4021 gcagttgacc acccccgggc gtcttgataa cgagatgatc gagggggcaa cacttcgcta
     4081 cggcgggtgc ggcaaccgat acgactagaa gaacagcgag caggaacgcc ttagacgtag
     4141 ccatcaggcc ggcgactacg tctagacgta gccgcgtatc tcggcggctc gagctactcg
     4201 ctccactgcg atgtcgaagg ctgcatcccg cagcgtggtc tgatccttgg ccgcgcgggc
     4261 gaacacatcg tccgtggctt tgatcatgcg tttctcgagt tccttgttga cgttgtcgat
     4321 ctcccagcgg aattgctgga tgttctggac ccactcgaaa tacgagaccg tcactccccc
     4381 cgcgttgacg aggatgtcgg gcagcacggt gatgtcgcgt ttgctaagga tgttgtcggc
     4441 gaccggcgtc gtggggccgt tggctgcctc gacgatcatc ggggccttga tctgctcggc
     4501 gttccgctcg tggatgacgc cctggatcgc cgccggtatc aggatgtcgc actcgacctc
     4561 gagcagatcc tcgttactta tcttgtcgcc ctcggtgtag ctcgtgacgc tgccctcgtt
     4621 ccaccgatgc ttgtcgacct tctcgatctc gaggccatca ccgttgaaga tgccgccttc
     4681 gacatcggaa accgcaacga ccttcgctcc ttcctgctcg gcgatgatcg ccgcccagta
     4741 acccacgttg ccgaagccct ggatcgcgat gctggccccc ttgaacttcg tccctttcct
     4801 ctcacacgct tctttggttg acatcacgac gccgcggccg gttgcctcct cgcgtccggg
     4861 cgatccaccc atctccacgg gtttccccgt aacgatgcct ggcgtgtagc cgtacttctg
     4921 gccgtacgcg tccatcatcc aggccatcgt ctgcgcgttc gttcccatgt cgggggcggg
     4981 gatgtctcgg ttcaggccta tgacgtaaga gatcatcgcg gtgaaccgcc gggtcagctt
     5041 ctgcaactcg ccatctgaca tcgagcgcgg gtcgcactgc actccgccct tggccccccc
     5101 gaagggaacg tcgatcaatg cgttcttcca cgtcatgagt gccgcgagag cgcgcacctc
     5161 atcgaggtcg gccgactcgt ggtaacggat gccgcccttg tacggccccc gcgcgccgtt
     5221 gtgctggatg cggtagcccc gaaacaccat ccggtgaccg tcgtccatca ccactggtac
     5281 ctgcaccgcg agctcccggt acgaagaggc caggatctcg cgcatctcgt tgtcgaggtt
     5341 caagcgatcg gcggcctcgt agaagaagta ggtggcggcc tcgaacggag tcatgtcgtc
     5401 cgaaggctcc gtgacgcgtt tgggcgcgcc tatgtcctcg gcggaaccga tcgggcgttt
     5461 ctcgtcgttg gcagcgccca cgacgatgag attaaccct
//
LOCUS       DATMED010000216         5475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2101155, whole genome shotgun sequence.
ACCESSION   DATMED010000216 DATMED010000000
VERSION     DATMED010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101155"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VNP73_05455"
     CDS             <1..404
                     /locus_tag="VNP73_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWL65403.1"
                     /translation="SHVSTDLVLLTIAAGVMVGVLSALLGVGGGLLMVPFMVLLLGET
                     QHVAEGTSLAVIVPTAIVGVIALSRRGFVNFVGAGLLAVGGIFGAGLGARLALNLDGD
                     VLKTVFGAVVIVMGARFVHQGLTTDPTAEPH"
     gene            complement(419..814)
                     /locus_tag="VNP73_05460"
     CDS             complement(419..814)
                     /locus_tag="VNP73_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HWL65404.1"
                     /translation="MAQHQIDPDNLPHAPEKLPKKEDIEATDFFALDLRTGRVVSVED
                     FPEARKPAYKLTVDFGPTVGRLTTSAQITNYSKEELDGRIVVGAINLGTKKIAGFSSQ
                     FLILGSLDPDGTVRLLNLEDGVGPGSPIA"
     gene            855..1394
                     /locus_tag="VNP73_05465"
     CDS             855..1394
                     /locus_tag="VNP73_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL65405.1"
                     /translation="MRRTGSLGVVVALFLLVHPGVPGAQEETRRCDGATATIVGTAGN
                     DQLTGTSGDDVIVGLGGNDDIQTSGGTDRVCGNRGSDVIVRTEDTDPADPDIGRFFGG
                     RGADRAEGGNSPNDQQVFDTLHGGPGNDQVLGHGGNDQVYGNAGMDTLNGGEGHDYVD
                     GGPGDDTCAEGEYYVSCRH"
     gene            1406..2209
                     /gene="mutM"
                     /locus_tag="VNP73_05470"
     CDS             1406..2209
                     /gene="mutM"
                     /locus_tag="VNP73_05470"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWL65406.1"
                     /translation="MPELPEVESVRRQLDPELSGRSVTEVEYDPHPAMPRQFHRVEDL
                     IGRKIHSVGRRGKFLIAPLDQGLELVMHLGMTGAFRWDVDDDYVRARLWLDDGRMLAF
                     RDVRRFGRLAVVDAGMYESIPMLALLGPEPLSDEFDPDVFAHELARTRSPVKPFLLSQ
                     RPVAGVGNIYADEALWLARIHPMSRRVGRARALVLHGAIRDVLASAIEREGTTFRDYQ
                     MVNGESGRNASFLSAYGREGLPCPRCKNPLKKIVLGGRGTTYCPRCQRS"
     gene            2303..3373
                     /locus_tag="VNP73_05475"
     CDS             2303..3373
                     /locus_tag="VNP73_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWL65407.1"
                     /translation="MAVALATAFFGALVAGPAAAAPNDPHFARQWHFAKVQAEQAWGT
                     TTGTGVLVAVVDSGVDLTHPDLQANIVSYNDADMVEPDGNCTGPAQSRVCTQDGAQDE
                     NGHGTHVAGLIGAVANNGVGVAGVAPGAKILPVRSLNENGDGTTEQVAAGIRFAAQKG
                     ADVINLSLGIDARGHAVNLVGLLDPIHDAIAFAWAQGATIVIAAGNESTPLCAEPSAN
                     PTVVCVGALDQFDNVAWYSNTDASHQNYVVAPGGWSLGALSLGSASPTGATCQGEIFS
                     TYLRSQQSWCSAEAGYEGISGSSMSTPIVSGVAALLASRGYTNQEIVACLKTSATDIG
                     PPGRDPFYGHGRVNALAAVTTC"
     gene            complement(3374..4891)
                     /locus_tag="VNP73_05480"
     CDS             complement(3374..4891)
                     /locus_tag="VNP73_05480"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWL65408.1"
                     /translation="MDDTSRSRNAFGRSRQSADVLNQVAEPTEIAPFVWEIPMGFVDG
                     MRVPGRIFANKALFDKAMADNAAQQVANVATMPGIVTAAMAMPDIHWGYGFPIGGVAA
                     TDVDAGGVISPGGVGFDIGCGVRLIRSDLGFTKTVKPKLRELVTILGKRVPRGVGGKG
                     KLQLSGSDVDRVLREGVKFALSRGVGWEEDAELAEDFGTLSNADPDKISDRAKERGGP
                     QLGSLGGGNHFLEVQVVDEIRNRTAADAMELFEGQVCVMIHSGSRGLGHQACQEYVRD
                     IDRLMPELGIEVPDRQLGSVPMDHPAAQDYFGAMLAAGNFARANRHVLTEAAREAFMH
                     TFHKPARELGLSLVYDISHNLAKIEEYEIDGRSRRLCVHRKGATRAFGPGHPELPDRY
                     KPIGQPVIIPGSMGTASFVLVGTEEARHKSFSSTCHGAGRAMSRTKAKKEMSGQDLKR
                     ELEAQGILLAASQPKLLSEEAPYAYKDVSEVVETCEGAGISKVVARLRPVGVVKG"
     gene            complement(4951..5190)
                     /locus_tag="VNP73_05485"
     CDS             complement(4951..5190)
                     /locus_tag="VNP73_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65409.1"
                     /translation="MKMLVWGTAGKEDPTRASLPLHLVANGCIEVGHDSSIVLAGDGA
                     ELILGDTIDTLEGVGIPPVRDLFAKLKEHSIPIYV"
     gene            complement(5204..>5475)
                     /locus_tag="VNP73_05490"
     CDS             complement(5204..>5475)
                     /locus_tag="VNP73_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="archease"
                     /protein_id="HWL65410.1"
                     /translation="HSIEVTGRDLGAALVDWLSEVLYLQDSRDALIANVQVGSVTETT
                     AKGSVTVAERGEEEEVGTPVKAITYHQLKVARSGNGWETQVIVDI"
BASE COUNT          926 a         1768 c         1715 g         1066 t
ORIGIN      
        1 gttctcacgt gagtaccgac ctggtgcttc tcactatcgc ggcgggagtc atggtcggtg
       61 tcctctcggc cctcctcggg gtcggagggg gactgttgat ggtgcccttc atggtgctgc
      121 ttctcggcga gacccagcac gttgccgagg gaacgtccct ggccgtgatc gtcccaacgg
      181 cgatcgtcgg tgtgatcgca ctctcgcgcc gcgggttcgt gaacttcgtg ggcgcgggtc
      241 tgcttgccgt gggagggatc ttcggggccg gcctgggtgc ccgattggct ctgaacctcg
      301 acggcgacgt gctgaagacg gtcttcggcg ccgtcgtgat cgtgatgggt gctcgattcg
      361 tccaccaggg cctcactacg gatccaacgg ccgaacctca ttgaagcccg acgggagatc
      421 aggcgatggg cgagccggga cctacgccgt cctccaggtt gaggagtctc acggttccgt
      481 ccgggtcgag tgatccaagg atcaggaact ggctcgagaa cccggcgatc ttcttcgttc
      541 ccaggttgat cgctccgacg acgatccgac cgtccaattc ttccttcgaa tagttggtga
      601 tctgagcgct ggtggtgagc cggccgaccg tcgggccgaa atcgaccgtg agcttgtagg
      661 cgggcttgcg cgcctcgggg aagtcctcga ccgaaacgac ccggcccgtc cgcagatcga
      721 gcgcgaagaa gtccgttgct tcaatgtcct ctttcttcgg cagtttctcg ggggcgtggg
      781 gaaggttgtc ggggtcgatc tgatgctggg ccatgcccag aatctaaggg aggcaggggc
      841 tagtcttggt cgggatgagg cgaacgggat ctctaggcgt cgttgtggct cttttccttc
      901 tcgttcatcc gggcgtgccg ggtgctcaag aggagacgcg tcgatgcgac ggcgcaacgg
      961 ccacgatcgt cggcacggcc ggcaatgacc aactgacagg cacgtcgggt gatgacgtga
     1021 tcgtgggtct cggtggcaac gacgacatcc agacgagcgg cggaaccgat cgcgtgtgcg
     1081 gcaatcgagg tagcgacgtg atcgtccgaa ccgaagacac cgaccctgcc gacccggaca
     1141 tcggacgctt cttcgggggc cgcggagccg atcgggccga gggtggcaac agccccaacg
     1201 accagcaggt gttcgacacg cttcatgggg ggccgggtaa cgaccaggtc ctcggacacg
     1261 gtggcaacga tcaggtctac ggcaacgccg gaatggacac tttgaacggt ggtgagggcc
     1321 acgactacgt cgacggcggc ccgggcgacg acacctgcgc cgaaggcgag tactacgtca
     1381 gctgccgcca ctagtcggat agcgcgtgcc cgagcttccc gaagtcgaat ccgttaggcg
     1441 acagctcgac ccggaactgt cgggtcgttc cgtaaccgag gtcgaatacg acccccaccc
     1501 cgcgatgcct cgacagttcc accgcgtcga ggatctgatc ggtcgcaaga tccacagcgt
     1561 cggccgccgg gggaagttcc tgatcgctcc actcgatcaa ggattggaac tggtgatgca
     1621 tctcgggatg accggagcgt ttcgctggga tgtcgatgat gactacgtcc gagcgcgctt
     1681 gtggttggac gatggtcgga tgcttgcgtt cagggacgtt cgtcgcttcg gacgtctcgc
     1741 cgtcgtcgat gcaggcatgt acgagtcgat tccgatgctt gcgctcctgg gtccggaacc
     1801 cctttcggat gagttcgatc ccgacgtctt cgcacacgag ttggctcgaa cgagatctcc
     1861 ggtgaagcct tttctgttgt cccagcgacc ggtcgcgggg gtgggcaaca tctatgcgga
     1921 cgaggccttg tggctggcgc gcatccatcc gatgtcgcgg cgagtgggcc gcgctcgagc
     1981 cctcgtcttg cacggggcca tcagagatgt gctggcatcc gccatcgagc gcgagggcac
     2041 gaccttccgt gactatcaga tggtgaacgg cgaatccggt cgcaacgctt cgttcctgag
     2101 cgcctatggg cgcgagggcc tgccctgccc ccgctgcaaa aatccgctta agaagatcgt
     2161 tctgggcggg aggggcacga cctattgtcc ccgctgccag cgatcctgac cccggttcgc
     2221 ccccagaggc aggaatctgc cacggacatg gcaaaccttc taggtagcga aaatcccggg
     2281 aggtcacgta gtgaagagat gcgtggcggt cgcactcgcg accgcctttt ttggcgccct
     2341 cgtggcgggt ccggccgcgg ccgcgcccaa cgacccccat ttcgcgaggc aatggcattt
     2401 cgccaaggtc caggccgaac aggcgtgggg gaccacgacc gggaccggtg tgctcgttgc
     2461 ggtcgtggat tcaggtgtcg acctgacgca tcccgactta caggcaaaca tcgtctccta
     2521 caacgacgcc gacatggtcg agcccgatgg gaactgcacc gggccggctc aatctcgcgt
     2581 gtgcacccaa gacggcgcgc aggacgagaa cggacacggc acgcacgtcg ccggtttgat
     2641 cggcgcggtc gcgaacaacg gcgtgggtgt agccggtgtc gcacccggcg caaagatcct
     2701 tcccgtccgc tcgctcaacg agaacggtga cggaacgacc gagcaggttg ccgccggcat
     2761 ccgattcgcc gcccagaagg gcgcggacgt catcaacctc tcgttgggca tcgacgctcg
     2821 cggtcacgcc gtcaatctcg tgggtttgct cgatccgatc cacgacgcga tcgcgttcgc
     2881 ttgggctcaa ggtgcaacga tcgtgatcgc ggccggcaac gaatcgactc cactttgcgc
     2941 cgaaccctcc gctaatccaa cggttgtctg cgtaggagcc ctcgatcagt tcgacaatgt
     3001 cgcgtggtac tcgaacaccg atgcctctca ccagaactac gtcgtggcac cgggtggatg
     3061 gagcttgggc gctctttcgc tgggctccgc ctcgccgacg ggtgccacct gccaaggcga
     3121 gatcttctcg acctatctca ggtcgcagca atcgtggtgt tcggctgaag ccggttacga
     3181 aggtatttcc ggttcgtcga tgtccactcc gatcgtgtcc ggcgtcgccg cgctgctcgc
     3241 gtctcggggc tacacgaacc aagagatagt tgcctgcctc aagacgagcg ccaccgacat
     3301 cggccccccc gggcgagacc cgttctacgg tcatggccgc gtgaacgcgc tggccgcagt
     3361 aacgacctgc tgatcagcct ttaacgaccc ctacgggacg cagccgcgct acgaccttcg
     3421 agatccccgc gccctcgcat gtctctacga cttccgacac atctttgtag gcatagggag
     3481 cttcctcgga caacaacttt ggttgggacg cggcgagcag gatgccttgc gcttctagct
     3541 cgcgcttcag atcctgaccg ctcatctctt tcttggcctt cgtgcgcgac atcgccctcc
     3601 cggccccatg acacgtcgac gagaacgact tgtgtctcgc ctcttcggtc ccgacgagca
     3661 cgaacgaagc cgtccccatg cttcccggga tgatcaccgg ctgaccgatc ggcttgtatc
     3721 tatccggcag ctcgggatga cccggaccga acgccctcgt cgctcccttc cgatggacgc
     3781 acaggcgcct cgatcggccg tcgatctcgt actcctcgat cttggcgagg ttgtgtgaga
     3841 tgtcgtacac gagggacagc ccgagctcgc gggcgggctt gtggaacgtg tgcatgaacg
     3901 cttcccgggc cgcctcggtc agcacgtgcc ggttcgcacg ggcgaagttc cctgccgcca
     3961 gcatggctcc gaagtaatcc tgtgccgcgg ggtggtccat gggcacgctc ccgagttgcc
     4021 ggtcggggac ctctatgccc aactccggca tcagccggtc gatatcccgc acgtactcct
     4081 gacaggcttg atggccgagg ccgcgcgagc ccgaatggat catcacgcac acctggccct
     4141 cgaagagttc catcgcgtcg gctgccgtgc ggtttctgat ctcgtccacg acctgcacct
     4201 cgaggaagtg attgccgccg ccgagactcc cgagttgggg tccgcctcgc tccttcgcac
     4261 gatccgagat cttgtcgggg tcggcgttcg acaaggtgcc gaagtcctcc gcgagctcgg
     4321 cgtcctcctc ccagccgacg ccgcgcgata gcgcgaactt cacaccctct cggagaacgc
     4381 gatcgacgtc ggacccggaa agttggagct tgcccttgcc tccaacgccg cgcggcaccc
     4441 tcttgcccag aatggtgacg agctcgcgaa gcttcggctt caccgtcttg gtgaatccga
     4501 gatcactgcg gatcagtcga accccacacc cgatgtcgaa gccgacgccg ccgggcgaga
     4561 tgaccccgcc ggcgtcgaca tcggtcgcgg ccacgccgcc gatcgggaac ccgtaacccc
     4621 aatggatgtc gggcatcgcc atcgctgccg tcacgatgcc cggcatcgtg gcaacgttgg
     4681 ctacctgctg agcggcgttg tccgccatcg ctttgtcgaa cagagccttg ttggcaaaga
     4741 tccggcccgg gacgcgcatg ccgtccacga atcccatcgg gatctcccac acgaacggag
     4801 ctatctccgt gggttccgcg acctggttca gaacgtcagc actttgtcgg cttcggccga
     4861 aagcgttgcg gcttcgggag gtgtcgtcca ccggccattg acccttgcga gatcttcttc
     4921 ggtcgcccca cgggcgactg cgcacgcttt tcagacgtag atggggatcg aatgctcctt
     4981 gagtttcgca aagagatctc gcaccggagg gatgccgacg ccttccagag tgtcgatggt
     5041 gtctccgagg atgagttctg cgccgtcgcc ggccaggacg atcgacgaat cgtgacctac
     5101 ttcaatgcaa ccgttggcta cgaggtgtag cggtagggac gcacgggtcg ggtcttcctt
     5161 gcccgccgtt ccccacacca acatcttcat ggccgctcct ctcctagatg tcgacgatca
     5221 cttgcgtttc ccatccgttg cccgaacgcg ccaccttcaa ctggtgatag gtgatcgctt
     5281 tgacgggcgt tcctacttct tcctcctccc cacgttcggc cacagtgaca cttcccttcg
     5341 ccgttgtctc ggtcaccgag cccacctgta cgttcgcgat caacgcgtct ctggagtctt
     5401 gcaggtagag gacctcactc agccagtcga cgagggccgc gcccaggtcg cgcccggtga
     5461 cctcgatcga gtgaa
//
LOCUS       DATMED010000217         6036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4385848, whole genome shotgun sequence.
ACCESSION   DATMED010000217 DATMED010000000
VERSION     DATMED010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385848"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VNP73_05495"
     CDS             complement(<1..883)
                     /locus_tag="VNP73_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65411.1"
                     /translation="MATRLFDLNIEEVLEAWEVSHAIREVISNALDEHQLSGTKDISI
                     ESVGPDHWSIRDFGRGLQIEHFTLNENQEKLAADSGVIGKFGVGLKDALATFHRRGVD
                     VIIRSRHGSFSLQETEKHSFSGITTLHVAYDSTPNDMDGTEFELSGVTTEQMDEAKSL
                     FLAFNDEKVLDATRYGEVLRRRKDGGRVYISGVFASEEPNFLFSYNVTNLTDAMKKRL
                     NRERMNVGRTTYADRIKQILKEAEADEVIEELIDEILGRGRKQVRDEMQWIEISQLSL
                     TRLYERDKVIFVTEDELH"
     gene            1020..2174
                     /locus_tag="VNP73_05500"
     CDS             1020..2174
                     /locus_tag="VNP73_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65412.1"
                     /translation="MRKVAALLAAALLVGSLTAQTASAVTAGPSPGGMSSDNVEWLSH
                     IPLSVDGVGGRVVDGYFYTNDQHKVMIFDLKDPVNPQLVGQVLMPQEWLYSREDIDTN
                     GKIMVVPNTVTASADLQPSAGTNAAYIIDVEDKANPRIISKVVGGAQHTLSCILDCKW
                     VYGSDGIIIDVRNPLKPKLVDKEWGEGLPAGGGHDVEEIAPGYVLTATQPIQLLDVHN
                     PLKPKLLAMGRNTDGRFIHTGRWVDLNDKFLLMAGETNNRVRCNETAAAFMTWDASTW
                     RKTHTFNMIDEYRMVNGTYADGNPAVNQVGCSSHWHEAHPDFHNGGIVAAAFFEHGTR
                     FINVTGTGKIQEKGWFMPAAGSTGAVYWVTDEIVYAVDYNRGIDILRWKG"
     gene            2185..2859
                     /locus_tag="VNP73_05505"
     CDS             2185..2859
                     /locus_tag="VNP73_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65413.1"
                     /translation="MRKLLAIALTGGLLVGMLVPAQAKPAQVFEDIAGDAGNQDSGVP
                     GFDQLGFDLIGGTIERKGANLDFTVLHAAMPPVNQPPEKVRFMWGFAVDGTSYRVTVK
                     SADVGKPNPVAQSDTDRIGKVDPQGFFRLEGDCGSQVVGALQAVHCPTIGYLTGAFDA
                     AAKTISFSVPLKLIKAKPGSVISGGGGDAVSICAICWTTHVGERSLNTTNIDSAAQSG
                     TYKIPK"
     gene            2872..3555
                     /locus_tag="VNP73_05510"
     CDS             2872..3555
                     /locus_tag="VNP73_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65414.1"
                     /translation="MRKATALILAGGMLFGMLVPAQAKPVEVFTDIAGDAGNQDSGVP
                     GFTEGGLDLAGGSIERKGANLEFTVAHHGAMPNFGHMPEVARFMWHFSVDTKNQFRFS
                     VKSVDIGKPDAIAGTGTERVGQVYATGTARLETCEETPSPAITLINCSAIEYLDVKID
                     AAAGTFTVIAPMKLLKAKPGSVVSGSAHASGGSGCMICWVFHLAERSLTATTIIDSAG
                     QAVSYKVPK"
     gene            3566..4240
                     /locus_tag="VNP73_05515"
     CDS             3566..4240
                     /locus_tag="VNP73_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65415.1"
                     /translation="MRKALALILAGGLLVGMLSPAHAKPVSVFTDVTGDAGNQDSGVP
                     GADQAGFDLVGGTIEQKGANLEWTVEHAAMPASGQPGEGFRFLWHFTVDTKGQYRWTF
                     KTVDLGKPDAIAGTGTERVGQVYQNVVRLEECSETPSPAVTLVNCSAIAYYDAVVDPA
                     AKTFTIVSPLKDLKAKKGSVIAGGTHAAGGTSCMVCWVPHYAERSLTPQSVIDSAGIA
                     KEFKVG"
     gene            4316..5353
                     /locus_tag="VNP73_05520"
     CDS             4316..5353
                     /locus_tag="VNP73_05520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWL65416.1"
                     /translation="MALKIGVGLFTGQIPVDSPRTFHQEYQDYLELVRRIEDVGLDSA
                     WVSEHHFAADGYLPSLALMLAAFAAVTDRIELGTGVVLAPFHDPIRLAEDFAVVDQLS
                     GGRVISGFGIGWRDEEFREFNIETKSRVRRLEEIVEILRLAWNEERFSYNGKYYTYSD
                     VSVSPKPARVPPIFIGGFVDDAIRRGGRIGDGYLSSRAQPERVAQAFEMAAEERAKAG
                     LDGPPLVGLLHNAFVTDDPDRDWPMVRDGIGHQLGVYTGWREGTDLPGKPLEVKPPDE
                     EIIRKTTAYGTPDQVVESLQPVVEVLTRYPESHLILRLHYPGMEAEPAARAIELLGTK
                     VAPQLKQRSPQ"
     gene            5422..>6036
                     /locus_tag="VNP73_05525"
     CDS             5422..>6036
                     /locus_tag="VNP73_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65417.1"
                     /translation="MLALGGIYLWVSMLTGPWMLAGGLLDAADHFGTAQKKLTAGALK
                     DARYETLAGVAAADRARRGLDSNSPLFDLASMHPRIRGTLGEADHFVAAAQLSGRAAN
                     GTLDIAQNALRGPNKIIVKDPDDPKGGARIRIDRVREIARTVGHIRGAIGGVDEQLKK
                     VNLAKIPRRLRPQVKRGIEQASTTRELLTKAEAGLEILPSFLGAD"
BASE COUNT         1168 a         1908 c         1883 g         1077 t
ORIGIN      
        1 tgtgtagctc gtcctcggtc acaaagatga ccttgtcccg ttcgtacagc cttgtaaggg
       61 acaactggct gatctcgatc cactgcatct cgtcccggac ctgcttgcgg ccgcgcccga
      121 ggatctcgtc gatcaactcc tcgatgacct catcggcttc cgcttccttc aggatctgct
      181 tgatgcgatc cgcatacgtc gtccgcccga cgttcatgcg ctctcgattg agccgcttct
      241 tcatcgcatc cgtcaggttc gtgacgttgt aagagaagag gaagttgggt tcctcgctgg
      301 cgaaaacacc gctgatgtaa acgcggcccc cgtccttgcg cctgcgcaac acttcgccat
      361 atcgggtcgc gtcgagcacc ttctcgtcgt tgaatgccag gaacagcgac ttggcctcgt
      421 ccatctgctc ggtagtcacc ccagacagct cgaactccgt cccgtccatg tcgttcggtg
      481 tcgagtcgta ggccacgtgc agtgtcgtga tccccgagaa cgaatgcttc tctgtctcct
      541 gtagcgagaa gctcccgtgc cgagatcgga tgatcacgtc cacaccgcgc cgatggaacg
      601 ttgcaagagc gtccttcaaa cccaccccga actttccgat cacgcccgag tcggcggcaa
      661 gcttctcctg gttctcgttg agcgtgaagt gctcgatctg cagcccccga ccgaaatcac
      721 ggatcgacca gtgatccggg cccacggact cgatcgagat gtccttggta cccgaaagct
      781 gatgttcgtc gagagcgttg gagatgactt cacggatcgc gtggctgacc tcccacgcct
      841 ccaggacctc ctcgatattg agatcgaaaa gtcgagtcgc catgtgtgcg ccagcctagg
      901 tccgccgatc tggacatatg ggacagctcg ggctgtctgc aggaatgggg tcgcatgggc
      961 cgaatccctg atgacagacg aaaaaacccc gtgtggggct attcccaggg agggatttga
     1021 tgcgcaaggt tgcagctctt ctggcagcag cgctgctggt cggaagcctg acggcccaga
     1081 cggcctcggc cgtgacggca gggccttcgc ccggcggcat gtcctcggac aacgtcgagt
     1141 ggctctcaca catcccgctc tcggtagacg gggtcggggg ccgcgtggtc gacgggtact
     1201 tctacacgaa cgaccagcac aaggtcatga tcttcgatct caaggacccg gttaacccgc
     1261 aactggtcgg acaggtcctg atgccgcagg agtggctcta ctcccgcgaa gacatcgaca
     1321 cgaacggcaa gatcatggtc gtcccgaaca ccgtgacggc ctccgccgac ctacagccgt
     1381 cggccggaac gaacgccgcc tacatcatcg acgtagagga caaggcgaac ccacggatca
     1441 tctccaaggt cgtcgggggc gcgcagcaca ccctgtcctg catcctcgac tgcaagtggg
     1501 tctacggctc cgacgggatc atcatcgacg tccgcaaccc cctcaagccc aagctggtcg
     1561 acaaggaatg gggcgaaggc ctcccggccg gaggtggaca cgacgtcgag gagatcgctc
     1621 ccggctacgt gctcacggcc actcagccga tccaattgct cgacgttcac aaccctctca
     1681 agccgaagct tctcgcgatg ggtcgcaaca ccgacggccg tttcatccac acaggccgct
     1741 gggtggacct caacgacaag ttcctgctta tggccggcga gacgaacaac cgagtgcggt
     1801 gcaacgaaac cgccgccgca ttcatgacgt gggatgcctc gacgtggcgc aagacgcaca
     1861 cgttcaacat gatcgacgag taccgcatgg ttaacggcac gtacgccgat ggcaacccgg
     1921 ccgtcaacca ggtcggttgc tcctcgcact ggcacgaagc acaccccgat ttccacaacg
     1981 ggggcatcgt ggcggccgcg ttcttcgagc acggcactcg cttcatcaac gtgaccggca
     2041 ccggaaagat ccaggagaag ggctggttca tgccggccgc gggatccacc ggcgccgtct
     2101 attgggtcac cgacgagatc gtttacgccg tcgactacaa ccgtgggatc gacatcctgc
     2161 ggtggaaggg ctagggggga cgagatgcgc aagctactcg ctatcgcact aacggggggg
     2221 ctcctcgttg ggatgctcgt tcccgcgcaa gcaaaacctg cccaggtatt cgaagacatt
     2281 gccggcgatg ccggcaacca ggattccggc gttcccggtt tcgatcagct cggcttcgac
     2341 ctcatcgggg gaacgatcga gcgtaagggc gcgaacctcg acttcaccgt ccttcacgcg
     2401 gcgatgcctc cggtgaacca gccccccgag aaggtccgct tcatgtgggg cttcgcggtg
     2461 gacggcacgt cttatcgagt cacggtcaag agcgcggatg tcggcaagcc gaatccggtc
     2521 gctcagagcg acacggatcg gatcggcaag gtcgaccctc agggattctt ccgtctagaa
     2581 ggagactgcg gaagtcaggt agtgggagca ctgcaagcgg tgcactgccc gacgatcggt
     2641 tacttgacgg gagcgttcga cgctgcggcc aagacgatct cgttctccgt gcccttgaag
     2701 ttgatcaaag cgaagcccgg atcggtcatc tccggcggag gcggcgacgc ggtctctatc
     2761 tgcgccattt gctggacgac ccacgtcggc gagcgctcac tcaacaccac gaacatcgat
     2821 tctgctgctc aatccggcac ttacaagatc cccaagtagg aggaaaagac gatgcgcaaa
     2881 gcaacagctc tcatcctggc gggaggcatg ttgttcggca tgctcgtacc cgcgcaagca
     2941 aagcccgttg aggtcttcac cgacatcgcc ggcgacgcag gtaaccaaga ctccggcgtg
     3001 cccggcttca ccgagggtgg gctggacctc gctggtggtt cgatcgagcg caagggcgcg
     3061 aacctcgagt tcacagtggc gcaccacggt gcgatgccga acttcggcca catgcctgag
     3121 gtcgccaggt tcatgtggca cttcagcgtc gacacgaaga atcagttccg cttcagcgtg
     3181 aagagcgtcg acatcggcaa gcccgatgcc atcgccggga ccgggacgga acgggtcggc
     3241 caggtctacg caaccgggac cgctcgcctc gaaacttgcg aggagactcc gtctccggcg
     3301 atcaccttga tcaactgcag cgcgatcgaa tacctggacg tgaagatcga tgccgccgca
     3361 gggacgttca cggtgatcgc gccgatgaag cttctcaagg cgaagcccgg ttccgtggtc
     3421 tctgggagcg cacacgcttc gggtggctcg ggctgcatga tctgctgggt attccatctc
     3481 gccgagcgtt cgttgactgc caccacgatc atcgactcgg ccggtcaggc cgtctcctac
     3541 aaagttccca agtaggagga tgacgatgcg taaagcacta gctctgatac ttgcgggagg
     3601 cctgctcgta ggcatgctgt ctccggccca tgcgaagccc gtctccgtct tcactgacgt
     3661 caccggtgac gcagggaacc aagactccgg ggtgcccgga gccgaccaag caggattcga
     3721 cctcgtcggc ggaacgatcg agcagaaggg cgcgaacctc gagtggaccg tcgagcacgc
     3781 cgcgatgcct gcgagcggtc agcccggcga agggttccgg ttcttgtggc acttcaccgt
     3841 cgacacgaag ggtcagtacc ggtggacgtt caagaccgtc gacctcggca agcccgacgc
     3901 gatcgcaggc acggggaccg aacgggtcgg ccaggtgtac cagaacgtcg tgcggctcga
     3961 ggaatgctcc gagaccccga gcccggccgt aacgctcgtc aactgcagcg cgatcgctta
     4021 ctacgatgcg gtggtcgacc ccgcggccaa gacgttcacg atcgtctcgc cgttgaagga
     4081 cctcaaagcg aagaagggtt cagtgatcgc cggcggcacg cacgccgcgg gtggaaccag
     4141 ctgcatggtg tgctgggtcc ctcattacgc agagcgctcg ctgacgccgc aatccgtgat
     4201 cgactccgcg gggatcgcga aggagttcaa ggtcggttag acacttctgc ttaactgtta
     4261 gcgtgaccgg ggccttcggg ccccggtttt ctttgtgtgg cgaggaggtg gcggcatggc
     4321 tttgaagatc ggtgtcgggc tgttcaccgg tcagatcccc gtcgatagtc cccggacgtt
     4381 ccaccaggag taccaggact acctcgagct cgtccgccgg atcgaagacg tcgggctgga
     4441 ctccgcgtgg gtctcggagc atcacttcgc tgctgacggc tatctgccct cgttggcgct
     4501 catgttggcc gccttcgcgg cggtgaccga ccggatcgaa ctgggaaccg gtgtcgtact
     4561 ggcccccttc cacgatccga tcaggctggc cgaggacttc gccgtcgtcg atcaactgtc
     4621 cgggggccgc gtgatctcgg gattcgggat cggctggcgc gacgaggagt tccgcgagtt
     4681 caatatcgag accaagtcac gggtccgccg gctggaggag atcgtcgaga tcctccgact
     4741 cgcctggaac gaggagcgat tcagctacaa cggcaagtac tacacctact cggacgtgtc
     4801 cgtttcgcct aagccggctc gcgtcccgcc gatcttcatc ggtggcttcg tcgacgacgc
     4861 gatcagacgg ggcggccgca tcggcgatgg atatctgtcg tcgcgcgcgc aacccgagcg
     4921 tgtcgctcaa gcgttcgaga tggcggccga agaacgagcg aaagccggcc tcgatgggcc
     4981 gcccctcgtc ggcctcctcc acaacgcgtt cgtgaccgac gacccagacc gggactggcc
     5041 gatggtgcgt gatggcatcg gccatcagct gggtgtgtac accggctggc gcgagggcac
     5101 cgacctaccc ggcaagccgc tggaagtgaa accaccggat gaggagatca tccggaagac
     5161 gacggcgtac gggacaccgg atcaggttgt ggaatcccta cagccggtcg tcgaagtgct
     5221 gactcgctat cccgagagcc acctgatcct ccgcttgcac taccccggga tggaagccga
     5281 acctgcggcg cgggcgatcg agctactcgg aacgaaagtg gcgccacaac tgaagcaacg
     5341 gagcccgcag taacggatcc cgggcgcgac gccggtccag atcaccgctc cggagcaggg
     5401 ctcgcgggag ttgcggcggt cgtgctcgct ctgggcggga tctacctatg ggtctcgatg
     5461 ttgaccggcc cgtggatgct cgcgggaggt cttctcgatg ctgccgacca tttcggaacc
     5521 gctcagaaga agctgacggc cggggcgctc aaggacgctc gttacgaaac acttgcaggc
     5581 gtagccgccg cagatcgagc gcggcgcggc ttggactcga acagccccct attcgatctg
     5641 gcttcgatgc acccgcggat ccgcgggacc ttgggtgagg ccgatcactt cgtagccgcg
     5701 gctcaactgt ccggtcgagc cgcgaacggg acgttggaca tcgctcagaa cgcactacgc
     5761 ggaccgaaca agatcatcgt caaggatccc gacgacccga aaggaggcgc gcggatccgc
     5821 atcgatcggg tacgtgagat cgctcggacc gtgggccaca tccgaggcgc gatcggaggg
     5881 gtggacgagc agctcaagaa ggtgaacctc gccaagatcc ctcgtcgcct ccgcccccag
     5941 gtcaagcgag ggatcgagca ggcatccacg acgagggagt tactcacgaa ggccgaagca
     6001 gggctcgaga tcctgccgag ctttctcggg gccgac
//
LOCUS       DATMED010000218         3359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2485311, whole genome shotgun sequence.
ACCESSION   DATMED010000218 DATMED010000000
VERSION     DATMED010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485311"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VNP73_05530"
     CDS             complement(<1..663)
                     /locus_tag="VNP73_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65418.1"
                     /translation="MRRVLSLCFLATLLGANAVQSARAQDCSWKQVDVGALISGAGLV
                     DVRALAPDDVWVIGRRSGESKTLAIHWDGSTWTSTRTPNRHESTVRGVYALDASGPRN
                     VWAVGFTQQLPEGDYPGSIGPPEPLIEHYNGEKWSVVTTGLPPDDHGPVLHDVLVHDK
                     DDVWIVGTRWFDSGRGKALVMHFNGSKWKIVHSGNKPKQERSLHVVTSTQPNTIWAVG
                     NRA"
     gene            complement(707..991)
                     /locus_tag="VNP73_05535"
     CDS             complement(707..991)
                     /locus_tag="VNP73_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65419.1"
                     /translation="MTARRPHLELVSIDGEPTPIERDAIQKALEEFVIAERREREGSL
                     WMRAGRSKGRRLGMSDYRDRFSHEDAWRLSARFPYGGREYPGLVGRGDAK"
     gene            complement(988..2577)
                     /locus_tag="VNP73_05540"
     CDS             complement(988..2577)
                     /locus_tag="VNP73_05540"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWL65420.1"
                     /translation="MQQPQDPSPADAGHPIQEQIQGLRDRRDQAHLAGGEAAIQKQHD
                     RGKLTARERVEKLLDVGSFVETDQLVRHRSHGFGIENRRPYSDAVVTGWGTIEGRKVF
                     VFAQDFTVFGGSLGEVMGEKICKIMDLALETGAPVIGLNDSGGARIQEGAASLAGYGY
                     IFDRNVRSSGVIPQISAIMGPCAGGAVYSPAMTDFIFMVSETSHMFITGPDVIKTVTG
                     EEVSQEELGGAMSHASKSGVAHFVAANDEDCLQQIRYLMSFLPSNNFESPPYFPPTDD
                     PNRSCDELLDLIPDSSNKPYDMHQVIASVFDDGEFYEVHSQWGQNILCGLSRLNGHPV
                     GVVANQPQVLAGCLDITSSTKGGRFVRFCDAFNIPLISFVDVPGFLPGTDQEYGGIIR
                     HGSKLLYAFSEATVPRMTVITRKAYGGAYVVMNSKHIRADISFAWPSSEIAVMGAEGA
                     VNIIHRKEIVSAEDADARRAQLVSEYNELFLTAYIAAERGYVDAVIEPQDTRRKLVQA
                     LEMLRSKRETIPQRKHGNIPL"
     gene            2694..>3359
                     /locus_tag="VNP73_05545"
     CDS             2694..>3359
                     /locus_tag="VNP73_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65421.1"
                     /translation="MSTVRLWRDAKDLYRSFVDLLIFDDPGEEAGYELALSARRFSEN
                     TQAVVDDKLNFSATLMRAGEVDAANRLLAEFERDVREEEAALIERVNEVKVAEAVRRE
                     GLTRVRLARVLAVALVGSSLLTFSAAGMALAGFVRDRVLFDEGDGNRVRVPETVEGAV
                     AVAQADNEVLKPKLKHIRIGNVELMLTASQLRTLRDLAGGTPNESGLHDIIGLLPEQL
                     AEKV"
BASE COUNT          622 a         1025 c         1042 g          670 t
ORIGIN      
        1 cgcacggttg ccgaccgccc agattgtgtt gggctgagtg ctcgtgacca cgtggagtga
       61 gcgctcctgt ttgggcttgt tgccactgtg cacgatcttc cacttactcc cgttgaaatg
      121 cataaccaaa gccttgccgc gcccgctgtc gaaccaacgt gtgcccacga tccagacgtc
      181 gtctttgtcg tggacaagga cgtcatgcaa aacaggaccg tggtcgtcag gtggtaggcc
      241 ggtggtaacg acactccact tctcaccgtt gtaatgctcg atcaacggct cgggaggacc
      301 gatcgagccg ggataatcac cctcgggtag ttgctgggtg aaaccaacgg cccacacgtt
      361 cctcggaccc gaagcatcca aggcgtacac gccgcgaaca gttgattcat gacggttggg
      421 tgtgcgggtg ctcgtccagg tgcttccgtc ccagtggatt gccaaggttt tggattcgcc
      481 cgatcgtcgg ccgatcaccc acacgtcgtc aggtgctagt gccctaacgt cgaccaggcc
      541 ggcgccggag atcagggccc ccacatccac ctgcttccaa ctgcagtcct gagccctggc
      601 actttgaacc gcgttcgcgc cgaggagggt tgctaagaaa cagagggaaa gaactcgcct
      661 catgacgctc ttaacggcag ccgttccgcg ggagtggctt gcgttgctat ttggcgtcgc
      721 ctcggcctac cagccccgga tactcgcggc ccccatacgg gaaccgagcc gacaaacgcc
      781 atgcgtcctc gtggctgaac cggtcgcggt agtccgacat cccgagccgc cgtcctttgg
      841 atcgacccgc tcgcatccac aatgaccctt cgcgctctct gcgttcggcg atgacgaact
      901 cttcgagcgc cttctggatc gcatcgcgct cgataggtgt tggttctcca tcgatcgaca
      961 cgagttccag gtgcggtcgc cgggctgtca tagggggatg ttgccgtgct tgcgctgagg
     1021 gatcgtctcg cgtttggagc gcagcatctc gagagcttgc acgagtttgc gacgcgtgtc
     1081 ctgtggctcg atgaccgcgt ctacgtagcc gcgttcggcg gcgatgtaag ccgtgaggaa
     1141 gagttcgttg tattcggaca cgagttgtgc acgtcgagcg tcggcgtcct cggctgagac
     1201 gatctccttg cgatggatga tgttgacggc cccctctgcg cccatcacgg cgatctccga
     1261 tgaaggccac gcgaacgaga tgtcggcacg gatgtgcttg gagttcatga cgacgtacgc
     1321 gccgccgtag gccttccgag tgatcaccgt catcctcgga accgtggctt cgctgaacgc
     1381 gtacagcaac ttcgatccgt gacggatgat ccctccgtac tcctgatccg ttcccggaag
     1441 gaagccgggg acgtccacga acgagatcag tgggatgttg aacgcgtcgc agaagcgcac
     1501 gaagcgcccg cccttggtcg aggacgtgat gtcgaggcaa ccggcaagca cttgcggctg
     1561 gttggcaacg acgcccaccg ggtgaccgtt caagcgagag agcccgcaca ggatgttctg
     1621 cccccattgc gagtgcactt cgtagaactc gccgtcgtcg aagacggagg cgatcacctg
     1681 atgcatgtcg taaggcttgt tcgatgaatc ggggatcaga tccagcagct cgtcgcacga
     1741 cctattcgga tcgtcggtgg gcgggaagta gggaggagac tcgaaattgt tcgaaggaag
     1801 gaacgacatc agatatctga tctgttgcag gcaatcctca tcgttcgcag ccacgaagtg
     1861 agcgacccca gatttcgagg catgactcat cgcgccgccg agctcttcct gacttacctc
     1921 ttcgcccgta accgtcttga tgacgtcggg tccggtgatg aacatgtgtg aggtctccga
     1981 caccatgaag atgaagtcgg tcatcgccgg ggagtaaacg gctccgcccg cacacggccc
     2041 catgatcgcc gagatctggg ggatgactcc ggaagaacga acgttgcgat cgaagatgta
     2101 gccgtaaccg gccagcgagg cggctccctc ctggatgcgc gctccgcccg agtcgttcag
     2161 cccgataacc ggcgccccgg tttcgagggc caggtccatg atcttgcaga tcttctcgcc
     2221 catgacctcg cccagtgacc ctccgaagac cgtgaagtcc tgagcgaaga cgaagacttt
     2281 ccgaccttcg atcgttcccc aaccggtgac aaccgcatcg gaatagggcc ttcggttctc
     2341 gatcccgaag ccgtggctcc ggtgccgcac gagctgatcc gtctcgacga acgaaccgac
     2401 gtcgaggagt ttctcgacac gctcccgggc agtgagcttc cctctgtcgt gttgtttctg
     2461 gatcgcggct tcgcccccgg ccagatgggc ctgatcgcgg cgatcacgga ggccctggat
     2521 ctgttcctgg atcgggtgcc cggcgtctgc tggagatggg tcttgaggtt gctgcatagt
     2581 gggcggcagt ttagtcccac cccgtccccg gccccttgcg aaatacctgc aaatcgggca
     2641 aaaagccgcc aggtccggca ggggagtgcc acctggaggc gaaccccaca ggtatgagca
     2701 ccgtgaggtt gtggcgcgat gcaaaagacc tttaccggag tttcgtcgat cttttgatct
     2761 tcgacgatcc gggcgaagag gccggctacg agctggccct ctctgcgcgc cgcttctcgg
     2821 agaacaccca agcggttgtc gacgacaagc tgaacttctc ggccactctg atgcgcgccg
     2881 gcgaggtcga cgcggccaat cgtcttctgg ccgagttcga gcgcgacgtg cgcgaagaag
     2941 aggcggcgct catcgagcgc gtcaacgagg tcaaggttgc cgaggccgtc cggcgtgagg
     3001 gtctcactcg tgtccggctg gcccgcgtgc tggccgtcgc cctcgtgggt tcgagcctcc
     3061 taactttctc ggccgcgggc atggcccttg ccggattcgt cagggacaga gtcttgttcg
     3121 acgagggtga tggcaacagg gttcgggtgc ccgagacggt cgagggcgcc gttgccgtgg
     3181 cgcaggccga caatgaggta ctgaagccca agctcaagca catcaggatc ggcaatgtcg
     3241 aactcatgct gacggcttcc cagttacgca cgctcagaga tctcgccggg ggaacaccta
     3301 acgaatcggg tctgcacgac atcatcggac tcctcccgga gcagttggct gagaaggtt
//
LOCUS       DATMED010000219         9183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2486178, whole genome shotgun sequence.
ACCESSION   DATMED010000219 DATMED010000000
VERSION     DATMED010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486178"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VNP73_05550"
     CDS             <1..1441
                     /locus_tag="VNP73_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL65422.1"
                     /translation="DPRGVSQIDDPLLMSWVDLVALGRQHRESDPGALEDLLEAEPDD
                     VMTLIYTSGTTGPPKGAMLTIRNVEFAIQELVEGGGFTSPPPTNSDVLLSYLPLCHVA
                     ERIFTVWFNLSAGCVVNFAESIETVTQNLQEVQPTIFLGVPRIWEKMHAGIQIRAANA
                     SRLKRWNFRLWMKVADRIADRLVDTGGVHTLGTRMLYSIGYVFLFRALKERIGMLRCR
                     YAASGAAPIAPEILRFFMGIGVPMHEVYGMTENSAVATGNRPGRIKLGTVGEPHANVE
                     LKIDEHTGEILTRHPGIFVGYWNDPEATADAVTADGWLHTGDVGEWVDGTHVKITDRM
                     KDIIITSGGKNVSPSEIENSLKTSPFIKEAIVVGDGRKYLTALVGIEMDTVGNWAQRR
                     GITYTTYRNLSENPEVEKLVRGIIDDVNRGFSSVEQIKAFRMLTKELDHEDGELTATQ
                     KVKRAAIAGMFGDLVEEMYGGKPTAARST"
     gene            1438..2331
                     /locus_tag="VNP73_05555"
     CDS             1438..2331
                     /locus_tag="VNP73_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL65423.1"
                     /translation="MTRFIQALVIGLGLASIYSLLALGFVIIYKSMRVISFAQPGLMI
                     LGAYFVIYFGTVVDLSFWIALPLAMATAAGAGLLIERLAIRPMVGKPVFAVAMITIGI
                     DIALRVLVNDLLGSNVRAVGDPWGLGTVEALGVSIQHRRIAMVIFAVVVVSLLFAFFK
                     YTRIGLAMRASSFDQEAALAQGISVGSVFALSWAFAGALAALAGMFVGTGGGIDQGSA
                     FVALKALPAVILGGLDSIWGAVVGAIIIGIFEGLSVTYQGQYLSFLGGNFSQVVPYLV
                     MLVVLLVRPYGLFGTPEIERV"
     gene            2328..3440
                     /locus_tag="VNP73_05560"
     CDS             2328..3440
                     /locus_tag="VNP73_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL65424.1"
                     /translation="MRRRPRMYTAYEQDSALLNTSAKKIWMASLLVCLLVLPFLLNDA
                     LVVLVATAFVASIGAIGLNIVTGYAGQVSLGHAFFFGLGAYTAAVLGGDPEGQLVGYG
                     VDMEVWLPAAGIVAALAGLLVSPVAFRLRGLYLAIVTLGLVFIGEHIFREAESLTGGT
                     GVGRTAARAVLFGFAWTDQGQIAGIELSRSQGLYFMSLIILVILALPARNLARSAIGR
                     AFAAVRDRDLAAEAMGISLAKQKALAFTISSFYAGIAGALLATITGYLEPGSFNLLLS
                     VRYIAMILIGGVATISGSIMGAAFVTLLPRAIQEIPRFVPIISEAATGGVLTTQQLEQ
                     IIYGLLIIFFLILEPRGLYGLWVRIRNYWTQWPFSY"
     gene            3510..4838
                     /locus_tag="VNP73_05565"
     CDS             3510..4838
                     /locus_tag="VNP73_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL65425.1"
                     /translation="MKGKLGWVRTIALLMMLGVIASACASGGDVAEEDDGGGDAEIAT
                     DVGVTEEPCPKAVNEDNGCIFLGTLSDLTIGPFAPLAVPITDSQAAFWNRVNEEGGIG
                     DEYDVDVTTHVQDNEYNPEVHVRKYQQIEPDVLALAQTLGTPPTLAALDLYKQGDVVG
                     APASWWSGWEFEDVIIESGTNYCLESMNAIDYAIEEFNSKTVMAVGYPGDYGGDAAAG
                     AEIAAKENGLEFVGAIETAPNAVAGSQEEAIGAVLEANPDLVIVTTGPTEMAEIVGGT
                     AARDFKGRFMGTSPTWNKGVLDSPAAPAIEALYLQTAPWAPFDTDTPGHQAMREAVGD
                     VDPNDGYTSGWVWSYPLRQVLEDAFASGDLTRAGLVEAASALETVDYEGMLPEEAGRF
                     AGDPNDTVFRQIVINKPNKKAPSGVTLLEDFFVGPTAEAFEFTEPCQAAE"
     gene            complement(4897..6450)
                     /locus_tag="VNP73_05570"
     CDS             complement(4897..6450)
                     /locus_tag="VNP73_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWL65426.1"
                     /translation="MEPRSGRRLLASLIAVACLALAAGTTSVAASAAPTKECPPSVAG
                     LDLQTVTIPELQEALASRKTTSRDLVWAYLARIDAYDEHTNSIRALNPKALKIAGKLD
                     AERRSGETRGPLHGIPVLLKDNINTLDMPTTAGSIALKGSIPLEAATITTKLEEAGAI
                     ILGKTNLSEFANWVDLSMPNGYSSLGGQVRNAYTNGDPSGSSSGSGVAGSMAFATGTV
                     GTETSGSILSPSMANSLVGIKPTTGLLSRFGILPLAPSFDTPGPMTRTVTDAAIMLGA
                     MAGVDDKDPRTADSGDAPSDYSKFLDDNALKGVRLGYSPQDVGSNGLFEKALTVLEEQ
                     GATLVETDNLSNTSLAGLTEIGLIPNEFKASLNQYLAEQTPDSLRVRTLSDIVAYNEE
                     HPDKVKYGQNLLIASDAQPGVYHPATAEPTIQSSRAVIDATLAADNLDAIVAPGNTYA
                     NVSAAAGYPTVIVPAGYAGQNPFGLSFLADGFSEPRLISFAYDYEHATKLRIPPTDLN
                     EVLIPKRCG"
     gene            complement(6492..6998)
                     /locus_tag="VNP73_05575"
     CDS             complement(6492..6998)
                     /locus_tag="VNP73_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HWL65427.1"
                     /translation="MNVSDLLVDAFGRVHETVHGVVKDLRPEQLEFRASVDANTIAWL
                     VWHLTRVQDDHVADVAGTEQVWTSAGWSDRFALPLDTSDTGYGHSSDQVAAVRVSSDL
                     LTGYHNAVHDQTLDYLRGLSESDLDRIVDERWDPPVTLGVRLVSVIEDDLQHVGQAAF
                     IRGILPKS"
     gene            7106..7282
                     /locus_tag="VNP73_05580"
     CDS             7106..7282
                     /locus_tag="VNP73_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65428.1"
                     /translation="MKKIRVLLVACLAAAFMAVGAGPASAKCVGEPNVCVLVCEVGTS
                     NKYTEELFEFCYVW"
     gene            complement(7694..>9183)
                     /locus_tag="VNP73_05585"
     CDS             complement(7694..>9183)
                     /locus_tag="VNP73_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL65429.1"
                     /translation="PQQPWSNGKVGLLGHSYSGATAMLIASHQPQHLAAMTVSGLVDD
                     NYRGITFPGGVLNTLFPPLWYLGIRNGYHVVGGSGQGIARNLDNENGQRCAANTATHT
                     VDMTNDPILNGVMSQGLDSDYWHRVSMITYIDKINVPIHITGTFNDEQTGARGTAHLW
                     EEVRPGLPKRLLQSNGNHDTNVVAEETWADRKAWMDYWMRGVTPDPRWDMATPSGKIT
                     RASVRTLFELHPDEDGALVSNGHYDSTRWPLDGTNWTTAYLCAGKALEFDRTQCGEGS
                     DSYLSGTRRQSWLFQAGPDLGPPITSEDGPDQVFLQGPVVGPDETWAIAGPIIADLRM
                     SITGNNTDLFVQVADLDTESKEIKFLTRGWLKASHHAIDEKLSDYSGGFMYRPFRHHT
                     EPENVAPDEPVDYKIEVWPTAHVFRPGHQLVVIVTAPPAIDSNYSFAVQSNQPASINT
                     VIYNDADYPSSITLPVVPVAAISNLGATGPGCGDYWQVRCTTLRN"
BASE COUNT         1649 a         2681 c         2954 g         1899 t
ORIGIN      
        1 cgacccccgt ggtgtgagtc agatcgacga cccgctgttg atgtcctggg tcgatcttgt
       61 tgctctgggc cgtcaacacc gggaatcgga tccgggtgcg ctcgaggatc ttctggaagc
      121 ggagcccgat gacgtcatga cactgatcta cacctccgga acgaccggcc cccccaaggg
      181 agcgatgctc acgatccgta acgtggagtt cgcgatccag gaactcgtgg agggtggggg
      241 gttcacctcg cctccgccga ccaacagtga tgtcctgctg tcctaccttc cgctttgtca
      301 tgtcgccgaa cggatcttta ccgtttggtt caatctgtct gccggatgcg tcgtgaactt
      361 cgccgaatcc atcgagactg tgacccagaa cctgcaggag gttcagccga cgatcttcct
      421 cggcgtgccc cgcatctggg agaagatgca cgccggcatc cagatccgtg ccgccaacgc
      481 gtctcgtctc aagcgctgga acttcaggct ctggatgaag gtcgccgacc ggatcgctga
      541 tcgcctggtc gataccggag gcgtacacac gctcggcact cggatgctct attcaatcgg
      601 ctatgtcttt ctgttccgcg ccctcaagga gcggataggc atgctgcgat gccgctatgc
      661 ggcctcgggg gccgcgccga tcgccccgga gatcctgagg ttcttcatgg gcatcggcgt
      721 gcccatgcac gaggtttacg ggatgacgga gaactctgca gtggcgaccg ggaataggcc
      781 gggtcgcatc aagctcggga ccgttggcga gcctcacgcc aacgtcgagt tgaagatcga
      841 cgagcacacc ggcgagatcc tcacgcgcca ccccggcatc ttcgtcggct attggaacga
      901 ccccgaggca acggccgatg ccgtgactgc cgatggatgg ctccataccg gcgacgtggg
      961 ggagtgggtg gatggaaccc acgtgaagat aaccgaccgc atgaaagaca tcatcatcac
     1021 gtcgggagga aagaacgttt cgccttccga gatcgagaac tcgctcaaga cgtctccttt
     1081 catcaaggaa gccatagttg tgggcgatgg ccggaagtac ctcactgccc tggtcggcat
     1141 cgagatggac accgtcggca actgggcaca gcgccggggc atcacctaca cgacctatcg
     1201 caatctgtcg gagaaccctg aggtcgagaa actggtccgg ggcatcatcg atgacgtcaa
     1261 ccgaggcttc tcttcggtcg aacagatcaa ggctttccgg atgctgacca aggaactcga
     1321 tcacgaggac ggcgaactca cggcgaccca gaaggtgaaa cgagccgcga tcgccgggat
     1381 gttcggtgat ctcgtggagg agatgtacgg ggggaagccc acggcggcga gatcgacatg
     1441 acgagattca tccaggcgct cgtgatcgga ctggggctcg catccatcta ctcgttgctc
     1501 gcattggggt tcgtgatcat ctacaagtcg atgcgtgtga tcagctttgc ccaaccggga
     1561 ctgatgatcc tcggtgccta cttcgtcatc tacttcggga ccgtggtcga tctgagtttt
     1621 tggatcgcct tgcccctcgc tatggctacg gccgcgggcg caggattgtt gatagagcgg
     1681 ctggcgatcc ggcccatggt cggcaagcct gtcttcgccg tagcgatgat cacgatcggg
     1741 atcgacatcg ctctcagagt tctggttaac gatctcttgg gttcgaacgt gagagcggtc
     1801 ggggacccgt ggggcctagg tacggtcgaa gccttggggg tatcgatcca gcatcgacgc
     1861 atcgcgatgg tgatctttgc agtcgtcgtc gtgagtctct tgttcgcttt cttcaagtac
     1921 acgcgcatcg gtctcgcgat gcgagcaagc tcgttcgacc aggaggccgc cctcgcgcag
     1981 ggcatctcgg tgggatcggt cttcgcgttg tcatgggcgt tcgcgggagc cctcgcggcg
     2041 ttggcgggga tgttcgtcgg taccggtggg ggcatcgatc aaggatctgc cttcgtcgcg
     2101 ctcaaagcac ttccggcggt gatcctcgga ggtctcgact cgatatgggg cgcggtggtg
     2161 ggcgccatca tcatcggcat cttcgagggg ctgagcgtga cctatcaagg tcagtacctg
     2221 tcgttcctcg gtgggaactt ctcgcaggtc gtgccctatc tcgtgatgct cgtcgtcttg
     2281 ttggttagac cgtacggcct ctttgggaca ccggagatcg agcgggtatg agacgcaggc
     2341 cgcggatgta caccgcatac gaacaggatt cggccctgct caatacgtcg gccaagaaga
     2401 tatggatggc ttcactgctg gtgtgtttac tggtgctgcc gttcctgctg aacgatgcgc
     2461 tcgttgttct ggtcgcgacc gccttcgtcg catcgatcgg agcgatcggg ctcaacatcg
     2521 tgaccggata cgcgggccag gtgtccttgg gccacgcatt cttcttcgga ttaggtgcct
     2581 acacggcggc cgtccttggg ggagaccccg aggggcaact tgtcggttac ggagttgaca
     2641 tggaggtgtg gttgcctgct gccggcatcg ttgcggctct ggcgggtttg cttgtgtctc
     2701 cggtcgcttt caggttgcga ggcctgtacc tggcgatcgt gaccttggga cttgtgttca
     2761 tcggcgagca catcttcaga gaggcggaat ctctaacagg tggcacgggg gtcggccgta
     2821 cagcggcacg agctgtgctc ttcggtttcg cgtggaccga tcaggggcag atcgcgggca
     2881 tcgagttatc tcgcagccag ggcctgtatt tcatgagcct catcatcctt gtcatcctcg
     2941 ccctgccggc gcgcaacctg gcgcgctcgg cgatcgggcg cgctttcgct gccgtacgcg
     3001 accgcgatct tgctgccgag gcgatgggga tctccctggc caagcaaaag gcgcttgcgt
     3061 tcaccatctc gtccttctat gcgggcatag ccggcgcact cctcgcaacg atcactggtt
     3121 acctagagcc ggggagcttc aatcttctat tgagtgttcg ctacatcgcg atgatcttga
     3181 tcggcggagt agcgacgatc tcgggttcga taatgggtgc cgctttcgtc acgcttcttc
     3241 cccgcgccat ccaagagatc ccgcgtttcg tcccgataat cagcgaggct gctaccgggg
     3301 gtgtgcttac cacccagcag ctagaacaga tcatctacgg actgttgatc atctttttcc
     3361 tgatcctaga accgcgcggt ctttacgggc tgtgggtgag gattcgcaat tactggacac
     3421 aatggccgtt ctcttactga ccgagggagg tgaagcgggc acgcggttgt aagcgagatc
     3481 ctgacagtcc gtccgaaagg gaggctcgga tgaagggcaa gcttggttgg gtaagaacga
     3541 tcgctctgct gatgatgctc ggtgtcattg cgagcgcgtg cgcaagcggg ggagatgtcg
     3601 ccgaggagga tgacggcggc ggcgacgccg agatcgcaac cgacgtgggc gtgaccgaag
     3661 agccctgtcc aaaggcggtg aatgaagaca acggttgcat cttcttgggc acgttgtccg
     3721 acctcacgat cggtccgttc gcgccgctgg ccgtaccgat caccgattcg caggcggctt
     3781 tctggaaccg cgtgaacgaa gagggaggca tcggagacga gtacgacgtc gatgtcacga
     3841 cgcatgttca ggacaacgaa tacaaccccg aggttcacgt tcggaagtac cagcagatcg
     3901 aacccgacgt cctggcgctc gcccagaccc tcggcacgcc accgacgctc gccgcgctcg
     3961 acctctacaa gcaaggggac gtcgtgggcg cacccgcgtc gtggtggtcg ggctgggagt
     4021 tcgaggacgt catcatcgag tcggggacga actactgcct cgaatcgatg aatgcgatcg
     4081 actacgcgat cgaagagttc aactcaaaga ctgtgatggc cgtcggctat ccgggcgact
     4141 acggcggtga cgccgccgcg ggggccgaga tcgcggcgaa ggagaatgga ctcgaattcg
     4201 tcggcgcgat cgagacggca cctaacgccg tcgccggaag ccaagaggaa gcgatcggag
     4261 ccgtcctcga ggcgaacccc gatctcgtta tcgtgaccac gggaccgacg gagatggccg
     4321 agatcgtggg tggaaccgca gcgcgcgact tcaaaggcag gttcatgggc acgtccccga
     4381 cgtggaacaa gggtgtgctc gacagccctg cggcaccggc gatagaggct ctctatctac
     4441 agacggctcc gtgggctccg ttcgacaccg acacgccggg ccaccaagcg atgcgcgaag
     4501 ccgtcggcga cgtcgacccc aacgacggtt acacgtcggg ctgggtctgg tcgtatccgt
     4561 tgaggcaggt gctcgaggac gcgttcgcga gtggcgatct cactagagcc ggcttggtcg
     4621 aggccgcgtc cgcgctcgag accgtggact acgagggcat gctgccggag gaggcgggga
     4681 gattcgcggg ggacccgaac gacaccgtct tccgccagat cgtgatcaac aagcccaaca
     4741 agaaggcgcc ctcgggcgtc accttgctgg aggacttctt cgtcggtccg acggcggagg
     4801 cattcgaatt caccgagccg tgccaggcgg ccgaatgagc tgagaaagac cggtagctgg
     4861 agggcggaaa gccgctcttc agctaccgca acggggctag ccgcagcgtt tggggatcaa
     4921 gacctcgttc agatcggtcg gagggatccg cagtttcgtt gcgtgttcgt agtcgtatgc
     4981 gaacgagatc aatcgcggct cgctgaatcc gtccgccaag aagctcagac cgaatgggtt
     5041 ctgccctgcg tacccggccg gcacgatgac cgtcgggtat ccggctgcag cgctgacgtt
     5101 cgcgtatgtg ttacccggtg ccacgatcgc atcgagattg tcggctgcca gagtcgcgtc
     5161 gataaccgcc cgcgaggact ggatcgttgg ttccgcggtt gcggggtggt agacgcctgg
     5221 ctgcgcatcc gacgcgatca ggagattctg cccgtacttc accttgtccg gatgctcttc
     5281 gttgtaggca acgatgtcgc tcagcgttcg cactcgcagt gagtcgggcg tctgctcggc
     5341 gaggtactga ttcaatgaag ccttgaactc gttggggatc agaccgatct cagttagtcc
     5401 cgcaaggctc gtgttcgaga ggttgtccgt ctcgacgaga gttgcacctt gctcctcgag
     5461 cactgtcagt gctttctcga acaggccgtt gctgccgacg tcttgagggc tgtacccgag
     5521 ccgcactccc ttcagcgcgt tgtcatcgag gaacttcgag tagtccgacg gcgcgtctcc
     5581 actatcggcg gtgcggggat ccttatcgtc gacgccggcc atcgctccga gcatgatcgc
     5641 tgcgtcggtc acggttctcg tcatcggtcc gggcgtatcg aagctcggtg cgagcggcag
     5701 gatcccgaag cgactaagca gacccgtcgt cggctttatc ccaacgaggc tgttggccat
     5761 gctcgggctc aagatgctgc ccgaggtctc ggtccccacc gtgccggttg caaaggccat
     5821 ggaacctgca actccggaac cggagctaga tccgctcggg tcaccattcg tgtaagcgtt
     5881 gcggacctgg ccgccgagcg agctgtagcc gttgggcatg ctgagatcca cccaattggc
     5941 gaactccgac agattcgtct tgccgaggat gatcgccccg gcctcttcga gtttcgttgt
     6001 gatcgtcgcc gcctcgagag gaatgctccc tttgagcgcg atcgatcccg cggtcgtcgg
     6061 catgtcgaga gtgttgatgt tgtccttgag caggaccggg atgccgtgga gggggccgcg
     6121 cgtttctccg gagcgacgct cggcatcgag cttcccggcg atcttgagcg ccttgggatt
     6181 gagggcccgg atcgaattcg tgtgttcgtc gtaagcatcg attcgtgcga ggtaggccca
     6241 aacgagatct cggctagtcg tctttctgga agcgagagct tcttgcagct ccgggatcgt
     6301 cacggtttga agatcgagac ccgcaaccga gggagggcat tctttcgtcg gcgcggcgga
     6361 tgccgcgacg ctcgtggtac cggcggcaag cgccagacag gcgaccgcga tgagtgaggc
     6421 gagcagtctc cggccggatc tgggttccat gcctgaacat tcgcccaggg gcgcccgatt
     6481 cctacagaac gtcagctctt agggaggatg cccctgatga acgccgcctg gcccacgtgc
     6541 tgaagatcgt cctcgatcac gcttaccaga cgtaccccga gggtgacggg cgggtcccat
     6601 ctctcatcga cgatgcggtc gaggtcggat tcgctcagac ctcgtagata gtcgagtgtc
     6661 tggtcgtgca cggcgttgtg gtagccggtc aggagatcag acgacactcg tacagcggct
     6721 acctgatccg aggagtggcc gtatcccgtg tccgatgtgt cgagcggtag ggcgaagcgg
     6781 tctgaccatc ccgccgacgt ccacacttgt tcggtgccgg caacgtcggc gacatggtcg
     6841 tcctggacac gggtgaggtg ccagacaagc caggcgatcg tgttggcgtc gacgctcgcg
     6901 cggaactcca gttgttccgg gcgcagatcc ttaaccactc cgtggacggt ctcgtggacc
     6961 ctgccgaaag cgtccaccaa caggtcggaa acgttcaata cgactcctct cgatctcagc
     7021 ccatctgtag ccagctttcc tccgtctatt ttgtccgata tagtccctcg ttctgtaagc
     7081 aagagaagct cggaggagag ggattatgaa gaagatacgc gtcttactgg ttgcctgttt
     7141 ggctgcggcg ttcatggccg ttggggcggg cccggccagc gccaagtgcg tcggcgagcc
     7201 caacgtgtgt gtactggtct gtgaagttgg gaccagcaac aagtacaccg aagagctgtt
     7261 cgagttctgc tacgtctggt aggtcgacgc aaaacgtcgt aacggttaga actcaaaagg
     7321 gggcgcctcg gcgccccctt tttcagttca tccgagtgct agttcggatc taggcactgc
     7381 tcctgcctga tgacttcggc cacacattgc gctacatgct cccaggaaca ggctacgtac
     7441 tcatgatagt tatggatggt cgtgttgcag tggccggctt gggccggcgg cgccggcaac
     7501 acaactgcac cgatgaccat tgccgacata cttattacag cgagtcgctt cttcatgcac
     7561 acctccgtga tgccaacagt tagggaagtg gttctggcaa ggcgaggagt ctcctgctgg
     7621 cacaagagcg ccaactgcgg gtcgctaatt caagtaaggc gccgccgcga agcggcaagg
     7681 ggcgccgtct tgatcaatta cggagcgtag tgcagcgcac ctgccagtaa tcgccgcaac
     7741 ccggaccggt cgcacccagg ttgctgatcg cagcgacggg taccacaggc agggtgatcg
     7801 aggatgggta gtccgcgtcg ttgtagatga ccgtgttgat cgacgcgggc tggttcgatt
     7861 gcacggcgaa cgagtaattg ctgtcgatcg ccggcggcgc cgtaacgatc acaacgagtt
     7921 ggtggcccgg ccgaaacacg tgggccgtcg gccacacctc gatcttgtag tcgaccggct
     7981 catccggcgc cacgttctcc ggttctgtgt ggtgcctaaa cggccggtac atgaacccgc
     8041 ccgagtagtc gctgagtttc tcgtcgatgg cgtggtggct cgctttcaac caccctcggg
     8101 ttaggaactt gatctctttg ctttcggtgt cgaggtcggc aacctgcacg aacagatcgg
     8161 tgttgttccc cgtgatcgac atgcgcaggt cggcgatgat cggtcctgcg atggcccacg
     8221 tctcgtcggg gcccacgacc ggtccctgta ggaacacctg atcgggaccg tcctcgctgg
     8281 tgatgggggg gccgagatcg gggcccgcct ggaacaacca cgattgtcgc cgggttccgg
     8341 agaggtaact gtccgaaccc tcgccgcact gagttcggtc gaactccagg gccttccctg
     8401 cgcacaggta cgcggtcgtc cagttggttc cgtcgagcgg ccaacgcgtc gagtcgtagt
     8461 ggccgttgga gacgagagcg ccgtcctcat cgggatgaag ttcgaacagt gtccgcacgg
     8521 atgcccgcgt gatctttcca ctgggtgtgg ccatgtccca gcggggatcg ggtgtcacgc
     8581 cgcgcatcca gtagtccatc cacgctttgc ggtcggccca cgtctcctcg gctacgacat
     8641 tcgtgtcgtg gttgccgttc gattgcagga gtcgcttcgg gaggcccggt cgcacctctt
     8701 cccacaggtg cgccgtaccc ctagctcccg tctgctcgtc gttgaacgtg ccggttatgt
     8761 ggatcggaac gttgatcttg tcgatgtagg tgatcatcga gacgcggtgc cagtagtcgg
     8821 agtcaaggcc ttgcgacatg accccgttca ggatcggatc gttcgtcata tcgacggtgt
     8881 gcgtggcggt gttagccgca cagcgttgcc cgttctcgtt gtcgagattg cgcgctatgc
     8941 cctggccact tccgccgacg acgtgatagc cgttgcggat cccgaggtac cagagaggag
     9001 ggaacagcgt gttgagcacg ccacccggga acgtgatgcc ccgatagttg tcatcgacca
     9061 agcccgaaac ggtcatcgca gcgagatgct gcggctgatg cgacgcgatc agcattgccg
     9121 tggcgccgct gtaggagtga ccgagtaagc cgaccttccc gttcgaccat ggctgctgcg
     9181 gga
//
LOCUS       DATMED010000220         4156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_174996, whole genome shotgun sequence.
ACCESSION   DATMED010000220 DATMED010000000
VERSION     DATMED010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174996"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VNP73_05590"
     CDS             <1..936
                     /locus_tag="VNP73_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65430.1"
                     /translation="LQRIAIVSLLVAGLLAAASPAHALFHLMKITEVLAGTSDQSAAQ
                     FIEMQMYSDNQRFLATHEVVVYDGAGAEKATYTFSAPVENGTNQAHILLATPEAEAAF
                     GVTADLAMTPDLVAGGGKACFRSNDGGLIDCASWGNYSGDAEGSGTPFNSPIGLLADQ
                     SMQRKTSAGSNPDGLDAEDDTDDSAADFESGSPTPTNNAGEGGGGDDGGGGETDQQEH
                     ERAVTLTLKGSLKAKGEVSTADDYAACVSGVPVKVQRKKSGGWKTVKSTETDEAGAYT
                     VAMKNRPGRYRAKATPVTPEDGHECLPAASPVRKN"
     gene            976..1809
                     /locus_tag="VNP73_05595"
     CDS             976..1809
                     /locus_tag="VNP73_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWL65431.1"
                     /translation="MAKVAFVLGGGGRLGAAEVGMLRALFEAGITPELIVGTSIGAMN
                     GAAVAASPTLESVDRLEEIWTTLDKNEVFSGSLLSGAAHLVRTRTSVQSIAPLRKLIK
                     QMLPAETFADLEVPFQCVAASIERAAEHWFSEGPLVDAILASSAVPGILPAVKLDGEH
                     FIDGGIVNSIPIGRAVDLGATEIYVLHVGRIERPLTAPKNPWQVGMVAFEVARRHRFA
                     RDMSSLPEGVTAHVLPSGEEPPRYDSLGNLRYKNFKSVTKHINAAHEATVAYLKDGVT
                     A"
     gene            1809..2819
                     /locus_tag="VNP73_05600"
     CDS             1809..2819
                     /locus_tag="VNP73_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWL65432.1"
                     /translation="MGLPPKWVRRLLVAPLVLALCLALIALSPVFLLVAFVADLFVPG
                     SWRTVRMVAFAVFYLVMEVVGLVAMFVLWIRFGFGTRLKTERAVEAHYLFETWWLYRM
                     YRAVGKLFGLKINIEERPEPQPGPLLVFCRHAGPGNSLMLIGTMMIAYKRRPRIVMLA
                     KLQWDPLFDTMGNRLPNRFITHDKKDASRYTKAIGELAEGLRDHDAFVLFPEGRDFTQ
                     RLRVRAIDYLRERGFDKSAEKAEQMFYVLPPRHRGPLAAITAAPEADVAFVAHSVLEE
                     LGSFKELWRRIPLQHTVDAQYWRLTPDQVPRTEEEVIEWLYQWWERIDDWIARHKVLE
                     PV"
     gene            2820..3431
                     /locus_tag="VNP73_05605"
     CDS             2820..3431
                     /locus_tag="VNP73_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWL65433.1"
                     /translation="MEAKYFRRRLVVGAIVVALAVGAVTFFRRGEPLQPWQIRGSTAW
                     AVRHYGNPDAKRFKARHIVTIDFLGRAMFVHEESQRHFLRLERLFEARAPEYAAVVAA
                     GTLDDWSYENRDVRGEPGSKSKHSFGIALDVNALTNVLGTTGDMPEEVVRQWEIEGGE
                     WGGDWSRPDPMHFETHLTPREITRRYNPDGTVKDWYLEELVGG"
     gene            3424..4074
                     /locus_tag="VNP73_05610"
     CDS             3424..4074
                     /locus_tag="VNP73_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWL65434.1"
                     /translation="MANASLPRVVCFDVDGTLVPSTTVVLHLAEWLGFTEEFEELERR
                     YTEGEVSNGFVAERAAGYFAGISKTDAWDRLGSLPLIDGLEETLAWLRERSIHTLIGT
                     VTWDFAAEFLSDRYGFDAFCGCRMDTDEKGNLTGSIAQHIEAEEKAAFVREFCEEHGF
                     DLSQAAAVGDSRSDLPLFKEVGLAIALNGSPEADAAADVSIRTNDLRAVIPLLERR"
BASE COUNT          747 a         1288 c         1359 g          762 t
ORIGIN      
        1 ctgcagagga tcgcaatcgt ctcgttgctc gtcgcgggct tgttggccgc agcttcgccc
       61 gcgcacgcgt tgttccactt gatgaagatc accgaagtcc tcgcgggaac ctcggaccag
      121 tccgcagccc aattcatcga gatgcagatg tactcggaca accaacgatt ccttgccact
      181 cacgaagtgg tcgtgtacga cggcgcgggg gccgagaagg cgacgtacac gttttcggct
      241 ccggtcgaga acggaacgaa tcaagcgcac attttgcttg cgacccccga ggccgaggcg
      301 gcattcggcg tcaccgccga tctcgcgatg acccccgatc tcgttgccgg cggtggaaag
      361 gcttgtttcc gcagcaacga cggaggtctg atcgactgtg catcgtgggg caactactcc
      421 ggtgacgcgg agggcagcgg cactccgttc aattcaccga tcggacttct cgcggaccaa
      481 tcgatgcagc gcaagacgtc cgctggatcg aatccggatg ggctcgatgc cgaggacgac
      541 actgacgaca gcgccgcgga cttcgagtct gggtccccga cccccaccaa caatgccggt
      601 gaaggtggcg ggggcgatga cgggggcgga ggcgagaccg atcagcagga gcacgagcgc
      661 gccgtcacgc tgaccttgaa gggctcgctc aaggcaaagg gagaggtctc gacggccgac
      721 gactacgccg cgtgcgtgtc gggcgtgccc gtcaaagtgc agcgcaagaa gtctggtgga
      781 tggaagacgg tcaagtcgac cgagaccgac gaggcgggcg cgtacacggt ggcgatgaag
      841 aaccggccgg ggcgttaccg ggccaaagcc actccggtga cgcccgagga cggccacgag
      901 tgcctcccgg ccgcctcccc ggtccgcaag aactaacccg cgggggatct cagagcctct
      961 aactagtatc ggggcgtggc caaggtcgcg ttcgttctgg gtggaggcgg aaggctcggc
     1021 gccgccgagg tgggaatgct tcgtgcgctg ttcgaggccg gcatcacgcc cgagctcatc
     1081 gtcgggacct cgatcggtgc gatgaacggg gccgccgtgg cggcgagtcc cacgctcgag
     1141 tccgtcgacc gactcgaaga gatctggacg acgctcgaca agaacgaggt gttctccgga
     1201 tcccttctgt ccggggccgc tcatcttgtt cgcacccgaa cctcagtgca atcgatcgca
     1261 cctctccgca aactgatcaa acagatgctt ccggccgaga cgttcgccga cctggaagtc
     1321 ccgttccaat gtgtcgcggc ttcgatcgag cgcgccgccg agcactggtt ctcggagggt
     1381 ccgttggtgg acgcgatcct ggcgtcgtcg gcggtacccg gcatcctgcc ggcggtgaag
     1441 ctcgacggag aacacttcat cgacggtggc atcgtgaaca gcatcccgat cggtcgcgcc
     1501 gtcgatcttg gggcgaccga gatatacgtg ttgcacgttg gccggatcga acgaccactg
     1561 accgcgccca agaatccatg gcaggtcggc atggtcgcgt tcgaggtcgc gcgccggcac
     1621 cgcttcgctc gcgacatgtc atccctgccg gaaggcgtta ccgcgcacgt ccttccgtcc
     1681 ggggaagaac cgccccggta cgacagcctc ggcaacctcc gctacaagaa cttcaagtcc
     1741 gtcaccaagc acatcaatgc cgcgcatgag gcgaccgtcg cctacctgaa agatggagtg
     1801 acggcctgat gggtctgccc cccaagtggg tccgacgcct gcttgtcgca ccactcgtgc
     1861 tcgccctctg tttggcgcta atcgctctat cacctgtgtt cctgctcgtc gccttcgtgg
     1921 ccgacctgtt cgtgccgggc agctggcgca cggttcggat ggtggccttc gccgtcttct
     1981 atctggtgat ggaggtcgtc gggctcgtgg cgatgttcgt gctgtggatc cgcttcggct
     2041 tcggcacgcg tctcaagacc gaacgagccg tcgaggcgca ttacctcttc gagacgtggt
     2101 ggctctatcg catgtaccga gccgtaggga agctattcgg cttgaagatc aacatcgaag
     2161 agcgacccga gccgcagcct gggcccctac tcgtgttctg ccgccatgcc ggcccgggta
     2221 attccctgat gctgatcggc acgatgatga tcgcctacaa gcgcaggcct cggatcgtga
     2281 tgctggcgaa gctccagtgg gatccgttgt tcgacacgat ggggaaccga ttgccgaacc
     2341 gattcatcac gcacgacaag aaagatgcgt cccgctacac gaaagcgatc ggcgagctgg
     2401 ccgaggggct gcgcgaccac gatgcgttcg tgctctttcc cgagggtcgg gacttcacgc
     2461 aacgccttcg ggtgcgtgcg atcgactacc tgcgggagag gggtttcgac aagagcgccg
     2521 agaaggccga gcagatgttc tacgtgctgc cgccccgaca ccgcggcccc cttgcggcga
     2581 tcacggcggc ccccgaagcc gacgttgcgt tcgtcgccca ctccgtcctc gaagagctcg
     2641 gctctttcaa agagctgtgg cgcaggatcc cgctgcagca cacggtcgac gcccagtact
     2701 ggcgtcttac gccggaccag gtcccgcgca ccgaagagga agtcatcgag tggctctatc
     2761 aatggtggga acggatcgac gactggatag ctcggcacaa ggtgctcgag ccggtttagg
     2821 tggaagccaa gtacttccgc cgaaggttgg ttgtgggagc gatcgtggtt gctctcgccg
     2881 ttggggccgt gacgttcttc cgccggggtg agcccctgca gccgtggcag atccgtggct
     2941 cgacggcgtg ggccgtgcgg cactacggca accccgacgc caagcgcttc aaggctcgac
     3001 acatcgtcac gatcgatttc ttgggccggg cgatgttcgt gcacgaggag tcgcagcgtc
     3061 acttcctcag gctcgagcga ttgttcgaag ctcgagctcc cgaatatgca gcggtcgtcg
     3121 ctgccggcac cctcgacgat tggagctacg agaaccggga cgtccgggga gagcccggga
     3181 gtaagtcgaa acattcgttc gggatcgcgc tcgacgtcaa tgcactgacg aacgttctcg
     3241 gcacgaccgg tgacatgccc gaggaggtcg tgcgccagtg ggagatcgaa ggcggcgagt
     3301 ggggagggga ctggagtcgc cccgacccga tgcatttcga gacgcatctg actccgcgcg
     3361 agatcacgag gcgttacaac cccgatggca cggtcaagga ttggtatctg gaggaacttg
     3421 ttggtggcta acgcctcact tccgcgtgtc gtttgcttcg acgtggatgg gaccctcgtt
     3481 ccctcgacga cggtcgtgct tcatctcgcc gagtggttgg ggttcacgga ggagttcgaa
     3541 gagctcgagc gtaggtacac ggagggagag gtatcgaacg ggttcgttgc cgagagggcc
     3601 gccggctact tcgccggcat ctccaagacg gacgcgtggg accggctcgg gtcgcttccg
     3661 cttatcgatg gtctcgagga gacgctcgca tggctgcgcg agcgctcgat ccacactctg
     3721 atcggcaccg tcacgtggga cttcgcagcc gagttcctca gcgatcggta cggattcgac
     3781 gccttctgcg ggtgccggat ggacaccgac gagaagggca acttgacggg ttctatcgca
     3841 cagcacatcg aggccgagga gaaggccgcg tttgtgcggg agttctgcga ggagcacgga
     3901 ttcgatctgt cccaggccgc tgcggtcgga gactcacggt ccgaccttcc actgttcaaa
     3961 gaggttggtc tcgcgatcgc tctcaatgga agtcctgaag cggatgccgc agcagacgtc
     4021 tcgatccgaa cgaacgatct acgcgccgtg atccctctgc tcgagaggcg ctaacggagc
     4081 ttcggccccc ccacgatcgt tccgacgatc ccttcgttcc cctggttgcc ggactcgccg
     4141 gtgacgcagc ccatga
//
LOCUS       DATMED010000221         3582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4390275, whole genome shotgun sequence.
ACCESSION   DATMED010000221 DATMED010000000
VERSION     DATMED010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4390275"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VNP73_05615"
     CDS             complement(<1..642)
                     /locus_tag="VNP73_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWL65435.1"
                     /translation="MVGRPEALTRPSSRDGFSPAVPALLGAMTVWGASYVVTKSALAA
                     AGPVTVLFLRLAIAAVVLAPFAVRKGFGLRDIFRTRFALFGLTGMALHLGFEIVGLEF
                     TSASSAALVIATAPAVTVAFSVILLRERLSLARWVGLAFSVAGVVLVTGGQSAAGYPL
                     AWLGNLLVFAGVVTWGVFTVQGKRLSEGYHWLVSTTAATAFAAILVAPVMVGEI"
     gene            846..1400
                     /locus_tag="VNP73_05620"
     CDS             846..1400
                     /locus_tag="VNP73_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWL65436.1"
                     /translation="MDFTHYTDLCVHAAADLVNTQGSISGNEFLGTPEAVAEFLASHG
                     FSETEGATPADMKEIHAVRDRLREVFLADDAKAAAELLNGILDELDVHPYLTDHDGVW
                     HFHYVPTGASLAVRVAATTAMGLATVLSEFGFDRIGFCQAENCKDVFVDMSRNRSRRY
                     CNEICSSRMNVAAYRARHKQKAAQ"
     gene            1405..1911
                     /locus_tag="VNP73_05625"
     CDS             1405..1911
                     /locus_tag="VNP73_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HWL65437.1"
                     /translation="MLRFVVQKHAATSLHYDFRLEVDGVLKSWAVPKGPSLDPRDKRL
                     AMPVEDHALDYIDFEGVIPEGSYGAGPVIVWDRGTYVNLTEKDGAEIPLSAALKDGYA
                     SVWLKGNKLIGGWSLRRVSIGRDAREKWLLVKKRDETADDSRQPTSDQPGSVLSGRTI
                     EQLAEEDA"
     gene            1994..2695
                     /locus_tag="VNP73_05630"
     CDS             1994..2695
                     /locus_tag="VNP73_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HWL65438.1"
                     /translation="MEKGKQRLLELLAEAHANELALINTLEAHVRIADRGSYRSLLQD
                     HLRETKSHAAQIKRRLDRLGYSESLFARTYGLAQNLIKQSLVLTKGPIDMVRGGSDIN
                     EKMLRNARDEVTTEALEIACYDTLESLARGLGDHETAELAATIRLDEERMLDSLRKEI
                     PILTDLVVTTTLAEGDKQIEEPWSGYDDMTVDQIKSRLSEATPSLLVAVRMYEEDNKN
                     RTTVIEATEKESISV"
     gene            2806..3507
                     /locus_tag="VNP73_05635"
     CDS             2806..3507
                     /locus_tag="VNP73_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65439.1"
                     /translation="MAMDVEARIDELFSLPLEDFTAARNKLATELKKDDPDGAARVKA
                     LKKPTSAVWAINQLAHRHGPELEELVRLHERMMSIKDADDLRAASKERRRVVGRLTDL
                     ARGELASAGQKGTTATSQRITQTLIAAASGEELDALRWGRLTADIDSPGFQAMAGFEA
                     AGDSALYDKPHKRAAARREALEKEFEEAEKTADELTRSAEKAQWEADRLAAQAEKARS
                     HATSVRAKLEASEDA"
BASE COUNT          767 a         1080 c         1158 g          577 t
ORIGIN      
        1 gatctcgccc accatgacgg gcgctacgag gatcgccgcg aacgcggttg cggcggtggt
       61 ggagaccagc cagtggtacc cctccgacag ccgctttcct tgcactgtga agactcccca
      121 agtgacgacc ccggcgaaga cgaggaggtt ccccagccat gccaacggat agccggcggc
      181 ggattggcct ccggtcacga gtacgacccc cgctaccgag aaggcgaggc cgacccagcg
      241 cgcaaggctg aggcgctcgc gcaggagtat cacggagaag gccaccgtca ccgccggtgc
      301 cgtggcgatc accaacgcgg cgctggaagc cgacgtgaac tcgagaccca cgatctcgaa
      361 gccgagatgg agcgccatcc ccgtgagacc gaagagcgcg aaacgcgtgc ggaagatgtc
      421 ccttaggccg aagcccttgc ggacggcgaa gggagccagg accacggcag cgatcgccag
      481 tcgtaggaac aagaccgtga ccgggccggc cgcggccaga gcggacttcg tgaccacata
      541 gctggccccc cacacggtca tcgcaccgag caatgccggg acggcggggg agaacccgtc
      601 ccggctgctc ggtcgagtta aggcttcagg acgtccgacc actaatgagc ctcgggatgt
      661 gctttccgcg gcttgcggcg cagagccagc tcgatcaggc gctgtcggca gccggagagg
      721 cgtacttcct tggcccgtag ggcggataat tcttctctga tggacgggac cattgctttc
      781 acctccgtaa gggtctacac tctctatacc tcttacaccc taatagggga ggagtaagaa
      841 gtcaagtgga tttcacccat tacaccgacc tgtgcgtgca cgccgcggcc gacctggtga
      901 acacccaggg atcgatcagt ggcaacgagt tcctgggcac tcccgaggcg gtcgccgagt
      961 tcctggcctc ccacggtttc tccgagacgg agggggcgac cccggccgac atgaaggaga
     1021 tccacgcggt acgggaccgc ctcagagagg tgttcctcgc cgacgatgcg aaggccgcgg
     1081 ccgagcttct caacggcatc ctggacgagc tcgacgtcca cccctacctg accgatcacg
     1141 atggggtgtg gcacttccac tacgtgccga cgggggcctc gttggccgtc cgtgtcgccg
     1201 ccaccacggc gatgggcctg gccaccgtcc tctccgagtt cgggttcgac cggatcggtt
     1261 tctgtcaggc cgagaattgc aaggacgtct tcgtggacat gtcgcgcaac cggtcccgcc
     1321 ggtattgcaa cgagatctgt tcttcccgca tgaatgtggc cgcctaccgc gcccggcaca
     1381 agcagaaggc cgctcagtag tcccttgctt cgcttcgtcg tgcagaagca cgcagcaacg
     1441 agccttcact acgacttcag gctggaggtc gacggcgtgc tcaagtcgtg ggccgttccc
     1501 aaagggccct ctctcgatcc acgcgataaa cggctggcga tgccggtcga ggatcacgct
     1561 ctcgactaca tcgacttcga aggtgtcatc cccgaaggga gctatggggc gggtccggtg
     1621 atcgtgtggg acaggggcac ctatgtgaac ctgaccgaga aagacggcgc ggagatccct
     1681 ctaagcgctg cattgaaaga tggctatgca agcgtgtggt tgaagggaaa caagctcatc
     1741 ggcggttggt cgctgagacg cgtgtcgatc gggcgcgacg cacgagaaaa atggttgctc
     1801 gtgaagaaga gagacgagac tgcggacgac agtcggcaac cgacatcaga ccaaccggga
     1861 tctgttctca gcggccggac gatcgaacag cttgcggaag aagacgcgta ggtttttcgc
     1921 gcactcgacg ccgcgattgc tcatccggct cgatgggtat cactaacgtt cgcctaacaa
     1981 caggaggttt cctatggaga aaggcaagca gagattgctc gagctgctcg cggaggcgca
     2041 tgccaacgag ctcgctctga tcaacacgct cgaagcgcac gtccgcatcg cggatcgcgg
     2101 ttcgtaccgc tcgctgcttc aagaccatct gcgcgagacg aagtcgcatg cagcgcagat
     2161 caagcgcagg ctggaccgcc tcggctatag cgaaagcttg ttcgccagga cctacggact
     2221 cgcgcagaac ctgatcaagc agagtctcgt gctcacgaag ggcccgatcg acatggttcg
     2281 cggtggcagt gacatcaacg agaagatgct gcgtaacgcc cgagacgagg tgaccaccga
     2341 ggcgctcgag atcgcgtgtt acgacacgct cgaatcgctc gcacgaggcc tgggcgacca
     2401 cgagaccgcc gaattggcgg cgacgatacg tctcgacgag gagcggatgc ttgattcgct
     2461 tcgcaaggag atcccgatcc ttaccgacct cgttgtaacg acaacgctgg cagaggggga
     2521 caagcagatc gaggagccgt ggagcggcta cgacgacatg accgtcgacc agatcaagtc
     2581 ccgcctaagc gaagcaaccc cgagccttct cgttgcggtg cgcatgtacg aagaggacaa
     2641 caagaacaga acaaccgtta tcgaagcaac cgaaaaggaa tcgatctcgg tttaagtaac
     2701 agatgcgagg gggatagata cccctgcagc tctcgaagag gcgcggtccc cgctggccgc
     2761 gcctcttctt tttctcgagt gaaggaggct gggtagcctc gcgacatggc gatggatgtc
     2821 gaggcgcgga tcgatgaact gttctcgctt cctctcgagg acttcaccgc ggcccgcaac
     2881 aagctcgcga cggaactgaa gaaggacgat cccgatggtg ctgcacgcgt taaagcgctg
     2941 aagaaaccga catctgcggt ctgggcgatc aatcaactcg cgcaccgtca cggcccggag
     3001 ttggaggaac tcgtgcgctt gcacgaacgg atgatgtcga tcaaggatgc cgacgacctg
     3061 cgcgctgcat cgaaggagcg ccgacgagtc gtgggacgac taactgatct tgcacggggg
     3121 gagcttgcat ccgcaggaca gaaggggacg acggcgacct cgcagcggat aacccagacg
     3181 ttgatcgcgg cagcttcggg cgaagagctc gacgcgctgc gatggggtcg gttgacggcc
     3241 gatatcgaca gtcccggttt ccaggcgatg gcgggattcg aggccgccgg cgacagcgcg
     3301 ctctacgaca agccccataa gcgagcggcg gcccggcggg aagcactcga gaaggagttc
     3361 gaggaggccg agaagacggc cgatgaactt acgcgatcgg cggagaaggc ccagtgggaa
     3421 gcggaccgac tcgccgcaca agcagagaag gctcgcagcc acgcaacctc ggtgcgcgcc
     3481 aaactggaag catcggagga cgcctaagcg gctccgtttg attgcatcgg gtctggggta
     3541 atccatatga acccctgcgg tgcgggatgg gaggacaccc ca
//
LOCUS       DATMED010000222         4233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4390758, whole genome shotgun sequence.
ACCESSION   DATMED010000222 DATMED010000000
VERSION     DATMED010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4390758"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VNP73_05640"
     CDS             <1..1139
                     /locus_tag="VNP73_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWL65440.1"
                     /translation="FLGRDLEGATLDDLQIARVRYDPGERIIVHYRATENGHGLDAVA
                     TAIARQNLGAKVQNPRFTTMATIVNGRSPGRRPVAHDPEVDAIIAWLPFDAKLPGLAI
                     PPHELVDRLTRAGVPVGDNREPVLVGYKPSSRAVLRLGDQILKAYGKDSHFEAARNGL
                     LASGSSPSLCTVQLRAEFDDLRLTSQAAVDGTTPSEALDVAYESGEFLRILQNQEFND
                     LPATSPEHQKQEADRHARLASVLVPDLSPQLERLIERLNQSMPEDVELTPAHGDFHVD
                     QLIANEERLTVIDFDEMCRAPAALDIATYAADVVRGCDQDLERAHATLQPLCEGYADT
                     PAWIEWYLSTAILCRATRPFRSQSSNWPERTRAIVRAADGALSS"
     gene            1136..2476
                     /locus_tag="VNP73_05645"
     CDS             1136..2476
                     /locus_tag="VNP73_05645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL65441.1"
                     /translation="MNSTPAGAECSIAYVLKGFPVLSETFISSEIHRVEKAGINLRLF
                     VVKPRDQREHHPVVDRIQAQPDYLPPTSPISETSRTRWLLNNAPSFVPSFSRALRRKP
                     TGTLGAIRFTLVELVSTRKSLWSWPKVSCIRDLFLALALSDRILSAPDVRHLHAHFAH
                     DTTTITWLAAMITGLPFSFTAHAKDIYAARLNPRDLLRRKVNAASFVVTCTEANRKHL
                     SAMANGTPIHRVYHGLNDDFWRFSQRSVLSERPHENLRLLAVGRLVAKKGLDTFVEAC
                     GLLDRQGLAFEATIIGEDDDVGDNFSVGEDLRRRIARLGLEDRVHMLGQIGQAELYDH
                     YRDATVFCLPCRVLENGDRDGIPNVLLEAMSCGVPVVSTPVSGIPELVEDGTNGLLVP
                     QDDPEALAAALRKLHENSDLVSRLGHAGRATVRERFDGDILSRYLATLFERATA"
     gene            2476..>4233
                     /locus_tag="VNP73_05650"
     CDS             2476..>4233
                     /locus_tag="VNP73_05650"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HWL65442.1"
                     /translation="MEQVPLLLADAEEQTAPRRVFCITRDVHNDKALADAVCGGRFTH
                     AGVTLDLGPEPDWLSDDLPADEEWRIEWSKFYYGLDLANAFVATGDLRYLRVWERLAG
                     SWIRQVPIGFDTSDVAARRMQNWTYAWSLFTTSPAFPGLHEGLDAAIVESISEHARFV
                     RQALTPDVSRNHRTLELYALFVVAVAFPVIDHDSRLADFALSELNRILAEGFRSDGVH
                     RENSTHYHLVALRSLVGARENARRFSLEIPHEYDSLLERACEFALHCHRPDGVVSALS
                     DADSASYPEVLELAATLLGRPDFRYAPTKGARGTPPRKRNISFEDSGYFFQRSGWGMS
                     GSPYSDERFLVFDCGPLGDGGHGHYDLLSMEAYAYGRPLVVDPGRYTYAEEGENLRRL
                     FKGTAAHNTVCIDGLDQTPYRRGRPGKRIASAHFLGRRSVPGLDLLEGRAESPCYDAV
                     HTRRILFVGDEYWIVEDQLSAERKHLYDLRWHLTPEAMGATEAQQVNGNSVVRAPGVT
                     LVFPGRASVDLEPGWVSPRYGTRLPAPVVSASLRGTHARFVTLIVPMRSGRPEPRARF
                     RRTEGAKDACVLELEGVGVG"
BASE COUNT          804 a         1351 c         1281 g          797 t
ORIGIN      
        1 gcttcctcgg gcgcgacctc gagggtgcca cgcttgatga tctccaaatc gccagggttc
       61 ggtacgaccc cggcgaaagg atcatcgtcc actaccgcgc aacggaaaac ggccacggcc
      121 tcgatgcggt cgcgacggcg atcgcccggc agaacctcgg agcaaaggtt caaaatcccc
      181 gattcacgac catggcgacc atcgtgaatg gacgctctcc gggtcgccgg ccagtggcgc
      241 acgacccgga ggtcgatgca atcatcgcct ggcttccgtt cgacgcgaag ctaccgggac
      301 tggcaattcc gccacacgag ctcgtcgacc ggctgacgcg agccggtgtc cccgtcgggg
      361 acaaccgaga gccggttctc gttggctaca agccctcgtc tcgggcggtt ctccggctcg
      421 gagatcagat actcaaggcg tacgggaagg acagccattt cgaggccgcg cgcaatgggc
      481 tactcgccag cggaagttcg ccgtcgctct gcaccgtgca actgcgagcc gagttcgacg
      541 atctaaggct tacatcccaa gcggcagttg atggaacaac tccctcggag gccctcgacg
      601 tcgcctatga gtccggtgag ttcctgcgga ttctgcagaa tcaagagttc aacgaccttc
      661 ctgccacctc tcccgagcat caaaaacaag aggctgatcg gcacgcgcgt ctggcaagcg
      721 tgctcgtgcc cgacctaagt ccccaactag agcgactgat cgaacgactg aatcaatcga
      781 tgcccgagga cgttgaacta acccccgcgc acggcgactt tcatgtcgac cagttgatcg
      841 cgaacgagga acgtctgacg gtcatcgact tcgacgagat gtgcagggcc cccgctgccc
      901 tggacatcgc gacatacgct gccgacgttg tacgaggttg cgaccaagac ctcgagcgcg
      961 ctcacgcgac gctgcaacca ctctgcgagg gctacgcaga cacgccggcc tggatcgagt
     1021 ggtatttgtc caccgcgatc ctgtgcagag cgacccgtcc gtttcgatct cagtcatcga
     1081 attggccaga acgcaccaga gcgatcgtcc gcgcggcaga tggcgcgttg tcgtcatgaa
     1141 ttccaccccc gcgggagccg aatgctccat tgcctacgtg ctcaaggggt tcccggtcct
     1201 ttcggagacc tttatttcca gcgaaatcca tcgcgttgag aaggccggaa tcaacctgcg
     1261 actctttgtc gtcaagccgc gcgatcagcg cgagcaccat cccgtcgtcg accgtatcca
     1321 agcacagccg gactaccttc ctccaacaag ccccatctcg gagacctcgc gcacgcggtg
     1381 gctgctgaac aatgcaccgt ccttcgttcc cagctttagt cgcgcgctgc gacgcaagcc
     1441 aactggcacc ctaggagcaa tcaggttcac gttggtcgag ttggtctcga cgcgcaagtc
     1501 tctatggtcg tggccaaagg tcagttgcat ccgggatctc ttcttggctc tggccctctc
     1561 ggatcgaatc ctcagtgccc ctgacgtgcg gcacctgcac gcacacttcg cccacgacac
     1621 caccacaatc acctggctcg ccgcgatgat caccggactc cctttctcgt tcaccgcgca
     1681 cgcaaaggac atctacgcag cgcggctgaa cccgcgcgac ctccttcgca gaaaggtcaa
     1741 cgctgccagt ttcgtggtca cctgcaccga ggcgaaccgc aagcatctca gcgctatggc
     1801 aaatgggaca ccgatccacc gcgtgtacca cgggttgaac gacgatttct ggaggttctc
     1861 gcagcgttcc gttctgagtg aacggcccca cgagaacctg cgcttgctgg cggtcgggag
     1921 gttggtcgcc aagaagggtt tggacacctt tgtcgaagcg tgtgggttgc ttgatcgaca
     1981 aggcctggcc ttcgaagcaa cgatcatcgg agaggacgat gacgttggcg acaatttcag
     2041 tgttggcgag gacctccggc gccggatcgc gcggctcggg ctcgaggacc gggttcacat
     2101 gctgggccag attggccagg cagagctcta cgaccattac agagatgcga ccgtcttttg
     2161 ccttccctgc cgcgtactgg aaaacggaga ccgggacggc atccccaatg tcctgttgga
     2221 ggcgatgtcc tgcggtgtcc ccgtagtaag cacaccggtg tccggtatcc cggagctggt
     2281 cgaggatggg accaacggtc tgctggttcc gcaagacgat cccgaagctc tcgccgccgc
     2341 gctgcgaaaa cttcatgaga actccgacct ggtttcgcgc ttgggtcacg ccggcagggc
     2401 gacggtgcgt gagcgattcg atggggacat cctgtcgcgg tacctcgcga ccttgttcga
     2461 aagggcgacg gcttgatgga acaggtccca ctgctactcg cggacgcaga agagcaaacc
     2521 gcgcctcgtc gggtgttctg catcacgagg gacgttcaca acgacaaggc tcttgccgat
     2581 gctgtgtgcg ggggacgctt tactcacgcg ggcgtcaccc tcgatctcgg acccgaaccc
     2641 gactggttga gtgatgacct tccggcggac gaggaatggc ggatcgagtg gagcaagttc
     2701 tactacgggc tcgatctggc gaacgccttt gtagcgaccg gtgacctgcg gtacctgcgg
     2761 gtctgggagc gcctcgctgg gtcgtggatt cgacaggtcc cgattgggtt cgatacgagc
     2821 gatgtcgcgg cgcgaaggat gcagaactgg acctacgcct ggagtctctt caccacctcc
     2881 ccagcgttcc ccgggctgca tgagggattg gatgctgcca tcgtcgagag catctccgag
     2941 catgcgcgct ttgtgcgaca agcgcttacc ccagatgtat cgaggaacca ccgcactctc
     3001 gagctgtacg cgctcttcgt cgtcgcagtt gccttcccgg ttatcgatca cgattcgcgg
     3061 ctcgcggact ttgcgctctc ggaattaaac cgaatcctcg ccgaggggtt ccgatctgac
     3121 ggcgtccacc gtgagaactc gacgcactat catctcgtcg ctctccggtc gcttgtggga
     3181 gcacgggaga acgcgcggag attctctctc gaaatcccac acgaatatga ctctctactc
     3241 gagcgcgcct gcgaattcgc acttcattgc cacagacccg acggcgtggt ctccgcgctg
     3301 tccgacgcgg acagtgcctc gtaccccgag gtgctggaac tggccgctac gttgctgggt
     3361 cgacccgact tcaggtacgc gccgaccaag ggggcacgag ggacgcctcc ccgaaagcga
     3421 aacatcagct ttgaggacag cggctacttc tttcaacgaa gcggctgggg catgtccggg
     3481 agtccgtatt ccgacgagcg ctttctcgtc ttcgactgtg gcccccttgg cgatggagga
     3541 cacggtcact atgacctgct gagtatggag gcctacgcgt acggtcgtcc cctggtcgtg
     3601 gatcccggcc gatacaccta tgccgaggaa ggcgagaacc ttcgtcgctt gttcaaaggg
     3661 accgcggcgc acaacacggt ttgcatcgac gggctcgatc aaacgccata ccgcagaggg
     3721 aggccaggaa aacggatcgc gtccgcacac ttcctcgggc ggagaagtgt tcccggactc
     3781 gacctgctcg agggccgagc cgaaagcccc tgctacgacg cggttcacac ccggcgcatc
     3841 ctcttcgtcg gagacgagta ctggatcgtc gaagaccaac tgagtgccga gcgaaagcat
     3901 ctctacgacc tgcgttggca cctcacgcct gaggcgatgg gcgcaaccga agcgcagcag
     3961 gtcaacggga attcggtcgt gcgggccccg ggtgtcactc tcgtgttccc gggacgcgcc
     4021 tccgtcgatc tcgagccggg ctgggtctcg ccccgctacg gcacgagact cccagctccc
     4081 gtggtgagcg catcattgcg cgggacgcac gcgcggttcg tcaccctgat agttccgatg
     4141 cgaagtggcc ggcctgagcc gagagcgcgt ttccgccgaa ccgagggtgc gaaggacgct
     4201 tgcgtccttg agctcgaggg cgtgggcgtg ggc
//
LOCUS       DATMED010000223         4888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1708180, whole genome shotgun sequence.
ACCESSION   DATMED010000223 DATMED010000000
VERSION     DATMED010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708180"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VNP73_05655"
     CDS             <1..145
                     /locus_tag="VNP73_05655"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HWL65443.1"
                     /translation="PDNETHEVRGLFVADASCFPTASGVNPMVSIYGIAHRAAQRIAA
                     RIS"
     gene            202..1170
                     /locus_tag="VNP73_05660"
     CDS             202..1170
                     /locus_tag="VNP73_05660"
                     /inference="COORDINATES: protein motif:HMM:NF039678.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6183 family protein"
                     /protein_id="HWL65444.1"
                     /translation="MRAVDGLCAARDWDGMEDLAHACMDAVERGKQLWPITAQIDYRL
                     ALEAPGDHAAEVLESDLGRFLPGPLTEVAASTHTWEEMAPYIESPQAAAYVAQERVLR
                     GEVLTGDERAHQEVVDLPLALEEFEPTYALATFTAHDAEVAEPWEPKGTPVPLDAAPV
                     NPVPGAGIIEVLLDIVQPWTNESNGAARAVLVEADAMSAIATLTDGIPEPIDLTPLEP
                     EEALQRIAWAAASGGAHGRRRGAAFGRFTAWAVVASLAGRRWPVAAQEMGAALRSFEW
                     FWWDDRELKEGWVFRLAVQDASAGWAAAIGATDLLEEELEEGLEEG"
     gene            complement(1167..1889)
                     /locus_tag="VNP73_05665"
     CDS             complement(1167..1889)
                     /locus_tag="VNP73_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWL65445.1"
                     /translation="MADLPDGVKITWAGHATFIIETPSKKRLIVDPWLKTSPVCPDDL
                     KDPGPLDAILLTHGHGDHIADAIELADSTGATVVAMVEVAAWLNSKGVDEGKLIAMNK
                     GGTVEVAGCRVHMTHAIHSSSMEDGDKLVYGGEPAGFVIEIENGYKIYHSGDTAVMSD
                     MALIGRLLEPDIALLCIGDHFTMGPRSAAEAIRLLGVKTVIGMHYGTFPLLTGTPEQL
                     RLEAKDVDGLEVIELEPGQSIG"
     gene            complement(1953..3116)
                     /locus_tag="VNP73_05670"
     CDS             complement(1953..3116)
                     /locus_tag="VNP73_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL65446.1"
                     /translation="MEFTIPAEYSELQKNLTGFIDRELRPLEEEHLDPEVDDVPLELR
                     DRIRRRSAELGFYAADFPEEVGGSGLPQLGMVLLREAAARTGCRLAAFTTYGPEGPTG
                     LLLNGTAEQKKEYLVPLVTAEKSMCFCLTEPDAGSDAQNIKTTATKDGDQWVLNGTKH
                     FITNGKHADFALVFAANDRAKKAQGGISAFIVEKGTPGFEVGRAQKGMVEGEGQFELI
                     FKDCRVSEEQILGGPDKVGMGFYSAMQFLAMGRLSIASMCNGIADYVLSLGIDYANQR
                     VAFDKPIGKNQYVQGHIVESFIELKASKLMTYECAWKYDAGDPVIQESSIVKYYASEM
                     VNRVVDRMIQVHGGMGWMRELPLERIYRLVRIFTLVEGTSEIQKYIIAKTLGL"
     gene            3243..3875
                     /locus_tag="VNP73_05675"
     CDS             3243..3875
                     /locus_tag="VNP73_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65447.1"
                     /translation="MTTVFVLEDHQLVREAIADYLSSHGMEIVGQAATAGEGLTGITT
                     TQPDVALIDVGLPDGNGMEVCREVASRHPDVRCLMITGLTDDSLVAEAVLAGAYGFVT
                     KTIALDDLLGAVEKVAEGVPMVDPSAAYSLIENLRSRPSDEGDEAELTRQESRTLDLI
                     AEGLTNREIAERLQLAEQTVKNYVSNILTKLGFERRTQAALYAARKRDLN"
     gene            complement(3872..>4888)
                     /locus_tag="VNP73_05680"
     CDS             complement(3872..>4888)
                     /locus_tag="VNP73_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65448.1"
                     /translation="GSDLWARAFVQKRAIVDYLGSDDGSEDALPALDQLARIFETNGD
                     RLWLGRAEYQKANLHWDRYELSATEAALERALICARVAESPCDIARVLGSMTAVLLLG
                     PQPVPEAIIRCEEIIALPETSLLVKAKSLLRLAGLKAMQEEFPTARGLIEEGRSLLQD
                     LGHNFALAASSHEAGMVEVLAEDHAAAEAEFRAGIAKLEAMQEKATLASSAAFLSRVL
                     YTQERYDEALEWTWTSEAAWNGDSLGIVEWGPTRAKLTARAGDIDEAKALALAALEPV
                     ASSDDILTRTHSLLGVADVLLIAGRNRAALPYLEEVVALYRRKGILSLEKSILRKLDA
                     AIAA"
BASE COUNT          890 a         1558 c         1520 g          920 t
ORIGIN      
        1 tccggacaac gagactcacg aggttcgagg tttgttcgtc gccgacgcgt cgtgtttccc
       61 cacggcttcc ggcgtcaatc cgatggtctc catctacggg atcgcgcacc gggccgcgca
      121 gcggatcgcc gcccggatct cttaatcccc ctggacgaca agctccgcga cctcatcgag
      181 gcagccgacc tcaacggact cttgcgcgcc gtcgacgggc tgtgtgctgc gcgtgactgg
      241 gatgggatgg aggatctcgc gcacgcgtgc atggacgcag tcgagagggg aaagcagctg
      301 tggcccatca cggcgcagat cgattacaga ctcgcgttgg aggcgcccgg tgatcacgca
      361 gccgaggtgc tcgagtcgga tctagggcgt ttcctgcccg gccccctcac ggaggtggcg
      421 gcttcgaccc acacgtggga agagatggcg ccctacatcg agagtccgca agcggccgcg
      481 tacgtggctc aagaacgtgt tcttcgtggt gaggtcctta cgggcgacga gcgcgcgcac
      541 caagaggtgg tcgacctccc cctggcattg gaggagttcg agcccacgta cgccctcgca
      601 actttcacag ctcacgatgc ggaggttgca gaaccgtggg aaccgaaggg cactccggtg
      661 ccgctggacg cggcgccggt gaatccggtc ccgggggcag gcatcatcga agttctgctc
      721 gacatcgtgc agccgtggac gaacgaatcg aacggtgccg ctcgtgcggt gttggtggaa
      781 gccgacgcga tgagtgcgat cgcgacgctg accgacggga tccccgagcc catcgatctc
      841 accccgctgg agccggagga ggcactgcag cggatcgcgt gggccgccgc cagtggtggc
      901 gctcacggtc gtcgccgggg ggccgcgttt ggacgcttca ccgcgtgggc ggtggttgca
      961 tcgctcgccg gaagacggtg gccggtggct gctcaggaga tgggggccgc actgcgatcg
     1021 ttcgagtggt tctggtggga cgaccgggag ctgaaggagg gctgggtgtt caggctcgcg
     1081 gtccaggacg ccagcgcggg ctgggccgcc gcgatcggtg cgacagacct acttgaagag
     1141 gaacttgaag agggactgga ggagggctag ccgatcgact gaccgggctc caactcgatc
     1201 acctcgaggc cgtcgacgtc cttcgcttcg aggcgaagct gctccggggt tccggtcaac
     1261 agcgggaagg tcccgtagtg catcccgatg accgtcttga cgcccagcag ccggatcgct
     1321 tcggctgccg accgcggtcc catcgtgaag tgatcgccga tgcagaggag ggcgatgtcc
     1381 ggctcgagca ggcgaccgat cagtgccatg tcggacatca cggcggtgtc acccgagtga
     1441 tagatcttgt aaccgttctc gatctcgatc acgaagccgg ccggttcgcc gccgtaaacg
     1501 agtttgtcgc cgtcctccat gctgctcgag tggatcgcgt gcgtcatgtg gacgcggcag
     1561 ccggcgacct cgacggtgcc gcctttgttc atcgcgatca gcttgccctc atcgactccc
     1621 ttggaattca gccacgcggc gacctcgacc atcgcgacga cggtcgcccc cgttgagtcg
     1681 gccagctcga tcgcgtcggc gatgtgatct ccatgaccgt gtgtgagcag gatcgcgtcg
     1741 agggggccgg gatccttcaa gtcgtcgggg catacggggc tcgtcttcag ccacgggtcg
     1801 acgatcagac gcttcttcga cggggtctcg atgatgaacg ttgcatgtcc cgcccacgtg
     1861 atcttcacgc catcgggtag gtcagccatg tgcgcgcccc tttctagtcc cgccgaacgt
     1921 ctagacgttc ggcgctcggc caagtctcca gttcataagc cgagtgtttt ggcgattatg
     1981 tacttctgta tctccgatgt gccctcgacc agagtgaaga tgcggaccag ccgatagatg
     2041 cgctccagcg gcagctcccg catccagccc atgcccccgt gaacctggat catccggtcg
     2101 acgacccggt tcaccatctc cgacgcgtag tacttcacga tcgaggattc ctggatgacc
     2161 ggatcgcccg cgtcgtactt ccacgcacat tcataggtca tcaacttcga ggctttgagc
     2221 tctatgaacg actccacgat gtgaccctga acgtattggt tcttaccgat cggtttgtcg
     2281 aacgcgacgc gctggttcgc gtaatcgatc cccaaagaca ggacgtagtc ggcgatccca
     2341 ttgcacatcg aagcgatcga gaggcgtccc attgccagga actgcatggc cgagtagaag
     2401 cccatcccca ccttgtccgg gccaccgagg atctgctctt cgctgacccg gcagtccttg
     2461 aagatgagtt cgaactggcc ctcaccctcc accatccctt tctgggcacg gccgacctcg
     2521 aaccccggcg tgcccttctc gacgatgaac gccgagatcc cgccctgggc tttcttggcg
     2581 cgatcgttgg cggcgaacac gagtgcgaag tccgcgtgct tgccgttcgt gatgaagtgc
     2641 ttggtgccgt tcaagaccca ctgatcgcca tccttcgtcg ccgtggtctt gatgttctgc
     2701 gcgtccgagc cggcgtcggg ttccgtcaag cagaagcaca tcgacttctc tgcagttacc
     2761 agaggaacga ggtactcctt cttctgttcg gccgtcccat tgaggagcaa acccgtgggt
     2821 ccttcgggtc catacgtggt gaacgccgcc aaccggcacc ctgtgcgagc cgcggcttcg
     2881 cgtagtagca ccatcccgag ctgcgggaga ccgctgccgc cgacctcctc agggaaatcg
     2941 gctgcgtaga agccaagctc ggccgagcgc ctgcggatcc gatctctcag ctcgagcgga
     3001 acgtcgtcca cttcgggatc gaggtgctcc tcttccaagg gacgaagttc gcgatctatg
     3061 aagcccgtca ggttcttctg cagttccgaa tattcggccg ggatggtgaa ttccacggtg
     3121 tctccttgct tggttggagc caaaacccta tagcgggccg gaaatatccc cctttctcat
     3181 cgtcatctca tgaaaagggg aaagactctt gctcgcagcg gctatcgaaa cgagcaaaca
     3241 caatgacaac ggtcttcgtt ctcgaagatc atcaactggt tcgcgaggcg atcgccgact
     3301 acctctcctc tcacgggatg gagatagtcg gccaagcggc cacggcaggc gaaggcctga
     3361 ccgggatcac aacgacgcaa cccgatgtcg cgttgatcga cgtcgggttg cccgacggca
     3421 acggtatgga agtatgccgc gaggtggcat cacgccaccc cgacgtcagg tgtttgatga
     3481 tcaccggctt gaccgacgat tcgttggtgg ccgaagccgt actcgccggt gcctacggct
     3541 tcgtcacgaa gaccatcgca ctcgacgatc tccttggtgc ggtcgagaag gtcgccgagg
     3601 gtgtcccgat ggtcgatcct tccgccgcgt acagcctcat cgagaatctc cgcagccggc
     3661 cgagtgatga aggggacgag gcggagctga cccgccagga gagccgcacc ctcgatctga
     3721 tcgcggaggg acttaccaat cgtgagatcg ccgagcgact ccagctggcc gagcagaccg
     3781 tcaagaacta cgtatcgaac atcctcacga agctgggatt cgagcgcaga acccaggccg
     3841 cgctgtatgc agcacgaaag cgcgacctga actaggcggc gatcgcggcg tcgagctttc
     3901 ggaggatcga cttctcaagc gacaggatgc ccttgcgacg gtaaagagct acaacctctt
     3961 cgaggtacgg cagcgccgct cggttccgac cggcgatgag aagaacgtcc gccacaccga
     4021 gaagcgagtg cgtgcgcgtc aatatgtcgt cgctgctcgc gaccggttcc aaagcagcca
     4081 gggccaaggc cttggcctcg tctatgtctc ccgcgcgggc ggtcagcttc gctcgcgtcg
     4141 gcccccactc gacgatccct aacgagtcgc cgttccaggc cgcctcactg gtccacgtcc
     4201 attccagggc ttcgtcgtac cgctcttgcg tgtacaacac acgagagaga aacgctgccg
     4261 agcttgccag cgtggccttc tcttgcatag cctcgagctt cgcgatgccg gctctgaact
     4321 ctgcctcggc ggcggcgtga tcctcggcca gaacctccac catcccggct tcgtgagagc
     4381 tggcggccag cgcgaagttg tgacccagat cctgcaacag ggaccgtcct tcttcgatca
     4441 ggccacgcgc ggtggggaac tcctcctgca tcgctttaag tcctgcgagg cgaagtagag
     4501 acttcgcctt gaccaagagc gaggtctcgg ggagggcgat gatctcctca caccggatga
     4561 tcgcctccgg cacaggctga gggccgagaa ggagcaccgc cgtcatcgaa cccagcaccc
     4621 ttgcgatgtc gcatggagac tccgcaactc gggcgcagat caaagcccgc tcgagggccg
     4681 cttcggtggc gctcagctca tatcgatccc agtgaaggtt cgccttctgg tactcggctc
     4741 ggcccagcca cagccgatcg ccgttcgtct cgaagatgcg agccaactga tcgagcgcgg
     4801 gaagcgcgtc ctccgaccca tcgtccgagc ccaggtagtc gacgatggct cgcttctgaa
     4861 cgaaagcgcg ggcccacaga tcactacc
//
LOCUS       DATMED010000224         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5172272, whole genome shotgun sequence.
ACCESSION   DATMED010000224 DATMED010000000
VERSION     DATMED010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5172272"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VNP73_05685"
     CDS             complement(<1..802)
                     /locus_tag="VNP73_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWL65449.1"
                     /translation="MADDSNSTFDVVILGGGNAGYSAAFRAVKLGLSVAIVEKDKVGG
                     TCLHRGCIPTKALLHAAEFVDEIRGAGEFGILVQEPQVDWSKVLSFKDNVVGRMYQGL
                     SGLVKKKKIELLEGDGRLIDAKTVSVKTADGERAVSAEKGVILAPGSFPRDLPFIEAD
                     GDRVHNSNHGLVAEDIPTSVVVVGGNYIGLEFASVYRSFGAEVTVVEMLPKIAPAEDD
                     DISDALLKLLQRRGIKFHLGISVTGAKPSDGGIEVEIEKEGKTEVLSAD"
     gene            complement(852..1100)
                     /locus_tag="VNP73_05690"
     CDS             complement(852..1100)
                     /locus_tag="VNP73_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWL65450.1"
                     /translation="MRPAAAAIFIIGLAFVVSLLIAGDRANGTFDVVIVALIVGVAWL
                     GWSAMRRFEKIEPSRCPHCDGLVSAGDSVCKHCGEAIS"
     gene            complement(1103..1852)
                     /locus_tag="VNP73_05695"
     CDS             complement(1103..1852)
                     /locus_tag="VNP73_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL65451.1"
                     /translation="MVEERALPVTCDGLRLSCGSAVPETASGIAVLLHGIPSVNPPDP
                     NDTGYPGLARRFADEGWASVWADMRAVRDSEGFFSIEGWVRDAQAVVAVALSLPVAAE
                     LPLALIGSSAGGAVAAEAVRRGSPVDALVLLGAPASWVSFADEPLIGLKRITEEAGMT
                     VSPDVVEDPVAWALEFEGVTTETSIAGVDIPTLVVHGTLDDVVPVDHAIRIADRAPNA
                     ELLLIEGGEHQLRRDQRAVEHVLAWLQKTLA"
     gene            complement(1855..2235)
                     /locus_tag="VNP73_05700"
     CDS             complement(1855..2235)
                     /locus_tag="VNP73_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWL65452.1"
                     /translation="MRILLLSLALVAGLSACSSDGGAEIVMTDTRRYEPATLTVAVGE
                     TVTWRNESREAHSVTAVEASLPDGADYFSSGGYGSEDAARDHLENLMTADETYSVEFA
                     RAGTYEYFCIPHEDQGMKGRIIVE"
     gene            complement(2238..>2807)
                     /locus_tag="VNP73_05705"
     CDS             complement(2238..>2807)
                     /locus_tag="VNP73_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWL65453.1"
                     /translation="LAAETGAALNGTALFAMGAAIAVVGLFVLENILEKRRSGTQGLL
                     LAYLISSGIGLHNLGEGLAVGAALAAGEFALGTFLIVGFALHNTTEGLAIVAPLGAEE
                     RRPSLWHFVGLGAVAGVPTIAGALLGGYAFTPGWATFAFGLAAGAIAQVVWKIGRGLP
                     EQGRLSTAYGAAGFLLGLAVMYVTGLFTA"
BASE COUNT          553 a          963 c          832 g          459 t
ORIGIN      
        1 gatccgccga caggacctcg gtcttgccct ccttctcgat ctcgacctcg atgccgccgt
       61 cgctgggctt cgccccggta acactgatgc cgaggtggaa cttgatgccg cggcgctgaa
      121 gcagcttgag cagcgcgtcc gagatgtcgt cgtcctcggc cggcgcgatc ttcggcagca
      181 tctcgacgac cgtcacttcg gcgccgaacg agcgatagac gctggcgaac tcgagcccga
      241 tgtaattccc gcccaccacg accaccgacg tggggatgtc ctcggcaacc aatccgtggt
      301 tcgagttgtg cacgcggtcg ccgtcggctt cgatgaaggg gaggtcgcgc gggaacgagc
      361 cgggggccag gatgacgccc ttctccgcgc ttacggctcg ctcgccgtcg gcggtcttga
      421 ccgacacggt cttcgcatcg atcagacgcc cgtcgccctc gagcaactcg atcttcttct
      481 tcttaacgag ccccgacagc ccctgataca tgcggccgac gacgttgtct ttgaacgaaa
      541 ggaccttgct ccagtcaacc tgtggttcct gaacgaggat gccgaactcg ccggcacctc
      601 tgatctcgtc cacgaactcg gccgcgtgaa ggagagcctt agtcgggatg cacccgcggt
      661 gcagacaggt gccgcccacc ttgtccttct cgacgatcgc aaccgacaga cccagcttca
      721 ccgcccggaa ggccgcggag taaccggcgt tgccgccacc gaggatgact acatcgaaag
      781 tcgagttgga gtcgtctgcc acaggagcct ccgcactgag tcgctttttc gcgacgatac
      841 attgcgtggg actaggagat cgcctcgccg cagtgcttgc agaccgagtc tccggcagag
      901 acgagcccgt cgcaatgggg gcaacgcgac ggttcgatct tctcgaacct cctcatcgcg
      961 gaccatccca gccaggccac tcccacgatc agagcgacga tcaccacatc gaacgtgccg
     1021 ttggctctat cgcctgcgat caggagactc actacgaaag ccagaccgat gatgaagatc
     1081 gccgcggccg cgggtcgcat ggctaagcca gtgtcttctg caaccaggcg agaacatgct
     1141 cgactgcgcg ttggtcccgc cgaagttgat gctctccgcc ctcgatgaga aggagctccg
     1201 cgttgggggc acgatccgcg atccgaatcg cgtgatcgac cggcactacg tcatcgagtg
     1261 ttccgtgcac gacgagggtg gggatgtcca cgccggcgat cgacgtctcg gtcgttacgc
     1321 cctcgaactc cagtgcccac gcgacgggat cctcgaccac gtccggtgac acggtcatcc
     1381 ccgcttcctc cgtgatccgc ttgagtccga tcaacggctc gtccgcgaac gagacccacg
     1441 aagcaggtgc gcccagaaga acgagtgcat cgacgggact gccgcgtcga acggcttccg
     1501 cagcgactgc accgccggcc gacgacccga taagagcgag aggcagctcc gcggcaaccg
     1561 gaagcgaaag agcgacggcg acaacagcct gcgcgtcgcg cacccaaccc tcgatggaga
     1621 agaacccttc cgaatcacgg accgcacgca tgtcggccca caccgaagcc caaccttcgt
     1681 ccgcgaagcg gcgcgccagt cccggatatc cggtgtcgtt gggatcgggc gggttgaccg
     1741 aagggatgcc gtgcaggagc accgcgatcc cggaagcggt ttcgggcacg gccgatccac
     1801 acgagagtcg cagtccgtcg catgtaacgg ggagtgctcg ttcctcaacc atcactactc
     1861 aacgatgatc ctgcccttca tcccctggtc ctcgtggggg atacagaagt attcgtacgt
     1921 gccggcacgg gcgaactcta ccgagtaggt ctcgtctgcc gtcatcagat tctcgagatg
     1981 gtcgcgcgcg gcatcctccg atccgtatcc accggacgag aagtaatccg ctccgtcggg
     2041 aaggctcgcc tcgacggcgg tgacgctatg agcctcacga ctttcattgc gccacgtcac
     2101 cgtctcaccg accgcgaccg tcagcgtcgc aggctcgtaa cgtcgcgtgt cggtcatcac
     2161 gatctcggcc cccccatcgg agctacatgc agatagcccg gcaacgagcg cgagtgacaa
     2221 cagaaggatg cgcatggcta agccgtgaac aaccccgtga cgtacatgac ggcaagcccc
     2281 agcagaaagc ccgcggctcc gtacgccgtc gacaaccggc cctgctccgg cagccctcgc
     2341 ccgatcttcc atacgacctg cgcgatggcg cccgcggcaa gtccgaatgc gaacgtcgcc
     2401 cagcccggag tgaacgcata accgcccaat aaggctccgg cgatcgtcgg aacgcctgcg
     2461 acggctccca agccgacgaa gtgccacagg gaggggcgcc gctcctcggc ccccagcgga
     2521 gccacgatgg ccagcccctc ggtggtgttg tgcaaggcga acccgacgat caaaaacgtg
     2581 cccaacgcga actcgcccgc ggcaagagcc gctccaaccg cgagtccctc accgagattg
     2641 tgaagaccga taccggaaga gatgagatac gcgaggagca atccctgcgt tcccgaacgt
     2701 cgcttctcga ggatgttctc cagaacgaac agaccgacta cggctatagc ggcccccatc
     2761 gcgaacaatg ctgtgccgtt gagggcagca cccgtttcgg ccgccag
//
LOCUS       DATMED010000225         3770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_548616, whole genome shotgun sequence.
ACCESSION   DATMED010000225 DATMED010000000
VERSION     DATMED010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548616"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1360
                     /locus_tag="VNP73_05710"
     CDS             110..1360
                     /locus_tag="VNP73_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWL65454.1"
                     /translation="MRVFIPGIDGYLGWSLAQHLAARGHEIAGADLFLRRKWVEEMGS
                     WSAIPISPFPERLDAFQERFGRRLTFWEGDLRDYNFVEEIFRDFEPEAIVHLGECPSA
                     PYSMMDRDHAVFVQTSNIVTTFNLLYAIRDVRPDAHLLKLGTMGEYGTPNIDIPEGFF
                     EVEYNGRSDRLPFPRQAGSWYHWSKVHGSNNVMFACKIWGLRATDVMQGVVFGTRIDG
                     QPDDERLRTRLDFDQAFGTAINRFCCEAVVGHPLTPFGKGRQKRGFLPLRDSMQCLRL
                     ALETPPRAAEYRVFNQFDEVYDITELALKVQLVAGGCGLAVEVRHLENPRKELEEHHY
                     NPEHQRLLDLGYTPAHDVEGEIRLMLEDLLQHRDRIEAKIDVLIPDVRWDGTRQKVDF
                     VADDLDRELREVAGGRAAPTPTGL"
     gene            1395..2957
                     /locus_tag="VNP73_05715"
     CDS             1395..2957
                     /locus_tag="VNP73_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWL65455.1"
                     /translation="MRITFLGHAGFCVETSRAIVITDPWLSPSGAFDSAWFQYPRNHH
                     LAAYVQDRLADARKERFVYLSHEHKDHFDPAFLESLRCRDFTVVIADFRRAYLKSAFA
                     EYHCKGVVVCSHEQEVEIPGGKLKLYLDDSELNRDSAILIEAEGYTFLNLNDCRIFDA
                     LPTIAREQGPIDVFTCQFSGAGWHPTCYEYPRKTYEAIARKKMIAKFKSVAQAIRSVA
                     PRVFVPSAGPPCFLDPALSRLNFEAVNIFPRAPKLLQFLQPRLRAVDTSLLELMPGDV
                     LDVDTCTIAYETKDRVSEETFEPYIRAYMASYEGFFLERQRQYQDRDGGMELMGRLGE
                     ALQEKLGLLTLHDRVQTPVYFCLDDLPDQMLRVDFPAKRVDFASRIEDDNHYSITAPS
                     WEVARVLDAKITWDDFSLTFRMRLNREPDVYQTVVQGFLRLEPEDMNWFCSKLLNIEN
                     NQERIIVEAGGKRYAVDRYCPHQGGDLTQAWIEDERFLTCARHQWQFDLEQDGNARRN
                     EGSIHAVCLDDD"
     gene            3007..>3770
                     /locus_tag="VNP73_05720"
     CDS             3007..>3770
                     /locus_tag="VNP73_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65456.1"
                     /translation="MGLAIALGLAVTSSAWPAPPEDSQLEELWSTYPLDPVTDDERKR
                     VLRTPDAQARPVPDIGPTPDVSASLDEPASLGPLLFAGLGLVVVGSTLALFASWTRSR
                     NRPSSSRVRTGQREPDRELSRARNGGRSRGLARRSSEVGLEDVTGSAVGTSGKDIDRI
                     PDQIVQEAEEQRALSRADLERLLALVVEVADDRLTPPNPEIERLLGRIVHHAEAQSAF
                     SNGDGMSRGANGADGGSAAVGSWAAPGGLSWSGPSM"
BASE COUNT          677 a         1197 c         1198 g          698 t
ORIGIN      
        1 gcggcatgcg gtctgcggca tgcgggtttc gcgggatccc tgactcaggg tcaggggcgg
       61 ttgcgcgccg ccccgggcgt caccttgggg gagagagggg aactgggata tgcgggtctt
      121 cattcctggc atcgatggat acttgggttg gtctctcgcg cagcatttgg cagcgcgcgg
      181 gcatgagatc gccggagccg atctcttcct ccggcgcaaa tgggtggagg agatggggtc
      241 gtggagcgcg atcccgatct ccccgttccc cgaacggctc gacgctttcc aggagcggtt
      301 cggcaggcgg ctgactttct gggaggggga tctccgcgac tacaacttcg tcgaggagat
      361 cttccgggat ttcgagccgg aggcgatcgt ccacttgggc gagtgcccct cggcgccgta
      421 ctcgatgatg gatcgcgatc acgccgtgtt cgtgcagacg agcaacatcg tcacgacctt
      481 caacctgctc tacgcgatcc gcgatgtgag gccggatgcc catctcctga agctcggcac
      541 gatgggtgag tatgggactc cgaacatcga cattccggag gggttcttcg aggtcgagta
      601 caacggaagg tcggatcggc ttccgttccc gcgtcaggcg ggttcgtggt accactggag
      661 caaggttcac ggatcgaaca acgttatgtt cgcgtgcaag atctggggcc ttcgcgcgac
      721 ggacgtcatg caaggagtcg tgttcgggac gcggatcgac gggcaaccgg acgacgagcg
      781 cctgcgaacg cgtctcgatt tcgaccaggc gttcggaact gcgatcaaca ggttctgctg
      841 cgaagcggtc gtgggccatc cgctcacacc cttcggcaag gggcgccaga agcggggctt
      901 tctgccgctg cgcgattcga tgcagtgcct gaggctcgcc ctcgagaccc cgccccgagc
      961 cgcggagtac cgggtgttca accagttcga cgaggtttac gacatcacgg agctcgcgct
     1021 caaggtgcag ctcgtcgccg gcgggtgcgg attggccgtc gaggtccgac atctcgagaa
     1081 cccgcggaag gagctcgagg agcatcacta caaccccgag caccaacgcc tcctcgacct
     1141 cggatacacg ccggcacacg acgtcgaggg ggagatccga ctcatgctcg aggatctgct
     1201 ccagcaccgc gatcgcatcg aggcgaagat cgacgtcctg atcccagacg ttcggtggga
     1261 cggaacgcgg cagaaggtgg acttcgtcgc tgacgatctc gaccgagagc tcagagaggt
     1321 ggccgggggt cgagccgctc cgacaccgac cggcctatga tcccgcttct cgccgccccg
     1381 tctccgtggc cgcgatgaga atcacgttcc tcggtcacgc cggtttctgc gtcgaaacct
     1441 cgcgggcgat tgtcatcaca gacccctggc tctcgccaag cggcgccttt gattccgcgt
     1501 ggttccagta cccgcggaat catcacctcg ctgcctacgt ccaggacagg ctcgccgatg
     1561 cgcgaaagga acggttcgtt taccttagcc acgagcacaa ggatcacttc gatccggcct
     1621 tcctcgagag cctgcgctgc cgcgacttca cggttgtcat cgccgacttc cggcgcgcct
     1681 atctgaagtc ggcgttcgcc gagtaccact gcaagggcgt tgtcgtgtgt tcccacgagc
     1741 aagaggtcga gatcccgggg ggaaagctca agctctacct ggacgactcc gaactgaacc
     1801 gcgactcggc gatcctgatc gaggccgagg gctatacgtt cctgaatctc aacgactgca
     1861 ggatcttcga cgcgcttcca acgatcgcgc gcgagcaagg tccgatcgac gtctttacct
     1921 gccagttctc gggcgcgggc tggcacccca cctgctacga gtacccgcgg aaaacgtacg
     1981 aggcgattgc cagaaaaaag atgatcgcca agttcaaaag cgtcgcccag gcgatcaggt
     2041 cggtcgctcc tcgggtgttc gtgccgtccg cgggaccgcc ttgcttcctt gacccagcgc
     2101 tttctcgcct caacttcgag gccgtgaaca tcttcccgcg cgcaccgaag ctcctccagt
     2161 tcctccagcc ccgactccgg gccgtcgaca cgtcgctcct cgagctgatg ccgggcgacg
     2221 tcctcgacgt cgacacgtgc acaatcgcgt acgagacgaa ggaccgcgtc agcgaggaga
     2281 ccttcgagcc atacatccgc gcgtacatgg cgtcgtatga gggattcttc ctggagcggc
     2341 agcggcagta tcaggatcga gacggcggca tggagctcat gggccggctc ggggaagcgt
     2401 tgcaggagaa gctgggcctt cttaccctgc acgatcgagt tcagacgccc gtctacttct
     2461 gtctcgacga tcttcccgat cagatgctcc gtgtcgactt ccctgcgaag cgtgtcgatt
     2521 tcgcgagccg aatcgaggac gataatcact actcgatcac ggctccgtcc tgggaagtcg
     2581 cccgcgtgct cgacgcgaag atcacctggg atgacttctc attgacgttt cggatgcggc
     2641 tcaaccggga gccagacgtc tatcagaccg tcgtccaggg cttcctccga cttgagcccg
     2701 aggacatgaa ttggttctgc tcgaagctgc tcaacatcga aaacaatcaa gagcggatca
     2761 tcgtcgaagc cggcggaaag aggtacgcgg tcgatcggta ttgcccccat cagggagggg
     2821 atctgactca ggcatggatc gaggatgagc gtttcctcac ttgtgcccgg catcaatggc
     2881 agttcgatct ggagcaggac gggaacgcac gcaggaacga agggagtatt cacgcggtct
     2941 gtctcgacga cgactgaggc cgttccctac tgccggagcc atgtgccgcg gccgtttctc
     3001 ctctgcgtgg gcctagcgat cgccctcggg ctcgccgtca cctcaagcgc atggccggcg
     3061 ccgcccgagg acagtcaact cgaagagctc tggtcgacct accctctcga cccggtcacg
     3121 gacgacgaga ggaaacgggt cttacgaacg ccggatgccc aagctcgtcc tgtccccgac
     3181 atcgggccca cacccgacgt ttccgcatcc cttgacgagc ctgcgtccct tgggcctcta
     3241 cttttcgccg gtctcggcct cgtcgtcgtg gggagcacgc tcgcgctctt cgcgagctgg
     3301 acgcgttccc gtaatcgccc gtcctcctcc agagtgagga cgggccaacg cgagccggac
     3361 cgcgaacttt cgcgtgcccg aaacggaggt cgaagccgcg gtctcgctcg gcgctcgtcg
     3421 gaagtcgggc tcgaagatgt gaccggcagc gcggtgggca cgtccgggaa ggacatcgac
     3481 cggatccccg accaaatcgt gcaggaagcg gaggaacaac gcgccttgtc aagggccgac
     3541 cttgaacggt tactcgccct cgtcgtggag gtggcggacg atcggctcac cccgcccaat
     3601 cccgagatcg agcgattact cggtcgcatc gtgcaccacg ccgaggcgca atccgcgttc
     3661 tcgaacggtg acgggatgag tcgcggcgcc aacggagcgg atgggggctc cgcggcggtg
     3721 ggctcgtggg ccgctcctgg tggcctttcg tggagcggac cgtccatgtt
//
LOCUS       DATMED010000226        13736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_929499, whole genome shotgun sequence.
ACCESSION   DATMED010000226 DATMED010000000
VERSION     DATMED010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929499"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..413)
                     /locus_tag="VNP73_05725"
     CDS             complement(21..413)
                     /locus_tag="VNP73_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWL65457.1"
                     /translation="MVTGRMKLEQLGSFIREQRRSARLSLRKLSDLAGISNPYLSQIE
                     RGLRKPSAEILQSIAKALRISAETLYVQAGILEENRDHDLVAEILKDSLITERQKQAL
                     VDHYRSYVESNTPPHEKAASVQDISEAK"
     gene            635..1429
                     /locus_tag="VNP73_05730"
     CDS             635..1429
                     /locus_tag="VNP73_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65458.1"
                     /translation="MKPGNRLTPVIAVGLAAIVGASLVALSLIAGEITLPSPIGAPVI
                     PNRRDDSGPVAPVQVPGSRAENETGGRRETQVAASEQPDGDVVQGTRIADAGENPIPV
                     PIATTGAGAPDDPPQGPEEPEGPDDQPTVKPPMGGDDGTGRTPTGRPPSDPDDDDDDD
                     DDDDDNRDDDHGRDKDDEDSDDDEWVPPGQAKKGNDAPGHSGDKTPPGHAKKDNDDAR
                     RDDEDEDDGDSDDDDDSDDSTDSHSHTNGNGHSDSRGKGHDKSKHD"
     gene            complement(1440..1976)
                     /locus_tag="VNP73_05735"
     CDS             complement(1440..1976)
                     /locus_tag="VNP73_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65459.1"
                     /translation="MPEHPERRKTDILFKDNYMEGLADRTIDELKAMRDECAEAENEI
                     SFERRLCQARLDILSAELDHREGRAGDVISRLPEILAQEGGGNSTLPKRAPDMSAPEG
                     ADKPRRRVEEIVGEQTLARLADLDVEEIRTIIDKLAEHERNLSRDRRHLHEMLDQIQA
                     EMVGRYTRGEANPDAILG"
     gene            2000..2809
                     /locus_tag="VNP73_05740"
     CDS             2000..2809
                     /locus_tag="VNP73_05740"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HWL65460.1"
                     /translation="MEPLIITVAAVGAELSPEEQPNLPITPDELARDAAACREAGACI
                     YHLHVRDSAGTPTMDVDVFRTARDAIVEATDLIVQFTTGGAVSDAPEDRIAPLELQPE
                     MASLTTGTVNFGDEVFFNPLPLVREFHQRMVDMGVVPEFEIFEPGMIATAERVYEEHG
                     SSHHKHFDFVLGVPGAMPAWPDAVEWLSSKLSDGATWSATGIGRHNFPVAATSVAVGG
                     HVRTGFEDVRYIEPGKLVRSNRELVERVVAIGRDGGREIATPAQAREVLGL"
     gene            complement(2806..3864)
                     /locus_tag="VNP73_05745"
     CDS             complement(2806..3864)
                     /locus_tag="VNP73_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWL65461.1"
                     /translation="MPEPRPAKRRREFPFNGLARVALIAILLVVNGASVFTGRAAGAD
                     STSELEERMDSIQARLDQTTARIEDLRTQKEHVEGRIAGIKTRVGALSDREARLESTA
                     VNRAEELYKSGTSTMVATLLDASSIHQVMDRAEVMSQVSMDDSNVFISLSRAQAELKQ
                     LTADLKIERGSLATAESSLADESKRLQTQFADIKSEYMALARKTAPATTQVSAAPSAP
                     LLKSTDGMYCPVAGPVSFVDSWGAPRSGHTHQGVDMMAAYGTPIAAVTDGTITYAAYD
                     GSGGNMLWLSGTDGNAYYYMHNQENYVGQGASVSAGEIIGTVGDTGNAAGTPHLHWEY
                     HPGGGAAVNPTPLAASLC"
     gene            4150..4836
                     /locus_tag="VNP73_05750"
     CDS             4150..4836
                     /locus_tag="VNP73_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWL65462.1"
                     /translation="MRGVEDYLEEIKRLEEDQERITATVLARILQVSLPSASEMLKRL
                     AAEGYLSREKGGSILLTPEGRRLAHTMLRRHRLVECLLVDKLGMAWFEVHGEAHKIEH
                     AISDRVEEAMAQSLDYPDYCPHGHPICPVDLRELKRLTELEAGHGGVVAQISEVKEDV
                     LPYLDQIGVRPGAEVKVKDIAPREGPLTVETEEGATALSHDVAAIVRVTPNLSRESAL
                     EGYSVSQERR"
     gene            complement(4833..6044)
                     /locus_tag="VNP73_05755"
     CDS             complement(4833..6044)
                     /locus_tag="VNP73_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL65463.1"
                     /translation="MPSADHLTESTGPIRPDTRGVSAEDFELFRPEHDELRSAVRAYV
                     QKELLPNAEAWEKAEEFPKDTFKRIGDLGFFGLKYPEDVGGSGPDFLADAVVTEEFAG
                     CGSGGVSSCLGAHKDLSTLYVYNFGTPELHKKWLVPALAGEVVGALAVTEPDTGSDVA
                     SIATRAKPDGSDWIINGNKTFITNGAWADFIVVATKTDPDAGHDGLTLFVVDTDTPGF
                     SARKLRMLGWRPGQTGELSFADVRVPKEAMLGEPGSGFKAIMQNFAWERLIMALGAIA
                     GAQRVYEIAKEYALDRKAFGRPVGKFQVWRHRFADIATRIEAGRAMTYNALRLFAAGE
                     NPIREVSMAKLYACELAFHVADECVQVHGGYGYTTEFPAERALRDARLGPIGGGTSQI
                     MKEIIGKTYGL"
     gene            complement(6044..6907)
                     /locus_tag="VNP73_05760"
     CDS             complement(6044..6907)
                     /locus_tag="VNP73_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWL65464.1"
                     /translation="MAVPPVYKFGTEEQRQKYLVPAIKGDLIFCLGITEPNAGSDVQA
                     IETMAKKVDGGWVINGRKIFITNGKRAHAMTLVAKTERDAGHGGHSLFIVDTDSEGYE
                     VARTLDKLGMHSSDTGEISFTDMFVPDDALLGKEGEGFYNISWELQGERLIGAAGSIA
                     GARRAFEGALAYAKERKSFGKPIGQHQAIRHLLADMATEVEAAQQLVYTAAWKVNKGQ
                     YPVREISMAKLITGEVAWRTIDRTMQIFGGHGYSMDLPIQRGWRDSRLIRIGGGTDEV
                     MRDVIGKMEGF"
     gene            complement(6890..7060)
                     /locus_tag="VNP73_05765"
                     /pseudo
     CDS             complement(6890..7060)
                     /locus_tag="VNP73_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            7211..7468
                     /locus_tag="VNP73_05770"
     CDS             7211..7468
                     /locus_tag="VNP73_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65465.1"
                     /translation="MNARGTALVLALFVACGGSSSTPSSGGTSLFGELKTSPAESPAK
                     DRSGGGGYAAPDFTVTTFEGEEFRLAVHKGTPVVLNFWESW"
     gene            7505..7759
                     /locus_tag="VNP73_05775"
     CDS             7505..7759
                     /locus_tag="VNP73_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65466.1"
                     /translation="MAEEYEGEVVFVGVSNNDTVADGKGYVTEFDVPYAMAHAPEVWD
                     LYDVPYQPVTIVLDAGGKEVERFDGPVETEPLRTVIEKTL"
     gene            complement(7761..8027)
                     /locus_tag="VNP73_05780"
     CDS             complement(7761..8027)
                     /locus_tag="VNP73_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HWL65467.1"
                     /translation="MNEFLYGITAMGCLVAALFFAKFWRQSHDRLFMIFGIAFLTFAI
                     NRVLLALVPATYEATTYIYGLRLAAFVLIIVAIVDKNLAGRRDR"
     gene            complement(8032..8298)
                     /locus_tag="VNP73_05785"
     CDS             complement(8032..8298)
                     /locus_tag="VNP73_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HWL65468.1"
                     /translation="MAETVYLLCAVTSIICAVLLIRAYLHSRLRLLFWSSLCFLGFAL
                     NNGILFFDKVVVPHVDLSLLRHTPAVIGIACLLYGFIWESDDPS"
     gene            complement(8301..8690)
                     /locus_tag="VNP73_05790"
     CDS             complement(8301..8690)
                     /locus_tag="VNP73_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65469.1"
                     /translation="MGQKDVSKNVRDFLARFIDSVELLEVLLLARAAPEKAWSTKDVA
                     RALTLSDESADLRLRQLAGRGLLAADIKGGTFTYSPESADMDKTIDDVAETYAKRRTT
                     VIGLIFSGPSPSVRGFADAFRLRDEDE"
     gene            8736..9197
                     /locus_tag="VNP73_05795"
     CDS             8736..9197
                     /locus_tag="VNP73_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HWL65470.1"
                     /translation="MHKGLERVSYLLGTWRGEGKGRYPTIDDFAYGEEVKFWHDGRPL
                     LFYAQRTWNLETDGPMHSETGFWRPQRDGSIEIVLAHTFGCVEIQEGTVEGNRIGTKS
                     KGVVSTSSAKDVVALARHYSVDGDTLSYELEMQFDDNELQNHLSGTLTRQS"
     gene            complement(9194..9583)
                     /locus_tag="VNP73_05800"
     CDS             complement(9194..9583)
                     /locus_tag="VNP73_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65471.1"
                     /translation="MNIFDEEFDERELARALGFMRIFVGGVLFLMPHRSARGWTGEEA
                     DEVTTNLAIRGMGARDIAIGIGILTALEKGGSVRGWIEAGVVSDAGDALATLANWRDL
                     RKGRGLFWLAMEAGTAYFGSLVAQELD"
     gene            complement(9580..10092)
                     /locus_tag="VNP73_05805"
     CDS             complement(9580..10092)
                     /locus_tag="VNP73_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWL65472.1"
                     /translation="MPKPFKFCPACASEVDVGDEEERICGSCGRNWYRNSSPSVGAAI
                     VKDGRALVTVRGSDPYKGKFDVPGGFLQLGEDVIDALRRELREELDIEVDVAQSDFVH
                     MVTHTYGEEDDWVLAIGFVARLTSGTPTPASDVADARWVSLEELDGLEFAWEHDRELA
                     RKALTMEVEG"
     gene            complement(10095..10655)
                     /locus_tag="VNP73_05810"
     CDS             complement(10095..10655)
                     /locus_tag="VNP73_05810"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWL65473.1"
                     /translation="MPKIYTKKGDDGTTGLLYGGRVTKDDIRTEVYGTLDETVSALGL
                     ARAAGLVDRIESIVVRVQREMFVAGAELATHADQHDKLKEGISKVTASMTEEAERDID
                     ALLEEHPLPQEFLLPGETSGSAALDLARSIVRRAERLAVAMQRADLIPNPEVLRYVNR
                     VSDLLYACARYEEAERGVAAKPSRER"
     gene            complement(10722..11123)
                     /locus_tag="VNP73_05815"
     CDS             complement(10722..11123)
                     /locus_tag="VNP73_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65474.1"
                     /translation="MRRTAVPAAASLSLFLLFFLMGGTALAGPSGSQETANEVDCNED
                     SSTSAAGVAYVYAGTNGAEACADNTSAFPIDGSATVDAEDGYAAIDGDNSNAEPANGY
                     ARVDGTGVHCGNETNQDSSADQSGNTATDCG"
     gene            complement(11244..12092)
                     /locus_tag="VNP73_05820"
     CDS             complement(11244..12092)
                     /locus_tag="VNP73_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65475.1"
                     /translation="MALPPPTRYQKVAYACGALLLAAGAFHFIVFLIKGGPWEGPISW
                     RKPVLFGFSFGITVVTLTWVMSFLKLKPLSGWIAAGVMSVSALLEVFLVSMQKWRGVE
                     SHFNDKTTFDSSVFGIMGLMVGVMIFVIVYLTVRAFIRLEAPPSLALGIRLGFLILLL
                     SQATGIMMIAVYGNVWGDAGAMKTPHALTMHGIQVLPALAVLLGRADTTEERRVRLVR
                     MAAMGYAALAAATLIQTYSGRATFDVLPVSVPIAVLGFTLLGAVGLLALLGLRPARNG
                     RQVNPT"
     gene            complement(12097..13236)
                     /locus_tag="VNP73_05825"
     CDS             complement(12097..13236)
                     /locus_tag="VNP73_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016457186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWL65476.1"
                     /translation="MGFPTEEQISQVLGEIQDPELHRGLNDLNMVRNIAIEGSDVTVL
                     VALTIPGCPLKDYFHTVIPAKLKESFSEINAVRVELTSMTEEERESLIGGLRKDTPAP
                     FARSDSPTQVLAIGSGKGGVGKSTTTVNIAVALAQLGHTVGLLDADVWGFSVPRMFDL
                     HERPTVVGDMVMAPEKFGVKVISIGLFTAEDNPVVWRGPMLHKALQQFLTDVHWDEPD
                     YLLVDLPPGTGDVSISIAQFLPGAHMVIVTTPQPVAEKVAQRAGFMAEKTGLSVLGVI
                     ENMSYFRGDDDKQYRIFGEGGGASLANRLGVPVLGEIPLDPQAREFADNGTPVVLQEP
                     GSEVAQQLMATAKEIVNLIPPKPRPAKRISLPLLPNINAGHSHSH"
     gene            13329..>13736
                     /locus_tag="VNP73_05830"
     CDS             13329..>13736
                     /locus_tag="VNP73_05830"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWL65477.1"
                     /translation="MKRSGVLTLLVSVVLMSMSVAAGAAGQPPESREDRYTVKAGRQL
                     VVSAADGLLANDSDPEGDKLTAVLSHQRPPGKLSLRPDGSFRYTPISSGGNDFFSYTA
                     FDGQSHSPPTFVIIRVDAAPITQPDSYGVIAPAP"
BASE COUNT         2641 a         4319 c         4267 g         2509 t
ORIGIN      
        1 cgtcccgtca agtctgaaca tcacttcgcc tcgcttatgt cttggaccga agccgctttc
       61 tcgtgcggag gcgtgttgct ctcgacgtag gagcggtagt ggtccactag agcctgcttc
      121 tggcgctcgg ttatgaggct gtccttgagg atctcggcaa cgagatcgtg atcgcggttc
      181 tcctcgagga tcccggcttg gacgtacagg gtctcggccg agatacggag ggccttggcg
      241 atcgattgca ggatctcggc cgacggcttc ctcaagcccc gttcgatctg ggagaggtag
      301 gggttggata taccggccag gtcggagagc ttcctcagcg acaggcgggc agagcgacgc
      361 tgctccctga taaaggagcc cagctgctcg agcttcatcc gtcctgtgac catctaccct
      421 ccatctttcc ccgagtggtt gctttataac ccacttgtta aacttttgca agcactttga
      481 gattgcctga tatacgtatg aggcggactt attagcgaga caagacctgc cgatatctct
      541 agaaagcagg agaacgccgg gtgcatagtt ctaagtgcat ccggggaggt aggtgagtca
      601 tgcgcgtaga aagcctcagg ccaagggggg gcggatgaaa cctggcaatc gactcacgcc
      661 cgttatcgct gtcggattgg ctgcgatagt cggagcttcc ctcgtcgcgc tctcacttat
      721 cgccggagag ataacccttc cgtccccgat cggcgctccg gtcatcccga accgccgcga
      781 cgacagcggg ccggttgctc ccgtgcaggt acccggcagt agggctgaga acgagacggg
      841 cggccgccgc gagactcagg tcgcagccag tgagcaacct gacggcgacg tcgttcaagg
      901 aacgcgaata gcggatgcgg gcgagaaccc aatccccgtg ccgatcgcaa cgaccggcgc
      961 cggcgcgccc gacgaccccc cgcaggggcc cgaagaacct gaaggtcccg acgaccagcc
     1021 caccgtcaag ccgccgatgg gtggcgacga cggaaccggc cggaccccaa ccggacggcc
     1081 gccgtccgac cccgatgatg acgacgacga tgatgacgac gacgacgaca atcgcgatga
     1141 cgaccacggc agagacaaag acgacgagga ctcagacgac gacgagtggg tacctccggg
     1201 ccaggccaag aaaggcaacg atgcgccggg ccactcgggc gacaagaccc cgccgggtca
     1261 cgcgaagaag gacaacgatg acgcgcgccg ggacgatgaa gatgaggatg acggcgactc
     1321 agacgacgat gacgactcag acgattcgac ggatagccac agtcacacca acggcaatgg
     1381 ccacagcgat agccgcggga agggccacga caagagcaag cacgactagt tagctggcgc
     1441 tagcccagga tcgcgtccgg gttcgcttca cctctcgtgt aacgaccgac catctccgcc
     1501 tggatctgat cgagcatctc gtgaaggtgt ctccgatcgc gcgacaggtt ccgttcgtgc
     1561 tccgcgagct tgtcgatgat ggtcctgatc tcttccacgt cgaggtcggc tagacgagcg
     1621 agcgtctgct cgccgacgat ctcttctacc cgccgcctcg gcttgtccgc gccttcgggg
     1681 gccgacatat cgggtgcccg tttggggagc gtgctgttac cgccgccttc ttgggcaagg
     1741 atctcgggca aacgactgat gacgtcgccg gcccttccct cacggtgatc cagttcggcc
     1801 gagaggatgt cgagccgggc ctggcacagc cggcgctcga aggagatctc gttctcggcc
     1861 tcggcgcact cgtcccgcat ggccttcagc tcgtcgatcg tgcggtcggc gaggccctcc
     1921 atatagttgt ctttgaagag gatgtcggtc ttgcgacgct ccgggtgctc gggcatggag
     1981 cgagcgtacc ctcaacccga tggagcctct gatcatcacg gtcgccgcag tgggggccga
     2041 actgtcacca gaagagcagc cgaaccttcc catcaccccc gacgaactgg cgcgggacgc
     2101 ggccgcgtgc cgtgaggcgg gagcgtgcat ctaccacctg cacgtacggg actccgccgg
     2161 gacgccgacg atggacgtgg acgtcttccg aaccgcccgg gatgcgatcg tcgaggccac
     2221 cgacctgatc gtgcagttca caacgggcgg tgccgttagt gatgcgccgg aggatcggat
     2281 cgcgccgctc gagctacagc cggagatggc cagtctgacc acggggaccg tcaacttcgg
     2341 cgacgaggtg ttcttcaatc cactccccct cgtacgcgag tttcatcaac gcatggtcga
     2401 catgggtgtc gtgccggagt tcgagatctt cgagcccggg atgatcgcca cggccgagcg
     2461 cgtctacgaa gaacatgggt cttcgcatca caaacatttc gatttcgtgc tgggggtgcc
     2521 gggtgcgatg ccggcgtggc ccgatgcggt ggagtggttg tcatcgaaac tttcggacgg
     2581 tgctacgtgg agcgcgacgg ggatcggtag acacaacttc cccgttgcag ctacgtccgt
     2641 ggcggtaggt gggcatgtga ggacgggatt cgaggacgtt cgttacatcg agccggggaa
     2701 gctcgtacgg tcaaaccgag agttggtcga acgcgtagtg gctatcgggc gcgatggggg
     2761 ccgcgagata gcaacacccg cgcaggcgcg agaggttcta gggctctagc agagggacgc
     2821 ggcgaggggg gtcggattta cagcggcccc cccacccggg tggtactccc agtgcaggtg
     2881 tggcgtgccg gccgcgttgc cggtgtcacc gacggttccg atgatctcgc cggcgctcac
     2941 cgatgcacct tggcccacgt agttctcctg gttgtgcatg tagtagtacg cgttgccgtc
     3001 cgtgcccgag agccacagca tgttgccccc gctgccgtcg tacgcggcgt aagtgatcgt
     3061 tccgtcggtc acggcagcga tcggggtgcc gtaggccgcc atcatgtcga cgccctgatg
     3121 cgtatgaccg cttcgaggcg ctccccacga gtcgacgaac gaaacggggc cggccaccgg
     3181 gcagtacatg ccgtcggtgg acttgagcag cggtgcactc ggggccgccg agacctgagt
     3241 ggtcgccggt gccgtcttgc gtgcgagagc catgtactcg gacttgatgt cggcgaattg
     3301 ggtctggaga cgcttggact catcggcgag gctgctctcg gccgtggcca agctgccacg
     3361 ctcgatcttc aggtctgcag tgagttgttt gagctcggcc tgtgcacggg acagtgagat
     3421 gaacacgttc gagtcgtcca tggagacctg cgacatgacc tcggcccggt ccatcacctg
     3481 gtggatgctc gatgcgtcga gcagtgtggc aaccatcgtg gaggtgccgg acttgtagag
     3541 ctcctcggct cgattcacgg cggtcgactc cagcctggcc tcacgatcgg acagtgcacc
     3601 cactctggtc ttgatgccgg ctatacggcc ttcgacgtgt tccttctggg ttcggaggtc
     3661 ttcgatgcgg gccgttgtct ggtccaaacg ggcctggatc gagtccatcc gctcctcgag
     3721 ctcgctcgtc gagtcggcgc cggcagcgcg gccggtgaac acggatgctc cgttaaccac
     3781 gagcaggatg gcgataagcg ctacacgcgc gagaccgttg aacgggaact cgcggcgacg
     3841 cttcgcgggc cgcggttctg gcaccggctt cggttccgga cgagcgggtt ccggcgcgcg
     3901 cggaaatgcc tcgctcacca aagctttggt ggcgagggct aaaacgacga tggccccgag
     3961 acgggccatt cgggcgccgc gcaaccgcac ggcgagggac gctagcacgg gggagttttt
     4021 cgcaacctgg acgggaactc ctgcacacag cttgcacaga tgtcgacgcg tagcgtcatc
     4081 caggcggtgc ttgtgaacaa cgcgccctaa ttacatccct gtaagggagc atgcacttat
     4141 tctcaaagtg tgcgtggtgt ggaggattac ctcgaagaga tcaagcgctt ggaggaggac
     4201 caagagcgca tcacagcgac cgtgctggcc cggatcctcc aggtgtcgct cccgtccgct
     4261 tccgagatgc tgaagcggct ggctgcggag ggatacctgt cacgggagaa gggcgggtcc
     4321 atcctgctca ccccggaggg ccgacggctc gctcacacga tgttgaggcg ccatcgcctc
     4381 gtggagtgcc tattggtcga caagctcggc atggcgtggt tcgaggtcca cggggaggcc
     4441 cacaagatcg agcacgcgat ctcggatcgg gtcgaggaag cgatggcgca atcgctcgac
     4501 tatcccgatt actgccccca tggtcacccg atctgccccg tggacctacg tgaactgaaa
     4561 cggctgacgg aattggaggc cggacacggt ggcgtcgtcg cgcagatctc cgaggtgaag
     4621 gaagacgtgc tgccctatct ggatcagatc ggggtgcggc cgggggccga ggtgaaagtg
     4681 aaagacatcg ctccacgcga agggccgctc actgtagaga ccgaagaagg agcgacggca
     4741 ctgagtcacg acgtagccgc gatcgtgcga gttacgccga acttgtcgcg tgagagcgcg
     4801 ctggagggtt actcagttag ccaagaacgg cgttagaggc cgtaggtctt gccgattatc
     4861 tctttcatga tctgagaggt tccgcccccg atggggccga gccgggcgtc gcgaagagct
     4921 cgctcggcgg ggaattcagt ggtgtagccg tagccgccgt ggacctgcac gcactcgtcg
     4981 gcaacgtgga acgcgagttc gcacgcgtac agcttcgcca tcgagacctc acggatcggg
     5041 ttctcgcctg cggcaaagag cctgagggcg ttgtaggtca tcgctcggcc ggcttcgatg
     5101 cgcgtcgcta tgtcggcgaa gcggtgacgc cacacctgga acttgccgac cggacgaccg
     5161 aacgccttgc ggtcgagcgc atactccttg gcgatctcgt acacgcgttg cgcaccggcg
     5221 atcgcgccga gcgccatgat caaccgctcc cacgcgaagt tctgcatgat cgctttgaac
     5281 cccgagccgg gttcgcccag catcgcctct ttcggcacac gcacgtcggc gaacgaaagt
     5341 tctccggtct gccccggccg ccagccgagc atccgtagct tgcgggccga gaatcccggc
     5401 gtgtcggtat cgacgacgaa caaggtgagg ccatcatgtc ccgcatcggg atcggtcttc
     5461 gttgcgacga ctatgaagtc ggcccacgct ccgttcgtga tgaaggtctt gttgccattg
     5521 atgatccagt cggagccgtc gggcttggca cgggtcgcga tcgaggcaac gtccgagccg
     5581 gtatcgggct cggtcacggc cagagcgccg acgacctcgc ctgccaacgc cggaaccagc
     5641 cacttcttgt gtagctcggg cgtaccgaag ttgtagacgt acagggttga gaggtcttta
     5701 tgtgcaccga gacaggacga cacgccgccg gaaccgcacc ctgcgaactc ctccgtaacg
     5761 acggcgtcgg ccaggaagtc gggtcccgag ccgccgacat cctcgggata cttcagccca
     5821 aagaagccga ggtctccgat ccgcttgaag gtgtccttcg ggaactcctc cgccttttcc
     5881 cacgcttcgg cattcggaag tagctctttc tggacgtagg cacgtaccgc cgagcgcagt
     5941 tcgtcgtgct cgggacggaa cagctcgaag tcctcggccg acacgccgcg ggtatccggc
     6001 cggatcggac ccgtggattc ggtgaggtga tcggccgaag gcactagaag ccctccatct
     6061 ttccgatcac gtcgcgcatg acctcgtcgg tgccgccgcc gatacggatc agacgggagt
     6121 cgcgccaccc gcgctggatc ggcagatcca tggagtagcc atggcccccg aagatctgca
     6181 tcgtgcggtc gatcgtgcgc cacgcgacct cgccggtgat caacttcgcc atcgagatct
     6241 cgcgcaccgg gtactggccc ttgttgacct tccacgccgc ggtgtagacg agttgctgcg
     6301 cggcctcgac ctcggtcgcc atgtcggcga gcaaatggcg gatcgcctgg tgttgaccga
     6361 tcggcttgcc gaatgacttg cgttccttcg cgtatgcaag cgcgccttcg aatgcgcgtc
     6421 gagcaccggc gatcgaaccg gcggcaccga tcaggcgctc accctgtagt tcccacgaga
     6481 tgttgtaaaa gccttcgccc tctttgccga gcagggcgtc gtcgggaacg aacatatcgg
     6541 taaaggagat ctcgccggta tcggacgaat gcatgccaag cttgtcgagc gtgcgcgcca
     6601 cctcgtaccc ctcggagtcc gtatcgacta tgaaaagtga gtgcccaccg tgcccggcgt
     6661 cgcgctcggt cttcgctacc agcgtcatcg cgtgcgcgcg cttgccgttg gtgatgaaga
     6721 tcttccggcc gttgatcacc cagccgccgt cgaccttctt ggccatcgtc tcgatcgcct
     6781 ggacgtcgga gcctgcgttc ggctcggtga tcccgaggca gaagatcagg tcacccttga
     6841 tcgccggaac gaggtacttc tggcgctgct cttcggtccc gaacttgtag accggcggga
     6901 cggccatgtc ggtctgcacc gcgaccgcca tgctgaggcc gcccgagtta caacgaccca
     6961 tctcttcggc catcacgagg gtgcagaggt agtcgcctcc gccgcccccg tacttctcgg
     7021 ggtaggagag accgaggaaa ccggcgtcac ctagcttctt gaagacccag tcggggaagt
     7081 acttctgctc ttcccactcg gccgcgtggg agccagctct ttctcgacga agtcacggat
     7141 ctccttgcgg agaagctcgt gctcttcggt gaagggccag cgcgccatgc cgcatcctag
     7201 acttttgacg atgaacgccc gcggtacagc cctcgtcctg gcgttgttcg tagcttgcgg
     7261 aggttcgtcc tcgacgccct cgtccggcgg aacctccctg ttcggcgagc tgaaaacgtc
     7321 tcccgcagaa tccccagcca aggacagatc cggtggtggt ggctacgcgg cccccgattt
     7381 caccgtcacg accttcgaag gtgaggaatt ccgtctcgcc gttcataaag gcaccccggt
     7441 cgtcctcaac ttctgggagt cgtggtgacc acagtgccag ggcgaacagc cccacctcaa
     7501 ccagttggct gaggagtacg aaggagaggt ggtcttcgtg ggcgtttcga acaacgacac
     7561 cgtcgccgac gggaaaggat acgtaaccga attcgacgtc ccctacgcga tggcacacgc
     7621 tcctgaggtg tgggacctgt acgacgtccc ctaccagccg gtaacgatcg ttctcgacgc
     7681 cggtggcaaa gaagtggaga gattcgacgg accggtcgaa actgaaccgt tacgaacggt
     7741 gatcgagaaa actctctgac tcagcggtcg cggcgaccgg ccagattctt gtcgacgatg
     7801 gccacgatga tcaaaacgaa ggccgccagc cgcagtccgt agatgtaggt cgtcgcctcg
     7861 taggtcgccg gaaccagcgc caggagcact ctgttgatcg cgaaggtcag gaacgctatg
     7921 ccgaagatca tgaagagtcg atcatggctc tggcgccaga acttggcgaa gaacaatgcc
     7981 gccacgagac agcccatcgc cgtaattcca tacaagaatt cgttcatggc atcacgacgg
     8041 atcgtccgat tcccagatga aaccgtatag aaggcatgcg atcccaatga cggcgggtgt
     8101 gtgtctaaga agagaaaggt cgacgtgagg caccactacc ttgtcgaaga acaggatccc
     8161 attgtttaaa gcgaagccca ggaaacacag agagctccag aacaacagcc ttagcctcga
     8221 atgcagatag gcccggatca acaggacggc acagatgatg ctcgtgaccg cacacagcag
     8281 gtaaacggtt tcagccatcg ctactcgtcc tcgtctctca atctgaacgc gtcggcgaag
     8341 ccgcgcacgc tgggggacgg cccggagaat atgagcccga tgaccgtagt gcgtcgcttc
     8401 gcgtaggtct ctgcaacatc gtcgatcgtc ttatccatat cggccgactc cggggaatag
     8461 gtaaatgtgc ctcccttgat atcggcggcg agcaggccgc gacccgcaag ttgacgcagt
     8521 cgcaagtcgg ccgattcgtc ggacagagtg agggcccgcg ccacatcctt ggtcgaccaa
     8581 gctttctcgg gtgctgcacg agcgagtagc agcacctcca agagctcgac cgaatcgatg
     8641 aagcgcgcca gaaaatcccg gacgttcttt gatacgtcct tttgccccac cacccgacca
     8701 tagaccagta aggtccccat ctgatcgaat acccattgca caaaggacta gagcgcgtct
     8761 cgtatctgct cgggacgtgg cgcggcgagg gcaagggccg ctatccgacg atcgacgact
     8821 tcgcctatgg ggaagaggtg aagttctggc acgacggacg gccgctgttg ttctacgcgc
     8881 agcggacgtg gaatctagag acggacggcc cgatgcattc cgaaaccggg ttctggcggc
     8941 cacagcgcga cggatcgatc gagatcgtgc tcgcgcatac gttcggctgc gtggagatcc
     9001 aagaaggaac ggtcgaaggg aaccggatcg gcaccaagag caaaggagtc gtctccactt
     9061 cgtcggccaa agacgtcgtc gccctcgcac ggcactactc tgtggacgga gacacgctca
     9121 gctacgagct cgagatgcag ttcgacgaca acgagctaca gaaccaccta tcggggacgc
     9181 tgacgcgaca gagttagtcg agttcttggg ctacgagcga gccgaagtaa gcggtgccgg
     9241 cttccatcgc gagccagaac agtccccggc ccttcctcaa gtcgcgccag ttcgcgagcg
     9301 tggcgagagc gtctccggcg tccgagacca cgccggcttc gatccaaccc cgcacggagc
     9361 cgcctttctc caacgccgtc aggatgccga tcccgatcgc gatatcccgt gcccccatac
     9421 ctcggatcgc aaggttcgtt gttacctcat cggcctcctc accggtccaa ccacgagccg
     9481 accgatgcgg catcaggaac agaacgccgc cgacaaagat gcgcatgaac ccgagggcac
     9541 gagcaagctc tctctcgtcg aactcctcgt cgaagatatt cacccctcca cctccatcgt
     9601 caacgctttg cgggcaagct cacgatcgtg ctcccacgcg aactcaagcc catccaattc
     9661 ctcaagtgat acccaccgcg catcggccac atcacttgca ggggtcggcg tccccgaagt
     9721 caatcgagcc acgaagccga tggctaggac ccagtcgtct tcttccccat aggtatgcgt
     9781 caccatatga acgaagtcgg actgggccac gtcgacctcg atatcgagtt cctcacggag
     9841 ctcccgccgc agggcatcga tcacgtcctc gccgagctgc aggaagcctc ccggcacgtc
     9901 gaatttccct ttatacggat ccgaaccacg taccgtcacc agagctcgtc cgtccttgac
     9961 gatcgcggcc cccaccgagg gagacgagtt gcggtaccag ttccggccgc acgagccgca
    10021 tatccgttcc tcctcgtcac cgacatccac ttcgctcgcg cacgcgggac agaacttgaa
    10081 cggtttgggc atcctcaacg ctcccgagaa ggtttagctg ccactccgcg ctcggcctct
    10141 tcgtagcgag cgcaggcata aagcaggtcg gagactcgat tcacgtagcg gaggacctct
    10201 ggattcggga tcagatcggc gcgctgcatg gctacggcaa gtcgctcggc cctgcgcacg
    10261 atcgagcgag cgagatccaa tgcggccgaa cccgaggttt cacccggtag caggaactct
    10321 tgaggaagtg ggtgctcctc gagcagagcg tcgatgtcgc gctcggcctc ttctgtcata
    10381 gaggcggtca ccttcgagat gccttccttc agcttgtcgt gttgatcggc gtgggtggca
    10441 agctcggccc ccgcaacgaa catctcccgt tgtacacgca cgacgatcga ctcgatgcgg
    10501 tcgacaagcc cggcggcccg cgccaaaccc agcgcggaga ccgtttcgtc gagagttccg
    10561 tagacctcgg tgcggatgtc gtccttggtg acacgacccc catagaggag gccggtggtc
    10621 ccgtcgtcac ccttcttcgt atagatcttc ggcacgaagc gacactaaat gggcccgccg
    10681 gaaggcgggc ccatttattg ctcctgaaac gaaagactta tctagccgca gtcggtagcc
    10741 gtgttccccg actgatcggc gctcgagtcc tggttggtct cgttaccgca atgcactcca
    10801 gttccgtcca cgcgcgcgta gccgttcgcg ggctcggcgt tgctgttgtc cccgtcgatg
    10861 gctgcgtacc cgtcctcggc atcgacggtt gccgagccat cgatcgggaa tgcagaggtg
    10921 ttatccgcgc acgcctccgc cccgttcgtc cctgcgtaga catatgcgac gccggcagcg
    10981 gacgtcgaac tgtcctcatt gcaatcgacc tcgttcgcag tctcctgcga gccactcggc
    11041 ccagccagtg ccgtgccgcc catcaagaag aacaggagga acaacgacag agacgctgct
    11101 gcgggtacag ccgttctgcg catgcgacaa ctcctttccg ctcggggtgc aggcgcgtcg
    11161 aaagttcacg tcgtcgggat catccgatct gccctgcttt gttcttcggc ggtccccgcc
    11221 tccgagaggt cctaaacctg ggattaggtg ggattcactt gccggccgtt gcgcgccggt
    11281 cggagtccaa gcaacgcgag cagccccacg gcccctagaa gcgtgaagcc cagaacggcg
    11341 atcgggaccg atacgggcag tacgtcgaag gtcgcgcgcc cgctgtaggt ctggatcaag
    11401 gttgcggccg cgagcgcggc ataacccatc gcggccatgc gcaccaaacg gacgcgtcgt
    11461 tcttcggtcg tatcggcccg acccaaaagc accgcaagcg ccggtagcac ctggatcccg
    11521 tgcatggtca gcgcgtgcgg tgtcttcatc gcgccggcat ctccccacac gtttccgtac
    11581 acggcgatca tcatgattcc cgtcgcttga ctgagtagca ggatcaggaa ccccaaccgg
    11641 atccccagcg ccaagctcgg tggtgcttca agtctgatga acgcccgcac ggtcaggtag
    11701 acgatcacga agatcatcac ccccaccatc agtcccatga tgccgaagac ggaggaatcg
    11761 aaggtggtct tgtcgttgaa gtgcgattcg accccgcgcc acttctgcat gctgacgagg
    11821 aacacctcga ggagcgcgga cacactcatg acaccggccg cgatccatcc cgacaaaggc
    11881 ttcagcttga ggaacgacat cacccacgtc aatgtcacga ccgtgatgcc gaacgagaag
    11941 ccgaagagca ccggcttgcg ccacgagatc ggtccctccc acggcccccc cttgatgagg
    12001 aacacgatga agtggaaggc accggcggcc agcaacaagg caccgcaggc gtacgcaacc
    12061 ttctgatagc gggtgggcgg aggaagggcc aatgagctag tgcgagtggc tgtgccccgc
    12121 attgatgttg ggaagaagag gtaaagagat ccgcttagcg ggccgcggct tcggcgggat
    12181 caggttcacg atctccttcg ccgtggccat gagctgctga gccacctccg accccggctc
    12241 ttgcagaaca accggtgtcc cgttgtccgc gaactcccgt gcttgtggat cgagtgggat
    12301 ctcgccgagg acgggaacgc cgagacggtt cgcgagcgag gcacctccac cctcgccgaa
    12361 gatccggtac tgcttgtcgt cgtcgccgcg aaagtacgac atgttctcga tgactccgag
    12421 cacgctgagg cccgtcttct cggccatgaa gcccgcgcgc tgcgcgacct tctcggccac
    12481 gggctgcggc gtcgtcacga tcaccatgtg cgcgccgggc aggaactgcg cgatcgagat
    12541 cgacacatcg ccggtgcccg gagggagatc gaccagcagg tagtccggct cgtcccagtg
    12601 gacgtcggta aggaactgtt gcaaagcctt gtgcagcatc ggcccccgcc acacgaccgg
    12661 attgtcctcg gcggtgaaga gtccgatcga gatgaccttg acaccgaatt tctcgggagc
    12721 catgaccatg tcgccgacca cggtcgggcg ctcgtgtaga tcgaacatgc gcggcacgga
    12781 gaaaccccac acgtcggcat cgagcagacc tacggtgtgg ccgagctgcg cgagagcaac
    12841 ggcgatgttg acggtcgtcg tggacttacc gacaccgccc ttgccggagc cgatcgcaag
    12901 cacctgcgtc ggagagtcgc tgcgcgcgaa gggggccggt gtgtccttgc ggagaccacc
    12961 gatcagggat tcccgctcct cttcggtcat cgaggtgagc tcgacgcgca cggcgtttat
    13021 ctcggagaac gactccttca gcttcgccgg tatgaccgtg tggaagtaat ccttaagagg
    13081 acaaccgggg atagtgagcg cgaccagaac ggttacatcg gatccctcga tcgcgatgtt
    13141 ccgcaccata ttgaggtcgt tcagccctcg atggagctcc ggatcctgga tctcccctag
    13201 tacctgggag atctgttcct cggtaggaaa gcccatgcgc gaaaggttaa gcgaccggga
    13261 cgcgactcga ttgtcgtatt tggtcgaacc cccttccgtt gggcagggtc cgtccctagg
    13321 ctggcccgat gaaacgatct ggcgtcctca ccttgcttgt ttcggttgtt ctcatgtcga
    13381 tgtccgttgc cgcaggagca gccgggcagc cacctgagtc gcgcgaggac cgctacacgg
    13441 ttaaggcggg gaggcaactg gtggtttcgg ctgccgatgg tctgctcgcc aacgactccg
    13501 atcccgaggg cgacaagctc acggcggtcc tgagccacca aaggccaccg gggaaactct
    13561 cactgcgacc ggatgggtcg ttccggtaca caccgatcag cagcgggggc aacgacttct
    13621 tctcttacac ggcattcgac ggacagagcc actccccacc cacgttcgtg atcatcagag
    13681 tcgacgcggc gccgatcacc caacccgact cgtatggcgt gatagcaccc gccccc
//
LOCUS       DATMED010000227         5882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4781850, whole genome shotgun sequence.
ACCESSION   DATMED010000227 DATMED010000000
VERSION     DATMED010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781850"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1520
                     /locus_tag="VNP73_05835"
     CDS             393..1520
                     /locus_tag="VNP73_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWL65478.1"
                     /translation="METTDPTTAEQAHITVRRLVRRREKRLVAGVAGGLADYSGLDPA
                     IFRILFVVLSFAGGAGAFLYLLGWGLVPEAGERFSKGERFVRRFRRTSWLGVAFLVAA
                     ALFLFSETGLWTPTFLWAVALIVIGIVLLRDDIADETARSKEISELEPDVVTDSFETE
                     VLRVRRERRPRSPLGLLTVAAALLVVGIGSVLSNTGVITLELADHFALGVATIGCGLL
                     VGTLWGRARLTIVLGVLLVPFLLAASLVEMPLEGDLGVLVYNPRSVDAVEAGYKMAVG
                     EMTLDLSDVPFDEGQTRIPITLAVGELDIYVPRDVRVSLDGTVDLGGMQTFGRLVDGE
                     NLVIDRSFGDVGADKEVVLDIEAGIGSVDARWTGARERSRR"
     gene            1517..1756
                     /locus_tag="VNP73_05840"
     CDS             1517..1756
                     /locus_tag="VNP73_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65479.1"
                     /translation="MKGHQFDPVSFLFGLLFAGLGLVFLFGDFTLLDLGWRWIWPVPV
                     IFTGVLVLSLALNRYFKQTQDDQSESSESLSSVVR"
     gene            complement(1714..2160)
                     /locus_tag="VNP73_05845"
     CDS             complement(1714..2160)
                     /locus_tag="VNP73_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65480.1"
                     /translation="MTVQRIKAEFDARWESWGVALPEEDLTARRGGALRQVEGSGLIR
                     YAFGKSEDDREYLEYYSFHRIGGDSHGRIYDTGEFERLDSLQTMYISSGTEEEVRRRK
                     DEMYERNRKLLEDLHNAGLMDGGPVPHSFQINAYLTTDDRDSDDSD"
     gene            complement(2160..2567)
                     /locus_tag="VNP73_05850"
     CDS             complement(2160..2567)
                     /locus_tag="VNP73_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65481.1"
                     /translation="MSSTQESIDATFYCSECGKAASTVRFVPSERQDPRIDANRDLTK
                     NPLPGISITGGPVPIAINVVGDWAPAAIEALRNQDAAALYAVNNEFAPFWCRTCEKSY
                     CADHWVTLDLYDEGFFDYTRGTCPKGHQQMLMD"
     gene            complement(2564..3565)
                     /locus_tag="VNP73_05855"
     CDS             complement(2564..3565)
                     /locus_tag="VNP73_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65482.1"
                     /translation="MAQQEVRTHADRLEIGHLSVTFHRTLRIPDDGKNYPLPPSLGAF
                     PIRKVSDYADRVPDDWREHGGVFIPMYQREALWMSFHSVTGKYGAVKVGIGKIDALTG
                     KKWSEKLHARRQDYMVVPGQPWLDGINAGKEFIKQFVAMPLGMGYTVEGQLTGKEDVG
                     GIQFKVFPAKPGREPEPPVFLHHDQLAPASAKHEYSMPAAQSAAPAMGLGAGGKMTQS
                     IYPDPHGIDIWDPERSGRCFVHIATSMVWRDITGEEPPATPVSAELYTQYGFPWFELY
                     DEDKGDIGASGKLGGVKTVKEMDKEKGFGTQQDDSTVDIDPNQIKNADPTKVLDGKW"
     gene            3757..4179
                     /locus_tag="VNP73_05860"
     CDS             3757..4179
                     /locus_tag="VNP73_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HWL65483.1"
                     /translation="MRTIGNVLWLLLGGIELALGYLVAALLAIVFIVTIPFAVPAVRL
                     ALFCLWPFGRTLVKKPEAGAGSAIGNVVWLILFGWWLALGHLVAAFLMAITIIGIPFA
                     IAHTKLAVTALWPFGTEVVSIDSLPVGSNSIVVGGQPN"
     gene            complement(4132..4854)
                     /locus_tag="VNP73_05865"
     CDS             complement(4132..4854)
                     /locus_tag="VNP73_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWL65484.1"
                     /translation="MDVAIIGGHGKIALRLARLLTKRGDRVWSIIRKPEQSGHITAVD
                     ATPVWCDVETTSAVGLAAAIGKVDAVVFAAGAGPGSGPGRKETVDYGCAVKLIQAAKD
                     NGIDRYVMISSVGADPEAPGDEIFDVYLRAKGRADQALIDSGLAYTIVRPVSLTEDPG
                     DGKVFIGDSVPRGKIPRDDVAVVLAAVLNQPATIEQTFEVTDGEEPVEVALRAVGKDV
                     VIDLREKSVRLAPDDDRVTPDR"
     gene            4923..5630
                     /locus_tag="VNP73_05870"
     CDS             4923..5630
                     /locus_tag="VNP73_05870"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HWL65485.1"
                     /translation="MNQPPEGWYDDPQVPGQQRYWDGTAWTDQVQPKLLFDGVLWLPK
                     VVRSKEQRLVITASELWWGDEYLRWDEVIAFTQLTQVMVGIPYIHELVLQCHDRNVPI
                     GFMARVKRDPIGDRAYAVIIEILRQTLGVRVLTGLMEMLANGEPIRLAGLLLSPEGFT
                     REDKDDPMIPWTDYAGMKVDAGDSIYINLFRVKDGGKRKRAASVSTDMLRSWVVGPIV
                     EEHARRYGAPVTPDATS"
     gene            complement(5620..>5882)
                     /locus_tag="VNP73_05875"
     CDS             complement(5620..>5882)
                     /locus_tag="VNP73_05875"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWL65486.1"
                     /translation="LRPAGEGSILGHIQVGSEEAALHMLKDEGVLSENELLDAKNALW
                     GEPPVQFRKMTRMLSELHHLYKEGILTESEFNSKKWEVLSRK"
BASE COUNT          969 a         1771 c         1895 g         1247 t
ORIGIN      
        1 agggctagcc atgcgaccgc gtaaaggagt gcgccggcgc cttctgcgag ggataggacc
       61 acaaaggcgc tgcgcacaat cgcggggtcg atgccgaagg cgtctccgat cccgcctgca
      121 actccggcga cgactcggtg attggtactg cgcgggggca gcgcatttcg aactcgtcta
      181 gcgaatctcg tgaagcggcc cgcgtctgga cccggacggc tcaggttcgg tgcgacgtcg
      241 acgatgggat tccctcctta tctcggtcga tggtgtcaca gacgagtgga ctcgaccatc
      301 ggggacgacc cggaaccgag ggacatggat tcagggtttc ttcagggtca tcccccatag
      361 gcaaggaccg gcccggcccg gatgatgggc ccatggagac gacggatccc accactgcag
      421 agcaagcaca cataaccgtg aggcgactgg ttcggcgccg cgaaaaacga ctggttgccg
      481 gagttgcggg tggcctcgcc gactacagcg gactggaccc cgcgatcttc aggatcttgt
      541 tcgttgttct ctcgttcgcc gggggcgcgg gggcattcct ttatctgttg ggttgggggc
      601 tggtgccgga ggcgggggag cgtttctcga agggagagcg cttcgttcgc cggttccgcc
      661 gcacctcttg gttgggggtc gcgttcctcg ttgcggcggc gcttttcctc tttagtgaaa
      721 cggggctgtg gacaccgacg tttctgtggg ctgtggccct catcgtcatc gggatcgtgt
      781 tgctacgcga tgacatcgcc gatgagacgg cccgatcgaa ggagatatcg gaactcgagc
      841 ccgatgtcgt gaccgactcg ttcgaaaccg aggtgttacg tgtcaggcgc gaacgccggc
      901 cacgctcgcc tctgggtctc ttgacggtcg ccgcggcttt gctggtcgtg gggatcggct
      961 cggtgctctc gaacacagga gtcatcacgc tcgagctcgc ggatcacttc gcgctcggcg
     1021 tggcgacgat cggatgcggt cttctcgtcg ggacactgtg gggtcgagct cggttgacca
     1081 tcgtgctcgg cgtgcttctg gtgccgttcc ttctggcggc gagcctcgtg gagatgccgc
     1141 tcgaaggcga cctcggcgtc ctcgtctaca acccacggtc ggtcgatgcg gtcgaggccg
     1201 gatacaagat ggcggtgggc gagatgactc tcgacctctc tgacgtgccg ttcgatgagg
     1261 ggcagacccg gatccccatc acactggcag tcggtgagct cgacatctac gttccgcgcg
     1321 acgtgcgggt ttcgttggac ggaacggtcg atctgggcgg gatgcagacc ttcggcaggt
     1381 tggtcgacgg cgagaacctc gttatcgacc gttccttcgg agatgtcggc gccgacaagg
     1441 aagttgtgct ggacatcgag gccggaatcg ggagcgtaga tgcgcgctgg accggcgctc
     1501 gggaaaggtc gcggcgatga agggacatca gttcgatccc gtctcgttcc tgttcggact
     1561 tctgttcgcc ggactcggcc tcgtcttcct gtttggagac ttcaccttgt tggatctcgg
     1621 atggcgatgg atctggccgg tcccggtcat cttcacggga gttctggttc tgtcactcgc
     1681 actgaacagg tacttcaaac agacccagga cgatcagtcg gagtcgtcgg agtccctgtc
     1741 gtcggtcgtc aggtaggcgt tgatctgaaa cgagtgcggc accggcccac cgtccatcag
     1801 gccggcgttg tgcaagtcct cgagtagctt ccggttgcgc tcgtacatct cgtccttgcg
     1861 ccggcgcacc tcttcctcgg tccccgaact gatgtacatg gtctggagcg aatcgagccg
     1921 ctcgaactcg ccggtgtcgt agatgcggcc gtgcgagtcg ccaccgatcc ggtggaacga
     1981 gtagtactcg aggtactccc tgtcgtcctc gctcttgccg aaggcgtatc ggatgagacc
     2041 cgacccctcc acctgacgaa gcgctccgcc acgacgtgcg gtcagatcct cttcaggtaa
     2101 cgcgacgccc caggattccc acctggcatc gaactcggcc ttgatccgtt gcacggtcat
     2161 caatccatga gcatctgttg atgccctttg ggacacgtcc cccgggtgta gtcgaagaat
     2221 ccttcgtcgt agaggtcgag cgtcacccag tggtcggcgc agtaggactt ctcgcacgtg
     2281 cgacaccaga atggcgcgaa ctcgttgttg accgcgtaga gggccgcggc atcctggttg
     2341 cgtagagctt cgatggcggc cggggcccag tcgccgacga cgttgatcgc gattgggacc
     2401 gggccgcccg tgatcgatat ccccggcagg ggattcttcg tcaggtcgcg gttcgcatcg
     2461 atccgaggat cttgcctctc gctcgggacg aaccgaacgg tcgaggcggc cttgccgcac
     2521 tcggagcagt agaaggtggc atcgatgctc tcttgtgtcg agctcaccac ttcccgtcga
     2581 gaaccttcgt cggatctgcg ttcttgatct ggttcgggtc gatatcgacg gtcgagtcgt
     2641 cctgttgcgt gccgaagccc ttctctttgt ccatctcctt gaccgtcttg acgccgccca
     2701 acttcccgga cgccccgata tctcccttgt cctcgtcgta gagctcgaac cagggaaagc
     2761 cgtattgcgt gtagagctcg gcgctgacgg gagtggcggg cggctcttcg ccggtgatgt
     2821 ctcgccacac catgctggtc gcgatgtgga cgaaacagcg accggagcgc tccgggtccc
     2881 agatgtcgat gccgtgaggg tcggggtaga tgctttgggt catcttgccg cccgcaccga
     2941 gacccatcgc aggtgcagcc gactgcgctg caggcatgga gtactcgtgc tttgcgctcg
     3001 caggcgcgag ctggtcgtgg tgcaagaaga cggggggctc cggctcacgt cccggcttcg
     3061 ccgggaacac tttgaactgg atgccgccga cgtcttcctt gccggtcagt tggccctcga
     3121 ccgtgtagcc catgccgagt ggcatcgcga cgaactgctt gatgaactcc ttgccggcgt
     3181 tgatgccgtc gagccacggc tggccgggaa cgaccatgta gtcctgtcgc ctggcgtgaa
     3241 gcttctcgct ccacttcttg ccggtgagcg cgtcgatctt gccgatgcct accttcacgg
     3301 ccccgtactt gccggtgacg gagtggaacg acatccacag cgcctcccgc tggtacatcg
     3361 ggatgaagac cccgccgtgc tccctccaat cgtcgggcac ccgatcggcg taatcggaca
     3421 ccttgcggat cggaaaggct cccaggctcg gcggcagcgg gtagttcttg ccgtcgtcgg
     3481 gtatccgcag cgtccggtgg aacgtgacgg atagatggcc gatctcgagg cggtcggcgt
     3541 gggtgcggac ctcttgctga gccactgctc ctccttagtc gtgcttagtg tgattcccca
     3601 tgctcatgtt gacgaggggg tgcgacaaaa agttcctggg gccgaatacg aggtcgcttt
     3661 tcccgatttc ggataggttt cgcagcggcg aaatcgactt atagggggct gcaaatgaga
     3721 aagagactcg cgttcgtcgc ggcctagtct tgtgtgatgc gaacgattgg aaacgtgctg
     3781 tggctcctgc tcgggggaat tgagctagcg ctcggctatc tggtggccgc cctcctggcc
     3841 atcgtcttca tcgtcacgat cccgttcgcc gtccccgcgg tgcgcctcgc gttgttctgc
     3901 ctgtggccgt tcgggaggac tcttgtcaag aagccggagg cgggggccgg atcggcgatc
     3961 ggcaacgtgg tgtggctaat cctgttcgga tggtggctcg cgctcgggca cctggtggcc
     4021 gccttcctga tggcgatcac gatcatcggc atcccgttcg cgatcgcgca cacgaagctt
     4081 gcggtgacgg ccttgtggcc cttcggcacc gaggtggtgt cgatcgactc gttaccggtc
     4141 gggagtaact cgatcgtcgt cgggggccaa ccgaactgac ttttctctaa ggtctataac
     4201 gacgtctttt ccaaccgccc gaagtgcaac ctctaccggc tcttcgccgt cggtgacttc
     4261 gaaggtttgc tcgatcgttg ccggctggtt cagcacggct gccagaacca ccgcgacgtc
     4321 gtcgcgcggg atcttcccgc ggggaacact gtcgccgatg aacacctttc cgtcgcccgg
     4381 atcctctgtg agagaaaccg gtcgcacgat cgtgtacgcg agtccgctgt cgatcaaggc
     4441 ttggtcggcg cggccctttg cccggagata gacgtcgaag atttcgtcgc cgggagcctc
     4501 gggatcggct ccgaccgagc tgatcatcac gtagcggtcg atcccgttgt ccttggccgc
     4561 ttggatcagc ttcacggcgc agccgtagtc gacggtctcc ttgcgcccgg gcccgctgcc
     4621 ggggccggct cccgccgcga agacgacggc atcgaccttg ccgatcgccg cggccagccc
     4681 aacggcgctg gtcgtttcta cgtcgcacca gaccggggtt gcgtcgacgg ctgtgatgtg
     4741 acccgattgt tcgggcttgc ggatgatcga ccagacgcga tcaccccgtt tcgtcaataa
     4801 gcgtgcgagt ctcagcgcga tcttcccgtg cccaccaatg attgcgacgt ccattccccg
     4861 cccctttgct attccgccag atgacactgg ttcgccagcc tcgccagtgc tgaaatagag
     4921 agatgaacca gccgccggaa ggatggtacg acgaccccca agtgccgggt caacaacgct
     4981 attgggacgg aaccgcgtgg accgatcagg tgcaacccaa gcttctcttc gacggggtgt
     5041 tgtggctccc caaggtagtt agatcgaagg aacaacgact ggttataact gccagcgagt
     5101 tgtggtgggg tgacgaatac cttcggtggg acgaggtcat cgcattcacg cagttgaccc
     5161 aggtcatggt ggggatcccc tacatccacg aattggttct gcagtgccac gatcgcaacg
     5221 tgccgatcgg attcatggct cgcgtcaaac gagatccgat cggcgatcgc gcttacgcag
     5281 tcatcatcga gattcttcgt cagacgctcg gggtcagggt tctgacgggg ttgatggaga
     5341 tgctcgccaa cggcgaaccg atccggcttg ccggcctgtt gctctcaccc gaagggttca
     5401 cccgggagga caaggacgac ccgatgatcc cctggaccga ttacgcaggg atgaaagtgg
     5461 atgccggcga ctcgatctac atcaatctct ttcgagtgaa agacggtggc aaacggaagc
     5521 gagcggccag tgtgtcgacg gacatgctca ggagctgggt tgtgggcccg atcgtcgagg
     5581 agcacgcacg tcgctacggc gctccagtga cgcccgacgc tacttcctag acagaacctc
     5641 ccacttcttg gagttgaact ccgactcggt gaggatccct tccttgtaga ggtggtgtag
     5701 ttcggagagc atgcgagtca tcttgcggaa ttggaccgga ggttcacccc acagcgcgtt
     5761 cttcgcgtcg agcagttcgt tctcgcttag aacgccttcg tctttgagca tgtggagagc
     5821 ggcttcctcg ctgcccacct ggatgtgacc gaggatcgag ccctcgccgg cggggcggag
     5881 tt
//
LOCUS       DATMED010000228         5019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1712188, whole genome shotgun sequence.
ACCESSION   DATMED010000228 DATMED010000000
VERSION     DATMED010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712188"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VNP73_05880"
     CDS             <1..575
                     /locus_tag="VNP73_05880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL65487.1"
                     /translation="GTLPMMWWFFTGPEGLSEAISSALGEQGFVLESDRPSMELALGS
                     FEPPVPAAPVEIRRVSDEGEFRTWSDVVGLAFEAEDHWSSMSTRSFLRLGWDDPAPFR
                     HYLGTLEGRPVGAATLSVGAGVAGLANIATVPDQRRRGFGRAITSEALLDAKKMGLGT
                     AALSADEDGSYLYRSLGFKEIGRHFTFVRR"
     gene            587..1108
                     /locus_tag="VNP73_05885"
     CDS             587..1108
                     /locus_tag="VNP73_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWL65488.1"
                     /translation="MEAWQAIDSIRVVREFDERPISTDDLTKIVNAGRRAGSSKNLQR
                     WAFITITQRDRLTQLSKSGPWAGHIAGATTAIALVTPDPNGSDQPLSVMWDLGRAAQN
                     MVLVAWDLGIGSAPATVYEHDVVKELLQIPADHHCEYILSFGYPANTGELKTPKRSGG
                     RAKLQEVLHEEVW"
     gene            complement(1137..2678)
                     /locus_tag="VNP73_05890"
                     /pseudo
     CDS             complement(1137..2678)
                     /locus_tag="VNP73_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
     gene            complement(2706..2975)
                     /locus_tag="VNP73_05895"
     CDS             complement(2706..2975)
                     /locus_tag="VNP73_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWL65489.1"
                     /translation="MRGYYDDKDEVLARLRRIEGQVRGLQRMVESDEYCIDILTQISA
                     ASNALKKVGVGLLDDHIRHCVVEGKSPGKDMVAEATAAIERLVRS"
     gene            complement(3064..3876)
                     /locus_tag="VNP73_05900"
     CDS             complement(3064..3876)
                     /locus_tag="VNP73_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65490.1"
                     /translation="MRRLAFALADGTVVAKRNLIKIKRVPEILIWTLISPIMFVVLFG
                     YVFGSSIDVRGGVNYREFFIAGIFAQTVIFGSTFTGVGLAEDMTKGVIDRFRSLPMAR
                     SAVLTGRTGSDIFYNLISIFMMAMTGLLVGWRIRTGVVEAIGGFLLLLVFAYAISWIM
                     AFVGLKVSSVEVVNNASFMFIFPLTFVADTFVMAERLPGVLKTFAYWNPVSSVARGAR
                     EAFGNTGGIPPPDVWPLQNPVLYTLIWVAIILAIFIPLSIRQYEKAAARATT"
     gene            complement(3873..4883)
                     /locus_tag="VNP73_05905"
     CDS             complement(3873..4883)
                     /locus_tag="VNP73_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL65491.1"
                     /translation="MIRTEGLVKRYGDVVALDGLDLSVEKGVVLGLLGPNGAGKTTAV
                     RILTTLLQHDGGIAEVAGIDVRKEPDKVRERIGLSGQNAAVDEYLTGYENLNMVGRLY
                     HLGAERSKKRARELLERFDLEDAADRPTKTYSGGMRRRLDLAAALVASPDVVFLDEPT
                     TGLDPRSRAQMWDTIHELQRGGSTVLLTTQYMEEADRLADDIVVIDHGREIAHGTGDQ
                     LKTKLGGERIEVVLADAADVQPASSILAGVAVGEVQVDATARKLNVAVSGGPVALKEV
                     LNRITDANIGVVDIGLRRPTLDDVFLSLTGHAAEEVAEEGDQPDESKKRDRAARQKEA
                     VR"
BASE COUNT          946 a         1659 c         1490 g          924 t
ORIGIN      
        1 acggaacgct tccgatgatg tggtggttct tcaccggacc cgaaggtctg tccgaagcca
       61 tctccagtgc tctcggcgag caaggattcg ttctcgagtc cgaccgtccg agcatggaac
      121 tggctctcgg atcattcgaa ccaccggtgc ccgcggcacc agtcgagatc cgccgagtct
      181 ccgatgaagg cgagttccgg acctggagcg atgtcgtggg tctggccttc gaagctgagg
      241 accactggtc gagcatgtcc acgcgttcgt tcctccggct cggctgggac gacccggccc
      301 cgtttcgcca ctacctgggg acgctggagg gtcgaccggt gggagccgcg acactttcgg
      361 tcggggcggg cgtggccgga ttggcgaaca tcgcaacggt tcccgatcaa cgacgacgag
      421 ggttcggacg agcgatcact tctgaagctc ttctcgacgc caagaagatg gggttaggga
      481 ccgccgcgtt gtctgccgac gaggacgggt cgtatctata tcggagtctc ggcttcaagg
      541 agatcgggcg tcacttcaca ttcgtgcgac ggtaagggag ggctccgtgg aggcttggca
      601 agcgatcgat tcgatccgag tggtgcgcga gttcgatgag aggccgatct ccactgacga
      661 tctcacgaag atcgtcaatg ccgggcggcg cgccggcagc tcgaagaatc ttcaacgatg
      721 ggccttcatc acgatcacac aacgtgaccg gctcacgcag ttgtcgaagt cggggccctg
      781 ggcgggacac atcgcgggcg cgaccacggc gatcgcactc gtaacgcccg atcccaacgg
      841 gtcggatcag ccactgtcgg tgatgtggga cctgggccga gccgcccaga acatggttct
      901 ggtcgcctgg gatctgggga tcggaagtgc cccggctacg gtctacgagc acgatgtcgt
      961 gaaagagcta ctgcagatcc ccgccgatca tcactgcgaa tacatcctgt cgttcggtta
     1021 tccggcaaac acaggcgaac tgaagacacc gaaacgatcc ggcgggagag cgaagttgca
     1081 agaagtactt cacgaagagg tctggtgaac cactaacggc cgcgtagccc ggccggtcac
     1141 caagcgtcga tgaagggccg tttctttccc gttcgcgggg gggtgggtgg aacggacgac
     1201 aacacgttcg agatgaccgt ccgggtttcc gccgggtcga tcacgtcgtc gatctcgaag
     1261 ttcgaggcca ggttcagtgc cttcccgcgc tggtaggcct cggccaccat ggcctcgaac
     1321 atgcgctccc tctcggcttc gtcctcgatc gcgtccagct ccctgcggaa cccgagccga
     1381 acggcccctt ccaggttcat cccgccgaac tcgccggtcg gccacgacac cgttagcaac
     1441 tgcgcgtgga acgagcctcc tgccatcgct tgcgcaccca gtccatatcc tttgcgaaga
     1501 acgatcgtca tgtaaggcac ggtgacgttc gcgccggcga cgaacatccg agcgaagtga
     1561 cgaacctgcg cctgctcctc ggctttgggc ccgaccatga accccggcgt gtcgcacaga
     1621 gagacgatcg gcagatcgtg ggcatcgcac agcgacacga accgcgctgc cttgtcggca
     1681 ccgtcactgt cgatcgcgcc tccgagacga cgcgggtcgt tggcaagtat cccgaacggc
     1741 cgaccctcga tccgtgccag tgccgtaacg atcgtccgcc cgaatcccga acgcatctcg
     1801 atgacgcttc cggtatccgc gaggatctcg atcgccggcc tcgggtcgta tgcggttcgt
     1861 cgatgtgcgg ggacgacgtc gcggagagcc gtctgatctg cgcactccca tccctctgtc
     1921 ggaccggcga agagacccac cagtctgcgg gccacgtcga ccgcttcctt ctcctcttgc
     1981 acgaggatgt cgacggcgcc gctcttcacg tgaaccgatg cgggccctac ttcctccggt
     2041 gcatgggttc ccaagcctcc ggcctcgatc atggccggcc cccccatccc gatattggca
     2101 tcggccgtcg ccacgatgat gtcgcaacaa cccagcagcg ccgcattccc tgcgaagcag
     2161 aaccccgccg ccacacctat cgtcggcacg agaccgctaa gacgagcgaa cagagcgaat
     2221 gccatcgtct cgagcccgga taccgtcggc acatcggtat ctccggcgcg gcccccaccc
     2281 ccctctgcga agagaaccac cggcagccgc aaacgctcga tcacctcgaa gagccggtct
     2341 ttcttgcggt gattgaccgc accttgcgtt cccgccaaca ccagatagtc gtaagaaagg
     2401 atggcgcacg ggagtccgtc aacgcgaccg atcccaccga caagcccgtc cccgggagtc
     2461 cgagcgatga gatcctcgac gggacgccta gagcgttgcg ctgcgatcgc cagacctccg
     2521 tattcgacga aggaccctgc gtcgcacaga tcctcgacat tctcgcgagc ggatcgacga
     2581 cccgcagcat gccgtttggc tacggcctct gcgcgcgcgg catcgcgggt cgtggcgcga
     2641 cgttcgaaaa gctcgccgag ctcgggccgg acctcgtctt cggacatcgg agtaacccta
     2701 gagggtcagg atctgaccaa tcgttcgatc gctgcggtcg cctctgccac catgtctttc
     2761 cccggtgact tgccctcgac cacgcaatgt cggatgtgat cgtcgagaag acccactccg
     2821 accttcttga gagcattcga ggccgcgctg atctgcgtca atatgtcgat gcagtattcg
     2881 tcgctctcga ccatccgttg gagcccgcga acctggccct cgatccgtcg cagacgagcg
     2941 agaacctcgt ccttgtcgtc gtagtaaccg cgcacgcgtg tcccctttcc gagtcgcgtg
     3001 cacaccaccc tataccctag gggggtatca catcagaggg ggactaacgt tcggggttaa
     3061 cgatcaggtg gtcgcccggg cggcagcctt ctcgtactgc ctgatcgaca gtgggatgaa
     3121 gatcgccagg atgatcgcta cccagatcag ggtgtacagc accgggttct gcaggggcca
     3181 cacgtcgggg ggcgggatcc ctcccgtgtt cccaaaggcc tcgcgggctc cgcgcgcgac
     3241 cgaggagacg gggttccagt acgcgaacgt cttcagcacg cctggaagcc gctcggccat
     3301 gacgaacgtg tctgcaacga acgtgagcgg gaagatgaac atgaacgacg cgttgttcac
     3361 gacctcgaca ctcgagacct tgagtcctac gaaggccatg atccacgaga tcgcgtacgc
     3421 gaagaccaga aggagcaaaa atccgccgat ggcttcgacg accccagtgc ggatcctcca
     3481 accaacgagc agaccggtca tcgccatcat gaagatggag atcaggttgt agaagatgtc
     3541 gcttccggtg cgaccggtga gcaccgccga gcgcgccatc ggcaaagacc ggaaccggtc
     3601 gatgacaccc ttcgtcatgt cctcggctag acccaccccg gtgaacgtcg agccgaagat
     3661 gactgtctga gcgaagatcc ccgcgatgaa gaactcgcgg taattcactc cccctcgcac
     3721 atcgatggag ctgccgaaca catagccgaa caggaccacg aacatgatcg ggctgatcag
     3781 cgtccatatc aggatctcgg gtactcgctt gatcttgatt aggttgcgct tcgctacgac
     3841 ggtgccgtcg gccagtgcga atgcgagtcg cctcatcgaa cggcctcctt ctgacgagca
     3901 gcacggtcgc gcttcttcga ttcgtcgggt tgatcgcctt cctcggccac ctcttcggcg
     3961 gcgtgaccgg tgagcgagag gaacacatcg tccaaggttg gcctgcgcag accgatatcc
     4021 acgacgccga tgttggcatc ggtgatccgg ttcaagacct ccttgagcgc gaccggcccc
     4081 cccgagaccg ccacgttcaa cttgcgggcg gtcgcgtcca cctggacttc cccgacggct
     4141 acgccggcga ggatcgagct ggccggctga acgtcggccg cgtctgccaa aacaacctcg
     4201 atgcgctcac ctcccagctt cgtcttcagc tgatcgccgg ttccatgcgc gatctcacga
     4261 ccgtggtcga ttacgacgat gtcgtcggcg agccggtcgg cctcttccat gtactgcgtc
     4321 gtcagaagaa ccgtcgagcc accgcgctgc aactcatgga tcgtgtccca catctgagcc
     4381 ctgcttcgtg ggtcgagccc ggtggtgggc tcgtccaaga agacaacatc gggggacgcg
     4441 acaagcgcgg cggcaagatc gagccgtctg cgcatacccc cggaataggt cttggtcggc
     4501 cgatcggccg cgtcctcgag atcgaatcgc tcgagcagct cgcgggccct cttctttgag
     4561 cgctcggccc ccaggtgata cagcctgccg accatgttga ggttctcgta accggtcagg
     4621 tattcgtcga ccgctgcgtt ctgtccggac agtccgatgc gctcgcgcac cttgtccggc
     4681 tccttgcgaa cgtcgatccc cgcgacctct gcgattcccc catcgtgttg tagcagggtc
     4741 gtgaggatcc ggaccgcggt cgtcttgccc gcaccgttgg ggcccaggag cccaagcacg
     4801 acccccttct cgaccgagag gtcgaggccg tctagcgcaa cgacgtcgcc gtagcgcttg
     4861 accaacccct ccgttcggat catctgactc actcaaccac ccctctctcg cccatcaagc
     4921 tctctttcta ctcctgtcgt aagacctccc tagatcgaaa aactcatcgg tctgccgatt
     4981 aattccgaag ccggatcccg tctatagaga taaccgacg
//
LOCUS       DATMED010000229         6723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_549883, whole genome shotgun sequence.
ACCESSION   DATMED010000229 DATMED010000000
VERSION     DATMED010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549883"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..333)
                     /gene="rpmE"
                     /locus_tag="VNP73_05910"
     CDS             complement(124..333)
                     /gene="rpmE"
                     /locus_tag="VNP73_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWL65492.1"
                     /translation="MKQGIHPEYMETIVTCSCGNTFTTRSTKSELHVELCNQCHPFYT
                     GKQKLVDSGGRIEKFERRYGRRDKK"
     gene            complement(330..482)
                     /locus_tag="VNP73_05915"
     CDS             complement(330..482)
                     /locus_tag="VNP73_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65493.1"
                     /translation="MFLGLGLPEWLLIIGLVGGLWVTFKVMARASVAYEKARQAREQR
                     EQEDQE"
     gene            complement(496..2094)
                     /gene="rho"
                     /locus_tag="VNP73_05920"
     CDS             complement(496..2094)
                     /gene="rho"
                     /locus_tag="VNP73_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWL65494.1"
                     /translation="MATVDRSVLESKGVAELKEIAKTLDIKTSGLKKAEIVDAIANGG
                     SKPADAKPREQAPASPEAKRESAPPAPSGNGVRDEHAVDDRDTRDDRRRGPQGRNNRN
                     RPREKAEPREGRGRRRRRGRGGTGGTGGSGGGGGGGGNVPHYMREDRWEDLEDIDDSD
                     AEVRTGILDLLPEGYGFLRTSGYLPGDKDVYVSVSQVRRFHLRKGDEITGSVRSPKES
                     EKYQALLKIREVNGVEVEAAQNRPRFADLVPLYPEERLTLEHPDNIKAITPRIIDLIA
                     PIGKGQRGMIVSPPKAGKTTVMKEIANSVRANNPETHLIVLLVDERPEEVTDMQRSVD
                     GEVIYSTFDRPSDDHIQVTELTLERAKRLVEMGKDVMILLDGITRLSRAYNLATPASG
                     RILSGGVDSTALYPPKRFFGAARNIEHGGSLTILATALVETGSRMDEVIFEEFKGTGN
                     WELKLDRKMADKRIFPAIDIPASGTRKEELLLTPEELQLAWRLRRVLHAVEGGALELL
                     IDKMKATQGNAQFLAEIAKAPVRE"
     gene            complement(2117..2797)
                     /gene="fsa"
                     /locus_tag="VNP73_05925"
     CDS             complement(2117..2797)
                     /gene="fsa"
                     /locus_tag="VNP73_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HWL65495.1"
                     /translation="MKLFLDTANLDEIREGARWGVLKGVTTNPTLIAKEEQGFLPLVK
                     EICSIVEGDVSIETASDDTEILVRQGHDIAQIADNAVVKIALTPNGLAATRRLADEDI
                     KVNVTLCFTAAQAVLAAEAGAYIISPFLGRLDDVATDGLRLLSDICEIYAVQGYDTQV
                     LAASLRHPVHVVEAAKMGSDIATMPFKVFESLVKHPLTDAGFERFLKDWAGAKPHLGE
                     LPPSYQEA"
     gene            complement(2781..3848)
                     /locus_tag="VNP73_05930"
     CDS             complement(2781..3848)
                     /locus_tag="VNP73_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65496.1"
                     /translation="MSEPRRKHVFIAEREHGLPYSKGLTASSLMATGLAPMRAYAVAN
                     KVEKKLLSLGRERVSREQLRELTLEVLRDEEGDEIAEDFIKWQSVAELDIPLIILIGG
                     ATGVGKSTIATQLAYRLGITRVVSTDAVREVLRSAFTQEMFPTLYTSSFDADEAVRQP
                     IPHSGDRLVIGFREQAAAVAVGAQRLIERAITEGTDMILEGAHLVPGFLETVESDQAV
                     IVSLLVTIEEEELHRSHFYRRGRDAGGRPEDRYLNAFKKIRKIQKYMTNSALVRSVPV
                     IPHYDLDATLSQIIDHVIAKALEAAERGAHVEGSDEKLLERAVEAVGKKSKGERGNGS
                     GASGAKGPSTEEGSKSREVVP"
     gene            complement(3849..5228)
                     /locus_tag="VNP73_05935"
     CDS             complement(3849..5228)
                     /locus_tag="VNP73_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HWL65497.1"
                     /translation="MEVHIRCLVIIDGEHYPPVIEAALREITDDGYDIEGALLIGGRE
                     KLPDEDLEEIGGVPIISGDDATRVLEDSIRRIRPELVIDLSDEPVLDYRGRHLLAGIA
                     LANQVRYQGADFVFEPPPRPRVATKPSIAVISTGKRTGKTAMAGFTARVLSDAGHSPV
                     IVAMGRGGPEEPEVLRGDQIELGPKELMAIANQGRHAASDYIEDALLGQVPTVGCRRC
                     GSGLAGGVDHSNVAEGVALANDLPGDLLILEGSGAAIPPVHADATIVVLPAHVDLEYL
                     RGYMGPYRLLLSDLAVVTLCEEPFGSPSRISEITSLVGESFRSQSLGDEGRGIRVVRT
                     VFRPAPTRPVDGKKAFVATTAPEEVGDSLKEHLEAEHGCEVVGMSHQLSDRKKLTREI
                     EKMSEHADLLLCEIKAAGVDVATRKALDEGLDVVYMNNVPQGVEGDDPSAALREVAEI
                     AVERFERSY"
     gene            5321..5938
                     /locus_tag="VNP73_05940"
     CDS             5321..5938
                     /locus_tag="VNP73_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65498.1"
                     /translation="MAKDATTRYRQLAFVRPARGGVSGGAIFTGVVVAFGSMFLLSAV
                     VAGVLATIGFDAADAGDSPIEAGLGAGAALVLAQFFSYLWGGYTAGRMGRGAGLANGL
                     LVPLIALLLAVVVAAVAAGLGTSANLNLPFQAYRLPVEDDVLVQWSVGIGIATLAAMF
                     LGGAVGGMLGTRWHTKLEAAAVAGASTPPEDATEVAGRGPKAENL"
     gene            5943..6380
                     /locus_tag="VNP73_05945"
     CDS             5943..6380
                     /locus_tag="VNP73_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWL65499.1"
                     /translation="MRKLVLAALTGLLVVGLMTPSASATVPVEWLESGGTLFVGGGTA
                     LSNGVFFPGTVIYDGDQFQGLPPLQLKKGTDLDFVNLDVSAVTNSHQIVSFKRKRGNP
                     VFSSESLDGPGTTVVITSHLKPGVYPFFCTTHFGMYGRLEIVP"
     gene            6488..>6723
                     /locus_tag="VNP73_05950"
     CDS             6488..>6723
                     /locus_tag="VNP73_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65500.1"
                     /translation="MDLERERRLWDLARTQFGVLTRGQALSLGYTPRMIQHRTECGAW
                     GVLHPAVYAVAGTPISWKRDQMAACLWAEGVSAGL"
BASE COUNT         1117 a         2158 c         2096 g         1352 t
ORIGIN      
        1 tccggggaag gtggagccgc cggtggcggc gagatcaggg tcgagctggg cggcgagcgc
       61 tgtcagcttg acgccgagca gccgggaccg gcgctcggcg tcgagggcgg gcaagcgccc
      121 ggcttacttc ttgtcgcgcc ggccgtagcg ccgctcgaac ttctcgatgc gaccgccgct
      181 gtcgaccagc ttctgcttgc ccgtgtagaa gggatggcac tggttgcaca gctcgacgtg
      241 gagctcggac ttcgtcgagc gggtcgtgaa cgtgttcccg cacgagcagg tcacgatcgt
      301 ttccatgtac tcggggtgta tgccttgctt cattcctgat cctcttgttc tctctgctct
      361 ctagcctgac gagctttctc gtaggcgacc gaggccctgg ccatgacctt gaaggtcacc
      421 cacaggcccc cgaccaaccc gatgataagc agccactcgg gaagacccaa acccaagaac
      481 atcagtggag ggccgctatt ccctcaccgg cgccttggcg atctcggcaa ggaactgagc
      541 attgccctga gttgccttca tcttgtcgat cagaagctcg agggcacctc cctcgaccgc
      601 gtggagaacc cggcgcaggc gccacgccag ctggagctct tcgggggtca gtagcagctc
      661 ttccttccga gtaccggagg cggggatgtc gatggcaggg aagatccgct tatcggccat
      721 cttgcgatcc agcttcagct cccagttacc cgttcccttg aactcctcga agatgacctc
      781 gtccatccgc gatcccgtct cgaccagagc cgtggcgagg atcgtcagtg agccgccgtg
      841 ctcgatattg cgagcggcac cgaagaagcg cttcggtggg taaagggcgg tcgagtccac
      901 accacccgac aggatccggc cggaggccgg cgtcgcgagg ttgtacgcac gcgacaggcg
      961 cgtgatcccg tcgagcagga tcatgacgtc tttgcccatc tcgacgagcc tcttggcgcg
     1021 ctcgagggtg agctcggtga cctggatgtg gtcgtctgaa ggccggtcga acgtcgagta
     1081 gatgacctcg ccatcaacgc ttcgctgcat gtcggtgacc tcttcaggtc gctcgtcgac
     1141 aagcaacacg atgagatggg tctctgggtt gttggcgcgg accgagttag cgatctcttt
     1201 catgaccgtc gtcttgccgg ccttcggtgg gctaacgatc atcccgcgct ggcccttacc
     1261 gatcggcgcg atcagatcga tgatccgcgg cgttatggcc ttgatgttgt cgggatgctc
     1321 gagcgtcagt cgctcttccg gatacagcgg cacaaggtcg gcgaaacgcg gacggttctg
     1381 cgccgcctcg acttcgaccc cattgacctc gcggatcttc agcaacgcct ggtacttctc
     1441 cgattccttc ggagatctga ccgaaccggt gatctcgtcg cccttgcgca gatggaaacg
     1501 gcggacctgc gacacagaca cgtacacgtc cttgtccccg gggaggtagc cactcgttct
     1561 caggaagccg tagccttccg gaagaagatc gaggatcccg gtgcgaacct cggcatccga
     1621 atcgtcgatg tcttcgagat cctcccagcg atcctctcgc atgtagtgcg ggacgttgcc
     1681 accgccgccg ccgccgccgc cactgccgcc agtgccgcca gtgccaccac gcccgcgccg
     1741 gcgacgtctc ccgcgacctt cacgcggctc tgctttctcg cgtgggcggt tcctgttgtt
     1801 gcgtccctgg gggccgcgtc tgcggtcgtc gcgcgtgtcg cgatcgtcca ccgcgtgctc
     1861 gtcgcgcaca ccgttgcccg aaggcgccgg tggagcgctc tcgcgcttgg cctccggtga
     1921 ggccggagcc tgctcgcggg gcttggcatc ggccggcttg gatccgccgt ttgcgatcgc
     1981 gtcgacgatc tctgccttct tcaaaccgga cgtcttgatg tccagcgtct tcgcgatctc
     2041 cttgagctcg gcgacacctt tggactcgag caccgagcga tctaccgttg ccacttaact
     2101 ctccctgcgg tcacttctat gcctcctgat atgagggtgg taattcacct agatggggtt
     2161 tggcgccggc ccagtccttg aggaacctct cgaaccctgc gtcggtcagc ggatgtttca
     2221 cgagagattc aaagaccttg aacggcatcg tcgcgatgtc tgatcccatc tttgccgcct
     2281 cgactacgtg aaccgggtga cgaaggctcg cggccagcac ctgggtgtcg taaccctgga
     2341 cggcgtagat ctcgcagatg tcggatagca acctgaggcc atccgtcgcg acatcgtcaa
     2401 gcctacccaa gaatggggaa atgatgtacg cacccgcctc tgcggccaaa acggcctgag
     2461 cggcggtgaa acagagcgtg acgttcactt tgatgtcttc atcggccagc cggcgagtgg
     2521 ccgccaggcc gttcggagta agagcgatct ttacgactgc gttgtccgcg atctgagcga
     2581 tgtcgtggcc ctgccgcacc agtatctcgg tgtcgtcgga ggcggtctcg atcgacacgt
     2641 ccccttccac tatcgagcag atctctttca cgagcggcag aaagccctgc tcttccttcg
     2701 caatcagtgt tgggttggtc gtcactccct tgagcacgcc ccaccgcgct ccctctctga
     2761 tctcgtcgag attcgccgta tcaaggaaca acttcacgtg acttgcttcc ctcctcggtc
     2821 gatggaccct tggctccact agctccgctg ccgttgccgc gctcgccctt gcttttcttc
     2881 cccaccgctt cgacggcgcg ctccaggagc ttctcgtccg acccttcgac atgggctccg
     2941 cgctctgccg cctcgagcgc tttcgcgatc acgtgatcga tgatctgaga gagcgttgca
     3001 tcaagatcgt agtgcgggat caccggaaca cttcgcacga gcgccgaatt cgtcatgtat
     3061 ttctgaatct ttctgatctt cttgaaggca ttgaggtagc gatcctcggg ccgtcccccg
     3121 gcgtcacggc cgcgacgata gaagtggctg cggtgaagct cctcttcctc gatggttacg
     3181 aggagggaaa cgatgactgc ctggtcactc tcgaccgtct cgaggaagcc cgggacgagg
     3241 tgggctcctt ccaagatcat gtccgttccc tcggtgatgg cgcgctcgat caatcgttgg
     3301 gcaccaacgg caaccgccgc tgcttgctcg cggaaaccga tcacgagccg atcgcccgag
     3361 tgaggtatcg gctggcgcac cgcctcgtcg gcgtcgaacg acgatgtgta aagggtcggg
     3421 aacatctcct gagtgaacgc gctgcgaaga acctcgcgaa ctgcgtccgt ggagacgact
     3481 cgcgtgatcc ccagacgata ggcaagctgg gttgcgatgg tggacttacc gacgccggtc
     3541 gcgccgccga tcaggatgat cagggggatg tcgagctcgg ccaccgactg ccatttgatg
     3601 aagtcctcgg cgatctcatc gccttcctca tcgcggagaa cctcgagcgt gagctcacgc
     3661 agttgctcgc gcgagacgcg ttcgcggccc aaggagagga gctttttctc gaccttgttg
     3721 gcgaccgcgt aggcgcgcat aggggccagt ccggtcgcca tgagagacga agcggttagt
     3781 cccttcgaat agggaaggcc gtgctcgcgc tcggcgatga agacgtgttt acggcgtggt
     3841 tcgctcacct agtaggacct ttcgaaacgt tcgacggcga tctcggctac ctctcttagg
     3901 gccgccgaag ggtcatcgcc ctcgacaccc tggggaacgt tgttcatgta gacgacgtcg
     3961 aggccctcat ccagagcctt gcgggtggcc acgtcgacac ctgcagcttt gatctcgcaa
     4021 aggagtagat cggcgtgttc gctcatcttc tctatctctc tggtgagttt tttccgatcg
     4081 gagagttgat gactcatccc caccacctcg cacccgtgct ccgcttcgag gtgctctttc
     4141 aaggaatccc ccacctcttc cggtgccgtc gtcgcaacaa aagctttctt gccgtcgacg
     4201 ggccttgtcg gtgcgggacg gaagacggtt cgaaccactc gaatgccccg gccctcgtct
     4261 ccgagtgact ggcttctgaa agattcgccg accagcgatg tgatctcgga gatccgggag
     4321 ggagaaccga acggttcttc gcaaagtgta accacggcca gatccgatag caatagtcgg
     4381 tacggaccca tatatccgcg caggtactcg agatccacgt gcgcggggag gaccacgatc
     4441 gtggcgtcgg catgcaccgg agggatcgcc gccccggagc cctccaggat gaggagatct
     4501 cccggaaggt cgttcgcgag ggccaccccc tcggcgacgt tcgagtgatc cacgcccccc
     4561 gcgaggccgc tcccgcacct cctacacccg acggtaggaa cctgtccgag caacgcatcc
     4621 tcgatgtaat cggaggccgc gtgacggccc tggttcgcga tggccatcag ttccttgggg
     4681 ccgagctcga tctggtcgcc ccgaagcacc tcgggctcct cgggtccccc ccgtcccatg
     4741 gcaacgatga ccggcgagtg ccccgcgtcg ctcaggacgc gcgccgtgaa cccggccatc
     4801 gccgtctttc cggtccgctt gccggttgag atcactgcga tcgacggttt cgtcgcgacc
     4861 cggggccggg gtggtggttc gaagacgaag tccgccccct ggtagcgaac ctggttcgcg
     4921 agcgcgatgc cggcaagcag atgccgtcct ctgtaatcca gcaccggctc gtcggagaga
     4981 tcgatgacca gctccggccg gatgcgacgg atcgaatcct cgagcacccg ggtggcgtcg
     5041 tccccggaga tgatcggaac gccaccgatc tcttccagat cctcgtcggg gagcttctcg
     5101 cggcccccga tcagaagggc cccctcgatg tcgtaaccgt catcggtgat ctcccgcaaa
     5161 gcagcttcga tcacgggtgg atagtgctca ccgtcgatga tgacgagaca gcggatgtgg
     5221 acctccaacg gtgcgaggaa cggggggatt gtggttcgat cttctggagc cgggtaactc
     5281 tatgcccgtt agcagttcga gctaagcatc gaggtgcacg atggccaagg acgcgaccac
     5341 ccgctaccgg caacttgcgt tcgtccgccc ggcgcgcgga ggtgtctctg gaggcgcgat
     5401 cttcaccgga gtcgtcgttg ccttcgggtc gatgtttctg ctgtccgcgg tcgtggcagg
     5461 ggtgctggcc accatcggtt tcgatgccgc cgacgcgggc gactcgccga tcgaagcggg
     5521 cctgggggcg ggggcggcac tcgtactcgc tcagttcttc tcgtacctgt ggggtggtta
     5581 caccgcgggc cggatgggcc gcggagcggg tctggccaac ggcctcctcg ttccgctgat
     5641 cgcgctactg ctggcggtcg tagtcgctgc ggtggccgcc ggcctcggca cctcggcgaa
     5701 tctcaacctg cccttccagg cgtaccggct gccggtcgag gacgacgtgc tggttcagtg
     5761 gagcgtcggg atcgggatag ccaccctcgc ggcgatgttt ctcggggggg ccgtcggggg
     5821 gatgcttgga acccgctggc acacgaagct ggaggcggcc gccgtcgccg gcgcctccac
     5881 gccgcctgag gacgccaccg aggtcgcagg gaggggcccg aaggcggaga atctctagac
     5941 atatgagaaa gctcgttctg gccgcgctca cgggtctgct cgtcgtcggc ctgatgaccc
     6001 cgtcggcgtc cgccacggtc ccggtcgaat ggctggagag cggtggcacg ctgttcgtcg
     6061 ggggcggcac ggctctctcc aacggggtct tcttccccgg caccgtgatc tacgacggcg
     6121 accagttcca gggcctgccc ccgttgcagc tcaagaaggg cacggatctc gacttcgtga
     6181 acctcgacgt atcggcggtg acgaacagcc accagatcgt gtcgttcaag cgcaagaggg
     6241 gaaaccctgt gttctcttcg gaatccctcg acgggccggg aacgacggtc gtcataacgt
     6301 cgcacctgaa gcccggcgtc tacccgttct tctgcacgac gcacttcggg atgtacggcc
     6361 gcctggagat cgtcccctag cccttcttgg ctaactgagt aacgttccag cgcgctgtga
     6421 cgcgacaagt tcgatcctgt gagcctggtc acggcaacga acccggatgt catgtgcact
     6481 ggttcccgtg gacctcgaac gtgagcgaag gttgtgggac ctagctcgta cccagttcgg
     6541 tgtgctcacc agaggccaag cgctttccct cggctacacc ccaaggatga tccagcaccg
     6601 aaccgaatgc ggtgcctggg gagtccttca tccggccgtt tatgcggtcg ctgggacgcc
     6661 gatctcatgg aaacgagatc agatggccgc ttgcctgtgg gccgagggtg tgtccgcagg
     6721 act
//
LOCUS       DATMED010000230         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1713857, whole genome shotgun sequence.
ACCESSION   DATMED010000230 DATMED010000000
VERSION     DATMED010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713857"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VNP73_05955"
     CDS             complement(<1..933)
                     /locus_tag="VNP73_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWL65501.1"
                     /translation="MSDISVAVTGPTGAIGRAFIRALEEDDSVTRVVGMARRPFDPAE
                     HGWKKFEYLQGDVTDVDSVTKLVAGADVVVHLAFLILGDADEAKKINVDGSRNVFEAT
                     FDAGAERLVYTSSVAAYGFHDDNPSLLDEDVPPRGSPEHYYSAQKAEVEGILHKMMQG
                     WPTDVYVFRPCIVAGPSATELIEEIPYVKIGSKLPKGLRAAVTGLPVLRPVIPDPGTP
                     FQLVHEEDVASALVAAVKGGVVPGAYNLAGDGEITATEIARALGWYAVPVPDLALDAT
                     AKIVSRLPYLPAQASWINAVRVPVLMDTSKARRNL"
     gene            complement(930..1454)
                     /locus_tag="VNP73_05960"
     CDS             complement(930..1454)
                     /locus_tag="VNP73_05960"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HWL65502.1"
                     /translation="MPDIVLVHGDITERPVDAIVNAANSSLMGGGGVDGAIHRKGGPA
                     ILAACKKVRADLYPDGLPAGDAVATTGGGLPARWVIHTVGPVWSSAEDRSGLLASCHV
                     SSLRIADEVGAAKVAFPAISTGVYGYPVDDAAIVAIGAVAEAATKVQEVEFVLFDDVA
                     YAAFETALERVGVT"
     gene            1528..2328
                     /locus_tag="VNP73_05965"
     CDS             1528..2328
                     /locus_tag="VNP73_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005116473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HWL65503.1"
                     /translation="MVDIDSLCTDLYDEHASLDEIVLSLDQEMWDRPTPAEPWSIRDQ
                     ISHLAFFDEQATLALLEPDRFNAGLQRAIEDLQGFMDAPLEMGRALPHEEVLGWWRGA
                     RTQMLKAFYEADRAARVPWFGPPMSLASFISARLMETWAHGQDVADALLIEREPTDRL
                     QHVCHLGVRARRNSYAAQGREFPGGDVRVELKAPSGEQWTLGESTEDIVRGPALDFCL
                     VVTQRRHLADTALQVEGDLAKEWMSIAQAFAGPPGPGRTPGQFRPPTT"
     gene            2409..2795
                     /locus_tag="VNP73_05970"
     CDS             2409..2795
                     /locus_tag="VNP73_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWL65504.1"
                     /translation="MRAVLDEARRGVLSTIAVDGSPHSVPVVFVIADEEIVTPIDDKP
                     KDGADLVRVHNIQRDPRATLLVDRWTEDWTRLGWVMVRGVMRIEEPSDFGPQLLQRYE
                     QYTDDMTPGSRALVLTPQRISWWRWS"
     gene            2780..3538
                     /locus_tag="VNP73_05975"
     CDS             2780..3538
                     /locus_tag="VNP73_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65505.1"
                     /translation="MALVVIDIDLAIEKVLSSGDEWQSWRALKLGGDDPGMMPAIPGQ
                     DGEGGFMGPSGTRPSPGATGEALCHFVVLGLDVSGPATVAADWLVEARTPAEAWLDSP
                     DDVPGELDDPAGGRVWATASASCGLLAVARDPGPRAISLLRGEADQSGHFTGGAYPTF
                     AAAGAYWLAEGPKTEMTEWALRWARERIDEDALEPWDLVGALIFWGAARIPLEHLSVD
                     RYIDALKAAAPDKGWPGDVELTLRTLEVLNFYEA"
     gene            complement(3544..>3674)
                     /locus_tag="VNP73_05980"
     CDS             complement(3544..>3674)
                     /locus_tag="VNP73_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HWL65506.1"
                     /translation="PCPVCGETLQRVSYESHEVAYCPNCQTQGKVLADRRLSRILK"
BASE COUNT          661 a         1188 c         1171 g          654 t
ORIGIN      
        1 caggttccta cgagctttgc tcgtgtccat cagtaccggc accctcaccg cattgatcca
       61 cgatgcttga gccggcaaat aggggagtcg ggacacgatc ttggccgtcg catccaacgc
      121 aaggtcgggg accgggaccg cgtaccaacc gagtgcgcgt gcgatctcgg tcgcagtgat
      181 ctctccgtcg ccggcgaggt tgtacgcgcc cggaacaaca ccacccttca ccgcggcgac
      241 gagagccgac gcgacatctt cttcgtgtac gagttggaac ggcgtgccgg ggtccgggat
      301 gacggggcgc agcaccggaa gacccgtgac cgcggccctc aatcccttgg ggagcttcga
      361 tccgatcttc acgtacggga tctcctcgat gagttcggtc gcgctgggtc cggccactat
      421 gcagggacgg aacacataaa cgtcggtggg ccagccctgc atcatcttgt gcaggatgcc
      481 ctcgacctcg gctttctgcg ccgagtagta gtgctccggc gagccgcgtg gaggaacgtc
      541 ctcatctaga agagacggat tgtcgtcgtg aaacccgtac gccgcgaccg acgacgtata
      601 gacgagtcgc tcggcccccg catcgaacgt cgcttcgaac acgttgcgcg acccatcgac
      661 attgatcttc ttagcttcgt cggcatcgcc gaggataagg aacgcaaggt gcaccacgac
      721 gtccgcgcct gccacgagtt tggtgaccga atcgacatcg gtcacgtctc cttgcaggta
      781 ttcgaacttc ttccaaccgt gctcggcggg atcgaaggga cggcgcgcca taccgaccac
      841 acgcgtgacc gagtcgtcct cttcaagagc cctgatgaac gctcgcccga tcgcccccgt
      901 tgggccggta accgctaccg agatatcgct catgtcacac ccacccgctc cagtgccgtt
      961 tcgaatgctg cgtaggcaac gtcgtcgaac aagacgaact caacctcctg caccttagtc
     1021 gcggcctccg caaccgcacc gatggcgacg atcgctgcat cgtccaccgg gtacccgtaa
     1081 acgccggtcg agatcgcggg gaaggcaacc ttcgcggccc ccacctcgtc ggcgatccgc
     1141 aaagaggaca catggcagga ggcgagtagg cccgaacgat cttcggccga gctccacacc
     1201 ggtcccaccg tgtggatgac ccaccgagcc ggcaaccctc ctccggtcgt ggcaaccgcg
     1261 tcccctgcag gaagcccgtc ggggtagagg tcggccctca ccttcttgca cgccgcgagg
     1321 atggccgggc cccccttgcg gtggatcgca ccgtcgacac cgccaccgcc cataagagag
     1381 gagttggccg cgttcacgat cgcatcgacg ggccgctcgg tgatgtcgcc gtggaccaga
     1441 acaatgtcgg gcacggtcat cacggtacta gcgttcctcc gtctgcgcga tggccgagcc
     1501 catcgcttct cctgctagct tttcgacgtg gtcgatatcg attccctttg cacagacctt
     1561 tacgacgaac atgcgtccct cgatgagatc gtcctgtcgc tcgaccagga gatgtgggat
     1621 cgcccgacgc ccgccgagcc gtggtcgatc cgggatcaga tcagtcatct cgcgttcttc
     1681 gacgagcagg cgaccttggc cctgctcgag ccggatcgtt tcaacgccgg actccaacga
     1741 gcgatcgagg acctccaggg cttcatggac gcaccgctcg agatgggccg ggcgttgccg
     1801 cacgaggagg tactcggctg gtggcgcggc gctcgcactc agatgctcaa agcgttctac
     1861 gaggccgatc gggccgctcg cgtcccctgg ttcggccccc ccatgagcct ggcgtccttc
     1921 ataagcgcgc gcttgatgga gacgtgggcc cacggtcaag acgtcgccga cgctcttctg
     1981 atcgaacgcg agccgaccga ccgcctccaa catgtttgtc acctcggagt aagagcgagg
     2041 cgcaatagct acgccgcgca gggacgtgag ttccccggcg gtgacgtgcg ggtcgaactc
     2101 aaggcgcctt cgggcgagca gtggaccttg ggggagtcaa cagaagacat cgttagagga
     2161 ccggctctcg acttctgcct cgtagtcacc caacgccggc acctggcaga cacggccctc
     2221 caagtcgagg gcgacctcgc gaaggaatgg atgtcgatcg cccaagcctt cgcaggtcca
     2281 cccgggcccg gacgaacgcc gggtcagttc cgacctccga cgacctagtg gtccgtacat
     2341 tgatgggctg gcacgcttaa tccgtggcga cagggtcgtg tcgatctctc tcggaacttc
     2401 cggccgagat gcgcgcggtc ctggacgaag caaggagggg agtcctatcg acgatcgcgg
     2461 tcgacggctc acctcattcc gtccccgtcg tcttcgtgat agcggacgag gagatcgtca
     2521 ctcccatcga tgacaagccg aaggacgggg cggacctggt tcgcgtgcac aacattcagc
     2581 gtgatcctcg cgccacgctg ttggtagacc ggtggaccga ggactggacc aggcttggat
     2641 gggtgatggt gcggggcgtg atgaggatcg aagagccgag tgatttcgga ccgcagttac
     2701 tacagcgcta cgagcaatac acagacgaca tgactccggg gagtagggca ttggttctga
     2761 ccccacaacg catctcgtgg tggcgttggt cgtgatcgat atcgacctcg caatcgagaa
     2821 ggtgttgtcg tccggggacg agtggcaatc gtggcgcgca ctcaaactcg gtggagacga
     2881 tcccgggatg atgccggcga tccccggcca ggacggcgag ggcggattca tgggtccgtc
     2941 cggaacgcgt ccctcgccgg gtgcgacggg cgaagcgttg tgtcacttcg tcgtgttggg
     3001 gctcgacgtt tccggtccgg cgacggtggc tgccgattgg ctcgtcgagg cgcgtacgcc
     3061 ggccgaagcc tggctcgact ctcccgacga cgttcccggc gagctcgacg atcctgccgg
     3121 cggcagggtg tgggcgaccg cgtcggcgag ttgtgggttg ctggcggtcg cgcgcgatcc
     3181 cggcccccgg gcaatctccc tactgagggg ggaggcggat cagtccggtc acttcaccgg
     3241 gggcgcgtac ccgacgttcg ccgctgccgg cgcgtactgg ctcgcggaag gaccgaagac
     3301 cgagatgacg gaatgggcgc tgcggtgggc acgcgagcgg atcgacgaag acgcgctcga
     3361 accatgggac ctcgtcggtg cactcatctt ctggggcgcg gcccgcatcc ccctcgaaca
     3421 tctgtctgtg gatcggtaca tcgatgcact gaaggccgcg gcccccgaca agggatggcc
     3481 gggagacgtt gagctcactc ttcgaacgct cgaggtccta aacttctacg aggcctaagt
     3541 tccttactta aggatccggg acaagcggcg gtcggccagc acctttccct gggtctgaca
     3601 gttcgggcag tacgcgacct cgtgactctc gtaggagacg cgctgcaacg tctctccgca
     3661 caccggacac ggca
//
LOCUS       DATMED010000231         4905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4784847, whole genome shotgun sequence.
ACCESSION   DATMED010000231 DATMED010000000
VERSION     DATMED010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4784847"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1533
                     /locus_tag="VNP73_05985"
     CDS             160..1533
                     /locus_tag="VNP73_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWL65507.1"
                     /translation="MAERLVVIGGDAAGMTAASQARKRRGPEDLSIVAFEKGNYTSYS
                     ACGIPYFVGDVVEFDRLIVRRPEEFRAKQDIDARIRHEVVGIDLDKRTVTVVHEGAES
                     DEPFDHLMVATGGKPLRPQLDGVDSTGIFGVQTLDDGVTIERFIEEEKPEKAVVVGGG
                     YIGLEMAEAFQLRGLKVALVERGPQPMATMDPDMGALVADAMRGLGVELLLEESVTGF
                     ETKDGKVSAVVTDARTLPADIVVLGLGTRPNVELAERSGIPIGESGGIVVDQRMKTPV
                     DGVWSAGDCAEKKHLVSDRPIAIALGTHANKEGRVAGINIGGGNATFPGVLGTATSKV
                     CGYEVARTGLRESDAEQVGFEVIGVRTDSTTRAGYYPGAAPITTKLVVERGSGRLLGA
                     QIFGKEGAAKRIDVLAMALWNGMTVDTILNVDLSYAPPFAPVWDPVLIAARRAWDQVQ
                     AEAAELR"
     gene            1530..2309
                     /locus_tag="VNP73_05990"
     CDS             1530..2309
                     /locus_tag="VNP73_05990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWL65508.1"
                     /translation="MSGLDELLSLAEEGALRAGETLMEHFIGPASGVDHKTTETDLVS
                     DADRAAESFLLDFISSKRPQDGVFAEEGGRENSESGLTWVVDPLDGTINFLFRIPHWC
                     VSVGVEDENGGVVGVIRNPNLDETFRAARDGGATLNSEPIRVSERRELATALIGTGFA
                     YDSEARRVQAEQVSRILPRARDIRRAGSAALDLAFLACGRLDGFYEASMERWDKSAGV
                     LIATEAGATITELPPPKEGLSPGVIGANEVLHRALVELLLA"
     gene            2314..2970
                     /locus_tag="VNP73_05995"
     CDS             2314..2970
                     /locus_tag="VNP73_05995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWL65509.1"
                     /translation="MAREIKIVDPAIERYMAELAAVDDEPVLLEMETVAKENGFPIIG
                     RLCGRMLEVLARSINAKRIFELGSGYGYSAYWFSRATGPDGEIHLTDGDPDNEGKAMD
                     YLKRAGLDGPIRFHVGDALTHLGQTEGEFDIVYCDIDKGEYADAWRKGRERVRVGGFY
                     IVDNMLWSGRVTGETEDNTPEGWTEAIMETNRLVAEDPAFRETIVPTRDGVLVALRIE
                     "
     gene            3004..>4905
                     /locus_tag="VNP73_06000"
     CDS             3004..>4905
                     /locus_tag="VNP73_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65510.1"
                     /translation="MGRHGGRGSPRAHGITPPETPERPARPGTFRRIIKTFRPYKKKV
                     TLVAVLILVTSGLGLINPLMIKSAFEALLAYDNPGEDPSELLRSLLIIVAIMVVTPII
                     SGGIGLWQTYTNTIVGQNVMQDLRNSLYAHLQHMPLKFFTSTRTGEIQSRLSNDVEGV
                     QGVLTDTASSIFSNVAIAISTVVVMTILSWQLTVLSLAILPFFMWLSRRVGKVRRDVS
                     GQTQEQLADLTAHMQETLSVSGILLSKAFGRQGYEIERFRRRNLRLSELEVRRSIIGR
                     GFFALIQIFFSITPAFVYLVAGWLLFRGSLGDSPEELVATIAAFTTLQSRLFFPMGQL
                     LNVNVEIQSALALFDRIFEYLDLEHEIVDKDGAIELAPSEVTGEVAFKDVWFRYDRPL
                     STEGEDARYWTIGGLSFTASPGELVALVGPSGAGKTTLTYLIPRLYDVGRGAVEIDGI
                     DVRDIRLASLGEVIGMVTQETYLFNASIRENLSYGNPDATDEQLEAAARAAHIYDRIA
                     EMPEGFDTVVGERGYKMSGGEKQRLAIARVILKDPRLLVLDEATSALDTISERLVQEA
                     LAPLMQGRTTIAIAHRLSTILAADTILYLDKGRILERGSHQELLAKGDAYAALYHEQF
                     SDGQVETRCE"
BASE COUNT          913 a         1427 c         1648 g          917 t
ORIGIN      
        1 cgttcagttt cagtcgaagg gagatgtctt cgcgcgtgca gcgctcgggt tgggcggatg
       61 cctcgccggg cacgaggacc acggcaagag caaggcaggc tagagctcgt cggtatttca
      121 tctatagacg ttagcccagg cgcggctagt ttcgtggcca tggcagagcg attggtggtg
      181 atcggagggg acgcggcggg catgaccgcg gcctcgcagg cccgtaagcg acgtggacca
      241 gaagatcttt cgatcgttgc tttcgagaag ggcaactaca cctcctactc cgcgtgcggg
      301 atcccgtact tcgtcggcga cgtggtcgag ttcgacagat tgatcgtgcg ccgcccggag
      361 gagttccggg ccaaacagga catcgatgcg cgcatccgac acgaggttgt tgggatcgat
      421 ctcgacaagc gcacggtgac ggtggttcac gagggagcag agagcgatga accgtttgac
      481 cacttgatgg ttgccaccgg agggaaaccg ttgcgccccc aacttgacgg ggttgactcg
      541 accgggatct tcggggttca gacgctcgat gatggtgtga cgatcgagcg ctttatcgaa
      601 gaggagaagc cggagaaggc ggtcgtcgta ggaggcggat acatcggcct cgagatggcg
      661 gaagcgttcc agctacgcgg tctgaaggta gcgctggtag agcgcggccc ccagccgatg
      721 gcgacgatgg atccggacat gggggctctc gtcgccgatg cgatgcgggg actcggtgtc
      781 gagctcctcc tggaggaatc ggttaccggc ttcgagacga aggacgggaa ggtgtctgcg
      841 gtggtgacgg atgcgaggac gcttccggcg gacatcgtgg tgctgggttt gggaacccgg
      901 cccaacgtcg agcttgccga acggtcgggg atcccgatcg gggagtctgg gggcatcgtt
      961 gttgatcaac ggatgaaaac tcctgtcgac ggcgtgtggt cggccggcga ttgtgcggag
     1021 aagaagcatc tcgtttctga ccgccccatc gcgatcgcgc tcgggactca cgcgaacaag
     1081 gagggacggg tcgccgggat caacatcggc ggcggaaacg cgacctttcc cggtgttctg
     1141 gggacggcga cttctaaggt ctgcggttac gaggtagcgc gcacgggtct ccgggaatcc
     1201 gacgccgagc aagtcggctt cgaggtgatc ggggtacgca ccgactcaac aacgcgcgcc
     1261 ggctactacc ccggcgcggc tccgatcacc accaagctgg tggtcgagcg aggttccggc
     1321 cggcttctcg gcgcacagat cttcggcaaa gagggggccg cgaagcggat cgacgtcctc
     1381 gcgatggccc tatggaacgg gatgaccgtc gacacgatat tgaacgtcga cctttcgtac
     1441 gcgccgccgt tcgcgccggt ctgggatccg gtgttgatag ccgcgcgcag ggcgtgggat
     1501 caggtccagg ccgaggcggc cgaacttcga tgagcggact ggatgaactg ctctccctcg
     1561 ccgaagaagg agcgcttcga gcgggcgaga ccctgatgga gcatttcata ggcccggcca
     1621 gcggtgtcga ccacaagacg accgagaccg acctcgtcag cgacgccgac cgcgcggcag
     1681 agtccttcct cctcgatttc atctcatcga agcgaccaca ggatggcgtg tttgcggaag
     1741 aagggggccg ggagaactcc gagtctgggt tgacgtgggt ggtggatccg ttggacggca
     1801 ccatcaactt cctgtttcgg atcccacatt ggtgcgtgag cgttggggtc gaggacgaga
     1861 acggcggagt ggtcggggtc atccgcaatc ccaatctcga cgagactttc cgcgccgcgc
     1921 gtgatggggg tgcaactctt aacagcgagc cgatccgggt cagcgaacgg agggagctgg
     1981 ccacagctct catcggaacc gggttcgcct acgactccga agctcgaagg gtccaggccg
     2041 aacaggtatc gaggatcctg ccgcgagcgc gtgacatacg cagggcaggg tctgccgctc
     2101 tggatcttgc cttcctggca tgtggacgct tggacggttt ctacgaagcc tcgatggagc
     2161 ggtgggacaa gtcggccggg gtcctgatcg ctacggaggc cggggcgacg atcaccgaac
     2221 ttccgccacc gaaagaggga ctgtcgccgg gggttatcgg cgcgaacgag gtcctgcatc
     2281 gggccctcgt cgagttgcta ctagcctagg cacatggcac gcgagatcaa gatcgtcgac
     2341 cctgcgatcg agcgttacat ggccgaactt gctgccgtgg acgacgaacc cgtgctgctc
     2401 gagatggaga ccgtcgcaaa ggagaacggt ttcccgatca tcggtcgtct gtgcggccgc
     2461 atgctggaag tgctggcgcg ttcgatcaac gcgaagcgga tattcgagct cgggtcggga
     2521 tacggatact ccgcttattg gttctcgcgc gcgactggtc cggacggaga gatccatctc
     2581 acggatggcg atcccgacaa cgaaggcaag gcgatggact acctcaagcg agcgggcctc
     2641 gacggtccga tccggttcca cgtcggagac gctctgactc acttgggcca aaccgagggt
     2701 gagttcgaca tcgtctactg cgatatcgac aagggcgagt acgcggatgc gtggcgtaaa
     2761 ggtcgcgagc gcgtccgggt cggcggcttc tacatcgtcg acaacatgct ctggtccggt
     2821 cgggtgaccg gcgagacaga ggacaacact cctgaggggt ggaccgaggc catcatggaa
     2881 accaaccggc tggtggccga ggatccggcg ttcagggaaa cgatcgtccc gaccagggac
     2941 ggggtcctgg tggcgctacg gatcgagtga aatacccgcc cccttcagat tgttctgcct
     3001 gacgtgggac gacatggagg ccggggctcg ccgcgagcgc acggcataac gccgccggag
     3061 acgcccgagc gtcccgcgcg gccggggacc ttccgccgga tcatcaaaac cttccggccg
     3121 tacaagaaga aggtcaccct cgtagcggtc ttgatcctcg tcacttcggg gttgggcttg
     3181 atcaaccccc tgatgatcaa gtccgccttc gaagccctgc tggcatacga caatcctggg
     3241 gaagacccct cggaattact tcgcagcctt ttgatcatcg tcgcgatcat ggtcgtgact
     3301 ccgatcatca gcggtgggat cggtctgtgg cagacgtaca cgaacacgat cgtcgggcag
     3361 aacgtgatgc aggacctgcg taactccctc tacgcgcacc tgcagcacat gcctctgaag
     3421 ttcttcacct cgacccgaac gggagagatc cagtctcggc tttcgaacga tgtggaaggc
     3481 gtccaaggag tcctgaccga tacggcgtcg agcatcttct ccaacgttgc catcgcgatc
     3541 tcgaccgtcg tcgtgatgac gatcctgagc tggcagctca ccgtgctgtc tttggcgatc
     3601 ctgccttttt tcatgtggct gtcgcgacgg gttgggaagg ttcgccgcga tgtttcgggt
     3661 cagacccagg agcaactcgc cgatctgacg gctcacatgc aagagactct gtcggtatcc
     3721 gggatccttc tgtctaaggc gttcggcagg cagggctacg agatcgagcg gttccgcagg
     3781 cggaacctca gactgtcgga gctcgaggtg cgccggtcga tcatcggtcg tggcttcttc
     3841 gctcttatcc agatcttctt ctcgatcacc ccggcattcg tctacctcgt cgccggatgg
     3901 ctgttgttcc gcggctcgct gggtgactcg ccggaggagc tggttgcgac catcgcggcg
     3961 ttcacgacgc tgcagtcgcg cttgttcttc ccgatgggac aactgttgaa cgtgaacgtc
     4021 gagatacaga gcgcgctggc cctcttcgac cggatcttcg aatacctcga cctcgagcac
     4081 gagatcgtcg acaaagatgg cgcgatcgag ctggccccgt cggaggtcac gggagaggtc
     4141 gcgttcaaag acgtctggtt ccgctacgac cgcccccttt cgacggaagg cgaggacgcg
     4201 cgctactgga cgatcggtgg tttgagcttc accgccagtc ccggggagct tgttgctctt
     4261 gtcgggccgt cgggcgccgg caagacgacg ctcacttatc tgatcccgcg cttgtacgac
     4321 gtgggccgtg gtgcagtcga gatagatggg atcgatgtac gtgacatccg tctcgcctcg
     4381 ctgggtgagg tcatcgggat ggtcacgcag gagacgtacc tgttcaacgc ctccatccgc
     4441 gagaacctta gttacgggaa ccccgacgcg accgacgagc agctggaggc ggcggcccgt
     4501 gctgcccata tctacgaccg gatcgcggag atgccggaag gcttcgacac ggtcgtcggc
     4561 gagcgtgggt acaagatgtc cggtggcgag aagcagcgtc tagcgatcgc ccgcgtcatc
     4621 ctcaaggatc cgcgcctctt ggtgctggat gaggcgacga gtgcgctcga cacgatctcg
     4681 gagcgtctgg tgcaggaagc gttggctccc ctgatgcagg gacggacgac catcgccatc
     4741 gctcaccgtc tctcgacgat cctcgccgcg gacacgatcc tctatctgga caaaggccgg
     4801 atcctcgaac ggggttcaca ccaggaactc ctcgccaaag gcgacgccta cgccgcgctg
     4861 taccacgagc agttctccga tgggcaggtg gagacgagat gcgag
//
LOCUS       DATMED010000232         3630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1330107, whole genome shotgun sequence.
ACCESSION   DATMED010000232 DATMED010000000
VERSION     DATMED010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330107"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VNP73_06005"
     CDS             complement(<1..555)
                     /locus_tag="VNP73_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWL65511.1"
                     /translation="MSVRTIEILGVQVASGTTPEALADVEGLFIRTTPALVAYVNAHT
                     LNLAYRDAEYRGVLQAADLVLNDGIGVKLAARLHGHRLPENLNGSDLNPAILGLSAQK
                     GWPVFFLGARPGVAKDVAEIMKTRWSGLKVAGCRDGFFTDEDEVVAEIRGSGAGLLMV
                     ALGNPMQEMFLARRLADTGAALGIG"
     gene            complement(546..2153)
                     /locus_tag="VNP73_06010"
     CDS             complement(546..2153)
                     /locus_tag="VNP73_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65512.1"
                     /translation="MITRLAALAAVLLLVAACEPEGAEEVGGASTPAARPTEARCDQL
                     PRLVERVERGIVRGLSPDVNLVPREPNYIGAAAAPVHSGPWDYLARVPLVMYGPGFVE
                     PGRYKGSADMSDVAPTTARLIGFDFSERDGRVLQQPLVRTTNHPRVVVTVVWDGGGIN
                     VLEEHPDSYPFLGSLLRKSATWPDMQIGSSPSVTPPIHATLSTGSYPKHHGIPGLRIR
                     TPDGEYVDPFIGLDPSNMRLPTLADAYDQARDNEPVVGMLAAVNWHLSMIGQGAGRPG
                     GDRDLAVLFDENGDVFSNEALFMLPDIGDTSYLERAAADLDASDGQRDGAWKGNDLSK
                     PLLRYSSPAHVAYQGFLLDRLLEVGNFGRDEIPDLLYVNFKPSDDAGHEWGMTSPEVG
                     DTIRAQDEELERLVENLDRTVGPRRWVLILTADHGQTPYPRESGAWPIGGGELTRDAN
                     AALDETDDDIGVVDRVSSPGAYVNMDQLRANELTVGDVARWMSSYTVADNVKEDQEMP
                     SSFEGREDELLFDAAVAEGRTFSEACR"
     gene            2211..3443
                     /locus_tag="VNP73_06015"
     CDS             2211..3443
                     /locus_tag="VNP73_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWL65513.1"
                     /translation="MAAPNVLLLVIDSLRADAIFGDRIATPNIQAFTDGGAAFTQCVS
                     TTTSTTPSFSSILTGCYPPKHGVRGLQGYSLNPAVTTMAEAFQRAGFQTHAEVTGPLL
                     PQTGILRGFEDANHRQGYKVPFFDWRERIVEKMHSYVSPWFLLLHIWEVHRPYRAPPD
                     FVKRWDRAGYEAAVAATDEWLAPVFDACGDNTITVITGDHGEDYPENPRQQKMIRAAR
                     KARRTFKLGSWFPYLDKRFASLAVGHGFALHEHLVRVPLIVSGPGIPQGRVEQQVRHV
                     DLFPTLAEICNLEDPGADGRSVLPLMKGQALPDEPAYIEAVGVKLEGNRIAGARTPDW
                     KLLKPGGGKPALYRLDGGAPPDEKRNLYSRYPEVARNLETFIDSVAASEVVSTTGSGM
                     TEAEEAVVEQHLRDLGYL"
     gene            3446..>3630
                     /locus_tag="VNP73_06020"
     CDS             3446..>3630
                     /locus_tag="VNP73_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWL65514.1"
                     /translation="MPGPPFFIVGSARSGTTLLRLMLNAHRSVAVPPESRFVVELWQG
                     HNEVEVNRFLSRLDAHKR"
BASE COUNT          677 a         1201 c         1101 g          651 t
ORIGIN      
        1 gccgatcccc agagccgcgc ctgtgtccgc gagccgccgg gccaggaaca tctcttgcat
       61 cgggttgccc agcgcgacca tcaacaggcc tgctcccgaa cccctgatct cggctacgac
      121 ctcgtcctcg tcggtgaaga agccatcgcg gcagccggca accttcaggc ccgaccagcg
      181 cgtcttcatg atctcggcga catctttcgc aacacccggc ctcgctccca gaaagaagac
      241 cggccaccct ttctgcgcgc tcaatcccag gatggccgga ttcagatcgg agccgttgag
      301 gttctccggc agcctgtggc cgtgcaagcg cgcggcgagt ttgacgccga tgccgtcgtt
      361 cagaaccagg tcggctgcct gcaggacgcc gcggtactcc gcatctcgat aagccagatt
      421 gagggtgtgc gcattcacat aggccacgag cgcgggggtc gtccgtatga acaaaccctc
      481 cacgtcggcc agagcctccg gagtcgtgcc tgaggcgacc tgcacgccga ggatctcgat
      541 cgttctcacc gacatgcctc cgagaaggtt cggccctccg cgacggccgc atcgaaaagc
      601 aactcgtcct cgcggccttc gaagctcgat ggcatctcct gatcctcctt gacgttatcg
      661 gccaccgtgt aggacgacat ccatctcgca acgtcgccga cggtcagctc gttcgcgcgc
      721 aactgatcca tgttgacgta cgcacccgga gaggagacac gatcgaccac accgatgtcg
      781 tcgtcggtct catccagagc ggcattcgcg tccctggtta gttcgccgcc cccgatcggc
      841 catgcgccgg actcgcgcgg atagggcgtt tggccgtgat cggccgtcag gatcagaacc
      901 catctcctcg ggccgaccgt acggtcgagg ttctcgacca ggcgctctaa ctcttcgtcc
      961 tgagcacgga tcgtgtcgcc gacctcgggt gaggtcatcc cccactcgtg cccggcgtcg
     1021 tcggacggtt tgaagttcac atacaggagg tccggaatct cgtcgcgtcc gaaattgccg
     1081 acctcgagca acctgtcgag aaggaatcct tgataggcga cgtgagccgg actcgagtac
     1141 ctaagcaatg gcttggacaa gtcgttgcct ttccaggcac cgtcgcgttg accgtccgag
     1201 gcgtcgagat ccgcggccgc ccgttccaga tacgacgtat ccccgatgtc gggaagcatg
     1261 aacagcgctt cgttcgagaa cacgtcgccg ttctcgtcga aaagcaccgc gaggtcgcgg
     1321 tccccacccg gccggcccgc tccctgtccg atcatcgaca gatgccagtt gaccgcagca
     1381 agcatgccga cgaccggctc gttgtccctg gcttggtcat aggcatccgc caaggtcggc
     1441 aatctcatat tcgatggatc caatccgatg aacgggtcga cgtactcgcc gtccggggtg
     1501 cggatgcgca gaccggggat cccatgatgc ttcggatagg aaccggtcga aagggtcgcg
     1561 tggatcggtg gggtcacaga cggagacgag ccgatctgca tgtccggcca cgttgccgac
     1621 ttccgcaaga gcgatcccag gaacggatac gaatcgggat gctcctcgag aacgttgatg
     1681 ccgccaccgt cccacacgac ggtgacgacc acgcgtggat ggttcgttgt tcggaccagg
     1741 ggctgctgca atacgcgtcc atctcgttcc gagaagtcga agccgatcag tcgcgcagtt
     1801 gtgggggcaa cgtcactcat gtccgccgat cccttgtatc tcccgggctc tacgaatccg
     1861 ggcccgtaca tgacgagcgg gacgcgagca aggtaatccc acggccctga gtgaacgggg
     1921 gccgcagcag caccgatgta gttgggctct ctcggtacga gattcacatc gggagaaagc
     1981 ccgcgcacga tcccacgctc gacccgttcg acaaggcgcg gcagttggtc gcagcgcgcc
     2041 tcggtcggac gcgccgctgg ggtggaagcg ccccccacct cttccgctcc ttcaggttcg
     2101 cacgccgcaa ccaggagcaa cacagcggcc agcgccgcaa gtcgagtgat catgagccgc
     2161 cggtaggata agtctgtccg tagacggttc ccccgacgtg gaggtcttgc ttggccgcgc
     2221 cgaacgttct gttgctggtg atcgattcgc ttcgagcgga tgcgatcttc ggcgaccgca
     2281 tcgcaactcc gaacatccag gcgttcacgg acgggggcgc ggccttcacc caatgcgtgt
     2341 cgacgacgac ttcgacgacg ccctcgttct cttccatcct gaccggctgc tatccgccca
     2401 aacacggcgt ccgcggctta cagggttatt cgttgaatcc ggcggtaacg acgatggcgg
     2461 aagcgttcca gcgagccggg ttccaaactc acgccgaggt caccgggcca ctgctgcccc
     2521 agacgggcat cctgcggggg ttcgaagacg cgaaccaccg gcagggttac aaggtgccgt
     2581 tcttcgactg gcgcgagcgc atcgtcgaaa agatgcattc gtacgtgtcg ccgtggttct
     2641 tgctcctaca catctgggag gtccaccgtc cctaccgagc accccccgac ttcgttaaga
     2701 ggtgggaccg tgccggttac gaagcggccg tggcggccac cgacgagtgg ctggctccgg
     2761 tcttcgacgc gtgcggtgac aacacgatca ccgtgatcac cggcgaccac ggtgaggact
     2821 atccggagaa cccgcgccag cagaagatga tccgcgcggc caggaaggca cgacgcactt
     2881 tcaagctcgg ctcctggttc ccatacctcg acaagaggtt cgcctcactc gcggtaggac
     2941 acggattcgc cctgcacgaa catctggtgc gggtgccctt gatcgtgtcg ggaccgggga
     3001 taccccaggg acgcgtcgag caacaggtcc gccacgttga cctcttcccg acattggccg
     3061 agatctgcaa tctcgaggat ccgggtgccg atggccggag cgtgctcccg ctgatgaaag
     3121 ggcaagcgct gccggacgag ccggcataca tcgaagccgt gggcgtcaag ctcgaaggga
     3181 accggatcgc cggtgcacgc acacccgact ggaagctctt gaagcccggc ggtggcaagc
     3241 ccgcgctcta ccggctggat ggaggcgctc cccccgacga gaagaggaac ctgtacagcc
     3301 gctaccccga ggtagcgcgc aacctggaga cattcatcga ttccgtcgcc gcgtcggagg
     3361 tcgtctccac taccggatcg gggatgactg aagccgagga agcggtcgtc gagcaacatc
     3421 tgcgcgatct cggttacctg taggtatgcc cggtccccca ttcttcatag ttggatcggc
     3481 acgctcgggc acgacgctcc tgcgactgat gctcaatgct catcgttccg tcgccgtccc
     3541 gcccgaatcc cgcttcgtcg tggaactgtg gcaaggacac aacgaagtcg aggtcaacag
     3601 attcctctct cgcctcgatg cacacaaacg
//
LOCUS       DATMED010000233         3949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2118905, whole genome shotgun sequence.
ACCESSION   DATMED010000233 DATMED010000000
VERSION     DATMED010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118905"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..550)
                     /locus_tag="VNP73_06025"
     CDS             complement(32..550)
                     /locus_tag="VNP73_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWL65515.1"
                     /translation="MTIADADVKDWVGKTLDAGPGDVVVEQGLIQHWCEATENGNPLF
                     WDDEVAEDLTGGPIAPPTMLSVWMRPLMWKPGPDDDSIRPLELHFQLKDAFDLPEGVV
                     ANNELEFHAPVRPGDLISTKQSVREISEVKETRLGTGRFWTIDVTYTNQRGETVGVES
                     YAMFSYRRENSP"
     gene            complement(550..1692)
                     /locus_tag="VNP73_06030"
     CDS             complement(550..1692)
                     /locus_tag="VNP73_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWL65516.1"
                     /translation="MTDEQLSGISVLDLSSVGPGSRCTALLADLGADVVKVGAPEGRI
                     EPPFFSYSAGRGTRRIAIDLRKPEGRDVFLRLARGADVIVESYRPGVADRLGVGYADA
                     SKVNERVVYCAITGYGQDGPYASWAGHDLNYLAVGGFLATQGRRADGGPAIPGATVAD
                     SAGGGMQAVIAILAAVLRRTVTNTGQYLDVSTTEGVLSLTSLYVDELLATGNETRPGS
                     NLLTGAFACYDLYRARDDRWLAVAAIEPKFFANLCRLLESERWIPHQMDPARQADIRD
                     AFAASFATRDAAEWVTLLGPADTCVAPVLTIGEVAQDPHLRARGLFAEAEHPERGRVP
                     QLASVIAGSERRDTYVAGSDASNEILQNAGMTAEEIEELRAQGVVV"
     gene            complement(1738..2322)
                     /locus_tag="VNP73_06035"
     CDS             complement(1738..2322)
                     /locus_tag="VNP73_06035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWL65517.1"
                     /translation="MDATPRIVGIGGTTRAGSSTEKALTYALDVCREEGATTVLFSAS
                     ELVGLPFYAPEHPERTPGAIRFVEEIRAADGLIIGSPGYHGGISGLIKNALDYTEDLR
                     EDGRPYLTGLPVGCIATGAGWQGTVSTLQALRDVVHALRGWPTPMGAALNTASPCFTP
                     DGQPSDEKVRFQLSTVANEVMSFTAWRGAGLLTG"
     gene            complement(2343..3557)
                     /locus_tag="VNP73_06040"
     CDS             complement(2343..3557)
                     /locus_tag="VNP73_06040"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWL65518.1"
                     /translation="MDQAVEAIRRGDIVIVADDPARENEGDFVMAAEKVTPEAVNFMV
                     THGRGVVCLPVTGERATELGLRPMVADCDEAMGTAFTISIDLREPRNTGTSAFDRAAC
                     ISKVTDPTARPEDFCSPGHVFPIQARDGGVLERAGHTEATVDLARLAGLAPAGVICEI
                     LKADGSMARMDDLEELARIHGLHLITVADLVAYRLQRETFVRREAQAQIPTVHGEFTA
                     IAFHSDLDGFDHLALVMGEPTGEDVLVRIHSECLTGDLGSLRCDCAAQLKESMRTIGD
                     AGRGIVVYLRGHEGRGIGITEKLRAYALQDQGADTVDANLQLGHSVDERCYDTAVQIL
                     RDLGASTIRLLTNNPAKCEALVAHGIPVVEQVPLQTTPTEENLRYLETKRDRLGHTLL
                     LDGPLVELLDPV"
     gene            3720..>3949
                     /locus_tag="VNP73_06045"
     CDS             3720..>3949
                     /locus_tag="VNP73_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009505958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL65519.1"
                     /translation="MDRQKGVFSVFDLSGRTAVVTGGGSGIGRATAEVLGGAGANVVL
                     GDIDEGAAEETVRSITEAGGKALSQRSDISSK"
BASE COUNT          666 a         1312 c         1299 g          672 t
ORIGIN      
        1 gccagaacct cggcggcagt gaacgcctcg gtcatggtga gttctccctc cggtaggaga
       61 acatcgcgta ggactccacg cccacggtct caccgcgctg gttcgtgtac gtcacgtcga
      121 tggtccagaa ccttcccgtg cccaagcggg tctccttcac ctcgctgatc tcgcggaccg
      181 attgcttggt gctgatcagg tcgccgggcc gcaccggagc gtggaactcg agctcgttgt
      241 tcgccacgac gccctccggg aggtcgaacg cgtccttgag ctggaagtgc aactcgagag
      301 ggcggatcga gtcgtcgtcc gggccgggct tccacatgag tggacgcatc cataccgaga
      361 gcatcgtggg cggcgcgatc ggcccccccg tcaggtcctc ggcgacctcg tcgtcccaga
      421 acaaggggtt gccgttctcg gtcgcctcgc accagtgttg gataaggccc tgctcgacga
      481 cgacgtcacc cggaccggca tcgagcgtct ttccgaccca gtccttcacg tcggcatccg
      541 cgatcgtcat cagacgacca ccccctgggc cctcagttcc tcgatctctt ccgcggtcat
      601 cccggcgttt tgaaggatct cgttcgacgc gtcggatccc gccacgtacg tgtcgcgccg
      661 ttcactgccg gcgatcaccg acgcaagttg cgggaccctg ccgcgctccg ggtgttcggc
      721 ctcggcgaac agcccgcgtg cgcggaggtg cggatcttgg gcaacctcgc caatggtcag
      781 aaccggagcc acacaagtgt cggcgggacc gagcagcgtt acccactcgg ccgcgtcgcg
      841 cgtggcgaac gaggccgcga acgcgtcgcg gatgtcagcc tgccgtgcag gatccatctg
      901 gtgcgggatc catctctcgc tctccagcag cctacagagg ttggcgaaga acttgggctc
      961 gatcgccgcg accgctaacc atcggtcgtc acgggcgcgg tacaggtcgt agcaggcgaa
     1021 cgcgccggtg agcaggttcg accccggccg ggtttcgttc ccggtggcca acagctcgtc
     1081 gacgtacagg gacgtaagcg acagcactcc ctcggtcgtc gagacgtcga ggtactggcc
     1141 cgtgttcgtc accgtccgac ggagtaccgc ggcgaggatg gcgatgaccg cctgcatccc
     1201 gccgcccgcg ctgtcggcga cggtcgcccc cgggatcgcc ggcccgccgt cggcgcgccg
     1261 accctgcgtc gcgagaaagc cgccgaccgc gaggtaattc agatcgtggc cggcccacga
     1321 tgcataaggt ccgtcctgcc cgtacccggt gatcgcgcaa tacacgacac gctcgttcac
     1381 cttgctcgcg tcggcgtaac ccacaccgag tcggtcggca acgcccgggc ggtagctctc
     1441 cacgatcacg tccgcgccgc gggcgaggcg aaggaatacg tctcgaccct ccggcttgcg
     1501 gagatcgatg gcgatccgac gagtaccgcg accggcgctg taggagaaga acggcggctc
     1561 gatccggcct tccggggccc cgaccttgac cacgtccgcg ccgaggtcgg cgagtaacgc
     1621 cgtacatcgg ctccccggac cgaccgagga gagatcgagt accgagatgc cggacagctg
     1681 ctcatcggtc atgcccggca gattatctga cggaccgtca gataacgatg tgggccgcta
     1741 tccagtgagg aggcctgcgc cgcgccacgc ggtgaacgac atcacctcgt tggccacggt
     1801 cgacaactgg aaccgaacct tctcgtctga tggctgcccg tcgggagtga agcaaggcga
     1861 cgccgtgttc agggcggccc ccatcggggt cggccacccg cgcagggcat ggaccacgtc
     1921 gcggagcgcc tggagtgtgg acaccgttcc ctgccagccc gccccggtgg cgatgcaccc
     1981 caccggcagc ccggtgaggt agggccggcc gtcttcacgg agatcctccg tgtaatcgag
     2041 ggcgttcttg atcaggcccg agatcccgcc gtggtacccc ggcgagccga tgatgagccc
     2101 atccgcggcc cggatctcct cgacgaagcg gatcgcgccg ggggtccttt cggggtgctc
     2161 tggcgcgtag aacggcagcc caacaagctc ggatgcggag aagagaacgg ttgtagcgcc
     2221 ttcctcacga cagacgtcaa gcgcgtacgt gagggccttc tccgtggacg atccggcgcg
     2281 ggtggtcccg ccgatcccca cgatccgtgg cgttgcgtcc atcaccatga cgtctcctct
     2341 cctcagaccg ggtcgagcag ctcgaccaac ggaccgtcga gcagcaaggt gtgaccgagt
     2401 cgatcgcgct tcgtctcgag atagcgaagg ttctcttcgg tgggagtcgt ctggagcggg
     2461 acctgctcca cgacggggat cccgtgggca acaagcgcct cgcacttcgc tgggttgttc
     2521 gtcagcaaac ggatcgtcga cgcgcccagg tcccgcagga tctgcacggc cgtgtcgtag
     2581 caccgctcat cgacagagtg acccagctga agattggcat ctaccgtgtc ggccccctgg
     2641 tcctgcagtg cataagcacg aagcttctcg gtgataccga tcccccggcc ttcgtgaccg
     2701 cggaggtaca ccacgatgcc tcgaccggcg tcaccgatgg tgcgcatcga ctccttcaat
     2761 tgagcggcgc aatcgcaccg caggctgccg agatctccgg tgagacactc ggagtgaatg
     2821 cgaaccagca cgtcctcccc cgtcggctcg cccatcacga gagccagatg gtcgaacccg
     2881 tcgaggtctg agtgaaacgc aatggcggtg aactcaccgt gcaccgtagg gatctgagcc
     2941 tgagcctctc gccgcacaaa tgtctcgcgc tggaggcggt acgcgacaag gtcggcgacg
     3001 gtgatcaggt gaaggccgtg gatccgggcc agttcctcga gatcgtccat ccgggccatc
     3061 gagccgtcgg ctttgaggat ctcgcagatc acaccggcgg gcgccaagcc ggccagtcgg
     3121 gcgaggtcga cggtcgcctc ggtgtggccc gctcgctcga gcacgccacc gtcacgggct
     3181 tggatcggaa acacgtgtcc cggagagcag aagtcttcgg gcctcgccgt cggatcagtc
     3241 accttggaga tgcatgcggc gcggtcgaac gcggacgttc cggtgttccg tggttcacgc
     3301 agatcgatgg agatcgtgaa cgcagttccc atggcctcgt cgcagtcggc gaccatgggg
     3361 cggaggccga gctcggtggc ccgctcgccg gtcacgggga ggcacacgac cccccggccg
     3421 tgcgtgacca tgaagttgac ggcctcgggg gtgaccttct ctgcggccat gacgaagtcg
     3481 ccttcgttct cccgtgccgg gtcgtcggcc acgatcacga tgtcgccgcg acggatggct
     3541 tcgacggcct ggtccaccgt ggaatgggtc gccgggcgac cgttcgtcgc tggcctactg
     3601 aatggcatgt gttcgatata tggaatcatt ggttgccttt gtaaacagta tcgaccttga
     3661 ggttcggcgt caaggagccc atccctgccc acagggcacc ggatatgctg ccaaacctga
     3721 tggaccgtca gaaaggcgtt ttctcggtat tcgacctctc aggacgcacc gccgtggtca
     3781 cgggcggagg cagtgggatc ggccgggcga ccgccgaggt tctcgggggg gccggggcaa
     3841 atgtggtcct gggcgacatc gatgaggggg ccgccgagga gaccgtccgg tcgatcaccg
     3901 aggccggtgg gaaggcgctc tcacagaggt ccgacatcag cagcaagaa
//
LOCUS       DATMED010000234         5590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_937499, whole genome shotgun sequence.
ACCESSION   DATMED010000234 DATMED010000000
VERSION     DATMED010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937499"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VNP73_06050"
     CDS             complement(<1..1423)
                     /locus_tag="VNP73_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL65520.1"
                     /translation="MPNQAAATGALPEIFQEAARLGGTLSILGDSTEETRSHREVADL
                     AALRAGGLADKGIGRGDRVCLFARTSFDFVADLLAVWHVGAVAVPLPIPPRFLATDNW
                     MDQAEAQIRNSRAACMLLAGDDPEPPSTKVVRAEDLEATGTYDGPGPTAEDVALIQWS
                     SGSTSRPKGVILTHRAIAAHLELNREHVLHFGPGPVFSWLPLYHDAGLLVYLLTPLSG
                     GATLTMLRTDSFLARPLRWLEEMDRTRAHVCCAPTFGYALAARALEGTEDTLDLSSWK
                     QAYIGAEPIDVRTVKRFLRAAEPHGFDARAMLPGYGLAEATCTVTVGVPMQGIRIDTI
                     DRSALAMGEAKPASRDTPGAADVVSVGVPAGDMEVIVVDTGDHELPDRGVGEVLVRGS
                     SLMEGYLKDAEATRAALANGWLHTGDRGYLADGELFITGRIKDVIVVRGRNHHAEDLE
                     QIVLALPDVRPGACVCIGIDDEGS"
     gene            complement(1444..3093)
                     /locus_tag="VNP73_06055"
     CDS             complement(1444..3093)
                     /locus_tag="VNP73_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL65521.1"
                     /translation="MSMPSYATTSTALPQVFNEGARLGNSLSIVGDSTEETRSHQEIV
                     EFAELRAGGLAEKGIRPGDRVALFAQTSFDFMADLLAVWRLGAVGVPLPIPPRFLAPD
                     AWMDQAVRRISDANAACVLLGAGDPEPLGVRPIWAVDLEDAGAYDGPLPAGEDVALIQ
                     FSSGSTSQPKGVVLNHRAVLTQIALAEEQRLLAGDGSRLGWVPLYHDLGLMGFMLPAL
                     GSGSTLTMMLTETFLTDPLRWLQEIANRGVQTSAAPTFGYALAARALERTDEMLDLSS
                     WKQAYVGAEPVDLRTTDRFLRAATKHGFDERSFIPCYGLAEAGCGVTSCGPQQGLQID
                     TVDRAALASGEAKPVPDDHPGVARFVSSGKPLVDFEVIVVDAADRELSERSVGEILVR
                     GSAVMDEYLLDAEASRATLAGGWLHTGDRGYLVGGELFVTGRIKDVIIVRGQNYYAED
                     IEQVVLAIPDVRPGGCASIGLAHEGARPLVVIAESDAAGDPVRSADVRDRIVRRIWSE
                     VGLRTKEVVLLPPRSLPKTSSGKLQRGLAKQMYLDGAWSDQ"
     gene            3107..4309
                     /locus_tag="VNP73_06060"
     CDS             3107..4309
                     /locus_tag="VNP73_06060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL65522.1"
                     /translation="MAGRLLRREHLRVLFVSPDYPPDVVGGIGPYTAEVAPQLADRGH
                     EVHVLCCRQGEHNEDETHGAVTVHRRGWRDTPGRGLITRAPEATLRWRNALETRRHIR
                     SLGHFDAIEVPDWKAFGAFLPRGIPLVLHLHMPLRVHAEYEPEFTDWDVTCSDLIERR
                     AVPKALVVTCPSNLLADRVRNGPWKIGTDIRIVRHAISPLPWQQAGPVATTPPTVLFV
                     GRLEPRKAPDLLIEGAAKLLERVPDLNVVFAGAETNTAKGLEFARSLRVSSERLGVPC
                     TFAGKQSREELVGWHARARLAVIPSRFDNFPMTGLEALAAGRPLVVSDQVGLAEIIGD
                     AGAVFPSGDPDALAAAMLPYLLDPVAAGRAGARAQVLARDNFSPQKIAAGRERCFAEA
                     AALVSSPT"
     gene            complement(4298..5248)
                     /locus_tag="VNP73_06065"
     CDS             complement(4298..5248)
                     /locus_tag="VNP73_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWL65523.1"
                     /translation="MTRLPLVTAIVPTRDRPASLHRALASVRSQTYPALEIVVVDDGS
                     TQPVEPEVVADHPPTRLVRLEPSRGPGAARNAGVEAASGELLAFLDDDDEWLPASVER
                     RVNALIEAGPSFAGAQCGWEMRESGDLRFSFVPDPDRDLQRTLLRRPSMAPSSVLIRK
                     SAFIEAGGFDASLSQYEDWDLWLRVADRYDVTLVPELLVRRELHSDRDLRAIRLYARQ
                     QMWERIGPRIERLDPLERRSLKAHHLAELAFIQSELGEYRAARRALAAAWLQQPWSLR
                     HATGIARSVLGNTSLWASAGKVKRVLRRNGGGVEPPVLSW"
     gene            5271..>5590
                     /locus_tag="VNP73_06070"
     CDS             5271..>5590
                     /locus_tag="VNP73_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65524.1"
                     /translation="MKILFAAHGNPSRTPGGIETYARCIAPALVDLGHEVHLLWLGFG
                     QGVHDGREGGVTVHLRPWRGPRALGRAVGLPHSWARIRVALAAKAALTQIGRRFDVIE
                     APEW"
BASE COUNT         1024 a         1859 c         1685 g         1022 t
ORIGIN      
        1 tggagccttc atcgtcgatg cctatacata cacacgcgcc gggacgcacg tctggaaggg
       61 ctagtacgat ctgctcaagg tcctcggcat ggtgattgcg gccgcgaacc acgatcacgt
      121 ccttgatccg acctgtgatg aacagctctc cgtcggcgag atagcccctg tcaccggtgt
      181 gcaaccagcc gttcgcgagc gcggcgcggg tcgcttcggc atccttcagg taaccctcca
      241 tgagagagct tcctcgaacc agcacctccc ccacccctcg gtcgggcaac tcgtggtcgc
      301 ccgtatctac gacgatcact tccatgtcgc cggccggcac gcccactgag acaacatcgg
      361 ccgcgccggg cgtgtcccgg gaggcaggct tcgcttcgcc catcgcgaga gccgaccgat
      421 cgatcgtatc tatccgtatc ccttgcatcg ggacccctac cgtgacggtg cacgtcgcct
      481 cggcaaggcc gtatcccgga agcatcgctc tggcgtcgaa accgtgaggt tctgcggcgc
      541 gcaagaaccg cttaacggtc cgcacatcaa taggctcggc cccgatgtag gcctgcttcc
      601 aagaagatag atcgagcgtg tcctcggtcc cttctaaagc ccgcgcggca agtgcatatc
      661 caaatgttgg ggcgcaacac acatgcgccc tagtcctgtc catctcctct agccatcgca
      721 acgggcgggc aagaaaggag tcggtgcgaa gcatcgtgag ggtcgcaccg ccggatagcg
      781 gggttagcag atacaccagc agacccgcgt cgtgatacag cggcaaccag gaaaacactg
      841 ggcctggacc aaagtgcaat acgtgctccc ggttgagctc caagtgagcc gcgatcgcgc
      901 ggtgcgtgag gatcactccc ttgggtcggc tcgtgctgcc cgaactccac tggatcagcg
      961 cgacgtcctc tgcggtcggt ccgggcccgt cataggtgcc cgtggcctcg aggtcttccg
     1021 ctcggacgac ctttgtactc ggcggctccg gatcgtcgcc ggccagcagc atgcacgccg
     1081 cgcgtgagtt tctgatctgc gcttcggctt gatccatcca gttatcggta gccagaaagc
     1141 gcggcggtat cgggagtggg acggcgaccg ctcctacgtg ccagaccgcc agaagatccg
     1201 ccacgaagtc gaacgatgtt cgggcgaaga ggcacactcg atcgcctcgg ccgatcccct
     1261 tgtctgcaag ccctccggcc ctgagcgccg ccagatcggc tacctctcga tggcttctcg
     1321 tttcctccgt cgagtctcca aggatcgaca gtgtgccccc aagacgagcc gcttcctgga
     1381 agatctcggg cagagccccg gtcgctgccg cctgattcgg catcctcttc ctctcgacct
     1441 gtttcactga tccgaccacg caccatcgag gtacatctgc ttggcgagtc ctctctgtag
     1501 cttcccgctg gacgtcttcg gcagagatcg gggcggtaga aggacgacct ctttcgttcg
     1561 aaggccgacc tccgaccaga tccgtctcac gatccggtcc cgaacgtcgg cgctccgaac
     1621 cggatcgccg gcggcgtccg attccgcgat tacaaccagg ggccgcgctc cttcgtgggc
     1681 aagcccgatg cttgcacaac caccgggacg gacatcggga atggcaagca ctacctgttc
     1741 gatgtcttcg gcgtagtagt tctggccccg gacgatgatc acgtctttga tccgcccggt
     1801 gacgaaaagc tcgccgccga cgaggtaccc gcggtccccc gtgtgtaacc atccgccggc
     1861 cagggtcgca cggcttgcct ctgcatccag cagatactcg tccatgaccg cactgccccg
     1921 caccaggatc tcgcccaccg atcgctccga caactcgcga tccgcggcat ccacgacgat
     1981 cacttcgaaa tcgaccaggg gcttcccgga cgagacgaac cgcgcaactc cggggtggtc
     2041 gtcgggaacc ggcttggctt cgcccgacgc gagggccgcg cgatcaacgg tatctatctg
     2101 taacccttgc tgagggccgc aagacgtcac tccgcacccc gcctcggcca atccgtagca
     2161 agggataaat gaacgctcgt cgaaaccgtg tttggtcgcg gcacgcaaga accgatcggt
     2221 ggttcgcaga tcgaccggtt cggcccccac gtatgcctgc ttccacgacg acagatccag
     2281 catctcgtcg gttctctcca gagcgcgtgc tgccagcgcg tatccgaacg tcggcgcggc
     2341 cgaagtctgg acccctcgat tcgcgatctc ctgcaaccat cgcagcggat ccgtaaggaa
     2401 cgtctcggtg agcatcatcg tcaaggtgga accacttcca agtgcaggca gcatgaagcc
     2461 catcaaaccg aggtcgtggt acagaggtac ccagccgagc ctggagccgt cccccgcgag
     2521 caagcgctgt tcttcagcca aggcgatctg agtcaatacg gcacggtgat tgagaacgac
     2581 acctttgggc tggctcgtgc ttccggaact gaattggatc agtgccacgt cctcgcctgc
     2641 aggcagcggc ccgtcataag cgcccgcatc ctcgagatcg actgcccata tcggcctcac
     2701 tcccagaggt tcgggatccc ctgctccgag caggacgcag gccgcatttg catccgagat
     2761 acgtcgcacc gcttgatcca tccatgcatc gggtgccagg aaccggggtg ggatcgggag
     2821 agggacccca acggctccca gacgccacac ggccagaagg tcggccatga agtcgaacga
     2881 cgtctgggcg aacagcgcca cgcggtcgcc ggggcggatc cccttctcgg caaggccccc
     2941 ggcccttagt tcggcgaact cgacgatctc ttggtggctt ctcgtctctt ccgtggaatc
     3001 accgacgatc gacagagagt taccaagccg agctccctca ttgaagactt ggggcagcgc
     3061 ggtagacgtc gtcgcatagc tcggcatact catttccttc tcgtttgtgg ctggcaggtt
     3121 actaaggagg gaacacctga gagtcctgtt cgtcagcccg gattacccac ccgatgtagt
     3181 gggcgggatc gggccgtaca ccgctgaagt ggcgccgcag cttgccgatc gtggtcacga
     3241 ggttcacgtg ttgtgttgca ggcagggtga acacaatgag gacgagaccc acggagcagt
     3301 gaccgttcat cgcagagggt ggcgcgacac tccaggccga ggcctcatca ctcgggcccc
     3361 cgaagccacc ctgcgctggc ggaatgctct cgaaactcga cgccacatcc gttctctcgg
     3421 ccacttcgac gcgatcgagg ttccagactg gaaggcattc ggtgcgttcc ttccgcgcgg
     3481 gatccctctc gtcttgcatc tgcacatgcc actccgagta cacgccgaat acgagccaga
     3541 gttcaccgac tgggatgtga cctgttccga tctcatcgag cgacgagcag tgccgaaagc
     3601 acttgtcgtg acttgcccat cgaacctcct tgctgatcgc gttcgaaatg gcccatggaa
     3661 gatcgggacg gacatccgga tcgttcggca tgcgatctcg cctctcccgt ggcagcaagc
     3721 gggccccgtc gcgaccacac cacctacggt cctcttcgtc ggccgcctgg agccgcgcaa
     3781 ggctcccgat ctactcatcg aaggtgctgc caaactcctc gagcgcgtcc ccgacttgaa
     3841 cgttgtattc gccggggcgg aaacgaacac agcgaaggga ctcgagttcg cgcgttcatt
     3901 gcgagtctct tcggagaggc tcggcgtgcc gtgcacgttc gccggcaagc aatcgcgaga
     3961 agagcttgtc ggatggcatg ctcgagcgcg tctagcggtg atccccagcc ggtttgacaa
     4021 cttcccgatg accggcttgg aggcgctcgc ggcggggcgg ccgctcgtag tcagtgatca
     4081 agtggggttg gccgagatca tcggcgacgc aggtgcagta tttccatccg gcgatccgga
     4141 cgcgcttgcc gcggcgatgc tcccttacct cctcgatccg gtcgcggccg gtcgagccgg
     4201 tgctcgagcg caggttctgg ctcgggacaa cttctctccg cagaagatcg cggccggacg
     4261 ggagagatgc ttcgccgagg ccgccgctct agtgagctca ccaacttagg acgggaggtt
     4321 cgacacctcc cccgttccgc cggagcactc gcttgacctt tcccgcgctg gcccacagcg
     4381 aagtgttgcc cagaacggat cgcgcgatcc cggtcgcatg gcgcagcgac caaggttgtt
     4441 ggagccacgc ggcggcaagg gccctccgag cggcccggta ttcgccgagc tcggattgga
     4501 taaacgcgag ctccgctaga tggtgcgcct tcagcgatct gcgctccaga ggatccagtc
     4561 gctcgatgcg cgggccgatg cgctcccaca tctgttgtcg ggcataaagc cttatggcgc
     4621 ggagatcccg atccgagtgc agttcacgac gcacaaggag ctcgggcacc aaggtcacgt
     4681 cgtaacgatc cgcgacgcgc agccagaggt cccagtcttc gtactgcgac aacgatgcgt
     4741 cgaacccacc ggcctcgatg aaagccgact tgcgtatcag caccgacgac ggagccatcg
     4801 aaggacgtcg caggagcgtt cgctgcagat cgcgatcggg atctggcacg aagctgaatc
     4861 gaagatctcc cgactcgcgc atctcccacc cgcactgcgc accggcgaac gagggaccgg
     4921 cctcaatcaa ggcgttcaca cgacgctcga cactcgccgg taaccactcg tcgtcgtcgt
     4981 ccaggaacgc gagtagctca ccgctggcgg cctcgacccc ggcgttcctt gctgctcccg
     5041 gcccacgcga tggttccagc cgcacgaggc gcgttggtgg atgatccgcc actacttccg
     5101 gctcgaccgg ttgagtcgat ccatcgtcga cgacgacgat ctcgagtgcg ggataggtct
     5161 gggacctgac gctggcgaga gccctgtgca acgatgccgg gcgatcccgg gtcgggacga
     5221 tggcagtcac gagaggaagc ctggtcatcg atccgtccta acctcgcctt gtgaagatcc
     5281 tctttgcggc ccacggtaat cccagtcgaa ccccaggcgg gatcgaaacg tacgcgaggt
     5341 gcatcgcccc cgcgctcgtg gatctcggcc acgaagtgca ccttctctgg ctcggcttcg
     5401 ggcagggggt ccacgatgga agggaaggcg gggtcacagt gcatcttcga ccgtggcggg
     5461 ggccgcgtgc cctaggccgg gcagtcggct tgcctcactc ctgggcgcgc atccgtgtag
     5521 ctcttgctgc caaagcagcc ctcacgcaaa ttggtcggcg tttcgatgtg atcgaagctc
     5581 ccgagtggat
//
LOCUS       DATMED010000235         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2121823, whole genome shotgun sequence.
ACCESSION   DATMED010000235 DATMED010000000
VERSION     DATMED010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121823"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="VNP73_06075"
     CDS             complement(<1..1673)
                     /locus_tag="VNP73_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWL65525.1"
                     /translation="MDLPIADYALIGDTRTAALCSSQGSIDWLCVPRFESDPVFGRLI
                     GGERAGRFFIAPEGVRSTSRSYRRDSAVLDTTWRTETGELILTEGMVLDVQKRLLPQA
                     LLVRRVRCTNGTATLRINFDPKLGLPGRSPRSERRNGDLVCTWGSLAVALRSQPDLAL
                     RPGQERIVELRAGEQLTLVMTMADRAPLVLISPDAAFAELARTDAWWQQWSSGLSYEG
                     EFRDPVARSLITLRLLTYSPSGAPVAAPTTSLPEVLGGSRNWDYRLSWPRDASIGIGA
                     FLGVGKTEEAHSFMHWLLHASRLTRPRLGVLYTIYGKPGPMETDLNEVAGYEGSRPVR
                     TGNAASSQHQLDVYGWVVDAAWLLSHSNKGLQSETRRAVAGLANFVSRTWREPDAGIW
                     EVRGEPRPYVHSKLMAWVTLDRAIRIDQNNGGRSRRHRHWVAERAALALDIQEHGYSA
                     KHNTYVWRYGSEQVDAALLILPIVEFENTTSARLAGTVAAIRRELDAGDGLLYRYAPG
                     ADDLDGAEGAFLPCPFWLVQALARVGQIEEAKGLFERLLGLANDVGLFS"
     gene            complement(1690..>2061)
                     /locus_tag="VNP73_06080"
     CDS             complement(1690..>2061)
                     /locus_tag="VNP73_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL65526.1"
                     /translation="LTSSFLCCRAALPWMRKSEWGRIVLVSARTARFDRTGQAAYAIA
                     KAGVGLLAEVISEENRSFGVTANAIAPSILDTPANRSAMPDADPSTWVSPEYVAATVE
                     FLCSEEAGDLRGAWLPLTGAA"
BASE COUNT          381 a          672 c          649 g          359 t
ORIGIN      
        1 tcgctgaaca gaccgacgtc gtttgccagc cccaacagtc gctcgaatag gccctttgcc
       61 tcctcgatct ggcccacgcg cgcgagagct tggaccagcc agaaagggca ggggaggaag
      121 gcaccttccg ctccatcgag atcgtccgcc cccggcgcgt aacgatagag caagccatcg
      181 ccggcatcta gctcacggcg gatggccgcc acagtcccgg caagacgcgc cgatgtggtg
      241 ttctcgaatt ccacgatggg caggatcaga agggccgcgt cgacttgctc cgacccgtac
      301 cgccacacat acgtgttgtg cttggcgctg tacccatgct cttggatatc gagggcgagc
      361 gcggctctct cggcgaccca gtgacgatga cggcgactcc gccccccgtt gttttggtcg
      421 attcggatgg cgcggtccag ggtgacccac gccatgagct tggagtgaac gtacggacgg
      481 ggctccccac gcacctccca gattccggcg tcaggttccc tccaggttcg cgacacgaag
      541 ttggccaagc ccgccaccgc gcgtcgcgtc tccgactgga gccctttgtt cgaatgggac
      601 aatagccacg cggcgtccac tacccaccca tagacatcga gctggtgctg ggagctcgca
      661 gcgtttccgg tcctcacggg ccggctgccc tcgtacccgg cgacctcgtt gagatctgtt
      721 tccatcggtc cgggttttcc gtagatcgtg tagagcaccc cgagacgagg acgcgtaagt
      781 cggctcgcgt gcaacagcca gtgcatgaac gagtgagcct cttcggtctt tcccacgccc
      841 aggaacgcac cgatcccgat gctcgcgtcg cgaggccacg acaaccggta gtcccaattc
      901 ctggagccac cgaggacttc gggcagcgac gtggtcggtg cagcgacggg cgcgccggaa
      961 ggagagtagg tcaatagccg cagcgtaatc aggctgcgcg cgacgggatc gcggaattcg
     1021 ccttcgtacg acagtccgct agaccattgt tgccaccacg catccgtgcg ggccagttcc
     1081 gcaaaggccg cgtcgggact aatgagtacg agcggtgctc gatccgccat cgtcatgacg
     1141 agagtcaact gctctcccgc gcgtaactcg acgattcttt cttgaccggg cctgagagcg
     1201 agatccggtt gggaccgaag cgcgaccgcc agggaacccc acgtacagac gaggtcgccg
     1261 tttcgtcgtt ccgagcgcgg gctgcggccc ggaaggccca gctttgggtc gaagttgatc
     1321 cgtagggttg ccgttccgtt cgtgcaccgg actcgccgta cgagcagagc ttgggggaga
     1381 agcctttttt ggacgtccaa gaccattcct tcagtgagga tgagctctcc cgtttcagtg
     1441 cgccaggtgg tgtcgagcac cgccgaatcg cggcggtagg agcgggatgt ggatcgcact
     1501 ccctccgggg cgatgaagaa gcgaccggca cgttcgcctc caatgagtcg gccgaacacc
     1561 ggatcggatt cgaaacgcgg gacgcacagc caatcgatgg atccctgcga agaacagagg
     1621 gcagcagtcc gcgtgtcccc gatgagtgcg tagtccgcaa tgggcaggtc catcagctcc
     1681 gcccggcagc tatgcggcgc cggtcaacgg cagccacgcc cctctcaggt cccctgcctc
     1741 ttctgagcac aggaactcga ccgtcgcggc gacatactcc ggagaaaccc aggtagaggg
     1801 gtccgcgtcg ggcatcgccg accgattggc cggcgtgtcg agaatggaag gagcgatcgc
     1861 gttcgccgtc acaccgaatg atcggttctc ctcggagatc acctcggcaa gcagtccgac
     1921 gccggccttg gcgatcgcgt aggccgcctg tcccgtgcgg tcgaagcgcg ccgttcgggc
     1981 cgacacgaga acgatcctcc cccattcgct cttacgcatc caaggcagcg cggctcggca
     2041 acaaaggaag cttgacgtga g
//
LOCUS       DATMED010000236         5639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3264627, whole genome shotgun sequence.
ACCESSION   DATMED010000236 DATMED010000000
VERSION     DATMED010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3264627"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VNP73_06085"
     CDS             complement(<1..356)
                     /locus_tag="VNP73_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWL65527.1"
                     /translation="MSTDANLLDRFAAAYGFKFDDFQLRACEALLDGSSVLVAAPTGA
                     GKTVVGEFAAWLALQRGGKTFYTTPIKALSNQKFGDFVGVYGPSDVGLLTGDNSVNGS
                     AQVVVMTTEVLRNMIY"
     gene            complement(353..1039)
                     /locus_tag="VNP73_06090"
     CDS             complement(353..1039)
                     /locus_tag="VNP73_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65528.1"
                     /translation="MAIKGKKRKPRQRSRRPGAPPRPSVASGRSTPWYQTTVGRIAAA
                     ATLLVILGVALFMINNARDDQRALERRQAAMEEFTGVIEVLLQKVGPPASEMVTAAAD
                     SKNLQQDAERWEGVLSEAQTDLTTQTAEAPFEVDVANRLIFQAIAQYRAAAATFALVP
                     EADEKLGKKILERAQADLAAADGTWSAAVAALDAEREDVDLRASGIRAPSTPATPPGG
                     GVPTTIPTGG"
     gene            complement(1059..1664)
                     /locus_tag="VNP73_06095"
     CDS             complement(1059..1664)
                     /locus_tag="VNP73_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65529.1"
                     /translation="MSDNELQSYDTFLVRQLIRPMVNVYTVTVGGDPKAPGPVVAFVK
                     QKRMAFKEQIRVFADEQASRELFNIKARRVIEIGGQYDVIGADGQAIGVLQKKFKQSL
                     VRSTWNVLDAMDSDVMLAQEKSAAIAVLRRVKEVFPYTDMIPLPYHFSFLRNEREIGS
                     LSRVMSFRDQYVLDLSGDVDGAVDRRLAIALAIALDALQGR"
     gene            complement(1661..2461)
                     /gene="tatC"
                     /locus_tag="VNP73_06100"
     CDS             complement(1661..2461)
                     /gene="tatC"
                     /locus_tag="VNP73_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWL65530.1"
                     /translation="MSDGAEVRRRRPSFTRRRTRAGSMTMMEHLRELRTRLIFSLAAF
                     LVVSVVAFFFYDPVLDLITRPLCDLPRDLLGPQGCRLIFVKVIGGFMFRLKVTALVGI
                     IFSTPVWLYQLWAFIVPGLTRKERRYAIPFVLTSVVLFALGTFLAYQSLPTGLRILFA
                     IAGEDLVAYTGAEEYLNFIGLMFLGFGLMFELPLVLFFLGLAEVISVDQLRRQRKAAL
                     VGIVALSAIITPSQDPYTMLILAGPIYLLYEVTILVLKIIMRRRAKAS"
     gene            complement(2454..2693)
                     /gene="tatB"
                     /locus_tag="VNP73_06105"
     CDS             complement(2454..2693)
                     /gene="tatB"
                     /locus_tag="VNP73_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HWL65531.1"
                     /translation="MPQIGPLEIVVVAMIALIVFGPEKLPGLARSIGRAMNELRRMAS
                     EVKTEFTADFDDEAVAPEPPAPEPTPRATPRETDE"
     gene            complement(2751..3083)
                     /locus_tag="VNP73_06110"
     CDS             complement(2751..3083)
                     /locus_tag="VNP73_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65532.1"
                     /translation="MTTIKANCPKCGEIDLTADDILLRIGSVKESNTYGFSCPDCNEF
                     VEKPADERIVRLLLSGGVMPMLVYVPAEALEAKEGPPIGYDDILAFHELLEGDDWFKE
                     LTKPSPHQ"
     gene            3222..5270
                     /locus_tag="VNP73_06115"
     CDS             3222..5270
                     /locus_tag="VNP73_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65533.1"
                     /translation="MALFARVRKELPQDPERWRAGLEAALTVSEIASTSSALPDAVQK
                     MVTAAIELLHAEQGSIMLLEDDGLTLVLAGSYGLPSEVPVGHSIRVGESVAGRVLATG
                     RPLLLGEVDSSVFINFVPKSRAITSSIVVPLRVQGRSIGVLSLAMSHGSPSFTEEDLR
                     LAQMFADQAAGLIFRTRLHEQAEHRSSDLMALVESSRGLVGSLDLEALLQHSLDGAAR
                     LAGTNEGFACLFDHDSGAIARGVFRSFAKDTIKAIVELPEIRQVIDSGDITFFERAGV
                     PLVALGLRTSQGTTGVLVVQADPEIAEDRRHLLRAFGQQCDTALGAAELHAEVQRKET
                     ELSAIIHGVPSPIVLIDARNKVIAVNSAAEQLFGISNAFVSGAALAGALGHDQIEKML
                     ASGGAFQAEVEAGSPPRTYKARMSNVRMPGVPMGRVLMLDDVTAEREIAQKQRDFVAM
                     IGHELRTPLTIIKGFARTFLRKGQKASPEDSAEALKTIDARAGLLERLIEDLLYVSQI
                     ETREASLRIDQVDIAVLVQGVTEDLISFQLEREAILEIPRPLVWPCDETKLALVLRHL
                     IENALKYSQAPHPVTIKAVDDGEELRIDIIDKGVGIVSSDIPDIFDRFRQVDASSTRE
                     HGGTGVGLYLCAQLVRMHNGRIWVDSTWGKGSTFSFTLPRRADTSDVVHIRGTEAATG
                     "
     gene            complement(5283..>5639)
                     /locus_tag="VNP73_06120"
     CDS             complement(5283..>5639)
                     /locus_tag="VNP73_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWL65534.1"
                     /translation="RMFRMDRMKSVAMLEEPSSVPDSFEPEEYRGAFRAEPDEPVIRF
                     EISPRAARWFSDYYPVVNEEAVEDGWHRIEMAVSGEHWAATLALQLGTEIRAIEPEQI
                     NERAADLARVLTESHS"
BASE COUNT         1049 a         1814 c         1742 g         1034 t
ORIGIN      
        1 tcgtagatca tgtttcgcag gacctcggtc gtcatgacaa ctacctgggc gctgccgttg
       61 accgagttgt ctccggtcag caagccgacg tcgctcgggc cgtacacgcc tacgaagtcc
      121 ccgaacttct gattggagag agctttgatc ggcgtcgtat agaaggtctt gcccccgcgc
      181 tgaagcgcga gccacgcggc gaactcccca acgacggtct tgccggcccc cgtcggggcg
      241 gccacgagga ccgaagaacc gtcgagcagc gcctcgcacg ctcgcagctg gaagtcgtcg
      301 aatttgaagc cgtatgcggc cgcgaagcga tctagaaggt ttgcgtccgt actcagccgc
      361 ccgtagggat cgtggtgggc acgccgccgc ccggcggcgt cgcgggcgtg gagggtgccc
      421 tgatcccaga cgctcgcagg tcgacgtcct ctctctccgc gtccagtgct gcaaccgcag
      481 ccgaccaggt accgtccgcc gctgccagat cggcctgggc acgctcgagg atcttcttgc
      541 cgagtttctc gtctgcctcg ggcacgagag caaacgttgc tgccgcggcc cggtactggg
      601 cgatcgcttg gaagatgagt ctgttcgcga cgtcgacctc gaagggggcc tcggcggttt
      661 gggtggtgag gtcagtctgg gcttctgaca ggaccccttc ccaccgctcg gcatcctgct
      721 ggagattctt cgaatcggcc gcggcggtca ccatctcgga ggccggagga ccgaccttct
      781 ggaggagtac ctcgattaca cccgtgaact cctccatcgc agcctgcctg cgttccaagg
      841 cccgttggtc gtcgcgcgcg ttgttgatca tgaagagcgc gaccccgagg atcacgagca
      901 gcgtcgcggc tgcggcgatc cgaccgacgg tcgtttggta ccagggggta ctcctaccgg
      961 aggcgacact ggggcgaggt ggtgccccgg gcctgcgcga acgctgacgc ggcttgcgct
     1021 tcttgccctt gatcgccatg gcagcgattt tagagcgttt atcgcccttg cagcgcgtcc
     1081 agagctatcg ccagtgcaat cgccagtctg cgatcgaccg ccccgtctac gtccccagaa
     1141 aggtccagga cgtattggtc tctgaaactc atcacgcgac tcaaactgcc gatctccctc
     1201 tcgttccgaa ggaatgagaa gtgataaggc agcgggatca tgtcggtgta ggggaagacc
     1261 tctttgacgc gtctcaatac cgcgatcgct gcgctcttct cctgggccag catgacgtca
     1321 gagtccatcg catcgagcac attccaagtg gatcgcacca gcgactgctt gaacttcttt
     1381 tggagcacgc ctatcgcttg cccgtcggcc ccgatcacgt cgtactgtcc gccgatctcg
     1441 atcacacgac gagccttgat gttgaacagc tcgcgcgacg cctgctcgtc cgcgaacacg
     1501 cggatctgtt ccttgaacgc catccgcttc tgcttgacga acgcgacgac cgggccgggc
     1561 gccttggggt ctcccccgac ggttaccgtg tagacgttca ccatcgggcg gatcagctgc
     1621 cgcaccagga atgtgtcgta gctctgcagt tcgttgtcgc tcatgatgct ttcgcccgcc
     1681 ttcgcatgat gatcttcaac accaagatag tcacctcgta cagtagatag atcggcccgg
     1741 caaggatcag catcgtgtag ggatcctggc tcggcgtgat gattgccgac agcgccacta
     1801 tccctactag agcagccttc cgctgcctcc tcaactggtc tacagatatc acttcggcaa
     1861 gtccgaggaa gaacagaacc aaaggaagct cgaacatcag gccgaagccg aggaacatca
     1921 ggccgatgaa gttcaggtac tcctccgccc ccgtgtacgc caccaggtcc tccccggcga
     1981 tcgcgaacaa gatcctgagt ccggtgggca acgactggta agcgaggaac gtcccgagcg
     2041 cgaacagaac gaccgaggta agcacgaacg ggatcgcgta ccggcgctcc ttcctcgtca
     2101 gcccgggcac gatgaaggcc cacagctgat agagccacac gggcgtcgag aagatgatcc
     2161 cgaccaaggc cgtcaccttc aggcggaaca tgaacccccc gatcaccttc acgaagatga
     2221 gccggcaccc ctgggggccg agaagatcgc ggggaaggtc gcagagcggt cgggtgatga
     2281 gatcgaggac ggggtcgtag aagaagaagg caacgaccga gaccaccagg aaggccgcca
     2341 gcgagaagat cagtcgggtg cggagctccc ggaggtgctc catcatcgtc atggagccgg
     2401 ctcgggtcct cctgcgcgtg aaggacggcc gcctgcggcg cacctcggcg ccgtcactca
     2461 tctgtttctc gaggggttgc tcggggggtc ggctcgggcg caggtggctc cggagccacc
     2521 gcctcgtcgt cgaagtcggc cgtgaactcg gtcttgacct ccgaggccat tcggcggagc
     2581 tcgttcatcg ccctgccgat cgaccgggcg aggcccggca gcttctcggg gccgaacacg
     2641 atgagagcaa tcatcgccac gacgacgatc tcgaggggac ctatctgagg catgacgcgc
     2701 gagtctaggg gcggttgccc ccggactcgg ttaaaggttt ggttggagag ttactggtgc
     2761 ggcgacggct tcgtcagttc cttgaaccag tcgtcacctt cgagcaactc gtggaaagcg
     2821 aggatgtcgt cgtaaccgat cggagggccc tccttcgcct ccagggcctc ggccggcaca
     2881 tacaccagca tcggcatcac gccacccgac aggagcaacc gaacgatccg ctcgtcggcg
     2941 ggcttctcca cgaactcgtt gcagtccggg cacgagaaac cgtaggtgtt ggattccttg
     3001 accgagccga tccgaagcag gatgtcgtct gctgtcagat cgatctcccc gcacttgggg
     3061 cagttcgcct tgatcgtcgt catctcaagc ctctttctcc cagctcccag ttccttcgtt
     3121 tctagttatc ggcgtctctc tcgccgggtt tagaccccct ccatacgtat ttctgcgggc
     3181 ttaacccctg gggtcggcaa gccgacaatc tctacagaga gatggctcta ttcgcgcgcg
     3241 tgcgcaagga actgccacag gatccagagc ggtggagagc ggggctcgaa gcagcgctga
     3301 ccgtcagcga gatcgcttca acctcatccg ccttacccga cgccgtccag aaaatggtaa
     3361 cggcggccat cgagctcctc cacgcagagc aaggctcgat catgctcctc gaggacgacg
     3421 gactcacgtt ggtgctggca ggctcctacg ggctgcccag cgaggtcccg gtcggccatt
     3481 cgatcagggt cggcgaaagc gtcgccggtc gggtgctcgc caccgggagg ccgttgctcc
     3541 tgggtgaggt cgattcctcg gtcttcatca acttcgtccc caagagtcgg gcgatcacct
     3601 ccagcatcgt ggtccccctg agggtccaag gtcgctcgat cggcgtgttg agcctcgcca
     3661 tgtcacacgg ttcccccagc ttcaccgagg aagacctccg gctggcgcag atgttcgcgg
     3721 atcaggccgc gggattgatc ttcagaacga gactgcacga gcaagccgaa caccggtcct
     3781 ccgacctgat ggctctcgtc gaatcgagcc ggggactcgt cgggtccctg gacctggagg
     3841 cattgctgca gcactccctc gacggagcgg cgcggctcgc cggaacgaac gagggtttcg
     3901 cgtgcctgtt cgaccacgat tcgggagcga tcgcgcgcgg cgtcttccgg agcttcgcca
     3961 aagacacgat caaagccatc gtcgagctgc ctgagatccg gcaggtcatc gattccggcg
     4021 acatcacgtt cttcgagcgc gccggtgtgc ctctggtcgc actgggatta aggacgtccc
     4081 agggcacgac gggagtcttg gtcgtgcagg ccgatcccga gatcgccgag gaccgccggc
     4141 acctgctccg cgccttcggc cagcagtgcg acacggccct cggtgccgca gagcttcacg
     4201 ccgaggttca aaggaaagag acggaactgt ccgcgatcat ccacggcgtg cccagtccga
     4261 tcgtcctgat cgacgcgcgg aacaaagtga tcgcagtcaa ctcggcagcc gaacaacttt
     4321 tcggcatctc gaacgcgttc gtctcggggg cggcgctggc cggcgcgctg ggtcacgatc
     4381 agatcgagaa gatgctggca agcggtggtg cattccaggc cgaggtcgaa gccggttcac
     4441 cccctcgcac ctacaaagcg cgcatgagca acgttcgcat gccgggtgtc cccatgggcc
     4501 gcgtgttgat gctcgacgac gtgacggccg agcgtgagat cgcccagaag cagcgcgatt
     4561 tcgtcgcgat gatcggacac gagttgcgca cacccctaac catcatcaag ggattcgctc
     4621 gtacgttcct gcgtaagggt cagaaagcat cgcccgaaga cagcgcggag gccctcaaga
     4681 cgatcgatgc ccgagccgga cttctggagc gtctaatcga ggatctcctc tacgtgtccc
     4741 agatcgagac gcgagaagcc agcctgcgga tagaccaggt cgatatcgcg gtcttggtcc
     4801 agggagtcac cgaggacctg atcagcttcc agctggagcg agaagcgatc ctcgagatcc
     4861 ctcgaccgct ggtgtggccg tgcgacgaga cgaagctggc gctcgtactc aggcacctga
     4921 tcgagaatgc cctcaagtat tcgcaggcac ctcaccccgt cacgatcaaa gcggtggacg
     4981 acggagaaga gttgcggatc gacatcatcg acaagggtgt ggggatcgtc tcgagcgaca
     5041 tccccgacat cttcgatagg ttccgccaag tcgacgcgtc ctccacgcgc gaacatggag
     5101 gcaccggggt cgggctttac ctgtgcgcac agctcgtgcg gatgcacaac gggcgcatct
     5161 gggtcgactc gacgtggggg aagggctcga cgttctcctt cacgctaccg agacgagccg
     5221 acacgagcga cgtggtgcac atccgcggca ccgaagccgc taccggctga ccggccgaac
     5281 tctcaggagt gagactcggt tagcacccgt gccagatcgg ccgcgcgttc gttgatctgc
     5341 tccggttcga tcgcccttat ctctgtcccc agctggaggg caagggtggc agcccagtgc
     5401 tcccccgaca ccgccatctc tatccggtgc cagccgtcct cgacggcttc ttcattcacc
     5461 accgggtaat agtccgagaa ccagcgcgcc gcgcgtgggg agatctcgaa acggatcacc
     5521 ggttcgtcgg gctcggcccg gaacgcgccc cggtactctt cgggctcgaa cgagtcggga
     5581 acgctggatg gctcttccag catcgccaca ctcttcatcc ggtccatgcg gaacatccg
//
LOCUS       DATMED010000237        11411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5552036, whole genome shotgun sequence.
ACCESSION   DATMED010000237 DATMED010000000
VERSION     DATMED010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5552036"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VNP73_06125"
     CDS             <1..1262
                     /locus_tag="VNP73_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HWL65535.1"
                     /translation="LGFDVVYLPPIHPIGAAHRKGKNNTLSAGPDDIGVPWAIGSKDG
                     GHKDIHPDLGTIEDFDSFVTEAGRLGLEVALDFAIQCSPDHPWVKEHPEWFTTRPDGS
                     IQYAENPPKKYQDIYPIDFETNDRVGLWTELKSVLDHWISHGVKIFRVDNPHTKSFPF
                     WEWVIGEITATDPGVIFLSEAFTRPKVMQHLAKLGFTQSYTYFTWRNEKWEIEEYLTE
                     LTQSEMAMYFRPNFFANTPDILHEYLQVGGPPAFKIRLILAALLSPSYGLYSGFELFE
                     NIPLREGSEEYLNSEKYELKHRDYNSGHSLVPYITRLNELRHKHPALAELTNLRFHNI
                     DKDHLMCFSKTVAGYDPIVVVVNLNPFHWEEGTVNLDLGVLGIEEGQAFEVNDLLTDT
                     TFVWHGSANYVRLDPFEEPAHIFRVRA"
     gene            1273..2961
                     /gene="treS"
                     /locus_tag="VNP73_06130"
     CDS             1273..2961
                     /gene="treS"
                     /locus_tag="VNP73_06130"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWL65536.1"
                     /translation="MTPLEPNPLWYKTAVFYEAYVRSFYDSNSDGFGDFKGLTEKMDY
                     LEWLGIDCLWLLPFYQSPLRDGGYDISDFYSILPEYGNLNDFMEFLDAAHSRGIRVVA
                     DLVMNHTSDQHPWFQEARVPGSEKRDWYVWSETPDRYPDARIIFLDTEKSNWAWDESA
                     EAYYWHRFFTHQPDLNYDNPEVQEQMLAVLRFWLDLGLDGFRLDAVPYLFEREGTNCE
                     NLVETHEFLKRVRSEVDKSYDDVVLLAEANQWPPDVVPYFGEGDECHMAFHFPLMPRM
                     FMAARRQVRYPVVEILEQTPPIPDNAQWGIFLRNHDELTLEMVTDEERDYMYGEYAKD
                     PRMKLNLGIRRRLAPLLENNRDQIELFHAMLLSLPGSPVIYYGDELRMGDNIYLGDRD
                     GVRTPMQWTADRNGGFSQCDFAQLYLPTLMDPVYGFQAVNVEAQLRNQHSFLQWFRRL
                     LAVRKEHTLFGTGTMDVLHPENAAIFAYVRRRGDDTILVVNNLSPRAQAAELDLSDYE
                     GLYPIELLGREKFPRIGELPYLLTFGPFSFYWFQLVEEEAEDGGGGNDGGGGNE"
     gene            2958..4379
                     /locus_tag="VNP73_06135"
     CDS             2958..4379
                     /locus_tag="VNP73_06135"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65537.1"
                     /translation="MKLDVSPLLETLPTARWFGGKGRPLKQVEIVDTGVIEDGPPALV
                     LTIVRVSFGDEGPDQLYHLPLIVEDGAARDALEDLDRLGVLGDLMAHGTSIKGADGVF
                     QFGGPGLDPLSPPGAESTRALGSEQSNSSLVLDDQIVLKLFRKVEVGPNPDLELNRHL
                     TSEGFQHVPLHVAEIIYEGEMGGEEISIDLAIAQQLVPNSVEGWTLVTQHLREFYDEV
                     DERDAREDMRFLTEERAKETLLHIDRLGEVTALLHIALTRFEGDVDLAPEPIERFDLE
                     AWAERARRSLVDLVKGPAPELKASAGQINDRINQLRTVEDAGMKLRIHGDYHLGQAIN
                     STRGWMILDLEGEPARSLEERRQKQSALRDVAGLLRSLNYAAMSVMFERSQPGEDAWN
                     RLAPWAEIWESVARERFLAAYLSRSHEGRFLPSDRETLMTMLDVFEIDKALYELGYEH
                     SHRPDWILIPLQGISQVLEREGR"
     gene            4376..6544
                     /gene="glgB"
                     /locus_tag="VNP73_06140"
     CDS             4376..6544
                     /gene="glgB"
                     /locus_tag="VNP73_06140"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWL65538.1"
                     /translation="MNKALKDQIGRLTRGELRDPHSLLGRHPLNGTVVVRAWRPEADS
                     VRVLQDGKAVAELDLIEPAGVFEGHIEGDLKDYELEVAYPNGDRFVQRDPYAFLPTLG
                     DLDLHLVGEGMHYRLWDKLGAHPTTIDGVDGVSFAVWAPNARSVRVVGEFNSWDGRLH
                     PMRSLGSSGIWELFIPDLGPGTLYKYEVMTAQGNLVLKTDPYAQATEAPPATASRVYE
                     SRYEWSDGSWIKRRADTNAYESPMSVYELHLGSWRRGSDGEVLGYRELGTELAEYCKE
                     LAFTHVELMPVAEHPFTGSWGYQVSNYFAPTARYGTPDDFRFFVNTLHQAGIGVIVDW
                     VPAHFPKDEFALARFDGTALYEHVDPRKGEHAEWGTLVFNYGRNEVRNFLISNALYWI
                     EEFHIDGLRVDAVASMLYLDYSREEGEWVPNEYGGNENLEAVAFTKQLNTVVHGEHPG
                     VLMMAEESTAWPGVSRPIHLGGLGFGFKWNMGWMHDTLSYFQKEPIYRRFHHHQMTFS
                     MMYAFSENFILPLSHDEVVHGKGSLINKMAGDKWQRMANLRCLLAFMWAHPGKQLLFM
                     GGEFGMEREWDHDESIDWHLLDDPQHRGVQQLVGDLNRAYRDTRALWQQDFTPDGFRW
                     IDANDADNNVLSFCRVSADGNEHLVCIANLAPIPRSGFRVGLLKGGDFTEVLNTDSEA
                     YGGTNTGNMGIVKSRPVAWHGLDHSAMLTLPPLGVVWLRG"
     gene            6541..8055
                     /locus_tag="VNP73_06145"
     CDS             6541..8055
                     /locus_tag="VNP73_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HWL65539.1"
                     /translation="MSGTSRICVHGHFYQPPRENPWTGEIDMQPSAAPYHDWNERVHA
                     ECYRPNAFARIPAGPPAEGAEEDEFEVVNNFERLSFNVGPTLLAWMERADPEAYRKIV
                     EADAASVDRLGHGNAIAQAYHHTILPLSPSHEIRTQVRWGIADFKNRFGRDPAGMWLP
                     ETAANDDVLAILIEEGIGFTILAPKQASRWREEGGRWIQAEEEPVDPSIPYRYLHRDG
                     SGRSLTLFFYDGEIARKIAFERAGSSAEGFVRLFEERAGSGRLVHAATDGETYGHHHV
                     FSDLGLAYALFVEAGERDLEVTNYAAYLADAEVTHEATIWRGEGTAWSCAHGVGRWVR
                     DCGCSTGGDEGWNQRWRGPLRAGLDIVKHTADEVYERLGGALLSDPWPARDDYVNVVL
                     GAQGLDEFVAHHSSGPSDETVARQIETLLEMQRNAMSMYTSCGWFFNDISGIETIQVL
                     RYAARTLDLIEELGQPTPEASFLATLEQARSNDRSQGTGADIYLALVSAPMAAR"
     gene            8082..9596
                     /locus_tag="VNP73_06150"
     CDS             8082..9596
                     /locus_tag="VNP73_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65540.1"
                     /translation="MKRTARSARRSRKLMVVVLCSALAFAAGSTPLPHATGQAGPGYF
                     ASENVEWIKTLPQETDTSGGVFFDKHLYVTTARALTIYDISDPENPAITGTHPLPQQP
                     QFSEEDPDTNGKILLIGTLGILFVFDVEDKTNPQIVGQLDGADEHTISCVLDCKWGYG
                     SEGAIVDLRDPTNPKMAGDWGEGNPATSGHDVTEVAPGLIVTSSNPMMLLDARKDPTK
                     PKVVALGKPPDNRLIHGNLWPGGGTDRFLLVGGETTGNCGSETSGAFMVWDAAKVGVT
                     RTFTMIDELRMVNGNPAEGNAPANSFCAHWFTTHPTYKDGGLVAMAWYEHGTRFLQVT
                     SEGKIKEVGWFLPIGGATSASYWVTDEIVYALDYQRGLDILRYTGKFYEPTGGGGGTG
                     GGGGGGSGSGEQLPGPGVRMRISDRTPARDEVVRFRVLLRKCPRSHYRTLVELQRKRR
                     DRFFTVTEAKLNRNCRAVMRVPAGFDKATFRAIWPKQDDDHRAGRSKPQTVTPH"
     gene            complement(9632..>11411)
                     /locus_tag="VNP73_06155"
     CDS             complement(9632..>11411)
                     /locus_tag="VNP73_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWL65541.1"
                     /translation="TPNSVAHGDFRESIVMSTGRGVYAWAPTVLNWVDLNAPRAGIRG
                     LLADRTSNPSFYGHTTRTVEIYSGPTGEDPEFDIPEDIIEIPDISLLDVAPPDVARQT
                     TLTPKQTTVRLDIGKRRTVTYDVGVPRTLTPLDVFFVVDTSSSMTQVIKEAAEALETI
                     YRGLASSGAAVQFGLAEYRSYPIDIPPKSETDNYVYRRVLDVGSTTAQMEDAIEGLTA
                     AGGGVYDAQLEALWQAATGNGADVWPPGPSSRDVPPGIQANFRKNALRVVLHVGDEPF
                     GREDSASDSDNTVTRPESELAKPDIPEFETVAAALRANGVKQLGLSLYPDATPDLRRM
                     ADATGAFAPPGGVDCDRDGSTDVPAGEPLVCVLQQDEIDDANMAPAIVELVEAVRTKS
                     SVGLKVDPERDGVLQSVSPDRYNQVLLQADSSLEFDVTYRCPESLAGKEIDVDLQATG
                     LIGTPPVSQTRVVCAEIPPDKPPLGIPFDRFVALVPLIPIGPPPPPVNVSSATQAQAQ
                     AQANAAFAAQEQEQPQVAMVHQLHADAQSALAREEQYSFTSLRKDPGPPVELWGAALL
                     MSAGFGVAMNTQRRTRLRFAKVRSRR"
BASE COUNT         2201 a         3593 c         3611 g         2006 t
ORIGIN      
        1 agctcggctt cgacgtcgtc taccttccgc cgatccaccc gatcggggct gcgcatcgca
       61 agggtaagaa caacaccctc tccgccggtc ccgacgacat cggagtgcca tgggcgatcg
      121 gaagcaagga cggcggacac aaagacatcc accccgatct cgggacgatc gaggacttcg
      181 attcgttcgt gaccgaagcc ggccggctcg ggctggaggt cgcgctcgac ttcgcgatcc
      241 aatgctcacc cgaccatccc tgggtcaaag aacatcccga gtggttcacc acgcgacccg
      301 atgggtcgat ccaatacgcg gagaatcccc cgaagaagta ccaggacatc tatccgatcg
      361 acttcgagac caacgacagg gtcggcctat ggaccgagct caagagcgtc ctcgaccact
      421 ggatctcgca cggagtcaag atcttccgtg tcgacaaccc gcatacgaag tcgttcccct
      481 tctgggaatg ggtgatcggt gagatcaccg ccacggatcc cggtgtgatc ttcctatcgg
      541 aggccttcac ccgaccaaag gtgatgcaac atcttgcgaa gctgggattc acccagtcgt
      601 acacctactt cacgtggcga aacgagaagt gggagatcga ggaatacctg accgaactaa
      661 cgcagtccga gatggcgatg tacttccgtc ccaacttctt cgccaacaca cccgacatcc
      721 ttcacgagta cctacaggta ggaggaccac ccgcgttcaa gatcagactg atactcgcgg
      781 cgcttctctc ccccagctac ggtctctatt cggggttcga gttgttcgag aacatccccc
      841 tgcgcgaagg cagcgaggag tacctgaact cggagaagta cgagttgaag catcgcgact
      901 acaacagcgg ccacagcttg gtcccttaca tcacgcggtt gaacgagctc cggcacaagc
      961 acccggcact ggccgagctc acgaacctcc gcttccacaa catcgacaag gaccacctca
     1021 tgtgcttctc gaagaccgtc gccggatacg acccgatcgt cgtcgtggtg aatctcaatc
     1081 ccttccactg ggaagagggg acggtcaacc tggatctcgg cgtactgggc attgaggagg
     1141 gacaggcctt cgaggtgaac gacctgctca ccgacacgac gttcgtgtgg cacggctcgg
     1201 cgaactacgt acgtctcgac cccttcgagg aaccggcgca catcttcagg gtccgggcct
     1261 aagctcggac ttgtgacacc actcgagccg aatccgctct ggtacaagac ggccgtcttc
     1321 tacgaggcct acgtccgctc cttctacgac tcgaactcgg acggattcgg ggacttcaag
     1381 ggcctgacgg agaagatgga ctacctggag tggttgggca tcgattgctt atggctgctc
     1441 cccttctatc aatccccgtt gcgcgacggt ggctacgaca tctccgactt ctattcgatc
     1501 ctgcccgagt acgggaacct gaacgacttc atggagttcc tcgacgcggc ccacagccgc
     1561 ggcatccgcg tcgtcgccga cctggtgatg aaccacacgt ccgaccagca tccgtggttc
     1621 caagaggcgc gggtgccggg atcggagaag cgggactggt acgtgtggtc ggagacgccg
     1681 gatagatacc cggacgcgcg catcatcttc ttggacacgg agaagtcgaa ctgggcgtgg
     1741 gacgagagtg ccgaggcgta ctactggcat cgcttcttca cccaccagcc cgacctcaac
     1801 tacgacaacc ccgaggtgca agaacagatg ctcgcggtgc tgaggttctg gctcgacctg
     1861 ggtctcgacg gattccgtct cgatgcagtg ccttatctgt tcgagcgaga gggcaccaac
     1921 tgcgagaacc tggtggagac gcacgagttc ctgaagcggg tgcggtccga ggtcgacaag
     1981 agttacgacg acgtggtgct gctggccgag gctaaccaat ggccgcccga cgtcgttccg
     2041 tacttcgggg agggcgacga gtgccacatg gcattccact tccctctcat gccgcggatg
     2101 ttcatggcgg cccgcagaca ggttcgctat ccggttgtcg agatcctcga gcagaccccc
     2161 ccgatccccg acaacgctca atggggcatc ttccttcgca accacgacga gctgacgctc
     2221 gagatggtca ccgacgaaga gcgggactac atgtacggcg aatacgcgaa ggaccctcgc
     2281 atgaagctca acctcgggat ccgccggcgc ctggctcccc tactcgagaa caaccgcgac
     2341 cagatcgagc tcttccacgc gatgttgctg tcgcttcccg gttccccggt catctattac
     2401 ggcgacgagc tgcgcatggg cgacaacatc tatcttggtg atcgcgatgg tgtccgcaca
     2461 ccgatgcaat ggaccgcgga ccgtaacggt ggattctcgc agtgtgactt cgcccagctg
     2521 tacctgccga cattgatgga tccggtgtac ggcttccagg cggtcaacgt cgaggcccag
     2581 ctccgcaacc agcactcgtt cctgcagtgg ttccggcggc tccttgcggt gcgcaaggag
     2641 cacaccctgt tcggaaccgg caccatggac gtgctccatc cggagaacgc ggcgatcttc
     2701 gcttacgttc gtagacgagg cgacgacacc atcctggtgg tgaacaacct gtcgccgcgg
     2761 gcacaggcag ccgagctcga tctgtccgac tacgaggggc tatatccgat cgagctactg
     2821 ggtagggaga agtttccgag gatcggcgaa ctcccctacc tgctgacctt cgggccattc
     2881 agcttctatt ggttccaact cgtggaggag gaggcagagg atggtggagg agggaacgac
     2941 ggtggaggag ggaacgaatg aagcttgacg tgagcccact cctcgaaacg cttcccactg
     3001 cacgctggtt cggcggcaag gggaggcccc tcaagcaggt cgagatcgtc gacacgggcg
     3061 tcatcgagga cggcccgcct gcgctggtac tgacgatcgt gcgggtgtcg ttcggagacg
     3121 aagggccgga tcagctctac cacctgccac tcatagtgga ggacggtgcc gcgcgcgatg
     3181 ccctcgaaga cctcgaccgt ctcggcgtcc tcggcgacct catggcccac ggcacctcga
     3241 tcaagggggc cgacggggtg ttccagttcg gggggccggg tctcgacccg ctgtcgcctc
     3301 cgggggccga gagcacccga gcgttggggt ccgagcagag caactcctca ctcgtgctcg
     3361 acgaccagat cgttctgaag ctcttccgta aggtcgaggt aggtcccaac cccgacctcg
     3421 agctgaaccg ccatctaacg agcgaaggtt tccagcacgt tccgcttcac gtcgccgaga
     3481 tcatctacga gggcgagatg ggaggcgagg agatatcgat cgacctcgcc atagcgcagc
     3541 agctcgtgcc caactccgtc gagggatgga cgctggtcac ccagcatctg cgcgagttct
     3601 acgacgaggt agacgagcgc gatgcgcgcg aagacatgcg cttccttacc gaggagcggg
     3661 ccaaggaaac gcttctccat atcgaccgac tcggagaggt cacggccctg ctccacatcg
     3721 ctctgaccag attcgagggt gatgtggatc tcgctccgga accgatcgag cgtttcgatc
     3781 tcgaagcctg ggccgaaagg gcgcgccggt cgctcgtgga cctggttaag ggaccggctc
     3841 ccgaactcaa agcatcggcc gggcagatca acgaccgcat caaccagttg cgcactgtgg
     3901 aagacgcggg catgaagctg cgcatccacg gcgactacca cctgggtcag gcgatcaact
     3961 cgacgcgcgg atggatgatc ctcgacctcg aaggcgagcc ggctcgatct ctcgaagaac
     4021 gccggcagaa gcagtcggcc ctaagagacg tcgccggatt gctccgatct ctgaactacg
     4081 ccgcgatgag cgtcatgttc gagcgatctc aacccggcga ggatgcatgg aaccggctcg
     4141 caccctgggc cgagatctgg gagtcggtgg cgcgggaaag gttcctcgcc gcatacctgt
     4201 cgcgttcgca cgaaggaaga ttcttgccga gcgaccgaga gacgttgatg acgatgctcg
     4261 acgtgttcga gatcgacaag gccctctacg agctcggtta cgagcacagc catcgccccg
     4321 actggatcct catccctttg cagggaatct cacaggtgtt ggagcgagaa ggacgatgaa
     4381 caaggctctg aaagatcaga tcggccggct gacccgcggc gagttacgtg acccacattc
     4441 gttgctcggc cgccaccccc ttaacggcac ggtcgtcgtg cgcgcgtggc ggcccgaagc
     4501 cgattccgtg cgggtgcttc aagacggcaa ggccgtcgcc gagcttgact tgatcgagcc
     4561 cgccggagtc ttcgaagggc atatcgaagg cgatctcaag gactacgagc tcgaggtcgc
     4621 gtatccgaac ggagatcgct tcgtgcaacg cgacccctac gccttcctcc ccaccctcgg
     4681 cgacctggat ctgcatctcg tcggcgaagg gatgcactac aggctgtggg acaagctcgg
     4741 cgcccacccg accacgatcg atggtgtcga cggcgtttcg ttcgcggtgt gggcaccgaa
     4801 tgctcgcagc gtccgggtgg tcggcgagtt caactcgtgg gacggcaggc tccacccgat
     4861 gcggtccttg ggctcatcgg gtatctggga gctcttcatc cccgacctcg gaccgggcac
     4921 gctctataag tacgaagtga tgacggctca gggcaacctc gtgctgaaga ccgaccccta
     4981 cgcacaagcg accgaggcgc caccggctac cgcctctcgc gtctacgagt ctcgttacga
     5041 atggagcgat gggagctgga tcaaacgccg tgccgacaca aacgcgtatg agtccccgat
     5101 gtcggtctac gagctgcatc tcggatcgtg gcgcagaggg agcgacggcg aggtgctggg
     5161 ttaccgcgag ctgggtaccg aactggccga gtactgcaag gagttggcgt tcacgcacgt
     5221 cgagctgatg cccgttgcgg agcacccctt caccggatcg tggggctacc aggtcagcaa
     5281 ctacttcgcg cctactgccc gctacgggac tccagacgac ttccggttct tcgtcaacac
     5341 cctgcatcag gcgggtatcg gcgtgatcgt cgattgggtg ccggcgcact tccccaagga
     5401 cgagttcgca ctggcccggt tcgacggcac tgctctgtac gagcacgtcg atccacgcaa
     5461 aggggaacat gccgagtggg gaactctagt gttcaactac ggccgcaacg aggtgcggaa
     5521 cttcctgatc tcgaacgcgc tgtactggat cgaggagttc cacatcgacg ggctgcgcgt
     5581 tgatgccgtg gcctcgatgc tgtacctgga ttactcgcgg gaagaaggcg agtgggtccc
     5641 gaatgaatac gggggcaacg agaacctcga agcggtcgcg ttcacgaagc agctcaatac
     5701 cgtcgtgcac ggtgagcatc ccggtgtgct gatgatggcc gaggagtcga ctgcgtggcc
     5761 gggcgtcagc cggcccatcc atctcggcgg cctcggattc ggcttcaaat ggaacatggg
     5821 ctggatgcac gacaccttgt cgtacttcca gaaggagccg atctatcgcc gcttccacca
     5881 ccaccagatg acgttctcga tgatgtacgc gttctcggaa aacttcatac tgccgctgtc
     5941 gcacgacgag gtcgtgcacg gcaaaggctc tctgatcaac aagatggccg gtgacaaatg
     6001 gcagcgcatg gcgaacctgc gctgtctcct tgctttcatg tgggcgcacc ccggcaagca
     6061 gctgctgttc atgggcggcg agttcggtat ggaacgggag tgggatcacg acgagagcat
     6121 cgactggcac ctgctcgacg atcctcaaca ccgtggggtc cagcagctcg tcggggacct
     6181 caaccgggcc tatcgcgaca cgagggcgct gtggcaacaa gacttcacac ccgatgggtt
     6241 ccgatggatc gacgccaacg acgcggacaa caacgtcttg tccttctgca gggtgagcgc
     6301 ggacggcaac gagcatctcg tctgcatcgc gaacttggct cccatcccgc ggagtggatt
     6361 ccgggtgggc cttctgaagg gtggagattt caccgaggtc ctgaacaccg actccgaggc
     6421 ctacggaggg acgaacacgg gcaacatggg gatcgtcaag tcgcgaccgg tggcgtggca
     6481 tggactggac cactccgcga tgctgacact ccccccgctc ggagttgtgt ggttgcgagg
     6541 ttgagcggca cgtctcggat ctgcgttcac gggcacttct accaaccacc gcgggagaac
     6601 ccgtggacgg gcgagatcga catgcaaccg agcgccgcgc cgtatcacga ctggaacgag
     6661 cgcgttcatg ccgagtgtta ccggccgaac gcattcgcgc gtatcccggc gggacccccc
     6721 gccgagggag ccgaagaaga cgagttcgag gtcgtgaaca acttcgagcg cctgtctttc
     6781 aacgtcggac ccacgcttct ggcatggatg gagagggccg accccgaggc ttaccgcaag
     6841 atcgtcgaag cggacgctgc gagcgtggac aggctggggc acgggaacgc gatcgcgcag
     6901 gcgtaccacc acacgatcct tcctctatcc ccttcgcacg agatccggac tcaggtgcgc
     6961 tggggtatcg cagatttcaa gaatcgcttc ggacgcgatc cggccgggat gtggctaccg
     7021 gagacggcgg ctaacgacga cgttctggcg atcctgatcg aggaaggcat cggcttcacc
     7081 atcctggctc ccaagcaagc gagccggtgg cgcgaggaag ggggccggtg gatccaggcg
     7141 gaagaggagc cggtcgaccc gagcatcccc tatcgctatc tccatcggga tggttcggga
     7201 cgatcgctga ccctgttctt ctacgacggc gagatcgccc ggaagatcgc cttcgagcgc
     7261 gccggatcgt cggccgaggg attcgtccga ctgttcgaag agagggccgg gtcgggccgc
     7321 cttgtccatg cggcgacgga cggagagacg tacggccacc atcacgtgtt ctccgatctc
     7381 gggcttgcct acgcgctgtt cgtggaggcc ggtgaacgcg atctcgaagt cacgaactac
     7441 gcggcctacc tcgccgatgc ggaggtcact cacgaagcga cgatctggcg tggagaagga
     7501 actgcgtggt cgtgtgccca cggagttgga cgctgggtac gcgactgcgg atgctcgacc
     7561 gggggcgacg aagggtggaa ccaacgttgg cgcggccccc tgcgcgccgg gctcgacatc
     7621 gtcaaacaca ctgccgacga ggtctacgag cgtctcggcg gcgcgctgct aagcgacccg
     7681 tggccggctc gtgacgacta cgtcaacgtc gtccttgggg cgcaggggct cgacgagttc
     7741 gtagcccacc actcctcggg tccctccgac gaaaccgttg cacggcagat cgagacgttg
     7801 ttggagatgc agaggaatgc catgtccatg tacacgagct gcggctggtt cttcaacgac
     7861 atctcgggga tcgagacgat ccaggtcctt cgttacgcgg ctcggacgtt ggacctcatc
     7921 gaggagctcg gccagcccac tccggaagcc tccttcctcg cgacgctgga acaagcccgg
     7981 agcaacgacc ggtcacaggg aaccggtgcg gatatctacc tcgcgctggt atcggccccg
     8041 atggcggcga gataaccctc cgcggcggat agccttaacc catgaagcgg accgcgcgat
     8101 cggctcggcg atcgcgcaaa ctgatggttg ttgtcttgtg cagcgcgctc gctttcgccg
     8161 cggggagcac gccgctcccc cacgcgaccg gccaggccgg gcccggctac ttcgccagcg
     8221 agaacgtcga gtggatcaag accctgcctc aggaaacgga cacatcggga ggcgtcttct
     8281 tcgacaagca cctctacgtc acgacggccc gggcactcac gatctacgac atctccgacc
     8341 cggaaaaccc tgcgatcacc ggcactcacc ccttaccgca gcaaccgcag ttctcggaag
     8401 aggaccccga cacgaacggc aagatcctgc tgatcggaac gctgggtatc ttgttcgtct
     8461 tcgacgtcga ggacaagacc aaccctcaga tcgtcgggca actcgatggt gccgacgagc
     8521 acacgatctc ctgtgtcctc gattgcaagt ggggctacgg atccgagggc gcgatcgtgg
     8581 atctgcgcga ccccacgaac ccgaagatgg cgggcgactg gggcgaaggc aacccggcaa
     8641 cgagcgggca cgacgtaacc gaggttgctc ccggattgat cgttacttca tcgaacccga
     8701 tgatgctcct tgacgcgcgc aaggatccga cgaaaccgaa ggtcgtggca ctcggcaagc
     8761 cccccgacaa tcgtttgatc cacggcaacc tgtggccggg aggaggcacc gacagattcc
     8821 tactcgtcgg aggtgaaacc accggcaatt gcggatccga gacttcgggg gcgttcatgg
     8881 tgtgggacgc ggcgaaggtc ggcgtaacgc gcaccttcac gatgatcgac gagctacgga
     8941 tggtgaacgg caacccggcc gagggcaacg cacccgccaa ttcgttctgc gcgcactggt
     9001 tcacgacgca cccgacctac aaggacggtg gcctggtcgc catggcgtgg tacgagcacg
     9061 gcacccggtt cctccaagtc acctcggagg gcaagatcaa ggaggtcggc tggttcctgc
     9121 cgatcggcgg agccacctcg gcgtcctatt gggtcaccga cgagatcgtt tacgccctcg
     9181 actaccagcg gggcctcgac atcctgcgct acaccggcaa gttctacgag ccgaccgggg
     9241 gcggaggcgg gacgggcggt ggcggtggcg gtggctccgg ttccggcgaa cagctcccgg
     9301 gtcccggagt tcgcatgagg atctcggatc gcacgcccgc ccgcgacgag gtcgttcgtt
     9361 tccgggtgct actgaggaag tgcccccgaa gccattaccg aacgctcgtc gagctccagc
     9421 gcaagagacg cgatcgtttc ttcacggtga ccgaggcgaa actgaaccgg aattgccggg
     9481 ccgtgatgcg ggtcccggcc gggttcgata aggccacgtt ccgagcgatc tggcccaagc
     9541 aggacgacga tcaccgagca ggtaggtcca agccgcagac cgtcacgccc cactgaagtc
     9601 tgcgcgacgg ccgaggccgc cccactaggg gttagcggcg cgatctcacc tttgcaaagc
     9661 gcagccgtgt cctgcgctgc gtgttcatcg ccacgccgaa ccctgcggac atcaagagtg
     9721 cggcccccca cagctcgacc ggtggaccgg gatccttgcg taacgacgtg aacgagtact
     9781 gctcctcgcg cgcgagagcc gactgcgcgt ccgcgtggag ctggtgcacc atcgccactt
     9841 gaggctgttc ctgttcctgt gccgcgaacg ccgcgttcgc ctgagcctgg gcttgagcct
     9901 gggtagccga agacacgttc accgggggcg gaggcggccc gatcgggatc agcgggacca
     9961 gggccacgaa acgatcgaac gggatcccga ggggcggttt gtcgggcggg atctcggcgc
    10021 acacgacacg cgtctgtgag acgggaggag tgccgatcag accggtggcc tgaaggtcga
    10081 cgtcgatctc tttgccggcg aggctctcgg ggcagcggta ggtcacatcg aactcgagcg
    10141 agctgtctgc ttgcaacagg acctggttgt agcggtcggg actgaccgac tggagcacac
    10201 cgtcccgctc gggatcgacc ttcaggccga cactcgactt cgtccgcact gcctcgacga
    10261 gctcgacgat cgccggcgcc atgttggcgt catcgatctc gtcctgttgg aggacgcaga
    10321 cgagtggctc gccggccggg acgtctgtgg aaccatcacg atcgcaatcg acaccccccg
    10381 ggggtgcgaa cgcaccggtc gcgtccgcca tacggcgaag gtccggtgtc gcgtcggggt
    10441 acagcgatag accaagttgc ttgaccccgt tggcgcgcag tgccgccgcc acggtttcga
    10501 actcggggat gtccggcttg gcgagctcgg actcgggcct cgtgacggtg ttgtcgctgt
    10561 ctgatgccga gtcctcccga ccgaacggct cgtcgccgac gtggagaacc acccgcagcg
    10621 cgttcttgcg gaagttcgcc tggatcccag gagggacgtc ccgagaagaa ggaccgggcg
    10681 gccacacgtc tgcaccgttg ccggtcgccg cctgccatag agcttcgagc tgcgcgtcgt
    10741 agacgcctcc gcctgccgcc gtcaatcctt cgatcgcatc ttccatctgc gcagtcgtcg
    10801 aacccacgtc cagcacacgc cggtagacgt agttgtccgt ctcggacttg ggcgggatgt
    10861 cgatgggata agagcggtat tcggcaagcc cgaactgaac ggccgcgccc gagctcgcca
    10921 gtccgcggta gatcgtttcg agagcctcgg ccgcctcctt gatgacctga gtcatgctcg
    10981 aagatgtatc cacgacgaag aacacgtcca gtggcgtcag cgtgcgcgga acaccgacgt
    11041 cgtatgtgac cgtccgcctc ttgccgatat ccagtcggac ggtcgtctgc ttcggcgtca
    11101 gcgtcgtctg gcgcgccacg tcgggcggcg cgacgtccag cagagagatg tcggggatct
    11161 cgatgatgtc ttcagggatg tcgaactcgg ggtcctcgcc cgtggggccg ctgtagatct
    11221 cgaccgttcg tgtcgtgtgc ccgtagaagc tcgggttgga cgtgcggtcg gcaaggaggc
    11281 ctcggatccc cgcgcgcggc gcgttcaggt cgacccagtt gagtaccgtc ggcgcccacg
    11341 cgtatacccc gcgaccggtc gacatgacga tgctctcccg gaagtcaccg tgcgcgaccg
    11401 aattcggagt a
//
LOCUS       DATMED010000238         3005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1724710, whole genome shotgun sequence.
ACCESSION   DATMED010000238 DATMED010000000
VERSION     DATMED010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724710"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..815
                     /locus_tag="VNP73_06160"
     CDS             60..815
                     /locus_tag="VNP73_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWL65542.1"
                     /translation="MAKLRAVVLAAGRGVRMGGNEPKTLLPLGDRQPLLYYILRGLKQ
                     AGIEDLMVVTGFKPQVVQEYVTEQWGEATFVFNARFASWGNFHTLRLALDQSPGMDVM
                     AVNSDIVVHPNVLKNVRETPGDLVLAVQQRYTLNEEDMRVKIDGNKVLAIGKHLPKRI
                     SHGEFAGVSLLRPDGAAAYLEIASDLEWQADTQGYYEDVFGKMLARVDAREAQVGQDE
                     YAEVDLPADVEQAVRVIDRHPDAWGVPAQTTPS"
     gene            821..1855
                     /locus_tag="VNP73_06165"
     CDS             821..1855
                     /locus_tag="VNP73_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWL65543.1"
                     /translation="MDLPQVFVSDASGDEAQAATELARTVVDLGIEVPFVFASTHGAG
                     VAAELEGPQAPTGSTQPPDQRWAADLGSAARRSGADGIVAIGGGRRLDIAKLAAARAG
                     LQFIAAPTQLSHDGICSPVAVVPNEAGTAESLGAVEPKAVFLSMPTLLRAPVRSVIAG
                     IGDLLANPLALRDWALAAERGLDEIDQRAWDLSVHAFELIEPHLDGDPSTMAKDVGFL
                     AVLGEALIQSGFAMISSGTSRPASGGEHEISHALDELFGGPAMHGEQVAFGSIISVAL
                     YGEDTHLFRKRLRRLGLPQDPGDLGLDTEAMVRVILEAPNTRPGRFTILENADLDEPG
                     ARRLIESIWS"
     gene            1869..>3005
                     /locus_tag="VNP73_06170"
     CDS             1869..>3005
                     /locus_tag="VNP73_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65544.1"
                     /translation="MNRAVMIGLDGAAWHLLDPLIEAGHMPRLGSLRESGAAGILKST
                     VPTYTPPAWTSAATGVNPGRHGVYGFIEGHAQHEQTELMHSGKIKSPTIWEVANAQGA
                     RVGVYNVPLTYPPRPLDGWMVSGMLTPGFGEQQRGFATWDRGGGEDLESKILGWTPDY
                     VLDLHANYEQDWRDDALAQRGLESVRQRKKVLEGLLETDPPDIVFSVLETPDRLQHVY
                     YRYLDPNDPEYDSPAARTIKPVLHACFAGMDEIVGLLHDYSDGGGVVVCSDHGFTAWE
                     VSVHTNALLQQWGYLQLKTSARVMQTGLARSAVPVAKRFLPRKLAREAKGRTFGAIDW
                     SQTRAFASPIPQQGIFVNLKGREPNGIVEHADLASLKEELAARFR"
BASE COUNT          568 a          949 c          974 g          514 t
ORIGIN      
        1 aaggatggga gactcctcat ggagcgccca ctgaagaaag acttggagtt ggagatctca
       61 tggcgaagtt gagagcggtc gtacttgcgg cgggacgagg cgtccgcatg ggtggcaacg
      121 aaccgaagac attgttgccc ctgggggaca gacagcccct cctctactac atcctgcgcg
      181 gcctaaaaca ggccgggatc gaagacctga tggtggtcac cggattcaag ccacaagtag
      241 tccaggagta cgttaccgag cagtggggtg aggcaacctt cgtgttcaat gcacgcttcg
      301 cctcgtgggg aaacttccac accttgcgct tggcgctcga tcagtcaccc ggtatggacg
      361 tcatggcggt caactcggac atcgtcgtgc atcccaacgt tctcaagaac gtacgcgaga
      421 cgccgggaga tctcgtcctg gcagtgcaac agcgatacac gctcaacgag gaagacatgc
      481 gcgtgaagat cgatggcaac aaagtgctcg ccatcgggaa acacctccct aagaggatca
      541 gtcacggcga gttcgcgggt gtttcgttgc tccgaccgga tggggcagcc gcctatctcg
      601 agatcgcatc cgatctcgaa tggcaagcgg atacgcaggg ctattacgaa gacgtcttcg
      661 gcaagatgct cgcaagggtc gacgcgcgtg aggcgcaggt cggacaagac gaatatgcag
      721 aggtagacct ccccgccgat gtcgagcagg ccgttcgggt catcgatcga catccggacg
      781 cgtggggagt gccggcccaa accactccga gctgaccgcc ttggatctgc cacaggtatt
      841 cgtttccgac gcgtcgggag atgaggcgca agccgcgacc gagctcgcac gaaccgttgt
      901 cgatctgggg atcgaggttc ccttcgtttt cgcctcgacg cacggcgccg gggtcgcggc
      961 cgagctcgaa ggcccccagg caccgacggg atccacgcaa ccaccggacc agaggtgggc
     1021 ggccgatctg ggctccgccg cgcggcgatc gggcgccgac gggatcgtgg cgatcggtgg
     1081 aggcaggagg ctcgatatcg ccaagctcgc ggctgcgagg gcgggcttgc aattcatcgc
     1141 ggcccccaca cagctctcgc atgacggcat ctgctctccg gttgcagtgg tgcccaacga
     1201 ggccggtacg gcggagagcc tcggtgcggt cgagccgaaa gccgtcttcc tctcgatgcc
     1261 gaccctgctc cgcgctccgg ttcgatcggt tatcgccggg atcggagacc tgctggccaa
     1321 cccgctcgcg ttacgggact gggctctagc ggccgagaga ggcctcgacg agatcgatca
     1381 gcgagcgtgg gacctgtccg tgcatgcgtt cgagctgatc gagccgcatc tcgacggcga
     1441 tccatccacg atggctaaag acgtggggtt cctcgccgtc ctcggtgagg cgttgatcca
     1501 gtccggtttc gcgatgatct cctccggcac ctcgcgcccg gcgtccgggg gcgagcacga
     1561 gatctcgcac gctctggacg agctgttcgg gggcccggcg atgcacggcg agcaggtcgc
     1621 tttcggatcg atcatctccg tcgctctgta cggagaagac acccatctgt tccgcaaacg
     1681 acttcgcagg cttggactcc ctcaggaccc cggcgatctc ggcctcgaca ccgaggcgat
     1741 ggttcgggtg atcctcgagg ccccgaacac gcggcccggg cgcttcacga tcctcgagaa
     1801 cgctgacctc gacgagcccg gggcgcggcg gctgatcgag agcatctggt cgtgagacga
     1861 tgccgcgcat gaatcgtgca gtcatgatcg gtctcgacgg agccgcgtgg cacctgctcg
     1921 acccgttgat cgaagccggg cacatgcccc ggctgggttc gctcagggaa tcgggagcag
     1981 ccggcatcct caagtcgacg gttcccacgt acacaccgcc cgcgtggacc tcggccgcga
     2041 cgggtgtgaa cccgggccga cacggcgtgt atgggtttat cgaaggtcac gcacaacacg
     2101 agcaaaccga attgatgcac tccggcaaga tcaagtcccc caccatctgg gaggtcgcca
     2161 acgcgcaggg tgcgcgcgtg ggcgtctaca acgtgcctct cacgtatccg ccccgcccgc
     2221 tggacggttg gatggtgtcg gggatgctga cacccggctt cggcgagcag cagcgaggct
     2281 tcgctacatg ggatcgcggc ggcggagagg atctcgagag caagatcctg ggctggactc
     2341 ccgactacgt gctcgatctt cacgccaact acgagcagga ctggcgagac gacgcgctcg
     2401 cgcagcgcgg gctcgaatcg gtccgacaac ggaagaaggt cctggagggg ctactggaga
     2461 ccgatccacc cgacatcgtc ttctcggttc tcgagacccc cgatcgcttg cagcacgtct
     2521 actaccgcta tctggatccg aacgaccccg agtacgactc ccccgcggcc cgcaccatca
     2581 agccggtact tcacgcctgc ttcgcaggga tggacgagat cgtcggattg ctccacgact
     2641 attccgacgg tggcggcgtt gtggtctgtt cggaccacgg attcaccgcg tgggaagtaa
     2701 gcgtgcatac gaacgcgttg ctgcagcaat ggggctatct gcagctgaaa acgagtgcac
     2761 gcgtgatgca gacgggacta gctcgctccg ccgtcccggt ggcgaaacgt ttccttcccc
     2821 gcaagttggc gcgcgaggcg aagggccgga cattcggcgc catcgattgg agccaaaccc
     2881 gggcattcgc ctcccccatc ccccagcaag ggatcttcgt gaacctgaaa ggccgcgagc
     2941 cgaacgggat cgtcgagcat gccgaccttg catccctcaa ggaggaactg gccgcgcggt
     3001 tccgc
//
LOCUS       DATMED010000239         4442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3637465, whole genome shotgun sequence.
ACCESSION   DATMED010000239 DATMED010000000
VERSION     DATMED010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637465"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VNP73_06175"
     CDS             <1..259
                     /locus_tag="VNP73_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65545.1"
                     /translation="TARGSARITRPNLEKAVAGFPLNVSLPEIDLSAIRLDQNALDMG
                     PFHLELPVIAEGMEYEALRVVDGDLHLTFTLNGDQLRLRNF"
     gene            complement(256..633)
                     /locus_tag="VNP73_06180"
     CDS             complement(256..633)
                     /locus_tag="VNP73_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65546.1"
                     /translation="MPTVLICDDVSELRALVRRALKDEPDIEVIGEAGDGQQAVEMAS
                     NLRPDVILLDVSMPVKDGLTALTEIRANCPESKIIMFTALNEALAREPAERRGADAYV
                     GKGALLTELAESIRSVTTPGKDG"
     gene            complement(705..2204)
                     /locus_tag="VNP73_06185"
     CDS             complement(705..2204)
                     /locus_tag="VNP73_06185"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL65547.1"
                     /translation="MEPSQTGISRELALITRWIAAASVALLFGAVSLGGGGTAGAAGP
                     DVEGSGGRAVAHHAGGAPIKSPAVLRKAPSAEMFRVGLNSSEPTLGVNEDNEVFYVAF
                     QTNTRVEVVKSSDEGKTWDIVSPKFPNGRNAQLLSLDPYVFVDDTEGSNRIFTIDLTV
                     ACSYLSFSDDSGKSWITNPLACGRPVNDHQTLFAGPPAISPTVGYPSVVYYCWNDIAS
                     SSCSKSLDGGITFTPTTPAFVGEDPNRDEGSLQGQDVRGFCGGLHGHGYVGEDGTVFV
                     PRGYCGTPELAISKDEGLTWTKATVSKKVPISGHEASVATDSKGNIYYSWVGDKDRLP
                     YLSISKDEGKTWSKPMMVGAPGLREANLPSLDVGAPGHVAIAYMGSENSPGMPFPEFG
                     ECTAIGACPEPDKYKKVSWNGYMTETHNALDNDPLFYSGTVNNKKDPLIRGTCGPGRC
                     KAVFDFIDIVISPKGETWAAWVDGCITVCPDPRATSNMGSDGVVSRLKY"
     gene            2370..3764
                     /locus_tag="VNP73_06190"
     CDS             2370..3764
                     /locus_tag="VNP73_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWL65548.1"
                     /translation="MGPSTAEIEHGRGRLLGLLFVVLAGFASLSVVLTALGPDTLASL
                     QLTPGAARWGVLVLTIGFIALVWEKERQYRHWTEVATHQQILIASFENRLRVVEGLLE
                     TSDRMNTPLALDDVIEVILESAVDLVGAAGGSIELYEGDKNEVSLSYEYSIDKKSADP
                     ERRLSMPLMEGRTMIGWLHISPSKTQSFERATTEALERFVVQAAHAVRKSQALARERA
                     GDAYREAANIVRSRFLATMSHELRKPVGAMRKISKTLSIHWDHLTEHEKVNFITELSD
                     NGHSMEGLLDRLLEAAKLEIENVVVHPVQHDVRRSIRKALMPFLAHSAGRLEVHLPDT
                     ELIAEVDPFVVDQTVSILVDNALRCTEGKVFIGARHLHGSLAIAIADEGPGLLGEKLG
                     LVLNPVSWQDDEMKTGDGLALHIVGTLVESHQGDIDVISDEAGTRVCVTLPEQAAGDQ
                     IRLEDPDEVIAPAE"
     gene            3837..4202
                     /locus_tag="VNP73_06195"
     CDS             3837..4202
                     /locus_tag="VNP73_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65549.1"
                     /translation="MATRPVVLLVDDDPTMRELLSDMFTLHGGFRIGGVAADGFEAAM
                     LAADLHPDVVVLDFFMPRWDGSKAAEFIRERCPESRIIAFSAVLTDAPQWGDTFLIKN
                     DVDKLIPLCEEMCAPYSHN"
BASE COUNT          809 a         1378 c         1392 g          863 t
ORIGIN      
        1 gaccgcacgg ggctcggccc ggatcactcg tccgaacctc gagaaggcgg ttgccggctt
       61 tccgctgaac gtcagcttgc ccgagatcga tctgagcgca atccggctcg accagaacgc
      121 gctcgacatg ggcccgttcc atctcgagtt acccgtgatt gccgagggca tggagtacga
      181 ggcgctacgc gtagtcgacg gcgaccttca cctgacgttc accctgaacg gcgaccaact
      241 ccgacttcgt aacttctaac cgtctttccc aggtgtggtc acggagcgga tgctctctgc
      301 gagttcggtc aggagtgcac cttttcccac gtaggcatcg gcgccgcgac gctctgccgg
      361 ttctcgcgcg agcgcttcat tcaaggcggt gaacatgatg atcttggact cggggcagtt
      421 ggcccggatc tccgtcaggg ccgtgagccc gtccttcacg ggcatgctga catcgagcag
      481 gatgacgtcg ggacgaaggt tcgatgccat ctctactgct tgttgaccgt cgccggcctc
      541 gccgatcacc tcgatgtcgg gctcatcttt tagagcgcgt ctcaccagag cccgtaactc
      601 actaacgtcg tcgcagatca gaactgtggg catgacgttc ttttctaaat ctcccgcctg
      661 gaatgggggc gcattgtgca gaaagaacgg ttggtgggga aaggttagta ctttaggcgg
      721 cttacaactc catcggagcc catgttgctc gtggctctgg ggtccgggca gaccgtgatg
      781 catccatcaa cccaagcagc ccaggtctcg ccctttggcg agatcacaat gtcgatgaag
      841 tcgaagaccg ccttgcagcg gcccggcccg cacgttcctc ggatcaacgg gtccttcttg
      901 ttgttgaccg tgcccgagta gaagagcggg tcgttatcaa gagcgttatg ggtctcggtc
      961 atatagccgt tccacgacac cttcttgtac ttgtcgggct cggggcaggc tccgattgcc
     1021 gtgcactcgc cgaactcggg gaacggcatg ccgggtgagt tctcggatcc catgtaggcg
     1081 atcgcaacgt gtccgggcgc tccgacgtcg aggctcggca ggttcgcctc ccgaagaccc
     1141 ggcgcgccga ccatcatcgg cttgctccac gtcttgccct cgtccttaga gatcgagagg
     1201 tatgggagcc ggtccttgtc acccacccac gagtaataga tgttgccctt gctgtcggtc
     1261 gcgaccgagg cttcgtgccc ggagatcgga accttcttgg acacggtcgc tttcgtccaa
     1321 gtgagcccct cgtccttgct gatcgcgagc tcgggagtgc cgcagtatcc acggggtacg
     1381 aacactgttc cgtcttcgcc gacgtagcca tgaccgtgaa gtccgccgca gaagccccga
     1441 acgtcttgtc cctgcaagct gccttcgtcg cggttggggt cctcgcccac gaaggcgggt
     1501 gtcgtaggag tgaaagtgat gccaccgtcg agcgatttgc tacacgagga cgatgcgatg
     1561 tcgttccagc agtagtagac gacgcttgga tagccgaccg tagggctgat ggccggggga
     1621 ccggcgaaca gggtctggtg gtcgttgacc ggtcgtccgc acgcaagtgg gttcgttatc
     1681 catgacttgc ctgagtcgtc ggagaaggag aggtaggagc acgcgacggt caggtcgatc
     1741 gtgaagatcc ggttcgagcc ttcggtgtcg tccacgaaga cgtaggggtc gagcgagagt
     1801 agttgcgcgt tgcgtccgtt ggggaacttc ggcgagacga tgtcccacgt cttgccctcg
     1861 tccgagctct tgacgacctc gacgcgggtg ttcgtttgga aggcgacgta gaagacttcg
     1921 ttgtcctcgt tcactccgag ggtcggctcg gacgagttca gcccgacacg gaacatctcg
     1981 gccgaaggag ctttccgaag cacggcgggg gacttgatcg gggcgccacc ggcatgatgg
     2041 gcgaccgccc ggcccccgga cccctcgacg tctggtcccg cggccccggc cgttccaccg
     2101 ccgccgagag agaccgcgcc gaaaaggagg gcgacgctgg ctgcggcgat ccacctcgtg
     2161 atcagggcta gctccctcga tatccccgtt tgagaaggct ccaccaccgc cttcagggtc
     2221 tattcagggg gttcggcaga ttttgaggtt tccctcccat ttcgtggggg aactactttc
     2281 tgtagccgac taaagacagt gagtagttaa gccgagtaag gtaatagccc atttgggcta
     2341 cggagaggtt agttcttaca ggaggagcgg tgggcccaag cacggcagag atcgaacacg
     2401 gacgcggccg attgctcggt ctgctgttcg tggtgctggc aggattcgcc agcctctcgg
     2461 tcgtcctcac cgccctcggt cccgacaccc tcgcttcgct ccagctgacc cccggggccg
     2521 cccgttgggg cgtcctggtc ctcacgatcg ggttcatcgc actcgtgtgg gaaaaagagc
     2581 gtcagtaccg ccactggacg gaggtggcca cccaccaaca gatcctgatc gcctcgttcg
     2641 agaaccggct ccgagttgta gagggccttc tcgaaacgag cgatcgcatg aacacaccgc
     2701 tggctctgga cgacgtgatc gaagtgatcc ttgaatccgc ggtcgacctc gttggggcgg
     2761 ccgggggctc gatcgagctc tacgagggag ataagaacga ggtctcgctc agctacgagt
     2821 actcgatcga caagaagagt gcggatcccg agcggcgcct gagtatgcct ctgatggagg
     2881 ggcgcaccat gatcggctgg ttgcacatct ctccttcgaa aacgcagagc ttcgagcggg
     2941 ccacgaccga ggcgctcgag cgtttcgtgg tccaagccgc gcacgcggtc cgcaagtccc
     3001 aagctctcgc ccgcgagcga gccggcgacg cttaccgcga agcggccaac atcgtccggt
     3061 cccgattcct cgccacgatg tcgcacgagc tgaggaagcc ggtgggggcc atgcgcaaga
     3121 tctcaaagac gctatcgatc cactgggatc acctgaccga gcacgagaaa gtgaacttca
     3181 tcaccgagct gtcggataac gggcacagta tggaggggct cctcgaccgg ctgctcgagg
     3241 cggcgaagct cgagatcgag aacgtggtcg tgcacccggt gcaacacgac gtccgtcgtt
     3301 cgatccgaaa ggctctcatg ccgttcctgg cgcattccgc cgggcggctc gaggtccacc
     3361 tcccggatac cgagttgata gcggaggtcg accccttcgt cgtcgatcag acagtctcga
     3421 tcctcgtcga caacgcgctg cgctgcaccg agggaaaggt cttcatcggt gcccgccacc
     3481 tgcacggctc ccttgcgatc gcgatcgcag acgagggtcc aggtctgctg ggcgagaagc
     3541 tcggcctcgt actgaacccc gtcagctggc aggacgacga gatgaagacc ggcgacggcc
     3601 tggcgctcca catagtcggc accctggtgg agagtcatca gggcgacatc gacgtgatct
     3661 cggacgaggc cggaacccgg gtgtgcgtga cgctgcccga gcaagcggcc ggcgaccaga
     3721 tccgtctgga agaccccgac gaggtcatag ccccggcgga atagccccgc agctccagag
     3781 gaatagccga aacgggctat tgcccccgaa cctcccaagc acgaccattc caaacgatgg
     3841 ctactcgacc cgttgttctg ctcgtagacg acgatccgac gatgcgcgag ctcctgtccg
     3901 acatgttcac gctgcacggc gggttccgga tcgggggagt ggccgcggac ggattcgagg
     3961 ccgcgatgct cgctgccgac ctgcaccccg acgtcgtcgt gctcgacttc ttcatgccgc
     4021 gctgggacgg gtcgaaggct gccgagttca tccgggagag gtgccccgag tcgcggatca
     4081 tcgctttctc tgccgttctg accgacgccc cccaatgggg cgacacgttc ctgatcaaga
     4141 acgatgtgga caagctcatc cctctgtgcg aggagatgtg cgccccctat tcgcacaact
     4201 aaccccttag tcacaaacgc gctttcacgc gcgctccata ccttgctcgc ctaggcttct
     4261 ctgtgatgat cgaccagcga agctcgctca tcgctatgtg gatcgacgct ctcgagcgcg
     4321 ccggttgggc agcgttcgtc gtcgatgaga agtatcggct cgcgtgggtc tcgaacgagc
     4381 acaaagactt cctcgacgaa cacgacgacg aggcggtcgg tgtcggtagg cactgcttgc
     4441 tc
//
LOCUS       DATMED010000240         5923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1728466, whole genome shotgun sequence.
ACCESSION   DATMED010000240 DATMED010000000
VERSION     DATMED010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728466"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNP73_06200"
     CDS             <1..278
                     /locus_tag="VNP73_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65550.1"
                     /translation="QVAAVFSFEGFNPLTRNDVGADPKARKTGTRTIEGFQSECFTVP
                     QADSKERAEVCTSGDTGLITFWDSAEGSWRLERVGPARMFRLPFKPR"
     gene            complement(283..678)
                     /locus_tag="VNP73_06205"
     CDS             complement(283..678)
                     /locus_tag="VNP73_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65551.1"
                     /translation="MELRIVLVDDNPEYRFLLRQIIERGTTFSVVGEGWNGEEAIELV
                     DKLEPHVVVMDIMMPVLGGVEATHVIKERHPGVRVLALTSSMDESHAEDMSGAGVDGY
                     FLKNDPVEKLLGQLDSIELAARSSPLRQH"
     gene            complement(789..1991)
                     /locus_tag="VNP73_06210"
     CDS             complement(789..1991)
                     /locus_tag="VNP73_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65552.1"
                     /translation="MRSIRIAGAIALLAAMALPATIANAAANMSKNVSVIEEIKYWGG
                     SHLTFGGDYAYAAQWNGRGEKPKVGGVHIMDISGHPKEVGFFKCPGEDVDVAFVEPGV
                     VAVGHHAAVCNPFESGEDDAGLYTVDVSDPKNPTMLGEVNLPRDVDAVDRTGRQNHSI
                     SKYPGKPIIYTNAGGLPSNGLMLTHMIDVSNPEKPKVVGEFRVPAPSTGCHDFSFHFD
                     KRGKFGFCSGLGGTSIWDVSDPLAPTVLTHIVNPLIQFSHYAVASADGKILAINDENV
                     SANECVPEETPTGAVWFYDISDIENPQMISYFSPPRGASPIGSFETPDGPCTSHDFNW
                     AGNRTVVMPWYTGGFSVIDVKEPAAPKEIAFYQPEGGSMWSAHYYRGRIYTNDQLLGF
                     TALEVAGL"
     gene            2132..2575
                     /locus_tag="VNP73_06215"
     CDS             2132..2575
                     /locus_tag="VNP73_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016714208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWL65553.1"
                     /translation="MPNAIELLETDHKKVKKLLEQGDDTTENAVKTRTELLGKIKTEM
                     EIHEAIEEEIFYPALKEHPKAKEIVLEGYEEHHVVDKILGELGDTSVEDERWGAKFSV
                     MKENIEHHIEEEEGDMFKRARDIFSKEELETLGERMEARKKELEA"
     gene            complement(2572..5478)
                     /locus_tag="VNP73_06220"
     CDS             complement(2572..5478)
                     /locus_tag="VNP73_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWL65554.1"
                     /translation="MTDITAERPLDTCELPGLEDLCLGVTGAGSQPPADAADGDGQAP
                     PQEAAPPGTGEWAIVESPNVSSEPNVLTAVTCPTPSDCWAVGRASDGLGSTVTMSWDG
                     SSWVTVPSPNIEDARANILSDVTCVTPSDCWAVGAYVTEGLQVRTLILQWDGVSWEIV
                     NSPDIGVDPWNGLNSVECISASECWAVGFNTTTGTAKTLILKWDGTKWDVHDSPNAST
                     KGNVLHGVTCLSGSDCWAVGSYTGDDATKHTLTMRWDGNVWEVVGSADNDAAQENVLS
                     GVTCVSDSDCWAVGHSFTGSARQTLIERWDGAAWTIVGSPNAIGPIDTYLSSVECASA
                     TECWAVGYSNDTVTIDQHFIARWNGVLWTPATLPDPVGTQASVLHDVACLSASDCWSV
                     GSFTAGTNRSLLNRWDGTTWESVPPPRVSLEEQSHGYLHDVTCVDASDCWSVGFYFFG
                     QVARSLIMHWDGESWDVVESPNVGLDRNNYLDSITCTSTSNCWVVGRHTTTLGVAGQT
                     LILRWDGEAWSIVPSFPADTSAAQSNALTGVSCSSESECVAVGFSSIEGGRYQALALH
                     WDGTSWLPGLVRLREDAETFYVPSDILYDVSCPAPSECLAVGAHWTGTVYQTLVDHWD
                     GTTWTPVTSPNTAPDRGNILSSVTCVSASDCWAVGSADDYEQGLIEHWDGTSWSIVDS
                     PQTGNILSAVSCLSATECWAAGPYYTPHPPAKTLLVRWDGGSWSKFSTPNASATQSNN
                     LAGIACASSSDCWAVGQYFVPGRTQTLTLRYGPKREDPLPEASSLAFTESCATAGQYS
                     DETRFEARLTDSVGDPLSGAPLTFTLIGEGLSREFTGTTDADGIAGIDPALVDRPGSY
                     ELTVRFAGDAGYLESATSRVFLVQKEDTVVDLRVLGQGSNRSLSARLSDLDSPADGLA
                     GRTISFYANSELIGSAVTDANGVAQIAIPAGHRGAKRTFGAAFAGDDFFLPSAD"
     gene            complement(5665..>5923)
                     /locus_tag="VNP73_06225"
     CDS             complement(5665..>5923)
                     /locus_tag="VNP73_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65555.1"
                     /translation="ILPTFLEERYRRASIAARIEAIDVYATSGSFIASLESGKKAFRK
                     HVVGTYLSRPHIPSADSSRAIPELWLEASGDEESEASAPSG"
BASE COUNT         1160 a         1866 c         1828 g         1069 t
ORIGIN      
        1 atcaggtcgc agccgtgttc tccttcgaag gattcaaccc cctgacgagg aacgacgtgg
       61 gggccgatcc caaggctcga aagaccggta cgcgcacgat cgagggcttc cagtccgagt
      121 gcttcaccgt cccacaagcg gattccaagg aacgcgccga ggtatgcacg tccggtgaca
      181 ccgggttgat caccttctgg gattcggccg aaggaagctg gagactcgaa cgggtgggcc
      241 cggcccggat gttccggctg cctttcaagc ctcgctagga atctagtgct gccgaagagg
      301 ggacgatcgg gcggctaact cgatgctgtc gagttggccg agaagcttct cgacggggtc
      361 gttcttgagg aagtacccgt ccacgccggc tccggacatg tcctcggcat gggactcgtc
      421 catggagctg gtcaatgcga gaacacgtac ccccgggtga cgctccttga tcacgtgcgt
      481 ggcctcgacg ccgccgagga ccggcatcat gatgtccatc acaactacgt gaggctcgag
      541 cttgtcgacg agttcgatcg cttcctcacc gttccaccct tcaccgacaa cggagaatgt
      601 cgtccctctc tcgatgatct gtctgagtag aaaccggtat tccgggttgt cgtccacgag
      661 cacgatccga agttccatca gtcggccgcc gttctatcgc ggactgcgcg ccaccgcgca
      721 gacgtcgtca cctttggtta tcggccgatg cgctcgaaac ctgaagccat tctgacggag
      781 tcgcggcgct agagtcccgc gacctccagc gccgtgaatc cgagcaactg gtcgttcgtg
      841 tagatccggc ccctgtaata gtgcgccgac cacatgctgc cgccttccgg ctggtagaag
      901 gcgatctcct tcggggccgc cggctccttc acatcgatga cgctgaagcc acccgtgtac
      961 cacggcatca caaccgtccg gttaccggcc cagttgaagt cgtgcgacgt acagggtcca
     1021 tccggagtct cgaatgagcc gatcggcgac gctccgcgtg gcggcgagaa gtacgagatc
     1081 atctgcggat tctcgatatc cgatatgtcg tagaaccaca cggcgccggt aggcgtttcc
     1141 tccggcacgc actcgttcgc gctgacgttc tcgtcgttga tagcgaggat cttgccgtcg
     1201 gcgctcgcca ccgcgtaatg cgagaactgg atcagaggat tcacgatgtg ggtgaggacc
     1261 gtcggtgcca aggggtcgga cacatcccag atggacgtgc caccgaggcc cgaacagaag
     1321 ccgaacttgc cgcgcttgtc gaagtggaac gagaagtcgt ggcaacccgt cgagggggcg
     1381 ggcacacgga actcacccac gaccttcggt ttctccgggt tggagacatc gatcatgtga
     1441 gtgagcatca gaccgttgct gggaagtccg cccgcgttcg tgtagatgat cggcttaccg
     1501 gggtacttcg agattgagtg gttctggcgg ccggtcctgt cgactgcgtc cacatcgcgc
     1561 ggaaggttga cctcgccgag catcgtcggg ttcttcggat cgctcacgtc caccgtgtaa
     1621 agccccgcat cgtcttcacc ggactcgaac gggttacaga ccgcggcgtg gtggccgacg
     1681 gcgacgacgc ccggctccac gaaggcaacg tcgacgtctt ccccggggca cttgaagaaa
     1741 ccgacctctt tgggatgccc ggaaatatcc atgatgtgca cgccaccgac cttgggtttc
     1801 tcaccgcgcc cgttccactg tgcggcgtac gcgtaatcac cgccgaaagt caggtggctg
     1861 ccaccccaat acttgatctc ttcgatgacg ctcacattct tggacatgtt ggcagccgcg
     1921 ttggcgatcg tcgccggcag cgccatcgca gcgaggagcg ctatcgcgcc cgcgatcctg
     1981 atggatctca tggtggtccc cctagttcag ttgtctccca gagggttcgt cccaccaggt
     2041 ggttttcctc ccgcgccccg tgatgtgaat ttctcggatg gctggggaat aacccctctc
     2101 aaccggatta cagccccaaa aaggagtacg gatgccgaac gccatcgagc tgttggaaac
     2161 ggaccacaag aaggtcaaga agctcctcga gcagggagac gacacgacgg agaacgccgt
     2221 caagacccga acggagctgc tcggcaagat caagaccgag atggagatcc acgaagcgat
     2281 cgaagaagag atcttctatc ccgccctcaa ggagcacccc aaggcgaaag agatcgtgct
     2341 cgagggatac gaggaacatc atgtcgtcga caagatcctg ggcgagctcg gtgatacctc
     2401 cgtggaagac gagcgatggg gtgccaagtt ctccgtgatg aaagagaaca tcgagcatca
     2461 catcgaggag gaagagggcg acatgttcaa gcgagcgcgc gacatcttct ccaaggaaga
     2521 gttagagacg ctcggcgaga ggatggaagc gaggaagaaa gaactcgagg cctagtcggc
     2581 cgaaggtagg aagaaatcgt cgcctgcgaa cgcggcccca aaggttctct tggcaccgcg
     2641 gtgcccggcg ggtatcgcga tctgcgcgac gccgttagcg tccgtcacgg ccgagccgat
     2701 gagttcggag ttcgcgtaga aactgatggt ccgccccgca agaccgtccg ccggtgagtc
     2761 caggtccgaa aggcgggcgc tgaggctcct gttgctacct tgccccagaa ccctgaggtc
     2821 gacgaccgtg tcctccttct ggaccaggaa caccctgctg gtcgcggact ccagatagcc
     2881 ggcatcgccg gcgaaccgaa cggtcaattc gtaggagccc ggtcgatcca ccagcgccgg
     2941 atcgatcccg gcgatcccat ccgcgtcggt tgtgccggtg aactctcgac tcaacccctc
     3001 accgatcaga gtgaatgtga gcggcgctcc cgataacgga tcaccgaccg aatcggtgag
     3061 ccgcgcctcg aagcgcgtct cgtccgagta ctgtccggcc gtcgcgcagc tttcggtgaa
     3121 ggcgaggctc gaggcctcgg gcaacgggtc ttcgcgcttc ggtccgtacc gcaacgtcag
     3181 cgtctgggtg cgtcccggca cgaagtactg gccgaccgcc cagcaatcgg acgacgacgc
     3241 gcacgctatt cccgcaaggt tgttggactg agttgcactc gcattcgggg tagagaactt
     3301 gctccacgat cccccatccc aacgcacgag caacgtcttg gccggcggat gcggcgtgta
     3361 gtacgggcct gctgcccagc actcggttgc cgacaaacaa gagacagcgc tcaagatgtt
     3421 tccagtttga ggcgaatcaa cgatgctcca ggaggtccca tcccagtgtt cgatgaggcc
     3481 ctgctcgtaa tcgtcggcac tgccgaccgc ccagcagtcg ctcgccgaca cgcaggtaac
     3541 gctcgacagg atgttgcccc tatcgggagc cgtgttgggc gaagtgaccg gcgtccaggt
     3601 agtcccatcc cagtgatcga caagcgtttg gtagacggtc ccggtccaat gagccccgac
     3661 cgccaaacat tcagatggtg ccgggcacga aacgtcatac aagatgtcgc tcggtacgta
     3721 gaaggtttct gcgtcctccc gcaaacggac gagcccgggt agccacgatg tgccatccca
     3781 gtgaagcgca agtgcttgat agcggccgcc ttcgatcgag gagaagccca cggccacaca
     3841 ctccgactcg gacgagcacg acacgcccgt gagagcgttc gactgtgccg cgctggtgtc
     3901 cgccgggaac gaaggaacga ttgaccatgc ctcgccgtcc caccgcagga tcagtgtctg
     3961 acctgccaca ccaagtgtcg tcgtatgtcg cccgacgacc caacagttgg atgtggatgt
     4021 gcacgtgatg ctgtcgaggt agttgttcct atccaggccg acgttcggcg actccacaac
     4081 gtcccacgat tcgccatccc aatgcatgat caggctgcga gcgacctggc cgaagaagta
     4141 gaagccgacc gaccagcaat cggaggcatc gacgcaggtc acgtcgtgca ggtagccgtg
     4201 gctctgctct tccaggctga ctcggggtgg aggcaccgac tcccacgtgg tcccgtccca
     4261 cctatttagg agcgagcggt tcgtgccggc cgtgaaagag ccaaccgacc agcaatccga
     4321 cgccgacaag caggcaacgt cgtgcaggac gctcgcttgg gtccccaccg ggtcgggcaa
     4381 agtcgcgggt gtccagagaa caccgttcca gcgcgcgatg aaatgctggt cgatcgtgac
     4441 ggtgtcgttc gagtaaccga ccgcccagca ctcggtcgcc gacgcgcatt ccacgctcga
     4501 caggtaggta tcgatcggac cgatggcatt tggcgagccc acgatcgtcc acgcggcccc
     4561 atcccagcgt tcgataagcg tctggcgggc gctcccagtg aaggaatggc cgacggccca
     4621 gcagtctgag tcggacacgc acgtcacgcc ggagagaacg ttctcttgcg cagcatcgtt
     4681 gtccgcggac ccgacgacct cccaaacgtt tccatcccag cgcatcgtca gggtgtgctt
     4741 cgtggcgtcg tcgcctgtat aggagcccac cgcccagcag tcggatcccg ataggcacgt
     4801 aacaccatgc aatacgttgc ccttcgtgct cgcgttaggc gagtcgtgga cgtcccactt
     4861 ggtgccgtcc cacttcagga tcagggtctt tgccgtgccg gtggtcgtat tgaagccgac
     4921 ggcccaacac tccgacgcgg agatgcactc aacgctgttc agtccgttcc agggatcgac
     4981 tccgatgtcg ggcgagttga cgatctccca cgagacgccg tcccactgaa ggatgagcgt
     5041 ccggacctgg agaccctcgg tgacgtaggc accaacggcc cagcagtccg acggcgtcac
     5101 gcacgtcacg tcggaaagga tgttggcgcg cgcatcttcg atgttgggcg acggaaccgt
     5161 gacccacgaa cttccgtccc acgacatcgt caccgtggag ccgagaccgt cgctcgcgcg
     5221 gcccaccgcc cagcaatccg acggtgttgg gcacgtcacc gctgtaagga cgttcggctc
     5281 ggaactcacg ttgggcgatt cgacgatcgc ccattccccc gtgcccggag gggccgcttc
     5341 ctgaggagga gcctgaccgt cgccgtcggc ggcgtccgcc gggggttgcg accccgcccc
     5401 cgtcacgccg agacatagat cttcgaggcc cggaagctca caggtatcca aaggccgttc
     5461 ggcggtgatg tcggtcatct cttggagcga tgcaagatgg cgacgttccg gaatgttgtc
     5521 gtgcgcggga gtagacgcgc tcgcccgagg ggcgcctata agccccggaa ccaccaggaa
     5581 cagggccgcc atacggcgtc ggtgtgcttg cactgctcaa catttcgacg ccccgagggc
     5641 gacacctgcg aaaaagctgg atcgtcagcc ggaaggcgcg ctggcctcgg actcctcatc
     5701 gccgctcgcc tcgagccaca gttccgggat ggctcgagac gagtcggcgg aggggatgtg
     5761 ggggcgactg aggtaggtgc ccaccacatg tttcctgaag gccttcttgc ccgactcgag
     5821 gctcgctatg aacgagcccg atgttgcgta gacgtctatg gcttcgatcc gggccgcgat
     5881 ggaagcgcgt ctgtacctct cctcgaggaa cgtcggcagg atc
//
LOCUS       DATMED010000241         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_193481, whole genome shotgun sequence.
ACCESSION   DATMED010000241 DATMED010000000
VERSION     DATMED010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193481"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VNP73_06230"
     CDS             <1..1011
                     /locus_tag="VNP73_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HWL65556.1"
                     /translation="MHLSDDQGWRLWIDDWPNLASIGGSTEVGGGPGGYYTKDDYREI
                     VRYAQDRFITLIPEIDVPGHTNSAEVSYSELNSCRPGRVPSHRLLPEWEDEPFYTGTG
                     VGFSALCVHDEITYEFMEDVIREISKITPGPYFHVGGDEAFQTELPDYVMFMDRIQEM
                     VHDNGKTMMGWAEVARANAAPGSIAQNWSTATGGGSGGDLARMAVAKDMKVVMSPANR
                     IYLDMKYAPGVPPDLGLTWAGTVEVMRAYDWNPGAHLTGVTDDNIIGVEAPLWTETVE
                     DIKDAEMLSFPRLPGVGEIGWTPQQGRVWTEYRERLANQARRWAVMDINYYRSPQVDW
                     VD"
     gene            1105..>2153
                     /gene="dinB"
                     /locus_tag="VNP73_06235"
     CDS             1105..>2153
                     /gene="dinB"
                     /locus_tag="VNP73_06235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWL65557.1"
                     /translation="MRDTASILHADVDAFYASVEQRDDPRLRGRPVIVGAGVVLAASY
                     EAKAHGIRTAMAGSRARRLCPDAVVVSPRMSAYSEASKALFEVFDRTSALVEGLSIDE
                     AFLDVGGLWRLAGSPTAIAERLRREVLEEVGLPVSVGVARTKFLAKVASGVAKPDGLL
                     VVPPDRELAFLHPLPVEQLWGVGVVTSGKLRREGITTVAQVAQLAERTLVRLLGVATG
                     RHLHALAHNRDPRPVQVGRRRRSMGAQRSIGRKPKSAASVDAVLVSLVDRLSRRLRRA
                     HRVCRTVVLRLRFDDLSRATRSRTLSEATAQTQQILEVARTLLKEATPLIHSQGLTLV
                     GVSLTNLTNDGAIQLA"
BASE COUNT          386 a          684 c          701 g          382 t
ORIGIN      
        1 atgcacttgt ccgacgacca gggttggcgg ctatggatcg acgactggcc gaatctcgca
       61 tcgatcggcg gaagcaccga ggtgggtggc ggccccggcg gctactacac caaggacgac
      121 taccgcgaga tcgttcgata cgcccaagat cgtttcatca ccttgattcc cgagatcgac
      181 gtgcccggac acaccaactc ggcggaggtc tcttattccg agctcaactc ctgtcggccg
      241 ggccgggtcc cgtcccaccg cctcctcccc gaatgggaag acgaaccctt ctacacggga
      301 acgggcgtgg gcttcagtgc cctgtgtgtg cacgacgaga tcacctacga gttcatggaa
      361 gacgtcatca gggagatctc aaagatcacg ccgggcccgt acttccacgt cggcggtgat
      421 gaggccttcc agacggaact tcccgactac gtcatgttca tggaccggat tcaagagatg
      481 gtgcacgaca acgggaagac gatgatgggg tgggctgaag tcgcccgagc gaacgcggct
      541 cccggttcga tcgcacagaa ctggagcacc gccaccggcg gaggctccgg aggcgatctc
      601 gctcgcatgg ccgtggccaa ggacatgaag gtcgtcatgt cgcctgccaa ccgcatctac
      661 ctcgacatga aatacgcgcc tggtgtgccc ccggatctag ggctcacatg ggcaggcacg
      721 gtcgaggtca tgcgcgccta cgactggaac cccggcgcgc acctcaccgg tgttaccgac
      781 gacaacatca tcggtgtcga ggcgccgcta tggaccgaga cagtcgagga catcaaggac
      841 gccgagatgt tgtcgttccc gcggctgccc ggcgtcggtg agatcggttg gactccgcag
      901 caggggcgcg tatggacgga gtaccgggaa aggctggcga accaggcgcg ccggtgggcg
      961 gttatggaca tcaactacta ccgatcgcct caggtggact gggtcgacta gatcgccccg
     1021 cgaacacgcc cgcgggcatg actcggctct gtccgcgggc gtgcccccta tcctcgactt
     1081 gcgaggtatg cgaacatatg ttcgatgagg gacacggcct cgatcctgca tgccgacgtc
     1141 gacgcgttct acgcctcggt cgagcagcga gatgaccctc ggctgcgtgg tcgtccggtc
     1201 atcgtcggag cgggggtcgt cctagccgcg agttacgagg ccaaggcgca tggcatccgc
     1261 accgccatgg ccggctcacg agcacgccgg ctgtgtccag atgccgtcgt ggtgtcgccc
     1321 cgtatgtccg catactccga ggcgagcaag gcgctgttcg aagtctttga ccggacatcc
     1381 gccctcgtcg agggactgtc tatcgacgag gccttcctcg acgtgggcgg gctctggcgg
     1441 ctggccggtt cgccgaccgc aatcgccgag cgattgcgac gcgaggtgct cgaagaggtc
     1501 ggactcccag tctcggtggg tgtggcgagg acgaagttcc tcgcgaaggt ggcgagcggc
     1561 gtggcaaaac ccgacggcct gcttgtcgtg ccgccggaca gagagctcgc gtttctgcat
     1621 ccgctgcctg tcgaacaact atggggcgtg ggagtcgtca cctccggaaa gcttcggagg
     1681 gaggggatca cgacagttgc tcaggtagct cagctcgccg agagaacgct ggttcggctg
     1741 ctcggggtag cgaccggccg ccacctccac gccctcgctc acaatcgcga cccgcgcccc
     1801 gttcaggttg gccgccgtcg acgctcgatg ggtgcgcagc gatcgatcgg ccgcaaaccg
     1861 aagtcggccg cctcggttga cgccgttctc gtctcgctcg tcgaccgcct ctcccggcgg
     1921 ttgcgtaggg cgcatcgagt gtgtcggacc gtcgtgcttc ggttgcgttt cgacgatctc
     1981 tcgcgagcaa cacgatcgcg cacgctatcg gaagcaaccg cccagactca gcagatcctc
     2041 gaggtggcta ggacgttgtt gaaggaggcc actcctttga tccacagtca gggcctgacc
     2101 ctcgtgggag tgtcgctcac gaacctgact aacgacggcg caatccaatt ggc
//
LOCUS       DATMED010000242         5364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5556895, whole genome shotgun sequence.
ACCESSION   DATMED010000242 DATMED010000000
VERSION     DATMED010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5556895"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..146)
                     /locus_tag="VNP73_06240"
     tRNA            complement(73..146)
                     /locus_tag="VNP73_06240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Val,seq:tac)
     gene            complement(185..1879)
                     /locus_tag="VNP73_06245"
     CDS             complement(185..1879)
                     /locus_tag="VNP73_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HWL65558.1"
                     /translation="MKTAVVMTAVLAVIPTFVVGPGTAHAQEYMRGIDVSHHQGEIDW
                     NEVANSGHVFAFHKATEGATFIDDQYAGNRAEASAASVPFGAYHFARPNGETIEAAEA
                     DAEGEAEHFLEVAEPASGDLLPVLDLEATGGLPSDRLIAWTQVWVDAVAEAIDARPLI
                     YTSPNFWKTSLADTTTFADQGFPLWLAHYTSASSPSTPASNWGGHGWAFWQWTNCATI
                     PGISGCVDGDKFAGSDLTPYTVPGAPEPEPTPDDATPPANESPPEISGETEVGRTLNA
                     TSGSWSGSEPLAYSYAWHRCDADGSSCSPVLNGTEPTYRLVPADYQHTMKVTVTATNS
                     AGSSSSESSPSGVVTDSTDPEEPRMIKPRREQTLDTRLTIEWEASESQAHYAVRYLPT
                     VRGGRFRDYKRLVDDTTRTRTTLRAETGATYCFSARATDQAGNRSPWSDPRCTTVPFD
                     DRDLRRSSSWRTTSNRSAFLRTLTTTTERGASLDARNVSVREAHVVAQTCRRCGRIAV
                     MLRGRRIGTVDLRSRRTRTAVIRIKRFTSIREGDVSLVVVSDDAPVRIDGLALTVE"
     gene            complement(1898..3235)
                     /locus_tag="VNP73_06250"
     CDS             complement(1898..3235)
                     /locus_tag="VNP73_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HWL65559.1"
                     /translation="MSTRMPLSIALVVLMASLTAGSPTASAAFPGENGLIAYRHEAEI
                     FTMTGDGSNKVAVPNTAASAAPTDPAWSADGNRLAFVRNQDIWIINASGTGARNLTNT
                     PTFLDAEPSWSPDGSEIAFGSYRDSNWEIYVVRSSDGGGERQLTQDTDPSRRDPSTFP
                     AWSPDGSQIAFFSDRPGAFGLYVMPADTGDPESFLVATSATNNYPSWFPDGSKLAYTN
                     DTELWVMDADGTDQTELTTGARVAFGVSVSPDGNSIAFTSGRDNNYEIYVADADGGNQ
                     VNITNSPGTDDRLPDWAVPGTDDGGVQNGTDEDDQLDGSGSSDTVYAGAGNDVIETLG
                     GADIAYGEEGNDTLNGGAGNDKLYGDFGPAPSAPNRSGRRFMQEEEPVTADDVIKGGG
                     GADVAVGGPGSDAINGGPGIDRMKGGPGIDTCVFSSKKEVAKSSSCERKKLNF"
     gene            3497..3913
                     /locus_tag="VNP73_06255"
     CDS             3497..3913
                     /locus_tag="VNP73_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWL65560.1"
                     /translation="MSSVELHKKAHELFNARDWDGLKGVLTDSLNYEDHPRGLSMKSG
                     DEFVAWAKEWTTAFSNARVGNPRYIDGGDWSICCFQAQGVNDGPMGDVPASGGTMDMP
                     FCEIFHWTNGKADRAEIYYDQVTMMTQLGVMEAQPA"
     gene            complement(3999..>5364)
                     /locus_tag="VNP73_06260"
     CDS             complement(3999..>5364)
                     /locus_tag="VNP73_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65561.1"
                     /translation="KNISSYDISDPTAPVLLAQLPVGFMFENEEVAIDPQGKFILFSE
                     SLPNDILHVYDVEDPTSITEVGGGVAGAGDHTTSCILECDYAYGSDGSITDLTDYKNP
                     KPIALDTDEDNWHNRVGLIGGGHHVREVRNGLIVSSNLDETPWVIDVKDPEKPNLVAV
                     GDGPKGWSGERGYLWHSAQWPNEANDRWLLMGGEDVLNPPKSSTCNEQQGPFSTFDTT
                     NWEKTHKVTQVDVHRVDNGVYADGNPPANAFGCTGHWFEEHESFNNGGLVTMAWYDHG
                     ARFLQITSAGKIKEVGHFLPYAGESSATYWVTDKIVYSADYSRGIDILQWNGPTFVPT
                     GGGGGGGTGGGDGGGGGGGSQLPGPGVKLRISDRNPDRGDTIGFRVALRRCKGHKGTT
                     VRLQRKLKGDFRTIASKPLRRNCLAKFTDVASYRRASYKAIWPKQDDDHRAGRSRPQS
                     VTPQ"
BASE COUNT          954 a         1694 c         1657 g         1059 t
ORIGIN      
        1 ttgctgcaca tacgctgcac gccgttgaat ctggtggaca gccgacaacc taaaaacacc
       61 ctctgacctg cgtgggcggt gacgggttcg aaccgccgac ccctgcgttg taagcgcagt
      121 gctctaccag gctgagctaa ccgccctccg gcctacggcc gtgatcctaa ggatacgggc
      181 agccctactc caccgttaga gccagaccgt ctatgcgcac cggtgcatcg tcggaaacca
      241 cgaccagcga cacatcaccc tcgcggatcg aggtgaacct cttgatgcgg atcaccgctg
      301 tgcgcgtgcg ccgagagcgg agatcgaccg tgccgatccg ccgcccgcgg agcatgacgg
      361 cgatccggcc gcacctccga cacgtctggg caacaacatg ggcctccctc acactcacgt
      421 tgcgggcatc gagtgaagct ccacgctctg tcgtcgtcgt gagcgtccgc aggaaggcac
      481 tccggttcga ggtggtgcgc cagctcgagg atcgtctcag atctcggtcg tcgaacggca
      541 cggtggtgca ccgaggatcg gaccacggag atctgttccc cgcctgatcc gtggcgcgag
      601 ccgagaagca ataagtagcg ccggtctcag ccctcagggt tgttctcgtc ctcgtcgtgt
      661 cgtcgaccaa gcgcttgtag tcccggaacc ttcctcctcg aaccgtcggc agataccgaa
      721 ccgcgtaatg cgcttgagac tcggaggctt cccattcgat cgtcagccgc gtatccaacg
      781 tctgctcgcg tcgcggcttg atcatgcgtg gctcttctgg atccgtcgag tccgtgacca
      841 caccgctcgg cgacgattcg ctggaggacg aaccggcact gttcgtggcg gtgacggtca
      901 ccttcatcgt gtgctggtag tcggcgggca ccagacggta ggtcggctcc gtcccgttca
      961 gtacgggaga gcagctcgat ccatcggcgt cgcaacgatg ccacgcgtac gagtacgcaa
     1021 gcggctcgga tccgctccac gaaccggacg tggcgttcag cgttcggccc acctccgtct
     1081 cgcccgatat ctccggaggc gattcgttcg ccggcggtgt cgcgtcgtcc ggcgtcggct
     1141 cgggctcggg agctcccggc accgtgtacg gagttagatc ggagccggcg aacttgtctc
     1201 cgtcgacgca acccgagata cccgggatcg tcgcgcaatt ggtccattgc cagaacgccc
     1261 acccgtggcc accccagttc gaagccggcg tgctcggcga cgacgcgctc gtgtaatgag
     1321 ccagccacag tgggaaacct tgatccgcga acgtcgtggt gtccgcgagg ctcgtcttcc
     1381 agaagttcgg ggacgtgtag atgagcggcc gtgcgtcgat ggcttcagcc actgcgtcga
     1441 cccatacctg cgtccacgcg atcaaccgat ccgacggcaa tcctccggtc gcttcgagat
     1501 cgaggaccgg caacagatcg ccggatgccg gctcggccac ctcgaggaag tgctcggcct
     1561 ccccttcggc gtcggcctcg gctgcctcga tcgtctcgcc gttgggacga gcgaagtgat
     1621 aagcgccgaa cgggacactc gcagccgatg cttcggcgcg attgcccgcg tactggtcgt
     1681 cgatgaaggt ggcaccctcg gtcgccttgt ggaacgcgaa tacgtgcccg ctgttggcaa
     1741 cctcgttcca gtcgatctcg ccctgatgat gtgagacgtc gatgccgcgc atgtactcct
     1801 gagcgtgcgc ggtgcccggc cccactacaa acgtggggat gactgcaaga accgcggtca
     1861 ttacgacggc tgtcttcatc cgccgagcct tacacggcta gaagttcagc ttcttcctct
     1921 cacacgagct cgacttggcg acctctttct tgctgctgaa gacgcaggtg tcgatgcccg
     1981 gccccccttt catccgatcg atgccgggac caccgttgat cgcgtcggac ccgggccctc
     2041 ccacggctac gtcggcgcct ccgcctccct tgatcacgtc atcggccgtg acgggttcct
     2101 cctcctgcat gaatcgtcgg cccgagcgat tcggggcact gggagcgggc ccgaagtcac
     2161 cgtagagctt gtcgttgccg gctccaccat taagcgtgtc gttaccttct tccccatacg
     2221 cgatgtcggc tccgccaagc gtctcgatga cgtcgtttcc ggctcccgcg tagacggtgt
     2281 ccgaggaccc cgaaccgtcc agttggtcat cctcatcggt tccgttctga acgccaccgt
     2341 catctgtccc cgggacggcc caatccggca aacgatcgtc ggtgccgggg ctgttggtga
     2401 tgttgacctg attgccgcca tcagcgtcgg ctacgtagat ctcgtagttg ttatctcgcc
     2461 ccgatgtgaa cgcgatgctg ttcccgtcgg gcgacacaga aactccgaag gccacacgtg
     2521 caccggtggt tagttccgtc tggtccgtgc cgtccgcgtc catcacccac aactccgtat
     2581 cgttcgtgta ggcaagcttg gacccgtccg ggaaccatga cggatagttg ttcgtcgccg
     2641 aggtcgcaac gaggaagctc tcagggtcgc ccgtgtccgc cggcatcaca tagagaccga
     2701 acgcaccggg gcggtcgctg aagaacgcta tctgagagcc gtccggcgac cacgcgggga
     2761 aggtgctggg gtcgcggcga ctgggatcgg tgtcctgggt caactgacgt tctccgccgc
     2821 cgtcactcga acgaaccaca tagatctccc agttgctatc ccgataactg ccgaacgcga
     2881 tctcggatcc gtccggggac cagctcggtt cggcatccag gaaagtcggc gtattcgtta
     2941 ggttccgtgc acctgttccc gaagcgttga tgatccatat gtcttggttc cgtacgaaag
     3001 cgagacggtt cccgtcggcc gaccaggcgg ggtcggttgg agcggccgag gcagccgtgt
     3061 tcggcaccgc gactttgttc gacccgtcgc cggtcatggt gaagatctca gcttcgtgcc
     3121 ggtaagcgat aagaccgttt tcgccgggga aagcggctga agcagtcggc gatcctgctg
     3181 tcaaggacgc catcagcaca acgagcgcga ttgacaaagg catgcgtgtg gacattgcag
     3241 agcccctctc tccagacgaa agctgaaaga gtcgagctct ggtaaagcgg ctcggccagc
     3301 ggtcgtccct gaatctggac gtttgcccgc ggtgtcgggt gcgtctcacg atacgcctcc
     3361 cattccagga gcgataggtc cttagtggag gactagtcct ccgaaatagt catctggcaa
     3421 ccatccatcg gaccggaccc cggcgaactt tgcgcgaagt ccagaaaagg gttgggctct
     3481 agcgagggag gtactcatgt cgagtgtcga gctacacaag aaggctcacg agctgttcaa
     3541 cgctcgggat tgggacgggt tgaagggggt cctgacggac agcctcaact acgaggatca
     3601 tccgcgcggc ctcagcatga agagcggcga cgagttcgtg gcgtgggcga aggagtggac
     3661 cacagccttc tccaacgcgc gcgtcggcaa cccgcgttac atcgacggtg gcgactggtc
     3721 gatctgttgc ttccaggctc agggtgtcaa cgacggaccg atgggcgacg tacccgcctc
     3781 cggaggaacg atggacatgc cgttctgcga gatctttcac tggacgaacg gaaaggccga
     3841 ccgcgccgag atctattacg accaggtcac gatgatgacg cagttgggag tgatggaagc
     3901 tcagccggct taggaattag aggaagctgc gagggctagt tgcccgctgt tcaaaggaac
     3961 gaagtcctct ttcaactagc cctcgcaaac ccgttctatt actgaggtgt aaccgactgc
     4021 ggccgtgacc tacccgcgcg gtggtcgtca tcctgcttgg gccatatcgc cttgtaactg
     4081 gccctgcggt aactcgcaac gtcggtgaac tttgctagac agttgcggcg gagcggcttg
     4141 gacgcgatcg tcctgaagtc gcccttcagc ttgcgctgga gtcgcacggt cgttcccttg
     4201 tgtcccttgc agcgtcggag tgccacacgg aatccgatcg tgtcgccgcg gtcggggttc
     4261 cggtcagaga tacggagctt cacgccgggg ccgggcagct gagaaccgcc gccgccacca
     4321 ccgccgtctc cgccaccggt gccaccgccg ccaccgccac cggtcgggac gaacgtgggc
     4381 ccgttccact ggaggatgtc gatgccgcgc gagtagtcgg ccgagtaaac gatcttgtcg
     4441 gtcacccagt aggtggccga ggactcaccg gcataaggca ggaagtggcc gacctctttg
     4501 atctttccag cactggtgat ctggaggaaa cgagcgccgt ggtcgtacca cgccatcgtg
     4561 accagtccac cgttgttaaa cgattcgtgc tcctcgaacc agtgccctgt gcaaccgaac
     4621 gcgttggcgg gtgggttccc gtccgcatac acgccgttat cgacgcggtg cacgtcgacc
     4681 tgggtcactt tgtgggtctt ctcccagttc gtcgtgtcga aggtcgagaa cggaccctgc
     4741 tgctcgttac acgtcgagct cttgggcggg ttgagcacgt cctcgccacc catcagaagc
     4801 cagcggtcgt tcgcctcgtt cggccactgc gccgagtgcc ataggtagcc gcgctcgccg
     4861 ctccagccct tgggtccgtc accgacggcc acgagattgg gcttctccgg gtctttcacg
     4921 tcgatgaccc acggtgtctc gtcgaggttc gaagaaacga tcagaccgtt gcggacctcg
     4981 cggacgtgat ggccaccgcc gatgagcccg acgcggttat gccagttgtc ctcatccgtg
     5041 tcgagagcga tgggctttgg attcttgtaa tcggttagat cggtgatcga gccgtcggaa
     5101 ccgtatgcgt agtcacactc gaggatgcac gaagtggtgt ggtcgccggc accggcaact
     5161 cccccaccaa cctccgtaat gctcgtggga tcctcgacgt cgtacacatg aagtatgtcg
     5221 ttgggaaggg actccgagaa caggatgaac ttgccctgcg ggtcgatggc gacctcttcg
     5281 ttctcgaaca tgaatccaac gggcagttgg gcgagaagga ccggcgctgt gggatccgag
     5341 atgtcgtacg aggagatgtt cttc
//
LOCUS       DATMED010000243         2880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4035109, whole genome shotgun sequence.
ACCESSION   DATMED010000243 DATMED010000000
VERSION     DATMED010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4035109"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VNP73_06265"
     CDS             complement(<1..762)
                     /locus_tag="VNP73_06265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWL65562.1"
                     /translation="MKGSVERVLAAVLILTQFVAIARATEGQPGRPMEVDAKELRYGT
                     ARQAGLLPEWIDRIDEEVEAGLQPSPEKPLYPGAVVIAGRNGVIAKHEAFGYAYKYSD
                     GAPTLLPEADRVPMGKETIFDLASVSKLFTSIAVMQLAEEGNIDLGETVSSYIPAFGR
                     NGKDSITVKMLLTHTSGLAAWLPLYRDYPSPPERIEAIYNSAPIYPPGTDYVYSDLGM
                     IVLGELVETVSGEPLDGYVATHITEPLGMTDTGYNP"
     gene            complement(779..2074)
                     /locus_tag="VNP73_06270"
     CDS             complement(779..2074)
                     /locus_tag="VNP73_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HWL65563.1"
                     /translation="MLTRRQLLKSGGGALVGASLVGRAAPAVARSTGSRDRRVTIGVE
                     VLIKERLDDYAGRKFGIVTNPTGVLPDLAHEVDVLAAMRPEVGLEAVFGPEHGFRGTT
                     QAGGSEGFYIDEKTGLPVYDTYLKRGQELADIFTRSGVDVVMFDIQDAGSRFYTYIWT
                     LFDCMEAAVLSGKEFVVLDRPNPITGTAAEGPVLEPFYSTFVGRQPISQRHGMTVGEL
                     ARLFNGEFLPDTVGSSVELTVIEMEGWKRDTMYWDTGLPWVLPSPNMPTVDTALVYPG
                     TGMFEGTNLSEGRGTTRPFELIGAPYIKGALADSLRTKRLPGVQFREAYFIPTFSKYV
                     NETVGGFQTFVTDPHEFDPIHTTLTVIIETKRLYPEDFEWRYDSFDPQRPYWIDKLTG
                     SDHVRTSIDEGQSADEVVRGWKPELRVFQHTRRRYLLYR"
     gene            complement(2113..>2880)
                     /locus_tag="VNP73_06275"
     CDS             complement(2113..>2880)
                     /locus_tag="VNP73_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWL65564.1"
                     /translation="AIKAGADMMLMPPDLDLAYNAVLDAVESGEIGVGRINESVHRIL
                     SLKLEQGLFENPYVDVSKVSSSVGTPEHLAVADEITDKTITLVKNDEGVLPLEVDAGQ
                     DVLVTGWGVSSTQTLAAGIAERGATTEVYETGNNPTAVQRQTAATKAATKDLVVISSH
                     RAWASPEQQVLVDDLLATGTPVVVLAVRDPYDIAYFTDAPTYVATYSYSPVSLAAATR
                     ALFGEIDPAGKLPVAIPTAEDPNTELYPYGYGLSYED"
BASE COUNT          528 a          907 c          874 g          571 t
ORIGIN      
        1 ggggttgtag cccgtgtccg tcatgcccag cggttcggtg atgtgcgtgg ctacgtagcc
       61 atcgagcggt tcaccgctaa ctgtttcgac gagctcgccc aacacgatca ttcccaagtc
      121 gctgtagacg tagtcggtac ccggcggata gatcggggcc gagttgtaga tggcctcgat
      181 acgctccggc gggctcgggt agtcccggta caaaggcagc catgcggcca ggcccgaggt
      241 atgcgtcagc aacatcttga cagtgatcga atcctttccg tttcggccga aggccgggat
      301 gtaggaggac accgtctccc cgagatcgat gttgccctcc tccgccagtt gcatcacggc
      361 aatcgaagtg aagagcttgg agacggacgc gaggtcgaag atcgtctcct tgcccatcgg
      421 cactcgatcg gcttcgggca gcagagtggg tgcgccgtcg gagtacttgt atgcgtaccc
      481 aaaggcctcg tgcttggcaa tgacgccgtt cctgccggcg atcaccaccg ccccggggta
      541 gagcggtttc tcgggcgagg gctgcagacc cgcctccacc tcctcgtcga tgcgatcgat
      601 ccattcgggg aggagtccgg cctggcgcgc ggtcccgtag cggagttctt tcgcgtcgac
      661 ttccattggt cgacccggtt gaccctccgt tgcccttgcg atcgcaacga attgcgtgag
      721 gatcagcacc gctgcgagaa cgcgctctac agagcccttc atggttccct cctatgacct
      781 agcgatagag caggtagcga cgccgcgtgt gctggaacac acgcagctcc ggtttccaac
      841 cgcgtacgac ctcgtcggcc gactgtcctt cgtcgatcga cgtgcgcacg tgatccgagc
      901 ccgtcaactt gtcgatccag taaggccgct ggggatcgaa cgagtcgtag cgccactcga
      961 agtcctctgg gtaaagtcgc tttgtttcga tgatgaccgt gagcgtcgtg tggatagggt
     1021 cgaattcgtg cggatccgtt acgaaggttt ggaaaccacc gacggtctcg tttacgtatt
     1081 tgctgaaggt ggggatgaag taggcctcgc ggaactgcac gcccggcagg cgcttggttc
     1141 gaaggctgtc ggcgagagca cccttgatgt agggagcccc gatcaactcg aaggggcggg
     1201 tggtcccacg tccctccgag aggttcgtcc cttcgaacat tccggttccc gggtagacga
     1261 gcgcggtgtc gaccgtaggc atgttgggcg aaggaagaac ccagggaagc ccggtgtccc
     1321 agtacatggt gtcgcgcttc cacccctcca tctcgatgac ggtcagctcg acagatgacc
     1381 ccacggtgtc gggcaggaac tcgccattga acaaccgggc aagctcaccg acagtcatgc
     1441 cgtggcgctg ggatatgggc tgacggccca cgaacgtcga gtagaacggt tcgagcacag
     1501 gcccctcagc cgcggttccc gtgatgggat tgggccgatc gagcaccacg aactccttgc
     1561 cggagagaac ggccgcttcc atgcaatcga acagggtcca gatgtaagtg tagaaacgag
     1621 accccgcgtc ctggatatcg aacatcacga catccacacc cgagcgcgtg aagatatcgg
     1681 caagctcctg tccccgtttc aggtaggtgt cgtacacggg aagtccggtc ttctcgtcta
     1741 tatagaagcc ctcggagcca cctgcctgcg tggtccctcg gaatccgtgc tcggggccga
     1801 agaccgcttc cagtccaacc tcggggcgca tcgctgcgag cacgtcgacc tcatgagcca
     1861 ggtcgggaag caccccggtc ggattcgtaa cgatgccgaa cttgcgaccg gcgtaatcat
     1921 ccaggcgctc cttgattagc acctccacac cgatcgtgac gcggcgatcg cggctaccag
     1981 tcgagcgcgc cactgcgggc gcagctcgtc ccaccagcga tgctccgacc agcgcgccac
     2041 cgccgctctt gagcaactgc cgtcgagtaa gcactcggct cccctcctct tgctcctgtc
     2101 tttgtctgcc gatcagtcct cgtacgacag gccgtatcca tacggataga gctcggtatt
     2161 cgggtcctct gcagtcggga tcgctaccgg aagcttcccg gccggatcga tctcgccgaa
     2221 gagcgccctc gtcgctgccg cgagcgatac agggctgtag ctataagtcg cgacgtaggt
     2281 cggggcgtcc gtgaagtagg cgatgtcata cgggtcacga accgcgagca cgaccacggg
     2341 agttcccgtt gcaagaagat cgtcgaccag aacctgctgt tcgggacttg cccacgccct
     2401 atgggaagag atcaccacca ggtccttagt ggcggccttg gttgccgccg tttgtcgctg
     2461 gaccgctgtt gggttgttgc cggtctcgta cacctcggta gtcgcgccgc gctctgcgat
     2521 gccggccgcc agcgtctgcg tcgaggacac gccccaaccg gtcacaagca cgtcctgacc
     2581 cgcgtccacc tcgagcggga ggacaccctc gtcgttcttg accagggtga tggtcttgtc
     2641 ggtgatctcg tcggcaactg caaggtgctc tggcgtcccg acacttgacg acaccttgct
     2701 cacatctaca taggggttct cgaacaaacc ctgctccagc ttcaagctaa gaatccgatg
     2761 caccgactcg ttgatgcggc cgacaccgat ctctccactt tccacggcat cgagcaccgc
     2821 gttgtacgcg agatcgagat ccggtggcat gagcatcatg tccgcgcctg ccttgatcgc
//
LOCUS       DATMED010000244         5050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_944311, whole genome shotgun sequence.
ACCESSION   DATMED010000244 DATMED010000000
VERSION     DATMED010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944311"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VNP73_06280"
     CDS             <1..392
                     /locus_tag="VNP73_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWL65565.1"
                     /translation="VLIHGYDITKAEGRPWHIDPHAAALSGRGVSPMAIDYVDKEAAA
                     GLRACFDLRMRGQWQLHYVFSDGELSIEEPTDRRVDVHISADPVAFLLVAYGRISQWG
                     PIATGKIAAWGRKPWLAMRFGSLLRNP"
     gene            528..1364
                     /locus_tag="VNP73_06285"
     CDS             528..1364
                     /locus_tag="VNP73_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HWL65566.1"
                     /translation="MRRASALILLAALLMAPACGGGEGDSSAAIGLNDPIGAPEEDPT
                     SAPSLAEDPSVIKTASVQMDVSRPDLNAAAQSVVDLATTDAVGGFLVSSVLDTNDGFG
                     MAQVVVKVPSPVFEDVVSGLEDVGNITRQELEGEDLSGSALAVHARLRETRARFGSLM
                     RRIQRSDDGIVRAELRTALRDVRGNLTRLENDESHIQRVTSFSTIDVAIVGKQPPAPP
                     QKPALERSIETAMNIMVSIASGVILAAGVVVPLALIAVVIYLIWRSFVRRLRLPPATS
                     RP"
     gene            1409..2392
                     /locus_tag="VNP73_06290"
     CDS             1409..2392
                     /locus_tag="VNP73_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWL65567.1"
                     /translation="MTEWVIPTLDDVHAAQERIAPHLPRTPMYHYPALDELTRAETWV
                     KHENHLPSGAFKIRGGINLMSKMSPEERRTPVIAASTGNHGQSIAYAAQLFGLKAIIC
                     VPEDANAVKLAAMERSGAEIVMHGVDFDDAREHCEKLAEANGYRYIHSGDEPDLIAGV
                     GTYTVEILDEVPDIEVIIVPIGGGSGAAGTAIVAKATRPEIRVIGVQSAAAPAAWRSW
                     KGRELVEDKMGTAAEGLATRTAFELPQRILWDLLDDFVLVEEEQLQAADFHMIERTRN
                     LVELAGAAPLAAAMSTEVKPQIAGKKIALICSGANITPQQLRSVLERNAGQ"
     gene            complement(2411..2953)
                     /locus_tag="VNP73_06295"
     CDS             complement(2411..2953)
                     /locus_tag="VNP73_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65568.1"
                     /translation="MRPFRMLVALMVAAMMLFVACGGGGSTPAGAPVEQDELEDAAED
                     AAEDAVEGSEDVADGILANRECRFAIDALRNNPVAGAFTGDTDPKALDESVEFFRDFA
                     SKAPDEIREDLTYMSEYYARVVETFIKSDFNPETAASNPEAAQKLAAEFEKIEKEFPA
                     KQVEAASKNTSDWFAENCEK"
     gene            complement(3110..3292)
                     /locus_tag="VNP73_06300"
     CDS             complement(3110..3292)
                     /locus_tag="VNP73_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65569.1"
                     /translation="MKRRVALMVVGLLAAGSLQVTVAPAASASETCADPHCPWSPVTY
                     PVRLLCRDALPELCPL"
     gene            complement(3376..4638)
                     /locus_tag="VNP73_06305"
     CDS             complement(3376..4638)
                     /locus_tag="VNP73_06305"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL65570.1"
                     /translation="MDHEGRTPIVHQFRTGLAAAEPTIGFTRHGDLFFSAMESNTRIE
                     VLRSGDKGRQWDIVSPRLDDETNAHLLSFDPYLYVDERTSRVFNVDLTVACSYLSYSD
                     DRGESWATNPLACGRPINDHQTLFAGRPVNSNTVDYSNVVYYCWNDVGTSSCSKSLDG
                     GESFAPTGAPAFVGVNPSGEGQTGPFCGGVTGHGSADTRGTIYLPKAHCEQPWLAISD
                     DEGTTWSQVQVADIGTYRYGSDPSVAVDKRGTIYYLWIAHDRLPYLTVSSDRGQSWSR
                     PMMVGAPGLTEADLPSLDVGKPGHIALAYYGSRNSPFRGCEGSPTCEPDLDYSDAAWS
                     GYVSVSTDALSPNPLFLTGTVNDRRDPLVRGACGPGRCSAVYDFIDVEIAPDGTAWAA
                     YVDAWIGPHSSRIPAEGIVGRVVGTSLR"
     gene            complement(4788..>5050)
                     /locus_tag="VNP73_06310"
     CDS             complement(4788..>5050)
                     /locus_tag="VNP73_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65571.1"
                     /translation="CVRFKPRSGERFVSVEIEDGLGQPVYFWIGQREEDLQEYCGTMD
                     KPLRIQPRLEITVFLIEGTCRGTYTPSVVSRGTVTATFTNRR"
BASE COUNT          987 a         1579 c         1553 g          931 t
ORIGIN      
        1 aggtactgat ccacggctac gacatcacta aagccgaagg tcgcccgtgg cacatcgacc
       61 cccacgccgc cgcgctgtcc ggtcgcggtg tctcacccat ggccattgac tacgtggaca
      121 aagaggcggc cgccggatta cgcgcttgct tcgacctgcg catgcggggc cagtggcaac
      181 tgcactacgt gttctccgac ggagagttgt cgatcgagga gccgaccgac cggcgcgtcg
      241 atgttcacat ctccgccgac ccggtggcgt tccttctggt ggcttatggg cgcatctcgc
      301 agtgggggcc gatagcgacg ggcaagatcg ccgcgtgggg ccgcaagccg tggctcgcga
      361 tgcgcttcgg ttcgctactc aggaacccct gagagaaagg ggggccgggc cccatgcgct
      421 tggagtgaga cttcccttgc tttagccaat cttctaacgg ttcccacctg aaaagacgag
      481 aacgcgcgat taggtagtgc gaaagcccgc tctaaaaggg ggaccccatg cgtagggcat
      541 ccgcactcat ccttctcgcc gcgttgttga tggcgcctgc ctgcggagga ggcgagggtg
      601 actcgtctgc ggcgatcggc ctgaacgacc cgatcggagc gccggaagag gatccgacat
      661 ccgccccctc gctggccgaa gatccgtcag tgatcaagac ggcctcggtg cagatggacg
      721 tctccagacc ggatctcaat gccgctgcgc agagcgtcgt cgacctggcg acgactgatg
      781 cggtcggtgg tttcctggtc tcctccgttc tggatacgaa cgacggattc ggtatggcgc
      841 aggtggtggt gaaagtgcct tcccccgtgt tcgaggatgt cgtgtccgga ctcgaggacg
      901 taggaaacat cacgcggcaa gaactagagg gcgaggacct cagtggcagt gccctcgccg
      961 tccatgcccg gctgcgcgag acccgtgctc gctttgggtc actgatgcgg cggatccagc
     1021 ggtctgatga cgggatcgtg agagccgagc tgcgcacggc gctgagggac gtgagaggga
     1081 acctgacgag gctcgagaac gacgagagtc acatccaaag agtcacctcg ttctcgacga
     1141 tcgatgtggc catcgtgggg aagcagccgc ctgcaccccc gcagaagccg gctctggaac
     1201 gatcgatcga gacggcgatg aacatcatgg tctcgatcgc ttcgggagtg atccttgccg
     1261 ccggcgtcgt ggttcccttg gcgctgatcg cggtcgtcat ctatctcatc tggagatcgt
     1321 tcgttcgtcg cctgagactc ccaccggcca cctcccgtcc gtgattgacg gagagatctc
     1381 ggtttttctg aacacctaga ctccacccat gaccgagtgg gtgatcccca cacttgacga
     1441 cgtccacgcc gcccaggagc gcatcgctcc ccatcttccg cgcacaccca tgtaccacta
     1501 tcccgcgctc gacgagctga ccagggcgga gacatgggtg aaacacgaga accacctccc
     1561 ctcgggcgcc ttcaagatcc ggggtggcat caacctgatg tccaagatga gtccggagga
     1621 gcgtcgaacg ccggtcattg ccgcctcgac cggtaatcac ggtcagtcga tagcgtatgc
     1681 agcgcagttg tttggactaa aggcgatcat ctgtgtgccg gaggacgcca acgccgtgaa
     1741 gctcgcggcg atggaacgga gcggcgccga gatcgtcatg cacggtgttg atttcgacga
     1801 cgctcgggaa cactgcgaga agctcgccga ggccaacggt taccgctaca tccattcggg
     1861 agacgaaccc gacctgatcg ccggcgtcgg gacctacacc gtcgagatct tggacgaggt
     1921 gccggatatc gaggtgatca tcgtgccgat aggtggcgga agcggagctg cggggaccgc
     1981 gatcgtggcg aaggcaacac ggccggagat ccgggtgatc ggcgtgcagt cggcggcagc
     2041 gcccgcagca tggcgatcgt ggaaaggtcg ggagctcgtc gaggacaaga tgggcacagc
     2101 agccgagggc ttagcgacgc gaaccgcctt cgagcttccg caacggatct tgtgggacct
     2161 tctcgacgac ttcgtcctgg tcgaagaaga gcagctgcag gcggccgact tccacatgat
     2221 cgagaggacg cgcaacctgg tcgagctggc aggggccgct cccctggccg cggcgatgtc
     2281 gacggaggtt aagccacaga tcgccggcaa gaagatcgca ctcatctgca gcggggccaa
     2341 catcaccccg caacaactcc gatcggtgct ggaaaggaat gccggccagt agagagtctt
     2401 ccggctcgct ctacttttcg cagttctccg cgaaccagtc cgaggtgttc ttgctcgccg
     2461 cctccacctg tttcgccgga aattcctttt cgatcttctc gaactccgcg gccaacttct
     2521 gagcggcctc ggggttggac gcggccgtct caggattgaa atccgacttg atgaatgttt
     2581 ccaccactcg cgcgtagtac tcgctcatgt aagtgagatc ctcacggatc tcgtcgggag
     2641 ctttggaagc gaaatccctg aagaactcga cgctttcatc gagcgccttc ggatcggtat
     2701 ctccggtgaa cgcaccggcg acgggattgt tccgcagagc atcgatggcg aacctgcact
     2761 ctcggttcgc caggatgcca tcggcgacat cctcggatcc ttcaaccgcg tcctcggccg
     2821 cgtcttcggc tgcatcctcg agctcgtcct gctccacggg agccccggcc ggggtcgacc
     2881 cgccgccacc gcacgcgacg aacagcatca tcgcggccac catcagcgct accaacatcc
     2941 tgaaaggtct cacagctgct cctccttctt cgccgaagcc agcctgagtt agatcgcgcc
     3001 gggaccgcaa gtaactatcc gatttgtccc gtgagcggtt gttaacaagt ccggccccga
     3061 atctgcactc gatagaggag attcggggcc ggtgtgacgc gtgagccttt tagagcgggc
     3121 agagctccgg gagggcgtct ctgcagagaa gccttaccgg ataggtgacc ggcgaccagg
     3181 ggcagtgggg atctgcacag gtctcggaag cggatgccgc gggggcaacc gtgacctgca
     3241 gagaacctgc ggccagaaga ccgacaacca tcagtgcgac gcgacgcttc atagcgagct
     3301 ccttgatcgg aaggatgctg acgtcagaat cttcggctca ctcgaggcga actccttcac
     3361 ccaaaagagt gatgatcatc tgagcgaggt cccgacgacg cgaccaacta tcccctccgc
     3421 ggggatcctc gagctgtgag ggccgatcca tgcgtcaacg taggcggccc acgcggtccc
     3481 atcgggggcg atctccacat cgatgaagtc gtaaacggca gaacatcgac ccggcccaca
     3541 cgcacctcgc actagcggat cgcgccggtc gttcaccgtc cccgtcaaga aaagggggtt
     3601 tggagacaac gcatcggtag agaccgacac gtaaccgctc cacgctgcgt ccgaataatc
     3661 gagatcgggc tcgcacgtgg gcgaaccctc gcatcccctg aaaggtgaat tcctactccc
     3721 gtaatacgcg agcgcgatgt ggcccggttt gccgacatct agcgacggaa ggtccgcttc
     3781 ggtaagacct ggagccccaa ccatcatcgg ccggctccag ctttgaccgc gatccgaaga
     3841 aacagtcagg tacggaagcc gatcgtgggc gatccacagg taatagatcg tgccgcgctt
     3901 gtcgaccgct accgatggat cggagccgta gcgataggtg ccgatgtcgg cgacttgtac
     3961 ttgactccac gtcgttccct cgtcgtcgct tatcgccagc catggctgtt cgcaatgcgc
     4021 ctttggaagg tagatcgtgc cccgcgtgtc ggcactgccg tgtcccgtaa cgccgccgca
     4081 gaacggtccc gtctgaccct cccctgaggg gttcaccccc acgaacgccg gtgccccggt
     4141 aggcgcgaac gactccccac cgtcgagcga cttcgagcac gacgacgtcc ccacgtcgtt
     4201 ccagcagtag tagacgacat tggagtaatc gaccgtgttg ctgttgacgg gtcggccggc
     4261 gaacagtgtc tgatgatcgt tgatgggtcg cccgcaagcc aaaggattgg ttgcccaact
     4321 ctcccctcgg tcgtccgagt acgagaggta cgagcaagcc accgtgagat cgacgttgaa
     4381 gactcgcgac gtgcgctcgt cgacgtacag ataagggtcg aacgagagca agtgggcatt
     4441 ggtctcgtcg tctaatcggg gcgagacgat gtcccattga cgtcccttat ctccagaacg
     4501 gagcacttcg atacgcgtgt tgctttccat cgcgctgaag aacaggtcac cgtgacgcgt
     4561 gaatccgatc gtgggctccg ctgcggcaag tccggtccgg aattggtgca cgatcggcgt
     4621 gcggccctcg tgatccactc gatgaccgtc ggcgtgatgg gcgatcgcgg ctccccccga
     4681 tggcgccctt gagtcactag cgctcaccat gtgggccaac cacgcgacct gcgaccagag
     4741 aatgagaccg accagcggac ccagagctaa ggcctttgcg aggttcctca acggcggttc
     4801 gtgaaggttg ccgtcactgt tcctcgggat acgacgctgg gcgtatacgt ccccctacaa
     4861 gttccctcga tcagaaagac cgtgatctcg agacggggct ggatgcgcaa cggcttgtcc
     4921 atggtcccgc agtactcctg aagatcctct tcccgttgtc cgatccagaa atagaccggt
     4981 tgccccaatc cgtcttcgat ctcaaccgac acgaaacgct cgccggaccg gggcttgaat
     5041 cggacacaac
//
LOCUS       DATMED010000245         3075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5192712, whole genome shotgun sequence.
ACCESSION   DATMED010000245 DATMED010000000
VERSION     DATMED010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5192712"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1451)
                     /locus_tag="VNP73_06315"
     CDS             complement(75..1451)
                     /locus_tag="VNP73_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWL65572.1"
                     /translation="MRSARIYSWLVVLSTGGMLFWLYMDRGPAPELNRFVYWIAVLIA
                     IELLPVSLAFESQVVMSFPIHLAIAMLYPPWVSMTIAGIGAFDPREFKREVSLHQALF
                     NRAQQMLTVGIASAAFTPFREGLEQIDVGWNSVRGIVLAAVLATVVNLVLVALHVHYR
                     ASVPLSQAFGGLLPEPIAGFWLSYAVLTTLGGTTAIVYARLGEFGSWAIAAFLMPLLF
                     ARLTIMGARAKQQLSERVHQQQRALIEATEKIFTDREQERGRIAERIHDSSLQLLAGA
                     SYACQNAQELLDAGRQEEAERLLVSARSAIEEAIGSLRGSLVELRKPFVEQGGLLDAV
                     RDYLDQIETLWQVEVVFEADLKEEPPLSVSVGLFQILQEGLVNALKHAQTERITVKMK
                     ESAGRIHLIVQDEGAGFDPEAEVDEEHLGMQLMKRRAVELGGWLNLWSRPGEGTRLEA
                     VFPGAPDR"
     gene            1938..2966
                     /locus_tag="VNP73_06320"
     CDS             1938..2966
                     /locus_tag="VNP73_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWL65573.1"
                     /translation="MTKHQPVNLSVVIGDPRPRVVETLSRLIDNDGNADVVATSADPT
                     QTATLARQVRPDVLIIDGEEGFGDPIVSFSRTFLPETRVVVLTEDIRLEESGGLGVEA
                     QLAAISGDLWIMSLSDIDEAVRRNALPSALANAYVPAMRQKRHRDLATIEALAGAADL
                     RESKACGNLRRVTDLALSCLERVDNSLAASEEVRLGCVLHDVGKLGIPDAILHKPGKL
                     SSYEWLVMRRHPEIGVDLVEPIGLGSSAKEIILHHHEHWDGSGYPNGLAGQEIPIPAR
                     AFSVADAYDAMTSDRPYRRAMSRGDALQIIKSRAGRIFDEDMVDVLMSVVTIDLAESE
                     DVLIDLTG"
BASE COUNT          625 a          975 c          894 g          581 t
ORIGIN      
        1 cgcctccaaa ccctcggaga cgagcgtgtg atcctcggcg atcaaaacct tgacgctctt
       61 gacggtcttg tcgctcatcg gtcgggggcc cccgggaaca ctgcctcgag acgggtgcct
      121 tcaccgggcc gggaccacag attcagccag ccgcccagct cgaccgcgcg acgcttcatc
      181 agctgcatac ctaggtgctc ctcgtcgact tctgcttccg ggtcgaatcc ggcgccctca
      241 tcctgaacga tcagatggat gcggcctgcc gactccttca tcttgacagt tatccgctcc
      301 gtttgggcgt gtttcagcgc gttgaccaac ccttcctgaa gtatctggaa cagccccaca
      361 gagaccgata agggcggttc ctccttcagg tcggcctcga atacgacctc cacctgccat
      421 aacgtctcga tctgatcgag gtagtccctg acggcatcca gcagcccacc ctgctcgacg
      481 aagggcttcc gcagctcgac cagagaacct cgcagactcc cgatcgcttc ttcgatcgcg
      541 ctgcgggcgg acacgagcag gcgttcggcc tcctcctgcc ggccggcatc gagcagttct
      601 tgagcgttct gacacgcgta gctcgctccc gcgagaagct gaaggctgga gtcgtggata
      661 cgttccgcga tccggccccg ttcctgctcg cgatccgtga atatcttctc cgtcgcttcg
      721 atcagtgccc tttgttgctg atgaacgcgt tcggatagct gctgtttggc acgagccccc
      781 atgatcgtca gacgagcgaa cagcaaaggc atgaggaacg cggcgatcgc ccacgatccg
      841 aactcgccga gacgggcgta gacgatcgcc gtggtccctc caagcgtcgt cagcactgca
      901 tacgagagcc aaaacccggc tatcggctcg ggcaacagtc cgccgaatgc ttgcgatagc
      961 gggaccgacg ccctgtagtg gacgtgcaag gcgaccaaca ccagattgac gaccgtcgcc
     1021 aagaccgcgg ccagcacgat ccctcggacg gagttccatc ccacatcgat ctgctcgaga
     1081 ccctcgcgga agggcgtgaa cgcagccgac gcgatgccga cggtcagcat ctgttgggct
     1141 cggttgaaga gtgcttgatg cagactgacc tcgcgcttga actccctggg atcgaaggca
     1201 ccgatccccg cgatcgtcat cgagacccac ggcggataca gcatcgcgat cgcgagatgg
     1261 atcgggaagc tcatcacgac ctggctctcg aacgcgaggg acacggggag cagttcgatc
     1321 gcgatcagaa ccgctatcca gtagacgaac cggttcagct cgggggccgg cccgcgatcc
     1381 atgtacaacc aaaacaacat cccgcccgtg gacagaacga cgagccagct gtagatccgc
     1441 gcggacctca taccctcatg ctagttatga cctcgacaga aaaaaggtca ttagcgaagt
     1501 gaagtgtctc taaacgcgag ggaggagggc cgttggccct cctccaccaa gttctcaaga
     1561 cttcgtttag ctcttccagc cggcaccggc agtttggaac agtgccgtca ggatgcttac
     1621 gagtccgata agacggagct tcattttgag gccctcccgt gttgtcgagt ggctctccca
     1681 gttgcctgga aggttggacc tcgctccgct cctgtagcta cgtacttatg cctaccccgc
     1741 ttcgctagtg gtcggccttc tctccgaccg gaccgcgaat ttgacaccct gttaccccca
     1801 aaagcaatac gcgtgtccct cccgtggggg acgagttaac ccaaaggtgt accaaatcca
     1861 cctgcttcgg gggatagtcg tcaaccgact acggagcgtt aactacgtag agacgtggac
     1921 ggcgaaggga tctgctgatg acaaaacatc aaccggtcaa tctctcggta gtgatcggcg
     1981 acccccgtcc gcgcgtggtc gagacgctct cacgattgat cgacaacgac ggtaacgccg
     2041 atgttgttgc gacctcggcc gacccgacac aaaccgcaac tctcgcgcgg caggtaaggc
     2101 ccgacgtcct gatcatcgac ggcgaagagg gattcggaga tccgatcgtg tcgttcagcc
     2161 gcaccttctt gccggaaacg cgcgtcgtcg tgctcaccga agacatcagg ttggaagagt
     2221 ccgggggact cggcgtcgaa gcacaactcg cagcgatcag cggcgacctc tggatcatga
     2281 gtctgagcga tatcgatgaa gcagtacgta gaaacgctct cccgtcggca ctggcaaacg
     2341 cctatgtccc ggccatgcgg caaaaacgcc accgcgacct cgccaccatc gaagccctgg
     2401 ccggggcagc cgacttacgt gagtcgaaag cgtgcgggaa cctgcgacgc gtaaccgatc
     2461 tggcgctttc gtgtctcgag cgagtggaca actcgttggc ggcgagcgaa gaggttcgtc
     2521 tcggttgtgt ccttcacgac gttggaaagc tcggtatccc cgatgcgatc ctgcacaagc
     2581 ccggcaagct cagcagctac gagtggttgg tgatgcgccg ccaccccgag atcggtgtcg
     2641 atctggtcga gccgatcggc ctcggatcgt cggcgaaaga aatcatcctt caccatcacg
     2701 agcactggga cggcagcgga tacccgaacg gcctcgccgg ccaggagatc ccgatacccg
     2761 ctcgagcctt ctcggttgcc gatgcttacg acgcgatgac ctccgaccgg ccatatcgca
     2821 gagcgatgtc gcgcggcgat gccttgcaga tcatcaagag tcgggccggg cgcatcttcg
     2881 acgaggacat ggtcgacgtt ctgatgtcgg tggtgacgat cgatctggcc gagagcgaag
     2941 acgtcctgat agacctgacc ggctgaaaat ctagttgcaa ggcatctagc ccgaacgccg
     3001 acggcctcgg ctgtcggcgg gaactattcg tcttctgggt tggggcggcc taatacttcg
     3061 tttatggcga tcacg
//
LOCUS       DATMED010000246         3956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1342001, whole genome shotgun sequence.
ACCESSION   DATMED010000246 DATMED010000000
VERSION     DATMED010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342001"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..930)
                     /locus_tag="VNP73_06325"
     CDS             complement(196..930)
                     /locus_tag="VNP73_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWL65574.1"
                     /translation="MVTTEKRWKVGELATQTGLTVRTLHHYDEIGLLVPSERSPSGHR
                     LYGEQDVARLYRIVALRQIGLSLEEVKDTLSDSVDPVSTLKRHLEEIERRIELHEQLR
                     HRVTGLLATLERKGEPSVDHFIEALEVITRMEKYYTPEQLAQLEQRRNELGDDAIRNA
                     EQEWADLIAKAEEHMANGDDPASPEVQAIAKRWQELIEAFTGGDPGIRASLQKMYEDE
                     GPEKASRGMVNPELMAYIASAQGSGE"
     gene            complement(997..1374)
                     /locus_tag="VNP73_06330"
     CDS             complement(997..1374)
                     /locus_tag="VNP73_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL65575.1"
                     /translation="MITGVRSVGIYVSDQDRAKKFWMEHIGLQLIQDTPMGEGAGVPR
                     WIEVAPEDKSVVLVLFTPEGAESFVGTFSNVIFHCDDIQKTYEELTGKGVEFQDIPRK
                     EFWGWWATFKDSDGNTYGLGHKD"
     gene            1426..1887
                     /locus_tag="VNP73_06335"
     CDS             1426..1887
                     /locus_tag="VNP73_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWL65576.1"
                     /translation="MKSHTVYKTFNTDQRREFVRLTEDVQAAVDDSGVQEGMALVSAM
                     HITAAVWINDDEPGIQADALEWLDKIAPPSWKEPANEVADALSPDPGDYRHHRGGEDN
                     GDAHLKNLLVHHQVVVPVTDGKLDLGPWQAIFYCEFDGGRNKRLIIKVLGD"
     gene            complement(1958..2443)
                     /locus_tag="VNP73_06340"
     CDS             complement(1958..2443)
                     /locus_tag="VNP73_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65577.1"
                     /translation="MRRRLLATIAALGIVGALVATATPALAQAPKFHRADSSVADDAA
                     LVVTFDERGLGNEDIDYVLSADGEATYACFNRGGKNPSATNKRTVSQEVSQEATFEPR
                     NGRVQASIEAGPPSPGDFRCPSGQRMELIDVSYTNVVLTDTTNDVSTSVEDASRTFRD
                     A"
     gene            complement(2626..3306)
                     /gene="npdG"
                     /locus_tag="VNP73_06345"
     CDS             complement(2626..3306)
                     /gene="npdG"
                     /locus_tag="VNP73_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWL65578.1"
                     /translation="MTVDRVAVVGGTGDIGFALASRWAKAGIGVVIGSRDAGRAKEAA
                     DRLLKVVPGADVQGLENAEAVASVPTVLVAVPFSGLVPIYKSIAESLSADSIVIDATV
                     PVEASVGGKATHVFGVWEGSAAQLGLAFLPKGTKVCAAFHTLSASAVAELETPLEGDV
                     LVCGSKAGKEIVRGLVEVMPDLRFVDAGPLENARIIEPITALLIGINHRYKIDRAGIH
                     ITGLPKDF"
     gene            complement(3303..>3956)
                     /locus_tag="VNP73_06350"
     CDS             complement(3303..>3956)
                     /locus_tag="VNP73_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWL65579.1"
                     /translation="PENNIVRTALEALAAVLGGTQSLHTNGFDEALALPTEKAATIAL
                     RTQQVIGYESGVSDSSDPLGGSWLVESLTDEIEEKARDYIRKIDELGGAVQAIESGWM
                     KGEIEESAYRINQGIESGDRAVVGVNRFERGQEDPVEILSLDPELERRQVDGLKKIRK
                     ERDQAAVDAALKQLSEAAKGTDNLLYPMREALAAYATLGEVSDVLREEFGEYEPTAL"
BASE COUNT          676 a         1327 c         1171 g          782 t
ORIGIN      
        1 tgggatcctc atcgccagac gctccggatt gtggcgacgt tcgcagggga gcgttcttta
       61 tctcggtcgc cctcggcgtt gtgactttgg tgccggggtt cctcctcatc cttcaatggc
      121 tgatgcgccg tcgggaaatc attcccccgg aaggagcggc atgagtgcat gcacccatgc
      181 cgcgtccgcg acgacctatt cgccggatcc ttgggcgctg gcgatgtagg ccatcagctc
      241 cgggttgacc atcccgcgcg aggctttctc ggggccctcg tcctcgtaca tcttctgcag
      301 cgaggcgcgg atgccgggat cgccaccggt gaacgcttcg atcagctcct gccaccgctt
      361 cgcgattgct tgcacctccg ggctggccgg atcgtcgccg ttggccatgt gctcctcggc
      421 cttggcgatc agatcggccc actcctgctc ggcgttgcgg atcgcgtcat cgccgagttc
      481 gttccggcgt tgctcgagct gcgccagctg ctcgggcgtg tagtacttct ccatcctcgt
      541 tatcacctcc aatgcttcta tgaagtggtc gaccgacggc tcgcccttcc gctcgagcgt
      601 tgcgagcaag cccgtcaccc tgtgacgcag ctgctcgtgc agctcgatcc tgcgctcgat
      661 ctcttcgaga tggcgcttca gcgtcgacac cgggtcgacc gagtcgctca aggtgtcttt
      721 cacttcctcg agcgacaggc cgatctggcg caacgccacg atgcggtaga gcctcgcgac
      781 gtcttgctcc ccgtacagac ggtgtccgga ggggctccgc tccgacggga ccaacagccc
      841 gatctcgtcg tagtggtgaa gcgtcctcac ggtgagcccc gtctgcgtgg cgagctcgcc
      901 gaccttccaa cgtttctctg tggtgaccac cagaggacaa gctagagcct cacgtaacgt
      961 gaggtgcaac ccttcaaggc aagttttttc ggggcgctag tccttgtggc ccaggccgta
     1021 ggtgttgccg tcggagtcct tgaaagtcgc ccaccagccc cagaactcct tcctggggat
     1081 gtcctggaat tcgacccctt tgccggtcag ctcctcgtag gtcttctgga tgtcgtcgca
     1141 gtggaagatc acgttcgaga acgtgccgac gaaactctcc gccccctccg gcgtgaagag
     1201 cactagcacg acgctcttgt cctctggtgc aacctcgatc cagcggggaa ccccggcccc
     1261 ctcacccatc ggcgtgtcct ggatcagttg gagcccgatg tgctccatcc agaacttctt
     1321 ggcgcggtcc tgatcgctca cgtagatgcc gacactccgc acgcccgtaa tcattgcttc
     1381 ctcccgtctc gccgaagtac cgagggtagt ggaaggagcg gatcgatgaa gagccacacc
     1441 gtctacaaga cgttcaacac ggaccagcgc cgcgagttcg tgcgactcac ggaagacgtc
     1501 caggctgcgg tcgacgacag cggcgtgcaa gaaggcatgg cgctcgtaag cgcgatgcac
     1561 atcacggccg ctgtgtggat caacgacgac gagccgggga tccaggccga cgcgctggag
     1621 tggctcgaca agatcgcacc gccctcgtgg aaggagccgg cgaacgaagt cgcggacgcg
     1681 ctgtcacccg atcccggtga ctatcgtcat caccgtggcg gcgaagacaa cggcgatgcc
     1741 cacttgaaga acctcctcgt gcaccaccag gtagtggtcc cggtcaccga cgggaaactg
     1801 gacctcgggc catggcaggc gatcttctat tgcgagttcg acgggggccg caacaagagg
     1861 ctgatcatca aggtcttggg cgactaagaa ggggcgcctt tcggcgcccc ttctctaaac
     1921 cattcgaccg gagggtggat cgctatacgc gacctaccta agcgtcacgg aaggtgcgcg
     1981 acgcgtcctc aaccgatgtg ctcacatcgt tcgtcgtgtc cgttagtacg acgttcgtgt
     2041 aggacacatc gatcagttcc atccgttgcc ccgaaggaca cctgaagtca ccaggagacg
     2101 gcggaccggc ctcgatcgag gcctgaaccc ggccgttcct gggctcgaag gtcgcttctt
     2161 ggctaacttc ctggctcacg gtccgcttgt tggtcgcgga cgggttcttg ccaccgcggt
     2221 tgaagcacgc gtacgtggct tcaccgtcgg ccgacaggac gtaatcgatg tcctcgttcc
     2281 cgaggccccg ctcgtcgaaa gtaacgacca gcgctgcgtc gtccgccacg ctggaatccg
     2341 cgcgatggaa cttgggtgct tgagcgagcg cgggcgtggc cgtagccacg agtgccccaa
     2401 caatgcccaa tgctgcgatc gttgctagta accggcgtcg catgtgccct cctcttctag
     2461 aagtggcttg cgtgcacacc gcggcacgac aacagggaat gatggatgcc cctccggcaa
     2521 acttgtcggt cgcccgcgat ggaggagttt gcccctcacc cgtgcgttac ccggcttcga
     2581 ccggctaaac caattttctt ccaggacgga cagaaacgac gacggttaga agtctttcgg
     2641 taaccctgtg atgtggatgc cggcgcgatc gatcttgtag cggtggttga taccgataag
     2701 cagcgccgtg atcggttcga tgatgcgggc gttctcaaga gggccggcgt ccacgaaccg
     2761 aaggtccggc atcacctcca cgagtccccg cacgatctcc ttacctgctt tgctcccaca
     2821 tacgaggacg tcgccctcga gcggcgtctc taattccgcg acggcggatg cagacagcgt
     2881 gtggaacgcg gcacacacct tggttccttt gggaaggaac gcgaggccca gctgcgcggc
     2941 cgatccttcc cacaccccga agacgtgcgt tgcctttccc cccacggacg cttcgaccgg
     3001 gacggtcgcg tcgatgacga tcgagtcggc cgagaggctc tcggcgatgc tcttgtagat
     3061 cgggaccagt cccgagaacg gcacggcgac gagaaccgtc ggcacggatg cgacggcctc
     3121 ggcattctcg agcccctgaa cgtctgcccc gggaacgacc ttcagcaagc gatcggcggc
     3181 ttccttcgca cgacccgcgt cgcgcgagcc gatcacgaca ccgatgccgg ctttggccca
     3241 cctactagcg agagcgaacc cgatatcccc cgtacccccg actaccgcga cccggtcgac
     3301 cgtcataggg cggtgggctc gtactcgccg aactcttcac gcaagacgtc ggacacctcg
     3361 ccgagcgtcg cgtaggcggc caatgcctcc cgcatcgggt agaggaggtt gtccgttccc
     3421 ttcgcagctt ccgagagctg cttcaacgcg gcgtccaccg cggcctggtc ccgttccttc
     3481 cggatcttct tcaacccgtc cacctgtctg cgttcaagct cggggtcgag actcaagatc
     3541 tcgaccgggt cctcttgccc ccgctcgaag cgattcacac ccaccacggc gcggtctcca
     3601 gattcgatgc cctggttgat ccggtacgcc gactcctcga tctcaccctt catccagccg
     3661 gactcgatcg cctgcaccgc tccacccagc tcgtcgatct tcctgatgta gtcgcgcgcc
     3721 ttctcctcga tctcgtccgt cagggattcc accaaccacg agccacccag cggatcggaa
     3781 gagtccgaca ccccgctctc gtagccgatc acttgctgcg tcctgagggc gatcgtcgcg
     3841 gccttctcgg tcggcagggc gagcgcttcg tcgaacccgt tcgtgtgcaa cgattgagtt
     3901 cccccgagga cggcggcaag tgcctcgagc gcggttctaa cgatgttgtt ctccgg
//
LOCUS       DATMED010000247         6085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3270962, whole genome shotgun sequence.
ACCESSION   DATMED010000247 DATMED010000000
VERSION     DATMED010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270962"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VNP73_06355"
     CDS             <1..1472
                     /locus_tag="VNP73_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWL65580.1"
                     /translation="KGLRDLYESHHRVSYTDAALVAAATLADRYISDRFLPDKAIDLI
                     DEAGSRLRIRRMSAPPDYRELEEKIAEVRGQKESAIEAQDFEKAAGFRDDEKRLLDEK
                     AAKESEWKTAEGETMAEVNEEEIAEVLSSWTGIPVTSLTEEETAKLLRMEDELHKRIV
                     GQQDAIGAVSRSIRRTRAGLKDPKRPAGSFIFLGPSGVGKTETAKTLAEFLFGDEAAL
                     IQLDMSEYMEKHTVSRLVGSPPGYVGYEEGGQLTEAVRRRPYSVVLLDEIEKAHPDVF
                     NTLLQILEDGRLTDAQGKTVDFKNTVIIMTSNLGTQNLRKPAMGFGQASGELTYEKMK
                     ERVGEELKKGFRPEFLNRIDEVIVFHELSREEVKSIVDLMIRRVAEQLESQDVAIELT
                     EASKDHLARIGYDPALGARPLRRSIQRLIEDPLSEKILWKEFQAGETIVVDVEKDEKG
                     DDQIVFHKTQAAPDHPPVELAGAGVGDGEGETVPESGAS"
     gene            complement(1469..2119)
                     /locus_tag="VNP73_06360"
     CDS             complement(1469..2119)
                     /locus_tag="VNP73_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HWL65581.1"
                     /translation="MTSSPRQDDRPPLTRDRILDAALVLVDEYGMDGLSMRRLGAALG
                     VEAMSIYNHVASKHELLNGVVEKVLNEVEIPEPGGDWTQRIKEMARSYRKVAQAHPNV
                     MPLMAMRPFANLPTLPLVEEVFATLANANIAPEDSLAALRAVASYAVGFTTSEVAGFV
                     DDPAAYVANPSFDIESLSSFPHLLEMVPLMSDIDNDSHFEFGLDVCLQGIKAKLGV"
     gene            2351..3052
                     /locus_tag="VNP73_06365"
     CDS             2351..3052
                     /locus_tag="VNP73_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65582.1"
                     /translation="MVTPAHDGILLATDVHKVYRTGAESVVALGGIDVSIPRGEFLAV
                     MGPSGSGKTTFLNCLAALDDIDSGSVLIEGVDIHKLPDADRSHYRAQKMGFIFQSFNL
                     IPVFTAVENVELPLLLAGSDPSEARRRAESTLERVGLGHRKNHRPNELSGGEQQRTTI
                     ARALAGNPAIVWADEPTGNLDTETAGSIMELLSELNRDGLTLVLVTHDPAIGGLANRI
                     IRMRDGLIQSEDGGS"
     gene            3052..5913
                     /locus_tag="VNP73_06370"
     CDS             3052..5913
                     /locus_tag="VNP73_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWL65583.1"
                     /translation="MTPLVPFLVVFALIGYQALRRPVLRRLAFRDAVRRPTETMLVIA
                     GSLLGTALITGSLIVGDTLDASVRVTATTQLGPVDEIVQAPDEDTADRLVKRLNGLDE
                     ARIDGVTSLVTTQSAIASDASGRRLAEPSARLIELDFEDARTFGRDRAATGLRGGTPS
                     EGEIVIAEDLGEALEAGRGDRVTAYLYGRKLRFEVVRVLPQLGLAGFWKGFETTSMNA
                     FAAPGTLRSVARDAPKGTLPPEPIVLVSNRGGVEEGERLTTAVTGVIEDAFPPGASLR
                     VESVKRDRIERADREGEAFGEIFLAIGAFAIVAGVLLLVNIFVMLAEERKSQLGMLRA
                     IGMKRSHLVRLFVIEGAIYSFASAILGAVVGIGVGWAIVRLAAPIFSGFDAFSVDLLF
                     SVESSSIITGLSVGGLISVITVTGTSLRTARINIIRAIRELPEPRTFVARRRNLILGL
                     AAALLSGVWFAIGLGDQNGWPSAIAGPPLVAFSLTPVLARFVARRTALLAISAFALVW
                     GIFGNAILDDHFFEAGEIFAFVLQGVLLTFSAVIFLSQLQETLEGFLRRIAAKRLALR
                     LSIAYPLARRVRTGLTLGMFALVIFTMVFISTLSNVFGGQVDTATEKEGGFELLVDTN
                     ESNPPSEKELEAQRGVEQAVSLLSGAALWQPEGFTQPLYWPVSGIDRRFVAGGPPTLS
                     DRDESLSSDAEVWKRLLRDDSAVVIPQAFLQSGGGPPENLVEVGDTIALIDPLTGKRR
                     ERTVIGKVENDYAFSGVYFSKRALQSSVSQVTLSRFYIHAEGSPEELKDLAARIQGRF
                     VANGADAGSFRAIVEEFQQISLQFLKLMQAYLALGLLVGIAGLGVVMVRAVRERRREV
                     GVLRALGFLARQVRAAFVLESGFVALEGILVGVVLALITAAQLISSGDFGENIDFMIP
                     WGQLSLLTGTALVAAVMATAWPAQQASLIAPAEALRIAD"
BASE COUNT         1104 a         1836 c         1976 g         1169 t
ORIGIN      
        1 tgaagggtct ccgcgacctc tatgagtcac accaccgggt cagctatacg gacgcggccc
       61 tggtggcggc ggcgacgctg gccgaccggt acatctcgga ccggttcctg ccggacaagg
      121 cgatcgacct gatcgacgag gccggttcgc gcctgaggat ccgacgcatg agcgcgccac
      181 ccgactacag ggagctcgaa gagaagatcg ccgaggtccg agggcagaag gaatcagcta
      241 tcgaggctca ggacttcgag aaggccgccg gcttcaggga cgacgagaag cgtctgctcg
      301 acgagaaggc cgccaaggag tccgaatgga agacggccga gggcgagacc atggccgaag
      361 tgaacgagga ggagatcgcc gaggtccttt cttcgtggac cggcatcccc gtcacgtccc
      421 tgaccgagga agagaccgcc aagctgctcc gtatggaaga cgagcttcac aagcggatcg
      481 tcggtcagca ggacgcgatc ggcgcggttt ctcgctcgat cagacgcacg cgcgcgggac
      541 tcaaggatcc caagcgtccg gccggttcgt tcatcttcct cggtccttcc ggagtcggta
      601 agaccgaaac cgcgaagacg cttgcagagt tcctcttcgg agacgaggcc gcgctcatcc
      661 agctcgatat gtccgagtac atggagaagc acaccgtgtc gcgtctagtg ggttcgcccc
      721 cgggatacgt gggatacgaa gagggcggtc agctgaccga ggccgtgagg cgtcgtcctt
      781 actcggtcgt cctcttggac gagatcgaga aggcgcaccc cgatgtgttc aacaccctct
      841 tgcagatcct cgaagacgga cggttgaccg acgctcaagg caagacggtc gacttcaaga
      901 acacggtcat catcatgacc tccaacctcg gtacgcagaa tctgcggaag ccggcgatgg
      961 gcttcggcca ggccagcggc gagctgacct acgagaagat gaaggaacgc gtcggcgagg
     1021 aactgaagaa ggggttccgt cccgagttcc tgaaccgtat cgacgaggtc atcgtcttcc
     1081 acgagctgtc gagggaagag gtcaagtcga tcgtcgatct gatgatcaga cgtgtggccg
     1141 agcaactcga gagccaggac gtcgcgatcg agctcaccga ggcctccaag gatcacctgg
     1201 cgaggatcgg ttacgacccc gccctgggag ctcgaccact gcgtcgctcg atccagcgtc
     1261 tgatcgagga tccgctgtcc gagaagatcc tgtggaagga attccaagca ggcgagacga
     1321 tcgtggtcga cgtcgagaag gacgagaagg gcgacgatca gatcgtcttt cacaagacac
     1381 aggccgctcc cgatcacccg ccggtcgaac tggccggtgc gggtgtaggg gacggcgaag
     1441 gtgagacggt tccggagagc ggagcgagct agacgccgag tttcgccttg atgccttgta
     1501 ggcacacgtc gagaccgaac tcgaagtggc tgtcgttgtc gatatctgac atcagcggca
     1561 ccatctcgag taggtgcggg aacgagctca acgactcgat atcgaacgac ggattggcga
     1621 cgtaggccgc agggtcgtcg acgaaaccgg ctacctcgga ggtcgtgaac ccaaccgcgt
     1681 atgaagctac ggctcggagg gcggcaagcg aatcctctgg ggcgatattc gcgttcgcga
     1741 gggtggcgaa gacctcctcc accagaggca gggtcggcag gttcgcgaac gggcgcatcg
     1801 ccatcagcgg catcacgttg ggatgcgctt gagcgacctt ccggtacgaa cgagccatct
     1861 ctttgatcct ctgggtccaa tcgcccccgg gttcggggat ctcgacttcg ttcaggacct
     1921 tctccaccac cccgttgagg agctcgtgct tgcttgcgac gtggttgtag atggacatcg
     1981 cctcgacccc gagggccgcc cccagccgtc tcatgctgag cccgtccatg ccgtactcgt
     2041 cgacaagcac cagggccgcg tcgagtatcc ggtccctcgt cagaggaggg cggtcgtcct
     2101 gtctcggaga gctagtcatt tcgaatcatt tgtccttcgc cgccgccgaa agagagtctt
     2161 tctcttgact ccccaccctg ctccgcggta ctccttacac cgtaagtgtt gcgcccccgc
     2221 tccctgcccc ccggggagcg gggtagcgcc cacttacttt tttttgtggg cgtccctatc
     2281 ctcgggcgga ttttgcggat cgatccggat ggatcggatc tctacacact gggttaagaa
     2341 gcttgcctaa atggttactc cggcgcacga cggaatcctt ctagctaccg acgtccacaa
     2401 ggtttatcgg acgggggccg agagcgtcgt tgcgctgggc gggatcgacg tttctatccc
     2461 gcgcggcgaa ttccttgcgg tcatgggccc gtccgggtcg gggaagacga cgtttctcaa
     2521 ttgcctggct gcgctggacg acatcgactc cggcagcgtc ctcatcgaag gggtcgacat
     2581 ccacaagctg ccggacgcgg atcgcagcca ctaccgggcc cagaagatgg gcttcatctt
     2641 ccagtctttc aacctcatcc cggtgttcac cgcggtggag aacgtcgagc tgcctctcct
     2701 tttagccggg tccgatccct cggaggcgcg tagacgggcc gagtcgacgc tcgagcgagt
     2761 ggggctgggc caccgtaaga atcaccggcc gaacgagctt tcgggtggcg agcaacaacg
     2821 caccacaatc gctcgagcgt tggccgggaa tcccgcgatc gtgtgggccg acgaacccac
     2881 cgggaacctc gacacggaaa cggcaggctc gatcatggag cttctgtccg aactgaaccg
     2941 agacggattg acgctggtgc tcgtaactca cgatccggcg atcggtgggt tggccaaccg
     3001 gatcatccgg atgcgcgacg gcctcattca gtcggaagat ggcggctctt gatgacgccc
     3061 ctcgttcctt tcctggttgt cttcgcgctc atcggttatc aggccctccg acggccggtc
     3121 cttcgccggc tcgcgttccg ggatgccgtg cggaggccga ccgaaacgat gctggtgatc
     3181 gcgggaagcc tcctaggaac tgcgttgata accggaagtc tcatagtcgg agacacgttg
     3241 gatgcctcgg ttcgcgttac ggcgaccaca caactcggac ccgtcgacga gatcgtgcag
     3301 gcaccggacg aggacacggc cgaccgtttg gtgaagcgcc tgaacgggct ggacgaagcc
     3361 cgcatcgatg gggtcacttc gctcgttaca actcaaagtg cgatcgcatc cgatgcttcg
     3421 gggaggcgat tggccgaacc gtcggcgcgt ctgatcgagc tggacttcga ggacgcccga
     3481 accttcgggc gcgaccgagc ggcgacagga ctacggggcg gcactccatc cgagggggag
     3541 atcgtcatcg cggaggacct tggcgaggca ttggaggccg gccggggcga tcgcgtgacc
     3601 gcctatctct atggacggaa gcttcgattc gaagtcgtgc gggtgttgcc tcagctcggg
     3661 ctggcggggt tctggaaggg tttcgagacc acctcgatga acgcgttcgc ggcccccggc
     3721 acgctccgat ccgtagcgcg cgacgcaccg aaagggacgc tccctccgga accgatcgtg
     3781 ctcgtatcga atcgcggcgg ggtcgaggaa ggtgagcgac tcacgaccgc cgtgaccggt
     3841 gttatcgagg atgcgttccc gccgggggcc agcttgaggg tcgaatccgt gaagagggac
     3901 cgtatcgaga gggcggatcg cgaaggggaa gcgttcggcg agatcttcct tgccatcggg
     3961 gccttcgcca tcgttgccgg cgttctgctg ctcgtgaata tcttcgtgat gctggcagag
     4021 gaacggaaga gtcagcttgg gatgctgcgc gcgatcggga tgaaacgctc gcatctggtg
     4081 aggttgttcg ttatcgaagg tgcgatctac tcgttcgcat cggcgatcct gggcgcggtc
     4141 gtcggcatag gggtcggctg ggcgatcgtc cggctggccg caccgatctt ctctggattc
     4201 gacgcgttct ccgtcgatct tctcttctcg gtcgagtcca gcagcatcat caccggtttg
     4261 agtgtcggcg gactgatatc ggtcatcaca gtgaccggta cgagtttgcg aacggcccgc
     4321 ataaacatca tccgagcgat ccgagagctg cccgaacccc gcacgttcgt cgcgcgacga
     4381 aggaatctga ttctgggcct ggcagccgcc cttctctccg gcgtgtggtt cgcgatcggt
     4441 ctcggagatc agaacgggtg gccctcggcg atagccgggc cgccgctggt cgctttctcc
     4501 ctaactcccg tactggcgcg tttcgtggct cgtcggacgg ctctcctcgc gatctctgct
     4561 tttgccttgg tgtgggggat cttcggcaac gcgattctcg acgaccactt cttcgaagcc
     4621 ggcgagatct tcgcgttcgt gttgcaggga gtgttgctga ctttctcggc agtgatcttc
     4681 ctgagtcaac tacaggagac gttggaggga ttcttgagac gcatcgcagc caagcgtttg
     4741 gccctccggc tcagcatcgc ctatccgctg gctcgtcggg tcagaaccgg tctgacgctg
     4801 gggatgttcg cgctcgtcat cttcacgatg gtcttcatct ctaccctctc gaacgtcttc
     4861 ggcggtcagg tcgacaccgc gaccgagaag gaaggtgggt tcgagttgct cgtggacacg
     4921 aacgagtcca accctccatc tgagaaagag ctggaagccc aacgcggcgt cgagcaagcc
     4981 gtgagcctgc tgtcgggtgc cgcgctgtgg cagcccgagg gtttcaccca acctctttac
     5041 tggccggtgt ccggcatcga tcgccgcttc gtcgccggtg gccctccgac actctccgat
     5101 cgagacgagt ccctctcatc cgacgccgag gtctggaaac gactcttgcg tgacgacagc
     5161 gccgttgtca tcccccaggc gttcctgcag agcggtggag gtccaccgga gaacctcgtc
     5221 gaggtgggcg acaccatcgc ccttatcgat ccgctgacgg gaaagagacg agagcggacg
     5281 gtcatcggca aggtagagaa cgactatgcg ttctccgggg tctatttctc gaagcgggcg
     5341 ctgcagagct ccgtcagcca agtcaccctg tcgcgcttct acatccacgc cgagggctcg
     5401 ccggaggagt tgaaggacct tgcagcccgg atccaaggac gcttcgtcgc gaacggagcc
     5461 gacgccggga gcttccgtgc gatcgtcgag gaattccagc agatcagcct tcaatttctg
     5521 aagctgatgc aggcctatct agcgttggga cttctcgtcg ggatcgcggg attgggggtc
     5581 gtgatggtgc gcgcggtgcg cgaacgcaga cgcgaggtcg gcgtcctgcg ggctctggga
     5641 ttccttgccc ggcaggtgcg ggcggcgttc gtgctcgagt ccggtttcgt ggctctcgaa
     5701 ggcatcctcg tcggggtggt gctggctttg atcacggccg ctcagttgat ctcgtcgggg
     5761 gatttcggag agaacatcga cttcatgatc ccgtggggcc agctctctct cctgacgggg
     5821 accgcgttgg tggctgccgt gatggcgacc gcgtggcctg ctcagcaggc ttccctcata
     5881 gccccggcgg aggctttgcg gatcgcggac tagaacgctc atcgacccac cctgaaaatt
     5941 gtcgcaccct gtctctacgg tgacccgatg agcaagaaca agaaagaagc cggcttcctt
     6001 tgcagccgtt gcggccacga ttcaccgcgc tggttcggcc gctgcggcga ctgcggcgaa
     6061 tggaacagcg cggcccccaa ccggg
//
LOCUS       DATMED010000248         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3641112, whole genome shotgun sequence.
ACCESSION   DATMED010000248 DATMED010000000
VERSION     DATMED010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641112"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VNP73_06375"
     CDS             complement(<1..382)
                     /locus_tag="VNP73_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWL65584.1"
                     /translation="MFVAALVCVTSPAPVEAAEGNPLQLVAENRISERRLDLTFTTPA
                     LANPTTVRILLPEGFDPAAATRYPVLYLLHGGAADYTSWTTGGSAHLLTEDLPLITVM
                     PDAGRSAWYTDWYNNGLGGPPMWET"
     gene            complement(547..621)
                     /locus_tag="VNP73_06380"
     tRNA            complement(547..621)
                     /locus_tag="VNP73_06380"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(586..588),aa:Cys,seq:gca)
     gene            682..1299
                     /locus_tag="VNP73_06385"
     CDS             682..1299
                     /locus_tag="VNP73_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HWL65585.1"
                     /translation="MTALAHSIGRRQTQTAALTSDAVLVMGGLIFMWLLTQVAIPLEP
                     FSPVPITGQTLAVLVIGAAFGAVRAGITIGLYLALGAANLPRAFAEGNTGIEFLKAAS
                     PTGGYLVGFLIAAVVVGYLAQRGWDRSVGSALGAMFIGEVIVFTLGVAWLANALDVGA
                     QKAMEFGLYPFIIGDVIKLALAAGLLPTAWRFIKDKASGPTFKRS"
     gene            complement(1286..1741)
                     /locus_tag="VNP73_06390"
     CDS             complement(1286..1741)
                     /locus_tag="VNP73_06390"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HWL65586.1"
                     /translation="MPESKQDRELIELLNELRVALPGVQVMFAFLLMVPFSQGWALTT
                     ATQKGVYYAAFVFSTLAILMLISPTTYHRIRFRDRDKEHLIKLSNRLAIAGTVFLATA
                     MSCVVYFVTDYTFPNITAVATGILAGLFAWFWFGLPILREIREPSAKTS"
     gene            complement(1743..2771)
                     /locus_tag="VNP73_06395"
     CDS             complement(1743..2771)
                     /locus_tag="VNP73_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWL65587.1"
                     /translation="MLTNAQLAELLARAAEENEGHRQRALRSASRAAYHWPEEVADIV
                     ERGDSLRDLPKIGGWLSGIIRRWLEDPPEVPDPPPIRAGFRTFAEALAIVADNPEWSS
                     GLRGDLQMHTEYSDGKESIVAMAGEGAERGYEYIAITDHSKGLRIAGGMHEEELLAQI
                     DEIDVVNEELRKAGHDIRVLRALEMNINPQGEGDMEPESLEPLDLVLGSFHSSLRTKD
                     DQTDRYLAAVRNPHFQILGHPRGRKYDNRLGLQADWPRVFEEATKHDKAIEINSFPDR
                     QDLNSELLEIAREAGVRVSIGTDAHDRMEMRFIPMGVAAAIKAGIDRERVINYMSVDD
                     LVAWAKGH"
     gene            2808..3848
                     /locus_tag="VNP73_06400"
     CDS             2808..3848
                     /locus_tag="VNP73_06400"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWL65588.1"
                     /translation="MPLPFDPPIAPMLAKLQPEIPIGEDWRYEPKWDGFRAIVFRDGS
                     EIHIVSRDERPFERYFPELIPLFKEFLPKTCVVDGEIVIPGTEKLAFDALLLRIHPAA
                     SRVKMLSETTPASFVAFDLLAHGSKDLRETPLAKRRTLLEKSLGGYAIPGDATKRKVM
                     SALRPAPRIALTPHTDDPKLAKRWFEVFEGAGLDGVIAKKEDLHYRPGDRAMVKIKHR
                     RTADCVVGGYRLSKTGDGIGSLLLGLYDETGSLHYIGHTSAFKADERKRLLKDLKKLE
                     GGSSFGGGRVPGGPSRWTGGRDVSWVPIEPILVLEVSYDYLQGNRFRHAAGFVRWRDD
                     KKPKECTLDQVL"
     gene            complement(3900..>4468)
                     /locus_tag="VNP73_06405"
     CDS             complement(3900..>4468)
                     /locus_tag="VNP73_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWL65589.1"
                     /translation="LNEALKQTKKLREEGMEVPVAVNLSARSLLDEDFPDHVEGALRR
                     LQVPPSSLQFELTESTLVSDPKRALGVLSRLSQMGVKLSIDDFGTGYSSLSYLRRMPL
                     AELKIDKSFVLNMTVDESDLVIVQSTIDLGRNLGLQVVAEGVETADVWKKLQELGCDY
                     AQGFFHSRPMPGEELMQWLSVMSKGWGL"
BASE COUNT          835 a         1420 c         1388 g          825 t
ORIGIN      
        1 acgtctccca catcggcggc ccacccagtc cgttgttgta ccaatccgtg taccaggcac
       61 tccggccggc atccggcatc accgtgatga gtgggagatc ctccgtcagc aagtgcgcgc
      121 tcccgcccgt ggtccacgac gtgtagtccg ctgcgcctcc gtggagaagg tagaggaccg
      181 gataacgcgt cgcggcagcg ggatcgaaac cttccggaag cagaatcctt accgtcgtcg
      241 gattggcgag agcgggagtc gtgaaggtga gatcgagcct gcgttcggag atgcggttct
      301 ccgccacgag ctgtagcggg ttcccttcgg ccgcctcgac gggtgcaggg gaggtgacgc
      361 agacgagagc cgccacgaac attgcggcaa acaacccact ccagaggcgc agagacgagc
      421 cgggagatcg catagctgag gcttcgacac cagcccaggc aaccctgcct tggctggtgt
      481 cgacgggctc ggcccggtcg tggcccgcac gaacgccggc gggctcggcc gtcggcgaaa
      541 agagattgga ggcggcgagc ggaatcgaac cgctgtaaag ggttttgcag acccttgcct
      601 gaaccactcg gccacgccgc ccaggctggg agaggatagc cgggtataaa tcgtgcctat
      661 ctatctctca gggaggaact catgacggca ctcgcacatt ccataggcag gcgacagact
      721 caaaccgcgg ccctcacctc ggatgccgtg ctggttatgg gcggcctcat cttcatgtgg
      781 ctcctcaccc aggtcgccat cccgctcgag ccgttctcgc ccgttcccat cacgggacag
      841 acacttgccg tgctggtgat cggtgccgct ttcggcgcgg tgagggccgg catcaccatc
      901 gggctctacc tcgctcttgg cgcggccaac ctgccgcgcg ccttcgccga aggcaatacc
      961 gggatcgagt tcctaaaggc ggcctccccc accggcggct acctcgtcgg cttcctgatc
     1021 gccgcggtcg tggtcggcta tctggcgcag cggggctggg accggtccgt cggcagcgca
     1081 ctcggcgcga tgttcatcgg cgaggtcatc gtcttcactc ttggggtcgc gtggctcgcg
     1141 aacgctctcg atgttggggc tcagaaagcg atggagttcg gcctctatcc cttcatcatc
     1201 ggtgacgtga tcaagctcgc cctcgcggcg ggactgctcc cgaccgcgtg gagattcatc
     1261 aaggacaagg cgagcggacc caccttcaag aggtcttagc gctcggttcc cggatctcac
     1321 gcaggatcgg gagaccgaac caaaaccacg cgaagagccc ggcgaggatg cccgtcgcca
     1381 ccgcggtgat gttcgggaac gtgtaatcgg tcacgaaata gaccacgcag ctcatcgcgg
     1441 tcgccaggaa gaccgtgccg gcgatcgcga gcctgttgga gagcttgatc aagtgttctt
     1501 tgtcgcggtc acggaaccgg atccggtggt aggtcgtcgg cgagatgagc atgaggatag
     1561 ccaaggtcga aaacacgaac gccgcgtaat agacgccctt ctgggtcgcg gtcgtcagtg
     1621 cccacccctg gctgaaggga accatcagca ggaacgcgaa catcacctgc acgccgggca
     1681 gtgcgacgcg cagttcgttc agtaactcga tcagctcacg atcttgtttg ctctcgggca
     1741 tgtcagtgtc ctttagccca cgcgacgaga tcgtcgacgg acatgtagtt gatgactcgt
     1801 tccctgtcga tacccgcttt tattgccgct gcaaccccca tggggatgaa tcgcatctcc
     1861 atccggtcgt gggcatcggt ccctatcgag acacgaaccc cggcctcccg ggctatctcc
     1921 agcaactccg agttcaggtc ctgtcggtcg ggaaaggaat tgatctcgat cgctttgtcg
     1981 tgcttggtcg cctcctcgaa aacgcgcggc cagtcggcct ggagcccaag tcggttgtcg
     2041 tacttccgtc cgcgcgggtg cccgaggatc tggaagtgcg ggtttcgaac cgcggcaagg
     2101 tacctgtcgg tctggtcatc cttcgttcgc aacgacgagt ggaaggagcc cagcacgagg
     2161 tccaagggtt ccaacgactc cggctccata tccccctctc cctgagggtt gatgttcatc
     2221 tccagagccc ggaggacgcg gatgtcgtgc ccggccttgc gcagctcctc gttgaccaca
     2281 tcgatctcgt cgatctgagc gagaagctcc tcctcgtgca tgccaccggc gatcctcaga
     2341 cccttcgagt ggtcggtgat agcgatgtac tcgtagccgc gctcggcccc ctcacccgcc
     2401 atcgccacga tcgattcctt gccgtccgaa tattcggtgt gcatctggag atcaccgcgc
     2461 aggccggacg accactcggg gttgtcggca acgatcgcca gggcctccgc gaacgttctg
     2521 aagccggccc ggatcggagg ggggtcggga acctcgggcg gatcctcgag ccagcgcctg
     2581 atgatgccgc tcaaccagcc gccgatcttc ggaaggtccc ggagggagtc gccccgctcg
     2641 acgatatcgg cgacctcctc gggccagtgg tacgccgcgc gcgatgcact ccgaagggcg
     2701 cgttgtcggt gaccctcgtt ctcttctgcg gcacgagcca gaagctcggc cagctgtgca
     2761 ttggtaagca tgaaagaccc cttcgcaccg gagcctagtg ttccggtatg ccactcccgt
     2821 tcgacccgcc gatcgccccg atgctcgcga aactccagcc cgagatccct atcggcgagg
     2881 actggagata cgagccgaag tgggacgggt tccgggccat cgtcttcaga gacggatccg
     2941 agatccacat agtgagtcga gacgaacggc cattcgagcg ctatttcccc gaattgatcc
     3001 cattgttcaa ggagttcctt ccgaagacgt gcgtggtaga cggcgagatt gtgatccccg
     3061 ggaccgagaa gctggccttc gacgcgctgc tccttcggat ccacccggcc gcctcacgcg
     3121 tcaagatgct gtccgaaacg acccccgcaa gcttcgtggc gttcgacctc ctagcgcatg
     3181 gttcgaagga tctgcgcgag accccgctgg cgaaacgccg aaccttgctc gagaagtcac
     3241 tcggcggcta cgcgatcccc ggcgacgcga cgaaacggaa ggtgatgtcg gctcttcgac
     3301 ccgcgccgcg catcgcacta accccgcaca ccgacgatcc aaagcttgcg aagagatggt
     3361 tcgaagtctt cgagggggcc ggtctcgacg gagtgatcgc aaagaaagag gaccttcact
     3421 accgtcccgg cgatcgcgcc atggtcaaga tcaagcatcg ccgcacggcc gattgcgtgg
     3481 tcggcggata caggctctcg aagaccgggg acggtatcgg ttcgctattg cttgggctct
     3541 acgacgagac ggggagcctc cactacatcg gtcacacgtc cgcgttcaaa gccgacgagc
     3601 gaaaacgact gctgaaggac ttgaagaagc tcgagggcgg gtcgagcttc ggcgggggcc
     3661 gcgtgccggg agggccgagt cgatggaccg ggggccggga cgtgagctgg gtcccgatcg
     3721 agcccatact cgtgctcgaa gtctcgtacg actatctaca gggcaaccga ttccgccacg
     3781 cagcaggttt cgttcggtgg agagatgaca agaaaccgaa ggagtgcacc ttggatcagg
     3841 ttctctgagg agtgatcggg gaggcgatca catgttgtcg tttgtgatcg ccgaggcgat
     3901 cacaaacccc aacccttcga catgacgctc agccactgca tgagctcttc gcccggcatc
     3961 gggcggctgt ggaagaaccc ttgcgcgtag tcgcaaccga gctcctgcag cttcttccac
     4021 acgtcggcag tctctacacc ctccgccact acctgtaggc cgaggttgcg accgaggtcg
     4081 atcgtcgatt gcacgatcac gaggtcgctc tcgtcgacgg tcatgttcag cacgaaggac
     4141 ttgtcgatct tgagctccgc cagtggcatc cggcgcaggt acgacagcga cgagtatcca
     4201 gtcccgaaat cgtcgatgga cagcttgacg cccatctggc tcagccggct gagcacgccc
     4261 aatgcccgct tcgggtcgga aaccaacgtg ctctcggtga gctcgaactg gagcgacgac
     4321 ggggggacct gaagccgcct gagcgccccc tcgacgtgat cggggaagtc ctcgtccaac
     4381 aagctgcgag ccgacaggtt gaccgcaacg gggacctcca taccttcttc acgcaatttc
     4441 ttcgtttgct tgagggcctc gttgagga
//
LOCUS       DATMED010000249         4531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3641686, whole genome shotgun sequence.
ACCESSION   DATMED010000249 DATMED010000000
VERSION     DATMED010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641686"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VNP73_06410"
     CDS             complement(<1..287)
                     /locus_tag="VNP73_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWL65590.1"
                     /translation="MTADSIFRDLNDEQRRAVEATRGPVCILAGAGSGKTTTITRRIA
                     NQVATGSFEPGEILAVTFTDKAAGEMRSRLEALGTQGVKARTFHSAALVQLR"
     gene            complement(328..3015)
                     /locus_tag="VNP73_06415"
     CDS             complement(328..3015)
                     /locus_tag="VNP73_06415"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWL65591.1"
                     /translation="MTSFLGRQLRAGRIVVALAVLVGAVFAVSPARAAEEEIKIIRDK
                     LGVPHVYAPTASGASYGAGYVLAQDRLWQMHVLRRLAKGRLGEILGPIVAELDREARF
                     WTYTAEERAKRYETYPADIKDNLQAFADGVNAWMAAVRTNPAIVPLEFIKYQEQAALM
                     EDWTVDDSVALGDALILSFGSGGGNEIVYANLLDQLVKKFGKKKGKAAFGDLVMKSDP
                     DTVISIPKNHKYAQQPTAGRDSESQARRKLTQDARLSLQQEGSDSEAAASPSAPTAKG
                     TMEQLELLPDTDVALQGWLEHQDGLKMLRKIGGAIRFGSNATLAGPKFSKVGNSLQTG
                     GPQVGYYLPQILADIGIHGGNLDATGMTFAGAGPAVLIGRGNGYAWTTTTGASDLTDT
                     YVEKLNPDNNRQYQYKGEWEDMDCRTETYSTKGVPVEEQEICRTRHGPILAFDEANNV
                     AYSVRYAWMNRENQTVEGFWRYNSAKNLEDFATYSNYLSSNHNMFYADDQGNWGYWHP
                     GNHVRRAKGVDIRLPQDGTGGSEWRGLLPLKKVPHEVNSKRDWLMNWNNQPSFGWQRE
                     RAHPALDNALDLQHALDPAGPTLNDPYTGEPWNTDDKLEFEDMSGNLRYAAFKHHTHT
                     YFKRFLPKAKALKSELAKKMLETVKTYNGFRTSTEGDPWNVDGSYHAGFTIIERWVAN
                     MVPPAFEDDLAEVSAADAAGNATAGTSFAGQNLLWHVLNRRDRVDLRFDWLGKTSARA
                     LAAKAFEDTATALATEYENEDPATWTQTVTRQHYQRLNNEIFEDTGECEGGDCSGDSG
                     RPGDVADHIFMDRGTYNHVIEYLSAPSGSGIGNGDVAAGSVIPPGQSGFLPPQAVEGP
                     HYEDQLAYYIEWRYKPMPMTLVEATDLMESEETIVREVP"
     gene            3046..>4531
                     /locus_tag="VNP73_06420"
     CDS             3046..>4531
                     /locus_tag="VNP73_06420"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65592.1"
                     /translation="MTDSPVAGSFLLIEARYDLEALAKTTKKAPSKGADSQGFPTAHG
                     DAGPVDLRQLLGNLGPTVASVLTAPAGLDVPVGEPIIHDPVGGAPIEAQAIVLAVGTP
                     PDSTEARDLITSAARANAAVVAFKMHERDCRWLKEAEEAGIALVSVSDEVSWSHLNAL
                     LALTIPSLRQAGAIPGMASVPLGDLFALANAIAALVQGAITIEDQRGRVLAYSNIADQ
                     RIDEPRQFSILGRQVPDTPAVRETYKTLWASDSFIRVEMITGLDALPRIAAPITVGAQ
                     KIGSIWAIEGDIKLDASAEHALVEAARVAALHMIHARSSRDIERGIKGDLLRSLLEGR
                     GDVNATATRLGIPRRTPLIACGFELVADGPVEELHRERLVDLVATYSEAFRLQASSVA
                     IGRTVFCLLPVTKQMSHDRIREITKEIQEHASSTIGTPINAAIGSVVTDVRDVADASR
                     DVERILRVLSHDESRGQIASIEDVRSGVTLLTLRELARDHPDLVR"
BASE COUNT          768 a         1501 c         1410 g          852 t
ORIGIN      
        1 cggagttgca cgagcgcggc cgagtggaac gttcgcgcct tgaccccctg cgtgccgagg
       61 gcctcgagac gcgagcgcat ctcgcccgcg gccttgtcgg tgaaggtgac ggcgaggatc
      121 tcgcccggct caaacgaccc cgtcgctacc tggttcgcga tgcgacgcgt gatcgtcgtc
      181 gtcttgccgg acccggcccc cgcaaggatg cacaccggcc cccgagtcgc ctcgacggcg
      241 cgccgctgtt cgtcgttgag gtcgcgaaat atcgaatcgg ccgtcacttc cgcaccctaa
      301 aaggagggtg cgacgagccc gaaccgacta gggaacctct cggacgatcg tctcctcgga
      361 ttccatcaag tcggtggcct ccaccagagt catcggcatc ggcttgtagc gccactcgat
      421 gtagtacgcc agctgatcct cgtagtgtgg gccctcaacg gcctgcggcg gcaagaagcc
      481 actctgcccc ggcgggatca cgctgccggc ggcgacatcc ccgttaccga tgccactccc
      541 cgacggcgcc gacaggtact cgatcacgtg gttgtacgta ccgcggtcca tgaagatgtg
      601 gtcggcgaca tcgccggggc gaccggagtc gcccgaacaa tcgccgccct cgcactcgcc
      661 ggtgtcttcg aagatctcgt tgttcagcct ctggtagtgc tggcgcgtaa cggtctgcgt
      721 ccacgttgcc ggatcttcgt tctcgtactc ggtcgcgaga gcggtcgccg tgtcctcgaa
      781 cgccttagcg gcgagagcgc gcgccgacgt cttgcccagc cagtcgaagc gcagatccac
      841 acgatccctg cgattcaaca cgtgccacag caggttctgg ccggcgaacg acgtaccggc
      901 ggtcgcgttc cctgccgcat cagcggcact cacttcagcg aggtcgtctt cgaacgccgg
      961 cggcaccatg ttcgccaccc accgctcgat gatcgtgaag ccggcgtggt agctgccatc
     1021 tacgttccac ggatcgccct cggtcgaggt tcggaacccg ttgtaagtct tgacggtctc
     1081 cagcatcttc ttggccagct cactcttcag cgctttcgcc ttcggcaaga acctcttgaa
     1141 gtacgtgtgc gtgtggtgct taaacgcggc gtagcgcagg ttgccggaca tgtcctcgaa
     1201 ctcgagtttg tcgtcggtgt tccacggctc gccggtgtac gggtcgttca gggtcgggcc
     1261 cgcaggatcg agcgcgtgct gaaggtcgag cgcgttgtcg agcgcaggat gggctcgctc
     1321 gcgctgccac ccgaacgacg gctggttgtt ccagttcatc aaccagtcgc gcttggagtt
     1381 cacctcgtgc ggcaccttct tcagtggcag cagtccgcgc cactccgatc ccccggtgcc
     1441 gtcttggggc agccggatat ccacgccctt cgcgcgccgg acgtgattgc ccgggtgcca
     1501 gtagccccag ttcccctggt cgtcggcata gaacatgttg tggttcgagg acaggtagtt
     1561 cgagtaggtc gcgaagtcct cgaggttctt cgccgagttg taacgccaga agccctcgac
     1621 ggtctggttc tcacggttca tccacgcgta acgcaccgaa taggcgacgt tgttggcctc
     1681 gtcgaacgcg aggatcgggc cgtggcgggt gcggcagatc tcctgctcct cgaccggcac
     1741 acccttcgtg ctgtaggtct cggtgcggca gtccatgtcc tcccactcgc ccttgtactg
     1801 gtactgccgg ttgttgtcgg gattgagctt ctcgacgtac gtgtcggtga ggtccgacgc
     1861 accggtcgtc gtcgtccacg cgtagccgtt gccgcgcccg atcagcacgg cgggaccggc
     1921 ccccgcgaac gtcatccccg ttgcgtcgag attgccgccg tggataccga tgtcggcgag
     1981 gatctgcggg aggtagtagc cgacctgggg cccgccggtc tgaagcgagt tgccgacctt
     2041 cgagaatttc ggcccggcca gcgtcgcgtt cgaaccgaac cggatcgctc cgccgatctt
     2101 gcgcagcatc ttgaggccgt cctggtgttc gagccatccc tgtagcgcga cgtctgtgtc
     2161 gggcagaagc tcgagctgtt ccatcgttcc cttagcggtt ggagccgacg gagacgccgc
     2221 tgcctcagaa tcactcccct cctgctgcaa gctgaggcgc gcgtcctgcg tcagtttccg
     2281 gcgcgcctgg ctctcgctat cgcgtccggc cgtcggttgc tgtgcgtact tgtggttctt
     2341 ggggatcgag atgaccgtgt cgggatcgga cttcatcacg aggtcgccga acgcggcttt
     2401 gcccttcttc ttgccgaact tcttgacgag ttggtcgagc aggttcgcgt agacgatctc
     2461 gttaccgcca cccgaaccga acgacaggat caaggcgtcg ccgagcgcga cgctgtcgtc
     2521 gaccgtccag tcctccatca acgccgcttg ctcctggtac ttgatgaact cgagaggcac
     2581 gatcgcggga ttcgtgcgca ccgcggccat ccacgcattc acaccgtcgg cgaaggcctg
     2641 caggttgtcc ttgatgtccg cgggataagt ctcgtagcgc ttcgcacgct cctcggccgt
     2701 gtaggtccag aaccgagctt ccctatcgag ctcggccacg atcggaccga ggatctcgcc
     2761 gaggcggccc ttggcgagac gacgaagcac gtgcatctgc cacagacgat cctgcgccag
     2821 cacgtaaccg gcgccgtaag acgcgcccga agcggtcggc gcgtacacgt gcggaacgcc
     2881 gagcttgtcg cggatgatct tgatctcttc ttcggcggcc cgggccggcg acaccgcaaa
     2941 cacggccccc acgaggaccg caagcgccac gacgatccgc ccggcacgca actgacgacc
     3001 caagaacgaa gtcattgacc ccacctcttg acttagatgt cctaaatgac cgattcgccg
     3061 gtagcggggt cattcctctt aatcgaggcc cgctacgatc tggaagcttt ggcgaagacg
     3121 acaaaaaagg ccccctcaaa aggtgccgac tcacagggct tcccgactgc gcatggcgac
     3181 gcgggcccag tcgacctgcg ccagctactc ggcaatctgg gccccaccgt tgcaagcgtc
     3241 ttaacggcgc cggccggtct cgacgtcccg gtcggagagc cgatcatcca cgacccggtc
     3301 ggcggtgccc cgatcgaagc ccaagcgatc gtccttgcgg ttgggacgcc ccccgattcg
     3361 accgaagcgc gcgacctgat cacatctgca gcgcgagcga acgccgcggt cgtcgccttc
     3421 aagatgcacg agcgcgactg tcgctggttg aaagaagcgg aagaagccgg catcgcgctc
     3481 gtctcggtgt cggacgaggt gagttggagc cacctcaatg cactgcttgc gctcacgatc
     3541 ccatcactgc ggcaagcggg agccatcccg ggcatggcaa gcgttccgct gggggacctc
     3601 ttcgcactcg ccaatgccat tgcggctttg gtccaaggcg cgatcacgat cgaagaccag
     3661 cgcggtcgtg tcctcgccta ctcgaacatc gccgatcagc gcatcgacga gccgcgccag
     3721 ttctcgatcc tcggacgcca ggtacccgat acgccggcgg tgcgcgagac atacaagacg
     3781 ttgtgggctt ccgactcgtt cattcgagtg gaaatgatca ccggactgga tgcgctcccc
     3841 cggatcgcgg cccccataac cgtaggcgct cagaaaatcg gatcgatctg ggcgatcgaa
     3901 ggcgacatca agctcgacgc ttcggccgag cacgctctgg tcgaagcggc tcgggtggcc
     3961 gcgctgcaca tgatccacgc acgctcgtcg cgcgacatcg aacgaggcat caagggagac
     4021 ctcttgcgtt cattgctgga agggcggggc gatgtgaacg cgacggcgac tcggctcggt
     4081 atccctcgac gcacgccgtt gatcgcatgc gggttcgagt tggtcgccga cgggccggtc
     4141 gaagagctcc atcgcgaacg actggtggat ctcgtcgcta cctactcgga ggcattccgg
     4201 ctccaagctt catcggttgc gatcggccgt acggtcttct gcctcctgcc ggtgacgaag
     4261 cagatgagcc atgaccggat ccgagagatc acgaaggaga tccaggaaca cgcaagcagc
     4321 acgatcggca cccctatcaa cgccgcgatc gggtcggtcg tgaccgacgt gagggacgta
     4381 gcggacgcca gccgcgatgt agaacggatc ttgcgcgtcc tcagtcacga cgagagccgg
     4441 ggtcagatcg cctcgatcga ggacgtccgc agcggggtca cccttctgac gctgcgtgag
     4501 ctcgctcgag accatcccga tctcgtacgc g
//
LOCUS       DATMED010000250         4602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4037963, whole genome shotgun sequence.
ACCESSION   DATMED010000250 DATMED010000000
VERSION     DATMED010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4037963"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..930
                     /locus_tag="VNP73_06425"
     CDS             265..930
                     /locus_tag="VNP73_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65593.1"
                     /translation="MDILERLRVLIANQKDDRLELLATVIEDMGHEVVARSITVSEVA
                     ALTARQLPDVAIVGLGESSEHALGLISEIVRESYCPVIAILSDFDEAWVNEAAKRGVY
                     AYILDGHPDELQSAINVTLNRFADAQSLQSAIERRNAESARQNENTNARRRQALELHD
                     GVVQGLVAAQLAHDLGRDEESRAALVSTLERAKAVVSRSLQELKEEGLDSEQLIRASS
                     TDE"
     gene            923..1348
                     /locus_tag="VNP73_06430"
     CDS             923..1348
                     /locus_tag="VNP73_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65594.1"
                     /translation="MSKESASSNGLIGVLICDDQEDVRLLLRMVIGQKRDMGGGRGLE
                     VVGEARDGDEAVSEAKRLQPDVILLDLSMSHRTGLEALPEIKEVAPAARVIVFSGFEA
                     SIMASDVLSRGADRYLEKGTHPSEITAAIEEVAALGQPA"
     gene            complement(1369..2502)
                     /locus_tag="VNP73_06435"
     CDS             complement(1369..2502)
                     /locus_tag="VNP73_06435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWL65595.1"
                     /translation="MTTWKEVAVDAAEETAAPPRGSRTLEMLGITNDDLVLMDGDAVL
                     SETLRSTHDLLHALKRDHVLDTFLQLPMIDRSNFLNWIASSTDRDVRRSRTRSFVSAL
                     RSSPLASKTRENRAIAVTEPSSAELEQPTRIGVDRDQIADDRDAAADAHDEASRARDE
                     KAQARDERAEGRERELDQIDAGAAADRAGALRDRRAAVTDRREAGDDRAAAAADREVS
                     AREREVSSIDELTGAYRRDAGTLALERELSKAKRMDRSLVVAFVDVDDLKQTNDSLGH
                     AAGDLRLRETVRAMRKRLRSYDLIVRFGGDEFVCGMPEASFSTAVQRFAAVNTVMGRA
                     GHGSITVGLAEMGSGDTLEGLIARADEALYLQRDRALSLQTPD"
     gene            complement(2499..2768)
                     /locus_tag="VNP73_06440"
     CDS             complement(2499..2768)
                     /locus_tag="VNP73_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWL65596.1"
                     /translation="MSEDQTITVEDLLDAMAEADVLETFCQLPQEDQENFSRWIGMAR
                     DDESHWRRIHAFVSALRTGPFEPIALVEPALLFGNPEAPPLGAQG"
     gene            complement(2765..2968)
                     /locus_tag="VNP73_06445"
     CDS             complement(2765..2968)
                     /locus_tag="VNP73_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65597.1"
                     /translation="MDISLIPDEVKSLITVLKGQIFLLGKDWDNLLEPEKKEVSQSAV
                     KTISELNDMVRGAPEPPALRVLS"
     gene            complement(3136..3618)
                     /locus_tag="VNP73_06450"
     CDS             complement(3136..3618)
                     /locus_tag="VNP73_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65598.1"
                     /translation="MGPDRKKQFLKKNSQLVPSDENVLAVIIAEGKGGAWRRGLAAGI
                     SSASPLAGAVADRVESVGAKDPKVGSATAWPEASIFWLAFTDKQLHVFEGQVNSQQAG
                     PGAAHYPFDGISGMRFEKKLLISKLTVSFADGSSVELDIAKQNIKPFVEAIENKFASV
                     "
     gene            complement(3735..>4602)
                     /locus_tag="VNP73_06455"
     CDS             complement(3735..>4602)
                     /locus_tag="VNP73_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWL65599.1"
                     /translation="PAMRGWMNEGRSYVPPVGIGEVMRALAAGRVIESEHSGFEGGDT
                     VIGTFGVQEYAVSNGAMVMKVDPELAPLPVYLGTLGITGLTAYFGLLDVGALKEGETV
                     VVSGAAGAVGMVAGQIAKIKGCKVVGIAGGPEKCEFIRTELGFDAAIDYKSDDIRAAL
                     KEHAPKGVDVYFDNVGGEILDTVLTRLARHARIVICGAISQYNNDGPMRGPSNYMSLL
                     VNRARMEGFVVFDYADRYLEAIIEMAGWMNAGKLKTREDIVEGLDNFPETLLKLFRGE
                     NTGKLVLQVASE"
BASE COUNT          882 a         1441 c         1368 g          911 t
ORIGIN      
        1 tccgcgtcgg ttcggcccgc gtagggctgg ccttcacgag ttccaacgat gcaaccgccg
       61 tggcccttac acacaggcaa ggcgacgtcc gggtgacgat ccatcaggag tagaagatcc
      121 tgaatgcgta atcaggttag ctgcgggaat ggatcagcac gtatgtcgct cccgcccgat
      181 ttgggagccg ctgaaacctg gcgcacttca ttcaaccggg gcgttcattg attaaaatgg
      241 tgaagattcg tctcttggaa ggcggtggat atcttggagc gtcttcgggt tctgatcgcc
      301 aaccagaaag acgatcggct cgagctgctg gcgaccgtta tagaggatat gggccacgag
      361 gtagtcgccc gctcgataac ggtatcggaa gtcgcggctc taacggctcg ccaactcccc
      421 gatgtcgcaa tcgtcggcct cggcgagagt tccgagcatg ccctcgggct catctcggag
      481 atcgtgcgcg agtcctactg tcccgtgatc gcgatcctca gcgacttcga cgaggcctgg
      541 gtcaatgagg cggccaaacg aggtgtctat gcctacatct tggacggaca tccggacgag
      601 cttcagagcg cgatcaacgt caccttgaat cgttttgccg acgcccagag tttgcagagc
      661 gccatcgagc gtcgcaacgc tgagtccgct cgacagaatg agaatacgaa cgcccggcgg
      721 cggcaagcgc tggagcttca cgacggtgtt gttcagggcc tagttgccgc ccagctcgcg
      781 cacgacctcg ggcgcgatga ggagtcccgc gccgctctcg tctcgactct tgagcgcgcc
      841 aaggcggtgg tcagccgctc gctccaagag ctgaaggaag aagggctcga ctccgagcag
      901 ctgatccgtg cctcttcaac cgatgagtaa agagtcggca tcatcaaacg ggttgatcgg
      961 cgtactgatc tgcgatgacc aggaggatgt gcgattactt cttcggatgg tcatcgggca
     1021 aaagagggac atgggagggg gaagaggtct cgaagtcgta ggtgaggcgc gagacgggga
     1081 cgaagccgtc tccgaggcga agcggctcca gccggacgtg atccttcttg acctctcaat
     1141 gtcgcatcga acgggcctcg aagctctacc cgagatcaaa gaggttgccc ctgccgccag
     1201 agtcatcgtc ttttccgggt ttgaggcctc gattatggcc tccgacgttc tgtcacgagg
     1261 cgccgatcgc tatttggaga aagggacaca tccgagcgag atcacggcag cgatcgagga
     1321 agtcgcagca ctgggccaac cggcttagcc catcatccgc gacggacgtt aatcaggcgt
     1381 ttggagggat agggctcggt cacgctggag gtagagcgcc tcgtcagcgc gggcgatgag
     1441 cccctcgagt gtgtcccccg aacccatctc cgctaggccc accgtgatcg acccgtgacc
     1501 agcgcgaccc atgaccgtgt tgacggctgc gaatcgctgc accgcggtcg agaagcttgc
     1561 ctccggcatg ccacagacga attcatcgcc tccgaaccga acgatcaggt cgtacgaacg
     1621 aagacgtttt cgcatcgccc gaaccgtctc gcggagacgc aggtcgccgg ctgcgtgccc
     1681 tagagagtca ttcgtttgct tgaggtcatc cacatccacg aacgcaacga ccaaagaccg
     1741 atccattcgc tttgctttgc tcagctcgcg ctcgagcgcc agcgtgcccg cgtcgcgtcg
     1801 gtaagcgccg gtaagctcgt cgatcgacga cacttccctc tcccgagcag aaacctcgcg
     1861 gtcggctgcg gcggcggcgc gatcatctcc cgcttcacgc cgatccgtga cagccgcgcg
     1921 acgatcacgc aacgcccccg cccgatcggc agctgctccc gcgtcgatct gatccaattc
     1981 tcgctctcgg ccttcggccc gctcgtcgcg agcttgagcc ttctcatcac gagcgcgaga
     2041 tgcttcgtca tgggcgtccg ccgctgcatc tcggtcgtcg gcgatctggt ctcggtcgac
     2101 gccgatccgc gtcggctgct cgagctctgc actgctcggc tcagtaacgg cgatcgctcg
     2161 gttctcccgg gttttgctcg cgagaggaga acttcttaat gccgacacga acgacctcgt
     2221 tctactgcgg cggacgtcac ggtccgttga tgaggcgatc cagttgagga aattcgatcg
     2281 atcgatcatc ggcagctgca ggaaggtatc gagaacatga tcacgcttga gtgcatggag
     2341 caggtcatga gtgctccgca acgtttccga caggactgca tcgccgtcca tcagcaccaa
     2401 gtcgtcgttg gtgatcccca gcatctcaag ggtccttgaa ccccttggcg gggcggccgt
     2461 ttcctcggcg gcgtctacgg caacctcctt ccaggtggtc atccttgggc ccctagtggg
     2521 ggggcttcgg gattgccgaa cagaagcgcc ggttccacga gtgcgatggg ctcgaagggg
     2581 ccggttctca aggccgagac gaaagcgtga atccgccgcc agtgcgattc gtcgtctcgc
     2641 gccatgccga tccagcgcga gaagttctct tgatcctctt ggggaagctg gcagaacgtc
     2701 tctaaaacgt ccgcctcagc catcgcgtcc agaaggtctt cgaccgtgat cgtctggtct
     2761 tcgctcatga aagcacccgc aaggcaggag gctcgggcgc cccgcggacc atgtcgttga
     2821 gctctgagat ggtttttacc gcggattggg acacctcctt cttctccggt tccaggaggt
     2881 tgtcccagtc tttgccgagg aggaagatct ggccctttaa gacggtgatg aggctcttga
     2941 cctcatcggg aatgaggctg atatccatgt gacggtgccc ttcccgtcaa cggaatcggc
     3001 cgaggtgacc attgtgccct gaggccgttg gcgattgatg taactccacc caccgtactc
     3061 ctaatcgggc gagggggcga gagccaacac ttggctttta cgagacacct gtctcgctca
     3121 tgacgccgat aactcctaaa ccgacgcgaa cttgttctcg atggcctcta cgaacggctt
     3181 gatgttctgc ttagcgatat ccagctcgac agacgagcca tccgcgaaag acacggtcag
     3241 tttcgatatc aagagcttct tttcgaagcg cataccggag atgccatcga acgggtaatg
     3301 cgcagctccg gggccggctt gctgcgagtt cacctggccc tcgaagacat gtagttgctt
     3361 gtcggtgaac gcgagccaaa agatcgaagc ctccggccac gccgttgccg agcccacctt
     3421 cggatccttc gccccaaccg attcaacgcg atccgcgacc gcgccggcta agggagatgc
     3481 acttgagatt ccggcagcga ggccgcgtcg ccatgccccg cctttccctt ccgcgatgat
     3541 cacggcaagg acgttctcgt cggagggaac gagctgagag ttcttcttga ggaattgctt
     3601 cttgcgatcg gggcccaatg gcttctcctc tcggatggaa gccgcagcac aaacgacctt
     3661 agctccacgt gtaagtggtg ccaaggacct tgggcccaac ccgatggggt cccgtagcgg
     3721 gggccgtcgg gtggctactc cgaagcgacc tgcaggacca gcttgcccgt gttctcgccc
     3781 ctgaacagct tcaggagggt ctccgggaag ttgtcgaggc cctcgacgat gtcctcacgc
     3841 gtcttcaact tcccggcgtt catccaaccc gccatctcga tgatcgcctc gagataccgg
     3901 tcggcatagt cgaacaccac gaacccttcc atccgggccc ggttcacgag caatgacatg
     3961 taattagaag ggcctctcat cgggccatcg ttgttgtact gcgagatcgc tccgcagatg
     4021 acgatgcggg catgccgcgc cagccgggtg aggaccgtgt cgaggatctc gccgccgacg
     4081 ttgtcgaaat agacgtctac gcccttggga gcatgctcct tgagtgcggc tctgatgtca
     4141 tcggacttgt agtcgatcgc cgcgtcgaag ccaagctcgg tgcggatgaa ctcgcacttc
     4201 tccggccccc ccgcgatgcc gaccaccttg cagcctttga tcttggcgat ctgcccggcg
     4261 accatcccaa ccgcgcccgc agctccggac acgacgaccg tctcgccttc cttcaatgca
     4321 ccgacatcca gcagtccgaa gtacgcggtc agaccggtga tcccaagcgt cccgagatag
     4381 acgggaagcg gagccagttc ggggtcgacc ttcatgacca tggctccgtt ggagaccgcg
     4441 tactcctgaa cgccgaacgt accgatcacc gtgtctcccc cttcgaaacc ggagtgctca
     4501 gactcgatca cccgacctgc agccagagcg cgcatgactt caccgatccc gacgggaggc
     4561 acgtaggaac gaccttcgtt catccagccg cgcatcgcag ga
//
LOCUS       DATMED010000251         3410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4038043, whole genome shotgun sequence.
ACCESSION   DATMED010000251 DATMED010000000
VERSION     DATMED010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038043"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VNP73_06460"
     CDS             <1..1624
                     /locus_tag="VNP73_06460"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HWL65600.1"
                     /translation="NDDNFNLQIDPAEENTTPQVVDEDGNVKGLVAIVPGHVFGDLYR
                     DVPFPGGIMNVTFAAGWSAGRIYEPFQAPLEDQFNKQEVDPQCTRNTADHVANPPQNP
                     FVKALLPVNQYDGALFKERSPWYWADNIEVPTFLIEAWQDEQVGSRATELIERFDEDL
                     EWRALFTNGDHGEYYGSHILPHIDRFLRYHLETPIDPREIGTVTEEVSDPDPTPSPTP
                     SPTATLPNGKPRKPKGKPSPTPSPTPTPTPSPSTTTRDETQAEALARYNAEPPVQINY
                     ETGAGGNRVPAWSETYASWPPPNQEVWRLNMTADGKLVDPDTAYDSAPSVTYRYEPGQ
                     SQQRGGYKLVGDLPDETEPQWSEHPLLGTYAMFQTDVLDSDKVLAGPASVDMWVSSTA
                     ADTDFQVTVTEIRPESGKEVFVQQGWLRASHRFLDEEQSTALRPYQTHAPTDVLPLVP
                     GLPEQVRLEIFPFAHAFRAGSKIRIYVEAPHVKPDLWGFALLPVPAMNTIYTNATQPS
                     SVALPLLDGAEAKADALSCSLRNQPCRDLLVP"
     gene            1652..3058
                     /locus_tag="VNP73_06465"
     CDS             1652..3058
                     /locus_tag="VNP73_06465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL65601.1"
                     /translation="MRVRQSFVSAVIAGLIAGALLLPSPYAGASHAPGPKGDPSGPNA
                     VAHNAGGKVLKSAKLAHRAPDAVYNDTGFDAVEPSLGMTKQGNVFYAASGTRNEVIRS
                     KDGERWDVVSPRLGDQNIHAITLDPYVYVDEWTNRVYTIDLTVACSLMSYSDDQGETW
                     TTNPLACGRPVNDHQTLFSGPPVSSPTVAYDNIVYYCWNDVATSSCGKSLNGGLTWTP
                     TGSPVFHAGDGTEGQDLPRDCGGLHGHGVVGQDGTVYLPKEHCTEPWLAISRDEGQTW
                     EHVRVANKVAIWGPDPSVAVDDKGNIYYTFLHKDRLPYITYSRDGGKTWSEPLMFGRP
                     GLTEAALATMDVGGPGKVAMAYYGTDDVDGKIDKRKYPTKVVWHGYMTTTDDLFAKDP
                     VFYSGNVNTSKDPLARGACGPRRCYEAYDFIDVIVGFDGVAWAAFADSCVAICINGET
                     LGGHTGGVARMVGGPSLK"
     gene            3095..>3410
                     /locus_tag="VNP73_06470"
     CDS             3095..>3410
                     /locus_tag="VNP73_06470"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HWL65602.1"
                     /translation="MKTLVALVVVAILLVAGDAAARSYASSELEAQLTSALRLEGQPR
                     VDIDGFPFIVRLVQGSFDEVQISARGVRSPEIELSRAKVTLNDVRFSTSKALSGDLRA
                     IDF"
BASE COUNT          667 a         1122 c         1045 g          576 t
ORIGIN      
        1 gaacgacgac aacttcaacc tgcagatcga tcccgcggaa gagaacacca cgccccaggt
       61 cgtcgacgag gacgggaacg tcaagggcct cgtagcgatc gtgccgggcc acgtgttcgg
      121 ggacctctac cgagacgtcc ccttccccgg cgggatcatg aacgtcacct tcgcagcggg
      181 atggtccgcc ggtcggatct acgaaccgtt ccaggcgccg ctcgaggatc agttcaacaa
      241 gcaagaggtc gaccctcagt gcacgcgcaa cacggccgat cacgttgcca atccgccgca
      301 gaaccccttc gtgaaggctc tccttcccgt gaaccagtac gacggcgccc tcttcaagga
      361 gcgctcaccc tggtactggg ccgacaacat cgaagttccg acgttcctta tcgaagcgtg
      421 gcaggacgag caggttggct cgcgtgcgac cgagttgatc gaacggttcg acgaggacct
      481 cgaatggcgg gcgctgttca cgaacggcga ccacggcgag tactacggga gccacatcct
      541 tccgcacatc gatcgtttcc tccgctacca cttggagacc ccgatcgacc cccgcgagat
      601 cggaaccgtg accgaggaag tgtccgatcc ggacccgacc ccgtcgccca ctccttcgcc
      661 gaccgcgacg ttgccgaatg gcaagccgcg caagccgaag ggcaagccct ctccgacgcc
      721 cagtccgacc cccacgccta ctccgtctcc gtcgaccacc acccgggacg agacgcaagc
      781 cgaggcactg gcccgttaca acgccgaacc cccggtgcag atcaactacg agaccggtgc
      841 cggcgggaac cgcgtcccgg cttggtccga gacctatgca tcgtggccac ctcctaacca
      901 agaggtgtgg cgactcaaca tgacggccga tggcaagctc gtcgatccag acacggctta
      961 cgacagtgca ccgagcgtca cttatcgcta cgagccgggt cagtcccagc agcgcggcgg
     1021 ttacaagctc gtcggcgatc tgccggacga aaccgaacca cagtggagcg agcatcccct
     1081 cctcgggacg tacgccatgt tccagaccga cgtgctcgat tccgacaagg ttctcgccgg
     1141 accggccagc gtcgacatgt gggtgtcttc cacagcagca gacacggact tccaggtgac
     1201 cgtcacggag attcgtcccg agagcggcaa ggaagtcttc gtgcagcagg gctggctacg
     1261 tgccagtcac cgcttcctgg atgaggagca atcgacggct ctgcgtccgt accagacaca
     1321 cgctcctacc gatgtcctgc cgctggtccc aggactaccg gagcaggtac ggctcgagat
     1381 cttcccgttc gctcacgcat tccgggccgg ttccaagatc cggatctacg tcgaggcacc
     1441 gcacgtgaag cccgacctgt ggggcttcgc cctcctgccg gtgccggcga tgaacacgat
     1501 ctacacgaac gcaacgcagc cgtcttccgt cgcacttccg ttgctcgacg gtgcggaggc
     1561 caaggcggat gcgctctcgt gctcgcttcg caaccagccg tgccgtgacc tattggtgcc
     1621 ctaacggatc gacgacaagg gggtcgtgtc tgtgagagtt aggcagtcgt tcgtatcggc
     1681 ggtgatcgcc ggtctgatag caggagctct tcttcttccg tcgccgtatg caggcgcgag
     1741 ccacgctccg ggtccgaagg gagacccttc cggccccaat gcggtggctc acaacgccgg
     1801 cggcaaagtg ctgaagtcgg cgaagctcgc tcaccgggcc cccgacgccg tctacaacga
     1861 caccggattc gacgcagtcg agccgtcgct cgggatgacc aagcaaggaa acgtcttcta
     1921 cgccgcgtcc gggacgcgca acgaggtgat ccgcagcaaa gacggcgaga ggtgggatgt
     1981 ggtctctccg aggctcggcg accagaacat ccacgcgata actctcgacc cttacgtcta
     2041 cgtcgacgaa tggacgaacc gggtctacac gatcgatctg accgtggcgt gctcactcat
     2101 gagctacagc gacgatcagg gtgagacctg gacgacgaat cccttggcct gtggccgccc
     2161 ggtcaacgat caccaaacgc tgttctcggg accgcccgtc tcaagcccca cggtcgccta
     2221 cgacaacatc gtctactact gctggaacga tgtcgcgact tcgtcgtgcg gcaagtcgct
     2281 gaacggcggc ttgacatgga ccccgacggg gagccctgtg ttccatgccg gcgacgggac
     2341 cgagggccaa gaccttcctc gtgattgcgg cggactgcac ggccacggcg tcgtcggaca
     2401 ggacggcacc gtctaccttc cgaaggagca ttgcaccgag ccgtggctgg cgatcagccg
     2461 cgacgaaggc cagacgtggg agcatgttcg cgtcgccaac aaggtggcga tctgggggcc
     2521 ggacccgagc gttgcggtcg acgacaaagg caacatctac tacacgttcc tgcacaagga
     2581 ccgtctgccg tacatcacct actcccgcga tgggggcaag acgtggagcg agccgctgat
     2641 gttcggacgg ccgggtctca cggaagccgc tcttgccacg atggatgtag ggggccccgg
     2701 caaagtggcg atggcctact acggcaccga cgacgtcgac ggcaagatcg acaagcgcaa
     2761 gtacccgacg aaggtggtgt ggcacggata catgaccacc acggatgacc tgttcgccaa
     2821 ggacccagtg ttttactcgg gcaacgtgaa cacatcgaag gacccactgg cgcgcggcgc
     2881 atgcggcccc cgccgttgct acgaggccta cgatttcatc gatgtcatcg ttgggttcga
     2941 cggagtggcg tgggccgcgt tcgccgactc gtgcgttgcc atatgcatca acggcgagac
     3001 cctcgggggc cacacgggtg gagttgcccg catggtcggc gggccgagtc tcaagtagta
     3061 gcagctcgcc caaatcccag aggaacataa gaccgtgaag acactcgtcg cgttagtcgt
     3121 cgttgccatt ctgttggtgg ccggcgacgc ggccgcccgc tcgtacgcgt cctcggagct
     3181 cgaggctcaa ctaacgtccg cgctccgcct cgaggggcaa cctcgcgtcg acatcgacgg
     3241 gttcccgttc attgtgagac tcgtccaggg cagcttcgat gaggtccaga tctcggctcg
     3301 aggcgttcgc tcgccggaga tcgagttgag cagagcgaag gtcacgctta atgacgtgcg
     3361 cttctctacc tcgaaggcgt tgagcgggga tctacgtgcg atcgatttct
//
LOCUS       DATMED010000252         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_571085, whole genome shotgun sequence.
ACCESSION   DATMED010000252 DATMED010000000
VERSION     DATMED010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571085"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..861)
                     /gene="folP"
                     /locus_tag="VNP73_06475"
     CDS             complement(28..861)
                     /gene="folP"
                     /locus_tag="VNP73_06475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWL65603.1"
                     /translation="MSIAGRCLVMGIVNRTPDSFYDGGRMDLTESVAHAHSLRQQGAD
                     LIDIGAVKAGPGEEVPEEEELERVIPLVTEVAQFASGRISIETASPLVAEKAFEAGAS
                     VLNDVSELLDARLAKVCAQAGAGLVVMHNGGQIRGRPRHPVYEDIVATVLECWEALTT
                     KALAAGIPRERIVVDAGLDFGKTTFHSLELVRRMDEQVATGWPVLVAPSRKDVVGETL
                     ELPPEERLEGSLALAVLAAYMGAAIVRVHDVRATARAVHMAESVSRRRSPGSPVRGLW
                     D"
     gene            complement(967..1857)
                     /locus_tag="VNP73_06480"
     CDS             complement(967..1857)
                     /locus_tag="VNP73_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWL65604.1"
                     /translation="MAGAATIQQRLLSPWPILVAGVLAASVSGILVRYADGVHPLAIS
                     FWRCAAGAAFLAPFARKRFSRLRAVDLKLPAVAGVFLALHFATWIWSIGLTSIAASVL
                     LVSTTPVFSGIAAWFLLKERLRSAAWVGIALALFGTGLIGGGDLSGSNLLGNALALLG
                     GIAAAGYVLSGQVARQDLGLLEYAVVTYAVAAVALLIACLAAGADLLGYPQSSWWALL
                     AIVVGPQLLGHTLINQVLRDIDATTVSVVVMVEPLIATWLAYVLFAEVPSALIYPGGV
                     AVLIGMYLVSAHRREPAVIK"
     gene            1899..2300
                     /locus_tag="VNP73_06485"
     CDS             1899..2300
                     /locus_tag="VNP73_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWL65605.1"
                     /translation="MSDGPPTVAVGAIVVDDGALLMVKRGHEPAKGLWSVPGGRLERG
                     EYMTAAVKREVKEETGLDIEVGGLVGFLEVLGDQHYVILDFAARVDGERTPNAGADAA
                     EARWIPLEQIEELECTPRFVETLKSWGVLPA"
     gene            complement(2309..>2600)
                     /locus_tag="VNP73_06490"
     CDS             complement(2309..>2600)
                     /locus_tag="VNP73_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWL65606.1"
                     /translation="NKIEVGVVMATDPPPGGMLSEGSHIEVQVSAGPRYQKVRMPDVR
                     GMSVEAAQSRLSSLGLRVRVEESCEGGSTVVETHPIPGTMLKENDLVALFVC"
BASE COUNT          505 a          887 c          803 g          405 t
ORIGIN      
        1 gagttgccga ggcgatcatc gcctggatca gtcccacaat cctcgaacgg gggacccagg
       61 agaacgccgc ctggagaccg actccgccat atgaacggct ctggcggtag ctctaacatc
      121 gtggacccgg acgatcgctg cccccatata ggccgctagc accgccagcg ccagactccc
      181 ctccaaccgc tcctccggcg gaagctccag cgtctccccc actacgtctt tacgtgacgg
      241 cgccaccagg accggccaac ccgtggccac ctgctcgtcc atccgtcgca ccagctcgag
      301 cgagtggaac gtcgtcttac cgaaatccag tcctgcgtcg accacgatcc gctcgcgcgg
      361 gatcccggcg gcgagcgcct tggtggtcaa cgcctcccaa cactccagca cggtggcgac
      421 gatgtcttcg tagacgggat ggcgcggacg gccccggatc tggcccccgt tgtgcatcac
      481 gacgagaccc gcacctgcct gggcgcagac cttcgccagg cgtgcgtcga gcagttcgct
      541 gacgtcgttc agaaccgagg cccccgcctc gaaagccttc tcggccacca gggggctcgc
      601 cgtttcgatc gagatccgac ccgacgcgaa ctgagcgacc tccgtgacga gcgggatcac
      661 ccgttcgagc tcctcttcct cgggtacctc ctcccccggt cccgccttga ccgccccgat
      721 gtcgatcaga tccgcgccct gttggcgcag ggagtgggcg tgagctaccg actcggtgag
      781 atccatccga ccgccgtcgt agaaggagtc cggcgtgcgg ttgacgatcc ccatgacgag
      841 gcagcgcccg gcgatgctca tttgtcctcc ctcaggtatg cccgccacgc gggcagccct
      901 tcgcttcgga cggcgcgatc gtcgagacga tcgctcattt gtcctcgtga tcgtcgagac
      961 gatcgctcat ttgatcaccg cgggctcgcg tctatgagcc gagacgagat acatcccgat
     1021 cagaaccgca acacctcccg gatagatcag cgcagaggga acctcggcaa agagaacgta
     1081 cgccagccag gtagcgatca ggggctcgac cataacgacg accgaaaccg tcgttgcatc
     1141 gatgtctctg agcacctggt tgatcagcgt gtggccgagc aattgggggc ccacaacgat
     1201 cgcgagcagg gcccaccacg aactttgtgg gtagcccaac agatccgcgc ccgcggcgag
     1261 acatgcgatc agcaacgcga ccgcggcaac cgcatacgtg acgacggcgt actcgagcag
     1321 accgagatcc tgcctcgcga cctggccgga aaggacatag ccggccgctg cgatccctcc
     1381 gagcaacgcc agcgcattgc cgagaaggtt ggagcccgag aggtcgcctc ctccgatcag
     1441 gccggtgccg aagagggcca acgcgatgcc cacccatgct gcggatctca gccgctcctt
     1501 caagaggaac cacgcggcga tcccggagaa gacgggcgtc gtcgacacga gaagcacgga
     1561 agccgcgatc gatgtcaggc cgatcgacca gatccacgtt gcgaagtgca gagctaggaa
     1621 tacaccggct accgccggca acttcaggtc gactgctcgc agccgcgaga accgcttgcg
     1681 cgcgaacgga gccaagaaag cggcacccgc cgcgcatcgc cagaatgaga tcgccagcgg
     1741 atgcacaccg tccgcatacc gaaccaggat gcccgagacc gaagctgcga ggactcccgc
     1801 aacgaggatc ggccacggtg acagcaggcg ctgctggata gttgcggctc cggccatcga
     1861 gtaaccctag gtgctggggt tcgctttacg ctgccgccat gagcgatggc ccccccaccg
     1921 ttgcggtcgg cgcgatcgtg gtcgacgacg gtgccttgct gatggtgaag cgcgggcacg
     1981 agccggccaa agggctgtgg tcggttcccg gtggtcggct cgaacgcggc gagtacatga
     2041 ccgcagcggt caagagagag gtcaaagaag agaccggcct cgatatcgaa gtcggtggtc
     2101 tggtcgggtt cctcgaggtc ctgggcgacc agcactacgt gatcctcgac ttcgccgcgc
     2161 gtgtcgacgg cgagcggact ccgaacgcgg gggccgacgc cgccgaggcc agatggatcc
     2221 cactagagca gatcgaggaa ctggaatgca cacctcgctt cgtcgagacc ctgaaaagct
     2281 ggggtgtgct gcccgcgtag acggagagct agcagacgaa aagggcgacc aggtcgttct
     2341 ctttcagcat cgtgcccggg atcggatgtg tctccacgac ggtgctacca ccctcacacg
     2401 actcctcgac cctcacccgg aggccgagag agctcaatct cgactgagca gcctcgacgc
     2461 tcatcccgcg gacgtccggc atcctgacct tctggtaacg cgggccggcc gagacctgga
     2521 cctcgatgtg tgagccctcg gagagcatcc ctcccggcgg aggatcggta gccatcacga
     2581 ctccgacctc gatcttgttc
//
LOCUS       DATMED010000253         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3272597, whole genome shotgun sequence.
ACCESSION   DATMED010000253 DATMED010000000
VERSION     DATMED010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272597"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VNP73_06495"
     CDS             <1..243
                     /locus_tag="VNP73_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65607.1"
                     /translation="ERSARYLASGKPVIMQSTGFEDHVPSGKGFLTYTTLDEAVAAVE
                     AVNSDYLSHCEAARSLAEERFDGSRLLTEMLEIAGS"
     gene            240..1211
                     /locus_tag="VNP73_06500"
     CDS             240..1211
                     /locus_tag="VNP73_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWL65608.1"
                     /translation="MTGSASAGIRSELIDELVDLVEKTLEPSRKTKNIDRQTNELSTK
                     MASEFVTIHVDEGSPVRVLVKKVIDDDTFHRPTPPDNELRIYEHLESHPAFAAPRYVG
                     TIGGADPHLVLMAVEGWDLRYQDVDRWVLAARGLGVMHCAFHRDLATLEETGGPPRRS
                     SSEHLAEAKLATAMAQNHHDEAAMILRPLTADYSTIAEELAQHPATLVHGDLAPKNVL
                     IETSMGTERAVFVDWEWGHIGAGTTDLAELVNGLDPEATTRVVDAYVEAARGTALPAD
                     EHAIARCIDLAQLQRTMFRIARSHDWQVPGPQVLEWAEHAASVYERL"
     gene            1217..>2233
                     /locus_tag="VNP73_06505"
     CDS             1217..>2233
                     /locus_tag="VNP73_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65609.1"
                     /translation="MRIIFSAVMSMPPFSPGSALHRLHHILGYQELGHDVYFVEETYT
                     SSCFDERGGATGFEGSVNERTFSRIMRTYGLADRSCLICDGGRETSGMAFDEIVKIAG
                     KADLLINMSGHLSTGPVFDGPACRMYLDQDPVYTQLWQAEYGSDLNFEHHDVFATLGL
                     NIGTDTSPIPDCSIEWIHTLPPVVLNETWSEPASTGNSYTTVASWDVFGDVQFRGEWY
                     GSRRSELVRFAGVPGLVDATFEMLVKSYEDQDEGMIRLLQDNGWSLADAGAITDVDGY
                     LSYIAASRGEICIAKNAYAKARSGWFSDRSANYLAAARPVIAQSTGFESSLPTGVGLL
                     SFSSP"
BASE COUNT          442 a          692 c          678 g          421 t
ORIGIN      
        1 gaacgcagcg ctcgatacct cgcctcgggc aagccggtga tcatgcagtc gaccggtttc
       61 gaagaccacg ttccgtcggg caagggtttc ctcacttaca cgacactcga cgaagcggtc
      121 gccgcagtgg aagcagtgaa ctccgactac ttatctcatt gtgaggcggc caggtccctc
      181 gccgaggaaa ggttcgacgg ctcccgctta ctgacggaga tgctggagat cgccggatca
      241 tgaccgggtc ggctagtgcc gggatccgtt ctgaactcat cgatgagctc gtcgacctcg
      301 tagagaaaac cctggaacct tcccggaaaa cgaagaatat cgatcgccaa acgaacgagc
      361 tctccacgaa gatggcatcg gagttcgtga cgattcatgt cgacgagggc tcgccggtgc
      421 gcgtcttggt gaagaaggtc atcgacgatg acacctttca ccggccgact ccgcccgaca
      481 acgagttacg gatctatgaa cacctggaat ctcaccccgc attcgccgct cctcgctacg
      541 ttggaacgat cgggggggcc gatccgcatc tggtgcttat ggccgtcgaa ggatgggatc
      601 ttcgctatca ggacgtcgat cgatgggtgc ttgcagcgcg cggtctcggc gtcatgcatt
      661 gcgccttcca ccgggacctc gccacgctag aggagactgg tgggcctccg agacgaagca
      721 gcagcgagca tcttgcggag gcgaagctcg ccaccgcgat ggcccagaac caccacgacg
      781 aggccgcgat gatccttcgc cctctaacgg cggattactc gaccatcgcc gaggagctgg
      841 cgcagcaccc cgcaacgctg gtccacggtg acctcgcccc caagaatgtc ctcatcgaga
      901 cgtcgatggg caccgagcgc gccgtgttcg tcgactggga atggggccat atcggagcgg
      961 ggacgaccga cctcgcagag ctggtcaacg ggctcgatcc cgaggcaacc acgcgcgtcg
     1021 tcgacgcata tgtcgaagcc gcgagaggga cggctctgcc ggccgacgaa catgcgatag
     1081 ctcgctgcat cgacctcgcg caattgcaac gcacgatgtt ccgcatcgca cgcagtcacg
     1141 actggcaggt gcccggacca caggtgctcg agtgggccga acacgctgcg agtgtgtacg
     1201 agaggctcta gacgcgatga ggatcatctt ttcggcggtg atgtcgatgc ctccattctc
     1261 ccccgggtcc gcgctgcacc gacttcatca catccttggt tatcaagaac tgggccacga
     1321 cgtttacttc gtagaagaga catatacgag cagctgtttc gacgagcgcg gtggcgcgac
     1381 cggtttcgag ggcagcgtca acgagcgcac cttctctcgg atcatgcgaa cgtacggtct
     1441 cgcagatcgc tcatgcctga tctgcgatgg cggcagggag acctccggca tggctttcga
     1501 cgagatcgtg aagatcgccg gcaaagcgga tctattgatc aacatgtcgg ggcacctgtc
     1561 tacgggacca gtgttcgacg ggccggcctg ccggatgtac ctggaccagg atcccgtcta
     1621 cacgcagttg tggcaggcgg agtacggctc ggacctgaac ttcgagcacc acgacgtctt
     1681 cgctacgctc ggtctgaaca tcggtacgga cacatcaccg atccccgatt gctcgatcga
     1741 gtggatccac acgcttcccc ccgtcgtcct caacgaaacg tggtccgaac ctgcctcgac
     1801 gggcaactca tacacgaccg ttgccagctg ggatgtgttc ggagacgtcc agttccgcgg
     1861 cgagtggtac ggatctcgga gaagtgagct cgttcgcttt gccggcgttc ccggactggt
     1921 cgacgcaacc ttcgagatgt tggtgaagtc ttacgaagac caggacgagg ggatgatccg
     1981 cctgttgcaa gacaatggct ggtcgttggc cgatgccggc gcgatcaccg acgtggacgg
     2041 ctacctgagc tacatcgccg cttcgcgcgg cgagatctgc atcgccaaga acgcatacgc
     2101 caaagcgcgt tcaggctggt tcagtgaccg gtcggccaat tacctggctg cagcccggcc
     2161 ggtgatcgcc caatcgacgg gcttcgagag ctcccttcca actggtgttg gcctcctgtc
     2221 gttctcttca ccc
//
LOCUS       DATMED010000254         4833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1735382, whole genome shotgun sequence.
ACCESSION   DATMED010000254 DATMED010000000
VERSION     DATMED010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735382"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VNP73_06510"
     CDS             complement(<1..427)
                     /locus_tag="VNP73_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65610.1"
                     /translation="MERLTFGFRARRRARRQRILRRAAFASLAAMAIGVPGATAGVEL
                     VERLIGPGPARAAAGIETTESTAGLLRFRNKVVEARPDETLTPTPEGATVAPFTPTTS
                     IEVAIADAAAEFGLDPTYLMSVAECESSMNPGAVSPAGYY"
     gene            646..1662
                     /gene="gmd"
                     /locus_tag="VNP73_06515"
     CDS             646..1662
                     /gene="gmd"
                     /locus_tag="VNP73_06515"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWL65611.1"
                     /translation="MKRALITGVTGQDGSYLSELLLRKGYEVHGLIRRASTFNTGRLE
                     AIYQDPHESGVRFFMHYGDLSDSGSLVNLIRNLEPAEIYHLGAQTHVKVSFEIPEYTV
                     DATAMGTVRMLEAIRASGVETRFYQAGSSEMFGAALPPQSEVTPFHPRSPYGVAKVAA
                     HWATINYREAYGMFATNGILFNHESPRRGETFVSRKITRAVARIKAGIQDKLFLGNLD
                     SKRDWGYAPEYVEAMWTMQQHDEPLDLVIATGESHSVREFAERAFAHADLEWEKHVEV
                     DPNYFRPAEVNFLQGDASRAEGTIGWEPKVRFEELVRIMVDGDIKLLEDEMSGRLVRQ
                     DRDH"
     gene            1683..2843
                     /locus_tag="VNP73_06520"
     CDS             1683..2843
                     /locus_tag="VNP73_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWL65612.1"
                     /translation="MELEIGIGKSGRRAYGFDDIAIVPSRRTRDPDDVDISWEVDAFK
                     FELPLMAAAMDGVVSPSAAIEVGRLGGLAVLNLEGLQTRYDDAEAVLSEIAELDTDKA
                     THRMQELYQEPIKEELIYKRIREIKDAGHHAAASLTPQRVERFHKIALEAELDILVIQ
                     GTVVSAEHVSTRTEPLNLKRFIATYEIPVIVGGCASYSTALHLMRTGAIGVLVGVGPG
                     AACTTRGVLGVGVPQATAIADAAGARIRHLDETGRYCHVIADGGMRTGGDIAKAIACG
                     ADAVMIGSPLATAKEAPGRGYHWGMATFHPTLPRGARVKSGTLGSLEEILVGPAHEND
                     GRLNLFGALRTSLATCGYANIKEFQKAEVMVAPALKTEGKDLQRSQNIGMGR"
     gene            2988..3116
                     /locus_tag="VNP73_06525"
     CDS             2988..3116
                     /locus_tag="VNP73_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65613.1"
                     /translation="MLEGLAVVGAVVSSLAAFEVLASRYGRDSRDGNDWFVHRADH"
     gene            3129..3632
                     /locus_tag="VNP73_06530"
     CDS             3129..3632
                     /locus_tag="VNP73_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWL65614.1"
                     /translation="MALALDRIDRQILKLLREDGRISHAAIAKEVGLSGPAVHERVRK
                     LEQNGAIAGYAAILDPQVLDRSHVAFVRVNLSEGAEFVADEPVVARICDEPDVLEFHR
                     IAGEDCYLIKARTATNKDLEQLLRRIRSLPGVAKTRTTIVLSTELEKPSIDPSSQGAS
                     PAEISPG"
     gene            4072..4833
                     /locus_tag="VNP73_06535"
     CDS             4072..4833
                     /locus_tag="VNP73_06535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL65615.1"
                     /translation="MKDHDRLETEFGTYAEWLAQAIASLSFEDPIPAACRGTGNPALF
                     ERLADLLEAGPDVTVLDVGCGIGGPGAWLMRERGCRVVGVDLMQPSLRGLKLLFPEFR
                     TVRASTGALPFTTDAFERAWMLGVLEIVADKRRALAELQRVIQPGGRVVIYTFVKTGD
                     DLDEVPEADVFERAEFVTGCASDVGFGVRSAQPVTDIPKIPDGWRDVTSRVRGRLWEL
                     HANDPLLERVELDLARISRLTRSREVEPWEFVLEK"
BASE COUNT          922 a         1491 c         1561 g          859 t
ORIGIN      
        1 cgtagtaacc ggccgggctc acggccccag ggttcatgct cgactcgcat tcggcgacgc
       61 tcatcaggta ggtgggatcg agcccaaact ctgcggccgc gtcggcgatc gccacttcga
      121 tggacgtcgt gggggtgaag ggtgccaccg tggccccctc gggggtcggc gtaagtgtct
      181 cgtcgggccg ggcttcgacg accttgttcc tgaagcggag caagcccgcc gtcgattcgg
      241 tcgtctcgat gccggccgcg gcgcgggccg ggccgggccc gatcaagcgc tcgaccagct
      301 cgacacccgc ggtcgccccc gggacgccga tggccatcgc cgccagggac gcgaacgccg
      361 cccggcgcag gatccgctgg cggcgcgccc tgcggcgcgc gcggaatcca aaggtaagtc
      421 tttccaccgt gctcgcctcc aattggaagg gggctaccga gcgccctccc ttggtccgcc
      481 gaccggaaag tcctacccct attttaacag ttgcaagcat ttgtgaaatg gcgcacaaac
      541 tcctttaata ggtccctcta gctggggaaa tggaggggac ggccgcctgg gcccgctatt
      601 tcgagcccgc cagtggggga ccgtaccacg tcttatgctt gctatatgaa gcgcgccctg
      661 atcaccgggg tgacaggcca agacggctcc tacctatccg agctcctact gcggaaggga
      721 tacgaggtcc acggcctgat ccgccgggcc tccaccttca ataccggccg cctggaggcg
      781 atctaccagg acccccacga gtccggggtg cggttcttca tgcattacgg agatctctcc
      841 gactcgggct cgctcgtgaa tctgatccgc aacctcgagc cggccgagat ctaccacctc
      901 ggggcccaga cccacgtgaa ggtttcgttc gagatcccgg agtacaccgt ggacgccacc
      961 gccatgggta cggtgcggat gttggaagcg atccgagctt cgggcgtcga gacccgcttc
     1021 tatcaggccg ggtcgtcgga gatgttcgga gcagcccttc ctccccagag cgaggtcacg
     1081 ccgttccacc cgcgcagccc atatggggtt gcgaaggtgg ccgctcattg ggccacgatc
     1141 aactatcgcg aggcttacgg gatgtttgcc acgaacggga tcctgttcaa ccatgaaagt
     1201 cccagacgcg gtgagacgtt cgtcagtcgc aagatcacgc gcgccgttgc gcgcatcaag
     1261 gcgggcatcc aggacaagct cttcctgggg aacctcgact cgaaacggga ctggggttac
     1321 gcccccgaat acgtcgaagc gatgtggacg atgcaacagc acgacgagcc gctcgatctg
     1381 gtgatcgcta ccggcgagag ccactcggtg cgggagttcg cagagcgagc tttcgctcat
     1441 gccgatctcg agtgggagaa acatgtcgag gtcgatccca actacttccg cccggccgag
     1501 gtgaacttcc tgcagggaga cgcctctcga gccgagggga ccatagggtg ggagccgaag
     1561 gtccggttcg aagaactcgt tcggatcatg gtggatggag acatcaagtt gctcgaagac
     1621 gagatgagtg gccggctggt ccggcaagac cgcgatcatt agggaaggag cgtggatcgg
     1681 tagtggaact cgagatcggt atcggcaagt cgggccgtag ggcctacgga ttcgacgaca
     1741 tagcgattgt gccctcccgc aggacccggg accccgacga cgtcgacatc tcgtgggagg
     1801 tcgacgcgtt caagttcgag cttcccctga tggctgccgc gatggacggt gtcgtctcgc
     1861 cgtcggcggc gatcgaggtc ggtcggctgg gtggactcgc cgtgttgaac ctcgaaggac
     1921 tccaaacgag atacgacgat gccgaggccg tcctctccga gatcgcagag ctggacaccg
     1981 acaaggcgac gcaccggatg caagagctct accaggagcc aatcaaagag gaactgatct
     2041 acaagcgcat ccgcgagatc aaggatgcgg gccatcacgc ggctgcgtcg ttgacgccgc
     2101 agcgcgtcga acgtttccac aagatcgctc tagaggccga gctcgacatc ctcgttatcc
     2161 agggaacggt cgtgtcggca gagcacgtct ctacgcgaac ggaaccgctc aacctgaagc
     2221 gattcatcgc gacctatgag atcccggtca tcgtgggtgg ttgtgcttct tattccacgg
     2281 ctctgcactt gatgcggacc ggagctatcg gcgtcctcgt gggcgtggga ccgggtgcgg
     2341 cgtgcaccac gcgtggtgtt ctcggagttg gtgtgccgca ggcaaccgcg atcgccgatg
     2401 cggccggagc gcgcatccgg cacctcgacg aaaccggtcg ttactgccac gtcatcgccg
     2461 acggtgggat gcgaaccggt ggcgacatcg ctaaggcgat cgcatgtggc gcggacgcgg
     2521 tgatgatcgg ctcgcctctg gcgactgcca aagaggcccc gggtcgtggc tatcactggg
     2581 ggatggcgac gttccatccc acccttccga ggggagctcg ggtgaagagt gggacgctcg
     2641 ggtcgctcga ggagatcctc gtcggcccgg cgcacgagaa cgacggccgg ctcaatctct
     2701 tcggggcctt gaggaccagc ctcgccacct gcggctacgc caacatcaag gagttccaga
     2761 aggccgaggt gatggtcgct ccggccctca aaaccgaagg gaaggacctc cagaggagcc
     2821 aaaacatagg catggggcgg tagtccgcca agtaatccaa ggttggatcc aggaggcgcc
     2881 taggaaatct aggcgtcttc tttatttggc ctaaagttcc ttgacctccg taccgatact
     2941 tggtaaggta cgtaacaagg atagaccctt acagggagga ggacaagatg ctggaaggac
     3001 tcgcagttgt cggagcggtg gttagctcgc ttgcggcctt cgaggtcctg gcctcccgct
     3061 acgggcggga tagccgggac gggaacgact ggttcgttca ccgagccgat cactaaggtc
     3121 gggacgcagt ggcacttgct ctcgaccgca tcgaccggca gatcctgaag ctccttcgcg
     3181 aggacggccg catcagtcac gccgccatcg cgaaggaggt cgggctgtcg ggccccgccg
     3241 tccacgaacg ggtacgcaag ctggaacaga acggcgcgat cgccggctac gccgcgatcc
     3301 ttgaccctca ggtactcgac cgctcccacg tggcgttcgt ccgcgtgaac ctgagtgagg
     3361 gggccgaatt cgtagccgac gagccggtgg tggcgcggat ctgtgacgaa cccgacgtgc
     3421 tcgaattcca ccggatcgcc ggcgaagact gctacttgat caaggcacga accgcgacga
     3481 acaaggatct cgagcagctt ctccgccgga tccgatcgtt acccggcgtc gccaagaccc
     3541 gcaccacgat cgtgctctct accgaactcg agaagccgtc gatcgatccc tcatcacaag
     3601 gggcctcgcc ggccgaaatc agccccggct aggtgttgtc agtcgtcact tcggaccaat
     3661 tcccggaacg ggggagcctc cagcgtcttg tttcgcggcg gaccacacgt ccgccactcg
     3721 ctcacgcatc acacaagatc aacttcgttt ccgggttgcc acgataaatc gaacgatcgc
     3781 accagcgcat gcgattacaa gacttagtgc caggttggcc tcgcctgcaa ctagccaggt
     3841 caggcccaag tgagtcgcga agaaagcgat gccccaaagc acagcctgtc ggacgggcgt
     3901 cacaaaactt tccaccgtct caataatcac actctttgag gctcccatga tcatctgaca
     3961 agctgccgat tccggaaccc agcatgcaac ccggccgcta gcaatgcgag acggcacagt
     4021 cgctgagtgc tcaaccagcg atatcccggc ggcctcatag gctcgccggg cgtgaaggac
     4081 cacgaccgtc tcgagaccga gttcggcact tatgccgagt ggctggcaca ggcgatagcc
     4141 agcctttctt tcgaggaccc catcccggct gcctgtcggg ggaccgggaa ccccgctctg
     4201 ttcgagcggt tggcagatct gctcgaagcg ggaccggatg tcacggtgct cgacgtaggt
     4261 tgcggcatcg ggggcccggg cgcgtggctg atgagggagc gtgggtgccg cgttgtcggc
     4321 gtcgacctca tgcagccttc tctccgtggg ctgaagctct tgttccccga gttccgaacg
     4381 gttcgggcgt ccaccggtgc actcccgttc acaacggatg ctttcgagcg tgcatggatg
     4441 ctcggagtgc tggagatcgt cgcagacaaa cgccgcgcgc tcgccgagtt gcaacgggtg
     4501 atccaacctg ggggccgagt ggttatctac accttcgtga agacggggga cgacctcgat
     4561 gaggttcccg aggcggacgt cttcgaacga gccgagttcg tgaccggatg tgcgtctgac
     4621 gttggttttg gcgtgaggag cgcgcagccc gtaacggaca tacccaagat cccggacggg
     4681 tggcgcgacg taacctcccg ggtacgcggg cggctgtggg agctgcacgc taacgatcca
     4741 ttgctggagc gcgtggaact cgacctagcg cgcatcagtc ggctcacgcg atcgagagag
     4801 gtcgagccgt gggagttcgt tttggagaaa tag
//
LOCUS       DATMED010000255         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2511834, whole genome shotgun sequence.
ACCESSION   DATMED010000255 DATMED010000000
VERSION     DATMED010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511834"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(425..1819)
                     /gene="sthA"
                     /locus_tag="VNP73_06540"
     CDS             complement(425..1819)
                     /gene="sthA"
                     /locus_tag="VNP73_06540"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HWL65616.1"
                     /translation="MSYDYDLLVIGSGPAGQKAAIQAAKLGRRVALVERRELGGVSVN
                     WGTIPSKTLREAIVYLTGLSQRATYGQSYRVKEEITVEDLRLRTQGVIDREIDVVRDQ
                     LMRNHVQIVHGSARFLDAHTVAVSGNEERQVAADRVVIATGTRPAHPPDVDFDDRTIL
                     DSDGLLGLTRIPHSVVVVGAGVIGIEYASMFAALGAKVTVVEKRARLLDFCDAQIAEG
                     LQYHLRDVGVVFRLGEEVTAVERHADGALTQLASGKQVASEVVLYAAGRQGETEDLEL
                     ERAGLEADERGRIVVGPDYRTAVEHIFAAGDVLGWPALAATSMEQGRLASAHAFGEDA
                     SMSELLPIGIYTIPEISYVGRTEEELTEAAVPYEVGISRYRELARGHILGDTHGLLKL
                     LVSREDKTILGVHVLGTNATEVIHIGQAVMGLNGTIDYLVDAVFNYPTLAESYKVAAL
                     DARNRLMAVERIVR"
BASE COUNT          349 a          673 c          626 g          369 t
ORIGIN      
        1 gctaaccccc gtaaggcagc cggcgaaaat ccctgctcgt gacgggaatc cccgactggt
       61 ccgggtcagg cccggaaggc cgtatggggc tttgcaagcc ggaggtcgcc ggttcgatcc
      121 cggcacgctc catgaaatcc ctgcaaatag gcctttttct gttgtcgcga cagacggaag
      181 tggggttgca agcgtcgatc cttggtacgc gttgagccgg tgaaagccgc tcgcagccgc
      241 aaatacccgc tggctgagct gccggtcgaa gaggccggag ttcaggttgg tgtcgtgcgt
      301 agccgtggct ttttgcaggg attcgttcga tgtgcgcgcg tggtcgctgg gctccatccc
      361 gcgcacatcc cgcacgcaac ccggctaatg cggagcagtg gagggttctc tgtccccgcc
      421 gtgactaacg gacgatgcgc tcgacggcca tgagccggtt ccgcgcatcc aaggccgcaa
      481 ccttgtacga ctcggccagc gtggggtagt tgaaaacagc gtcgaccaga tagtcgatcg
      541 tcccgttgag ccccatcacc gcctgtccga tgtgaatgac ctcggtggcg ttcgtgccga
      601 gcacgtggac gccaaggatc gtcttgtcct cccgcgagac gaggagcttc agtagcccat
      661 gcgtgtcgcc caagatgtgc ccgcgcgcaa gctcgcgata ccgagagatg ccgacctcgt
      721 acggcaccgc tgcttccgtc agttcctctt ccgtgcggcc gacgtagctg atctcgggga
      781 tcgtgtaaat cccgatcggg agcaactcgc tcatcgaagc gtcctcaccg aaggcgtgtg
      841 cggaggcaag gcgcccctgc tccatcgaag tggctgcgag cgccggccaa ccgaggacgt
      901 cgcccgcagc gaagatgtgc tcgacggcgg tccggtagtc cggaccgacg acgatccgcc
      961 cgcgctcgtc ggcctccagg ccagctcgct cgagctcgag atcctccgtt tccccctggc
     1021 gaccggcggc gtagaggacg acctcggagg cgacctgctt cccgctcgcg agctgcgtca
     1081 gcgcgccatc ggcatgacgc tcaacggcgg tcacttcctc acccaaccga aagacgacac
     1141 cgacatcgcg caggtggtac tgcagaccct cggcgatctg ggcatcgcag aagtcgagca
     1201 ggcgcgcgcg tttctcaacg accgtgacct tcgcgccgag cgctgcgaac atggaggcgt
     1261 actcgatgcc gatcaccccc gcccctacga cgaccacaga gtgcgggatt cgggtcagtc
     1321 ccagcaggcc gtcggaatcg aggatcgttc ggtcgtcgaa gtccacgtcg ggcgggtggg
     1381 ctgggcgtgt ccccgtggca atgacgactc tgtccgcagc tacctgccgc tcttcgttac
     1441 ctgacacagc aacggtgtgc gcgtccaaga aacgagcgct cccgtgaacg atctggacgt
     1501 ggtttcgcat caactggtcg cggacgacgt cgatctcgcg gtcgatcacg ccctgcgttc
     1561 gcaggcggag atcttctacc gtgatctcct ccttcacgcg gtagctctgc ccgtacgtcg
     1621 ccctctggct caacccggtg aggtaaacga tcgcctcgcg gagcgtcttt gatgggatcg
     1681 tcccccagtt gaccgacacg ccgccgagct ctcgtcgctc gacgagagcg acgcgccggc
     1741 cgagctttgc cgcctgaatc gccgccttct gtccggcggg cccagatccg ataacgagaa
     1801 ggtcgtagtc gtaactcatc gttagctccg cgcgagtctt ccgatcaaga gtgcgatcga
     1861 gctcccaggt ctcggaacac cgtcgcttcg agcaccgccc ggccgctcca tagtctcagc
     1921 acctcctcga cctggggaca ctctacggcg agcgtagacc caccaaccga gtgcaaggcg
     1981 gacgatttct cgggccttca ggtagggctt ttcgaac
//
LOCUS       DATMED010000256         4185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5563913, whole genome shotgun sequence.
ACCESSION   DATMED010000256 DATMED010000000
VERSION     DATMED010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5563913"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VNP73_06545"
     CDS             complement(<1..569)
                     /locus_tag="VNP73_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL65617.1"
                     /translation="MTQPTDGGVAAYVLRLLRSQLESGWVVSVASPAGEFSTEVVGAG
                     GRWLEWDAGRSPGPMVPAETRALAAVAGRAGADVIHLHSSKAGLAGRLNRRMAPRIFQ
                     PHGWSWDAVGPTAGRVVRAWERFAAGRCDLLICVSEEERTRGEAAGLNAPWCVIENAV
                     DLERFPRCDDSDRAAAREELGLPSGPLAV"
     gene            607..2460
                     /locus_tag="VNP73_06550"
     CDS             607..2460
                     /locus_tag="VNP73_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HWL65618.1"
                     /translation="MKRRASIAGISLILSLGLAAAIPLARAGLNLLKARASLTADAAS
                     LDRRDVVEAKTRLDSASDSFASLPLRVLAALPGIGDNYDSMRAVTEASADVMEKALVL
                     QDWREDNVSGIFAGGRVDLDLLAGVREPLAAQTEALEELELAAQESRSGWLLPPVWDA
                     LEELGTRAGELRSGSEKAQRLLDHAGALFGDPEPRTYLVMSMNNAELRGAGGLLSGIG
                     TLTMDHGRIDVGRQWPREYFQRKPYRSVPASRAYLERYGNFKANTTLWANVTFSPDVV
                     EDARIASQLFELQTGTKTDGALVVDPRGLEALVPADAAIEVPGSDRVIEADELATWIY
                     SDAYAEFADDDARRLAILKVGRLALSAALEGEPQSGMLESLGEAVAGKHVSFVSFEKD
                     ELSALEDLGLAPTLGEAPGDHALVAVHNFGDGGNEGTKLDYWTDRTVRHACSIGEGSL
                     DPCVLELTLLNSAPTGLTRYVAGAPYAMLRSYVELFIPAKATVVGVYLDGEPAEFATF
                     DEGDVQSVSVYTQVPRSEQRTITIHYGLPPRNDYSLLFSPQPLARDARIDLGLRVPAD
                     WTVESPLTRSGDVYRYAGTLEGDVRVTARPDARTGIPALWDAFVDLLRSSL"
     gene            complement(2457..3089)
                     /locus_tag="VNP73_06555"
     CDS             complement(2457..3089)
                     /locus_tag="VNP73_06555"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWL65619.1"
                     /translation="MPFSPEEIEHKDFLLTLRGYDKEEVRAFLEAVASDYRALTSAAR
                     DRDDVPDTAYEQIGREVGTVIQVAKESATAMKRQAEAEASEMRTRADREAQKIREAAT
                     NAAKRLHDEADKYSIEARSQADLEASAKIRDSHKRLERLQSTESKVRQRLYSLEAMIA
                     NLRRELETSDELGAGELDLSTDNGSKDETEPEVTATVAADSAVEESKTLS"
     gene            complement(3253..3687)
                     /locus_tag="VNP73_06560"
     CDS             complement(3253..3687)
                     /locus_tag="VNP73_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65620.1"
                     /translation="MTSKAIRIVIAVAFLLLLGSITMAAPALAQREPYSNGNCCNDND
                     DDDDDDDDSPPITSEELPSFPTPANTPPPEVLGGQIPGAVPRPETQPDVQPGIVVPEA
                     QQPGILPFTGADLLLYGMTGAAAIGTGMSLVRIGRRSKPDDE"
     gene            complement(3684..>4185)
                     /locus_tag="VNP73_06565"
     CDS             complement(3684..>4185)
                     /locus_tag="VNP73_06565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWL65621.1"
                     /translation="IALAIYFDSGRPILLRQLRVGLDGQPFNMLKFRTMHTSEAEVEA
                     TEWSADEDRITKVGRVLRDTSLDELPQLVNVVRGEMSLVGPRPERPHFVDMFSERYPN
                     YAERHRLPAGVTGWSQIHGLRGDTSIEERAAFDNYYVDNWSLSQDIKILLRTVTAFKN
                     TPGGSI"
BASE COUNT          736 a         1383 c         1278 g          788 t
ORIGIN      
        1 cagaccgcga gcgggcccga aggaagcccg agctcctctc ttgctgcagc ccgatccgaa
       61 tcgtcacaac gagggaagcg ctcgaggtcg accgcgttct cgatcacgca ccagggcgca
      121 ttcaggcccg ccgcctctcc cctcgtcctt tcctcttcgc tcacgcagat caacagatcg
      181 cagcggccgg ctgcgaacct ttcccacgcc cgcaccaccc gcccggcggt tggtccaacc
      241 gcatcccacg accatccatg tggttggaag atgcgggggg ccatccggcg gttcaatcgc
      301 cccgcaagac cggccttgga tgaatggaga tggataacgt cggctccggc tcgtcctgca
      361 actgctgcta aggcccgggt ctcggccggc accatcggac ccggcgaccg acccgcatcc
      421 cattccagcc atcgccctcc ggcccccacg acctcagtgg agaactcccc ggccggagag
      481 gcaaccgaga ccacccatcc cgattcgagc tgcgatcgca gcaatcggag cacgtaggcg
      541 gccacccctc cgtcggtcgg ttgggtcacg tgaagcacgt gatgagacgg gcttagggtt
      601 tggcgcttga aacgaagagc ttccattgcg ggcatatccc tcattcttag cctaggtctc
      661 gccgcggcga ttccactcgc gcgagcgggc ctcaatctgc ttaaggcacg tgcctccctg
      721 accgccgatg cagcgagcct cgaccggcgc gatgtcgtcg aggccaagac ccggctcgat
      781 tcagcatcgg attcgtttgc atccctccca ctgcgcgtcc tcgcggcgct cccgggcata
      841 ggagacaact acgactcgat gcgagcggtg acggaggcct ccgccgacgt gatggagaaa
      901 gccctcgtgc tccaggactg gcgcgaggac aacgtctcgg ggatcttcgc cggaggccgt
      961 gtcgacctgg atctcctcgc cggcgtgcga gagccactcg ccgctcagac cgaggcccta
     1021 gaagagctcg aactagcggc ccaagagagc cgctcggggt ggcttctgcc gccggtgtgg
     1081 gacgccctgg aagagctcgg cacgcgtgcc ggggagctgc gctccggatc cgagaaggca
     1141 cagcggctgc tcgatcacgc cggtgcactc ttcggtgacc ccgaaccgcg cacctacctc
     1201 gtgatgtcca tgaacaacgc cgagctgcgg ggggccgggg gcttgctctc aggtatcggg
     1261 acgctgacga tggatcacgg ccggatcgac gtcggtcgcc agtggccgcg cgagtacttc
     1321 cagcgaaagc cctaccgcag cgtccctgca tcccgcgcat acctcgagcg ttatgggaac
     1381 ttcaaggcga acacgacgct ttgggcgaac gtcaccttct cgccggacgt cgttgaggac
     1441 gcgcggatcg cctctcagct cttcgagtta cagaccggga ccaagaccga tggtgcactc
     1501 gtcgtcgacc cccgagggct cgaggcactt gttcctgccg acgctgccat cgaggtgccg
     1561 ggttccgacc gggtgatcga ggcagacgaa ctggccacgt ggatctattc cgacgcatac
     1621 gccgagttcg ccgacgatga cgccagacga ctcgccatcc tcaaggtcgg ccggctggcc
     1681 ctatccgcgg cgctggaagg cgaaccccag tcggggatgc ttgagtctct cggggaagcg
     1741 gtcgccggaa agcacgtctc gttcgtctcc ttcgagaagg acgagctgag cgctctcgaa
     1801 gatctggggc tcgccccaac actcggggag gcgcccggag atcacgctct cgtcgcggtt
     1861 cacaacttcg gcgacggcgg caacgagggg acgaagctcg attactggac ggatcgaacg
     1921 gtgcgacacg cttgttcgat cggagagggt tcgctcgatc cctgtgtgct cgagctgact
     1981 cttctcaaca gcgctcccac gggactgaca cgttacgtcg caggggcgcc gtacgccatg
     2041 ttgcgcagtt atgtcgagtt gttcatcccg gcgaaagcga ccgtggtcgg cgtctacctc
     2101 gacggcgaac cggccgagtt cgcgacattt gatgagggcg acgtgcaatc cgtgtccgtt
     2161 tatacccagg tgccgcgctc cgagcagcgc acgataacca ttcattacgg cttacccccc
     2221 cgtaacgact actcgctgct gttcagcccg caaccgctcg cgcgcgacgc gcggatcgac
     2281 cttggcctcc gagtgcctgc ggattggacg gtcgaatcac cattgacacg aagcggagat
     2341 gtgtatcgct acgccggcac gcttgaagga gatgtgcgcg tcaccgcacg tccggacgca
     2401 cgcacgggga taccggcgtt gtgggacgcg ttcgtcgacc tattgagaag tagcctctag
     2461 ctgagggtct tagattcctc cacggcggag tcggcggcca ccgttgcggt cacttccggt
     2521 tcggtctcgt ctttggaacc gttgtcggtg ctgaggtcga gctcgccggc ccccaactcg
     2581 tcgctggtct ccagctcgcg acgaaggttc gcgatcatcg cttcgagcga gtacagccgt
     2641 tgcctgacct tcgactcggt cgactgcagc ctctccagcc gcttgtggct atcgcgtatc
     2701 ttggccgacg cctcgaggtc tgcttgcgaa cgagcctcga tcgagtactt gtcggcttcg
     2761 tcgtgcagcc tcttcgcggc gttggtcgcg gcctccctga tcttctgagc ctcacggtct
     2821 gcacgcgtcc gcatctcgct cgcttcggcc tcagcctgac gcttcatcgc cgtggccgat
     2881 tccttcgcga cctggatgac cgttcctact tcgcgcccga tctgctcgta cgcggtgtcg
     2941 ggaacatcgt ctcggtctcg cgcagccgag gtgagcgccc gatagtccga agcgacggct
     3001 tccaagaagg ctcgtacttc ttccttgtcg taaccgcgca gtgtcagcag gaaatctttg
     3061 tgctcgatct cctcgggtga aaagggcact caggccaccc cctgttctcg gcggcgcctc
     3121 ttcgagcgag cgccgttcaa cggatcctca acgattcttt aaccgcgagc ctagttcctg
     3181 caactggacg gcctgacagg gcggccctac aggccttcgg ctcggtaggg acagcagcta
     3241 gaaatgcgcc gactactcgt cgtcgggctt ggacctacgc ccgatcctca cgagcgacat
     3301 ccccgtaccg atggctgcgg ctcccgtcat gccgtaaagc aggaggtctg cacccgtgaa
     3361 gggcaggatg ccgggctgct gagcctcggg aaccacgatg ccgggctgca cgtcgggctg
     3421 cgtctcggga cggggcacgg caccagggat ctgacccccg agcacctcgg ggggtggagt
     3481 gttggcgggg gtggggaagc tcggcaactc ctcgctcgtg atgggcggcg agtcatcgtc
     3541 atcgtcgtcg tcgtcatcgt tgtcgttgca acagtttccg ttcgagtagg gctcgcgttg
     3601 cgccagagca ggcgccgcca tagtgatcga gcccaacaac aacaggaacg caaccgcaat
     3661 aacgatcctt atcgctttac ttgtcatatc gaacctcccg gggtgttctt gaatgccgta
     3721 accgtcctca acaagatctt tatgtcttgg gacaacgacc agttgtccac gtaatagttg
     3781 tcgaaagccg cccgctcctc gatcgaggta tctccgcgta gcccgtgaat ctgtgaccat
     3841 ccggtaacgc cggcgggaag tcgatggcgt tcagcgtagt tcggatagcg ctcagagaac
     3901 atatcaacga aatgcggccg ctcgggtcgc ggcccgacaa gcgacatctc gcccctcaca
     3961 acgttcacaa gctgaggaag ctcatcgagg ctggtgtcgc gtagaacgcg gcccaccttt
     4021 gtgatgcgat cctcatcggc ggaccactcg gtcgcttcga cctctgcctc cgacgtatgc
     4081 atcgttctga acttcagcat gttgaagggc tgaccatcca atccgacgcg caactgccgc
     4141 aacaggatgg gccgacccga atcgaagtag atcgccaggg cgatc
//
LOCUS       DATMED010000257         8716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5195355, whole genome shotgun sequence.
ACCESSION   DATMED010000257 DATMED010000000
VERSION     DATMED010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5195355"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VNP73_06570"
     CDS             <1..1180
                     /locus_tag="VNP73_06570"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HWL65622.1"
                     /translation="ASLLSLHSPALARAAWKERIDGIVRGRAIGVAVREAGTTLYRHA
                     ATRRRVPASNQKLLLTMALLDRVGAEFSFTTSAAAAESPEDGVVDGDLWLLGRGDPTL
                     SSERRYTRSLPVDTTKIKSLAGAVREAGITRIRGSVIGSTSYFARDWFAIGWKAGYPR
                     DECPLPSALAINGNVNDGGNHVSDPEIRAARSLSQRLEKMGIRVSGPPGAAEEPPGLE
                     EIASVLSEPLPSILQHLNVNSSNFFAEMLGKRLAVEARGRPGTIAKGAGAVEGWADRN
                     GVTVIAHDASGLSYQNRVSPLGMVRLLGRVEHEEWIDPFRGTLARADEGTLENRLNGV
                     RVRAKTGTLDKVSTLSGYVWLRSRDVWGEFSIMSSGMEKTTAADIENRIVRILTRSAG
                     "
     gene            complement(1323..1835)
                     /locus_tag="VNP73_06575"
     CDS             complement(1323..1835)
                     /locus_tag="VNP73_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65623.1"
                     /translation="MREKIMAAVISISVAGFIGATPAVAGDHTEKTHRGHGKRAMGTI
                     ESFDGTTLSVALDDTTTWSGPVACDVKVRVEHRGRHARGKGHGNPSRGSVTDLKPGAW
                     ILRMKLDDGIVEKVHVRPAPTDGEMVTAASGASDDCDSEEEESDEAGTDTLRDENAKE
                     EEDAAEGDEG"
     regulatory      complement(1847..1921)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2064..2294)
                     /locus_tag="VNP73_06580"
     CDS             complement(2064..2294)
                     /locus_tag="VNP73_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HWL65624.1"
                     /translation="MGIGASLFLIALGAILAFAVSFDAEGFDINTIGIILLIVGVLGG
                     VLSMIFWSSWGGWGNRRDTVVHTDRVREREVV"
     gene            complement(2365..3927)
                     /locus_tag="VNP73_06585"
     CDS             complement(2365..3927)
                     /locus_tag="VNP73_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWL65625.1"
                     /translation="MTNFDAIVIGAGHNGLVCASYLQRAGLKVVVLERTDRIGGACVT
                     EELFPGFRISTASYSLSLLLPQIVSELGLELDVRIKEPKVFAPFPDGGGLFLWRDEKR
                     THDEISKVSKKDADAYLPFEELFEEAALRLRPLLTYPATRKQARRAFRQSEVERLFAR
                     TVDGSISDLCEEYFESDLMQGLVASQGIIGSAAGPKTPGTAYIYLHHAFGEATGESGV
                     WGLVRGGMGAITTELADRFRAAGGEIRLEAEVASVRLDDRRRASGVDLVSGEELSAPI
                     VCSNADPVRTVGFLRDAHLPPEYLEDVGLMPTKGTVVKVNCALSGLPRFKGMEGGNEP
                     GPEHTGTITISPSIDYLEEGARAAGVGEPAEKMFIEAWMQSASEPDLAPPGKHTLSIF
                     AQYAPYDLAEGDWSTRRDEIGDIVLRTLEEYAPGLTDLVEDRLVLGPPDLEERFGLTG
                     GNIFHGELLPDWLFEKRPATAWHRYRAPMAGLYLCGSGAHPGGGVCGAPGRNAARAVL
                     SDLTAKGDRANT"
     gene            3984..4145
                     /locus_tag="VNP73_06590"
     CDS             3984..4145
                     /locus_tag="VNP73_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65626.1"
                     /translation="MAISAFCTTCQRTVYVEEGDTPVCPVCSSPLLETVESSSEDDAV
                     ATDQSPGAK"
     gene            complement(4124..4720)
                     /locus_tag="VNP73_06595"
     CDS             complement(4124..4720)
                     /locus_tag="VNP73_06595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWL65627.1"
                     /translation="MNATIHLVRHGRVENPKGIIYGRLPGFHLSDRGLRQAEGAAQHL
                     GDRDVGAIWSSPLERAQETARAIAAHHDVEIVTDDRLIESDSTLEGVGRSIATFARSP
                     RVWWSFRNPWRPSWGESFAEIRVRMLDAIDEAVRSSGGRELVVVSHQTPVIVARLSLT
                     KSRVPPWMGFTPCSTGSVSSVVIEGGDAVSVSYFAPGD"
     gene            4747..5823
                     /locus_tag="VNP73_06600"
     CDS             4747..5823
                     /locus_tag="VNP73_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWL65628.1"
                     /translation="MPEVLLFGATGYTGRLTAQALHRRGANFAISGRDHGKLEALATT
                     TGNPEIRVASVGDVRGLAAALEDVKVLITCVGPFEQLGSTAVEAALQAGVHYIDSTGE
                     GPFIARLIENKMEQARKAGIAMAPAMGFDEVPADVAATLASRDLDPVDLTLTYAVPLT
                     PSAGTLRSALGILSEPAAWIENGRRVTVKAGQRSRWAPMPPPLGPRSSVAFPLAEGHL
                     APLHLELDSLRVYATVGMAQRLGLKLGGPFLPLLKSGPIKSVTESLIGRLPEGPTDQQ
                     RRAGQWTILAEARSGDAWRNVALTGTDVYGLTAEFLSAAALRFAGDGPLETGVVAPVQ
                     AVGLEVWEKEFADHGVTIQTFEPH"
     gene            5840..6235
                     /locus_tag="VNP73_06605"
     CDS             5840..6235
                     /locus_tag="VNP73_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65629.1"
                     /translation="MGARLAYAKVIDRQVFYEKGGNLHPKLENEVLVQAEPGKAGAFL
                     VFRAWADDHGAFTEQWAIKEPEGRTIYQSSPREIHMPTPSHTERLEDEVADLVLDFAA
                     DDYQLVFFLDEREIARMNFPVKVSSNGNG"
     gene            6427..6804
                     /locus_tag="VNP73_06610"
     CDS             6427..6804
                     /locus_tag="VNP73_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65630.1"
                     /translation="MKTWLIATGAIYALGAVDFIARPWAATASLGNVGGDEMETESVG
                     LYNALASSYMATIAALSFSAARDPLDKRDLIPPLLVAKAVSSATMMYRYFTTKKRGYA
                     YGAALDAFLLGTTAGLAASLEEG"
     gene            6919..7227
                     /locus_tag="VNP73_06615"
     CDS             6919..7227
                     /locus_tag="VNP73_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65631.1"
                     /translation="MYEFAIVVLLGIGTFKVIDMLNEYVDLAKVQSILTIALGAVVCW
                     ALGFSLFEHWGMAVRSEAMGYLGTGLMVSAAGYATPKVFEHLAEVIGHPRSHTHSRAA
                     "
     gene            7322..8593
                     /locus_tag="VNP73_06620"
     CDS             7322..8593
                     /locus_tag="VNP73_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65632.1"
                     /translation="MNDPIYLRRLESRVTLVRAAFCAGLLAAWATGSLPTQETSGAVL
                     RALGPVLALGTLAIAFAARGLRGDRARTRLGVTAFLFDVSIAAVAALAAGEQIAIAFV
                     LVMIPIEAALRYGLRPSIVVAIGVSLLFGAVWWDSPATIALVGGVCLGTAWVVGSAVQ
                     IWVEKQRFLERQTAQLIEHDRLRDRNVAITSHEIRSPLTAVIAATDVLKRKGDEIEPA
                     KRDRLLEIMSQQGRQLARLVDDLMISSELQAGELLITIEMSDLESAVQRGLEGAAPKR
                     RAHQLEVFVDPIRCEIDEYRVAQILRNLIENAYKYTPDRSRVTVSGHATDEGIELEVA
                     DDGPGIPLEKRDLLFEAFRRMEETSAGKEGVGLGLFVVSQLAAAMDALIDLASSTRGT
                     TFTIRIPCRVETIEHRRMGVISGENAIDKEA"
BASE COUNT         1623 a         2741 c         2740 g         1612 t
ORIGIN      
        1 cgcctcactt ctctcgctcc actcgcccgc gctggctcgc gccgcatgga aggagcggat
       61 cgatgggata gtgcggggac gcgcgatcgg cgtcgctgta agggaggccg gcaccaccct
      121 ctaccgccac gcagcaaccc gtcgcagggt gcccgcttcc aaccagaaat tgctgctgac
      181 gatggccctg ctggaccggg tgggggcaga gttctcgttc accacgtcgg ccgctgcggc
      241 ggagtcgccc gaagatgggg tagtcgacgg cgacctgtgg ttgttgggac gcggtgatcc
      301 gacactgtcg tcggagcgtc gctacacgcg gtcactgccg gtggatacga cgaagatcaa
      361 gtcgcttgcg ggggcagtac gtgaggccgg catcactcgc atcaggggca gcgtcatcgg
      421 aagcaccagc tacttcgcgc gcgattggtt cgccatcggc tggaaggccg gatacccgcg
      481 cgatgaatgt cctctgcctt ctgcgctcgc gatcaatggg aacgttaacg atggcggcaa
      541 ccatgtgtcc gatcctgaga tccgcgcggc tcgctcactg tcgcaacgac tcgagaagat
      601 ggggatccgg gtgagtggac ctcccggtgc ggctgaggaa cctcccggac tcgaggagat
      661 cgcaagcgtc ctatcggaac ctctgccatc gatccttcaa catctgaacg tgaactcgtc
      721 gaacttcttc gccgagatgc tcggtaagag gctcgcggtc gaagcgcgtg gacgaccggg
      781 gacgatcgcg aagggggccg gcgcggtcga ggggtgggcc gaccgcaacg gcgtgacggt
      841 gatcgcgcac gatgcctcgg ggctctcgta tcagaaccgt gtgtcaccac tgggcatggt
      901 ccggctactc ggtcgcgtcg aacacgagga atggatcgat cctttcagag ggaccttggc
      961 gcgagccgac gaaggaacgc tggagaaccg gttgaacggc gtccgcgtgc gagcgaagac
     1021 cggcacgctg gacaaggtct cgacgttgtc gggttacgtg tggctcagga gccgcgatgt
     1081 gtggggtgag ttctcgatca tgtcgtccgg gatggagaag acgaccgccg ccgacatcga
     1141 aaacaggatc gtgcggatcc tgacgcgctc cgccggctaa aaggaaagga ggcggctccc
     1201 acagggaacc gcctccgctg attggctctc gtttactcga cggtgtccac gctgtcgacg
     1261 gagtcgacgt cgtcgtcgac actgggttcg ctcatcgaat ccaccgagtc gtcatcggca
     1321 tcctagcctt cgtcaccctc ggcagcgtct tcttcttcct tggcgttctc gtcacggagt
     1381 gtgtcggtgc cggcttcgtc ggactcctct tcttccgaat cacagtcatc cgaggcgcca
     1441 gacgctgcag tgaccatttc tccgtcggtg ggggccgggc gcacgtgcac cttctcgaca
     1501 atcccgtcgt cgagtttcat cctgaggatc caggccccgg gtttgaggtc ggtaacgctt
     1561 ccgcgcgacg ggttgccgtg tcccttgcca cgagcatgac ggccgcggtg ctcgacccga
     1621 accttcacgt cacatgcgac ggggcccgac caagtggtgg tgtcatctag ggcgaccgac
     1681 agggtcgtgc cgtcgaagga ttcgatcgtg cccatggcgc gcttcccgtg ccctctgtgg
     1741 gtcttctcgg tatggtcgcc ggcaacggcc ggggtcgctc cgatgaatcc cgctactgat
     1801 atcgagatca ccgccgccat gatcttttcg cgcatgaagg cctccattcg gtaaccgggc
     1861 ggcctgaaac aggtccctgg ctttgcgtct ccgcctcgcg acgggtttgc tcttgtcgtg
     1921 gaggcgcgct gcatcctcca aggagagctt cggcaggggg taactagttc tttagggtga
     1981 gaaaaaagag gcccccgatt acccgggggc ccttttgatg tctgttcaga gccctataag
     2041 ggctgccagt tagaacagaa gggttagacg acttctcgtt cgcggacccg gtccgtgtga
     2101 acgaccgtgt cgcgccggtt accccagccg ccccaggacg accagaaaat catcgacagg
     2161 acgccaccga gaacaccgac gatcagaagg atgatcccga tcgtgttgat gtcgaatcct
     2221 tcggcgtcga agctgacggc gaaggcgagg atcgctccta gcgctatcag gaaaagactt
     2281 gcaccgatac ccatgggtca ccaacctttg gcttggttgc ttctcttgac ccagacatac
     2341 ccggaaggag ggcggatgaa acagttacgt attcgcgcga tcacccttcg cagtcaggtc
     2401 ggagagaacc gcgcgcgctg cgttgcgccc tggagcccca catacgcccc cgcccggatg
     2461 cgctcccgag ccgcatagat agaggccggc catcggggct cggtagcgat gccacgcagt
     2521 ggccggacgt ttctcgaaca gccagtccgg caacagctcg ccgtggaaga tgtttccgcc
     2581 ggtcagtccg aaacgctctt ccaggtcggg ggggccgaga acgagtcggt cctcgacgag
     2641 gtcggtgagc ccgggggcgt actcctcgag ggtccttaga acgatgtccc cgatctcgtc
     2701 tcggcgcgtg ctccagtcgc cttccgccag gtcgtacgga gcgtactggg cgaagatcga
     2761 cagcgtgtgc ttgccgggcg gagcaagatc gggttcggaa gccgattgca tccacgcctc
     2821 tatgaacatc ttctcggcag gctcgcctac accggcggct cgcgctcctt cttccagata
     2881 atcgatcgaa ggcgagatcg tgatcgtgcc ggtgtgctca gggcccggtt cgttgccccc
     2941 ctccatcccc ttgaagcgcg gcaacccgct gagcgcgcag ttgaccttca ccaccgtccc
     3001 cttggtcggc atcagtccca cgtcttcgag gtattcgggt ggaagatgcg cgtcgcgcag
     3061 aaacccaaca gtgcgcacag gatcggcgtt agagcaaacg atcggggctg agagctcctc
     3121 gccagacacg aggtcgacac cggacgcgcg ccgcctgtca tcgagccgca cgctagcgac
     3181 ctcggcctcg agtcggatct ctccgcccgc cgcgcggaaa cggtcggcga gctctgtcgt
     3241 tatggcgccc atgcccccgc gcaccaatcc ccacacaccc gactcccccg ttgcctcgcc
     3301 gaatgcgtgg tgcagatata tgtacgccgt gccgggagtc ttcggacccg cggccgagcc
     3361 gatgatcccc tgtgacgcca ccaaaccctg catcaggtcc gactcgaagt actcctcgca
     3421 cagatcggag atggagccgt ctacggtgcg cgcgaacagt cgctcgacct cggactggcg
     3481 gaaggcacgt ctggcctgct tacgagtggc cggatacgtg aggagtggcc gcagacgtag
     3541 ggcggcctct tcgaacagct cttcgaaggg caggtaagcg tcggcatcct tcttggacac
     3601 cttcgagatc tcgtcatggg ttctcttctc gtcgcgccat aagaagaggc ctcctccgtc
     3661 ggggaagggt gcgaagacct tcggttcctt gatccgcacg tccagttcca ggccgagctc
     3721 ggacacgatc tgaggtagca gcagagacaa ggagtagctc gccgtcgaga tacggaagcc
     3781 ggggaacagc tcctcggtca cgcaggcgcc cccgatgcgg tcggtgcgtt ccagaactac
     3841 aaccttcagc ccggcgcgct gaaggtaact cgcacacacg agtccgttat gtccggcacc
     3901 tatgacgatc gcatcgaagt tagtcagagc acggcctccg ggcccggtcg ccgtcgtgac
     3961 cgaagcaggc taccctttca gcgatggcga tctccgcgtt ctgcaccaca tgccaacgca
     4021 ccgtctacgt tgaagaagga gacacacccg tgtgcccggt gtgctcttcg cctctgctcg
     4081 agacggtcga gagctcgtca gaggacgacg cggtcgcgac cgatcagtcg cccggcgcga
     4141 agtaagacac agataccgcg tctccacctt cgataacgac gctcgacacc gaccccgtcg
     4201 agcaaggggt gaaccccatc cacggtggta cccggctctt cgttagtgac aagcgagcca
     4261 cgatgacggg cgtctggtgc gagacgacga ccagctcccg gccccccgac gaacgaaccg
     4321 cctcgtcgat cgcgtccagc atccggacac ggatctcggc aaacgattct ccccacgacg
     4381 gccgccacgg attgcgaaag ctccaccaca cacgcggaga ccgtgcgaac gtggcgatgc
     4441 tgcggccgac accctcgagg gtcgaatcgc tctcgatcag tcggtcgtcc gtcacgatct
     4501 cgacgtcgtg gtgcgccgcg atcgcgcgtg ccgtttcctg agcgcgctcc aagggcgacg
     4561 accagatcgc tccaacgtcc ctgtctccca agtgttgggc cgctccttcg gcctgtcgaa
     4621 ggccgcggtc ggacaggtga aatccgggca aacgtccata gatgatcccc ttcgggttct
     4681 caacccgacc gtgacgcacg agatggatgg tggcgttcat cgggggtgag gctattcttc
     4741 ttagacatgc cagaagtgct gctctttgga gcaaccgggt ataccggacg actgacggca
     4801 caggcactgc accggcgcgg agccaacttc gccatatcgg gcagagacca tggcaagctc
     4861 gaagctctcg cgacgacgac cggcaacccc gagatccgcg tggcatcggt aggagatgtg
     4921 cgtggactcg cagccgcgct ggaggacgtc aaggtcctca tcacctgcgt gggccccttc
     4981 gaacaactcg ggtcgaccgc ggttgaagcc gctcttcagg caggggtgca ctacatcgat
     5041 tccaccggcg aaggtccctt catcgctcgc ctcatcgaga acaagatgga gcaagcgcgc
     5101 aaagcaggga tagcgatggc acccgccatg ggattcgacg aggtacctgc cgatgtcgct
     5161 gcgactcttg cctcgcgcga cttagatcct gtcgacctca ctctgaccta tgccgtgcca
     5221 ttgacgccat cggccggaac tcttcgaagc gcgctgggga tcctttcgga acccgcggcc
     5281 tggatcgaga acggtcggcg ggtcaccgtg aaggcgggac aacggagccg ctgggcgccg
     5341 atgcctcctc ctctcggccc ccgatcgagc gtcgccttcc cactcgcgga aggtcatctc
     5401 gcgccgcttc atctagaact cgactcgttg cgcgtttacg ccacggtcgg tatggcccag
     5461 cgtctcgggt tgaagttggg ggggccgttc ttgccgctgc ttaagtccgg gccgatcaag
     5521 agtgtcaccg aaagcttgat cgggcgtcta cctgaaggac cgacggacca acaacgccgc
     5581 gcgggccagt ggacgatcct tgcggaagca cgctccggcg acgcgtggcg aaacgtcgca
     5641 ctgaccggaa ccgatgtgta cggactcacc gccgagttcc tatctgccgc ggccctgaga
     5701 ttcgcgggag acggacctct cgaaaccggg gttgtcgctc cggtgcaagc cgtcggctta
     5761 gaggtctggg agaaggaatt cgcagatcac ggagtaacga tccaaacgtt cgaaccgcac
     5821 taggagtcaa aggagaccca tgggagcgag actcgcctac gcaaaggtga tcgaccgcca
     5881 ggtgttctac gagaagggtg gcaacctcca tcccaagctc gagaacgagg tgctcgtgca
     5941 agcagaaccc ggcaaggcgg gagctttcct cgttttccga gcctgggccg acgatcacgg
     6001 ggccttcact gagcaatggg cgatcaagga acccgaggga cgaacgatct accagagctc
     6061 gcccagagag atccatatgc ccacaccgag ccacacggaa cgcctggagg acgaggttgc
     6121 ggacctcgtg ctggactttg ccgccgacga ctaccagctc gtcttcttcc tggacgagcg
     6181 ggagatcgcg aggatgaact tccccgtgaa ggtgtcctcg aacgggaacg gatgaaagag
     6241 aaaggcgggc gggagtcgtc cgacgcgcgg gcagtgcgtc ggatggctgc ggcggcgaat
     6301 gcgactggac ccccgccctg gcttcaaatt ctgagggagc cgcagggccc tgcgatatag
     6361 gcatttctgc ctatcttgct tcgcctaaga aagggagaaa tcggaacagt gcgacgggaa
     6421 cggacgatga aaacctggtt gatcgcaacg ggcgccatct atgccttggg ggcggtcgat
     6481 ttcatcgcgc gtccgtgggc tgcgacggcg tctctcggca acgtcggagg cgacgagatg
     6541 gagaccgaga gcgtgggtct gtacaacgcc ctcgcatcgt cttacatggc aacgatcgca
     6601 gccctgtctt tcagcgcagc aagggatcct ttggacaaaa gggacttgat cccacctctc
     6661 ctcgtcgcca aagccgtgtc gtcggcgacg atgatgtacc gctacttcac cactaagaaa
     6721 cgcggctacg cctatggcgc cgccctggat gccttcctcc tcgggaccac ggcgggcctg
     6781 gctgcctcct tagaggaggg ctgatcgtct cgacgatcag ccgaaaccca atgacacctc
     6841 tgtaacccgc cccctcgggc cggcgtctaa cgaaagcttc gggcggaatg gccgcccgag
     6901 acgtcacgga ggtgacacgt gtacgagttc gcaatcgtgg ttctgctcgg catcgggact
     6961 ttcaaagtca tcgacatgct gaacgagtac gtggacctcg ccaaagttca gtcgatcctg
     7021 accatcgctc ttggcgcggt cgtttgttgg gccctcggat tctcgttgtt cgagcactgg
     7081 ggcatggcag tacgcagcga ggcgatgggc tatctcggca ccggcctcat ggtttcggca
     7141 gccggatatg cgaccccgaa ggtcttcgag cacctagccg aagtcatcgg gcatcccaga
     7201 agccacacgc actccagagc tgcctagcca cgtaacttcc cctcggaaag ggcccgggtt
     7261 ccccgggtcc ttttcttttc catctcctct tttccgccct tctgtgccac ccttgtattt
     7321 ggtgaacgac ccgatctatc tgcggcgact cgaaagccgc gtgacgctgg tccgagcggc
     7381 gttctgcgcc ggcttgctcg ccgcgtgggc taccgggtcg ttgccgaccc aagagacttc
     7441 gggtgcggtg ctgagagccc tcggtcccgt tctcgcgttg ggaacattgg cgatcgcatt
     7501 cgcggcgcgg ggcttgcggg gagatcgggc ccggacgcga ctcggagtta ccgcattctt
     7561 gttcgacgtt tcgatcgccg ccgtggctgc cctcgcggcc ggcgagcaga tcgccatcgc
     7621 gtttgtcttg gtcatgatcc ctatagaagc ggcgctccgc tacgggctaa ggccgtcgat
     7681 cgttgtggcg atcggagtgt ccctcctctt tggagcggtg tggtgggatt cccccgccac
     7741 gatcgcgttg gttggcggtg tctgtctcgg gacggcgtgg gtggtcggta gcgccgtaca
     7801 gatatgggtc gagaaacaac gtttcctcga gcgtcagacc gcgcaactca tcgaacacga
     7861 ccggttgcgc gatcgaaacg ttgccatcac ttcgcacgag atccgcagcc cgctaacggc
     7921 ggtcatcgca gcaaccgacg tgctgaaaag gaaaggcgac gaaatcgaac ccgccaagcg
     7981 ggatcgcttg ctcgagatca tgtcgcaaca aggccgtcag ctggcgcgtc tggtggatga
     8041 cctcatgatc tcttcggagc tccaagccgg cgagctcttg atcacgatcg agatgagcga
     8101 cctggagtcg gccgttcagc gcggcctgga gggtgcggcc cccaagcgac gcgcgcatca
     8161 actcgaggtg ttcgtggatc cgattcggtg cgagatcgac gagtatcgcg ttgcccagat
     8221 cctcaggaat ctcatcgaga acgcgtacaa gtacacaccc gatcgctccc gcgtcaccgt
     8281 gtccggccat gcgaccgacg agggcatcga gctcgaggtc gccgacgacg gccccggtat
     8341 ccccctggag aagcgtgacc tccttttcga agctttccga aggatggagg aaacctcggc
     8401 cggcaaggag ggcgtcgggc ttggtctgtt cgtggtgagt caattggccg ctgcgatgga
     8461 tgcactcatc gaccttgcct cctccacgcg tggaacgacg tttaccatcc ggattccctg
     8521 ccgtgtcgag acgatcgagc accggcggat gggagtcatc agcggcgaga acgccataga
     8581 caaggaggct tgagtgccga ccaccgacct caatggagtg aagctcaact acaacgacac
     8641 cggaggcgac ggggcccccg cgttgttgct gcacgcgttc ccgttcagtt cgaacatgtg
     8701 ggagccacag atcgag
//
LOCUS       DATMED010000258         2993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4041152, whole genome shotgun sequence.
ACCESSION   DATMED010000258 DATMED010000000
VERSION     DATMED010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4041152"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2137
                     /locus_tag="VNP73_06625"
     CDS             <1..2137
                     /locus_tag="VNP73_06625"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HWL65633.1"
                     /translation="VFLPLVAQAGEKSSDWCARHENHKKCEDPSTGSGVTIEVLSNRA
                     DLVSGGDALVEITSASGVPADLAVTLNGSDVTSSFATRSNGRYLGVVSGLAEGANDLV
                     ASSPTGAGARLPITNHPIGGPIFSGPQLQPWICTTAAAGLGDPIDDQCNAPTKVEYLY
                     QAEGSTAFTPYDLENPPSDVRSITTDQGNTVPYIIRQETGTQNRGIFRLAVLFDPSEQ
                     WTPWEPQAGWNHKLYYPFGASCGTVYSQSSAQNVQNNNALSRGFMVATSSLNVLANNC
                     NTVLSAESVVMLKEYIVDNYGEIRYTFGTGSSGGSIGQNMVANSYPSLLQGITEGANF
                     ADTVTTGMEVFDCHLLHQYFTGKSQWNAAAMASVTGSGTSAGTCAGWETFFAAVENPR
                     DGAGVPPEQAYDPETNPGGARGKYDDFEQNVWGLRPPEEWGPQEQAIGRGFAKPLYDN
                     IGVQYGLNALRDGLIATEQFVDLNEKIGGFDIDFNLTPERSVGASTASGIAYQAGRIN
                     DGAYLDEVAMIDQRGTGNIDPLLIHTMHHSFALKDRIEESNGGADNHVVWRGGSPAPA
                     FDVMDQWLTAVEADDSDQSLAEKVVANRPAEAEDMCFVGDQAFTDKQTCDILWPYYGA
                     PRIVAGGPTTHDNIKCELTPLDRSDPTYGPVPFTDAQWARLQEVFDSGVCDWSKDGVD
                     RQVSVPWMTYSDGPGTGHPLPESPTSQPL"
     gene            2147..>2993
                     /locus_tag="VNP73_06630"
     CDS             2147..>2993
                     /locus_tag="VNP73_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65634.1"
                     /translation="MKRSFATILLSALIAGSVTVGVPSASGGIVAESKDFRLIESVTI
                     GGVAVAGQVVGDTYFISSWQTGLFSYDVSNPKQPVELDHMSADEIQIQSNENEDLATN
                     GEILLLSQFNRTDATNRLLVVDVRDPAEMNVISTLPGAGGHTLDCLYDCKWAYASSSG
                     ADSRGVIIDLRNPKQPKLVDKLWSDAMGGVSAHDVTEVRPGLVVTSSTPLFVLDVTDP
                     TKPRALMKTQDDAPNTGHNNIWPRKGKDRFLITASEGVNNGRCEMYGDDGKTLQVWDT
                     RGWKRG"
BASE COUNT          601 a          937 c          916 g          539 t
ORIGIN      
        1 cgttttcttg cctctggtcg ctcaggcggg ggagaagtcc tcggactggt gcgcaaggca
       61 cgagaaccac aagaaatgcg aggacccctc aaccgggtcc ggggtcacca tcgaggtcct
      121 ttctaatcgg gccgacctcg tctccggcgg tgacgcgctc gtcgagatca cctctgcttc
      181 gggggtcccc gcagacctgg ccgtgactct caatggttcc gacgtcacgt ctagctttgc
      241 cacccgctcg aacggtcgct acctgggagt cgttagtggg ctcgccgaag gggccaacga
      301 tctcgtggcg tcatctccca ccggtgccgg cgcgcgcctg ccgatcacta accatccgat
      361 aggcgggccg atcttctccg gaccgcaact gcaaccatgg atatgtacga ccgccgcggc
      421 cggcctaggt gatccgatcg acgaccaatg caatgcaccg accaaggttg agtacctcta
      481 tcaagcagag ggttccacgg cctttacgcc ctacgacctc gagaacccgc cctcggacgt
      541 gcgctcgatc accactgacc agggcaacac ggttccgtac atcattcgcc aagagaccgg
      601 cactcagaac cgcggcatct ttcgcctcgc cgtgctgttc gacccgtcag agcagtggac
      661 gccgtgggag ccccaggccg ggtggaacca caagctctat tacccgttcg gcgcaagctg
      721 cggaacggtc tactcgcagt cgagtgcaca gaacgttcag aacaacaacg cactctcgcg
      781 cggcttcatg gttgcgacgt catcactcaa cgtattggcg aacaactgca acaccgtgct
      841 ctcggctgag tccgtggtga tgctgaagga gtacatcgtc gacaactacg gcgagatccg
      901 gtacacgttc ggcaccggat cttccggcgg ctcgatcggc caaaacatgg ttgccaactc
      961 ctatcccagc ctcctgcagg ggataaccga gggagcgaac ttcgccgaca ccgtgacgac
     1021 gggcatggaa gttttcgact gccacctgct gcatcagtac ttcaccggga agtctcagtg
     1081 gaatgccgcc gccatggctt ccgtaacggg aagtggcact tcggcaggca cgtgtgcagg
     1141 ttgggagact ttcttcgccg ccgtggagaa cccgcgtgac ggagcgggtg tccctcctga
     1201 gcaggcatac gaccccgaga ccaaccccgg cggtgctcgc ggaaagtacg acgacttcga
     1261 acagaacgtg tggggcctgc gaccgccgga ggaatggggt cctcaggagc aggcgatcgg
     1321 acgtggtttc gcgaagccgc tgtacgacaa catcggtgtc caatacggac tcaacgcctt
     1381 gcgggacgga ctcatcgcga ccgaacagtt cgtcgacctc aacgagaaga tcggcgggtt
     1441 cgacatcgat ttcaacctga cgcccgagcg atcagtgggc gcgtctaccg cctcaggcat
     1501 cgcctatcag gcgggccgga tcaacgacgg cgcctatctc gacgaagtag cgatgatcga
     1561 tcagcgcggt accggaaaca tcgacccgct attgatccac acgatgcatc actcattcgc
     1621 tctgaaggat cggatcgagg agtcgaatgg gggcgccgac aatcacgttg tctggcgcgg
     1681 agggtcaccg gctccggcct tcgacgtcat ggaccaatgg ttgaccgctg tggaagccga
     1741 cgattcagat cagtctctgg ccgagaaggt agttgccaac cggccggccg aggccgagga
     1801 catgtgcttc gtaggcgacc aagcattcac cgataagcag acgtgcgaca tcctgtggcc
     1861 ctactacggt gcgccgcgga tcgtcgccgg cggcccgacc acgcatgaca acatcaagtg
     1921 cgagctcacg ccgctcgacc gtagcgatcc gacatacggg cccgttccct tcaccgacgc
     1981 gcagtgggct cgtctgcagg aagttttcga ctcgggcgtg tgcgactggt cgaaggacgg
     2041 agtcgaccgg caagtgtcgg tcccgtggat gacctattcc gatggtccgg gtaccgggca
     2101 cccgctgccg gagtccccga cgtctcaacc tctttaggag catctgatga aacgatcctt
     2161 cgccacgatc ctgctctctg cgctaatcgc aggatctgtg accgtcgggg tgccgtccgc
     2221 ctcgggcggc atcgtcgcgg agtcgaagga cttccgtctg atcgaaagtg tcacgatcgg
     2281 cggagtggcc gttgccggcc aagtggtcgg cgacacttat ttcatctcca gctggcagac
     2341 cggtctgttc tcgtacgacg tgtccaaccc caagcagccg gtggagctcg accacatgag
     2401 tgccgacgag atccagatcc agtccaacga gaacgaagat ctggccacga acggcgagat
     2461 cctgctgcta tcccagttca atcgaaccga tgccaccaat cgtttgctgg tcgtcgacgt
     2521 ccgggatccg gccgagatga atgtcatctc gacgctcccc ggcgccgggg gacacacgtt
     2581 ggactgcctg tacgactgca agtgggctta tgcatcgagc agcggcgcgg acagcagggg
     2641 agtcatcatc gatctgcgga acccgaagca gccgaagctc gtggacaagt tgtggtcgga
     2701 cgcgatgggg ggcgtttcgg ctcacgacgt gaccgaggtg cgacccggtt tggtcgtgac
     2761 gtctagcact ccgttgttcg ttctcgatgt gacggatccc acgaagcccc gcgctctcat
     2821 gaagacgcaa gacgacgcac ccaacacggg tcacaacaac atctggcccc ggaagggcaa
     2881 ggatcgcttc ctcatcacgg cgtccgaggg cgtcaacaac ggccgttgtg agatgtacgg
     2941 ggacgacggc aagaccctgc aggtctggga tacacgcggg tggaaacggg ggg
//
LOCUS       DATMED010000259         2219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1348366, whole genome shotgun sequence.
ACCESSION   DATMED010000259 DATMED010000000
VERSION     DATMED010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348366"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VNP73_06635"
     CDS             <1..624
                     /locus_tag="VNP73_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWL65635.1"
                     /translation="SHVHAIRGNADRELVEGYDQASKGGGAGEEDDIWGLRAAWTAQQ
                     ITTDQRDFLAGLPETKTFDVMGIGLVRFCHGSPRSDEEIITAVTSDARLRRILAGVEE
                     ELVVCGHTHIQFDRVVDGTRVVNAGSVGMPYEDEPGAYWALFGPEVSLKRTVYDFDEA
                     AERIRNSGFPEADEHARQLFVELLSREETAQFFERLAKEREEAGQEG"
     gene            669..2153
                     /locus_tag="VNP73_06640"
     CDS             669..2153
                     /locus_tag="VNP73_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWL65636.1"
                     /translation="MTERKRGRGAATAVIDLNPELEAICGADGVVSDHVARKTYESDG
                     LLQYKVQPGAVVLPTTTEQVSKVIGLCHRLDVPWVARGSGTGLSGGAIPVEDGVLIVT
                     SRMRSILEVDLVNQRVVVEPGVINREISAAVAPTHFYPPDPSSQVVCSIGGNIAENSG
                     GLHCFKYGFTTNYVTGLEIVLPDGSVVELGGKELDAPGYDLIGAFVGSEGTLGLATRI
                     TLRIIPVPETVRTLIAYFKGVGPAGQTVSDIVADGALPGAIEMMDNAAIRACEDSTHV
                     GLPVDAEAALIVELDGPKPECEARFDGVVDLCHRNGATDVHVAANETERQLIWKARKA
                     AFAAMGRIAPNYYVQDGVIPRTRLGEVLERIAALGHEYDLVVANVFHAGDGNLHPLVC
                     YDGTKPGEAERAEECAGLILRACLDAGGSITGEHGVGIDKKRFMGDMFSEDDLATFQR
                     LRCSFDPEQRANPGKVMPTPRLCGEVPGPYRRHPLEVAGIAERL"
BASE COUNT          411 a          680 c          752 g          376 t
ORIGIN      
        1 agccacgtcc acgccatccg cggaaacgcg gaccgcgagc tcgtcgaagg ctacgaccag
       61 gcctcgaaag gtgggggagc cggtgaagag gacgacatct ggggcctgcg cgccgcgtgg
      121 acggctcaac agatcacgac cgaccagcgc gatttcctgg ccgggcttcc ggagacgaag
      181 acgttcgacg ttatgggcat cggccttgtc cgtttctgtc acggatcgcc gcgcagcgac
      241 gaagagatca tcaccgccgt aacctccgac gctcggttgc gccggatcct tgccggcgtc
      301 gaggaagaac tggtcgtctg tggacacacc cacattcagt tcgatcgcgt cgttgacggg
      361 acgcgcgttg tcaacgcggg gagtgtgggt atgccctacg aggacgaacc gggcgcgtac
      421 tgggcgttgt tcggcccgga ggtcagcttg aaacgaaccg tctacgactt cgatgaggcg
      481 gccgagcgga ttcgaaacag cggcttcccg gaggccgacg aacacgcgcg tcaactcttc
      541 gtcgaactgc tttctcgcga ggagacggct cagttcttcg agcggctcgc caaggagcgc
      601 gaagaggccg gtcaagaggg ttgagccggg gttgatctcg cacgcctgag aaggcgtggg
      661 atgcttgatt gacggagcgc aagcgtggga ggggggcagc cacggcagtc atcgatctga
      721 atcccgaact agaggcgatc tgcggcgcag acggcgtcgt ctccgatcac gtcgcccgta
      781 agacctacga gtcggacggg ctccttcagt acaaggttca gccgggcgcg gtggtgttgc
      841 cgaccacgac ggaacaggtc agcaaggtga tcgggttgtg ccaccgcctc gacgttccct
      901 gggtggcgcg cgggtcgggt accggcctct cggggggagc gatccccgtt gaggacggcg
      961 tactgatcgt gacctcgcgc atgcgcagca tcctcgaggt cgatctcgtc aatcagagag
     1021 tcgtggtcga acccggagtc atcaaccggg agatctccgc tgcggtggcg cccacgcact
     1081 tctacccgcc ggatccctcg agccaggtcg tgtgctcgat aggcgggaac atcgccgaga
     1141 actcgggcgg gctgcactgt ttcaagtacg gcttcaccac caattacgtc accggcctcg
     1201 agatcgtgct gccggacgga agcgttgtcg agctgggcgg caaggaactt gatgctcccg
     1261 gctacgacct catcggagcc ttcgtgggct cggagggcac gctcggcctg gccacccgga
     1321 taacgctgcg aatcatcccg gttcccgaga ccgtccgcac cctgatcgcg tacttcaagg
     1381 gtgtggggcc ggccggacaa accgtgtcgg acatcgtggc cgacggcgcc ttgcccggag
     1441 ccatcgagat gatggacaac gccgcgattc gcgcctgcga ggactcgact catgtcggcc
     1501 tgccggtcga tgcggaggcc gcgctgatcg tcgaactcga tggacccaag cccgaatgtg
     1561 aggctcggtt cgatggtgtg gtcgacctat gtcaccggaa cggggcgacg gacgttcacg
     1621 tcgccgcaaa cgaaaccgaa cgtcagctca tctggaaggc gcgtaaggct gcattcgcgg
     1681 cgatgggcag gatcgcaccc aactactacg tgcaggacgg cgtaattccg cgcacacggt
     1741 tgggcgaggt actcgagcgc atcgcggccc tgggacacga gtacgacctg gtcgtggcca
     1801 acgtctttca cgccggcgac gggaaccttc atccactggt ctgctacgac gggaccaagc
     1861 cgggtgaggc cgagcgcgcc gaggagtgcg cgggcctgat cctcagggct tgcctcgatg
     1921 cgggcgggtc gatcaccgga gaacacgggg tggggatcga caagaagcgc ttcatgggtg
     1981 acatgttctc cgaggacgat ctggcaacct tccagcgact gcgctgctcc ttcgaccctg
     2041 agcagcgagc gaatcccggc aaggtgatgc cgactccgag gttgtgcggc gaggtgcccg
     2101 gtccctaccg ccgtcacccg ttagaagttg ccggcatcgc ggagcgactt tgatggcaaa
     2161 acagctggag actcgcaccg agtctccttc gacacctcaa gaggccgccg aggtcatgc
//
LOCUS       DATMED010000260         3460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3651469, whole genome shotgun sequence.
ACCESSION   DATMED010000260 DATMED010000000
VERSION     DATMED010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3651469"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VNP73_06645"
     CDS             complement(<1..701)
                     /locus_tag="VNP73_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HWL65637.1"
                     /translation="MSGVFEHISNDGYEQVVFCSDDQSGLRAIIAIHSTALGPALGGT
                     RFYPYDTEEEALNDVLRLARGMTFKAAAAGLDLGGGKAVIIGDPRRIKTEELLRAYGR
                     FVETLGGRYITAEDVGTALEDMDVVKRESRWVTGCSHTYGGSGDPSPVTAYGVMNGIK
                     ATLLEVFGSSDLKDRTIALMGVGKVGYALCGYLVKEGARVTIADIDVDNLARAVGDYG
                     VATVPLAEIHKQEVDV"
     gene            814..1620
                     /locus_tag="VNP73_06650"
     CDS             814..1620
                     /locus_tag="VNP73_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65638.1"
                     /translation="MIKTAYLRVYQPLGSFPNDEQPHWAAPPPESEIRDAYAARRWLL
                     SAALPENPPILGGTQGAFVRRVGDEVLVCPWRTRLRMLAGLLAFRNSIPEEVAEAFVP
                     EEEARRAAHELAELGEAHPEVRSHILHANWHVPLRWFAAFEDSERILTEDKDGLRIRY
                     ETKLSEAIARLRKGLEILENSWVDESVTGAVRELVEWLEDFGTDGLLEIDYASVASMF
                     SDEDLLEDRSAGEVSSCLEALQAEDVVRAGRLFGTLTERWTDARSQEVLN"
     gene            1808..2002
                     /locus_tag="VNP73_06655"
     CDS             1808..2002
                     /locus_tag="VNP73_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HWL65639.1"
                     /translation="MKAQKDDALLTPAEVAALFRVDPKTVTRWAKAGKLSSIRTLGGH
                     RRYRADEVKKFLEGAKQQLG"
     gene            complement(2105..2177)
                     /locus_tag="VNP73_06660"
     tRNA            complement(2105..2177)
                     /locus_tag="VNP73_06660"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2142..2144),aa:Phe,seq:gaa)
     gene            2292..2366
                     /locus_tag="VNP73_06665"
     tRNA            2292..2366
                     /locus_tag="VNP73_06665"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2327..2329,aa:Asp,seq:gtc)
     gene            complement(2387..>3460)
                     /locus_tag="VNP73_06670"
     CDS             complement(2387..>3460)
                     /locus_tag="VNP73_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWL65640.1"
                     /translation="LARSDLLGALPPAALEELAGHVRLNEYRAGQVIFREGDLGDSLH
                     IVRSGLVKVVRPGAEGREVTLSTMGPGKTFGELAVLDPAPRSASVVAIDLTHTIEISK
                     AELEELLDRDPGLSRQLLATMARSLTVAKEEVTKQNQVLDLKVQERTQELQESQLEVI
                     RRLGRAAEFRDDDTGLHISRMSRFCARLAAEAGFTDQDCELLLQAAPMHDIGKIGIPD
                     RVLLKPGKLDDEEWEIMKTHPMLGAELLSGATSKVMQLAQGIARAHHEKWNGKGYPDG
                     LKYEDIPILARICTISDVFDALISERPYKNAWTVDDALELIDKQAGEDFDPHLAKLFV
                     GLEPDLRDILARQHELLGEAGQA"
BASE COUNT          657 a         1094 c         1063 g          646 t
ORIGIN      
        1 acgtctacct cctgcttgtg gatctcggcg agcggcaccg ttgcgacccc gtagtcgccg
       61 accgcccggg cgaggttgtc cacgtcgatg tccgcgatcg tgaccctggc cccctctttg
      121 acgaggtaac cacacaacgc gtagccgacc ttgcccacgc ccatcaacgc gatcgttcgg
      181 tctttgaggt cgctcgagcc gaacacttcg agcaaagtgg ctttgatccc gttcataacg
      241 ccgtaggcgg tgaccggcga gggatcaccg gagccaccgt acgtgtgcga acagccggtc
      301 acccagcgcg actcgcgctt gaccacgtcc atgtcttcga gagccgtccc gacatcttct
      361 gccgtgatgt agcgaccacc cagcgtttcg acgaaccgtc cgtacgcacg cagcaactcc
      421 tcagttttga tcctgcgtgg gtcgccgatg atcactgcct tgccaccgcc gaggtccagt
      481 ccggccgcgg cggccttgaa cgtcatcccg cgcgcgagcc ggaggacgtc gttcagtgcc
      541 tcttcttcgg tgtcgtacgg gtagaaacga gtcccgccga gggccggccc caacgcggtt
      601 gagtggatcg cgatgatcgc tcgtaggccg gattgatcgt ccgagcagaa gacaacctgc
      661 tcgtaaccat cgttactgat gtgttcgaaa acgccactca tctagcccgg tcccctttga
      721 ctcggcgatg acgaaacgga ataacaagat ggtaccgccc agatttgacg gtcgatccgg
      781 tcgttcccac gcctgcgtct gtacgatgtg cgtgtgatca agaccgctta cctgcgcgtt
      841 taccaaccgt tgggctcgtt cccgaacgac gagcaacccc attgggccgc ccccccgccg
      901 gagagcgaga tccgagacgc atacgcggcg cggcgctggc ttctttcggc ggcgctgccc
      961 gagaaccctc cgatcttggg gggaacgcaa ggggcgttcg tgagaagggt cggagacgag
     1021 gtgttggtgt gcccgtggcg cacgcgcttg cggatgctcg ctggtcttct cgcctttaga
     1081 aacagcatcc ccgaggaggt cgccgaggct ttcgtccccg aggaggaggc ccggcgcgcc
     1141 gctcacgaac tggccgagct cggagaggcg catcccgagg ttcgctcgca catcctgcac
     1201 gccaactggc acgtcccgtt gcgctggttc gctgcgttcg aggattccga gcggatcctt
     1261 accgaggaca aggatgggct acggatccgc tacgagacga agttgagcga agcgatcgcc
     1321 cggctccgta agggtcttga gatcctcgag aacagctggg ttgacgaatc ggtcaccggt
     1381 gccgtgcgcg agctggtgga gtggctcgag gacttcggaa ccgacggcct ccttgagatc
     1441 gactatgcga gcgtcgcatc gatgttcagc gacgaagacc tcttggaaga tagatctgcg
     1501 ggagaggtgt cctcatgtct cgaagcgctc caagcggaag atgtggtgcg cgccggcaga
     1561 ctgttcggaa ccctcaccga gcgatggacc gacgcgcgtt cccaagaggt gctgaactag
     1621 ggcacatctc acatatacgg actagtagta cttaactagt aacccgtagt ccccaaaacg
     1681 cccaaaatga ccccatctag ccatttgatg agggcacgaa tcggacgatg atgccaacta
     1741 cggcgcaaat caattgattc ggccgtatcg aggggtcgga cgggccgacc caggaatgga
     1801 ggcaaggatg aaggcgcaga aagacgatgc ccttctcacg cccgccgaag tggcggcgct
     1861 attccgcgtc gaccccaaga cggtcacccg ctgggcgaag gccggaaagc tctcctcgat
     1921 cagaacgctc ggcggacatc gccgttaccg tgctgacgag gtcaagaagt tccttgaggg
     1981 agccaagcag cagctcggct aaggcactgc ggcgtagccg caagtagtgc cgtgtcgatc
     2041 cgctcggcta attaaaggga tcgaaaagag gcgccccccc gaagggtgcc tctttttcgt
     2101 tgagtggcga ggggcagaat cgaactgccg acaccgcgat tttcagtcgc gtgctctgcc
     2161 aactgagcta cctcgccgaa acgttggaac aacggacgat atcaagggtg tttctacgct
     2221 tctgctagtt tctctgagtc tgtgactgag cccgagcgcc ggtgggttcg gccgccggcg
     2281 agaacaaact tgccccagtg gagcagctag gagtgctcat ctccctgtca aggagaaggt
     2341 cgccagttca aatctggtct ggggcgcccc gcagcccgtt tagctactag gcctgacccg
     2401 cttcccccag cagctcgtgc tggcgagcca gtatgtctct caggtccggc tcgagaccca
     2461 cgaacagctt cgcgaggtgc ggatcgaagt cctcgcccgc ctgcttgtcg atcagctcga
     2521 gcgcgtcgtc gaccgtccag gcgttcttgt agggacgctc cgagatcaag gcgtcgaaga
     2581 cgtcggagat cgtgcagatc cgggccagga tcgggatgtc ctcgtacttc agcccgtcgg
     2641 ggtatccctt gccgttccac ttctcgtgat gcgcacgggc gatgccctga gcgagctgca
     2701 tcaccttcga agtcgcgccg gagaggagct cggcccccag catgggatgc gtcttcatga
     2761 tctcccactc ttcatcgtcg agcttgccgg gcttcaagag aacgcggtcc gggatcccga
     2821 tcttgccgat gtcgtgcatc ggagccgctt gaagcaacag ctcgcagtct tgatccgtga
     2881 agccggcctc cgccgccaga cgagcacaga agcgactcat gcgcgagatg tgcaagcccg
     2941 tatcgtcgtc gcggaattcg gccgcacggc cgagccggcg tatcacctcg agctgcgatt
     3001 cctgcagttc ctgcgttcgc tcctgcacct tcaggtcgag gacctggttc tgcttcgtga
     3061 cctcttcttt ggcgaccgtg agggaacgag ccatcgtcgc aagcaactgg cgagacaacc
     3121 ccgggtcgcg atcgagaagc tcctccagct cggccttcga gatctcgatc gtgtgggtca
     3181 agtcgatggc aacgacgctc gcagagcgag gagccgggtc gaggacggca agctcaccga
     3241 acgtcttgcc cggccccatc gtcgagagag ttacttcgcg gccctccgcc cccgggcgca
     3301 caaccttcac gagacccgaa cgcacgatgt gaagtgaatc cccgagatcc ccctcgcgga
     3361 agatcacttg cccggcccgg tattcgttca ggcgaacatg gccggcaagc tcctcgagag
     3421 ccgccggagg gagcgctccc agcaggtccg agcgcgcgag
//
LOCUS       DATMED010000261         9089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1355694, whole genome shotgun sequence.
ACCESSION   DATMED010000261 DATMED010000000
VERSION     DATMED010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355694"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VNP73_06675"
     CDS             complement(<1..484)
                     /locus_tag="VNP73_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65641.1"
                     /translation="MLNKLLWTGKFLVALFLFVGALQVMKTGAGELGALQHGGLLVKN
                     AVSTLGLGWVGALIVLSGSPVAASALSLVASGAITEIEGFTMVTGSRLGAAFVVLLVA
                     VIYALRGGEGKRMAPVSTAVMALSTTALIYVPGALLGLGLLGWGPFQRVSITFPSQFS
                     D"
     gene            616..2298
                     /locus_tag="VNP73_06680"
     CDS             616..2298
                     /locus_tag="VNP73_06680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWL65642.1"
                     /translation="MRVLVAAVLVAAQLSAAYGTPTRTISVPITLARTLSLSTPGTVG
                     LDLHATHIAFTWRGESASRIRFRVTSSDGSTSEWQQVPEVHGPAGARHRHFSAVIAVP
                     HAVAVEWRKEKGVGRVILDYLNTLDGPRREVEIPQVAEADAGDPDIVTRRQWGADESL
                     KSKSGGCERRFFNLQQIFVHHTAGANYDQHPRATMRAIYWYHVARQGWCDIGYNFVIG
                     PDGTIFEGRWARGYRPWETHDSESTGGNVVAGAHVANYNSGSLGISLMGNYSQVDIAP
                     AARESLVRLLAWETDRHDLDPQGRHTYRNPDSGSTRKLHYISGHRDAGDTECPGRNVY
                     SELPSIRSETVLAQGSDRDSAFMRLAPVDRRARYGEAATFTGTLLDEVDTPLPARSIS
                     SYYRIAGDRWRRGPETVSDGEGNFTFEIPSERNVQAIATFEGDAYYWGAQSPIARVLV
                     GPEVTIEAEDGTVDAAGVSHHSGDEVVLSGSVIPFHTKNSVTLRMSRLDDDGTYVFLE
                     ERVVELDDDSSYETAWMVPDEDGGSYRAVAIVTGDADHAPSRSAPIFLVVDR"
     gene            complement(2567..3049)
                     /locus_tag="VNP73_06685"
     CDS             complement(2567..3049)
                     /locus_tag="VNP73_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWL65643.1"
                     /translation="MKAARRSFTGALCSILFLTLLPATSANAACYKPTRAERSFVRKM
                     NKARDAAGKAPLSLDREVSKVSKVHTKEMSNEDSLFHTSTSVLSKRVTNWILLGENVG
                     VGGSVSSLHQAFMNSPAHKANILLGSFKYVGVGVIKTDDRMWVTVTFEAKSDPGSPLC
                     "
     regulatory      complement(3066..3157)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3328..3798
                     /locus_tag="VNP73_06690"
     CDS             3328..3798
                     /locus_tag="VNP73_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016891984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWL65644.1"
                     /translation="MGRRFFRHGELPLVLLALLADRQMHGYELMAELGRLFAPHYSPS
                     PGSVYPAVEALESEGLIRPQGEGGPRVYELTPSGKQALEKRRELLAAIELRTGLRVQQ
                     RGTIDAVLDRFASRIRELAGRLEPDSLERILDSTADEIEGKAVSGQAASVEVKA"
     gene            3795..4094
                     /locus_tag="VNP73_06695"
     CDS             3795..4094
                     /locus_tag="VNP73_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HWL65645.1"
                     /translation="MSDAFDRAVEREQSERRRWRYRGAWKRFGVHLRVYLFVNLLLAA
                     IWAAEAVVDGGHPFWPVHALWGWGIGLVVHYLVVTRVTGRWRPRRGHPSTTTQIS"
     gene            complement(4117..6525)
                     /gene="glnD"
                     /locus_tag="VNP73_06700"
     CDS             complement(4117..6525)
                     /gene="glnD"
                     /locus_tag="VNP73_06700"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWL65646.1"
                     /translation="MKAARPVALSDGAQAARQRAVDVDRALFALVEENLGDQPDLSVV
                     AVGGYGRGELSPHSDIDLLFLLPPKSEVTKATLRGLLYPLWDAGWQVGHAARTPKEVI
                     DRTEADLDSATAVLSARPIAGDEALFQEFLDRRERWLRKSGRTIARRIIDATAERHRR
                     VDRAGWVLAPDLREDIGGLRDLHRIAWLRKIAGDETPLPVELAGGGELLLAVREALHG
                     QQKRKGDALRIDLQPKVAAAMDVDADSLMFEVHSVARTIEHRGELISSRLAARVLGGP
                     KRSGWVKRLGHRIRLEEGALALDAGLDPGPVDVLRLLSAHASEGRPIEPATLERVANA
                     LPHDVAPWDQEMRDAFFAILAGEHCISALELLDHIRAWPVLVPEWDRIRALAQHDPYH
                     RYTVDGHLFVVVRELRRAIEIDVLARAAVEEAGDLDTLRLATLLHDVGKGSGHDHSVV
                     GEELARTAAQRMGLSTEEVEEVAALVRWHLLLVDTATRRDLDDGSVIAEVADRMTGRR
                     LRLLYILTVADGLGTGPEGWNDWKATLVAELYRKTLIALETGEIPTRSDVTARAREVE
                     AYEPSLAGRAQDVLATLPHSYLEATSVPDMVDDIRLLLQPPDKGEVRHRVYEGTERDQ
                     AAVTVCVADRPGTLARTAGVLALNRISVLRAQAFSTSAGLALERFVVRVPEAASWESV
                     AADLEAAYSGRLALEARLERKIQDYRPPEPVEPDIRILQDASAHSTVIEVRAPDTLGL
                     LFALTAALSDLDLDIHVAKIDTLGHRVVDVFYVRSAWGEKLTEAQADEVRRSIAHRIG
                     RLFG"
     gene            complement(6527..6865)
                     /locus_tag="VNP73_06705"
     CDS             complement(6527..6865)
                     /locus_tag="VNP73_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWL65647.1"
                     /translation="MKLVVAVIKPFKLDDVKEALEGVGIQGMTVSDTRGFGRQKGHTE
                     VYRGAEYQVDFVPKMRLELVVDDDQAAEVVKAIVTSAKTDAIGDGKVWVVPVEEVVRI
                     RTGEKGPDAL"
     gene            complement(6866..8188)
                     /locus_tag="VNP73_06710"
     CDS             complement(6866..8188)
                     /locus_tag="VNP73_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWL65648.1"
                     /translation="MDTGDTAWILASTALVLFMTPGLALFYGGMVRAKNVLGTLMHSI
                     FAMGLISILWVLIGYTLAFGPDVGGLIGGLDFIGFSGVGGGVHPDGLGATIPHNLFAT
                     YQMMFAVITPALITGAFAERMKFSGYVAFMALWLVFVYSPVAHWVWHPEGWLFKLGAL
                     DFAGGTVVHINAGVAALAAILVIGKRKGFGKEAFVPHNLTMTILGTGILWFGWFGFNA
                     GSALGAGELAATAFLATNLGAAAGAVGWALMEARKERKATTLGVASGAVAGLVAITPA
                     AGFVGPLGGIAIGLIAGCVCSWAVGLKYKLGYDDSLDVVGVHMVGGIVGALLLGIFST
                     AAVNEFGADGLIAGGGLALLGKQLIAVGVTLVFAFVVTYIIAKLVDATIGLRVTEEDE
                     ISGLDLAQHAEVGYSLTETGGGLVTHATGEPSTSQVPNGVPVPRGGEA"
     gene            8450..>9089
                     /locus_tag="VNP73_06715"
     CDS             8450..>9089
                     /locus_tag="VNP73_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65649.1"
                     /translation="MAERPVRAAFPELRHAEAAALNATLIDRALPADQARVASVVRSP
                     LDSGWYRTEVLEVQSDSGEVAKVFLKDFGSVDKGKSRMEDRRERERYFYGRVFPDGSL
                     GLPRYIGEIWGPDRFWLLLEYIDAVPLKWCELDVWFQAAAWLGRFQTMTARKLEELDA
                     SGRFTKHGPEYFENASIRAVKGSARFGADVVSTVERAVPVYLEYAAGLMRPPR"
BASE COUNT         1660 a         2840 c         2953 g         1636 t
ORIGIN      
        1 ggtcactgaa ctgggatggg aaagtgatcg acactctctg gaacggtccc caacccagaa
       61 gaccgagacc cagtaaggcg ccgggtacgt agattaaggc tgtggtgctg agagccatga
      121 ccgcggtcga taccggagcc atgcgtttac cttcgcctcc tcggagcgca tagatcacgg
      181 caacgagcag aacgacgaag gctgcgccga gacgcgatcc cgtgaccatc gtgaatcctt
      241 cgatctccgt gatggcaccc gacgcgacga gggacagtgc cgaggcggcc accggggagc
      301 ccgagagcac aataagggca ccgacccatc ccaacccgag ggtcgacacg gcgttcttga
      361 cgagcaggcc accgtgctgg agcgcgccga gctcaccggc tcccgtcttc atgacctgga
      421 gagcaccgac gaacaggaat agggcgacga ggaacttccc cgtccacagg agtttgttaa
      481 gcatgtcggg ctctcttata acactcctat aggagtgttg ctacaaccaa gccaagccaa
      541 cgcacgcggg agaagtctct tcatctagac gaggcgcgca actactctgg ggggcgcatg
      601 tgggctaggt ggacgttgag agtcttagtt gcggcggtgt tggttgccgc ccagttgagc
      661 gcggcctacg gcacccctac tagaacgatc tcggtcccca tcaccttggc acggacgctg
      721 tcgctgtcca cacccggcac cgtgggcctc gacctccacg cgacccacat cgcgttcacc
      781 tggcgtggcg agtccgccag ccggatccgt ttcagggtca cgtcttcgga cggctcgaca
      841 tccgagtggc agcaggtgcc ggaggttcac ggtcccgccg gggcccgcca ccggcacttc
      901 tcggcggtga tcgccgtccc tcatgccgtg gccgtcgaat ggcgcaagga gaagggggtc
      961 gggcgcgtca tcttggacta cctgaacacc ctggacggcc cccgccgcga ggtcgagatc
     1021 cctcaggtcg ccgaggccga tgccggcgac ccggacatcg tcactcgcag gcagtggggg
     1081 gccgacgaat ctttaaagag caagagcgga ggttgcgaac gacggttctt caacctgcaa
     1141 cagatcttcg tgcatcacac cgccggagca aactacgacc agcacccgcg cgcgacgatg
     1201 cgagcgatct actggtacca cgtggcgcgt cagggctggt gcgacatcgg ctacaacttc
     1261 gtgatcggcc cggacgggac gatcttcgag ggtcgctggg cgcgcggcta ccgtccgtgg
     1321 gagacgcacg actccgaaag cacgggcggg aacgtggttg cgggagctca cgtcgccaac
     1381 tacaactccg gcagcctcgg gatcagtctt atgggcaact actcgcaggt cgatatcgcg
     1441 cccgccgcac gcgagtctct ggtgcggtta ctggcgtggg agaccgaccg tcacgacctg
     1501 gacccgcaag gccgtcacac gtatcggaac ccggactcgg gttccactcg caagcttcac
     1561 tacatctcgg ggcacagaga cgcgggggac acggaatgtc ccgggcgaaa cgtctactcg
     1621 gaacttccgt cgatccgaag cgagacggtg cttgcccaag gttccgatcg cgattcggcg
     1681 ttcatgcgtc tagcgccggt cgatcgcagg gcgaggtacg gcgaggccgc taccttcacc
     1741 gggacactgc tcgacgaagt cgatacaccg ctgccggcac gcagcatctc ttcctattac
     1801 cggatagcgg gcgatcgctg gcgccgaggt ccggaaacgg tttccgacgg cgaaggcaac
     1861 ttcaccttcg agatcccgag cgaacggaac gtgcaggcga tcgcgacgtt cgagggagat
     1921 gcctactact ggggagctca aagcccgatc gcacgagttc tggtgggacc ggaggtcacg
     1981 atcgaggccg aggacggaac cgtggatgcc gcgggggtgt cacatcactc gggcgacgag
     2041 gtcgtattga gtggttccgt gatcccgttc cacacgaaga acagcgtcac ccttcggatg
     2101 tccaggctcg acgacgacgg aacgtacgtg tttctggagg agagggtcgt ggaactagac
     2161 gacgacagct cgtacgagac ggcctggatg gtgcccgacg aggacggtgg aagctaccga
     2221 gcggtggcga tcgtcacagg agatgccgat catgcaccgt cacgctcggc gccgatcttc
     2281 ctcgtagtcg accggtagga gccaggtttc ctggagctca cgcagatgcc gttcggcctc
     2341 gctcttatct agctctacga catccggcga gggaggcgcg gaatcggcgt cgagttcgat
     2401 ggagttctcg gatcgttcgg actgacgctg gggccggccc aatgtctcct ccttatgcga
     2461 ggaatcttgc agtcagtccg ttatgcccta tgaaaatgag agatcggtga aagataggaa
     2521 catttgtacc tattgtgcgg ataaagcttg aaccggttgg ttctagctag cagaggggac
     2581 tgccggggtc actcttcgcc tcgaaggtga ccgtgaccca catccggtcg tcggtcttga
     2641 taacgccgac gccgacatac ttgaacgagc ccagaaggat gttcgccttg tgggcggggg
     2701 agttcatgaa ggcttggtgc aaggaagaca cgctgccccc gacgccgacg ttctcgccca
     2761 gcaggatcca gttggtgact cgcttgctca gaacgctggt cgaggtgtgg aagaggctgt
     2821 cctcgttcga catctctttg gtgtggacct tggagacctt cgagacttcc cggtcgaggc
     2881 tgagtggtgc cttgccggcg gcgtcacggg ccttgttcat cttgcggacg aaggatcgtt
     2941 ctgccctcgt cggcttgtag caggcggcgt tcgcggacgt cgcgggaaga agagtgagga
     3001 aaaggatcga acaaagagcc ccggtgaaac tgcgtcttgc ggccttcacg gcacgaacct
     3061 cccaattcgg cggctgggct gtctgtatgg gacttgctcc cgctacagac cccttgcttt
     3121 gcgcccctgc gtcgccgcag gtttgcctgt tcgttgggtc ttgctcgtac taaatcggca
     3181 tctagagggg ggtccttgag tccccgagca ggggttttct agtcagtcgg gactagttcg
     3241 gggaccggcg agctggggcc gggcgggttt gactccgtaa gagtttcgta ataatctacg
     3301 taagagtttc gtaaggggag gctgtggatg ggtcggcggt tcttcaggca tggggagctt
     3361 ccattggtgc tcttagctct actggcagac cggcagatgc acggctatga gctcatggcc
     3421 gaactaggac gtctgttcgc cccccactac agcccctctc caggcagcgt ctatcccgcg
     3481 gtcgaggcac tggagtccga gggactcatt cggccgcagg gcgaaggggg gccgcgcgtc
     3541 tacgagttga cgccgtcggg gaaacaggca ctcgagaagc ggcgggagct ccttgcggcg
     3601 atcgagcttc gaacggggct gagggtgcag caacgaggga cgatcgacgc ggttctcgat
     3661 cggttcgcct ctcgcatccg cgagcttgcg gggcgtctcg agcccgactc gctcgagcgg
     3721 atcctcgata gcacggcgga cgagatcgaa gggaaggcag tgagcgggca ggcagcaagc
     3781 gtggaggtga aggcatgagt gatgcattcg atcgtgcggt cgaacgtgag cagtcggaac
     3841 gtcgcaggtg gcggtaccgg ggagcgtgga agaggttcgg ggtccacttg cgggtctatc
     3901 tgttcgtcaa ccttttgttg gccgcgatct gggccgccga ggccgtggtg gacgggggac
     3961 atcccttctg gccggtccac gcgctgtggg gatgggggat aggactcgtc gtccactacc
     4021 tagtggtcac acgggtcacc ggccggtggc gaccccgtcg cggccatcct tcaacgacaa
     4081 cccagatctc ttagaagtct ccttgggccg acgggctcag ccgaagaggc ggccgatgcg
     4141 gtgggcgatc gaccgccgga cctcatccgc ctgcgcctcg gtcagcttct cgccccaggc
     4201 gctccggacg tagaagacgt ccacgacccg gtgccccagc gtgtcgatct tggctacatg
     4261 gatgtccagg tcgaggtcgc tgagcgccgc cgtgagggcg aataggaggc caagggtgtc
     4321 gggggcacgg acctcgatca cggtcgaatg agctgaggcg tcctgaagga tcctgatgtc
     4381 gggctccacg ggttccgggg gccggtagtc ctggatcttt cgctcgagtc gcgcctccaa
     4441 ggccaagcgt cccgagtacg cggcttcgag atcggcggcc acgctctccc aggacgcggc
     4501 ttccgggacg cgcacgacga accgctcgag cgcgagtccc gccgaggtcg agaaggcctg
     4561 ggcacgcaac accgagatcc gattgagagc cagcaccccg gcggtccggg cgagggtgcc
     4621 ggggcgatcc gccacgcaca cggtcacggc agcctggtct cgctccgttc cttcgtagac
     4681 acggtgacga acttctccct tgtcgggagg ctgcaggagc aagcggatgt cgtcgaccat
     4741 gtcgggcacc gaagtggctt cgagatacga gtgagggagc gtggcgagaa cgtcttgagc
     4801 gcggccggcg agactgggct cgtacgcctc gacctctcgc gcccgtgcgg tgacgtcgct
     4861 gcgggtcggg atctcacccg tctccagagc gatgagcgtc ttcctgtaga gctcggccac
     4921 gagggtcgct ttccagtcgt tccaaccctc tggtccggtc ccgagcccgt cggcaaccgt
     4981 gaggatgtac agcagccgga ggcgtcggcc ggtcatccga tcggcaacct cggcgatcac
     5041 ggagccgtcg tcgagatccc ggcgcgtggc ggtgtcgacc agcaggaggt gccagcgaac
     5101 gagagcggcg acctcttcga cttcctcggt ggacaagccc atgcgttgcg ccgcggttcg
     5161 agcgagttcc tctcccacca cggaatggtc gtggcccgat cccttcccga catcgtgcag
     5221 caaggtcgcg agtctgagtg tgtcgagatc ccctgcctct tcgaccgcag ctcgggcgag
     5281 gacatcgatc tcgatcgccc gacgcagttc tctgacgacg acgaagaggt gaccgtcgac
     5341 cgtgtagcgg tggtacggat cgtgttgcgc cagtgctctg attcggtccc attcgggtac
     5401 gagcaccggc caagcccgta tgtgatcgag aagttcgagc gccgagatgc agtgctctcc
     5461 ggccaggatg gcgaagaacg catcacgcat ctcttgatcc cagggggcca cgtcgtgcgg
     5521 cagcgcgtta gcgacccgtt ccagggtcgc cggctcgatc ggacgaccct ccgatgcgtg
     5581 cgcagacaaa agtcgcagca cgtcgacggg accaggatcg agcccggcgt ctagagccag
     5641 cgcgccttcc tcgagccgga tgcggtggcc cagtctcttg acccacccgg atcgtttcgg
     5701 cccccccaac acccgggccg ccaaacgcga tgagatcagc tccccgcgat gctcgatggt
     5761 ccgggctacg gagtgcactt cgaacatgag tgagtcggca tcgacatcca tcgcggccgc
     5821 aaccttgggt tgcagatcga tgcgcaatgc atcgcccttt cgcttctgct gtccgtgaag
     5881 ggcctcacgc accgcgagga gcagttcacc gccaccggca agctcaacgg ggagtggggt
     5941 ctcgtcgccc gcgatcttcc tgagccatgc gatgcgatga aggtcccgaa gtccaccgat
     6001 gtcctcgcgc agatccggtg ccagcaccca gccggcgcga tcgaccctcc gatggcgctc
     6061 ggcggtagcg tcgatgatcc gtcttgcgat cgtccggccg ctctttcgca accagcgctc
     6121 tcgacggtcc aggaactcct ggaacagtgc ctcgtcgccg gcgatcggcc gagctgaaag
     6181 caccgcagtg gccgagtcaa ggtcggcttc ggtgcggtcg atcacttcct tcggtgttcg
     6241 agccgcgtga cccacctgcc agcctgcatc ccacaacgga tagagaaggc cccgaagcgt
     6301 tgctttggtc acttcgctct tggggggcaa caggaacaac agatcgatgt cggaatgcgg
     6361 cgacagctcg ccgcggccgt aaccacccac cgccaccacc gacagatccg gttggtcgcc
     6421 gaggttctct tccacgagag cgaacaacgc gcgatccacg tcgaccgcgc gttgtcgtgc
     6481 cgcttgcgcc ccgtcactta gagcgacggg gcgcgcagcc ttcaagctag agagcgtcag
     6541 ggcccttctc tcccgttctg atccggacga cttcctcgac tgggacgacc cagaccttgc
     6601 cgtccccgat cgcatcggtt ttggccgagg taacgatcgc tttcacgacc tcggcggcct
     6661 ggtcgtcgtc cacgacgagc tcgagcctca tcttcggcac gaagtcgact tggtactcgg
     6721 cccccctgta gacctcggtg tggcctttct gacgtccgaa acctctcgtg tcgctcaccg
     6781 tcatcccctg gatgccgacg ccttcgagtg cttccttaac gtcgtccagc ttgaacggtt
     6841 tgatcacggc tacaaccagc ttcatctacg cctcaccgcc cctcgggacc ggaaccccgt
     6901 tcgggacctg cgaagtagag ggctcgcctg tcgcatgcgt gacgagccct ccgcccgtct
     6961 ccgtgagcga gtatccgacc tcggcgtgct gcgcgaggtc gagcccggag atctcgtctt
     7021 cttccgtgac ccgcaatccg atcgttgcgt ccacgagctt ggcgatgatg taagtcacga
     7081 cgaaggcgaa cacgagcgtg acgccgaccg cgatcagttg cttgccgagg agagctaggc
     7141 ctccaccggc gatcagtccg tcggcgccga actcgttgac cgctgcggtc gagaagatac
     7201 cgaggaggag agcaccgacg atgcctccga ccatgtgcac accgaccacg tcgagcgagt
     7261 cgtcgtagcc gagcttgtac ttgagtccca cagcccacga gcagacgcaa ccggcgatga
     7321 ggccgatggc gatcccaccg agcggtccga cgaatccggc cgcaggcgtg atcgcaacaa
     7381 gaccggctac cgctccggaa gcaacaccca gggtcgtcgc cttgcgctct ttgcgagctt
     7441 ccatcagtgc ccagccgacg gctcccgccg cggcaccgag gttcgtcgcg aggaacgcgg
     7501 tcgcggcgag ctcccccgcg ccgagtgcgc tacctgcgtt gaagccaaac caaccgaacc
     7561 acaagatccc cgttccgagg atcgtcatcg tcaggttgtg aggaacgaaa gcttccttcc
     7621 cgaagccttt gcgcttgccg atcacgagga tcgcggcgag tgcggccaca ccggcgttga
     7681 tgtggacgac cgtgccgccg gcgaagtcca gggctccgag cttgaagagc caaccttcgg
     7741 ggtgccacac ccagtgggcc accggtgagt agacgaagac cagccacagc gccatgaacg
     7801 cgacgtagcc cgagaacttc atccgctcgg cgaaggcgcc ggttatcagt gccggcgtga
     7861 tgacggcgaa catcatctgg taggtcgcga acaggttgtg cgggatcgtc gccccgagcc
     7921 cgtcggggtg caccccgccc ccgacccccg agaatccgat gaagtcgagt ccgccgatca
     7981 gcccgcctac gtccggtccg aaggcgagcg tgtagccgat gaggacccac aggatcgaga
     8041 tgagtcccat cgcgaagatg ctgtgcatga gggtgccgag gacgttcttc gccctgacca
     8101 tccccccgta gaacaaagcg agccctggtg tcatgaagag caccaaggcc gttgaagcga
     8161 ggatccacgc ggtatcgccc gtatccagag tgatcacccc tttactcgta gatcgcctgc
     8221 ggcgggggcg gacgccggtg tcgccttccg caaccgattc cctgaacaac ccactcgtgg
     8281 ggaagtggct gcttgcaggt cactgtttat ggaagtggtg tttcggtttt gtttcggagc
     8341 cgtttcccgg cagtaaatgt tcgtccacag cccagaacgc ggcaaagaga ggggcctacg
     8401 cgccttaccc tggatctcgg tatcccggag gtctgcggag ggttatgaga tggcggaacg
     8461 cccggttcga gccgccttcc cggagttaag gcacgccgag gcggcggctc tcaacgcgac
     8521 cttgatcgac cgagccttgc ccgcggacca agctcgcgtt gcttcggtcg tccgcagtcc
     8581 tctcgacagt ggctggtacc gaaccgaggt cctggaggtg caatcggact ccggcgaggt
     8641 agcgaaggtc ttcctgaaag atttcggctc cgttgacaaa ggaaagagca ggatggagga
     8701 ccgtcgcgag cgggaacgct atttctacgg gcgggtcttc cccgacggct cgctcggtct
     8761 accccgctac ataggtgaga tctgggggcc ggatcggttc tggctattgc tcgagtacat
     8821 cgatgcggtg ccgctcaagt ggtgcgagct ggacgtgtgg ttccaggccg cggcgtggct
     8881 cggtcgattc cagaccatga ccgcccggaa gctagaggag ctagacgcgt cgggtcggtt
     8941 cactaagcac ggacccgagt acttcgagaa tgcttcgatc cgagcagtga agggatcggc
     9001 tcgattcgga gccgacgtcg tctccacggt cgaacgagcc gtgcccgtct atctcgagta
     9061 tgcggcaggt ctcatgagac ctccgcgcg
//
LOCUS       DATMED010000262         6644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5572297, whole genome shotgun sequence.
ACCESSION   DATMED010000262 DATMED010000000
VERSION     DATMED010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5572297"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VNP73_06720"
     CDS             <1..1031
                     /locus_tag="VNP73_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65650.1"
                     /translation="DQRAQDMNEVLKASKRGADLVRQLLAFSRKDVVNPVVLNLNNVV
                     LDLGKMLRRTLGEHIQLEMSLDPELWNVEVDPGHVEQILVNLAVNARDAMSGGGRLSI
                     RTSNNHGNPSSDPAGATRFAMLVIEDNGQGMTPEVTARIFEPFFTTKDVGSGTGLGLA
                     TVYGIVRHWHGLIDVDSEPGRGTTFQICLPATEDAIEEDEAAGPVEEDLRGKETVLVV
                     EDQTEVLEVVGRILRTNGYGVLRAASIDAASTLVELNASEIDLILADVVMPGGSGYDL
                     KESLSSSHPNLKVVLMSGFSGDWVNGNNGAGNNGAGKVEVQLRKPFSSTELLRLVRSE
                     LDGSELIA"
     gene            1063..1467
                     /locus_tag="VNP73_06725"
     CDS             1063..1467
                     /locus_tag="VNP73_06725"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWL65651.1"
                     /translation="MVVERIDATDVLGPSPGYAYAAAATEGVTVYTAGAVPVDPNGKL
                     LGPDDLKAQTQACIANLRTVLERAGAAPDMVVKTTIFVVGAKQSDLPEVWSEFARTPW
                     AAAPSTLVGVTYLGYSGQLVEIEAVAVVPSER"
     gene            1464..2567
                     /locus_tag="VNP73_06730"
     CDS             1464..2567
                     /locus_tag="VNP73_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWL65652.1"
                     /translation="MRSRRWSLPVALLGFLVLVAGSVPAVAQEDPCARDDGWATIRAQ
                     WVTEIVALTNSHRASIGLDQLAVSRSLTRAALWKASHMAAYEYMAHDDPAPPIERTWE
                     QRVDDCGYTESAGENIAYGYRTPEEMFQGWLDSPGHRRNIERAEFSAIGVGVATNVSG
                     ITYWTQVFGGEDDSANDVHSPPQPGADTIQGAEDSRLTFSPLTNDFDPDGDPIEIVGS
                     RGPDHGRLQISLPGEITYIPDRNFFGEDSFAYTVMDVFGLGAEATVSVSVLGVNDDPD
                     VAGEERRLRAGRSITVDVLSNDRDVDGDRLRLDRIVRGPRSGEIVNLDKRTGTIDYRA
                     AAGAAGKEDRIVYRVTDRNGGAARGTLRLKIRR"
     gene            2568..3452
                     /locus_tag="VNP73_06735"
     CDS             2568..3452
                     /locus_tag="VNP73_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWL65653.1"
                     /translation="MTRVAHVALALLVTVACTQGKETVAIPRSTPTPAPSVSPTQEDE
                     REPREREDTSDRWVTARPSWLGKRVLPKGPSGFGVAGETPPILRNRRFPTIDILPAPK
                     RERFAATIGPVPRSVLARSTWRPRCPVAESDLSYLTMTFWGLDHEAHTGEMIVHASVA
                     EEITGVFEALFHARFRIEEMRVVTLKEQRRWRTAPTGDTNVTSSFECRKATLGSSWSE
                     HSYGLAIDVNPFHNPYLRGDLVGPELARAYTDRTWERPGMIFEGDAVTRAFDAIGWGW
                     GGRWSSLKDWMHFSQSGH"
     gene            complement(3489..6128)
                     /locus_tag="VNP73_06740"
     CDS             complement(3489..6128)
                     /locus_tag="VNP73_06740"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWL65654.1"
                     /translation="MKLLRAALAAFLTTALASSALAQGQSPVQPYGAGDGGVTVLNVL
                     PPGQGEYQNGPEQAAGQQSPHNSDQIAMYEKLVQGAPDVTAASLTEYFKDATFGVKPE
                     DVATEYSPRDGVVIQRDTGFGVPHIYGTTRQDVMFGAGYASAEDRLFMMDTLRHFGRG
                     RLSEFLGASEANLASDRAVYLNAGYTEEEFQAMIDRVKGLDPELGAIAFDDVTAYVEG
                     INQYIAEARTDPSKLPAEYEALQLVPEDWKPTDTVAVASLIGSQLGVGGGGELDNAAF
                     VNALQKEGFSFKQSRKILADFNFPDDPESPSTTDKRFVWNTDLGKPNKASRAIPDDAA
                     EVARRARRLASFPGYVDAPWGRIPLSFPDAMSNALLIGSDLSASGRPLAVMGPQTAYW
                     SPEILMELDMHGPGIDARGVGFPGISLYVLLGRGTDYAWSATSAGGDQVDTFVEELCD
                     PGGGEATVDSTGYMEGGKCVEMYSRTDSWQAKPTAASVPPQTVAVEMTTERTDDGIVQ
                     ARGTVGGKPVAFTALRASFMKEVDSALTYVEMSDPERVRNAKDFQKAFSRFTFTFNWF
                     YVDDKDIAFQLGGTHPLRARHTDPDLPMWGNGKWDWRGNLSFKKTPKDINPARGWMTS
                     WNNKQAPGFRANDGNFSFGPVQRVQMLDDGIKRYKKQDGKISLVDAVNIMGEAATTDL
                     RGTRVVPWMLKVIGKPKDAKLKGAVGLLKEWTKAGSHRRDKDGDGTYENSSAIALMDQ
                     WWTLAVDAIFKPALGAAWDVIPSGYHDAPRAQGSAFQSGFYSHVNKDLRTILGRRVRG
                     RFSRIYCGKGSVKKCRQALTDSLAAAVKALEEQFGADPASWDADEVGDQIDFTPVGLQ
                     DQPNMPWQNRPTFQQVLEFEPAP"
     gene            6201..>6644
                     /locus_tag="VNP73_06745"
     CDS             6201..>6644
                     /locus_tag="VNP73_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65655.1"
                     /translation="MPSESSDRLSVSLLCVELDYRGDLEANPREQKALLADIARVAGR
                     HGGRDGELQGSVFRVVFSVPFSRMPARDAVAAAESLKAVLHRGPLQVTWRVAIATATS
                     IAEGSVVYGDAMRIAKELLDRADEGAIEIDPETARLVASSTVGGRR"
BASE COUNT         1188 a         2092 c         2125 g         1239 t
ORIGIN      
        1 aagatcagcg cgcccaggat atgaacgagg tgttgaaggc aagcaagcgt ggcgccgatc
       61 tcgtacgcca gctgctcgct ttctcgcgca aggacgtggt caaccccgtc gtgctcaacc
      121 tcaacaacgt ggttctcgat ctcgggaaga tgctgcgccg aacgctgggc gagcacatcc
      181 agctggagat gagcctcgat ccagagctgt ggaacgtcga agtcgatccc ggtcacgtcg
      241 aacagatcct cgtgaacctc gccgtgaacg cgcgggatgc gatgtcgggt gggggacgct
      301 tgtcgatccg cacctccaac aaccacggaa atccgtcgag cgaccccgcc ggagcgactc
      361 gtttcgcgat gttggtgatc gaggacaatg gtcaggggat gaccccggag gtcaccgctc
      421 ggatattcga gccgttcttc accacgaaag atgtcgggtc ggggaccggt ctcgggctcg
      481 cgaccgttta cgggatcgtg aggcactggc acggcctgat cgacgtggac tcggagcccg
      541 ggcgcggaac cacgttccag atctgcctgc ctgcgaccga ggacgcgatc gaagaggacg
      601 aggctgccgg cccggtggaa gaggacctgc gggggaaaga aaccgttcta gtggtcgagg
      661 atcagaccga ggtgctcgag gtggtcggac ggatcctgag aacgaacgga tatggagtcc
      721 tccgggcagc ctcgatcgac gccgcaagca cgctggtcga gctgaacgcg tcggagatcg
      781 acctgatcct ggccgatgtg gtgatgccgg gcggatccgg ttacgacctg aaggaaagcc
      841 tgtcgtcgtc gcacccgaac ctgaaggtcg tgctgatgtc gggcttcagc ggagactggg
      901 ttaacggcaa caacggagcc ggcaacaacg gagccggcaa ggtcgaggtc cagctccgca
      961 agcccttcag ctcgaccgaa ctgctccgat tggttcggag cgagctcgac ggcagtgagc
     1021 tgatcgcctg acgtagtcac cgcgtcgcgg tatacagcgt tgatggtcgt cgaacgcatc
     1081 gatgccaccg atgtcctcgg tccctcaccc ggctacgcct atgcggccgc ggcaactgaa
     1141 ggtgtgaccg tgtacaccgc gggggccgtg cccgttgatc ccaatgggaa actcctcggt
     1201 ccggacgacc tcaaagccca gacgcaagcg tgcatcgcga atcttcggac cgtcctggaa
     1261 cgggccggtg cggcaccgga catggtcgtc aagaccacga tcttcgtcgt cggcgccaaa
     1321 caatccgacc tcccggaagt atggagcgag ttcgcacgaa ccccatgggc ggctgctccc
     1381 agcacgctcg tcggcgtcac ctacctcggc tattcgggac aactggtcga gatcgaagcc
     1441 gttgcggtgg ttccctcaga acgatgagat cccgacgctg gagccttccg gtcgctcttc
     1501 tcgggttcct ggttctcgtt gcgggttcgg ttccggcggt tgcccaggaa gacccgtgcg
     1561 cgagggacga tggatgggcg acgatccgtg cgcagtgggt taccgagatc gtcgctttga
     1621 cgaactcaca tcgggcatcc atcggcctcg accagcttgc cgtgtcgcgg tcgctgacgc
     1681 gcgcagcatt gtggaaagcg agtcacatgg ccgcatacga gtacatggct cacgacgacc
     1741 ctgctcctcc catcgagcgc acgtgggagc aacgagtcga cgactgtgga tatacggaga
     1801 gcgcggggga gaacatcgcc tacggatacc ggacgccgga ggagatgttc cagggctggc
     1861 tcgactcgcc cgggcaccgg agaaacatcg agcgtgcaga gttctcggcc atcggtgtgg
     1921 gtgtggcgac caacgtttcg ggcatcacct actggacgca ggtcttcggc ggtgaggacg
     1981 actccgcgaa cgacgtgcac agccctcccc aacccggagc cgacaccatc caaggagccg
     2041 aggattcccg cctgaccttc tcgccgctca caaacgactt cgatcccgac ggagatccga
     2101 tcgagatcgt gggttcccgt ggccccgacc acggcaggct gcagatctcc ttgccgggcg
     2161 agattacgta catccccgac cggaacttct tcggtgaaga ctcattcgcc tacacggtga
     2221 tggacgtgtt cgggctggga gccgaagcga ccgtctcggt ttcggttctg ggggtcaacg
     2281 atgatcccga tgtcgcagga gaggagaggc ggctgcgcgc aggtcgatcg ataaccgtgg
     2341 atgttctttc caacgatcgc gatgtcgacg gcgaccgcct gcgcctcgat cggatcgtga
     2401 gggggccgcg ctcgggcgag atcgtaaacc tggataagag aaccggaacg atcgactacc
     2461 gggccgcagc cggagcagcg ggcaaggaag atcgcatcgt ttaccgcgtg accgaccgca
     2521 acggcggtgc cgcacgcggg acgctgcgtc tgaagatccg ccggtagatg acacgcgtag
     2581 cgcacgtagc actggctctg cttgtgaccg ttgcgtgcac gcagggcaag gagacggtgg
     2641 cgatcccgcg ttcgacccca accccggcac catcggtgtc acctacccaa gaggacgagc
     2701 gtgagccgag ggaacgcgag gacacctccg accgctgggt gacggcccga ccgtcatggc
     2761 tggggaagag ggtgctgccg aagggtccca gtggtttcgg tgtggccgga gagacacctc
     2821 ccatcctccg taaccggcgc ttcccgacga tcgacatctt gcccgcgccg aagcgtgagc
     2881 gattcgccgc cacgatcggg ccggtgccgc gttccgttct cgcgcgctcg acgtggagac
     2941 ctagatgtcc cgtggccgaa tcggatctgt cttatctgac tatgacgttc tggggtttgg
     3001 atcacgaggc tcacaccggt gagatgatcg tccacgcttc cgttgccgag gagataacgg
     3061 gggtattcga ggctctcttc cacgcacgat tccgcatcga ggagatgcgc gtagtgactc
     3121 tgaaggagca gcggaggtgg cggaccgctc ccacgggcga tacgaacgtg acctcgtcct
     3181 tcgagtgcag gaaggcgacc ctgggcagtt cgtggtccga gcattcatac gggctggcga
     3241 tcgacgtgaa cccgttccac aacccatacc tgcgcggcga tctggtcggc cccgagctgg
     3301 cgcgcgccta caccgaccgg acgtgggagc ggcccgggat gatctttgaa ggcgatgcgg
     3361 tcacgcgtgc gttcgatgcc atcgggtggg gctggggcgg tcgctggagc tcgctcaagg
     3421 attggatgca cttctcccag tctgggcact agtgatggcc gaggccatca ctaggcgggc
     3481 actgaccttt agggcgcggg ttcgaactcc aagacctgtt ggaacgtggg ccgattctgc
     3541 cacggcatgt tcggctgatc ctgaagaccg acgggggtga aatcgatctg atccccgacc
     3601 tcgtcagcgt cccacgaagc agggtctgct ccgaactgct cctcgagggc tttgacggct
     3661 gccgcgagac tgtcggtgag tgcttggcgg cacttcttca ccgatccctt cccgcaatag
     3721 atgcgcgaga accgaccgcg tacccgtcgt cccaggatcg tcctgaggtc cttgttgacg
     3781 tggctataga acccgctctg gaacgcgctt ccttgagccc tcggagcatc gtggtagcca
     3841 ctggggatga cgtcccaggc tgcgccgagt gcgggcttga agatcgcgtc gaccgccagt
     3901 gtccaccact gatccatcaa cgcgatcgcc gaggaattct cgtacgtccc gtctccgtcc
     3961 ttatcgcgcc ggtgcgagcc ggccttcgtc cactctttca gcagccccac cgcacctttc
     4021 aacttggcgt ctttgggctt gccgatcact ttcaacatcc acgggaccac gcgcgtacct
     4081 cgcagatcgg tcgtggccgc ttcgcccatg atgttcacgg cgtcgaccag actgatcttc
     4141 ccgtcctgct tcttgtagcg cttgatgccg tcgtccagca tctgcacgcg ttgcaccggc
     4201 ccgaacgaga agttgccgtc gttcgctctg aatccggggg cctgcttgtt attccacgag
     4261 gtcatccacc cccgcgcggg gttgatgtct ttcggcgtct tcttgaacga gaggttcccg
     4321 cgccaatccc acttgccgtt cccccacatc gggaggtccg gatcggtgtg acgcgctctc
     4381 agaggatgtg tcccgccgag ctggaaggcg atgtccttgt catcgacgta gaaccagttg
     4441 aaggtgaagg tgaaccgtga gaacgccttc tggaaatcct tcgcattcct gacgcgctcg
     4501 gggtcggaca tctcgacgta agtaagtgcc gaatcgacct ccttcatgaa ggaggcacgc
     4561 agggccgtga atgcgaccgg cttaccgccg acggtgccgc gggcctgcac gatcccgtcg
     4621 tcggttcgct ccgtcgtcat ctctacggcg actgtttgag ggggcacgga cgccgccgtg
     4681 ggtttcgctt gccaactatc cgtgcgcgag tacatctcga cgcacttccc gccctccatg
     4741 tagccggtgc tgtcgaccgt tgcctcaccg cctccgggat cgcatagctc ctccacgaac
     4801 gtgtcgacct ggtcaccacc agcagaggtt gcactccacg catagtcggt ccctcgtcct
     4861 agaaggacgt acagcgatat ccctggaaag ccaacgccgc gcgcatcgat cccgggtccg
     4921 tgcatgtcca gctccatcag gatctcgggc gaccagtacg cggtttgcgg tcccatcacc
     4981 gcgagcggac gacctgaagc agacaggtcc gacccgatca agagagcgtt gctcatcgca
     5041 tcggggaacg acagaggtat gcgcccccat ggggcatcga cgtacccggg gaaggaggca
     5101 agtcgccgag cgcgcctggc gacctctgca gcatcgtcgg gtatggcccg ggaggccttg
     5161 ttcggcttgc ccagatccgt gttccagacg aaccgcttgt cggtcgtcga tggtgactcg
     5221 gggtcgtcgg ggaagttgaa gtcggcgagg atcttgcgcg attgcttgaa actgaaaccc
     5281 tctttctgga gagcgttcac gaaggctgcg ttgtccagct ctcctcctcc gccaaccccc
     5341 agctgagatc cgatcaagct tgcaactgcc acggtgtccg tcggcttcca gtcttccggc
     5401 acgagctgca acgcctcgta ttcggccggc aacttggaag ggtcggtgcg ggcttcggca
     5461 atgtactgat tgatgccctc gacgtacgcg gtgacatcgt cgaacgcgat cgctcccagc
     5521 tcggggtcga gacccttgac ccgatcgatc atcgcttgga actcttcctc ggtgtagccc
     5581 gcgttcaggt agacggcccg gtcggaggca aggttggcct cgctcgcgcc caggaactcg
     5641 ctcagccggc cccggccgaa gtgacgaaga gtgtccatca tgaataggcg gtcttccgcc
     5701 gacgcgtagc cggcgccgaa catgacgtcc tggcgggtag ttccgtagat gtgtgggacg
     5761 ccgaatcccg tatcgcgttg gatcacgaca ccgtcccgag gtgagtattc ggttgccacg
     5821 tcctcgggct tcacaccgaa ggtcgcatcc ttgaagtact cggtcaagct cgccgcggtt
     5881 acgtcggggg ccccttgcac gagcttctcg tacatcgcga tctgatcgga gttgtgaggg
     5941 ctctgctggc ccgcggcttg ctcgggaccg ttctgatact ccccctggcc cggcggaagc
     6001 acgttcagca cggtcacgcc gccgtctccg gcaccgtagg gctgcaccgg ggactgcccc
     6061 tgtgcgagag cagacgacgc aagtgccgtc gttagaaaag cagcgagcgc ggctctaaga
     6121 agcttcacgg ttcgtcctcc cacctgggca ccagccgggg atcccccctc actctattgc
     6181 cgcctctaga atcgccgcgc gtgccttccg aatcttcaga tcgcctgtcc gtttccctcc
     6241 tttgcgtaga gctggactac aggggcgacc tcgaagcaaa tccccgcgaa cagaaggcgc
     6301 ttctggccga catcgctcgg gtggccgggc gccacggggg ccgcgacgga gagctgcaag
     6361 ggagcgtgtt cagggtcgtc ttctccgtcc ccttcagtcg catgccggcc cgcgacgccg
     6421 tggccgcggc ggagtcgttg aaggccgtct tacatcgcgg cccccttcag gtgacctggc
     6481 gtgtcgcgat cgctacggcc acaagcatcg ccgaggggtc ggtcgtctac ggggacgcga
     6541 tgaggatcgc gaaggaacta ctcgatcgag ccgacgaggg agcgatcgag atcgatccgg
     6601 agaccgcccg acttgttgct tcatcgacgg tgggcggccg ccgc
//
LOCUS       DATMED010000263         3569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2141701, whole genome shotgun sequence.
ACCESSION   DATMED010000263 DATMED010000000
VERSION     DATMED010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141701"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VNP73_06750"
     CDS             <1..526
                     /locus_tag="VNP73_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65656.1"
                     /translation="LNKVGVFMRDSTVPGPHAAGPSQSVQQKGRSNTPSCTGGSTLSV
                     LSPVGGVRIGTPGIAQGQPGFVPGDRGDALVMMQAPGEIFASISLTTKDYLSRARNVM
                     VFGMANDHIGYIIPAEQYDVRGANAVGIAQPSLEMTNYEESLSTGRCTGDQIQNALVE
                     IGTNLGVLGPGESR"
     gene            753..2915
                     /locus_tag="VNP73_06755"
     CDS             753..2915
                     /locus_tag="VNP73_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65657.1"
                     /translation="MKFSRRVQRALLATIVFVLTLALVQLAPSGGYGIPAFGQVPTPT
                     PGAPTIQFINPSAGTSLEVSNKDDGSGDKTIHLVAWVSAIPENPVVEFKYKSNQSGSQ
                     EIPIGDGVAQVGPGDTFETNWTLGTAGPEGPDEPGTTITRGTSGVVKVILSSGSTEVS
                     RDEETIRINEQTPPACPPPGPNNTACEDEAQANNVEMTYPTNGGPFGHYQPTTDQGPV
                     NSLIDVTSSAETVNINAYYTLSRPGTEPSWKQCKAGTGAVNETRANTLDGIKCEYDPN
                     DNPTRVTAVAVRATDAHTSAPGVVESVNVDGADAHRVEPYLQDPESIVLLPPTQEFES
                     GKCAPVITARVMDQANPPRKVGDVNVDVHAMGPSDALSFDDAGGTYSTTNKAPENHPS
                     ESAVACEQATPGFSGAQGDHESAAEDDIKHIESLDDTNDAGEWKFALFSPDEGGTQYS
                     VWADEDGNDRRCSEEAVENGSIGWDTSAPGPTDFAPEESSCPKPAPSGSPPPSATPTS
                     SPSSSPSAIPSPGQRTLTLAAETTEATAGDTIKLSGQLFAPNNPTCVDNEFISITQRP
                     HGARSAEPLTTASTDASGRYTAEITVRSSADYQAAAPAHDTCGEAFSSPVSSLVKVAI
                     SIRASNRTPTRGKFFNIVVRVAPNHRGTQVALFRKIFGRWIKDEVKKLNDSSRAVFKV
                     SATFKRQSFRAKWRSQDEEHVGNTSAPIKVRPRKKARS"
     gene            2923..>3569
                     /locus_tag="VNP73_06760"
     CDS             2923..>3569
                     /locus_tag="VNP73_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65658.1"
                     /translation="MKKPILAVLALMAGSLPMVAGAQTQVTQIGFLNPSPYVDAQIPE
                     LSDEEGRSVHLVAWVAPVPASPGVEFELQPNTASNDGGSSTTIQASRVGEGDTWEARF
                     SIPAAFQEGEYILRARVFSATEVVATTEITVKLDHASPPPELETVDLIRPVNGGAMGF
                     YLPPGKGANSAVRVAASRGTERVRVLYTLTPPGTAPVWTHCGSGVPDPETQIAAV"
BASE COUNT          746 a         1156 c         1100 g          567 t
ORIGIN      
        1 cttgaacaag gttggagtct tcatgcggga ctcgaccgtc cccggccccc acgcggccgg
       61 accctcgcaa agcgttcagc agaaaggccg gtccaatact ccgagttgca ccgggggtag
      121 caccttgagc gtcctctccc cggtcggcgg cgttcggatc ggcacgccgg gcatcgcaca
      181 aggtcagcca ggcttcgttc ccggtgaccg cggcgacgcg ctcgtgatga tgcaggcccc
      241 gggcgagatc ttcgcctcga taagtctcac aacgaaggac tatctgtcgc gtgctcgcaa
      301 cgtgatggtt ttcggcatgg ccaacgacca catcggttac atcatcccgg ccgagcagta
      361 cgacgtgcgt ggagccaacg ccgttggtat cgcccaaccc tcgctcgaga tgacgaacta
      421 cgaggaatcg ctgagcaccg gccgctgcac cggcgatcag atccagaacg cgctggtcga
      481 gataggcacc aacctcggcg tcctcggccc gggcgagagc cgctaagcgg ctaacccaca
      541 gatagcggtc cgaaaaaacc tctagaagtt tcgttccttg tggagttaca tgtcctacct
      601 gtgcaacggg atgctcggca gcggcgctta gttcacccga aagagtgaag gaagtcagcc
      661 tcttcacgcg gaacagtgac gattccggac aaatcgactg ggtggcttaa cgcgggcagg
      721 aggttggtgg cgtgagcacg attggcggcg caatgaaatt cagcagaagg gtccaacgtg
      781 ctctgttggc gacgatcgtg ttcgtactca ccctcgctct agtccaactg gccccctcgg
      841 gcggttacgg gatccctgca ttcggccagg ttccgacgcc gacgccggga gcccccacga
      901 tccagttcat caacccctcc gccggcacga gcctggaggt gtcgaacaaa gacgacggca
      961 gtggggacaa gaccatccac ttggtggcct gggtcagcgc catccccgag aatccagtgg
     1021 tcgagttcaa atacaagtcg aaccagtccg gatcccaaga gataccgatc ggggacggtg
     1081 ttgcacaggt cgggcccggt gacacgttcg agaccaactg gaccctcgga acggccgggc
     1141 cggaagggcc cgacgaaccc gggacgacta tcacacgggg aaccagcggc gtagtgaagg
     1201 tcatcctcag cagcgggagc acagaggtct ctcgcgatga agagaccatc cgaatcaacg
     1261 agcagacgcc accggcttgt ccacctcccg ggccgaacaa cacggcgtgt gaggacgagg
     1321 cccaggccaa taacgtcgag atgacatatc ccaccaacgg tggaccgttc ggtcactacc
     1381 agcccaccac cgaccaagga cccgtcaaca gcttgatcga cgtcacttcc agtgcggaga
     1441 ccgtgaatat caacgcctat tacacgctgt cgagacccgg taccgagccc tcgtggaaac
     1501 agtgcaaggc ggggacgggg gcggttaacg agacgagggc caacacgctc gacggcatca
     1561 agtgcgagta cgacccgaat gacaatccga cgcgggtgac ggccgtagcc gtccgggcca
     1621 ccgacgcgca cacttctgcc cccggggtgg tcgagtccgt caacgtcgac ggcgccgacg
     1681 ctcatcgagt ggagccctac ctgcaggacc ccgagagcat cgtgctcctt ccgccgacgc
     1741 aagaattcga gtcgggcaag tgcgccccag tcatcacggc cagggttatg gaccaggcga
     1801 atcctccccg caaggtcggc gacgtcaacg tcgacgtaca tgcgatgggt cccagcgacg
     1861 ccctttcctt cgatgacgcc ggcggcacct actcgactac caacaaggcg ccggagaacc
     1921 atcccagcga gtcggccgtc gcgtgcgagc aggccactcc gggcttcagc ggtgcccagg
     1981 gtgaccatga gtcggccgcc gaagatgaca tcaaacacat cgagtcgctg gacgacacga
     2041 acgatgcggg tgagtggaag ttcgctctgt tcagtcccga cgaggggggg acccagtact
     2101 ccgtatgggc agatgaagac ggcaacgatc gtcgttgctc cgaggaagca gtagagaacg
     2161 gatcgatcgg ctgggacacg agcgcacccg gtccgaccga cttcgctccc gaggagtcga
     2221 gctgtcccaa gccggccccc tccggctcgc ctccgccctc ggctactccg acctcatctc
     2281 catcgagtag cccgtccgcg atcccgagcc ccggccagcg gacgctgacg ctcgcggccg
     2341 agaccacgga agcaacggca ggagacacca tcaagctctc gggccagctg ttcgcgccga
     2401 acaatccaac ctgcgtcgat aacgaattca tctcgatcac acagcggccg cacggagcac
     2461 gttcggccga gcctttgacg actgctagta cggacgccag cggccgatac accgcagaga
     2521 tcaccgtgag gtcgagcgcc gactatcagg cggcggcgcc ggcccacgac acttgtggcg
     2581 aggcgttctc ctctcccgtg tcatctctgg tcaaggtggc gatctcgatc cgcgcaagta
     2641 atcgcactcc gacccgagga aagttcttca acatcgtcgt gagggtcgcg ccgaaccacc
     2701 gaggcaccca ggttgcgctg ttccggaaga tcttcggccg ctggatcaag gatgaggtga
     2761 agaagctcaa cgatagttcg agggccgtgt tcaaggtcag cgcgactttc aagcggcaat
     2821 ccttccgggc taagtggaga agccaagacg aggagcacgt cggaaatacc agtgctccca
     2881 taaaggtcag accgcgcaag aaggcgcgtt cttagcaccg tcgtgaagaa gccgatcctg
     2941 gcggtgctcg ctctaatggc gggctcgctc cctatggtgg ccggcgcgca gactcaagta
     3001 acccagatcg gtttcctcaa cccgtcgccc tacgtcgacg cgcaaatccc cgagctttcc
     3061 gatgaagaag gcagatcggt gcatctggtc gcctgggtcg ctccggtccc ggcttcgccg
     3121 ggcgtcgagt tcgagttgca accaaacacc gcgtcgaacg acggaggaag tagcaccacg
     3181 atccaggcga gtcgggtcgg tgagggagac acatgggagg ccagattctc tatcccggcc
     3241 gctttccagg agggcgagta catccttcgg gctcgagtct tctcggcgac cgaggtggtc
     3301 gccacgaccg agatcacggt gaagctcgac catgcgagcc ccccgccgga actcgaaacg
     3361 gtcgatctca tccgccccgt gaatggtgga gcgatgggct tctaccttcc accgggcaaa
     3421 ggggcgaaca gcgccgtgcg ggtcgccgcg agtcgcggca ccgaacgggt acgcgtcctc
     3481 tacacgctca ccccgccggg aacggctccg gtctggaccc attgcggcag cggcgttccg
     3541 gatcccgaga cgcagatagc cgccgtgag
//
LOCUS       DATMED010000264         4101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3283909, whole genome shotgun sequence.
ACCESSION   DATMED010000264 DATMED010000000
VERSION     DATMED010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283909"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VNP73_06765"
     CDS             complement(<1..478)
                     /locus_tag="VNP73_06765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWL65659.1"
                     /translation="MRYVALGDSLTEGVGDPIADGSLRGWADMLAGALKRSNPDLDYR
                     NLARRSLTAAQVRQSQLEPALAVAPDLASVAVGMNDVLAPSFDKNSFERDLVSLVHPL
                     IESGATVLVGTLPEDLPLLRLMRGKAAATRSRLKTVGAVVGAVCGDLGALVVDAPEE"
     gene            552..869
                     /locus_tag="VNP73_06770"
     CDS             552..869
                     /locus_tag="VNP73_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWL65660.1"
                     /translation="MAATGSVYTSGDWIVTAGREEEFVKAWTEFVDWAMATVPGARFA
                     TLIQQKDDPCHFISFGEWDGEKQVAAWRSHPEFPQKLIAPRSLCEQFVGKDFEVAASI
                     EAT"
     gene            complement(866..1726)
                     /locus_tag="VNP73_06775"
     CDS             complement(866..1726)
                     /locus_tag="VNP73_06775"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWL65661.1"
                     /translation="MPIFESLKELDNVIAPALIVSFEDWVDAGQSGSSAARHIAEGGE
                     VVATADPDGIYDYRSHRPVLDIVDGAPKHFAWPEMTLTRRRVSERDLFVLSGPEPDYR
                     WKEFSAEVLEIALRIGIVEHVSLGAIPSAVPHTAPTQVMMTASSNELLRGSTPAIQGL
                     LRVPAAAVSLVEWALAEAGIPTVGFWAQVPHYASPFAAGAIALIRRVEDHLGVTIGAG
                     SLEEEVSSQRENLEKIFAANPEATEYLERLQGLVGEEPVPPAENIAAEVERFLRNQRR
                     GDPGPNPFSP"
     gene            1758..2192
                     /locus_tag="VNP73_06780"
     CDS             1758..2192
                     /locus_tag="VNP73_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWL65662.1"
                     /translation="MSDDDKMNVGTVIKLLNKAIPLVFRSASLYTWAGGAAVGVEFQA
                     VGKTFEEFGHLELDDARRLVEKLVALGGDATTEVAPFDAVPSVAGLTKIIEYETEALE
                     ALREVIPETGQEARSEALEHLLEHIIMRKQEQVDFLERVRRG"
     gene            complement(2189..3286)
                     /locus_tag="VNP73_06785"
     CDS             complement(2189..3286)
                     /locus_tag="VNP73_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWL65663.1"
                     /translation="MPVARIVLCACLALLTFAQIGRAQPQPDPLDELSLKEKVGQLVM
                     FSVRGQGLTNGERAAIQRHHLGAVILFANNYSNPTQLARLTEQIQRTARRANPLRIGA
                     LISVDQEGGIVKRFPDMPPRYSAPEMGRINRKALTYNQGRDTGGALHRAGVNVDLAPV
                     ADLDLAPEHVMRSRSFGSNRYRAARLVKAFSKGLQSRKVAATLKHFPGLGGGTRNTDF
                     GKSYVRRTRSQLRNIDAVPFHRAIDGGARLVMVSHAIYPHDGGSRPASLNPFITKRRL
                     RDGFDFTGVAISDALGAVAWKFGGSTIKACAGTIRAGVDIALITGDTSVAGACARKIR
                     RQVRNNRISQHRIDQAVERVLELKVWLGLTP"
     gene            3670..>4101
                     /locus_tag="VNP73_06790"
     CDS             3670..>4101
                     /locus_tag="VNP73_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWL65664.1"
                     /translation="MSSFAELGASAPVARALERRNITQAFPIQAMVIKDATAGKDVLA
                     KSRTGSGKTLAFAVPMVDRIDQGAKRPAGLILVPTRELAVQVTDEMKDIAAAQNLRVA
                     PVYGGVGLEKQAERARKAHIVVATPGRLTDLVNRRLLSLDNI"
BASE COUNT          788 a         1272 c         1295 g          746 t
ORIGIN      
        1 attcctcggg cgcgtcgacg acgagggcac cgagatcgcc acatacggcc ccgactacgg
       61 cacccacggt cttgagccgg ctgcgcgtcg cggccgcctt ccctcgcatc agccgcaaca
      121 gcgggagatc ctccggcaag gtccccacca gcaccgttgc cccactctcg ataaggggat
      181 gtacgagcga tacgaggtct cgttcgaacg agttcttgtc gaagctcgga gcgagaacgt
      241 cgttcatccc cacggcgaca ctggcgaggt cgggggccac agccaacgcc ggctcgagtt
      301 gggactgtcg cacctgagcc gcggtcagcg acctgcgggc gaggttgcgg tagtcgaggt
      361 cgggattcga tcgcttcaga gcgccggcca gcatgtcggc ccaaccgcgg agggatccat
      421 cggcgatggg atcgcccaca ccctcagtca aactgtcacc caaagccacg tagcgcatcc
      481 aaggcaggct tgtcatcatg ggattgtgcc tcaagtcctg gggccggccg agcagtaaag
      541 gggggcaagt catggctgct acgggatccg tgtacacatc gggtgactgg atcgtcaccg
      601 ccggaagaga agaagagttc gtcaaggcgt ggacggagtt cgtcgactgg gcaatggcga
      661 ctgtccccgg agcacgcttc gcgacgctga tccagcagaa ggatgacccc tgccacttca
      721 tctcgttcgg agaatgggac ggcgagaaac aggtagccgc gtggcgcagc caccccgagt
      781 tccctcagaa gctcattgcc ccgcgctcgt tgtgcgaaca gttcgtcgga aaggatttcg
      841 aagtagcggc gtcgatcgaa gcaacctagg ggctgaaggg gttcggacca ggatctcccc
      901 tgcgctggtt cctcaagaaa cgctcgacct cggcggcgat gttctcggcc gggggcactg
      961 gctcttctcc gacaaggccc tgcaaccgtt ccagatactc ggtcgcctcc ggattggccg
     1021 cgaagatctt ctcgagattt tccctctggc tcgagacttc ctcttcgagc gagcccgcgc
     1081 cgatcgtcac gccgaggtga tcctcgaccc gacggataag cgctatggca ccggccgcga
     1141 acggggatgc gtagtgcgga acctgggccc agaagccgac ggtcgggatc ccggcttcgg
     1201 caagcgccca ctcgacgaga ctcaccgctg cggcgggtac acggagcaat ccctggatcg
     1261 ctggagtgct ccctcgcaac agctcgttcg aggatgcggt catcatcact tgcgtcggcg
     1321 ccgtgtgcgg aacggcggaa gggatcgcgc cgaggctgac gtgctcgact atcccgatcc
     1381 gtagcgcgat ctcgagtact tcggcggaga actccttcca gcgatagtcg ggctcggggc
     1441 cggagaggac gaacaggtct cgctcggaaa ccctccgcct cgtcaaagtc atctcgggcc
     1501 acgcgaagtg cttgggcgcc ccgtccacga tgtcgagaac cggcctatgg ctccggtagt
     1561 cgtagatccc atcggggtcc gcagtagcaa ccacttcgcc cccttcggcg atgtggcggg
     1621 cggccgaaga gccagactgt ccggcgtcca cccagtcctc gaacgaaacg atcagagcgg
     1681 gtgcgatcac gttgtccagt tctttaagac tttcgaagat gggcatacct caagcctagc
     1741 gagatgagag gagcactgtg tccgacgacg acaagatgaa tgttggcacc gtcatcaagc
     1801 tgttaaacaa agcgatccca ctggtattcc gctcggcctc cctctacacg tgggccgggg
     1861 gggccgccgt aggagtcgaa ttccaagccg tcggtaagac gttcgaagag ttcgggcatc
     1921 tcgagttgga cgatgcgcgc agactggtcg agaaactcgt tgccctcggg ggcgacgcga
     1981 ctacggaagt agcgcccttc gatgcggttc cgtccgtggc cggcctgacg aagatcatcg
     2041 aatacgaaac ggaggctctg gaggctctgc gcgaagtcat ccccgagacg ggacaggaag
     2101 ctcgctctga agcactggaa catctgctcg agcacatcat catgcgcaag caggagcagg
     2161 tggacttcct cgagcgcgtc cggaggggct agggcgtcag gccgagccag accttcagct
     2221 ccagcacacg ttcgaccgct tggtcgatcc tgtgttggga gatgcggttg ttgcgcactt
     2281 ggcgtctgat cttcctggcg caggcgccag cgaccgaggt gtcgcccgtg atcaacgcga
     2341 tgtccacacc cgcccggatc gtgcccgcac atgccttgat cgtgctcccg ccgaacttcc
     2401 acgcaacggc tcctagagcg tcgctgatcg caactccggt gaaatcgaat ccatcccgaa
     2461 gcctgcgttt ggtgatgaat gggttgaggc tggccggccg gctgcccccg tcgtgcgggt
     2521 agatcgcatg cgacaccatc accaagcggg ctcccccgtc gatcgccctg tggaagggaa
     2581 cggcgtcgat gttgcgtaac tgcgagcgcg tccggcgtac gtaggacttc ccgaagtcgg
     2641 tattcctggt gcctcccccg agtccgggga aatgtttgag cgtcgcggcc accttgcggc
     2701 tctggagtcc tttcgagaaa gctttcacca accgtgcggc acggtatcga ttcgatccga
     2761 agctgcgcga gcgcatcacg tgctcgggag ccagatcgag atcggccacg ggcgccagat
     2821 cgacgttcac acccgcacgg tgcaaggcac cgcccgtgtc ccggccctgg ttgtaggtca
     2881 gagccttgcg gttgatgcga cccatctcag gcgcggagta gcgcggcggc atgtcgggga
     2941 agcgcttgac gatcccaccc tcttggtcga ccgagatgag tgccccgata cgcagagggt
     3001 tcgcgcgccg cgccgtgcgc tggatctgtt cggtcagacg agcgagctgc gtcgggttcg
     3061 aatagttgtt ggcaaagagg atcaccgctc cgagatgatg gcgctggatc gcagcccgct
     3121 cgccatttgt gaggccctgc ccccgcaccg agaacatcac gagttggccc accttctctt
     3181 tcagtgaaag ctcatcgaga ggatcgggtt gaggttgggc tcgtccgatc tgagcgaaag
     3241 taaggagagc gaggcatgcg cacaggacga tgcgggctac cggcacgccg agatgctaag
     3301 accttggtgt gcccagaccc ttctccagca aggcgagatc tcgctgcccc gctacgggca
     3361 aacagcgcta ttccacctat ttcgaggcgc gggatgcagc ggtcgatatc aggcgttcca
     3421 cgggcgaaga tcacggcaag ccctaccgct gcgagcgctg tggagacttc catctgggta
     3481 aggcgagccc ctagatatgc cgaagaacag gaataagtcg tgtaacgtcg aacgttgtaa
     3541 gagataagca ccacttaggg cgtagatcgc ccgtgagatc tccttccggt tcctcctttc
     3601 gagcaagcgg gttggtcgat ctcccgttaa tcggtagcgg ttctaccgaa gtttgaagga
     3661 gttgaatctg tgtcttcttt cgcagaacta ggcgcgtccg cgccggttgc acgcgctctc
     3721 gagcgccgca acatcaccca ggcgttcccc atccaagcga tggtgatcaa ggacgctacg
     3781 gccgggaaag acgtgctggc caaatcccga acgggatccg gtaagaccct ggcgttcgcg
     3841 gttcccatgg ttgatcgcat cgatcagggg gccaagcgac ccgccggcct catcctcgtt
     3901 cccacgcgtg aacttgccgt tcaggtgacc gacgagatga aagacatcgc cgcggcccag
     3961 aacttgcgtg tcgcaccggt ctatggcggt gttggcctcg agaagcaggc cgagcgagcc
     4021 cgcaaggcgc acatcgtcgt agcgacgccc ggtcgtttga ccgatctcgt caaccgcaga
     4081 ctgctctcgc tcgacaacat c
//
LOCUS       DATMED010000265         3507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_216686, whole genome shotgun sequence.
ACCESSION   DATMED010000265 DATMED010000000
VERSION     DATMED010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216686"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VNP73_06795"
     CDS             complement(<1..1526)
                     /locus_tag="VNP73_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65665.1"
                     /translation="MRVANKPARWLVGTAVAIVAVMAAGPVTAAEPIDMWEVLVGIDC
                     LNDSFNPTLEDGTKDPNGDPAPGSPEWAERDNERLQCDNQRDHDRRAQPFAHTTSARY
                     GEDWYRQPFLYDDVRFRYDYFPTGTGPGGVPAMEVYRPCSSGDCTDQPEALEEFEAPY
                     PVVIIHHGFIAQMTHHRFNAQVFAEAGYLAITVNGTHPATGAPNVQRNVNAGLVLDWL
                     ASPDSGEIGEQADLDRVATVGHSQGSAAALSYQGDPRVHTIVAWDGGDSIADVNTSQP
                     IMYQRTDGAFSAGQNTARTDYPAGRDRGLETYVKHKERGLDVMHLTFRATNHIDWNGN
                     GVGSLAGNRLAELVINYYSLAWLDRHLQGRLVFDEAGNVVTSDGRTDEEERAYRQAEA
                     QDAYDRLTAMQFDDSVDIHNISMGFYDPMQHANSGDPLYGGNVPYSIDGLWITDRLAQ
                     EFRSFCSVSVPDYVGGSDGSPGSPVVASADSGAEGDMRFTGCRATESLTDTGMTLTAR
                     G"
     gene            complement(1638..3302)
                     /locus_tag="VNP73_06800"
     CDS             complement(1638..3302)
                     /locus_tag="VNP73_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL65666.1"
                     /translation="MDDPRLSQPTYTESVIEDLSITTSDGETLNARVLRPVIPEGTRI
                     PAVMHLSPYLGADIAPETILPELQKHDYVERGYALVAVSVRGSGASGGCLDYQGERDR
                     ADTDSILDTIAAQPWSNGRVGAMGLSWDGTTLNAAALSGNPHLATIVPVGSITDWYKW
                     SFIDGIPGWYVGYIFNIYAPPVVQGAFGRGIPPEQAADRACVNAVDAVVAQEETALTG
                     VRNEWWDERDFVPLADQIDPNLAVLQVQGYLDIGVRPDHLHAWDAALRRRLPNYRLLM
                     GSWGHLWPDTPDVPAFSNPDVEFNRHPLTSWPVILLRWYDHFLKGTDTGITEFPGALM
                     QDQRGEWHGEDGLEPSRAVATRFHPGPQGTLGMEPASGEVSFVDNGQGVDPRGTCLYV
                     GVGWILGCAPVQPETAQFFVTDPYGSETRFSGIGTLDLTLDHSAPRGQVGATLYDVDG
                     DRWKPLTYGLSSLNLRDGNDYEFDPIEPGEAFTHEVELIARDFVLAPGHSLGLAIGSL
                     VDMHPRGLGGNGYAPIPSGGTTTVHLGGTVLELPLLSGETRTSPIP"
BASE COUNT          591 a         1133 c         1120 g          663 t
ORIGIN      
        1 cctcgcgctg taagcgtcat cccggtgtcc gtgagcgact ccgtcgcccg gcatcccgtg
       61 aagcgcatgt cgccttcggc gccgctgtcg gcactcgcaa ccaccggcga gcccggtgat
      121 ccgtcgctac caccgacata atcgggcacc gaaacggaac agaacgaacg gaactcctgc
      181 gcgaggcggt cggtgatcca caggccatcg atcgaatacg gcacgttgcc gccgtagagg
      241 gggtcgcccg agttcgcatg ctgcatcggg tcgtagaagc ccatgctgat gttgtggatg
      301 tcgaccgaat cgtcgaactg catcgcggtg agtctgtcgt acgcatcctg cgcttccgcc
      361 tgacggtagg cgcgctcctc ttcgtcggtc ctcccgtcgg aggtcaccac gttgccggcc
      421 tcgtcgaaga caagtcttcc ctgcagatgg cggtcgagcc acgccaggct gtagtagttg
      481 atgacgagct cggcgagcct gttccccgca aggctcccca caccattgcc gttccagtcg
      541 atgtggttcg tcgcacggaa cgtgaggtgc atgacatcga gaccgcgctc cttgtgtttc
      601 acatacgtct cgagcccacg atcgcgaccc gcggggtagt cggtgcgcgc cgtgttctga
      661 ccggccgaga aggccccgtc ggtgcgctga tacatgatcg gttgcgacgt gttgacgtcg
      721 gcgatgctgt cgccaccgtc ccaggcgacg atcgtgtgga cacgggggtc gccttgatag
      781 ctcagcgcag cagccgaccc ttgtgagtga ccgacggtcg caacgcggtc cagatcggcc
      841 tgctcgccga tctcgccgga gtcgggggat gcgagccaat ccagaaccaa gcctgcattg
      901 acgttcctct gcacgttcgg ggccccggtc gcgggatggg ttccgttcac cgtgatagcg
      961 aggtaacccg cttccgcgaa gacctgggcg ttgaaccgat ggtgcgtcat ctgagcgatg
     1021 aaaccgtgat ggatgatcac taccgggtat ggcgcttcga actcctccag agcctcgggt
     1081 tgatccgtgc aatctccgga cgaacacggc cggtagacct ccatcgcagg gacaccgccc
     1141 gggccggtgc cggtcgggaa atagtcgtag cggaatcgca cgtcgtcgta caggaacggc
     1201 tgtcgatacc agtcctcccc gtaccttgcg ctcgttgtgt gtgcgaaagg ctgcgcgcga
     1261 cggtcgtggt cgcgctggtt atcgcactgg agacgctcgt tgtctcgttc ggcccattcc
     1321 gggctacccg gagccgggtc gccgttggga tccttggtgc cgtcttctag agtcggattg
     1381 aaactgtcgt tgaggcagtc gatcccgacc aggacctccc acatgtcgat cggttcggcg
     1441 gcggtcaccg gccccgcggc catcacggcc acgatcgcta cggcggttcc gaccagccat
     1501 cgagcaggtt tgtttgcaac tctcatgcgc acgcatggct cccttcgagg acgcggctcg
     1561 cctcccgctg ggcgggccgt cgtgcatgat ttcgacgtga ggtgcttggt ttcctgtccc
     1621 gggtgacggc cgatgcccta agggatcggg ctggttctgg tttcgcccga cagcagcgga
     1681 agctccagca cggtccctcc tagatgaacg gtggtcgttc ctccgctcgg gatcggtgcg
     1741 tacccgttgc ctccgaggcc tcggggatgc atatcgacga gagagccgat cgccagaccc
     1801 agactatggc cgggggccag tacgaaatct cgagcgatca actcgacctc gtgcgtgaac
     1861 gcctcgccgg gttcgatcgg atcgaactcg tagtcgtttc cgtccctgag gttgagcgac
     1921 gagagtccgt acgtcagggg cttccaccga tccccgtcga cgtcgtaaag cgtggcaccg
     1981 acctgccccc gaggagccga atgatcgagc gtcaggtcga gtgtgccgat gccgctgaac
     2041 cgggtctcgg atccatatgg gtccgtcacg aagaattgcg ccgtttcagg ctggaccgga
     2101 gcacagccga ggatccaccc caccccaacg tagagacatg tgccgcgcgg atcgacccct
     2161 tgaccgttgt ccacgaacga cacctcaccg cttgcaggct ccatgcccaa cgtcccttgg
     2221 gggccgggat ggaagcgcgt cgcgaccgcg cgcgagggct cgagaccatc ttctccgtgc
     2281 cactcgcccc gttggtcttg catcaaggcg ccgggaaact ccgttatccc cgtgtccgtt
     2341 cccttgagga agtggtcgta ccagcgaagc aggatcaccg gccaactcgt tagtgggtgg
     2401 cggttgaact caacgtcggg attgctgaac gcgggtacgt cgggcgtgtc gggccacaag
     2461 tgaccccagc tccccatcaa caaccggtag ttggggagcc gcctccgaag cgcggcatcc
     2521 cacgcgtgaa ggtgatccgg gcgcacgccg atgtcgagat aaccctgcac ctgcaacacc
     2581 gcaagattgg gatcgatctg gtcggcgagg ggcacgaagt ccctctcgtc ccaccactcg
     2641 ttgcgaacac ccgttaacgc cgtctcctcc tgggcgacga cggcgtccac ggcgttgacg
     2701 caggcgcggt cggcggcctg ctcgggaggt atcccacgtc cgaaagcccc ctggacgacg
     2761 ggaggcgcgt agatgttgaa gatgtatccg acataccaac cggggattcc gtcgatgaag
     2821 ctccacttgt accaatccgt gatgcttccg accggcacga tcgtcgcgag gtgcggattt
     2881 ccgctcagcg cggccgcatt cagtgtcgtt ccatcccacg acagccccat ggctccgacc
     2941 cgcccgttgg accacggctg ggccgcgatc gtgtccagga tcgagtcggt gtcggcgcgg
     3001 tctcgttcac cctggtaatc gaggcatcct cccgatgccc cgctcccgcg cacgctcacg
     3061 gcaaccagcg cgtacccgcg ctcgacgtag tcgtgcttct ggagctccgg aaggatcgtt
     3121 tcgggggcga tgtcggcgcc cagataagga gagaggtgca tgaccgcggg gatccgggtt
     3181 ccttcgggga tcaccggcct gaggacccgt gcgttcagag tctcgccgtc gctcgttgtg
     3241 atggagaggt cctcgatcac cgactccgtg taggtcggct gtgaaagccg cggatcgtcc
     3301 atcgaccatc cctgccacca ctccggcccg gatgctggtg ggcagtgctt ggggttccgg
     3361 ggcttatcgc atggtggcga agcggcggcc ggcaccgtca ttgacgcgag gacgaacgca
     3421 gtgacaacgg cgccgagggt cttcttcaat ggaacccctt agaagtggtc cacgcgtgcg
     3481 cgcggaacgg taaggaccca attcgct
//
LOCUS       DATMED010000266         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3290637, whole genome shotgun sequence.
ACCESSION   DATMED010000266 DATMED010000000
VERSION     DATMED010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3290637"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VNP73_06805"
     CDS             <1..1498
                     /locus_tag="VNP73_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65667.1"
                     /translation="VALEVKLPDRSITSQDELDELLTSEFGADPHVLAQLTIMIHGGA
                     VETFQGEFDLQDHLAAVFGVTPLFEAARNAEAMAARAASALRKMKTTQRSEKREKDDI
                     VAELQSVIEQLEATNAERDQVVATTGAAEETIRIGEEWTRFQAGMDERTEKLSALAQE
                     SSTVVDRPLTAANVLDVLQEAERSLADAVGAAEAEAASARGKTDLINAALSDLEGAEA
                     VCPTCLRPMSEHEAGRAGEEHAKHLRELDTQIQAAEGKANAGRTSLESVRRVLDRVRS
                     LPVPLQPEIEVGSDLAAARTELERRKEELQTIDKKLALLSTTGASLQNALQELEEDEQ
                     QKRELEILYREEGIALATQQALADTGNAITERYIEPLAKEVESRWKTMFGSGGLNLSS
                     EGRITRQVGGRTLEFAALSGGEKVWALLLTRLLIAGASTKAPFIWLDEPLEHLDPKLR
                     KVVAGTLAKASSGAGLRQVIVTTYESELARRLMEDVPSASLIYVTTSA"
     gene            1591..2613
                     /locus_tag="VNP73_06810"
     CDS             1591..2613
                     /locus_tag="VNP73_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWL65668.1"
                     /translation="MTQTTTAQQPVESIELRLERHRVELTAYCYRMLGSTFEAEDAVQ
                     EAFLRAWRSFDSFEGRAAFRSWLYQIATNVCLDMLNGRQRRARPMDLGPAQTADTPLG
                     PRVRETNWIEPVPDSLVLQAESDPAVTAELRESIRLAFVAALQHLAPKQRAVLILKDV
                     LGWRASEIAGLLDTTVAAVTSALQRARATMSNNEVTSTGPHQPLDANHEALLALYVDA
                     FERYDMDALTALLHQDATWSMPPYELWLNTHGDITRWCLGRGIGCRGSLLFRTEANGS
                     PAFAQYKPAPDGGYDAWSLQVVEICDGRIAGITFFLDTEWIFPMFDLPPRLSSEPFSQ
                     RPRLVR"
     gene            2610..3344
                     /locus_tag="VNP73_06815"
     CDS             2610..3344
                     /locus_tag="VNP73_06815"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWL65669.1"
                     /translation="MSTVLGIWAHPDDEVFVSGGLMADAVHNGDRVVCLHMTRGEAGL
                     HFRRPYSPEALGVIREQELEAALACLGVTEQRFLDYPDGDLSLVPGSEVVARIHDVLV
                     EIRPDTVVTFGPDGFTGHPDHKSLSRWVTTAVRLWNKPRAKVLHAIVPSEWKNSVVHR
                     LNEFDFFYPGHPATSGDGDIVTRLDEDTIEAKVDALWEHAGQMQPLFDAYGEEFMRSM
                     ASIEVFRVGPKAAFRSRVENELRIAR"
     gene            3364..>3732
                     /locus_tag="VNP73_06820"
     CDS             3364..>3732
                     /locus_tag="VNP73_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65670.1"
                     /translation="MKASWCALAGAVVLAMAACSQVGSPDTASSPRASRERSSPRAQK
                     TTVPKVVPGGGGKGNGITLPEDLHLRYRVRSSTGDPGWVETDILKSDFRVRFLDEDNR
                     PQLELIYLNGRYFFCEHSAAY"
BASE COUNT          704 a         1199 c         1208 g          621 t
ORIGIN      
        1 ggttgccctc gaggtgaaac tgccggatcg gtccatcacc tcgcaggacg aactcgacga
       61 gctgctcacc tccgagttcg gtgccgatcc tcacgtcctc gcccagctga cgatcatgat
      121 ccacggcggt gccgtggaga cgttccaggg tgagttcgac ctccaggacc accttgctgc
      181 cgtcttcgga gtgacgccgc tgttcgaagc ggccaggaac gccgaggcga tggcggctcg
      241 agcggcaagc gcgctgcgga agatgaaaac gacccagcga tcggagaagc gcgagaaaga
      301 cgacatcgtg gccgagctcc agtccgtcat agagcaactc gaggcaacca acgcggagcg
      361 cgaccaggta gtggccacca ccggtgccgc cgaagaaacg atccgcatcg gcgaggagtg
      421 gacgcgcttc caagccggta tggatgagcg aacggagaag ttgtccgctc tggctcagga
      481 gtcgtcgacg gtcgtggatc gaccgctcac ggccgcgaac gtcctcgacg ttctgcaaga
      541 ggccgagagg agcctggccg atgccgtcgg tgccgccgaa gccgaagctg cgtctgcacg
      601 gggaaagacc gatctgatca acgccgcact ctcagacctg gagggtgccg aggccgtctg
      661 cccgacctgt ctgagaccga tgtccgagca cgaggccgga cgcgccggcg aggagcacgc
      721 gaagcacttg cgcgagttgg acacgcagat ccaggccgcc gagggcaagg ccaacgccgg
      781 ccggacatct ttggagtcgg tacgcagggt gctcgaccgg gtgcgctcgt tgcccgttcc
      841 cctgcagccc gagatcgagg tcggatcgga tctcgccgct gctcgaaccg aactggagcg
      901 tcggaaggaa gagctgcaga ccatcgacaa gaagctcgcg cttctatcga ccactggggc
      961 ttcgctacag aacgcgctgc aggaactcga agaggacgag cagcagaagc gtgagttgga
     1021 gatcctctac cgcgaggaag ggatcgcgct cgcgactcaa caagcgctcg cggatacggg
     1081 gaacgcgatc accgagaggt acatcgagcc cctcgccaaa gaggtcgaga gccgctggaa
     1141 gaccatgttc ggatcgggcg gcctgaacct cagttccgag gggcgcatca cacgtcaggt
     1201 cggaggtcga accctcgagt tcgcggcttt gagcggcggc gagaaggtgt gggcgttgct
     1261 gctcacgcgc ctcctgatcg caggggcctc caccaaggcc cccttcatct ggttggacga
     1321 gccgctcgag catctcgacc cgaagctccg gaaggtcgta gccggaaccc tggccaaggc
     1381 gtcgtcgggg gccgggctcc gacaggtcat cgtcactact tacgagagcg agctcgcccg
     1441 caggctgatg gaggacgttc cctccgcgtc gctgatctac gtaacgacct cggcctagaa
     1501 ccagtctcgt aacgctttcg cgacctcact ttcgattctc cgcgtctttc gattacccct
     1561 cgagaccgcg tgagacctgg aggtgaggaa gtgacgcaga caaccacggc gcagcaaccg
     1621 gttgagtcga tcgagctccg gctggagcgg catcgcgtgg agctgaccgc gtactgctac
     1681 cggatgctgg gctcgacctt cgaggcggaa gacgccgtgc aggaggcctt cctacgagcg
     1741 tggcgatcgt tcgattcgtt cgagggcagg gccgcgttcc gctcgtggct ctatcagatc
     1801 gccaccaacg tctgcctcga catgctgaat gggaggcagc gacgcgctcg tccgatggac
     1861 ctcggaccgg cacagaccgc cgataccccg ctcggccccc gcgttcgaga gaccaactgg
     1921 atcgagccgg tgccggactc cctcgtcctg caagcggagt ccgacccggc cgtcaccgcc
     1981 gagctgcgcg agtccatccg tctcgcattc gttgccgcac ttcaacacct agcgccgaag
     2041 cagcgcgcgg tcctgatcct caaggacgtg ctggggtggc gggcgagcga gatcgccggt
     2101 ttactcgaca cgaccgtcgc tgcagtgacg agtgccctgc aacgggctcg agccacgatg
     2161 tcgaacaacg aagtcacatc gacgggcccg caccagcctc tcgacgcgaa ccacgaagca
     2221 ttgctcgccc tctacgtcga cgctttcgag cgctacgaca tggacgcgct caccgcgctg
     2281 ctccaccagg acgccacctg gtcaatgccg ccgtacgaac tgtggttgaa cacgcatggg
     2341 gatatcactc ggtggtgcct cgggcggggg atcgggtgcc gcggctcgtt gctgttccgc
     2401 accgaggcca acggctcgcc ggccttcgcg cagtacaaac ctgcgccgga cggcggctac
     2461 gacgcatggt cgctccaagt ggtcgagatc tgcgacggca ggatcgccgg gatcaccttc
     2521 ttcctcgaca ccgagtggat cttccccatg ttcgaccttc cgcccagatt gtcgagcgag
     2581 cccttctcgc agaggccgag gctcgtccga tgagtaccgt tcttgggatc tgggcgcatc
     2641 cggacgacga ggtgttcgtg tccggaggac tgatggcaga cgccgtgcac aacggcgatc
     2701 gggtcgtgtg cctccacatg accagaggag aagcaggcct ccacttccgc cggccttact
     2761 cccccgaggc tctcggcgtc atccgagagc aagagctcga ggcggccctc gcgtgccttg
     2821 gtgtgaccga gcagcgattc ctcgactacc ccgacggcga tctgtccctc gtgccaggta
     2881 gtgaggtcgt tgcccggatc cacgacgtat tggtcgagat ccgtccggac acggtcgtga
     2941 ccttcgggcc cgacggcttc accggtcacc ccgaccacaa gtcgttgtcc cggtgggtga
     3001 ccaccgcggt gaggctgtgg aacaagcctc gggcgaaagt gctgcatgcg atcgttccga
     3061 gcgagtggaa gaactcggtc gtccatcggc tgaacgagtt cgacttcttc taccccggac
     3121 atcctgcgac gagcggagac ggcgacatcg tgacccggct ggacgaggac acgatcgagg
     3181 cgaaggtcga tgccttgtgg gagcacgcag gccagatgca gcccctcttc gacgcctacg
     3241 gcgaagagtt catgcgctcg atggcatcga tcgaggtctt tcgggtcgga cccaaggccg
     3301 cgttcagatc gcgggtggaa aacgagttaa ggatcgcccg gtaagcgaaa tacgatgacc
     3361 cacatgaagg cttcatggtg tgccctcgcg ggtgccgtcg tgctcgcgat ggcagcgtgc
     3421 tcgcaggtcg gaagtcccga taccgcgtcc agcccgcggg cgagccgtga gcgatcgtcc
     3481 cctcgagcac agaagaccac ggtgccgaag gtggtcccgg gcggcggagg taaggggaac
     3541 gggatcaccc ttccggaaga cctccatctt cgctaccgcg ttcgatcatc gaccggtgac
     3601 cccggctggg tcgaaaccga catcttgaag tccgacttca gggtccgatt cctggacgaa
     3661 gacaaccggc cgcagcttga actcatctat ctcaacggca ggtacttctt ctgcgagcac
     3721 tcggcggcct at
//
LOCUS       DATMED010000267         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3290919, whole genome shotgun sequence.
ACCESSION   DATMED010000267 DATMED010000000
VERSION     DATMED010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3290919"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..479
                     /locus_tag="VNP73_06825"
     CDS             75..479
                     /locus_tag="VNP73_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWL65671.1"
                     /translation="MSTRFEPPISDISASFWDATKNREFVLQHCRTCDKAVHYPRAIC
                     PHCHSTELEFRPASGRGEVYAFSVMHRAGNPTMEDRVPYVVALIDLDEGARMMSNVVG
                     CDHAEVKVGMQVQLTWEELSDGRNLPLFEPAE"
     gene            491..1693
                     /locus_tag="VNP73_06830"
     CDS             491..1693
                     /locus_tag="VNP73_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL65672.1"
                     /translation="MRIDLGPEVEKFRGEVRDWIKTNRFQELDQIDEEEYNLFASPSE
                     AVQEWVSRLRKDRWLCVAWPEEYGGRGLGPLELSVVNQEFAAAKVPRITMGMGEWLVG
                     PSIIAHATEEQKAFFLPRIVSGEDRYCQGFSEPDAGSDLAGLKTKGVVEGDEIIVTGQ
                     KVWTSGASRCNMVFTLARTDPEAPKHNGISYVLVPMDDNNVTYRPIKQITGESHFFEV
                     FYDGARAPMFNVIGGLNNGWKVAMTTLGNERGATAATQHLRFEGELWDLVDLAKKLGK
                     SEDPLVRQHLAWAYTHVELMRMQGLRILAGIAAGKGLGPESSINKLFWSEYHKRLGEV
                     AMEILGPAGFATAGANGVDAQRWWHTFLSARAETIYAGTSEIQRKIISERVLGLPRES
                     QVKKAQSA"
     gene            1693..>2887
                     /locus_tag="VNP73_06835"
     CDS             1693..>2887
                     /locus_tag="VNP73_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL65673.1"
                     /translation="MAPEAGPLAPPSPDKLLMERIEAATRGMGLSVTARYFGDRVALI
                     SEHGDRTFGQLNARANQVARALRARGLNEDDAVGLICSNRPEFPETIYASQRSGLRTV
                     PINWHLTAEETSYIVRDSEAKAIVADARFSETVAEAARLAPDATVRLSIGGDIDGFES
                     LEDSVKEEDESDLTDAVLGRHMLYTSGTTGRPKGVFRPGGMQGAAQMLQIYGPILERY
                     AFGENDVNLVTGPLYHSAPLAFSLIVPIMAGVRSVLMDGWDPETTLRLIEKHKVTHAH
                     MVPIMFHRLLSLPADVRARYDTSSLRFILHGAAPCPVEVKKSLIEWLGPVVHEYYAAT
                     EGLGTFVFAEEWLERPGTVGKIEDEKVEVRGSDGKRLPAGEVGFVFIHSDPDLKFEYY
                     KDPAKT"
BASE COUNT          544 a          904 c          927 g          512 t
ORIGIN      
        1 atcgcggtgg cacacggcag cggtggcgtc ctctcttcta tgtctacgat cgtgcttggt
       61 agcgaggaaa cgttatgagc actcgtttcg agcccccgat ctccgacatc tccgcttcct
      121 tctgggacgc gaccaaaaac cgagagtttg tccttcagca ctgcaggacg tgcgacaaag
      181 ccgtgcatta cccgcgcgcg atctgccctc actgtcattc gaccgagctc gagttccgac
      241 cggcatcggg tcggggtgag gtctacgcgt tcagcgtcat gcatcgcgcc ggtaacccaa
      301 cgatggaaga ccgggtgcct tacgtcgtcg cgctgatcga tctcgatgag ggcgctcgga
      361 tgatgtcgaa cgtagtcgga tgcgatcacg ccgaggtcaa ggttgggatg caggttcaac
      421 tgacatggga agagctttcg gacggccgca acctgccgct gttcgagcct gccgagtaag
      481 ggggaaatgc atgaggatcg atctggggcc cgaggtcgag aagttccgtg gtgaggtgcg
      541 cgactggatc aagacgaatc gtttccagga gctcgaccag atcgacgaag aggaatacaa
      601 cctgttcgcg agcccgtcgg aggccgtgca ggaatgggtc tctcgcctgc ggaaagaccg
      661 ctggctgtgc gtggcgtggc ccgaggagta tgggggccgc ggtctcggcc ccctcgaact
      721 ctccgtggtc aatcaagagt tcgccgccgc taaggtgccc cggatcacga tggggatggg
      781 cgagtggctc gtcggcccct cgatcatcgc ccacgccacc gaagagcaaa aggcgttctt
      841 cctcccccgc atcgtctccg gtgaggaccg ctattgccag ggcttctcgg aaccggatgc
      901 gggttccgat ctcgccggtc tgaagacgaa gggtgtcgtg gaaggcgacg agatcatcgt
      961 caccggtcag aaggtgtgga cctccggagc gtcgcgctgc aacatggtgt tcacactggc
     1021 ccgtaccgac cccgaggccc ccaagcacaa cggcatctct tacgtcctcg tcccgatgga
     1081 tgacaacaac gtcacctatc gaccgatcaa gcagatcacg ggagagtcac acttcttcga
     1141 ggtcttctac gacggcgccc gcgccccgat gttcaacgtg atcgggggct tgaacaacgg
     1201 atggaaggtc gcgatgacga cgctcggcaa cgagcgcggt gctacggcgg caacacaaca
     1261 cctccggttc gaaggagagt tgtgggacct cgtcgatctt gcgaagaagc tgggaaagag
     1321 cgaagaccct ctcgttcgtc aacatctggc gtgggcctac acgcacgtcg aattgatgcg
     1381 gatgcaaggg ttgcgcatcc ttgccggcat cgccgcaggc aagggactcg gccccgagtc
     1441 ctcgatcaac aaactgttct ggtccgagta tcacaagcgc ctcggcgagg tagcgatgga
     1501 gatcctcggt ccggccgggt tcgcgacggc aggtgcgaac ggtgtcgacg cacagcggtg
     1561 gtggcacacg ttcctctcgg cacgggccga gacgatctac gcgggcacga gtgagatcca
     1621 gcgcaagatc atctcggagc gcgtgctcgg gctccctcgc gaatctcagg tcaagaaggc
     1681 acagagcgcg taatggcgcc cgaagcagga ccgctcgcac cgccgtcgcc cgacaagctc
     1741 ctgatggagc gcatcgaagc ggcgacgcgc ggtatgggcc tgtcggtcac tgcccgctat
     1801 ttcggcgatc gcgttgccct gatctccgaa cacggcgacc gcaccttcgg ccagctgaac
     1861 gcgcgcgcca accaggtcgc gcgtgctctg cgggcccggg gactgaacga ggacgatgcc
     1921 gtcgggctca tctgttccaa ccgtcccgaa ttcccggaga cgatctacgc ctcgcagcgg
     1981 tcgggcttgc gaacggtccc gatcaactgg cacctgacgg cggaggagac gtcgtacatc
     2041 gtgcgcgact ccgaagccaa agcgatcgtt gcagacgcgc ggttctcgga gaccgttgcc
     2101 gaggccgcgc ggctggcgcc cgacgccacg gtgcgactgt cgatcggcgg cgacatcgat
     2161 gggttcgagt cgctcgaaga ctcggtcaag gaagaagacg agagcgacct gaccgatgcg
     2221 gtgctcggcc ggcacatgct ctacacctcg ggcacgaccg ggcgacctaa gggagttttc
     2281 cggcccggcg gcatgcaggg ggccgcgcag atgctccaga tctacggccc gatcctcgag
     2341 cgatacgcgt tcggcgagaa cgacgtgaac ctggtcaccg ggccgctgta ccactcggcc
     2401 cccctagcgt tctcgctgat cgtcccgatc atggccgggg tccgctcggt cctgatggac
     2461 ggctgggatc ccgagaccac cttgcgcttg atcgagaagc acaaggtcac ccacgcgcac
     2521 atggtcccga tcatgttcca tcgtctgctc tcactccccg ccgatgtgcg tgctcgttac
     2581 gacacatcgt cgcttcgttt catcctgcat ggcgcggcgc cttgcccggt cgaagtgaag
     2641 aagtcactga tcgagtggct gggaccggtc gtgcacgagt actacgccgc caccgagggc
     2701 ctcggtacct tcgtgttcgc ggaggaatgg ctcgagcggc ccggcacggt cggcaagatc
     2761 gaagacgaga aagtcgaggt ccgcggctcc gacggcaagc gtcttccggc cggcgaggtc
     2821 ggctttgtct tcatccactc cgatcccgat ctcaagttcg agtactacaa ggaccccgcc
     2881 aagaccg
//
LOCUS       DATMED010000268         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2527919, whole genome shotgun sequence.
ACCESSION   DATMED010000268 DATMED010000000
VERSION     DATMED010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527919"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..2737)
                     /locus_tag="VNP73_06840"
     CDS             complement(149..2737)
                     /locus_tag="VNP73_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWL65674.1"
                     /translation="MNGFVTLVRRMNLRHFREKRMRTLLTLSGVASGVMLVFSIGLIN
                     ATLLESVRSSVRALAGAAEIEVATADRNGIPVAAARTIQDVEGVDRAVPVLRATALVH
                     GDSTTSRPMIVGITPDFGALFPKSAGEFGRISITGGFGASGGGLLLADRVAGELDVKV
                     GDRVEVETPSGIEQVAVTGRISGDAVDFLNGGQMGLMLLEGAQRTFDRPDRIDSVYVI
                     TDRDASVRDVEQRIASALEVPTIIGPPGERGSGFEEQVGSVSTLTSLAGTVALLVSIF
                     VVFNTMSMSLAERRREISMALALGASKRQMFAAFLSEAATLGAVAAASGIGAGYLLAR
                     VLIEPVVEGFPVLPISGTNPVVVPVGQVVIAAVGGLVVAIAGAYVPARRVLKVAPVES
                     LRPQAAYEWDGSRRHPSLKTTLVVALGPVALSVMAAVAYITAFNDQPWLVNLSLTLGL
                     IGISVLLLRAVPFGVQVLRIPFVRLFGTVGRLATDALSKNPGRTTFTVAALILTLGMV
                     VGVASSLGSYEKELDRSAHQWMGAEIYVGSSSYQALGADQPLAGNTEQVFADVPGVET
                     VYPVRYGFLDVDGSQMMIYAIPAARALEEGFGVISTVEGIPHDELVEGISAGRVFIAR
                     YTAEEQGWDTGDRIMLPTPKGERQLVVGGVFEDLNSFQSMFIEHEVYSRIWSDDKIDR
                     LAVILEDGASVGEVKAALTAVVKKKNITARVLTQDEAVGELVDTVEGLFQMARGIQFA
                     ALVVALLTIANTMFTAVLERRWEMGLQRAIGMGRAQIGRSLFLETLGIGLIGGAGALI
                     MGTATGLLMTKLMEAQYAWRIPYQAPAGLIALAIAAGVVIAVVAGALPSRMAVRAPII
                     ESLRYE"
     gene            complement(2734..3459)
                     /locus_tag="VNP73_06845"
     CDS             complement(2734..3459)
                     /locus_tag="VNP73_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65675.1"
                     /translation="MTVDLSGEPTVKTDQKVETITARGLVKTYRQGDEEIHALRGVDL
                     SVRAGEFLAITGASGSGKSTLMHILGGLDRPDQGEVLLEGKRISKLSDEELSVVRRRR
                     LGFVLQFFNLLPTMTAAENAAFPLLLDGNSDALERACESLAQVGLESRVDHRPAHLSG
                     GEQQRVALARALVTQPAVVLADEPTGNLDSLTGEDILKLLRRTADAGQTIVMVTHDAR
                     SASYADRVVRLVDGLLSEQGTTA"
     gene            complement(3456..3980)
                     /locus_tag="VNP73_06850"
     CDS             complement(3456..3980)
                     /locus_tag="VNP73_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWL65676.1"
                     /translation="MSVPYVLLGLLEESPRHGYELKRDYDELVGQSKPVKFGQIYATL
                     GRLERDGLIDLAAEERGQGPDRKRYAITENGVTDLDQWLSEPETAEPHMHNVLFTKVV
                     LAMFSNREPELFLNAQRAEHQRRMRDLTRIKEEGSLAAALASDYALFHLEADLRWIEL
                     ASARLERLRKEVKK"
BASE COUNT          780 a         1355 c         1254 g          718 t
ORIGIN      
        1 cggcatcccg cttattgccc ttgtcctcgc cttcgtccgc ggcggagggg gcggtcgtcc
       61 cggaggaagt ggactcgggg gcgttgctac acgcgccgag gaacagcatc agagcggccg
      121 aaacggcgat gagctttttc ataggacctc attcataccg gagagattcg atgatcggtg
      181 cgcgcacggc catccgggac ggcagcgctc ccgcaacgac ggctataaca acgccggctg
      241 cgatcgcaag agcgatgaga ccagccggag cctgatacgg gatccgccag gcgtactgcg
      301 cctccatcag tttcgtcatc aataggcccg tggccgttcc catgatcaaa gcacccgcgc
      361 ctccgataag accgataccc agggtttcga ggaacaggct ccgaccgatc tgagcgcggc
      421 ccataccgat cgcacgctgc agacccatct cccaccggcg ttcgagcact gccgtgaaca
      481 tcgtgttggc gatcgtgagc aaagcaacga cgagcgccgc gaactggatc ccgcgcgcca
      541 tctggaatag accttcgacg gtgtcgacaa gttccccgac cgcttcgtcc tgggtgagga
      601 cccgtgcggt gatgttcttc ttcttgacga cggcagtcag cgcagctttg acctcaccga
      661 ccgacgctcc gtcttcgagg atcacggcca gccggtcgat cttgtcgtcg ctccaaatcc
      721 gggagtacac ctcgtgctcg atgaacatgg actggaacga gttcaggtcc tcgaacacgc
      781 ccccgacgac caactgccgt tctcccttgg gcgtcggcaa catgatccgg tctcccgtgt
      841 cccaaccctg ttcctccgct gtgtagcgag cgatgaacac gcgtcccgcc gagatcccct
      901 cgaccaactc gtcgtgaggt atgccctcga cggtcgagat gactccgaag ccttcttcca
      961 atgcccgcgc ggctgggatc gcgtagatca tcatctggct gccgtccaca tccaggaacc
     1021 cgtagcgaac cgggtacacg gtctcgacgc cggggacgtc tgcgaaaacc tgttcggtgt
     1081 ttcctgccag gggctgatcg gcgccaagag cctgatagga gctggacccg acgtagatct
     1141 cggcccccat ccattgatga gccgatcggt cgagctcctt ctcgtaagag cccagcgaac
     1201 ttgcaacccc cacgaccatg cccagcgtca ggatcaaagc agcgacggtg aaggtcgtcc
     1261 gccccggatt ctttgagagc gcatcggtgg cgagccgtcc cacggttccg aacagcctga
     1321 cgaaggggat gcgcagaacc tggacgccga acggaacggc cctaagcagc aacacgctta
     1381 tgccgatcag ccccagggtc aaggacaggt tcacgagcca cggctggtcg ttgaacgcgg
     1441 tgatgtaggc gaccgccgcc ataaccgaca gcgcgaccgg acccagagcg accaccaacg
     1501 ttgtcttgag actcggatgc cgccggctcc cgtcccattc gtaggcagcc tgcggacgaa
     1561 gagactcgac gggagcgacc ttgagcacgc gacgcgccgg aacgtacgcg ccggctatcg
     1621 cgacgacgag acctccgacg gcagctatga ctacttgccc caccggtacg accaccggat
     1681 tcgtcccgct tatgggcaga acagggaaac cctcgacgac cggctctatg agaacgcgcg
     1741 ccagtagata gccggcccct atgccgcttg cggcggctac cgcacccagg gtcgcggcct
     1801 cactgaggaa agcggcgaac atctgccgtt tcgacgcgcc cagggcgagt gccatcgaga
     1861 tctcacgcct gcgttcggcc agcgacatgg acatcgtgtt gaagacgacg aagatcgaga
     1921 cgagtagagc gacggttccg gcaagtgatg tcagcgtcga gaccgaaccc acctgctctt
     1981 cgaacccgct accccgttct cccggggggc cgataatggt aggaacctcc aacgcggatg
     2041 cgatccgctg ctcgacatcg cgcacgctcg cgtctcggtc ggtgatgacg tagacggaat
     2101 cgatcctgtc gggacgatcg aaggttcgtt gggctccctc gagaagcatc aagcccatct
     2161 ggccgccgtt caggaaatcc accgcatctc ctgatatccg gccggtcacc gctacctgct
     2221 cgatgccgga cggagtttcg acctcgacgc ggtctccaac cttgacgtcg agttcgccgg
     2281 caacccggtc ggccagcaac aagccgccgc ccgaggcgcc gaatccgccc gtgatcgaga
     2341 tacggccgaa ctcgcccgcg gacttgggga acagagctcc gaagtcgggg gtgatgccga
     2401 cgatcatcgg acggctggtc gtcgaatctc cgtgtaccag ggcggtcgcg cgaaggacgg
     2461 gaacggcccg gtcgacaccc tcgacatcct ggatcgtgcg ggcggccgct accgggatgc
     2521 cgttgcgatc cgccgtggcg acttcgatct cagcggcgcc ggccaaagcg cgcacgctgg
     2581 agcgaaccga ctcgagcagc gttgcattga ttagccctat cgaaaacacg agcatgacac
     2641 cggatgcgac gccggacagg gtcaggagcg tccgcatcct tttctcgcgg aagtgacgca
     2701 ggttcatacg ccgcaccagt gtgacgaaac cgttcacgcc gtcgtgccct gctcgctcag
     2761 cagaccgtcg acgagccgca cgactcggtc ggcgtaagag gccgaccgtg cgtcgtgggt
     2821 gaccatgacg atcgtctgcc ctgcgtccgc cgtgcggcgg agaagcttga ggatgtcttc
     2881 gccggtgagc gagtcgaggt tcccggtcgg ttcgtcggcg agcacaaccg ccggttgggt
     2941 gactaacgcc ctagccaacg ccactcgctg ttgttcgccg cccgaaaggt gtgccggcct
     3001 gtggtcgaca cggctttcca gtcccacttg ggccagcgac tcacacgctc gctcgagcgc
     3061 gtcgctgttc ccgtctagca acagagggaa cgccgcattc tcggcggccg tcatggtcgg
     3121 caacaggttg aagaactgca acacgaaacc gagccggcgc ctgcggacca cagacagttc
     3181 ttcgtcggat agcttggaga tgcgcttccc ctcaagcagg acctcacctt gatcgggccg
     3241 atccagccca ccgaggatgt gcatgagcgt cgacttcccg gaaccggatg caccggtgat
     3301 cgcgaggaac tcgccggcac ggacgcttag atccacaccc cgcagagcgt ggatctcttc
     3361 gtctccctga cggtaggtct tcaccaagcc gcgagcggtg atggtctcga ccttctgatc
     3421 ggtcttcacg gtcggctcgc cgctgaggtc caccgtcact tcttcacctc tttccgtagg
     3481 cgctcgagtc gagccgaagc aagttcgatc cagcgcagat ccgcttcgag gtggaacagc
     3541 gcgtagtccg acgcgagcgc ggccgcgagg ctcccctcct ccttgatccg cgtcaggtcg
     3601 cgcatgcggc gttgatgttc ggcgcgctgc gcgttgagaa atagttcggg ctcgcggttc
     3661 gagaacatag cgagcacgac tttcgtgaac aagacgttgt gcatgtgcgg ctcggccgtc
     3721 tcgggctcgg acaaccattg gtcgagatcg gtgaccccgt tctccgtgat cgcatagcgc
     3781 ttgcggtccg gtccctggcc gcgctcttcg gcggcgaggt cgatgaggcc gtcccgttcg
     3841 agccggccga gggtggcgta gatctggccg aacttcaccg gcttggactg gccgaccagt
     3901 tcgtcgtaat cccgcttcag ctcgtagccg tgccggggcg actcttccag aagtcccaat
     3961 aggacataag ggacactcat gggcacaata tacactcggt gtataaaccc ggtgaatacc
     4021 gcctaaaccg tagcggccac tgcgtgggcg cgtgcgtaga cgtcttcgac ccccggcacg
     4081 agtcgcgacc aatcgaagga cgacgcc
//
LOCUS       DATMED010000269         4109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_219501, whole genome shotgun sequence.
ACCESSION   DATMED010000269 DATMED010000000
VERSION     DATMED010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219501"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..314
                     /locus_tag="VNP73_06855"
     tRNA            240..314
                     /locus_tag="VNP73_06855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:275..277,aa:Ile,seq:gat)
     gene            complement(320..601)
                     /locus_tag="VNP73_06860"
     CDS             complement(320..601)
                     /locus_tag="VNP73_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65677.1"
                     /translation="MSVPLSLWWAYALWTGRSWAFSWAFMLTALCAVLLVGVVLVQIL
                     LLEMSVPAGVWWSLFASWLWLFLLIQPETKRFAGLIQEKQPGEELTAGG"
     gene            738..1337
                     /locus_tag="VNP73_06865"
     CDS             738..1337
                     /locus_tag="VNP73_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HWL65678.1"
                     /translation="MTELELLDWKRRIFALYSRVRETTDHETAWKEWVATRDELFRGH
                     SQSPLPADERVGFSGLGYFPFDPDVRVEAKLTQASAQSFELTTSGSETMAFTRFATAA
                     FRLYGSECTLDVYWLDAYGGGIFVPYKDATSGHETYGAGRYLFDTVKGADLGSTQDGL
                     IFDFNFAYNPSCAYDPRWVCPLAPPPNHLELEVRAGELS"
     gene            complement(1383..2333)
                     /locus_tag="VNP73_06870"
     CDS             complement(1383..2333)
                     /locus_tag="VNP73_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL65679.1"
                     /translation="MRRVRNAVLGAVLLSFVLFGAACAGPGGDAGQTEGDTDGGAAKG
                     EVTVGSDSFAEAQIVGEMYALVLENAGYTVERQLDLESREVRLPAMENGEIDVAPEYL
                     ASLYSVLDAKAVEKAEPGELGDPEEVRSRLEPLLAEKGLAVLDHSDVIDTNALVVTQE
                     TAEEHALTTTSDLADVAGDLTLGAPPECPERPFCIPGFEEVYGIEFGDFKSLEYGATI
                     TALSKGAIDVGLLYSTDGTIAENNFVVLEDDKGLQAADNITPLVTEETAGMQEVVDLI
                     NSVTAALETDAITELNKRANVDVEDPADLAQEYLEEKGLL"
     gene            complement(2397..3095)
                     /locus_tag="VNP73_06875"
     CDS             complement(2397..3095)
                     /locus_tag="VNP73_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65680.1"
                     /translation="MSELIQWFSTTETIPEQLVRHLYLSYVPAIAAFLLALPVGLYIG
                     HARRFEFIAITLANLGRAVPSFAILSLALIVFLEISPEHGLGFWPTFAALFFLSVPPI
                     LVNTHIGVKNVDPDTVEAARGLGYPGGRILREIELPLAAPLIVTGMKTAAVQSVATAT
                     LGAFVGAEGLGEFIRLGIRTQSSDVLLGGAILVALLAIMTEALMSILERWVRPRIRRD
                     VRTRPDAYAPASVL"
     gene            complement(3092..3760)
                     /locus_tag="VNP73_06880"
     CDS             complement(3092..3760)
                     /locus_tag="VNP73_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65681.1"
                     /translation="MTPAFFRWDWILDHTEEVYLALGEHFYLTLLSVGVGFLISFPVA
                     VFSWRHRRVYGPITLAAGVMYTIPSLALFALLIPFSGAFSTTTAVIPLIIYTLLILIR
                     NIVAGLDSVDEDVRDAARGMGYSRRQLLWKVELPLALPAVVAGVRIATVTTIGLVTVT
                     AVLGRGGFGYFILLGLTRTFPTAIILGAVASMLLAMGADFVLLRVERWLAPWARSRSV
                     RTTG"
     gene            complement(3757..>4109)
                     /locus_tag="VNP73_06885"
     CDS             complement(3757..>4109)
                     /locus_tag="VNP73_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWL65682.1"
                     /translation="LLKVSDIHVEDGPVVSPSDTVDEARRQMEKFDHDWVSVVEDGHL
                     FGWVNAEMMEGVRTPGEAGPKPFSAYVTRDSTLRQALDSIVTSRTQVAVVASEGQRYL
                     GILTLERISKEIVS"
BASE COUNT          882 a         1308 c         1169 g          750 t
ORIGIN      
        1 tactggtaca acggtcgtat ctacaccaac gactatttgt cgcagttggg cgtcggctca
       61 tacttcgcca agggatacgg gaagaagcag gcctatttct tcaagaagac gccactgaac
      121 ccgaacggcg agttcaaccc gcaggttcag atcccgagtt gggaatagca gctaccagga
      181 actaattcat acagggaggc cgcttcggcg gcctccctta gtatctcggt tcattccacg
      241 ggcccatagc tcagctcggt tagagcgcgc ccctgataag ggcgaggtcg atggttcaaa
      301 tccatctggg cccactcaac tacccgccgg cggtgagctc ctctccgggc tgtttctcct
      361 ggatcagacc ggcgaatcgt ttggtctcag gttgaatcag aaggaagagc catagccaag
      421 aagcaaacag gctccaccag acgcccgcgg gaacactcat ctccaacaac aggatctgga
      481 cgaggacgac tcccaccaac agaaccgcgc acagcgcggt caacatgaac gcccacgaga
      541 aggcccagct cctacccgtc cacagcgcgt aggcccacca cagactgagt ggaacgctca
      601 ctaaatccca gattgtgcga tcggtatcta ggaaaaggct cgagacaagc cctacgagac
      661 tcgctaccac cagcacataa accgtccacg gtcgacctac ccgtgatcct tcgctagcca
      721 ttaatttgag cctacacatg actgagctgg aactgctcga ctggaagcgg cggatcttcg
      781 ccttgtactc ccgcgtccgg gaaacgaccg atcacgagac cgcttggaag gagtgggtcg
      841 caacgcgcga cgagttgttc cgcgggcact cacaatcccc cttgccggca gatgagcgag
      901 tcggtttctc agggctgggc tacttcccgt tcgacccgga cgtccgggtc gaagccaaac
      961 tgacccaggc atcggcccaa tccttcgagc tgacgactag tgggtccgaa acgatggctt
     1021 tcactcgctt cgcgaccgca gcattccggc tctacggcag cgaatgcacg ctcgatgtct
     1081 actggctgga cgcttacggg ggtgggatct tcgtcccata caaggacgct acgagcggcc
     1141 acgagacgta cggcgctgga cgctacttgt tcgacaccgt caagggagcc gacctcggct
     1201 cgacccaaga tggtctgatc ttcgacttca actttgccta caacccgtcg tgcgcgtacg
     1261 acccgcgctg ggtgtgcccg ctcgcacctc cacccaacca tctcgagcta gaggtgcgag
     1321 ccggcgagct cagctaaggc gccgccgcga agcggcaggt ggcgcagtgc tgatcagaag
     1381 gcctaaagga gccccttctc ctcgaggtat tcctgagcca gatcggctgg gtcttcaacg
     1441 tcgacgttgg cgcgcttgtt gagttcggtg atcgcatccg tctctaacgc ggcggtaacg
     1501 gagttgatca gatctacaac ctcttgcatc cccgctgttt cctcagtgac gagcggagtg
     1561 atgttgtctg ccgcctgcaa gcccttgtca tcttccagaa cgacgaagtt gttctccgcg
     1621 atcgtcccat cggtcgagta caacagcccg acgtcgatcg cacctttcga caatgcagtg
     1681 atcgtcgctc cgtactccag cgacttgaag tcaccgaact cgatcccgta gacctcttcg
     1741 aaacccggga tgcagaacgg acgctcggga cactcgggtg gagcacccag cgtcaagtca
     1801 cccgcaacat cggctaaatc ggacgtcgtt gtcaatgcgt gctcttcggc cgtctcttga
     1861 gtcacgacga gtgcgttcgt gtcgatgaca tcggagtggt cgagtacggc aaggcccttc
     1921 tctgcaagaa gcggttccaa ccgggagcgg acctcttcgg gatcgcccag ctcgcccggc
     1981 tccgccttct cgaccgcctt ggcatcgagc accgagtaaa ggctcgccag atactccggg
     2041 gccacatcga tctcgccgtt ctccatcgcc ggcagcctca cctcacgcga ctcgaggtcc
     2101 agctgcctct ctactgtgta acccgcgttc tcaagaacaa gggcatacat ctctcccacg
     2161 atctgtgcct cggcgaaact gtcggatccg accgtgacct cgcccttggc ggcaccccca
     2221 tcggtgtcgc cttcggtctg gccggcgtcc ccacccggtc ctgcacatgc cgctccgaac
     2281 agaacgaacg aaagcaggac cgccccgagg acggcgttgc ggactctccg catgggtcaa
     2341 cactcctttc gggagtagtc ggggtgtccc gccccgctgc ttaagaccgc gccaccttag
     2401 aggacgctcg ccggcgcgta ggcatcgggt ctcgttcgca cgtcgcgcct gatgcgaggg
     2461 cgaacccaac gttccaaaat gctcattagc gcctcggtca tgatcgcgag caaggcaacc
     2521 aatatggcgc ccccgagcaa cacgtcgctg ctctgggttc ggatcccgag tctgatgaac
     2581 tctccgagac cttcggctcc tacgaacgct cctaacgtcg cggttgcaac cgactgaacc
     2641 gcggccgtct tcatccccgt gacgatcagc ggagcggcca gaggaagttc aatctctcga
     2701 aggatccggc ccccgggata tccgaggcct ctcgccgcct caaccgtgtc gggatcgacg
     2761 ttcttaacgc cgatgtgtgt gttcaccagt atcggaggga ccgacaggaa gaacagcgcg
     2821 gcgaaggtgg gccaaaaacc aagtccatgt tcgggggata tctcgagaaa gacgatcaaa
     2881 gccagagaca ggatcgcgaa ggacggtacg gcgcgcccga gattggcaag ggtgatcgct
     2941 atgaattcga atcgacgagc gtgaccgata tacaggccga cgggcaacgc caataagaag
     3001 gccgctatgg caggaacgta cgacagatag agatgtcgca ctagttgctc ggggatcgtt
     3061 tcggtggttg agaaccattg gatgagctcg ctcatccggt cgtcctcaca ctgcgggatc
     3121 tcgcccaagg tgcgagccac cgttcgactc gcaacaggac gaagtcggcc cccattgcaa
     3181 gaagcatcga cgcgaccgct cccaggatga tcgcggtggg aaaggtccgc gtgagaccga
     3241 gcaagatgaa gtaaccgaac ccacctcggc ccagcaccgc ggtgacggtc actagaccga
     3301 tcgtcgtcac ggttgcgatc cgaacgccgg ccaccaccgc cggaagagca agtggaagtt
     3361 caaccttcca caacaactgc cgtcgcgaat accccatccc tcgggctgcg tccctaacgt
     3421 cttcatccac ggagtcgagg cctgccacga tgttccggat caagatcaga agcgtgtaga
     3481 tgatcaacgg gataacggcg gtcgtcgtcg agaacgctcc agagaagggg atcaggagcg
     3541 cgaacagcgc cagacttggg atcgtgtaca tgactccggc ggcgagcgta atggggccgt
     3601 agacgcggcg gtgtctccac gagaacacgg ccacggggaa cgagatcagg aacccgaccc
     3661 cgaccgacag aagcgtcaag tagaagtgct cgcctagagc gaggtacacc tcttcggtgt
     3721 gatccaggat ccaatcccac ctgaagaagg ccggtgtcac gagacgatct ccttcgagat
     3781 ccgctccaag gtcaggatgc ccagatagcg ctgcccttcg ctggccacca cggcgacttg
     3841 ggtgcgcgac gtgacgatcg aatcgagggc ctgtcgcaac gtcgagtccc tggtgacata
     3901 ggcgctgaaa ggtttcggtc cagcttcgcc gggggtccga acgccctcca tcatttcggc
     3961 gttcacccac ccgaagaggt ggccgtcttc gaccacgctt acccagtcgt gatcgaactt
     4021 ctccatctgc cgccgagctt catccaccgt gtctgaagga gaaacgacgg ggccgtcttc
     4081 cacgtggatg tccgacacct tcaacaaag
//
LOCUS       DATMED010000270         3568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2530573, whole genome shotgun sequence.
ACCESSION   DATMED010000270 DATMED010000000
VERSION     DATMED010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530573"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VNP73_06890"
     CDS             complement(<1..1439)
                     /locus_tag="VNP73_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65683.1"
                     /translation="MKRHSAVVLVFVLILGVVAAGPGAEAGKRRKKAARMGLWNKPIA
                     ELPVFAKRPPKDTKESTKIPPAVSMAMLPDGRVVYWGGLESLEDGQYPLASDAPNQVQ
                     SSRTRILDLRGPQPIWRVPDPEDSGKHDLFCADQRLLADGRLIAVGGTVWESDPVENP
                     AGRPGGTGELFGSNATRFFDYGSEPGWKFNDEFMHYPRWYPTLITMPDGRLLVASGVE
                     RLLYNDKGINVHQTEIFDPATNKWTENGTEGEVSLPLFARMHLLPDGTIFYSGVGQMW
                     GPFGQAYDEAQWTTQKIYDPEGNAWAVSGMGAYGARSGAFSVLMPLKAPYKKASILIA
                     GGTLGTSPGSYAANDLTEIVTVEGGVSTSAPAAPLNNPRWYSSGVLLPNGHVLAFSGG
                     DKDEVIAPATEAPIRQAELWNGKVWKPLSTARRIRTYHNSAILLPDASVLVGGHAPIN
                     EGYGATGNPEPATGTSNLKDPSFEIFKPP"
     gene            1541..2503
                     /locus_tag="VNP73_06895"
     CDS             1541..2503
                     /locus_tag="VNP73_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65684.1"
                     /translation="MQEEFGPRAVAFGRAMAEISGHYRVALELYLAADQPAALLHASH
                     PLMEVLPVVYEDLLEHGSAADLARALGVAATAIRERRSAQDVNQAFESVTRLGVAALA
                     DVVGPAAMTLPYASSVVAALVRSAGSKYRLALDGGRVSLQPEYEDAYGFVRQAARMIE
                     GWPNAAEFQSGIEELEQLIPGPLPPDEPTPLDRVETVARGFRESLARAAGALLRETPT
                     PAEAVDRLEYRLALLADILDHGETGRAEKLVTRMYAEDLRDLEEPLTEAAPDIAADLG
                     ERLAGMRVAINRGASRADLAPEVRRIQGLLAEGLIRLKTSNQQG"
     gene            2586..>3568
                     /locus_tag="VNP73_06900"
     CDS             2586..>3568
                     /locus_tag="VNP73_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65685.1"
                     /translation="MRRLTISLFALLLLATVVVPAVAQEPPVPDPAFIKEVPGAKCEP
                     ETPYEKHGWKETASAAAGDKCKRIKFVYGPVTVKPGQNDVLIGPVTIEKPAYDGLITR
                     FKPDLVDASGVAPPINLVHLHHGTWLNAGDSYGNGPFFASGEEKTIGFWPKGYGMHVG
                     AQDQWLLLYMVHSAVSEPTEVWITYDLDFVADEDAEELGMVPTKPVWLDVQNDRIAKG
                     APSTGGNPVYNSQKGFGHHNKDLGIMECSWPKENCARHDVYGGVTPQQGKPIKLEGAD
                     YKVTEDMAGTLILMGGHLHPGGIRDEVSLVRNGKEKMIHISDAVYWDRKNP"
BASE COUNT          655 a         1115 c         1173 g          625 t
ORIGIN      
        1 taggggggct tgaagatctc gaacgacggg tctttgaggt tgctggtgcc ggtcgcaggc
       61 tctggattcc ccgtcgcgcc gtagccctca ttgatcggcg cgtgaccccc cacgaggaca
      121 ctcgcatccg gcagcaggat cgccgaattg tgatacgtcc ggatccgtcg agcggtcgac
      181 agtggcttcc acaccttgcc gttccacagc tcggcctggc ggatgggggc ctcagttgcg
      241 ggtgcgatca cctcgtcctt gtcgccaccc gagaatgcga ggacgtgacc gttgggaaga
      301 agaacgccgc tcgagtacca gcgcgggttg ttgagtgggg ccgctggagc gctggtcgaa
      361 acgccgccct cgaccgtgac gatctcagtc agatcgttcg ccgcgtagga gccgggggac
      421 gtgccgagtg ttcctccggc gatcaggatg ctcgctttct tgtatggagc cttcagaggc
      481 atcaacactg agaaagcacc gctgcgagcc ccgtacgcgc ccatgcccga cacggcccat
      541 gcgttgccct cagggtcgta gatcttctgc gtggtccact gcgcttcgtc gtaagcctgg
      601 ccgaacggcc cccacatctg cccgacgccc gagtagaaga tggtgccgtc gggaagcagg
      661 tgcatccgcg cgaacagcgg cagggagact tcgccttcgg tcccgttctc cgtccacttg
      721 ttggtcgcgg ggtcgaagat ctcggtctgg tgaacgttga tgcccttgtc gttgtagagg
      781 agacgctcga cgccggaggc gacgaggagt cgtccatccg gcatcgtgat caaagttggg
      841 taccagcggg ggtagtgcat gaactcgtcg ttgaacttcc atccaggctc ggatccgtag
      901 tcgaagaagc gggtcgcgtt cgagccgaat agctcgcccg tgccaccggg tcgaccggca
      961 gggttctcga cggggtcgga ttcccacacc gtcccgccca ctgctatcag tcgtccgtcg
     1021 gcaagcagcc gttgatccgc gcagaagaga tcgtgcttgc cggagtcttc ggggtccggc
     1081 actcgccata tgggctgtgg ccctctgagg tcgagtatcc gcgtccggct gctctgaacc
     1141 tgattgggcg cgtcggatgc gagcgggtac tgaccgtcct cgaggctctc gaggccgccc
     1201 cagtagacga cgcggccgtc ggggagcatg gccatcgaga cggcgggagg gatcttcgtg
     1261 gattccttcg tgtccttcgg ggggcgcttc gcgaacaccg gcagctcggc gatcggcttg
     1321 ttccacaggc ccatgcgcgc ggctttcttg cggcgcttgc cggcttcggc acccgggccg
     1381 gccgcgacca cgccgaggat caacacgaag accagtacga cagcactgtg tctcttcatc
     1441 acgcctcccg gggaccgatg ctcgatatat aagggattcg tccgcttcgc atcgaatcct
     1501 ccccctgcgg aggtcgatgt acccccaagg gagcaaacaa atgcaagaag agttcggccc
     1561 gcgcgccgtc gctttcgggc gggcgatggc agagatctcc gggcattacc gagtggcgct
     1621 cgagctctac ctcgcggcag accaaccagc ggccctgctt catgcgagcc accctttgat
     1681 ggaggtcttg ccggtcgtat acgaggacct cctggagcat ggatcggcgg cggacctggc
     1741 gagagcgctc ggcgtcgcgg ccaccgccat tagggaacgc aggtcggccc aagacgtgaa
     1801 ccaggccttc gagtcggtta cccgattggg agtcgcggct ttggcagacg ttgtaggccc
     1861 cgcagcgatg acgctgccgt acgcgtcttc ggtcgtcgcg gccctcgtcc ggtcggccgg
     1921 ctccaagtac cggctcgcgc tggatggggg ccgggtgtcg ttgcaaccgg agtacgagga
     1981 cgcttacgga ttcgtgcggc aagcggcgcg catgatcgaa ggctggccga acgcggccga
     2041 gtttcagtca gggatcgaag agctagagca actgatcccc ggccccctgc cccccgacga
     2101 accgacgccc ttggaccggg tcgagaccgt ggcgcgtggg ttccgagaat cgctggcgcg
     2161 ggcggccggg gcgctactac gcgagacccc cacaccggcc gaggcggtcg atcgactcga
     2221 gtaccgactc gcgcttctgg ccgacatcct cgatcacggg gagacggggc gtgccgagaa
     2281 gctcgtgacc cggatgtacg ccgaggatct ccgggacctc gaggagcccc tgaccgaggc
     2341 cgccccggat atagccgctg acctgggaga acgtctcgcc gggatgaggg tcgcgataaa
     2401 tcggggggct tccagggccg acctggcccc ggaagttcgc aggatccagg ggctcctcgc
     2461 tgaaggattg attcgcctaa aaacctcgaa ccagcaggga taggtgcaat cgtggtcgaa
     2521 tcgggtaatt agagctacac cgtaagcatt ccgagcgggc agcataaaaa cgacgggggt
     2581 ggaggatgcg ccggctgacg atctctctat ttgctttgct acttctagct accgtggtgg
     2641 tgccggccgt ggcgcaagaa ccgccggtgc ccgacccggc cttcatcaag gaggtcccgg
     2701 gagcaaagtg cgagcccgag accccttacg agaagcatgg gtggaaagag accgcgtcgg
     2761 cggcagcggg cgacaagtgc aagcggatca agttcgtcta cggacctgtc acggttaagc
     2821 cgggacagaa cgatgtcctg atcggccccg tcacgatcga gaagcctgcg tacgacggcc
     2881 tcatcactcg tttcaagccg gacctcgtcg acgcgagcgg tgtcgcacct ccgatcaacc
     2941 tcgtccacct ccaccacggc acgtggctca acgcgggcga ctcctacggc aacggaccgt
     3001 tcttcgcctc tggtgaagag aagacgatcg gcttctggcc gaagggctac ggcatgcacg
     3061 tcggcgcgca ggaccagtgg ctgctgctgt acatggtcca cagcgcggtt tccgagccga
     3121 ccgaagtgtg gatcacctac gacctcgact tcgtcgccga cgaggacgcc gaggagctcg
     3181 ggatggttcc caccaagccg gtgtggctcg acgttcagaa cgatcggatc gccaagggcg
     3241 ctccttccac cggcggtaac cccgtctaca actcgcagaa gggcttcggc caccacaaca
     3301 aggacctcgg gatcatggag tgctcctggc cgaaggagaa ctgcgcgcgc catgacgtgt
     3361 atggaggcgt cactccacaa caaggcaaac cgataaagct cgaaggtgcc gactacaagg
     3421 tcacggaaga catggccggg accctcatcc tgatgggcgg tcacttacat cccggcggca
     3481 taagagacga ggtgagcctc gtccgcaacg gcaaagagaa gatgatccat atctccgatg
     3541 ccgtctattg ggatcgcaag aacccgga
//
LOCUS       DATMED010000271         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4058862, whole genome shotgun sequence.
ACCESSION   DATMED010000271 DATMED010000000
VERSION     DATMED010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4058862"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VNP73_06905"
     CDS             complement(<1..798)
                     /locus_tag="VNP73_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWL65686.1"
                     /translation="MASPPEKVDASGAGLVAAPTYNPQVMTVVHPFSVDLPIDISVVL
                     PCLNEEAAIAGVIEEAWQGIEATGLRGEVIVVDNGSTDNSIKIAEGMAARVIHEPRKG
                     YGSAYLAGLAQARGEYIVMADSDGTYDLTQTKDLIDCLERGNDMVLGSRFRGHIHRGA
                     MPWTHRWIGNPVLTALLNILFGVRVSDAHCGLRCVRRSALPKLQLQAIGMEFASEMIL
                     KAGRRGLKIAEVPIEYRPRTGTSKLSSLRDGWRHLRLMLVYSSTLLFL"
     gene            857..>2201
                     /locus_tag="VNP73_06910"
     CDS             857..>2201
                     /locus_tag="VNP73_06910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWL65687.1"
                     /translation="MTADAATVRRTSLDPYEGSSWFFLLVRVHPFLVALIASGVLLRI
                     VTIVAYQPALLFHADAYGYLRIAEDLEPSPYRPVLYPILLRLAFPLHSLLPVVVVQHL
                     AGLAMGIAVYALLRRLGVGSFGGAVGSAPLFVDAYQLNIEQYVLSETLFMSLMVGSFF
                     LLSAARKPSARAVGVAGTLLGLAGITRVTGVVLIVPVLVYLWWRKVGLAKILWCVGGF
                     LLPLVIYAMWFLGHWGVFAITTKGSLILYGRVSPFANCAAAELPEYQRVLCDVRPPDE
                     RPGTEFYVWRTLNRYSPLLRLEPPPGRSEEEVLGQFARAIIVSQPLDYARVVVGDLAH
                     YLAPGRWEKPRDAQIEPWEFTDSFDFGPDDPEVIKVLQTTGDNLPQVIRENRGSPEPN
                     ELFGDQPTPELEINAPLARFLASYQAIVYTPGPLVALAILLGIIGAALPSPRGRRR"
BASE COUNT          374 a          713 c          657 g          457 t
ORIGIN      
        1 aaggaacaac aacgtcgagc tgtacacgag catcaaccgg aggtggcgcc acccatcccg
       61 gagactgctc agctttgacg tacctgtgcg cggcctgtac tcgatcggaa cctcggcgat
      121 cttcaatccg cgccgcccag ccttgaggat catctcggat gcgaactcca tcccgatggc
      181 ttgaagttgc agtttgggaa gagcagatct gcggacacat ctcaaaccgc agtgagcgtc
      241 ggagacgcgc acgccgaaca ggatgttgag caatgccgta aggaccgggt tgccgatcca
      301 gcggtgggtc catggcatgg cgccgcgatg gatatgcccg cggaagcgtg aaccgagaac
      361 catgtcgttt ccgcgctcga gacagtcgat cagatctttc gtctgggtca agtcgtaggt
      421 gccgtcgctg tcggccatca cgatgtattc gccgcgagcc tgggcgagac cggccagata
      481 tgcgctgccg taacctttgc gcggttcgtg gatgacacgg gccgccatcc cctcggcgat
      541 cttgatcgag ttatccgtgg agccgttgtc cacgacgatc acctcgcctc ggagcccggt
      601 cgcttcgatg ccctgccagg cttcctcgat cactccggct atcgccgcct cttcgttcag
      661 gcacggcaac acgaccgaga tatctatagg gaggtcgacg gagaaaggat gaacgaccgt
      721 catgacctgc ggattatagg tgggggccgc gaccagcccc gccccggacg catcgacctt
      781 ctcgggagga gacgccatta gtgtggcgaa ccgtaggcat gtttctgcca cgaccgcgag
      841 gaggacggcg gccgcgatga cagccgacgc tgcgacggtt cgaaggacgt cgctagaccc
      901 ctatgaaggc tcatcctggt tctttctcct cgtccgggtg catcccttcc tcgttgctct
      961 catcgcctcc ggggtcctgc tacggatcgt cacgatagtt gcgtaccaac cggctctcct
     1021 cttccacgca gatgcgtacg gttacctgcg catagccgag gacctcgagc cgtcgcccta
     1081 tcgccccgtg ctctacccga tcctcctgag actcgccttc cccttacaca gtcttctccc
     1141 ggttgtcgta gttcaacacc tcgccgggct tgcgatgggt atcgccgtct atgcccttct
     1201 gagacgcctc ggggtgggtt ccttcggggg cgcggtgggc tcggcccccc ttttcgtcga
     1261 tgcctatcaa ctgaacatcg agcagtacgt cctgtcggag accctgttca tgagtctcat
     1321 ggtgggctca ttcttcttac tgtcggcggc ccgtaagcct tctgcccgcg ccgtcggcgt
     1381 tgcgggaacg ctcctcggac tggccggtat cacgcgcgtc accggcgtcg tgttgatcgt
     1441 ccccgtcctc gtctatctgt ggtggcggaa ggtgggactc gcaaagatcc tgtggtgcgt
     1501 cggaggcttc cttctccccc tcgtcatcta cgcgatgtgg ttcctgggtc actggggggt
     1561 ctttgcgatc acgaccaaag gctcgttgat cctgtacggc agggtgtcgc cgttcgcgaa
     1621 ctgcgctgca gccgaactac cggagtacca acgcgtgttg tgcgacgtcc gaccacccga
     1681 cgaacgaccc ggcacggagt tctacgtgtg gagaacgctg aatcgatact cgccgttgct
     1741 tcggctcgag ccgcctccgg gtcgatcgga agaggaagtg ctcggccagt tcgcacgagc
     1801 gatcatcgtt tcgcagccac tcgactacgc gcgcgtcgtc gttggtgatc tggcgcacta
     1861 cctcgccccc gggcgttggg agaagccgcg cgatgcccag atagaaccgt gggagttcac
     1921 cgactcgttc gatttcggtc cggatgaccc cgaggtcatc aaggttcttc aaactaccgg
     1981 tgacaatctg cctcaggtga tccgcgagaa ccgagggtcg ccggaaccga acgagctgtt
     2041 cggcgaccaa cccacacccg aactcgagat caacgcgccg ctggcgcggt tcctcgcgtc
     2101 ttaccaagcg atcgtttaca cacccggtcc gctcgtggcg cttgccatct tgcttgggat
     2161 catcggagcg gcactcccct cgccccgcgg tcgccggcga t
//
LOCUS       DATMED010000272         3499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2530817, whole genome shotgun sequence.
ACCESSION   DATMED010000272 DATMED010000000
VERSION     DATMED010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530817"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNP73_06915"
     CDS             complement(<1..222)
                     /locus_tag="VNP73_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL65688.1"
                     /translation="MNEPVKVLLADDHPSTRAGVRFALEAADMVVCAEVRDAPSAVAA
                     AERERPDLCLLDIHMPGGGIVAAHAISLKV"
     gene            complement(219..1604)
                     /locus_tag="VNP73_06920"
     CDS             complement(219..1604)
                     /locus_tag="VNP73_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65689.1"
                     /translation="MAVRARSLTLGMAAVGATVTLLVALAPTLRFAYRNESLHIALDT
                     AGGVIAGIAALLLVGRVRQSELVRDAAAGLALGIFAFTNLFLSALPSALGAGEEPFWT
                     WTTLACRLMGAALFAISSLAGSRRFEDPGHLGTKLFAACAIAVGGVCALVVALGSGLP
                     PGVETFPPEASEHPHIVGHPVVLGSQVLATTLFVVATWGFTRLAATTGDPLLRWLGAA
                     SALGAFARVNYLLFPSIYSKYVYTGDILRLGYYILLAVGTVTEIGTYWRAREQAAVLE
                     ERRRMARDLHDGLAQELAFVVAWTRRLARRPELPFPADEVTAAAQRALDDSRMAIAAL
                     THTFDQSLSEAIAQVAYEIGHRENSKILLELEPQIDVTPHARQELIRIVREALTNACR
                     HSQAREIKVNLANPGRIRIAVIDDGIGFDPSAASFGFGVISMRERARSLGADFNIESD
                     SSAGTEVKVCL"
     gene            1912..3222
                     /locus_tag="VNP73_06925"
     CDS             1912..3222
                     /locus_tag="VNP73_06925"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HWL65690.1"
                     /translation="MRRTRGRHSLPKQNKGHLARTIQVAAIAAIISLLSAGIAFADNV
                     QNDVVAGGNDTITAGGSTTVNYRIIGNSSPGGDIGGCNVDATNSATLTISKPAAVSAT
                     PGSVQITECGPPGAKAVSFSSSTPGSHSITVSITGGKSGSLWNNQAAFTLTVNGAADS
                     APPSWECNPAAADGDWHDGEQSFACTASDPSGLKAGSPASFTLSTNVGSGNEDGNAST
                     GTQQLCDVHDNCTTAGPITGNKIDRKAPTNISFSGSISDGDSFYFGSVPANDMSCTAL
                     DGGSGFKDCSVLGYATTVGTHTLTATARDDVLNSSQSTISYTVLAWTLSGFYQPVDTT
                     VWNTVKGGSTVPLKFEVFAGSTELTSTSVVKSFTTKSVTCPGASAPTDEIEFVTTGGT
                     NLRYDTAGGQFVQNWQTPKKPGTCHSVTMETSDGSKISANFILK"
BASE COUNT          735 a         1082 c         1086 g          596 t
ORIGIN      
        1 caccttcagt gagatggcat gagctgcgac gatgcccccg cccggcatgt ggatgtcgag
       61 aaggcataag tcgggccgtt ctcgttccgc cgccgcaacc gccgaagggg cgtcccgaac
      121 ctcggcacag accaccatgt ccgcggcttc gagcgcgaac cgcacgcccg ctcgggtgga
      181 gggatgatcg tcggcgagca gcaccttgac cggctcattc acaagcagac cttgacctcc
      241 gttccggcac tagagtcaga ctcgatattg aaatcggctc cgagggaacg cgcccgctct
      301 cgcatactta tcacgccgaa gccaaagctt gcggccgagg gatcgaaacc gatcccgtca
      361 tcgatgacgg cgatccttat ccttcctggg ttcgcgagat tgacttttat ttcccgcgcc
      421 tgagaatggc ggcaggcgtt cgttagcgct tcgcggacga tccggatcag ctcctggcgc
      481 gcgtgcggtg tgacgtcaat ttggggctca agctctagca agatcttcga gttctcgcgg
      541 tgcccaatct cgtatgcgac ctgggcgatt gcttccgaca gcgactgatc aaatgtgtga
      601 gtaagcgctg cgatcgccat gcgtgagtca tcgagggcac gctgggccgc cgcggtcact
      661 tcgtcagcag ggaagggcaa ttcgggacga cgagcgaggc gccgggtcca cgccactacg
      721 aacgcaagtt cctgtgcgag cccgtcgtgg agatcccgcg ccatgcgtct gcgttcttcg
      781 aggacggctg cctgctcccg ggcacgccaa tacgttccga tctcggtaac cgtaccgacg
      841 gccaggagga tgtagtagcc gaggcgcagg atgtcgcctg tataaacgta tttcgagtag
      901 atcgacggga acaggaggta attgacgcgt gcgaacgcgc ccagggccga cgctgctccg
      961 agccagcgca ggagtggatc gccggttgtg gcggcgagtc gagtaaagcc ccaggtcgcc
     1021 accacgaaca gcgttgtcgc cagtacctgg ctgccgagca ctacgggatg gccgacgatg
     1081 tgggggtgtt cggacgcctc tggcggaaaa gtttcgaccc ccggggggag cccggaccca
     1141 agggccacaa ccaaggcgca aacgcctccg accgcgatcg cgcatgccgc gaagagcttg
     1201 gttccgaggt gtccaggatc ctcgaagcgg cgcgagccgg ccagggacga aatcgcgaac
     1261 agcgcggcac ccattagtcg gcaggcgagg gtggtccaag tccaaaacgg ctcctcgcca
     1321 gctcccaggg cagatggtag cgccgagagg aagagatttg tgaacgcgaa gatgccgagg
     1381 gccaaccctg cggcggcgtc tctgacgagt tcactttgcc ggactcgtcc gacgagtagc
     1441 agggctgcga ttcctgcgat gacacctccg gcggtgtcga gggcgatgtg gagggattcg
     1501 ttgcggtagg cgaatcggag cgtgggggcc aatgcgacca aaagagtgac cgtcgctcca
     1561 acggccgcca tccccagcgt gagagaacga gctcgaaccg ccaaagtgcg aatgcgaccc
     1621 ctttctgccg gtcggtgacc tgctagtaga attatgggtc cttctaccag cagattcggc
     1681 gcccctcgtg cccgcctgcc taggcagagc gttgaagaaa ttcaacaccc atacatccga
     1741 acgggccata ccaattctta cctatcacgc ctagtttcga ttgatagaga cggagcttcg
     1801 atccgtgggc ttttacatgg tcgcctgggc cttcggggag ccagtggcga aatcgacgca
     1861 ggagaaaatc gcgcgggcgc aagcgtgccc gccaaagcag aggagaagcg aatgaggcgg
     1921 acgaggggtc ggcacagcct tcccaagcaa aacaagggcc acctggctcg gaccattcaa
     1981 gtggccgcga tagctgcgat catcagcctg ctgtcggcag ggatcgcctt cgccgacaac
     2041 gtacagaacg acgttgtcgc cggcgggaac gacaccatca ctgctggcgg ttcgacgact
     2101 gtgaactaca ggatcatcgg taacagctca ccgggcggtg atattggcgg ttgtaacgtc
     2161 gatgcgacga actcggccac gctgactatc agcaagcctg cagccgtttc ggcaacgccg
     2221 gggtccgttc agatcaccga gtgtggccct ccgggtgcga aggccgttag cttctcctcc
     2281 tcgacccccg gtagccactc gatcaccgtg tccatcacgg gaggcaagtc cggaagcttg
     2341 tggaacaacc aggcggcctt cacgctcacg gtcaacggag ctgcagactc ggcacctccg
     2401 tcctgggaat gcaaccctgc ggctgcggac ggtgactggc atgacggcga gcagagcttc
     2461 gcatgcaccg ccagcgaccc ctcgggactc aaggccggtt cacctgccag tttcaccctc
     2521 tccaccaatg tgggatcggg caacgaggac ggcaacgcct cgaccggaac ccagcagctg
     2581 tgcgacgttc acgacaactg cacgaccgcg ggaccgatca ccggcaacaa gatcgaccgc
     2641 aaggctccga ccaacatctc gttctccgga tcgattagcg atggtgacag cttctacttc
     2701 ggaagcgtcc ctgcgaacga catgagttgc accgcgctgg atggtggctc agggttcaag
     2761 gactgctcgg tcttgggata cgccacgacc gtcggtacgc acacgttgac cgctacggca
     2821 cgggatgacg tcctgaactc gtcgcaatcc acaatcagct acacggtgct cgcatggacg
     2881 ctctcgggct tctaccagcc cgtcgacacg accgtctgga acaccgttaa gggcggtagc
     2941 acggttcccc tcaagttcga ggtcttcgca gggtcgacgg agctcaccag cacttccgtc
     3001 gtcaagagct tcacgaccaa gtcggttaca tgccccggcg cgagcgcccc gacagacgag
     3061 atcgagttcg tgaccacagg tggtacgaac ctgcgctacg acaccgcggg cggacagttc
     3121 gtccagaact ggcagacgcc caagaagccg ggcacctgtc acagcgtcac gatggagacg
     3181 agcgacggct ccaagatcag cgccaacttc attctgaaat agacccacgc gaggcgacgc
     3241 tctagtttcg atagtcatcg gcggtggaca gaggagcatg agtatgcaga ggccccggaa
     3301 accggggcct ctgctcgcgt gaattcatgc ggaccaagaa ccggtacgtg aacaactgac
     3361 caacagcttc cctgtcagta cagcactcgc ggcgtttcac ggacgagctc ggcctgcacc
     3421 aagaaagtcc agccaggggt cgcgagttca aatccacaac ctgacgcggg tgtaggagac
     3481 tctggcggta cggaacaca
//
LOCUS       DATMED010000273         3562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5217506, whole genome shotgun sequence.
ACCESSION   DATMED010000273 DATMED010000000
VERSION     DATMED010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5217506"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VNP73_06930"
     CDS             <1..469
                     /locus_tag="VNP73_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HWL65691.1"
                     /translation="AVFEEVGPDAPAWTWHGENDVAFWIRRMAQETAVHCWDAENAIG
                     SPTEIDAELAADGIDEFVDKFIALDEIAYEGPSGTVHLHCEDTEDRWTVSLDPGKVPT
                     FGRGPADADAVVRAPAHNLLLWIWRRLGSSSVEVSGAPALADAFWYYAEGPGQ"
     gene            470..1018
                     /locus_tag="VNP73_06935"
     CDS             470..1018
                     /locus_tag="VNP73_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWL65692.1"
                     /translation="MGFYVALLRGINVGGKNMIRMPALKECFEAGGFEDVATYIQSGN
                     VLFRASGGASALADRIEKMLSKTFTHYQATVVVRSRTQMRTTIEKAPKGFGTKPKTYL
                     SDAIFLKEPLTAAAALKQVPTKEGVDEIHAGRGVLYMQRLASKASSSRLSRVAALPIY
                     KKMTIRSWNTTTRLARMMEELG"
     gene            complement(1015..2049)
                     /locus_tag="VNP73_06940"
     CDS             complement(1015..2049)
                     /locus_tag="VNP73_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HWL65693.1"
                     /translation="MNPAIEGRLRKQARSCAALGSPLYAHLLDRAADDAANGGIVAEA
                     MEPWADAPSGTAIGLKLMGSVHRLVLTGELPELAAFYPSAGGAQPVEGSELPFFAALG
                     DKAQEISQLLHLPVQTNEVGRSAALLGGFLEVAHLPLRVLEVGSSAGLNLRWDHYRYE
                     HDGIAWGPTDSPVTFAPYESAPNLGLEAQVVARIGCDPAPIDASTDAGRLTLSSYVWA
                     DQAKRWQLLQSALEVATRVPARIERVGAGDWLERVLAEPAPGTSTVVFHSIVTQYMTP
                     AERERVVAVITEAGELATPNAPLAWLRMEPPEERGDDLAHVHLTRWPGGETRLVARAG
                     YHGRPVIWLA"
     gene            2085..3389
                     /locus_tag="VNP73_06945"
     CDS             2085..3389
                     /locus_tag="VNP73_06945"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWL65694.1"
                     /translation="MAMVFIAGGGYVGMYSALNLKRRAPTGTEITLVAPESNMTYQPF
                     LPEAASGSIEPRHVVIPLRTVLKGVRLITGRVTAIHHPAKRATVVPVEGDAYEIDYDI
                     LVLGVGSLSRVLPVPGLAEYAVGFKTVAEAIYLRNQVLSRMDAAESTPDEKIRKRALT
                     FTFVGGGYAGVEALAELEDLARDACRYYSTVTRDDMRWVLVEAADGILPEIGKGLGDY
                     ALERLRARGIEVHLNTRLESAQDGVMKLSTGEAFESETLVWTTGVKAHPLVSTVAPAP
                     DKAGRIEVDEYLRVKGLDDVWAAGDCAAVPDVTTGGICPPTAQHALRQARRLGRNVAA
                     VLKKQEPQPFRYRNRGGLVSLGRYKGVARIPGMRIRGFPAWFLHRTYHVFMIPTTNRK
                     ARVLADWTVALFFKRDVVQLGSLAHPRAAFSEATEGWQRPGP"
     gene            complement(3349..>3562)
                     /locus_tag="VNP73_06950"
     CDS             complement(3349..>3562)
                     /locus_tag="VNP73_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65695.1"
                     /translation="EMGRTLDIGGPKSIPAGVVKIVSSYAAMMAATNLRVSNAKIKNE
                     LGWMPQFPSCREGAKDLAAATPQSLR"
BASE COUNT          664 a         1138 c         1133 g          627 t
ORIGIN      
        1 cgccgttttt gaggaggtcg gccccgacgc gcccgcgtgg acgtggcacg gtgagaacga
       61 cgttgcgttc tggatcaggc gcatggccca ggagaccgcc gttcactgct gggatgcgga
      121 gaacgcgatc ggttcaccca ccgagatcga tgcagagctc gccgctgatg ggatcgacga
      181 gttcgtagac aagttcatcg ccctcgacga gatcgcctac gaaggtccga gcgggacggt
      241 gcacctgcac tgcgaggaca ccgaggaccg atggacggtg tccctggatc ccgggaaagt
      301 tccgacattc gggagaggcc ccgccgacgc cgatgcggtc gttcgtgctc cggctcacaa
      361 cctgctcctg tggatctggc gacggctcgg ctcatcgtcc gtcgaggtgt ccggcgcccc
      421 tgcgttggcc gatgcgttct ggtactacgc cgagggcccc ggtcaataga tgggcttcta
      481 cgtagcgctt ctgcgcggga tcaacgttgg cggcaagaac atgatcagga tgcccgctct
      541 gaaggaatgt ttcgaagcgg gagggttcga ggacgtcgcc acctatatcc agagcggcaa
      601 cgttttgttc cgggcttccg gtggcgcctc ggccttggcc gaccggatcg aaaagatgct
      661 cagcaagacc ttcactcact accaagcgac ggttgtggtt cgaagccgca cgcagatgcg
      721 aaccaccatc gagaaggcac ccaagggatt cggaacaaaa cccaagacct acctctccga
      781 cgccattttc ctgaaagagc cgctaactgc agccgccgcg ctcaagcagg tgccgacgaa
      841 agagggcgtt gacgagatcc acgccggccg cggggtgctc tatatgcaaa ggctcgccag
      901 caaagcttcc agcagccgct tgagcagggt cgccgcattg cccatctaca agaaaatgac
      961 catccgcagc tggaacacga ctacccgtct cgcacggatg atggaagagc tcggctaagc
     1021 gagccatatg acgggccggc cgtgataccc ggctcgagcg accaagcgag tctcgccacc
     1081 cggccaccgt gtcagatgaa cgtgggcgag gtcgtccccg cgctcctcgg gaggctccat
     1141 ccgcaaccac gccagcggag cgttcggcgt cgcgagctcc ccggcctccg tgatcactgc
     1201 gacgacccgc tcgcgctcgg ccggcgtcat gtactgggtc acgatcgagt ggaacacgac
     1261 cgtgctggtg ccgggagcgg gctcggccag tactcgctcg agccaatcgc cggctccgac
     1321 gcgctcgatc cgcgcgggga cacgagttgc cacttctagt gctgactgaa gcaactgcca
     1381 tctcttagct tggtccgccc acacatacga agagagtgtg agtcgacctg catcggtcga
     1441 agcgtcgatc ggcgcgggat cgcagccgat tcgcgcaacg acttgagctt caagcccgag
     1501 gttgggtgcg gattcgtaag gcgcgaacgt cacgggagag tcggtcggcc cccatgcgat
     1561 cccgtcgtgc tcgtagcggt agtgatccca cctgaggttc agccctgcgc tgctgccaac
     1621 ctcgaggacc cttaatggaa ggtgtgcaac ctcgaggaag ccgccgagca gtgcagcgga
     1681 acgcccgacc tcattcgttt gaacgggcag gtgaagaagt tgcgagatct cttgtgcttt
     1741 gtctcctagc gccgcgaaga atggaagttc agatccttcg acgggttgtg cccctccggc
     1801 ggagggatag aacgcggcca gctccgggag ctcgccggtt agaaccagac ggtgaaccga
     1861 acccatcaac ttcaatccga tcgccgttcc cgaaggtgcg tcggcccacg gctccatcgc
     1921 ctccgcgacg atgccgccgt ttgccgcgtc gtctgccgcc cggtcgagaa gatgcgcgta
     1981 cagaggtgac cccaaagccg cgcaagaacg cgcttgcttc cgcaatcgcc cttcgatggc
     2041 cgggttcacc ccgcgaggct agtcacctct gggacactta gcccatggca atggtgttta
     2101 tcgcaggcgg gggttacgtg gggatgtact cggccctcaa tctgaagcga cgggccccca
     2161 cggggaccga gatcacgctc gtggcccccg agagcaacat gacctaccag cccttcctgc
     2221 cggaggccgc ctcaggcagc atcgaacccc gccacgtggt gatcccgctc cggaccgtcc
     2281 tgaaaggggt ccgtctgatc accggccgtg tcacggcgat ccaccaccct gcgaagcggg
     2341 cgacggtcgt tccggtcgag ggcgatgcct acgagatcga ctacgacatc ctcgtcctgg
     2401 gggtggggtc gttgtcccga gtcttgccgg tgccgggtct tgcggagtac gcggtgggct
     2461 tcaagaccgt cgccgaggcc atctatctaa ggaaccaggt tttgtctcgg atggatgcgg
     2521 ccgagtcgac ccccgacgaa aagatccgga agcgtgccct caccttcacg ttcgtgggtg
     2581 gcggctacgc cggggtcgaa gctctcgccg agctcgagga cctagcccgc gacgcgtgtc
     2641 gttattactc gacggttact cgcgatgaca tgcgctgggt tctcgtcgaa gcggccgacg
     2701 ggatcctccc cgagatcggc aagggcctgg gcgattacgc gttggaacgt ctgcgagcgc
     2761 gaggcatcga agtccaccta aacacgcgac tagagagcgc gcaggacgga gtcatgaagt
     2821 tgtcgaccgg agaagcattc gagtccgaaa ctctcgtgtg gacgacagga gtcaaagctc
     2881 accctctcgt ctcgaccgtc gcgccggccc cggacaaagc aggtcgcatc gaggtcgacg
     2941 agtacctgcg cgtgaaaggt ctggacgacg tgtgggccgc gggcgactgc gcggcggtcc
     3001 cggatgtcac gacgggaggt atctgtccgc cgaccgcgca acacgcctta cggcaagcac
     3061 gtcgcttggg ccgcaacgtg gccgcggtcc tcaagaagca agagccgcaa ccgttcagat
     3121 accggaaccg aggtgggttg gtctcactcg gtcgctacaa gggagtggca cgcatccccg
     3181 ggatgcgcat ccggggtttc cccgcttggt tcttgcatcg cacgtaccac gttttcatga
     3241 tcccgacgac caatcgcaaa gcccgtgtgc tggccgattg gacggttgcg ttgttcttca
     3301 agcgcgacgt agtgcaactc ggttcgctgg cccacccccg cgcggcgttc agcgaagcga
     3361 ctgaggggtg gcagcggcca ggtccttagc cccctcgcga cagctcggga actgcggcat
     3421 ccagcccagc tcgtttttga tcttggcgtt ggacacgcgc aggttcgtag cagccatcat
     3481 ggccgcgtag ctgctcacga tcttgacaac cccggccggg atcgacttcg gaccaccgat
     3541 gtccaacgtc cggcccatct cg
//
LOCUS       DATMED010000274         2915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_968621, whole genome shotgun sequence.
ACCESSION   DATMED010000274 DATMED010000000
VERSION     DATMED010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968621"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VNP73_06955"
     CDS             <1..293
                     /locus_tag="VNP73_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65696.1"
                     /translation="ERLEIAGDTFDWFGATDGELRHKFLILNAYYLPGGHQQDLYPTI
                     SPVNSFRVVFNEYLGTDLPLLEDRSYAHRSTADLYNFIEVTERIRDMPADRN"
     gene            complement(290..>2915)
                     /locus_tag="VNP73_06960"
     CDS             complement(290..>2915)
                     /locus_tag="VNP73_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWL65697.1"
                     /translation="GLLAGIRERAAKAVTDERMSGWVAATSQGYRSPSNGIGETVDQI
                     FGFLLGACTIRSGFWGEIVLEITDQFLATPPAPRKPNWDYFRSQDGMWRPPGWLLPAT
                     AGNDPERRDIAFAVLRSLVDVFEGLEPLEARREALARLFDQRAADPSLHQADRTVPPE
                     EMPDDWAAAADPETLATLPELSGPAGYLKWTVEGLTAVHNHLLTVASGGTAFSEMLAN
                     LLLAAGIQRTPAELTLAIGDDELNMAEDWYRRLQPNFVPETWRDVTAGWLVRATIKGK
                     LAACRAWLDMAMRFTGAVNGAPGRAMHPPVTVWVPIVTFERGVRSLLATRPLVNPLSD
                     QFRTTASTTPVVVEAPVIDVGVVGQPELAAALREEAASAGPIRLLIAGPPGTGRGTAI
                     DALVELLKTRGIYQRPVFLPAALVTDKTTAGAIEQMRYEIGRCDNVALLVLQGLDEML
                     TSGESGEELAAELHLSLDSRPTLNIVALCDPGGDAEVFAADPILAHEFRIVRTVDFDE
                     ITFAELFRRKIQSLGAETDEATIATAGRMLVGTRPFRNLRNGHLVSVFATDAVARARI
                     RTDEKPTLVTTEDLPPDITGTGTELGDPMVELDSLIGLEEIKEEVKLLAAEAGAERAR
                     REAGMVVSPPTRHIAFTGNPGTAKTTVARLLARIYNSLGLLSGGHLVEVSRVDLIGRY
                     IGQTAPLVRAAVERAMGGLLFIDEAYALSPPGSDNDFGKEAIATLVKLMEDNRDDLVV
                     VVAGYEADMDRFLQSNPGLASRFSRRLRFPDYDDAQLLDIFRMMIEGSGVVVGDGVED
                     ALGRLLQAIPRGPSFGNGRLVRTIFERAMGKQALRVTLAGATADPAALRLLLPQDMPD
                     LRSLGIQEAAKPETGQYL"
BASE COUNT          499 a          954 c          935 g          527 t
ORIGIN      
        1 ccgagcgact cgagatcgcc ggcgatacct tcgattggtt cggtgccacc gatggcgagc
       61 tgcgccacaa attcctgatc ctcaacgcgt actacttgcc cgggggccac caacaggacc
      121 tgtatccgac gatctcaccg gtgaacagct tccgtgtcgt cttcaacgag tacctcggaa
      181 ccgatctacc gctactcgag gatcgaagct acgcgcaccg cagcacggca gatctctaca
      241 atttcatcga agtcaccgaa cggatccgcg acatgccggc ggaccggaac tagaggtact
      301 gaccggtctc cggtttggcc gcctcctgga tcccgaggga tctcagatcg ggcatgtcct
      361 gcgggaggag caaacgcagg gcggccggat cggcagtggc gccggccagg gtgaccctga
      421 gcgcctgttt gcccatcgct ctttcgaaga tcgttcgaac caaacgcccg ttgccgaagc
      481 tcggcccccg ggggatcgct tgcaggagcc ggcccagggc gtcctcgaca ccatcgccga
      541 ccacgacacc ggatccttcg atcatcatcc tgaagatgtc taggagttgc gcgtcgtcgt
      601 agtcggggaa tcgcagacgg cgagagaaac gcgacgccag cccggggttc gactgaagga
      661 agcgatccat gtcggcttcg taaccggcga ccacgacgac cagatcgtcg cggttgtcct
      721 ccatgagctt gacgagtgtc gcgatcgctt ccttcccgaa gtcgttatcg cttcccgggg
      781 gcgacagcgc gtacgcctcg tcgatgaaga gaagaccgcc catggctcgt tcgactgcgg
      841 ctcgcaccag cggggcggtt tggccgatgt atcgaccgat caggtcgact cgggagacct
      901 cgacgagatg accacccgac agcaacccca gagagttgta gatgcgggcg aggagcctag
      961 ccaccgtcgt cttcgccgta ccagggttgc cggtgaatgc gatgtgtcgg gtcgggggtg
     1021 agacgaccat gccggcctcc cgccgcgctc gttctgcgcc ggcctcggcg gcgaggagct
     1081 ttacttcttc cttaatctct tcgaggccga tcaggctgtc gagctccacc atgggatcgc
     1141 cgagttcggt tccggtgccc gtgatgtcgg gaggcagatc ctcggtcgtg accagtgtcg
     1201 gcttctcgtc cgtccggatg cgcgcacgcg ccaccgcatc ggttgcgaac acggatacga
     1261 gatggccgtt gcggaggttg cggaaggggc gagttccgac gagcatccgc ccggcggtcg
     1321 cgatcgtggc ctcgtccgtt tccgctccca gactttggat ctttctacgg aagagctcgg
     1381 cgaacgtgat ctcgtcaaag tccacggttc gcacgatccg aaattcgtga gcgaggatcg
     1441 gatccgcagc gaagacctcc gcgtcgccgc ccggatcgca cagcgcaacg atgttgagag
     1501 tgggtcgcga atcaagtgag aggtggagct cggccgcgag ttcctccccc gactcgcccg
     1561 acgtcagcat ctcgtcgagt ccttgaagga cgagcaaagc cacgttgtcg caccgcccga
     1621 tctcgtagcg catctgctcg atggctccag ccgtcgtctt gtcggtcacc agtgcggccg
     1681 gcaagaacac gggacgttgg tagatccctc tggtcttcag cagttcgacc agcgcgtcga
     1741 tggcggtgcc tcttcccgtc ccggggggtc ccgcgatcaa caggcggatc ggcccggccg
     1801 aagcggcctc ctcgcgcagc gcggctgcca gctctggttg accgaccact cccacgtcta
     1861 tcaccggggc ctctaccacg accggtgtcg tcgacgcggt cgtgcggaac tgatcgctca
     1921 gcggattcac cagcggacgc gttgcaagca gcgagcgaac gccacgctcg aaggtgacga
     1981 tcggaaccca gaccgtgacc ggcgggtgca tcgcccgccc cggcgctccg ttcaccgcac
     2041 ctgtgaaacg catggccatg tcgagccacg cgcggcaggc ggcgagcttg cctttgatgg
     2101 ttgctcgaac gagccacccg gcggttacgt cgcgccatgt ttcgggaacg aagttgggct
     2161 ggagcctgcg gtaccagtct tcggccatgt tgagctcgtc gtcaccgatc gccagcgtga
     2221 gctctgcggg ggtgcgttgg atcccggccg cgaggagaag gttggcgagc atctccgaga
     2281 atgccgttcc acccgaggcg acggttagta ggtggttgtg cacggccgta aggccttcta
     2341 cggtccactt gaggtagccg gccgggccgc tcaactcggg cagcgtcgcc agggtttcgg
     2401 gatctgccgc tgcggcccaa tcatccggca tctcttccgg cggcaccgtc ctgtcggcct
     2461 ggtgcagcga tggatcggcg gctcgctggt cgaatagccg ggccagcgcc tcgcggcgcg
     2521 cttcgagcgg ttcgagcccc tcgaacacgt cgaccaggga acgcaggact gcgaacgcga
     2581 tgtcgcggcg ctcgggatcg ttgcctgcag tggccggcag cagccaacct ggcggcctcc
     2641 acatcccgtc ctgacttcgg aagtaatccc agttgggctt ccgcggggcc ggtggcgtag
     2701 caaggaactg atccgtgatc tccagcacga tctcgcccca aaacccggac cggatcgtgc
     2761 aagctccgag gaggaaaccg aagatctggt cgaccgtttc cccgatcccg ttgctgggtg
     2821 agcgatagcc ctggcttgtc gccgccaccc atccggacat gcgttcgtcc gtgacggcct
     2881 tcgctgctcg ctcgcggatc ccggccaaca gaccg
//
LOCUS       DATMED010000275         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1370061, whole genome shotgun sequence.
ACCESSION   DATMED010000275 DATMED010000000
VERSION     DATMED010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370061"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..745)
                     /locus_tag="VNP73_06965"
     CDS             complement(125..745)
                     /locus_tag="VNP73_06965"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWL65698.1"
                     /translation="MDRLPAIAALVLWAAWGVLTLGFRVAIHVKETGTTGLHGIRAGA
                     SASEWAAGILIVAATVAGIAAPILQLTGVAPLLWVNPIVRVAGGIGALIGVAATFGAQ
                     IAMGRSWRIGVDPGERTDLVTEGPFAVVRNPIFTTMIVTMVGLALLVANGVAAAAAVG
                     MFVAIELQVRSVEEPYLTEVHGDAYLGYLTRVGRFVPGLGTRRSEA"
     gene            765..1679
                     /locus_tag="VNP73_06970"
     CDS             765..1679
                     /locus_tag="VNP73_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HWL65699.1"
                     /translation="MDDSLTLLVDAPSLIYRALFSTPDSISTPQGTPINAAYGFIRML
                     ARLVADHDPDYIACATDEDWRPEWRVELIPEYKAARAEPGSAQEEAEDLLAPQVPVAY
                     DLLEQTGIRVVGHEGFEAEDIIGTLAVRAPGKVLIFSGDRDLFQLVEDPHTVVLYPIR
                     GVSKLDFVDESYIEGKYGIPGRAYKDYAILRGDASDGLPGVRGIGEKLASALVAKHGS
                     LDKIIEAAETDAKGLPLQKVKRDLDYVKRAEQVVTIQTDIPIPEVDLTRPRQIEAKEI
                     LAAAGDHGLASPVKSLLQALAGGTGVGG"
     gene            complement(1641..2285)
                     /locus_tag="VNP73_06975"
     CDS             complement(1641..2285)
                     /locus_tag="VNP73_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018551280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65700.1"
                     /translation="MSIVLPIPADARPRRFVQLFAGLLLFGFSTGLLVEANLGLDPWD
                     VLHQGAQRRTGATIGTATIAISVLVLFLWIPLRQRPGFGTLSNAIVVGLALDATVFLM
                     PRPETLLVRGATLAAAIALNGVATGLYIGAGMGPGPRDGLMTGLAARGHSIRVVRTAI
                     EVTVLAVGWRLGGTVGIGTVAYAVLIGPLAHVFIPLFTVGGGSPTHPGAPGKGL"
     gene            complement(2321..>2784)
                     /locus_tag="VNP73_06980"
     CDS             complement(2321..>2784)
                     /locus_tag="VNP73_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HWL65701.1"
                     /translation="WPGERWLDVGRLDLLQPIMGARLDICVEKGFDAVDVDNVDGYTQ
                     DSGFDISWDEQIAYNRWLAQAAEDRGLAAGLKNDLDQVSELVGDYEFSVNEQCFLYRE
                     CKLLKPFIDANKPVLNIEYELERRRFCPRAREMRFSSIRKRMSLRAWRRPC"
BASE COUNT          569 a          946 c          824 g          445 t
ORIGIN      
        1 tcgtctccgg ctccgggagt gacgttcgca cccgtcaaac gagcacgctg actcgaggtg
       61 tgggcccaca cggttcccgc aacgaggacg atcagaagca ctgccgcgag cacaccgagc
      121 ttcctcatgc ctcgctcctc cttgtcccca accccggtac gaagcgcccc actctagtca
      181 ggtagccgag atacgcatca ccgtgcactt cggtcagata gggctcctcg acgctcctga
      241 cctgcagttc gatcgccacg aacatcccga cggcggcggc agcggccacc ccgttcgcta
      301 cgagaagagc cagtcccacc atcgtcacga tcatcgtagt gaagatcgga ttcctcacga
      361 cggcgaaagg accttcggtt accagatcgg tccgctcgcc ggggtccacc cctatccgcc
      421 acgagcgacc catcgcgatc tgtgccccga aagtcgcagc cactccgatc agagctccga
      481 tcccgccggc gacgcgaacg atcgggttga cccacagcag tggtgcaacc ccggtgagtt
      541 gaaggatcgg cgcagcgata cccgccaccg tcgcggccac gatcaggatc ccggccgccc
      601 actccgatgc cgatgccccg gcccggatcc cgtgcagccc cgtcgtgccg gtttccttga
      661 cgtgtatcgc aacgcgaaat cccaaggtca gcacacccca ggcggcccac aggaccagag
      721 cggcgatggc gggcaagcga tccacgaggt gaaactatgc ggtgatggac gactccctca
      781 ctctgttggt cgatgccccg agcctgatct acagagcatt gttctcgacc cccgactcga
      841 tctcgacccc gcagggcacg ccgatcaatg ccgcgtacgg attcatccgc atgctggcgc
      901 gcctcgtcgc cgaccacgac ccggactaca tcgcgtgcgc gacggacgaa gactggcgac
      961 ccgagtggcg cgtcgagttg atcccggagt acaaggcagc acgcgccgag ccgggcagcg
     1021 cgcaggaaga ggctgaagat cttctagctc cgcaggtgcc ggtcgcgtac gaccttctcg
     1081 aacagactgg gatcagagtc gtcggacatg aaggattcga agccgaggac atcatcggga
     1141 cgcttgcggt cagagccccc ggcaaggtcc tgatcttctc gggagaccgc gacctgttcc
     1201 agctcgtcga agatcctcac accgtcgtcc tgtatccgat ccgcggcgtc agcaagctcg
     1261 acttcgtcga cgagagttat atcgaaggca agtacgggat cccagggcgt gcctacaagg
     1321 actacgccat cctgcgcggt gatgcatccg acgggctgcc cggcgtccga ggcatcggcg
     1381 agaaactggc atccgcgtta gtggctaagc acggaagtct cgacaagatc atcgaagccg
     1441 ccgagaccga cgcgaagggt ctcccgctgc agaaggtcaa gcgcgatctc gattacgtca
     1501 aacgtgccga acaggtggta actatccaga ccgacatccc gatcccagaa gtcgatctca
     1561 cccggccgcg tcagatcgaa gctaaggaga tcctggccgc agccggcgac cacggactgg
     1621 catcacccgt caaaagcctg ctacaagccc ttgccggggg caccggggtg ggtgggtgat
     1681 cctccgccca ccgtgaagag cgggatgaaa acgtgggcca aagggccgat cagcacggcg
     1741 taagcaaccg tgccgatgcc gacggttcca ccgagtcgcc accccaccgc caggacggtc
     1801 acctcaatgg cggtccggac aaccctgatc gagtggccgc gggcggcaag cccggtcatc
     1861 agcccgtccc gggggcctgg acccatcccg gccccgatgt agaggccggt cgcaaccccg
     1921 ttcagagcga tcgccgcggc cagtgtcgct ccgcgtacca gcaacgtttc gggccggggc
     1981 atcaggaaca cagttgcgtc gagagcgagc cctacgacga tcgcgttgct caacgtcccg
     2041 aaccccggtc gttgacgcaa agggatccac agaaacagca cgaggaccga gatcgcgatc
     2101 gtcgccgtcc ctatcgtcgc tcccgttctg cgttgagctc cctgatgcaa cacgtcccat
     2161 gggtcaaggc ccaggttggc ctctaccaat agacccgtac tgaagccgaa gagaagcagc
     2221 ccggcgaaca gctggacgaa acgtcgtggg cgcgcgtcgg cagggatagg taagacgatg
     2281 gacatcgaac gagggtagag gaccggtcgc agtcagccgg tcagcagggc ctgcgccacg
     2341 cacgcagact catccgcttc ctgatcgagc tgaagcgcat ctcgcgagcc cgggggcaga
     2401 accggcgacg ctcaagttcg tattcgatgt tgagaaccgg cttgttcgca tctatgaacg
     2461 gcttgagcaa cttgcattcg cggtagagga aacactgttc gttgaccgag aactcgtagt
     2521 cccccacaag ctcagacacc tgatcgaggt cgttcttcag tcccgcggcc agaccgcgat
     2581 cctccgcagc ctgcgccagc cacctgttgt aggcgatctg ttcgtcccac gagatgtcga
     2641 acccgctgtc ctgcgtatag ccgtcgacgt tgtcgacgtc gaccgcgtcg aaacctttct
     2701 ccacgcagat gtccagtcgc gcccccatga tcggctgcag taggtccaga cgtccgacat
     2761 caagccatcg ctcgccgggc caac
//
LOCUS       DATMED010000276         5582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4438327, whole genome shotgun sequence.
ACCESSION   DATMED010000276 DATMED010000000
VERSION     DATMED010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4438327"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VNP73_06985"
     CDS             <1..704
                     /locus_tag="VNP73_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65702.1"
                     /translation="VADGAWITLIGPNGAGKTSVLRALAGLVGFGGEIDLGTAPVGAT
                     PRKKLAQLVAYVPQRPFIPPTTTVTDYVLMGRTPYIPYLGTESRTDLDVVAEVLDRLE
                     LNEFGDRELGSLSGGEVQRAVLGRALAQRAPVLLLDEPTSALDVGHQQQVLELVDVLR
                     REASLTVVSTMHDLTLAGQYADDLLLLSNGRSIARGSASEVLREDLIRQHYGASVKVI
                     RDESGLLVVPTRENA"
     gene            701..1267
                     /gene="cobO"
                     /locus_tag="VNP73_06990"
     CDS             701..1267
                     /gene="cobO"
                     /locus_tag="VNP73_06990"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWL65703.1"
                     /translation="MSDQPPVDRPQPPPPTRVPSLVLVNTGHGKGKSTAAFGTMIRAL
                     ARGWKVCVIQFLKSGKWRVGEEEIGKQLGVDWWSIGDGFTWNSTDMDETEAIAREAWR
                     TAKDKISSGEYEMVLLDEITYPMNWDWIATKDVVDTISKRPEHVNVIATGRDAPDELI
                     AIADTVTEMTKIKHAFDKGVRAKRGIDL"
     gene            1268..1780
                     /gene="cobU"
                     /locus_tag="VNP73_06995"
     CDS             1268..1780
                     /gene="cobU"
                     /locus_tag="VNP73_06995"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWL65704.1"
                     /translation="MPLVLLTGGARSGKSRLAVKMATESGLPVTFIATATPTDDDMAA
                     RIARHRTDRPADWTLIEEPLDVARSLSTVEAGACVVVDCLTLWISNLMLEERSQEQIE
                     ELALSTAEVAARRSEPTIVVTNEVGSSLHSTNELGRRFVDTTGRVNQIWGDAADEAYL
                     VVAGGRVRLC"
     gene            1774..2754
                     /gene="cobT"
                     /locus_tag="VNP73_07000"
     CDS             1774..2754
                     /gene="cobT"
                     /locus_tag="VNP73_07000"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWL65705.1"
                     /translation="MLKDLPGPDENSAAAVRERAAQVLRPLGALARLDEVAAWLASWQ
                     RTDRPKVAAPAAAIFVADHGVAERGVSAYPQAVTRGMLDALERGGATAAVLAREQGVA
                     LEVWDVGVGAPTNDFTVEPALSEDRFEQCLDWGRDAVAELDADLLILGEMGIGNTTAA
                     AAVCATLFGLDAEFWTGRGTGVDDATYARKLAVVETASRRVPAGTSPLEILREVGGAE
                     MVAMAGAAVEARRRSIPIVLDGFVVTAALAPLEITQPGALAHCISGHCSSEPGHRLLL
                     EKLDLRPLLDLDLRLGEASGALLALPLVRLAAACVTDVATFQEWGLPGRL"
     gene            2751..3476
                     /gene="cobS"
                     /locus_tag="VNP73_07005"
     CDS             2751..3476
                     /gene="cobS"
                     /locus_tag="VNP73_07005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HWL65706.1"
                     /translation="MNGFLSALTFLTRVPAGARAGDAGSLARGVPWFPVVGVLVGGAS
                     GAVFYFTELFLTPFVAAGLAVVTGVLLTGAFHEDGLADTVDAFGGSHTREDALRIFKD
                     PTHGTFGVLALVATFALRAGALASLSGVAAIAAMCASHALARSSAVSLFAWIEPVREG
                     LGATYASSVSKARVAITVAIGIVVCVAALGTATIAAVVGCALVTLFFGRLTRARIGGL
                     NGDVLGAVEQVCEAVILLVAVAL"
     gene            3473..3997
                     /locus_tag="VNP73_07010"
     CDS             3473..3997
                     /locus_tag="VNP73_07010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWL65707.1"
                     /translation="MKTLLLVRHGATEWSEAGRLCGWSDVPLNAAGRAQATALREQLA
                     EIQPDGVWTSDLKRAAETASLAGLDAHAAADLRELNFGELEGVVWDQLGDDVRSTMIS
                     FHDFAAPGGESVPQLKQRVVRFLDDLDDGHHVLFTHGGVIRLLGSLCGKDRRLLAPCE
                     TVTLEWLPEISSGG"
     gene            4075..4577
                     /gene="rnpB"
                     /locus_tag="VNP73_07015"
     ncRNA           4075..4577
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNP73_07015"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4636..>5582
                     /locus_tag="VNP73_07020"
     CDS             4636..>5582
                     /locus_tag="VNP73_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65708.1"
                     /translation="MREETKTVWRSIAALVFLGTLGAVLARPVFQSGSTEPTSQEVLP
                     SPSPERQRSPKPKKTPPPSPTPTPTPTEEEPSSVELAGSYPKKCLKRAPQTGGSLIAL
                     GWDGQVRVGTTSSIIDTFAGQTPVSWSPSGRYLGFGPGNLYVDGEVDEAALFGGEALQ
                     WTWSPIADCVAGLTADGDLLVGHPGRTRTLIRGATSVLFSPDGRYLKIGLDEAGTEVI
                     NLRKNAIKKGGKKVRFTTGQCADLTDYITPTCSPDGLYVVALQTDGNEGNLGEMWLLT
                     TAGERVREIGEGPYEDTFPEWGPRGTGVLFLRWPDETER"
BASE COUNT          971 a         1717 c         1933 g          961 t
ORIGIN      
        1 atgtcgccga tggcgcatgg ataactctga tcggtcctaa cggagccggc aagacgagcg
       61 tgttgcgagc acttgcgggc ctcgtgggtt tcggtggaga gatcgatctc ggcacggccc
      121 ccgtaggagc gacaccccgc aagaaactgg cgcagctagt cgcctacgtt ccgcagcgtc
      181 ccttcatccc gcccacgacg accgtgaccg attacgtact catggggcgg acgccgtaca
      241 tcccttatct cggaacggag agccggaccg atctggacgt cgtcgccgag gtactcgatc
      301 gtctggaact gaacgagttc ggcgaccgcg agctgggttc gttgagtgga ggagaggtgc
      361 agcgcgccgt tctcggccgg gcgctcgcgc aacgtgcccc ggtcctcttg ctcgacgaac
      421 cgacgagcgc tctcgatgta ggtcaccagc agcaggtgct cgagcttgtc gacgtgctta
      481 ggcgcgaagc gagccttacc gtcgtttcga cgatgcacga cttgacgctg gcgggccagt
      541 acgcagacga cctcttgctc ttgtcgaacg gcagatcgat cgctcgcggt tccgcgagcg
      601 aggtcctccg tgaggacctg atccgacagc actacggtgc gtcggtaaag gtgatccgtg
      661 acgagtccgg ccttctggtc gtgccgacgc gggagaacgc atgagcgacc agccaccggt
      721 ggaccggccg cagcccccgc cacccacgcg cgttccctcg ctcgtcctcg tgaacaccgg
      781 gcacggcaag ggaaagagca ccgccgcatt cggaacgatg atccgtgccc ttgctcgagg
      841 ctggaaggtg tgcgtgatcc agttcctcaa atcgggcaaa tggcgcgtcg gcgaggaaga
      901 gatcggcaag caactcggtg tcgactggtg gtcgatcggc gacggcttca cgtggaactc
      961 gaccgacatg gacgagaccg aggcgatcgc gcgtgaggca tggcgaaccg cgaaggacaa
     1021 gatctcttct ggggagtacg agatggtgct gctggacgag atcacgtacc cgatgaactg
     1081 ggactggatc gccaccaagg atgtcgtgga cacgatcagc aagcgtcccg agcacgtgaa
     1141 cgtcatcgca acgggacgcg acgcaccgga cgagctgata gccatcgcgg acaccgtcac
     1201 ggagatgacg aagatcaagc atgccttcga caagggcgtg cgtgccaagc gagggatcga
     1261 cctctagatg ccactcgtgt tgttgaccgg aggagcgcga tcgggcaagt cgcgactggc
     1321 cgtaaagatg gcgacggagt cggggttacc ggtcaccttc atcgcgaccg ctactcccac
     1381 cgacgacgac atggccgctc ggatcgcgcg gcaccggacg gaccggccgg cggactggac
     1441 gctgatcgaa gaaccactcg acgtagcgag atccctcagc acggttgagg ccggggcgtg
     1501 cgtcgtagtt gactgcctca ccctgtggat cagcaacctc atgctcgaag agcgatcgca
     1561 agagcagatc gaagagctgg cactatcgac ggcagaggtg gcggcacgca ggagcgagcc
     1621 aacgatcgtg gttacgaacg aggtgggctc gagcctgcac tcgacgaacg aacttggacg
     1681 ccggttcgtc gacacgaccg ggcgcgtcaa tcagatatgg ggcgacgcgg ccgacgaggc
     1741 gtacctggtg gtcgcggggg ggcgcgtgcg gctgtgctga aagaccttcc cggcccggac
     1801 gagaactccg ccgcagcagt gcgcgaacgc gcggcccagg tactccgtcc gctgggagcc
     1861 ctcgcacggc tcgacgaggt cgccgcgtgg ttggcgtcgt ggcagcgaac ggatcggccg
     1921 aaggttgccg ccccggcagc cgcgatcttc gtggccgacc atggcgtcgc cgagcggggg
     1981 gtcagcgcgt atccccaggc cgtcaccaga ggaatgctcg acgcgctgga gcgaggcggc
     2041 gccacggccg cggtcctcgc acgcgaacaa ggagtcgcgc tcgaggtgtg ggacgtcgga
     2101 gttggggcgc cgacgaacga cttcacggtc gagcccgcgc tgtcggagga caggttcgag
     2161 cagtgcttgg attggggccg cgacgcggtc gccgagctgg acgccgatct tctgatcctc
     2221 ggcgagatgg gtatcggtaa cacgacagca gcagccgccg tctgcgccac gttgttcggt
     2281 ctggacgcgg aattctggac gggtcgaggt accggggtgg acgacgccac ctacgcgcgg
     2341 aagttagcgg ttgtagagac agcgtcgcgt cgcgtgcccg ccggcacgtc tccgctcgag
     2401 atcctgcgcg aggtcggagg ggctgagatg gttgcgatgg ccggagcggc cgtagaggcg
     2461 cgccgcaggt cgatcccgat cgtcctcgac ggcttcgtcg tgaccgctgc tctggcgcca
     2521 ctggagatca cgcagccggg ggcgttggcg cactgcatct cgggtcattg ctcgtcggag
     2581 ccgggccacc gtctgttgct cgagaaactc gatctccgtc ctttgctcga tctcgacctt
     2641 cgtctgggtg aggcgagcgg tgccctcctg gcacttcctc tggtgaggct tgcggccgcg
     2701 tgcgtgaccg acgtggcgac cttccaggag tggggcctcc cgggacgtct atgaatgggt
     2761 ttctgagcgc actcaccttc ctgacgcgcg ttcctgccgg cgctcgagca ggcgatgccg
     2821 gttctcttgc gcgcggcgtg ccctggttcc cggtcgtcgg agtgttggta ggtggcgcgt
     2881 ccggtgccgt cttctacttc acggagttgt tcctcacacc gttcgtcgcc gcaggcctgg
     2941 ccgtcgtcac aggagttctg cttaccggcg ctttccacga ggacggtctc gccgacaccg
     3001 ttgatgcctt cggaggctct catacgagag aagacgctct ccgcatcttc aaggatccca
     3061 cccatgggac gttcggcgtg ctggcgttgg tcgcgacgtt cgctcttcgc gcgggcgctc
     3121 tggcaagtct gtctggagtc gcagcgatcg ctgcgatgtg tgcgtcgcac gctctcgctc
     3181 ggtcgtctgc ggtgtcgttg ttcgcgtgga tcgagccggt gcgagagggc ctcggcgcga
     3241 cgtacgcgtc atccgtctcg aaggcccgcg tcgcgatcac cgttgcgatc gggatagtgg
     3301 tctgcgttgc agccttgggt acggcgacga tcgccgcggt cgtgggatgc gcgctggtca
     3361 cgttgttctt cggccgtctc acccgggcga ggatcggggg cttgaacgga gatgtcttgg
     3421 gagccgtcga gcaggtgtgc gaggccgtca tcctcttggt tgcggtagct ctgtgaagac
     3481 cctgcttctc gtgcgtcacg gcgcgaccga atggagcgaa gccggtcggt tgtgtggttg
     3541 gagcgacgtc cctctcaacg cggccggccg ggcccaggca accgcgttga gagagcaact
     3601 cgcagagatc cagccggacg gcgtgtggac gtcggacctc aaacgtgcgg ccgagaccgc
     3661 ttcgctggcg ggtctcgatg cgcacgctgc cgccgatctg cgtgagctga actttggcga
     3721 gctcgagggc gtcgtgtggg accagctcgg agacgacgtt cggtcgacga tgatctcgtt
     3781 ccacgacttc gcagctcccg gtggtgagtc ggttccgcag ctgaagcaac gcgtggtccg
     3841 gttcttggac gacctcgacg acggtcatca cgtcctgttc acccacgggg gggtgattcg
     3901 cctgctaggg agcctgtgcg gcaaggaccg ccggctcctg gccccctgcg agacggtcac
     3961 attggagtgg ttgccggaaa tctcatcggg cggataatgg ttgtcaccaa aggccgaacg
     4021 tctcgacgtt cggcccgagg acaggcgatg gtctcgacca tcgccaaaca aatgggagtc
     4081 ggccgggtgg ccgctgtttg caggctcagt ggggcaaatc tcaatgagcc cgaacgccga
     4141 acgtctagac gttcggcggc aaacagagga aagtccgggc tccatagggc aggacgctgg
     4201 ggaaaatccc aggctgggca acccgtggca agtgcaacag agagcagacc gcctctcgat
     4261 ccggatcaag gtccggggga acgccgaacg tctcgacgtt cggcggggag gtaagggtga
     4321 aagggtgagg taagagctca ccagcgcagc cggtgacggt tgcggctagg taaaccccgt
     4381 ccggagcaag gccaagcagg gggttgggtg gcccgcccga cgcccccggg taggccgcat
     4441 gaggccgtcg gtaacggcgg tcccagatgg atggccacct cgcttgtcct ccgcgatcgc
     4501 cgaggcgatc gcggttcggg gtcacgtttg tggcccggac gagcgaacag aacccggctt
     4561 acaggccgac tcgcaactgg gggccctctc cgggaaaccg gagggggccc acccatgtca
     4621 tgcgaatatg cgcccatgcg tgaagagacc aagaccgtat ggcgctcgat cgccgcgctc
     4681 gtgttcttgg gcaccctcgg cgccgtgctt gcccggcccg tcttccaatc gggctcgacc
     4741 gagcccacct cccaagaagt gctgccgtca ccgtcgcccg agcgtcagcg cagccccaag
     4801 ccgaagaaga cccccccacc atcgccaact ccgacgccca caccaaccga ggaggagccg
     4861 agcagcgtcg agctcgccgg cagctatccg aagaaatgct tgaaacgagc tccgcaaacg
     4921 ggagggagcc tcatcgctct cggttgggac ggccaggtgc gggtggggac cacctcgtcc
     4981 atcatcgaca cgttcgcggg tcagacaccg gtttcgtgga gcccctcggg gcgctacctc
     5041 gggttcggcc ccggcaatct ctacgtcgac ggcgaagtgg atgaggcggc cctcttcggt
     5101 ggcgaagcac ttcagtggac ctggtccccg atagcagact gtgtggccgg gctcaccgct
     5161 gatggcgacc tgctcgtagg tcacccaggt cgaacgcgca ccctgatacg aggcgcaacg
     5221 tcggtcctct tctccccgga tggtcgttac ctgaagatcg gtctcgacga ggccgggacc
     5281 gaagtgatca acctacggaa gaacgccatc aaaaagggtg gcaagaaggt gcgcttcacc
     5341 acgggtcagt gtgccgatct caccgactac atcacgccga cttgttcccc ggacgggctt
     5401 tatgtcgtcg cgctacagac ggatgggaac gaggggaacc tgggagagat gtggttgctc
     5461 acgacggccg gcgaacgggt acgcgagatc ggagagggtc cctacgaaga cacgttcccc
     5521 gagtgggggc cgcgcgggac gggggtgctg ttccttcggt ggccggatga gaccgaacgg
     5581 at
//
LOCUS       DATMED010000277         3876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_972237, whole genome shotgun sequence.
ACCESSION   DATMED010000277 DATMED010000000
VERSION     DATMED010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972237"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VNP73_07025"
     CDS             complement(<1..253)
                     /locus_tag="VNP73_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HWL65709.1"
                     /translation="MSSRPVILAYHAIGECPRERDPGNLFVTPAAFERQMSYLARRKK
                     VVSLEQALANPTGDNVIVITFDDAYRNVLTQAGPILARYG"
     gene            complement(250..1362)
                     /locus_tag="VNP73_07030"
     CDS             complement(250..1362)
                     /locus_tag="VNP73_07030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65710.1"
                     /translation="MTLPIIFVSSHARPGGSERYLVTLLDQLGSDHVRDVVVLEEGPL
                     VDKLRSSAQPVTVIPTSARRSSIVLSALRLRRLVKHSRPALVHANGVKAALVTSIALL
                     GTDVPLVWLKHDVSRDGRAARWIASRCARVVGVSEFVMQIFGNLDEDPRYTVVHTGFE
                     PPAVDSTAARRKVLALTGTDEPARIVGMVGRLDRYKGHRDLVEAASLILEQHPDTLFL
                     LVGGDDAAHPDVRPALLELVEHKKVEEAVLFTDHRDDAIELMAGIDIGVIPSRVHPSG
                     WGREGFPMVGLEFLAVETPVVGYDAGGIPELIGDCGLLVPEGNLSALAKAICDLLDDD
                     ARRNELATCGRRRVETQFALESWKDSLRSIYRDVVP"
     gene            complement(1359..2246)
                     /locus_tag="VNP73_07035"
     CDS             complement(1359..2246)
                     /locus_tag="VNP73_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65711.1"
                     /translation="MTSPPTISVVIPTLKRAEELARTLDSVLACDPQPAEIVVVDGDE
                     AHSSRPAVDRAAEATDTPIRHVPSPPGSSRQRNRGLDLVTGNIVLFIDDDVRLTRDAL
                     ARLLDAFAVPTVVGATANVVEIEGRRVGGMHSPIRKLLPGNRRQGSFTSYGYPHRIVD
                     TDVPRDVELMYGCFMAARSEAARRVRFDENLPGYGLAEDEDFAYRLAREGRIVYRPDI
                     DVRHLNLGFRTMDRRRFSERVVVNRSYLFKKNFPQTAASKTRFALLISILFVHRLLNR
                     DVAGARGIIDGIRRRRVTS"
     gene            complement(2243..3706)
                     /locus_tag="VNP73_07040"
     CDS             complement(2243..3706)
                     /locus_tag="VNP73_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HWL65712.1"
                     /translation="MTSDPQVPDATGTRRFAANAAWLIAAEIAGKLASFVFAVIVARG
                     LGPLAYGYFNIAISLGGLVLAFGSLGLNTVVTTEIVRRRDALSEIFSSALVARLGIDL
                     VALVGTIACAPLLVQEPVAVAVVGLVGTALLIDDISSLIGAVFKAVERMRFHALAIFT
                     NRLVSTVLAVVAVAWGGDILAVCVVYLVGSVAGAVFATFSLRRNFSDLKLRAVTRMRM
                     TQLLRAGAPLGVASILNLAVFRLDSLLLQVLKGPIAVAMYGVAFRFFDSLTFLAYSLT
                     SAALPRLARDSRESEPVRAFQLTSSLLLVAYLPIAAGTAFAGRWVVVTIFSARYEAAA
                     ASTTWLAAGVLFYGIAYLGRVALIAADRRREVPRIAAGALVLNVALNLMLIPRLGFEG
                     AAIATFFTEIVDMTLTLVFLRRALPGLRPARVTLVPVVATLVMTIVLMASGVEGPNAI
                     ALGAPVYLVALIGSGYLIARDQSRKALSVLVPRLAAR"
BASE COUNT          813 a         1176 c         1244 g          643 t
ORIGIN      
        1 agccatagcg agcgaggatg ggaccggcct gggtcagaac gttgcgatat gcatcgtcga
       61 acgtgatgac gatcacgttg tcgcccgtgg ggttcgctag agcctgttcg agggacacca
      121 ctttcttacg ccgcgcgagg tagctcatct gccgttcgaa ggccgcggga gttacgaaga
      181 gattgcccgg gtcccgctct cgaggacatt cgccgatcgc gtgataggcc aagatcacag
      241 gtcgcgagct cacggaacca cgtccctgta gatcgaacgc aaggagtcct tccagctctc
      301 cagcgcaaac tgggtctcga cgcgcctgcg gccacatgtc gcaagttcgt tccggcgcgc
      361 atcgtcgtcc aagaggtcac agatggcttt cgctagtgcc gaaaggtttc cttcgggaac
      421 gagtaagcca cagtcaccga tgagctccgg gataccgccc gcgtcgtaac cgacgaccgg
      481 cgtctcaacc gccaagaatt cgagaccaac catcgggaag ccctcgcgac cccagccgct
      541 cgggtggacg cgactgggta tcacgccgat gtcgatgcct gccatgagct cgattgcatc
      601 gtcacggtga tctgtgaata agacggcctc ttccaccttc ttgtgctcaa ccagctcgag
      661 cagggcgggg cggacgtcag ggtgcgcggc atcgtccccg ccgaccagta ggaaaagcgt
      721 gtccgggtgc tgttcgagta tcagtgacgc ggcctcgacc aggtctcggt gccccttgta
      781 tcgatcgagc cggccgacca tccctacgat ccgagccggt tcatcggtac ccgtcagggc
      841 gagcactttt cggcgagccg ccgtcgagtc gaccgcgggc ggctcgaatc ccgtgtgaac
      901 cactgtgtat cgaggatcct cgtccaagtt gccgaagatc tgcatgacga actcgctaac
      961 gccaacgact cgggcgcaac gactcgcgat ccagcgagcg gcccgtccat cgcgactcac
     1021 atcgtgcttg agccagacga gcggtacgtc cgttcccaga agagcgatcg acgtgacgag
     1081 tgcggccttc acgccgttgg catgtacgag ggccggtctg ctgtgtttca ccaacctgcg
     1141 taaacgaagc gcgctcagga cgatagagga acggcgcgca gaggtcggga tgacggtgac
     1201 cggttgtgcg gaggaacgca atttgtcgac cagcggccct tcctccagaa ccacgacgtc
     1261 gcggacgtga tcggatccca actgatctag caatgtgacg aggtagcgct cggagcctcc
     1321 cgggcgagcg tgcgaggaca cgaagatgat cggaagcgtc aacttgtgac ccgccgccgc
     1381 cggatcccgt cgatgatccc tctggccccc gcgacgtccc ggttcaatag tcggtgtacg
     1441 aacaggatcg atatcaacaa cgcgaacctc gtcttcgacg cagccgtttg cgggaagttc
     1501 ttcttgaaga ggtagctcct gttgacgacg actcgctcgc tgaaacgtcg acggtccatc
     1561 gttcggaaac cgagattcag atggcggacg tcgatgtccg gtcggtagac gatccttcct
     1621 tcacgggcca gtcgatatgc gaagtcctcg tcctcggcca gaccgtagcc gggcagattc
     1681 tcgtcgaaac ggacgcgacg ggccgcctcg ctacgagcgg ccatgaagca gccgtacatg
     1741 agttcgacgt ctcgcggtac gtccgtgtcc acgatccggt ggggataccc ataggacgtg
     1801 aacgagcctt gcctgcggtt accgggtagc agcttgcgga tcggagaatg catcccgccc
     1861 acgcgccgtc cctcgatctc gacgacgttc gcggtggctc caaccacggt cgggacggcg
     1921 aaggcgtcga gaagtcgggc caaagcgtct cgggtgagcc tgacgtcatc atcgatgaat
     1981 aagacgatgt tcccggtaac caggtcgagc ccccggttgc gttgccgtga actacccggg
     2041 ggagagggaa cgtgccttat gggcgtgtcg gtcgcttcgg ccgccctatc aacggcgggc
     2101 cgcgacgagt gagcttcgtc tccgtcgacg acgactatct cggcgggctg agggtcgcag
     2161 gcgagcacac tgtcaagagt tcgggcgagc tcttcggctc gcttcagagt ggggatgaca
     2221 acgctgatcg tgggagggga agtcatcgag ccgcaagcct cgggacgagg acggacaacg
     2281 cctttcggga ctggtctcgt gctatcaggt agccgctccc gatcagtgcc accaagtaga
     2341 caggcgctcc gagagcgatc gcattcggcc cctcgacgcc cgaggccatc agcacgatgg
     2401 tcatcacaag cgttgccaca acgggcacga gcgtcacgcg ggcggggcgt agaccgggca
     2461 aggcgcggcg aaggaaaacg agggtcaagg tcatatcgac gatctccgtg aagaaagttg
     2521 cgatggcggc tccctcgaag ccgagacgtg ggatgagcat cagattgagc gccacgttaa
     2581 gcacgagtgc ccctgcagct atgcgtggaa cctcgcgccg tctgtccgca gcgatcaaag
     2641 ccactcgacc gagatatgcg atcccgtaga agagaacgcc cgcggccaac cacgtggtcg
     2701 atgctgctgc cgcttcgtat cgagccgaaa agatggtcac gacgacccac cgtcccgcga
     2761 acgcggtgcc ggcagcgatc ggaaggtaag ccaccagaag caaagacgag gtcaattgga
     2821 aggctcggac gggctcggac tcgcgcgagt cccgagcgag ccgggggagt gcggccgagg
     2881 taaggctgta ggcgaggaac gtcagagaat cgaagaatcg gaaggcgacg ccgtacatcg
     2941 cgacggcgat cggacctttc aacacctgga ggagcaagga atcgaggcgg aagaccgcga
     3001 gattcaagat cgaagcgact ccgagagggg cgccggcccg gaggagttgc gtcatccgca
     3061 tgcgagtgac ggctcttagc ttcaggtcgg agaagttccg cctcaaagag aatgttgcga
     3121 ataccgcgcc ggcgaccgag cccacgagat agacgacaca gacggcgagt atgtctccac
     3181 cccatgcaac cgcgacgacc gccagcaccg tcgacaccag acgattcgtg aagatggcaa
     3241 gcgcgtggaa tcgcatcctt tcgaccgcct tgaaaacggc gccgatcaaa gaggagatgt
     3301 cgtcgatgag tagagccgtt cccaccaacc cgacgacggc tacggccacc ggttcctgca
     3361 caagaagggg agcgcaggcg atcgtcccga ccagggctac cagatcgatt cccagacgag
     3421 cgacgagagc gctcgagaag atctccgaga gagcgtcgcg ccggcgcacg atctcggtcg
     3481 tgaccacggt gttcagtcca agagatccaa aagcaagcac gagcccgcct agcgagatgg
     3541 cgatgttgaa atacccgtaa gcaagggggc cgagcccgcg agcgacgatt acggcgaaga
     3601 cgaagctcgc gagtttgccc gcgatctccg ctgctatgag ccaggcggcg ttggctgcga
     3661 agcgccttgt gccggtcgca tccggcactt gcggatccga tgtcatccgt tactcgtggc
     3721 ggatcggacc gcccgaacga tgtgatcgag atcgacccgg cggaggtcct gatgcacggg
     3781 aagtgcgatc acggtttgcc tcaacctcgc ggagcgcggg aaatcaccgg tcaaactcgg
     3841 gtgcggcacc gcccacaggt cctggacaag catgcg
//
LOCUS       DATMED010000278         3989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2534359, whole genome shotgun sequence.
ACCESSION   DATMED010000278 DATMED010000000
VERSION     DATMED010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2534359"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..2262
                     /gene="recG"
                     /locus_tag="VNP73_07045"
     CDS             34..2262
                     /gene="recG"
                     /locus_tag="VNP73_07045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWL65713.1"
                     /translation="METVAAPPTGLELFGAGSRRRSLPVRGPKKLEALGQLGIRSVQD
                     LLQHYPRRHIDRTKLRTIADLKRLADSGDLGEITLHAEVASISRPFRTTKNPRVKKTL
                     IKGQVKDPTGTLDVTWFNQDWVSRALPVGAQAFFYGRLSDFRGKAQMVAPRFELVKTG
                     AEPFNVGRIVPIYPATAELSSDQLRRVMWDAFGSIDAILDPVPDEIRSRLGLMSRGDA
                     LRLIHFPDAKEDVMQARRRLVFDELFTLQLGLLYRKRKLERTVRGIEHPAPTQDSLPE
                     AFLAALPFEPTVAQRNACDEVASDMNKRYPMHRLLEGEVGSGKTVVALYACLLAIQGG
                     RQAAFMAPTEVLAEQHHLTVDDLLGRAFGSAEATNLFASVSRRPVVRLLTGSTPAAER
                     AKILAETQAGEVDLLIGTHALIQDGVAFAELGVAVVDEQHRFGVHQRKSLREKGTSGE
                     PDILVMTATPIPRTLAVTIYGDLDLSILDELPAGRRPIKTTIAVGDEGREAAYGRISG
                     EVAAGRQAFIVYALRDESNKTELRNAKAEAKRLAKDVFPDLNVGLVHGDMRSQDKEAA
                     MEAMRSGATDILVATTVIEVGVDIPNASVMMIEDADRFGLAQLHQLRGRVGRGGHPSF
                     CILTTDLDLDKAAEDPAVALAAERLHAVEKSGDGFRLAEIDLALRGQGQLFGARQSGM
                     PDLKLADDVFKYEREIKQARDEAAGLLDADPELRAFEHIALEREMRDRFPEQTLDVVQ
                     SG"
     gene            2339..2887
                     /gene="rsmD"
                     /locus_tag="VNP73_07050"
     CDS             2339..2887
                     /gene="rsmD"
                     /locus_tag="VNP73_07050"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWL65714.1"
                     /translation="MRVIAGTAKGRRLKSPPMGTRPMTDQMKESLFNSLGPLDDCYVL
                     DLYAGSGSLGLEAISRGAKKAFFVENARDAIVKLEQNIDATGFKRKTDVVWEDVSRLL
                     SRGADKRMDLIFLDPPYNMPVQSVRADLESIVMGGFLADEGRIVVHRPHKEAQLKPLG
                     LKRFWEKDYGQARLLMFTHEDE"
     gene            2890..3369
                     /gene="coaD"
                     /locus_tag="VNP73_07055"
     CDS             2890..3369
                     /gene="coaD"
                     /locus_tag="VNP73_07055"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWL65715.1"
                     /translation="MTTALCPGSFDPPTNGHIDVIERAARHFEHLVVAVIANPSKQPM
                     FDASERVELLKDSVGHLSNVEVTWFDGLLADFARDRGVHVVVKGLRAVSDFEYELQMA
                     GMNASLYPELDTVFVTANPSWAFLSSSLVKEVSRYGGAIDGLVPPGVAVALKERQSG"
     gene            3472..>3989
                     /locus_tag="VNP73_07060"
     CDS             3472..>3989
                     /locus_tag="VNP73_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division initiation protein"
                     /protein_id="HWL65716.1"
                     /translation="MDLIERIDELQLLVEEAKAVPLSSSAVINRDEFLELLAQLKEAV
                     PDEVRQARWMSRDRDELLARARKEAERIVTEAQEQRDRLLSRTEIVSAAHREAERITD
                     EGKERSARMRIEAEDYIDQKLAAFEILLNKTLSTVARGREQLRGEVQGEGAPQSAADQ
                     SPFDAEEFDQAA"
BASE COUNT          758 a         1224 c         1313 g          694 t
ORIGIN      
        1 gggttccggc ccccgtcctc tagcctgaag aagatggaaa cggtcgcggc tccccccact
       61 ggcctcgagc tgttcggagc gggatctcgt cggaggtccc taccggtgcg aggccccaag
      121 aagctcgagg ctctcggcca gctcgggatc cggagcgtcc aggatctcct tcagcattac
      181 ccccgccgcc acatcgacag gacgaaactg cgcacgatcg cagacctcaa gcgtcttgcg
      241 gactccggcg acctgggcga gataacgctg cacgcggagg tagcaagcat ttcccgcccg
      301 ttcaggacga ccaagaaccc acgcgtgaag aagaccctga tcaagggcca ggtgaaagat
      361 cccaccggca ccctcgacgt gacgtggttc aaccaagact gggtcagccg cgctctacct
      421 gtgggtgcgc aagcgttctt ctacgggagg ttgagtgact tccgggggaa ggcacagatg
      481 gttgccccgc gcttcgagct cgtgaaaacg ggggcggagc cattcaacgt tgggaggatc
      541 gttcccatct atccggcgac cgcggagctg tccagcgatc agctgcgacg cgtcatgtgg
      601 gatgcgttcg gttcgatcga tgccatcctc gacccggtcc ccgacgagat ccggtcgcgt
      661 ctgggactca tgtcgcgcgg tgacgcgctc cggctgatcc atttcccgga cgcgaaggaa
      721 gacgtgatgc aggcccgcag gcggttggtg ttcgacgagc tcttcaccct ccagttggga
      781 cttctctacc ggaagaggaa actcgagcgc accgtgcggg gcatcgagca cccggccccc
      841 acgcaggatt ctcttccgga ggcattcctc gccgctcttc cattcgagcc gacggtcgcg
      901 caaaggaatg cgtgtgacga agtcgcgagc gatatgaaca agcgatatcc gatgcatagg
      961 ttgctggaag gcgaggtcgg gtcgggcaag accgtcgttg cgttgtacgc gtgtctgctc
     1021 gcgatccagg gcggacgaca agccgccttt atggcaccga cggaagtgct tgccgagcaa
     1081 caccacctga cggtcgatga tctgctcggg cgcgcgttcg gttcggccga agccaccaac
     1141 ctgttcgcat ccgtctcgcg ccgtccggtc gtgcgtctgt tgaccggatc gacgccggct
     1201 gccgagcgcg ccaagatact tgcggaaacg caggccggcg aggtcgatct attgatcggc
     1261 actcacgcac tgatccaaga cggcgtcgct ttcgccgagc tgggcgttgc ggtcgtcgac
     1321 gaacagcatc ggttcggggt gcatcagcgc aaatcgctgc gcgagaaggg cacgtccggg
     1381 gagcctgaca tcctagtgat gaccgcgacc ccgatcccgc ggaccctcgc ggtgaccatc
     1441 tacggagatc tcgacctctc gatcctggat gagttgccgg ccggccgtcg tccgatcaag
     1501 acaacgatcg cagtggggga tgagggccgt gaggccgcct atgggcggat cagtggcgag
     1561 gtggccgccg ggagacaggc gttcatcgtc tatgcccttc gggacgagtc caacaagact
     1621 gagcttcgca acgcgaaagc cgaggccaag cgcctggcga aagacgtctt cccggatctg
     1681 aacgtggggc tggttcacgg cgatatgcgc tcgcaggata aagaagccgc catggaagcg
     1741 atgagatccg gcgcaacaga cattctcgtc gccacgaccg tgatcgaggt cggcgtggat
     1801 attcccaacg cgtccgtgat gatgatcgag gacgcggatc gtttcggttt ggcccaactc
     1861 caccaattga gagggcgcgt cggccgcgga ggccacccgt ccttctgcat cttgacgacc
     1921 gatctggatc tggacaaagc agccgaggac cctgcggttg ccctcgcggc cgaacgactc
     1981 cacgccgtgg agaagagcgg cgacggcttc cggctggccg agatcgacct cgccctgagg
     2041 gggcaaggcc agctcttcgg ggcccggcag tccgggatgc ccgacctgaa actcgcagac
     2101 gacgtcttca agtacgagcg ggagatcaag caggcccggg acgaggcggc cgggttgctc
     2161 gacgccgatc ccgagctgag ggccttcgag cacatagccc tagaacggga gatgagggac
     2221 aggttccccg agcagacttt ggacgtggtg cagtctggat agcgcgacgg cctaggccgc
     2281 cgcgcctccg gctagcgacg gtctcgaccg tcgcgtcgga ttgataggct tgcattccat
     2341 gagagttatc gcagggaccg ccaaagggcg gcggctcaag tccccgccca tggggacccg
     2401 gccgatgacc gatcagatga aggaatccct cttcaacagt ctgggccccc tcgacgattg
     2461 ctacgtgctg gatctgtacg caggctccgg ctcgctcggc ctggaggcga tctcgcgcgg
     2521 tgccaagaag gcgttcttcg tggaaaacgc gcgcgacgct atcgtcaagc tcgagcagaa
     2581 catcgacgcg accggcttca agcgcaagac cgatgtcgtg tgggaggacg tgtcgcgtct
     2641 gttgtcgcgc ggcgcggaca agcggatgga cctcatcttc ctggatcctc cgtacaacat
     2701 gcccgtccag tccgttcgtg cagatctgga atcgatcgtg atgggcggct tccttgccga
     2761 cgagggtcgg atcgtcgtac atcgcccgca taaggaggcc cagctgaaac cgctggggct
     2821 caagcgattc tgggagaagg actacggaca ggcgcgctta ctcatgttca cgcacgagga
     2881 cgagtaagca tcaccaccgc actctgtccc ggctcattcg atccacctac caacggtcat
     2941 atcgacgtca tcgaacgcgc tgcgcgccac ttcgagcatc tagtcgtcgc ggtcatagcc
     3001 aatccatcca agcaaccgat gttcgatgcg tccgagcgcg tcgagctgct aaaagactca
     3061 gtcggtcacc tgagcaacgt ggaggtgacg tggttcgacg ggttgctcgc ggatttcgcc
     3121 cgcgaccggg gggtccacgt ggttgtgaag ggattacgcg cggtttcgga tttcgaatac
     3181 gagctgcaga tggccggcat gaacgcctct ctgtaccccg agctggacac cgtgttcgtg
     3241 acggccaatc cgtcgtgggc gttcctgtcc tcgagcctcg tgaaagaggt atcccgctac
     3301 gggggggcta tcgacggctt ggtgccccca ggcgtagccg tagccctgaa ggagcgtcag
     3361 tccggctagg cggtctagga ttgcttggtc cgtttccggg cgggttggtg gcggaaggcc
     3421 ccaggagacg cgaaaatctt cgctaacgca tctgaaagag gtagatcacg catggatctg
     3481 atcgaacgga tagacgagct gcagctcctg gtggaggagg cgaaagcggt tcctctgtcc
     3541 tcgagcgctg taatcaaccg ggacgagttc ctcgagctac tcgcgcaact gaaggaagcc
     3601 gtgcccgatg aggtacgcca ggctcgctgg atgtcgcgcg accgcgacga gctcttggcc
     3661 cgggcccgca aagaggccga gcgcatcgtg actgaggccc aggagcagcg cgaccggctg
     3721 ctgtctcgca ccgagatcgt cagtgcggcc catcgcgagg ctgagcggat caccgacgag
     3781 ggcaaggagc gatcggcccg catgcggatc gaggctgagg actacatcga ccagaagctc
     3841 gcggccttcg agatcctgct gaacaaaacc ctctcgaccg tcgcgcgcgg tcgcgagcag
     3901 ctgcgcggtg aggtccaggg cgagggggca ccgcagtcgg ccgcggacca gagcccgttc
     3961 gatgcggagg agttcgacca ggcggcctt
//
LOCUS       DATMED010000279        11847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4063863, whole genome shotgun sequence.
ACCESSION   DATMED010000279 DATMED010000000
VERSION     DATMED010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4063863"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VNP73_07065"
     CDS             <1..895
                     /locus_tag="VNP73_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWL65717.1"
                     /translation="GFTGYSLLDDLLSGTGLRIAYSIALSIPLIGTWVAFLVFGGEFP
                     AEEIIPRLFVTHVMIIPAMILGLLTIHLAIVWRQKHTDFRGPGRLESNVVGSKLWPTY
                     AVKSVALLFATFAVLFALGGLVQINPVWLYGPFKAQQVTSPAQPDWWMGWVEGALRLF
                     PAWETRAFGFEIPNPFFPAVLLPGITFGLLYAWPFLEARFTGDREPHHLLDRARDHPV
                     RTAMGVATLAFYIVLHLAASNDLLAKALDVPVDRVTTVYRILLFVVPVVGGYVTHRLM
                     KALARSGADRFMEMPLDALKP"
     gene            1035..1325
                     /locus_tag="VNP73_07070"
     CDS             1035..1325
                     /locus_tag="VNP73_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65718.1"
                     /translation="MAQGERDIRREEETAEHFTGGPGVVATKSQAQGAIGGGVLGLIV
                     GALLGLIVGAIVGGAAIWIGPIVFAVGGFVAAGVFGGFFKSQKTREEKTVDV"
     gene            1318..1464
                     /locus_tag="VNP73_07075"
     CDS             1318..1464
                     /locus_tag="VNP73_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65719.1"
                     /translation="MSKERKEGLQNPVIHEGAPSQPEHDTNEKADDRIEIVEPDGTDS
                     PRAD"
     gene            1557..1754
                     /locus_tag="VNP73_07080"
     CDS             1557..1754
                     /locus_tag="VNP73_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65720.1"
                     /translation="MRRIAVLMVGIVIASMTLLASPARAADVCTGGKYNVCVCTENCY
                     CFETVNNAWRAVFGSDIIVCH"
     gene            complement(1937..2008)
                     /locus_tag="VNP73_07085"
     tRNA            complement(1937..2008)
                     /locus_tag="VNP73_07085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1973..1975),aa:Arg,seq:ccg)
     gene            2104..2286
                     /locus_tag="VNP73_07090"
     CDS             2104..2286
                     /locus_tag="VNP73_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65721.1"
                     /translation="MKTKRLLLVTVLSLMSIIPLGSPAQATHNCGFDPCPHPEDVFYV
                     LCMTSIDDKYAPELCA"
     gene            2286..2480
                     /locus_tag="VNP73_07095"
     CDS             2286..2480
                     /locus_tag="VNP73_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65722.1"
                     /translation="MRLARTALVATLACSSLFVVAGPASATCHPERPETCEAEFPPAL
                     EECSTSFDPDLPVKLGVCDL"
     gene            complement(2494..4968)
                     /locus_tag="VNP73_07100"
     CDS             complement(2494..4968)
                     /locus_tag="VNP73_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65723.1"
                     /translation="MRRGTLSVSLIMAGALFVASAPTPGNASTPSGATAAPVQSQPRS
                     QTPEDRYAMAGGCYAIRSAAGGYIVREGDGFTAAGTTLASAEPFYFKATDLGRYLLFA
                     SAEDFLAASEGAVGEAAYGATRSTPGGIVGGLAHEETDKAADQAARSEANNASGRGAS
                     VVAAAAPSALADWKIEEASDGGFVVSLPETEQTLATDGPGQLVLTDGEGTAFALELSD
                     GCASFPEIDINIDGPIPGGANSFQEVRGFLEAHLHAMAYEFIGGRVRCGRPWHRFGVA
                     HALVDCPDHEPGGVGAALEQVLSNSPGPHNTDGWPTFTGWPRPESLTHEQVYYKWLER
                     AWAGGLRLMVNLLVDNGQLCETYPMKRNSCNEMDGVRLQAQRIRELERYIDAQSGGPG
                     EGWFRIVDDPFEARSVINSGKLAVVMGMEVSVPLDCGLSDGQPTCNEDDVVERLGDLY
                     DLGVRQMELVNKFDNAFTGVTGDGGETGLVVNSGNRGETGRFWQLETCGEHDHAHDKE
                     QYNFHDESGSPDELSGRDSIFGGILAVTGASGAAPLYPEGPHCNVLGLSDLGRKVIAE
                     MAKLGMLFDPDHMSARARTQALDFIENDLARVNGLPYSGIVSSHGWADDTIYPRVYEL
                     GGVVTPHAGSSSSFVEKWAKHRAWADPRYYFGLGFGSDMNGFSKQGGARGADAENKVT
                     YPFTGFGGVTVNQQVSGERVYDINTDGVAHYGLYPDWIEDVRLQAGDAFLEDMARGPE
                     AYLQMWERALGIAPDSCRSDYPDLFRADFKELRQGMSPEQVLVALGQPASRAGTNYEY
                     CVEQRWRAAVNFTPDGSLASWKLRRS"
     gene            5057..5434
                     /locus_tag="VNP73_07105"
     CDS             5057..5434
                     /locus_tag="VNP73_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL65724.1"
                     /translation="MAPTKRILICDDDPAILRVLQVNLEVEGYDTLLAHHGEEALEVA
                     VAEHPDLVILDIMMPRMDGYQTCIALKANDSTKDIPVVFLSAKAQDSDIEKGKSYDVV
                     DYLTKPFDPDTLIEVVERVLAEG"
     gene            5431..7083
                     /gene="argS"
                     /locus_tag="VNP73_07110"
     CDS             5431..7083
                     /gene="argS"
                     /locus_tag="VNP73_07110"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWL65725.1"
                     /translation="MITEQLAELVRVAIRSAADEDLIDAVPEPEFERPRKREHGDWAT
                     NVALVAGQGRGNPRKIAEAIVERLPESDLVAKVDVAGPGFLNFHLTPAWLHDVVRSAA
                     DPTSDFGRSVVGSDLKVNVEYISANPTGPMNVVNGRHAAVGDAISNLLEATGHSVTRE
                     YYINDVGRQMTLFARSIEAHYLSHFGRATPVPDDGYQGDYIRALAERIAGDVGDRLVD
                     DPAEERLARLRELGLQHMLASIQASLERFGTSFGVWFSEATLHTSGDVSAAIEKAKAG
                     GYIEEREGAVWLRSSELGDDKDRVVIKANGETTYLASDMPYLADKAKRGFDRMIYLWG
                     ADHHGTVARLMAAAEILGVGRDRVEVLLVQMVNIIEGGEKMRASKRAGVLIPLDELID
                     EVGVDAARYTFLTRSIDQRLDFDIELVKEQAPENPVYYVQYAHARICSILRKGAEEDV
                     SADPSSAKLDVLEHPSEVDLMRKLASFEEEVIEAALRRAPQRIPRFVEALASAFSAFY
                     RDCKVISEDRELTQARLSLCLATKSVLAQGLGLVGVTAPERM"
     gene            7084..7917
                     /locus_tag="VNP73_07115"
     CDS             7084..7917
                     /locus_tag="VNP73_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWL65726.1"
                     /translation="MEFLQLDVFADRAYSGNPLAVFPDGADLSSEQMQAIAREMNLSE
                     TTFVTSVEGDTYSVRIFTPAEELPFAGHPTLGTTWVLRHLGRVMGDDVIQRSAAGETP
                     VSIRGDEVRLRRTGVVTDRQPDLDVIAEMLDLEASALSCTFLGKELTPGKTDAGIEML
                     MVPVSSLDVLGSIFPVTSKVKASGYMGAYCFTPTSETTIRARGFWPMVGVDEDPATGV
                     ACACLGLYLADRVGGFKGEVMQGVEMGRPSRLLLSCDADGVEIGGECRLVLKGTLEAL
                     P"
     gene            7927..9228
                     /gene="lysA"
                     /locus_tag="VNP73_07120"
     CDS             7927..9228
                     /gene="lysA"
                     /locus_tag="VNP73_07120"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWL65727.1"
                     /translation="MHSVLPDTARTNQQGHLEIAGLDAVELADRFGTPVIVFDRSTLE
                     SKARAYTKGLDRDQVSFAGKSFCCVAVCELVAQLGLSLDVCSGGELATALAAGFPPER
                     IMFHGNNKSVAELELAARSGVGRIVIDSFEEIERLAEAGVATKLLIRVTPGVEAHTHE
                     FVQTGQEDSKFGFSLAEGIALDAVKRAMELPGCELTGIHAHIGSNIFELAAFELAVER
                     MADLLAEARDRHGFEASELNLGGGLGIAHTKDELTPDPGEATERLLGFVAREFEARSL
                     SVPRMFVEPGRSLIGPSAVTLYRVGTVKKIPGVRTYVSVDGGMSDNIRPALYGARYEA
                     FLANRMNAPGGPLVTVAGKHCESGDILIKDVHLPENVASGDLLCIPATGAYTYSMASN
                     YNRVTKPPVVMVQDGRATVIVERESNEDLLRYDRRLDGSAL"
     gene            complement(9225..10151)
                     /locus_tag="VNP73_07125"
     CDS             complement(9225..10151)
                     /locus_tag="VNP73_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65728.1"
                     /translation="MKRSIGKAVVMASIICVLSTSLPAQATLPGRNGRLAYGSVRRGN
                     DLEIVVSRSDGSRSKTLTKNNQSDICASWSPDGTRLLFCRFNPKDELFDIWVMNSDGK
                     QKVNLTKTPTEDEIFAAFSPDGEAIAYMHSTTGGSGYDIWLMDPDGSNRRALTTTDED
                     EWRPSWSPDGRTIVFERDVFENGDFVSSDIWTIDINSERETQLTDTDEIEFFADYSPN
                     GNQIAYAADTEGNLDIFVMEDNGNNPRRVTFSPRNETAPAFSPDGSRIAFIAFSKNEE
                     TSNIFVIQSDGADRLRATNTPGREAAPAWQPV"
     gene            10281..>11847
                     /locus_tag="VNP73_07130"
     CDS             10281..>11847
                     /locus_tag="VNP73_07130"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HWL65729.1"
                     /translation="MATKHTVRQRARYRFDTLLAKGTVSLVVWLALATLLLIGVAALI
                     LVVTGIRYSPESQGFMEALWVSLVRTLDPGVVGADAGWAFRAVGLLVTVGGIFIVSTL
                     IGLLAAGINTKLDELRKGRSLVLEKGHTLILGWSPKIMTIITELMIANENQAHSSIVV
                     LAAEDKVFMEDEIRTRVRFNGKTKVVCRTGNPADPLDLHIANPLEAKSVVVLTGAEEA
                     ADAQVIKAVLALLTLDPDFEHLNVVAELTDEQDAAALRRTTRNRISTVVSSDVISRIT
                     AQVCRQSGLSAVYQELLDFGGDEIYFANEPRVAGLSFRDALMSFETSTLIGIRYADGQ
                     IELNPSMDTTLKEDDEVIAISEDDDTVTFTGASQNGDHPIVEGPKSDAEVVERILMIG
                     WSFLGPLILKQLDHYLAPGSHVHVVFDPELSGRAHPEVDTRFDNLTLAVTPGDATDHI
                     FLESLVRDGSFDHVIVVCYRTMPAAESDAKTLMTLVQLRQVMREEGTGAHVSIVTELL
                     EPTDVALAQVANPD"
BASE COUNT         2134 a         3645 c         3736 g         2332 t
ORIGIN      
        1 cgggttcacc ggatactcgt tgctggacga tctcctatcc ggcaccgggt tgcggatcgc
       61 gtattcgatc gctttatcca tccctctgat agggacctgg gtcgcgttcc tcgtgttcgg
      121 aggggagttc cccgccgagg agatcatccc gaggttgttc gtgactcacg tgatgatcat
      181 cccggcgatg atcctgggac tcctgacgat ccatctcgcg atcgtgtggc gtcagaagca
      241 cacggatttc cgggggccgg gcagactcga gtcgaacgtc gtgggttcca agctgtggcc
      301 gacctacgcg gtcaagtcgg tggcgctgct cttcgccacg ttcgcggtgc tgttcgcgct
      361 cggcggactg gttcagatca atcccgtgtg gctgtacggg ccgttcaagg cgcagcaggt
      421 gacctcaccc gcgcagcccg actggtggat ggggtgggtc gagggggctc tgcgtctatt
      481 ccccgcgtgg gagacgcgag cgttcggttt cgagatcccg aatccgttct tccccgccgt
      541 gttgttaccg ggcatcacgt tcgggcttct ctacgcgtgg cccttcctcg aggcgcgatt
      601 caccggcgac agagaacccc accacctgct ggatcgggcc cgcgatcacc ccgtacgaac
      661 ggcgatgggg gtcgcgacgc tcgcgttcta catcgttctg cacctggcag cgagtaacga
      721 tctcctcgcc aaggccctgg atgttccggt ggatcgggtg acgaccgtct accgcatctt
      781 gctgttcgtc gttccggtcg taggtggtta cgtaacgcac agattgatga aggccctggc
      841 ccgcagtgga gccgaccgtt tcatggagat gcccctagac gccctaaagc cctaaccgtt
      901 ctttctaagc ggacttcgac agtcctcgcc ctccgcccac ccatccggcc cccctctagg
      961 gcgggggatt cagaaaagaa cgagacgggt acctacagat cgagcgtcga agatcgcttc
     1021 gaaaggtgga accgatggca caaggcgagc gagacatccg gagggaagaa gaaaccgccg
     1081 aacacttcac cggcggaccc ggcgtcgtag cgacgaagag ccaagctcag ggtgcgatcg
     1141 gcggcggcgt cctcggattg atcgtcggcg cgctcctcgg attgatagtc ggcgccatcg
     1201 tcgggggcgc agcaatctgg atcggcccga tcgtgttcgc ggtcggtgga ttcgtagcag
     1261 cgggtgtctt cggaggtttc ttcaagtcgc agaagacgcg cgaagagaag actgtcgatg
     1321 tctaaagaga gaaaagaggg gctacagaat ccggtaatcc acgaaggcgc accgtctcaa
     1381 cccgagcacg acacgaacga gaaggcggac gatcgcatcg agatagtgga gccggacgga
     1441 accgattccc cccgagccga ctagcccttc ggcgttccgt gccctcggca ggacattccg
     1501 cccgaaacat cgaagcattc atcggctggg atcgaactcg aaggcgggag gaacgcatga
     1561 gaaggattgc cgtgctgatg gtgggaatcg tgatcgcttc aatgacgctg ttggcgtcgc
     1621 cggcccgtgc cgcggatgtc tgtacgggtg ggaaatacaa cgtctgcgtg tgcacggaga
     1681 attgttactg cttcgagacc gtcaacaacg cgtggcgagc ggtcttcggc agcgacatca
     1741 tcgtctgcca ctagcttcca ggatccgtgg gcggctagcc cctcccaggg cccttgttgc
     1801 gtcaccttcc gtgccccctg ccggttcgaa cccttacctt tcgcggatca cttacgtgaa
     1861 attgtggcgt ggtctcgaac cgcccgtgtc actccggccc ttccggcgct cgcaagcggc
     1921 gcctccacgc acgtcgtgcc cccggcagga ttcgaacctg cgcacctcgc tccggaggcg
     1981 agtgctctat cccctgagct acgggggcgc gccccaactc tagacggcag ggaggattcg
     2041 gcccctcccg tgacgaagtc accccacagc aacccggggt gccgccttcg aaaggggtcg
     2101 atggtgaaga cgaagagact gctgctggtg acggttctgt cactgatgag catcatcccg
     2161 ttgggttctc cggctcaggc gacgcacaac tgcggcttcg atccgtgccc gcatcccgaa
     2221 gatgtcttct acgtcctttg catgacctcc atagacgaca agtacgcgcc ggagctctgt
     2281 gcttgatgag gcttgcacgc accgctttgg tcgcaaccct tgcttgctcg agcctgttcg
     2341 tcgttgccgg accggcatcc gcaacgtgcc atcccgagcg acccgagaca tgcgaggcgg
     2401 agttcccgcc cgccctcgag gaatgctcga cgagtttcga cccggatctg ccggtgaaac
     2461 tgggagtctg cgacctctaa gggatacgac gagttagctg cggcgcagct tccaggaggc
     2521 caggcttccg tccggggtga agttgaccgc cgctctccac cgctgctcga cgcagtactc
     2581 gtagttcgtt cccgctcggg aggccggctg gccgagtgcc acgaggacct gctcggggct
     2641 catcccttgc cgtagctcct tgaagtcggc cctgaacagg tcgggatagt cgctgcgaca
     2701 ggaatcgggg gcgatgccca gcgcgcgctc ccacatctgc aggtacgcct ccgggcctcg
     2761 ggccatgtcc tcgaggaacg cgtcgccggc ttggagccga acgtcttcga tccagtcggg
     2821 gtacaggccg tagtgcgcta ctccatcggt gttgatgtca tagacgcgct ctccgctcac
     2881 ttgttggttg accgtgacgc cgccgaaacc ggtgaaggga taggtcacct tgttctcggc
     2941 atccgctccc cgggccccac cctgtttgct gaacccgttc atgtccgagc cgaatccgag
     3001 accgaagtaa taacgggggt cggcccaagc cctgtgcttg gcccacttct ccacgaagct
     3061 gctcgaacta ccggcatgtg gggttacgac accgccgagc tcgtagacac gcggatagat
     3121 cgtgtcgtcg gcccatccgt ggctggacac gatgccggag tagggaagac cgttcaccct
     3181 ggcgagatcg ttctcgatga agtcgagcgc ttgcgtccga gcacgggcgc tcatgtgatc
     3241 ggggtcgaag agcatcccca gtttcgccat ctcggcgatc accttgcggc cgagatcact
     3301 cagtccgaga acgttgcagt gcgggccctc cgggtacaac ggcgccgcgc cggacgctcc
     3361 ggtcacagca aggatgccgc cgaagatcga atcccggccc gagagctcgt cgggggagcc
     3421 cgactcgtcg tggaagttgt actgctcctt gtcgtgcgca tgatcgtgct caccgcaggt
     3481 ctcgagctgc cagaaacgac cggtctctcc ccggttgcct gagttcacga cgagtccggt
     3541 ttcacctccg tcgccggtaa cccccgtgaa tgcgttgtcg aatttgttga cgagctccat
     3601 ctggcgaaca ccgaggtcgt agaggtcacc gagccgttcc acgacatcgt cttcgttgca
     3661 cgttggctga ccgtcgctga ggccacaatc cagcggcacc gatacctcca tgcccatcac
     3721 aaccgcgagc ttgccggagt tgatgaccga gcgagcttcg aagggatcgt cgacgatgcg
     3781 gaaccaacct tcgccgggtc ccccgctctg cgcatcgatg tagcgctcca gctcgcggat
     3841 gcgttgagcc tgcagccgga caccatccat ctcgttgcag ctgttgcgct tcatcgggta
     3901 ggtctcgcac agctggccgt tgtcgaccaa gaggttgacc atcaatctca gtccgcccgc
     3961 ccacgcacgc tcgagccact tgtaatagac ctgctcgtgg gtgagcgact ccggccgcgg
     4021 ccaaccggtg aaggtcggcc agccgtcggt gttgtggggg ccggggctgt tgctcagcac
     4081 ctgctcgaga gccgcgccga cacctcccgg ctcgtgatcc ggacagtcga cgagtgcgtg
     4141 agcgaccccg aaccggtgcc acggtcgccc acagcgaacg cgcccgccga tgaactcgta
     4201 cgccatcgcg tgcaggtggg cctcgaggaa gccgcgtacc tcctggaagc tgttggcgcc
     4261 gccggggatc gggccgtcga tgttgatatc gatctcgggg aacgaggcgc agccgtcgga
     4321 caactcgagc gcgaaggcag ttccttcacc gtcggtcagt acgagttgtc ccgggccatc
     4381 ggtcgccaga gtctgttcgg tctcagggag tgagacaacg aatccgccgt cactcgcctc
     4441 ttcgatcttc cagtcggcga gagcgctcgg agccgccgcg gccacgaccg atgccccgcg
     4501 tccgcttgcg ttgttagctt cgcttcttgc cgcttggtcg gcggccttgt cggtctcttc
     4561 gtgcgcgagt ccacccacga tcccacccgg cgtcgagcgg gtggctccat aggcggcttc
     4621 gcccacggcc ccttcgcttg ccgcgaggaa gtcctcggcg gacgcgaaca gaaggtagcg
     4681 accgaggtcg gtggccttga agtagaacgg ttcggcactc gcgagcgtcg tccctgccgc
     4741 cgtgaatccg tcgccctctc ggacgatgta tccgccggca gcggagcgga tcgcgtagca
     4801 gccgccggcc atcgcatagc gatcttcggg cgtctgcgag cggggctgcg attggaccgg
     4861 ggccgcggtt gcgcccgagg gcgtgctcgc gttgccgggg gtgggggccg acgccacgaa
     4921 caacgcgccg gccatgatca aagacacgct gagggtgcct cgacgcatgg ggagtccttt
     4981 ccttggtttg gtgttgaagt tctccgcggg gagggcgctt ccctgctagg ggcgaaaggg
     5041 tctgtagcat ccgcccatgg cccccacgaa gcggatcctg atctgcgacg acgacccggc
     5101 catcctgagg gtcctccagg tcaacctcga ggtcgagggt tacgacacgc tccttgccca
     5161 ccacggggag gaggcgctgg aggtcgccgt cgccgagcac ccggatctcg tgatcctgga
     5221 catcatgatg ccgcgtatgg acggctatca gacgtgcatt gccctcaagg ccaacgattc
     5281 cacgaaggac atccccgtcg tcttcttgtc ggcgaaggcc caggattccg acatcgagaa
     5341 gggcaagtcc tacgacgtcg tggactacct cacgaagccg ttcgatcccg acaccttgat
     5401 agaggtcgtc gagcgggttc tcgcagaagg gtgataaccg agcagcttgc cgaactcgtc
     5461 cgtgttgcca tccggtcggc ggcggatgag gacctgatcg atgccgttcc cgaacctgag
     5521 ttcgaacggc cccggaagcg cgagcacggc gactgggcga ccaacgttgc tctcgtagcc
     5581 ggccagggtc gaggtaaccc ccgcaagatc gccgaagcga tcgtcgaacg ccttccggag
     5641 tccgatctgg tggcgaaggt cgacgtggcc gggcccggct tcctcaactt ccacctcact
     5701 ccggcgtggc ttcacgacgt cgtgcgcagc gcggcagatc ccacttcaga tttcggtcgc
     5761 agcgtggttg gttcggactt gaaggtcaac gtcgaataca tctccgctaa cccgaccggt
     5821 ccgatgaacg tcgtgaatgg acgtcacgcc gcggtcggcg atgccatctc gaatctcctc
     5881 gaggcgaccg gacacagcgt tacgcgcgag tactacatca acgacgtcgg tcgccagatg
     5941 accctgttcg cacgctctat cgaggctcat tacctatctc acttcggtcg agccactccc
     6001 gtgcccgacg atgggtatca gggcgactac atccgcgctc tagcggagcg gatcgcgggc
     6061 gacgttgggg atcgactcgt cgacgacccg gccgaggagc ggctcgctcg tctccgagaa
     6121 ttgggactgc agcacatgct cgcatcgata caagcgtcgc tcgagaggtt cggaacgagc
     6181 ttcggcgtgt ggttctcgga ggcgaccctc cacacgagcg gtgatgtgag cgcggcgatc
     6241 gagaaggcga aagccggcgg ctatatcgag gagagagaag gagcggtgtg gctacgatcg
     6301 tccgagctgg gtgacgacaa ggaccgcgta gttatcaagg cgaacggcga gacgacctac
     6361 ctcgcttcgg acatgcccta tctggcggac aaggcgaagc gtggcttcga ccggatgatc
     6421 tacctgtggg gagccgacca tcacggcacc gtcgcgcgct tgatggccgc ggccgagatc
     6481 cttggagtgg gtcgagaccg ggttgaagtc ctacttgttc agatggtcaa catcatcgaa
     6541 ggcggcgaga agatgcgtgc gtccaagcgc gccggcgtgt tgatacccct agacgaactc
     6601 atagatgaag tcggtgtgga tgctgcgcgc tacacgttct tgacgagatc gatcgaccag
     6661 cgtttggact tcgatatcga gctcgtgaag gagcaggccc ccgagaaccc cgtctactac
     6721 gtgcagtacg cgcatgcgcg tatctgctcg atcctgcgca agggagccga ggaggatgta
     6781 agcgccgacc cgagctcggc gaaactcgac gttctcgaac atccgagcga agtcgacctc
     6841 atgcgcaagc ttgcttcatt cgaagaggaa gtcatcgagg ctgcactccg cagggctccg
     6901 caacggatcc cgcgattcgt cgaggctctc gcctcggcgt tcagcgcctt ctatcgcgat
     6961 tgcaaggtca tctcggagga ccgggaactg acgcaggcac gtttgtcgct gtgtcttgcg
     7021 acgaagagcg ttctcgccca aggattgggc ctagtcggcg tgaccgcgcc ggaaaggatg
     7081 tagatggagt tcctacagct cgacgtcttc gccgaccgtg cctacagcgg gaacccgctc
     7141 gccgtgtttc ccgacggagc ggatctgtcc tccgagcaga tgcaggcgat agcgcgtgag
     7201 atgaacctct cggagaccac gttcgtcacg tcagttgagg gggacaccta ctcggttcgg
     7261 atcttcacgc cggctgagga acttccgttc gcaggccatc ccacactggg cacgacatgg
     7321 gtgttgcgcc atctggggcg cgttatgggc gacgacgtga tccaacgcag cgcggccgga
     7381 gagactcccg tctcgattcg cggtgacgag gtgaggctcc gtcgtacggg tgtcgtcacc
     7441 gaccgacaac ccgacctcga tgtgatcgca gagatgctcg atctggaggc ctcggcgctg
     7501 tcctgcactt tcctggggaa ggagttgact ccgggcaaga ccgatgccgg catcgagatg
     7561 ttgatggttc cggtgtcgag cctcgatgtg ctcggctcga tctttccagt gacctccaag
     7621 gtgaaagcct ccggctacat gggcgcctac tgcttcactc cgacgtccga aaccaccatc
     7681 agggcgcgcg ggttctggcc gatggtgggc gtcgacgagg acccggcgac cggagtcgcg
     7741 tgcgcatgtc ttgggctcta tctcgctgat cgtgtgggtg gattcaaggg cgaggtgatg
     7801 cagggggtcg agatgggtcg accttctcgc cttctgctta gttgcgacgc cgacggggtc
     7861 gagatcggcg gcgaatgccg gttggtgctg aaaggaacac tcgaagcgct gccgtaacct
     7921 ctatagatgc attccgtgct tccggacacc gcgcgcacta accagcaagg ccatctcgag
     7981 atagccggtc tcgacgcggt cgagttggcc gatcgcttcg gaacccccgt gatcgttttc
     8041 gatcgatcga ccctggagtc aaaggcccgt gcctatacca aaggcctgga ccgagatcag
     8101 gtgtctttcg cgggaaagtc cttctgctgc gttgctgtgt gcgagctcgt ggctcagctt
     8161 gggctcagcc tggatgtctg cagcggcggc gaattggcaa ccgctttggc cgccggattt
     8221 cccccagaac ggatcatgtt ccacggcaac aacaagtccg tcgcggaact cgagctagcg
     8281 gcgcgatcgg gcgtcgggcg tatcgtcatc gattcattcg aagagatcga acgcttagcc
     8341 gaggccggtg ttgcaaccaa acttttgata cgggtaactc cgggggtgga agcacatacc
     8401 catgaattcg ttcagaccgg acaagaggac tcgaagtttg gcttcagctt ggctgagggc
     8461 atagcactcg atgcggtgaa gcgtgcgatg gaacttcccg gctgcgaact gaccggtatc
     8521 catgcacaca ttggatccaa catattcgag cttgccgctt tcgaactggc ggtcgagcgc
     8581 atggccgatc ttcttgccga ggcacgggac cgacacggct tcgaagcttc agagctgaac
     8641 cttggtgggg gattaggcat cgcgcacacg aaagacgagt tgacacccga ccccggtgaa
     8701 gcgaccgagc gactgctcgg gttcgttgcc cgagagttcg aagccagaag tctgtcggtt
     8761 ccgcggatgt tcgtggaacc cgggcgctct ctcatcgggc ccagcgccgt aaccctgtac
     8821 cgggtcggaa ccgtcaagaa gatcccgggc gtgcgtacct atgtttcggt ggacgggggg
     8881 atgtcggaca acatccgccc tgccttgtac ggcgccagat acgaggcctt ccttgccaat
     8941 cggatgaacg cgcccggcgg cccattagtg acggtggccg ggaaacactg cgagtcgggc
     9001 gacatcctga tcaaggatgt ccatctccct gagaacgtgg cgtccggcga cctgctgtgc
     9061 atccctgcga cgggcgccta cacctattcg atggcttcca actacaaccg ggtcacgaaa
     9121 ccgccggtcg tgatggtgca agacggccgg gcgacggtga tcgtcgaacg cgagtccaac
     9181 gaggatcttc ttcgttacga ccgccgcttg gacggctcgg cgctctagac cggttgccag
     9241 gcgggcgcag cttcgcgtcc cggagtgttc gtggcgcgta ggcggtcggc gccatcggac
     9301 tggatcacga agatgttgga ggtttcttcg ttcttcgaga acgcgatgaa ggcgatcctg
     9361 cttccgtccg gcgagaaggc cggggcggtc tcgttgcggg ggctgaatgt cacccgacgg
     9421 ggattgttgc cgttgtcctc catcacgaag atgtcgagat tcccttcggt atcggccgcg
     9481 tacgcgatct gattcccgtt cgggctgtag tcggcgaaga attcgatctc gtcggtgtcc
     9541 gttagctggg tctcccgttc cgagttgatg tcgatcgtcc agatatccga agacacaaag
     9601 tcaccgttct cgaacacgtc gcgttcgaac acgatggttc tcccatccgg ggaccaactc
     9661 ggacgccatt cgtcctcgtc ggtggtggtg agcgcgcgac ggttactacc gtccgggtcc
     9721 atcagccaga tgtcgtagcc actccctccg gtggtgctgt gcatgtaggc gatcgcttct
     9781 ccatcggggg agaaggcggc gaagatctcg tcttcggtgg gggtcttggt gagattcacc
     9841 ttctgcttac cgtccgagtt catgacccag atgtcgaata gctcatcttt cggattgaaa
     9901 cggcagaaca acagccgcgt gccgtcgggg gaccacgacg cgcagatatc cgattggttg
     9961 ttcttcgtca gtgtcttcga ccgggatccg tcactgcgcg atacgacgat ctcgagatcg
    10021 ttgcctctgc gcacggaacc gtaggcgagg cggccgtttc ttccggggag ggttgcctgt
    10081 gcgggaagcg atgtggacag aacgcagatg atcgaagcca tcaccacggc tttgccgatc
    10141 gaccgtttca cctgggacct ccgccttgat gtcgacgcca cctaatcatc tttatcggcg
    10201 cattgcatca ctcttctgag ggcggattcg ttcgcttttc cccttgcggg acctccggac
    10261 gattggtcag gcttagaggg atggctacca aacacaccgt ccggcagcga gctcgctatc
    10321 gcttcgacac cctcctggcg aaggggaccg tctccctcgt cgtgtggctg gccttagcca
    10381 ctctgctgtt gatcggcgtt gccgcactca tcctcgtagt caccggcatc cgttatagcc
    10441 ccgagtcgca gggcttcatg gaagcgttgt gggtgagcct cgttcggacg ctcgatcccg
    10501 gcgtggtggg cgcggatgcc gggtgggctt tccgggccgt cgggttgctc gtcaccgtgg
    10561 gcgggatctt catcgtcagt acgctcatcg gtctcctggc cgcgggtatc aacaccaagc
    10621 ttgacgaact gaggaagggc cgctcactcg tacttgagaa gggtcacacc ctgatcctgg
    10681 gttggtctcc caagatcatg acgatcatca ccgagctgat gatcgcgaac gagaaccagg
    10741 cccactcgtc gatcgtcgtg ttggcggcgg aggacaaggt cttcatggaa gacgagatca
    10801 gaactcgtgt gaggttcaac gggaagacga aggtcgtgtg ccgtaccgga aaccctgccg
    10861 atcctctcga tctgcacatc gccaatcccc tcgaagcgaa gtcggtagtg gttctgaccg
    10921 gagccgaaga ggctgcagac gctcaagtga tcaaggcagt ccttgcgctg ttgacgctcg
    10981 atcccgactt cgagcatctg aacgttgtcg cggagttgac cgacgagcag gacgctgccg
    11041 cattaaggcg tactacacgg aaccggatct ccaccgtcgt gtcgagcgat gtcatctcga
    11101 ggatcacggc acaggtctgt aggcagtcgg gtctcagcgc cgtctaccaa gagctcttgg
    11161 atttcggtgg tgatgagatc tatttcgcca acgagccccg agtagcgggg ctgtcctttc
    11221 gtgacgcgct gatgtccttc gagacatcga ccttgatcgg gatccgctac gcggatgggc
    11281 agatcgaact caacccctcg atggacacga ctctcaaaga ggacgatgaa gtgatcgcga
    11341 tctcagagga tgacgacacc gtgaccttta cgggtgcgtc gcagaacggt gatcacccga
    11401 tcgtcgaggg acccaagagc gatgccgagg tggtcgaacg catcctgatg atcggatgga
    11461 gcttcctcgg ccccctcatc ctcaagcagc tcgatcacta cctcgcgccg ggcagtcacg
    11521 ttcatgtcgt cttcgatccc gagctctcgg gccgagccca ccctgaggtc gatactcgct
    11581 tcgacaacct gaccctcgcc gtcactccgg gggacgcaac cgaccacatc ttcctcgagt
    11641 ccctggtgcg ggacggttcc ttcgaccacg taatcgtcgt ctgttaccga acgatgccgg
    11701 ctgcagaatc ggacgcaaag acgctcatga ccctcgtgca gctgcgacag gtgatgcgcg
    11761 aggagggaac cggtgcccac gtttcgatcg tcacggagct gctcgaaccc accgacgttg
    11821 cgctcgcgca ggtggccaac cccgatg
//
LOCUS       DATMED010000280         6195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1372169, whole genome shotgun sequence.
ACCESSION   DATMED010000280 DATMED010000000
VERSION     DATMED010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372169"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1083)
                     /locus_tag="VNP73_07135"
     CDS             complement(199..1083)
                     /locus_tag="VNP73_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWL65730.1"
                     /translation="MTPRWKTSLAISVLTALVLAACGGGGTTTDPGAGGDPAEEQPAF
                     EVLNEGRLTVGSDIPYPPFEFEKDGVLTGYDVEIVREIASRLGLENTDDDWISTDFGT
                     IFQQLKSGTKFDIVVAAVTGYAAPGSAAEETVAERTKLIDFTAPYYPSLQSLMVPKGS
                     DIKSTDDLTDGVRVAVQNATTGAFHAEKEYGAQGAEIVGFEKAPTMFQSLQGGNVDAI
                     VNDLPVSLDAMKQFPDLEVIEQIPTGEEYAIAVSKDNAELKDAVNDALAEMFEDGTFA
                     TIYKKYFPEQELPEYASE"
     gene            complement(1086..1811)
                     /locus_tag="VNP73_07140"
     CDS             complement(1086..1811)
                     /locus_tag="VNP73_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65731.1"
                     /translation="MAGKTLLELNDVRVRYGVIEAVKGINLTVHDGEIVALIGGNGAG
                     KTTTLRAISMVHGPSQGKIVFDGEDLTSLRSHDVVDRGVIQVPEGRHIFPNMSVQENL
                     VMGSFRVKGAKSEFQQILDRVFELFPRLQERKKQAGGTLSGGEQQMLAIGRALMSKPK
                     LLMLDEPSMGLAPQLVETVFAVIEEINSQGIPVLLIEQNAAMALQTANRGYVIETGEI
                     VLEDEAKKLLGNDQVRKAYLGEE"
     gene            complement(1801..2619)
                     /locus_tag="VNP73_07145"
     CDS             complement(1801..2619)
                     /locus_tag="VNP73_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65732.1"
                     /translation="MAAFLTVKDVTMEFGGLKALSRVNFEINKGEIFGLIGPNGAGKT
                     TMFNCVTGVYTPTGGQVSFDGQSITGKRPDQIVDVGICRTFQQIRLFPNMTALENVFI
                     GVDARHQTSVFGALFRSGRHRREETEGEEQALNLLEYCGLRKYANDLAKNLSYGDQRR
                     LEIARAMGTKPSLLLLDEPAAGMNPVEKNGLRDLVRKIRDEGITVLLIEHDMKVVMGL
                     SDRVAVLDHGELIAYGQPEDVQKDPKVIEAYLGSGAGGQAAVGEDDRGGDQVGR"
     gene            complement(2619..3785)
                     /locus_tag="VNP73_07150"
     CDS             complement(2619..3785)
                     /locus_tag="VNP73_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65733.1"
                     /translation="MAVVQEQFDRVRGSYRDWEAGLGGPARIGLKIARYALAAFLMAM
                     IPVLMERGYPPFSWVRLEGFFGTEVLFNTGLWILLALGLSVVVGLAGLLDLGYVAFFM
                     LGAYTVALMTDSGNVGQFHLVEWPTWAALPFCILVAMFAGFTLGVPTLRLRGDYLAIV
                     TLGFHEIVRQTANNLTITNGSRGIPGIPSPVIDLPFLHLEFGLETRYYWWFLLIPAIL
                     LAVLMIRHLDNSRVGRYWAAIREDEVAAAAMGVPVTKMKVLAFVIGASTSGVAGWIYA
                     TKISFIAPNSFPLLQSILVLCAVVIGGLGSISGAVLGAAIVIALPEIIREFSGAEPVL
                     GFDVLTGRIAIFGLLLIVVMIYRPGGLLAARRRRTELQEAGAAGAVIHEEVVES"
     gene            complement(3785..4729)
                     /locus_tag="VNP73_07155"
     CDS             complement(3785..4729)
                     /locus_tag="VNP73_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL65734.1"
                     /translation="MDCNFGLVGTQLVTGIILGLLYALVALGYTMVYGVLKLINFAHD
                     AVFALGGIAVFFVIGEWLGLNGPFSGIQLMFMVLFVLLVGGLVSAGGAVLLERVAYRP
                     LRKRNAARLSYLISAIGASFFATYLFQQNFMLGPAKRGFPTLFASGEEGFSVQILGTR
                     VIPTGILLVVVAILCFVILDRVINKTNTGRSIRALSEDPVAAQLMGINVDRVIVTTFL
                     IGGFFGGVAGALYGLRFDNVVWNMGFIMGIKAFTSAVLGGIGNIRGAFLGAVLLGLLE
                     NMSTICISAEWKNVLAFALLPLVLIFRPTGIMGERIGG"
     gene            complement(4838..6034)
                     /locus_tag="VNP73_07160"
     CDS             complement(4838..6034)
                     /locus_tag="VNP73_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWL65735.1"
                     /translation="MSRRLHLVAAAAAMALVAAACGGGGDGGGGPAAEGPGDCTWTIA
                     TMGALSGDYASVGQPIADGVEYAVNAANEEGNLACQLEFVSEDSQGDPNPAPGLAQKI
                     VSNQSVVFCACPYFSGETLATGKIFGKAGVAISGTGTNETIDEQGFETWFRAVAPDNI
                     QAEVAGAYIRDGLKAKAVAVVHDNQDYSKGLAEGVAKALGDLVTDTYVINPEETDYSA
                     VVGQVADASPDVVFYGGYTPQAGPLAKQLVDAGVDATFMTDDGSKDPAFGDTAKDAAE
                     GALASCPCADPLKVDGAEDYVSAMQEAYGKNAPGTFAADMTDVTNLAIEALAAYEGDA
                     EDITAVRKAVVDYFAAADGIQGITKAYAWNDDGEFEGGPGDVWIYEWDMKAKNFLSLG
                     PASELL"
BASE COUNT         1197 a         2023 c         1818 g         1157 t
ORIGIN      
        1 acgacgcttc ccagcagcaa cagcgccatc gccgccgctc ccgtcgccag ggtggcgagc
       61 ccttggggag tgctctgctc tcgcacccat gaccaacccc tctcggggtg gacggccgtt
      121 cgtgaagcca atggttcctc ctgaatcaat gaggcccgcg catcgctgcg cgggcctcaa
      181 gtatctgctg cctggctcct actcggaggc gtactctgga agttcttgtt cggggaagta
      241 cttcttgtag atcgtcgcga acgtgccgtc ctcgaacatc tcggctaggg catcgttcac
      301 cgcgtccttc agctccgcgt tgtccttcga aacggcgatc gcgtactcct cacccgtggg
      361 gatctgctca atgacttcga ggtcggggaa ctgtttcatt gcatcgagcg agaccggaag
      421 gtcgttgacg atcgcatcta cgtttccacc ctgaagcgac tggaacatcg tcggtgcctt
      481 ctcgaatccc acgatctcgg ctccttgagc cccgtactcc ttctcggcgt ggaaagcacc
      541 ggtcgtcgcg ttctgaaccg cgacgcgtac cccgtcggtc aagtcatccg tgctcttgat
      601 gtcgctcccc tttgggacca tgagcgattg caatgacggg tagtaggggg cggtgaaatc
      661 gatcaacttt gtgcgctccg ctacggtttc ctcggcggca gacccggggg ctgcataacc
      721 ggtcacagcc gcgacgacga tgtcgaactt ggttccggac ttcaactgct ggaagatcgt
      781 tccgaagtcc gttgagatcc agtcgtcgtc ggtgttctca agaccgaggc gagaggcgat
      841 ctctctgacg atctcgacgt cgtaccccgt gagcacgccg tctttctcga actcgaacgg
      901 cggatagggg atgtcggaac ccaccgtcaa acgaccttcg ttcagaacct cgaacgccgg
      961 ctgctcctcc gcgggatcgc cacccgcacc ggggtcagtt gttgtgccgc caccaccgca
     1021 cgccgccagg acgagcgcgg tcaatacgga aatagccagc gatgtcttcc accgtggagt
     1081 catcgtcact cctctcccag gtatgccttg cgtacctgat cgttgcccaa aagtttcttc
     1141 gcctcgtctt caagcacgat ctcgcccgtc tcgatcacgt agccacggtt ggcggtctgc
     1201 aacgccatcg cggcgttctg ctcgatcagc agaacgggga tcccttgcga gttgatctcc
     1261 tcgatcaccg cgaagaccgt ctcgacgagc tgcggagcga gccccattga gggttcatcc
     1321 aacatcaaca acttcggctt gctcatcagg gctctgccga tcgcgagcat ctgctgctca
     1381 cctcccgaca gggttccccc ggcctgcttc ttgcgttctt ggagacgggg gaagagctcg
     1441 aagacccgat caaggatctg ctggaactcg gacttcgcac ccttcactcg gaacgagccc
     1501 atgacgaggt tctcttgaac ggacatgttg gggaagatgt gtcggccctc tggtacctgg
     1561 atgacgcctc gatcgacgac gtcgtggctc ctaagcgagg tcaggtcctc accgtcaaac
     1621 acgatcttgc cctgagacgg accgtggacc atggagatgg cacgcagtgt cgtcgtcttg
     1681 ccggcgccgt tcccgccgat caaggcaacg atctcaccat cgtggaccgt gaggttgatc
     1741 cctttgacgg cttcgataac gccgtagcgc actcggacgt cgttgagctc gaggagcgtc
     1801 ttacctgcca acttgatctc ccccgcgatc atcttcgccc actgcagctt gaccgccggc
     1861 gccggagcct agataggcct cgatcacctt tggatccttc tgcacatctt cgggctgccc
     1921 gtaagcgatc aactcgccat gatcgaggac cgcaacgcga tccgacaagc ccataaccac
     1981 tttcatgtcg tgctcgatca acagaacagt gatcccttcg tcgcggatct tccggacgag
     2041 gtcgcgcaga ccgttctttt cgaccgggtt catcccggcc gcgggctcgt ccaaaagaag
     2101 gagcgatggt ttggtgccca tggcgcgcgc gatctccaac ctccgctggt cgccgtacga
     2161 gaggttcttc gccaggtcgt tcgcgtactt tcgcaggccg cagtactcga gcaggttgag
     2221 tgcttgctct tcgccctcgg tctcctcccg gcgatgccgg ccgcttcgaa acagtgctcc
     2281 gaagacgctc gtctgatgac gggcatccac gccgatgaac acgttctcta acgcagtcat
     2341 gttcgggaac aaccggatct gttggaaggt ccggcagatg cccacgtcga cgatctggtc
     2401 gggtcgcttt cctgtgatgg attgaccgtc gaagctgacc tggcccccgg taggcgtata
     2461 gacgcccgtc acgcagttga acatcgtggt cttgcccgcg ccgttagggc cgatcagccc
     2521 gaagatctcg cccttgttga tctcgaagtt gacccgggac agtgccttga gaccaccgaa
     2581 ctccatcgtg acgtctttga cggtgaggaa agcagccatc agctctcgac cacctcttcg
     2641 tggatgacgg cgccggccgc gcctgcttct tgcagctcag tgcgtcttcg gcgcgcggcc
     2701 agcaagccac cgggccggta gatcatgacg acgatcaaca agagcccgaa gatcgcgatg
     2761 cgtcccgtga gtacgtcgaa cccgagaacg ggttcggctc cggaaaactc acggatgatc
     2821 tccggcaatg cgatcacgat cgcggctccc aggacggcac cggagatact cccgagaccc
     2881 ccgatgacga ccgcgcacag gacgaggatc gactgcagca gcgggaaaga gttcggcgcg
     2941 atgaaagaga tcttcgtcgc gtagatccac cccgccacgc cactggtcga agctccgatg
     3001 acgaacgcaa gcaccttcat cttcgtgacg ggaacgccca ttgccgccgc ggcaacctcg
     3061 tcctcgcgga tcgcagccca gtaacggccg accctcgagt tgtcgaggtg cctgatcatc
     3121 agcaccgcca gaaggatcgc cgggatcagc aggaaccacc agtagtagcg ggtctccagc
     3181 ccgaattcga gatggaggaa cggaaggtcg atcaccgggc tggggatgcc cgggatcccg
     3241 cgggagccgt ttgtgatcgt gaggttgttc gcggtctgtc gcacgatctc gtggaaaccc
     3301 agcgtcacga tggcgaggta gtccccgcgt agtcgaaggg tcggcacgcc tagggtgaac
     3361 ccggcgaaca tcgcgaccag gatgcagaac ggcagcgccg cccacgtcgg ccactcgacc
     3421 aggtggaact ggccgacgtt gcccgagtcc gtcatcagcg caaccgtgta ggcccccagc
     3481 atgaagaagg cgacgtaacc gaggtcgagg agccccgcca ggccgaccac gacgctgaga
     3541 ccaagggcca gcaggatcca caggcccgtg ttgaagagca cctcggtgcc gaagaagcct
     3601 tcgagccgca cccaggagaa cgggggatag ccccgttcca taagaaccgg gatcatcgcc
     3661 atcaagaaag cggccaaggc gtagcgtgcg atcttgagcc cgatgcgggc cgggccgccc
     3721 aatcccgctt cccaatcccg gtatgagccc cgtacccggt cgaactgctc ctggacgacc
     3781 gccatcagcc accgatcctt tcgcccatga ttccggtggg acggaagatg agcaccaagg
     3841 gcagtagcgc gaacgccagc acgttcttcc actcggcgct gatgcagatc gtactcatgt
     3901 tctcgagcaa acccagtagg acggctccca ggaacgctcc tcggatattg ccgatacctc
     3961 cgagtacggc ggaggtgaac gccttgatac ccatgatgaa gcccatgttc cacaccacgt
     4021 tgtcgaaccg gaggccgtac agggcgccgg cgaccccgcc gaagaaaccg ccgatcagga
     4081 acgtggtgac gatcacgcgg tcaacgttga tccccatcag ctgcgcggca acgggatcct
     4141 cggacaacgc gcggatgctg cgtccggtgt tcgtcttatt gatgacccgg tcgaggatga
     4201 cgaagcaaag gatggcaacg accaccagaa ggatgcccgt cgggatcact cgggtgccga
     4261 gaatttggac ggaaaaaccc tcctcgcccg aggcgaaaag tgtcgggaac ccgcgcttgg
     4321 cgggtcccag catgaagttc tgttggaaca ggtaagtcgc gaagaacgag gcgccgatcg
     4381 cgctgatcag gtaggagagg cgagcggcgt tgcgcttacg tagggggcga taggcgacgc
     4441 gttccaggag taccgcaccc ccggccgaaa ccaaaccacc gacgagtaga acgaacagga
     4501 ccatgaacat caactggatc ccggagaagg gcccgttgag gcccaaccat tcgccgatca
     4561 cgaagaacac cgcgatcccg cccagagcga agactgcgtc gtgcgcgaag ttgatcagtt
     4621 tcagaacgcc gtagaccatc gtgtagccca gggccacgag cgcgtacagc aggcccagga
     4681 tgatcccggt aaccagctgt gtccctacaa gcccgaaatt gcagtccaag ttctcccccg
     4741 ccgtccaccc gttggagcgc cgacaagata cccgactagg taaaacgaga gggggaccgt
     4801 aggtccccct ctcttcactt cttggccttg ttcggtgtta cagaagctcc gaagccgggc
     4861 cgagcgacaa gaagttcttc gccttcatgt cccactcgta gatccagacg tcgccgggtc
     4921 caccctcgaa ctcgccgtcg tcgttccacg cgtacgcctt cgtgatcccc tggatcccgt
     4981 ctgcggctgc gaagtagtcc acgaccgcct tacggaccgc agtgatgtcc tcggcgtcac
     5041 cctcgtacgc agcgagggcc tcgatcgcga ggttggtcac gtcggtcatg tcggctgcga
     5101 aggtgcccgg cgcgttcttg ccgtacgcct cctgcatcgc actcacgtag tcctccgcac
     5161 catcgacctt gagcgggtcg gcgcacgggc acgacgcaag agcgccctct gccgcgtcct
     5221 tcgcggtgtc gccgaaggcc gggtccttcg acccgtcgtc cgtcatgaac gtggcatcga
     5281 cacctgcatc cacaagctgc ttcgccagcg gtccggcctg cggcgtgtat ccaccgtaga
     5341 agacgacgtc gggactcgcg tcggcaacct ggcctacgac cgccgagtag tcggtttctt
     5401 cggggttgat cacgtaagtg tcggtgacca ggtcgccgag agccttggcg acaccttcag
     5461 ccagaccctt cgagtagtcc tggttgtcgt gcacgaccgc gacggccttg gccttcagac
     5521 cgtctctgat gtacgcacct gcgacctcgg cctggatgtt gtccggcgcg acggcacgga
     5581 accaggtctc gaagccttgc tcgtcgatcg tctcgttcgt ccccgtaccg gagatcgcga
     5641 cgccggcctt gccaaagatc ttgccggtcg cgagcgtctc accggagaag taaggacagg
     5701 cgcagaacac gacgctctgg ttcgacacga tcttctgtgc gagaccggga gccgggttcg
     5761 ggtcaccctg actgtcctcg gatacgaact cgagctgaca agcgaggttt ccttcttcgt
     5821 tcgctgcgtt aacggcgtac tcgacaccgt cggcgatcgg ctgtccgaca gacgcgtagt
     5881 cacccgacaa cgcacccatc gtcgcgatcg tccaggtgca gtcaccggga ccctcagcag
     5941 cgggaccgcc gccaccgtca ccgccaccgc cgcaagcggc agctacgagg gccatagccg
     6001 cggcagccgc gaccagatga agccttctac tcatgcaatt cccccttcgc caacacgtgc
     6061 cccccttcgg gattgaagag ggggttctct cccagcgtcc aggcgccctg gggaacaggg
     6121 cgcaaacggg cgctagttta cagacgtcac tttcgatttc caatcaccta ctactcctgt
     6181 ggaaccgccg tgacg
//
LOCUS       DATMED010000281         6088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2905224, whole genome shotgun sequence.
ACCESSION   DATMED010000281 DATMED010000000
VERSION     DATMED010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2905224"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VNP73_07165"
     CDS             <1..688
                     /locus_tag="VNP73_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL65736.1"
                     /translation="DKLVGGPGTNLLDGGNDDDNLTGGPDDDVVDTGDGSNDVYAGGG
                     DNRITAGPGNDFIDAAGGDDFIEAGGGSNDVYAGDGDNTITTGSGNDFIDTAGGDDLI
                     EVGGGSNDVYAGGGRNDITSRSGNDFIDTGDGDDLIDSGGGSNDVYSGDGRDTVVTGG
                     ANDFIDTSGGHDVVKSSNGSDDLYGGGGNDRLFGGAGTDFIDGGGGRDEIHGGGGNDD
                     CYDGEKTNSC"
     gene            772..3237
                     /locus_tag="VNP73_07170"
     CDS             772..3237
                     /locus_tag="VNP73_07170"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HWL65737.1"
                     /translation="MSSLLRRRSRAIWILALAATVVAAAGVPIASAQSGPPSPETPVS
                     IEVLSNRADLISGDNALVQINLPSGTGPAEVRVLLNDSDITDDFATRATGDYQGLVTG
                     LELGDNILTATLPNGSGTRIPLTNHPIGGPLFSGPQIQPWYCLAGALDAQCNRPVRYQ
                     YSYKSSVTGNFNPYDPANPPSDLATTTTDEGKTVPYIVREEIGTQDRSQYRIAVLAQP
                     GETWTRWDGPDAWNHKVWVLHGGGCSMAHSEADSPVVLSNTGLSKGFAVMSTALINNT
                     RNCNLVVQAESLMMAKEHLIETYGDLRYMFGHGGSGGAIAQLWIANAYPGLYDGLIVG
                     ATMPDAPVSDILDCVALHRYFEDPRRWAPGVAWTEPNMAAASGKAAISVCRTWITPGP
                     TGYAQVFDPRVGVGCDVPSREPAKAYHPTNNPDGVRCSFQDYLVNILGIRPPSLWGPI
                     EQAIGQGFANRPYDNVGVQYGLRALMAGQITTAQFVDLNTKIGAVDIDYGQQATRVET
                     DPASLETAYRSGLINEGNNLDRVPIIDLPGGANLPGDRYEIHDIYKSWALQERLVASN
                     GHHDNHAIWYGFAEQRQDYFGTMDRWLAAMEADDRDVPLEQKVVENRPANARDACEFP
                     GNRATCDFFFGPGAYSTQWGAGGGIATDVIKCELKPLVLTDYLPIVFSDAQWAQLEDA
                     FPTGVCDWAQPGVGQQGAVAWQTYQGGPGGEPLGEPPVSEPVAPNEDEPLATTLSLAL
                     TKTGSRYLATATLVEASSGAPISGQTIDFLVNGSPAGFALTDAAGRAQITLRSTQLKP
                     RAVLSAVFAGTSDYGASSAQRTR"
     gene            complement(3244..4131)
                     /locus_tag="VNP73_07175"
     CDS             complement(3244..4131)
                     /locus_tag="VNP73_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HWL65738.1"
                     /translation="MREYAVPAGSHPHDVAPAPDGSVWYTAQLTGELGRLDPKTGKTH
                     HIPLGDGSAPHGVIVGPDGAPWITDGGLNAIVRVDPESERVTRWPLPDEAASANLNTA
                     TFDARGVLWFTGQNGIYGRLDPEVGKVETFAAPRGLGPYGITTTPSGDVYYASLAGSH
                     IARIDTKSGKARVIEPPTDGQGARRIWSDSRGRLWVSEWNVGQLAMHDPKASRWSEWR
                     LPGANPAPYAIYVDDSDIVWMSDFGANAIVRFDPETERFKVVRLPSPTSEVRQILGRP
                     GEVWGAASGVDKLIVVRLR"
     gene            complement(4295..5290)
                     /locus_tag="VNP73_07180"
     CDS             complement(4295..5290)
                     /locus_tag="VNP73_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWL65739.1"
                     /translation="MRAVIQDRYGAPGVALHIVDDLNRPAAKEDEVLVKVHASTVTRG
                     EAMGVRFKEYRFTRVFVGIRRPRRPICGMEFAGRVEEVGSGVSRFKVGDEVFGIDNGT
                     CAEYVAVGESSAIEHKPAGMSFEEATAIPDGSLLALTCLKPAYPLEGKNVLVYGAAGS
                     VGTAAVQLLMKHFNAAQVTAVCDTKDVEVLGGLGAHHVIDRFTEDFTTNGKTYDVIFD
                     AVGKHSFRLCRQSLKSRGIYVSMDLGYSYHLLFLGPITKLLGRKRAAVGIGRYRKEDL
                     SLIKDLVEAGKYRPVLDRTYSMDDYVEAATYVESGQKTGNVVIRVAEVGSDGHRD"
     gene            complement(5308..5706)
                     /locus_tag="VNP73_07185"
                     /pseudo
     CDS             complement(5308..5706)
                     /locus_tag="VNP73_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555175.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
     gene            complement(5769..>6088)
                     /locus_tag="VNP73_07190"
     CDS             complement(5769..>6088)
                     /locus_tag="VNP73_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65740.1"
                     /translation="GRRMPDLDLVTANGPLRVYALLHTARPVLLNFGENGGFDITPWA
                     DRVRMIDAKYFGLWELPVLGSVTAPNAVLIRPDGYVGWVGDLTDPGLADALTTWFGPP
                     TAT"
BASE COUNT         1162 a         2012 c         1905 g         1009 t
ORIGIN      
        1 ggacaagctg gtggggggac cgggaacgaa cctcctcgat ggaggcaacg acgatgacaa
       61 cctaaccggg ggcccggacg acgacgtcgt cgacaccggc gacggcagca acgacgtgta
      121 tgcgggcggt ggtgacaacc gcatcaccgc cggtcccggc aacgacttca tcgacgccgc
      181 cgggggcgac gacttcatcg aagccggtgg cggcagcaac gacgtatacg cgggcgacgg
      241 tgacaacacc atcaccaccg ggtccggcaa cgacttcatc gacacagccg gcggcgacga
      301 cttgatcgag gtcggcggag gttcgaacga tgtctacgcc ggtggtggcc ggaacgacat
      361 cacctcgcga tccggcaacg acttcatcga taccggcgac ggcgacgacc tcatcgactc
      421 gggtggcggc agcaacgacg tgtactcggg agatggaaga gacacggtgg ttacaggtgg
      481 cgccaacgac ttcatcgata cctccggtgg tcatgacgtg gtcaaaagct cgaacgggag
      541 cgacgatctt tacggaggcg gcggtaacga ccgactattt ggaggcgccg ggaccgattt
      601 catcgatgga ggcgggggcc gtgacgagat ccacggcggg ggcggcaacg acgattgtta
      661 cgacggcgaa aagaccaaca gctgctgaca cgctcaagaa ggtgaaccgc ctctcttcgg
      721 cgaaatccat aaggccggtt cggcccttct gtaccgaccc taaggagact gatgagctcg
      781 ctgctgcgac gccgatctcg ggctatctgg atcctggcgc tggcagccac cgtggtggcc
      841 gcggccggtg tcccgatcgc cagcgctcag agtggcccgc cctcccctga gacccccgtg
      901 tcgatcgagg ttctgtcgaa ccgcgccgac ctcatctccg gcgataacgc actcgttcag
      961 atcaacctgc cttccggcac aggccccgcc gaggtgcgtg tgctcctcaa tgacagcgac
     1021 atcacggacg acttcgcgac gcgcgccacc ggcgattacc agggactcgt tacggggctc
     1081 gaacttggtg acaacatcct cacggcgacg ctgccgaacg gttccggtac tcgcatcccg
     1141 ctcacgaacc atccgatcgg cggcccgctc ttctccggcc cccagatcca gccgtggtac
     1201 tgccttgccg gcgcgctcga cgctcagtgc aatcgaccgg tcaggtacca gtactcctac
     1261 aagtcgtccg tcaccgggaa cttcaaccct tacgacccgg ccaacccacc ctcggatctc
     1321 gcgacgacga caaccgatga gggcaagaca gtgccgtaca tcgtgcggga agagatcggc
     1381 acccaggacc gcagccagta ccggatcgcg gtgttggcac agccgggcga gacgtggacg
     1441 cgctgggacg ggcctgacgc atggaaccac aaggtgtggg tactgcacgg tggcggctgc
     1501 agcatggcgc acagcgaagc cgactccccc gtcgtcctaa gcaacaccgg tctcagcaaa
     1561 ggcttcgcgg tgatgtccac cgcgctgatc aacaacaccc gcaactgcaa cctcgtcgtg
     1621 caagccgagt cgttgatgat ggccaaggaa catctcatcg agacttacgg cgacctccgc
     1681 tacatgttcg gccatggggg gtcgggcgga gcgatcgccc agctgtggat cgccaacgcc
     1741 tacccgggtc tctacgacgg actgatcgta ggcgcgacga tgccggacgc cccggtcagc
     1801 gacatcctcg actgcgtcgc gctgcaccgc tacttcgagg atccgcggag gtgggcgccg
     1861 ggagtcgcgt ggaccgaacc caacatggcg gccgccagcg gtaaggccgc catctcggtg
     1921 tgccgcacgt ggatcacccc cggccccacc ggctacgcgc aggtgttcga tccgcgcgtc
     1981 ggcgttggct gcgacgttcc gagccgcgag cccgccaagg cttaccaccc gacgaacaac
     2041 cccgacggtg tgcgctgctc gttccaggac tacctcgtga acatcctcgg tatcaggcca
     2101 ccatcactgt gggggccgat cgagcaagcg atcggtcaag gattcgccaa tcgtccgtac
     2161 gacaacgtcg gagttcagta cgggttgcgc gctctaatgg cggggcagat cacaacggct
     2221 cagttcgtcg acctcaacac aaagatcggt gccgtcgaca tcgactacgg acaacaggca
     2281 acgcgggtcg agaccgaccc agcttcgttg gaaacggcat accgcagtgg gttgatcaac
     2341 gagggcaaca accttgaccg cgtgccgatc atcgacctgc ccggtggagc caacctcccg
     2401 ggcgaccggt acgagatcca cgacatctac aagagctggg cattgcagga gcggctggtc
     2461 gcctccaacg gtcaccatga caaccacgcc atctggtacg gcttcgccga gcagcgccag
     2521 gactacttcg gcacgatgga taggtggcta gcggccatgg aggccgacga tcgcgacgtc
     2581 ccgctcgagc agaaggtcgt cgagaacagg ccggccaatg ccagggacgc atgcgagttc
     2641 cccggcaacc gcgccacatg cgacttcttc ttcggccccg gcgcctacag cactcagtgg
     2701 ggcgccggcg gcggcatcgc gacggatgtg atcaagtgcg agctcaagcc gctggtgcta
     2761 accgactacc tcccgatcgt tttcagtgac gcccagtggg cgcaactcga ggatgccttc
     2821 ccaaccggtg tctgcgattg ggcccaaccc ggtgtcggcc agcaaggcgc ggtcgcgtgg
     2881 cagacctacc aggggggccc gggcggtgaa cctctagggg agccgccggt gtcggagccc
     2941 gttgcaccga acgaagacga gccgctggcg acaacgttga gcctggcgtt gaccaaaaca
     3001 ggcagccgct acctcgctac ggccaccctg gtcgaagctt cgtcgggagc accgatctca
     3061 ggtcagacga tcgatttctt ggttaacggc tcaccggccg gcttcgcgct cacggatgcc
     3121 gcaggccgag cacagatcac actgcgttcg acccagctga aaccacgggc tgtgctgagc
     3181 gcggtgttcg ccggaacgag cgattacgga gcttcgtcgg cccaacgcac gaggtagcaa
     3241 ggctcagcga aggcgaacga cgatcagctt gtccactccc gacgcggccc cccacacctc
     3301 gcccggccgc ccgaggatct gacgcacctc tgaggtggga ctcggaaggc gaacgacctt
     3361 gaagcgttca gtttccgggt cgaagcgaac gatcgcgttc gccccgaagt cgctcatcca
     3421 cacgatgtcg ctgtcgtcga cgtatatcgc gtatggagca ggattggccc cggggagccg
     3481 ccactcactc cacctcgaag ccttggggtc gtgcatggcg agttgcccga cgttccactc
     3541 actcacccat aagcgtccgc gcgaatcgga ccagatgcgg cgagcacctt gaccatccgt
     3601 gggcggttcg atgactctcg ccttgcccga ctttgtgtct atgcgcgcga tgtggctgcc
     3661 cgcgagggat gcgtagtaga cgtcgccgct cggcgtggtt gtgatgccgt aaggccccag
     3721 gccccgaggg gccgcgaacg tctcgacctt gcccacctcg ggatcaagac gcccgtagat
     3781 cccgttctgg cccgtgaacc acaacacacc gcgagcgtcg aaggttgcgg tgttgaggtt
     3841 ggccgatgcc gcctcgtctg gaagcggcca tcgggtcacc cgttcgctct ctgggtcaac
     3901 ccgaacgatc gcgttgagcc cgccgtcggt gatccacggc gcgccgtccg ggccgacgat
     3961 gaccccgtga ggcgcggatc catcgcctaa cgggatgtgg tgggtcttgc cggtctttgg
     4021 atccaaacgc cccagctcgc ccgtgagctg agcggtgtac cagacggaac cgtcgggagc
     4081 cggtgccacg tcatgcgggt gagagcctgc aggcacggcg tactcacgca caccgacgac
     4141 cttcgagcta ccgcctggct cgtcgcgccg ttcgcgttgg gttggatcgt cggcgacggt
     4201 ggtgcttggc gtatcagtag cggagggcgt cgtcgtgtct gatgtgcatg ccgtcgacaa
     4261 aacgaggacc gtccccaacc aaacggcgcc tcttctaatc acggtgtcca tcagatccca
     4321 cttcggcaac gcggatgaca acattgccgg ttttctggcc ggactcaacg taggtcgctg
     4381 cttcgacgta gtcgtccatc gagtacgtac ggtcgagcac cggccggtac ttccctgcct
     4441 cgaccagatc cttgatcagc gacagatctt ctttgcgata gcggccgatg ccgaccgctg
     4501 cgcgtttccg gcccaacagc ttggtgatgg gccccaggaa caacagatgg tagctgtagc
     4561 cgagatccat cgagacgtag atgccgcgag atttcaacga ctggcggcag aggcggaacg
     4621 aatgcttacc gaccgcgtcg aagatcacgt cgtaggtctt cccgttggta gtgaaatcct
     4681 cggtgaagcg atcgatgacg tggtgagcgc caagcccccc aaggacctcg acgtctttcg
     4741 tgtcacacac cgcggtcact tgggcggcgt tgaagtgctt catgagcaac tggaccgccg
     4801 cggttccgac agaaccggcg gcgccataca cgaggacgtt ctttccctcc agtgggtagg
     4861 cgggcttcag acaggtaagc gccagcaggg agccgtcggg tatcgcggtt gcttcctcga
     4921 aactcatgcc ggcgggcttg tgttcgatcg cactgctctc accgacggcg acgtattcgg
     4981 cgcacgtacc gttgtcgatg ccgaacacct cgtcgccgac cttgaatctg gatacgccag
     5041 atccgacctc ctcgacgcga cccgcgaact ccatgccgca gatcggtcgt cgtgggcggc
     5101 ggataccgac gaatacgcgt gtgaagcggt actccttgaa acgcacgccc atcgcttcgc
     5161 cgcgggtgac ggtcgaggcg tggaccttga cgaggacctc gtcctccttg gcggcaggac
     5221 gatttaggtc gtcaacgatg tgtagcgcga cccctggagc tccgtagcga tcctggatga
     5281 ctgctctcat ttgaggtgac gggtgactca gccctttgga ctccgaacga cggcggtcgc
     5341 cgtcgccgtc gccacgaccg ctccagactc gtcaaccaat tcgcccgcca ggaatccgac
     5401 gccggaacct cgacggacga tacgtccgcg cccgaccaga cgtccgaccc gagccggctt
     5461 aaggaactgc acatgaaggt cggtcgtcgg cgcgaattca cccggcccca gagtcgccac
     5521 caatgccggt cccagcgtgt catccagcat cgctgcaagg aatcctccct gcacgacgcc
     5581 gaccgggttc aagaactgtt ccgtcgcagt gaacgccacc tcgatggtgc ccgcgtccgg
     5641 atcgacggac agcagctccc acccaagcta caccgcagcc ggcggcggct ccgccctgcc
     5701 ttccatgacg tcccagaacg ggccctgtct cgacaccacg tcttcctatc agggcggtgg
     5761 ggcgtgcgct acgtcgcagt cggcggtccg aaccacgtgg tgagcgcgtc ggcgagcccg
     5821 ggatcggtca ggtctcccac ccagccgaca taaccgtcgg gccggatcaa caccgcattc
     5881 ggagcagtga ccgagccgag caccggaagc tcccacagac caaaatattt ggcgtcgatc
     5941 atccgaaccc gatccgccca cggtgtgatg tcgaagccgc cgttctcacc gaagttgagc
     6001 agcacgggcc gtgcagtgtg cagcagggcg tacacgcgca gcgggccgtt agcggtgacc
     6061 aagtcgaggt cgggcatgcg ccgcccga
//
LOCUS       DATMED010000282         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_226393, whole genome shotgun sequence.
ACCESSION   DATMED010000282 DATMED010000000
VERSION     DATMED010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226393"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VNP73_07195"
     CDS             <1..1473
                     /locus_tag="VNP73_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HWL65741.1"
                     /translation="DSPDERAAAPVEDGARSLAGNSLWALTAQAVSKVASVVFVAAMA
                     RALSKVDYGYFNLALSLVPLFLIFGIWSLDAVVIKEIARNPTRTATVFASGLVARAGL
                     GALALGMAVLVTPFFVDGMTPVLAIALVGAALFLDQIAEFIGAVFRGLERMSLYGIVI
                     MANRIVSTAITLVVLARGGTLVAASSAYLVGSLIALAFAWILLLRITPIHRTDVSSNE
                     TKRLVREGLPLGVSDVLNMAILRLDSVLLYLLRGPVSVAVYGVAYRLFESLLFVAWSM
                     ATAALPRIARAHTPADSARTYDLLLTATLTIYVPAAAITPFAGSWLVTTVFSDRYLPS
                     AEALTWLMLALVLYGFGHMARVAAIGIGRGREIARVAGASLAVNIVANLTLIPRFGFV
                     GAAMATTITEGCQAVALGTLFYRATATRPSVKILMPLAAGAATAGVLTALNAAGFAAI
                     VIGMGTYAITLLGISAVLAPELGASLLRLIMKRRVGHGSS"
     gene            1470..2507
                     /locus_tag="VNP73_07200"
     CDS             1470..2507
                     /locus_tag="VNP73_07200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL65742.1"
                     /translation="MSESITIHRSIDEISHEWNALAEKLKTEPWMRPAWFRIWQEAFG
                     RGELEIHTVRDEGVLRGLVVMERIGSELRSAAATETPSHGFLTESDSESRLLAQSLLE
                     AADRVNLWPIPAHDASILRDGALAAGRSLLSRDIGGSPFVRISDWEAYSAGLETKMLR
                     ELRRRKRRLDERGDLTLEVTTGRDEWGDLFDECLTLEGRGWKGESGTAIASNIATRTF
                     YRRLCMEASDSDWLVIGSLRLDERLIAFDISLELDERHHLMKTSFDPELARYSPGSQL
                     RYLMLRRAFDLQLQTYEFLGRDDAWKMEWTSEVRPLQHVRTFPGTLRGSAERIAYRVG
                     RVVKRRLARSQ"
     gene            2600..>3383
                     /locus_tag="VNP73_07205"
     CDS             2600..>3383
                     /locus_tag="VNP73_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWL65743.1"
                     /translation="MALFKLGRHCLWHGLRSIGLDAGGVLVPAYHHGSEIEVLRVAGL
                     DLRFYDVTDSLEPNVDELTDSDLDGVRALYLIHYLGFPQNAGKWRRWCDDRGLLLIED
                     VAQGWLGNSDGRPLGAYGDVSIFCLYKQLGVPDGGALATTGDVSSVATRSLGVGRLFR
                     RHAAWASSRSAALARLTPPHVDRPKEAIADAEFRLGDPWTPPSGATNYLLPRLCHPAV
                     AEMRRDNYRYLADRLGDLIAPGFDGLPEGASPFAFPIAVGAKD"
BASE COUNT          568 a         1065 c         1045 g          705 t
ORIGIN      
        1 gatagccccg acgaacgtgc cgctgccccc gtggaagacg gcgcccgatc cctagccggc
       61 aacagcctgt gggccctcac tgctcaagcc gtcagcaagg tggcttcggt cgttttcgta
      121 gctgcgatgg cgcgggcact gtcgaaagtc gactacggct acttcaatct ggccctctcg
      181 ctcgtccccc tcttcctcat cttcggcatc tggagtctcg atgcggtcgt gatcaaagag
      241 atcgctcgca acccaacccg cacggcaacg gtcttcgcaa gcggtttggt ggcgagagct
      301 ggactcggcg cgctggcgtt ggggatggcc gttctggtga caccgttctt cgtcgacggg
      361 atgacgccgg ttctggcgat cgcgctggtg ggggccgctc tcttcctcga tcagatcgcc
      421 gaattcatcg gcgccgtctt cagaggactc gaacggatgt ctctgtacgg gatcgtcatc
      481 atggccaatc ggatcgtgtc gaccgccatc actctcgtgg tcctggctcg tggcggaaca
      541 ctggtcgcgg catcctcggc ctatctcgtc ggatcgctga tagcactggc gttcgcgtgg
      601 atccttctgt tgcgaatcac tccgatccac cgaacggacg tgtcctccaa cgaaacgaaa
      661 cgtttggttc gggaaggact gccgctgggc gtctcagacg tcttgaacat ggcgatcctg
      721 agattggact cggttctctt gtaccttctg cgcggcccgg tctccgtcgc cgtttacggc
      781 gtcgcctatc gcttgttcga gtcgctgcta ttcgtcgctt ggagtatggc aaccgcggct
      841 ctcccgagga tcgctcgagc gcacaccccg gccgactcgg cgcgaacgta cgaccttctg
      901 ctcacggcaa cactcacgat ctacgtacct gcagcggcga tcactccgtt cgccggcagc
      961 tggctcgtta caacggtctt ctcggaccgt tacctcccct ccgccgaagc gctgacctgg
     1021 ctgatgcttg cattggtcct ttacggcttc ggccatatgg cccgggttgc cgccatcggc
     1081 atcggtcgcg gccgggagat agctcgcgtt gccggagcat cgctcgccgt gaacatcgtc
     1141 gcgaacctga cattgatccc ccgcttcggg ttcgtgggag ctgcgatggc caccaccatt
     1201 accgagggct gtcaggccgt ggcgctcggt acgctgttct acagagcgac tgccacgcgt
     1261 ccctcggtaa agatcctgat gccgctcgcg gccggggcgg caacagctgg tgtgctgacg
     1321 gctctcaacg cggcggggtt tgcagcgatc gtgatcggga tgggtacata cgcgatcacc
     1381 ttgctgggga tctcggccgt actcgcgcca gaactcggcg cgagtctgct gcgcctcatc
     1441 atgaaacgaa gggtgggaca cggttcttca tgagcgaatc gatcacgatc catcggtcga
     1501 tagacgagat ctctcacgag tggaacgcgc tcgcagagaa gctcaagacg gagccctgga
     1561 tgcgcccggc ctggttccgc atctggcaag aggcgttcgg cagaggcgaa ctcgagatcc
     1621 ataccgtacg cgatgagggc gtcttgagag gcttggtggt gatggagcgc atcggttcag
     1681 aattgcgctc cgccgccgcc acagaaacgc ccagtcatgg tttcctgacc gaaagtgact
     1741 cggagtcgag gctcctcgct cagtctctgc ttgaggcagc cgaccgggtc aatctctggc
     1801 ccattcccgc tcacgacgca tcgatccttc gcgacggcgc attagccgcc ggacgttcgc
     1861 ttctctcgcg ggatatcgga ggctccccat tcgttcggat ctccgactgg gaggcttact
     1921 ccgccggtct cgaaacaaag atgctgcggg aactccgcag gaggaaacgc agattggacg
     1981 aacgaggtga tctcactttg gaagtaacca cgggccgtga cgagtggggt gatctgttcg
     2041 atgaatgcct gacgctcgag ggccgtggct ggaaaggtga atcgggcacg gccatcgcgt
     2101 cgaacattgc aacgcgaacc ttttaccgtc gcctctgcat ggaagcctcg gacagtgatt
     2161 ggttggtgat cggctctttg cggctcgacg agagactcat agcgttcgat atctcgctcg
     2221 agctcgacga gaggcatcac ctgatgaaaa cgagtttcga ccccgagctg gcgcggtatt
     2281 cgccgggatc gcagctgagg tatctcatgc tccgcagggc attcgatcta caactgcaga
     2341 cctacgagtt cctcggtcgc gacgacgcat ggaagatgga atggacttcc gaggttcggc
     2401 cgcttcaaca cgttcggacc ttccccggca cgttgcgcgg ctctgccgag cgcatcgcct
     2461 accgggtggg gcgggtcgtc aagcgtcgtc tagcgagatc gcaatgagtt ccggtcgtct
     2521 cgtagtcatc ccacccctga gcccgcgagc tcatctgacg cgcccacgtc gtctgccatt
     2581 tcccttcgga acttccaacg tggcgctgtt caaactgggt cgccactgtc tgtggcacgg
     2641 attgcgatcg atcggtctgg atgctggagg agtgctcgtg cccgcgtacc accacgggtc
     2701 ggagatcgag gttctccggg tggccggact ggatcttcgc ttctacgacg taacggattc
     2761 gttggaaccg aacgtcgatg aactgacgga ctcggacctc gatggggtgc gcgccttgta
     2821 cctcatccac tacctgggct ttccgcagaa cgcgggaaag tggcgccggt ggtgcgatga
     2881 ccgcggcctg ctattgatcg aagacgtcgc tcaggggtgg ctcgggaact cagacggtcg
     2941 ccccttgggt gcgtacggcg acgtgtccat cttctgtctc tacaagcagc tcggcgtacc
     3001 cgacggaggc gcccttgcaa caacgggcga cgtttcgtcc gtcgccactc ggtcgttggg
     3061 agttggtcgc ctgttccggc gtcatgcggc gtgggcctcg tcccgcagcg ccgcgttggc
     3121 ccggttgacg ccgccccacg tggatcgacc caaagaagcc atcgcagacg ccgaattccg
     3181 gctcggtgat ccgtggacac cgccgtcggg cgcgacgaac tacctgcttc cccgcctctg
     3241 ccaccccgcc gtagcggaga tgcgtcgtga caactaccga tatctcgcag accggttggg
     3301 tgatctcatc gccccaggct tcgacggtct ccccgaaggg gcgtccccgt tcgcattccc
     3361 aattgcggtc ggcgccaagg act
//
LOCUS       DATMED010000283         5215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4824251, whole genome shotgun sequence.
ACCESSION   DATMED010000283 DATMED010000000
VERSION     DATMED010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4824251"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VNP73_07210"
     CDS             complement(<1..285)
                     /locus_tag="VNP73_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWL65744.1"
                     /translation="MTRRAAAAVLLVALVSCVGSRDDPPEHPNVLVIVTDDQRARGTL
                     AVMPSVRRWFVREGTVFENAFTTTPLCCPARASLFTGRYVHNHGVRHNHQS"
     gene            complement(282..1898)
                     /locus_tag="VNP73_07215"
     CDS             complement(282..1898)
                     /locus_tag="VNP73_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65745.1"
                     /translation="MAIVSLAVLCPLAVISAYALARSLRASEPAAAAVASYDARACSL
                     GPKILGRIRRGYIQNRSEDIVFVLRGKNYLGSFKTISHSGPRDFLQRVPLVLYGPRIE
                     AEGVLDRPVTLADVYPTVGQLMDIELPERRGGVLSEALVGTAGIPRLLVVVMWDGVGR
                     NVLDRWPGAWPTLARLEADGTSFLEATVGSSPSITPATHSTLGTGTFPRNHGVTSIKF
                     RRENGAIGESFKRRNPRELRLSTFADEIDLAFDNQPQVGMLAWRNWHMGMLGHGATLE
                     GGDRDHLGLITRRSRITGRREFFFTPRYLDGFPGLDRLADELDARDGVKDGRWRGHDI
                     RADNDNPAWVAYQAKVLHALAKREGYGANETPDVLLTNFKITDIVGHKYSMDSPEMKD
                     VLRAQDDALGKLIGYLDSEVEDYVVIVSADHGHTPSAESSGAWPLVQHELEADLDRRF
                     DVTGSTLARSVSAVGVWVNRGVMDEHNITYDEIATFLNGYTIRDNWGRSQLPSAYRDR
                     GAEPVFDAAFPGTKLDEAVECAASQSTSAP"
     gene            complement(1940..4225)
                     /locus_tag="VNP73_07220"
     CDS             complement(1940..4225)
                     /locus_tag="VNP73_07220"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWL65746.1"
                     /translation="MAVQRGQLRDPQLTARYLVPIAAVILLVALMGGTSPGELIFFAA
                     TAAALSYALDAVAGPARISMPARVAQGVITALLLAIPLLVDPEAFALYDIPKFTALVV
                     GTCLTLIVVCLRRLAGGTQLVIRNGLELPIAGLVGALTLATIFASSITTGILGLYASL
                     DGLITTLACAGLLFATVHVFTIADVRRLLKVFFFGATGVILLYGLLQFIDISLGGGWE
                     FIPTKGDLGLSATLSNKNHLSGFLAAMLPVGVALVITARSRWDRMAGLFALAILILEL
                     SFNRTTGAFVGAVLGLLVLGWYQRRALVEAWRGVSPTWKWMFAGVAGLLLVAVGVFML
                     LRLRGVVAEQDASLVRAAGGRLQYWSAALKMAGDDPITGVGPDSFMVHFWSYQTRPFV
                     EAFGPVRGVNGAHNIFFNYLATTGFVGLSAFVALIFFLFLRGTAAIRRLRVIGTQSKE
                     GTEATVLVSASLGAIAGYLGQATFNIQHVELTFVFWMMAALVCLAALDTGVPSSARPR
                     EVLRPAVQQEEEDRSKSKKKSRKGGTGTELPVAPIRTIRTSATPAGRAGIWAFCVLCI
                     AVFWFGLRPLRADHAYRDGLGLEDSLGQYENEERVRVLAAAYESFEDAWTLNPWEARY
                     ADNAGRVRLRFPIDLREGTFEQSDAAAVEQQFVAAIEWMDKGLEVQPHDALLWASRGE
                     AFFRLSSISDDERHRSEAISSLNRSLELNPWLIDVHQLLVQVYVDADMLDEARDAFAT
                     ASLYATEEDDLDEIEAELETL"
     gene            complement(4272..>5215)
                     /locus_tag="VNP73_07225"
     CDS             complement(4272..>5215)
                     /locus_tag="VNP73_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65747.1"
                     /translation="FMFDEDIAGDPVEDQFQIVAEDGTKTLAAAGTCDIDNDSDVTCD
                     YPAIEDAGDNEIVVAGVGDCAVEADADAECNTVGDVPITDSLRAPGLSDAPDLESCDI
                     DEASNLATFFFDEQVDEDSADESAFDVFDVSGNLSSASEVDDSNEEEGEITLEFDEDA
                     VEDAVGCHVDFDAVDDFLTNASPEATVGTAQPAPTTATTTTTPITSDTTSPTPTTRHV
                     QASISISFDRPAFKGTIRSGRAGCERGRRVVLKKKKRGVNPRAGSDTSDRAGNWRIGR
                     RNASGRYFAVAQRKVFTAANGDRIICDPKTSDTRRVR"
BASE COUNT         1032 a         1647 c         1587 g          949 t
ORIGIN      
        1 cgactggtgg ttgtgtcgca ctccgtggtt gtggacgtag cgaccagtga agagcgatgc
       61 acgagcgggg cagcacagag gggtggtcgt gaaggcattc tcgaacactg tcccttcgcg
      121 aacgaaccag cggcggaccg acggcatcac cgcgagcgtg ccgcgagctc gttgatcgtc
      181 cgtgacgatc acgaggacgt tcgggtgctc gggcgggtca tcccgcgatc cgacacagga
      241 aacgagagcg acgaggagca ccgcagctgc ggcgcgacgg gtcacggagc ggaggtggac
      301 tgcgaggccg cacattcgac ggcttcatcc agcttggttc cggggaatgc agcatcgaac
      361 acgggctcgg cccccctatc ccggtaggcg gacgggagct gggatcgccc ccagttgtcc
      421 cggatcgtgt agccgttcaa gaacgttgcg atctcgtcgt aagtgatgtt gtgctcgtcc
      481 ataactcctc ggttgaccca caccccaacg gcacttaccg agcgggcgag ggtcgatccg
      541 gttacgtcga accgacggtc gagatcggct tccagttcgt gctgaacgag tggccatgca
      601 ccgcttgact cggccgacgg cgtgtggccg tgatccgcgc tcacgatcac cacgtaatcc
      661 tcgacctcgg aatcgaggta accgatcagc ttccccagag catcgtcctg ggcgcgcagg
      721 acgtctttca tctcggggga gtccatcgag tacttgtgac ccacgatgtc cgtgatcttg
      781 aagttcgtca gcaggacgtc gggcgtctcg ttcgctccat aaccttcgcg cttggccagg
      841 gcgtgaagga ccttcgcctg gtaagccacc cacgcgggat tgtcgttatc cgccctgatg
      901 tcgtggcccc tccacctgcc gtccttcacg ccatcgcggg cgtcgagctc gtcggccaac
      961 cgatccagac cggggaaacc atccaggtag cgcggggtga agaaaaactc gcggcggccc
     1021 gtgatgcggc tcctgcgcgt gatcaaaccg agatggtccc ggtcccctcc ctcgagcgtc
     1081 gccccgtgtc ccaacatgcc catatgccag ttgcgccacg caagcatccc gacctgaggt
     1141 tggttgtcga atgcgaggtc gatctcgtct gcgaacgtcg acaggcgaag ctctctcggg
     1201 ttgcgcctct tgaaagactc tccgatagct ccgttctccc tgcggaactt gatggatgtg
     1261 acgccgtgat tgcgcgggaa ggtgccggtt cccaatgtcg aatgggtggc aggcgtgatc
     1321 gaaggcgacg atccgaccgt tgcctccagg aaagaagtgc cgtcggcctc cagacgcgcc
     1381 agtgtcggcc atgccccagg ccaccggtcg agcacgtttc tgcctacgcc gtcccacatg
     1441 acgaccacga gcagacgagg gatccctgcg gtccccacga gcgcttccga gagaacgccg
     1501 cccctgcgtt cgggtagttc gatatccatc agctgcccga ccgtcggata gacgtctgcg
     1561 agcgtgaccg gcctatccag gaccccttcc gcctcgatcc gcggcccgta gaggacgaga
     1621 gggacgcgtt gtaggaagtc ccgcggtcca gagtgactga tcgttttgaa cgacccgagg
     1681 tagttcttgc ctcggagcac gaacacgatg tcttcggatc ggttctggat gtagccgcgg
     1741 cggatccgtc cgaggatctt cggacccaat gagcacgcgc gggcgtcgta ggaagcaacg
     1801 gcggcggcgg ccggctccga ggccctcaaa ctgcgagcga gcgcataggc ggagatgacc
     1861 gccagcggac acagaacggc cagagagacg atcgccatca cgaaccgccc taccaagtga
     1921 tcgcctccag cgcctttcgt cacagcgttt ccagttcggc ttctatctcg tcgaggtcat
     1981 cctcttcagt ggcgtacaga gacgcggtcg caaaggcgtc gcgcgcttcg tcgagcatgt
     2041 ccgcgtcgac gtagacctgt accaggagtt ggtgaacgtc aatgagccag ggattcagtt
     2101 ccagggagcg attaagggat gagatcgcct ccgaccgatg cctctcgtca tcgcttatcg
     2161 aagacaaccg gaagaacgcc tcaccccgac tggcccagag gagtgcatcg tgcggttgca
     2221 cttcaagacc cttgtccatc cactcgatcg ccgccacaaa ctgctgttcc accgccgcag
     2281 catcagactg ttcgaaagta ccctccctga gatcgatcgg aaaccggagc cgcacccggc
     2341 ccgcgttgtc cgcataacgc gcctcccacg ggttaagggt ccaggcatcc tcgaacgact
     2401 cgtatgccgc ggcgaggaca cgcacccgct cctcattttc gtactgcccg agcgaatcct
     2461 ccagtccgag cccgtcgcgg taagcatggt ccgctcggag tggtctgagt ccgaaccaaa
     2521 acacagcgat gcaaaggaca cagaacgccc atatccccgc gcgacctgcc ggtgtggctg
     2581 aggtccgtat ggtccggatc ggagccaccg gaagctccgt gccggtccct cccttccttg
     2641 acttcttctt gctttttgat cggtcctctt cttcttgttg gacggcaggt ctaaggacct
     2701 cccttggtcg ggccgaagag ggaacaccgg tgtcgagcgc agccagacaa accaatgctg
     2761 ccatcatcca aaacacgaat gtcagctcga catgttggat gttgaacgtt gcctgcccca
     2821 gataccccgc gatggcccca aggcttgcgg acacgaggac ggtcgcctcg gtgccttctt
     2881 tcgactgagt gccgatcacc cgcaagcgac gaatggccgc cgtacctcgt aggaagagaa
     2941 agaagatgag tgcaacgaag gccgacaggc cgacgaaacc cgttgttgcc agatagttga
     3001 aaaagatgtt gtgagcaccg ttcacacctc gaacgggtcc gaaagcctcg acgaacggcc
     3061 gagtctggta actccagaag tgcaccatga aggaatcggg tcctacgccc gttatgggat
     3121 catcccccgc catcttcaaa gcggcgctcc agtattggag acgacctccc gcggctctga
     3181 cgagagacgc gtcctgttcg gcaactaccc ctcggagacg gagcagcatg aacaccccca
     3241 cggcaaccag caaaagtccc gcaactccgg cgaacatcca cttccaggtc ggcgagactc
     3301 cacgccacgc ttcgaccagt gcacgacgct gataccaacc cagcaccagt aaccccagta
     3361 ctgcgcccac gaatgctccc gttgttcggt tgaacgagag ctctaggatg aggatggcga
     3421 gcgcaaatag tcctgccatc cggtcccacc ggcttctggc cgtgatgacc agtgctacgc
     3481 cgaccggaag catcgccgcc aggaaccctg agaggtgatt cttgttagac agggtggcgg
     3541 agagaccaag gtctcccttg gtggggatga actcccatcc gcctcccagc gagatgtcga
     3601 tgaattgaag tagtccgtac agaaggatga cgccggtggc tccgaagaag aagaccttaa
     3661 ggagtctcct gacatctgcg atcgtaaaaa catgcacggt tgcgaacaag agaccggcgc
     3721 aagccagggt tgtaatcaac ccgtccagag acgcgtagag gcccaggatc cccgtcgtga
     3781 tgctcgaagc gaagatggtc gcaagagtta gggcgccgac gagaccagcg atgggaagct
     3841 cgaggccgtt ccggataacg agctgcgtcc cgccggccaa tcgtcgtaag caaacgacga
     3901 tgagagtcag gcatgttccg acaaccagag ccgtgaactt cgggatgtcg taaagagcga
     3961 aagcctcggg gtcaacgagg agaggtatag ccaacaggag cgctgtgatc actccttgcg
     4021 ccactcgagc gggcatcgag atgcgagccg ggcccgctac ggcgtctaac gcgtacgaga
     4081 gggccgccgc cgtcgccgcg aagaagatga gctcgccggg ggatgttccg cccatgagag
     4141 caaccagaag aatcacggcc gcgatgggaa ccagatagcg cgccgtcagc tgagggtcac
     4201 gaagctgccc gcgttgaact gccattgccg aatcctatga ggcgccgggg ggaaccccgg
     4261 ctaaaccact actacctaac gcgtctcgtg tcggaggtct tggggtcgca gatgatcctg
     4321 tcaccgttcg ccgcggtgaa aacttttcgc tgggccacgg cgaagtaccg accggacgcg
     4381 ttgcgccgac cgatcctcca gttacctgct cgatcggagg tgtcgcttcc cgcccgtggg
     4441 ttcactcctc gcttcttctt cttgagcacg acccgtcgac ccctctcgca acctgcccgg
     4501 cccgacctga tcgttccttt gaatgcgggg cgatcgaacg agatcgagat gctggcctgg
     4561 acgtggcgag tcgtcggcgt cggcgacgtg gtgtcagacg tgatgggggt cgtggtcgtc
     4621 gttgccgtgg tcggcgccgg ctgagccgta ccgacggtgg cctccggcga tgcgttggtg
     4681 aggaagtcgt cgaccgcgtc gaagtcgacg tgacaaccca ccgcgtcctc gaccgcgtcc
     4741 tcgtcgaact cgagcgtgat ctcgccctct tcctcgttcg agtcgtcgac ctcggaggcc
     4801 gaggagagat tgccgctcac atcgaataca tcgaaagcgc tctcgtccgc ggaatcctcg
     4861 tccacctgct cgtcgaagaa gaacgtcgca aggttcgagg cttcgtcgat gtcgcagctc
     4921 tcgaggtcgg gagcatcgct tagaccgggg gcccgaagag aatcggtgat cgggacgtcg
     4981 ccgaccgtgt tgcattcggc gtcggcatcc gcctcaaccg cgcagtcccc tacgccggcg
     5041 acgacgatct cgttgtcccc ggcatcctcg atcgccgggt agtcacacgt gacatcgctg
     5101 tcgttgtcga tgtcgcatgt gccggcggct gccaatgtct tcgtcccgtc ctcggccacg
     5161 atctggaact ggtcctcaac cggatcgccg gcgatgtcct catcgaacat gaagg
//
LOCUS       DATMED010000284         6089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4440519, whole genome shotgun sequence.
ACCESSION   DATMED010000284 DATMED010000000
VERSION     DATMED010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4440519"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="VNP73_07230"
     CDS             complement(<1..2231)
                     /locus_tag="VNP73_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWL65748.1"
                     /translation="MSDQHLGTQLGGYEIESLLGRGGMGVVYKARHLRLGRMVALKIL
                     APELAADDNFRTRFVRESQMAASIDHPNVIPVYDADEIDGTLFIAMRYVDGTDLKKVI
                     EADGALTPERTLTVISAMAGALDAAHALGLIHRDVKPANVLVGRTETTPEQIYLTDFG
                     LTKHSGSKSGLTATGAFVGTIDYIAPEQIEGKTVGTQADIYALGCVLFECLTGSVPFE
                     KEADVAVMYAHLMDDRPKATDKNASLPAAVDAVLAKAMAREPEARYGSGAELVDDLGS
                     ALGGGAAAPVAVATPPPAPVEPKAPEIRVEVPPEPAGAPAARRISPKMAAVALGAVIL
                     VAGVAFFMTRDGDDVKETEPTSGSGIPADAVLVEPGGDLRAAVAKAGEGGTVILADGF
                     YESKKVPMVTIKQSVTIMGAEGAAPVLRGNDRFPDGIRIAAGTSDVTVSNLIFDNFNG
                     EALKVFCFTCEDGIENQNITFENLEFRSGGSPIKVENVSGLSLEGVTVQENDFVGLLC
                     DPGPCEDVTIKNSMFQSGVQPGSAGVLIESGLNVLIEDSDASFNGGSGFVSSAANTQI
                     VRSTATNNRFDGADLYGANSEIRDSIVADNRTGAVEMGEGDCKRSCAGGVFTIANSLM
                     EGNGSGIFVSGDSGGTQFQMFNSILTDNRNDAVNVAGAVKLIRIDHNLIHSVGYPALI
                     YKGKKYTSESINNLPINTDETYGYAPVFVAPDDYHLTPESDGVDAGTQEGVQSTVDLD
                     GNPR"
     gene            complement(2228..5176)
                     /locus_tag="VNP73_07235"
     CDS             complement(2228..5176)
                     /locus_tag="VNP73_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWL65749.1"
                     /translation="MALGRQELGMFSSVVDKGHTRRESVEMTGDEGGATPSEAPAPSR
                     TSDELKPITALFADVVGSTSLGEKLAPDEVKALIGECVTRMAAAVEEFGGFVQAFMGD
                     GICAYFGVPTAHEDDPERAARAALRIREDVATYAADVEAAWGISDFNVRVGINSGQTA
                     VGLVGASDPQAVALGDTTNTAARLQSAADPGSIAVGEITARLLDGAFVLEEAGAVAVK
                     GKAESVPFWRLIEARTSEHARSIGRLFGRDGEIQILKRAADDLVDGRGQVVAISGDPG
                     LGKTRMMGELHNLLGDRATWLEGRCLSYGGELPYWPFVQTVRDWLGVRDGEADIAVRT
                     KLRAHLQPLMGAVAEEVLPYLAKLLSLPGDPKQKELFDSLDRAELNARIGGAYVTWIE
                     AMCTSKPVALALDDAHWIDPASQEVLTQLLGLTDRAPLMLVMTLRLDLDGSGRGIRAA
                     VLEDFPHRMTDMPLLPLDTQDARALADHLAPEVGLDEASCETIVNLAEGNPLYIEELV
                     TALIEGGELVRQRTWTLSSRTEDLLPPALESLLVARIDRLKEGARRLAQIAAVIGRTF
                     RVDVLREVAEGPDLDADLAELLRAQIVREVRRFPTLECTFKHGLVQEAAFSSLTPQRR
                     RELYGRVAAVFEHLDGANRDEHLPLLAFYYYRSDDRAKALEYLEAAAKRAEQLDPDQA
                     AELWARARKVAAKVSDKSAVPTTSPESPAATASTETIGGFVVDEFLGDGATGTVYRAT
                     SADGEQVALKILKPELAEDQVFRRRFEHEVRAAREVDNEHLVAIRDAGEADGALYIAM
                     EFLEGRPLNEIVERDGPLDVRGTVRVVLQVGAALDALHGRDIVHRDVKPSNIVLAERG
                     AVLTDLGLAKGRAYTVLTRPGQVLGTLDYMAPEIIKGEEAGPASDIYALACVAYECIT
                     GRPPFADKGVFQVGKAHVVDEPPDASAARSDIPKEFGWAISQALAKDPARRPPSGIAF
                     ANLLKMAAKS"
     gene            complement(5226..>6089)
                     /locus_tag="VNP73_07240"
     CDS             complement(5226..>6089)
                     /locus_tag="VNP73_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65750.1"
                     /translation="EDIDIQFSLAPDIRAIEMDPSQFDQILLNLAVNARDAMPGGGIL
                     MIETSHVRLMEGGSWDGEEVPAGEYVCIAVSDTGTGMSEEVRSRIFEPFYTTKERGKG
                     TGLGLATVYGVVKKLGGTISVYSEPGVGTTFKLYLPVATKDAELVIEERSDAARSGEG
                     CTVLVVEDEGGVRRVAHRILAKAGYDVLSTESPMEALEIQANFDGHIDLLLTDVIMPG
                     MNGKELVQRIHVSRPTLPVIYMSGYTEGIVSARGLLKDEWLLQKPFSSASLLMEVELA
                     LDTRKPELTPA"
BASE COUNT         1081 a         2048 c         1796 g         1164 t
ORIGIN      
        1 cgggggttgc cgtctaggtc gacggtggac tgcacacctt cctgggtccc cgcatccaca
       61 ccatcgcttt cgggcgtgag gtggtagtcg tccggggcaa cgaagacggg ggcgtagccg
      121 tacgtctcgt cggtgttgat cgggaggttg ttgatgcttt cactcgtgta cttcttcccc
      181 ttgtagatca aggcggggta accgaccgag tggatgagat tgtggtcgat gcggatcagc
      241 ttcacagccc ccgccacatt cactgcgtcg ttcctgttgt cggtgaggat gctgttgaac
      301 atctggaact gggtgccacc agaatccccg ctcacgaaga tcccgcttcc attcccctcc
      361 atcaacgagt tcgcgatcgt gaaaacgccg ccagcgcagg acctcttgca gtctccttcg
      421 cccatctcca ccgcaccggt ccggttatct gccacgattg agtctcggat ctcggagttc
      481 gcgccatata gatcggcacc gtcgaatcgg ttgttggtgg ccgtcgaacg cacgatctgc
      541 gtgttcgccg cgctggatac gaaaccactc cccccgttga agctggcgtc ggaatcctcg
      601 atcagaacgt tgaggccgct ctcgatcaaa acccccgcgc tgccgggctg aacacccgat
      661 tggaacatcg agttcttgat ggtgacgtcc tcgcagggcc ccggatcgca gagaagtccg
      721 acgaaatcgt tctcctgcac cgtgactcct tcgagcgaga gcccgctcac gttctcgacc
      781 ttgatcgggg agccccccga cctgaactcg aggttctcga aagtgatgtt ctgattctcg
      841 atcccgtctt cacaggtgaa gcagaagacc ttgagtgcct cgccgttgaa gttgtcgaag
      901 atgaggttgg acaccgtcac gtcagaggtc ccggccgcga tacggatccc gtcggggaaa
      961 cgatcgttgc ctcgcagcac cggggcggcc ccctcggccc ccatgatcgt caccgactgc
     1021 ttgatcgtca ccatcggcac cttcttcgat tcgtagaaac cgtcggcgag gatcaccgtc
     1081 cctccttcgc cggctttcgc caccgccgca cgcagatccc cacccggttc gaccaagacg
     1141 gcatcggccg gtatcccgga tccggaagtt ggttcggtct ctttaacgtc atcgccatcg
     1201 cgtgtcataa aaaacgcaac gcccgccacc aggatgacgg cgccgagggc gacggccgcc
     1261 atcttcggag agatgcgtcg cgcggcagga gcgccggccg gttcgggcgg cacttcgaca
     1321 cggatctcgg gtgccttcgg ttcgaccgga gccggtggag gcgtcgccac cgcaaccggt
     1381 gctgcggcgc ctcctcccag agcagagccg agatcgtcca cgagttcggc cccggagccg
     1441 tagcgggcct cgggctcgcg cgccatcgcc tttgcaagca cggcatcgac cgccgccggc
     1501 agcgacgcgt tcttgtcggt cgccttggga cggtcgtcca tgaggtgcgc gtacataacc
     1561 gcgacgtcgg cctctttctc gaacggcacc gatccggtga gacactcgaa gagaacgcac
     1621 ccgagcgcgt agatgtcggc ctgggtaccc acggtcttac cttcgatctg ctcgggcgct
     1681 atgtaatcga tcgttccgac gaacgcgccc gtcgcagtta gaccactctt ggagccgctg
     1741 tgcttcgtca aaccgaagtc ggtgagatag atctgctcgg gggtcgtctc ggttcttccc
     1801 accagaacgt tggccggctt gacgtcacga tggatcagac cgagagcgtg cgcagcatcg
     1861 agagcgccgg ccatcgccga gatcacggtc agtgtcctct ccggggtgag cgcgccgtcg
     1921 gcttcgatga ccttcttcag gtcggttccg tcgacgtacc gcatcgcgat gaacagcgtt
     1981 ccgtcgatct cgtccgcgtc gtagaccggg atcacgttcg ggtgatcgat ggacgccgcc
     2041 atctgcgact cacgcacgaa cctggtgcgg aagttgtcat ccgccgcaag ttccggtgcc
     2101 aggatcttca acgccaccat ccggccgaga cgaaggtggc gcgccttgta cacaacgccc
     2161 atccctccac gaccgagcag ggattcgatc tcgtaaccac cgagttgggt gccgaggtgt
     2221 tggtcgctca agatttggcc gccatcttta gaaggttcgc gaacgctatc ccggaaggcg
     2281 gccggcgagc cggatccttc gcgagcgctt ggcttatcgc ccagccgaac tccttcggta
     2341 tatccgagcg ggccgcgctt gcatccggag gctcgtcgac gacatgcgcc ttgcccacct
     2401 ggaacacgcc cttgtcggcg aacggcggcc tgcccgtgat gcattcgtac gcgacacacg
     2461 ccaacgcgta gatgtcgctc gccggcccgg cctcctcccc cttgatgatc tccggcgcca
     2521 tgtaatcgag ggttcccaac acctgacccg gtcgggtgag caccgtgtaa gcccgtccct
     2581 tggcaagtcc gaggtcggta agcaccgcgc cgcgctcggc gagaacgatg ttggacggct
     2641 tcacgtcccg atgcacgatg tcgcgcccgt gaagggcatc gagagcggcc cccacctgca
     2701 gaaccacacg gacggtgccg cgcacgtcca agggcccgtc ccgttcgact atctcgttca
     2761 gaggccggcc ctccaagaac tccatcgcta tgtaaagggc cccgtccgct tccccggcat
     2821 cacggatcgc aacgagatgt tcgttgtcca cttctcgagc ggcacgcacc tcgtgctcga
     2881 accggcgacg gaacacttga tcctcggcaa gctcgggctt gaggatcttg agcgctacct
     2941 gttccccgtc ggcggacgtc gcgcggtaaa cggtcccggt ggccccgtcg ccgaggaact
     3001 cgtcgacgac gaaaccgccg atcgtctcgg tcgatgcggt tgctgccggc gattccggcg
     3061 acgtcgtcgg cacagcagac ttgtcggaca ccttcgcggc gaccttcctc gctcgcgccc
     3121 acagctccgc ggcttgatcc ggatcgagct gctcggcgcg tttcgccgct gcctcgagat
     3181 actcgagcgc cttggcccga tcgtcactcc ggtagtaata gaaagccaga agtggcaggt
     3241 gttcgtcgcg gttggcaccg tcgaggtgct cgaaaaccgc tgcgacccgc ccgtacaact
     3301 cccgccttcg ctgcggcgtg aggctcgaga atgccgcctc ttgaaccagg ccgtgcttga
     3361 acgtgcactc caaagtagga aagcgcctca cctcgcgcac gatctgggcg cgcaagagtt
     3421 cggccaggtc cgcgtctaaa tcgggaccct cggcaacctc tcgcagtacg tcgacccgga
     3481 acgtccgtcc gatcacggcg gcgatctgcg caagacgacg cgccccttcc ttcaaccgat
     3541 caatccgggc gacgagaaga ctttcgaggg ccggcggtaa caggtcttca gttctgctcg
     3601 acagcgtcca cgtccgctgt cgcacgagct ctcctccctc gatgagggcg gtcaccaatt
     3661 cctcgatgta gagcgggttg ccttcggcga gattgacgat cgtctcgcag gaagcttcgt
     3721 cgagccctac ctcgggcgca agatggtccg ccagcgcgcg cgcatcttgc gtgtccagcg
     3781 gcagcaacgg catgtcggtc atgcggtgcg ggaagtcctc caggaccgcc gcccttatcc
     3841 cacgtcccga accatcgaga tccagtcgaa gtgtcatgac gagcatcagt ggggctctgt
     3901 cggtaagtcc gagcagttga gtaagaacct cttgcgatgc cggatcgatc cagtgggcat
     3961 cgtccagggc cagcgcgacc ggcttcgatg tgcacatggc ttcgatccag gtcacatacg
     4021 cgccgccgat gcgcgcgttc aactcggctc gatccagcga atcgaataat tctttctgct
     4081 tgggatcgcc cggcaaggac agaagcttcg ccaggtaggg caacacttcc tcggctacgg
     4141 cgcccatcaa cggttgcagg tgcgcccgca gcttcgttcg caccgcgatg tccgcctcgc
     4201 cgtcccggac ccccaaccaa tccctcaccg tctgaacgaa cggccagtac gggagctcgc
     4261 cgccgtacga gagacagcgg ccctccagcc acgtggctct gtcgccgagc aggttgtgca
     4321 gctcgcccat catccgggtc ttgccgagac ccgggtcccc agagatcgca acgacctggc
     4381 ctctcccatc gacgaggtcg tcggccgcac gtttcaggat ctggatctcg ccatctcggc
     4441 cgaacagccg cccgatagat cgcgcgtgtt cggaggtgcg agcttcgatc aacctccaga
     4501 acggaaccga ctcggctttc cctttaacgg caacggcgcc tgcctcctcg aggacgaagg
     4561 ctccgtcaag cagccgagcg gtgatctcgc cgacggcgat cgacccggga tcggcggcgg
     4621 actgcaaccg tgcggcagtg ttcgtcgtat cgccgagggc cactgcctgc ggatcggatg
     4681 ctccgacgag gccgactgcg gtctgccctg aattgatccc tacgcgaacg ttgaaatcgc
     4741 tgatccccca cgccgcttcc acgtctgccg cataggtggc aacgtcttcg cggatccgca
     4801 acgcggcccg ggctgcgcgt tccggatcgt cctcgtgtgc ggtcggaacg ccgaagtatg
     4861 cacagatgcc atcgcccatg aatgcctgaa cgaaaccgcc gaactcctcg accgcggccg
     4921 ccatccgagt gacgcattca ccgatgaggg ctttgacctc gtcgggggcc agcttctcgc
     4981 ccagcgaggt cgaacccacg acgtcggcga agagggcggt gatgggtttg agctcgtcgg
     5041 aggtgcgcga tggagccggg gcctcgctcg gggtggctcc tccctcgtca ccggtcattt
     5101 cgacactttc ccgcctggtg tgacctttgt cgaccacgct ggagaacatc ccgagctcct
     5161 ggcggccgag tgccacgagg atctcgaagg cctaacgacc gcccatcaga gccgtcatca
     5221 gcagactagg ccggcgtcag ctccggcttg cgggtatcga gtgcgagctc gacctccatc
     5281 agaagggagg cggatgagaa gggtttctga aggagccact cgtccttcaa caggcccctg
     5341 gcggacacga tgccctccgt gtaacccgac atatagatga cggggagggt cggacggctc
     5401 acatggatcc gctgcaccag ctccttgcca ttcatccccg gcatgatcac gtcggtcagg
     5461 aggaggtcga tgtgaccatc gaagttcgcc tggatctcca gcgcttccat cggactctcg
     5521 gtagacaaca cgtcgtaccc ggccttcgcc aagatcctgt gcgcgacccg acgtacccca
     5581 ccttcgtcct ccaccaccag gaccgtgcat ccctcacctg atctggccgc gtcggacctc
     5641 tcctctataa ccaattcggc atccttggtt gccacgggca ggtaaagctt gaacgtcgtt
     5701 ccaacgcctg gttcggaata gacgctgatc gtgcctccca acttcttgac gaccccgtac
     5761 accgttgcga gaccgagacc cgtacccttc cctctctcct tagtcgtata gaagggttcg
     5821 aatatgcgac tccgaacctc ttccgacatc ccggttcccg tgtcggagac cgcgatgcac
     5881 acgtactccc cggcggggac ctcctctccg tcccaggaac ccccttccat gagccggaca
     5941 tgagaggtct cgatcatcaa gatgccgcca ccgggcatcg cgtcccgagc gtttacggcg
     6001 agattcaaca ggatctggtc gaattggctc ggatccatct cgattgcacg aatatcggga
     6061 gccaacgaga actggatgtc tatatcttc
//
LOCUS       DATMED010000285         6029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3675699, whole genome shotgun sequence.
ACCESSION   DATMED010000285 DATMED010000000
VERSION     DATMED010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3675699"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="VNP73_07245"
     CDS             complement(<1..1924)
                     /locus_tag="VNP73_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010058359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HWL65751.1"
                     /translation="MEHNKKDRPWIFRTYAGYATPQATNARFKSNLAKGQTGLSVAFD
                     LPTQTGYDSDHLMSKGEVGKVGVPIGHLGDMRRVFDGIPTAEMNTSMTINATAMWLLA
                     MYVSVADEQGASRADLAGTTQNDILKEYLSRGTHIFPPEQSMRLTTDLITWTLHEVPK
                     WNPINVCSYHLQEAGADPILEVAYALANAISVLDAVKATGRVSDSDFPRLVGRISFFC
                     NAGLRFVEETCKMRAFGRMWDRITKQRYGVDEDKMRRFRYGVQVNSLGLTSLQPENNV
                     YRILLEMLSVTQSKDARARAVQLPAWNEALGLPRAWDQQWSLRAQQILAYETDLLEYG
                     DIFEGSKVIEELTQKIETEAMEEFDRVLDMGGSVEALAYMKERLVAEHAGRRRAIEAG
                     DQTVVGLNRFEQTEPSHLVEGLDALAFEKIDPQAEADQMESLGAWRAERDAGAVTKAL
                     DDLKRVASTDENLFPASIEAAKAGVTTGEWADALREVFGEYRPPTGINAHVTSSDKAS
                     LTELRGKVEDAARRRGVNRLRMLVAKPGLDGHSNAAEQVALRARDGGFEVVYQGIRLT
                     PDQIARAAVEEDVHTIGLSILSGAHGLLVPEILDRLKAEGLDLDMVPVIVGGVIPEED
                     EKKLLELGVARVYTPKD"
     gene            2021..3790
                     /locus_tag="VNP73_07250"
     CDS             2021..3790
                     /locus_tag="VNP73_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HWL65752.1"
                     /translation="MTVLDIKKVGVVGCGTMGSGICEVVARAGHDVHYVEVTDEAVKA
                     GKERIERGVGRAVEKGKIERAEGDELLARISGSTDLNDLKDSDLVIEAVPEKLELKKT
                     LFEQLDGILGENAILATNTSSLPVIDMAVATKRTDRVVGFHFFNPAQVMHLVELVRTV
                     ATDDSVVESAKAFAESIGKTPVVCRDRAGFIANLLLFPYLNAAARMFDQGYASREDID
                     ASMQFGCGHPMGPLALLDLIGMDSAYEICEALWRQFRDDHDVPTPLLKQYLVAGYLGR
                     KTGSGFYRYEAPESPQIVDASAGPATGEDANIHTIGVVGTGLMASGIAEVAAKAGNTV
                     ILRGRSEASIKRSKAAVEKSLSKAVSKGKLEQSAADEILGRITTSLEFSDLAECDIVI
                     EAVAEDLALKEEIFKELDASLKPEAILASTTSSLPVVDLAAVTNRTDRVIGLHFFNPA
                     PIMKLVEVVRTVATSNEVETRSIAWIQSLRKHPVRCRDRAGFIVNFLLFPYLNDAIRM
                     HEEGYSTPEDIDTAMKLGCGHPMGPFALMDIVGLDVTLAILNSLHQEFRERRYAPAPL
                     LEHMVSAGYLGRKTGRGFYDYSQ"
     gene            3787..5115
                     /locus_tag="VNP73_07255"
     CDS             3787..5115
                     /locus_tag="VNP73_07255"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HWL65753.1"
                     /translation="MKKRLTAAALALVALIAAGTPGGARGYRERTQRIAPGVTYTSIK
                     DPRGPFRIHVVSADLSRPSTFDVELARDKLPGFETTSSMAYRNNALVAINGDYARESG
                     RPVMAFAEDGRLMQTPLVWGRNFAVNVTETDSYIGHPAISAWAYEPDVASLHSVQRMN
                     EGQPGYSEMAAYTREGGREERPPTYACATRLYPTEAPRINYEHGGVEVNHYVDETSCS
                     PSRVYPQGGTTIAAAMGSQREPEMAGFIPGEQVTLGWSLGWSGVFDTVGGNPTLVENG
                     SISVQQENDPFFTRHPRTGVGSTPDGRILMVIVDGRRPGYSVGMTPRQFAKLFLSLGA
                     DWALNLDGGGSTTMTVNGRIVNRPSDGNERAVSSALLLLPGSDPGEVQPAPAESPAPL
                     PLISPHDAAGQWRQIVRDPASTGGLASALMERGKPLPRSISRAARAFDRR"
     gene            5127..5636
                     /locus_tag="VNP73_07260"
     CDS             5127..5636
                     /locus_tag="VNP73_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL65754.1"
                     /translation="MQIQTERLLLRPLEERDFDAYLPLHSDPEVMRFVGGRHDPDAVR
                     IRFDKLKGQWAEHRLGHWATYERESGRLVGAVGLLRHDDWTATDCRVEVGWLIGKRDW
                     DRGYATEAGRASIDYAFVDLGLERLICIVDPRNVASRRVAEKLGLRLAGETIWRDYDV
                     VWYETRQHS"
     gene            complement(5624..>6029)
                     /locus_tag="VNP73_07265"
     CDS             complement(5624..>6029)
                     /locus_tag="VNP73_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65755.1"
                     /translation="AKNLWVVGSNSFRTTSTAFVARFDGREWNEVDPPPLGTAQRFRR
                     EYLGDVSVLASNDVWVTGAEVFTEKGPDADRSVLAHWDGSTWDVEYEIEGGSLDIDML
                     SPTEGWAVGTVYEESESGDVYSSDGLILHYEC"
BASE COUNT         1114 a         1883 c         1885 g         1147 t
ORIGIN      
        1 agtctttggg cgtatagacg cgggcgacac cgagctctaa caacttcttc tcgtcttctt
       61 ccgggatcac gccgcccacg atcaccggca ccatgtccag gtccaagccc tcggccttca
      121 accgatcgag gatctcggga acgaggaggc cgtgtgcccc cgacaggatc gagaggccga
      181 tcgtgtgcac gtcttcctcg acggccgcgc gcgcgatctg atccggagtg agacggatgc
      241 cttggtaaac gacttcgaac cctccgtcgc gggcgcgtaa tgcgacctgt tcggcagcgt
      301 tcgagtgtcc gtccagcccc ggctttgcga cgagcatgcg cagtcgattc accccacgac
      361 gtcgggctgc gtcctcgacc ttgccgcgca actcggtgag tgaagccttg tcggagcttg
      421 ttacgtgagc gtttatccct gtcgggggcc ggtactcgcc gaacacttca cgcaaggcgt
      481 cggcccactc gcccgtggtt accccggctt ttgccgcttc gatcgacgcg gggaagaggt
      541 tctcgtcggt cgaggcgacg cgcttcagat catcgagagc cttggtcacg gcaccggcgt
      601 ctctctcggc ccgccaggca cccaacgact ccatctgatc ggcttcggcc tggggatcga
      661 tcttctcgaa cgccaacgcg tcgagaccct ccacgaggtg cgagggttcc gtctgttcga
      721 aacggttcaa cccaactacc gtttggtccc cggcctcgat cgctcgtctg cgccccgcgt
      781 gctcggcgac gagtcgttcc ttcatgtaag ccagtgcttc gaccgagcca cccatgtcga
      841 gaacgcgatc gaactcttcc atcgcttcgg tctcgatctt ctgagtcaac tcctcgatga
      901 ccttggaacc ttcgaagatg tccccgtact cgagcagatc cgtctcgtaa gccaggatct
      961 gttgagctcg tagcgaccac tgctggtccc acgcccgggg caagccgagt gcctcgttcc
     1021 acgccggcag ctgcaccgcc ctagcccggg catccttcga ctgcgtgacg gacaacatct
     1081 cgagcaggat cctgtagacg ttgttctccg gttgcagcga cgtgagtccc aatgaattga
     1141 cttgcacgcc gtaacggaag cgacgcatct tgtcttcgtc gaccccgtac cgctgcttcg
     1201 tgatccggtc ccacatgcgt ccgaaggcac gcatcttgca cgtctcctca acgaatcgga
     1261 ggccggcatt gcagaagaac gagatgcgac ccaccaagcg cgggaagtcc gaatccgata
     1321 cgcggccggt tgccttaacg gcatcgagga cggagatggc gttggccagt gcgtacgcga
     1381 cctcgaggat gggatcggcc cccgcctctt gcaggtgata cgagcagacg ttgatcgggt
     1441 tccatttcgg aacctcgtga agcgtccacg ttatgaggtc ggtcgtcagc cgcatcgatt
     1501 gctcgggagg gaagatgtgc gtcccccggg acaggtactc cttgaggatg tcgttctgtg
     1561 tggtgcccgc cagatcggcg cgtgatgcgc cttgctcgtc ggcaaccgag acgtacatcg
     1621 ccagcaacca catcgcggtg gcgttgatcg tcatcgaggt gttcatctcg gccgtcggga
     1681 tcccgtcgaa gacccggcgc atgtctccga ggtgcccgat cgggacgccg accttgccga
     1741 cctctccctt ggacatgagg tgatccgagt cgtaaccggt ctgggtcggt aggtcgaagg
     1801 ctacggagag ccctgtttgc cccttagcga gattcgactt gaagcgcgcg ttggtggcct
     1861 gtggcgtcgc gtagcccgca taggtacgga agatccacgg gcggtccttc ttgttgtgct
     1921 ccatcgaacc cctctcgatc agcgtataga tggtacgtct gctcgatgcg actccaggga
     1981 ctgcctctaa actctttcct ttccccacac gatcggcgag gtgacggtgt tggacatcaa
     2041 gaaggttggc gtagtcggct gcggaacgat gggctccggt atctgcgagg tggttgccag
     2101 ggcgggacac gacgtccatt acgtcgaggt gaccgacgaa gcggtgaagg ccggtaagga
     2161 acgcatcgag cgcggcgtcg gccgggcggt ggagaagggg aagatcgagc gggcagaagg
     2221 ggatgagttg ctcgctcgga tctccggctc gaccgacctg aacgacctga aggattcgga
     2281 cctcgtgatc gaggccgttc ccgagaagct cgagctgaag aaaaccttgt tcgagcaatt
     2341 ggacgggatc ctcggtgaga acgcgatcct ggccacgaac acttcctcgc ttccggtcat
     2401 cgacatggcg gtggcaacga agcgcaccga tcgtgtggtc gggttccact tcttcaatcc
     2461 cgcccaagtc atgcacctcg tcgagctggt ccgaaccgtt gcgacggacg actcggtggt
     2521 tgagtcggcc aaggccttcg cagaatcgat cggaaagacc ccggtcgtgt gtcgcgatcg
     2581 ggcgggtttc atagcgaacc tgctgttgtt cccctacctg aacgcggccg cgcgcatgtt
     2641 cgatcagggc tacgcatcgc gcgaggacat cgacgcctcg atgcagttcg gttgcgggca
     2701 cccgatgggg ccgttggctc tactcgatct gatcgggatg gactctgcgt atgagatctg
     2761 cgaggccttg tggcgacagt ttcgcgacga tcatgatgtt cctacgccgc tcctcaagca
     2821 gtacctcgtc gccggctacc tcggtcgcaa gaccggatcc ggcttctacc gttacgaggc
     2881 acctgagtct cctcagatcg tggacgcgag tgcgggcccg gcaacaggcg aggatgcgaa
     2941 catccacacg ataggcgtcg tcggaacggg cctgatggca tcggggatag ccgaggtcgc
     3001 cgccaaagcc ggcaacaccg tcatcctgcg cggtaggagc gaggcttcga tcaagcggtc
     3061 gaaagctgcg gtcgagaaga gcctgtcgaa ggcggtatcc aagggcaagc tcgagcagag
     3121 cgcggcggac gagatcctgg ggcgaatcac aacctcgctc gagttctccg acctggccga
     3181 gtgcgacatc gtcatcgagg cggtggccga agacctcgca ttgaaagagg agattttcaa
     3241 agagctcgac gcctccctga agcccgaggc gatccttgcg tcaacgacat cttcgcttcc
     3301 ggttgtggat ctagccgcgg tcaccaaccg taccgaccga gtgatcggcc tccatttctt
     3361 caatccggcc cccatcatga agctggtcga agtcgtccgc accgtcgcga cctcgaacga
     3421 ggtcgaaacc aggtcgatcg cctggatcca gtcgctgcgc aagcacccgg tgcggtgccg
     3481 tgaccgggcg gggttcatcg tgaacttcct gttgttcccg tacctgaacg acgcgatccg
     3541 gatgcacgag gaggggtaca gcacccccga agacatcgac acggcgatga aactggggtg
     3601 tgggcacccg atggggccgt tcgccttgat ggacatagtc ggcctcgacg tgaccctggc
     3661 gatcctgaac tcactgcatc aggagttccg tgagcgtcgc tacgctccgg cccccttgct
     3721 cgagcacatg gtgagcgccg ggtacctggg tcgaaagacg ggacgcgggt tctacgatta
     3781 ctcccaatga agaaacgact aacggcagcg gcgcttgcac tcgtcgcgct gatcgctgcc
     3841 ggcacgccgg gtggcgcccg cgggtacaga gagcggaccc agcggatcgc gccgggcgtc
     3901 acatatacga gcatcaagga ccccaggggt ccgttccgga tccacgttgt ctcggcggac
     3961 ctgagccggc cctcgacctt cgacgtcgag cttgcgcgcg acaaactgcc ggggttcgag
     4021 acgacctcct cgatggcgta tcgaaacaac gccctcgtcg cgatcaatgg tgactacgcc
     4081 cgcgagagtg gacgaccggt catggctttc gccgaggacg gccgtttgat gcagacccca
     4141 ctcgtgtggg gacgcaactt cgctgtgaac gtgaccgaga cggactctta tataggtcat
     4201 cctgcgatct ccgcgtgggc ctacgagccc gatgtcgcgt cgctgcactc ggtgcaacgc
     4261 atgaacgagg gccaaccggg ctactccgag atggctgctt acacccgaga agggggccgc
     4321 gaggagagac ctccaacgta cgcgtgcgcg acgcggctgt atccgaccga agcgccacgg
     4381 atcaattacg aacacggtgg tgtcgaggtg aatcactacg tcgatgagac atcgtgcagc
     4441 cccagcaggg tctatccgca gggcggtaca acgatcgccg ccgcgatggg cagccagcgc
     4501 gaacccgaga tggccggatt catcccaggc gaacaagtca cgctgggttg gtcgctcggg
     4561 tggagcggcg tgttcgacac ggtcggcggg aaccccacgc tcgtggagaa cgggtcgatc
     4621 agcgtccagc aggagaacga tcccttcttc accaggcatc cgcgcaccgg ggtcggctcg
     4681 acccccgatg gacgcatcct gatggtcata gtcgatgggc ggcgccccgg ttacagcgtc
     4741 ggaatgaccc cgcgtcagtt cgcgaagctg ttcctttcct tgggggcaga ctgggctttg
     4801 aatctcgatg gtggcgggtc gacgacgatg accgttaacg gtcgcatcgt gaatcgaccc
     4861 tccgacggga acgagcgcgc ggtgtcgtcc gccttactcc ttctccccgg cagcgatccg
     4921 ggtgaggtcc agcccgcgcc ggcggagtct cccgcaccgc tccctttgat ctctcctcat
     4981 gatgcagccg gccaatggcg gcagatcgtg cgagatcccg catcgacggg gggcctcgcc
     5041 tccgcattga tggaacgagg gaaacccttg ccgcgctcta tcagtagagc cgcccgcgcc
     5101 ttcgatcgga gatagaacta gatctggtgc agatccagac cgaacgctta ctcctcagac
     5161 cgctcgaaga acgggacttc gacgcctacc tccctttgca cagcgacccc gaggtgatgc
     5221 gcttcgtggg tggccggcac gatccggacg ccgttcgcat tcgattcgac aagttgaagg
     5281 ggcagtgggc cgagcaccgg ctcggtcatt gggcgacgta cgagcgagag tcgggacgtc
     5341 tcgtcggagc ggtcggcctc ctgcggcacg acgattggac ggccaccgac tgccgagtcg
     5401 aggtggggtg gttgatcggc aaacgagact gggatcgggg ttatgcgacc gaggccggtc
     5461 gagcctcgat cgactatgcg ttcgtcgatc tcggcctgga gaggctcatc tgcatcgtcg
     5521 atccgcgcaa cgtcgcatcg agacgggttg cagaaaagct cggtttgaga ctggccggtg
     5581 aaacgatctg gcgggattac gacgtcgtct ggtacgagac gcgtcagcac tcgtaatgca
     5641 agatcaatcc gtcactcgaa tagacatctc ccgactccga ctcttcgtaa acggttccca
     5701 cggcccaacc ctccgtgggg gacagcatgt cgatatcgag tgagccgccc tcgatctcgt
     5761 attccacatc ccaggtcgat ccgtcccagt gtgcgagaac cgatcgatcc gcatccggtc
     5821 ccttctccgt aaaaacctcg gccccggtga cccacacatc gttcgaggcg agcacgctca
     5881 cgtcgccgag atactcgcgt ctgaatcgtt gagctgtgcc gagaggcggc gggtcgacct
     5941 cattccattc cctgccgtcg aagcgcgcca cgaaggctgt acttgtggtc cgaaacgagt
     6001 ttgagccgac cacccacagg ttctttgcg
//
LOCUS       DATMED010000286         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4068695, whole genome shotgun sequence.
ACCESSION   DATMED010000286 DATMED010000000
VERSION     DATMED010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4068695"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VNP73_07270"
     CDS             <1..180
                     /locus_tag="VNP73_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65756.1"
                     /translation="MLNKGKAVDGDPSLFIPHDGDQIVMRFGKEGEKIMPNPYAEQKG
                     LPQPETGPDEQPAPD"
     gene            complement(240..1262)
                     /locus_tag="VNP73_07275"
     CDS             complement(240..1262)
                     /locus_tag="VNP73_07275"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWL65757.1"
                     /translation="MSHHSHLEEIARALVAPGKGILAADESSGTIKKRFDSISLESTE
                     DNRRAYREMLFTTKGLGDHISGVILYDETIRQQASTSEPLTKILTDGGMIPGIKVDTG
                     AKALAGFEGEQVTEGLDGLRERLAEYVELGAKFTKWRAVINIGDGIPTQFCIDANAHA
                     LARFAALTQEAGMVPIVEPEILMDGDHTIERCYEATSDTLHSLFDQLYEHRVIFEGAL
                     LKTNMVLSGKDCSTQATDAEISRETLRCFYNTVPSAIPGIVFLSGGQADIPATARLNE
                     MNKLGPHPWELSFSYGRALQAPSLKAWKGEASNVEAGQHELLHRARCNGAARSGSYTD
                     QMETAA"
     gene            complement(1514..1693)
                     /locus_tag="VNP73_07280"
     CDS             complement(1514..1693)
                     /locus_tag="VNP73_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWL65758.1"
                     /translation="MFGKIGGGEILLILLIVLLLFGAARLPRLARSLREARDEFQKGS
                     DEDAAEKKEQEQEKA"
     gene            1753..>2549
                     /locus_tag="VNP73_07285"
     CDS             1753..>2549
                     /locus_tag="VNP73_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWL65759.1"
                     /translation="MTSTPISELAVRDALHTVIDPEIGKPIDEIGMLGGFEIDGSAVR
                     VDVLLTVSNCPLQDELVRRVNDALHVVDGVGSVDVRMGVMEEEKRAELAANLQKEIQF
                     RDPNSRTTVIGISSGKGGVGKSTVTTNLAAAMQARGHVVGVLDADIWGFSVPRMLGLR
                     GERPVAFDGTIMPLNRNGVKVISIGFFLPNEEEPVVWRGPMLHKALEQFLSDVYWGAD
                     LEYLLIDLPPGTGDISLSLATFLPNMGMVVVTTPQPVAQKVAERAAN"
BASE COUNT          450 a          793 c          800 g          506 t
ORIGIN      
        1 atgctcaaca agggcaaggc agtggacggc gacccgtcgt tgttcatccc gcacgacggc
       61 gaccagatag tcatgcgctt cggaaaagag ggcgagaaga tcatgcccaa cccgtacgcg
      121 gagcagaagg ggctgccgca gccggagacc ggaccggacg aacagcccgc gccggactaa
      181 gccgctgctg ctggacctgc gtctagatca ggccgcttct tccatctgcc cggccgcgtc
      241 tacgcggcgg tttccatctg atcggtgtag ctacccgacc gcgctgcgcc gttgcagcgt
      301 gcgcgatgta gaagctcgtg ctgcccggcc tcgacgttcg aggcttcgcc cttccacgct
      361 ttgagagaag gagcctgcag ggcgcggccg taagagaagg acaactccca cggatggggg
      421 ccgagcttgt tcatctcatt gagtctcgcc gtagccggta tatcggcctg gccgccggag
      481 aggaaaacga ttccggggat cgcgctgggc acggtgttgt agaagcagcg cagcgtctcg
      541 cgcgagatct cggcgtcggt ggcctgggtg gagcagtcct tgcccgacaa aaccatgttc
      601 gttttgagca acgcaccttc gaagatcaca cggtgctcgt agagctgatc gaacagcgaa
      661 tgaagtgtgt cggacgtggc ttcgtagcaa cgctcgatcg tgtggtctcc gtccatcaag
      721 atctcgggct ccactatcgg aaccatcccc gcctcttgcg ttagcgcagc gaagcgagcc
      781 agcgcatgag cgttggcgtc gatgcagaac tgggttggga tcccgtcgcc gatgttgatc
      841 acagcacgcc acttcgtgaa cttggcgccg agctcgacgt actcggccag cctctcgcgc
      901 aggccgtcga gtccttcggt tacctgctcg ccctcgaaac cggccaacgc cttggctccc
      961 gtgtcgacct tgatgcccgg gatcatcccg ccgtcggtga ggatcttcgt cagcggctcg
     1021 ctcgtcgatg cctgctgccg gatcgtctcg tcgtaaagga tgacgccgga gatgtggtcg
     1081 cccaaccctt tggtcgtgaa caacatctcg cggtatgcgc ggcggttgtc ttccgtggac
     1141 tcgagcgaga tcgaatcgaa gcgcttcttt atcgttccgg aactctcgtc cgccgcgagg
     1201 atccccttgc cgggtgcaac gagtgcccga gcgatctcct cgagatgtga gtggtgcgac
     1261 atcgaacgcc tcctcctatc gcggtaaccg tgtcacttca cgcacatgat tatcacgcgt
     1321 tgcttaatcg cagctcaggg ggcattgacc cttgaacgat ccgggtctat gttcgaactt
     1381 acctgggcgg ttcatcgggg tgatgtcttt ccccggtccc ggacggaggt tcggcagtgc
     1441 ccactagggc acgtgacgct tggagcctcg tctagcaggg gccggcgggg aggaagcctt
     1501 cccgaagggc cgcctaggcc ttttcttgct cctgttcctt cttctcagcg gcgtcttcgt
     1561 ccgatccctt ttggaactcg tcgcgggcct cacgcaggct gcgggcgagt ctcgggagcc
     1621 gggcggcgcc gaagagcaag aggacgatca ggaggatgag gaggatctct ccaccaccga
     1681 tctttccgaa cataacgaca cgatactcct tgcgctactt tggaggccct cgttggtttt
     1741 agaaggactc cgatgacctc cacgcccatc tcagagctgg ccgttcgcga cgcgcttcac
     1801 accgtcatag accccgagat cggcaagccg atcgatgaga tcggcatgct cggcggcttc
     1861 gagatcgacg gctcagccgt gcgggtggac gttctcttga ccgtctcgaa ctgcccgcta
     1921 caggacgagc tcgtgcggcg ggttaacgat gctcttcacg tcgtggacgg cgtcggttcg
     1981 gtcgacgtgc ggatgggcgt gatggaggag gagaagaggg ccgagcttgc ggccaatctc
     2041 cagaaggaga tccagttccg cgacccgaac tcgcgcacaa ccgtcatcgg catctcctcg
     2101 ggcaagggtg gcgtcggcaa gtcgacggtc acaaccaacc tcgccgcggc catgcaggcc
     2161 cgcgggcacg tggtcggcgt cctggacgcc gacatctggg gtttctccgt gccgcggatg
     2221 ctcggacttc gcggcgagcg tcctgtggct ttcgacggga cgatcatgcc cctcaaccga
     2281 aacggcgtga aggtcatatc gatcgggttt ttccttccga atgaagaaga gcccgtcgtg
     2341 tggcgggggc cgatgctgca taaggcactg gaacagttcc tgtccgacgt ttattggggc
     2401 gcagacctcg agtacctgtt gatcgacctc cctccgggca ccggcgacat ctcactgtcg
     2461 ctcgcgacgt tccttccgaa catgggcatg gtcgtagtaa ccacgccgca gcccgtcgcc
     2521 cagaaggtcg ccgaacgcgc cgccaacat
//
LOCUS       DATMED010000287         3654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_604829, whole genome shotgun sequence.
ACCESSION   DATMED010000287 DATMED010000000
VERSION     DATMED010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604829"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VNP73_07290"
     CDS             complement(<1..741)
                     /locus_tag="VNP73_07290"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL65760.1"
                     /translation="MSTLVTGATGFIGSHMVEVLAEAGHDVIAADLPSALDAPADDRT
                     RWPEVCAQAGAKLVPLDLTDPASVESAIAGTELVFHIAAVFDYTAPESVLRSVNVEGT
                     RNLFDGLVARGECRRVVNWGAGGIYGPPVPSEGEFTEDSPKRPTNPYLVSKWDQEKLA
                     HRYRDKGIEVTSVRTTSPYGPRAVYGSGQLLVQMAKSPVAFSNLTGNIPFVHVRDLCR
                     AALHLASYEAADGEAYNVTDDGRIDAIAL"
     gene            765..2078
                     /locus_tag="VNP73_07295"
     CDS             765..2078
                     /locus_tag="VNP73_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWL65761.1"
                     /translation="MDVVPLLQELLRFDTTNPPGNELPAAEYLHSRLTDVGLKSEILT
                     SAGGRANVVARTEGPGDKPAIVLLSHLDVVGVERDRWRQDPFGGEIHGGELWGRGALD
                     MKGIAAMHTRAVSDLVTSDRDPRREVILVSVADEEAGGTEGARWLLAEHADKVGFADG
                     RPPPEVLGEGAFGLSEVFARPVMPIVLGEKSALWLNLNARGDPGHGALPPQNQAALNL
                     TRAVTAVAGYSTPRIHPVMREQFAVFAREAPQPLATVFKMLSSPAAGGIARATKRALA
                     ARPAVAALLSDTTATTQLSGGYKHNVVPGEAHAALDCRLLPDTDVDGFVTAMRKRVKR
                     LSVEVEEVSRHSGPVSRKGPLFEALVRASRDAEPEAVVVPSLTAGITDVRFFRSRGAT
                     GYGWVPLVLSAADLATIHGHDERVGVQALEKAATSMSRVVLEAAT"
     gene            complement(2075..2212)
                     /locus_tag="VNP73_07300"
     CDS             complement(2075..2212)
                     /locus_tag="VNP73_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65762.1"
                     /translation="MSDPEPNSKRPWKLRLAELLLWVAVAVGTAVIMISMSERLLPAN
                     F"
     gene            complement(2209..2841)
                     /locus_tag="VNP73_07305"
     CDS             complement(2209..2841)
                     /locus_tag="VNP73_07305"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWL65763.1"
                     /translation="MLISVGMGVLMFVGWTLWGTGFYYSDQQNDLRTEFDRIEVAVPV
                     SDGPSGPPRGYAPRAGKPVFNLVIPAIKLDQIVVEGVGTEELRKGPGHYPECRPGFSK
                     PLCTEFDEAWPGEKGRVIVSGHRTTYGAPFWDLDKLKRGDEVITKTKWGRFTYTVTKK
                     SIVRPDSLAIAVPSNNREIVLTTCNPRFSASQRLVVFAEMKEALAMSDRT"
     gene            2916..3158
                     /locus_tag="VNP73_07310"
     CDS             2916..3158
                     /locus_tag="VNP73_07310"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HWL65764.1"
                     /translation="MPKSRSKRVRRQPPPKAKPKSSPPWVGGLFFLLLVGGVVVIICH
                     YLGVLPWPSPGGTQPIQLWVGLGMITASFVLATQWH"
     gene            3221..3544
                     /locus_tag="VNP73_07315"
     CDS             3221..3544
                     /locus_tag="VNP73_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65765.1"
                     /translation="MVKALLALVAIAAIAVLGEAYGYLRSGVAVIVFFALASFGFKYW
                     RDVGLIPPEPEQEDVRDQQLRYVCRVCGLQLRVEISARDRAPTHCAEPMELVKEEPRT
                     PLRPV"
     gene            complement(3541..>3654)
                     /locus_tag="VNP73_07320"
     CDS             complement(3541..>3654)
                     /locus_tag="VNP73_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65766.1"
                     /translation="LMTTLVHALEERGGRYGLQTMCEGGGLANATIIERLS"
BASE COUNT          666 a         1141 c         1159 g          688 t
ORIGIN      
        1 cagtgcgatc gcgtcgatgc gtccgtcgtc ggtgacgttg tacgcctcac catcggccgc
       61 ttcgtaggag gccagatgga gggccgcgcg gcacaggtct ctcacgtgca cgaaggggat
      121 gttgccggtg agattgctga acgccaccgg actcttagcc atctgaacga gcagttgccc
      181 ggaaccgtag acggcgcgag gaccgtatgg agaggtagtt ctgacggaag ttacttcgat
      241 gcccttgtcg cgatatcggt gcgcgagttt ctcttggtcc catttcgaaa ccagatacgg
      301 gttggtcggc cgcttgggcg agtcctcggt gaactcaccc tcgctcggaa ccggtgggcc
      361 gtagatgcca ccggcgcccc agttcacgac tcttctgcac tctccccgag cgacgagacc
      421 atcgaacagg ttgcgtgtgc cctcgacatt caccgagcgc agaaccgatt ccggagccgt
      481 gtagtcgaac actgcggcga tgtggaacac cagctcggta cccgcgatcg ccgactcgac
      541 tgacgccggg tcggtgagat cgagtgggac gagcttggct ccggcctgcg cgcatacctc
      601 cggccagcgg gtccggtcgt ctgccggtgc atccaaagct gaggggaggt cggcagcgat
      661 cacgtcgtgg cctgcttcgg cgagaacctc gaccatgtgc gaaccgatga atccggttgc
      721 tccggtgaca agtgtcgaca cgagccggag agtagcctct gaaaatggac gttgtccccc
      781 tgctgcagga actactccgg ttcgacacga cgaacccacc gggcaacgaa ctaccggccg
      841 ccgagtacct ccactcgcgt ctcaccgatg tcggtctcaa gtccgagatc ctgacgtcag
      901 ccggggggcg agcgaacgtc gtcgcgcgca cagagggccc gggcgacaag ccggcgatcg
      961 tccttctctc ccacctggat gtcgtagggg tcgagcgcga ccggtggcga caggacccct
     1021 tcggtggcga gatccacgga ggcgaactgt ggggtcgcgg agcgttggac atgaaaggca
     1081 tcgcggcgat gcatacccgt gcagtgagcg atctcgtcac gagcgatcgc gatcctcggc
     1141 gtgaggtgat cctggtctcc gtcgcagacg aggaggccgg gggcaccgaa ggagcacgtt
     1201 ggttactcgc agaacacgcc gacaaggtgg gattcgccga cggccggcct cctccggagg
     1261 tcttgggaga aggggcgttc ggcctgtccg aagttttcgc ccggcccgtc atgccgatcg
     1321 tccttggcga gaagtcggcg ctgtggttga acctgaacgc tcgcggcgat ccgggacacg
     1381 gggccctccc acctcagaac caagcggctc tcaatcttac ccgcgcggta acggcggtag
     1441 ccggttattc gacccctcgc atccatcccg tgatgcggga gcagttcgcg gtcttcgcac
     1501 gagaagcccc gcaacctctg gccacggtgt tcaagatgtt gtcttcgccc gcggccggcg
     1561 ggatcgcccg cgcgaccaaa agagcactgg cggcacgccc tgccgttgcg gcgctcttgt
     1621 cggatacgac cgcaacgact caactgagtg gcggttacaa gcacaatgtc gttcccggcg
     1681 aagcgcacgc ggcgctcgac tgccgtctgt tgccggacac cgacgtcgac ggattcgtaa
     1741 ccgctatgcg aaagagagtg aagcgtctct ccgttgaagt ggaagaggtg tcgcggcact
     1801 ccggtccggt gagccgaaag gggccgctct tcgaagcgct ggtgcgagca tcacgcgacg
     1861 ccgagcccga agcggtcgtg gttccctcct tgacggccgg cataacggac gtgcgcttct
     1921 tcaggtcacg cggcgcgacc ggatacggat gggtgccgct cgtgttgagc gcagccgacc
     1981 tggccacgat ccatggacac gacgaaaggg tgggcgtgca agctctggag aaggccgcca
     2041 cctcgatgtc gcgagtcgtt ctggaagcgg ctacctagaa gttagcgggc aggagtctct
     2101 ccgacataga gatcatgatc acggccgtac cgactgcaac ggccacccag agaaggagtt
     2161 cggccaatcg gagtttccac ggacgcttcg agttcggctc tggatcactc atgtgcgatc
     2221 gctcatcgcg agcgcctcct tcatctcggc gaacacgacc aaccgttgcg aggctgagaa
     2281 gcgaggatta caggtagtca acacgatctc ccggttgttg ctgggtacgg cgatcgcaag
     2341 tgagtcgggc cgaacgatcg acttcttggt gacggtgtag gtgaagcgcc cccacttcgt
     2401 cttcgtgatc acctcgtcgc cccgtttcag cttgtcgaga tcccagaacg gggcgccgta
     2461 agtggtgcgg tgaccggaca cgatcacccg gcctttctct cccggccacg cttcgtcgaa
     2521 ctccgtacaa agaggtttcg agaaaccggg gcggcactcc ggatagtggc cgggtccctt
     2581 gcggagctct tcggtcccga caccttccac gacgatctga tccaacttga tggccgggat
     2641 caccaagtta aatacgggtt tgccggcacg cggcgcgtaa cccctcggcg gccccgaggg
     2701 tccatccgac accggcaccg cgacctcgat ccgatcgaac tcggttcgaa ggtcgttctg
     2761 ttggtcgctg tagtagaagc cggtgcccca cagcgtccat ccgacgaaca tgagcacgcc
     2821 catgccgacg gagatcagca actttcccag tattcgaagt atctgcatct gctcccatct
     2881 gattatcggg tgccgcggct actatctgga gccttatgcc gaagagccgt tcgaaacgcg
     2941 tacgtagaca accacctccc aaggccaagc cgaagtcctc acccccatgg gtgggtggcc
     3001 tcttcttcct gcttctcgtc gggggggtcg tggtgatcat ctgccactac ctgggggtcc
     3061 tgccgtggcc cagcccggga gggacacaac cgatccaact atgggtcggg ctgggcatga
     3121 tcacggcctc gttcgtgctc gccacccagt ggcactaggg gcgttaaatc acaaggatgt
     3181 tctttatcca ccgctgtgga gagacctgtg gagaactccc gtggtgaaag ccctgctcgc
     3241 tctggtcgcg atcgccgcga tcgcggtcct gggtgaggcc tacgggtatc tgcggtcggg
     3301 cgtcgccgtg atcgtcttct tcgcactcgc atcgttcggc ttcaagtact ggcgagatgt
     3361 cgggttgatc ccacccgagc ccgagcagga ggacgtccgc gaccagcagc tccgttatgt
     3421 ctgccgggtg tgtggattgc agctgagggt cgagatctct gcacgcgacc gggcaccaac
     3481 ccactgtgcc gaacctatgg aactggtcaa agaggagcct aggactccac ttagaccggt
     3541 ttagctgagt cgctcgatga tcgtcgcgtt cgcgagcccg ccgccttcgc acatcgtctg
     3601 gagaccgtag cggccgccac gctcttcgag tgcgtggacc aaagtcgtca tcaa
//
LOCUS       DATMED010000288         9713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2541539, whole genome shotgun sequence.
ACCESSION   DATMED010000288 DATMED010000000
VERSION     DATMED010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541539"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1080)
                     /gene="ruvB"
                     /locus_tag="VNP73_07325"
     CDS             complement(55..1080)
                     /gene="ruvB"
                     /locus_tag="VNP73_07325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWL65767.1"
                     /translation="MSDEILATAVDPVEQEAELSLRPRSLEEFVGQPELKEHLAIVLE
                     AALARNEPAGHLLLSGPPGLGKTSLAHVVANEMGANIRPTSGPALERAGDLAAILTNL
                     ADGDVLFIDEIHRLPRIVEEVMYPAMEDFQLDLVIGKGPSAQTIRLDLPRFTLVGATT
                     RAGLITGPLRSRFELAERLDYYPALDLEQIVERSGRILEVHIDATGISEIAKRSRGTP
                     RIANRLLRRVRDFAQVRAAGSIDGTVARDALEVFKVDQEGLDKVDVAILEALIGRFGG
                     GPVGLSTLAIAVGEEPDTIEDVYEPFLLRLGFLRRTPRGRTATEAAFRHLGSEVPIGD
                     AAARLFS"
     gene            complement(1080..1658)
                     /gene="ruvA"
                     /locus_tag="VNP73_07330"
     CDS             complement(1080..1658)
                     /gene="ruvA"
                     /locus_tag="VNP73_07330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWL65768.1"
                     /translation="MIGFVRGKVAARTEESCLVEVAGIGYRLACSSTTLAKLPADGEA
                     IQLWTHVHVREDVLALYGFTSEAEQKMFEALLGVGGVGPKVALQVCSAFSPEQFRKAL
                     VTDDVAGFAAVPGIGKKTAQRIVLDLKDKLDLPDLSLVGARPDALSQARSALENLGYS
                     AGEVRVALGEVAAEEEASVENVVKSALKVLAN"
     gene            complement(1655..2155)
                     /gene="ruvC"
                     /locus_tag="VNP73_07335"
     CDS             complement(1655..2155)
                     /gene="ruvC"
                     /locus_tag="VNP73_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWL65769.1"
                     /translation="MRVLGIDPGLTTTGFAVVERKAGGMQAAGVGAIVTPKALPTAAR
                     LSFLRAQLLEIIAQHRPEVMAIERLFFNVNVRTAMAVGQASGVALEASASSGLEVHHY
                     TPLEVKQSVVGVGNASKSQVQSMVAALLGLEAQPKPADAADACALCICHINRSGLARA
                     LEAALR"
     gene            complement(2242..3075)
                     /locus_tag="VNP73_07340"
     CDS             complement(2242..3075)
                     /locus_tag="VNP73_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65770.1"
                     /translation="MAIRVRTSIRRRPVRVLALVGLLLLLLAPPGDAGGRKLPKHTSS
                     RYVSAGYNYVSGSSDGGPEYNVASPMEFVVERNERWASITIDDRTGLPVAAVASQDVD
                     GDGSPDKTWDVCGATVEPFEIAPGKPLVVAPAPVPCSGGAASTTGAVFVEFISPGAAP
                     KGPVVQRVEPEQQVTYAGLIYQGADGGVASGSYGMDRWVTDRYVSVAVTDATGQAAFF
                     TIYPQNAPPIAVCGGTEEPIEIPAGGDELYVEMGTGICPDGTPATSTHGVIDITLSNL
                     P"
     gene            complement(3183..4457)
                     /locus_tag="VNP73_07345"
     CDS             complement(3183..4457)
                     /locus_tag="VNP73_07345"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWL65771.1"
                     /translation="MTEVVRPPLEDPRLRQVADQVDRARFGGMLLDSDWNLVWVSEEM
                     IKFLFEPPYEPLLGQHVIHNYMSDAFCRLISEESQIEMMMKMAPYLIWGTPGGKEGLK
                     KIMSEAHQHYFELGRDVPQWAKDAGINHEQTLEMLLEGLDTVEPVEPPGMWIETFEFL
                     YKDLPPTRIAEMQMPLRDDSGDIFGYLVLYMPALPAGVLQLVARGDEGMFERMIDLVE
                     PGRNPAAILFCDLQDSGVLSRRLPSAAYFKLVRALTDAIDECVVQHKGIVGKHAGDGV
                     TAFFLADDLGSPSGAAHAAIEAGRRIAAGAGRVAKEVGEETGLIEAADCVVNVGLHWG
                     GTLYMGQLVTGGRLEVTALGDTVNECARIQETARDGTILGSKQLIENLTPEDAETLNV
                     DPDSLVYRTVAELSSATEKAKRDAGGIPVTTL"
     gene            complement(4454..5203)
                     /locus_tag="VNP73_07350"
     CDS             complement(4454..5203)
                     /locus_tag="VNP73_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWL65772.1"
                     /translation="MSGHSKWSTIKRKKGAADAARSKLWAKLLRAVEVAAREGGGSLD
                     ANPTLASAVEKAKGSSVTNDNIERAIKRGTGELEGETYEEISYEGYGAGGVAVLVSCL
                     TGNRNRTAQDVRSLFTKHAGKMAEPGAVSWMFEPKGVVIVLKDGVDEDEVFMIAADAG
                     AEDVRGSEDSIEVITPTDALKPVRDALEGAGVSIESYELTQVPKTTVPLEVSDAKKVL
                     TLIDSLEELDDVQDVYANFDISDDVLESVAS"
     gene            complement(5207..5776)
                     /gene="pdxT"
                     /locus_tag="VNP73_07355"
     CDS             complement(5207..5776)
                     /gene="pdxT"
                     /locus_tag="VNP73_07355"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWL65773.1"
                     /translation="MKVGVLALQGDVREHVRAVEAAGGTPLEVKHEAQLESVDALIVP
                     GGESTTIGKLLDRFGLLQPIADRAKAGMPLYGTCAGAILMATEIDGAQDAPHRFGLLD
                     MAIERNAYGRQIDSFETDLVVRGVAEPFRAVFIRAPVIRRVGDDVEVLAELEGRPVLV
                     RRGNLLASTFHPEMTDDYRVHEMFLSMVG"
     gene            complement(5782..6678)
                     /gene="pdxS"
                     /locus_tag="VNP73_07360"
     CDS             complement(5782..6678)
                     /gene="pdxS"
                     /locus_tag="VNP73_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWL65774.1"
                     /translation="MADISRDTGGARVKRGLAEMLKGGVIMDVTTAEQAKIAEEAGAV
                     AVMALERVPADIRAEGGVVRMADPEKISEIMDTVSIPVMAKCRIGHFTEAQVLQSLGV
                     DYIDESEVLTPADESNHVNKWDFDVPFVCGATNLGEALRRLGEGAAMIRSKGEAGTGN
                     VVEAVRHMRSITSGIRKLATLGGEELMAEAKELRAPFDLVREISETGTFPVVLFTAGG
                     IATPADAALMMHLGADGVFVGSGIFKSGDPATRARAIVEATTYFKEPEIVAKVSRGLG
                     EAMVGINLDDISDEQRLARRGW"
     gene            complement(6735..7757)
                     /locus_tag="VNP73_07365"
     CDS             complement(6735..7757)
                     /locus_tag="VNP73_07365"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HWL65775.1"
                     /translation="MRIKRSLVVAMLFGLVFSAACSEEEPTVASTPTPTPTPDPVCPL
                     TGEDIPSGVDIRKPAVAVKVENSSAARPQSGLEDADVVYEEIVEGGITRFMAIYHCKN
                     SKKVGPVRSARFDDPKIAKPYTRILAFSGGNSIVERELVRQNLIALQENDGVGALYRV
                     PPGVLEIHNLFGDTKKIRKLPPAKKADGPKKDLFEFGDLPDGLKKARHVAIHFTASNE
                     IEYRWKKGAWHRYEAGAPFMSETNGQIAVPNVLVQEVMVNNSPKIVDSAGNPSPDIQL
                     VGKGRALLFRDGKVMKGTWEIEDEGRFPILEKRNGDEFVFAPGPVWIELVPSHKGQVK
                     GSFSFK"
     gene            complement(7764..8807)
                     /locus_tag="VNP73_07370"
     CDS             complement(7764..8807)
                     /locus_tag="VNP73_07370"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HWL65776.1"
                     /translation="MDKRVKVALGAVGGIVVLSAGAFFLLGNRPSDIPIIKEITAPAT
                     CPLSGSEPAKEAALDRPAVAVKIENAAIAYPLSGLEDAELVYEELVEGGVTRFMAVYH
                     CTDSTKAGPVRSARVVDPAIMGAITRILAYSGQNAPVLRALEEAEVVRLDETEAGEGL
                     QRIERSGISMEHTLYADTKILRKLGTKEFDDPPPGDTFTFGDIEGKTKPAKQIEINFN
                     PVNTIRYDWSDDGWLRFEADEPFMAESGDQIIVNNVLIEEHEVIFSNTITDVAGNPSL
                     EIANETGSGRAFLFRDGQVIKGKWSREDVGDAVTFETKEGDEFVFAPGSIWIELLPSK
                     KGEVKGSFDFKKK"
     gene            complement(8957..>9713)
                     /locus_tag="VNP73_07375"
     CDS             complement(8957..>9713)
                     /locus_tag="VNP73_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65777.1"
                     /translation="SQRSSDLAEVGPGLLMATAARDLPDWGGDPPTYPLGKRIWLFVV
                     LAGPLVLVSAALSFPFGGGWVGFALTLTIVSMAMGVYVGRLRKQILKRFDAVPLDESG
                     ARFRNLVEGLAEGLESPRPEIFLVTGDKPNAIVLRGHPPVIGVTRSLLDTYSRTEQEA
                     VAAHCLVRIHTGHLFFSELAARLGRSASRFAPKVGSEDDLQTAAITRYPPGLARAIQH
                     AGPVQGPTACLWFVAHSPSHYPVVSRIDALGDL"
BASE COUNT         1704 a         3223 c         2952 g         1834 t
ORIGIN      
        1 agagccgcca gagcggctct cggagaccca tgctactaga cctttcaggg ggcctcagga
       61 gaagaggcga gccgccgcgt cgcctatcgg gacctcgctg cccaggtgac ggaaggccgc
      121 ctcagtagcc gttcggcccc gcggcgtacg gcgcaagaac ccgagccgga ggaggaacgg
      181 ctcgtacaca tcctcgatcg tgtccggctc ctcacccacg gcgatcgcaa gggtggagag
      241 cccgaccggg ccacctccga aacgaccgat gagggcttcg aggatcgcga catcgacctt
      301 gtcgagcccc tcctggtcga ccttgaagac ctcgagcgcg tcccgagcca ccgtgccgtc
      361 gatgctgccc gcggcccgca cctgagcgaa atcccgcacc ctgcgcagta ggcggttcgc
      421 gatccgtggg gtcccgcggg accgtttggc gatctcggag atgccggtcg cgtcgatatg
      481 aacttccagg atccggccgg agcgctcgac gatctgttcg aggtcgagag ccgggtagta
      541 gtcgagtcgt tcggcgagct cgaagcggct gcgaaggggg ccggtgatga gcccggccct
      601 cgtcgtcgcc cccaccagcg tgaagcgcgg caggtcgaga cggatcgtct gtgcacttgg
      661 ccccttgccg atcacgaggt cgagttggaa gtcctccatc gccggataca tcacctcttc
      721 cacgatgcgc ggcaagcggt ggatctcgtc gatgaacaga acgtcgccgt cggcgaggtt
      781 cgtgaggatc gccgccaggt cgcccgctcg ttcgagcgcg gggcccgacg tcggacggat
      841 gttcgcgccc atctcgttcg caacgacgtg cgccaggctt gtcttgccca acccgggggg
      901 gccggagaga agaaggtgcc cggccggttc gttacgcgcg agcgcagcct cgagaacgat
      961 cgcgaggtgc tccttcagtt cgggctggcc gacgaactcc tccagcgaac gaggtctgag
     1021 ggaaagctcg gcctcctgct cgacgggatc gaccgcggtc gccaggatct catccgacat
     1081 cagttggcca gaaccttgag cgcggatttc acgacgtttt cgaccgaggc ttcctcctct
     1141 gcagcaactt ctccaagagc gacacgcacc tcgccggcgg agtatccgag gttctcgagc
     1201 gcggagcgcg cttgcgacag cgcgtcgggt cgcgctccga ccaaactgag atcgggaaga
     1261 tcgagcttgt ccttgagatc gagaacgatg cgctgcgcgg tcttctttcc gatgccggga
     1321 acggccgcga agccggcgac gtcgtccgta acgagagcct tacggaactg ctccggcgag
     1381 aacgccgaac agacctggag cgcgaccttg ggcccgacac cgccgacacc gagcagcgcc
     1441 tcgaacatct tctgctcggc ttcgctggtg aaaccgtaga gagccagcac gtcctcgcgc
     1501 acgtgcacgt gcgtccagag ttggatcgcc tcgccgtcgg cgggaagttt cgcaagagtc
     1561 gtcgaggaac aagcgagcct gtagccgatc ccggccacct ccacgagaca ggactcctcc
     1621 gtccgagctg caaccttgcc gcgcacgaac ccgatcatct gagcgccgcc tccagagcgc
     1681 gggccagccc gctgcgattg atgtggcaga tgcacaaggc gcacgcgtcg gccgcgtcgg
     1741 ccggcttggg ctgggcctcg agaccaagaa gcgccgcaac catcgactgc acctggctct
     1801 tggaggcgtt gcccacaccg acgacggatt gcttcacctc tagtggcgtg tagtgatgga
     1861 cctcgagtcc actggaggcc gaggcttcga gggctacacc cgacgcctgc ccgacggcca
     1921 tcgccgtacg cacgttgacg ttgaagaaga gccgttctat cgccatgacc tcggggcggt
     1981 gctgggcgat gatctcgagt agctgggccc ggaggaaaga gagccgggcc gcggtgggca
     2041 gcgccttggg cgtgacgatg gcgcccacgc cggcggcctg catcccaccg gccttacgtt
     2101 ctacgactgc gaacccggtc gtagtcagac ccgggtcgat ccccaagacc ctcacagctg
     2161 ctccttgata gcgaacactt gttcgctaga agcctaaggg agccctatga cgaaaacccg
     2221 gtttttggcc cgatccgggg cctaggggag gttggagagc gtgatgtcga tcacgccgtg
     2281 cgtcgatgta gccggggtgc catccgggca gatcccggtg cccatctcga cgtaaagctc
     2341 gtcaccaccg gcggggatct cgatcggttc ctcggtccct ccgcaaaccg ctatgggtgg
     2401 agcgttctgc gggtagatcg tgaagaacgc agcctgcccg gttgcgtccg tgacggccac
     2461 actcacatac ctatccgtga cccatcgatc catcccgtat gaccccgaag cgacccctcc
     2521 gtcggctccc tggtagatca gacccgcata ggtgacttgc tgttctggtt cgacgcgctg
     2581 tacgaccggc cctttcggcg ccgcacccgg cgagatgaac tcgacgaaaa ctgctcccgt
     2641 ggtcgaagct gcgcccccgg aacaaggaac gggggccggc gccaccacca ggggcttgcc
     2701 cggagcgatc tcgaagggct cgaccgtcgc gccgcatacg tcccatgtct tgtcgggaga
     2761 gccgtctccg tcgacgtcct gactggccac cgcggccaca gggagacccg tcctatcgtc
     2821 gatcgtgatc gatgcccacc gctcgttgcg ctcgacgacg aattccatcg gtgaggccac
     2881 gttgtattcg ggacccccat cgctggagcc ggagacgtag ttgtagcccg cggacacata
     2941 tctgctgctg gtgtgcttcg ggagcttcct tccgccggca tcgcccggag gggccagaag
     3001 caggaggagc agcccaacga gcgccagcac gcggaccggc ctgcgtcgga tcgaggttcg
     3061 gactcggata gccatacaag caagattcgt tccctgcact gccaatcctg cggcacgatc
     3121 gtgccgcccg ctccgttggg cagcgggtgg gtggtaccgg atgggcggcg aagcccgctc
     3181 ctctagagag tcgttactgg gatgccgcct gcgtcacgct tggccttctc ggtcgcgctg
     3241 ctcagctcgg caaccgtccg gtacaccaac gagtccgggt cgacattcag cgtctcagcg
     3301 tcctccggtg tcaggttctc gatcagctgc ttcgacccca ggatggttcc gtcgcgtgcg
     3361 gtctcttgga tgcgcgcgca ttcgttcacg gtgtctccga gggcggtcac ttccagacga
     3421 cccccagtca ccagttgccc catgtagagg gtcccgcccc agtggagccc cacgttgacc
     3481 acacaatccg cggcttcgat caatccagtc tcttctccga cctctttggc tacgcggccc
     3541 gcccctgcgg cgatacgtcg accggcctcg atcgcagcat gcgcggcccc gctcggcgat
     3601 ccgaggtcat ccgcaaggaa gaaagccgtc acgccgtcgc ctgcatgctt gccgacgatg
     3661 cccttgtgct gaacgacgca ttcgtcgatc gcatcggtca gggctcgtac cagtttgaaa
     3721 tacgccgcgc tcgggagcct gcgtgacagg actccagaat cttgcaagtc gcagaagagg
     3781 atcgccgcgg ggttgcggcc cggctcgacc agatcgatca tgcgctcgaa catgccttcg
     3841 tcaccccgag cgaccaactg caggacaccc gccggtagcg cgggcatgta cagcactaag
     3901 taaccgaata tgtcacccga gtcatcgcgc aacggcatct gcatctccgc gatgcgggtc
     3961 gggggcaggt ctttgtagag gaactcgaac gtttctatcc acatgccggg gggctcgacc
     4021 ggttcgacgg tgtcgagtcc ttcgaggagc atctcgagcg tctgttcgtg gttgatgccg
     4081 gcgtccttcg cccattgagg tacgtcgcga ccgagttcga agtagtgctg atgggcctcg
     4141 gacatgatct tcttgaggcc ctccttgccg ccgggcgtcc cccagattag ataaggcgcc
     4201 atcttcatca tcatctcgat ctgactctct tcgctgatga gacggcagaa cgcatcgctc
     4261 atgtagttgt ggatcacgtg ttgcccgagc agaggttcgt aaggtggctc gaagaggaac
     4321 ttgatcatct cctcggacac ccacacgagg ttccaatccg agtccagcag catcccgccg
     4381 aaacgggcgc ggtcgacctg atcggctacc tgtcgcagtc gagggtcctc cagggggggc
     4441 cggactactt cggtcatgag gccacacttt ccaatacgtc gtccgagatg tcgaagttcg
     4501 catagacgtc ctgcacgtcg tcgagttctt cgagggagtc gatcaaggtc agtaccttct
     4561 tcgcgtcgga tacctccaga gggacggtcg tcttcggtac ctgagtcaac tcgtacgact
     4621 cgatcgagac accggctccc tccagtgcat cgcgaacggg cttgagcgca tccgtcgggg
     4681 tgatcacttc gatcgaatcc tccgatccgc gcacgtcctc ggcccccgca tccgccgcga
     4741 tcatgaaaac ctcgtcctcg tccacgccgt ccttcagcac gatcacgaca cccttcggct
     4801 cgaacatcca cgacactgcg ccgggctcgg ccatcttgcc ggcgtgtttc gtgaacaagc
     4861 ttctaacgtc ttgggcggtt cggttgcgat ttccggtgag acaactgacg aggacggcca
     4921 ctccgccggc tccgtaaccc tcgtaagaga tctcctcgta tgtctcaccc tcgagctcgc
     4981 cggtccctcg tttgatggcg cgctcgatgt tgtcgttggt gacgctcgac cctttggcct
     5041 tctcgaccgc cgaagcgagg gtcgggttcg cgtcgaggct cccgccgccc tcgcgagccg
     5101 ccacttcgac ggctcttaga agcttggccc acagcttcga gcgggccgcg tcggccgcac
     5161 ctttcttgcg cttgatcgtc gaccacttgc tgtgaccgga catccgtcac cccaccatgc
     5221 ttaggaacat ctcgtgaacc ctgtaatcgt ccgtcatctc ggggtggaac gttgacgcca
     5281 gaagattccc cctgcggacg aggaccggcc tgccctccag ctcggccaga acctcgacgt
     5341 cgtcgcccac tcggcggatc accggagccc ggatgaacac cgcccggaag ggctcggcca
     5401 cgccccggac cacgagatcg gtctcgaagg agtcgatctg ccgtccatag gcgttgcgct
     5461 cgatcgccat gtccagcagt ccgaaacgat gaggagcatc ctgcgcccca tctatctctg
     5521 tcgccatcag gatcgcgccg gcgcacgtgc cgtacagcgg catgccggcc ttcgctcgat
     5581 cggcgatcgg ttgcagcaac ccgaaccggt cgagcaactt gccgatcgtc gtggactctc
     5641 ccccgggaac gatcaacgcg tcgacgctct caagctgagc ttcgtgcttg acctccagcg
     5701 gggtaccgcc cgccgcctcg acggcgcgca cgtgttcccg cacgtcaccc tggagtgcga
     5761 gaaccccgac cttcaaaagg tctaccagcc ccggcgcgcc agccgttgct cgtcggagat
     5821 gtcgtccagg ttgatcccga ccatcgcttc accgagcccg cgcgagacct tggccacgat
     5881 ctcgggttct ttgaagtagg tcgtcgcttc aacgatcgcc cgagctcgtg tcgccggatc
     5941 gccggatttg aagatgccgg agcccacgaa gacaccgtcg gcaccgaggt gcatcatcag
     6001 agcggcgtcg gcaggagtcg cgatcccacc ggcggtgaac agaactactg ggaacgtccc
     6061 cgtctcggag atctctcgaa cgagatcgaa gggcgcgcgc agctccttgg cctccgccat
     6121 cagctcttcg cccccgaggg tcgcgagttt cctgataccg gatgtgatcg agcgcatgtg
     6181 acgaacggcc tcgacgacgt tgccggtccc ggcctcacct ttcgagcgga tcatcgcggc
     6241 cccctcaccc aacctgcgca atgcctcgcc cagattcgtc gcgccgcaca cgaacgggac
     6301 gtcgaagtcc cacttgttca cgtgattgct ctcgtcggcc ggcgtaagca cctcggactc
     6361 gtcgatgtag tcgaccccga gggattgcaa cacctgagct tcggtgaaat gaccgatccg
     6421 gcacttggcc ataacgggga tcgagaccgt gtccatgatc tcggagatct tctcggggtc
     6481 ggccatgcgg acgactccgc cttcggcccg gatgtcggcg gggacgcgct cgagtgccat
     6541 aaccgcgacg gctcccgcct cttctgcgat cttcgcttgc tcggcggtcg tgacgtccat
     6601 gatcacgccg cccttcagca tctcggctag gccgcgcttg acgcgtgccc caccggtgtc
     6661 acgggagatg tcggccatgt cgaaactcca ggctcgcggt ccgatccgag agtcgatcct
     6721 accgccgcgg ttccctactt aaaggagaag gagcccttca cttgcccctt gtgagaggga
     6781 acaagctcga tccagacggg gccgggcgcg aacacgaact cgtcaccgtt gcgcttctcg
     6841 aggatgggga agcggccttc gtcctcgatc tcccacgtgc ccttcatcac tttcccgtcc
     6901 cggaacagaa gcgcgcggcc cttgcccacc aactggatgt cgggagaagg gttgcccgca
     6961 ctgtcgacga tcttgggcga gttattgacc atgacttcct gaaccagcac gttggggacc
     7021 gctatctgcc cgttcgtctc gctcatgaac ggcgcaccgg cctcgtagcg gtgccatgcc
     7081 cctttcttcc agcgatactc gatctcattc gaggccgtga agtggatcgc gacgtgacgc
     7141 gccttcttca agccatccgg aaggtcaccg aactcgaaca gatccttctt cggaccgtcg
     7201 gctttcttag ccggaggaag cttgcggatc ttcttggtgt cgccgaacag gttgtgtatc
     7261 tcgagaacgc cgggcggcac gcggtagaga gcaccgacac cgtcgttctc ttgcagcgcg
     7321 atcaggttct gcctgacgag ctcgcgctcg acgatcgaat tcccacccga gaacgccagg
     7381 atgcgcgtgt agggctttgc gatcttcggg tcgtcgaagc gcgcgctgcg caccggacca
     7441 accttcttcg agttcttaca gtgatagatc gccatgaatc gggtgatgcc accctcgacg
     7501 atctcctcgt agacaacgtc ggcatcttcg agcccgctct ggggccgggc ggccgacgaa
     7561 ttctcgacct tcaccgccac ggccggcttc cggatatcga cccccgacgg gatgtcctcc
     7621 ccggtcaatg gacagaccgg atcgggtgtg ggcgtgggag tgggcgtgga tgcgaccgtc
     7681 ggctcctcct cactgcaagc tgcagagaag accagaccga acaacatagc cacgacgagc
     7741 gatctcttga tgcgcactat tacctacttc tttttgaagt cgaacgagcc tttgacctcg
     7801 cccttcttgc tgggcaacag ctcgatccat atcgaaccgg gcgcgaacac gaattcgtcg
     7861 ccttctttgg tttcgaaggt cacggcatcg ccgacgtcct cgcgcgacca tttgcctttg
     7921 atgacctgcc catcgcggaa caggaacgct cgccccgagc cggtctcgtt tgcgatctca
     7981 agcgatggat tccccgctac gtccgtgatc gtattcgaga agattacctc gtgctcctcg
     8041 ataagaacgt tgttgacgat gatctggtca cccgactcgg ccatgaacgg ctcgtcggcc
     8101 tcgaatctca gccaaccgtc gtccgaccag tcgtaacgga tcgtgttgac cggattgaaa
     8161 ttgatctcga tctgcttggc cggcttggtc ttgccttcga tgtccccgaa ggtgaacgtg
     8221 tcgccggggg gaggatcgtc gaactccttg gtcccgagct tgcgaaggat cttcgtgtcc
     8281 gcgtacaggg tgtgctccat gctgatcccc gagcgctcga tgcgctgcaa gccctccccc
     8341 gcctcggttt cgtccagacg gacgacctcg gcctcctcga gcgcccgcag taccggagcg
     8401 ttctggcccg agtaggccag gatccgtgtg atcgcgccca tgatcgcggg atccacgaca
     8461 cgggcgctac gtacgggtcc ggctttcgtc gaatcggtgc agtggtacac cgccatgaac
     8521 cgggtcacac caccctcgac cagctcctcg tacacgagct cggcgtcctc gagacccgac
     8581 agcgggtaag cgatcgccgc gttctcgatc ttcaccgcga ctgcgggccg gtccaacgca
     8641 gcttccttcg ccggttccga cccacttagt ggacaggtcg cgggcgcggt tatctctttg
     8701 atgatcggta tgtcgctcgg tctgttgccg agcaagaaga acgccccggc cgacagcacg
     8761 acgatcccgc cgactgcgcc gagcgccact ttcacgcgtt tatccatcgc gacgcggttc
     8821 ctccttagtc aactcttggt ctgagtgttt gcgttcaccg ctcggtttgc cgctcgggga
     8881 gaacgcgaaa acgcgcctct cggcgccccc tcaagttaac acctggagac cgggaaattg
     8941 tggtttttag cccgctttag agatctccca gcgcgtctat ccggctcacg actgggtagt
     9001 gcgaggggct gtgagcgacg aaccacaagc acgcggtcgg gccctgtact gggccggcgt
     9061 gctgtatcgc ccgcgccagt ccgggtggat agcgcgtgat cgccgccgtt tgaaggtcgt
     9121 cttcggaacc gaccttcggc gcgaagcggc tggccgaccg gcccaatcga gccgcgagtt
     9181 ccgagaagaa gagatggccc gtatggatac gcacgaggca gtgcgcggca acggcttcct
     9241 gttcggtgcg gctgtaggtg tcgaggagcg aacgcgtcac gccgatcacg ggtgggtgtc
     9301 ctcgcagcac gatggcattc ggcttgtcgc ccgtgacgag gaatatctca ggtcgaggtg
     9361 attcgagtcc ctcggccaag ccttcgacca ggttgcggaa gcgagcgccc gattcgtcca
     9421 gagggacggc gtcgaatctc ttgaggatct gcttacggag ccttccgacg tacacaccca
     9481 tcgccatgct gacgatcgtc agggtcagag cgaacccgac ccatccccca ccgaaaggga
     9541 aagacagggc ggcagatacg agcactaacg gaccggccag taccacgaag agccagatcc
     9601 tcttgccgag cggataggtg ggagggtctc caccccaatc gggcaggtcg cgtgccgcgg
     9661 tcgccatcag taacccgggc ccgacttcag caaggtcgct gcttcgctgt gat
//
LOCUS       DATMED010000289         6080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1381872, whole genome shotgun sequence.
ACCESSION   DATMED010000289 DATMED010000000
VERSION     DATMED010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381872"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VNP73_07380"
     CDS             complement(<1..516)
                     /locus_tag="VNP73_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65778.1"
                     /translation="MLEHLSVLVVGGDASVRIALNDALGEGGSAMFLAGDVGPAELLA
                     SSAYADLIVAVISDHPDPPALLGACRDLGLQRRTLAIGDALDRSAAQEAVALGVAGYI
                     TAGSDASHLVKAISHVVDNGVILDPQAADAYSGQISLDFGSGLKLSAARAFASALELK
                     DTYTGGHAERVT"
     gene            complement(517..972)
                     /locus_tag="VNP73_07385"
     CDS             complement(517..972)
                     /locus_tag="VNP73_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL65779.1"
                     /translation="MSDSLRVLVVDDEPDVRTVVGMNLDLAGMEHGEAADGGEAIDML
                     NSGDWDACILDLGLPDVDGLSVLQDLKESGRINDISVVVLSARGSPDAAVQAMQLGAH
                     AHLTKPFSPASVAQIVQELTQLTPDDRASRRSEMIDRAVELERLGMRTI"
     gene            complement(969..2168)
                     /locus_tag="VNP73_07390"
     CDS             complement(969..2168)
                     /locus_tag="VNP73_07390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWL65780.1"
                     /translation="MTKRPPPAAKGSSPTNNGKPVVLVVDDDPDLRTVAEMQLSLRGF
                     HVIQAESGEQALELARTKSPDVILLDMMMPGMSGADVLRALHADPDLEDIPVIFLSAV
                     SGLEDRVQGLEGGAVDWITKPHDPRELIARVGAAARTKARQEEMKLQVGNDPITRLPM
                     RKAFEESLLLELNRATRNRNPFCIFLVDIDHLSSVNDRLGELAGDQLLREVASVLRST
                     LRNSDEVYRYGDDEFAVLLPDVEVGAAFQAAERCRVEIETITSDGVPTAASFGVAQSS
                     GGRSAEDILSRAEIALYRAKESGGGCIWRADDPRRHGLNPVALSEELTEREWDVLVHL
                     SHRRTEHEIARRLGISRGTVRSHKARIRRKLHVSPDIRLSDFVRSNLKDLIDRLPGAI
                     DKEGVQS"
     gene            2273..2722
                     /locus_tag="VNP73_07395"
     CDS             2273..2722
                     /locus_tag="VNP73_07395"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HWL65781.1"
                     /translation="MEEPLPGGEEPLDDDEREALRQDLQDVRVLKGLLGPRGIKGVVF
                     YCPDCDEDHYLAWDLLAGNLKELLDAGESPVHEPAYEPDPDDYVSWDFARGFLDGYES
                     SGEEWDDTVNKLVLELRDRGWGPSEVKGLLAALELTASMQGPSESGD"
     gene            2715..3476
                     /locus_tag="VNP73_07400"
     CDS             2715..3476
                     /locus_tag="VNP73_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWL65782.1"
                     /translation="MTRTIAFANQKGGVAKTTTTLSIAVALAELGKRVLAVDLDPQGA
                     LTYSMGVDPDTLDETINDVLIRRLPVEKVVITREIDCVPANIDLAGAEAVLLAKTGRE
                     YALQRALREIEGAYDYVLIDCPPSLGILTINGLTAAQYVVIPLQCEALSHRGVGQLIE
                     TMGDIRHFTNPDLRVVGLIPTLFDGRSRHTQEVLADIGSRYEVEVLEPPVRKSIKFAE
                     ASQAGRSILSYAPTHPGAIAYRELAGRIDRAIDEV"
     gene            3799..4290
                     /locus_tag="VNP73_07405"
     CDS             3799..4290
                     /locus_tag="VNP73_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65783.1"
                     /translation="MARKSAKELKKARKQIGKAADEVRKVGGKALKTAQKTAKEVAKT
                     RDAKTAKKQVKVAQKAVASAADAGLTLAKTALKSGQKSAKKLAGSRDVKKAKKQVRSA
                     ASQGKKAAKATSKTARKSGTKAVKQARSTSATAAKNAQKRTAAARKTAQKRASQAKKR
                     ATS"
     gene            4307..5122
                     /locus_tag="VNP73_07410"
     CDS             4307..5122
                     /locus_tag="VNP73_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HWL65784.1"
                     /translation="MNPDPPSGPQGSGPTEVPPELRPRGLSGSWRGQLWAPPDEPFKA
                     LTVAGPLKVLLAIGVLLDVLIVAVWYLRYSFAQRAVSGGSFTDAEVTTSDMMLGLTGI
                     GQLLVFTTTGILFIVWVRRAYRNLHPLGVQKLRFKAGWAVGGWFVPFLNAVRPKEIVN
                     DIWRSTDPDAPREQEGPGLGRPVEQIVNWWWASYLVSQAAYQFIFRMPIPETAEQLVN
                     EAQVGMVSDALGAVAGVTAFLAVGAITKRHDERAAALGVTGGPPALPVQESVR"
     gene            5173..>6080
                     /locus_tag="VNP73_07415"
     CDS             5173..>6080
                     /locus_tag="VNP73_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWL65785.1"
                     /translation="MAGSGGTTHPSRAQWLRRLARRRRDTVGFVLSGGGPLAALQVGA
                     LRALYAREIRPDLVVGTSAGALNAAWTAWDPTPSGITHLEWTWRNMKDGDLFPGGRFK
                     ASWARMLVKGNRVFENSGLKRQIETTLGWDSRFEDAQIPLAVTTTDLESGEERVFSSG
                     PLALPLLASAAMPGIFPPVEIDGRMYIDGGVADNVPIAPAVAMGAKTLYVMDSTSHSH
                     QRRPLARPIDYLLHAFSLARGQRLSLDLAHYRERVKIVMLPAPKLEIFVPFASLEHTA
                     HLINLSYEHTLSFLEGGIPRETGITS"
BASE COUNT         1131 a         1909 c         1937 g         1103 t
ORIGIN      
        1 cgtcacgcgt tcggcatgac caccggtgta ggtgtctttc agctcgaggg cggacgcgaa
       61 cgcgcgcgcc gcgcttagtt tgagtcccga gccgaagtcc agactgatct gaccggagta
      121 ggcatcggct gcctgaggat ccaggatcac accgttgtcc accacgtggc tgatcgcttt
      181 cacaaggtgt gatgcatccg aaccggcggt gatgtaaccg gcgaccccga gtgctactgc
      241 ctcttgcgcg gccgaccgat ccagggcgtc cccgatcgcg agggtgcggc gctgcaatcc
      301 gagatcccgg caggcaccga gcagtgccgg aggatcgggg tggtctgaga tcacggcgac
      361 tatgaggtct gcgtaggccg acgacgccag cagctccgcc ggacccacgt cgccggcgag
      421 gaacatcgcg gagccgccct caccgagcgc gtcgttgagg gcgatacgaa ccgacgcatc
      481 gcctccgacg acgaggacgc tcaagtgttc gagcatctag atagtcctca tccccaagcg
      541 ctcgagctcg accgctcgat cgatcatctc ggaacgtcgt gatgctctgt cgtcgggagt
      601 gagttgagtg agctcctgca ctatctgcgc caccgaagcc ggggagaacg gcttcgttag
      661 gtgggcatgg gcgccgagct gcatcgcctg cacggccgca tcggggctcc ctctcgccga
      721 gaggacgacg acgctgatgt cgttgatgcg accgctctct ttcagatctt gcaggacgct
      781 gagcccgtcg acatccggca gtccaagatc caagatgcac gcgtcccagt ctccagagtt
      841 gagcatgtcg atggcttcgc ccccatctgc tgcttctccg tgttccatcc ctgcgaggtc
      901 gagattcatc ccgaccacgg ttcggacgtc gggctcgtcg tccacgacca agactcgcaa
      961 tgagtcgctc aactctgcac cccctcctta tcgattgccc ccggaagccg gtcgatcaga
     1021 tctttcaagt tgctgcgcac gaagtctgaa agacggatgt cgggagacac gtgcaatttc
     1081 cgacggatac gggctttgtg actcctgacc gttccgcggc tgatgcccag cctgcgcgcg
     1141 atctcgtgtt ctgtgcggcg atgcgaaaga tgaaccagga cgtcccactc gcgttcggtc
     1201 agttcttcgg agagtgcgac cgggttcagc ccgtgacgtc gtgggtcgtc ggcccgccag
     1261 atacagccgc ctccggattc cttagcgcga tagagggcga tctccgcgcg cgacaggatg
     1321 tcttcggcac tgcggccccc cgacgactgc gccaccccga acgatgccgc ggtgggaacg
     1381 ccgtcggacg tgatggtttc gatctccacg cgacagcgct cggccgcctg gaaagcggcg
     1441 ccgacctcga catcggggag aaggacggcg aattcgtcat ccccataccg atagacctcg
     1501 tcggagttgc gcagtgtcga acgcagcacc gacgcgacct cgcgtaacag ttggtctccg
     1561 gcaagctctc cgagccggtc gttgactgag ctcaagtggt cgatgtcgac caggaagatg
     1621 cagaatggat tgcggttccg agtcgctcgg ttcagctcga gaagcagact ctcttcgaac
     1681 gccttgcgca tcggtagccg agttatggga tcgttgccca cctgcaactt catctcttcc
     1741 tggcgggctt ttgtccttgc cgcggccccc actcgcgcga tgagctcgcg cgggtcgtga
     1801 ggtttcgtga tccagtcaac ggcgccacct tcgagcccct ggacgcgatc ctcgagtccg
     1861 gagaccgccg agaggaagat cacagggatg tcttccaggt cggggtccgc atgcagcgcc
     1921 cgcagaacgt ccgcgccgga catccccggc atcatcatgt cgagaaggat cacgtcgggt
     1981 gacttggtcc gagcaagctc cagcgcctgc tcaccactct ccgcttggat cacatggaat
     2041 ccccgcaggg acaactgcat ctcggcaacg gttctgagat ccgggtcgtc atccaccacc
     2101 agcacgactg gtttgccgtt gttggtggga gatgaccctt tggctgctgg cggcggccgt
     2161 ttggtcatga tgtcagcgtg acagcgagcc gagccgtgtg gaatcaacac atcggactaa
     2221 ttcgtatgac ccgtcagaac tagtcccccg aagcgggact tacgatgagg gggtggaaga
     2281 gcccctgccc ggcggcgaag agccgctcga tgacgatgaa cgcgaggccc tgcgccagga
     2341 tctccaggac gttcgtgtcc tgaaaggtct actgggcccg cgggggatca agggcgtcgt
     2401 cttctactgc cccgactgcg acgaggacca ctatctggcg tgggatcttc ttgccggtaa
     2461 cctgaaggag ctgctggacg cgggcgagtc tccggtgcac gagcctgctt acgagccgga
     2521 tcccgacgac tatgtgtcgt gggacttcgc tcgcgggttc ctcgacggct acgagtcgtc
     2581 gggcgaagaa tgggacgaca ccgtgaacaa actcgtattg gagctacgtg atcgcggatg
     2641 gggaccctcc gaagtgaagg gactgctggc cgccctcgag ctcaccgcct cgatgcaagg
     2701 accgagcgaa tccggtgact agaactatcg cgttcgcgaa tcagaaaggt ggtgtcgcca
     2761 agacgacgac caccttgtcc atcgcggttg cgctggccga gctgggcaag cgcgtgcttg
     2821 ccgtcgatct tgacccgcaa ggcgccctca cgtattcgat gggtgtcgat ccggacactc
     2881 tcgacgagac gatcaacgac gttctcatcc ggcggcttcc ggtggagaag gttgtgatca
     2941 cccgggagat cgactgcgtc ccggccaata tcgatctcgc gggggccgaa gcggtacttc
     3001 tcgcaaagac cggacgcgag tacgcgttgc agcgggcgct gcgcgagatc gagggtgcct
     3061 acgactacgt tctgatcgat tgcccaccct cgctggggat ccttacgatc aacgggctca
     3121 cggccgctca gtacgtggtg atcccgttgc agtgcgaagc gttgtctcac agaggtgtcg
     3181 gtcagctgat cgagacgatg ggggacatcc ggcatttcac caatccggat ctgagggtcg
     3241 tcggattgat ccccacactg ttcgacgggc gttcgcgaca cacgcaggag gtcctcgccg
     3301 acatcgggtc ccgctacgag gtcgaggtct tggaaccacc ggtccgcaag tccatcaagt
     3361 tcgcggaagc atcacaggca gggcgctcca tcctctcgta cgctccgact cacccaggtg
     3421 ccatcgcgta tcgcgagctg gccggcagga tcgaccgcgc gatcgacgag gtataggcat
     3481 ggtcggccgg gacgaggcgc ccactaagca cgccctttac agcaagcccg agcgacgggc
     3541 actcacctgc acggtcgaat gctcggcgtg ccgggaggag accagggtct cctaccttga
     3601 gctcgcggcg ctcctgttcc cggttcactt ccatgtccct ctggtgcgct accaccactc
     3661 ttggttgcga tgccccgcct gcggccgccg tacttgggtg cgcgtgcatc tggaccgcta
     3721 gaaaactggc ggggcctgaa tagcttggcc tcccgcgggt aacctcgcca catacattgg
     3781 gatcgaggga gggccaccat ggcacgcaag tccgccaaag agctgaagaa ggcgcgcaag
     3841 cagatcggaa aggctgccga cgaagtgcgc aaggtcgggg gcaaggcact caagaccgcg
     3901 cagaagacgg ctaaagaagt tgctaagacc cgcgatgcga agaccgccaa gaaacaggtg
     3961 aaggtcgctc agaaggcggt tgcatctgca gcagacgcag gcctgactct tgcgaagacc
     4021 gcgttgaaga gcggacagaa gagcgccaag aagctggccg gctcgcgcga cgtcaagaag
     4081 gctaagaagc aggttaggag cgcggcttcc caaggaaaga aagcggccaa agcgacgtcc
     4141 aagacggccc gcaagtccgg caccaaggcc gttaagcagg cgcgttcgac cagtgcgacg
     4201 gccgctaaga acgctcagaa gcgaaccgct gctgcgcgca agacggcgca gaagcgcgcg
     4261 tcgcaagcga agaagcgcgc gacgagctga cctcgtctaa ccgagcatga accccgatcc
     4321 gccgtccggc ccccaggggt ccggacctac ggaggtacct ccggaactca ggccgcgcgg
     4381 gctctcgggg tcgtggcgcg gtcagctatg ggccccgccc gacgagccct tcaaggccct
     4441 cactgttgcc ggtccgctca aggttcttct cgccatcggt gtcctgctcg acgtcctgat
     4501 cgttgcggtg tggtatctcc gctactcatt tgctcagcgt gcggtttccg gagggagctt
     4561 caccgacgcg gaagtgacga ccagcgacat gatgttgggg ctgacgggga tcggacaact
     4621 actcgtcttc acgaccacgg ggatcctgtt catcgtgtgg gtccgtcgcg cctatcgcaa
     4681 ccttcacccc ctcggggtcc agaagcttag gttcaaggca gggtgggccg ttggaggctg
     4741 gttcgttccc ttcctgaacg ccgtgcgacc caaggagatc gtcaacgaca tctggcgttc
     4801 gaccgatccg gacgctccgc gagagcagga gggaccgggg ttgggacgcc cggtggaaca
     4861 gatcgtcaac tggtggtggg cctcatacct cgtctcgcag gccgcgtatc aattcatctt
     4921 tcggatgccg atcccggaaa cggcggagca gctcgtgaac gaagctcagg tcggcatggt
     4981 ctcggatgcc ctcggcgcgg tagccggtgt gacggctttc ctcgccgtcg gtgcgatcac
     5041 caagaggcac gacgaacgtg ccgcagctct tggcgtgacc ggagggcccc ccgccctgcc
     5101 ggtccaagaa tccgttcgct aattgctcgc atattgctca cataagggcg cgcgatagcc
     5161 gattattgac ttatggcagg aagtggaggg acgacgcatc cgtcgcgcgc gcagtggctc
     5221 cgcaggttgg ctcggcgtcg ccgcgacacc gtgggcttcg ttctgtcggg gggcgggccg
     5281 ttagcggctc ttcaggtggg cgctctacga gccctgtatg cacgagagat aaggccggac
     5341 ctcgtcgtcg ggacctcggc cggcgctctg aacgcggcct ggaccgcatg ggaccccacc
     5401 ccaagtggga tcacgcatct cgaatggacg tggagaaaca tgaaggacgg cgacctgttc
     5461 ccggggggcc gcttcaaggc gtcgtgggcc cggatgctcg tgaagggcaa ccgcgtcttc
     5521 gagaactcgg gtctcaagag acagatcgag acgacccttg gctgggattc tcgattcgaa
     5581 gatgctcaga tcccactcgc tgtgaccaca accgacctcg agagtggcga ggagagggtg
     5641 ttcagctccg gccccctcgc gctaccgctc cttgcgtcgg ctgcgatgcc gggcatcttt
     5701 ccgccggtcg agatcgatgg ccggatgtac atcgacggag gagtggcgga caacgtgccg
     5761 atcgcaccgg ccgtggctat gggagccaag accctctacg tcatggactc gacatcgcac
     5821 agtcaccagc gccggccgct ggcccggccc atcgactatc tgttgcatgc gttcagcctg
     5881 gctcgcggtc agcgcttgag tctcgacctg gctcactacc gcgaacgggt gaagatcgtc
     5941 atgctgccgg caccgaagct cgagatcttc gtcccattcg cgtcactcga gcacaccgct
     6001 cacctgatca acctgtccta cgagcacacg ctcagtttcc tcgagggtgg gatcccccga
     6061 gagaccggca ttacgtctga
//
LOCUS       DATMED010000290         4379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4071365, whole genome shotgun sequence.
ACCESSION   DATMED010000290 DATMED010000000
VERSION     DATMED010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4071365"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..687)
                     /locus_tag="VNP73_07420"
     CDS             complement(55..687)
                     /locus_tag="VNP73_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HWL65786.1"
                     /translation="MTTLWLVRHAVTSHTGKKLSGWLEGIDLSEEGHQQAEAAAEVLA
                     KIPLEAVYASPLERTVQTARAIARTHRLSVRVARDLGEVQYGKWTNRSMKVLARTKLW
                     TKVQQWPSGARFPDGESLREVQARAIVAVEQLVEEHPRQHICCVSHGDVIRLLMAHYL
                     GVHMDLFQRIAIAPGAISSVAIGERGPIVLAMNVVPSMFVRSPAEVEEEE"
     gene            complement(694..2628)
                     /locus_tag="VNP73_07425"
     CDS             complement(694..2628)
                     /locus_tag="VNP73_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL65787.1"
                     /translation="MPEFVWEPTSDYVENANVTRLMRKHGIDSFDELVKKSQDDIEWY
                     WPAAIEDVGIDFFEPYQQLLDTSEGIQFPRWFVGGKVNLTYNCVDRWAESRQGNPAIV
                     WEGEDGATRTVTYSMLSVAVSKVANGLRSLGVGEGDRVGVYMPMIPDAVIAMYAIAKI
                     GAIYMPIFSGFGPPAVATRLNDAQAKVLFTADGFWRRGTKVQMKEAADEAVAESPSIE
                     KVIVSRRFADVEPTMGPKDVYWSEIFRDQPGELDAPQLDPETPFMIAYTSGTTGKPKG
                     SVHVHGGFLVKIAQEVCYQLDVKLDDLLYWVTDMGWIMGPLEMVGGHCNGAAVFMYEG
                     APNHPKPDRLWEMIERHRINILGISPTLVRALMPAGEEHVTSHDLSSLRILASTGEPW
                     NPEPYSWFSDVVGGGRLPIMNLSGGTEIGACFLGQCPVIPTKNCSLGTPSLGMAMDVF
                     DPDGKPVRGEVGELVCTKPWPAMTRGFWGSKQRYLDTYWSRWDGIWVHGDWASVDDDG
                     FWFLHGRSDDTLNIAGKRIGPAEFESAAVEHPSVVECAAVGIPHEVKGETVWCFCILK
                     PGVTPTDELAAEIKRTISGDLGKAFTPEQIRFVEELPKTRSAKILRRAIRAKVLGEDP
                     GDLSSLENPSALEGIEKSLA"
     gene            complement(2697..3908)
                     /locus_tag="VNP73_07430"
     CDS             complement(2697..3908)
                     /locus_tag="VNP73_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65788.1"
                     /translation="MKVGKFAALVSASLVAAMLIPSTTASANHNANLDIQAGEYLFET
                     KVASGESMRFFPADLTVHKGDTLTFRGDFHTALFLPTSATDADAWIAENATAPDDPYS
                     FIKGNPDNQTYPLKLWGDNPFTPAPCGSEDAPCAYGGDEVIDSGLLFNYYDFSGGPDV
                     PPVNNGFTVTVDANPGDVFWVICGVHPKMRMRVVVVGPNEDSTTQESIDTYTTTTVNE
                     DRRAATELHESMSSSHPSQMVDGKKVWTAFAGYDAEDFSLFGMYPKKINVKRGQSVEW
                     RFASLNFEVHTATLANKKARRLGHRGQPILCDPNGDGESGGETMPPPEAFPPQCSEGA
                     AEQVLAAQMLSVVGDGTFKNVKDLENSGVRGAMTPTRDPYVLKIGARSGSTYTYLCAI
                     HPFMIGKIRVK"
     gene            complement(4002..>4379)
                     /locus_tag="VNP73_07435"
     CDS             complement(4002..>4379)
                     /locus_tag="VNP73_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HWL65789.1"
                     /translation="VLAEHSFEVEPGTRLIGVAGTVTQLAALKLGLDAYEPELTHHSV
                     LTHSDVRKLARRLEILPFEQRKQVKGLVPGRVDVIVAGAEILLCVMDAAGATDLLVSE
                     RDILDGLVLGLLDSSEPDISESL"
BASE COUNT          807 a         1389 c         1310 g          873 t
ORIGIN      
        1 cgggtgtgcc ggtgtaatcg gccaggaaca cctccggttg gatctcgaga gggatcattc
       61 ctcttcctcc acctccgccg gcgatctcac gaacatcgac ggtacgacgt tcatcgccag
      121 cacgatcggc cccctctcac cgatcgcaac cgaggagatt gcccccggcg cgatcgctat
      181 ccgctgaaag aggtccatgt gcaccccaag gtagtgcgcc atgaggagcc ggatgacgtc
      241 gccatgcgag acacaacaga tgtgctgccg gggatgttcc tcgaccagct gctccaccgc
      301 gacgatcgcc cgagcttgca cctctctcag tgactcgccg tcggggaagc gcgcgcccga
      361 aggccattgc tgcaccttcg tccacagctt cgtccgggcc agaaccttca tcgaacgatt
      421 cgtccacttc ccgtactgaa cctctccgag atcgcgggcc acccgcacgc tgagccggtg
      481 ggtacgcgcg atcgctcgtg cagtctgaac ggttcgttcg agtgggctcg cgtacaccgc
      541 ttcgagcggg atcttggcga ggacctctgc agccgcctcg gcctgctggt gaccttcctc
      601 ggagagatct atcccttcca accatccgga cagcttcttg ccggtgtggg aggtgactgc
      661 gtggcgaacg agccacaggg tcgtcatctc ggctcaggcg agggacttct cgatgccctc
      721 gagcgcactg ggattctcga gggacgagag atctccggga tcctcgccga gcaccttcgc
      781 gcggatcgct cggcgaagga tctttgcaga gcgtgtcttg ggtagctctt ctacgaaacg
      841 gatctgctcg ggagtgaatg ccttgcccaa atcacccgag attgttcgtt tgatctcggc
      901 cgcaagttcg tcggtcggtg tgacgccggg cttgaggatg cagaagcacc acacggtctc
      961 cccttttact tcgtgaggga tgccaaccgc agcacattcc acgacgctgg ggtgctccac
     1021 tgcagccgat tcgaactcgg ccgggccgat gcgtttcccg gcgatgttca gcgtgtcgtc
     1081 gctgcggccg tgcaggaacc agaacccgtc gtcatcgacc gaagcccagt ccccgtgaac
     1141 ccagatcccg tcccagcgcg accagtaggt atccagataa cgctgtttgc ttccccagaa
     1201 tccccgtgtc atagcgggcc acggcttcgt gcacacgagc tcgcccacct cgccccgcac
     1261 gggcttcccg tcgggatcga atacgtccat ggccatgccg agcgacggag ttccgagcga
     1321 acagttcttc gtcgggatca ccggacactg ccctaggaag caggcgccga tctcggtgcc
     1381 gcccgacagg ttcatgatcg ggagacgccc gcctccgacg acgtcggaga accacgagta
     1441 cggctcgggg ttccacggct cgccggtcga ggcgaggatg cgaaggctcg atagatcgtg
     1501 gctcgtgacg tgctcttcgc ccgccggcat cagtgctcgt acgagcgtcg gcgagatgcc
     1561 gaggatgttg atccggtgcc gctcgatcat ctcccacagc cgatcgggct tcggatggtt
     1621 gggcgctccc tcgtacatga agaccgcggc accgttgcaa tggcccccga ccatctcgag
     1681 cggacccatg atccagccca tgtcggtgac ccaatagaga agatcgtcga gtttcacgtc
     1741 gagctggtag cagacctcct gtgcgatctt gaccaggaat ccaccgtgaa cgtgcacgct
     1801 tcctttcggc ttacccgtcg tgcccgaggt gtaagcgatc atgaaaggtg tctcggggtc
     1861 gagttgagga gcatcgagtt cgcccggttg atcgcggaag atctcgctcc aatagacgtc
     1921 tttgggcccc atggtcggtt cgacgtcggc gaaccgacgg ctgacgatca ccttctcgat
     1981 gctcggagac tcggcaaccg cttcgtctgc agcttccttc atctgcactt tcgtgccgcg
     2041 ccgccagaac ccgtccgcgg taaacagaac cttcgcttgc gcgtcgttga gccgcgtcgc
     2101 cacggcgggg gggccgaaac cggagaagat cggcatgtag atcgccccga tctttgcgat
     2161 cgcatacatc gcgatgacgg cgtcgggaat catcggcatg tagacaccga ccctgtcgcc
     2221 ctcgccgacg ccgagactcc gaaggccgtt cgccaccttg ctcaccgcga ccgagagcat
     2281 cgaatacgtc acggtgcggg tcgcgccgtc ttcgccctcc cacacgatgg cggggttgcc
     2341 ttgcctcgac tcggcccagc gatcgacgca gttgtaggtc agattgacct tgccgcctac
     2401 gaaccagcgc gggaactgga tgccttcgga cgtgtccagc agctgttggt agggctcgaa
     2461 gaagtcgatc ccgacgtcct cgatcgcggc gggccagtac cactcgatgt cgtcctgtga
     2521 ctttttgacc aactcatcga aggagtcgat cccgtgcttg cgcatgaggc gcgtcacatt
     2581 ggcgttctcg acgtagtccg aggttggttc ccaaacgaat tccggcacga gcgggaggct
     2641 accgcagaca caaaaagaga gaggcggccc gggggccgcc tctctcagtg acactgctac
     2701 ttgacgcgga tcttcccaat catgaagggg tggatcgcgc agagatacgt gtatgtcgaa
     2761 cccgagcgcg caccgatctt gaggacgtac gggtccctcg tcggtgtcat tgcgcctcgg
     2821 actccggagt tctccaggtc cttgacgttc ttaaaggtgc cgtcacccac taccgacagc
     2881 atctgcgccg ccaaaacctg ctctgcagca ccctcggagc actgtggcgg gaaagcctcg
     2941 ggcggaggca tcgtttcacc gcccgattca ccgtcgccgt tcgggtcgca gaggatcggt
     3001 tgccctctgt gtccgaggcg ccgggccttc ttgttagcca aggtcgccgt gtgcacctcg
     3061 aagttaaggg acgcgaagcg ccactcgaca ctttggcctc gcttcacgtt gatcttcttg
     3121 gggtacatcc cgaatagcga gaagtcttca gcgtcgtaac ccgcaaaagc ggtccagacc
     3181 ttctttccgt caaccatctg actcgggtga gaggaagaca tgctctcgtg cagttccgtc
     3241 gctgcccgac gatcctcgtt caccgtagtg gtggtgtacg tgtcgatcga ctcctgggta
     3301 gtggaatcct cgtttggacc cacgaccact acgcgcatgc gcatcttcgg gtggacgccg
     3361 cagatgaccc agaagacgtc tcccgggttt gcatcgaccg taacggtgaa gccgttattg
     3421 accggtggaa cgtccggacc tccggaaaag tcgtagtagt tgaagagcaa gcctgagtcg
     3481 atgacttcgt cgccgccgta agcacagggc gcgtcctctg aaccacatgg agccggagtg
     3541 aacgggttgt caccccacag cttcaaggga tacgtctggt tgtcggggtt tcccttgatg
     3601 aacgaatacg ggtcgtcggg tgccgtcgca ttctcggcga tccacgcgtc ggcatcggtc
     3661 gcgctcgttg gaaggaacaa cgcggtgtgg aagtcccctc tgaacgtcag ggtgtcgccc
     3721 ttgtgaaccg tcaggtccgc cgggaagaac ctcatcgact cgcccgaggc aactttggtt
     3781 tcgaagagat actcccccgc ctggatgtcg aggttcgcgt tgtgattggc cgacgcggtc
     3841 gtcgacggga tcaacatcgc ggctacgaga ctcgccgaaa cgagcgcggc aaactttcca
     3901 accttcaaag gcactcctcc tttgttgtgg gggtgtcccg cgaaagggcc caccccggca
     3961 cctgcagcag tgtctacctt gccgttttta ggacttaatg gctagaggga ttccgaaata
     4021 tccggttcgg aggaatcaag caatccaagg acgaggccgt cgagtatgtc tctctcggaa
     4081 acgagcaggt cggttgcgcc cgcggcgtcc atcacgcaga gcaagatctc ggcccctgca
     4141 acgatcacgt cgacgcggcc ggggaccaac cctttcactt gcttgcgttg ctcgaagggc
     4201 aatatttcca accggcgcgc gagcttgcgc acgtcactat gcgtcagaac ggaatgatgc
     4261 gtgagttccg gctcgtacgc atccaaaccg agcttcaagg ccgccagttg ggtcaccgtc
     4321 ccggctacac cgatcaggcg cgttccgggt tcgacttcga atgagtgctc ggccaacac
//
LOCUS       DATMED010000291         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3307649, whole genome shotgun sequence.
ACCESSION   DATMED010000291 DATMED010000000
VERSION     DATMED010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307649"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VNP73_07440"
     CDS             complement(<1..912)
                     /locus_tag="VNP73_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWL65790.1"
                     /translation="MSKVAVVTLGCPKNAIDSEGLGGLLAAGGHEMTEGPEDADVVVV
                     NTCGFIDPARRETIEEVLDLAELKDRGLKGLVLTGCLVARSAEELEDNLPEVDALVDF
                     AAYPRISEIVSGVAAGSLETKVFGTPGTRFDPAWWDASIAAAPRIRFGRAPWSYVKIA
                     EGCDRGCTFCAIPLMRGKFRSRSAQIVTTEIAHLASQGVSEFSLVSQDSVMWGRDTGE
                     GDLSFLLERLGEVDGVERIRLMYLHPQGVTEGLIDTILASPVVVNYFDLSLQHIAPVE
                     ETDERGRPKRRAVADATGHRFHEFRVRN"
     gene            complement(917..1702)
                     /locus_tag="VNP73_07445"
     CDS             complement(917..1702)
                     /locus_tag="VNP73_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWL65791.1"
                     /translation="MLSEPSTIGSYLRAARRRRRVSIERAAEETRIRADYLMRMESDE
                     FDFLAPAYVRGFLKTYCRFLRIKWEPMLEEFDRRFGAGRFESQQIIALERHGRQHTPK
                     EPRLSRSWTAAAVVATGALLILAVVGLILGPEEEEPDRAPTVAEADDSPSVSPSPSAP
                     VPIESTTTASEDAIAFAEGIELEIVASEADCWVQAVSDGGQPVAQTLQMGDSVTFTAD
                     DELFVRLGFPEGVELIVNGTSIGSPEGSDPVNLTFPDDIDLFL"
     gene            complement(1776..>2617)
                     /locus_tag="VNP73_07450"
     CDS             complement(1776..>2617)
                     /locus_tag="VNP73_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HWL65792.1"
                     /translation="KKAASALNWVVREMEMRYETLAHSGMRNIEFYNDAAKRGEVVKR
                     TEDDPDPYPLPYILVVVDELSDLMMVAPRDVENAICRIAQMARAVGIHLVVATQRPSV
                     DVVTGVIKANIPSRMAFSVASQQDSRVILDQGGADKLIGHGDMLFLHANASKARRIQG
                     AWVNEKEIAAVVAHARRQGHPVYVEGVTADDVAPGGGGAGGGVGADDELLQEALELVV
                     RSKLGSTSMLQRKLQVGFARAGRLMDLLEQRGIVGPSHGSKPRDVLMTIEELEGAGAP
                     ADG"
BASE COUNT          480 a          865 c          779 g          493 t
ORIGIN      
        1 attcctcacg cggaactcat gaaatcggtg gcccgtcgcg tcagcgacgg cgcgtcgctt
       61 cggccgtccc cgctcgtctg tctcttccac cggtgcgatg tgttggaggg aaaggtcgaa
      121 gtagttcacg acaacaggag aagcaaggat cgtatcgatc agcccttcgg tcaccccttg
      181 agggtgcagg tacatcaacc tgatccgctc gacaccgtcg acctcgccca gccgttcgag
      241 taggaacgac agatctccct ccccggtgtc gcgtccccac atgaccgagt cctggctgac
      301 cagtgagaac tcagacactc cctgtgaggc gaggtgcgcg atctccgtgg tcacgatctg
      361 agcggagcga gaacggaact tgccgcgcat cagtgggatc gcgcagaagg tgcagcctcg
      421 atcgcaaccc tcggcgatct ttacgtacga ccacggcgcc cgtccgaagc ggatgcgcgg
      481 cgccgcggcg atcgatgcat cccaccacgc cgggtcgaac ctggtgcccg gggtgccgaa
      541 caccttcgtc tcgagggagc cggcggcaac ccccgacacg atttcggaga tccgagggta
      601 ggcggcgaaa tccaccaggg catcgacctc gggcaggttg tcctcgagtt cctcggccga
      661 cctggccacc aggcagccgg tgagaaccag gcctttgagg cctctgtcct tcagttctgc
      721 caggtcgagg acctcctcga tcgtctcccg acgggccgga tcgatgaagc cgcacgtatt
      781 gaccacgacg acgtcggcat cctctgggcc ttcggtcatc tcgtgaccac ccgcagccaa
      841 cagaccgccc aagccctcgg aatcgatggc gttcttagga cagcccagag tgacgacggc
      901 gacttttgac atccggttag aggaagagat cgatgtcgtc ggggaaagtg agattgaccg
      961 gatccgagcc ttcgggcgaa ccgatgctcg tcccgttgac gatcagttca acgccttccg
     1021 gaaaacctaa gcgcacgaac aactcgtcgt ccgcagtgaa cgtgacgctg tcccccatct
     1081 gaagcgtttg cgcgaccggc tgtccgccat ccgaaacagc ttgcacccaa cagtcggctt
     1141 ccgacgcgac tatctcgagt tcgatccctt ccgcgaacgc gatcgcgtcc tcgctcgccg
     1201 tagtggtcga ctcgatcggc acgggagcgc tgggactcgg actcacgctc gggctgtcgt
     1261 ctgcctccgc cacggtcggc gccctatcgg gttcctcttc ctcgggaccc aggatcaaac
     1321 cgacgaccgc gaggataagc aatgctccgg tggcgacgac tgcagccgcg gtccagctcc
     1381 gcgagagcct cggctcctta ggtgtgtgtt gtctgccgtg cctttcgaga gcgatgatct
     1441 gctggctctc gaaccggccg gcgccgaacc tgcggtcgaa ctcctccaac atgggttccc
     1501 acttgatgcg caggaagcgg caatacgtct tcaagaaacc gcgtacgtat gcgggcgcga
     1561 ggaagtcgaa ctcgtccgac tccatacgca tgaggtaatc ggcccggatc ctcgtttctt
     1621 ccgcggctcg ttcgatgctc acccggcgac ggcgccgggc ggctcggagg tacgacccta
     1681 tggtcgatgg ttcgctcaac aacgctccgc gctataggag atttccggac cgcgattata
     1741 ggaacggccg ggctagatgc gcgcttcgaa aggtttcagc cgtcggctgg agcgcctgcg
     1801 ccctctaatt cttcgatagt catgagcaca tcgcgcggct tggacccgtg tgagggaccc
     1861 acgattccac gttgctcgag tagatccatg agtcttcccg cgcgggcgaa cccgacctgg
     1921 agcttccgtt gaagcatcga agtcgagcca agcttggacc gaaccacgag ctcgagagcc
     1981 tcttgcagca actcgtcgtc ggcaccaact ccaccgccgg cgccacctcc accgggtgcc
     2041 acgtcatcgg cggtgacacc ctcgacgtaa acgggatggc cctgtctccg tgcgtgcgcg
     2101 acgactgccg ctatctcctt ctcgttgacc cacgccccct ggatccttcg cgccttggag
     2161 gcgttcgcgt gcaggaagag catgtcgccg tgaccgatca gtttgtcggc cccgccctga
     2221 tcgaggatca cccgcgagtc ctgctgcgag gcgacgctga atgccatgcg tgacgggatg
     2281 ttcgctttga tgacaccggt cacgacgtcg acggaaggac gttgcgtcgc gacgacgaga
     2341 tggatgccga cggcgcgcgc catctgcgcg atcctgcaga tcgcattctc gacatcgcgt
     2401 ggggccacca tcatcaggtc cgagagctca tcgacaacga ccaagatgta gggaagcgga
     2461 tagggatccg gatcgtcctc cgtgcgcttt acgacctcgc cgcgcttagc cgcatcgttg
     2521 tagaactcga tgttgcgcat ccccgaatgt gcgagcgtct cgtaacgcat ctccatctcg
     2581 cgcacgaccc agttcagcgc gcttgccgct ttcttca
//
LOCUS       DATMED010000292         5866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4072780, whole genome shotgun sequence.
ACCESSION   DATMED010000292 DATMED010000000
VERSION     DATMED010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4072780"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..378
                     /locus_tag="VNP73_07455"
     CDS             175..378
                     /locus_tag="VNP73_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWL65793.1"
                     /translation="MADGTVKWFSNEKGYGFISQSDGEDVFVHFSAIEGDGYKSLQEG
                     QAVEFEVTDGPKGKQASNVRPVA"
     gene            complement(435..935)
                     /locus_tag="VNP73_07460"
     CDS             complement(435..935)
                     /locus_tag="VNP73_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65794.1"
                     /translation="MGEEEELEQESRRPKVVDKRISAGGHAPPAKPSPEPVAAEGPAP
                     GDEPPQAPERFESPSGGTPPPPGGAEQVWTPEQEAEAKRIVEEIVRTPSLDWVLNVAV
                     NLANVAGTKLEMGAAQDAQLAIDALASLINGLGDRLQDAEAPLKQTLAQLQLAYAQRA
                     GAPADK"
     gene            1197..1847
                     /gene="infC"
                     /locus_tag="VNP73_07465"
     CDS             1197..1847
                     /gene="infC"
                     /locus_tag="VNP73_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWL65795.1"
                     /translation="MAQELRVNDRIRVPRVRVVGADGSQVGILEINEALDMAQSQDLD
                     LVEVAAQADPPVCRIMDYGKYKYDQAVKQKESRKRQSLVVVKEIKMRPKIDIHDYGTK
                     KGHVVRFLRQGAKVKVTIMFRGREMAHLDLGKRLLDRLAEDVSEIAKVEVPAKVDGRN
                     MTMVLNPLKEAITPKPAPPPKRGPAPERVETPEAEEQEEEEPAAPPQIKVTRAEGG"
     gene            1851..2045
                     /gene="rpmI"
                     /locus_tag="VNP73_07470"
     CDS             1851..2045
                     /gene="rpmI"
                     /locus_tag="VNP73_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWL65796.1"
                     /translation="MPKMKTHRGAAKRLKPLPSGKIRRRKAFASHILEKKSPRRKRRL
                     QNPALVAKADRKRVRRLLGI"
     gene            2136..2513
                     /gene="rplT"
                     /locus_tag="VNP73_07475"
     CDS             2136..2513
                     /gene="rplT"
                     /locus_tag="VNP73_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWL65797.1"
                     /translation="MPRVKRSVAGRKKRREVLKQAKGYYSSRGKHYRAAREQLLHSGT
                     YAYRDRRARKGDFRRLWITRINAAARINGLSYSKLIAGLRLAEIEVDRKIMAELAVNE
                     PEAFAALAEAAKSALESQEKKAS"
     gene            2546..3307
                     /locus_tag="VNP73_07480"
     CDS             2546..3307
                     /locus_tag="VNP73_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWL65798.1"
                     /translation="MLAARKLTRRAHRDKSGTFLLEGPRNVGEALAGSGRVIEVFVDE
                     PSKHADVIERAERAGVPVRSVTGPVMKALADASTPQGVVAVARIEPLTMDDLGGASLV
                     LVLDQVRDPGNAGTLVRSAVAAGADGVVFTTGSVDPFGPKTSRAAAGTVLKTKIVRDV
                     ALESAASSLSGDGLVVVGADAKAETRADGCNLTRRLALVIGNESWGLTPESRALMDET
                     VAIPMPGPAESLNAGIAGSILLFECVRQRSPRTPG"
     gene            3399..4100
                     /locus_tag="VNP73_07485"
     CDS             3399..4100
                     /locus_tag="VNP73_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWL65799.1"
                     /translation="MSEPSQEAPRFAEMISKIAHELRSPLTSVKGFSATLLKRWDRFT
                     DEQKLQFIETIHFDAERMGRIVSEVLDLARLETGKLELHPTLVDLHAIATQAAEHHKT
                     LPGADRVVVEVEEGTRAWGDPDRLAHVVGNLIENGVKFSDEGPIEVTARRANGMVELT
                     VTDQGIGIEADLLPTVFDGPAPAGGKATPSGTGLGLYLSRRLVEAHGGTITVESRSGE
                     GATFVVSIPAESPDD"
     gene            4093..5178
                     /gene="pheS"
                     /locus_tag="VNP73_07490"
     CDS             4093..5178
                     /gene="pheS"
                     /locus_tag="VNP73_07490"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWL65800.1"
                     /translation="MTDDIRAAFESAGTEGLTRISEATDLASLEEAKIRILGRKSALS
                     RARAGLGSLAPEEKRDIGKLANEVQARLEAAIEERRQAFEAVELEQRWERERLDVTLP
                     GHAPHIDPVHPLTRTIWEIVDIFIGLGYKVAEGPEAELSSYNFDALNAPPAHPSRSPL
                     DTFYLEGTNEEVCLRPQTSPMQMRTLEAQPPPVYVVVPGRCYRRDTVDATHLNGFTQL
                     EGLAVDEGITMGDLKGTLQAFAREIFGKGLDTRLRPHYFPFTEPSAELDVQCFKCRGD
                     GCSFCKQEGWIEVLGCGMVHPFLLEWARDHTADDSIRAAYASERYTGFAFGMGIERIA
                     ALAHGVSDIRYFWENDLRFLEQFRAIS"
     gene            5181..>5866
                     /locus_tag="VNP73_07495"
     CDS             5181..>5866
                     /locus_tag="VNP73_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWL65801.1"
                     /translation="MSLKWLREYVDVDTQPEKLAEMLDLSGTKVEAVHRLGEGIEGVV
                     VAEVLEIEEHPDADNLVLVDVKTTESDVAHVVCGARNFKVGDKVPFARVGARLPGLEI
                     GERKIRGRPSQGMLCSAAELGVSKDHSGILVLPPDSPLDREVVELLDLEDVVFELEIT
                     SNRPDCMSVIGVAREVSAVLGTELRMPEVSVPAAGISTGVNVTIKDADGCPRYLARFI
                     EGVKVGSSPA"
BASE COUNT         1142 a         1716 c         1982 g         1026 t
ORIGIN      
        1 gggagcagag gacatcatcg aacttcccgt tacaaagatc gtgccctaga ttcgtcacag
       61 atgcccataa ctcggtagat ttcgtgtgct agcgttcggg gcgcttcagt acaggggccg
      121 acacttgcag ggacaaaggc ctgcgggccc ttgtagcgga aagggagtgg agtagtggct
      181 gacggaacgg tgaagtggtt cagcaacgag aaaggctatg gcttcatctc gcaatcagac
      241 ggtgaggacg tcttcgtcca cttcagtgcc atcgaaggag atggctacaa gtcattgcaa
      301 gagggccagg ccgtcgagtt cgaggtaacg gacggtccga agggcaagca ggcgtcgaac
      361 gtaaggccgg tcgcctaaga cacttcccac atcaggctcg aaatgaaaaa gcggccccct
      421 tcggggggcc gcagtcattt gtcagccgga gcccccgctc gctgagcgta cgccagttga
      481 agctgggcga gcgtttgctt gagaggcgcc tccgcatcct gcagccgatc acccagtccg
      541 ttgatgagac ttgccaacgc gtcgatcgcc aactgcgcgt cctgcgcagc ccccatctcc
      601 aacttcgtcc ccgcaacatt ggccaggttc acggccacgt ttaggaccca gtcgagactg
      661 ggcgtacgaa cgatctcctc gacgatccgt ttggcttcag cctcctgttc gggagtccac
      721 acctgctcgg ctccacccgg cggaggcggc gtgccgccgg agggcgactc aaaacgctct
      781 ggagcctgag gcggttcgtc gccgggagcc gggccctcag ccgcaaccgg ttcaggcgac
      841 ggctttgccg gcggcgcgtg gcccccggcc gagatgcgtt tgtcgacgac cttgggccga
      901 cgcgactctt gctcgagctc ctcttcttca cccatggttg gtagatatac acttcttgga
      961 ccgttgaaac gtcaagaagg ggatgacccc tcacctgccg gccgatgaag ttacaggtcg
     1021 tgtgggtcgg acattcaccg tttagccggt gatgccgttc ttggcgtcgc cggttttttg
     1081 ttgtctggtg gtctcgacca ccagctgaac agaatggccg gcgagggtcg gttcgtcgaa
     1141 cgccgacggg ctcggccgtt ggcgggaaga gatgtcggac gagtaaggag aagcggatcg
     1201 ctcaagagct gcgagtgaac gatcgcatcc gtgtgcctcg ggtgcgtgtg gtcggggccg
     1261 atgggtctca ggtagggatc ctcgagatca acgaggccct cgatatggcc cagagccaag
     1321 acctcgatct cgtcgaagta gcggcccagg cagacccacc ggtctgccgg atcatggact
     1381 acggcaagta caagtacgac caggccgtga agcagaaaga gtcacgcaag aggcaatcgc
     1441 tggtggtcgt gaaagagatc aagatgcgac cgaagatcga catccacgac tacgggacca
     1501 agaaggggca cgtggttcgg ttccttcgcc agggggcgaa ggtgaaggtc acgatcatgt
     1561 tccgcgggcg ggagatggcg catctcgatc tggggaagcg gctactcgat cgcctcgcgg
     1621 aggacgtgtc ggagatcgcc aaagtggaag tgcccgcgaa ggtggacggc aggaacatga
     1681 cgatggtgtt gaaccctctc aaggaggcga tcacaccgaa gccggctcct ccgcccaagc
     1741 gggggccggc gccggagcgc gtagagacgc cggaagcgga agaacaagaa gaagaagagc
     1801 ccgcggcgcc gccgcagatc aaggtgactc gggcagaggg aggctgattc atgccgaaga
     1861 tgaaaacgca tagaggagcg gcgaagaggt tgaagcctct tccctccgga aagattcgcc
     1921 gcaggaaagc gttcgcgagc cacattctcg agaagaagtc gcctcgtcgt aagcgccggc
     1981 tacagaaccc ggcgcttgtc gctaaagccg atcggaagcg cgtacgcagg ctgcttggca
     2041 tctaacgaac acgtactcat cagcgatcgc ctcggcgatc gcgaagcgaa cagcgatcgc
     2101 ctcggcgatc gcgaagcgaa acgggaggga tatccatgcc cagggtcaag cgcagcgtcg
     2161 ccggaaggaa gaagcgtcgc gaagttctga agcaagcgaa gggttactac tcgtcgcgcg
     2221 gcaagcacta ccgcgccgcg cgagagcaac tgctccactc cggcacctat gcatacaggg
     2281 ataggcgggc ccgcaagggt gatttccgcc ggctgtggat cacccgcatc aacgctgccg
     2341 cccgcatcaa tgggctctca tacagcaagc tcatcgccgg tctgcgtctc gccgagatag
     2401 aggtggatcg caagatcatg gcggaactgg cggtgaacga gccggaggct ttcgcagctc
     2461 ttgccgaggc tgcgaagagc gcactcgaga gccaagagaa aaaggcgtca tagaaggccc
     2521 gatctccagc cttcagaacc cgcgcgtgct cgccgcgcgc aaactgaccc gtcgcgctca
     2581 cagggacaag agcggcactt ttttgttaga gggcccgcgg aatgttggag aggcgctcgc
     2641 cggcagtggt cgcgtgatcg aggtattcgt cgatgagccg tcaaagcacg ccgacgtgat
     2701 cgagcgggcc gaacgtgcgg gcgttccggt tcgctccgtg accggcccgg tgatgaaggc
     2761 gctggccgac gcgtcgacac ctcagggcgt ggtcgccgtc gcccggatcg agccactcac
     2821 gatggacgac ttggggggag cgagtctcgt gctggtcctc gaccaggtca gggatcccgg
     2881 caatgccgga accctcgtgc gatcggccgt ggccgcggga gccgacgggg tcgtcttcac
     2941 aacaggctcg gtggaccctt tcggccccaa gacgtcccgg gcggcggccg gcaccgtctt
     3001 gaagaccaag atcgtcagag acgtagcact ggagagtgcc gcgtcctctc tgtcgggaga
     3061 tgggctcgtc gtggtgggcg ccgatgcgaa ggccgagacg cgtgccgacg gatgcaacct
     3121 cacccggcgt ctcgctcttg ttatcggtaa cgaatcgtgg gggctgactc cggagagtcg
     3181 agccttgatg gacgagacag tagcgatccc gatgccgggt cctgccgaat ctctcaatgc
     3241 cggtatcgcc gggtcgatcc tcctgttcga gtgcgttcgg caacgaagcc cgagaacgcc
     3301 cggatagccc gtttgctctg gccgaacgtc gagacgttcg gcttgggctg ctctggccga
     3361 acgtcgagac gttcggcttg gctctatcct ctgccgttat gagtgaaccc tcacaggaag
     3421 ccccgcgttt cgccgagatg atctcgaaga tcgcccacga gctgcgttcg ccgttgacct
     3481 cggtaaaggg gttctcggcc acgctcctga agcgttggga caggttcacc gacgagcaga
     3541 agcttcaatt catcgagacg atccacttcg atgccgagcg gatgggccgc atcgtttccg
     3601 aggtgctgga tctggctcgt ctcgaaaccg ggaaactcga actccatccc acgctggtgg
     3661 atcttcatgc gatcgcgact caggcggccg aacatcacaa gacccttccg ggtgccgaca
     3721 gggtcgtggt tgaggtcgaa gaaggaactc gggcgtgggg agatccggat cgcctcgcgc
     3781 atgtcgtcgg taacctgatc gagaacggcg tcaagttctc ggatgagggt ccgatcgagg
     3841 taacggcgcg ccgggctaac gggatggtcg aactcacggt cacggatcaa ggcatcggga
     3901 tcgaagcaga ccttctgccg acggtgttcg acggaccggc tcccgcgggg ggcaaggcga
     3961 cgccgtcggg gacgggattg ggtctgtacc tgtcgcgccg gttggtcgaa gcacacggcg
     4021 gaacgatcac ggtcgagagc cgctccggcg agggtgcaac gttcgtggtc tcgatcccgg
     4081 ccgaaagtcc cgatgactga cgacatcaga gccgctttcg agagcgcagg gaccgaagga
     4141 ctcacgcgga tctcggaggc aaccgacctg gcctctctcg aagaggcgaa gatccggatc
     4201 ctggggcgca agtcggcctt atctcgagcg cgcgccggct tgggctcgct ggcccccgaa
     4261 gagaagcgtg atatcgggaa gctcgcgaac gaggttcagg cccggctcga agcggcgatc
     4321 gaggaacgca ggcaggcatt cgaggcggtc gagctcgagc aacggtggga gcgggaacgg
     4381 ctcgacgtga cgttgccggg gcatgcccct cacatcgacc cggtccaccc tctgacgaga
     4441 acgatctggg agatcgtcga catcttcatc ggtcttggat acaaggttgc ggaagggccc
     4501 gaggccgagc tctcgtccta caacttcgat gctctgaatg cgccgcccgc gcacccctcg
     4561 cggtcgcctc tcgacacctt ctatctagag gggacgaacg aggaggtgtg tctgcggccc
     4621 caaacctctc ccatgcagat gcgcacgctg gaagctcagc ctccgcccgt ctacgtggtt
     4681 gtcccggggc gttgttaccg gcgcgacacg gtcgacgcaa cgcacctgaa cgggttcacg
     4741 caactggagg gtctcgcggt ggacgagggg atcaccatgg gggacctgaa gggcacgctc
     4801 caggcgttcg cgcgcgagat cttcggaaag ggtctcgaca cgcgcctgag gccgcactac
     4861 ttcccgttca cggagccgtc ggccgagttg gacgttcaat gcttcaaatg tcggggcgac
     4921 ggatgcagct tctgcaagca ggaggggtgg atcgaggtgc tgggatgcgg gatggtccat
     4981 ccgttcctgc tcgagtgggc acgcgatcac actgcggacg acagcatccg ggcggcctat
     5041 gcgtcggagc gttacacggg cttcgcgttc gggatgggga tcgagcggat cgcggccctc
     5101 gcgcacggcg ttagcgacat ccgttacttc tgggagaacg atcttcgttt cctcgagcaa
     5161 ttccgggcca tctcatgaga gtgagcctga aatggttgcg cgagtacgtc gatgtcgaca
     5221 cgcagcccga gaagctcgcc gagatgttgg acctgtccgg caccaaggtc gaggcggttc
     5281 atcggctcgg tgaaggtatc gagggtgtgg tcgtggccga ggtgctcgag atcgaggagc
     5341 acccggacgc ggacaacctc gtgctcgtag acgtcaagac gaccgaatcc gacgtagctc
     5401 acgtcgtctg cggcgcgcgc aacttcaagg tgggggacaa ggtcccgttc gcgcgcgtgg
     5461 gggcccggct accgggtctc gagataggcg agcgcaagat caggggccga ccaagtcaag
     5521 ggatgctgtg ctccgcggcg gagttgggcg tgtccaagga tcactcgggc atcctcgtgc
     5581 ttccgcccga cagccccttg gatcgcgagg tcgtcgagct cctggatctc gaggatgtcg
     5641 tgtttgagct cgagatcacc tcgaaccgcc ccgattgcat gagcgtcatc ggagtggctc
     5701 gagaggtatc ggcggttctg ggcacggagt tgcggatgcc ggaagtatcg gtcccggccg
     5761 cggggatctc gaccggggtg aacgtgacga tcaaggacgc ggatggctgt cctcgttatc
     5821 tcgcccggtt catagagggc gtcaaggtcg gttcgtcgcc ggcatg
//
LOCUS       DATMED010000293         2764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4830938, whole genome shotgun sequence.
ACCESSION   DATMED010000293 DATMED010000000
VERSION     DATMED010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4830938"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VNP73_07500"
     CDS             <1..1212
                     /locus_tag="VNP73_07500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65802.1"
                     /translation="WLVVEGNPEFFRITKRIHNHLHGFPGDGGPLGPQEHSVYLDALQ
                     PNAEALARVVKPRDIVILHDPQTAGLVAPMKEVADIVVWRCHVGLDTPNDIAHNAWDF
                     LEEHVEMADAHVFSRQAFVWLGLDPEKTVIIPPSIDAFSPKNQELGRPIVDGILTASG
                     LIEGGDPGLATFSRAGGEPGLVKRSAELVDGGPPLAADARIVTQVSRWDRLKDPLGVM
                     KGFADHVATTSDAHLVLAGPAVEAVSDDPEGAQVLEEVRVAHSQMSPEIRARIHLAAL
                     PMADSEENAAIVNALQRRSEVVVQKSLAEGFGLTVAEAMWKARPVVASRIGGIQDQIV
                     DGVSGSLVRDPGDLAEYGNAVTKLLADSERAEQMGIEAMDRVRTEFLGPRHLLQYLSL
                     LSKLMQTSAAR"
     gene            complement(1209..1772)
                     /locus_tag="VNP73_07505"
     CDS             complement(1209..1772)
                     /locus_tag="VNP73_07505"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWL65803.1"
                     /translation="MRFDAVVLAGGDARRLGGLDKAAIEVGGVSLLDRALDAIQGAVE
                     VIVVGPRRPTSAVVRWTSESPPGGGPLRATVAGLEHARSDVVVVLAVDYPFVTRAVVA
                     ELIRATGTHDGAALQDEGGAMHYVVGAYRTDALRSALEAHPPVQASMRSLFATLDVAV
                     VHADRAALDVDTPEDLESARASDSEVD"
     gene            1926..>2764
                     /gene="hemE"
                     /locus_tag="VNP73_07510"
     CDS             1926..>2764
                     /gene="hemE"
                     /locus_tag="VNP73_07510"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWL65804.1"
                     /translation="MNHRESNFIRACKGEPTEHTPIWFMRQAGRAFAEYRKLREKHSI
                     LELCRTPDLAAEVTLQPVDRLGVDAAILFSDIVVPLLAMGVDVDIVEGVGPVIENPVR
                     SAQDVGRLVPLEAEEGVPHVIESVRILVDELKVPLIGFGGAPFTLASYMIEGRPSKDH
                     ALTKSFMYSQPEAWVRLMDLLADSILEYLRAQIAAGASAVQLFDSWAGALDADDYNAH
                     VAPAVGKIFTGLSDEDVPRILFGVTTGEILGDMAATGPDVMGVDWRVPLETARARMPQ
                     SVTA"
BASE COUNT          517 a          901 c          860 g          486 t
ORIGIN      
        1 tggctcgtgg tcgagggaaa tcccgagttc ttccggataa caaagcgaat acacaatcac
       61 ctgcacgggt ttcccggtga cggcggcccg ctcggccccc aggaacattc ggtctacctc
      121 gatgccctgc aacccaacgc cgaggctctt gctcgggtcg ttaaacccag ggacatcgtc
      181 atcctgcatg acccacagac agctggtctc gtcgctccga tgaaagaggt ggccgatatc
      241 gtggtctggc gttgccatgt cggcctcgat acccccaacg acatcgcgca caatgcctgg
      301 gactttctcg aggagcacgt ggaaatggcc gacgcccacg tcttctcccg gcaggcgttc
      361 gtatggctgg gcctcgaccc ggagaaaacg gtaatcatcc cgccttcgat cgacgccttc
      421 tcacccaaga accaagagct cggccggccc atagtcgacg gaatactgac cgcctctgga
      481 ctgatagagg gtggcgaccc tgggttggcc acgttctcca gagccggggg tgagccgggt
      541 ctcgtcaagc gatccgccga gctcgtggac ggaggtcctc cactcgcggc cgacgcccgt
      601 atcgtcaccc aggtgtcgcg gtgggatcgc ctcaaggatc cccttggcgt catgaagggg
      661 ttcgcggatc acgttgcaac cacgagcgac gcccatctcg ttctggcggg cccggccgtc
      721 gaagcggtca gcgatgatcc cgagggggcc caggtgctcg aggaagtgcg cgtcgctcac
      781 tcgcagatgt cacccgagat tcgggcccgc atccaccttg cggctctgcc catggccgac
      841 agcgaggaaa acgctgccat cgtcaacgct ttgcagagac gttctgaagt cgttgttcaa
      901 aagagtcttg cggagggctt cgggctaacg gtcgctgaag ccatgtggaa ggcacgtcct
      961 gtggtcgcaa gccgcatagg agggatccag gaccagatcg tcgacggagt aagcggttcg
     1021 ttggtgcgcg acccaggcga tctcgccgaa tacgggaacg cggtcacgaa gctcctagcc
     1081 gactccgagc gagcggaaca gatgggcatc gaggcgatgg atcgcgtgcg caccgagttc
     1141 cttggcccta ggcacctgct ccaatacttg agcctgttgt ccaagctgat gcagacatcg
     1201 gccgcgcgct agtcaacttc tgagtcagac gctcgcgcac tctcgaggtc ctcgggtgtg
     1261 tcgacatcca gagccgcacg atcggcgtgc acgacggcga cgtcgagagt cgcgaacaag
     1321 gaccgcatcg atgcctgaac tggagggtgt gcctcgaggg cggagcgcaa cgcgtccgtt
     1381 cgatacgcgc ccaccacgta gtgcatggca ccaccctcgt cctgaagcgc agctccatca
     1441 tgcgtcccgg ttgcacgaat cagctcggca acgaccgcgc gggtgacgaa gggatagtcg
     1501 accgcgagga ccaccaccac gtcacttctt gcgtgctcga gaccggcgac tgtcgcccgc
     1561 agcggtccac cccccggtgg gctctcggag gtccatcgca cgactgccga agtcgggcga
     1621 cgcggcccca cgacgattac ctccactgcc ccttggatcg cgtcgagggc ccgatcgagg
     1681 agggacaccc ccccgacctc gatcgccgcc ttgtccaggc caccgagtct gcgggcgtct
     1741 ccacccgcca gcacgaccgc atcgaacctc atcaccgcac cctatgaggc gaaacccctc
     1801 tgggcttcgg cgaagcttcg ctgcgcctcg ccgagggaaa aggctcggca cccgccctgc
     1861 gggcggtcaa gtcgccgctt taggatgcgg caatcggtgc tgccgaaacc attgatcgga
     1921 gccgcttgaa tcaccgcgag tccaacttca tccgcgcatg caagggcgag ccaaccgagc
     1981 acacgcccat ctggttcatg cgtcaagcgg gccgggcgtt cgcggaatat cgcaaactgc
     2041 gcgagaagca ctcaatcctc gagttgtgtc gcaccccaga cctcgcagca gaggtgacgt
     2101 tgcagccggt cgaccgactc ggcgtggatg ctgcgattct gttctccgac atcgtcgtcc
     2161 ccttgctcgc aatgggggtg gacgtcgaca tcgtggaggg ggtcggaccg gtcatcgaaa
     2221 atccggttcg atccgctcaa gacgtcggcc gtctcgttcc actcgaagcc gaagaaggcg
     2281 taccgcacgt tatcgagtcg gtgcgaatcc ttgtcgacga gctgaaggtt ccgttgatcg
     2341 ggttcggcgg ggcacccttc acactcgcca gctacatgat cgagggcaga ccaagcaagg
     2401 atcacgctct cactaagtcg ttcatgtact cgcagccgga agcgtgggtt cgactgatgg
     2461 acttgctggc ggattcgatc ctcgaatatc tgcgcgcgca gatcgcagca ggcgcgtccg
     2521 ctgtgcaact cttcgactca tgggccggcg cgctcgacgc cgacgattac aacgcccacg
     2581 tggcgccggc ggtcggaaag atcttcacgg gtctctcgga cgaggacgtc ccgcgcatct
     2641 tgttcggcgt gactacgggc gagatccttg gcgacatggc cgcgaccggg cccgacgtga
     2701 tgggtgtgga ctggcgcgtg cctctcgaga cggcgagagc ccgaatgcca cagtcggtca
     2761 ccgc
//
LOCUS       DATMED010000294         5354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3311304, whole genome shotgun sequence.
ACCESSION   DATMED010000294 DATMED010000000
VERSION     DATMED010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3311304"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VNP73_07515"
     CDS             <1..832
                     /locus_tag="VNP73_07515"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65805.1"
                     /translation="PAWLWYVGFFVAPLVIMVAYSFAQNDPKEFFTVTFGFYTEQFQR
                     VWDPIYLSIYRNTVIMAFLGTVGCLLIGYPFAYFLATRAGKHKTLLFLLVIVPFWTSL
                     LIRTYAWVLILGERGPLSDGLEWVGLLDRPLDVLYTPGAVLVGVIYDYLPLMVFPLYV
                     AIERMDVRLKEASRDLGAGRWSTFWRVTMPLTAPGILTGCLLTFIPMMGEYVVPEILG
                     GAKSYLIGNLIANEFRTAIDWPFGAALGMTLVVLMLIVIFVYLRVLGRRAEENLGAAM
                     "
     gene            829..1677
                     /locus_tag="VNP73_07520"
     CDS             829..1677
                     /locus_tag="VNP73_07520"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL65806.1"
                     /translation="MSASGTGFARTVRTTRPLPNAPDRSVTTLLRAHAGLVYLFLYLP
                     IVVVVIYAFNDSRLVQIWHGFSTRWFGEAWRDDTLRNGLRISLITASLNAVLATVLGT
                     SAGIALRNVGRKTRTSFDMFMYTTLIVPEIVIAIASLLFFVTLGLPLGPIAIVITHSV
                     FNTSVVALIVRARLAGMDRSLEEASRDLGASKWGTFRQVTLPQLFPAVLAGALLAFTF
                     SFDDVVLSSFVSGVGSTTLPVEIFSQIRFGLSPKVNVVAVVTLSMTFSAIVAAQILLR
                     REARRA"
     gene            1725..2141
                     /locus_tag="VNP73_07525"
     CDS             1725..2141
                     /locus_tag="VNP73_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65807.1"
                     /translation="MTRERWNRIALWLFFAGGIAWGLKLIAIAANGGEGSPLEGILFI
                     IGLATLLVGSSGIGGTLLLRAARPVYLAGCILSPVAFWFFFTFMDPVGRSVLSPPEDH
                     WFYDETGILITAVTAAVVGLLLLRRKGNIEPGKGLP"
     gene            2227..2430
                     /locus_tag="VNP73_07530"
     CDS             2227..2430
                     /locus_tag="VNP73_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWL65808.1"
                     /translation="MATGTVKWFNEAKGYGFITPEDGSKDLFVHFSNIQAEGYKTLTE
                     GATVSYEARQGQKGPEATNVVPT"
     gene            2513..4348
                     /gene="typA"
                     /locus_tag="VNP73_07535"
     CDS             2513..4348
                     /gene="typA"
                     /locus_tag="VNP73_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWL65809.1"
                     /translation="MSVRDDLRNVAIVAHVDHGKTTLVDAMLWQSGAFRSNQQVNERV
                     MDSMDLEREKGITILAKNTAVRYGDVKINIIDTPGHADFGGEVERGLTMVDGVLLLVD
                     ASEGPLPQTRFVLRKALEARLPVILVVNKVDRPDARVQEVVNEVYELFLDLDADEGQI
                     DFPILYANARAGWASLDPEVEGSDLKPLFELLLEHIPAPTYTEGHPLQALVTNLDAST
                     YVGRIALCRVFHGSIKRGEPVAWCRADETIETVKITELYVTEALDRVDADEAGPGEII
                     AIAGLADVTIGETLSDPDDPRPLPVSHFDDPSLSMTVGVNTAPLSGQDGDKVTARLIK
                     NRLDQELVGNVSIRVLDTDRPDAWEVQGRGELQLAVLVEMMRREGFELTVGKPQVVTK
                     TIDGKLHEPVERLTIDAPEEFMGVVTQLIALRKGRLENMVNHGTGWVRMEYLVPARGL
                     IGFRTEFLTETRGTGLLHHVFDRYEPWHGEVRTRPTGSLVADRKGPTTSYALLNLQDR
                     GNMFVPPGDEVYEGMIVGENSRTEDMDVNPTKEKKLTNMRSSTSDELVRLIPHKHLSL
                     EQALEFIKEDECVEVTPHTVRLRKVILDGHGRVKVSRRDAKAKVD"
     gene            4348..5121
                     /locus_tag="VNP73_07540"
     CDS             4348..5121
                     /locus_tag="VNP73_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWL65810.1"
                     /translation="MEPVYRPVIGLALTLFKTMGWQVALNGLENIPIEGPAVIATNHV
                     GYLDFVFVGYAAHKRKRLVRFMAKKEVFEHPVAGPLMRGMKHIPADRFGKAELAIERS
                     IEALKNGEIVGMFPEGTISRSFIPRTGKTGAVRIAMEAGAPLIPGVVWGTQRILTKGR
                     PKNFERKVAMSVDIDRPLPYEPVDDPTTVTERLMTRITEMVGAAERNYPQNPAVPSGE
                     FWLPAHMGGGAPTLEEADALAAKEKAERAARSAARKAQA"
BASE COUNT          992 a         1594 c         1717 g         1051 t
ORIGIN      
        1 gcccgcgtgg ctctggtacg tgggcttctt cgttgcgccg ctggtgatca tggtggcgta
       61 ctcgttcgcc cagaacgacc ccaaggagtt cttcaccgtt acgttcggtt tttacacgga
      121 acagttccag cgcgtgtggg atccgatcta tctgtcgatc tatcgcaaca cggtcatcat
      181 ggcgttcctc gggacggtgg gttgcctgtt gatcgggtat ccgttcgcct acttcctcgc
      241 gacgagggcc gggaaacaca agacgctcct gttcttgctc gtgatcgtgc cgttctggac
      301 gagtctcttg atccgtacgt acgcgtgggt gttgatcctg ggagagagag ggccgctgtc
      361 cgacggcctg gagtgggtcg ggttactcga taggccgctc gacgtcctct acacacccgg
      421 cgcggtgctg gtaggtgtca tctacgacta cctgccgttg atggtgttcc cgctttacgt
      481 cgccatcgag cggatggacg tccgtctgaa agaagcgagc cgggatctgg gggccggtcg
      541 ctggtcgacc ttctggaggg taacgatgcc gctcaccgct ccgggcatcc tgaccggttg
      601 cttgttgacg ttcatcccga tgatgggtga gtacgtcgtg cccgagatcc tcggtggtgc
      661 gaagagctat ctgatcggca acctcatcgc gaacgagttc agaaccgcga tcgactggcc
      721 cttcggagcg gcattgggga tgactctggt cgtattgatg ctgatcgtga tcttcgtgta
      781 cctcagggtc ctcggtcgtc gggcggaaga gaacctcggg gcggcgatgt gagcgcgagc
      841 ggaacgggct tcgcccgtac agtcaggacc acccgcccgc tgcccaacgc accagatcgc
      901 tcggtcacga cgctacttag ggcccatgcc ggccttgtct acctgttcct gtacctaccg
      961 atcgtggtcg tcgtgatcta tgcgttcaac gattctcgtc tcgtccagat ctggcacgga
     1021 ttctcgacgc gctggttcgg agaggcgtgg cgggacgaca ccctgcgcaa cgggttgagg
     1081 atctctctga tcacggcgag cctgaatgcc gtccttgcaa ccgttttggg gacatcggcg
     1141 ggtatcgctc tgaggaacgt cggcaggaag acccgcacct cgttcgacat gttcatgtac
     1201 acgaccttga tcgttcccga gatagtgatc gccatcgcgt cgttgttgtt cttcgtcacg
     1261 ctcgggttgc ctttgggccc catcgcgatc gtgatcacgc actcggtctt caacacttcg
     1321 gtggtggcat tgatcgtgag ggctcggctg gcgggtatgg atcgttcgct ggaagaagcg
     1381 tcgcgcgact tgggggcgtc gaagtggggg actttcagac aggtcacgct gccgcaactg
     1441 ttcccggcgg ttctcgcggg cgcgttgcta gcgttcacgt tctcgttcga cgacgtcgtg
     1501 ttgtcgtcgt tcgtttcggg agtgggctcg accacgttgc cggtcgagat cttcagccag
     1561 atccgcttcg gtttgtcgcc gaaggtgaac gtcgtagcgg tggtgacgtt gtcgatgacg
     1621 ttctccgcga tagtcgcggc ccagatcctc ttgagaagag aagcaagacg agcctagggg
     1681 tcattagggg tcagacctcg tccgggcggg taagacgatc ctccatgaca cgagaacgct
     1741 ggaatcggat cgccctgtgg ctctttttcg ccggaggcat cgcctggggc ctgaagctga
     1801 tcgcgatcgc tgccaatgga ggcgagggca gtcccctcga agggatcctg ttcattatcg
     1861 gactggccac gttgctggtg ggctcgtccg ggatcggtgg cacgctgctg ctacgggcgg
     1921 cccgccccgt ctacctcgcc ggctgcatcc tgtcccctgt cgctttctgg ttcttcttca
     1981 ctttcatgga tccggtaggg aggagcgttc tgagcccacc cgaggaccat tggttctacg
     2041 acgagacggg aatcctgatc acggcggtca ccgcagccgt ggtcggcctg ctgctgttgc
     2101 ggcgcaaggg gaatatcgag cccggaaaag ggttaccctg agcgtaagta gaacgtgagc
     2161 gtgcgttaag tgctgggcgc tgccctccct tagctcgttc ccgaaagatg cagggaggtg
     2221 tttttcatgg caaccggtac cgttaagtgg ttcaacgaag ccaaaggtta tggcttcatc
     2281 accccggaag atggttcgaa ggatctcttc gtccatttct ccaacatcca ggccgagggg
     2341 tacaagaccc tgaccgaggg tgcgaccgtg tcctacgagg cccgtcaggg tcaaaaggga
     2401 ccagaggcga ccaacgtcgt ccctacataa cgatcactag atcgatttga ggaggagggg
     2461 gcttcggccc cctctttcgc gcccacacat aaagaaggag actgcaagag agatgtccgt
     2521 aagagatgac cttcgcaacg tggcgatcgt cgctcacgtc gaccacggca agacgacgct
     2581 cgtcgacgcc atgctgtggc agtcgggcgc gttccgctcg aaccagcagg tgaacgaacg
     2641 cgtcatggac tcgatggatc tcgagcgcga gaagggcatc accatcctcg ccaagaacac
     2701 cgccgttcgc tacggcgacg tgaagatcaa catcatcgac accccggggc acgccgattt
     2761 cggcggagag gtcgaacgtg gactcacgat ggtcgatggt gtcctgctgc tcgtcgatgc
     2821 gagcgaaggc cctctgccgc aaacccgttt cgtgctccgc aaggctctcg aagctcggtt
     2881 gcccgtgatc ctcgtggtga acaaggtgga tcgtcccgat gctcgcgtgc aagaagtggt
     2941 caacgaggtc tacgagctgt tcctcgacct cgacgccgac gaggggcaga tcgacttccc
     3001 gatcctttac gcgaacgcgc gcgccggatg ggcatcgctc gatccggaag tcgagggcag
     3061 tgacctcaaa ccgctgttcg aactgctgct cgagcacatc ccggccccca cctacacaga
     3121 gggtcatccg ctgcaggccc tcgtgacgaa cctggacgcg tcgacatacg tcgggcggat
     3181 cgctctgtgc cgcgtcttcc acggatcgat caagcgcggc gagcccgttg cctggtgtcg
     3241 cgccgacgag acgatcgaga ccgtgaagat caccgagctc tacgtgacgg aggcgttgga
     3301 ccgggtggat gcagacgaag ccggcccggg cgagatcatc gcgatcgcag gacttgccga
     3361 tgtgaccatc ggcgagaccc tgtccgatcc cgacgacccc cgcccccttc cggtcagtca
     3421 cttcgacgac cccagcctgt cgatgacggt cggggtgaat accgcaccgc tgtccggcca
     3481 ggacggcgac aaggtgaccg cccggctcat aaagaaccgg cttgaccagg aactggttgg
     3541 caacgtctcg atcagggtgc tcgataccga ccgccccgat gcatgggagg tccaagggcg
     3601 aggcgagcta cagctcgcgg tgctcgtcga gatgatgcgc cgggaaggat tcgagctcac
     3661 ggtcgggaag cctcaagtgg tcacaaagac gatagacggc aagctccacg agcccgtcga
     3721 gcgcctgacg atcgatgcac ctgaggagtt catgggtgtc gtaacgcagc tcatcgcgtt
     3781 gaggaagggt cggctagaga acatggtcaa ccacgggacc ggctgggtgc gaatggagta
     3841 tctggttccc gcccggggtc tgatcggttt ccggactgag ttcctcaccg agacgcgtgg
     3901 caccggcctc cttcaccacg ttttcgatcg ctacgagccg tggcacggcg aagtcaggac
     3961 gcgaccgacg ggcagcctgg tcgcggaccg caaaggtccg acgacttcct acgccctact
     4021 taaccttcag gatcgcggga acatgttcgt gcccccgggg gatgaggtct acgaagggat
     4081 gatcgtgggc gagaactctc gcaccgaaga tatggacgtg aaccccacga aggagaagaa
     4141 gctgacgaac atgcgctctt cgacgtcgga tgagctcgtt cgcctgatcc cgcacaagca
     4201 cctgtcgctg gagcaggccc tcgaattcat caaggaggac gagtgcgttg aggtcacacc
     4261 gcacacggtt cgcctccgca aagtcatcct cgatgggcac gggcgcgtga aagtatcccg
     4321 gcgcgacgcg aaggctaagg tcgactgatg gagcccgttt accgcccggt catcggcctt
     4381 gccctcacgc tcttcaagac catggggtgg caggtcgcgc tgaatggtct cgagaacatc
     4441 ccaatcgaag ggcccgcggt gatcgcgacg aaccatgtgg gctatctaga tttcgtgttc
     4501 gtcggttacg cggcgcacaa gcggaaacga ctcgtgcgct tcatggccaa gaaagaggtt
     4561 ttcgagcacc cggtcgcggg gccgttgatg cgagggatga agcacatccc ggccgaccgc
     4621 ttcgggaaag ccgagcttgc gatcgaacga tcgatcgagg cactcaagaa cggcgagatc
     4681 gtgggcatgt tccccgaagg gacgatcagc cgctcgttta tcccccgaac cggcaagacc
     4741 ggcgcagttc gcatcgcgat ggaagcggga gcaccgctca tccccggtgt cgtgtgggga
     4801 acgcaacgga tcctcacgaa gggccggccc aagaacttcg agcgcaaggt cgcgatgagc
     4861 gtcgacatcg accgaccgct gccttacgag ccggtggacg atcccacgac cgtgacggag
     4921 cgactgatga cgcggatcac cgagatggtg ggcgcggcgg agcgcaacta tccacagaac
     4981 ccggccgttc cgtcgggcga gttctggctt ccggctcata tgggcggggg agcgccgacc
     5041 ctggaagagg cggacgcgct ggcggcgaaa gagaaggccg agcgagcggc gcgctccgcg
     5101 gctcgaaagg cacaggcata gatagggttc ggaccggcgt cggtgtcgta gcgcctctct
     5161 tcttcatctc ttgctggatc atcgccgggg cgatccggcc gggatacaca ccgatccacg
     5221 acgcgatcag tcgattggca gagctgggtg caccgaaccg ctacatcgtc acaaccggga
     5281 tgatcgtgtt cgggctcggc tgcatcctgt tcgccccacg acttcgcaag cccgccgtca
     5341 tctctctgac cgtg
//
LOCUS       DATMED010000295         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2168133, whole genome shotgun sequence.
ACCESSION   DATMED010000295 DATMED010000000
VERSION     DATMED010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168133"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..965
                     /locus_tag="VNP73_07545"
     CDS             33..965
                     /locus_tag="VNP73_07545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWL65811.1"
                     /translation="MRAVAITDFGVAPKLLELPVPKPGPGEVLVRVEHASLNGFDLTV
                     ASGGAKDYMEHRFPVVLGRDFAGTVEEVGTGVTSVAPGDEVFGVMTGKYLGPGTFAEF
                     TLVPEAMGLAKIPVGLDHSAAGALGLAGTAAYTAVDAVAPSSGETVLIGGATGGVGAN
                     AIQLAVARDAEVIATAKPGSETDFVQSLGAKHAVDYTAGTADQVRALKADGVHAAIHL
                     AGDGFELADLVAAGGRFSSSLGIGADQLAERNLQATAIVAQPTKEVLDRLAAEAAAGR
                     LQVPIHHSYKLEEVPQALEDFAQGSLGKLAVAIS"
     gene            complement(973..1584)
                     /locus_tag="VNP73_07550"
     CDS             complement(973..1584)
                     /locus_tag="VNP73_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWL65812.1"
                     /translation="MRKIKATLFISLDGVIEDPHLWHFPYFNDEMGVAVNRVMGTTDT
                     LLIGRVTYDSFAGAWPEREAAGGEDADFAKKLGDARKIVVSNQDLELTWRNSEQLKGD
                     LVEAVAALKNEPGDTHIGISGSPSVVRQLLGAGLLDELHLLVHPIALGKGMHLFDNGE
                     GSIPLELISSQTFKTGVLDLVYGPAESAGDGTYEEAKAHIAQQ"
     gene            complement(1595..2170)
                     /locus_tag="VNP73_07555"
     CDS             complement(1595..2170)
                     /locus_tag="VNP73_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWL65813.1"
                     /translation="MRKPDQRRLTPDDWAGAALAAIAHGGVDAVAVEVIAGELGATKG
                     SFYWHFKNRDALIEAALALWEERSTGAVIEHLEKEPDPAWRLRTVLEGGFQRGPTERA
                     EIALLANPGHPVAVRAVRRVAERRITYLADQLEALGWRPQEARDRAVLLGYLYVGNMQ
                     LAHAAPNITRSDTRQRLVDLVFNTLIEGVKV"
     gene            2231..2707
                     /locus_tag="VNP73_07560"
     CDS             2231..2707
                     /locus_tag="VNP73_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HWL65814.1"
                     /translation="MRRLGAQATVDLPGGIHRWLPARIFMLVSALYHLPLGLSGLVYD
                     QTFPIGSGAAERAGSEMIFGVFETNGWHSLAAVLLGVVSLYFTLRPRGARPAALAIGV
                     QHVFLVIAFIVWPSETFWMASNNADQVVHSFTAIVGIGSALLTRDGQGVEPAVGRG"
     gene            2707..3111
                     /locus_tag="VNP73_07565"
     CDS             2707..3111
                     /locus_tag="VNP73_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65815.1"
                     /translation="MDAAVGVAGLICVLMAFGHHAIGVVWVLPHVTEESVPPTPFGRS
                     SMTVAMLRVTWYIVTVFVLAFGAILMTLAWAEAADARTVLLRSFAVMWLAAAAMAIWV
                     AWQRVHSLRGLFRLPVPFMWVVVALLCWWAST"
     gene            3108..3707
                     /locus_tag="VNP73_07570"
     CDS             3108..3707
                     /locus_tag="VNP73_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HWL65816.1"
                     /translation="MRVAKTAHTARPWRIHEFTRDFELEDVWALPTPGGEDDFPQLVE
                     LLAAFDPSQVSSFAVRMLAAIRLRIGDLIGLDAPETGLGSRVGSLRDRIPVDLRDGPS
                     GRDFDELLFSPLYLLHDEWAGEIANRTVHAVIHLGWVPDETTGYRGQMAILVKPNGLL
                     GNAYMEFIKPFRHWIVYPQLMREIQRAWDARSRLPYVPS"
     gene            3709..>4064
                     /locus_tag="VNP73_07575"
     CDS             3709..>4064
                     /locus_tag="VNP73_07575"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWL65817.1"
                     /translation="MRSADLSDEKVSRVVAAAAAAGVEIAPVVFEHETRTSAEAAREV
                     GCDVREIVKSLVFGVVGTDEAVLLLLSGADRVDLEEAAAALGVEGLERAAPELAKKVT
                     GYSIGATPPFGHRSRVR"
BASE COUNT          682 a         1242 c         1327 g          813 t
ORIGIN      
        1 gttcctccat cagagtcgaa aggagcggac gtatgcgcgc agttgccatc accgacttcg
       61 gagttgcacc aaaacttttg gaactgccgg ttccgaagcc cgggccgggc gaggtgctcg
      121 tacgagtcga gcacgcttcc cttaacggct tcgatctgac cgtagcctcc ggcggggcca
      181 aggactacat ggaacatcgc ttcccggtcg tgcttggcag ggacttcgcc ggtaccgtcg
      241 aggaggtcgg cactggagtt acatccgtag ctccgggcga tgaggttttc ggggtgatga
      301 ccggaaagta cctcggaccg ggcacattcg ccgagttcac cctcgttccg gaagcaatgg
      361 gactcgccaa gatcccggtc gggctcgacc actccgccgc gggggctctg ggcctggccg
      421 gaacggccgc atacacagcg gtcgacgcag tcgcaccgtc ttcgggcgaa acggttctca
      481 tcggaggtgc gaccggtgga gtcggggcca atgcgatcca actcgcagtg gcgcgcgacg
      541 ccgaggtcat cgctactgct aagccgggtt cggagaccga cttcgtccaa agccttggcg
      601 cgaagcacgc cgtggattac acggctggta ccgcggacca ggtgcgcgcg cttaaagcgg
      661 atggggtgca cgcggcgatc catcttgccg gcgacggctt cgaactggcg gacctcgtag
      721 ccgcgggagg ccgcttctcc tcgtcgctcg gcatcggagc cgatcaactg gccgaacgga
      781 acttgcaagc gacggcgatc gttgcgcaac cgacgaagga agtcctcgat cgactagcgg
      841 ccgaagcggc ggccggccgg ttgcaggtgc cgatccacca cagttacaag ctcgaagaag
      901 ttccccaagc gctcgaggat tttgcgcagg gatcgctcgg caagctagcg gttgctatct
      961 cttgaaccag ggctattgct gggcgatgtg agctttcgcc tcttcgtagg tgccatcgcc
     1021 ggccgactcg gcggggccgt agaccaggtc cagcaccccg gtcttgaagg tctgggacga
     1081 gatcagttcc agagggatcg atccttcgcc attgtcgaac aggtgcatgc ccttgccgag
     1141 agcgatcgga tgcaccagta gatgcagttc gtccagcagc ccggcgccca gcaattgacg
     1201 caccactgag ggtgagccgc tgatcccgat gtgcgtgtca cccggctcgt tcttcaaggc
     1261 ggcgacggcc tcgaccagat cgcccttcag ctgctcggaa ttccgccagg tgagctcgag
     1321 gtcctggttc gacacgacga tcttgcgcgc gtcgccgagc ttcttggcga aatcggcgtc
     1381 ttcacctccc gcggcttccc gctcgggcca ggctccggcg aagctgtcgt aggtcacgcg
     1441 accgataagc agcgtgtcgg tcgtacccat cacgcgattc accgcgactc ccatctcgtc
     1501 gttgaagtag gggaagtgcc acaggtgcgg atcctcgatg acgccgtcga gcgagatgaa
     1561 cagggttgcc ttgatcttcc tcatgatgtt cctcctaaac tttcacacct tcgatgagcg
     1621 tattgaagac aagatcaacg agtcgttgcc gtgtatcgga gcgggtgatg ttgggtgcgg
     1681 cgtgtgccag ttgcatgttg ccgacgtaca ggtaccccag gagcacagcc ctatcgcgtg
     1741 cctcctgcgg acgccatccc aacgcctcca actggtcggc cagataagtg atccttcttt
     1801 cggcaacccg tcgcacagct cggaccgcta cggggtgacc ggggttggcg agaagggcga
     1861 tctccgcacg ctcggtcggt cctcgttgga atcctccctc gagcaccgtc ctcaaccgcc
     1921 atgccgggtc gggctctttc tcgaggtgtt cgatcacggc gccggtacta cgctcctccc
     1981 acaaggcgag cgcggcttcg atcagggcgt cccggttctt gaaatgccag tagaagctcc
     2041 ctttggtggc gccgagctcg ccggcgatga cctcgaccgc aacggcatcg accccgccgt
     2101 gtgcgatggc tgcgagggcc gcgccggccc agtcgtcggg cgtgaggcga cgttggtcgg
     2161 gtttgcgcat gaccatacgg taccgtatgg tcagccatac gcaagcgtac ggagcggctg
     2221 gttagcggag gtgaggagat tgggagcaca ggcaacggtc gatctgccgg gcgggatcca
     2281 cagatggttg ccggccagga tcttcatgtt ggtctcagcg ctgtaccact tgcctttggg
     2341 gttatcgggt ctcgtctacg accagacctt tcccatcgga tcgggagccg cggaacgtgc
     2401 cggttccgaa atgatcttcg gcgtgttcga aaccaacggc tggcattccc tggccgcagt
     2461 cttgctggga gtcgtctcgc tctacttcac gctgcggccg cgaggtgcgc gccctgcggc
     2521 attggcgatc ggagttcaac acgtcttcct cgttatcgct ttcatcgtgt ggccttcgga
     2581 gacgttctgg atggcgtcga ataacgcgga ccaggtcgtt cattcgttca cggcgatagt
     2641 gggcatcggg tcggccctgc tgacgcgaga tggacaggga gtcgaaccgg cggtcggacg
     2701 cggctgatgg atgccgcagt gggcgtcgcg gggctgatct gcgtgctcat ggccttcggc
     2761 catcacgcga tcggagtggt ctgggttctc ccccacgtaa ctgaagagag cgtgccgccg
     2821 acgcccttcg gtcgttcatc gatgacggta gccatgctgc gagtcacgtg gtacatcgtc
     2881 accgtcttcg tcctggcctt cggggccatc ctcatgactc tcgcttgggc cgaagctgcc
     2941 gacgccagaa cggtgctgct gcgttcgttc gctgtgatgt ggcttgccgc cgccgcgatg
     3001 gcgatctggg tcgcgtggca aagggtgcac agtctgcgcg gcctcttccg cttaccggtt
     3061 ccgttcatgt gggttgttgt cgcgctgctg tgctggtggg catccacgtg agagttgcga
     3121 agacggcaca caccgcccga ccctggcgga tccacgagtt cactcgtgac ttcgaactcg
     3181 aggatgtgtg ggcactgcct acaccggggg gcgaggacga cttccctcaa ctggtggagt
     3241 tgctggcggc gttcgatcca tcccaggtct cttcatttgc ggtccgcatg ctggccgcga
     3301 tcaggttgag gatcggggat ctgatcggac tcgatgcccc ggagaccggc ctcggctcga
     3361 gggtggggag tctccgcgac cgaataccgg tcgaccttcg cgatggccct tccggccgag
     3421 acttcgacga gcttctcttc tccccgctgt acttgctgca cgacgagtgg gccggggaga
     3481 tcgccaaccg aaccgtgcac gccgtcatac atcttgggtg ggtccccgat gagaccaccg
     3541 gttatcgcgg gcagatggcc atcttggtga agcccaacgg actgctcggg aacgcttaca
     3601 tggagttcat caagccgttc cggcattgga tcgtgtaccc gcaactcatg cgagagatcc
     3661 aacgagcgtg ggatgcacgt tccaggcttc cttacgtacc ctcttgagat gcgctccgcc
     3721 gatctttcgg acgagaaggt atcccgcgtc gtagccgctg ctgcggcagc cggagttgag
     3781 atcgctccgg ttgttttcga gcacgagacg cgaacctcgg ccgaagccgc acgcgaggtc
     3841 gggtgtgacg tgagggagat cgtgaagtca ctcgtgttcg gcgtcgtggg aacggatgaa
     3901 gcggtgctgc ttctcctctc tggagccgat cgggtcgacc tcgaagaggc tgcggcggcc
     3961 ttaggagtag aagggctcga gcgcgccgcg cccgagctcg cgaagaaggt caccggctac
     4021 tcgatcggcg caacgcctcc gttcggtcac aggagccggg tacg
//
LOCUS       DATMED010000296         3478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2917051, whole genome shotgun sequence.
ACCESSION   DATMED010000296 DATMED010000000
VERSION     DATMED010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2917051"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VNP73_07580"
     CDS             <1..502
                     /locus_tag="VNP73_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWL65818.1"
                     /translation="FAWIVDFPWFEFNEDEERWYPIHHPFTGIVEETLDHLESDPAKV
                     ISKSYDITLNGWELASGSIRIHDREMQQRVFAALGIGPDEQQEKFGFLLNAFRYGAPP
                     HGGIAPGIDRIVALAAGETNIREVIAFPKTQTAFDPLTGAPARVDEAQLKILHLKSTA
                     PPPDQE"
     gene            complement(556..2316)
                     /locus_tag="VNP73_07585"
     CDS             complement(556..2316)
                     /locus_tag="VNP73_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65819.1"
                     /translation="MKHRFLKLTTVVGVIALITGSIVLSANAHIGASHSPFTTDGPDI
                     RSAAIEGDEISFCFDEEVSKINGGGAAFELQGYDSAAKVAGTAANLGSADPCVDVSFP
                     DSAVDQDLVEFTIASIDPDAVEDREGNESIESSVALEGGTRDEEGSVTDAPNLTGFEA
                     EDGQDKITYTFDQSVTCPADGTDFGFYTEAGNPGGREDGQPVIADCDGPEGEVEVQFD
                     ATPGVGDADRVFVENGSVCGVGVASAKCNAPEAEGGTTDDPDLVSAERTDDNEWTWHF
                     DEEVETPNNPDFFLVRVDGTLSPPTGLCTEEDDTDVACPFANLEDAPDSQIAMAVVDD
                     GAVKDDDAAEDSTIGDAPLAGATTAEPGLTDGPDLETCEIDTTENVATFGFDQPVDDS
                     GITASTLDVFDTEGNMSDGQSVEDVEENSVQIEFDEDALADAVGCHADDGAVNDSTLN
                     DSPRAAVGTGALPTVTGSTTTTTTTTTTTTTDQEPQERTVGSNISLDFARGAFKGNVS
                     SRSAGCERRRRVTVKKRRAGRDANVGSATTNRAGAYSLRAPRAKGVFYAVAARKVFTT
                     PRAAVTCTKATSRNKRVGRR"
     gene            2498..>3478
                     /locus_tag="VNP73_07590"
     CDS             2498..>3478
                     /locus_tag="VNP73_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWL65820.1"
                     /translation="MDLFGHALEERFDQYAPLAARMRPRTLEEVVGQNHVLGPDRALS
                     SLISSGDLSSIILWGPAGSGKTTLANVIARSTSAHFEPMSAVAAGVADVRKAIEAAKD
                     RLAEQGSRTVLFLDEIHRFNKAQQDALLPAVENGWVILVGATTENPSFEVNSPLMSRS
                     LLFRLEALGESDLVAILKRVLIDERGLAKAGVNAADDALDHIARGSGGDARAALNALE
                     AGASIASTRGSIIDLSIAEEALQRRALPYDKQGDWHYDTISAFIKSMRGSDPDAAVYW
                     MTRMLDAGEDPRFIARRMVILASEDVGNADPLALQVAVAAHHALEFGGLPE"
BASE COUNT          569 a         1082 c         1124 g          703 t
ORIGIN      
        1 gttcgcgtgg atcgtcgact tcccctggtt cgagttcaac gaggatgaag agcgctggta
       61 cccgatccac catcccttta ccgggatcgt cgaggagacc ctggaccatc tcgagagcga
      121 tcctgcgaaa gtcatctcga agtcctacga catcacgctg aacggttggg agctcgcctc
      181 ggggagtatc cgtatccacg accgcgagat gcaacagcgt gtcttcgccg ccctcggaat
      241 cggcccggat gagcaacagg agaagttcgg gttcctcttg aacgcatttc gttacggcgc
      301 gccacctcat ggtgggatcg cgccgggcat cgaccggatc gtcgcgctgg cagcaggaga
      361 gacgaacatc cgggaagtga tcgcatttcc caagacgcag acggcgttcg atccactgac
      421 gggagctccg gctcgtgtgg acgaggcgca gctgaagatc ctccacctga agtcgacggc
      481 gcccccgccc gaccaggagt gaaaagggcc ggcgcccgaa ggcgccggcc cggtatctct
      541 actcgtggct ggtgcctacc tgcgccccac cctcttgttg cgggaggtgg ccttcgtgca
      601 ggtcaccgct gcgcgaggcg tggtgaacac cttgcgggcg gctaccgcat agaagacgcc
      661 cttggccctc ggcgcgcgca ggctgtaagc gccggcccgg ttcgtcgtgg cggaaccgac
      721 gttggcgtct cggccggccc ttctcttctt gacggttaca cgacgtctgc gctcgcaccc
      781 tgcgctccgg gaactcacgt tgcccttgaa agcgcctcga gcgaaatcga gcgagatgtt
      841 cgagccgacc gttctttcct ggggctcctg atcggtcgta gtcgtcgtcg tggtggtcgt
      901 cgtggtcgtg gtgctgcccg tgacggtcgg cagtgcgccg gtgccgacgg ctgcgcgcgg
      961 cgagtcgttc agggtcgagt cgttcaccgc gccgtcgtcg gcgtggcaac cgaccgcatc
     1021 ggcgagtgca tcctcgtcga actcgatctg gaccgagttc tcctctacat cctcgacgga
     1081 ctgtccatcg gacatgttgc cttcggtgtc gaacacgtcg agtgtcgatg cagtgatgcc
     1141 cgaatcgtcg acgggctggt cgaagccgaa tgtggcgacg ttctccgtgg tgtcgatctc
     1201 acacgtctcc aggtcgggac catcggtaag gcccggttcg gccgtcgtag ccccggcgag
     1261 aggtgcgtcg ccgatggtgc tgtcttcggc ggcgtcgtca tctttaaccg ctccatcgtc
     1321 gacgaccgcc atcgcgatct ggctgtctgg ggcatcctcc aggttcgcga acgggcacgc
     1381 aacgtccgtg tcgtcctcct cggtgcagag cccggtgggg ggggagagcg ttccgtcgac
     1441 tcggacaagg aagaagtcgg ggttgttggg ggtctcgacc tcctcgtcga agtgccacgt
     1501 ccattcgttg tcgtccgtgc gctccgcact gaccagatcg ggatcgtcgg tggttccacc
     1561 ttcggcctct ggcgcgttgc acttggccga cgccacaccg actccgcaga cggacccgtt
     1621 ctcgacgaaa acgcggtcgg cgtcgccaac gccgggtgtc gcatcgaact ggacttcgac
     1681 ctcgccctcc ggcccatcac agtcggcgat cacaggctgg ccgtcttcgc gaccgccggg
     1741 gttgccggct tcggtgtaga agccgaagtc ggtgccgtcg gcgggacagg tcacgctctg
     1801 gtcgaaggtg taggtgatct tgtcttgacc gtcctcggcc tcgaacccgg tcaggttcgg
     1861 ggcgtcggtg actgagcctt cttcgtcgcg cgttcctcct tcgagcgcca ccgaggactc
     1921 aatgctttcg ttcccttcac gatcttcgac cgcatcgggg tcgatgctcg cgatcgtgaa
     1981 ctcgaccagg tcctggtcga cggcggagtc cgggaaggaa acgtccacac acgggtccgc
     2041 gctaccgaga ttggccgccg tgccggcaac cttcgcagcc gagtcgtatc cctggagctc
     2101 gaatgcggcg ccgcctccat tgatcttgct gacttcctcg tcgaagcaga acgagatctc
     2161 gtcgccctcg atcgctgccg agcggatgtc gggcccgtcc gttgtgaacg gtgagtggct
     2221 ggcgccgata tgggcgttcg cgctgaggac gatcgacccg gtgatgagtg cgatcacccc
     2281 cacaacagtg gtcagtttga gaaaacgatg tttcattgaa gctccttccg tgacgagcga
     2341 cccactgccc cttgctgtgg gcctggtgca ttgatgctgg tttgttgcct gctgcttgta
     2401 ttgatggatg ctgcgatgcc tttgggaacg taaataagac acctgaggtt gcccgtttgt
     2461 gggaaatggg aaagggcttt tggagaagaa tggccggatg gatcttttcg gccacgctct
     2521 cgaagagcgc ttcgatcagt atgcaccctt ggcggcacgc atgcgtccac gaaccctcga
     2581 agaagttgtc gggcagaacc atgtcctggg acccgatcgg gcgctgagct cgctcatctc
     2641 ttccggcgat ctgtcctcga tcatcctgtg gggcccggca ggctcgggca aaacgacgct
     2701 cgcgaacgtg attgcgcgct cgaccagtgc ccacttcgaa cccatgtccg cggtggccgc
     2761 gggcgtcgcc gacgtgcgca aggcgattga ggccgcgaag gaccgtttgg ccgagcaggg
     2821 ctccagaacc gtcctgttcc tcgacgagat ccaccggttc aacaaggcgc agcaggatgc
     2881 tcttcttccc gccgtcgaga acggctgggt gatcctcgtg ggggcgacga ccgagaaccc
     2941 ctccttcgaa gtgaactcgc cgttgatgtc tcgcagcctc ctctttcgtt tggaagctct
     3001 cggtgagagt gatctggttg cgatcctcaa gcgagtgctg atcgacgagc gcggcttggc
     3061 gaaggcgggg gtcaacgcag ccgacgacgc gctggatcac atagcgcgcg gctccggagg
     3121 ggacgctcgg gctgcactga acgcgttgga ggcaggtgcg tcgatcgcat ccaccagggg
     3181 ctccatcatc gatctttcga tcgccgagga agcactccag cgtcgtgcgc tcccgtacga
     3241 caagcaagga gactggcatt acgacacgat ctccgcgttc atcaagtcga tgaggggctc
     3301 cgatcccgac gcggccgttt actggatgac ccgcatgctg gatgccggcg aggatccgcg
     3361 tttcatcgcc aggaggatgg tgatccttgc ttccgaggac gtaggtaatg ccgatccact
     3421 cgccctgcag gtagccgtgg cggcgcacca tgcgcttgag ttcggtggtc taccggaa
//
LOCUS       DATMED010000297         4523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5602941, whole genome shotgun sequence.
ACCESSION   DATMED010000297 DATMED010000000
VERSION     DATMED010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5602941"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..782)
                     /locus_tag="VNP73_07595"
     CDS             complement(147..782)
                     /locus_tag="VNP73_07595"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWL65821.1"
                     /translation="MSVLARYVAGAAGISVLIAALSPPVEELAGQLFYVHMIQHLALM
                     LVVPLLLAFAAGTRARLPSWLGPAGVGALFASVMWVWHIPSLYEAALANVGLHVLEHA
                     SFLIAGWFFWVVLLGRGGHGYLARLGLTFVTTLHSGALGALIALASGVLYDSHRATAT
                     LHGLTALEDQQLAGAVMWVPTGTFFLMVMLGLLLKLLKDFDRRPQPAGDAS"
     gene            complement(779..1195)
                     /locus_tag="VNP73_07600"
     CDS             complement(779..1195)
                     /locus_tag="VNP73_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65822.1"
                     /translation="MAQVPAAEAVSRAEGRGSVALWFGVLGAPIAWVIQLLLDYSLEE
                     WWACSPATSTVGRVVGLPVGTAALLVSGLLTIVAVVAGLVSVRCYKKISKRTDEASSR
                     ALWMALAGIMNSILYLGLLIASFAPPILLGTCESSP"
     gene            complement(1195..1800)
                     /locus_tag="VNP73_07605"
     CDS             complement(1195..1800)
                     /locus_tag="VNP73_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HWL65823.1"
                     /translation="MDRAIEEVLPHHASGPRAYGWWGMVWLIATEATLFAALIATYFF
                     LRFRSGPQWPPGDIPAPELELPLIMSAILLASSIPVHFAEAGIKKGSQARLRWGLLAG
                     FLLGAIFFGVTVGIEWPEKLAEFTPRTNVYGSLFFTITGFHLSHVLVGLLISAWAQVR
                     AWNGAFDADKHLTVQNFAMYWHFVDVVWVAVFATIYLSPIL"
     gene            complement(1800..3680)
                     /gene="ctaD"
                     /locus_tag="VNP73_07610"
     CDS             complement(1800..3680)
                     /gene="ctaD"
                     /locus_tag="VNP73_07610"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWL65824.1"
                     /translation="MATIAQRLGAIWSESPGLVGFISTVDHKRIGLRYFYTGVIFFIL
                     GGLEALLIRVQLAQARLEILDPEAYNQLFSMHGITMMFLFAMPVLSGFGNYFVPLMIG
                     ARDMAFPRLNAFGYWIFLAGGIFMYSAFLTGSAPNDGWFNYLPLGDADFSPGLNIDYY
                     ALGLIFLGISTTGGAINFIVTILKMRAPGMTVNRIPIFVWGELAMAFQIVFALPLLTL
                     ACVLLFLQRRFGFHFFDAAAGGDPLLWQHLFWIFGHPEVYIVVLPAFGIASAIIPTHA
                     RRPMVGYTYIVLAELATALIGFGVWVHHMFATGLPQMTMAFISAASFMVAIPSGVQVF
                     AWLATLVTGRLVLRTPMLYILGFVATFVIGGVTGVMFSAIPFDQATTDSMFVVAHLHY
                     VLVGGAVFPMFAAIYHWGPKMTGRMLDERFGKLSFWLVFFGFNLNMFPLHISGLLGQP
                     RRTYTYEPGLGWDIHNLISTLGAFMLGIGVLVVIVDWIRSSRRGLLAGDDPWHGETLE
                     WATSSPPPHYNFLTVPTVRSKEPNWDQPDLKGGQAPKDGGHPLDGGHTTLSTSMLDAT
                     PQAVVHMPHESPWPFYLSVALFVLFFAFLLDAYLVAAVAGLASMGALMGWFWPRGETQ
                     ET"
     gene            complement(3680..>4523)
                     /gene="coxB"
                     /locus_tag="VNP73_07615"
     CDS             complement(3680..>4523)
                     /gene="coxB"
                     /locus_tag="VNP73_07615"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWL65825.1"
                     /translation="VFLVVAGMLVAAVVRAKRDPDRSLDRREIGWGEPFIAVAGVFLP
                     FVILAGVYVFSLQEMNELANRGKGAATNIEVIAHNWWWEVRYENSAVSANEIHIPTGR
                     PIRLELTSADVIHSFWVPELQVKTDHIPGRTNHMWLQADEPGRYRGQCAEYCGLQHAN
                     MIFYVIAHEPQEFERWLAHEVEPAGLPEEAEAQAGAAIFMESSCAGCHAVRGTQAAAN
                     LGPDLTHFSTRTTIAGVLPNARSNLHLFVRDPHGIKPGVAMPPTELDADELDAVLDYL
                     EALD"
BASE COUNT         1008 a         1416 c         1401 g          698 t
ORIGIN      
        1 gcagtcgcgc agatacaact ccttccccga atccgacgcc ggagtagccg ccacgggcgg
       61 cggccggtac ggctcggtct cacccaacag gaagctgcac gatcccagaa ggagcgaagc
      121 cgacgagatc accaacgcac cgaacctcat gacgcgtccc ccgccggctg gggcctccgg
      181 tcgaagtctt tgaggagctt cagcaacagg cccagcatca ccatcaggaa gaacgttccc
      241 gtcgggaccc acatcaccgc cccggctagt tgctggtcct cgagtgccgt cagcccgtgg
      301 agtgttgcag tagcccgatg cgagtcgtag agcacgccgg aggcaagcgc gatgagagcg
      361 ccgagagcgc cgctgtgcag agtggtcacg aaggtcaggc cgagccgggc gaggtacccg
      421 tgccctcccc ggcccagaag aaccacccag aagaaccatc ccgcgatcag gaaagaggcg
      481 tgctcgagaa cgtgaagccc aacgttcgcc agcgcggcct cgtaaagaga ggggatgtgc
      541 cagacccaca tcaccgacgc gaacaatgcg ccgaccccgg cggggcccaa ccatgacggc
      601 aaacgcgctc gcgtgcctgc ggcgaacgcg agaagcaacg gcacgacaag catcaaggcc
      661 aggtgctgga tcatgtgtac gtagaagagc tgtcctgcaa gttcctcgac gggcggcgag
      721 agagccgcta tcaggaccga gatcccagcg gcgccggcga cgtatcgcgc taggacgctc
      781 atggcgacga ctcacaggtg ccgagaagga tcggtggtgc gaacgaagcg atcagtaacc
      841 ccagatacag gatgctgttc atgatccccg ccaaggccat ccacagcgct cggctcgaag
      901 cctcatcggt ccttttcgag atcttcttgt agcacctcac cgacacgaga ccggcaacca
      961 ccgccacgat ggtgagcaga ccggagacca acagcgcggc ggtcccaacg ggcaacccga
     1021 cgacgcgccc tacggtcgac gttgccggcg aacaggccca ccactcctcg aggctgtaat
     1081 ccaggaggag ttggatcacc catgcgatcg gagccccgag cacgccgaac cacaatgcga
     1141 ccgaaccgcg cccctccgct ctgctgaccg cctcagccgc cggtacctgt gccatcagag
     1201 gatcggagag agatagatgg tggcgaacac agctacccaa acaacgtcca cgaagtgcca
     1261 gtacatggcg aagttctgga cggtgagatg tttgtctgcg tcgaaggctc cattccacgc
     1321 acgtacttgt gcccacgcgc tgataaggag cccaaccagg acatgcgaga gatggaaacc
     1381 ggtgatcgtg aagaacaacg atccgtagac gttcgtgcgg ggggtgaact cggcgagctt
     1441 ctcgggccac tcgatcccca cggtgacgcc gaagaagatc gcgcccagca ggaaacccgc
     1501 gagcaatccc caccgcagcc gagcctgact ccccttcttt atcccggcct ccgcgaaatg
     1561 gaccgggata ctcgacgcca agaggatcgc gctcatgatc agcggaagtt ccagctcggg
     1621 agcagggata tctcccggcg gccactgggg tcccgaacgg aagcgcagga agaagtaggt
     1681 ggcgatgagc gcagcgaaca acgtcgcctc agtcgctatc aaccacacca tcccccacca
     1741 tccgtacgcg cggggtcccg aagcgtggtg cggtagcact tcctctatag ctctgtccat
     1801 caggtctcct gggtttcgcc gcgcggccag aaccacccca tcaacgcccc catcgatgcc
     1861 agccccgcga ccgcggccac gagataggca tcgaggagga aagcgaagaa gagaacgaag
     1921 agcgctaccg atagatagaa gggccacgga gactcgtgag gcatgtgaac gacagcctgc
     1981 ggcgtggcgt cgagcatgct cgtggacagg gtggtgtgac cgccgtccag agggtggccc
     2041 ccgtccttcg gggcttgacc gcccttcagg tcgggctggt cccagttcgg ctccttgctt
     2101 cgcaccgtcg gaaccgtgag gaagttgtag tgcggtggcg gtgaactcgt cgcccactcg
     2161 agtgtctcgc cgtgccacgg gtcgtctccg gcaagcagtc ctcgtcgcga acttctgatc
     2221 cagtcgacga tcacgaccag tacgccaatc ccgagcatga aggcacccag cgtcgagatg
     2281 aggttgtgga tgtcccagcc caggcccggc tcgtacgtgt aggtcctgcg cggctggccc
     2341 agcaggccgg agatgtgcag agggaacatg ttgaggttga agccgaagaa gaccagccag
     2401 aaggacagtt tcccgaaacg ttcgtccagc atccgcccgg tcatcttcgg cccccagtgg
     2461 tagatcgctg cgaacatcgg gaagacggcc ccacccacca ggacgtaatg caggtgagcg
     2521 acgacgaaca tcgagtccgt cgtcgcctga tcgaacggaa tcgccgagaa catcacaccc
     2581 gttacaccgc cgatgacgaa cgtcgcgacg aagcccagga tgtagagcat cggagtcctt
     2641 agcaccaacc ggccggtcac gagtgtggcg agccacgcga acacctgaac acccgacggg
     2701 atcgcgacca tgaaggaagc agcggagatg aatgccatcg tcatctgagg caatccggtc
     2761 gcgaacatgt gatgcaccca tacgccgaaa ccgatcaaag cggtggcgag ctcggccagg
     2821 acgatgtacg tgtagccaac catgggacgg cgcgcgtgcg tagggatgat cgccgacgcg
     2881 ataccgaagg ctggaagcac gacgatgtac acctcgggat ggccgaagat ccagaacaga
     2941 tgctgccaca acagcggatc accaccggcg gccgcatcga agaagtggaa tccgaacctc
     3001 cgctggagga acaggaggac gcacgcaagt gtcaatagcg gcagcgcgaa cacgatctgg
     3061 aacgccatcg caagctcgcc ccacacgaag atggggatgc gattgacggt catccccggc
     3121 gcccgcatct tgaggatggt cacgatgaag ttgatcgcgc ccccggtggt cgagatgccg
     3181 aggaagataa gaccgagggc gtagtagtcg atgttcagcc ctggagagaa gtcggcatct
     3241 cccaacggca ggtagttgaa ccacccatcg ttaggtgcgc tgccggtgag gaatgcgctg
     3301 tacatgaaga tcccacccgc cagaaagatc caatagccga aggcgttcag ccgcggaaaa
     3361 gccatgtcgc gcgcaccgat catcagcgga acgaagtagt taccgaatcc ggagagcacc
     3421 ggcatggcaa agaggaacat catcgtgatc ccgtgcatcg agaagagctg gttgtaggcc
     3481 tccgggtcaa ggatctcgag gcgagcttgg gccaattgga cgcggatgag cagggcttcc
     3541 aaccctccga ggatgaagaa gatcaccccc gtatagaagt agcggagacc gatacgtttg
     3601 tgatcgacgg tcgatatgaa gcccacgagt ccggggctct cggaccagat cgcacccagg
     3661 cgttgagcga tcgtcgccat cagtcgagag cctccaggta gtcgagtacc gcgtccagct
     3721 cgtcggcatc caactcggtg ggaggcatcg cgacccccgg cttgatcccg tgagggtcgc
     3781 gcacgaagag atggaggttg gagcgggcgt taggaagaac cccggcgatc gtcgtgcgtg
     3841 tggagaaatg cgtcagatcg ggtccgaggt tcgccgctgc ctgcgttcca cgaacggcat
     3901 gacaaccagc acatgacgac tccatgaata tggccgcacc ggcctgggcc tccgcctcct
     3961 cggggagtcc ggcgggttcc acttcgtgcg caagccatcg ctcgaactcc tgtggttcgt
     4021 gagcgatgac gtagaagatc atgttggcgt gctgcaggcc gcagtactca gcacactgcc
     4081 ccctgtaacg cccgggttcg tcggcctgca gccacatgtg gtttgtccgt ccggggatgt
     4141 gatcggtctt cacctgaagt tctgggaccc agaaactgtg gatcacatct gccgacgtca
     4201 actccaatct gatcggtcga ccggttggga tgtggatctc gtttgccgac actgcggagt
     4261 tttcgtagcg gacctcccac caccagttgt gcgcgatcac ctcgatgttg gtcgcggccc
     4321 ccttgcctct gttggccagc tcgttcatct cctgcagcga gaacacgtac accccggcca
     4381 ggatcacgaa gggaagaaag acgccggcga ctgcgatgaa aggctcgccc cagccgatct
     4441 ccctgcgatc gagcgagcgg tcggggtccc gcttggctct gacgaccgcg gcaacgagca
     4501 tcccggcaac cactagaaag acc
//
LOCUS       DATMED010000298         4216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1768728, whole genome shotgun sequence.
ACCESSION   DATMED010000298 DATMED010000000
VERSION     DATMED010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768728"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1012
                     /locus_tag="VNP73_07620"
     CDS             116..1012
                     /locus_tag="VNP73_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HWL65826.1"
                     /translation="MTSPFRCADAAEARDEPLYGTASQVRRWLLLEHPGSWGSDAITQ
                     SKLPQGVELNRRASEAGIRIVLIRRGIRFSAGARRCYFLRTEEGSLYQSTMTLDRVDD
                     LLDIDLTPLRDGRPIVEAKHHPTPIFLVCTHGKHDACCSIRGNLVSRVACAEPQWDAW
                     ECSHIGGDRFAANVVCFPHGLYYGRVRPHKVVRLMNDYAQATLSLEHFRGRCTHPFPV
                     QAAEYFARRAIDNTDIDALSVLRFNRTADEVAVTIAMRNGRRVEVLMRISRMGDYQLT
                     CRSERTNPIPRYDLVSCKIEDS"
     gene            1262..3652
                     /locus_tag="VNP73_07625"
     CDS             1262..3652
                     /locus_tag="VNP73_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWL65827.1"
                     /translation="MSQAEVSIDAPTGPLSDAELALIDAYWRAANYLSVGQIYLLDNP
                     LLREPLRIEHVKPRLLGHWGTTPGLNFVYVHMNRVIRESDLNAMYVTGPGHGGPGLVA
                     TAYLEGIYTETYSHVTEDEAGMRELFRQFSFPGGVPSHVAPETPGSIHEGGELGYSMA
                     HAFGAAFDNPDLLVCCIVGDGEAETGPLATSWHSNKFLNPVTDGAVLPVLHLNGYKIA
                     NPTVLARIPEDELRRLMEGYGYRPRFVSGNDPAEMHQQMASVLDECIEEIEAIQRAAR
                     AGSETERPRWPMIVLRTPKGWTGPKTVDGNPVEGTHRSHQVPLAKLAENPEHLRALEE
                     WMRSYRPDELFDEAGRLVDELRALSPRGERRMSANPAANGGLLLRDLLLPDFRDYGVK
                     VDAPGTASSESTRVLGEFVRDVMKANQHNGNFRLFGPDETESNRLGAVFEVTNRTWVA
                     ETRPNDEQVAADGRVMEVLSEHMCQGWLEGYLLTGRHGLFSCYEAFIHIIDSMFNQHA
                     KWLKVSHEIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKKAEIIRVYLP
                     PDTNCLLSVADHCLRSRDYVNVIVAGKQPTLNWLSIEDATLHSTRGIGVWDWASNDDG
                     EEPDVVMACAGDTPTLETLAAVALVREHLPQLKVRVINVVDLMRLQDDSEHPHGMTSA
                     QFDALFTTNKPVIFAYHGYPWLIHRLTYRRSNHANIHVRGYKEEGTTTTPFDMVMLND
                     LDRFHLVIDVIDRVPGLGARYAGLRQQMVDERLRHRAHTRRTGEDSPEVSNWTWPY"
     gene            3666..>4216
                     /locus_tag="VNP73_07630"
     CDS             3666..>4216
                     /locus_tag="VNP73_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWL65828.1"
                     /translation="MRILVLNAGSSSIKLRLLDADDAVSLSLDLDTDGLKEHRALEGA
                     VKESEVDAVGHRVVHGGNLFSGAAIITDEVQEKVESLEELAPLHQAASLEGITRARHA
                     FPRLPHVACFDTAFHSDMPAAASTYAVPLQWRERFGVRRYGFHGLSHAYASRRCAEMI
                     GQSLDSLRIVTCHLGAGASLAAVA"
BASE COUNT          794 a         1305 c         1310 g          807 t
ORIGIN      
        1 atagccgggc gagagcgctt tcaggcgcgg gacctgcacc agttcctgac tcccgagagc
       61 gccctcgttc tcgtgaagcg gctgatccga gaagggtttc tccagaccga cgtggatgac
      121 tagcccgttt cgttgcgcgg atgcggcgga agcgcgcgac gaaccgctct acggcaccgc
      181 gtcgcaagtc cgtcgctggc tgctattaga gcacccgggt tcgtggggga gcgatgcgat
      241 cacccagagc aagctgccac agggcgtgga gctcaaccgc cgcgctagcg aggcgggcat
      301 acgaatcgtt ttgattcgac ggggcattcg cttctctgcc ggtgcgcggc ggtgctactt
      361 cttgcggacc gaggagggca gcctctacca atccaccatg acgcttgacc gagtcgacga
      421 tcttctggac atcgacctca cgccgctcag agatggtagg cccatcgtcg aagccaagca
      481 ccatcccact cccatctttt tggtttgcac gcatgggaag cacgacgcgt gctgctcgat
      541 ccggggcaat ctagtgtcga gagtcgcttg cgccgagcca cagtgggacg cctgggaatg
      601 ctcccacatc ggcggggacc gcttcgcggc gaacgtcgtg tgctttccac acgggctgta
      661 ctacggacgc gtacgacccc acaaggttgt gcgacttatg aacgactacg cgcaggcaac
      721 gctctcgctc gagcacttcc gaggccgctg tacccatccg tttccggtgc aggcggccga
      781 atacttcgct cgtcgtgcga tcgacaatac tgacatcgac gcgctctcag tgttgcgctt
      841 taaccgaacg gcagatgagg tggcggtgac tatcgcgatg aggaacgggc gccgtgtcga
      901 agtgctgatg cgaatttcgc gaatgggtga ctaccagctg acgtgtcgct ccgagcgcac
      961 aaaccccatc ccacgctacg atctcgtttc gtgcaagatc gaagactcgt agatctcgga
     1021 cgtggacgtt tcgacgaggg ttggacggga tccttgctag tccgcacccg gtgagagtcg
     1081 tagccgtagg agacggtctt gggatttacc agaggtgagc tcgagcaccg ctaggggttg
     1141 gagcggccca accggctgcg caactcctgc agtgagtggt cttcaagacg acagtcggac
     1201 ccgaaagggt tcgtctgttc gttgtgtgac aatcgcggct tgccaagtga ggaggagttc
     1261 gatgtcacag gccgaagtct cgattgacgc tcccaccggc ccgctctcgg atgccgaact
     1321 ggccttgatc gacgcttact ggcgcgcggc caactatctt tctgttggtc agatctatct
     1381 cctcgataac cccctgcttc gggaacccct ccggatcgag cacgtgaagc cccgcctcct
     1441 cggtcactgg ggcacgacgc ccggcctcaa cttcgtgtac gtccacatga accgtgtgat
     1501 cagggagtcg gacctgaacg cgatgtacgt gaccggaccg gggcatggcg ggcccgggtt
     1561 ggttgcgacc gcctatctcg agggcattta caccgagact tactcccacg tcaccgagga
     1621 cgaagccggt atgcgcgagc tgtttcgtca gttctcgttc ccgggcgggg ttccaagtca
     1681 tgtcgccccc gagactcccg gctccatcca cgagggtggc gagctgggtt actcgatggc
     1741 tcatgcattc ggtgctgcct tcgacaaccc cgacctcctc gtgtgctgca tcgtcggcga
     1801 cggcgaggcc gaaaccggtc ccttggctac gagctggcac tcgaacaagt ttctgaaccc
     1861 ggtgacggac ggcgcggtcc tgcccgtcct ccacctcaac ggctacaaga tcgccaaccc
     1921 gacggtgctc gcccggatcc ccgaggatga gctccggcgg ttgatggagg gatacggata
     1981 ccgaccgcgt ttcgtgagcg ggaacgaccc ggccgagatg caccagcaga tggcatcggt
     2041 gctcgatgaa tgcatcgagg agattgaggc aattcagcgg gcggcccggg ctggatccga
     2101 gaccgagcgc ccccgctggc ccatgatcgt gctccgcacc cccaaggggt ggaccggccc
     2161 gaagaccgtc gacggcaacc cggtcgaggg aacccaccgt tcccaccagg ttccgctggc
     2221 gaagctggcg gagaatcccg agcacctccg cgccctcgaa gagtggatgc gcagctatcg
     2281 cccggacgag ctgttcgatg aagcgggacg gctggttgac gagctacgcg cactatcgcc
     2341 gcgtggggaa cgccgtatga gcgccaatcc cgcggccaac ggtggcttgc tgctgcgcga
     2401 tctgttgttg ccggacttcc gtgactatgg agtaaaggtc gacgctcccg gaacggcatc
     2461 cagcgagtcc acgcgtgttc tcggtgagtt cgtgagagac gtgatgaagg ccaatcagca
     2521 caacggcaac tttcgactct tcggtccgga cgaaaccgaa tcgaaccggc tgggcgcggt
     2581 cttcgaggtc accaatcgga cctgggtcgc cgaaacgaga cccaacgacg agcaggtcgc
     2641 ggcagacggc cgtgtgatgg aggttctgag cgagcacatg tgccagggat ggctcgaggg
     2701 ctacctgttg accgggcggc acgggctctt cagttgctac gaggcgttca tccacatcat
     2761 tgattcgatg ttcaaccagc atgccaagtg gttgaaggtg agccacgaga ttccctggcg
     2821 cagacccatt gcttccctca actacctgtt gacctcgcac gtctggcgcc aggaccacaa
     2881 tgggttctct caccaggatc ccggcttcat cgatcacgtg gtgaacaaga aagcggagat
     2941 catcagggtt tacctgcctc ccgacacgaa ctgccttcta tccgtcgccg atcactgcct
     3001 gcggagtcgc gactacgtca atgtgatcgt tgcaggcaag cagcccacat tgaactggct
     3061 gtcgattgag gatgcaaccc tgcactccac tcgcgggatc ggtgtctggg attgggcgag
     3121 caatgacgat ggcgaagaac cggatgtcgt catggcttgc gcgggcgaca ccccgacact
     3181 cgagaccctg gctgcggtcg ctctcgtacg ggagcacctt ccgcaactga aggtccgggt
     3241 gatcaatgtg gtcgatctca tgcgccttca ggacgacagt gagcatcctc acggcatgac
     3301 aagcgcccag ttcgacgcac tcttcaccac gaacaagccg gtgatcttcg cctaccacgg
     3361 ctatccctgg ctgattcatc gcctcacata ccggcgctcg aaccacgcaa acatccatgt
     3421 gcgcggatac aaggaggagg ggacgacaac cacgcccttc gacatggtga tgctgaacga
     3481 tctggatcgc ttccatctcg tgatcgacgt gatcgatcgg gtccccgggc tcggggcccg
     3541 ctatgccggc ttgcgtcagc agatggtcga tgaacgcctg cgccatcgcg ctcacacgcg
     3601 caggacaggc gaagacagcc cggaggtcag caactggacc tggccctact aaaggggtcg
     3661 ccggcatgcg aatcctcgtg ctcaacgcag ggtcgagcag cataaagctg cgactgctgg
     3721 atgcggatga tgcggtctca ctctcgctcg atctcgatac cgatggtctg aaggaacatc
     3781 gtgctctgga gggggcggtg aaggagtcgg aggtcgacgc ggtggggcat cgcgtcgtgc
     3841 acggcggcaa tctgttctcg ggcgcagcga tcatcaccga cgaggtccag gagaaggtcg
     3901 aatcgcttga ggagttggct ccgcttcatc aagctgcttc tctcgaggga atcacgcgcg
     3961 cacggcatgc gttccccaga ctgccgcacg tggcctgctt cgacacggcc tttcactcgg
     4021 acatgcctgc ggccgcctcg acctacgctg tgcctctgca atggcgggaa cgattcggag
     4081 tgcggcgcta cgggtttcat ggtctctcgc atgcctacgc atcacggcgt tgcgccgaga
     4141 tgatcggtca atctctcgac tcacttcgca ttgtcacctg ccacttgggt gcgggtgcct
     4201 cgcttgcggc cgtcgc
//
LOCUS       DATMED010000299         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5237416, whole genome shotgun sequence.
ACCESSION   DATMED010000299 DATMED010000000
VERSION     DATMED010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5237416"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VNP73_07635"
     CDS             <1..817
                     /locus_tag="VNP73_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWL65829.1"
                     /translation="SQEVNWTIIPYPTEGWAKAVFGEPDVELLWKDIESFMRLDQPDP
                     VEAWRQHIERLETRAHAMTECGFDALHFEGPGTDLTIGLIDKSRWVAAAFTTNWGRKH
                     IPNMPTEEVFTTPDYRRTEGSVRSTRPLALSGTVIRDLAMRFEGGRVVEVTASTGGEI
                     IEAEQGTDDAARYLGEVALVDSASPIGKTGRTYLNTLFDENATAHIAYGSGYPHAVEG
                     AMELDPDARYELGINRSAVHTDFMIGGPEVTVTGIERGGGRVPVIVDDVWQLS"
     gene            822..1355
                     /locus_tag="VNP73_07640"
     CDS             822..1355
                     /locus_tag="VNP73_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65830.1"
                     /translation="MPRRPISWLIPLVAVALIGTAAAIGLGQDDEPAAVQDLERVQSA
                     GERAGDAGQRIIENLERIEANLKEGAGLSDKTGEIHELTTKQRESLERLATLLRGQLQ
                     TLRSTKESLEGARRSASDLKRLGREQLVILRRTVAAVRSLRSDAEFATRTSGELSQLA
                     LYGARLAEDSQRRFGGP"
     gene            1352..1846
                     /locus_tag="VNP73_07645"
     CDS             1352..1846
                     /locus_tag="VNP73_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65831.1"
                     /translation="MKAARVAGLGGAIVLTVASLVIMFVSIGAKNNAHSTLEQAQHLY
                     QARVDVGEAQEALGDSDLGSAIESARKTNETADRVHAITTQIASLLESAEVSATATSR
                     LSGQSVRNVSLSRKQTKATTDLLGLISGYQRAAGGFAVDTNSALERILRALKRTNRSF
                     PGRP"
     gene            1846..2376
                     /locus_tag="VNP73_07650"
     CDS             1846..2376
                     /locus_tag="VNP73_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65832.1"
                     /translation="MADRFQVGSAIGRLLARGIPGVTVLLCAAAAASSLAAWQFQRSA
                     AEDLIEARIVQGKEVQADDRPPPSASDNRAIIASLNQGIAVRQKIDEQLQRIESSVTM
                     LAVRQREAEEITGLGFTEIRAIARSLGGASGAAQRSVDRLDDLRARLQESAELARAIA
                     EELEELDRKMGPGGER"
     gene            2373..2912
                     /locus_tag="VNP73_07655"
     CDS             2373..2912
                     /locus_tag="VNP73_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65833.1"
                     /translation="MTGPLVRIVSGLVTAGLLFGAVAIGGGTPLSPARIADAITRAAD
                     NSTAAAANTKRAARDTRYLAIVARNVQSQVETSRRMLATQSKMLASSKQGSARSRAIQ
                     AGVASVRRAITQMAKRVEDLVGFSGRAVSTAERSASSSDRLAATLRELRTLFNEVMKE
                     SRELNRKARGYDELRDGPG"
     gene            2909..3493
                     /locus_tag="VNP73_07660"
     CDS             2909..3493
                     /locus_tag="VNP73_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65834.1"
                     /translation="MRWIALPLVAAAVLGWSGFLHDLDRMTQASVDLAAASEEAPRTT
                     GEAAEEVSSLPQVARLTTQQADAFEGLGNALELSADRVIKLNESLDEQAGGIDAVVGG
                     IRRVKALLGCVRERLVELTSASDRVPPALNDITGTLGLLEDHQRKSLRHLKSINRKLT
                     ALGVAARATDVKAPRPPDIPAIELPREGAAPIDC"
BASE COUNT          682 a         1166 c         1144 g          557 t
ORIGIN      
        1 ctcgcaagag gtgaactgga cgatcatccc ctacccgacc gaagggtggg cgaaggcggt
       61 cttcggcgaa cccgacgtcg agcttctgtg gaaggacatc gagtcgttca tgcgcctcga
      121 tcaacccgac ccggtcgaag catggcgaca gcacatcgag agactcgaaa cgcgcgccca
      181 cgctatgacc gagtgcggat tcgacgcgct ccatttcgaa ggcccgggca ccgatctcac
      241 gatcggcctc atcgacaagt cgcgctgggt ggccgctgcg ttcacgacca actggggtcg
      301 aaagcacatc cccaacatgc cgacggagga agtcttcaca actcccgatt accgccgcac
      361 ggaaggttcg gtgcgctcca cgcgtccact ggcactcagc gggacggtca tccgagacct
      421 ggcgatgcgt ttcgaggggg gccgcgtggt cgaggtcacg gcgtcgaccg gaggggaaat
      481 catcgaagcg gaacaaggaa ccgacgacgc cgcccgttac ctgggcgagg tcgccctcgt
      541 cgacagcgcg tcgccgatcg gaaagaccgg gcggacgtac ctcaacacgt tgttcgacga
      601 gaacgccact gcacacatcg cttacggctc cgggtatccg cacgcggtcg agggcgcgat
      661 ggagctcgac ccggatgctc gctacgagct gggtatcaat cggtctgccg tacacaccga
      721 cttcatgatc ggcggacccg aagtaacggt gacgggcatc gagcgcggcg gaggccgagt
      781 tcctgtgatc gtcgacgacg tctggcagct gtcgtagccg ggtgccgcgc cgcccgatct
      841 cctggctgat cccgctcgtt gccgtcgcac tcatcggtac ggcagccgcg atcggattgg
      901 gacaagacga cgaacctgcc gccgtccaag atctcgaacg cgttcagagc gccggcgaac
      961 gtgcaggcga cgccgggcaa cggatcatcg agaacctcga gcggatcgaa gcgaacctta
     1021 aagagggggc aggactctcc gacaaaactg gcgagatcca cgagctgacg accaagcaac
     1081 gcgaatccct cgagcggctc gccaccctgc tgcgagggca gctgcagacc ttgcgtagca
     1141 ccaaggagtc cctcgaaggg gcgcgccgga gcgcatccga tctgaagcga ttggggcgcg
     1201 agcaactcgt catcctgagg cgaaccgtcg ctgcggtgcg gtccctccga tccgatgccg
     1261 agttcgccac gcgcacgtcc ggtgagttat cccagctcgc cctctacggc gcacgtttgg
     1321 ccgaggattc gcaacggagg ttcggggggc cgtgaaggcg gcgcgcgtgg ccggtctcgg
     1381 tggagcgatc gtgctcactg ttgcgtcgct tgtcatcatg ttcgtgtcga tcggcgccaa
     1441 gaacaacgca cactcgaccc tcgaacaagc gcaacatctc tatcaagcgc gggtcgatgt
     1501 aggtgaagca caggaggccc tcggcgactc ggacctcgga tcggcgatcg agtcggctcg
     1561 caagacgaac gagaccgcgg accgtgtaca cgcgatcaca actcagatcg cgagcctgct
     1621 ggaatcggcc gaagtgtcgg cgacggcaac gagtcggttg tcgggccagt cggttcggaa
     1681 cgtgtcactc agccggaagc agacgaaagc gaccaccgat ctgctcgggc tcatctccgg
     1741 ctaccagcga gccgcgggcg gcttcgccgt cgacacgaac tctgcactcg aacgcatctt
     1801 gcgcgcactc aaacgaacga accgttcctt cccgggccga ccgtgatggc agatcggttt
     1861 caggtcggct cggcgatcgg acgactcctg gcccgcggca ttcccggagt gaccgttctc
     1921 ctttgcgccg cggccgccgc gtcttcgctt gccgcgtggc agttccagcg ctcggccgcc
     1981 gaggatctga tcgaagcgcg catcgttcag ggcaaagagg tccaagccga cgaccggccc
     2041 cctccctcgg cttcggacaa ccgagccatc atcgccagtc tcaaccaagg gatcgccgtc
     2101 cgccagaaga tcgacgagca actgcagcgg atcgagtcga gcgtgacgat gcttgcagtg
     2161 cgccaacgag aggccgaaga gatcaccggg ctcgggttca ccgagatccg ggcgatcgcc
     2221 cgttcattgg gtggcgcatc gggggccgca cagcgctcgg tcgatcgact cgacgatcta
     2281 cgcgctcgtt tgcaagagtc ggcggagctg gcccgggcga tagcggagga actggaagag
     2341 ctcgatcgca agatggggcc gggaggcgaa cggtgaccgg ccccctagtc cggatcgtga
     2401 gtggactcgt gacggccggt ctgctctttg gcgcagtcgc catcggtggc ggcaccccgc
     2461 tatcccccgc tcgtatcgcc gacgccatca ctcgggccgc cgacaactcg accgcggcgg
     2521 cggccaatac caagagagca gccagagaca cccgctacct agcgatcgtc gctcgcaacg
     2581 tccagtccca ggtcgaaacg agcagacgca tgcttgccac ccaatccaag atgctggcct
     2641 cgtccaagca aggatcggcg cggtcccgcg ccatccaagc cggggtcgcg tccgtccgcc
     2701 gagcgatcac gcagatggcg aaaagggtcg aagacctggt cggtttctcc ggtcgcgccg
     2761 tatcgaccgc ggagcgttcg gcctcttcct cggaccgtct ggctgcaacc ctgcgcgagc
     2821 tgcgaactct cttcaacgag gtcatgaagg aatcgcgtga acttaaccgc aaggcacgcg
     2881 gctatgacga actcagggac ggtccgggat gaggtggatc gcgctccccc tcgtagccgc
     2941 ggcggtcctc ggatggtccg ggttccttca cgacctcgat cgcatgacac aggcttcagt
     3001 cgatcttgcc gcagccagcg aggaagctcc gcgaacgacc ggggaagcag ccgaggaagt
     3061 gtcgtcgtta ccccaggttg cgaggctgac gacacaacaa gccgacgcgt tcgaagggct
     3121 ggggaacgca ctcgagttgt cggccgaccg cgtcatcaag ctgaacgaat cgctcgacga
     3181 gcaggccggc gggatcgacg cggtggtcgg tgggatccga cgagtcaagg cgctgctcgg
     3241 ctgcgtgagg gagcggctcg tcgaacttac gtctgcatca gatcgtgttc cgcccgccct
     3301 gaacgacatc acaggcactc tcggcctgct cgaggaccat cagcgcaagt cgctccggca
     3361 cctgaagagc atcaaccgaa aactgacggc cctgggcgtc gcggcccgcg ctaccgatgt
     3421 gaaggctccg cggcccccgg acatccctgc gatcgagcta ccgcgcgagg gggccgcccc
     3481 gatcgactgc tagaccccta gaaccagtcc ggcccccact cgaagcggtc gttcgtcgtc
     3541 agcttgttg
//
LOCUS       DATMED010000300         4905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_242648, whole genome shotgun sequence.
ACCESSION   DATMED010000300 DATMED010000000
VERSION     DATMED010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242648"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="VNP73_07665"
     CDS             complement(<1..2054)
                     /locus_tag="VNP73_07665"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWL65835.1"
                     /translation="MSGPILAAGEATSLDIPPLSGFENTMLWVILGISLVALVYAYAL
                     VKGVLAKDEGTEKMQSISLAIREGANAYLTRQFKTLAVFVAVLSVVLLFLEVGEPANF
                     SAGAIRIYRSLAFILGAVFSATVGFVGMGLAVRANVRVASAARRGLRESMAIAFRAGG
                     VAGMFTVGLGLFGATAIFLVFKGDAPLVLFGFGFGGALLAMFMRVGGGIFTKAADVGA
                     DLVGKVEQNIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYEVTLVAALILGFVAY
                     QGTGSVVAAVLFPLFVRAIGVITSIIGIEAVKPRTETEHGMKAINRGFILSGVASAIA
                     VFFVSKFYVKDVKTFWAVVIGLVLAGAIHFLTEYYTSTERKPVKEIAGSTRTGAATTI
                     IQGTAVGMESTVFSVLTIAAAIFTAFLLGGGEADLALYYIALTGMGMLTTVGVIVSMD
                     TYGPISDNAHGVAEMSGGLGEEADKIMTGLDAVGNTTKAITKGLAIATAVLAATSLFG
                     SFRDIVGNVAIDVAQPDVLIGLLIGGAVPFLFASLTIKAVGRAAGTVVLEVRNQFRNN
                     PGIMDYSVKPDYARVVDICTRTSLRELATPGILAIVTPIVVGFAFGAIPLGAFLAGTI
                     LVGQLLAVYLSNAGGAWDNAKKLIEDGHLGGKGSPAHEASIIGDTVGDPFKDTAGPAL
                     NPL"
     gene            complement(2051..2764)
                     /locus_tag="VNP73_07670"
     CDS             complement(2051..2764)
                     /locus_tag="VNP73_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWL65836.1"
                     /translation="MDGTLSKSDREFLKAIYRLTRGARGGPVPAAHTGALAESLGVTP
                     GTATAAVKRLAERGLLTHKPYRGVELTRSGRRLAVSAIRRHRIVERFLADMLGYAWNE
                     ADRLAGTFEHELPQEVEDRLFVALDRPQSCPHGFPIPDPDVIDIPDMPPLYALEPGDV
                     AVVAVPGSTDQEIVDFLDALGLRPGVRVEIREKHPFDGPLVLRVDGHERTISEKVARQ
                     IYVRKEEPTSQKRKGKEPA"
     gene            complement(2821..3228)
                     /locus_tag="VNP73_07675"
     CDS             complement(2821..3228)
                     /locus_tag="VNP73_07675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65837.1"
                     /translation="MAAVSVEIPPRSPYVGVVRLALAALGRASGLDEEQVDELKIAVG
                     EACVNSVLAHEEAGSEEAVLVEYDDDGSTITVQVSDRAGSVEQLGGSDMQDSQGFSSR
                     LAFSVALLESLVDECRFEPRDGGGMITHLAFRR"
     gene            complement(3232..3573)
                     /locus_tag="VNP73_07680"
     CDS             complement(3232..3573)
                     /locus_tag="VNP73_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HWL65838.1"
                     /translation="MELGLDVSTVGDHAVVEVKGEIDVYTAPKLREKLIELVSDGSYN
                     VVVNLEGVDFLDSTGLGVLVGALKRVKAHDGSLALVCTQDKILKIFKITGLTKVFPIH
                     PSVEEAAGASA"
     gene            3760..4356
                     /locus_tag="VNP73_07685"
     CDS             3760..4356
                     /locus_tag="VNP73_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65839.1"
                     /translation="MSFVVPSQRRLVWPLAVALAAAAVTGLALYLPTRSHHVPTSPGA
                     PAVVAATVADWELAIYPAGSAGLGGVSKKAKARVEAQRKPLATLIQDLYDALFLDPTK
                     LEKAIGIHLEKTAGAALIKDAGLPKGLDELEITTRKARVGISVAGADNAAAKVKITGV
                     ATRDGRRTGFKHESTLWLERSHKEWKVIAFDLKQGPKR"
     gene            4353..>4905
                     /locus_tag="VNP73_07690"
     CDS             4353..>4905
                     /locus_tag="VNP73_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWL65840.1"
                     /translation="MIRRATVLVTLLASLIATLSLVGPAGTAPLLVGTTHRAYQPQRG
                     KIFILVIGNDARFGNPPTARADATHLVGINTKTMKAGILNFPRDSWVNIPGRGMSKIN
                     EATQAGGPQGLVATMEGLTGIKIDYWVMTGFEGFMGIIDGLGRVRVRTHQDLYDPSGS
                     GIHMPAGKHMLGPKSSLAYLRTRH"
BASE COUNT          983 a         1576 c         1490 g          856 t
ORIGIN      
        1 agcgggttca gcgcggggcc ggcggtgtcc ttgaacgggt caccgacggt gtcgccaatg
       61 atgctcgcct cgtgtgcagg cgagcccttg ccgccgagat gtccgtcctc gatgagcttc
      121 ttggcgttgt cccacgcacc accggcgtta gacaggtaga cggccagaag ctgtccgacc
      181 aggatcgtgc cggccaggaa cgctcccaag gggatggctc caaaggcgaa accaaccacg
      241 atcggtgtca cgatggccag gatccccgga gttgcgagct cacgcagcga cgtcctcgta
      301 cagatgtcga cgactcgtgc gtagtcaggc ttgaccgagt aatccatgat cccggggttg
      361 tttcggaact ggttccgcac ctcgagtacg acggtcccgg ccgcacggcc caccgccttg
      421 atcgtgagag atgcaaacag gaacggcacc gcgccaccga tcagaagacc gatcagcacg
      481 tcgggctgag caacgtcgat cgcaacgttg cccacgatgt ctcggaagga cccgaacaga
      541 gacgtcgccg caagcacagc cgttgcgatg gccagaccct tcgtgatggc cttggtcgta
      601 ttaccgaccg cgtcgaggcc ggtcatgatc ttgtcggctt cttcgcccag accacccgac
      661 atctcggcaa caccgtgcgc gttgtcggag atcggaccgt acgtgtccat cgacacgatg
      721 acacctaccg tcgtgagcat gcccatacca gtgagagcga tgtagtagag ggccaggtcg
      781 gcttctccac cgccaagaag gaacgcagtg aagatcgctg ctgcgatcgt cagcaccgag
      841 aagaccgttg actccatacc cacggccgtt ccctggatga tcgtcgtggc cgcacccgta
      901 cgggtcgacc ctgctatctc cttgaccggc ttgcgttcgg tagacgtgta gtactcggtc
      961 aggaagtgga tcgcacccgc gagcacgaga ccgatgacga cggcccagaa agtcttgacg
     1021 tccttcacat agaacttcga gacgaagaac actgcgatcg ccgacgcgac ccccgacagg
     1081 atgaagcctc ggttgatcgc cttcatcccg tgctcggtct cggtccgtgg cttgaccgcc
     1141 tcgataccga tgatcgaggt gatgaccccg atcgcacgca cgaagagcgg gaacaacacc
     1201 gcagcaacga cggatccggt gccttggtat gcgacgaacc cgaggatcag agccgcgaca
     1261 agcgtgactt cgtacgactc gaagagatcg gccgccatgc cggcgcagtc accgacgttg
     1321 tcaccgacgt tgtcggcgat ggtcgcagcg ttacgtgggt cgtcctcggg gatgttctgc
     1381 tcgaccttac cgacaaggtc ggcgccgacg tcggcagcct tcgtgaagat tcccccgccg
     1441 acccgcatga acatggcgag tagtgcaccg ccgaaaccga atccgaacag cacgagcggg
     1501 gcgtcaccct tgaagaccag gaagatggcg gtcgcaccga acagtccaag accgaccgtg
     1561 aacatccctg caacaccacc ggcgcggaaa gcgatggcca tcgattcgcg caatcctcga
     1621 cgagccgcgc ttgcgacgcg cacgttcgcg cgcaccgcga gacccatgcc gacgaacccg
     1681 accgtggccg agaagactgc gcccaggatg aacgcaagtg atcggtagat cctgatcgct
     1741 ccggccgaga agttggccgg ctcgccaacc tccaggaaca gcaacacgac cgaaaggacg
     1801 gcaacgaata cagccagcgt cttgaactga cgcgtcaggt atgcgttcgc tccctcacgg
     1861 atcgcgagtg agatcgactg catcttctcg gtgccctcgt ctttagcgag aacgcccttc
     1921 acgagtgcgt atgcgtacac cagggcgacc aatgagatcc ccaagatgac ccacaacatc
     1981 gtgttctcga aaccggacaa cggggggatg tcgagacttg tggcctcccc cgccgccagg
     2041 atgggtccac tcatgccggc tcctttcctt ttctcttctg acttgtcggt tcttctttcc
     2101 ttacgtagat ctgtcgagcg actttctctg agatcgttct ttcatggccg tccacgcgca
     2161 ggacgagcgg tccatcgaac ggatgtttct cgcggatctc gacgcgtacg cccggacgca
     2221 accccaaagc gtccaagaag tcgacgatct cttggtcggt agatccgggg accgccacta
     2281 ccgcgacgtc gcccggctcc aacgcgtaga gaggcggcat gtcggggatg tcgatgacat
     2341 ccgggtcggg gatcgggaat ccgtgtgggc agctctgtgg ccgatccagg gcgacgaaca
     2401 aacgatcttc gacctcttgc ggcagctcgt gctcgaaggt gccggcgagc cgatcggcct
     2461 cgttccacgc gtatccgagc atgtcggcga ggaatcgctc gacgatgcga tgccttcgga
     2521 tcgcgctgac ggccaaacgt cggcccgagc gcgtcagctc gacaccgcgg tagggcttgt
     2581 gggtcaagag gccccgctcg gccagccttt tcacggcagc cgtcgcggtc cccggagtga
     2641 caccgagcga ttccgcgagc gcgccggtgt gagccgccgg tacggggccg ccgcgggccc
     2701 cgcgagtcag gcggtagata gcctttagga actcgcgatc ggatttagag agtgtgccgt
     2761 ccatgattta gaaggcaggg aatctaactt ttggggcctc aaaaagtcaa atgaaagccc
     2821 tcagcgacgg aaagcgagat gggtgatcat gcccccaccg tcgcgcggct cgaagcgaca
     2881 ctcgtcgacg aggctctcca gaagggcgac ggagaacgcc agacgggacg agaacccctg
     2941 gctgtcctgc atgtcactgc cgcccagttg ctccacgctg ccggcgcggt cgctcacctg
     3001 aaccgtgatc gtggaaccgt cgtcgtcgta ctcgacgaga acggcctcct ccgaacctgc
     3061 ctcttcgtgc gccagtacgg agttgacgca cgcctcgccc acggcgatct tcagctcgtc
     3121 gacctgctcc tcgtccagcc cggaggcccg cccgagagcg gcaagggcaa gtcgaaccac
     3181 ccccacgtag ggggatcggg gagggatctc tacggatacg gcggccatcg gttaggccga
     3241 agctccggca gcctcctcga ctgagggatg gatgggaaac actttggtca atccggtgat
     3301 cttgaagatc ttcaggatct tgtcctgcgt gcagaccagc gccaggctgc cgtcgtgagc
     3361 cttcaccctc ttcagcgctc cgacgagaac ccccaacccc gtggagtcga ggaagtccac
     3421 tccctccaga ttcacgacga cgttgtagga gccgtccgag acgagctcga tcagcttctc
     3481 cctgagtttc ggcgctgtgt acacgtcgat ctcgcccttt acctcgacga cggcgtggtc
     3541 gccgacggtg gaaacgtcca gacccagctc catgttcccc ttccttaaat acctagggaa
     3601 attcggccgg tcatcctaaa ccggggttgt cgcgagccga tttgaactat ttagtgcctg
     3661 catagcaggc gggtgcccaa gcgtctaagt ggtatggata tccgtcattt tgggctccgg
     3721 cgtcgccata ctcagccgga gcgtagtcgg gagggcctga tgagctttgt cgtccccagc
     3781 caacgacggc tggtgtggcc gttagctgtt gcgctggcgg cagcagccgt gaccgggttg
     3841 gcgttgtatc tgccgaccag gtcgcatcac gtcccaacct cacccggagc ccccgccgtc
     3901 gtggccgcaa cggtcgccga ctgggagctg gcgatctacc ccgccggaag cgcaggcctc
     3961 ggcggggttt cgaagaaggc caaggcccga gttgaggccc agcggaaacc gctcgccacc
     4021 ctgatccaag atctttacga cgcgttgttc ctcgatccca cgaagctcga gaaagccatc
     4081 ggcatccatc tggagaaaac cgcgggcgcg gccctcatca aggatgcagg tctcccgaag
     4141 ggtctcgacg agctggagat cactactcgc aaagctcgag tgggaatctc ggtagcggga
     4201 gccgacaacg ccgcggccaa agtgaagatc accggcgtcg cgacccgcga cggtcgacgg
     4261 acgggcttca agcacgaatc cacactctgg ctcgagcgct cccacaaaga gtggaaggtc
     4321 atcgccttcg acctgaagca gggaccgaag agatgatccg cagagccact gtgctcgtca
     4381 ccctgctggc gagcctgata gcgacgcttt cgctggtggg cccggccggc acggccccac
     4441 tgctcgtggg cacgacccat agggcttacc aaccacagcg cgggaagatc ttcatcctcg
     4501 tcatcgggaa cgacgcgcgc tttggcaatc caccgacggc ccgcgccgac gcgacccacc
     4561 tagtcggcat caacacgaag acgatgaagg caggcatcct caacttccct cgcgactcgt
     4621 gggtgaacat cccgggccgc ggcatgtcca agatcaacga agccacccaa gcgggaggtc
     4681 cccagggact cgtggcgacg atggaaggac tgaccgggat caagatcgat tactgggtga
     4741 tgaccgggtt cgagggcttc atgggcatca tcgacggatt gggccgggtg cgggtccgaa
     4801 cgcatcagga cctttacgac ccgagcggat cggggatcca catgcccgcc ggtaagcaca
     4861 tgctcgggcc caagtcttca cttgcctatc tacgaactcg acact
//
LOCUS       DATMED010000301         6657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_994332, whole genome shotgun sequence.
ACCESSION   DATMED010000301 DATMED010000000
VERSION     DATMED010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994332"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VNP73_07695"
     CDS             <1..620
                     /locus_tag="VNP73_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL65841.1"
                     /translation="GSGRVDALKGIDASIPMGKVTAVVGPSGSGKSSLLRILAGLDRP
                     TEGSAEVAGTDVGRASAADLRRLRRRIGYVFQRPSDNLVSYLTVKEHVILAARLRKLS
                     EVDADEILHDLGLGARANHHPEQLSGGEQQRAAFAQAVAGDPPLVVADEPTAEIDSTT
                     AEALLSTIRKLADRGLTIVVATHDPAVMGVTDTSIRLHHGEVVGR"
     gene            617..1309
                     /locus_tag="VNP73_07700"
     CDS             617..1309
                     /locus_tag="VNP73_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL65842.1"
                     /translation="MTVLKVESVHKRFRRGTEIVHALEDVNLTLDAGEVCALVGPSGS
                     GKTTLLYVLAGWEIPDEGHVVSSAGDTGTASWEDIALIPQSLGLSEELTVRENVALPL
                     RLSERRVDEPPVDALLERLDLVDLADRYPLETSLGEQQRTAVARALVLQPNLILADEP
                     SSHQDDVRVDIIFGLLGEATAAGACCVVATHDPDVIRLVDRVVNMRDGRIERLIEGTA
                     ERGERALWGRPT"
     gene            complement(1306..1650)
                     /locus_tag="VNP73_07705"
     CDS             complement(1306..1650)
                     /locus_tag="VNP73_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65843.1"
                     /translation="MVSKPESAAPGAWAVVRGDEPRVFLAENEHVISRLLALQLVAKT
                     SPEEISSQVVLEEIRKSLLAEEWAVALATWIEETGSPVDVYPESLKVWTEADLDSDSA
                     SMEIRMAPIFAD"
     gene            complement(1640..3121)
                     /locus_tag="VNP73_07710"
     CDS             complement(1640..3121)
                     /locus_tag="VNP73_07710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWL65844.1"
                     /translation="MATKRNPTSTSNFGVGRRESHDATAFYSRFTMPGISNDDEVAPP
                     PVRAIDQIFCGDSRNMSELADSSVALVVTSPPYFAGKEYEQALGQGHIPGTYVEFIDM
                     LTDVFAECVRKLEPGGRIAINVANLGRKPYRSLSADVIAILQDRLGLLLRGEIIWQKA
                     YAASGSCAWGSFQSASNPVLRDLTERIIVASKGRFDRAVTRPKRLEAELPHKDSLFRD
                     EFMEATMDIWDMPPESATRVGHPAPFPVELPLRLIELYTYQGDLVVDPMMGSGTVAVA
                     ALKTNRHFACYDTDQSYVDAARERVEKVRRELLEERGPAPAGQFVPNIPLPIGKDVHH
                     QSRAVQEGQRAKEIARAVLKDCKFKNIQEERRFGNGVEVNFVAKDAKGQKWFFDVSGA
                     FTSTRAGLKRTDTVWKALGRAATLKASPKVDKYRLVFLTTDLPPRGSAGNQALSATRG
                     DLFHDAIEMLSKTGQSRLRMYAAGEHYDEPIGQLLSSTEGDGE"
     gene            3245..4474
                     /locus_tag="VNP73_07715"
     CDS             3245..4474
                     /locus_tag="VNP73_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65845.1"
                     /translation="MKLRVFAVAGAVALAASVVVAPSAQAQNVIDVQMSRPLGKGLRD
                     AFSVRPGAPIVDGVPTIEVARDGIVHLVDSPAILLPEGMAAMDWVDQYARGSDAPWAP
                     IVADPDGDLPGLNAPSRLNIPAFDPTVEECGASAADPCVFDGSNPDPIEGVMSSGDIV
                     SEFFVQITAQPNATIDVIPLVLASDELVSLRIRVIAQEGEATTQAELDGAAQDVRAQE
                     RATALEIKKEINSVSRTREGDHWVYDAFGGYDTETIILFGFYPRKLQIKKGDRVVWHF
                     PLALEAHTASFPFKKGLKAGNRGFAFACDPDGSGEGPDTPPGRSEEDPCNPGEPELDL
                     SRKITAQAGDGQFPGGSNKYENSGLKAGWLPTDIDGVKGGTEDWALAFNARSSDKGFK
                     YICIFHGRGMTGKVIVK"
     gene            complement(4512..5105)
                     /locus_tag="VNP73_07720"
     CDS             complement(4512..5105)
                     /locus_tag="VNP73_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65846.1"
                     /translation="MPRGRRPEDWEKDLAQARRAEADLAQILAGDSRLDDFLDHTMDY
                     DKLDFSFAYAGLKVQLDLKEKRQRYSPDYQTHWPEVALEDLFIIDETVYRRIVWQGGG
                     GYLAVHDHPSGRWCYFGPWELTLGPQVRYQRWGKRSGRSFLKGKILLDLNSAAYVGRN
                     FVVDDLLRVILRSAEALGSVEAVAINGYPTQEVGKYA"
     gene            5215..5532
                     /locus_tag="VNP73_07725"
     CDS             5215..5532
                     /locus_tag="VNP73_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65847.1"
                     /translation="MRFERHSKSMESLREHLENMSTVVEHQRKKNGNGDTHPLSKRTG
                     EKRTLRECVAVMIWEDEDGTETVSVVSDPGITDLEVKGVLHDAVYAVAHVGEEGFNTV
                     AIQ"
     gene            complement(5544..5957)
                     /locus_tag="VNP73_07730"
     CDS             complement(5544..5957)
                     /locus_tag="VNP73_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWL65848.1"
                     /translation="MAVDPRGLAECFFDAFNRGAIDEAVTYFADNCSHHEPVRGRITR
                     PQFKANLEGLKRAFPDGKMNVESAVIRGRTVAVEGHFTGTHSGLLSGPNVEFSPTDRP
                     IKIRFANFFTVERGKIVSHRVYYNQLDVVTQLRLL"
     gene            6072..>6657
                     /locus_tag="VNP73_07735"
     CDS             6072..>6657
                     /locus_tag="VNP73_07735"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HWL65849.1"
                     /translation="MTDLPGTDETSFEELLGLGTPLYTSAEISKRAGIPQDEAQRIWR
                     AMGFVEPAEDTKHFTEADLELLVRVVDFMKEGFADLELILGMTRVISRSVGLLADAEA
                     DAIHSTVVQNPSLLEEIDEKGLDSIAPRVFDDLEAFLVHVWRRHLGDALARRNLEELP
                     GREEPMAVGFADLVGFTRLSRYIDGTELAALVEKF"
BASE COUNT         1248 a         2104 c         2107 g         1198 t
ORIGIN      
        1 ccggatcggg gcgggtggat gcgctcaagg gcatcgatgc ctcaatccct atgggcaaag
       61 tcactgcggt cgtcggcccg tcgggaagcg gcaagtcgtc gctgttgcgg attctcgcgg
      121 gactcgatcg cccgaccgag ggcagcgcgg aagtcgcggg aaccgatgtg ggccgcgcat
      181 cggcggcaga cctccgtcgt ctgcgccgca ggatcggtta cgtcttccaa cgcccttccg
      241 acaacctcgt ctcgtacctc acggtgaagg agcacgtgat cctcgccgcg cgcttacgca
      301 agctgagtga ggtagacgcc gacgagatac tccacgatct cggattggga gcgagagcga
      361 accatcaccc cgaacagctt tccggcggag aacagcagcg ggcggcgttc gcacaagcag
      421 tcgccggcga tcccccactc gtggtagccg acgagcccac ggccgagatc gattcgacaa
      481 ccgccgaggc gctcctgtcg acgatccgga agctggccga caggggactc acgatcgtgg
      541 tcgcaacgca cgaccccgcg gtgatgggcg ttaccgacac ctcgatccgt ttgcatcacg
      601 gcgaggtggt cgggagatga ccgtgctcaa agtggagtcg gtccacaagc gattccggcg
      661 cggaaccgag atcgtgcacg cgctggagga tgtgaatctc acgcttgatg ccggcgaagt
      721 atgtgcgctg gtggggccgt ccgggtccgg gaagaccaca ttgctgtacg tcctcgccgg
      781 ctgggagatc cccgatgagg ggcacgtggt gtcgagcgcc ggtgacaccg gcaccgcaag
      841 ctgggaagac atcgcgctca tcccgcagag cctcgggctt agcgaagagc tgaccgtgcg
      901 cgagaacgtc gctcttccct tgcggttgag cgaacgtcgt gtagacgagc ccccggtcga
      961 tgccttgctc gagcgattgg atcttgtcga tcttgcagac cggtatccct tggagacttc
     1021 actcggcgag cagcagcgca ctgcggtggc gcgcgctttg gtgctgcaac ccaacctcat
     1081 cctcgccgac gagccctcca gccatcagga cgacgtgcgc gtcgacatca tcttcggcct
     1141 cctcggcgaa gctacagcgg ccggagcctg ttgcgtcgtc gcgacgcacg accccgacgt
     1201 gatacgactc gtcgatcgtg tcgtgaacat gcgcgacggc aggatcgaga ggctcatcga
     1261 gggcacagcg gagagaggcg agcgggcgtt gtggggtcga ccgacttagt cggcgaagat
     1321 cggcgccatc cggatctcca tggaggcgct gtcactatcg aggtccgctt cggtccacac
     1381 cttgagcgat tcggggtaga cgtcgacggg cgagccggtc tcttcgatcc aggtcgcaag
     1441 ggcgaccgcc cattcctctg caagtaatga ctttcggatc tcctccagca cgacttggga
     1501 cgagatctct tccggcgaag tcttggccac gagttgcaac gccaggagtc gggagatgac
     1561 gtgctcgttc tcggcgagaa agaccctggg ctcgtcccca cggaccacgg cccatgcccc
     1621 gggcgccgcc gactcgggct tactcaccat ccccctcggt ggaagaaagc agttggccga
     1681 tcggctcgtc gtaatgctcg ccggcggcgt acatccgcag tcggctttgg cccgtcttgg
     1741 acagcatctc gatcgcatcg tggaataagt ccccgcgcgt ggcgctcagg gcttggttcc
     1801 ctgcgctgcc ccgtggtgga aggtcggtcg tcagaaacac gagcctgtac ttgtccacct
     1861 tcggcgaggc cttgagcgtg gctgcgcgtc ctaacgcttt ccagacggtg tcggttcgct
     1921 tcaaaccggc gcgcgtgctg gtgaatgcgc cggacacgtc gaagaaccac ttctggccct
     1981 tggcgtcctt cgcgacgaag ttcacctcga cgccgttacc gaagcgacgc tcctcctgga
     2041 tgttcttgaa cttgcagtcc ttgagcaccg cccgggcgat ctctttggcg cgttggcctt
     2101 cctggaccgc tcgcgactga tggtgcacgt ccttaccgat cggcagcgga atgttgggta
     2161 cgaactgtcc cgcgggagcc ggtccccgct cctcgagcag ttcgcggcga accttctcaa
     2221 cgcgttcgcg cgccgcgtcg acataactct gatccgtgtc gtagcaggcg aaatgacgat
     2281 tcgtcttgag ggcggcaacc gccaccgtcc ccgagcccat catggggtcg acgaccagat
     2341 cgccttgata ggtgtaaagc tcgatcaatc gcagaggcag ctcgaccggg aaaggagcag
     2401 gatggcctac ccgggtcgca ctctccggcg gcatgtccca aatatccatc gtcgcttcca
     2461 tgaactcgtc gcggaacagg gagtccttgt gaggcagctc ggcttccaag cgtttgggcc
     2521 gggtgaccgc ccgatcgaag cgccctttcg aggccacgat tatccgttcg gtcaggtcgc
     2581 gcagaaccgg gttggatgcg ctttggaacg agccccacgc gcacgatcca ctcgcggcat
     2641 aggccttttg ccagatgatc tcgccgcgca gcaagagtcc tagacggtcc tgcaggatcg
     2701 cgatcacgtc tgcagatagc gagcgatacg gcttccgccc gaggttcgca acgttgatcg
     2761 cgatccggcc ccccggctcg agcttgcgaa cgcattcggc gaacacatcg gtcagcatgt
     2821 cgatgaactc gacgtatgtg cccgggatat ggccctgacc gagcgcctgt tcgtattcct
     2881 tgccggcgaa gtacggaggg gacgtgacaa cgagagcgac cgaggaatcg gcaagctcgg
     2941 acatgttgcg ggagtcgccg cagaagatct ggtcgatcgc gcgtaccggc ggaggggcga
     3001 cctcgtcgtc gttggagatc ccgggcatag tgaagcgcga gtagaaggcc gtcgcgtcgt
     3061 ggctctcgcg cctaccgacg ccgaagttcg aggtcgatgt ggggtttctc ttggtggcca
     3121 tttcggctcc taccttaaag acggggtacg acagttttcc ggacctcggc gacccccctg
     3181 gacagggccc ccctccgcgt ggctgaatct caccgccggt atttccagtt gagggaggaa
     3241 atctatgaag ttgcgggtct ttgccgtcgc gggcgctgta gccctggcgg cgtcggttgt
     3301 ggtcgccccg agcgcccagg cccagaacgt gatcgacgtc cagatgtcac gccccctcgg
     3361 caaggggctg agggatgcgt tctcggtccg cccgggcgcc ccgatcgtcg acggcgtccc
     3421 caccatcgag gtggcccggg acggcatcgt ccacctcgtg gacagcccgg ccatcctgct
     3481 gccggaaggc atggccgcga tggactgggt cgaccagtac gcccgcggtt cggatgcgcc
     3541 gtgggctccg atcgtggccg accccgacgg ggacctgccc ggcttgaacg ccccgagcag
     3601 gcttaacatc cccgcgttcg accccaccgt cgaggagtgt ggagcgtccg cagccgaccc
     3661 gtgcgtgttc gacggttcga accccgaccc gatcgaggga gtcatgtcct cgggcgacat
     3721 cgtcagtgag ttcttcgttc agatcaccgc gcagccgaac gcaacgatcg acgtcatccc
     3781 gttggttctc gcctcggacg agctcgtaag tctccgcatc cgtgtcatcg cgcaggaagg
     3841 cgaggccacg acgcaagccg agttagacgg agctgcacaa gatgtgcgtg cccaagaacg
     3901 ggcgacggct ctggagatca agaaagagat caacagcgtg tcgcgcacgc gtgagggcga
     3961 ccattgggtg tacgacgcgt tcggtggcta cgacaccgag acgatcatcc tcttcggctt
     4021 ctacccgagg aagcttcaga tcaagaaggg tgaccgggtc gtgtggcact tccctctggc
     4081 gctcgaggcg cataccgcgt cgttcccgtt caagaagggt ctgaaagccg ggaaccgcgg
     4141 tttcgccttc gcctgcgatc cggacggcag cggtgaagga cccgacacgc ccccgggtcg
     4201 ttcggaggaa gatccgtgca acccgggcga gccagagctg gatctgagtc gcaagatcac
     4261 tgcccaggca ggcgacggtc agttccccgg cggatcgaac aagtacgaga actctgggtt
     4321 gaaagcaggt tggctgccca ccgacatcga cggcgtcaag ggtggcactg aggattgggc
     4381 gctagcgttc aacgctcgtt cgagcgacaa gggcttcaag tacatctgca tctttcacgg
     4441 ccgcgggatg accggaaagg tcatcgtcaa gtaacgaacc gcaacgcttt gtaactatga
     4501 ggtcgggaag gtcacgcgta cttcccgacc tcttgcgttg ggtaaccgtt gatcgctact
     4561 gcctcgaccg agccgagagc ttcggcagac cgcaggatca cccgtaagag gtcgtcgacc
     4621 acgaagttgc gccccacata ggcggccgag ttcaagtcga gcaggatctt gccctttaag
     4681 aagctgcggc ccgagcgctt gccccaccgc tggtaccgaa cctggggacc gagggtcagc
     4741 tcccacggcc cgaaatagca ccatcgcccc gaggggtggt cgtggacggc caggtacccc
     4801 ccacccccct gccacacgat ccgccggtag acggtctcgt cgatgatgaa cagatcctcg
     4861 agcgccacct ccggccagtg ggtctggtag tcgggcgaat aacgctggcg cttctccttc
     4921 agatcgagct gaaccttgag ccccgcatag gcgaacgaga aatcgagctt gtcgtagtcc
     4981 atcgtgtgat cgaggaaatc gtccaaacgg gagtcccctg ccagaatctg ggccaggtcg
     5041 gcttccgccc gccgcgcttg ggcgaggtcc ttctcccagt cttccggtcg tcgtccccga
     5101 ggcatcgaat caggataact aggccagaac cctccaagcg ggctataggc aaaccaatcc
     5161 ccctctccta acgtccttgg agagcgtgcc gacggcgaaa tggggaggaa ccgaatgcgt
     5221 ttcgagcgac acagcaagtc gatggagtca ctgcgcgagc atctcgagaa catgtcgacg
     5281 gtcgtcgagc accaacgcaa gaagaacggg aacggcgaca ctcatcccct gtccaaacgt
     5341 acaggtgaaa agcgcacgct gagggaatgc gtcgcggtga tgatctggga agacgaagac
     5401 ggaaccgaaa ccgtctccgt agtgagcgac cccgggatca ccgacctcga ggtcaaaggc
     5461 gttctgcacg acgccgtcta tgcggtcgcg cacgtcggcg aagagggctt caacacggtc
     5521 gcgatccagt agcaggaccc ccgctacaac aaccgtagct gcgtaacgac gtcgagctgg
     5581 ttgtaataga cgcgatgcga gacgatcttt cctcgctcga ccgtgaagaa gttcgcgaag
     5641 cgaatcttta tcggccgatc cgtcggcgag aactcgacgt tcggaccact cagcagaccg
     5701 ctgtgagtgc ccgtgaagtg gccctccacc gcgaccgttc ggccccggat cacggccgat
     5761 tccacattca tcttcccgtc gggaaaggct cgcttgagcc cttcgagatt ggccttgaac
     5821 tgagggcgcg tgatgcggcc tcggaccggt tcgtggtgac tgcagttatc ggcgaaatag
     5881 gtgactgcct cgtcgatggc gcctctattg aaggcgtcaa agaaacactc ggccaagccc
     5941 cgcggatcaa ccgccaattg acagctcctc cgatcgagag atagctcccc tattcccgat
     6001 tgcttgaggt gattcgacgc gtaccaggct tgccctgctt gcccaccgaa atcggtcgct
     6061 aacctgaggg catgaccgac ctgcccggca cggacgagac ctcgttcgag gagctcctcg
     6121 gcctcgggac gcccctgtac acgtccgccg agatctccaa gagggccggg atcccccagg
     6181 acgaggccca gcggatctgg cgagccatgg gcttcgtaga gccggccgaa gatacgaagc
     6241 acttcaccga agccgacctc gagcttcttg tccgcgtcgt ggacttcatg aaggagggct
     6301 tcgccgacct cgagctgatc ctcggaatga ctcgagtcat cagtcgctcg gtggggttac
     6361 tcgccgatgc tgaagccgac gcgatccatt cgaccgtcgt gcagaacccg tctctgttgg
     6421 aagagatcga cgagaagggt ctcgattcga tcgcgccgcg cgtattcgac gacctggagg
     6481 cattcctcgt ccacgtgtgg cgccgccacc tgggagacgc tctcgctcga cgcaacctcg
     6541 aagagcttcc cgggcgggag gagccgatgg ccgtgggctt cgccgacctg gtcggcttca
     6601 cacgcctgag ccgctacatc gatggaaccg agctggccgc actcgtcgag aagttcg
//
LOCUS       DATMED010000302         4847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4845709, whole genome shotgun sequence.
ACCESSION   DATMED010000302 DATMED010000000
VERSION     DATMED010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4845709"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VNP73_07740"
     CDS             <1..857
                     /locus_tag="VNP73_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWL65850.1"
                     /translation="LLQDEFRRRLQWIRDAILRETRMRTARIRDPDYVARYVVDPVPE
                     ETNFFSAATDAAELRRAVRPLARKLATRVAMKRRRARRGHIDMRRTMRHSLSTGGVPI
                     ETPFKHRTPHRPELLLLCDVSSSVARFSRFTLMLTHALRAQFQKVRTYAFINTLADIT
                     DQLQVEDFGEAMESIGSEAAVDGYDGRTDYGTVFRELAEDHGDAFGPKTTVLVLGDAR
                     SNYRARYVDHLKAVRKQVRHIYWLNPEPVSDWDIGDSAASEYAACVDKMVEVRNLRQL
                     QEFIATEL"
     gene            complement(861..1079)
                     /locus_tag="VNP73_07745"
     CDS             complement(861..1079)
                     /locus_tag="VNP73_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65851.1"
                     /translation="MSTNDRSYALQVVGSLIVAALIVWITVAVVASQLPLRYLPEEQT
                     EELQEEAEERREERQERIEERREEKAED"
     gene            complement(1076..1525)
                     /locus_tag="VNP73_07750"
     CDS             complement(1076..1525)
                     /locus_tag="VNP73_07750"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HWL65852.1"
                     /translation="MDDLIEELTRGHVTEVKIVLTSIVAALAVYQVLLMTVGYGKVRL
                     PFLKPPAASFAHRASGDMIVTLTMLVGLMCLGYFGIEDGIEHARPGQRDVVALHVVLG
                     FLLAGAIVFKVIVVRWWHAMGRFLPLIGISVLLLFLLTWWTSAGVYL"
     gene            1616..2101
                     /locus_tag="VNP73_07755"
     CDS             1616..2101
                     /locus_tag="VNP73_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HWL65853.1"
                     /translation="MDPLMIIVTSLLMVIGLLGVFIPVLPGLFLIVLAGVLYGWATDF
                     GLIGSIVLGVMIVMAVLGTAAGYVLPHKRMAETGAPRSTILAGLILGVVGFFVVPIIG
                     FLIGVIAGVLLAEKSRTGDWSTARSSTRTLLVGFGLGKLFELTAGLAMVGLWVGWVFS
                     Q"
     gene            2177..4450
                     /locus_tag="VNP73_07760"
     CDS             2177..4450
                     /locus_tag="VNP73_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65854.1"
                     /translation="MRRRWAAAILAVILVILTAGPALATPVCTDGYMGGPPRDLCGGR
                     VFPEVENSVDYVQFLPDPAGFSEYRHGLEYLEKLYPRWISVFTLAKHYDDKDAVSAGP
                     DAVRSFEKEDSGDGHDIVVVKLTDHNVPDKGKQNLLFSLSIHGDEKGGIEGGVRAVED
                     LAIAADSGDGTIVDGVKGYESTTGKKPKFHEYPVRQVLAEEAVYFVDFNPDGWTAGDH
                     ASDGAFLYTRQNHMGTDLNRQMPTIGYIDPSRNPLEESEMNYGHRFMHEVAAAGVGGK
                     MAYGADVHGEGQSRAWVDIMYPAGQFNSIKHRQHMAIAERTKSVIDATLFNGSVDEGE
                     EATGGDGGEGAEDSGAPPNVVPTKPARWGTVWDTLGYTDTGFLGDYLANELGVTGMDY
                     EIAFNHSDFRAYGRPWTQFFQENYVNATRAIVKTAMAYALYQTKEFNARNVRIDPKGR
                     VGYVFNPEVVTHADGVGTKAGPGGDGVSANGEPVEQAPYRATNMAFFKEESKLVVGGF
                     NKAHPADIAKSKSYLNRFDSLVLADLTAPKDAKGRTYKKKAYFANLKAWVKSGGNLVL
                     TDRGLHVLGSIGVVPKDSVTNIQVYQPFSNFQDFEHPMLSGLRKNARQLSEATLVGYG
                     IGNTASPMSVVATAAWEEAGGHVVGTTSAGSLVDSDDGSQTSVGELKFGKGKIRIMGG
                     GLGMPTEKNDHRYGLKDYALTYSGLYIIENSIVHDAPDLGPKKGALRPGSSLNLGLLL
                     LPVGAAAAFVRRKRRMS"
     gene            4521..>4847
                     /locus_tag="VNP73_07765"
     CDS             4521..>4847
                     /locus_tag="VNP73_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWL65855.1"
                     /translation="MASGAAEEFGEFVSAQEVLAAIVTSADDAIYSKDRDAVIRSWNP
                     AAERLYGYKPEEILGRSVALLIPEDHPNEEMVILQRILAGSVVEHYETVRKCKDGTLV
                     PVSLMVS"
BASE COUNT          974 a         1421 c         1543 g          909 t
ORIGIN      
        1 ggttactcca ggacgagttc cgtagacggc tgcagtggat ccgagacgcg atcctgcgcg
       61 agacgaggat gcgaacggcg cggatccgcg acccggatta cgtcgccagg tacgtcgtgg
      121 atcccgtccc cgaggaaacg aacttcttct cggcggcaac ggatgcggcc gaactcaggc
      181 gagccgtgcg tccgctcgcg cggaagctcg cgacacgcgt cgcgatgaag aggagaaggg
      241 cgagacgagg gcacatcgat atgcggagga cgatgaggca ctcgctgtct accggcggag
      301 tgccgataga gacgcccttc aagcatcgga cccctcaccg ccccgagctg ctgcttctct
      361 gcgatgtgtc gtcctcggtg gcccgattct ctcgattcac gctgatgctg acgcacgcgc
      421 tgcgggccca gttccagaag gtgcggacgt atgccttcat caacacgctt gccgacataa
      481 cggatcagct acaggtcgaa gacttcggag aggccatgga atccatcgga agcgaagcgg
      541 cggtggacgg ttacgacggc cgtaccgatt acgggacggt tttcagggag ttggccgaag
      601 accatggaga cgcgttcgga cccaagacca cagtgctcgt cctgggcgac gcccgcagta
      661 actaccgggc gagatacgtg gaccacctga aggcggtgag gaagcaggtc cgacacatct
      721 actggctcaa tcccgaaccg gtttcggatt gggacatcgg ggattcggca gcatcggaat
      781 acgcggcctg cgtggacaag atggtcgagg taaggaatct ccgacagctg caggagttca
      841 tcgccaccga gctgtaaggg tcagtcctcg gccttctcct cgcgacgttc ttcgatccgt
      901 tcttgccgtt cctccctgcg ttcttcggct tcttcctgca gctcttcggt ctgttcctcg
      961 ggcagatacc tcagggggag ctgggaagcg accaccgcga ccgtgatcca gacgatgaga
     1021 gcggcaacga tcagcgatcc aacgacctgc agcgcatagg agcggtcgtt cgtgctcata
     1081 ggtagacccc agcagacgtc caccacgtga gcaagaacaa caagagaacc gagatcccga
     1141 tcaaggggag gaagcgtccc atcgcatgcc accagcgcac gacgatgacc ttgaacacga
     1201 tggctcccgc aagcaagaaa ccgagaacca cgtgaagcgc cacgacgtca cgttgtccgg
     1261 gacgggcatg ctcgatcccg tcttcgatcc cgaagtagcc gagacacatc aaccccacga
     1321 gcatcgttag cgtcacgatc atgtccccac ttgcccgatg tgcgaacgag gcggccggcg
     1381 gtttcaggaa gggcagccgc acctttccgt aacccacggt catcagaagc acttggtaca
     1441 cggcaagggc cgcgacgatc gaggtcaata cgatctttac ttcggtgacg tgaccgcgag
     1501 tcagttcctc gatcagatcg tccacagcgc gcgaccctaa gcccgctggg cagtgggcgg
     1561 gaggtactga ttggacgggc gaagcccgtt cccccagtag tggagacttc gcaatgtgga
     1621 cccgctgatg atcatcgtga cctctctgct catggtgatc ggcctactcg gtgttttcat
     1681 ccccgtctta cccggcctgt tcttgatcgt gctcgcgggg gtgctgtatg gatgggcgac
     1741 tgacttcggg ctgatcggct cgatcgtcct cggtgtgatg atcgttatgg cggtcctcgg
     1801 caccgccgcg gggtacgtgc tgccgcacaa gcggatggcc gaaaccggtg cgcccaggtc
     1861 gacgatcttg gccggcctga tcttgggcgt ggtcgggttc ttcgtcgttc caatcatcgg
     1921 gttcttgatc ggtgtgatcg cgggggtcct gctggccgag aagtcgcgta ctggtgactg
     1981 gtcgacggcg cgctcgtcga cgagaacgct cctcgtcggc ttcgggctgg ggaagctgtt
     2041 cgagctcacg gccggactgg cgatggtggg gctctgggtg ggatgggtgt tctcccagta
     2101 gccctacgct agaggagcag gggagcttcc cggcgaatct ttatggcgga aaccgaactc
     2161 tcgaaaggcc tgacacatgc gacgtagatg ggcagccgcg atcctcgcag tcatcctcgt
     2221 gatactgacc gccggtccgg cgctggccac accggtgtgc acggacggct acatgggtgg
     2281 tcctcctcga gatctgtgtg ggggccgcgt gttccccgag gtcgagaact cggtggacta
     2341 cgttcagttc cttcccgatc cggccggttt cagcgaatac cggcacggac tggagtatct
     2401 cgagaagctc tacccacgct ggatctcggt tttcaccctg gccaagcact acgacgacaa
     2461 ggatgccgtc agtgccggcc ccgatgcggt gcgctcgttc gagaaagaag actcgggaga
     2521 cggtcatgac atcgtggtcg tgaaactcac cgaccacaat gtgcccgaca aaggcaagca
     2581 gaacctgctc ttttcacttt cgatccacgg tgatgagaaa ggtgggatcg agggtggtgt
     2641 tcgcgcggtc gaggaccttg cgatcgcggc cgactcgggt gacggaacga tcgtcgacgg
     2701 cgtgaagggt tacgagtcga cgaccggcaa gaagccgaag ttccacgaat atccggtccg
     2761 tcaagtactg gccgaagaag ccgtttattt cgtcgacttc aaccccgacg gttggacggc
     2821 gggggaccac gcatcggacg gtgccttcct ctatacgcgc cagaaccaca tgggcaccga
     2881 cctcaaccgt cagatgccga cgatcgggta catcgatcca tcccgtaatc cactcgagga
     2941 aagtgagatg aactacggac atcgcttcat gcacgaggtt gccgccgcag gtgtgggcgg
     3001 caagatggcg tacggggcgg acgttcacgg tgaaggtcag tcgcgcgctt gggtcgacat
     3061 catgtacccc gccggccagt tcaactcgat caagcacagg cagcacatgg cgatcgccga
     3121 gcgcaccaag tcggtgatcg acgcaacatt gttcaacggc tcggtggacg aaggcgaaga
     3181 ggccaccgga ggcgacggtg gcgagggcgc ggaggattct ggtgcccccc cgaacgtggt
     3241 cccgacgaag cctgctcgat ggggaaccgt gtgggacacc cttgggtata cggacaccgg
     3301 cttcctcggc gattacctag cgaacgagct cggtgtaacg ggcatggact acgagatcgc
     3361 cttcaatcat tcggacttcc gcgcctacgg tcgcccgtgg acccagttct tccaggagaa
     3421 ctacgtcaac gcgacgcgcg cgatcgtgaa gactgcgatg gcgtacgccc tgtaccagac
     3481 gaaggaattc aacgcgcgga acgttcgcat cgatccgaag ggtcgggtcg gctacgtgtt
     3541 caacccagag gtcgttaccc atgccgacgg tgtcggaacg aaagccgggc cgggtggcga
     3601 tggagtgagc gcaaatgggg agcccgttga gcaggctccc taccgcgcta ccaacatggc
     3661 gttcttcaaa gaagagagca agttagtcgt cggcggattc aacaaagctc atcccgccga
     3721 catcgcaaag agcaagagtt acctgaaccg gttcgactcg ctcgtgctgg ccgacctgac
     3781 ggctccgaag gatgccaagg gccgcactta caaaaagaag gcgtacttcg ccaacctcaa
     3841 agcgtgggtg aagagcggag gcaacttggt tctcacagac cgtggcctcc acgtattagg
     3901 gtcgatcggc gtcgttccca aggattcggt cactaacatc caggtctacc aaccgttctc
     3961 caacttccaa gatttcgagc atccgatgct ctcggggttg cggaagaacg ccagacaatt
     4021 gtccgaggcg actctcgtcg ggtacgggat cggcaatacc gcgtctccga tgtctgtcgt
     4081 cgcgacggct gcatgggagg aggccggtgg tcacgtcgtg gggacgacga gcgcgggttc
     4141 cctcgtcgat tccgacgatg ggtctcaaac gtcggtcggc gagctcaagt tcggtaaggg
     4201 caagatccgc atcatgggtg gcgggctcgg tatgccgacc gagaagaacg accaccgtta
     4261 cgggttgaag gattacgcgc tgacctactc gggcctctac ataatcgaga actcgatcgt
     4321 tcacgatgct ccggatctgg gacccaagaa gggcgcgctg cggcctggtt cgtcgttaaa
     4381 cctcggtctg ctgctgttac cggtcggagc tgccgcggcg ttcgttcggc gcaagaggcg
     4441 gatgtcctaa gagggaagat tgtccgactc tggtgattgg atcgacaggc ctttgttcct
     4501 gagtcgctac cctgttgccc atggctagcg gggcggccga agaattcggc gagttcgttt
     4561 cggcacagga ggttctcgcc gcgatcgtca cgtcggcgga cgacgcgatc tactcgaagg
     4621 atcgcgacgc ggtcatacga agttggaacc ccgctgcgga gcggctgtac ggctacaagc
     4681 cagaagagat tctgggtcgg tccgtcgcgt tgctgatccc cgaggaccat cccaacgagg
     4741 agatggtcat ccttcagcgg atcctcgccg gaagcgtcgt cgagcactac gaaaccgttc
     4801 gcaagtgcaa agacggaacc ctggtccccg tctccttgat ggtctcg
//
LOCUS       DATMED010000303         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4088906, whole genome shotgun sequence.
ACCESSION   DATMED010000303 DATMED010000000
VERSION     DATMED010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4088906"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VNP73_07770"
     CDS             complement(<1..829)
                     /locus_tag="VNP73_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWL65856.1"
                     /translation="MDILANMWNGFWNTCPFEFCRWDFMQKALVAAALVGVTAPAIGI
                     FLVQRRLSLMGDGIGHVAFTGVAAGFLLDTLPLVTAIVAAILGALAIELMRERGRTEG
                     DVALAVVFYGGIAGGALLMGLSETSNTNILTYLFGALLSVTRSDILAALGISVVVLGI
                     ATLLRRVLFAVSYDEEVARASGVSVRTLNLLVAVAAAITVGVTMRVVGILLVSGMLVL
                     PVAAVQQLTRSFNATFAGSLVLGLLISVAGVVTSYYVDAPPGATIVMAAIAAFVLASF
                     "
     gene            complement(829..1590)
                     /locus_tag="VNP73_07775"
     CDS             complement(829..1590)
                     /locus_tag="VNP73_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL65857.1"
                     /translation="MTPDFHLSGATIHFGATVALRNVHFHLNRGEFVVLLGANGSGKT
                     TMVKALLGLIPLRKGRLDVFGTPLPKFRDWGRIGYVPQRASAASGVPASAFEVVLSGR
                     LARSRKWGPHSKDDARAVREALELVDMADKHKEPVGNLSGGQQQRVLIARALAGEPDV
                     LVLDEPVSGIDLEHQESFATTLGELNRGGRSVLLVAHALGVIEPLVSRAVVLTQGEVS
                     YDGKPLHQLHDSHVHHHPHSAEQALPFSNPAGGSH"
     gene            complement(1587..2471)
                     /locus_tag="VNP73_07780"
     CDS             complement(1587..2471)
                     /locus_tag="VNP73_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWL65858.1"
                     /translation="MRPIVLALVALVMLAVGGCGRAGGESGTSEGPKVIAAFYPLEFV
                     ATEIVGRNATVTNLSPPGVEPHDLELAPDQVRSLAQADLIVYIGQGFQPAVEDVIGEL
                     SGTRMVDALDTQDELIEAGAADPHVWLDPTRLASLGNVIAREMVEIEPGNADVYRDNA
                     ATLAGALNELDEEYSAALADCERNELVVSHEAFRYLTDRYGLEQVGVSGIDPESEPSP
                     GRVAEVAEFARDHGVTTIFFEEQVAPDIAQVIADEIGARVEVLDPLEFKPEGDSDYLD
                     VMRSNLDSISAALGCNRS"
BASE COUNT          525 a          806 c          796 g          441 t
ORIGIN      
        1 agaaactggc aaggacgaag gcagcgattg ctgccatcac gatcgttgca ccgggcggcg
       61 cgtcgacata gtaggaggtc acgactccgg cgacgctgat caacagtcct agtacaaggc
      121 tgccggcgaa cgtcgcgttg aagctccgcg tcaattgctg cacggccgcc accgggagca
      181 cgagcatgcc ggacacgaga aggattccga cgacccgcat ggtcactccg acggtgatgg
      241 ctgcggccac cgccactaac aggttcaagg tgcgcacgga gactcccgac gcacgagcca
      301 cctcctcgtc gtaagagacg gcgaagagaa cacgtcgcag cagcgtggcg atccccagaa
      361 cgacaacaga gatgcccagc gcggccagga tgtcagaccg agtgaccgaa agcagcgctc
      421 caaacagata ggtcaagatg ttggtgttcg aggtttcgga taagcccatg agcaatgctc
      481 ctcccgcgat gcccccgtag aacaccaccg caagggcaac gtcgccctcg gttcggccgc
      541 gttcccgcat caattcgatg gcgagagcac ccagaattgc ggcgacgatc gcggtgacca
      601 gcggcagcgt gtccaacagg aagccggctg caaccccggt gaatgcgacg tggccgatcc
      661 catcacccat cagtgacaag cgccgctgga cgagaaagat gccgatggcg ggggcggtca
      721 ctccaacaag cgcggcggca acgagcgcct tctgcatgaa atcccatcgg cagaactcga
      781 acgggcaggt gttccaaaac ccgttccaca tgttcgccag gatgtccatc agtgcgatcc
      841 cccggccgga ttcgagaagg gaagggcctg ctcggccgag tggggatgat gatgaacgtg
      901 actgtcgtgc aactggtgta gcggttttcc gtcgtaggac acttcgccct gcgttaggac
      961 cacggcgcgc gacaccaatg gctcgatgac gccaagcgcg tgcgcaacga gcagaacgct
     1021 gcggcccccc ctgttcagtt cgccgagcgt cgtcgcgaag ctctcttggt gctcgagatc
     1081 tattcccgag actggttcgt cgagtaccag cacgtccggc tcgcccgcca aggcccgcgc
     1141 gatgagtact cgctgttgct gaccccccga aaggttcccc acgggttcct tgtgcttgtc
     1201 ggccatgtcc acgagctcga gcgcttccct taccgcgcga gcgtcatcct tgctgtgcgg
     1261 cccccacttt ctcgacctgg caagacgacc agacaggacg acctcgaaag cgcttgccgg
     1321 gacgccagag gccgcgctcg cgcgctgggg cacgtagccg atccgacccc aatcacgaaa
     1381 ctttggcagg ggcgttccaa aaacgtcgag acgacccttc cgaagaggga tgagacccag
     1441 cagggccttg accatggtcg tcttgcccga accgttggct ccgaggagca cgacgaactc
     1501 gccgcgattg agatgaaagt gcacgtttcg caaagccact gttgctccga agtggatggt
     1561 tgccccagag agatgaaagt cgggtgtcat gacctattgc acccgagcgc tgcggagatg
     1621 gaatccaagt tggaccgcat gacgtcgagg tagtccgagt cgccttcagg cttgaactcc
     1681 aatggatcga gaacctctac gcgcgcgccg atctcgtcgg caatcacctg agcgatgtcg
     1741 ggcgcgacct gctcttcgaa gaagatcgtc gtcacaccgt gatcgcgtgc gaactcggcg
     1801 acttcagcga cgcgcccggg cgacggctcc gattccggat cgattcccga tacaccgacc
     1861 tgctcgagcc cgtaacggtc ggtgagatag cggaacgctt cgtggctcac gacgagctcg
     1921 tttcgttcgc agtcggcgag agcggccgag tattcctcgt ccagttcgtt caacgcgccc
     1981 gcgagagttg cggcgttgtc gcggtagacg tccgcgttgc cgggctcgat ctccaccatc
     2041 tcgcgagcaa tgacgttgcc aagggaggcc aggcgcgtgg ggtcgagcca gacatgtgga
     2101 tcggccgcac cggcctcgat cagctcgtcc tgagtgtcga gcgcatcgac cattcgcgtc
     2161 cccgataact cgccgatgac gtcctcgact gccggttgga acccctgacc gatgtagacg
     2221 atgagatcgg cctgagccag agagcgaacc tggtcgggag cgagctcgag atcgtggggc
     2281 tcgacccccg gtggcgacag gttcgtgact gtggcgttgc gtccgactat ctcggttgcc
     2341 acgaattcga ggggataaaa ggcggcgatc accttgggcc cctccgaggt tcctgactca
     2401 ccgccggccc ggccacagcc gccgacggct agcatgacca gggcaacaag ggccaaaacg
     2461 atgggtcgca tggggtcagg ttaggactta ttgaaaacga ttgtcaaatc cgttagccat
     2521 acagaggaac tcaggctctt acgtttggtc cggtccgctc tgccccgg
//
LOCUS       DATMED010000304         7541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_625266, whole genome shotgun sequence.
ACCESSION   DATMED010000304 DATMED010000000
VERSION     DATMED010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625266"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VNP73_07785"
     CDS             complement(<1..1085)
                     /locus_tag="VNP73_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWL65859.1"
                     /translation="MAGPGKGLEGVVAAETAISDIDGQKGILSYVGFDIHDLADHATF
                     EEVIYLLHHLELPSEQQLGDIRNQIASEREPNELAMDLLPHLAEHSAPMSMLRTAISV
                     SSAVDPDGWDTSPEANYRKAIRLCAVMPTLVAYYDRMRRGEPTIPSDGSLSQAANFLY
                     MLKGDKADDEAARIFDVCLILHADHTMNASTFAARVCAATLSDIHSSITAAIAALKGP
                     LHGGANEQVMRMIKEIKEFGGKEKARDYIIDRLRNKEKVMGFGHRVYKTEDPRATHLR
                     RMAQELAGRTGDPSNYEISEEIEKAVMDEKGIYPNVDFYAASVYGYLQIPTDLFTPVF
                     ACSRVSGWTAHVREQYADNRLIRPDHA"
     gene            1154..2251
                     /locus_tag="VNP73_07790"
     CDS             1154..2251
                     /locus_tag="VNP73_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HWL65860.1"
                     /translation="MSQTTHRVTFIPGDGTGPEIADATRRVLEATGVKFDWDVQHAGV
                     DVMEEYGTPLPDNVLDSIRDTKVAIKGPITTPVGTGFRSVNVALRKELDLYMCLRPCK
                     SYPGVRSRYENIDLVVVRENHEDLYAGIEFEMGSPEARKLIDFLADLGARRIRDDSGI
                     SIKPISVTGSQRIVRGAFDYAVKEGRKKVTAVHKANIMKFSDGLFLKTAQEVAEKEYA
                     DSGVEFEDRIVDNMCMQLVQKPELYDVLVLPNLYGDIVSDLGAGLVGGLGVAPGGNIG
                     DDAAVFEPTHGSAPKYAGQNKVNPMAMMLSGVLLLRHLDEGDAADRLEGAIASVIAEG
                     KTVTYDMKPNRDDPSAVGTSEVADGIIEKMG"
     gene            2254..3189
                     /gene="mdh"
                     /locus_tag="VNP73_07795"
     CDS             2254..3189
                     /gene="mdh"
                     /locus_tag="VNP73_07795"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWL65861.1"
                     /translation="MSKVTVVGAGNVGSNAARRVAEKNLADEVWMIDIVEGLPQGLAL
                     DINHSASVVGFDTLVQGTNDYKDTAGSDVVVITAGLPRKPGMSRMDLLDTNAKIVTEV
                     TENIVANSPDAVLIVVSNPLDEMTYLAAVVSGFPRERVMGMAGVLDSARLRFFIAEKL
                     NVSPQDVEAITLGSHGDQMVALPRHATVQGKPLPELADEKTLEELFQRTRDAGAEIVD
                     YLKKGSAFYAPGASSAAMTNAILGDTGDVMPVCAWTTGQYGISDVYLGVPAKLGTGGV
                     QEIVELDVNDDELGKLKEAAEAIRTKCKELDEVRS"
     gene            complement(3186..3989)
                     /locus_tag="VNP73_07800"
     CDS             complement(3186..3989)
                     /locus_tag="VNP73_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65862.1"
                     /translation="MMKVLSVVGAVLLLAALAAPAEGSHPTQICLDVLPERAYPTAGD
                     DIVDDLGAHVGATDDDHEEPHQEGCVTGQGSGSGSIVDFEIDGKPDPDESFSPESPDM
                     TCAIPEGDSSCFVFPPGSDGGEQIIHAWIDFDADDSTVELDVEEAVEDEDTDATDVVL
                     WTWTQDCCGDYRYDTTVTIRYSRDEFAGLVRSGYRRCEKRWVIVRRERRGEDQIVGRA
                     QTDDLAEWNITRKAAHGRYYANVRRKVFTTQEGYWVVCTTDRSPVIQVR"
     gene            complement(3986..4828)
                     /locus_tag="VNP73_07805"
     CDS             complement(3986..4828)
                     /locus_tag="VNP73_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65863.1"
                     /translation="MSEMKIVVLVAAVLVMGTVAIPAEGSHPTRQCIDAEPDFHYGVV
                     NDGKTDRLRVSLGVSDDENHPPKEGCITEDGTGSGQETEIDFEITGESDPDDSDTPES
                     PDRTCTIPRDDDTCRITPPSSAGGFQVTRAWVDADNSNATVEADLNEGQDESEDPGEV
                     EPDFTDVVYWGWTHRDEGPTGRSFDVRVTIRFDDNEFHGRVFSDKDRCFRYRIVTLRQ
                     LRDGEAVVVARVKANERGRWFIGGGSGPRGRFYVRVRQHEWTTRSDTHAVCENDRSPT
                     IRVP"
     gene            complement(4821..6293)
                     /locus_tag="VNP73_07810"
     CDS             complement(4821..6293)
                     /locus_tag="VNP73_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL65864.1"
                     /translation="MSGLGSRARKSPEAVAVVEHPRSITYGDFDKRQRELGGLFKKAR
                     FKSGDRIAVLSANRTEVLEVVVAALRRGIIPVPINPLLTPSEVSYVLEDSAARWMFTD
                     RTLEPHPQLERLITFGDAYERSLTEAKPAPLAENVRGRPMHYTSGTTGMPKGVWVEPY
                     DAPRAAEVSRAFRELWEIASEDIHLICSPLAHSAPLRFSIRTLEAGGTVILQKKFEAE
                     ETMAAIALFGVTSTFMVPTHLERILALGRRTLMRYDTSSVRLLAHAGAPIRTETKHEV
                     LALFPSGSVWEFYGSTEGQATRISTEEWIEKEGSVGRALPGAEVLIVDDAGTEKRAGE
                     VGEVWVRDPNGDEFEYWGDPGKTERAWRDDAFSVGDLGYLDRDGYLFLTGRKHDTIIT
                     GGVNVYPQEVEKVLCEHPAVAEALVYGAEHDEWGQEVRAAVVPNGDMPLQPELLKEWA
                     RERLAGFKLPRTIEVVESLPRTATGKLKRQPPGDPERLDE"
     gene            complement(6295..6849)
                     /locus_tag="VNP73_07815"
     CDS             complement(6295..6849)
                     /locus_tag="VNP73_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65865.1"
                     /translation="MPELLRRRFPPFVTMAVVVALITIAVFRLPDSLIEQLENSELLS
                     LSARGWAFRLLCLFSVAQAVYVGLIVFRIDRVKKAREDDPKVKSMSKPRFMTSLARNA
                     ATISLLTIAYGLAAVGFTGFRAGFWFFFALALLQGVWYFRELGQIARWLDFQPETSIQ
                     ASGGTWEPGPGYCPPLARGLSPQP"
     gene            6940..7491
                     /locus_tag="VNP73_07820"
     CDS             6940..7491
                     /locus_tag="VNP73_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWL65866.1"
                     /translation="MDMVADAALAERLLVRDETALRSVIDLYGDVVFGMARRIVIEPS
                     LAEEVAQDTFLALWRRPGAFDPGRGSLKGFLAGIARNKAIDLVRREESLRRTKDSLLA
                     EAEQASEPPSEHERIEEGQEVAAALRGLSLVQREAIVLAYYGGRTYREVAEELQIPEG
                     TAKTRLRDGLIRLKLLLSSEGSD"
BASE COUNT         1407 a         2325 c         2366 g         1443 t
ORIGIN      
        1 tacgcgtgat cgggacgaat caggcgattg tcggcatatt gctcgcgcac gtgggccgtc
       61 caccccgaca cccggctgca cgcgaagacg ggcgtgaaca ggtccgttgg gatctgcagg
      121 tagccgtaga ccgaggccgc atagaaatcg acgttgggat agatcccctt ctcgtccatt
      181 acggccttct cgatctcttc cgagatctcg tagttggacg ggtcgccggt ccttccggcg
      241 agctcctggg ccatgcggcg aagatgggta gcccgcgggt cttcggtctt gtagaccctg
      301 tgtccgaaac ccatcacctt ctctttgttg cgcaggcgat cgatgatgta gtcccgggcc
      361 ttctccttgc cgccgaactc cttgatctct ttgatcatgc gcatcacctg ctcgttggcg
      421 ccgccgtgga gggggccctt cagggcggcg atcgcagcgg tgatcgaaga atgaatgtcc
      481 gagagtgtcg cagcacaaac tcgagcagcg aatgtcgaag cgttcatcgt gtggtctgcg
      541 tggaggatca agcagacgtc aaagatccga gcggcctcgt cgtcggcctt gtcacccttg
      601 agcatgtaga ggaagtttgc cgcctgcgat agggagccgt ccgaagggat cgtcggttcg
      661 ccccgacgca tccggtcgta gtaggcgacc aaggtgggca tcacagcgca caggcggatc
      721 gccttgcgat agttcgcctc ggggctcgtg tcccatccgt caggatcgac cgcggaggac
      781 accgagatcg ccgtgcgaag catcgacatg ggggccgagt gctcggccag gtgtggcagg
      841 aggtccatgg cgagctcgtt gggctcgcgc tcgctcgcta tctggttacg gatgtcacca
      901 agctgctgtt cgctcgggag ctcgaggtga tgaagtaggt agatgacttc ttcgaacgta
      961 gcgtggtccg cgagatcgtg gatgtcgaag ccaacgtaag aaaggatgcc cttctggccg
     1021 tcgatgtcgg agatcgcagt ctcggcggcg acgacaccct ctaaaccctt gccaggccct
     1081 gccatgggac caaggctatc aggtaggttt cctcgaacta cctggacaaa cgcctacaac
     1141 aggagtgcct cgcatgtcac aaaccacgca tcgagtgacc ttcattcccg gcgacggaac
     1201 gggccccgag atcgccgacg cgacccggag ggttctggaa gccaccgggg tcaagttcga
     1261 ctgggacgtt cagcacgcgg gtgtggacgt tatggaggag tacgggacgc cgcttcccga
     1321 taacgtcttg gattcgatcc gcgacacgaa ggttgcgatc aagggtccca tcacgacccc
     1381 agtgggaacc ggtttccgca gcgtgaacgt tgcgctaagg aaggaactgg acctctacat
     1441 gtgcctgagg ccgtgcaagt cgtatcccgg cgtcaggtcc cgctacgaga acatcgatct
     1501 cgtcgtcgtg cgtgagaacc acgaggatct ctacgccggg atcgagttcg agatggggtc
     1561 gccagaggca cgcaagctga tcgatttcct ggcggatctg ggggcacgtc gcatccgcga
     1621 cgactcgggt atctcgatca agccgatctc cgtgaccggc tcccagcgca tcgtgcgcgg
     1681 tgccttcgac tacgcggtta aggaagggcg caagaaggtc accgccgtcc acaaggccaa
     1741 catcatgaag ttctccgatg gtctattcct gaagactgca caagaggtgg ccgagaagga
     1801 gtacgcggac tccggcgtcg aattcgaaga ccggatcgtg gacaacatgt gcatgcagct
     1861 ggtccagaag ccggagctgt acgacgtgct cgtgcttccg aacctctacg gtgacatcgt
     1921 ctcggatctc ggggcgggtc tcgtcggggg actgggggtc gcgcccggcg gaaacatcgg
     1981 cgacgacgcg gctgtgttcg aaccgacgca cggttctgct ccgaagtatg caggccagaa
     2041 caaagtcaac ccgatggcga tgatgttgtc cggcgttctg ctgctgaggc atctggacga
     2101 gggtgacgcg gcggaccgtc tcgaaggcgc gatcgcttca gtcatcgcag aaggcaagac
     2161 cgtgacctac gacatgaaac cgaaccgcga cgacccgtct gcggtcggaa cttccgaggt
     2221 cgccgacggg atcatcgaga agatgggcta gccatgagca aggtcacagt tgtaggtgcg
     2281 gggaatgtcg gttccaacgc ggcacgacgg gttgccgaga agaatcttgc cgacgaagtc
     2341 tggatgatcg atatcgtcga aggattgccg cagggtcttg cgctcgacat caaccattcg
     2401 gcctcggtgg ttgggttcga caccttggtg caggggacca acgactacaa ggacacggcc
     2461 ggctccgacg tcgtcgtgat cacggctggg cttcctcgca agccggggat gtcccggatg
     2521 gatcttctcg atacgaacgc caagatcgtc accgaggtga ccgagaacat cgtggcgaac
     2581 tcgcccgatg ccgtcctcat cgtcgtgtcg aacccgctgg atgagatgac gtatctcgca
     2641 gcggtcgttt ctggttttcc acgtgagcgc gtgatgggta tggccggcgt cctcgactcg
     2701 gcccgcttgc ggttcttcat cgccgagaaa ctcaacgtgt cgccgcagga cgtcgaggcg
     2761 atcacgctcg gctcgcacgg tgaccagatg gtggcgctgc cgcggcacgc gaccgtgcag
     2821 gggaagccat tgccggaact tgccgacgag aagacgctcg aggaactgtt ccagcgaacg
     2881 cgggatgcgg gggccgagat cgtcgactac ctgaaaaagg ggagcgcctt ctacgcgccg
     2941 ggagcgtcat cggccgcgat gacgaacgcg atcctgggcg acacgggtga tgtcatgccg
     3001 gtgtgcgcgt ggacgacggg ccagtacggg atctcggatg tttacctcgg cgtgccggcg
     3061 aagctgggta ccggcggcgt gcaagagata gtcgagctgg acgtcaacga tgacgaactt
     3121 gggaaactga aggaagcggc cgaagccatc cgcacgaagt gcaaggaact agacgaagtc
     3181 cgctcctaac gcacctggat cactggtgat ctgtcggtcg tacacaccac ccagtacccc
     3241 tcctgggtcg tgaagacctt tctgcggaca ttggcgtaat agcgcccgtg cgcggctttc
     3301 ctagttatgt tccactcggc caagtcgtcg gtttgggctc gacccacgat ctgatcctct
     3361 ccccgccgct ctcgcctaac gatcacccat cttttctcgc agcggcggta gccggaccgg
     3421 accagcccgg caaactcgtc ccgtgagtat cggatcgtga ccgtcgtgtc gtacctatag
     3481 tcaccgcagc aatcctgggt ccaggtccag aggactacgt cggtggcatc tgtgtcttcg
     3541 tcctccaccg cctcctcgac atccagctcg acagtcgagt cgtcggcatc gaagtcgatc
     3601 catgcgtgga tgatctgctc gccgccgtcg gagcccggcg ggaacacgaa gcaagagctg
     3661 tcgccctcgg gtatcgcgca cgtcatgtcg ggcgactccg gtgagaaaga ctcgtccggg
     3721 tcgggcttcc cgtcgatttc gaaatcgacg atcgatccgc tacccgaacc ctgtcccgtc
     3781 acgcaaccct cctgatgtgg ttcttcgtgg tcgtcatcgg ttgcgccgac atgggcaccg
     3841 aggtcgtcga cgatgtcgtc cccggccgtg ggataagcgc gttcgggcaa cacatcaaga
     3901 cagatctgag ttgggtggct accttcggcg ggcgcggcaa gtgctgccag cagcagcacc
     3961 gcccccacga cgctgaggac tttcatcacg gcacgcggat cgtgggggat cggtcgttct
     4021 cgcagaccgc gtgggtatcc gaccgagtgg tccactcgtg ctgtcgtacg cgcacataga
     4081 accttcctcg ggggcctgat ccaccgccga taaaccatcg cccgcgctcg ttggctttga
     4141 cccgtgcgac cacgaccgct tcgccgtcgc gcaattgcct caaggtgacg atgcggtaac
     4201 ggaagcagcg gtccttgtca ctgaagacgc gcccatggaa ctcgttgtcg tcgaaccgga
     4261 tcgtgactct gacatcgaag gatctgccgg tcgggccctc gtcgcggtga gtccagcccc
     4321 agtagacgac gtcggtgaag tcgggttcga cttcaccggg atcctccgac tcgtcctggc
     4381 cctcgtttag atcggcttcc actgtggcat tgctgttgtc ggcgtcgacc cacgctcttg
     4441 tgacctggaa acctccggcg gaagaggggg gagtgatcct gcaggtgtcg tcgtcgcggg
     4501 ggatcgtgca cgttcggtcc ggtgactcag gtgtatcgga atcatcggga tcgctctcac
     4561 ccgtgatctc gaagtcgatc tcggtctctt gaccagaccc cgttccatcc tcagtgatgc
     4621 aaccttcctt cggcggatgg ttctcgtcgt cggaaacacc gagtgacact cggagacggt
     4681 cggtctttcc gtcgttgacg accccgtaat ggaagtcggg ttcggcgtcg atgcattgtc
     4741 gggtcgggtg gctgccttcg gccgggatcg cgacggtccc catcaccaac acggctgcca
     4801 cgagcacaac gattttcatc tcactcatct aaacgctcgg ggtcgccggg gggttgcctc
     4861 ttcagcttgc ccgtcgctgt ccggggcaga gattcgacca cttcgatcgt tcgaggcagc
     4921 ttgaatcccg ccaggcgctc acgagcccat tccttcagca actcaggctg cagcggcatg
     4981 tcaccgttcg gaaccacggc cgcccgcacc tcctgccccc attcgtcgtg ctcggcccca
     5041 tagaccagcg cttccgcaac ggccgggtgc tcgcacagca ccttttcgac ctcctgaggg
     5101 tagacgttga cgcccccggt gatgatcgtg tcgtgcttac gccccgttag gaagaggtat
     5161 ccgtcccggt ccaggtagcc caggtctccg accgagaagg catcgtcccg ccacgctcgc
     5221 tcggtctttc ctgggtcgcc ccagtattcg aactcgtctc cgttcgggtc ccgaacccac
     5281 acctcgccga cctcaccggc ccgcttctcc gtacccgcgt cgtcgacgat gagcacctca
     5341 gctccgggaa gtgcgcgccc gacgctccct tccttctcga tccattcctc cgtcgagatc
     5401 ctcgtcgcct ggccctcggt cgatccgtag aactcccaca cggacccact cgggaagagg
     5461 gccagcacct cgtgtttggt ctcggtccgg atcggagcac ctgcatgcgc tagaagacgg
     5521 accgacgaag tgtcgtatcg catcaacgtc ctgcggccca gagccaggat ccgctcgagg
     5581 tgggtcggca ccatgaacgt cgacgtcacc ccgaagagcg cgatcgccgc catcgtttcc
     5641 tctgcctcga acttcttttg gaggatgacc gtgccgcccg cctccaacgt tcggatcgag
     5701 aaccgtagtg gagcggagtg cgcaagtggc gaacagatca gatggatgtc ttcggaagcg
     5761 atctcccaca actcgcggaa ggcccgcgac acctcggcgg ctctcggtgc gtcgtaaggc
     5821 tcgacccaca cacccttcgg catcccagtc gtacccgacg tgtagtgcat cggtcggccg
     5881 cgcacgttct cggccagcgg agccggtttc gcctccgtca gcgaccgctc gtaggcatcg
     5941 ccgaacgtga tcagccgttc gagttgcgga tgcggttcga gtgtcctgtc tgtgaacatc
     6001 cacctagcgg ccgaatcctc gagaacgtag gacacttccg acggtgtcag aagaggattg
     6061 atcggaacgg ggatgatccc tcggcggagc gcggcgacga ccacttcgag aacctcggtt
     6121 cggttcgccg acaggacggc tatccgatcg cccgatttga agcgtgcttt tttgaacagt
     6181 cctccgagtt cccgctggcg cttgtcgaag tctccgtagg tgatgcttcg agggtgctcg
     6241 acaaccgcta ccgcctcggg tgacttacgg gctctgctcc ctaagcccga cacgtcaggg
     6301 ttggggcgag agaccgcgcg ccaggggtgg gcaatagccg ggtcccggct cccatgtccc
     6361 accgctcgct tggatcgagg tctccggctg gaagtcgagc cacctcgcga tctggccgag
     6421 ttcgcggaag taccaaacgc cttgcagtaa cgcgagcgcg aagaagaacc agaacccggc
     6481 ccggaaaccg gtgaagccga cggccgccaa tccgtacgcg atcgtcagca ggctgatcgt
     6541 cgccgcattg cgcgccagcg aggtcatgaa ccgcggcttg ctcatgcttt tgactttggg
     6601 atcgtcctcg cgagctttct tgacccggtc gatacggaag acgatgaggc cgacgtaaac
     6661 ggcttgagca acggagaaca gacacagcag gcggaaggcc catcctcgtg ccgacaaact
     6721 gagcagctcc gagttctcga gctgttcgat gagggaatcg ggcagtcgaa agaccgcgat
     6781 cgtgatcaaa gcgacgacga cagccatcgt gacgaatgga gggaacctgc ggcgaaggag
     6841 ttccggcacg gcccgatgct agtgccacag ggggcccggc atccggcgcc tgccccacat
     6901 ccgaaccacg aggagatgat tggctacgct ccacccttca tggacatggt tgcggatgcc
     6961 gccttagcgg aacgcctgct ggttcgcgac gagaccgccc tccgctcggt catcgacctt
     7021 tatggagatg ttgtgttcgg catggcccga cggatcgtga tcgagccgtc tttggccgag
     7081 gaggtagctc aggacacctt ccttgccctg tggcgtcgtc ctggggcgtt cgatccgggc
     7141 cgcggaagcc tcaagggctt cctcgcgggg atcgcccgca ataaggcgat cgacctcgtc
     7201 cggcgggagg aatcgctgag gagaaccaaa gattctctac ttgcggaggc agaacaggca
     7261 tccgaaccgc cctccgagca cgaacgcata gaggaagggc aagaggtggc ggcggcgcta
     7321 cgtgggctct cgctcgtcca gcgggaggcg atcgtcctcg cttactacgg aggtcgcacc
     7381 taccgcgagg tagccgaaga gctgcagata cctgagggca cagccaagac acggctaaga
     7441 gacggcctta tcaggctcaa gctgctcttg tcgtcggagg gatccgactg atggacagag
     7501 ggcatgagga gatgaaagaa cttctggccg cttacgcgct c
//
LOCUS       DATMED010000305         3503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2183473, whole genome shotgun sequence.
ACCESSION   DATMED010000305 DATMED010000000
VERSION     DATMED010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183473"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNP73_07825"
     CDS             <1..210
                     /locus_tag="VNP73_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006290673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWL65867.1"
                     /translation="MEVAENRDELFKLFRSELDKDRTRTQVFDISPLGLVQMTRKNVS
                     AGIVESFSDACPTCEGRGILIHDVD"
     gene            506..880
                     /gene="rplU"
                     /locus_tag="VNP73_07830"
     CDS             506..880
                     /gene="rplU"
                     /locus_tag="VNP73_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWL65868.1"
                     /translation="MYAVIRAGGKQHKVAPGDVLEIERVKDASDTLEFTPLLVVDDKG
                     KARTSKSELAKARVMAKVVGESAGDKVDVYKYRSKTGYRRRAGHRQKFTTIQIADIKL
                     TAGKAAAKTEKTEAKEDAEDGS"
     gene            870..1121
                     /gene="rpmA"
                     /locus_tag="VNP73_07835"
     CDS             870..1121
                     /gene="rpmA"
                     /locus_tag="VNP73_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWL65869.1"
                     /translation="MAHKKGAASSRNGRESHSKRLGVKIYGGQVVKPGMIIIRQRGTK
                     VHPGLNVKKGGDDTLFATADGTVSFHNLRGRRVVSVVAE"
     gene            1178..2443
                     /gene="obgE"
                     /locus_tag="VNP73_07840"
     CDS             1178..2443
                     /gene="obgE"
                     /locus_tag="VNP73_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWL65870.1"
                     /translation="MAFVDEARIHVGAGKGGDGSASFHHEPFKPKGGPDGGDGGEGGS
                     IILKADRSVGTLLELRDHPHVKAAPGEPGRKSRRHGARGRDRVVLVPLGTVVNTDDGT
                     LVADLALAGDEYVAARGGRGGRGNVRFATSTRRAPSFSEKGEPGEERSLRLELRLLAD
                     VGLVGWPNAGKSTLISRVSSAKPKIADYPFTTLVPNLGVVKAGDESFVMADIPGLIPG
                     AHEGKGLGDRFLRHISRAAVLVYIVDLAAMDRDPAGDVDALKLELGNFDPGLLERPSI
                     VVLNKVDAFPDDGAELLKAMPGALAISAVSGEGVDRLLTRLAARVHEARAATGGERAS
                     FVRYISRPEAVKVAREGAAWRVSGSEPERVVAITDLDNDEAVARLQKRLLRLGVERAL
                     EAAGARAGDEVRIGDAQFDFEPESGAANG"
     gene            2436..>3503
                     /gene="proB"
                     /locus_tag="VNP73_07845"
     CDS             2436..>3503
                     /gene="proB"
                     /locus_tag="VNP73_07845"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWL65871.1"
                     /translation="MVRPRLDPADRVVVKVGTTSLVDSSGVIDEARVTDICDQIAELR
                     GTGVDVIMVSSGAIAAGLEPLGLTKRPSDIPSLQAAAAVGQGRLLGRYARRFKELGWV
                     TAQMLLTQHDFMHRQQYLNARHTLDRLLSLHAIPVVNENDTVATDEIRFGDNDRLAAL
                     VGNLARARLLVLLTDAQGVHARDPRKAPDAPLLDEVERITPELERAAGGRGSELATGG
                     MASKLSAAWVATFSGVGVVVASAVEPDVLKRVAAGDKVGTYFHPQPHRASARRLWIAF
                     AQPPKGTIVVDDGAKRAVIDDKRSLLPVGVVDVKGEFSSGDIVDVAGANGGPFARGLV
                     RYDSKELEAARGRSTAELAGRE"
BASE COUNT          639 a         1048 c         1199 g          617 t
ORIGIN      
        1 atggaggtgg ctgagaaccg ggacgaactc ttcaagctgt tccgcagcga gctggacaag
       61 gaccgaaccc ggacccaggt gttcgacatc tccccgctcg gcttggttca gatgacccgc
      121 aagaacgtct ctgccgggat cgtggagtca ttctcggatg cttgccctac ttgtgaaggt
      181 cgcggcattt tgatccacga cgttgactag cccccaagcg aaaaacctcg cgaccggctc
      241 caacggaccg taactctggc gtatttcccc ccgttggagg accaagagca tcgtgctggg
      301 aagggagccg catgcctcag atatcggcgc tactgggggc gctggtcatg gttgtagtgg
      361 tacttctcgc cgccttcagc ttcatcgtgc tcggcggcat gccctagtcg accggtagcg
      421 gggcccggcg ggggccccga tcgccgactg atatcctttc tccttcgatc caacgaggac
      481 ctcctcgact tccaagggag tgaacgtgta cgcagtcatc cgcgcgggcg gcaagcaaca
      541 caaggtggcg cccggtgacg tgcttgagat cgagcgcgtc aaagacgctt cagacacgct
      601 cgagttcacg cccctgctcg tcgtcgacga caagggcaag gctcgcacca gtaagagcga
      661 gctggcgaag gctcgggtca tggcgaaggt cgtgggggag tcggcgggcg acaaggtcga
      721 cgtctacaag taccgcagca agaccggtta ccgcaggcgt gccggccaca gacagaaatt
      781 cactacgatc cagatcgcgg atatcaagct gacggcgggc aaagccgccg ccaagacgga
      841 gaagacagag gccaaggagg acgctgaaga tggctcataa gaaaggggcg gcctcctccc
      901 gcaacggccg cgagtctcac tccaagcggc tcggcgtgaa gatctacggg ggccaggtcg
      961 tgaagccggg gatgatcatc atccgtcagc gcggcacgaa ggtgcacccg ggcctcaacg
     1021 tcaagaaggg cggagacgac actctgttcg cgaccgctga tgggaccgtc tccttccaca
     1081 acttgcgcgg ccgacgtgtg gtgtcggtcg tggccgagta gcacaccttc ttttcgcggc
     1141 gcccctcgcg ccgcttcccc gggcctaggc tcctacgatg gctttcgtcg atgaagcgcg
     1201 catccacgtg ggtgccggca agggtgggga tggctcggcc tcgttccatc acgagccgtt
     1261 caagcccaaa ggtggccccg acggaggcga cggtggtgag ggcggaagca tcatcctcaa
     1321 agccgaccgg tccgtaggga cgctgctcga actgagggat caccctcatg tgaaggcggc
     1381 gccgggcgag cccgggcgca agagccggag acatggagcg cgtgggcgcg atcgggtcgt
     1441 tctcgttcca ctgggcaccg tggttaacac cgacgacggg acgctcgttg cagacctcgc
     1501 actcgccggc gatgagtacg tggccgcacg gggtggccga ggtggtcggg gcaacgtccg
     1561 cttcgcaaca tccacacgca gggcaccttc cttctccgag aaaggcgaac cgggggagga
     1621 gcgctcgctg cgtttggagt tgcgactcct ggccgacgtc ggcctcgtcg gatggcccaa
     1681 tgccggtaag tcaacgctca tatccagagt gtcttcggct aagcccaaga tcgcggacta
     1741 cccattcacg accctcgtcc ccaacctcgg tgtagttaag gccggcgacg agtccttcgt
     1801 catggccgac atcccgggac tcatcccggg tgcgcacgag ggcaagggat tgggagatcg
     1861 attcttgcgg cacatcagcc gggcggcggt cctcgtgtac atcgtcgatc tggccgcgat
     1921 ggatagagat cccgccgggg acgtagatgc gttgaaactg gagttgggga acttcgatcc
     1981 aggtctactc gagcgtccct cgatcgtcgt tctcaacaag gtcgatgcat tcccggacga
     2041 cggcgctgag ctcttgaagg cgatgcccgg cgcgctggcg atctcggccg tcagcggtga
     2101 aggggtcgat cgtttattga cgcggctcgc agcgcgggtg cacgaagcga gagccgctac
     2161 cgggggggag cgtgcctcct ttgtgcgcta catctctcgt ccagaggctg tgaaggtcgc
     2221 gcgggaagga gcggcgtggc gcgtgtctgg ttccgagccg gaacgcgttg ttgcgataac
     2281 ggatctcgat aacgacgagg ccgtcgcccg tcttcagaag cggcttctga ggctgggggt
     2341 cgaacgagcg ctcgaggccg caggagcccg agcgggtgac gaggttcgca tcggcgatgc
     2401 tcagttcgat ttcgaacccg agagtggagc tgcgaatggt tagaccccgg ttggatccgg
     2461 ccgatcgtgt cgtcgtcaag gtcgggacga cctctctggt ggactcgtcc ggtgtcatcg
     2521 atgaagctcg cgtcaccgac atctgcgatc agatcgccga gctgcgtggg acgggcgtgg
     2581 acgtgatcat ggtgtcttcg ggcgccatcg ccgccgggct tgagccgctc gggctgacga
     2641 agaggccctc ggacatcccc tcgttgcagg cggctgcggc ggtgggccag gggcgcctgc
     2701 tgggtcggta cgcgaggcga ttcaaggagc tggggtgggt aaccgcccag atgttgctca
     2761 cgcaacatga cttcatgcac cggcagcaat atctgaacgc tcgtcacact ctggacaggc
     2821 ttctatctct gcatgccatt ccggttgtga acgagaacga caccgtcgcg accgacgaga
     2881 tcaggttcgg ggataacgat cggctggccg ctctcgtcgg gaacctggcg cgggctcggt
     2941 tgctcgtgct gttgaccgat gcgcagggag tgcatgcacg ggatccacgc aaagccccag
     3001 atgctccgtt gctcgacgag gtcgaacgga taactccgga gctcgaaaga gccgcggggg
     3061 gccggggctc cgagctggcg acaggaggca tggcgtcgaa gctcagtgct gcatgggtgg
     3121 caacattctc aggtgtcgga gtggtagtcg catcggccgt cgagccggac gttctcaagc
     3181 gcgtggcagc cggtgacaag gtgggcacgt acttccatcc gcaaccgcac cgagcgtcgg
     3241 ctcgccgctt gtggatcgca ttcgcccaac cccccaaggg cacgatcgtc gtggacgatg
     3301 gcgccaagag agccgtgatc gacgacaagc ggagccttct tccggtcgga gtggtcgacg
     3361 tcaagggcga gttctcctcg ggcgacatcg tcgatgtggc cggggcgaac gggggcccct
     3421 tcgcgcgcgg cttggtacgc tatgactcta aggagttaga ggccgcccga ggccgctcca
     3481 cggcggagct ggccgggcgt gaa
//
LOCUS       DATMED010000306         5555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1397356, whole genome shotgun sequence.
ACCESSION   DATMED010000306 DATMED010000000
VERSION     DATMED010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397356"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..629
                     /gene="mraZ"
                     /locus_tag="VNP73_07850"
     CDS             198..629
                     /gene="mraZ"
                     /locus_tag="VNP73_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWL65872.1"
                     /translation="MSFTGEFRHTIDAKGRLIVPSRMRDELAGGNVVLTRWLEECIAL
                     WSQQGWDEMEAKLREAGSSSRDARNFVRLVSSSAHIDEVDKQGRITVPQRLRDLAGIT
                     RDVVVIGALNRGEIWSPQRWEHQRDSVDEERLAELAEGLNF"
     gene            641..781
                     /locus_tag="VNP73_07855"
     CDS             641..781
                     /locus_tag="VNP73_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65873.1"
                     /translation="MSRYTIGVTANRARELTTGIALLALTGSSVGGFLGMVMIATRAL
                     GR"
     gene            786..1739
                     /gene="rsmH"
                     /locus_tag="VNP73_07860"
     CDS             786..1739
                     /gene="rsmH"
                     /locus_tag="VNP73_07860"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWL65874.1"
                     /translation="MDHEPVLADSVVELLLPALQGGGVFVDATVGRGGHSARLLEAAP
                     QAEVIALDRDGLALEESKSNLAAYAQRVRTHRANFSSLETVLERFGSPPVRGVLFDLG
                     VSSPQLDTAGRGFSYRSEGPLDMRMDPSQPLSAATVVNEYPEGDLARVIKQFGEERFS
                     ARVARAIVAARPIGSTTRLADVVKNAIPAATRRTGGHPARRTFQAIRIEVNDELRNLE
                     RGLAAAHDVLDPGGRIVALTYHSLEDRIVKNFFNEAADGCTCPPDFPVCACGASATLR
                     VLTRRPLRPGDAEMKSNPRASAAKLRAAEKLGDAVAGEDAP"
     gene            1736..2227
                     /locus_tag="VNP73_07865"
     CDS             1736..2227
                     /locus_tag="VNP73_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65875.1"
                     /translation="MSTRAASVKDDRKGFTGLHLLSREDRAKKRKGTRRLVTIGATVL
                     LVVTILVAILLEQVILAQSAFELSSLRESLTAEEARHEELLMEAAKRDNAARIERFAR
                     QSLGMMDPAPGHIHYVVADVRTGDALAEGAVAGRPIALKRAGVTHLGSVTASGGNYGG
                     LSP"
     gene            2410..4122
                     /locus_tag="VNP73_07870"
     CDS             2410..4122
                     /locus_tag="VNP73_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWL65876.1"
                     /translation="MFFVFSLAFLGMAVRLVSLQILDAPAYARLAADQREREVILPAR
                     RGAILDRTGEPLAISIDMFMVYTDHIHIDDPHETATLLAPLIDQEPVQVEAKLAPSFE
                     GDQFEFLARQMSPKIARRIKALDLPGIYMKEEPKRYYPGGRLASHVLGFADIDGKGLE
                     GIEKQYDSILQGRPGRVTLEQDPAGRALPQAEFSEKRPRPGRSLFLSLDKELQYFTEL
                     TLADATQRYHAEAGTAIVMRPNTGEILALANVPTYDANHPGDALPEARRNRVLTDVYE
                     PGSAFKIVTASAALERHVVTPRTTFSVPDTFRYSDRVFHDSHPHPAETMSTAEIIRES
                     SNVGTIQIGLRLGGKRLDRFVRKFGFGSRTGLDFPGESEGIVISRKDWTGPTIATIPI
                     GQGIAVTPLQLVNAYSALANGGVAVEPKLLHATLGSNGRVEPSSPPARHRVLSRKTAR
                     KMVKILESVVEKGTGVEAQIPGYEVAGKTGTAQKPLPGGGYGHSYVASFAGFAPASRP
                     AIVVLVTLDDPSPIWGGSTAAPTFRTIAEFTLRHLGVAPTENAERAVREIEADVAREP
                     AAHD"
     gene            4138..>5555
                     /locus_tag="VNP73_07875"
     CDS             4138..>5555
                     /locus_tag="VNP73_07875"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWL65877.1"
                     /translation="MGSQVRPLTLGAIASSLASLVVDRSGETSVTVLRPAYDSRAVEP
                     GDLFFCISGLVADGHAFADDAVAAGAVALCTTRRLDKGLPELVVADTRRALALVSAEM
                     MGRPSDDLLTIGITGTNGKTTTAFMIEAILAAAGRSPGLIGTIETHVGGERRPGVRTT
                     PESLDLQMLLREMVDKGADSVAMEVTSHALVLQRVEGIRFRVAVFTNLSQDHLDFHSN
                     MEDYFEAKRSLFTSERAQAGVVNVDDVYGRKLLENAAIPCIGYGMSEDAEVRASDVEL
                     DPSGTRFRLIAGGEDVLVATTLVGGFNVYNCLGAAAACLQAGIDLVAVARGVSGLNAV
                     PGRFEAVDSGQPFTVAVDYAHTPDSLDNVLRAAREMVARTGGKVLCVFGAGGDRDRAK
                     RPLMGAVAARGADVVIVTSDNPRSEEPASIVSQIVEGVIAESPEGPDEVLVDRRAAIE
                     TAIGMALAGDVVVIAGKGHETG"
BASE COUNT          994 a         1761 c         1818 g          982 t
ORIGIN      
        1 caccgttgcg tatgcccgaa ttacaagagt gtaattgggg cgttaaacgt tgacttcaag
       61 gctcgtaggg gtcaatctct cccactaagt ggagtgttgt ggaggaaagt ggggtaaccc
      121 accgaactcc aggcgccacc agtggcggcc tcggccgtcg cgcagaacag attcggtcgg
      181 caaggggagt cggttggttg tcattcaccg gggagttccg ccacacgatc gacgcgaaag
      241 ggcgtctgat cgttccgtcg cgcatgaggg acgagttggc cggcgggaac gtcgttctga
      301 cccgctggct cgaggagtgc atcgcgctgt ggtctcagca gggctgggac gagatggagg
      361 ccaagttgcg tgaggccggc agttcgtccc gggacgcccg caacttcgtc cgcctggtgt
      421 cgtccagcgc tcacatcgac gaggtcgaca agcaaggccg gatcacggtg ccgcagcggc
      481 tgcgcgatct ggccgggatc acccgtgacg tcgtggtcat cggggcattg aaccggggcg
      541 agatctggag cccgcagagg tgggagcacc agcgggacag cgttgacgaa gagcggctgg
      601 cagagctggc cgagggtctc aacttctaaa gggggaagag atgtcgcgct acacgatagg
      661 tgtcactgcg aaccgggccc gcgagctgac cacggggatc gcattgctgg ccctgactgg
      721 atcctcggtc gggggcttcc tcggcatggt catgatcgct acgcgggctc tcggacgata
      781 gagatgtgga tcacgagccc gttctcgcag actccgtcgt cgagttgctc ctgcctgcac
      841 tgcaaggagg aggcgtcttc gtcgacgcaa ccgtgggtcg aggtgggcac agtgcgcgcc
      901 tcctggaagc cgcgccgcaa gccgaagtca tcgcgctcga ccgggatggc ctcgccctgg
      961 aggaaagtaa gtccaacctc gccgcgtacg cacaacgcgt acggacccat cgtgcaaact
     1021 tctcgagcct cgagaccgta ctggaacgat tcgggtctcc gccggttcgc ggagtccttt
     1081 tcgaccttgg agtttcctcg ccccagctcg acacggcagg acgagggttc agttaccgga
     1141 gtgagggacc tctcgacatg aggatggatc cttcccagcc cctgtccgcg gccacggtcg
     1201 tgaacgagta tcccgaaggc gatcttgctc gcgtcatcaa gcagttcggc gaagagcgat
     1261 tctcggcccg cgtcgcgcgc gccatagtcg cggcacgacc cataggctcg accacgcggc
     1321 ttgcggacgt agtcaagaac gcgatcccag ccgctacccg ccgaaccggg ggtcaccccg
     1381 cgcgacgcac cttccaagcg atccggatcg aggtcaacga cgagctcagg aacctcgagc
     1441 ggggactggc cgcggctcac gatgtgctgg atcccggcgg ccgtatcgtc gcgttgacct
     1501 atcactcgct cgaagatcgg atcgtcaaga acttcttcaa cgaagccgcg gacggttgta
     1561 cctgccctcc ggacttcccc gtgtgtgcat gcggggccag cgcgacgctg cgggtactca
     1621 cgcgacgtcc cctccgtccc ggtgacgccg agatgaagtc gaatcctcgg gcttcggctg
     1681 cgaagctgag agccgcggag aaactcggtg acgccgtcgc cggcgaggac gccccatgag
     1741 cacccgggcc gcttcggtta aagatgatcg caagggtttc accggcctcc atctactgag
     1801 tcgggaggac cgtgccaaga agaggaaagg gacccgccga ctcgtcacga tcggggcgac
     1861 cgttctgctt gtggtcacga tcctggtcgc catcctgctg gagcaggtga tcctggccca
     1921 gtctgcattc gagctctcga gcctgcgcga gagccttacc gcagaagagg ctcgtcacga
     1981 agagctcctc atggaggccg cgaagcgcga caacgcggcg cgcatcgagc ggttcgcgcg
     2041 tcagtcgctc ggcatgatgg atcccgctcc cgggcacatc cattacgtcg tggccgatgt
     2101 ccgtacgggt gatgcgctgg ccgaaggagc ggtggcgggt cgcccgatcg ctctcaagcg
     2161 tgcgggcgtg actcatctgg gttcggttac cgcctccggc gggaactacg ggggattatc
     2221 accctgatgc caccgcgccg ttcgagacat gaaccgatca ggaggtcgac ccgtgggtcg
     2281 gcctcttttc tgatgccgcg atcgtcgaga cgatcgcggg gcccctctgc gtctggatcg
     2341 tctagacgat cgcgggggtc gcggcccccc cggcgtggcc tgacccgctc cgcacgcagg
     2401 ctcgtgttca tgttcttcgt gttctctctc gctttcctcg gtatggccgt gcggctcgtc
     2461 agcctgcaga tcctcgacgc tcccgcttac gcgcgcttgg ccgcagacca gcgcgaacga
     2521 gaggtcatcc ttcccgcacg acgaggtgcg atcctcgacc gcaccggcga gccgcttgcg
     2581 atctccatcg acatgttcat ggtctacacg gaccacatcc acatcgacga tccgcacgag
     2641 accgcgaccc tgctcgctcc actcatcgac caggagcccg ttcaggtcga agcgaaactg
     2701 gccccttctt tcgaaggcga ccagttcgaa ttcctcgcgc gccagatgtc accgaagatc
     2761 gcgcgtcgca taaaggctct cgatctgccc gggatctata tgaaggaaga gccgaagcgc
     2821 tattacccgg gcggccggct ggcctcacac gtgctcggtt tcgccgacat cgatgggaag
     2881 ggtctggagg ggatcgagaa gcagtacgac tcgatcctcc aggggcgacc cggccgcgta
     2941 accctcgagc aggaccccgc cgggcgcgct cttccacaag ccgagttctc cgagaagcgt
     3001 ccacgtcccg gccggtcgct ctttctcagc ctggacaaag agctccagta cttcaccgag
     3061 ctcacgctcg ccgacgcaac tcagaggtat cacgcggaag ccggcacggc catcgtgatg
     3121 agacccaata ccggcgagat ccttgcgctc gcgaacgtgc cgacctacga cgcgaaccac
     3181 cccggcgacg cactacccga ggcgcgcagg aatcgcgtct tgaccgacgt ctacgaacct
     3241 ggctcggcct tcaagatcgt caccgcgtcg gctgcgctcg agcgtcacgt cgtgactccg
     3301 cggacgacgt tctcggttcc ggacacgttc agatactcgg accgggtgtt ccacgactcg
     3361 catccgcatc cggcggaaac catgagtacg gccgagatca tccgagaatc gtccaacgtc
     3421 gggacgatcc agatcgggtt gcgtttagga ggcaaacgtc tcgacaggtt cgtccggaag
     3481 ttcggcttcg ggtcgaggac gggcctcgac tttcccggcg agtcggaagg gatcgtgatc
     3541 tcgcgcaagg actggaccgg tcccacgatc gcaacgatcc ccatcggtca ggggatcgcg
     3601 gtcactcctt tgcaattggt caacgcgtat tcagccctcg ccaacggtgg cgttgcagtc
     3661 gagccgaagc tgttgcacgc aacgcttggc tcgaacgggc gcgtcgagcc gtccagcccg
     3721 ccggcccgtc acagggtgct cagtcgaaag accgcacgga agatggtgaa gatcctcgag
     3781 agtgtcgtgg agaagggcac cggcgtcgag gcccagatcc ccggctacga agttgccggt
     3841 aaaacaggaa cggcacagaa gcctcttccc ggcggcggct acggtcactc ctacgtcgct
     3901 tcgtttgcgg ggttcgctcc cgcgtcgcgc ccggccatag tcgttctcgt cacactcgac
     3961 gatcccagtc ccatctgggg aggatccaca gcggccccga cgttcaggac gatcgcggaa
     4021 ttcactctgc ggcatctggg agtggcaccg accgagaacg ccgagagggc cgttagggag
     4081 atcgaggccg acgtcgctcg cgagccggcc gctcacgact agcatccgag atacgacatg
     4141 ggatcccaag ttcggccgct cactctgggc gcgatcgcga gttcgctcgc ttctctcgtc
     4201 gtggatcgaa gtggtgagac atccgtaacc gtccttcgtc ccgcgtacga ctcgcgggca
     4261 gttgagccgg gggacctctt cttctgcatc tcagggctcg tggccgacgg tcacgccttc
     4321 gcggacgacg ccgttgcagc aggggcggtc gccctctgca ccactcgtcg attggacaag
     4381 ggccttcccg agctcgtcgt ggcggacacc agacgggctc tagcgctcgt gtccgcggag
     4441 atgatgggcc gcccctcgga cgatctactc acgatcggga taacgggcac caatggaaag
     4501 acgacaaccg cgttcatgat cgaggcgatc cttgccgcgg ccggccggag ccctgggttg
     4561 atcggaacga tcgaaacgca cgtgggggga gagcgccggc cgggagtgag aacgacgccc
     4621 gagtccctgg atctccagat gctcctgcgc gagatggtgg acaagggagc ggattccgtc
     4681 gcgatggaag tcacgtccca tgccctcgtg ttgcagcggg tcgaagggat ccggttccgg
     4741 gtagcggtct tcacgaacct ctcccaggac cacctcgatt tccactcgaa tatggaggac
     4801 tacttcgagg caaagagatc gctgttcacg tccgagcgcg cgcaggccgg cgtggtgaac
     4861 gtcgacgacg tgtacggacg gaagctgctc gagaatgcgg cgatcccttg catcggatac
     4921 ggcatgagtg aggatgccga ggtccgtgca tcggacgtgg aactcgatcc ttccggaacc
     4981 cggttccgct tgatcgcggg gggcgaggat gtgctcgtcg ccaccaccct tgtgggtggg
     5041 ttcaacgttt acaactgcct aggcgcggcc gcagcctgtc ttcaggccgg gatcgatcta
     5101 gtagccgtcg cgcgaggtgt gagcggattg aacgcggtcc ccgggcgctt cgaggccgtc
     5161 gatagtggcc aacctttcac cgttgccgtg gattacgccc acaccccgga ttcgctcgac
     5221 aacgttctgc gggcggcgcg cgagatggtc gccaggacgg gagggaaggt gctctgcgtc
     5281 ttcggtgccg gtggcgaccg cgaccgtgcc aaacgaccgc tgatgggtgc ggttgcggca
     5341 cgtggggcag atgtcgtgat cgtgacgtcc gacaaccccc gcagtgagga gccggcgtcg
     5401 atcgtctctc agatcgtcga aggagtgatc gcggaatcgc ccgagggacc ggatgaggtt
     5461 ctcgtcgacc gccgcgcggc catcgagacc gcgatcggaa tggcgttggc cggtgatgtg
     5521 gtcgtcatcg cgggcaaggg ccacgagacc ggcca
//
LOCUS       DATMED010000307         4092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4467903, whole genome shotgun sequence.
ACCESSION   DATMED010000307 DATMED010000000
VERSION     DATMED010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467903"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VNP73_07880"
     CDS             <1..372
                     /locus_tag="VNP73_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWL65878.1"
                     /translation="MSTLGDPLADLGLMLVYWPQADDMSEFRDSFGAITATAAPGFPT
                     REEVIEQYARESGRDVAAVYYYWAFGFFKLAVILEGIHARFLQGKTLGEGFDSIGERV
                     PPLVMLGLWVLDQKAVSAIEA"
     gene            392..1609
                     /locus_tag="VNP73_07885"
     CDS             392..1609
                     /locus_tag="VNP73_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL65879.1"
                     /translation="MDFEHSKTSLEYQDKLNDFVEHHIYPNERAYAEQMMAQEDVHEQ
                     PPLMQQLQKEARSRGLWNMFLSKEMGGPGLSNIDYAPVCEIMGRSPIASEATNCAAPD
                     TGNMEILAEFGNEEQKKQWLEPLLEAEIRSCFGMTEPGIASSDPTNIATKIESDGDSY
                     VINGRKWWSSGAADPRCKVSLMIGRTNFEEPKDKASRYLQHSLVLVPMDAPGVKILRT
                     LPVFGFDAGHGHCEIVYENVRIPKSNLLGEEGGGFLIGQARLGPGRIHHCMRAIGMAE
                     RALELMCERAESRVAFGVKLSQQGIIRDWIAESRIRIEQARLLTMKAAWMMDTVGKEK
                     ARTEISAIKVAAADLATYVIDRAIQVHGGAGVSNDFPLAMMYAHARTLHIVDGADEVH
                     KMVIAREELKNYR"
     gene            complement(1606..3306)
                     /locus_tag="VNP73_07890"
     CDS             complement(1606..3306)
                     /locus_tag="VNP73_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWL65880.1"
                     /translation="MRFGLFYEHQLPRPWSEGAEGELLRNALDQIELADRLGIDYVWE
                     VEHHYLEEYSHSSAPEVFLAAASQRTKRIRLGHGIVQLPPGFNHPARIAERAATLDLV
                     SNGRVDLGTGEASSQMELGGFGVERDQKRAQWEEAIDVITRMFVEEPFAGHDGEFISV
                     PPRNVVPKPQQKPHPPLWVACSRRETILMAAERGIGALSFSFVEPEDAKEWVDDYYEL
                     LSSEKCVPAGFSVNPNLAVVVPLFCHQDEETAIERGIDGGHFFGYSLAHYYVFGSHRP
                     GRTNVWEEFQTHRDKYGFARQIITASGDALGVKLLEQGLGSMRGAVGTPKQIIELVER
                     YEAAGVDQVIFVAQCGNNVHEEVCESLELFGREVVPRFAEKADEVESEKLDRLSDVMD
                     KALARRAEARQSDPDYVVTPDGEPSQGRFLVLADREKKEASAQEQFLGQLRGKSESEL
                     DEMARGTPLVQMILSGMCMSFQPQATRGFTGDIQYLLQRSDKTEEWFVNIGEKEATVK
                     DGRAEAPALTISTDVPTFLRTASGEDNGALAMMQGRIKVQGNGEVLTRMAAMFGIDRF
                     "
     gene            3412..>4092
                     /locus_tag="VNP73_07895"
     CDS             3412..>4092
                     /locus_tag="VNP73_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65881.1"
                     /translation="MSGPVNVDRAALNRAGGLSLGETALAEAVEENGEQAEVADKTRR
                     LRLPWSRRDRAVEAVGETETPQDGAAEEAPEVSPLAYKTFSPKLPPALTAFGGLLAIG
                     GGLGAWVRATRVQTEGLAPEQVAVSMGYNDPEGLTIAVFGGVALITALLWLRRKPLFG
                     FLPAFAVKLVPVLASVAVIGLAIWQLPLIDQQAQALAADAIQDASFTAFHAGLGWGAW
                     LMIVASVLL"
BASE COUNT          797 a         1279 c         1256 g          760 t
ORIGIN      
        1 atgtcgactc tgggggatcc gctcgccgac ctcggattga tgctcgtgta ctggccccaa
       61 gccgacgaca tgtccgagtt ccgcgactcg ttcggggcga tcacagcaac tgcggcaccg
      121 ggcttcccaa cacgcgaaga ggtcatcgag cagtacgcgc gcgaatccgg cagggacgtc
      181 gcggccgttt actactactg ggcgttcggg ttcttcaagc tggcggtgat cctcgaaggc
      241 atccacgccc gcttcttgca aggaaagacg ttgggtgaag ggttcgactc gatcggcgag
      301 cgcgtgccgc cgctcgtgat gttggggttg tgggttctgg atcagaaagc ggtttcggct
      361 atcgaagcgt gatcaacata gggggcttgt agtggatttt gagcacagca agacatcgtt
      421 ggagtaccag gacaagctca acgacttcgt cgagcatcac atctacccga acgagcgcgc
      481 ctacgccgaa cagatgatgg cgcaagaaga cgtacacgag cagcctcctt tgatgcagca
      541 attgcagaag gaagctcgat cgcgcggctt gtggaacatg ttcttgtcca aggagatggg
      601 ggggccggga ctcagcaaca tcgactatgc cccggtctgc gagatcatgg gtcgcagccc
      661 gatcgcctcc gaagctacga actgtgcggc ccccgacacc ggcaacatgg agatcctcgc
      721 cgagttcggc aacgaagagc agaagaaaca gtggctcgag ccgttgctgg aagcggagat
      781 ccgctcttgc ttcggcatga ccgagcccgg catcgcgtcc tcggacccca cgaacatcgc
      841 aaccaagatc gagtccgacg gcgattccta cgtgatcaac ggacgcaaat ggtggtcgtc
      901 cggagcagcc gatccgcgct gcaaggtgag tctgatgatc ggacgcacga acttcgagga
      961 accgaaagac aaggcatcgc gctacctgca gcactccctc gtactcgttc cgatggatgc
     1021 gccgggcgtg aagatcttgc ggacccttcc ggtattcggt ttcgacgccg gtcacggtca
     1081 ctgcgaaatc gtctatgaga acgttcgaat cccgaagtcg aacctgctgg gtgaggaagg
     1141 cggcggattc ctgatcggtc aagcccgctt ggggcccggc cggatccacc actgcatgcg
     1201 agcgatcggg atggccgagc gtgccctcga actgatgtgc gaacgggcgg agtcccgcgt
     1261 tgccttcggc gtgaagctct cgcagcaagg gatcatccgc gactggatcg cagaatcccg
     1321 gatccgcatc gagcaggctc gtctcctaac gatgaaagcc gcttggatga tggacacggt
     1381 cggcaaggaa aaggcccgca ccgagatctc ggcgatcaag gttgcggcgg cagacctcgc
     1441 gacctatgtc atcgataggg cgatccaggt gcatgggggg gccggcgtca gcaacgactt
     1501 cccgctggcg atgatgtacg cccacgcgcg aacgcttcac atcgtggacg gagccgacga
     1561 ggtccacaag atggttatcg cccgcgagga gctgaagaac taccgctaga agcggtcgat
     1621 gccgaacatg gctgccatgc gggttagtac ctcgccgttg ccttgcacct tgattcggcc
     1681 ctgcatcatc gccaacgcgc cattgtcttc gccggaagcc gtgcgcagga acgtcggaac
     1741 atcggtcgag atcgtgaggg cgggggcctc ggctcgaccg tccttaacgg tcgcctcctt
     1801 ctctccgatg ttcacgaacc actcttcggt cttatcggag cgttgcaaca ggtattggat
     1861 gtcacccgtg aagccccgcg tcgcctgcgg ctggaacgac atacacatgc ccgacaggat
     1921 catctgaacg agtggagtac cgcgtgccat ctcgtcgagt tcggactcgc tcttgccgcg
     1981 tagttgaccc aggaactgtt cctgggccga cgcttccttc ttctctcggt cggcgaggac
     2041 caaaaagcgt ccttgtgacg gttcgccgtc cggagtgacg acataatccg gatccgattg
     2101 acgcgcctcg gcgcgccgag ccaacgcctt gtccatgaca tcggaaagac gatcaagctt
     2161 ctcgctctcg acctcatcgg ccttctcggc gaaccgtgga acaacctcac gtccgaacaa
     2221 ctcgagtgat tcacaaacct cttcgtgcac gttgttgccg cactgcgcga cgaagatgac
     2281 ctgatcgaca cctgcagcct catagcgctc gaccagctca atgatctgtt tcggagtacc
     2341 gaccgcaccg cgcatcgaac cgagtccttg ctcgagcaac ttgactccta gcgcgtcgcc
     2401 actcgcggtg atgatctgcc gagcgaaccc gtatttgtcc cgatgcgttt ggaactcttc
     2461 ccacacgttc gtccggccgg ggcggtgact accgaagacg tagtagtgcg cgagcgagta
     2521 accgaagaaa tgccctccat cgatccctcg ttcaatcgca gtctcctcgt cctggtggca
     2581 gaacagtgga acgacgaccg ctaggttcgg gttgaccgag aagcccgcgg ggacgcactt
     2641 ctccgacgac agcaactcgt agtaatcgtc tacccactcc ttagcgtcct cgggctcgac
     2701 gaacgagaac gacagcgccc cgatccccct ctctgctgcc atcaggatcg tctcgcgccg
     2761 actgcacgcg acccacagcg gagggtgcgg tttctgttgg ggcttaggaa cgacgttgcg
     2821 agggggcacc gagatgaact cgccgtcgtg accggcgaag ggttcctcta cgaacatccg
     2881 cgtgatgacg tcgatcgctt cttcccactg ggcccgcttc tggtcgcgct cgaccccgaa
     2941 tccgccgagc tccatctgcg acgaggcctc gcccgtaccg agatcgaccc gcccgttcga
     3001 aacgaggtcc agggtcgccg cgcgctcggc gatccgggcg ggatggttga agccgggcgg
     3061 tagctgcacg atcccgtgcc ccagacggat ccgtttggtc ctctgggacg ccgccgcaag
     3121 gaagacctcg ggggctgagg agtgtgagta ctcctcgaga tagtggtgct cgacctccca
     3181 cacgtagtcg atccccagcc ggtcggcgag ctcgatctga tccagagcgt tccgaagaag
     3241 ctcgccttcg gccccctcgg accacggcct cggcaactga tgttcgtaga acagcccgaa
     3301 tctcacggcg gccttagtac cacaaggcag gacaagcggc acgggacccc gaaatgattt
     3361 atgcacgagc gtcgtggtaa gaaatgcctg gtcgcacggc ataaatatga ggtgagcggg
     3421 ccggtcaacg tcgatcgcgc cgcgctgaat agagccgggg ggctttcatt gggcgagaca
     3481 gctcttgcag aggctgtcga ggaaaacggc gagcaggccg aggtcgcgga caagacccgc
     3541 cggctacgtc tcccttggtc gcgccgcgat cgggccgtcg aagcagtcgg cgaaaccgag
     3601 acgccgcaag acggggcggc cgaggaagcc cccgaggttt cgccgctggc ctacaagacc
     3661 ttctcgccga aacttccgcc ggcgcttacc gctttcgggg gcctcttggc catcggtggt
     3721 gggctgggtg cgtgggtgag ggccacccga gtccaaacgg aaggactcgc accggaacag
     3781 gtcgccgtga gcatgggcta caacgatccc gaaggcctca cgatcgcagt cttcggcggg
     3841 gtcgccctca tcaccgccct cctgtggttg cggagaaaac cactcttcgg gttccttccg
     3901 gcattcgcgg taaagctcgt cccggtcctc gcatcggtcg ccgtcatcgg actcgcgatc
     3961 tggcaactac ctctgatcga tcagcaggcg caggctcttg ctgccgacgc gatccaagat
     4021 gcgtccttca ccgccttcca cgcaggactc ggttggggcg catggctgat gatcgtggcc
     4081 tccgtccttc tt
//
LOCUS       DATMED010000308         2999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_252925, whole genome shotgun sequence.
ACCESSION   DATMED010000308 DATMED010000000
VERSION     DATMED010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252925"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..514)
                     /locus_tag="VNP73_07900"
     CDS             complement(29..514)
                     /locus_tag="VNP73_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWL65882.1"
                     /translation="MSRRAELLASRIEEGAASLVAFAEGLSDAEWATPTSTTDRRTVG
                     QVVNHVALVYPIEIDLARAIASGKAVTDVTWQVVAELNAGHAREHATVGKPEALELLQ
                     KNSGQAAETVRAFSDEELDRAAPFSLSFGAPVTAQFVVEDHALRHSWHHLARIRTALG
                     R"
     gene            complement(1052..1651)
                     /locus_tag="VNP73_07905"
     CDS             complement(1052..1651)
                     /locus_tag="VNP73_07905"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWL65883.1"
                     /translation="MPLRYADASRNLQDRFDTRRLADRLAEVKVHHEFTDADRQFIQV
                     QDMFFLATVDQQGQPTCSYKGGDPGFVTILDKATLAFPNYDGNGMYLSMGNVAQTARV
                     GLLFVDFERQRRMRVDGVAELAFEHDAMERYLECQFVVLVRATAIYPNCPRYVHQYKL
                     VERSPFVPRSGVATPTPEWKRSDWARDVLPTHDPATDRT"
     gene            complement(1661..2038)
                     /locus_tag="VNP73_07910"
     CDS             complement(1661..2038)
                     /locus_tag="VNP73_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65884.1"
                     /translation="MLFLFYAVAILLFPAGAVLAWRRGGERWLALLTVITVSALVGLA
                     LIVASERGGNRLVQTRGYTHTATRTLLFTGLALILPLVASAMSVWATGRRMHPGFVYP
                     IAVATALIGTAVGSIVAIYTLWS"
     gene            complement(2152..2592)
                     /locus_tag="VNP73_07915"
     CDS             complement(2152..2592)
                     /locus_tag="VNP73_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65885.1"
                     /translation="MNRTVKNTGRLTCESTRFVNVDLDIFSRVSLTGLVDAMGEAALV
                     LYVGGTGRKYEAHVELASSGHVGMTADRTIIGLTRLVKRLPPRYRKVWDSAKSREFNV
                     GIEAGLEPHSFEVRLDRRTVDAVREVGGALVVTVYAPDIAALRR"
     gene            complement(2685..>2999)
                     /locus_tag="VNP73_07920"
     CDS             complement(2685..>2999)
                     /locus_tag="VNP73_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65886.1"
                     /translation="TGRGEANLYGLCSFLIVESMRRGMRPKDAGIEALRRVKSATVER
                     RLLNSRGEPNFHLNFYVLNASGEHAGVALYAGPTAVYGVCTENGAENRPIEPLLQGSP
                     NA"
BASE COUNT          603 a          964 c          875 g          557 t
ORIGIN      
        1 aggcgcagtc agacagtcgg cgcaggagtc aacgacccaa cgcagtgcgg atccgcgcga
       61 ggtggtgcca gctgtgacgg agcgcgtggt cctcgacgac gaactgcgcg gtgaccgggg
      121 ccccgaagct cagcgagaac ggtgccgctc gatccagctc ctcgtcgctg aacgcgcgaa
      181 ccgtttcggc cgcctggcca ctgtttttct gcaagagctc gagtgcctcc ggcttcccga
      241 ccgtggcatg ctcgcgggcg tggccagcat tcaactcggc gacgacttgc caggtcacat
      301 cggtcaccgc cttcccactg gcgatggcgc gggccaggtc tatctcgatg gggtagacga
      361 gtgcgacatg gttcacgacc tggccgaccg tccggcggtc cgtcgtggac gttggagttg
      421 cccactcggc gtccgacagc ccttccgcaa aggccaccag gctggcagca ccttcttcga
      481 tgcgagaggc aagtaactca gcgcgacgac tcataccatt cctcctcagc gtgtggggcc
      541 gaatctattg agaggccctt gcctcgcgcg gcgccgccat cgcacgacgc ccgccagtcc
      601 cgacgccgcc agaacggcag cgcacagccc cagcggaatc gcgacaaagg cgagcccgat
      661 catatccccg ccgcgcgccc ctggggctac tacctatcaa atacttggag caacggacag
      721 acgtctcgga tttgaatagc ctctcaccct ccaacagacg gcagaacgat cgcggctcac
      781 ccgcggcgcc tcttgtccga tccaaaggtg aactgcgcga gcgccgattc ctcagacaga
      841 tttcgggcag ctgcgcggac actcgatgga acccaatcgg gacagggaat ctcttcggtc
      901 atgtgacgtc ccgacttggt gtggacctcc cgcacttgtg aaaggtgttc cgatggatgc
      961 cggtccgccc tttcgcggtt tcgagcacga caccgtgctt gcgaatgaat gcgagggcct
     1021 ggcgtaaggt catcgccatg ttgatgatct gtcaggtgcg atccgtcgcc ggatcgtgcg
     1081 tcggcagaac atcacgtgcc cagtccgagc gcttccactc tggcgtcggc gttgccacgc
     1141 cactccgcgg cacaaaggga gaacgctcga caagcttgta ttgatgcacg taccgtgggc
     1201 agttcgggta gatggccgtc gcccggacca gcaccacgaa ctgacattcc agatatctct
     1261 ccatggcatc gtgctcgaac gcgagttcag cgacgccatc cacacgcatg cgacgctgcc
     1321 gctcgaaatc gacgaacaag agaccaacgc gagcggtctg agcaacgttg cccatcgaca
     1381 gatacatgcc attgccgtcg tagttgggga acgccagggt cgccttgtcg aggatcgtca
     1441 cgaagccggg gtccccaccc ttgtatgaac aggtcggctg gccctgctga tcgaccgtcg
     1501 ccaggaagaa catgtcctgg acttggatga actgccgatc cgcatccgtg aactcatggt
     1561 ggaccttgac ctcagcgagg cggtcggcga gacggcgagt gtcgaaccgg tcctgtaggt
     1621 ttcgacttgc gtcggcgtat cggaggggca tgtatttctc ctaagaccac agagtgtaga
     1681 ttgctacgat gcttcccacg gcggtcccaa tgagcgcggt ggcaacggcg atcggatata
     1741 cgaaacctgg atgcatcctc cttcccgttg cccacacact catcgcggaa gcgactaacg
     1801 gcagtatcag cgcaagtccc gtaaatagaa gtgttcgggt tgccgtatgc gtgtagccgc
     1861 gtgtctgcac gagacgattt ccccctcgtt ccgaagccac tatgagcgcc agaccgacga
     1921 gggcacttac ggtgatcacg gtgagcagag cgagccagcg ctccccaccc cggcgccaag
     1981 ccaatacggc accggcagga aagagcagga tggccactgc atagaacaga aaaagcatgt
     2041 tgccgccctt cgctaggatt cagtgacgac agccgctgta aaagccgtcg tagccctttc
     2101 cgcttgccag ctgcagtggc ctgtgagacc gccaagcgtg cgagcccgga gtcagcgcct
     2161 caaggcagcg atgtctgggg cgtacacggt cacgaccaag gcacccccga cctccctcac
     2221 tgcatcgact gttcgtcgat ccaggcgcac ctcaaagctg tgcggttcaa gacccgcttc
     2281 gatgcccaca ttgaactcgc gcgacttggc cgaatcccag acctttcggt aacgcggcgg
     2341 gaggcgtttc acgagccgtg taagaccgat gatcgtgcga tcagccgtca tgcccacgtg
     2401 ccccgaagac gcgagctcga cgtgcgcctc gtactttcgg cccgttccac cgacgtaaag
     2461 gaccaatgct gcctcgccca tcgcatcaac gagccccgtt agggaaaccc gcgagaagat
     2521 gtcgaggtct acgtttacaa accgtgtgga ctcacacgtc agtcgaccgg tgttcttcac
     2581 agttcggttc atgcacgttg ttggcgagct aacgcatcac cagcggctgc tcacgattcc
     2641 cccagcgccg tctagtgcat gcgatctcca acttgcgttg ggtcctatgc attcggactg
     2701 ccctgcaata gtggctcgat aggacggttc tccgcgccat tctccgtgca aacaccatag
     2761 accgccgtag ggccggcata cagggcaacg cccgcatgct cgccgctggc gttcaagacg
     2821 tagaagttga ggtggaaatt tggttctccg cgactgttca agagtcgccg ttcgaccgtc
     2881 gccgatttta ctcgccgtaa tgcttcgata ccggcgtcct tgggacgcat gccacgacgc
     2941 atgctctcaa cgataagaaa cgaacacaga ccatagaggt tggcttcacc ccgcccagt
//
LOCUS       DATMED010000309         3326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4850514, whole genome shotgun sequence.
ACCESSION   DATMED010000309 DATMED010000000
VERSION     DATMED010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4850514"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VNP73_07925"
     CDS             complement(<1..1050)
                     /locus_tag="VNP73_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL65887.1"
                     /translation="MTIRKVERSVALGLILAVNIALLAPSPAAAKRTCMGRRATLVGS
                     RRAERIIGTPRNDVIVALAGADEIYARGGNDLVCGGGGRDVVLGEEDADRLDGGPGID
                     FLFGGGGNDKLELGGGLVNGGDGGDGDDSIVGNDLLRGIDLAGFSQSSDAVTVDLQAG
                     TATGNGRDTLEEIEGAVGTPKADTLLGDAGTNVLFGGSGNDSIDGRGNLGNTDSPLAE
                     EGDLLYGDGISDEPPGNDTLTGGSGLNIAVFAYSEAPVQVDLGTGTATGEGVDTLNEI
                     QFVMGSRFDDTLRGDDGDNGFDPYLGNDTIDGGEGEDVLALLAPSGVTASLETGIATA
                     NGERDTFSAIENLWGS"
     gene            1083..1712
                     /locus_tag="VNP73_07930"
     CDS             1083..1712
                     /locus_tag="VNP73_07930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWL65888.1"
                     /translation="MTRIFLVRHGATEWNVHKRAQGHADIALTDEGRRQAVHAANELA
                     HVDVDAVYSSDLQRARETARVIAESHGLDVKVDPDFREIDQGEWEGLTVDEIRRGWPD
                     LWGPARHFSARPGGETPQQVRTRALAALERAVAAHPDGTIVIVSHGGTIRWVSAETLD
                     YDDHRSARIRGLGNGGIVCITGTVHDGALDLRDYQRWDGATPDLDDPND"
     gene            1734..2921
                     /locus_tag="VNP73_07935"
     CDS             1734..2921
                     /locus_tag="VNP73_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWL65889.1"
                     /translation="MEFRRIERFPPYVFSIVNDLKTQARRSGDDVIDLGMGNPDIPTP
                     DAVVEKLKEAADNPRNHRYSASRGIPKLRSAICDLYSRAWGIELDPETEAVATIGAKE
                     GLSHLAWVLVEPGDSVLVPEPTYPIHTYSMILAGANLMSVELSADSDFFADLVQTYER
                     SEPKPRVVLCSFPHNPTTKVVDLAFFEQLVEFARAREMVVIHDLAYADLVFEEERAPS
                     LLQVPGAKDVGVEFFSMSKSYSMPGWRLAFCVGNPAIVGALTKLKSYLDYGVFQPIQI
                     AGIIALNECEHVPKQIREIYRTRRDVLCDGLDRIGWDTPRPEGTMFVWSRIPDEFQEM
                     GSLEFCMHLVREAKVAVSPGIGFGKAGEPYVRFALVENEQRIRQAVRGIRKVIAAGPP
                     VSR"
     gene            complement(2911..>3326)
                     /locus_tag="VNP73_07940"
     CDS             complement(2911..>3326)
                     /locus_tag="VNP73_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65890.1"
                     /translation="LLQESLLELRRAQQLAIAEVDDPVARIEQGIRASIEFFRRNPGF
                     LSLIRIAGRYDDFVGLVEQGHQIVVADTATHIKEGMSLGEIREGDPELLAHGVLAVIL
                     HFVETYFDGETDVVPDRPELAEEAVAFCLKGMLTG"
BASE COUNT          592 a         1070 c         1064 g          600 t
ORIGIN      
        1 agatccccac aggttctcga tggcagagaa cgtgtctcgc tcgccgtttg cggtcgcgat
       61 gccggtctcg agtgaggcag tgacgccgga gggcgccagt aaagcgagaa cgtcttctcc
      121 ctcacccccg tcgatcgtgt cgtttccgag gtaggggtcg aagccgttgt cgccgtcgtc
      181 tcccctcaac gtgtcgtcga accgtgatcc catgacgaac tggatctcgt taagcgtgtc
      241 gaccccttct cccgtcgcag tgcccgtccc gagatcgacc tggaccggag cctccgagta
      301 agcgaacacc gcgatgttca agccgctgcc gccggtgagc gtgtcgttgc cgggcggttc
      361 gtcacttatg ccgtcgccgt agagcaggtc gccttcctcg gcgagcggag agtcggtgtt
      421 cccgaggttc ccgcgaccgt cgatggagtc gttccccgac cccccaaaca gaacgttcgt
      481 tcctgcatcg ccgaggaggg tgtcggcctt gggggttccc accgcacctt cgatctcttc
      541 cagcgtgtcc cgtccgttgc cggtcgccgt tcctgcttga agatcgacag ttaccgcatc
      601 cgacgactgc gagaacccgg ccaggtcgat ccccctcagg aggtcgttcc cgacgatcga
      661 atcgtcgccg tcaccaccgt caccaccgtt caccaagccg ccgcccagct ccagcttgtc
      721 gttcccgccg ccgccgaaca ggaaatcgat ccccgggcca ccgtccaagc ggtccgcgtc
      781 ttcctcgccg agaaccacat cgcgtccgcc ccccccacac acgagatcgt tcccgccgcg
      841 tgcatagatc tcgtctgctc cggcaagggc cacaatgacg tcgttcctgg gggtcccgat
      901 gatccgctcg gctcgcctcg atccgacgag tgtcgccctg cgtcccatgc aggttcgctt
      961 cgcggccgcc ggggaaggag cgagaagcgc gatattgacg gccaggatga ggccgagcgc
     1021 gaccgacctc tcaacctttc gtattgtcac caggccccct tcgacgaacg ggagggagaa
     1081 cggtgaccag gatctttttg gtcaggcatg gggccacgga atggaacgtt cacaaacgtg
     1141 cgcagggtca tgcggatata gcactcactg atgagggccg ccgtcaagca gtacatgcgg
     1201 cgaacgagct cgcccacgtc gacgtggacg cggtttattc gagcgatctt caacgcgccc
     1261 gcgagacggc tcgggtgatc gcggaatcgc acggattgga cgtaaaggtc gatccggact
     1321 tccgcgagat cgatcagggg gagtgggagg gcctgacggt ggacgagatc cgacgagggt
     1381 ggcccgacct gtggggcccg gcccggcatt tcagcgctcg gcccggcggg gaaacgccgc
     1441 aacaggttcg aacgcgtgcg ctggccgccc tggagcgagc ggtcgcggcg catcccgacg
     1501 gcacgatcgt gatcgtcagc cacggaggga cgatccggtg ggtgagcgcg gagaccctcg
     1561 actacgacga ccacaggtcg gcgcgcatcc gcggactggg gaatggaggg atcgtctgca
     1621 tcaccgggac cgtccacgac ggagcgctcg acctgcgtga ctatcaacgg tgggacggag
     1681 ccaccccgga tctcgacgat ccgaacgact gaccggctgc tagtttgaag agcatggaat
     1741 tccgacgtat cgaacgtttc ccgccgtacg tcttctcgat cgtgaacgac ctgaagaccc
     1801 aagccaggcg ctccggggac gacgtgatcg acctcgggat gggcaacccc gacatcccca
     1861 cccccgacgc ggtcgtcgag aagctgaagg aggcggcaga caaccctcgg aaccatcgct
     1921 actcggcgtc gcggggcatc ccgaagctgc gttcggcgat ctgcgacttg tactcgcgcg
     1981 cgtgggggat cgagctcgac cccgagaccg aggcggtcgc cacgatcggg gccaaggaag
     2041 gcctctcgca cctggcctgg gtcctggttg agccggggga ctccgttctg gtgccggagc
     2101 cgacctatcc gatccacacc tattcgatga tcttggccgg agcgaacctg atgagtgtcg
     2161 agttgtcggc cgattcggat ttcttcgccg acctagtgca gacgtacgag aggtccgagc
     2221 ccaagcctcg ggtcgttctg tgctcgttcc cgcacaatcc gaccaccaag gtcgtagatc
     2281 tggcgttctt cgagcaactg gtcgagttcg cacgcgcccg cgagatggtc gtgatccacg
     2341 atctcgctta cgccgacctc gttttcgagg aggaacgtgc cccgtcgctt cttcaggtcc
     2401 ccggcgcgaa ggatgtcggg gtcgagttct tctcgatgtc gaagtcgtac tccatgcccg
     2461 gctggcgact ggcgttctgc gtcggcaacc cggcgatcgt cggtgctctg acgaagctca
     2521 agtcgtactt ggattacggc gtcttccaac ctatccagat cgccgggatc atcgccttga
     2581 acgagtgcga gcacgtaccg aagcagataa gagagatcta tcggacgcgc agggacgtct
     2641 tatgcgacgg cctggaccgg atcgggtggg acacgcctcg acccgagggc acgatgttcg
     2701 tctggagcag gatcccggat gagttccagg agatgggatc gctcgagttc tgcatgcacc
     2761 tcgtgcgcga ggcgaaggtg gcagtgtcgc ccggcatcgg gttcgggaag gccggcgaac
     2821 cgtacgtccg attcgccctc gtcgagaacg agcaacggat ccgccaagcc gtgaggggga
     2881 tcaggaaggt gatcgccgcc ggcccgccgg tcagccggtg agcatgccct tcaggcagaa
     2941 ggcgaccgct tcttcggcca gctccggccg gtccggtacg acatccgtct caccgtcgaa
     3001 gtaagtctcg acgaagtgga ggatgaccgc taggaccccg tgcgcgagca gctcggggtc
     3061 gccttccctg atctcaccga gtgacatccc ttccttgatg tgcgtagcgg tgtcggctac
     3121 gacgatctgg tggccctgct cgacgagacc gacgaagtcg tcgtaacgcc ctgcgatccg
     3181 gatcaaagag aggaagcctg ggttcctacg gaagaactcg atcgatgcgc ggatgccttg
     3241 ctcgatgcgt gccactggat catcaacttc cgcgatcgca agctgctggg cacgccggag
     3301 ctccaggagc gactcttgga ggagct
//
LOCUS       DATMED010000310         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3333189, whole genome shotgun sequence.
ACCESSION   DATMED010000310 DATMED010000000
VERSION     DATMED010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333189"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..868
                     /locus_tag="VNP73_07945"
     CDS             200..868
                     /locus_tag="VNP73_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWL65891.1"
                     /translation="MKDLESRLWTGSLSRAGGLLLALLLVVAFAFLVIQVTQEGVITR
                     FDISFAQAVNDAVWGSRALVGLALVVSFLGSPAWFYGVIGVAAIYFWRRGDRDVVLFL
                     VGTNAFGGLIDTLVKSAIDRPRPQLEHPVAEALGMSFPSGHAMATTVGYGSLLVCFLL
                     MIPSGWRKWVVAAYLVAIVLVATSRVVLGVHYVSDVVGGFVLGCAWLIACVALLHTNR
                     TFTS"
     gene            1066..1815
                     /locus_tag="VNP73_07950"
     CDS             1066..1815
                     /locus_tag="VNP73_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65892.1"
                     /translation="MAALIASFLLPAQAGSAPSPELDSRLLPVPTSTEYNHNGAQTMN
                     RYNGAQATVEVTDPDVDHNNSPDEFVASRIMAKKYRLQQWLEVGWAEVSWKDDRQYVY
                     TYSDESTKWQFHERYELVPGQSYSFRVYGCALPGDGRGTCAAIYWNGQWELLRTSKYG
                     CTYVHRKDPDRRCFIEEYTEVYSVDSSPEPTLGSDNDAVEWSDAQLLVGKTWSTWNDT
                     DYESAEGSVDSYSVCWLAKNHDFYVIKGTCS"
     gene            complement(2025..2096)
                     /locus_tag="VNP73_07955"
     tRNA            complement(2025..2096)
                     /locus_tag="VNP73_07955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2061..2063),aa:Lys,seq:ttt)
     gene            complement(2110..2427)
                     /locus_tag="VNP73_07960"
     CDS             complement(2110..2427)
                     /locus_tag="VNP73_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWL65893.1"
                     /translation="MALIIGTVLALFFLDGWLRIAVIGGAVLIEVFEIFIWLRWRGVR
                     STTGAESLVGMSAEVVSECKPRGQVQVKGQIWTAECAAGAAVGDRVTVEAVNGLTLSV
                     TPG"
     gene            2498..3019
                     /locus_tag="VNP73_07965"
     CDS             2498..3019
                     /locus_tag="VNP73_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65894.1"
                     /translation="MSKLKLLAAAFVALALVTSCGGGETTAPDAADVPEPVEEPTEEP
                     AEQDAASGSIDTCAFFSEADAKKFLGGGVTQEAKPPQGSLLGQCDYATAEGLISVQAR
                     PDTEWDATKSTFEENSEEISGLGEDNFFNSSFGLLVLTGEGYFIHVLSTQGGQGFDKD
                     SSVEIAEIVLGNI"
     gene            complement(3049..>3549)
                     /locus_tag="VNP73_07970"
     CDS             complement(3049..>3549)
                     /locus_tag="VNP73_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65895.1"
                     /translation="VTRKLSLAWEIGLPPIIVIVNEDVVARFRMESGADDFLLTSAGS
                     AEISARLGLAARRSGRGDEPTVLKVGDLTVNPENYQVYVRGRPLDLTYKEFELLKFLA
                     QRPGRVCDRDMLLQEVWGYDYYGGTRTVDVHIRRLRAKLGPEHETLIETIRNVGYRLV
                     PRPRDH"
BASE COUNT          659 a         1052 c         1134 g          704 t
ORIGIN      
        1 gcacaaggtg agtgcatcct cggccgacac ggcacccgcc gccacgtcga tacccgggtc
       61 cttgtcgagc agggcgcgcg tgaggagcct cacgtcggcg tcgtcctcca cgatgacgag
      121 gagctttgcg tttgcttcca ccgcggctag cttgcgcccc gctcttgcat cgcgcaactt
      181 ttgagaagga tctgttttcg tgaaggacct ggaaagtcgt ctatggaccg gatctctgtc
      241 gcgcgccggc gggttattgc tcgccctgct gctcgtggtg gcgttcgcat tcctagttat
      301 ccaagtgact caagagggag tcatcacgcg tttcgatatc tccttcgcgc aggctgtgaa
      361 cgacgctgtg tgggggagcc gagctctggt tggtctggcg ctggtcgtca gctttctcgg
      421 aagtcctgcg tggttctacg gcgtcatagg cgtggcggct atctatttct ggcgccgcgg
      481 ggatcgagat gtcgtgctgt ttctggtggg aacgaatgcg ttcggggggc tgatcgacac
      541 tctggtcaag agcgcgatcg accgaccccg cccccagctc gagcacccgg ttgcggaggc
      601 gctgggaatg agcttcccgt ccggccacgc gatggcgacg accgtggggt acggatcgct
      661 gctcgtttgc ttcttgctga tgatcccgag tgggtggcgc aagtgggtgg tcgccgctta
      721 cctcgtcgcc atcgttctag tcgcgacctc gcgcgtggta ctcggtgttc actacgtgag
      781 cgacgttgtg ggtgggtttg tcctgggatg tgcatggttg atcgcatgcg tcgcgctgct
      841 gcacacgaat cggactttta cgtcgtaact atttccagat agctctccct tgctagtgcg
      901 gttctgctag cgcgccatgg tcgtcctgat acgtatgggt tatgcacaac atggttagaa
      961 cgggccatag gacatcctcc ttataggtcg tattacatag gcagagcgga acgctaggga
     1021 ggcttccgaa tggtcctaag aagtagtcac acgatccggg tcgcaatggc tgcgctgatc
     1081 gcatccttct tgctgcccgc gcaggcaggt tcggcaccgt cgcctgagct cgattcccgg
     1141 ctccttccgg ttccgaccag caccgagtac aaccacaacg gcgcgcagac gatgaaccgc
     1201 tacaacggcg cgcaggcgac ggtcgaggta acggatcccg acgtcgatca caacaactca
     1261 ccggatgaat tcgtcgcctc gcggatcatg gcgaagaagt accgcctgca gcagtggctg
     1321 gaggtcggct gggccgaggt cagttggaaa gacgacaggc agtacgtcta cacctacagc
     1381 gacgagagca cgaagtggca gttccacgaa cggtacgagt tggtgccggg tcagtcctac
     1441 agcttccgcg tgtacgggtg tgctcttccc ggcgacggcc gcggaacgtg cgcggcgatc
     1501 tactggaacg ggcaatggga actgctgaga acgagcaagt acggctgcac ttacgttcac
     1561 aggaaggacc cggatcgccg ctgcttcatc gaggagtaca ccgaggtgta ttcggtcgat
     1621 tcgtcaccgg aacccactct cggctccgac aacgatgcgg tcgagtggtc cgacgcacaa
     1681 ctactcgttg gcaagacgtg gtcgacctgg aacgacaccg actacgaatc ggcggaagga
     1741 agcgtcgact cctacagcgt gtgctggctt gccaagaacc acgacttcta cgtgatcaaa
     1801 gggacgtgtt cttaatggct gcgagacggg tctgcgactg gccccgccag ggactcgccc
     1861 ttccgtccgt tcgtcggctt caaggaccaa gtcgacggga cggaagggct gggtcccgct
     1921 taatcaggat ccaacgcgtt caccgttcgg tgaaccgcca ccggcgctag tcggccacgc
     1981 gtcgggcatg gcgagggggc acacacaagt gggcacacac gaagtgggcc cccagggact
     2041 cgaaccctgc ccgccggatt aaaagtccgg agctctacca gatgagctag gggcccgtct
     2101 gggccgaggc taaccgggcg taacgctgag ggttaggccg ttcaccgctt cgacggttac
     2161 gcgatcgcct acggccgcgc cggccgcgca ctcggcggtc cagatctgac ccttgacctg
     2221 cacctggccg cgcggcttgc actcgctgac cacttcggca gacatgccca cgaggctctc
     2281 cgctcccgtc gtcgatcgga cgccgcgcca gcgaagccaa atgaagatct cgaacacttc
     2341 gatcagcact gccccaccga tcacggctat gcgcaaccag ccgtctagga agaagagcgc
     2401 caggaccgtg ccgatgatga gtgccatgca gccatcatcc tcccttggtg attcgagttg
     2461 tagcctgccc acgaacagac gacctgaggg ggtcccgatg tcaaagttga aactgcttgc
     2521 cgctgcgttc gttgcacttg cactcgtaac ctcttgcggg ggaggcgaga ccaccgcacc
     2581 ggatgcggcg gatgttcccg aacccgttga agaacctacc gaagagcccg ccgagcaaga
     2641 tgctgcgagc ggcagtattg acacctgcgc gttcttctcc gaagccgatg ccaagaagtt
     2701 cctgggggga ggcgtcactc aggaggcgaa accgccccag ggatctctgc tggggcagtg
     2761 cgattacgcg accgctgagg ggttgatttc ggtgcaggcg agaccggata ccgagtggga
     2821 cgcgaccaaa tcgacgttcg aggagaatag tgaggagata tcgggtctcg gcgaagacaa
     2881 tttcttcaac tcttcattcg ggctgctcgt acttaccggt gagggctact tcatccacgt
     2941 cctgtccacg caaggaggtc agggatttga caaggactcg tccgtcgaga tcgctgagat
     3001 cgtgctgggc aacatctagg gtctcgacgc gctaggtcgt ccgctttgct agtggtcgcg
     3061 tggacggggg acgaggcggt agccgacgtt gcggatggtc tcgatcaaag tctcgtgttc
     3121 ggggccgagt ttcgctcgca ggcggcggat gtggacatca acggttctcg taccgccgta
     3181 gtagtcgtag ccccacacct cctgcagcaa catgtcgcgg tcgcacaccc tgcccggtcg
     3241 ttgcgcgagg aacttcagta gctcgaactc cttataggtg aggtcgagag ggcggccgcg
     3301 cacgtacacc tggtagttct cggggttgac ggtgaggtcc ccgaccttca gtaccgtcgg
     3361 ttcgtcgccg cggccgcttc ggcgggcggc gagaccgaga cgagcggaga tctccgcgct
     3421 cccggcggag gtcaggagga agtcgtcggc tccggactcc atgcggaagc gagcaacgac
     3481 gtcctcgtta acgatcacga tgatcggtgg aaggccgatc tcccatgcga gcgacagttt
     3541 cctcgttac
//
LOCUS       DATMED010000311         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1789246, whole genome shotgun sequence.
ACCESSION   DATMED010000311 DATMED010000000
VERSION     DATMED010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789246"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VNP73_07975"
     CDS             <1..986
                     /locus_tag="VNP73_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HWL65896.1"
                     /translation="PYASVADGTNVIDLQGMARDCTHRVALTVALPGEEEIELEVHVV
                     RGATDGPTVGAFSGIHGDEQVGVAALARLAETLGADKIRGTMILMPIVNPPATWAGSR
                     LIPNEATDLNRSFDASLHRGPIAGAIADALLSVVTKLDFLYTMHSWSSGYACLPYIEH
                     QTGARVVSASRSAADAMGLGFVEPIDPKPGRLLTAAARAGVPAIEGEVGGLGMTTLAL
                     ERSYEGTVLRLAAHLGMTRHLSRPRRPAVHIVRHDVTSPLDAILRLGPSLGAGVAQDE
                     GIAEIVEPASGMRRILKSPAGGWLGIRRETATVGAGVLVASIFAPLRGGRS"
     gene            986..1900
                     /locus_tag="VNP73_07980"
     CDS             986..1900
                     /locus_tag="VNP73_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="phosphotriesterase homology protein; PhP; YhfV;
                     member of a family of proteins related to
                     phosphotriesterase (PTE); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HWL65897.1"
                     /translation="MASIQTVLGPVDSSRLGVCLPHEHVFLNLMSEHGPDGRLNDLHL
                     MTEELARFHALGGGALVELTSIGLGREPVMLRQVASATGLHIIMGTGYYRDPYLEHTE
                     LDAEGTNACADRLIGEVRDGIDGTDIHPGIIGEIGSGRTISPREERSLRAAARAHLAT
                     GLTISTHAAGWPVGLEQLNLLAEEGVTAGRVIIGHADTVPSTDYHEAIAARGAYVEFD
                     NLQGVTEYDTQTVIRFILTLRSLGHLDRILLSHDICLRSQLSIYGGPAYSFLLKDFVP
                     RLLAAGLSTREIEILTVENPAAAISGER"
     gene            1969..>2237
                     /locus_tag="VNP73_07985"
     CDS             1969..>2237
                     /locus_tag="VNP73_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65898.1"
                     /translation="MSQVRVLVLGATPENPAPGLPEETTGVAYDFASTVEEAMALAPT
                     ADVAFLWEWHTPLLEQMWPFTTRLKWVHVIGVGIEWALFPALVES"
BASE COUNT          412 a          683 c          732 g          410 t
ORIGIN      
        1 aaccatacgc gtcagtcgcc gatggcacga acgtgatcga cctgcagggc atggctcggg
       61 actgcaccca tcgggtggcg ctgaccgttg cgctacccgg agaggaggaa atcgagctcg
      121 aggtccacgt ggtgcggggc gcgacggacg ggccgacggt gggtgccttc tcgggcattc
      181 acggcgacga gcaagtcggg gtggcggccc tggcgcgact ggccgagacg ctgggtgccg
      241 acaaaatacg cgggacgatg atcctgatgc cgatcgttaa cccgccggcg acttgggccg
      301 gatcgcgtct cataccgaac gaagcaaccg acttgaaccg ctcgtttgac gccagcctac
      361 accgcggccc catcgcgggc gccatagcgg atgcactgct ttccgtcgtg actaagttgg
      421 acttcctgta cacgatgcac agttggagct caggatatgc gtgcctcccc tacatcgagc
      481 accaaacggg tgcgagggtc gtcagtgcga gccgctcggc cgctgatgcc atggggctgg
      541 gatttgtgga accgatcgac cctaaaccgg gacggctact cacagcggcg gcgcgcgccg
      601 gagtgccggc gatcgaaggc gaggtcggcg ggctgggcat gacaaccttg gcgctggagc
      661 gctcatatga gggcacggtt ctacgtctgg cggcacatct cggaatgacg cggcatctct
      721 ctcgaccgag gcgaccagcg gttcacatcg tgcgccacga tgtcaccagc ccgctggacg
      781 ccatcctgcg actcggcccg agtctcggcg ctggtgttgc gcaagatgag ggaatcgcag
      841 agatcgtcga acctgcgagt gggatgcgac ggatcctgaa gtctcctgct ggcggatggc
      901 tcggcattcg tcgcgagacc gcaactgttg gggcgggcgt tcttgtcgcg agcatcttcg
      961 ctcccttgcg cggaggccgg tcgtaatggc ttccatacaa acagtgttgg gtccggtcga
     1021 ttcgtcgcgc cttggggtgt gcctgcctca cgagcacgtg tttctcaacc tcatgagcga
     1081 gcatgggccg gacggccgtc tcaatgactt gcatctcatg accgaggagc tcgcccgttt
     1141 ccacgcgctg ggcggcggcg cactcgtcga actgacaagc atcggcctcg gccgggaacc
     1201 agtcatgctg cggcaggtag cgagtgcgac tggccttcac atcatcatgg gcactgggta
     1261 ctaccgcgac ccttacctcg agcacacgga gctcgatgca gagggcacga acgcatgcgc
     1321 tgatcgcctc attggcgagg ttcgggacgg catcgacggt accgatattc acccagggat
     1381 catcggcgag atcgggagtg gacggaccat cagtccgcgt gaagagcgct cgctgcgggc
     1441 agctgctcgt gcgcatctag cgacgggtct aaccatcagc acccacgctg cgggatggcc
     1501 ggtcggcttg gagcagttga atctgctcgc ggaagaaggc gtgacagcgg ggcgcgtcat
     1561 cattggccac gcggacaccg tgccgagcac cgactaccac gaggccatcg ccgcgcgggg
     1621 tgcctacgtg gagttcgaca acctccaggg cgtcaccgag tacgacactc aaactgtcat
     1681 ccgattcatc ctgacgcttc ggtcgctcgg ccacctagat cgcattctcc tgtcgcatga
     1741 catctgcctg cgatcccagc tctcgatcta cggaggcccg gcgtattcgt tcctcctcaa
     1801 agacttcgtc ccacgattgc tggcggcggg cctgtcaact cgagagatcg agatattgac
     1861 tgtcgagaac cccgcggcgg cgataagcgg ggagcgttga ttgtaagacg atcatatgag
     1921 aagatcgctg cggtgggttc agaagctgcg tgcgccagag gagggtcgat gtcacaggtc
     1981 cgcgtcctcg ttcttggggc aactccggaa aaccccgccc caggcctgcc ggaagagacg
     2041 accggtgtcg cctatgactt cgccagcacc gtcgaggaag cgatggcact cgcgccaacg
     2101 gccgatgtgg cgttcctctg ggaatggcac acgcccctac tggaacaaat gtggcctttc
     2161 acgacacgcc tgaagtgggt tcatgttatt ggggtcggta tcgagtgggc cctcttccca
     2221 gcccttgtgg aaagcga
//
LOCUS       DATMED010000312         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5625943, whole genome shotgun sequence.
ACCESSION   DATMED010000312 DATMED010000000
VERSION     DATMED010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5625943"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="VNP73_07990"
     CDS             complement(<1..1651)
                     /locus_tag="VNP73_07990"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWL65899.1"
                     /translation="MAPEAKSVERKIGDKVFRIETGKVAGQASGAVIASAGETTVFAA
                     ATSAPPRGAADFFPLTVDVEERMYAAGKIPGGFFRREGRASEKAILNARLIDRPIRPS
                     FPDGFRHEVHIVVTSWAVDNSNPMDVLAVNAASAALHVSDIPFEGPVGCVRLSHIDGE
                     WVANPTYEEQEDETIELVVAGRQNEAGEVDIMMIEAGASDRLFELIDEGARAPEEELL
                     AEGIEQSKQFIAEVIAMQNELRELAGKPKGATEGDDPEYPLVVDYEEELYERIKAEAE
                     DKLRKAVSIIEKGPRRKALYDIAQEITEKLAEDEPENAVPIQNAIRSLTKKLVRKRII
                     DEGTRLDGRKTDEVRPIWSEIGAIPRVHGSGLFTRGETQAMSICTLGMQRMEQMVDDL
                     SLDESKRYMHHYNFPPYSTGEAGFMRGPKRREIGHGALAEKALLPLIPSKEDFPYAIR
                     VVSEILSSNGSTSMASVCGSSLALMDAGAPLRGGKHCGGVAMGLIHDEGKFVPLTDIL
                     GDEDAFGDMDFKVAGTEDVVTALQLDTKIKGIPAEVLRAALM"
     gene            complement(1818..2087)
                     /gene="rpsO"
                     /locus_tag="VNP73_07995"
     CDS             complement(1818..2087)
                     /gene="rpsO"
                     /locus_tag="VNP73_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWL65900.1"
                     /translation="MALIKEEKQKIITENATAEGDTGSPEVQVALLTRRINELTEHLK
                     THSKDHHSRRGLLRMVGRRRRLLEYLRREDVDRYRALIAKLGLRR"
     gene            complement(2151..3104)
                     /locus_tag="VNP73_08000"
     CDS             complement(2151..3104)
                     /locus_tag="VNP73_08000"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWL65901.1"
                     /translation="MRRVLGIENLSPPPNGCAVAIGTFDGVHLGHRALIDAAKRRAER
                     EGLASAILTWDRHPNETVHPDRIPPLLTSTERRIELLSEQGADLLAVLEFNRELSSWS
                     PERFVKEVLANGLSTRVVTIGEGWRFGRGAAGTVELLEELGSDLGFEVEVVPLQVVDG
                     EPVSSSRVRSIVASGDMQGAEKLLQRPFDVEGVVVHGDDRGAGLGFPTANVALHPLLA
                     SPARGVYAGRARADEKWHTAAINVGVNPTFGGDPSSMPMRIEAFLLDFSGGLYDETIR
                     IEFHERLRDEERFPSADALVEQMKLDVEATRRVIESRRDSC"
BASE COUNT          536 a         1052 c          959 g          650 t
ORIGIN      
        1 gcatcagtgc ggcgcgtagg acctctgccg ggatgccctt gatcttcgtg tcgagctgca
       61 gagcggtaac gacgtcctcg gtaccggcaa ccttgaagtc catgtcgccg aaggcgtcct
      121 cgtcaccgag gatgtccgtc aggggaacga acttgccctc gtcgtggatc aggcccatcg
      181 caacgccacc gcagtgcttg ccaccgcgga gcggcgcacc ggcgtccatc aatgccagcg
      241 aagaaccgca cacactggcc atcgatgtcg agccgttgga cgacaggatc tcggacacga
      301 cgcggatggc gtacgggaaa tcctctttgc tcgggatgag aggcagcaat gccttctcgg
      361 ccagagcgcc gtggccgatc tcgcgccgct tcggcccccg catgaagccc gcctctccgg
      421 tggagtaggg cgggaagttg tagtgatgca tgtagcgctt ggattcgtcg agcgacaggt
      481 cgtcgaccat ctgctccata cgctgcatac caagggtgca gatagacatc gcctgggtct
      541 ctcctcgggt gaacagaccc gatccgtgaa cacgcgggat cgcgccgatc tcgctccaga
      601 tgggacggac ctcgtccgtc ttccgcccgt cgagcctcgt gccttcgtcg atgatccgct
      661 tgcgtacgag cttctttgtc agtgaccgga tcgcattctg gatcgggacc gcattctcgg
      721 gctcgtcctc ggccagcttc tcggtgatct cctgcgcgat gtcatacagc gccttgcgac
      781 gagggccctt ttcgatgatc gagacggctt tgcgcagctt gtcttcggcc tcggccttga
      841 tccgctcgta gagctcttcc tcgtagtcga cgaccagcgg gtactcagga tcgtcacctt
      901 cggtagcgcc cttgggtttg cccgcgagct cacgcagctc gttctgcata gcgatgacct
      961 cggcgatgaa ctgcttcgat tgctcgatgc cctcggccag caattcctcc tcgggagcgc
     1021 gcgctccctc gtcgatcagc tcgaagagtc tgtccgacgc tccggcctcg atcatcatga
     1081 tgtcgacctc gccggcttcg ttctggcggc cggcgacgac caactcgatc gtctcgtcct
     1141 cttgttcctc ataggtcggg ttcgcgaccc actcgccgtc gatgtgcgag aggcgcacgc
     1201 aaccgacggg tccctcgaag gggatgtccg agacgtgcag ggccgcggat gctgcattga
     1261 cggccaggac gtccatcggg ttgctgttgt cgacggccca cgacgtcacg acgatgtgga
     1321 cctcatgccg gaacccgtcc gggaacgacg ggcgtatcgg ccggtcgatc aagcgggcgt
     1381 tcaggatcgc tttctcggag gctctgccct ctcgcctgaa gaacccaccc gggatctttc
     1441 cggccgcgta catacgttct tcgacgtcca cggtgagcgg aaagaagtcg gcagccccgc
     1501 gtggcggagc cgatgttgct gcggcaaaga ctgtggtctc acctgcggat gcgatgaccg
     1561 ctccggatgc ttgacccgcg accttgccgg tctcgatgcg aaataccttg tcgccgatct
     1621 ttctttcgac ggacttggct tctggtgcca tgttttcctc ctaacaaatt tcggttagga
     1681 gaggcgccgg tcctttgtgg ccgattctca gttgtaagac cacatcgaag atgtgtgggc
     1741 ttgcaactga taaccgaccg ccggcggccc cctcccttac taactgcttc gttcgatcct
     1801 aggggcggcc ccttttccta tctgcgaaga cccagtttcg cgatgagggc tcggtaacga
     1861 tcgacgtcct cacgcctcag gtactcgagg agtctccgtc tgcggccgac catgcgtagg
     1921 aggccgcgcc gtgagtggtg gtccttggag tgagtcttga ggtgctcggt gagctcgttg
     1981 atccggcgcg tgagaagagc gacctggacc tcgggcgaac cggtgtcgcc ctcggcggtc
     2041 gcgttctcgg tgatgatctt ctgcttctct tctttgatca aagccatgcg cgtcgaatga
     2101 gaccttcttc tagttgatta ccggggcaac gtcccccagc gccgctcatc ctaacaggag
     2161 tcccgccggg actcgatgac ccgcctggtg gcctcgacat ccaacttcat ctgctctacg
     2221 agggcgtcgg ctgaggggaa tctctcctcg tcccggaggc gctcgtggaa ctcgatcctg
     2281 atcgtttcgt cgtagagacc gcccgagaag tcgagcagga aggcctcgat ccgcatcggc
     2341 atcgacgagg gatcgccccc gaaagtgggg ttgaccccca cgttgatggc tgccgtgtgc
     2401 cacttctcgt cggcacgggc tcgacctgcg tacacgcctc gagccggcga ggccaataac
     2461 ggatgcaggg cgacgttcgc agttggaaaa ccgaggccgg caccccgatc gtccccgtga
     2521 acgaccacac cttcgacatc gaagggccgc tgcagcagct tttcggcccc ttgcatgtcc
     2581 cccgacgcga ctatggagcg aaccctgctg gacgaaaccg gctcgccgtc gaccacctgg
     2641 agggggacga cttccacctc gaaacccaga tcggagccga gttcctcgag tagctcgacc
     2701 gtgccggccg ctccccgtcc gaaacgccac ccctcaccga tcgtcacgac gcgcgtcgag
     2761 agaccgttcg ccagcacctc tttcacgaat cgctccggcg accacgacga gagctctcgg
     2821 ttgaattcca gaacggccag cagatcggca ccctgttcgc tcaacagttc gatcctgcgc
     2881 tccgtgctgg tgagcaaggg cgggatccgg tcgggatgga cggtctcgtt cgggtgccgg
     2941 tcccacgtca ggatggccga cgccagccct tctctttcgg cgcgtctctt cgcggcgtcg
     3001 atcagtgccc tatgcccgag atggacgccg tcgaaggtcc cgatcgcgac cgcgcagccg
     3061 ttcggaggag gagacaggtt ctcgatcccg agcacccttc tcatttcgtc ctctctccag
     3121 ccgtcagaac gcggtcggga atcagagctc ccccgtctgc gcgatacacc gccaccaagt
     3181 ctccatccga aacgacg
//
LOCUS       DATMED010000313         5542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_643486, whole genome shotgun sequence.
ACCESSION   DATMED010000313 DATMED010000000
VERSION     DATMED010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643486"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..928)
                     /locus_tag="VNP73_08005"
     CDS             complement(35..928)
                     /locus_tag="VNP73_08005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWL65902.1"
                     /translation="MYPAPELSGTHRALATLITERLRLLLERRSIRFAVVGFLLIAAS
                     TFTFFPAPVQAVDFDPRWIFILPVAIAAIAAGLREGLTVALISVALIGLFAAAATGGA
                     YGEAALVTLVAQWFALLGIVAAVLGAFAEAHYSVQSSLMQLATTDPLTKVSNIERFYH
                     ELSVLQASKTPYVVMILDLDELKTLNDQHGHQAGSMAIQTVANVLRSVVRGTDTIARY
                     GGDEFVVILKDADRVGAQIVTNRVRTLLADQKLPQAPEALLTVSMGVAVAGEDGATSE
                     ELLSVADKAMYEDKRSRKMAV"
     gene            complement(1014..1613)
                     /locus_tag="VNP73_08010"
     CDS             complement(1014..1613)
                     /locus_tag="VNP73_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65903.1"
                     /translation="MARTHARTVLALVVALWTVLVWSLTVAAGPNDDPVAARTHCETI
                     PVVEEKVCNPTESDHRETDDDTPPPSSSRPPSSATPSGSASPSGSASPSGSSSPTSPP
                     SRSYDSQITIRYRNGRFFGVVESSSERCWSRRSVNLKEKNNGRSVTAGADATDRSGSW
                     AVRFPRARGRFFATVNVERIQTRGGEIRCEGARSRTISR"
     gene            complement(1645..2556)
                     /gene="ligD"
                     /locus_tag="VNP73_08015"
     CDS             complement(1645..2556)
                     /gene="ligD"
                     /locus_tag="VNP73_08015"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWL65904.1"
                     /translation="MTKSAKIEMRVGGKTVSISNPDKVYYPETGFTKGDVVSYYRAVA
                     PALMPHLKGRPLTLKRYVDGVQGDFFYEKRCPPHRPEWVRTVAVRRKRDGKDIDFCVV
                     ENQATLLWAANLASIELHTGLAKARALRRPNFLVFDLDPGPPADVLDCAHVAVELKKS
                     LEAVGLESFCKTSGSKGLQLFVPLNSAVTFDDTRTYAHSLARTLEEAHPEEIVTEMKK
                     ELRTGKVFIDWSQNAEQKTTVCVYSLRAREHPTVSTPVSWREITSALRKDDAESLVFE
                     AKEVLKRIDKNGDLFKPVVAKKQKLPV"
     gene            2573..3613
                     /locus_tag="VNP73_08020"
     CDS             2573..3613
                     /locus_tag="VNP73_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWL65905.1"
                     /translation="MQREHEGPSTVTIFRVVFTVAAALLVLYGLYLVRSVLLLIAVGG
                     FLAVGLDPAVRRLQGWGFKRGQAIGVILLAAIVVLVGFFAAVVPPLVEQVTRFATDLP
                     DYIRQLAEKNPRIEEYVTEQDIAARLQAATSSLPNTITGSFGTVLGFAGSIVGTIFTT
                     LTVIVLTIYFSSSLEGIRAGTFKLVPRSRRDRVQELADPILTKIGSYIAGNVVISVIA
                     GVVSFVFLLIAGVPFPVALALWVAIADLIPMVGATLGAVPAVVVAFFTSGPLGIATLI
                     FFVIYQQVENYVVAPRVMTKAVDLSPAAVLLSALAGAALLGVMGVLMAIPAAAAAKLI
                     VQEVVVPRAEEA"
     gene            3616..4410
                     /locus_tag="VNP73_08025"
     CDS             3616..4410
                     /locus_tag="VNP73_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWL65906.1"
                     /translation="MVVEQTREVRVSDLRPLDTEEAERISNEMSRALVQLLKELDVSE
                     WDSPTDCDRWTVRDHVSHQIGWNEALISTRELFHQMSSAAGRVKEIGNIIDAQNQVQV
                     DERRHLEPPQAIACLEETFPRGAAKRKRLSSILGRVPIYSAYLGGWMKVEYLAAAIFP
                     RDIYMHRVDIAQATGREVKIGPAETRLIDDIVRDWFARTKTAVRLELTGPAARSYVSD
                     NAPLATLRVDAVQFTRVLFGRADRSIIEIEGDRTIADRALDTFFPV"
     gene            complement(4407..4880)
                     /locus_tag="VNP73_08030"
     CDS             complement(4407..4880)
                     /locus_tag="VNP73_08030"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWL65907.1"
                     /translation="MTELFKTIVSSPIGDLVLAGDGRKLHRINLPGGNWPEPDAIEDD
                     SKFDKVVGQLEEYWDGARKTFDLDLYPQGTDFRMRVWSALGDIPYGRTVGYGELARKI
                     GRPKAARAVGAACGANPLPIVIPCHRVIGASGTLTGFGGGLDMKAQLLKLEGWER"
     gene            complement(4877..>5542)
                     /locus_tag="VNP73_08035"
     CDS             complement(4877..>5542)
                     /locus_tag="VNP73_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017545895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWL65908.1"
                     /translation="SAVDRSRRLLDLDADPDAVAGTLRRDPVLKDRGPFKKGTRLPGT
                     VDGFEIAARAILGQQVSVAAARKLASRLVGLCGDVLAQPDHGLTHRFPTPRRVVAADL
                     GGMGVPSRRLATLKSVALRAAEGRLDLDAGADRTELQRSLVEIPGVGPWTASYIAMRL
                     GDPDVFLSDDLGVKRALGSSSPVESEELAANWSPWRSYAVLHLWNSTTRNGKEPAKRR
                     SAS"
BASE COUNT          981 a         1789 c         1692 g         1080 t
ORIGIN      
        1 gaccggatgg tactgacttt ttgctagtcg gcccttagac ggccatcttc cgagagcgct
       61 tgtcctcgta catggccttg tcggcaaccg agagcagctc ttcggatgtc gctccgtcct
      121 caccggccac ggccacaccc atgctgacgg taagtagcgc ctcgggggct tgcgggagct
      181 tctgatctgc gagcaaggtc cgcacgcggt tcgtgacgat ctgagccccg acgcggtcgg
      241 catccttgag gatcacgacg aactcgtcac cgccgtaccg ggcgatcgtg tcggttccgc
      301 gcacaacact tcggagcacg ttcgcgaccg tctggatcgc catcgaaccg gcttgatgac
      361 cgtgctggtc attcagtgtc ttgagctcgt cgagatcgag gatcatcacg acgtatggag
      421 tcttgctcgc ctgtaggacg gatagttcgt gatagaagcg ctcgatgttc gacaccttgg
      481 tcaacggatc cgtggtggcg agctgcatga ggctcgactg cactgaatag tgcgcttctg
      541 cgaacgcacc caggacggcg gcgacgatcc cgagcagcgc gaaccactgc gccaccagcg
      601 tcaccagcgc ggcctctccg tacgcacctc cggttgccgc cgctgcaaat agaccgatca
      661 acgcgaccga tatgagagcg accgtgagac cttctctgag gcccgctgcg atcgcggcga
      721 tagcgacggg caggatgaag atccagcgag ggtcgaaatc gaccgcttgc accggagccg
      781 ggaagaacgt gaacgtgctg gcggcgatca gtaggaatcc gacgaccgcg aacctgatcg
      841 accttcgctc cagaaggagc cgaaggcgct cggtgatcag ggttgcaagc gcgcgatgag
      901 tgcccgagag ctcgggcgct gggtacatgg ccttcaagtc gtccttcttc tcccccccga
      961 ggggcgtcct tattcagata tcggaccatg aagacaccca cttgagaagg accctagcga
     1021 gagatcgttc gtgacctggc gccctcgcac ctgatctcgc cgccccgggt ctggatgcgc
     1081 tcgacgttca ccgtggcgaa gaagcgcccc cgagctcgag ggaaccgcac ggcccaggag
     1141 cccgaccgat cggtcgcatc cgctccggcc gtcaccgaac gcccgttgtt cttctccttc
     1201 agattcacgg atcgccggct ccagcatcgc tcgctactcg actcgacgac cccgaagaag
     1261 cgcccgttcc ggtacctgat ggtgatctgc gagtcgtagc ttcggctcgg aggactcgtc
     1321 ggggaggagc ttcccgaagg gcttgctgat cccgagggcg aggcagaacc actcggggtc
     1381 gcgctgctgg ggggccgcga gctcgaaggt gggggcgtgt cgtcgtcggt ctcgcgatga
     1441 tcgctctcgg tgggattgca aaccttctcc tcgaccacgg ggatcgtctc gcagtgggta
     1501 cgggccgcga ccgggtcgtc gttaggaccg gctgcaacgg tcagagacca aaccaaaacc
     1561 gtccacagcg cgaccacgag cgcaagaacc gtcctcgcgt gcgttcgcgc catcgatctc
     1621 ctcccctaca gcgtaagtcc gaccctacac ggggagcttc tgtttcttgg ccaccacagg
     1681 cttgaataga tcgccgttct tgtcgatcct cttcaacact tccttcgcct cgaacacgag
     1741 tgactcggcg tcgtccttgc gtagcgcgct cgtgatctct cgccatgaca caggcgtcga
     1801 caccgtcgga tgctcgcggg cgcgcagcga atacacgcaa accgtcgttt tctgctctgc
     1861 gttctgactc caatcgataa agacctttcc tgttcgaagc tccttcttca tctctgtgac
     1921 gatctcttcc gggtgcgctt cctcgagcgt tcgtgcgagc gaatgcgcat acgtccgcgt
     1981 gtcgtcgaaa gtgaccgcgc tgttgagcgg tacgaagagt tgcagcccct tggacccaga
     2041 cgtcttgcag aaggactcca ggcccacagc ctcgaggctc ttcttcaact ccacggccac
     2101 gtgggcgcaa tccaatacgt ctgcgggagg gccgggatcg agatcgaaaa caaggaagtt
     2161 cgggcgcctc aaagcgcgag ccttggcaag tcccgtatgg agctcgatgc tggccaggtt
     2221 cgcggcccac agaagtgtgg cttggttctc gaccacgcag aaatcgatgt ccttgccgtc
     2281 cctcttgcgg cggacggcaa cggtgcggac ccattccgga cgatgaggcg ggcacctctt
     2341 ctcgtagaag aaatcaccct ggacgccgtc gacgtagcgc ttgagggtga gcggccgccc
     2401 cttgaggtgc ggcatgagag cgggggccac agctctgtaa taggagacga cgtcgccctt
     2461 ggtgaagccc gtctctgggt aatagacctt gtcggggttt gagatagaga cggttttccc
     2521 gcccactcgc atctcgatct tggcggactt tgtcatcggg cagaatctaa ctatgcagcg
     2581 cgagcacgag ggaccctcaa cggtcacgat cttcagggtc gtgttcacgg tcgccgccgc
     2641 cctgctcgtc ctctacgggc tctacctggt ccgctccgtg ctgttgctca tcgccgtggg
     2701 tgggttcctt gcggtcggat tggatccggc cgtgcgacgg ctccagggct ggggattcaa
     2761 acgggggcag gcgatcggag tgatcctgct cgccgcgatc gtggtgctgg ttggcttctt
     2821 cgccgccgtc gttcctccac tcgtcgagca ggtcacgcgc ttcgctaccg atctcccgga
     2881 ctacatacgt cagctggccg agaagaatcc acgcatcgag gagtacgtga ccgagcagga
     2941 catcgccgcg cggctccaag ctgcgaccag cagtctcccg aacaccatca ccggatcgtt
     3001 cggtaccgtg ctcggcttcg cgggttcgat cgtcgggacg atcttcacta ccctcaccgt
     3061 catcgtgctg acgatctatt tctccagcag cctcgagggg atcagagccg gcacgttcaa
     3121 gctggtgcct cgcagccgga gggatcgtgt ccaagaactc gccgacccga tccttacgaa
     3181 gatcgggagt tacatcgccg gcaacgtcgt gatctccgtg atcgcaggcg tcgtctcctt
     3241 cgtattcctt ctgatcgcgg gggttccgtt cccggttgct cttgctctat gggtcgcgat
     3301 cgccgacctc atcccgatgg tgggtgcgac gctcggagcg gttccggccg tcgtcgttgc
     3361 gttcttcacg tcgggtccgc tcgggatagc aacgttgatc ttcttcgtca tctaccaaca
     3421 ggtggagaac tacgtggtcg ctccgcgcgt gatgaccaag gccgtcgatc tgtcacccgc
     3481 agcggttctg ctgtcggcac tcgccggcgc cgcattgctg ggagtcatgg gtgtactgat
     3541 ggcgatccct gcggccgcgg cggcaaaact gatcgtccag gaagttgtgg tccccagggc
     3601 cgaggaggca tagcaatggt cgttgaacaa acgcgagaag tccgggtctc cgatctgcga
     3661 cctctggata cagaggaagc ggaacggatc tcgaacgaga tgagccgggc cctggtccag
     3721 ctcctgaagg aactcgacgt gagcgagtgg gactccccca ccgactgcga tcgctggacc
     3781 gttagggatc acgtctctca tcagatcggc tggaacgagg cgctgatctc gacccgtgag
     3841 ttgttccacc agatgtcgtc ggcggcgggc cgcgtgaagg agatcgggaa catcatcgat
     3901 gctcagaacc aggtgcaggt cgacgaacgc aggcacctcg aaccacctca agcgatcgcc
     3961 tgtctcgaag agacgttccc gcgaggcgcg gccaagagga agcggctgag ctcgatcctc
     4021 ggccgggtgc cgatctacag cgcctacctc ggtggctgga tgaaggtcga gtacctcgca
     4081 gctgcgatct ttcctcgtga catctacatg caccgcgtcg acatcgctca ggccaccggc
     4141 cgagaggtca agatcggtcc ggcggagaca cgactgatcg acgacatcgt cagggattgg
     4201 ttcgctcgca cgaagactgc tgtccgattg gagctgaccg ggccggcggc ccgttcatac
     4261 gtgagcgaca acgctccact tgccacgctt cgcgtcgacg ccgttcaatt cacgcgtgtg
     4321 ctgttcggtc gcgccgaccg gtccatcatc gagatcgagg gagaccggac catcgccgac
     4381 cgggccctgg atacgttctt tccggtctag cgctcccagc cttcgagctt caagagctgc
     4441 gccttcatat ccaagccccc gccgaatccg gtaagcgtcc cgctcgcgcc gatcacgcgg
     4501 tggcacggga tcacgatcgg cagcggattc gcgccacacg cagcccctac tgcgcgcgct
     4561 gccttcggcc gtccgatctt ccgggcgagc tcgccgtatc cgacggttcg gccgtagggg
     4621 atatcgccga gcgccgacca cacccgcatc ctgaagtcgg tcccttgcgg atagagatcg
     4681 agatcgaacg tcttcctggc cccgtcccag tactcctcga gctgcccgac caccttgtcg
     4741 aacttggagt cgtcttcgat cgcgtccggc tcgggccagt tgcccccggg aaggttgatg
     4801 cgatgaagtt tgcggccgtc gccggccagg acaaggtcgc cgatgggact cgacacgatc
     4861 gtcttgaaca actcggtcac gatgcactcc ttctcttagc aggttcctta ccgttccgtg
     4921 tcgtgctgtt ccagagatgc aggacggcgt agctgcgcca cgggctccag ttcgcagcga
     4981 gctcttccga ttcgacgggg ctcgacgagc cgagtgcccg cttcaccccg aggtcgtcac
     5041 taaggaagac gtccgggtcg cccagccgca tcgcgatgta cgacgccgtc cacggaccga
     5101 cccccggtat ctcaacgagg ctgcgctgca actccgttcg gtccgcccct gcatccaggt
     5161 cgagtcgccc ttcggctgcc ctgagtgcaa ccgatttcag cgttgcgagc cgcctgctgg
     5221 ggacacccat ccccccgaga tcggcggcca cgacccgtct gggtgtcggg aatcgatgcg
     5281 tcaatccatg atccggctga gcgagcacat ctccacacag acccacgagg cgactagcaa
     5341 gcttgcgagc ggccgcgacc gatacctgtt gtcccaagat cgcccgtgct gcgatctcga
     5401 atccatccac ggtgccggga agtcgcgtcc ccttcttaaa cggccctctg tccttgagta
     5461 cgggatcccg acgaagcgtg ccggcaacag cgtcgggatc ggcatctaga tccagcaatc
     5521 tgcgcgagcg gtcgactgcc ga
//
LOCUS       DATMED010000314         5467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2572041, whole genome shotgun sequence.
ACCESSION   DATMED010000314 DATMED010000000
VERSION     DATMED010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572041"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /gene="secE"
                     /locus_tag="VNP73_08040"
     CDS             complement(<1..269)
                     /gene="secE"
                     /locus_tag="VNP73_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWL65909.1"
                     /translation="MNRQTKRMMKKDEERKRTAPRPVKAAGSPTKERTKPTQFVREVV
                     AELQKVAWPSRQEVFAYSLVVLVSSVVIAALIFGMDFVFTKAVLAL"
     gene            complement(393..1598)
                     /locus_tag="VNP73_08045"
     CDS             complement(393..1598)
                     /locus_tag="VNP73_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65910.1"
                     /translation="MKRRTSGAVAFVVALGLSLAGPTSGGTATCRRPEQHEVHTTPKK
                     HRYLCKFKVVDSQIVSKTGGLGSIAVHGKVGAVLQRDEGIVAMLDLEDPTHPRTLGTY
                     SDDAQQSFDGDLAFSHDGQFLFYARQTHQFSRDGVHVLDVSDPTQPALAFYQASGGTL
                     RIAYYFDGTNEYVIFLDAVDGLVVTRFERQSGALVELFRDPGEPASKVGGPASAGLVV
                     DPKDPITGSPLLYVATGGDGLEIYDLTDPTVPVEVGSWPDAGLAEIEVVRTATKRTIY
                     AAPEYWFDKAPEPEVFVLDATDLGSIEKKRSYKLGLPTDQTWRVQGMARAGSRLLVAH
                     SHAGLVAFTRRGSIGARALMGAKPHLGAGEPVAGAPAVYAMDVEKRGKIFYLSDAATG
                     ALTVLRLVP"
     gene            complement(1595..2449)
                     /locus_tag="VNP73_08050"
     CDS             complement(1595..2449)
                     /locus_tag="VNP73_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65911.1"
                     /translation="MWLVNADGTGAEKIAQDLDHGAWRSKWSPDGRFLLYEGVQGIHV
                     LEVETGEKWTSDDWYEWDPDWSPDGERIVFSRFTGKGTDLFAISPDGSDEERLTWTPR
                     KDERWPIWSPDGNFIAFVRPGRATAPSFWVVDVRDGSERVLAEKWTSGPSAWSPDGST
                     LAFLSQRSDLFTVKIDGTQLRRLARTKFSESGLDWSPDGTQIVVGGGSLRVFRADGSG
                     IEWTAQEGAEYPQWSPDGETIAYEKRGDVWVIGVNAQGHRNITRTNKADEDWPSWSPD
                     GSRIAYMK"
     gene            complement(2593..2666)
                     /locus_tag="VNP73_08055"
     tRNA            complement(2593..2666)
                     /locus_tag="VNP73_08055"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2630..2632),aa:Trp,seq:cca)
     gene            complement(2681..3034)
                     /locus_tag="VNP73_08060"
     CDS             complement(2681..3034)
                     /locus_tag="VNP73_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWL65912.1"
                     /translation="MADIRCEEVREMLPAYAEDELTFPVRRHLGSCGDCRAELVRYEE
                     IASGLSALATVQLNPPVGLLPALRSIPEESGRVDDLVGHFLRNRAAYLGGAAVVAAGA
                     AGAALWRSRRRLATA"
     gene            complement(3027..3620)
                     /locus_tag="VNP73_08065"
     CDS             complement(3027..3620)
                     /locus_tag="VNP73_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWL65913.1"
                     /translation="MKRLKDARDRPPPQGIPPELIERCKQGDERAWNELVGATYRDVY
                     TLCLRILRDPNDAAEATQEAYVKVWKGLKAFRGDAALETWMYRVASNAAISKHRSRRR
                     RQAHEAGVGDGMLTEMAASGSVEEAAGAKVEVQALDKALRSLPEHYRSAVVLRDVYGF
                     STEEIAKQLKISETAAKVRVHRGRKKLKEMLYGSNDG"
     gene            complement(3668..4117)
                     /locus_tag="VNP73_08070"
     CDS             complement(3668..4117)
                     /locus_tag="VNP73_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL65914.1"
                     /translation="MARILIAEDNAEIRALVSSILAEEGHKVSVAQNGQQALDMMGED
                     APDLLVLDIMMPQVDGYGVLKELRNTGLRDTVKILVLTAKTSESDWVRGYKLGADSYI
                     TKPFDIDELTNQVNELLSMTKDQLRLRREQELDKAQLLSRLESIFDT"
     gene            complement(4138..4602)
                     /locus_tag="VNP73_08075"
     CDS             complement(4138..4602)
                     /locus_tag="VNP73_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL65915.1"
                     /translation="MANIHISDDEPDMRHLVAFLVAEMGHEVTVSIDGRDAIEAMLVR
                     PPDLLILDLMMPDMDGYAVLGEMHEYQILDSTRVLVLSARSSEDDRQLAFELGADEYV
                     TKPWESGELKAVITLLLERSKEELRLGREDLKDKARLLAQLETIIEQGQRTA"
     gene            complement(4681..4956)
                     /locus_tag="VNP73_08080"
     CDS             complement(4681..4956)
                     /locus_tag="VNP73_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWL65916.1"
                     /translation="MSVKVRIPTQLRTLTEGAGEVEASGDTLAEILEDLESRHPGMKE
                     RLLDDSGGLRRFVNVYLNDEDVRFLDGLSTSVPQGAQVSIIPAVAGG"
     gene            complement(4953..>5467)
                     /locus_tag="VNP73_08085"
     CDS             complement(4953..>5467)
                     /locus_tag="VNP73_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWL65917.1"
                     /translation="LTKIKKGFAEFNKVGLIDEEPRAVLHGAQAEGCSPIATAFASGD
                     DEVSPVKPDTVAKSLAIGNPADGYYALKDIRGSGGQAVSVPESDVAEGIRLLARTEGI
                     FTETAGGVTVKALETLVARGHIKPDQETVALITGIGLKTLEALGDARKTERIRPRVED
                     VDELLEEGSG"
BASE COUNT          997 a         1805 c         1565 g         1100 t
ORIGIN      
        1 agggcgagca cggccttggt gaatacgaag tccatgccga agatcagtgc agcgataacg
       61 accgagctga cgagaaccac gagtgaatag gcgaacacct cttgccggga aggccacgcg
      121 accttctgaa gctcggcgac cacttcacgc acgaattgtg ttggtttcgt ccgctccttg
      181 gttggtgaac cggcggcctt gaccggacgc ggagcagtac gtttacgctc ctcgtccttc
      241 ttcatcatgc gtttggtttg cctattcacg gcagcccttt ccgctgctgc aggtcggacg
      301 cgctcgtaag ggtcctaact gcggggggat agaaaccaag attagcacgg tcgtgcctgg
      361 gggtcgccgg gccgatcccc gacgaccgaa cgctaaggga ccaggcgaag aaccgtgagt
      421 gccccggtcg ccgcgtccga aagatagaag atcttgcccc gcttctccac gtccatcgcg
      481 tacacggcgg gagcccccgc caccggctca ccggccccca gatggggctt cgcccccatc
      541 agtgcgcgag caccgatcga accgcggcgc gtgaatgcga ccaagcccgc gtgggagtgt
      601 gcgaccagca gccggctgcc ggccctcgcc atcccctgca ccctccaggt ctggtcggtc
      661 ggcagtccca gcttgtagct ccgcttcttc tctatcgaac cgagatcggt cgcatccaag
      721 acgaacacct cgggctccgg agccttgtcg aaccagtact cgggggccgc gtagatggtc
      781 cgcttggtcg cagtccgaac gacctcgatc tcggccagcc cggcgtcggg ccacgagccg
      841 acctcgaccg gcaccgtcgg atcggtcaga tcgtagatct cgagaccgtc gccccccgtt
      901 gccacgtaca gcaacgggct tcccgtgatg gggtccttcg gatccacgac cagacctgcc
      961 gaagccgggc ccccgacctt cgaagcaggt tcacccggat cccggaacag ctcgacgagg
     1021 gcacccgact gtctctcgaa ccgcgtcacg accaacccgt ccaccgcatc cagaaagatc
     1081 acgtactcgt tcgtgccgtc gaagtaatag gcgatgcgga gggttccccc ggaggcttga
     1141 tagaaggcca gcgccggctg cgtcgggtcc gacacgtcaa ggacgtgcac cccgtccctc
     1201 gagaactggt gcgtctgtcg tgcatagaac aggaactgcc cgtcgtgcga gaaggcgagg
     1261 tcgccgtcga aggactgctg cgcgtcgtcc gaatatgtcc caagagtcct cgggtgggtg
     1321 ggatcctcca gatcgagcat cgcgacgatc ccctcgtcac gttgtaggac tgctccgacc
     1381 ttcccatgca ccgcgataga tccaagcccg ccggtcttcg agacgatttg agaatccacg
     1441 accttgaact tgcagagata gcgatgcttc ttgggagtgg tgtggacctc gtgttgctcg
     1501 ggccgtctgc atgtggcggt cccaccggaa gtcggccctg ccagtgacag cccgagggcg
     1561 acaacaaacg cgacggcccc gctcgttctg cgtttcattt catatacgcg atccgggaac
     1621 catctggcga ccacgagggc cagtcctcat cggctttgtt cgttctggtg atgttgcggt
     1681 ggccttgtgc gttgacgccg atgacccaga cgtctcccct cttctcatac gcgatcgttt
     1741 ctccatccgg cgaccactgc ggatactccg caccctcctg agcagtccac tcgataccgg
     1801 atccatcggc acggaacacg cggagacttc ctcctccgac cacgatttga gtcccgtccg
     1861 gggaccaatc gagtccggat tccgaaaact tcgttcgcgc cagcctcctg agctgtgttc
     1921 catcgatctt gaccgtgaag agatcagatc tctggctgag aaaggccagc gtcgagccgt
     1981 ccggcgacca tgccgaagga ccggaggtcc acttttcggc gagcaccctt tccgacccgt
     2041 ctctcacgtc gacgacccaa aacgatggtg ccgtcgctcg accgggtcgg acaaacgcga
     2101 tgaagtttcc gtccggcgac caaatcggcc agcgctcatc tttcctgggt gtccacgtca
     2161 acctttcttc atcggaacca tccggggata tagcgaaaag gtcggtgcct tttccggtaa
     2221 atctcgagaa cacgatccgt tctccatccg gcgaccagtc cgggtcccac tcgtaccagt
     2281 cgtccgacgt ccacttttca cctgtctcca cttccagtac gtggataccc tgcacaccct
     2341 cgtagagaag aaatctgccg tccggtgacc atttggatcg ccaagcccca tgatcaaggt
     2401 cctgggcgat cttctcggcg cccgtgccat cggcgttcac aagccacatc ttcggggggt
     2461 agatcggtgg gctcgcgtat acggaaggcg tcgaggtcaa aaaagccacc acgaggatgc
     2521 ccgcggctag caacgatctg cgcactcgtc caccccgtcg cacggccttc tcccccgaga
     2581 acgtcctgtc agcagggcgg gagggactcg aacccccggc ctacggtttt ggagaccgtt
     2641 gctctaccaa gctgagctac cgccctaggg tcgaacgagt ctaggccgtt gcgagcctgc
     2701 gacgagaacg ccacagcgct gctcccgcgg cccccgcggc gactaccgcc gctcccccga
     2761 ggtaggcggc gcggttgcgc aggaagtgac cgacgagatc gtccacgcga ccgctctctt
     2821 ccgggatcga gcgcaatgcc ggcagcaagc cgacaggtgg gttgagctgg accgttgcaa
     2881 gtgcgctgag gccggatgcg atctcctcgt atcggacgag ttcggcacga cagtctccgc
     2941 aggacccgag atgtcgtctc accggaaagg tcaactcatc ctcggcatag gcaggaagca
     3001 tctcgcgcac ctcttcgcac cggatgtcag ccatcgttcg acccatagag catctctttc
     3061 aacttcttcc gtcctcggtg gacccgtacc ttcgctgcag tctcggagat cttgagttgt
     3121 ttggcgatct cctcggttga gaagccatag acatctcgta gaacgacggc cgaccggtaa
     3181 tgctccggca acgacctgag cgccttatcg agagcctgga cctcgacctt ggcacccgcc
     3241 gcctcttcaa ccgatcccga agcggccatc tctgtgagca tcccatcgcc tacccctgct
     3301 tcatgcgctt gcctgcgcct acggctccgg tgcttggaga tcgccgcgtt agatgcaacg
     3361 cgatacatcc aggtctccag tgcagcgtcg ccgcggaacg ccttcagccc tttccaaacc
     3421 ttgacgtagg cctcctgcgt cgcctcggcg gcgtcgttgg ggtcccggag gatcctcaag
     3481 cacagcgtgt agacgtctcg gtaggtcgcc cccacaagct cattccacgc tctctcatcg
     3541 ccctgcttac aacgctcgat gagttcgggg ggaatcccct gtgggggtgg ccggtcccga
     3601 gcgtccttaa gacgcttcat ctagataaag gttactgggg actaacgctc gttcctagga
     3661 gggagatcta ggtatcgaag atggattcga ggcgggacaa gagctgtgct ttgtcgagct
     3721 cctgctctcg tcggaggcgg agctggtcct tagtcatgga tagcaactcg ttgacctggt
     3781 tcgtcagttc atcgatgtcg aacggtttcg tgatgtagga gtcggcacca agcttgtatc
     3841 ccctgaccca gtccgattcg gacgtcttgg ccgtcagaac gaggatcttc accgtgtccc
     3901 gcaggcccgt gttccgaagt tctttgagaa cgccgtatcc gtcgacctgg ggcatcatga
     3961 tgtcgaggac gaggagatcg ggcgcgtcct ctcccatcat gtcgagcgcc tgctgcccgt
     4021 tctgggcgac gctgaccttg tggccctctt ccgcgaggat cgaactgacg agcgctctga
     4081 tctcagcgtt gtcctcggcg atgaggatgc gcgccaatag tcctcctccc gaccgattca
     4141 agcggtccgc tgcccctgct cgataatcgt ctcgagctga gccagcaatc ttgccttgtc
     4201 tttgaggtct tcgcgcccca gcctaagttc ttctttgctc cgttccaaca gcaaagtgat
     4261 aaccgccttc agctctcccg attcccaggg tttggttacg tactcgtccg cccccagctc
     4321 gaaggccaat tgccggtcgt cttcgctgct ccgtgcggac agcaccagga ccctggtcga
     4381 gtcgaggatc tggtactcgt gcatctcacc aagtaccgca tatccgtcca tatcgggcat
     4441 catcagatcg aggatcaaaa gatcgggcgg cctcaccagc atggcttcga tggcatcgcg
     4501 gccgtctatc gaaacggtca cctcatgacc catctctgca accaggaaag caacgagatg
     4561 acgcatatcg ggttcgtcat cggagatgtg gatgttcgcc actgcggggg cctcctatgg
     4621 gggctcggcg gttccctaag aatcggtcgc gccgcccgcg tccttaagcc ctaggagcga
     4681 tcaacctccg gcgacggcgg ggatgatcga gacctgagcc ccctgaggca cggaggtcga
     4741 taacccgtcg aggaaccgga cgtcctcgtc gttcagatac acgttcacga atctacgcaa
     4801 gcctccggaa tcgtccagga gacgctcctt catcccggga tgacgagact cgaggtcttc
     4861 gaggatctcg gccaacgtgt caccgctcgc ctcgacctcg ccggcccctt ccgtaagggt
     4921 tcggagttgc gtcgggatcc tcactttcac gctcatccgg aaccttcctc cagaagctcg
     4981 tcgacatcct cgacccgcgg gcgtatccgc tccgttttcc tcgcgtcgcc caaagcttcg
     5041 agcgtcttta acccgatacc ggtgatgaga gccaccgtct cttgatcggg cttgatgtgc
     5101 ccgcgagcca cgagtgtttc cagagctttc acggtcactc cgcccgccgt ctcggtgaag
     5161 atcccctcgg tgcgcgcgag aaggcggatg ccttcggcga cgtcgctctc cggcactgaa
     5221 acggcttggc cgccggatcc acggatgtcc ttcagagcgt agtaaccgtc ggcggggttg
     5281 cctatggcaa gcgacttcgc cacggtgtcc ggcttgaccg gcgagacctc atcgtcgccg
     5341 gaggcgaacg cggtcgcgat cggagagcat ccttcggctt gagctccgtg cagcaccgcc
     5401 cgaggttctt catctatcag gcccaccttg ttgaactcgg caaacccctt cttgatcttc
     5461 gtcagaa
//
LOCUS       DATMED010000315         8148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3342758, whole genome shotgun sequence.
ACCESSION   DATMED010000315 DATMED010000000
VERSION     DATMED010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342758"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1112)
                     /gene="hppD"
                     /locus_tag="VNP73_08090"
     CDS             complement(18..1112)
                     /gene="hppD"
                     /locus_tag="VNP73_08090"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HWL65918.1"
                     /translation="MTEREDAFPILGIDFIHFYVGNAKQAAHYYSAFGFEPIAYRGLE
                     TGTRDRVSWCVRQGDIRFVFTGALDPESPIADHVRLHGDGVHDVALSVPDAEEAYRIA
                     VERGARSVLEPQVDEDEHGKVVRAAIATYGQTIHSLVSRQEYSGVFWPGYQAVENGTV
                     EGVGLLAIDHVVGNVELGKMNEWVSFYERVMGFTELRHFSDEEISTEYSALMSKVVWD
                     GEGKIKLPINEPAEGRNKSQIEEYLEYFKTPGVQHIAMTSSDIVSTVEAMRAAGIEFL
                     RVPGTYYEDVKQRVPEIAEQIDGLQRNGILADKDEDGYLLQIFTKPVQDRPTVFFEVI
                     ERHGAKGFGAGNFKALFESIEREQAARGNL"
     gene            complement(1172..1468)
                     /locus_tag="VNP73_08095"
     CDS             complement(1172..1468)
                     /locus_tag="VNP73_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65919.1"
                     /translation="MTEWKAFRRLCTEALGIERTPAQLGDAWTRLEQTTLLPLSSDPG
                     HNEFRAAAIAARLMTRAATLRLESRGVHHRVDHPDPVPAWAGVRLRLARNQFHP"
     gene            complement(1494..2684)
                     /locus_tag="VNP73_08100"
     CDS             complement(1494..2684)
                     /locus_tag="VNP73_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWL65920.1"
                     /translation="MSDRIETDVLIVGAGAAGLFCSLHLPDQLEILVVDKGGRGRGSS
                     PWAQGGVAAALGPEDSPELHAQDTSRVAAGHADESAVAVLCEEAPECVLELAELGCAF
                     DRSEDGSLHLAREGGQSVPRSAHAGDATGAAIMDTLRSRAGPRVDRIEATCIGLAVAD
                     GRCIGGWLLVGDRVVEVRATATVLATGGLGGLFETTTNPAGATGEGIVLAWDAGAGLA
                     DLEFVQFHPTALVAGEGPQRPLITEALRGAGAHVVDAEGRRFLFEHHSDGELAPRDVV
                     ARAIASRRSWLDCRHIPRDVFEHEFPTVLKACLAEGLDPATDRLPVAPAAHYSIGGVQ
                     TDLDGRSSIPALCDRGMLEHGGPRCQPSRRQLSCRSARFRPACGTRDRGSADGSRQGL
                     PDTS"
     gene            complement(2681..3466)
                     /locus_tag="VNP73_08105"
     CDS             complement(2681..3466)
                     /locus_tag="VNP73_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWL65921.1"
                     /translation="MSVSVERFDGVTALTLDRPDVLNAFNDELGAEFLESVEKAAADD
                     DVRCIVLTGSGRAFSSGEDLGALADTYEKGDVPGIGDTLVRRYNPLIRAIRGAEKPVI
                     AAVNGVAAGAGASIALACDYRIASDKAKLVLAFIKVGLVPDAGGLWFLAKMIGTSRAW
                     ELASSGRPVAADEAKDLGLFHQVVPIDEFEGAWRSFATDVAAGPTRAFALTKKLLETA
                     TEVGLDAQLDAEVDAQSAAGSTADHLEGVQAFLQKRPPKFQGR"
     gene            complement(3520..5112)
                     /locus_tag="VNP73_08110"
     CDS             complement(3520..5112)
                     /locus_tag="VNP73_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65922.1"
                     /translation="MAVGEELEDQFRADRVARSAGISRSKLTLSEVDKRRTQLWGITI
                     FLFVAVTLGLALATVGSEFLPQELRINRLVDLSSWVVAVLVGGLALAFLIYVIEKEIS
                     LRRLTSLLIDERVLSAALSNRITEISRLSEMGKAINTTLELTDVLQMILFSALDLLEG
                     DEGSIMLLNEEQQLEVVASQGRSMENWTREVGTGISGEVAQTLKPKLIQGVHEKGNKK
                     RQINSAMSVPLVRLDELVGILNVNETQKRRRFTDQDLNALGFFAEHAAVAIGNARTYE
                     QERKAVERFEELDQLKSDFVATVSHELKTPLTAIIGSAQTLNRRRDRMSEEQQATLVG
                     MIERQGNRLLRLVEDVLTTSRIESRIAKLRRESIDLRQISEIAVEDLLHTEVGAGREM
                     IVKSEPERPTVWGDLGAIQQILANLIENALKYSDEGTPVTILVQEKDKEATIEVADEG
                     VGMTEEQIQTIFDRFHQLDSSSTRDRGGIGLGLYIVKSLIEAMNGTIEVESRPGEGSS
                     FRVRLPKRARDWPEELRTNTAG"
     gene            complement(5091..6548)
                     /gene="purF"
                     /locus_tag="VNP73_08115"
     CDS             complement(5091..6548)
                     /gene="purF"
                     /locus_tag="VNP73_08115"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWL65923.1"
                     /translation="MSGEARDLCGVTGIFAPGEDVARLTYYALYALQHRGQESAGIAI
                     SEGKTIVVYKELGLVAQALDETVLKSLQGYVAIGHTRYSTTGATSWENAQPSYKSSSK
                     GQIALAHNGNLVNSVDLKKTLDEAGGDPTPQAARLPSSSDTDLVAAHIARANEGDMKE
                     AILKVLPKLVGAYSFTMMDESRVFAARDPQGFRPLCIGRLDSGGWVVVSETCALDLLG
                     AKFIRDVEPGELITIDENGLDSQKILPTQNSACVFEHVYFARPDSNLMGQNVYATRYR
                     MGEQLASEAPCVAELVMPVPDSGVPAAQGFAHASNISYGDGFVKNRYVGRTFIQPTQS
                     MRQQGIRMKLNPVREVIAGRRVVVIDDSIVRGNTTRQIVAMLREAGAREVHMRISSPP
                     IKWPCFYGIDMPDQDELIGAQMSVEEIRGHIGADSLAYLSMEGMLQATGIPADNFCTA
                     CFSSRYPVSIPSEELRRKNVLEEPTADKTWQSEKN"
     gene            complement(6561..>8148)
                     /gene="purL"
                     /locus_tag="VNP73_08120"
     CDS             complement(6561..>8148)
                     /gene="purL"
                     /locus_tag="VNP73_08120"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWL65924.1"
                     /translation="TGRDGIGGASVLASAEFDEGSEDKRPSVQVGDPFTEKLLIEACL
                     ELIRRDLLVGFQDLGAGGISCPTSEMSAKAGTGMRVNVDSVHRREPGMEPFEVMISES
                     QERMLGVVAPPDLDAVLEVCNRWGLEARVIGEVVDSPRLEISSGGEVVADVPARALAE
                     EGPEYHRPVDRPAWIDAIQSAPCGDDPPTDLHAALMAICASPTVASKRWVWEQYDHMI
                     FLGTVQGPGGDAAVVRLPDSEVAVALSVDGPGRFCQLDPYEGARLAVAEAARNVACVG
                     AHPLAVTNCLNFGNPEKPEVMWQFSEVVRGIGDACRELGTPVTGGNVSFYNETKGRAI
                     YPTPVIGMLGVVKKPARALGIGFRRPGDDIVLLGTGDPSDLGGSEYAKVVNGVVAGRP
                     PRLDLQMELKLHKLLFESARDGILVSAHDLSEGGLAIALLESAMANGMGFKVDVSSEN
                     AYRDLFCESPTRVLVSCDPDARDRLIDMAETLGVPARLIGVVDGSEMTFGSALTVDPA
                     AVKDAYETALERSLMGGSDK"
BASE COUNT         1494 a         2691 c         2439 g         1524 t
ORIGIN      
        1 agagaaaacg acgtccatca gagattcccg cgagctgctt gttcgcgctc gatcgattcg
       61 aacagggcct tgaaattgcc ggcgccgaaa cctttcgcac cgtggcgttc gatcacctcg
      121 aagaacacag tcgggcgatc ctgcaccggc ttcgtgaaga tctgcaggag gtaaccgtct
      181 tcgtccttgt cggccaagat cccgttgcgc tgcaggccgt cgatctgctc ggcgatctcg
      241 ggcaccctct gtttgacgtc ttcgtagtag gtgccgggca cgcgcaggaa ctcgatcccg
      301 gcagcgcgca tcgcttcgac ggttgacacg atgtcgctgc tcgtcatcgc gatgtgctgc
      361 acgccgggtg tcttgaagta ctcgaggtac tcctcgatct gagacttgtt gcggccctcg
      421 gcgggttcgt tgatcggcag cttgatcttg ccttctccgt cccacacgac cttcgacatc
      481 agcgccgagt actccgtcga gatctcttca tccgagaagt gcctgagctc cgtaaagccc
      541 atcacacgct cgtagaaaga cacccattcg ttcatcttgc ccagctcgac attgccgacg
      601 acgtgatcga tcgcgagaag gccgactccc tcgaccgtgc cgttttccac ggcctgatat
      661 ccgggccaga agacacccga gtactcctgc cggcttacca gcgaatggat cgtctggccg
      721 taggtcgcta tcgccgcccg cacgactttg ccgtgctcgt cctcgtcgac ctggggctcg
      781 agcacactgc gagcgccgcg ttcgacggcg atccggtacg cctcttcggc gtccgggacg
      841 ctcaaagcaa cgtcgtgaac gccgtctcca tgcagccgta catggtcggc tatgggactc
      901 tcgggatcga gggcgccggt gaacacgaag cggatgtctc cctgccgaac gcaccaggag
      961 acccgatcgc gggtcccggt ctcgagacct ctgtaggcga tgggctcgaa gccgaaagcg
     1021 gagtagtagt gcgccgcttg tttggcattg cccacgtaga aatggatgaa gtcgatgccg
     1081 agtatcggaa acgcgtcttc gcgctcagtc atagcgagta gccacctcct tggagccgcc
     1141 ccaaggataa ggctcgggtg agcggggtgt cctatggatg gaactgatta cgggctagac
     1201 ggagccgcac tccggcccac gcggggaccg gatcgggatg atctacgcgg tgatgaaccc
     1261 ctctggattc gagccgcaga gtggcggctc gcgtcatcaa gcgcgccgcg atggccgccg
     1321 ctctgaactc gttgtgtcca ggatcggatg acagcggaag cagcgtcgtc tgttcgaggc
     1381 gcgtccaagc atccccaagc tgtgcggggg tccgctcgat ccccagcgcc tccgtacaca
     1441 gtctccggaa agctttccac tccgtcacgg tgatcgggat gccgggcctt acctcaggag
     1501 gtgtcgggaa gcccttgacg cgacccgtcc gctgacccgc gatcgcgcgt gccgcacgcc
     1561 ggccgaaaac gagcgcttcg gcaagagagt tgccggcgag acggttggca ccgtgggccc
     1621 ccgtgttcga gcattccccg atcgcataga gccgggatcg agctgcgccc atcgaggtcc
     1681 gtctgcaccc cgccgatcga ataatgcgcg gccggcgcga ccggaagccg atcggtcgcc
     1741 ggatcgagcc cttcggcgag gcaggccttc aataccgttg ggaactcgtg ttcgaagaca
     1801 tctctcggga tgtggcggca atcgagccac gacctgcgcg atgcgatcgc acgagcgaca
     1861 acgtcgcgag gagccagctc accgtcggag tgatgctcga acaggaaacg ccggccttcc
     1921 gcgtcgacaa catgggcgcc cgccccgcgc agcgcctcgg tgatcagcgg ccgctgggga
     1981 ccctctccgg ctaccagggc cgtgggatgg aattgcacga actcgaggtc ggccagcccc
     2041 gcccccgcgt cccacgccag cacgatgcct tcgcccgttg cacccgccgg gttcgtcgtc
     2101 gtctcgaaca agccgcccag cccacccgta gccagcacgg tggcagtggc tcttacctcg
     2161 acaactcggt caccgacgag cagccagccc ccgatgcaac gtccgtctgc cacggccaga
     2221 ccgatgcacg ttgcttcgat gcggtcgacg cgcgggcctg cccgactacg cagtgtgtcc
     2281 atgatcgcgg cgccggttgc gtcgccggca tgagccgaac gggggaccga ttgaccaccc
     2341 tcgcgtgcga ggtggagcga gccatcctcc gaacgatcga acgcgcaccc gagctcggcc
     2401 agctcgagca cgcactcggg tgcctcctca cacagcaccg cgactgccga ttcgtcggca
     2461 tgcccggcgg ccacccgact cgtgtcctgg gcgtgtagct cgggcgagtc ctcgggtccc
     2521 agcgcggccg cgacccctcc ttgagcccac ggggaggaac cccggccccg gccgcccttg
     2581 tcgacgacga ggatctcgag ttgatccggt aggtggagcg agcagaacag tcccgctgca
     2641 ccggcgccga ctatcaggac gtcggtttcg atgcggtcgc tcatcgacct tggaacttgg
     2701 gcggccgttt ctggaggaaa gcctggacgc cctcgaggtg atcggcggtc gatcccgcag
     2761 cactctgcgc gtcgacctct gcatcgagct gagcatccaa gcccacctcg gtcgcggtct
     2821 ccagcagctt cttggtcaag gcgaatgccc tcgtcggacc tgccgcaaca tccgtagcga
     2881 aggagcgcca cgctccttcg aactcgtcga tcggaacgac ctgatggaag agaccgagat
     2941 ccttcgcttc atcggcggcc acgggccggc cactggaagc gagctcccac gcacgcgagg
     3001 tgccgatcat cttggccagg aaccacagcc caccggcatc gggcacgagt ccgaccttga
     3061 tgaaggcgag aacgagcttc gccttgtccg aagcgatcct gtagtcacat gcgagggcga
     3121 tacttgcccc ggccccggct gcgactccgt tgaccgcagc gatcaccggc ttctcggctc
     3181 cccttatagc ccggatcaag gggttgtaac gccgcacgag cgtgtccccg ataccgggca
     3241 cgtcgccttt ctcgtaagtg tcggcgaggg cacccagatc ttcgccggag gagaacgccc
     3301 ggccggaacc cgtgagcacg atgcagcgca cgtcgtcgtc ggcggccgcc ttctcgacgg
     3361 actcgaggaa ctcggcgccc aactcgtcgt tgaacgcgtt cagaacgtcg ggacgatcga
     3421 gcgtgagagc ggtgacgccg tcgaatcgct cgaccgaaac ggacatgccc gcaggctagc
     3481 ggagcgggct acgcccgcgc aatcagtacc gcccacccac tacccagcag tgttagtgcg
     3541 gagttcctcc ggccagtctc gggctcgttt gggcagacgg acccggaaag aggatccttc
     3601 gcccggccgg ctctcgacct cgatcgttcc gttcatcgcc tcgatcaagc tctttacgat
     3661 gtagagacca agcccgatcc caccccgatc ccgggtgcta gacgagtcga gctgatggaa
     3721 acggtcgaag atcgtctgga tctgctcctc tgtcatgccg acgccttcgt cggctacttc
     3781 gatcgtcgct tccttgtcct tctcttgaac caagatcgtt accggggtcc cttcgtccga
     3841 gtacttgagc gcgttctcga tcaggttcgc aaggatctgt tggatcgccc ccagatcacc
     3901 ccacacggtg ggccgctcgg gctccgactt cacgatcatc tcgcgcccgg ctcccacttc
     3961 ggtatgcaaa aggtcttcaa cggcgatctc ggagatctgg cgcaggtcga tcgactcgcg
     4021 gcgcagcttc gcgatgcggg attcgatgcg cgacgtggtc aggacatcct caaccaatct
     4081 gagcagccgg ttcccctgcc gctcgatcat gccgacaagg gttgcttgtt gctcctcgga
     4141 catacggtca cgcctgcggt tcaacgtctg agccgaaccg atgatggccg tcaacggcgt
     4201 cttcagctcg tgcgaaacgg tagctacgaa gtcgctcttc agttgatcga gttcctcgaa
     4261 acgttcgacc gccttgcgtt cctgctcgta cgtacgggcg ttaccgatcg cgaccgccgc
     4321 gtgttcggcg aagaacccga gggcattcag atcctgatcg gtgaatcgcc tacgcttttg
     4381 ggtctcgttg acgttcagga tccccaccaa ctcgtctagt cggacgagcg gcacggacat
     4441 cgcgctgttg atctgacgtt tcttgtttcc cttctcgtga acaccctgga tcagtttcgg
     4501 cttgagagtc tgggcaacct ctcctgatat gcccgttccg acctcccgcg tccagttctc
     4561 catggagcgc ccttgcgagg cgactacctc gagttgttgc tcctcgttga gaagcatgat
     4621 cgagccctcg tcaccttcta gaaggtcgag agcggaaaaa aggatcatct gcaagacgtc
     4681 ggtcaactcg agcgttgtgt tgatcgcctt gcccatctcc gacaaccgcg agatctctgt
     4741 gatccggttg gatagagctg ccgagagaac gcgctcgtcg atgaggagcg aagtgagccg
     4801 gcgcagactg atctccttct cgatgacgta gatcaagaaa gcgagtgcaa gcccaccgac
     4861 cagcaccgcg acgacccatg acgagagatc gacgagccgg ttgatcctca gttcctgcgg
     4921 caagaactcg gaaccgacgg ttgcgagcgc gagacccaag gtcacggcca cgaacaagaa
     4981 gatcgtgatg ccccacagct gggtccttcg cttatcgacc tcggaaagcg tcagcttgga
     5041 ccggctgatg cccgcactgc gggcaacgcg atccgcgcgg aactgatctt ctagttcttc
     5101 tccgactgcc atgtcttgtc ggccgttggc tcctcgagaa cgtttttccg tcgcagttct
     5161 tcgcttggga ttgatacggg atatcgagaa gaaaagcatg ccgtacagaa gttgtcggcc
     5221 ggaatgcccg tcgcctgcag catcccttcc atcgaaaggt aagcaagcga gtcggcgccg
     5281 atgtgcccac ggatctcctc aaccgacatc tgggcgccta tgagctcgtc ttggtcgggc
     5341 atgtcgatcc catagaaaca aggccatttg atggggggag acgagatccg catgtggacc
     5401 tctctagctc ctgcctcccg gagcatcgcc acgatctgac gggtcgtatt gcctcgcacg
     5461 atcgagtcat cgatcacgac cactcggcga ccggcgatca cctcccgcac cggattcaac
     5521 ttcatgcgga tgccctgctg gcgcatcgac tgggtgggtt ggatgaacgt ccggcccacg
     5581 tagcggttct tcacgaagcc gtcgccgtag gagatgttcg aggcgtgcgc gaagccttgg
     5641 gctgcgggca ccccgctgtc cgggacgggc atcaccagtt ccgcaacaca tggggcctca
     5701 ctcgcaagtt gctcgcccat cctgtagcgc gtggcataga cgttctggcc catgaggttc
     5761 gagtcgggcc gagcgaaata gacatgctcg aagacgcagg ccgagttctg ggtcggcaaa
     5821 atcttctggc tatcgagccc gttctcgtcg atcgtgatca gttcgcccgg ttcgacgtcg
     5881 cggatgaatt tggcccccag caggtccaac gcgcaggtct cggacaccac gacccacccg
     5941 cccgaatcga gccgcccgat gcacaaaggt ctgaagcctt gaggatcgcg cgcggcgaag
     6001 acacgcgact cgtccatcat cgtgaacgaa taggcgccca cgagcttcgg gagaaccttg
     6061 aggatcgcct ctttcatgtc gccctcgttc gcccgtgcga tgtgagctgc gaccagatcg
     6121 gtgtcggagg acgacggcag ccgcgccgct tgtggggtcg ggtccccgcc ggcttcgtcc
     6181 aacgtcttct tcaggtcgac ggagttcacg aggttcccgt tgtgtgccag tgcgatctgc
     6241 cccttcgacg agctcttgta gctgggctgc gcgttctccc aactggtcgc gccggtcgtc
     6301 gaataccgcg tgtgaccgat cgcaacgtag ccttgaaggc tcttcagaac cgtttcgtcg
     6361 agagcctgag cgacgaggcc cagctccttg taaacgacga tcgtcttacc ctcagagatc
     6421 gcgatgccgg ccgattcttg tccacgatgc tgaagggcat aaagcgcgta gtaggtcagg
     6481 cgcgcgacgt cctcgcccgg ggcgaagatc ccggttacgc cgcagagatc gcgggcctca
     6541 ccgctcattt gtcctcccgc tcatttgtcc gatcccccca tcaacgagcg ctccaatgcg
     6601 gtttcgtaag cgtctttcac tgcggccgga tcaacggtca gggcggatcc aaacgtcatc
     6661 tcggatccgt ccaccacacc gatcaagcgt gcggggacgc cgagggtctc ggccatgtcg
     6721 atcaacctat ctcgtgcatc gggatcacat gaaacgagca cgcgtgtcgg ggactcacag
     6781 aacaagtccc tgtacgcgtt ttctgacgaa acatccactt tgaagcccat cccgttcgcc
     6841 atcgccgatt cgagcagggc gatcgccagg ccgccttcgg acagatcgtg cgcggagacc
     6901 aagatcccgt ccctcgccga ttcgaacaac aacttgtgga gttttagttc catctgcaga
     6961 tccaaccgcg gagggcgacc ggcaaccacg ccgttaacga ccttggcgta ttcgcttcct
     7021 ccgaggtcgc ttggatcgcc ggtacccagc aacacgatgt cgtctcccgg ccggcggaag
     7081 ccgatcccga gcgctcgagc cggcttcttg accacaccca gcatcccgat cacgggtgtg
     7141 gggtagatcg cccggccttt ggtctcgttg tagaaggaga cgttcccacc ggtgacgggt
     7201 gtcccaagtt cccggcaagc gtcgcctatg ccgcggacca cttcagagaa ttgccacatg
     7261 acctcgggct tctccggatt gccgaagttg aggcagttcg taacggcaag aggatgcgcg
     7321 ccaacgcatg cgacgttgcg cgccgcctct gcaacggcga gccgggcgcc ctcgtaggga
     7381 tcgagctggc agaagcggcc cggcccatcg acactcaacg cgaccgccac ctctgaatcg
     7441 ggcagacgga caaccgcagc gtcgccaccc ggaccctgaa ccgtgccgag gaagatcatg
     7501 tggtcgtact gctcccatac ccaacgcttc gacgcgaccg tcggactcgc acagatcgcc
     7561 atcagggccg catgcagatc ggtcggggga tcgtcaccac aaggggccga ctggatggca
     7621 tcgatccacg ccggtcgatc gaccggccgg tgatactcgg ggccctcttc cgccagagca
     7681 cgggccggaa cgtccgcgac gacttctcca cccgaggaga tctcgagccg aggagaatcg
     7741 acaacctcgc cgatcacgcg ggcctcgagt ccccaccggt tacatacctc gagcaccgcg
     7801 tcgagatccg ggggtgccac aaccccaagc atgcgttcct gcgactcgga gatcatgacc
     7861 tcgaaaggct ccatgccggg ctcgcgccgg tgtacggaat ccacgttgac ccgcatcccg
     7921 gttccggctt tcgcactcat ctcggatgtc gggcacgaga tcccaccggc tccaaggtcc
     7981 tggaaaccga caaggaggtc ccggcggatc aattcgaggc acgcttcgat caagagcttc
     8041 tcggtgaacg ggtcgccgac ctgaacggat ggtcgcttgt cctcggaacc ctcgtcgaac
     8101 tcggctgaag ccaggacgct ggcaccaccg atcccgtcgc gtccggtg
//
LOCUS       DATMED010000316         8058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4861142, whole genome shotgun sequence.
ACCESSION   DATMED010000316 DATMED010000000
VERSION     DATMED010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4861142"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VNP73_08125"
     CDS             complement(<1..351)
                     /locus_tag="VNP73_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65925.1"
                     /translation="MKKISVVALLLLSLLAAACGGGPTTPAAGGGEEESSTPDAMEAE
                     AGTVAVTATEYAFEGVPAELPEGETTFTFENVGEEIHEMVVLRIKTDATAQELLKLPE
                     KEARKQVQTVGFAFA"
     gene            complement(421..993)
                     /locus_tag="VNP73_08130"
     CDS             complement(421..993)
                     /locus_tag="VNP73_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWL65926.1"
                     /translation="MPKTRTNVDREQKMTEILDLAAHKLRSGGYNAVSVAAIARELGI
                     AHNAIYWYFPSKDHLMVAAFEHIVQNVIARKPKGSGDVVKKVMWFVDQIADFYPLRAS
                     MHEQAQSSDVIATYLEDLNERLRRMTSHVLEPYVPSEELDLAVASFTATVQGACLAGI
                     QPSERRRVLGYALQRLIGGSRVARPRRKQA"
     gene            1101..1895
                     /locus_tag="VNP73_08135"
     CDS             1101..1895
                     /locus_tag="VNP73_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL65927.1"
                     /translation="MAWQRALITGSSAGIGEAFARELAAHGTDLVLVARREPLLKELA
                     VELERSHGVSVEVLPADLTDADQCRAVERRLSSPLEPIDLLVNNAGGGDPGAGAFVAH
                     DRDAVEKQAILNALSVLRLTHAALTSMKEQGRGNVIQVSAGVGFYPTPWGATYAASKA
                     FVNSFSQAVDFELGRGDVRVTSVCPGFTRTEAPARLGFSEDNVPRFLWTDPETVVAAA
                     LEGAASGKTLVSPTIVNQIASSVGMRVPKVMLKMAGRFRPLRESRS"
     gene            1892..2806
                     /locus_tag="VNP73_08140"
     CDS             1892..2806
                     /locus_tag="VNP73_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWL65928.1"
                     /translation="MKRALRVGVVLPTRETVIYGKLDPTPLLDMAEQAEHQGFDSVWV
                     GDSIFVSGRFEPLVLLSAIAARTRTVTLGTAILVAPLRHPVLLAHAVTSLDRISGGRV
                     VLGVGAGWLQQEFDAVGVPYAERTGRLAETIRCCRALWSGEGSFHGKYWSFDGVNLQP
                     LPQQEGGPPILVGGAGPRALRIAADFGDGWFPTSPDPAAFASGLAEVNPATAAVYLTI
                     NLDPERGPHEVRDYAQMYYGMPLDVMQQFQGYFVGPPDECLAWIRGYVDAGADHVVLR
                     FATPDAADQMSLAAEELLPELRGIEVEQ"
     gene            complement(2780..3592)
                     /locus_tag="VNP73_08145"
     CDS             complement(2780..3592)
                     /locus_tag="VNP73_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWL65929.1"
                     /translation="MKLQGNGVELEVGVQGEGPAVLLLHGFPDSSHLWRKQVPVLVEA
                     GFKVVAPDQRGFGASDKPEGVDNYNILNILEDTRSILDALGIDKAHVVGHDWGAAVAW
                     TFASLYQDRVASLVALSVGHPTAFGAASYEQLQRSWYMFMFQFEGVAEEWLSRNDFEF
                     LRSWIGGGGDIERYVEDLSRPGALTAGLNWYRANLPPQTWISEPLELPAISAPTMGIW
                     STGDDALTEDQMTDSQKYVQGPWRYERVEASHWIPLEAADRLNELLLDFYPS"
     gene            complement(3589..4713)
                     /locus_tag="VNP73_08150"
     CDS             complement(3589..4713)
                     /locus_tag="VNP73_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWL65930.1"
                     /translation="MYDVIVVGARCAGSPLAMQLARKGDRVLVVDRATFPSDTMSTHF
                     IQLPGMARLKRWGLLDRVLATGCPLVTHSITDEAGNKGEIEIPLVEGVPGLISPRRKI
                     LDTILVEAAAEAGAEIREGVSVDGLLFEGDRVVGIEGHTDAGAFSEKARFVIGADGKN
                     SVVAREVAAPVEHDFGKLTGGYYSYFSGLDCPSVELFIYPGAAVVMFPTHDGLTTVAF
                     VRPANTFKDMKRDIEGSFFSVMDQVGIGNRVRAATREEPYIGTGDVPNFIRKAHGPGW
                     ALAGDAAYHKDPTPADGISDAFRAADLLSGALDEILNGVDEEKALSSYQTAHDAIGLP
                     LLEKTAALSDTEGDPQERVQAFFEIRLMVHTEAEQVTAAA"
     gene            4803..5426
                     /locus_tag="VNP73_08155"
     CDS             4803..5426
                     /locus_tag="VNP73_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWL65931.1"
                     /translation="MPKVADPAVRDSLVETAARLIAEREPLSTRRLAAELGTSTMAVY
                     THFGSMDELRRAVRRDGFARLAEHMAKVHPTKDPVADLALAGHAYCVNALRNPHLYRV
                     MFMETPIDQEDAAVGLYTFEALVAIVQRCIDAGRFKSSGEPWSLAVQAWIAAHGVVTL
                     FLAGMMVEEQAIETLEAITKNMLVGFGDDPGSIARSFAAAEKREEVI"
     gene            complement(5423..5950)
                     /locus_tag="VNP73_08160"
     CDS             complement(5423..5950)
                     /locus_tag="VNP73_08160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL65932.1"
                     /translation="MRLIRRATGYDAGACAAIYKPIVEGSIISFEENAPEEAEMRERI
                     ESTLEEHEWLVYQEGDRILGYAYSGPFNKRAAYRWSIEISVYVSEDCRGRGIGRELTE
                     ALLEHASETGVVNAFAGVALPNPASVALFEKLGFERIALQEQVGYKLGAWHDVGWWQL
                     KLSTAPVPPPARSAG"
     gene            complement(5941..6792)
                     /locus_tag="VNP73_08165"
     CDS             complement(5941..6792)
                     /locus_tag="VNP73_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL65933.1"
                     /translation="MRAQRLVATAALVALVALACLMSPASAAERCFGRTATIVGTDRS
                     ETLRGTAGTDVIVGGSGGDRIFGRGGTDYICAGTGRDFVDDGDGDYEAVKGGRGTDEL
                     HATGDATIAFGGPDNDELNGGTGYVHLNGGGGDDVITGHGDEILHGNTGNDILRVTEG
                     RGDLFGEEGDDYLYGSEFDDELEGGAGDDILTGDAGNDILDGDAAPGHPATEEDGNDT
                     ILGGQGDDELRGQDGNDTLKGEEGDDSLAGDTGDDTLDGGDGIDTLDGGDGFDNCSGS
                     EQETACD"
     gene            complement(6792..>8058)
                     /locus_tag="VNP73_08170"
     CDS             complement(6792..>8058)
                     /locus_tag="VNP73_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL65934.1"
                     /translation="AAGELVQGGDIIDGGDGNDRMDGGKGIDSVDFGSGSGPVDASLE
                     RETATGNGNDKVANFEYLFGTGYDDVLTGNAEANDIGGRSGNDQIFGVEGDDELTGGN
                     GDDRFDGGEGIDTVDFTASPSGVTVDLGSGTSSGQGSDTFTTVEVLVGSSFDDTMEGD
                     DLPNVLIGGTGNDSLSGAGGNDDLQSNFGDDTLSGGDGDDILTDAGGADTSMGDAGDD
                     VFVGGTNDDRFDGGDGIDTVDFSLARRMIQADLEAGTSDGQGLDILVGVENLTGGIRD
                     DILAGDANVNVLAGGPGVDQLSGRDGDDTIIGSDGDDIMDGGGGVDTASYALALQPIT
                     ADLEASFATGEGSDSLLAIEGLVGSVLTDFLRGDDATNPIDGGDGNDVVEGRAGDDAL
                     DGGKGNDYADGGTGTDSCASAESELNCES"
BASE COUNT         1360 a         2618 c         2511 g         1569 t
ORIGIN      
        1 cgcgaacgcg aagcccaccg tctggacctg cttgcgagct tccttctcgg gcagcttcag
       61 gagttcttgc gcagtggcat cggtcttgat gcgaaggacc accatctcgt ggatctcttc
      121 gccgacattc tcgaaagtga aggtcgtctc gccttcggga agctccgcgg gaacaccctc
      181 gaacgcgtat tcggtcgcgg tgaccgcgac cgtgccggct tcggcctcca tggcatccgg
      241 ggtgctcgat tcttcttccc ctccacccgc ggcgggtgtc gtgggaccgc ccccgcacgc
      301 tgctgcaaga agcgaaagca gcagcaatgc gaccacactg atctttttca tgtgtcgacc
      361 ctcctaatca ctcgaagggt gtgacgctac ccgtcgcgta tgtgggtcga aaccagcagg
      421 tcaggcttgc ttgcggcgcg gtcgcgcgac ccgcgagcct ccgatcagtc gttgcaatgc
      481 gtacccgagt acgcgcctcc gttcgctggg ctgtatcccg gcgagacagg cgccctggac
      541 ggtcgcggtg aaggacgcaa ccgcgaggtc gagctcttct gaaggaacgt acggctcgag
      601 cacgtgtgac gtcatacgtc ttagccgctc attgagatcc tcgagatagg tcgcgattac
      661 gtcggagctc tgagcttgct cgtgcatcga ggcacgcaaa ggatagaagt cggcgatctg
      721 gtcgacgaac cacatgactt tcttcacgac gtcgccgctc ccctttggtt tgcgggcgat
      781 cacgttctgc acgatgtgct cgaacgccgc aaccatcaga tgatccttcg aagggaagta
      841 ccaatagatc gcgttgtgtg cgatcccgag ctcgcgcgct atcgccgcaa ccgataccgc
      901 gttgtacccc ccggaccgga gcttgtgcgc agcaagatcg aggatctcgg tcatcttctg
      961 ttctcggtcg acgttcgttc gcgtcttggg catgcccaat cttcctgcgc gggcgcctag
     1021 ccgtccgcgc gttgagttga caggctgtca agaccgtgga agtctcggtt gacaggctat
     1081 caagaggggt ggcggtgagc atggcatggc aacgggccct gattaccggg tcatcggcgg
     1141 ggataggtga ggcgttcgcc cgggagctgg cggcgcacgg cacagatctc gttcttgtcg
     1201 cccggcggga accgcttctc aaggagctcg ccgtcgagct cgagcggagc cacggcgtct
     1261 cagtcgaggt tctgccggca gatctgaccg atgcagatca gtgtcgagcc gtcgagcgcc
     1321 ggttgtcttc tccgctcgaa cccatcgatc tcctcgtcaa caacgcgggt gggggagacc
     1381 ccggcgcggg agcgttcgta gcccacgacc gcgacgcggt cgagaagcag gcgatcctca
     1441 atgcattgtc ggtgcttcgg ttgacgcatg cggccctcac atcgatgaag gaacaggggc
     1501 gcgggaacgt gatccaggtg tcggccggtg tgggctttta cccgacgccg tggggcgcga
     1561 cctatgctgc aagcaaggca ttcgtcaaca gcttcagcca agctgtcgac ttcgagctgg
     1621 gccgcggtga cgtccgggtg accagcgtgt gcccgggatt cacgcggacg gaagccccgg
     1681 cccgccttgg cttctccgaa gacaacgttc ctcgattcct gtggacggac cccgaaacgg
     1741 tggtagcggc ggctctcgaa ggcgcggcgt cgggaaaaac actggtgtct cccacgatcg
     1801 tgaaccagat cgcatcatcg gtcgggatgc gagtccccaa ggtgatgttg aagatggccg
     1861 ggaggttcag accattgcga gagtcgcgtt cgtgaagcga gcgttgcggg tcggagtcgt
     1921 ccttcccacg cgcgaaaccg tgatctacgg gaagctcgac cccaccccgc tcttggacat
     1981 ggccgaacaa gcagaacatc agggcttcga ttcggtctgg gtaggcgatt cgatcttcgt
     2041 ctctggtcgc ttcgagccac tggtcctgct gtcggctatc gccgcccgca cgcgcacggt
     2101 aactctcggc accgcgatcc tcgtcgcgcc gttgcggcat ccggtgcttc ttgcgcacgc
     2161 ggtcacgtcg ctcgatcgga tctccggcgg acgggtcgtg cttggggtgg gcgcggggtg
     2221 gctccagcag gagttcgacg cggtcggcgt tccatacgca gagcgcaccg gccggctcgc
     2281 agaaacgatc agatgctgcc gtgcattgtg gtcgggcgag ggatcgttcc atggcaagta
     2341 ctggagcttt gacggggtga accttcagcc tcttccacag caagaaggcg gacctcccat
     2401 cttggttggg ggagcggggc cgagagcgct gcgcatagcc gccgatttcg gcgacggttg
     2461 gttcccaacg agccccgatc cggccgcctt cgcgtcgggt ttagccgagg tgaacccagc
     2521 aacagcggcc gtctatctga cgatcaacct cgatcccgag cgcgggccac acgaagtgcg
     2581 cgattacgcc cagatgtatt acggcatgcc attggatgtg atgcagcagt tccagggtta
     2641 cttcgtcggt cctcccgacg agtgcctcgc atggatacgt ggctacgttg atgccggtgc
     2701 cgatcacgtc gttctgcgct tcgccacgcc ggacgcggcc gaccagatgt cgctcgcggc
     2761 cgaggaactg cttcccgagc tacgagggat agaagtcgag cagtagctca ttcagtcgat
     2821 cggccgcctc gagcgggatc caatgcgagg cttccacgcg ctcgtagcgc cacggaccct
     2881 gcacgtattt ctgcgagtcg gtcatctgat cttccgtcag agcgtcgtcg cccgtgctcc
     2941 agatccccat cgtcggggcc gagatggccg gtagttcgag cggttccgag atccacgtct
     3001 gcggcggcag gttggcgcga taccagttga ggccggccgt cagggcaccc ggtctcgaca
     3061 gatcctcgac gtaacgctcg atgtcgccgc cgcctccgat ccatgagcgc aggaactcga
     3121 agtcgttgcg cgacagccat tcctctgcca cgccctcgaa ctggaacatg aacatgtacc
     3181 aggagcgttg aagctgctcg tagcttgccg ctccgaacgc agtgggatgc ccgaccgaca
     3241 gagcaacgag gctcgcgacg cgatcttggt atagggaagc gaacgtccac gcgaccgcgg
     3301 ccccccaatc gtgaccgaca acgtgcgctt tatcgatgcc gagggcgtcg aggatcgagc
     3361 gcgtgtcctc caggatgttc aagatgttgt agttgtcgac gccttcaggc ttgtcggagg
     3421 caccgaaacc gcgctggtcc ggcgctacga ccttgaaacc ggcctcgacc aggaccggga
     3481 cctgcttgcg ccacagatgc gacgagtcgg ggaagccatg tagtaacaga accgccgggc
     3541 cctcgccctg gacgccgact tcgagttcga ctccgttgcc ttgcagcttc atgcggccgc
     3601 cgtgacttgc tcggcttccg tgtgcaccat caaccggatc tcgaagaagg cttggacgcg
     3661 ttcctgcggg tcgccttcgg tgtccgacag cgccgcggtc ttctccagca acggcaagcc
     3721 gatcgcatcg tgtgccgtct gataactact caaggctttc tcttcgtcca ctccgttcag
     3781 aatctcgtcg agggcaccgc taagaagatc cgcggcgcgg aacgcgtcgc tgatcccatc
     3841 ggcgggagtg gggtctttgt ggtaggcggc atcgccggcc agcgcccatc caggtccatg
     3901 ggccttgcgg atgaagttcg gcacgtcgcc cgtaccgatg tacggctctt cgcgtgtggc
     3961 ggccctgacc cggtttccga tgccgacctg atccatcacc gagaagaagc tgccctcgat
     4021 gtctcgtttc atgtctttga atgtgtttgc cggccgcacg aatgcgaccg tggtgagtcc
     4081 gtcatgggtc gggaacatga ccaccgctgc gcccggatag atgaacaact ccaccgaggg
     4141 gcagtccagg ccgctgaagt acgagtagta cccgcccgtg agcttgccga agtcgtgctc
     4201 gacgggagcg gcgacctcgc gagcaacaac ggagttcttc ccgtcggctc cgatcacgaa
     4261 ccgggccttt tccgagaagg cgcccgcgtc cgtatggccc tcgataccca cgacacggtc
     4321 gccttcgaac aacagaccat cgaccgatac accttcgcgg atctcggccc ccgcttcggc
     4381 cgccgcttcg accaggatcg tgtcgaggat cttccggcgg ggcgagatca atccggggac
     4441 accttctacg agtgggatct cgatctcgcc cttgttcccc gcctcatcgg tgatcgaatg
     4501 cgtgacgagt gggcatcccg tggcgaggac ccgatcgagc aggccccagc gcttgagtct
     4561 ggccatcccg gggagctgga tgaagtgtgt ggacatggtg tcgctgggaa acgtcgcacg
     4621 atcgacgacc agcacgcgat cgcctttgcg ggcgagttgc atagcgagcg gagaccctgc
     4681 gcagcgggct ccaaccacga tcacgtcgta catagcggcc ccatttctga agacaatttc
     4741 aaccaatgca taacagcgtt ggccttagat agataacacc gatatggcat actgtcaaaa
     4801 cgatgcctaa ggttgccgat ccggcggtca gagacagcct tgtcgagact gccgcccgct
     4861 tgatcgccga gcgcgaaccg ctcagtaccc gccgtttggc tgccgagctg gggacctcga
     4921 cgatggccgt ctacacccac ttcggcagca tggatgagct tcgccgggcc gtccgcaggg
     4981 atggctttgc tcgtcttgcc gagcacatgg cgaaggttca cccgaccaag gacccggtcg
     5041 ccgacctcgc tctggccgga cacgcttatt gcgtcaacgc gttgcggaat ccccatctgt
     5101 accgggtcat gttcatggag accccgatcg atcaggaaga cgccgcggtc gggctctaca
     5161 cgttcgaggc tctcgtggcg atagtgcagc ggtgcatcga cgccggtcgc ttcaagtcgt
     5221 cgggggagcc atggtcactc gcggtccaag cttggatcgc ggcccacggt gtcgtgacgc
     5281 tcttcctagc gggaatgatg gttgaggagc aggcgatcga aacactcgag gccatcacca
     5341 agaacatgct cgtcggcttc ggcgacgatc ccggttcgat cgcgcgttcg tttgccgcag
     5401 ccgaaaaacg cgaggaggtt atctagcccg cggatctagc cggagggggc accggggcgg
     5461 tcgataactt caactgccac cagccgacgt cgtgccacgc accgagtttg tagccgacct
     5521 gttcttggag agcgatccgt tcgaagccca gcttctcgaa cagagcgacg ctggccggat
     5581 tgggcagagc gacaccggcg aacgcgttga ccacaccggt ttcactcgca tgttcgagca
     5641 gagcctcggt gagctcgcgt ccgatccctc gtcccctgca gtcctcagag acgtacaccg
     5701 agatctcgat cgaccaccga tacgcggcgc gcttgttgaa tggcccgctg tacgcgtagc
     5761 cgaggatccg gtctccttct tgatagacga gccattcgtg ttcctcgagc gtggattcga
     5821 tgcgctcgcg catctcggct tcttctggtg cgttctcctc gaaagagatg atcgagccct
     5881 cgacgatcgg cttgtagatc gcggcacatg cacctgcgtc gtatccggtg gccctacgga
     5941 tcaatcgcac gcggtctctt gttcgctccc cgagcagttg tcgaagccgt ctccgccatc
     6001 gagcgtatcg atgccgtcac ccccgtcgag ggtgtcgtct cccgtgtcgc cggcaagtga
     6061 gtcgtcgccc tcttcacctt tgagggtgtc gttgccatcc tgtccccgca gttcgtcgtc
     6121 accctgccct ccgaggatcg tgtcgttccc gtcttcctcg gtcgcgggat ggccgggggc
     6181 cgcgtccccg tcgaggatgt cgttaccggc gtcaccggtg aggatgtcgt cgccggctcc
     6241 cccctcgagc tcgtcgtcga actcggaccc gtagaggtag tcgtcgccct cttcaccgaa
     6301 aaggtcgccg cgaccctcgg tcacccgcag gatgtcgttg ccggtgttgc cgtgcaggat
     6361 ctcgtcccca tgtccggtga tgacgtcgtc gccaccaccg ccgttcaggt ggacgtaacc
     6421 cgtgccaccg ttgagctcgt cgttgtcggg gccgccgaag gcgatggttg catcgcctgt
     6481 ggcgtgcagc tcgtcggtgc cccggcctcc cttcacggcc tcgtagtcgc cgtctccgtc
     6541 gtcgacgaag tcgcgccccg tcccggcaca gatgtagtcc gtgcctcctc gtccgaagat
     6601 gcggtcacca ccggagccgc cgacgatcac gtcggtgccg gcggtgccac gcaaggtctc
     6661 ggaccgatcg gttccgacga tggtggccgt tctaccgaag catcgctctg ccgccgaggc
     6721 cggcgacatc aggcaggcga gagcgacaag agcgacgagg gcagccgtgg ccaccagacg
     6781 ttgggctcgc atcagctttc gcagttcagt tcgctctcgg cgctggcaca ggagtcggtg
     6841 cccgtgcctc cgtcggcata gtcgttgccc ttgcccccgt ccagagcgtc gtcgccggca
     6901 cggccctcga ccacgtcgtt gccgtcaccg ccgtcgatgg gattcgtggc atcgtcgccg
     6961 cgcaggaagt ccgtaagcac ggagccgacc aatccttcga tcgccaggag gctgtccgaa
     7021 ccttcaccgg tggcaaagct ggcttccaga tcggcggtaa tcggttgcaa cgcgagcgca
     7081 taagacgccg tgtccacacc gccaccaccg tccatgatgt cgtcgccgtc gctaccgatg
     7141 atcgtgtcgt cgccgtcccg accagatagc tggtccacac ccggaccacc ggccaagacg
     7201 ttcacgttcg cgtcccctgc gaggatgtcg tcgcggatcc caccggtcag gttctcgacc
     7261 ccaacgagta tgtccagacc ttgtccgtcc gacgtaccgg cctcgaggtc ggcctggatc
     7321 atgcggcgcg cgagagagaa gtcgacggtg tcgatcccat cgccgccgtc gaagcgatcg
     7381 tcgttcgtcc caccgacgaa gacgtcgtcg cctgcatcgc ccatggaagt gtccgcgcca
     7441 ccggcgtcgg taaggatgtc gtcgccgtcc ccaccggaca gggtgtcgtc gccgaagttg
     7501 gattgaaggt cgtcgttacc gccggccccc gagagcgagt cgttcccggt gccgccgatc
     7561 agcacgttcg gtaggtcgtc gccctccatg gtgtcgtcga aagacgagcc gaccagcacc
     7621 tcgacggtgg tgaaggtgtc ggagccctgc ccactcgacg tgccgctgcc gagatcgaca
     7681 gtcacgccac tcggggaagc agtgaaatcg accgtgtcga taccttctcc gccgtcgaaa
     7741 cggtcgtcgc cgttaccacc cgtcagttcg tcgtcgccct cgactccgaa gatctggtcg
     7801 ttgccggagc gtccgccgat gtcgttcgct tctgcattgc cggtcagtac gtcgtcgtaa
     7861 cccgtaccga agaggtattc gaagttcgcc accttgtcgt tcccattgcc ggtcgccgtc
     7921 tcgcgctcga gggacgcgtc caccggcccc gatcccgatc cgaagtcgac cgagtcgatg
     7981 cctttgccgc cgtccatccg gtcgttgccg tcaccgccgt cgatgatgtc gccaccctgg
     8041 accagctcgc cggcggcc
//
LOCUS       DATMED010000317         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_644906, whole genome shotgun sequence.
ACCESSION   DATMED010000317 DATMED010000000
VERSION     DATMED010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644906"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /gene="pheT"
                     /locus_tag="VNP73_08175"
     CDS             <1..1620
                     /gene="pheT"
                     /locus_tag="VNP73_08175"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWL65935.1"
                     /translation="EFGQPLHAFDAALVTDRSIEVRRAKRGERMTTLDGIERELYPDD
                     LLIADTKRALAIAGVIGGADSEVGDGTEDVILESAYFDADSVAFTARRHLLRTEASAR
                     FERGADPEIVPVAAERAVRYIAETAGGNAPQEQVDEYPKPVEHRSIRLRPERTGVVLG
                     AGIQAEQQAAYLTALGMTVERSDGAFLVEAPTFRPDIKREEDLIEEVARIAGFERLPA
                     TLPPGRSGGLDREQRFERTLKRVLASLGIAEAWTTTFAGMHDLDGLLLPHDHPARKLV
                     RLANPMSDQEDALRSTLLPGLLRSVARNHAAHRESIALFEIGRVFEPSAEPLPLQPAI
                     LAAALAGERRPKRWSGAARDWDFFAAKGVLEAIARAAGVGNLRLAPTSGMPFHPTRGA
                     SVLLADSPIGSLGEIHPHVCAAFDVPEGTCSFEIALAPIIAALPDREQTEALPRHPAV
                     YLDLAIVVAEDVPAEKIAAAIDEAGKPELVGAVLFDVYRGEQVESGSKSLGYALELRV
                     TDRTLTDEEASAVRGRIVDRLAQSFDAQLRG"
     gene            1626..2558
                     /gene="argF"
                     /locus_tag="VNP73_08180"
     CDS             1626..2558
                     /gene="argF"
                     /locus_tag="VNP73_08180"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWL65936.1"
                     /translation="MNERDLLSIDDLNPEELGALLDRAADVKDDPAAFGDSLKGRNVV
                     LLFEKPSLRTRVSFEIGVTMLGGNAVVIGRDEIGLDSRESIEDVARVLSRWADAIVMR
                     TFGQDRLDRMADASSVPIVNALSDFSHPCQALSDLQTIREKKKQLAGTALAYLGDGNN
                     VAHSLMFGGAKLGMDVRIACPEGFEPFPQVVERCKELSASNGGHLSVGNVVAEAATGA
                     DVLYTDVWTSMGKEGEADSRKAEFERFQINPQVIAMADPDAIVMHCLPAHRGEEISAD
                     VIDGPRSVVWDQAENRLYAQMALLDWLIGNSQAN"
     gene            2536..>3045
                     /locus_tag="VNP73_08185"
     CDS             2536..>3045
                     /locus_tag="VNP73_08185"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWL65937.1"
                     /translation="MGTRRLTKDFYARDALTVARELIGCLFVRRIARGLISVRLVETE
                     AYRGRIDPGSHGYRGMTERTRVMYGPPGHLYVYFTYGMHWCANIVCARPGVCEAVLLR
                     AGEPVEGLGWMRRNRPGIKNDRLLASGPARLAQALGIDKKQNGASLLRGGQFWCAQDD
                     ATEGFRAKEI"
BASE COUNT          540 a          945 c         1030 g          530 t
ORIGIN      
        1 gagttcggac agcctcttca cgccttcgac gcggcgctcg tcaccgatag atcgatcgag
       61 gtgcgccgcg ccaagagagg cgagcgcatg acgaccctgg acggcatcga gcgcgagttg
      121 taccccgatg atctgctgat cgccgacacc aaacgcgctt tggcgatcgc cggcgtgata
      181 ggaggcgcgg attccgaggt gggcgacggc acggaagacg tcatcctcga gtccgcgtac
      241 ttcgatgccg actcggtcgc cttcaccgca cgccgccacc tgctgcgcac cgaggcatcg
      301 gctcgcttcg agcgtggggc cgacccggag atcgttcccg tggccgcaga acgcgccgtc
      361 cggtacatcg ccgagacggc cggaggaaac gcgccgcagg agcaggtcga cgaatacccg
      421 aagccggtcg agcacaggtc gatccgtctg cgaccggagc ggaccggggt cgtcctcggt
      481 gccgggatac aggccgaaca gcaagcggca tatctcaccg cgttgggcat gaccgtcgaa
      541 cgctccgacg gggccttcct cgtcgaggca ccgacctttc ggccggatat caagagagaa
      601 gaagatctga tcgaggaggt cgcgcgtatc gcgggcttcg agcgcctacc ggcgactctt
      661 cctcccgggc ggagtggtgg actggatcgg gagcagaggt tcgaacgcac cctgaagagg
      721 gtgcttgctt ctctggggat cgcggaggca tggacgacca ctttcgcggg catgcacgat
      781 ctcgacggtc tcctgctccc tcacgatcac cccgcgcgca aactcgtccg tctagccaat
      841 ccgatgagcg accaagagga tgcactgcgt tcgaccctgc ttcctgggct actccgttcg
      901 gttgcgcgga accacgcagc gcatcgtgag tcgatcgctc tgttcgagat aggccgcgtc
      961 ttcgagccga gcgcagagcc cctgcccctg caaccggcga tcctggctgc cgctctcgcc
     1021 ggggaacgca gacccaagcg ctggagtggg gccgcccggg attgggactt cttcgctgcg
     1081 aagggtgtgc tcgaggccat cgcacgcgct gccggcgtcg ggaaccttcg actcgctccg
     1141 acttcgggca tgccgttcca ccccactagg ggggcttcgg tgctcttggc cgacagcccg
     1201 atcgggagcc tgggcgagat ccatccccac gtctgtgctg cgttcgacgt acccgaaggc
     1261 acatgctcgt tcgagatcgc gctggcgccg atcatcgccg ctcttccgga tcgcgagcag
     1321 accgaggctc taccgcgcca cccggctgtt tatctggacc ttgcgatcgt cgtcgccgaa
     1381 gatgtcccgg ccgaaaagat cgcggccgcg atcgacgagg cgggcaagcc agaactcgta
     1441 ggggcggtgt tattcgatgt ctaccgcggt gagcaagtcg agtcgggcag caaaagtctc
     1501 ggttatgcgc tcgagctacg cgtgaccgac cgcaccttga ccgatgagga ggcgtccgcc
     1561 gtccgcggcc ggatcgtgga ccggctggcg cagagcttcg acgcccaact gaggggatga
     1621 cgaccatgaa cgagcgcgat ctcctgtcca tcgacgacct gaaccccgaa gagctggggg
     1681 ccctgctgga tcgcgccgcc gatgtgaagg acgaccccgc agctttcggt gacagcctga
     1741 aaggtcgcaa cgtcgtgctg ttgttcgaga agccctcttt gaggacgcgc gtctcgttcg
     1801 agatcggcgt gacgatgttg gggggcaacg ccgtggtgat cggccgggac gagatcggtc
     1861 tcgactcacg agaatcgata gaggacgtgg ctcgcgtgct ctcgcgctgg gccgacgcga
     1921 tcgttatgag aacgttcggc caggacagac tcgatcggat ggcggatgca tcttctgttc
     1981 cgatcgtgaa cgccctcagc gatttctcgc atccatgcca ggcgctctcg gatctgcaga
     2041 cgatccgcga gaagaagaag cagctggcgg gcacggcact tgcgtatctc ggggatggga
     2101 acaacgttgc ccattccctg atgttcggcg gtgcgaagtt gggcatggac gtccgcatcg
     2161 cttgccccga agggttcgag cccttccccc aagttgtcga gcgttgcaag gagctcagtg
     2221 cgagcaatgg ggggcacctg tcggtgggca acgtagtggc cgaagcggcg acgggagcgg
     2281 acgttctgta caccgacgtg tggacgagca tgggcaaaga aggggaagcg gactcgcgta
     2341 aggcagagtt cgagcgcttc cagatcaatc cccaggtgat cgcgatggct gaccccgatg
     2401 cgatcgtcat gcactgtcta ccggcccatc gaggcgagga gataagcgcc gatgtgatcg
     2461 atggaccgcg ctccgtcgtg tgggaccaag ccgagaatcg cctctacgcc cagatggccc
     2521 tactggactg gctgattggg aactcgcagg ctaactaagg acttctacgc gcgcgatgcc
     2581 ttgaccgtcg cccgcgagct gatcggttgc ctgttcgtca ggcgcattgc gcgtggactg
     2641 atcagcgtgc gcctcgtcga gaccgaggcg tacaggggac ggatcgatcc cggctctcac
     2701 gggtacagag ggatgaccga gcgaacgcgc gtcatgtacg ggccccccgg ccacctctac
     2761 gtctacttca cttacgggat gcactggtgc gccaacatcg tgtgcgcgcg tccgggcgtg
     2821 tgtgaagccg ttctgctgcg cgccggcgag cccgtcgagg gcctcgggtg gatgcgaagg
     2881 aatcgcccgg gcatcaagaa cgaccgcctg ctggcgtcgg gtccggctcg tctggcgcag
     2941 gcgctgggga tcgacaagaa gcagaacggg gcctcgttgc ttcggggcgg tcagttctgg
     3001 tgcgcccagg acgacgctac ggagggtttc cgagcgaagg agatc
//
LOCUS       DATMED010000318         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_269203, whole genome shotgun sequence.
ACCESSION   DATMED010000318 DATMED010000000
VERSION     DATMED010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269203"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1007)
                     /locus_tag="VNP73_08190"
     CDS             complement(75..1007)
                     /locus_tag="VNP73_08190"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWL65938.1"
                     /translation="MSDQVLAVTLAGLELKNPVLVASGTFGSGREGSTFVNLAKLGGI
                     IVKSMTVTPWKGKPTPRMCETPSGMLNAIGIQNKGVDYFIAEDFPWLLRQKATVIASI
                     AGNTVDEFVRVADKLRTGARGIAAVEVNISCPNLEDSSNMFSHSAESTSAVVGSVKKA
                     LPKISIFPKLSPNVTSLTEIAGAAIEAGADGLSLINTLFGMAIDVEHRRPKLAGVTGG
                     LSGPAIRPVAVRAVWDVHNAFPHVPIIGQGGVSTAEDALELILAGASAVAVGTANFVN
                     ARSTVGVAEGISEYMDRHEIVSVNDLVGAVKAEG"
     gene            complement(1010..1942)
                     /locus_tag="VNP73_08195"
     CDS             complement(1010..1942)
                     /locus_tag="VNP73_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWL65939.1"
                     /translation="MLLGTGEVLSHKKYGEHYHALTIVSPEIGEKVRPGQFINIKCGY
                     QQANILRRPFSVYRVHKRGGWASTIEIVFDIRGPGTTYLSHLRGHATVDLIGPLGRGF
                     TLPKRRAHCLLVGGGIGAAPLFFLADELRNEGHRVDVILGARDGGLLLNAIDARRLAS
                     VCVTTTEDGSVGTQGRVTDVLDDTIERCETEVVYACGPHPMLAAVSKVCAERKVPLQV
                     AVEELMACGYGVCMTCVMPMQRKRRSKEEPVEDTIVYARSCTEGPVFNGAAVVWNGES
                     ELVATDQAELSPAGVPSRSFPLFDTEDEHPPPGN"
     gene            complement(1942..>2922)
                     /locus_tag="VNP73_08200"
     CDS             complement(1942..>2922)
                     /locus_tag="VNP73_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWL65940.1"
                     /translation="DGTELYVGGVLEHIEEAGIHSGDSACALPPFTLGNSQIRQVIEF
                     TDAIARDLDVRGLINIQFAVKDEQVYVLEANPRASRTVPFTSKATGVPCAKVAARVMA
                     GATLAELRVEGLVPERAVGHEGIPHVAVKEAVLPFERFLGVDTVLGPEMRSTGEVMGI
                     DRNFGFAFAKAEASSGVRLPVKGTVFISVSNRDKRSVIFPAKRLHDLGFHLLATRGTA
                     EVLRRVGVEVEQIAKVSTPSAPGERNIVDKIAGGEIDMIFNTPFGRGARTDGYFIRTA
                     AVEAGVPCITTMAGMAAAVQGIEALIGGDVGVRSLQEYIKHDATQRKERG"
BASE COUNT          513 a          989 c          890 g          530 t
ORIGIN      
        1 ccgaatctcg cgggcgtccg cgaagtcgag cgccacgcat accgggttac gcgcagaatc
       61 agagcgcgcc ttggtcatcc ctccgccttc accgccccga ccaggtcgtt caccgatacg
      121 atctcgtgcc ggtccatgta ctcggagata ccttcggcca cacccacggt cgagcgcgcg
      181 ttgacgaagt tagccgtccc caccgcgacc gcgctcgctc cggccaggat gagctcgagc
      241 gcgtcctccg cggtggagac gccgccctgc ccgattatcg gaacgtgagg aaacgcgttg
      301 tgcacgtccc acaccgctcg caccgcgacc ggccggattg ccggccccga gaggccaccg
      361 gtaacgccgg cgagcttggg acggcggtgt tcaacgtcaa tggccatgcc gaagagtgtg
      421 ttgatcagcg acaagccgtc ggctcccgct tcgatggccg ctccggcaat ctcggtcagg
      481 gaggtcacgt tcggagacag cttcggaaag atcgagatct tgggcaacgc cttcttgaca
      541 ctcccgacga cggccgaggt tgactcggcg ctgtggctga acatgttgct ggagtcctcg
      601 aggttggggc aggagatgtt gacctcgacc gccgcgatgc ctctcgctcc cgtgcgcaac
      661 ttgtccgcga cccggacgaa ctcgtcgacc gtattgccgg ctatggaggc gatcacggtc
      721 gccttctggc gcaagagcca cgggaagtct tccgcgatga agtagtccac ccctttgttc
      781 tggataccga tcgcgttgag catccccgaa ggcgtctcgc acatccgcgg cgtcggcttg
      841 cccttccacg gggtgacggt catcgactta acgatgatgc ctccgagctt ggcaaggttg
      901 acgaaggtcg aaccttcgcg gccggatccg aaggtgccgg acgcgacgag gacgggattc
      961 ttgagttcga gaccggcgag ggtcacggct agtacctgat cgctcatcgt caattacccg
     1021 gcggagggtg ctcgtcttcg gtgtcgaaca acgggaaact gcggctgggg acgcccgccg
     1081 gcgagagttc ggcctgatcg gtcgcgacca gttccgactc gccgttccag acgacggcag
     1141 ctccgttgaa gaccgggccc tccgtacagg agcgcgcgta gacgatcgtg tcctctaccg
     1201 gctcctcttt gctacggcgc ttgcgctgca tcggcatcac acaggtcatg cagaccccgt
     1261 acccgcaggc catcagttcc tcgaccgcaa cctgcagcgg gacctttcgc tccgcacata
     1321 ccttcgagac cgcggccagc atcgggtgcg gtccgcacgc ataaacgact tcggtctcgc
     1381 atcgctcgat cgtgtcgtcg agaacgtcgg taacccgtcc ctgcgtgccc acgctgccgt
     1441 cttccgtagt cgtaacgcaa accgacgcga gccgacgagc gtcgatcgcg ttcagcagca
     1501 gtcccccatc ccgcgcgccc agaatcacgt ccacgcgatg tccctcgttg cggagttcgt
     1561 cggcgaggaa gaacagtgga gccgccccga tcccaccgcc cacgagaagg cagtgggcgc
     1621 gccgcttggg gagtgtgaac ccgcgtccca ggggaccgat cagatccacc gttgcgtggc
     1681 cccgcagatg ggacaggtag gtggtcccgg gaccacggat gtcgaacacg atctcgatcg
     1741 tcgaggccca cccgccgcgt ttgtgcaccc gatagaccga gaagggtcgc ctcaggatgt
     1801 tggcttgctg gtaaccgcac ttgatgttga tgaactggcc cggcctaacc ttctcgccga
     1861 tctcggggct gacgatcgtc aacgcgtgat agtgctctcc gtacttcttg tgggaaagaa
     1921 cctcacccgt cccgagcagc atcagcctcg ctccttgcgc tgggttgcat cgtgcttgat
     1981 gtactcctgg agcgacctga cacccacgtc gccccctatc aaggcctcga tgccctgtac
     2041 cgctgcggcc atccccgcca tcgtggtgat gcagggcacg ccggcttcga ccgcggccgt
     2101 gcggatgaag tagccatcgg ttcgagcccc gcgtccgaac ggtgtgttga agatcatgtc
     2161 gatctcaccg ccggcgatct tgtcgacgat gttcctctct ccgggggcag aaggtgtcga
     2221 cacctttgct atctgttcga cctcgacacc cacgcgtctc aggacctcgg ccgtacccct
     2281 cgtcgccaga agatggaacc cgaggtcgtg caaacgcttg gccggaaaga tcaccgagcg
     2341 cttgtcccgg ttcgaaaccg agatgaacac cgttcccttc accggtagcc gcacgcccga
     2401 cgacgcctca gccttcgcga aggcgaaccc gaagttgcga tctatcccca tgacctcgcc
     2461 cgtggagcgc atctcgggtc cgaggacggt gtcgacgccg aggaatcgct cgaacggcag
     2521 gacggcctcc ttgacggcca cgtgcggtat cccctcgtgg ccgaccgctc tctcgggaac
     2581 gagcccctcc acgcgcaact cggccagcgt ggcaccggcc atcacccgcg cggcgacctt
     2641 cgcgcacggc acgccggtcg ccttggatgt gaacggaacc gtccgggatg cacgcgggtt
     2701 ggcctcaagg acgtaaacct gctcgtcctt gaccgcgaac tggatattga tgagaccacg
     2761 cacgtccaga tcgcgcgcta tcgcgtcggt gaactcgatc acctgacgga tctgggagtt
     2821 gccgagagtg aacggaggta gcgcgcatgc cgagtctccg gaatggatac cggcctcctc
     2881 gatgtgctcg agcacccctc cgacgtaaag ctctgtgccg tc
//
LOCUS       DATMED010000319         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1016116, whole genome shotgun sequence.
ACCESSION   DATMED010000319 DATMED010000000
VERSION     DATMED010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016116"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VNP73_08205"
     CDS             complement(<1..203)
                     /locus_tag="VNP73_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWL65941.1"
                     /translation="MSHSTKRDPRDIILQPVVSEKSYSLLDSNAYTFLVHPKANKTEI
                     RQAVEQIWDVRVTKVNTNNRRGKT"
     gene            complement(200..844)
                     /gene="rplD"
                     /locus_tag="VNP73_08210"
     CDS             complement(200..844)
                     /gene="rplD"
                     /locus_tag="VNP73_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWL65942.1"
                     /translation="MSLKAKVLKASGSTSGSYDLPDDLFGGDINVPVMHQVVTAQLAE
                     ARSGTASTKTRSEVRGGGTKPWRQKGTGRARHGSIRSPIWVGGGVTFGPNGRKYTKRV
                     NKKMRKSALRSALADKAENGRVWVLDGFDEPRTKAAAEFLQTADITGRTLVVLDPDDE
                     GAVTVDRAFRNLDSVAFALYGAIATYDVLVADDVIFTRSALDRLSSSKTEEASS"
     gene            complement(841..1497)
                     /gene="rplC"
                     /locus_tag="VNP73_08215"
     CDS             complement(841..1497)
                     /gene="rplC"
                     /locus_tag="VNP73_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWL65943.1"
                     /translation="MVAEVKGILGRKVGMTQVFDDGGHAVPVTVVEAGPCSVAQVKTA
                     DKDGYSAIQLAFGEPKRVNQPTAGHFAKAGVDPSRHVVELRLDDVGDYSLGSEIKADV
                     FEEGEMVDVVGVTKGKGFTGAMKRHHFSGLGASHGTERKHRSPGSIGGASTPARVFRG
                     QKMPGHLGHERVTTLNLKVIKVDPERNLMLIRGAVPGPRGGLVMVRSAIRLRKQREAG
                     "
     gene            complement(1604..1903)
                     /gene="rpsJ"
                     /locus_tag="VNP73_08220"
     CDS             complement(1604..1903)
                     /gene="rpsJ"
                     /locus_tag="VNP73_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWL65944.1"
                     /translation="MKIRIRLKAYDHEVIDQSAKKIVETVTRTGASVNGPIPLPTERS
                     VYTVIRSPHKDKDSREHFEMRTHKRLLDIVNPTPKTVDSLQRLDLPAGVDIELKL"
     gene            complement(1933..>2250)
                     /gene="tuf"
                     /locus_tag="VNP73_08225"
     CDS             complement(1933..>2250)
                     /gene="tuf"
                     /locus_tag="VNP73_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016960638.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWL65945.1"
                     /translation="GQVLAKPGSVTPHKKFQAQVYVLSKDEGGRHTPFFNNYRPQFFF
                     RTTDVTGTIELPSGTEMVMPGDNVAMTVELGKPIAMDEGLRFAIREGGRTVGAGRVTK
                     IIE"
BASE COUNT          370 a          727 c          661 g          492 t
ORIGIN      
        1 gtcttaccgc ggcgattgtt cgtgttgacc ttggtcaccc gcacgtccca gatctgctcg
       61 accgcctgcc ggatctcggt cttattggct ttgggatgga cgagaaacgt gtacgcgttc
      121 gagtcgagca gcgaatacga cttctcgctg acgacgggct gaaggatgat gtcccgcgga
      181 tcgcgtttgg tcgagtggct cacgacgatg cctcctcggt cttcgatgag ctaagacggt
      241 cgagtgccga acgagtgaag atcacatcgt cggcaacgag cacgtcatag gtcgcgatcg
      301 ctccgtagag ggcgaacgca accgagtcga gattcctgaa cgctcggtcc actgtcaccg
      361 ctccctcgtc atcgggatcg agcacgacga gcgttcttcc ggttatgtcc gccgtctgaa
      421 ggaactcggc ggcggccttc gtacgcggct cgtcgaaccc gtcgagcacc cacacgcgac
      481 cgttctcggc cttgtcggcg agagccgaac gaagagcgga cttacgcatc ttcttgttga
      541 cccgcttggt gtacttgcga ccgttgggtc cgaacgtgac gcctccaccg acccagatcg
      601 ggctgcggat cgatccgtgc ctggcccggc cggtaccttt ctgacgccac ggcttggtac
      661 ccccgcctcg gacctcggaa cgagtcttag tggatgcggt cccggagcgc gcctcggcta
      721 gctgggccgt cacgacctgg tgcatcacgg ggacgttgat gtcgccgccg aaaaggtcgt
      781 cgggcaagtc atagctgccc gaggtcgagc cggaagcctt cagaaccttt gctttgagac
      841 tcatccggcc tccctttgct tccttagcct gatcgcgctg cggaccatga ccaggccccc
      901 gcgagggccg ggcaccgcac ctcgaatgag catgaggttg cgctcggggt cgaccttgat
      961 caccttcaga ttgagagtgg tcaccctttc gtggccgagg tgaccgggca tcttctgtcc
     1021 ccggaacacg cgagccggcg tcgaggcgcc gcctatcgat ccgggagacc ggtgcttgcg
     1081 ctcggttccg tggcttgcac caagtccgct gaagtggtga cgcttcatcg ccccggtaaa
     1141 gccttttccc ttggtaacgc cgacgacgtc caccatctcg ccctcttcga agacatcggc
     1201 cttgatctcg ctcccgagcg agtagtcgcc gacatcatcc aggcgaagtt caacgacgtg
     1261 gcgcgacggg tcgacgccgg ccttggcgaa gtggccggcc gtcggctggt tgacgcgctt
     1321 gggctcgccg aaggcgagct ggatcgcgct gtatccgtcc ttgtcggcgg tcttgacctg
     1381 ggcaacgctg cacggcccgg cctcgacgac agtcacgggc accgcgtgcc ctccgtcgtc
     1441 gaaaacttgg gtcatgccga ccttgcggcc aagaatgcct tttacctctg ccaccaagaa
     1501 acctctaagt ctctccgcag atcgtcgaga cgatctgctt cttgcggaac caatctcgat
     1561 tccacttgtc tccgtactcc tgtcagttct cctatttctt ctgttacagc ttcagttcga
     1621 tgtcgactcc tgcaggaagg tccaacctct gcagtgagtc caccgttttt ggtgttggat
     1681 tcacgatgtc gagcaacctc ttgtgcgtgc gcatctcgaa gtgctcgcgc gagtccttgt
     1741 ccttgtgggg cgaacggatc accgtgtaaa ccgaacgctc cgtcggcaac gggatggggc
     1801 cgttgacgct tgcgcctgtg cgcgtgaccg tctcgacgat cttctttgcc gactggtcga
     1861 tgacctcgtg gtcgtacgcc ttcagcctga tacgtatttt cattcccgat gccacgtgag
     1921 ctgttctctt ctctattcga tgatcttcgt gactcggccg gcgccgacgg tacggccacc
     1981 ctcacggatg gcgaagcgca gaccctcgtc catcgcgatc ggcttgccca actccaccgt
     2041 catcgccacg ttgtcacccg gcatcaccat ctccgtcccc gacgggagct cgatcgtgcc
     2101 cgtcacatcc gtcgtgcgga agaagaactg cggccggtag ttgttgaaga acggtgtgtg
     2161 tcgcccaccc tcatccttcg agaggacgta cacctgcgcc tggaacttct tgtgcggagt
     2221 gacgctgccc ggcttggcaa gcacctggcc
//
LOCUS       DATMED010000320         4239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_272599, whole genome shotgun sequence.
ACCESSION   DATMED010000320 DATMED010000000
VERSION     DATMED010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272599"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VNP73_08230"
     CDS             complement(<1..328)
                     /locus_tag="VNP73_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65946.1"
                     /translation="MAVDYLDKAIEYLEKVNAGLEPELMPARDTQTVLKKCARARARA
                     LLDFTIASLSRKQDDPERLARVTGTSIGKAKETVATGKAIADSGELSMALQQGQVSLD
                     QASEIAK"
     gene            451..804
                     /locus_tag="VNP73_08235"
     CDS             451..804
                     /locus_tag="VNP73_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWL65947.1"
                     /translation="MARAATTTDAFNAVAEPRRRQILDVLAGGERPVNDLVTVLGLAQ
                     PQVSKHLKVLREVGLVDVRDEGRHRVYSLNGQPLRPIHEWVKNYERMWSERFEALDEV
                     LEELVREEEADGGNN"
     gene            788..1285
                     /locus_tag="VNP73_08240"
     CDS             788..1285
                     /locus_tag="VNP73_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWL65948.1"
                     /translation="MVATTSSQSAKVTLPADDQLLITREFAAPKHLVYRAITEPELIK
                     RWWNAKRGEVTLAEHDVKVGGKWRQVMVTPDGFEVGFHGEYREIVPNERIVSTEVYEG
                     APGGDEGGSVNTVTLTEIDGRTTMTVLCKFPSKEVRDMVVESGMEAGMQDAYDLLEEV
                     AVSLI"
     gene            complement(1335..1640)
                     /locus_tag="VNP73_08245"
     CDS             complement(1335..1640)
                     /locus_tag="VNP73_08245"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWL65949.1"
                     /translation="MSVWSLILVLAAWTNALRLGGAFLLKRATVPPTAEKVLNNITPA
                     VLGALVVAGMFTSGRDLVLDERFFGFVAAGAALIGKLPPLAVIVVAAGATALARALT"
     gene            complement(1637..2296)
                     /locus_tag="VNP73_08250"
     CDS             complement(1637..2296)
                     /locus_tag="VNP73_08250"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWL65950.1"
                     /translation="MALPPGYLRADGVITAFALAVIVFGATFGVLARAAGLTAPEAIF
                     MSVLVFAGASQFAVIGAISAGAGTWVALVAGALLNLRLFALALAVAPGLSKNGWARAV
                     QSYLVTDETAAVAARPDGSVDEKRFVTAGVWVAGAWIVGTTIGALGGSAVSDPLVWGL
                     DAAFPGGFLALLAPRLLKDRVARKVALVGAVTCLALTPFVPLGIAPLVAALAAFLAVK
                     K"
     gene            complement(2296..2880)
                     /locus_tag="VNP73_08255"
     CDS             complement(2296..2880)
                     /locus_tag="VNP73_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HWL65951.1"
                     /translation="MAGTLGAVGTVIGLVRALPQLIRLLRTKDAHGVSLDSAATSSIV
                     SFAWMAYGLLSDQFAVVLATGLSATVFAVITLVALRFGRHVRELRAAPYCLAIVLIVP
                     LAAGGGGLGTILAVSALVANVPQVVVAYRERDLTGLSPSTWALSASDGAVWLTYGIVT
                     GDIPIMVNNFFQFSTSSAIVVRRLTWGRPQPSEA"
     gene            complement(2912..3547)
                     /locus_tag="VNP73_08260"
     CDS             complement(2912..3547)
                     /locus_tag="VNP73_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWL65952.1"
                     /translation="MSFLSSPELLVLHAARIKGRVDADAIARRYSLVREEVEELLLDH
                     EARGFVHRVEFADIHGWFITESGRDEIHGLLQVEVEEAGARPVVAETHTAFLPLNKRF
                     MDEITRWQVRPMPWDRMAMNDHTDGRWDEKVLVALTSLARRLRPLEEQLSARLARFGG
                     YADRIDAALARVDRGEKRWVDEPGIDSCHMVWFELHEDLLATLGIDREAEV"
     gene            3590..>4239
                     /locus_tag="VNP73_08265"
     CDS             3590..>4239
                     /locus_tag="VNP73_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWL65953.1"
                     /translation="MNESRWGAVLEAVTGPALKYFASLPDRHVYRPADPNEIRAIIGG
                     PMPGYATPPEEVVAALARDLEPFVTAHSCGRFFGFVIGGLHPAAYGAELLAATWDQNA
                     GLYAVTPGVAMVEEVALEWLLDLLDLPRDASAGFVTGGQMANFTCLAAARDAVLRDVG
                     WDVEADGLQGAPKLNVVVKSEKHATVSRALRYLGLGHTSAIEVASDEQARIDVPDLA"
BASE COUNT          842 a         1381 c         1303 g          713 t
ORIGIN      
        1 ctttggcgat ctcggatgcc tgatccaacg agacctgtcc ctgttgcaac gccatactca
       61 gctcacccga gtcagcgatg gccttgcccg tggccacggt ctctttcgcc ttcccgatcg
      121 acgtcccggt cactcgggca agccgttcgg gatcgtcctg cttccgagac agagacgcga
      181 tcgtgaagtc cagcagcgct cgggctcggg cgcgggcgca tttcttgagc accgtttggg
      241 tgtcccgcgc cggcatcaac tcgggttcga gacccgcatt gaccttctcg agatactcga
      301 tcgccttgtc caggtaatcg actgccatgc ccccactttg acgagagggt gtgacacttt
      361 tcgccccgat ttgggccgat tcccagaaaa cctctcccct tccctgaggc cccggcttga
      421 catattccta tatcggcata tgatgccctc atggcacgag cagcaacgac tacggatgcg
      481 ttcaacgcag tggcggagcc ccgacggcga cagatcctgg acgtcctcgc cggtggggag
      541 cgcccggtga acgacctcgt cacggtgctc ggcctggctc agccgcaggt ctccaagcac
      601 ctgaaggtgc tgagggaggt gggattggtg gacgttcgtg acgagggccg ccaccgcgtc
      661 tactcgctca acgggcagcc gctcaggccc atccacgagt gggtgaagaa ctacgagcgc
      721 atgtggagcg aacggttcga ggcgctcgac gaggtactgg aagagttggt tcgagaggag
      781 gaagcagatg gtggcaacaa ctagcagtca gtcggcgaag gtgacgctcc ctgcggacga
      841 tcaacttctg atcacacggg agttcgcggc cccgaagcac ctggtctacc gggcgatcac
      901 cgagccggag ttgatcaaga ggtggtggaa cgcgaagcgc ggcgaggtca cccttgccga
      961 gcacgacgtg aaggtcggcg gcaagtggcg ccaggtgatg gtcacgcccg acgggttcga
     1021 agttggtttc cacggtgagt accgcgagat cgtccccaac gagcgcatcg tctcgaccga
     1081 ggtgtacgaa ggggcgccgg gcggcgacga aggcggttcg gtcaacacgg tcacgctaac
     1141 ggagatcgac ggtcgcacga cgatgacggt tctctgcaag ttccccagca aagaggtccg
     1201 cgacatggtc gtcgagtccg gcatggaagc cggcatgcag gatgcatacg accttctgga
     1261 ggaagttgcg gtttcgctga tctgatacgc atcagcgtca cctgggggtc atccgatcgg
     1321 atgacccccg tttctcatgt aagcgcccgt gccagcgcgg tcgcgccggc ggccacgacg
     1381 atcacggcga gcggaggcag cttccctatc agcgccgcac cggccgcaac gaatccgaag
     1441 aaccgctcgt cgaggacaag gtcgcgtcct gaggtgaaca tccctgcaac gaccagggcc
     1501 ccaagaacgg ccggtgtgat gttgttcagg actttctcgg cggtgggagg cacggttgca
     1561 cgcttgagga ggaaagcgcc gccgagacga agcgcgttcg tccatgccgc cagaacgagg
     1621 atcagcgacc agacgctcac ttcttcaccg ccaggaatgc agcgagggct gcgaccagag
     1681 gagctatccc gagcgggacg aagggtgtca gcgccaggca tgtcacggct cccaccagcg
     1741 cgaccttgcg agcaacccga tccttcagca gacgaggtgc aagcagcgcg aggaaccctc
     1801 ctgggaaggc agcatccaga ccccacacga gcgggtcgct cacggccgag cctcccaacg
     1861 cgccgatcgt cgtgccgacg atccaggcgc cggccaccca gacgccggcg gtgacgaatc
     1921 gtttctcgtc gacggatccg tcgggacgtg ctgcaaccgc cgccgtctca tccgttacca
     1981 agtagctctg aaccgcgcgt gcccatccgt tcttcgacaa tccgggagca accgcaaggg
     2041 ccagcgcgaa caaccgaagg ttgagaagag cacccgcaac gagagcgacc cacgtacccg
     2101 cacctgccga gatcgcccca atgacggcga actgcgaagc accggcgaat acaagaacgg
     2161 acatgaagat cgcttccgga gcggttagtc cggccgctcg agccagaacg ccgaacgttg
     2221 caccgaacac gatcactgcc agtgcgaagg ccgtgatcac gccgtcggcg cgcaggtatc
     2281 cgggcggcag tgccactacg cttcagaagg ttgaggccga ccccaggtca agcgtcgaac
     2341 cacgatcgcc gaactagttg agaattggaa gaagttgttg accatgatcg ggatgtcccc
     2401 ggtgacgatc ccgtaggtca gccacaccgc tccgtccgat gccgacagcg cccaagtcga
     2461 gggagacaat cccgtcaggt ctctctctcg gtaagcgacg acaacctgag gcacgttggc
     2521 caccagcgca ctcaccgcaa ggatcgtgcc cagtcccccg ccccccgccg cgagcgggac
     2581 gatcaacacg atcgcgaggc agtacggcgc ggccctgagt tcccgaacgt ggcgcccgaa
     2641 tctcagagcg accaaggtga tcaccgcgaa caccgtggca gacagtccgg tcgccagaac
     2701 cacggcgaac tgatccgaaa gaagaccgta ggccatccac gcgaagctga cgatcgagct
     2761 ggttgccgcc gagtccaacg agaccccgtg ggcatctttc gttcgaagaa ggcggatcag
     2821 ctgaggaagg gcgcggacga gcccgatgac cgtgccgaca gcgccgaggg ttcccgccat
     2881 tcctagggtc tatgcctttc ggttcgcacg ctcaaacttc cgcctcacgg tcgattccca
     2941 gcgtggccag cagatcctcg tgcagctcga accagaccat gtgacaggag tcgatccccg
     3001 gctcgtccac ccacctcttc tcgccccgat cgacccgcgc cagtgccgca tctatgcgat
     3061 cggcgtaacc gccgaagcga gccagtctgg ccgaaagttg ctcttcgagc ggacgcaatc
     3121 tgcgggcgag gcttgtcaac gcgacgagca ctttctcgtc ccaacgtccg tcggtgtgat
     3181 cgttcattgc cattcgatcc cagggcatgg ggcggacctg ccaccgagtg atctcgtcca
     3241 tgaatcgctt gttcaaggga agaaaagccg tgtgtgtttc cgccaccacc ggacgggcgc
     3301 cggcttcctc gacctcgact tggagcagcc cgtggatctc atcgcgtccc gattcggtga
     3361 tgaaccaccc gtggatatcc gcgaactcaa cccggtgtac gaacccgcgg gcctcgtggt
     3421 cgagcagcag ttcctccacc tcctcacgaa ccagcgagta acgccgcgcg atcgcatccg
     3481 cgtcgaccct gcccttgatg cgagcggcgt gcagcacgag caattccggc gacgacaaga
     3541 aactcaccgc ggcaacctat aagttcgacc taaaagtggg aggttgatcg tgaacgaatc
     3601 aagatgggga gccgtcctcg aggccgtaac cggacctgcc ctcaagtact tcgcctcgct
     3661 ccctgatcgg cacgtctacc gtcctgcaga tccgaatgag atccgcgcca tcatcggcgg
     3721 ccccatgccc ggatacgcga cccctccgga ggaggtcgtc gccgccttgg ctcgagacct
     3781 cgagcccttc gtaaccgccc actcatgtgg ccgtttcttc ggattcgtca tcgggggact
     3841 tcatcccgcc gcctacggcg ccgagctact ggcggcaact tgggatcaga atgccggcct
     3901 ctatgcagtc acacccggcg tcgccatggt cgaagaggtc gccttggaat ggctcctgga
     3961 tctcctagat ctcccacggg atgcttctgc cgggttcgtc accggaggcc agatggccaa
     4021 cttcacttgc ctggcggccg cccgtgacgc cgttctccga gatgtcgggt gggacgtcga
     4081 ggccgacggc cttcagggcg ccccaaaact gaatgtggtc gtgaaatcag agaagcatgc
     4141 aaccgtttcc cgcgcgctgc gttacttggg gctgggtcac acgagcgcga tcgaggtggc
     4201 ctcggatgag caggcccgga tagacgttcc agacctggc
//
LOCUS       DATMED010000321        14945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3719917, whole genome shotgun sequence.
ACCESSION   DATMED010000321 DATMED010000000
VERSION     DATMED010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719917"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VNP73_08270"
     CDS             <1..659
                     /locus_tag="VNP73_08270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL65954.1"
                     /translation="KSAAADFLAQRARGGPALELGIGTGRVALLLKERGVAVHGIDAS
                     EKMVERLREKPGGGDIPVTVGDLADVGVDGSYPLIYVPFNTLFALESQEDQIRCFKNV
                     RDRLTDDGVFVTETFVPDVTRYERNQGTSVEDIRSDRVFVSFSQHDPVTQTSMTQHVF
                     FSSKGIELFPVFLRYAYPAEMDLMARVAGLALRERYGDWRMAPFNSSSTAHISVYGPE
                     "
     gene            complement(661..1362)
                     /locus_tag="VNP73_08275"
     CDS             complement(661..1362)
                     /locus_tag="VNP73_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HWL65955.1"
                     /translation="MRRGGAPSAIAVLFVLIAWAGLYVFLLATDRTSLYLSARTAWLV
                     PVGAIILIVSVVGYLVTRPDSPALSNRSVSGLALVALPVVVVIALPPASLSSFAASRR
                     SSFASAAFVTSVEDISTGSLSLADVAGAVRSDEAMAALTARAGAEVEFVGFVSRDPGM
                     PADEFLLTRFLISCCVADALSVQVRIVDAPAGEFKQDQWVQVTGNLFPLNNEVVVQAE
                     DVVKVPRPKHPYLNA"
     gene            complement(1359..2357)
                     /locus_tag="VNP73_08280"
     CDS             complement(1359..2357)
                     /locus_tag="VNP73_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWL65956.1"
                     /translation="MAQAALTPIDTRSAWRRSFVAVAALAGIAVVLNLLNPARIPALQ
                     NFLLVFSSLLIEAMPFILLGAVVSAVIEVFVPRSVFDRLASLPRAVQLPAAGLTGFAF
                     PVCECGSVPVARRLAHKGLSAGAAVTFMLAAPILNPVVLVSTIIAYRGRDILVPIVVG
                     RATLGLLAALAVGWIVANKTSGELLSATAEDDEHAHHADSRAGSFFSHLTGDFIFMGR
                     FLVLGAAVAATMQTFVPQTWIASIADTPFLSLIAMMALAFVLSLCSESDAFVAASFVQ
                     FGVGAQLAFLVFGPLVDTKLAFLYSGTFSREFLRTVVLVVGAVVFAGTLWVEVIVG"
     gene            complement(2357..3724)
                     /locus_tag="VNP73_08285"
     CDS             complement(2357..3724)
                     /locus_tag="VNP73_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65957.1"
                     /translation="MHPDRRVVSILALLALIAIPAAVLRVLCLGRACEEERTRAAQVP
                     FCSLPASVRDRIAAGYRDGRSPDVLAVTEPTVVLTAGSSSQVARDNTVWPALGDGPDL
                     VPIVFYGHGVATQGSITAGIEDISPTAAEIIGFDIPHPEVRAGRPVEGVASGDPPRLF
                     VYVILEGASPEDLATARFVRERSRDGAGTFDGAVASLPLDPAAIMATIGTGGLPVEHG
                     ITGSLVRDDDGRVGHAWNAPPVGPVGPGTVIATLADDLDEDTRQRSLIGLLGRKPQDR
                     GLIGGEWYIDVDEDYVEYSGGGHNATLVAESFPLALRTGFGRDEVPDLFAVVLSGTVE
                     PGTKQYLGKALADIERAANDASGGSAAFVLTSLPVGGTSDPDTPVSPGFVPLVKDQIG
                     TRLDADVVEASVAGGFFLDQDALAQEEISRQEVIAALRSTEIGGEPVFADAFPGFAVA
                     LARFC"
     gene            3752..5506
                     /locus_tag="VNP73_08290"
     CDS             3752..5506
                     /locus_tag="VNP73_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65958.1"
                     /translation="MRDAGNRLWIVIGVAVVAAALAGYQLLPERGVPAGAPTLDEMAS
                     GLGGPIMEHLNRGHVPGRSGELLLVPKPHHYIIGKWDLTTLGTDAPTLTNSHPNPWAY
                     IARVPIVLYGPPFVAEDRRVERSVDIADMAPTYAALLGMQALDADGEVLTEAVEDSPD
                     RAPKVIFTIVLDGGGWNVLQEHPNSWPTIQSLREGGTDYINATIGSAPSITGALHATF
                     GTGVYPVTHGIPGNQMRGPDGLNTDAWKQNADPEYLLVPTVSELWDEQNANEPIVGTV
                     SYEGWHLGMIGHGAQREGGDKDIAVLWEAETNEWWINDDFYELPDYLAETDLDTLERY
                     EEELDERDGLPDGLWFGHDLEELRDPVVRPGSSAFARFTGDAVVDVMRREKLGVDDVP
                     DMFWVEMKMPDFAGHRWNMIGPEEADVLKVVDDQIARFKRELDRLAGPGNYVIAISAD
                     HGQQPLPELEGGWRINSNELLADIEARFGNVVEKITTVDIFFEPEAMENGDVDLEDVA
                     RFLGTYTVGDNIPRGAVGAGFVPVERLDDTIFAGAFPTNYLASPPMEFTEPSCEYPTE
                     SNLFGPPGPGSTCASVIE"
     gene            5506..6774
                     /locus_tag="VNP73_08295"
     CDS             5506..6774
                     /locus_tag="VNP73_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL65959.1"
                     /translation="MLKRGLQLLVQKRDFGLLMLVQFAAQAGDAIIQTSLAKFIVFGG
                     NKGLDVEAAPSPDVLLSMVLFLFVPYTIISPFLGVVIDRWDRRRLLFGINALRAAGAI
                     LVGFIGIERVGNLTLLIVFLLTLAATRIVLATKAAALPTALGDKDLVEGNAVSQLGGA
                     LFQLGGGGVALVATLLLPAEPVAVTGGIVCAVAAAIALRIRHAGETRESSTLGREIAR
                     VLSSIWAGLREVARTPKAGAAITTYFWLRFLWSLSIVGIGFIAKELVAGNDNVRLVLT
                     AGGGAAGAVLGFLLADKLRARVPNTARIVLLSSALAGLGTAALGALEIKAAIVMLTFF
                     LGLGFFLGKISLDTLVQEALGDDFRGRAFSLYDIAYNVAWVMAAVIMKIWWTDLHGVL
                     IATMGVVFLIGLAALAMWYRAAGLLALQRS"
     gene            6778..8418
                     /locus_tag="VNP73_08300"
     CDS             6778..8418
                     /locus_tag="VNP73_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL65960.1"
                     /translation="MSRRLFSGSRLPILGAGLALVTVVLAMTGVLSPAPEARSFESQF
                     CGLPGPWRERIRRGYFGPRSGQIAILPRYPAYSGTSEGGWSHSGPWGYLQRVPLVFYG
                     PGVIPAEGDVERPVTVADIAPSLMRLMRGTLNTRDSDALSEALPRGPALVGNPSPRLI
                     VVSVWDGGGWNVLERWPEAWPNLRRLMDEGTSFTNATVGSSPSVTPSVHTTIGTGVFP
                     WHHGITGIPVRDENGEVVDSFLKGDSSRFIQAPAVAELWDEHHDNRAEIAMVGYEPWH
                     LGMIGLGAEDRTGDKDVAVWLDTETNEWITNPAHYSKPDVFLDRTVLDEESRALDAVD
                     GEVDRSWRSLDLDDPARIEETPAFIAYHGHVMSEFIEQEGYGRDRITDLLFTNFKQID
                     RLGHYFNMASEEVRQALEESDRQLGELVRELDTLVGRGRWAVVVTADHGQQPDARAVD
                     GYGIDPKEVARDVDAEFGPITRAVWPTEVFLHDDVMSREGVTLEEVSRFLSDYRMAEN
                     TQRPDMLVGGAGRFGPRDTLFELAMPSRLIPGLACGRR"
     gene            8432..10474
                     /locus_tag="VNP73_08305"
     CDS             8432..10474
                     /locus_tag="VNP73_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65961.1"
                     /translation="MRADSAETSSAATGAPKDPISRALGHPVAYVATAVILLAVFAWP
                     FLSDAARVAPTKDPAFYTWRTEAFLTEDPATLLGIEGPQGMFAAGYRVTAPILGGLLR
                     HVAGIGSLHVTVFLMVSLPVLIALALAGFAHSRFRDPLVWHSTAIGSAGLLLTPPFVG
                     YLDNVLCLFFLAASLFFIEGAQRSWVDRALFFVLLLLAGLTHPTTLVIFVATLGLMAG
                     LKLLLSKFDIKATLVSDGWMLATGAASVVATVAIWTFGLWGPSVSLTEAALPPPYGAS
                     FFIDRMMLWIGTMRPVLNGPLFLVGLVGLLAGGRELLASTHVRAILSWLAPLAGLFGF
                     IAGLTYPYYRFFNTTLSWVLLVGIGGGFAARFFLSGGRTGAARVAMSLGAVAVGVILV
                     TNITHGLDVSGWTKVKNQWLEPEARADLDELRQALALEDPDRPVVFVIDDELPEPFQI
                     YGASKLYGNTSRYGLPPGQIDRGYLYLGSIENLIAGEPTSTGDETYDPLSTALLEDTT
                     QGIQRSGLDPVIVIAKIFNPAGANAQVAAGEEDLPESVTSQLTSQVGVGRSVWTLHDG
                     SIYEWTDAGRVGLERSGLQPEDPNAIVNLLQVLFGLVLIALPGLAWYLWSNDDRATPR
                     ALALVPAVGMGVVTLGGVVVVAIARAPFSAPVAFATIAVGLVLAVAMRFLPRHEVA"
     gene            10471..12168
                     /locus_tag="VNP73_08310"
     CDS             10471..12168
                     /locus_tag="VNP73_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWL65962.1"
                     /translation="MIAALLERLPTERPGRNGRILWVLTLIAGAAGIAGWATQTLVGG
                     VVALWSLAAAAALVAMVLPFRYALVSPLYMGVAGWLVDMLPFVILIGWGVVSLRWGIK
                     VLRERRWPRGGRLIWIPIGLVFWTALGVVVITRLDFRHFLLLLGIQVIASAAVLATLD
                     SVRGVSERMDLVRALLGFVVLLTVAVFAQWIGIDIETLQNGATRKRVEAAYGVDAFPN
                     NIGMIKYARSINAGALEKQREMERVQARTAGLPDFEVFRPKFQAYENSLVVRFEGSAR
                     PYEQALGEADVSLLFDNVGLTPANTVPRMRSFPRNALTYAGICAALFPLAFLFVWRDA
                     ARRRWAWLAVACCLFGAAFSLARGAWVAIAIGLAYLFVDGSIDRRRKTQMVVAFVSAA
                     IVITGVFFIKYRVNPVTGRAGGGESVATREDLYKDTLGILKGPLRYIVLGYGTEKPRT
                     ASGTVKEGSRYVPRAGTHSTYLNYLFRTGVPGLLGIVALYALAFLHVRTRTRDSDDTE
                     RDLSTLAGMAVVIAAAHAVILSLYVEPIYTLTISLLLGIALASASEKSTLLPRPATQ"
     gene            12165..13718
                     /locus_tag="VNP73_08315"
     CDS             12165..13718
                     /locus_tag="VNP73_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWL65963.1"
                     /translation="MTDDPLLAEGLTQEAARDVGTVAKGGAIQIVGQMSERGLGFLFH
                     AATLQLLGPGGFGLLRQVEQLLTIAGQLGLTGFNYAVMRFITLARAAKQPGGVRGAAQ
                     VGMAGALIGSAIVVIGLLTGTDLVSERFADPGDVAAFSRLLWIGAAYVPLFGILQVLR
                     YSTQAYRTMVPSVVAGSIVKPVTRTVIGVGLVLAGFSVAGAVVSLVISAAIGTVVAGW
                     FFLRMITAEERAATPKRAVGPMIRFALPQGGSSLFGIQSLGLGILLLGALNTDAEVAF
                     FAVGLGLQGPGNIFLGGIVNIWAPVVSDLHARGDIARLDSLYKTINRWVATFSFPVFA
                     AMIIEADLFIRTFFPKAFPGAVAVAIILAAGNLFYVGTGPTGYVISMTGRPGVNFANS
                     VVGVVLYLILGIIFVPKYGAIGMAIVDSSVTVVINSARVVQAKVLVGVQPFGRSYIKP
                     VAATLAGSAVTFAFSFFADEKWPLEVTGIALGGIVFVLVLRVMGLDPEEQHVWQRIKE
                     RAFKLKRRG"
     gene            13670..14671
                     /locus_tag="VNP73_08320"
     CDS             13670..14671
                     /locus_tag="VNP73_08320"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWL65964.1"
                     /translation="MAAHQGASVQAEAARMTMSTRKEIRKSLSTNFDPGPRHYANRLM
                     LRMRRAGHLPALWRGGALDRPIFIIGAPRSGTSILFSVLRSSSAVAHWPGEAHEVWEA
                     DHHPALLGWRSNELGVDDLDAGTADRIRREFFLVTGSRKRLIDKTPRNVLRVPFIDRI
                     FPDARFVFLTRDGRDNVNSLINAWRSPRYRTYKLPKRHAIPGVDPEWWKFVLYPGWQD
                     DTKGPLERVCARQWVASNASVLDAEAASQPERWTRIRYEDLVDAPRAEVERVLRVLDL
                     PPEDAVLAKAEALDRTPINVVTPPEKGKWRRENPTEIGNVITLIAPTMERLGYSIED"
     gene            14688..>14945
                     /locus_tag="VNP73_08325"
     CDS             14688..>14945
                     /locus_tag="VNP73_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HWL65965.1"
                     /translation="MSDRTFLDITRDAQEFAHAAGTDGLLNAFAPHATAGLALMETAS
                     GSEEDLLKAVDRLLPRDQDYVHSHGSPGHGADHLLPAFVSPS"
BASE COUNT         2559 a         4663 c         4897 g         2826 t
ORIGIN      
        1 acaagagcgc cgcggccgac ttcctcgccc agcgggcgcg gggtggacct gcgctcgagc
       61 tgggcatcgg aaccgggcgg gtcgcgcttc tgttaaagga gcggggcgtc gccgtccacg
      121 ggatcgatgc ttcagagaag atggtcgaac gtcttcgcga gaagccgggt gggggcgaca
      181 tccccgtcac cgtgggcgat ctcgccgacg tcggtgtaga tggttcctat ccgttgatct
      241 acgttccgtt caacaccctg ttcgcactcg aatcgcagga ggatcagatc cgctgtttca
      301 agaacgtgcg cgaccgcttg accgatgacg gcgtgttcgt gaccgagacg ttcgttccgg
      361 acgtcactcg ttacgagcgc aaccaaggca cgtcggtgga ggacatccgt tccgatcgcg
      421 ttttcgtcag cttctcccag cacgaccccg taacgcagac ctcgatgacg cagcacgtgt
      481 tcttctcgtc gaagggcatc gagctcttcc ccgtgttcct tcgctacgcg tatccggctg
      541 agatggacct gatggctcgg gtcgccggcc tcgctttgcg cgagcgttat ggagactggc
      601 ggatggctcc gttcaactct tccagcaccg cacatatctc cgtctacggt ccggagtgac
      661 tcaagcgttc aggtatgggt gctttggacg aggaaccttc acgacgtcct ctgcttgcac
      721 cacgacctca ttgttcaggg gaaagaggtt gccggtgact tggacccact gatcctgctt
      781 gaactcgccg gccggagcat cgacgatccg gacctgaaca ctcaacgcat cggcaacgca
      841 gcacgagatc aggaagcgcg tcagaaggaa ctcgtcggcc ggcatcccgg ggtcgcggct
      901 gacgaagccc acgaactcga cctcggcccc cgcccgtgcg gtcagggctg ccatcgcttc
      961 atcggacctc acggctccgg ccacgtcggc aagtgacagg gagccggtcg agatgtcttc
     1021 gacgctggtc acgaatgcgg ccgaggcaaa gctcgacctg cggctggctg cgaacgagct
     1081 cagcgaggcc ggcggcagcg cgatgacgac gaccaccggt agcgcgacca atgctagtcc
     1141 ggacacggat cggttcgaga gggccggcga gtctggacgg gtgacgagat agccgacgac
     1201 cgagacgatc aggatgatcg ctccgacggg aaccaaccac gccgtgcggg cagaaaggta
     1261 gagcgacgtc cgatccgtcg cgagtaagaa gacatacaag cccgcccacg cgatcagcac
     1321 gaacagaacc gcgatcgcgg acggtgcccc tccgcgcctc atccgacgat cacctcgacc
     1381 cacagtgttc ctgcgaagac cacggcaccg acgaccagca cgaccgttct aaggaactca
     1441 cgagagaagg tcccggagta caggaacgcc agtttcgtat cgacgagcgg cccgaacacc
     1501 aggaatgcca gttgcgcgcc gacgccgaac tgcacgaagg acgccgctac gaaggcatcg
     1561 gattcggaac agagggatag gacgaacgcc agcgccatca tagcgatcaa gctgagaaag
     1621 ggcgtgtccg cgatcgatgc gatccaggtc tgggggacga acgtttgcat cgttgccgct
     1681 accgccgcgc cgagcacgag gaaacgaccc atgaagatga aatcaccggt caggtgtgaa
     1741 aagaacgatc cggcgcgact gtccgcgtga tgggcgtgct cgtcatcctc ggccgtggca
     1801 ctcaatagct cgccggaagt cttgttcgca acgatccacc ccaccgcgag cgcggcgagc
     1861 aggccgagag tcgcgcgacc tacaacgatc ggcacgagga tgtctcgtcc gcgataggcg
     1921 atgatcgtgg agacgagaac aaccgggttc aagatcgggg ccgcaagcat gaacgtgacg
     1981 gcggcgccgg cggaaaggcc cttgtgcgcg agtctgcggg cgaccgggac ggacccacat
     2041 tcgcagaccg ggaacgcgaa cccggtgagc ccggcggcgg ggagttgcac ggcgcgcggc
     2101 aagcttgcga gccgatcgaa aaccgagcgg gggacgaata cttcgatcac ggcggacacg
     2161 accgccccga gaaggatgaa cggcatggct tcgatcaaca aggacgagaa gaccaacagg
     2221 aaattctgaa gggcggggat ccgggccggg ttcaacaggt tgaggaccac ggctatccca
     2281 gccagagctg ccactgcgac gaaggaccgc cgccatgcag atctggtgtc gatcggggtc
     2341 aatgcagctt gggccatcaa cagaaccttg ccagagctac ggcgaaaccg gggaacgcgt
     2401 ccgcgaagac cggctccccg ccgatctccg tcgagcgcag cgccgcgatc acctcttgcc
     2461 gggagatctc ctcttgtgcc agtgcgtcct gatcgaggaa gaatcctcct gccacggacg
     2521 cctcgacaac gtcggcatcc agcctggtgc cgatctggtc cttgacgagc gggacgaatc
     2581 ctggtgacac cggtgtatcc ggatccgacg tgccgccgac cgggagcgag gtaagtacga
     2641 aggctgccga ccctccgctc gcgtcgttcg cggcgcgctc gatatcggcc agcgccttgc
     2701 cgaggtactg cttggtgccc ggctcaacgg ttccgctaag tacgacggcg aagagatcgg
     2761 gaacttcgtc tcggccgaag ccggtgcgaa gggcgagtgg aaaggattcg gccaccaacg
     2821 tggcattgtg gccgccgccc gaatactcga cgtagtcctc gtctacatcg atgtaccact
     2881 cgcccccgat cagcccacgg tcttgcggtt tccgacccaa gagtccgatc agggaacgct
     2941 gcctcgtgtc ttcgtcgaga tcatcggcga gtgtggcgat gacggtcccc gggcccaccg
     3001 gacctaccgg aggcgcgttc catgcgtgcc cgacccgtcc gtcatcgtcg cgtacgagcg
     3061 atcccgtgat gccgtgctcg accgggagac cgcccgttcc gatcgtggcc atgatggcgg
     3121 ccgggtcgag tgggagagag gcaacggcgc cgtcgaaagt gccggctccg tcgcgcgaac
     3181 gctcgcggac gaatcgtgct gttgcaagat cttccgggct tgcgccttcc aggatcacgt
     3241 aaacgaacaa acgaggagga tcccccgacg cgacgccctc aaccgggcga cccgctcgca
     3301 cctcggggtg agggatgtcg aacccgatga tctccgccgc ggtgggcgag atgtcttcga
     3361 tcccggccgt gatcgaaccc tgggtggcga caccgtggcc gtagaagacg atcggtacga
     3421 gatccgggcc gtcgccgagt gcgggccaga cggtgttgtc cctcgcaacc tgtgagctgc
     3481 tgcccgcagt caggaccacc gtcggctccg tcacggccag tacatcggga gaccggccgt
     3541 ccctgtagcc tgctgcgatc cgatcgcgaa cggacgcggg caacgagcag aaggggacct
     3601 gagcggctcg tgtccgctct tcctcgcagg cacggccgag gcacagcacg cgcaggaccg
     3661 cggccgggat ggcgatcaac gcaaggaggg ccagaatcga aacgacccgt cggtccgggt
     3721 gcacgttgat gacgctaccg tgtggcccct tatgagagat gccggcaatc gtttgtggat
     3781 cgtcatcggg gtcgctgtcg tggctgcagc tctggcggga tatcaattgc tgcccgaacg
     3841 aggtgtgcct gcaggtgcgc cgacgctcga cgagatggcc tcaggtttgg gtggtccgat
     3901 catggaacac ctcaaccggg gtcacgttcc gggacgctcc ggtgagttac tgctcgttcc
     3961 gaagcctcat cactacatca tcggcaagtg ggatctcacg acgctcggca ccgacgcgcc
     4021 gacgttgacg aactcgcatc ccaatccctg ggcttacatc gctcgcgtcc ccatcgttct
     4081 ctacgggccg ccgttcgttg ccgaagaccg tcgcgtcgaa cgctcggtgg atatcgcaga
     4141 tatggcgccg acctatgccg cgctgctcgg tatgcaggcg ctggacgccg acggtgaggt
     4201 gcttaccgag gcggttgaag actcacccga ccgtgcaccg aaggtgatct tcaccatcgt
     4261 gctcgatggg ggcggatgga acgttcttca ggagcatccg aactcgtggc cgacgatcca
     4321 atcgttgcgc gaggggggca ccgactacat caacgcgacg atcggatctg ctccgtcgat
     4381 caccggcgca ttgcatgcca ctttcggtac gggtgtctat cccgtgactc atgggattcc
     4441 cggtaaccag atgcgggggc cggacgggtt gaacaccgat gcgtggaaac agaacgccga
     4501 tccggagtac ctgctcgtac caaccgtgtc ggagttgtgg gacgagcaga acgctaacga
     4561 gccgatcgtc ggaacggttt cctacgaagg ctggcatctc ggcatgatcg ggcacggtgc
     4621 gcaacgcgaa ggtggtgaca aggacatcgc ggtgctgtgg gaagcggaaa ccaacgagtg
     4681 gtggatcaac gatgacttct acgaacttcc cgactacctg gccgagaccg acctggacac
     4741 tctcgaacgc tacgaagagg agctcgacga acgggacggg cttcccgatg gtctgtggtt
     4801 cggtcacgac ctcgaagagc tgcgtgatcc ggtcgttcgg cccggctcct cagctttcgc
     4861 ccgcttcacc ggggatgcgg tcgtcgacgt gatgcggcgc gagaagctcg gtgtcgacga
     4921 cgtccccgac atgttctggg tcgagatgaa gatgcccgat ttcgccggcc atcgctggaa
     4981 catgatcggg ccggaggaag ccgacgtact caaggtcgtc gacgatcaga tcgctcgctt
     5041 caagagggag ctcgaccgcc tcgccgggcc cggcaactac gtgatcgcga tcagcgccga
     5101 ccacggccag caaccgttgc cggagctcga ggggggctgg aggatcaact cgaacgagtt
     5161 gctggcggac atcgaggctc gcttcgggaa tgtcgtcgaa aagatcacga ccgtcgacat
     5221 tttcttcgag ccggaagcga tggagaacgg cgatgtcgac ctcgaggatg tggcgcgttt
     5281 cctgggtacc tatacggtcg gtgacaacat cccgcgcggt gccgtcggcg ccggcttcgt
     5341 gccggtggaa cgtctcgatg acacgatctt cgccggcgcg tttcccacga actacctagc
     5401 gtcgcccccg atggaattca cggaacccag ctgtgagtac ccaacggaat cgaatctgtt
     5461 cggtcccccc gggcccggct caacatgtgc ttcggtgatt gagtgatgtt gaaacgcggc
     5521 ctgcaactcc tcgtccagaa gcgcgatttc gggctcttga tgttggtcca gttcgcggcg
     5581 caagcgggcg acgcgatcat ccagacgtcg ctggcgaagt tcatcgtgtt cggtggcaat
     5641 aaaggcctcg acgtcgaagc tgctccatcg ccggacgtgc tgctgagcat ggtgctgttc
     5701 ctgttcgtcc cctacacgat catcagcccg ttcctcggag tcgtgatcga tcgctgggat
     5761 cgacggagat tgttgttcgg tatcaacgct ctgcgcgcgg cgggggcgat cctcgtcggg
     5821 ttcatcggga tcgaacgggt ggggaatctc actctgctga tcgtcttctt gctgaccctg
     5881 gctgccaccc ggatcgtcct ggcgaccaaa gcagcggccc tgcccaccgc gctcggcgat
     5941 aaggatctgg tcgaaggcaa tgccgtctct cagctcggcg gggcactctt ccagctgggc
     6001 ggaggtggtg tcgctctcgt ggccaccctg ctacttccgg ccgagcccgt tgccgtgacg
     6061 ggaggcatcg tctgcgccgt agctgcggcg atcgcgctcc ggatccggca tgccggcgag
     6121 actcgagagt catcgacgtt gggtcgtgag atcgcacgcg tgctgtccag catctgggcc
     6181 ggtctcagag aggttgcgcg gacgcccaag gcgggggccg cgatcaccac atacttctgg
     6241 ctgcgcttcc tgtggagtct ctcgatagta ggtatcggtt tcatcgcgaa ggaactcgtg
     6301 gcaggcaacg acaacgttcg cctggttctc accgcggggg gaggagcggc gggggccgtt
     6361 ctcggtttcc ttttggcaga caagctgcgc gcgcgcgtgc cgaacaccgc ccgcatcgtc
     6421 ctcctctctt ccgcactcgc agggttgggc acggccgcgc tgggggcgct ggagatcaaa
     6481 gcggcgatcg tcatgctcac gttcttccta ggcctggggt tcttcctggg gaaaatctcg
     6541 ctcgatacgc tggtgcagga ggcactcggc gatgacttcc gcggccgcgc gttctcgctt
     6601 tacgacatcg cctacaacgt tgcctgggtc atggcggccg tgatcatgaa gatctggtgg
     6661 accgatctcc acggcgttct catcgcgacc atgggcgtcg tcttcctgat cggcttggcc
     6721 gcgctcgcga tgtggtaccg ggccgcgggt ctgctcgcgc tccaacgtag ctagcgcgtg
     6781 tcgcgccgcc tgttctccgg tagtcgcctt ccgatcctgg gagcgggtct ggccctggtg
     6841 accgttgtgc tggccatgac cggagtcttg tcaccggccc ccgaagcccg cagcttcgaa
     6901 tctcagttct gtgggctgcc ggggccgtgg cgcgaacgca tccggcgcgg gtacttcggc
     6961 ccgcgctccg gacagatagc gatcctcccg cgctatccgg cctacagcgg caccagcgag
     7021 ggcgggtgga gtcactccgg cccgtggggt tatctccaac gcgttcctct ggtgttctac
     7081 gggcccgggg tgatccccgc agaaggtgac gtggagcgcc cggtgaccgt cgccgatata
     7141 gcgccctcct tgatgcgttt gatgagaggc acgctcaaca cacgcgactc ggatgcgctg
     7201 agcgaggctc tcccgcgtgg acccgcgctt gtaggcaatc cgtcaccccg gctgatcgtc
     7261 gtcagtgtgt gggacggcgg tgggtggaac gttctcgagc gctggcccga agcgtggcca
     7321 aatctgcgca gactgatgga cgagggaacg tccttcacga acgcgacggt tgggtcctcg
     7381 ccttctgtaa ctccatcggt gcacacgacg atcggaacgg gagtgttccc ctggcaccac
     7441 gggatcaccg ggatccccgt ccgcgacgag aacggagagg tggtcgattc cttcctgaag
     7501 ggagactcgt cgcgcttcat ccaggctcct gccgtggcgg aactgtggga cgagcatcac
     7561 gacaatcggg ctgagatagc gatggtcggc tacgagccgt ggcacctcgg catgatcggt
     7621 ctgggggcgg aagaccgaac gggggacaag gacgtagccg tctggctcga cacggagacg
     7681 aacgagtgga tcacgaatcc cgctcactat tcgaaacccg acgtcttcct cgatcgaacg
     7741 gtcctggatg aagagtcgcg cgctctagac gcggtggacg gagaggtcga ccggtcgtgg
     7801 agatccctcg acctcgacga tcccgcgcgc atcgaggaga cccctgcatt catcgcgtat
     7861 cacggtcacg tcatgagcga gttcatcgag caggaggggt acggacgcga ccgcatcaca
     7921 gacctgctgt tcactaactt caagcagatc gatcgcctcg gtcattactt caacatggct
     7981 tcggaggaag tcaggcaggc ccttgaggaa tccgaccggc agctcggcga gctcgtccga
     8041 gaactcgaca cgctcgttgg ccggggccgg tgggctgtag tcgtgacggc ggatcacggg
     8101 caacaacccg acgcgagagc tgtggatgga tacgggatcg accccaaaga ggtcgctcgc
     8161 gacgtggatg ccgagttcgg acccatcacc cgggcggtat ggccgaccga ggtcttcttg
     8221 cacgacgacg tgatgagtcg tgagggggtg accctcgaag aggtcagccg cttcctcagt
     8281 gattaccgca tggccgagaa cacccagcgg cccgacatgc tcgttggggg ggcgggaagg
     8341 ttcggccccc gggacacgct cttcgagctg gcgatgccct cgcggctgat ccccgggctc
     8401 gcctgcggac gccgatagga tgcgccatcg catgagggcc gatagcgcgg agacgagttc
     8461 tgccgccacc ggtgccccca aggatccgat ctcccgagcg ctcggacacc cggtggccta
     8521 cgtggctact gcagtgatcc ttctggccgt ttttgcgtgg ccgttcctgt ccgacgccgc
     8581 ccgcgtcgcc ccgacgaagg acccggcctt ctacacctgg aggaccgaag ccttcctcac
     8641 cgaggatccc gcgaccctcc tggggatcga gggaccgcaa gggatgttcg cggccgggta
     8701 tcgggttacc gctccgatcc tgggtgggct ccttcgccac gtcgcgggga tcggctcgtt
     8761 gcacgtcacc gttttcttga tggtcagtct cccggtcctg atcgcgctgg cattggccgg
     8821 tttcgcacac tcgcggttcc gggatcctct cgtgtggcac tccaccgcga tcggttccgc
     8881 cggactcttg ttgacacccc cgttcgttgg ttatctggac aacgtcctgt gtctgttctt
     8941 cctcgcggcg tctctcttct tcatcgaagg cgcccaacgg tcgtgggtcg atcgcgcact
     9001 gtttttcgtc cttctacttc tagcggggtt gacccatccg acgacgctgg tgatcttcgt
     9061 tgccacgctc gggctgatgg ccggcttaaa gctgctcctg tcgaagttcg acatcaaagc
     9121 caccttggtg tccgacggtt ggatgctcgc gaccggagcc gcttcggtgg ttgccacggt
     9181 cgcgatctgg acctttgggc tgtgggggcc ttccgtgtca ttgaccgaag cggcgttgcc
     9241 acctccgtac ggcgcgagtt tcttcatcga caggatgatg ctgtggatcg gcacgatgcg
     9301 ccccgttctc aacggccccc tcttcctcgt gggccttgtc ggactgctgg ccgggggccg
     9361 cgaactgctc gcgagcacac atgtgcgtgc gatcctgtcc tggcttgcac ctcttgccgg
     9421 tttgttcggc ttcatcgccg gtttgacgta tccctattac aggttcttca acacgacatt
     9481 gtcgtgggtg ttgctcgtgg ggatcggcgg cggtttcgcc gcgcgcttct tcctgtcggg
     9541 tggaagaacc ggcgcggcgc gggtcgcgat gtctctgggc gctgtcgccg tcggggtgat
     9601 cctcgtgacc aacatcacgc atggattgga tgtgtcgggc tggacgaagg tgaagaacca
     9661 atggctcgag cccgaagcac gcgccgacct cgacgagttg cgtcaagccc tggcgctcga
     9721 agaccccgat cgtccggtgg tgttcgtgat cgacgacgaa ctgccggagc cgttccagat
     9781 ctacggcgcc agcaagttgt acggcaacac ttcccgctac gggcttcctc ccggccagat
     9841 cgatcgcggc tacctgtacc tcggcagcat cgagaacctg atcgcgggag aaccgacgtc
     9901 gaccggagat gagacctacg acccgctgtc gaccgcgttg ctggaggaca ccacccaggg
     9961 gatccagaga tccggcctcg atcccgtgat cgtgatcgcc aagatcttca acccggccgg
    10021 cgcgaacgcg caggtcgcgg ccggcgagga ggacctgccg gagtccgtca cctcgcagct
    10081 gacaagtcag gtaggtgtgg ggcggtcggt gtggacattg cacgatgggt cgatctacga
    10141 gtggacggac gcaggccgcg tcgggttgga acgctccggc ctccaacccg aggacccgaa
    10201 cgcgatcgtg aacctccttc aggtcctctt cggtcttgtt ctcatagcgc ttcccggcct
    10261 cgcttggtac ctgtggtcga acgatgatcg ggccaccccg agggcgctgg cactcgtccc
    10321 ggccgtcggt atgggtgtgg tgaccctcgg cggcgtcgtc gtcgttgcga tcgcgcgggc
    10381 tccgttctcc gctcctgtcg ctttcgccac catcgcggtt ggactcgtcc tcgccgttgc
    10441 gatgcgcttc cttccacgac acgaagtcgc gtgatcgcag cgttgctgga acgcttgccg
    10501 acggaacgcc ccggccgcaa cggccgcatc ttgtgggtct taaccctgat agcgggcgcc
    10561 gcggggatcg ccggatgggc gacccagacc ctggtggggg gagtggtggc tctgtggagc
    10621 ctggccgcgg ctgcagccct cgtggcgatg gtcctgccgt tccggtacgc gctggtttct
    10681 ccgttgtaca tgggggtcgc cgggtggctc gtggacatgt tgccgttcgt gatcctgatc
    10741 ggatggggag tggtgtcgct gaggtggggc atcaaggtcc tgcgcgaacg ccggtggcca
    10801 cgagggggca ggctcatctg gatcccgatc gggttggtct tctggaccgc tctcggcgtc
    10861 gtggtgatca cgcggctcga cttcagacat ttccttcttc tcttggggat ccaagtcatc
    10921 gcgtcggcgg ccgtgttggc cacgctcgac tcggtgcggg gcgtgagcga acggatggat
    10981 ctggtccgag cactccttgg gttcgtggtc cttctcaccg tcgcggtatt cgcacagtgg
    11041 atcgggatcg acatcgagac actgcagaac ggtgccacgc gcaagcgtgt ggaagcggcc
    11101 tacggcgtcg acgcgttccc caacaacatc ggcatgatca agtacgcccg ctcgatcaac
    11161 gcgggcgcac tcgagaagca gcgcgagatg gagcgggtcc aagcccgcac ggccggctta
    11221 ccggacttcg aagtgtttcg acccaagttc caggcgtacg agaacagcct cgtggtgagg
    11281 ttcgaaggct cggcgcggcc ctacgagcag gctctgggcg aggccgacgt gtcgctgctg
    11341 ttcgacaacg tgggactcac gccggccaac accgttccgc ggatgcggtc gttcccgcgc
    11401 aacgcattga cgtacgcggg catctgcgca gctctcttcc cattagcgtt cctgttcgtg
    11461 tggcgcgatg cggctcgacg ccggtgggca tggctggcag tggcgtgttg cttgttcggc
    11521 gctgctttct ctctggcgag gggtgcgtgg gtcgccatcg cgatcggcct tgcctacctg
    11581 ttcgtcgacg gatcgatcga caggaggcgg aagacacaga tggtggtcgc cttcgtttct
    11641 gctgcgatcg tgatcaccgg cgtgttcttc atcaagtatc gggttaaccc ggtgacggga
    11701 cgggccggtg gaggtgagag cgttgcgacg cgagaggatc tctacaagga cacactcggg
    11761 atcttgaagg gtccccttcg ctacatcgtc ctcgggtacg gaacggagaa gccgcgcacg
    11821 gccagtggga cggtgaaaga aggatcgcgt tacgtgccac gcgcgggcac gcactcgacc
    11881 tatctcaact atctgttccg aacgggcgtg ccgggtctac ttgggatcgt cgccctctac
    11941 gcgcttgcat tcctacacgt gcgcactagg acgagagact ccgacgacac cgaaagagac
    12001 ctctcgacac ttgccgggat ggcagtggtg atcgcggctg cacacgcggt gatcctgagc
    12061 ttgtacgtcg agccgatcta cacgctgacg atctcgttgt tactcgggat cgcgctcgcg
    12121 tcggcgtccg agaagagcac cctgctgcct cggccggcga cgcaatgacg gacgatccgc
    12181 tccttgccga aggcttaacg caggaggccg cgcgggacgt cggcacggtc gccaagggcg
    12241 gagcgatcca gatcgtcggg cagatgtccg agcggggact ggggttcctc ttccatgccg
    12301 cgacccttca gctgctcggt cccgggggct tcggactcct taggcaggtc gaacagctgc
    12361 tgacgatcgc gggtcagctc ggcctgacgg gcttcaacta cgccgtcatg cgattcatca
    12421 cccttgcgcg cgcggccaag caaccgggcg gcgtgcgcgg cgcggcccag gtggggatgg
    12481 caggggcact catcggctcc gcgatcgtgg tgatcggtct gcttacgggc accgacctcg
    12541 tgtccgaacg gttcgcagat cccggcgacg tcgccgcctt cagccggctc ttgtggatcg
    12601 gagccgcgta cgtgccgctt ttcgggatcc ttcaggtcct ccgttactcg acgcaggctt
    12661 acagaacgat ggtgccgtcc gtcgtcgccg gatcgatcgt gaagccggtg accaggaccg
    12721 tgatcggggt cggcctcgtg ctcgcaggct tctcggtcgc aggtgcggtg gtgtcgttgg
    12781 tgatcagcgc ggcgatcggg acggttgtcg cgggctggtt cttcctccgc atgatcacgg
    12841 ccgaggagcg tgccgctaca ccgaaacggg ccgtgggccc gatgatccgc ttcgcattgc
    12901 cgcagggggg atcgtccctg ttcggcatcc agagcctcgg cctcggcatc ctgctgctcg
    12961 gtgcactgaa caccgatgcc gaggtcgcct tcttcgccgt cggcctgggc ttgcaaggtc
    13021 cggggaacat cttcctcggg ggcatcgtga atatctgggc accggttgtg tccgatctcc
    13081 acgcgcgcgg cgacatcgcg cggctcgact ctctctacaa aacgatcaac cggtgggtgg
    13141 cgacgttctc gttccccgtg ttcgctgcga tgattatcga ggccgacctc ttcatccgaa
    13201 cgttcttccc caaggcgttc cccggcgcag tcgcggttgc gatcatcctt gcagccggca
    13261 atctctttta cgtgggcacc ggcccaaccg gttacgtgat ctcgatgacc ggtcgcccgg
    13321 gcgtcaattt cgccaactcg gtcgtggggg tcgtgctcta cctcatcctc ggaatcatct
    13381 tcgttcccaa gtacggcgcc atcgggatgg cgatcgtgga ttcatccgta acggtagtga
    13441 tcaactccgc ccgcgtcgtg caggcgaagg tgctggtggg agtgcagccg ttcggtcgct
    13501 cctacataaa gccggttgcc gcgacactcg ccggatccgc ggttacgttc gccttctcct
    13561 tcttcgcaga cgagaagtgg ccgctggagg tgaccgggat cgcgctggga ggcatcgtct
    13621 tcgtactcgt gctgcgggtg atgggcctcg atcccgaaga gcaacacgtg tggcagcgca
    13681 tcaaggagcg agcgttcaag ctgaagcggc gcggatgacg atgtcgaccc gtaaagagat
    13741 acggaagagc ctgtcgacga acttcgatcc cggcccccgg cattacgcga acaggctgat
    13801 gctgcgcatg cgccgggccg ggcatctccc tgctctgtgg cggggcgggg cgctcgaccg
    13861 cccgatcttc atcatcggtg ccccgaggtc gggaacgtcg atcctgttct cagtcctgcg
    13921 ctcgtcgagc gcggtggccc actggccggg tgaagcccac gaggtgtggg aggccgatca
    13981 ccatccggct ctcctcggat ggagatcgaa cgagctcggc gtggacgacc tcgacgcggg
    14041 gacggccgat cgcatccggc gtgagttctt cctggtgacc ggttcacgca agagattgat
    14101 cgacaagacc ccgcgcaatg tcctgcgggt tccgttcatc gatcgcatct ttccggatgc
    14161 ccgtttcgtg tttcttacgc gggatgggcg cgacaacgtg aactcgttga tcaacgcgtg
    14221 gaggtcgccc cgttaccgca cctacaagct gccgaagcgg cacgcgatcc ccggggtcga
    14281 tcccgaatgg tggaagttcg ttctttaccc cggctggcag gacgacacca aaggtcctct
    14341 cgagcgtgtg tgtgcgcgcc agtgggtcgc ctcgaacgcg tccgtcctcg atgcagaggc
    14401 tgcatcgcaa cccgagcgct ggacccgcat ccgttacgag gatctcgtcg atgcaccccg
    14461 ggcggaggtc gagcgggtgc tccgtgtcct cgaccttccg cccgaggacg ccgtgctggc
    14521 gaaggcggag gcgttggacc ggactcccat caacgtggtg acgccccccg agaagggcaa
    14581 gtggcggcga gagaatccga ccgagatagg aaacgtgatt actctgatcg ctccgacgat
    14641 ggaaaggctc ggttactcga ttgaagactg aggtgcgttt ctacgacatg tccgatcgga
    14701 cgtttctaga catcacccga gatgcccagg agttcgccca cgccgccggc acggatggat
    14761 tgctcaatgc cttcgctccg cacgccactg ccgggcttgc tttgatggaa acggcgtcgg
    14821 ggtcggaaga agatctgcta aaggccgtcg acagactgct gccgagagac caggattacg
    14881 tccacagtca tgggtcaccc ggtcatggag ccgatcatct attgccggcg ttcgtctctc
    14941 catca
//
LOCUS       DATMED010000322         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1803327, whole genome shotgun sequence.
ACCESSION   DATMED010000322 DATMED010000000
VERSION     DATMED010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803327"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VNP73_08330"
     CDS             <1..515
                     /locus_tag="VNP73_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HWL65966.1"
                     /translation="DGTDAVMLSEETAIGDYPVESLRVMSEIAVEAEQRTMASARSAS
                     HEDDPVSWAIARAAVQASEELDVAGILCPTRSGATARRVAAFRPRMPILATDHTAHVL
                     RSLSIVWGVVPFEVPFLSDDQLAAHGLERALTAARDSGVAVSGDLITMVAGGAAPRAG
                     STDLMRVLHL"
     gene            complement(504..1559)
                     /locus_tag="VNP73_08335"
     CDS             complement(504..1559)
                     /locus_tag="VNP73_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255838048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1221..1223),aa:Sec)
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
                     /protein_id="HWL65967.1"
                     /translation="MLLTSPLVSAAEFERRTGWAIKPEGACKGDRCVPLRDTSTDKFD
                     ISELAKALRMPLVRDEKHDLWAVGPEADAPPLASARVPHFTLPDRNGDEFTLDSLRGQ
                     KVLLIAWASWUGCRFDLPGWQALRDDLHPKGLELVTVALDTGGVDAAGPWIDAANPEH
                     PSLIDQAHILDELFGVVNVPTGIWIDEDGVVVRPPETAFPLKRGMEKLLRTLPEGLDP
                     YLVDVLVEVKKVKYKPDKYIGALKDWVDKGRDSRFALSPEEVTSRTGSRSMEASTAAA
                     HFEIGQHLHRNGNAADAIGHFRKAHELQPENWTYKRQAWTFVNPMQGPTIEHYDTDFL
                     SEVRKVGAENYYPALEM"
     gene            complement(1578..>2115)
                     /locus_tag="VNP73_08340"
     CDS             complement(1578..>2115)
                     /locus_tag="VNP73_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65968.1"
                     /translation="SERADELLLLAPDHEPPAGFESKVLAQVGGKERRRGRTYLVAAA
                     AALIVGIASAGGVMLASAEDREIASHYKEALAEANGDYFGVKPLMVPNGSKVGNVFSY
                     GGETDWMFVVFNEGMGPGVYQVDIRTESRGTVGAGVITLGGNERTWGADLTVELKDVE
                     ALVFTERSGDVFEADFSH"
BASE COUNT          352 a          677 c          667 g          419 t
ORIGIN      
        1 tcgacggaac cgacgccgtg atgctctcgg aggagactgc gatcggtgac tatccggtcg
       61 agagtttgcg agtgatgagc gagatcgcag tggaagccga gcaacgaacg atggcttcgg
      121 cccgcagcgc ttcccatgaa gacgacccgg tgtcgtgggc catcgctcga gctgcggttc
      181 aagcttcgga agaactggat gttgccggga tcctgtgccc aacccggagc ggggcgacgg
      241 ctcggcgcgt ggccgccttc cggccccgca tgccgatctt ggcgaccgac cacacggcgc
      301 acgtgctccg ctcactttcg atcgtgtggg gggtggtgcc gtttgaagtg ccgttcctgt
      361 cggacgatca gttggccgcg catggactcg agcgcgctct aaccgcagct cgcgactcgg
      421 gggttgccgt gtcgggggac ctgataacga tggtcgccgg aggagctgca ccgagagcgg
      481 gcagcaccga cctgatgcga gtcctacatc tctaacgcgg ggtagtagtt ctcggccccg
      541 accttgcgca cctcactgag aaagtccgtg tcgtagtgct cgatggttgg gccctgcatc
      601 gggttgacga acgtccacgc ttgtcgcttg taggtccaat tctcgggctg cagttcgtga
      661 gcctttcgga aatgcccgat cgcatcggcc gcgttcccgt tgcgatgcaa gtgctggccg
      721 atctcgaaat gggcggcggc tgtcgaggct tccatcgaac gcgatcccgt tcgtgacgtg
      781 acctcctcgg gactcagcgc gaacctgctg tctctgccct tgtcgaccca gtccttcagc
      841 gcgccgatgt acttatccgg cttgtatttg accttcttta cttcgaccaa cacgtccacg
      901 aggtaagggt cgagtccttc cggcagcgtg cggaggagtt tctccatgcc ccgtttcagc
      961 gggaaagccg tctcgggggg ccggacgacg accccgtctt cgtcgatcca gatcccggtc
     1021 ggcacattca cgaccccgaa gagctcgtcg agtatgtgtg cttgatcgat cagtgatggg
     1081 tgctcggggt tggctgcgtc gatccacgga cccgcagcgt ccacgccacc ggtatcgaga
     1141 gcgaccgtga ctagttcgag gcccttcgga tgaaggtcgt cgcgcagtgc ctgccacccg
     1201 ggcaggtcaa aacggcaccc tcaccacgac gcccatgcga tgagtagaac cttctgtccg
     1261 cgcaggctgt cgagggtgaa ctcgtcgccg ttccgatcgg gcaacgtgaa atgggggacc
     1321 cgcgcggacg cgagtggcgg cgcgtctgcc tccggaccca cggcccacag atcgtgtttc
     1381 tcgtcgcgta cgagtggcat ccgcagagcc ttcgcaagct ccgagatgtc gaacttgtct
     1441 gtagaggtgt cacgcagggg aacgcaccgg tcgcccttgc acgcgccttc gggcttgatg
     1501 gcccatcccg ttcttcgttc gaattccgcg gccgaaacga ggggacttgt gaggagcatc
     1561 gtgattcgac tctaggccta atgcgagaaa tctgcctcga agacgtcgcc gcttcgctcg
     1621 gtgaagacga gggcctcaac atctttgagc tcgaccgtga gatcggcccc ccaggtccgc
     1681 tcgttcccac ccagggtgat aacaccggcc ccgaccgtgc cccggctttc ggtccttatg
     1741 tcgacctggt agacgccggg ccccatcccc tcgttgaaga cgacgaacat ccagtcggtc
     1801 tcgcctccgt atgagaagac atttccgacc ttgcttccgt tcggaaccat caggggcttc
     1861 acgccgaagt agtcgccgtt cgcttctgca agcgcttctt tgtagtggga cgcgatctca
     1921 cggtcctcgg cgctggccaa catgacgccg ccggccgacg cgatgcccac gatcaaggcg
     1981 gcagccgcgg ccacgaggta ggtccgaccg cgtctacgtt ccttgccccc gacctgggcc
     2041 agcaccttgc tctcgaaccc agctggcggt tcgtggtcgg gggcgaggag caacagctca
     2101 tcggcgcgct cggac
//
LOCUS       DATMED010000323         7403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5639739, whole genome shotgun sequence.
ACCESSION   DATMED010000323 DATMED010000000
VERSION     DATMED010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5639739"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VNP73_08345"
     CDS             complement(<1..667)
                     /locus_tag="VNP73_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HWL65969.1"
                     /translation="MFVLLALVVTMPGLSPVPGSASSHREAPLIAADPQADNTDLYAY
                     VSPDAPDTVTLISSWIPFEEPAGGPNFYAFGEGVRYDINVDNNHDAKPDVIYRWTFQN
                     HYRSGETFLYNTGPVTSLGDENLNFYQTYDLKRVTPGSGQTTLVNDAVAVPSHTGDPS
                     MPDYGALRNEGIRSFGDGGKAWAGQADDPFFLDLRVFDLLYGGDLSEAGDDTLAGFNV
                     NALA"
     gene            complement(859..2316)
                     /locus_tag="VNP73_08350"
     CDS             complement(859..2316)
                     /locus_tag="VNP73_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65970.1"
                     /translation="MSGVVRRGLFAGVLVALVAAGLPVQTAAAAVGVSSFNPNVGGLV
                     SIAFDPESDNLFIYPAHAAEILEFTTSGSQVGSGIPTPRASNDVDLDFALEPLTVAGV
                     SVPTNTLLAADGETSPVNLYALDKDDGSIASTQTMPGTTSVGGAQHPARDSFMQVSYG
                     EHTIHEFDPADGELIGSIPVAPEGSPPFSMYFGDIDVDLDTGNIVVVSSNQNVIRAVS
                     PTGEWLGDVDVYDENDQSDPSNVFGMSGVAIDDDTNTVYVTGTGGQVWILTGLLESFS
                     TSSEPAENPCETDPDAICGDEGDNDIQGTEEDDTIYAGEGNDTIDGGGGNDVIVGGAG
                     NDTLTGGGADDQLVGGEGDDVLVGDDVTGYPKIQGRRMFGPFLQEDPPPPGNDTLDGG
                     AGNDFVDGSLGNDTVKGGAGVDTVVGGAGKDNVTGQAGGDDLAGSGGPDVLNGGGGTD
                     DMNGGPGKDTCVLDTRKEQSRTPSCERKKLNFKRN"
     gene            complement(2432..3763)
                     /locus_tag="VNP73_08355"
     CDS             complement(2432..3763)
                     /locus_tag="VNP73_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL65971.1"
                     /translation="MRWSVRARATASAVAIVGTALICAAVSIVLTIHRSSEENIRSAA
                     RLRVQDFVALLESGNDPSDLDIRFEPGVFVQVVDESGRVVAASPQLEGLPPAAQIGTY
                     ESATVDNPRGGREPFLVVSDYALTESEDLRVLVGRSVDVVAEATRVLITALAIAVPLL
                     LAIVGVATWMMVGRSLAPVEAIRAEVAAISGSELHRRVPDPPGDDEVARLARTMNHML
                     ERLEEAQLKQRRLVADASHEFRNPLAAIRQHAELALAHPGQATAAELAGDVLPESLRL
                     QHIAEDLLLLARADEDALALKIAPLDMKRLVLEEAARLESVTSMTVDTSGVEEAEIAG
                     DSEQLHRLVRNLTDNAARYASSTVGLALRAFDGHVLLHVDDDGPGIPVDRRRSVFERF
                     SRLDEARGRRMGGAGLGLAIVPEIVGANGGSVAVSDAPLGGARFEITLPRK"
     gene            complement(3778..4455)
                     /locus_tag="VNP73_08360"
     CDS             complement(3778..4455)
                     /locus_tag="VNP73_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL65972.1"
                     /translation="MRILVVEDEKGLAAGLKKGLEAEGFAVDVALDGTDGLWMAREQS
                     YDAIVLDIMLPGVNGFRICSTLRDEGNWTPIMMLTAKDGELDHTEALDSGADDYLTKP
                     FSYPVLVAHIRALLRRGRGERPTVLEAGDLKLDPAARRCWRGEEEIELTAREFSLLES
                     LFRRKGDVVSKREIMEHVWDFDFEGDPNIVEVYIRHLRDKIDRRFDRASIQTVRGFGY
                     RLDGSGG"
     gene            complement(4530..6461)
                     /locus_tag="VNP73_08365"
     CDS             complement(4530..6461)
                     /locus_tag="VNP73_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor C family protein"
                     /protein_id="HWL65973.1"
                     /translation="MGPRTIKRPWLAAAAATCLFVGMGISAQAAEGVVGNGGFETPAH
                     ESRWQVYTPGDAEMAPWTIGSGDVEQISDLYWVPAEGTMSLDLNGRGPASIYQDIDTV
                     AGKTYTLTFSLSANPGDPQGLKTMDLYWGGQKASSFSFDSTGTDYQDMRWEQKSIRLT
                     ADTALTRLEFRSTTSSDSHGPALDDISLSGGAAVQPQGIGVSSFNPGLGSLIAIAFDH
                     EVNNLFIYPEVNDQVFEYSTSGSQVGAGIPVPGARSNDFDMDFATESLSVGGDSVPAN
                     TLLAANNDAGNLVFAVDKDDGAIEAQQALPAGTAATGGAQHPARDSYFVVSWSEHLIK
                     EVDPDDGALLNSFPVQPEGSPVFSTNYSDIDVDNETGNILVLSSNQNVIRLLSPAGEW
                     LGDADVFNESDTEDPLRISAMSGVAIDDATKDVWITTTNGIVYKLTNLWENFSTAVPC
                     ESDPNALCGDDGDDEIQGTPEDDTIYAGEGDDTVNGGGGNDVIIGAAGNDTITGGDGN
                     DVIVGDDAGKGTLPVLHSRALVAFLQEDTAGPPGNDILDGGGGNDQVKGGGGTDTVAG
                     GAGKDRVTGQAGGDDLSGSAGPDFLNGGNGVDGMNGGGGKDTCVLDNKKERSRTASCE
                     KKKLNFKLNFLPLRFLRSP"
     gene            complement(6461..>7403)
                     /locus_tag="VNP73_08370"
     CDS             complement(6461..>7403)
                     /locus_tag="VNP73_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWL65974.1"
                     /translation="LYALSQGLSFYPAASGGTGFTANAWKQVVVTRDSSDNVTGYLDG
                     TQVLTFVDSSNYGVVANDLLQFFVDNQTGNAANREQSAGAVARMRFYDDALSSSEVSG
                     LDRLPSAAPVSGESSTPVCGSETNDTIQGSEEDDTIYAGEGDDTINGGGGDDVIIGAG
                     GNDVINGGDGDDVIIGDEESDTPEAAAGYPLLGAFLQEEPPAPPGNDTLDGGLGNDVV
                     DGDAGDDTVKGGGGADSVEGGEGKDKVTGQAGADTLSGSAGPDNLNGGGGVDGMNGGG
                     GKDTCVLDSKKERSRTASCERKKLNFKRSLPSRFLWI"
BASE COUNT         1332 a         2384 c         2187 g         1500 t
ORIGIN      
        1 gtgccagagc gttcacattg aacccggcta acgtgtcgtc gccggcctcg gacagatcgc
       61 cgccgtagag aaggtcgaac acgcggagat cgaggaagaa cggatcgtcg gcctgaccgg
      121 cccacgcctt tcctccgtca ccgaacgaac ggatcccttc gttacggagc gcgccatagt
      181 ccggcatcga tggatcgccg gtatggctgg ggaccgcaac tgcgtcgttc acgagagtcg
      241 tctgtccgct gccgggcgtg acgcgcttca ggtcgtaggt ctgatagaag ttcaggttct
      301 cgtctccaag cgaggtcacc gggccggtgt tgtagaggaa cgtctcgccc gacctgtagt
      361 ggttctggaa cgtccaccga tagatgacgt ccggtttcgc atcgtggttg ttgtcgacgt
      421 tgatgtcgta gcgaacgccc tcaccgaacg cgtagaagtt cggcccaccc gcaggctctt
      481 cgaacgggat ccacgagctg ataagggtga ccgtgtccgg tgcatccgga ctcacgtaag
      541 cgtagagatc ggtgttgtcc gcctgaggat cggccgcgat caatggtgct tcccggtgac
      601 tggatgcgct ccccggcacc ggcgacaaac ccggcatcgt caccaccagc gccagcagaa
      661 cgaacatccc tacgaacagc gtccgtctct tcctcatctg agctcggact cggacagcga
      721 aggtcattgc gccctcccct tacctcgagc cggctctttt gcgccggccg ttcgacaggg
      781 tttcgcaacg acgcgtcggc gcggattgga cgcgcgcaca acgtccggtt tgttcgttgt
      841 gtccttacaa gtggtagatc agttcctctt gaagttgagc ttcttcctct cgcacgacgg
      901 ggtgcggctc tgctctttcc tggtgtccag cacacaagtg tctttgccgg gcccaccgtt
      961 catgtcgtcg gtaccgccgc caccgttcaa tacatcggga ccaccactac cggcgaggtc
     1021 gtcgcctccg gcttggccgg tcacgttgtc cttaccggct ccccccacga ccgtatcgac
     1081 acccgcgccg cccttcaccg tgtcgttgcc gagtgatcca tcaacgaagt cattcccggc
     1141 accgccgtca agggtgtcgt tgcccggtgg aggcgggtcc tcctggagga aaggaccaaa
     1201 catgcgtcgc ccctgaatct tcggataacc ggtcacgtca tcaccgacga ggacgtcatc
     1261 accttcgccc cccacgagct ggtcgtccgc gccgcctccg gtaagcgtgt cgttaccggc
     1321 acctccaacg atcacatcgt taccaccgcc gccatcgatg gtgtcgttgc cttcgccggc
     1381 gtagatcgtg tcgtcttcct cagttccctg gatgtcgttg tcgccctcat cgccacagat
     1441 cgcgtccgga tcggtctcgc acggattttc cgccggctcg ctgctcgtgc tgaacgactc
     1501 gaggagcccg gtgagtatcc acacctgtcc gccggttccc gtgacgtaga cggtgttcgt
     1561 atcgtcgtcg atcgcgactc cggacatgcc aaaaacattg ctgggatcgc tctgatcgtt
     1621 ctcgtcgtag acgtccacgt ctccgagcca ctcgcccgtc ggcgacaccg cgcggatcac
     1681 gttctggtta ctgctgacca caacgatgtt tcccgtgtcc agatcaacgt cgatgtctcc
     1741 gaagtacatg ctgaagggag gggatccctc gggagcaaca gggatgctgc cgatgagttc
     1801 gccatcggcg ggatcgaact cgtggatcgt gtgttcgccg taagacacct gcatgaacga
     1861 gtcgcgagcg ggatgttgag ctccaccaac cgacgtggta cccggcatcg tctgagtgct
     1921 cgcgatggag ccatcatctt tgtctagcgc atacaagttg acagggctcg tctctccgtc
     1981 ggcggccaac aaggtgttcg ttggaaccga gacaccagcc actgtcagcg gctcgagtgc
     2041 gaagtcgaga tcgacgtcgt tgctcgcgcg tggagtgggg atcccgcttc cgacctggga
     2101 gcccgaagtg gtgaactcga ggatctccgc agcgtgagcg ggatagatga acaggttgtc
     2161 cgattcgggg tcgaatgcga tcgataccaa accgccgacg ttcgggttga aagaactaac
     2221 gccgactgcg gccgcagccg tctgaacggg caggcccgca gccaccaacg ccacgagaac
     2281 ccccgcgaac aggccacgcc taacgactcc ggacatatcg gcccccatct gatcggttga
     2341 attaccaatc tatggatggg ggccgagtcg gcccatagct agtccggact atttatcccg
     2401 taaggcggtg tccggttccc gacagcttgc tctacttacg agggagcgtg atctcgaacc
     2461 tggcaccgcc cagcggtgcg tcgcttacgg cgacggagcc tccgttggca cctacgatct
     2521 ccggaacgat cgctagaccc aatcccgccc cacccatgcg gcggccgcgc gcttcgtcga
     2581 gccggctgaa gcgttcgaag acggagcggc gcctgtcgac ggggatcccc ggtccgtcgt
     2641 cgtccacgtg caacagaacg tgtccatcga aggcacgcaa agccagtccc accgtcgacg
     2701 aggcatagcg tgcggcgttg tctgtcaggt tgcgaacaag cctatgcagc tgctcggagt
     2761 cgcccgcgat ctctgcctct tcgacccccg acgtgtcgac cgtcatggac gtgacgctct
     2821 caagacgagc ggcttcctcg agcaccaacc gcttcatgtc gagcggcgcg atcttcagcg
     2881 ccagcgcatc ttcgtccgca cgagccagta ggaggagatc ctcggcgatg tgctgcaagc
     2941 gaaggctctc gggaagaacg tccccggcaa gctcggctgc ggtcgcctgg ccggggtggg
     3001 caagcgccag ctcggcgtgt tgtctgatcg cagccagagg gttcctgaac tcgtgagatg
     3061 catccgcgac gagtctcctc tgcttgagtt gcgcttcctc caatcgctcg agcatgtgat
     3121 tcatcgtgcg cgcgagacga gcgacctcat cgtcgccggg gggatcgggc acgcgtcggt
     3181 gcaactcgga gcccgagatg gccgccactt ctgcgcggat cgcttccacc ggcgcaagag
     3241 agcggccgac catcatccat gtagctacac caactatcgc cagtagaagg ggtacggcga
     3301 tggctagagc cgtaatcagg acgcgcgtcg cctcggctac cacgtcaaca ctcctgccga
     3361 ccaagaccct cagatcctcg gactcggtca gcgcatagtc cgacaccacc aggaatggtt
     3421 ctctgccccc ccgggggttg tcgaccgttg cgctctcgta cgtcccgatc tgagccgccg
     3481 gcgggagtcc ctcgagttgg gggctcgctg cgacaaccct tccgctttcg tcgacgacct
     3541 gaacgaatac accgggctcg aatcggatgt cgagatcgct cggatcgttt ccgctttcga
     3601 ggagagccac gaagtcctgc actctcaggc gtgcagccga tctgatgttc tcttctgaac
     3661 tgcggtggat ggtgagaacg atagagactg cggcgcagat aagagccgtg cccacgatcg
     3721 cgacggccga agcggtcgcg cgggcacgca cggaccaccg cactaggtca ccgaccccta
     3781 cccaccgctg ccgtccagcc ggtaaccgaa cccacggacg gtttggatcg atgcacggtc
     3841 gaagcgccga tcgatcttgt ctcgtaggtg gcggatatag acctcgacga tgttgggatc
     3901 gccttcgaaa tcgaaatccc aaacatgttc catgatctcc cgcttggaga cgacgtctcc
     3961 cttacggcgg aacagcgatt cgagtaacga aaattcccga gcagtgagct ctatctcctc
     4021 ttcgcctcgc cagcatcggc gcgcggcggg atccagcttg agatcaccgg cttcgagaac
     4081 ggtgggccgt tcgccccggc ctcgtcgcag tagtgccctg atatgggcca cgagaacggg
     4141 gtacgagaac ggcttcgtca ggtagtcgtc ggctccactg tcaagtgcct cggtgtgatc
     4201 gagctcaccg tctttcgcag tgagcatcat gatcggggtc cagttgccct cgtcccggag
     4261 tgtcgagcag atacggaaac cgttaacgcc gggaagcatg atgtcgagca cgatcgcgtc
     4321 gtaggactgc tcgcgagcca tccagaggcc gtcggtaccg tccagagcca cgtcgaccgc
     4381 gaacccttcg gcttccaggc ccttcttcag accagcggcc aggcctttct cgtcctccac
     4441 gacaagaatg cgcactgagg gcctcctcga tcaccaagcc ttacgcatct cgccatgttg
     4501 ctgaacacag gctgaaagcc ggctgaaaac tagggactgc ggagaaacct caggggcagg
     4561 aagttgagct tgaagttgag cttcttcttc tcgcaggaag cggtacggct gcgctccttc
     4621 ttgttatcaa gcacgcaggt gtccttgccg ccgcctccgt tcatgccgtc gacgccattg
     4681 cctccgttga ggaaatcggg gccggcgctg ccggagagat cgtcgccgcc ggcctgcccg
     4741 gtgaccctgt ccttgccggc accacccgcg acggtgtcgg tgccaccccc gcctttgacc
     4801 tggtcgttgc cgccacctcc atccaggatg tcgttacccg gtggtcctgc ggtgtcctcc
     4861 tgcaggaagg caacgagggc tcgagaatgg agaaccggaa gcgttccctt acccgcgtcg
     4921 tctccgacga tcacgtcgtt cccatcgccg ccggtgatcg tgtcgttccc ggcggcaccg
     4981 atgatgacgt cgttcccgcc gcccccattg accgtgtcgt ccccttcacc ggcgtagatc
     5041 gtgtcgtctt ccggcgtccc ctggatctcg tcgtccccgt cgtctccaca caacgcattt
     5101 gggtccgatt cacatggaac ggcggtcgag aagttctccc acagattggt gagcttgtac
     5161 acgatgccat tcgttgtagt tatccagaca tccttggtcg cgtcgtcgat ggcgaccccc
     5221 gacatcgcgg agatcctcaa cggatcctcg gtatcgcttt cgttgaagac atctgcatct
     5281 ccaagccact ctccagcagg agacagaagt cggatcacgt tttggttgct gctcagtacg
     5341 aggatgttgc cggtctcgtt atccacgtcg atatcgctgt agttggtact gaaaacggga
     5401 gagccctcgg gctgcaccgg gaagctgttc aggagtgccc catcgtccgg atcgacctct
     5461 ttgatcaggt gctcggacca cgaaacgacg aagtaggagt ctcgagcggg atgttgtgct
     5521 ccgcccgtgg ctgcggtccc ggccggcagc gcctgttgag cttcgatcgc gccgtcgtct
     5581 ttgtctacgg cgaagacaag gttgcccgcg tcgttattgg cggccagaag agtgttggcc
     5641 ggtacggagt cgcccccgac cgaaagcgat tcggtggcaa agtccatatc gaagtcgttg
     5701 cttcgagctc ccggcacagg gatccccgct ccaacctggg agccagaagt gctgtactca
     5761 aacacctggt cgttcacttc tggatagata aagaggttgt tcacctcgtg atcgaaggcg
     5821 atggcaatca aggagccaag gccggggttg aaggaactta ctccgatccc ctgcggctga
     5881 acggcggcgc cgccgctaag tgaaatgtcg tcgagagcag gaccatggct atcggaagaa
     5941 gtcgtactcc tgaactcgag tctcgttagt gccgtgtcgg cagtgagccg aatgcttttc
     6001 tgctcccatc gcatgtcctg gtagtcggtc ccggtcgaat cgaagctgaa ggacgaggcc
     6061 ttctgcccgc cccagtacag atccatcgtt ttgagcccct gtgggtcgcc ggggttggcg
     6121 gataaagaga aggtcagggt gtacgtcttt cctgccacgg tatctatgtc ttgatagata
     6181 gacgcgggcc cacgaccgtt gagatcgagc gacatagttc cctcggccgg aacccagtag
     6241 agatctgata tctgctcgac gtcacccgag ccgatcgtcc acggcgccat ctctgcgtct
     6301 ccgggtgtat agacctgcca tcgggactcg tgcgcggggg tttcgaatcc gccgttgccg
     6361 acaacgcctt cggccgcctg ggcgctgatc cccattccaa cgaacaaaca ggtggccgcc
     6421 gcggcggcca gccatggtcg cttgatcgtc cttggtccca ctagatccaa aggaatcggg
     6481 acggcagcga gcgtttgaag ttgagcttct tgcgctcgca cgacgcggtg cggctccgct
     6541 ccttcttcga atccagaacg catgtgtctt tgcccccgcc accattcatc ccgtctacgc
     6601 cgccaccgcc attcagattg tcggggccgg cgctacccga gagggtgtct gctccggcct
     6661 ggccagtgac cttgtctttt ccttcccctc cttcgaccga gtcggcaccg ccgccaccct
     6721 tgaccgtgtc gtcaccggcg tccccgtcca ccacgtcgtt tccgagccca ccatcgagcg
     6781 tgtcgttccc gggaggagcg ggcggttctt cctgaaggaa cgccccgagc aacggataac
     6841 cggcggccgc ctcgggggtg tcgctctctt cgtcaccgat gatcacgtca tcgccgtcac
     6901 caccattgat gacgtcgttg ccaccggctc cgatgatcac gtcatcgccg ccgccaccgt
     6961 tgatcgtgtc gtcgccttcc ccggcataga tcgtgtcgtc ttcctcagac ccttggatcg
     7021 tgtcgttggt ctccgagccg cagacggggg tcgaagactc tccggagacg ggagctgcgc
     7081 taggaagtcg gtcgagtccc gatacctcgc tagaactcag agcgtcgtcg tagaagcgca
     7141 tgcgcgcaac cgctccggcc gactgttcgc ggttcgccgc gtttcccgtc tgattgtcga
     7201 cgaagaattg caggagatcg ttcgcgacca ccccgtagtt gctcgagtcg acgaaggtga
     7261 gcacctgcgt cccgtcgaga taaccggtca cattgtcgga agaatcgcgc gtgaccacca
     7321 cttgcttcca tgcgttcgct gtgaaacccg tacccccact cgcggccgga tagaaagaaa
     7381 ggccttgact gagggcgtag aga
//
LOCUS       DATMED010000324         3470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1416582, whole genome shotgun sequence.
ACCESSION   DATMED010000324 DATMED010000000
VERSION     DATMED010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416582"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VNP73_08375"
     CDS             complement(<1..204)
                     /locus_tag="VNP73_08375"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810132.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWL65975.1"
                     /translation="MANSVIVSAARTAIGKFGGAVSTVPATDLGARAIKGALERAGVS
                     GEQIDYVIMGQVLQAGTGQITARQ"
     gene            334..1122
                     /locus_tag="VNP73_08380"
     CDS             334..1122
                     /locus_tag="VNP73_08380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWL65976.1"
                     /translation="MEDVGLFGPQSMVWRVHSDPASLIGGLRGLLIQALNPVAMAAVS
                     QHSDYRDDPWGRLNRTSKYLTITTFGDTRRAHEAATRLRSIHRRISGVDPVTGRSYRA
                     DDPDLLLWVHNVEVHSFLAGYRAYGGCVTTAQADQYVSEMVRHANLVGLDADDVPHTY
                     AEVDHFVSNAQLALTPAAREGMRYVLSPPMPIPLRPLWAIPAAAAVAILPGHIRDLYG
                     IPWVAPATPVVRTSTTAVLKAWKALFPPPPPVREALDRAARLAA"
     gene            1383..1541
                     /locus_tag="VNP73_08385"
     CDS             1383..1541
                     /locus_tag="VNP73_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65977.1"
                     /translation="MKDRFTDGNSDDVFVTHLKWSTNFPNEGRGAYTVEWRQNGGRTG
                     KRLGFHRN"
     gene            1569..1967
                     /gene="mce"
                     /locus_tag="VNP73_08390"
     CDS             1569..1967
                     /gene="mce"
                     /locus_tag="VNP73_08390"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWL65978.1"
                     /translation="MLNKIEHVALAVDDLEAAVAHYSDVWGLEVSHRERVEDQGVEEV
                     MLQLGDSYLQLLGATDADTTVGKFLERRGEGLHHIAYEVDDLEATLASLKAKGVEMID
                     DEPRLGGRGHLVAFVHPKSNHGLLVELIQR"
     gene            2022..3314
                     /locus_tag="VNP73_08395"
     CDS             2022..3314
                     /locus_tag="VNP73_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL65979.1"
                     /translation="MQHSDNAARKAPSPLSNPAFRKLLAISVTVALGYGLVVPILPNF
                     ARSFDVSLAAVGLVFFVFGLTRFLFGLVGGLVVDRFGDRFSVMAGLLIVAASSYASGL
                     STTFPQLVAARGFGGAGSALFIAGLMNRIIRTIEPEAMGRATGQFRSSFLVGISVGPL
                     LGGILAKSFGDAATFHVYGSGLLIAAVIAYFVMAGQAVDRTAPRSSPLESLRKAAPLL
                     RDRRYVLALAATFVGWWVLAGPAQTIGPVFASEELGFSKFAIGAAVTLLAIGEVLVLF
                     AAGSAADRLGRKAVLVPSLVVTAVAIALVGQIEEAPWAYFPLMVAAGAGVAAGSTAAG
                     GLLADAIRGHGAQQKGGSGSAVGVNQMAGDLGYLLAPTTVGFLAELGGFGLAFLAAAV
                     PAALLLAWTVTLPNYRAERSGDADRPPEAVPEPGPVAP"
     gene            complement(3239..>3470)
                     /locus_tag="VNP73_08400"
     CDS             complement(3239..>3470)
                     /locus_tag="VNP73_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65980.1"
                     /translation="FSAGVASLDPTDTPGGFLTRADEALYLAKHRGRNRVEHDPTPEA
                     IAGNKDPSYGATGPGSGTASGGRSASPDLSAR"
BASE COUNT          588 a         1099 c         1111 g          672 t
ORIGIN      
        1 ttgacgcgcg gttatctgcc cggttcctgc ctgaagcacc tggcccatga tcacgtagtc
       61 gatctgttcg cccgagaccc ccgcgcgttc gagcgcaccc ttgatcgcgc gagcaccgag
      121 atcggttgcg ggaaccgtcg aaacggcgcc accgaacttt cctatcgccg tacgagcggc
      181 cgagacgatt acggaattgg ccattggcgc tcctttgggg acgcggggtg agccttggtt
      241 gaagcttcag tctaagggcc gcgaccgttt tctttgtgcc tgagtgcccg tctggcggga
      301 cctctcgcgc tcagaatcgg tgagtatggg gcgatggagg acgtggggct attcggtcca
      361 cagagcatgg tgtggcgggt gcactccgat ccggcttctc tgatcggagg acttcgggga
      421 cttctcattc aggcgctgaa ccccgtagcc atggcggccg tctctcaaca cagcgactat
      481 cgagacgatc cctgggggcg gctgaatcga acttccaagt acctgacgat caccaccttc
      541 ggtgacaccc gccgcgcgca cgaagccgcg acgagactgc gttcgatcca ccggcgaatc
      601 agcggtgtcg atccggtcac gggacggagc tacagagccg acgatcccga tctgttgctc
      661 tgggttcaca acgtcgaggt ccattcgttt ctcgccggtt atcgggctta cgggggctgt
      721 gtcacaacgg ctcaagccga ccagtacgtg agcgaaatgg ttcggcacgc aaatctcgtc
      781 gggctggacg ccgacgatgt gccccacacc tacgcagagg tcgaccactt cgtcagcaac
      841 gcacagcttg cgttgactcc ggcggcgcga gaggggatgc gctacgtgct ttcgcctccg
      901 atgcctattc cgctgaggcc tctatgggcc attccagcgg ccgccgcggt ggcgatactg
      961 cccggacata ttcgcgacct ttacgggatc ccgtgggtgg cacccgccac gccggtcgta
     1021 agaacttcga caaccgcagt cctaaaggcg tggaaggcac tctttcctcc acccccgccg
     1081 gtgcgcgaag ctttggaccg cgcggcccgc ctggccgcgt agggcaaatc acgtatcgga
     1141 gggagctcct catgaaaagg gcatcaatca tcgtgtcgct tagcgcggct ctggttgtcg
     1201 gattgtttgc cggcggggcg ctggcgagcc atcgaccaaa caacttctgt tcccaaacgg
     1261 gggactactg tcaatccaca gccaggaaca acaagggggt tcgcgtcctc cagtttcgaa
     1321 gctttgctca tcgaggaaag gtaagggttt gcgtcaaggc acccaccaat accaggacgt
     1381 gcgtgaagga tcgcttcacc gatggcaaca gcgacgacgt cttcgtcacc catctgaagt
     1441 ggagcacgaa cttccccaac gagggacgcg gcgcgtacac ggtcgaatgg cggcaaaacg
     1501 gcgggaggac tggaaagagg ctgggcttcc accgaaactg atcaaccggt cttgcataag
     1561 atgcgtcgat gctcaacaag atcgaacacg tcgcccttgc agtcgatgac ctcgaggctg
     1621 ccgtagccca ctactccgac gtgtgggggc tcgaggtgtc ccaccgagaa cgcgtcgagg
     1681 atcagggtgt cgaagaggtc atgctccagc tcggagattc gtaccttcag ttgctggggg
     1741 caaccgacgc cgatacgact gtcggtaagt tcctcgagcg ccgaggggag ggactgcacc
     1801 acatcgctta cgaggtcgac gacctcgagg caacgctcgc ctcgctcaag gccaaaggcg
     1861 ttgaaatgat cgacgacgaa cctcggttgg ggggaagggg acacctggtc gccttcgtcc
     1921 atccgaagtc gaaccacggc ctcctcgtcg aactgatcca gcgctagcgg tcgcccggcg
     1981 gcggcggaca attactttga gcatctagag aaaaggacca cgtgcaacac tccgacaacg
     2041 cggcgcgcaa ggccccctca cccctgagca atccggcgtt tcgcaagctg ttggcgatct
     2101 cggtaaccgt tgccctcggc tatgggttgg tcgttccgat tctgccgaac ttcgcgcgga
     2161 gctttgacgt cagccttgcc gcggtcggtc tcgtgttctt tgtgttcggc ctcacccgct
     2221 tcctcttcgg actcgtcggg ggcctagtcg tggatcggtt cggtgaccgc ttcagcgtca
     2281 tggccgggct gttgattgtc gcggcgtcat cctatgcatc cggcctgtcg acgacctttc
     2341 ctcagctcgt cgccgcccga ggtttcggcg gagcgggctc cgcactcttc atcgcgggtc
     2401 tcatgaaccg aatcatccga acgatcgagc ctgaggcgat gggcagagcc actgggcagt
     2461 tccgctcctc gttcctcgtt ggcatcagcg tcggtcctct cctcggtggg atcctcgcca
     2521 agtccttcgg tgacgccgcg acgttccatg tctacggctc tgggcttctg atcgccgcgg
     2581 tgatcgcgta cttcgtcatg gcggggcagg cggtcgatcg aacggccccg cgctctagcc
     2641 ctctcgaatc gctcaggaag gcggcaccgt tgctgcgcga ccgccgctac gtcctcgcac
     2701 tggcagcgac cttcgtcggt tggtgggtgc ttgccggacc cgcccagacc atcggtcccg
     2761 tcttcgcttc agaggagctc ggcttcagca agtttgcaat cggcgcggcg gtgacgctgc
     2821 tcgccatcgg cgaggttctc gtcctcttcg cggccgggag cgcggccgac cgtttgggac
     2881 gaaaggcggt gctcgtgccc tcgcttgtcg tgaccgcagt ggccatcgcc ctcgtcggcc
     2941 agatagagga agctccctgg gcctactttc cgctcatggt cgcggcgggg gccggcgtgg
     3001 cggctgggag cacggccgcg ggagggctcc tggccgatgc gatcagaggt catggcgctc
     3061 agcagaaagg cgggtcggga tccgcggtgg gggtcaacca gatggccggc gaccttgggt
     3121 acctactggc gccgacgact gtcggtttcc tcgccgagtt gggcggcttc gggctggcct
     3181 tcctcgccgc agccgttccc gcggctctcc tgctggcgtg gaccgtgacg ctcccgaact
     3241 accgagcgga gaggtccggg gacgccgacc ggcccccgga agccgtgccg gagcccggcc
     3301 ccgtagctcc gtagcttgga tccttgttgc cggcaatagc ttcaggggtg ggatcatgct
     3361 cgacgcgatt ccggccccgg tgcttggcca gatacagggc ctcgtcggca cgagtgagaa
     3421 aacctcccgg cgtgtcggtg ggatccaacg aagcaacccc cgcggagaag
//
LOCUS       DATMED010000325         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5640649, whole genome shotgun sequence.
ACCESSION   DATMED010000325 DATMED010000000
VERSION     DATMED010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5640649"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VNP73_08405"
     CDS             complement(<1..598)
                     /locus_tag="VNP73_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL65981.1"
                     /translation="MGGTRVRALVHVSALVAASIAWTQPVLSQEEGGDRCGRRASIVG
                     TEGDDVLEGTPGHDNIAGLGGNDVIRGLDGSDSLCGGDGDDQVFGGKGGDHVEGSDGN
                     DSVDGGPGNDYIQGNLGNDALIGDAGFDTTVFSRSDSAQTIDLAAGTASGGMGDDTIA
                     EIEEVWGSTWGDTIIGSDGPDRIWLGEATDEVDGGGGDD"
     gene            complement(638..1606)
                     /locus_tag="VNP73_08410"
     CDS             complement(638..1606)
                     /locus_tag="VNP73_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWL65982.1"
                     /translation="MRAEIPQRNAPSWMVWTALWIVYIVWGSTYLAIRISVETLPPLL
                     AGGIRFLFAGALVYLFLLWRKGRDAVRVTKDELIGAGFVGTALLLGGNGIVMVAEQYV
                     ASGLASLLIATVPLWVILLRTLARDNVPRGTLGGVFAGFAGVALLVLPGDRPDGAPLG
                     GMLLLILAAAFWAPGTFYSRRVALPKDPFLSTSIQMLLGGLALVVSGLVRGELSGLRI
                     EEFSARSLIAITYLVVFGSLLAFTAYVWVLQHAPVSKVATYAYVNPVIAVFLGWAVLS
                     EELTAYVLGGAAIIVSSVAFIVRKESQPDAVDAPEMGAPAVASADA"
     gene            1703..>2202
                     /locus_tag="VNP73_08415"
     CDS             1703..>2202
                     /locus_tag="VNP73_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWL65983.1"
                     /translation="MQPSKLRSLALGLLMLLTACAGNEEPAADRTPEDPPSTSAESPD
                     AADEVEVEVRLPGIKGDEIPVEYTCEGNDTPPDIEWEGPVDASEWIVSVTDPDAPGGT
                     FVHWVVYRIPPGETELSGRLPPGAFEATSSFGNVGYGGPCPPEGDDPHGYVFTVTALS
                     EPLDLR"
BASE COUNT          451 a          750 c          658 g          343 t
ORIGIN      
        1 agtcgtcacc tccgccgccg tcgacctcgt cggttgcttc accgagccag atgcgatccg
       61 gaccgtcgct cccgatgatc gtgtcgcccc acgtcgaccc ccacacctct tcaatctccg
      121 cgatcgtgtc gtcccccatt ccaccgctgg cggtccccgc ggccagatcg atcgtttggg
      181 cggagtccga gcgagagaag accgtcgtgt cgaacccggc atcgccgatc aacgcgtcgt
      241 tgccgaggtt tccctggatg tagtcgttgc cgggcccgcc gtccaccgaa tcgttcccgt
      301 ccgatccttc gacgtggtcc ccgcccttgc cgccgaagac ctggtcgtca ccgtccccac
      361 cgcaaagcga gtcggatccg tcgagacctc ggatcacgtc gttgccgccg aggccggcga
      421 tgttgtcgtg ccccggtgtc ccctcgagca cgtcatcacc ttcggtaccg acgatcgatg
      481 cacgacgtcc gcagcgatcg ccgccttcct cttgggatag gaccggctgc gtccacgcga
      541 tggacgcagc cactaacgca gagacatgga ccagagcccg gacgcgggta ccccccatca
      601 accaagtatc caacgagcca cgcgttaggt gaaggggcta agcgtcggca gaagccacgg
      661 ccggtgcgcc catctcgggt gcatcgaccg catccggctg tgactccttg cgcacgatga
      721 atgcgaccga ggaaacgatg atcgcggccc cccccagtac gtaagccgtc agctcctctg
      781 aaaggacggc ccagccgagg aacactgcga tcaccgggtt gacgtacgcg taggtcgcga
      841 ctttcgatac cggcgcgtgc tgaagaaccc agacgtaagc agtgaaagcg agaagggaac
      901 cgaagacgac gagataagtg atcgcgatca acgaccgcgc cgagaactcc tcgatccgta
      961 gtccagaaag ttcgcctcga acgagaccgc tcaccaccaa cgccaaacca ccgagcagca
     1021 tctggatcga cgtggacagg aacggatctt tcgggagagc cacccggcgc gagtagaaag
     1081 tgcccggtgc ccagaacgcg gccgcaagga tcaggaggag catgccgcca agcggcgccc
     1141 catcgggtcg gtcccctggc aatacgagaa gggccacacc ggcgaaaccc gcgaacactc
     1201 cgcccaaggt tccgcgtgga acgttgtccc gcgcgagtgt tcgcagcagg atcacccaca
     1261 ggggaaccgt tgcaatcaga agcgacgcga gcccggaagc cacgtactgc tccgcgacca
     1321 tcacgatccc gttgccgccg agcaacagcg cagttcctac aaagccggca ccgatgagct
     1381 cgtccttcgt cacccgaacg gcgtctcttc ccttccgcca gaggaggaac agatagacga
     1441 gcgccccggc gaacaggaac cggatccccc ccgcaaggag tggcggcagg gtctcgacgc
     1501 ttatgcggat cgcgagatag gtcgaccccc acacgatgta gacgatccac agtgccgtcc
     1561 agaccatcca gctgggggcg ttcctctggg ggatttcggc tctcatacct agagccaact
     1621 agaggccgcg gcccgctatt tccgatcgcg cagattccga aggatgaaag gtccgagcgc
     1681 cctggatagt ctcgaaagga gcatgcaacc ttcgaaactt cggtcgctgg cgctcggttt
     1741 gctgatgctg ctcacagcat gcgccggaaa cgaagaaccg gcggcggacc gaacgcccga
     1801 ggatcctccg tcgacgagtg ccgaaagccc ggacgcagcc gacgaggttg aggtcgaggt
     1861 gcggcttcct gggatcaaag gtgacgagat ccccgtcgaa tacacctgcg aagggaacga
     1921 caccccaccg gacatcgaat gggaaggtcc ggtcgacgcc tccgaatgga tcgtgagcgt
     1981 caccgacccc gatgctcccg gcggaacgtt cgttcattgg gtcgtttaca ggatcccgcc
     2041 cggcgagacg gaactgtccg gacgtctgcc accgggggca ttcgaagcga cgagctcgtt
     2101 cggcaatgtc gggtacggcg ggccctgccc tcccgagggt gacgacccac acggatacgt
     2161 cttcaccgtc accgctctgt ccgagcccct cgatctacgc aa
//
LOCUS       DATMED010000326         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4110163, whole genome shotgun sequence.
ACCESSION   DATMED010000326 DATMED010000000
VERSION     DATMED010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4110163"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VNP73_08420"
     CDS             complement(<1..878)
                     /locus_tag="VNP73_08420"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWL65984.1"
                     /translation="MNLIAREAGWWTYSAEDAPVLGMPGELWFVWVVLWGVVAPLVTT
                     DRPMAVTIVGFLWLDLIFMPLFDGIVVLNASWVVGDLVAMAVALVPGLFLARWTERSE
                     RLFARIALQVLCSVGLFLWMIPSAALAQTGGWSEVLALPGWRLGLVAQATLLPIALGV
                     RAVIEFATRGDGTPVPYDPTKRLVTSGPYAFVRNPMQVAMTVLFVILGVGFRSPWMFF
                     AAVVAWAYSAGLAEWHEGAQLAERFGAEWSNYRGRVRAWIPRLKPVAGPSAILLIAYS
                     CGTCSSIGRWFVRRKPV"
     gene            complement(938..2140)
                     /locus_tag="VNP73_08425"
     CDS             complement(938..2140)
                     /locus_tag="VNP73_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65985.1"
                     /translation="METPERPKIPRPSRWLVMAWVSFAAVVWTAWLVASELDGGEGYY
                     ALAVKILGSAGILHLLFLVFRRPTSFEERPADEEADQAGLASEPEIESGARARWTLIS
                     LILVLVFGAVLYRLAQDSYLHSTSAFFIGVPSVIAIALTFAPRSGSATGLILRGITIA
                     MLLSGIVLGEGLICVLMASPLFYVVGIGIGQGIDRVQRDRGNEGRLYSIVGIAVLLMS
                     AEGVTPATTLPTQQSVTVTRTIDASTSSVRGALAKPPRFDAELPIYLKLGFPQPRRAM
                     GRGIEGGDGRVIFFGNESPMEPMGAGHHQGHHGQAPKGAITFRIAASEPGRIVFEVVD
                     DQTAFTHWVEWGRSIVEWHASGDDRTTVTWTVEFERKLSPAWYFGPWQRYAGRLAADY
                     LIDTAATP"
BASE COUNT          492 a          794 c          697 g          375 t
ORIGIN      
        1 acgggctttc tgcgtacgaa ccaacgtccg atcgagctgc acgtgccgca cgagtaggcg
       61 atcagcagaa tggcgctcgg gcccgccacc ggcttgaggc gcgggatcca ggcgcgcacc
      121 cgaccgcggt agttactcca ctcggctccg aagcgctcgg cgagctgtgc tccctcgtgc
      181 cattcagcca acccggccga gtaagcccac gcaaccacag ccgcgaagaa catccacggg
      241 ctccggaacc caacacccaa gatcacgaac agaacggtca tcgctacctg catcgggttg
      301 cgcacgaatg cgtagggacc gctcgtcacc agacgcttag tcggatcgta gggaacgggc
      361 gtgccgtcac cgcgcgtcgc gaactcgatc actgcacgca ccccgagcgc gatcggtaac
      421 agagtcgctt gggcgacgag cccgagtcgc caacccggca gggcaaggac ctcgctccac
      481 cccccggtct gcgctagcgc cgcgctcggg atcatccaca agaagagtcc caccgagcac
      541 agcacttgaa gtgcgatgcg cgcgaacagc ctctcagaac gttccgtcca acgcgctagg
      601 aagagccccg gcacgagcgc taccgccatc gcgacgaggt cgcccaccac ccacgatgcg
      661 ttcaggacca cgatgccatc gaacaggggc atgaagatca gatccagcca caggaacccg
      721 acgatcgtta cggccatagg tcgatctgtt gtgaccagag gcgccaccac accccacagc
      781 acgacccaaa cgaaccagag ttcgcccggc atgcccaaga ccggagcgtc ctctgccgag
      841 taagtccacc atccggcctc tctcgcgatc agattcacga ccgggagcgt ccatacgttc
      901 catatcgtcg ccaaaagcac ccctgtcatc cgcttgttca tggagtcgct gcggtgtcga
      961 tgaggtagtc agccgctagt cgtcccgcgt atcgctgcca tggaccgaaa taccaagccg
     1021 gggacaactt cctctcgaac tcgaccgtcc aggtgacggt tgtccgatcg tcgccggagg
     1081 cgtgccattc gacgatcgat cgcccccact cgacccagtg ggtgaacgct gtctggtcgt
     1141 cgacgacttc gaacacgatc cgaccgggct cgctggcggc gatgcggaac gtgatcgctc
     1201 ccttaggagc ctgaccatgg tgaccctgat ggtgtcctgc gcccatcggc tccatcggcg
     1261 actcgttgcc gaagaagatc acgcgcccat cccccccctc tatcccacgg cccatcgccc
     1321 tgcgcggttg cgggaagccc aacttcaggt agataggcaa ctcggcatcg aacctcggtg
     1381 gtttcgcgag agcacctcta actgaagacg tgctcgcatc gatcgtgcgc gtcaccgtga
     1441 cgctctgttg cgttggaagt gttgtcgccg gggttacgcc ttccgcgctc atcaagagaa
     1501 cggcgatgcc gacgatggag tacaggcggc cctcgttacc gcgatcccgt tgcactcggt
     1561 cgatcccctg accgatcccg atgccaacga catagaagag cggcgaggcc atcaggacgc
     1621 agatcagacc ctcgccgagg acgatgccgc tcaagagcat ggcgatcgtg atgccgcgca
     1681 ggatcaagcc ggtcgcactt ccggatcggg gtgcaaacgt cagagcgatc gcgatgacgg
     1741 atggcacgcc gatgaagaag gcggatgtgc tgtgcaggta cgagtcctgg gccaggcgat
     1801 aaagaacggc gccgaagacg aggacgagga tgagagagat cagcgtccag cgagctcggg
     1861 cccccgactc gatctccggc tcagaagcaa ggccggcctg gtcggcctcc tcgtcggccg
     1921 gcctctcctc gaacgacgtc ggccgccgga acacaaggaa caagaggtgc aggatcccgg
     1981 ccgagccgag gatcttgacg gccaacgcgt agtagccctc gccgccatct agctcgctcg
     2041 ctaccagcca cgccgtccac acgacggccg caaacgacac ccacgccatg accaaccatc
     2101 gagacgggcg agggatcttc gggcgttccg gtgtctccat cttttccccc tttggtcctt
     2161 cttaatcgaa tgattaaatc atatgattaa gaagtcggca aaggcaaggg agaacttgaa
     2221 tgggtcggga acaggctgag gaacggcggg cccagatgat ctcgaccgcg atcgaatcgc
     2281 tgaaggaggt gggctacgcc gggacgacga tccggtccct cgcccgccgc gggggcttca
     2341 actcggctct catctcct
//
LOCUS       DATMED010000327         5956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3722600, whole genome shotgun sequence.
ACCESSION   DATMED010000327 DATMED010000000
VERSION     DATMED010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722600"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VNP73_08430"
     CDS             <1..436
                     /locus_tag="VNP73_08430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL65986.1"
                     /translation="ELRELADAVTSKGVTPEIEFAGRLDHDPLSKLLPTFDLLVVPSV
                     VPEAFGMVAAEAAACGVLPLVPGHSGIGEVGATLEAALDRPGLLTFDPLDPVRDIARG
                     IDRVLALDPRDRRELGRRAVQVARELWSWDGVAARLLALATD"
     gene            446..1471
                     /locus_tag="VNP73_08435"
     CDS             446..1471
                     /locus_tag="VNP73_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HWL65987.1"
                     /translation="MSTFTYEGTENRMRWDRRRTGFMEVWYATLNHRPSGSGAWFRYT
                     LTAPDHSVGAPYCELWGFFFDPDGKRSFAAKKRFPIDHLGAPNGRDDGALVRIGDSWL
                     SETHLEGELASEQGSLRWSLDFEPADRCFQHLPEQLRTRVEDRVSTVCSPNLSVPFTG
                     TVEVGGEVFDFEAEPGCQSHRWGRRHSQTWSWGHCATFQEDPSAIFEGVAARANIGPV
                     PGPTTTFLYLHQGGEDLVFNELRWALRARSRYEMPTWAFTAKNDRWKIAGAARARLAD
                     LVQVEYADPDGSPRFCANSEIADLAIEVYERSGSTWRHHNSLTALGTGHVEFGRPQAF
                     SELPVSF"
     gene            complement(1454..1891)
                     /gene="arfB"
                     /locus_tag="VNP73_08440"
     CDS             complement(1454..1891)
                     /gene="arfB"
                     /locus_tag="VNP73_08440"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWL65988.1"
                     /translation="MKGIRVTRSLVIPDDEIKFTFSPSGGPGGQHANRSSTRAELVWN
                     VEDSRAVGPRQRDRIKAGLKRRIDANGNIRVASDRHRSQLRNREEAASRLAGLLAAAL
                     KPPKARVGTAPTKAAKERRLKAKRKRSDLKKNRRSVRDLEGNG"
     gene            complement(1888..3888)
                     /locus_tag="VNP73_08445"
     CDS             complement(1888..3888)
                     /locus_tag="VNP73_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWL65989.1"
                     /translation="MANRLAAETSPYLIQHKDNPVDWYPWGEEALARARDEDKPILLS
                     VGYAACHWCHVMEHESFEDPATAAIMNEHFVAVKVDREERPDIDAIYMEAVQTMTGQG
                     GWPMTVFLTPQGLPFYGGTYFPPEERYGMPSFSNVLHAITDAWQSRRDDVELQGKRLV
                     EHLNPIGRIEPSSDDVTRADLHQAFDKLRQNFDPDHGGFGGAPKFPQPMTLDLAIRLA
                     LRDRPDARTLYISTLDRMAAGGIFDQIGGGFHRYSVDRAWIVPHFEKMLYDNAQLLRT
                     YARAWAQDHNPRYAEIVALTAGWLLREMRDPEGGFWSSLDADSEGVEGKFYVWEPDEI
                     RALLGDESSAGEAYWGVTPEGNFEGKNILLATGAEPEPEVLGRWRETLMQARDQRVRP
                     GTDTKVITAWNALTAAAFAEAGTIFSRKEWIEVASEAMRFTFDGLVRDGRLMRSYREG
                     RAQHLGFCEDHAAVIEACLALFEATQDVEWLERARSTADAAITLFHDEAGPGFFTAGN
                     DAEQLVTRSKDVIDNAVPSANSVMALQLQKLTLLTGDNRYEDRSLEVMRLLREAAINS
                     PLAFAHLLEAVDFYTGNPREVVIVGAEGDHGTEALVETVRARWDPGQLLLVGNEGTVD
                     LPLFRDRKKADGLATAYVCHRGVCHAPVTTPEELARQLDADR"
     gene            3943..4233
                     /locus_tag="VNP73_08450"
     CDS             3943..4233
                     /locus_tag="VNP73_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65990.1"
                     /translation="MAALAYVLLPFSGLLCYLLGHSPRVRFHGLQAIALGALWPALLY
                     VAGGISEQATRLVALIGAIVWIGITIATAAGKDLHLPVLGARLRRVAEVERS"
     gene            complement(4230..>5956)
                     /locus_tag="VNP73_08455"
     CDS             complement(4230..>5956)
                     /locus_tag="VNP73_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65991.1"
                     /translation="EAALDAARLELERQRKTLSELRKESTEQKAALDELLKQREHEIS
                     ADSAQEERAKAEAALEDARRERSEEQAAVKAMWKERDEHRAATDTARRERAELEKSAA
                     EAAHEHDQEQASLETTRQEIERKRAARADLRKEADKERAALDELLKERDRETGLDDTR
                     QERAKAEADLEVARKDRATAQATLQTLIGDLATEESAIEQTLAEVTELQKQIKIARTR
                     QAAEKKAAETARTELTTAQSKLDKTSEDAAAASTELDALQTRLKQEKEQIEARIRKLA
                     DDKEALASDRTRRDAEETAARQRLEVEQASLKTAETELQNLTAETETARAEATRLSGE
                     IETAKAELQKELASIERLRQTREQERSSVASIQAERTELEAAIKEARSGRQREESATR
                     SAKKQLDKARRPPSKLFGRKADEAEAVEGRPFQKDGRWWYRRDGELLVFEEQTGEWKP
                     TTEEEDADESPAASAAGLERSAARSELGSIASDEPEPETAQPSPKTPAPTRQRAPRPE
                     PVPAAAPPAASITEPEPAPAAAQKEEAEQDSTTGAVAGISYWKCPACGVINGRTAQIC
                     RMCFGPRP"
BASE COUNT          844 a         1954 c         1892 g         1266 t
ORIGIN      
        1 ggaactgcgc gaacttgcag atgccgtgac gagcaaagga gtgacgcccg agatcgagtt
       61 cgccgggcgt ctcgatcatg acccgctgtc caagctcctg cccacgttcg atcttctcgt
      121 ggtcccatcg gtcgtgccgg aggctttcgg gatggtcgcc gccgaagcgg ccgcgtgcgg
      181 tgtcctgcct ctcgttccgg gtcactccgg catcggcgag gtcggagcca cgctcgaagc
      241 tgccctcgac cgccccggct tgcttacgtt cgatccactc gatcccgtgc gagacatcgc
      301 gcgaggtatc gaccgggtct tggcgttgga tccccgtgac cgccgcgaac tcggccggag
      361 ggcggtccag gtggcgcgcg agctgtggtc gtgggacgga gttgccgctc ggctattggc
      421 ccttgccacg gactaggctc gacgcatgtc tacgttcacc tacgaaggca ccgagaaccg
      481 catgcggtgg gatcggcgcc gcaccggctt catggaggtc tggtacgcaa cactcaatca
      541 ccggcccagt ggttccgggg cgtggttccg atacacgctg accgcacccg accattcggt
      601 gggcgccccg tactgcgagc tatgggggtt cttcttcgat ccggacggca aacggagctt
      661 cgcggccaag aagaggttcc cgatcgatca tctcggtgca ccgaatgggc gcgatgacgg
      721 ggcgctcgtt cggatcggtg attcgtggtt gtccgagacc cacctcgagg gcgagcttgc
      781 ctccgagcaa gggagcctga ggtggtcgct cgacttcgag cctgcggatc gatgcttcca
      841 acacctgccc gagcaacttc gcacccgggt cgaggatcgc gtttcgaccg tgtgctctcc
      901 caacctgtcg gtgccattca ccggaacggt tgaagtcggt ggcgaggtat tcgacttcga
      961 ggccgagcct ggctgtcaat cgcatcgctg ggggcggcgc cattcgcaga cgtggagctg
     1021 gggacattgc gccaccttcc aggaggaccc cagcgcgatc ttcgaaggag ttgcggcgcg
     1081 cgccaacatc gggccggttc cgggtcccac gacgacgttc ctctacctgc atcagggggg
     1141 tgaggacctg gtcttcaacg aattgcgctg ggcgctccgt gcccggagcc gctacgagat
     1201 gccaacgtgg gctttcaccg caaagaacga ccggtggaag atcgcggggg ccgcgcgcgc
     1261 tcggctcgca gatctcgtgc aggtcgagta cgcagacccg gacgggtcgc cgcgcttctg
     1321 tgcgaactca gagatcgcgg atctggcgat cgaggtctac gaacgttcgg gctcgacctg
     1381 gcgacaccac aacagcttga ccgctctagg aaccggtcac gtcgagttcg gtcgaccgca
     1441 ggcgttctcg gaactacccg tttccttcta ggtctctcac cgagcgacgg ttcttcttga
     1501 ggtcgcttcg tttccgtttc gctttcaacc gcctctcctt ggccgctttc gtcggtgcgg
     1561 ttccgacgcg ggccttgggg ggcttgagcg cggcggcaag cagaccggcc aagcggctcg
     1621 ctgcttcctc gcgatttcgc aactgggagc ggtgacggtc gcttgccacg cggatgttcc
     1681 cgttggcgtc gatcctgcgt ttcagccccg ctttgatccg gtcccgttgc cgggggccga
     1741 ccgcccgtga gtcttcgacg ttccacacca gttcggctcg ggtagacgac cggttcgcat
     1801 gctgacctcc cggccccccc gagggggaga acgtgaactt gatctcgtcg tcgggtatca
     1861 cgagcgatcg cgtgacccgt atccccttca tcggtcggcg tcgagttgtc gtgcgagttc
     1921 ctcgggcgtc gtgacgggcg catggcacac gccccggtga caaacgtatg cagtggcaag
     1981 cccgtcggct ttctttcgat cccggaacaa tgggagatcg acggtcccct cgtttcccac
     2041 gagtaggagt tgaccgggat cccagcgagc tctgacggtc tcaaccagtg cctccgttcc
     2101 gtggtcccct tccgctccga cgatgaccac ttcgcgcggg ttgcccgtgt agaaatcgac
     2161 ggcttccaac aggtgcgcga aggcaagtgg cgagttgatg gccgcctcgc gcagcaagcg
     2221 catcacttcc agagagcgat cttcgtaacg gttgtctccg gtcagaaggg tcagtttttg
     2281 aagctggaga gccatgacgg agttcgccga gggcacggca ttgtcgatca catccttgga
     2341 tcgggtgacg agctgctcgg cgtcgttccc cgcggtgaag aaaccgggcc cggcctcgtc
     2401 gtggaacagg gttatcgcag cgtcggcggt ggaacgagcc cgctcgagcc actcgacatc
     2461 ctgagttgcc tcgaagaggg cgagacaggc ctcgatcacg gccgcgtggt cttcgcagaa
     2521 accgagatgt tgcgctcgac cctcccgata cgagcgcatc aatcgaccgt cgcgtacgag
     2581 tccgtcgaag gtgaagcgca tcgcttcgct cgctacctcg atccactcct tccggctgaa
     2641 gatcgtcccg gcttcggcga acgccgcggc ggtcagggcg ttccaagccg tgatcacttt
     2701 cgtgtccgtc ccggggcgaa cgcgctgatc gcgtgcttgc atcagcgtct ctctccaacg
     2761 cccgagaacc tccggctcgg gctcggcccc cgtggccagc aggatgttct tgccctcgaa
     2821 gttgccctcc ggcgttacgc cccagtaggc ctcgcccgcg gaggattcgt cacccagcaa
     2881 ggcgcgtatc tcgtcgggct cccagacgta gaacttccct tcgacgcctt cggagtcggc
     2941 gtcgagcgac gaccagaacc caccctcggg gtcgcgcatc tcgcgcagca accatcctgc
     3001 ggtcagggcc acgatctcgg catagcgggg gttgtggtct tgcgcccaag ctcgcgcgta
     3061 cgtgcgaagc agttgcgcgt tgtcgtagag catcttttcg aagtgaggca cgatccacgc
     3121 ccggtcgacg gagtagcgat ggaagccccc accgatctga tcgaagatcc ccccggcagc
     3181 catccggtcc agggtcgata tgtacaaagt tctggcatcc ggccggtccc tgagagcgag
     3241 tcgtatcgcc aggtcgagag tcatcggttg agggaacttg ggtgctcctc cgaacccacc
     3301 gtgatccggg tcgaaattct gcctgagctt gtcgaacgcc tggtgaagat cggcgcgggt
     3361 cacatcgtcc gaagatggct cgatccggcc gatcgggttc agatgttcga cgagacgctt
     3421 cccctgcagc tcgacgtcgt cccggcgcga ctgccacgcg tcggtaatgg cgtgcagcac
     3481 gttggagaac gacggcatcc cgtaacgctc ctccggaggg aagtaggtgc cgccatagaa
     3541 aggcaaaccc tgcggcgtga ggaagaccgt catcggccac ccgccttgcc ccgtcatggt
     3601 ctgcacggcc tccatataga tggcgtcgat atccggacgc tcttcgcgat cgaccttcac
     3661 cgccacgaag tgctcgttca tgatcgctgc cgtggcgggg tcttcgaacg attcgtgctc
     3721 catcacatgg caccaatgac aggccgcata gccgaccgag aggaggatcg gcttgtcctc
     3781 gtctcgcgct cgggcgagcg cttcttcacc ccaggggtac cagtcgacgg ggttgtcttt
     3841 gtgctggatc aggtaggggc tggtttcggc agccagccgg ttcgccatgg caacatcatc
     3901 ccgcgccgtc gcgacggggg ctgacgtcgc taccctggga cgatggccgc tctcgcgtac
     3961 gttctgcttc ctttctcggg actcctctgc tacctactcg gccactcgcc ccgggttcgg
     4021 ttccacggtt tgcaggcgat agcgctgggg gcgttgtggc ccgccttgct ctacgttgcc
     4081 ggcgggatct cggagcaagc gacgcgactc gtggcgttga tcggcgcgat cgtttggatc
     4141 ggcatcacga tcgctaccgc ggcgggcaag gatctgcacc ttcccgttct cggtgctcgc
     4201 ttgcgcaggg tcgccgaggt ggagcggagc taaggccgag ggccgaaaca catccggcag
     4261 atctgcgcgg ttcttccgtt gatgaccccg catgccgggc atttccagta agagatcccc
     4321 gcgaccgcac cggtcgtgct gtcttgctcg gcctcttctt tttgtgcggc ggcgggtgcc
     4381 ggttcgggtt cggtgatcga cgccgcaggt ggggccgcgg cggggacggg ttcggggcgc
     4441 ggcgcgcgct gccttgtggg ggccggagtt ttcggagacg gttgtgccgt ctcaggttcc
     4501 ggttcgtccg aagcgatcga acccaactcc gagcgcgcgg ccgaccgttc gagcccggcc
     4561 gcgctggcgg ccggagactc gtctgcatct tcctcttcgg tggtcggctt ccattccccg
     4621 gtctgttcct cgaagaccaa cagttcgccg tccctccgat accaccagcg gccgtccttc
     4681 tggaagggtc gtccctcaac tgcctcggct tcgtctgctt tgcgaccgaa tagcttgctc
     4741 ggcggccggc gcgctttatc caattgcttc ttggcggagc gcgtcgccga ttcctcgcgc
     4801 tggcgccccg aacgcgcttc cttgatcgcg gcttccaatt cggttcgctc ggcttgtatg
     4861 gaagccaccg acgagcgctc ctgctcgcgt gtctgccgca accgttcgat cgaggctagt
     4921 tccttctgca gttcggcctt cgccgtctcg atctcgcccg acaggcgggt cgcctccgct
     4981 cgcgcggtct ccgtttccgc ggtcaggttc tgaagctcgg tctctgccgt cttaagtgag
     5041 gcctgctcca cctccaaccg ctggcgtgcc gcggtctcct ctgcgtcacg tcgggtccgg
     5101 tccgacgcca gtgcctcttt gtcgtccgcg agtttccgta tccgagcctc gatctgttcc
     5161 ttctcctgtt tcaatcgagt ctgcagggcg tcgagctctg tggaggccgc ggccgcatcc
     5221 tcgctcgtct tgtcgagctt ggattgagcg gtggtcagtt cggtgcgagc ggtctcggcg
     5281 gctttcttct cggccgcttg cctggtgcga gcgatcttta tctgcttctg cagctcggtt
     5341 acttccgcca gcgtttgctc gatcgccgat tcctcggttg cgaggtcacc gatcagcgtt
     5401 tgcagtgtcg cttgtgctgt tgctcgatct ttacgcgcga cctcgaggtc ggcttctgct
     5461 ttggcgcgtt cttggcgagt gtcgtcgaga ccggtctctc tgtcgcgctc cttgagcagt
     5521 tcgtcgagcg ccgcgcgctc cttgtcggcc tccttgcgta ggtccgcgcg agccgctcgc
     5581 ttcctctcga tctcctgacg agtcgtctcc agcgaagctt gctcctgatc gtgctcgtga
     5641 gccgcctcgg ccgcgctctt ctcgagttcg gctcgctcac gcctcgccgt gtcggtcgcg
     5701 gcccggtgct cgtcgcgttc cttccacatc gccttcaccg cggcctgttc ctctgagcgt
     5761 tcgcggcgag cgtcttcgag cgcggcctct gctttcgccc gctcttcctg tgccgaatcc
     5821 gccgagatct cgtgctcacg ttgtttcaga agctcgtcga gcgctgcctt ctgctcggta
     5881 ctttccttgc gaagttcgga cagcgtcttg cgctggcgtt ccagctcgag gcgggccgcg
     5941 tcgagcgcgg cctctt
//
LOCUS       DATMED010000328         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3722612, whole genome shotgun sequence.
ACCESSION   DATMED010000328 DATMED010000000
VERSION     DATMED010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722612"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1260)
                     /locus_tag="VNP73_08460"
     CDS             complement(166..1260)
                     /locus_tag="VNP73_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65992.1"
                     /translation="MAGAFLSLALALSTTAVGGAAPSKQSDVELVGAFVVGAPLTGAA
                     LSGDRLYLTTIDRLSIYDIGDPANPVQLASEPSPHPIHGERISTDDRLLLLNDPFLNF
                     TVDVWDVEDEGNPILVSTVRGVDDEHFSCLLDCRWAYGSAGSVIDLSRPSKPVLKDFD
                     WKSHLDLPDDVWIHRVDEYKRGFMVTAPRPGDAPVIIDARQPLNPKIIARVVFPPMNR
                     RGFLYSTWPNEGKSRFLFSSVEYSNSRNCSGDEEGALISFDAKGWPAKWQFQEADRFK
                     IRGAGEGSQDCASYYFSLHPRFEQTGLILVPYGREGVRIVEVGKQGRMSEVGYFDTAL
                     FDIWHSLWASDDIFYAADYTGAVYVLRFVR"
     gene            1378..1560
                     /locus_tag="VNP73_08465"
     CDS             1378..1560
                     /locus_tag="VNP73_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65993.1"
                     /translation="MRRLRMVVLSMILAVGGAFALASPAQAGPPQDCHTGDLPDPCPV
                     LYIVCNTKPGQVVCRD"
     gene            complement(1564..2028)
                     /locus_tag="VNP73_08470"
     CDS             complement(1564..2028)
                     /locus_tag="VNP73_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65994.1"
                     /translation="MSTRSLAVTAALLVVFSAAGASARPVATSIEREDEKLYVAPSAA
                     FLDQLGTVGVCEAGLGCVQFGTNATDDRVRIRLVDATSERVAGIVWQRRPGAPPTSRV
                     TALATLCSESGALHIPWPGQPVFVGILGGRCDNGLPSTPTTGTATASFWTSD"
     gene            complement(2091..>2605)
                     /locus_tag="VNP73_08475"
     CDS             complement(2091..>2605)
                     /locus_tag="VNP73_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65995.1"
                     /translation="KRSRPREWWVRLESGDLVIDRPLHAGNAYSYPRALGASDLDGDG
                     RFEWWIKVVDYTSHGAPWSGANVFFVQDEALVPLKVDGKPLDINFGGIARLGEGAACR
                     DNRLVLLRAEAQNRRNTRWSVSERSFELDGDEARLVERDEDLLRIGGYNDPDLDPYYE
                     IDCNGFGYPS"
BASE COUNT          512 a          838 c          790 g          465 t
ORIGIN      
        1 acaaccagcc agctatgtcc acccaggttc gatgattatc gacgagggca ttccagaccg
       61 acgaggcgac gatcaccaca agtaatgcga gaaccgtcca gggcctcgga ccaacgcctc
      121 cgggacgatt ctcgatggat cgagctagat cctcgctcgg gactatcaac ggacgaatct
      181 cagcacgtag acggcacctg tgtagtcggc cgcgtagaag atgtcgtcgc tcgcccataa
      241 cgagtgccag atgtcgaaga gcgcggtgtc gaagtaaccg acctccgaca tgcgaccttg
      301 cttgccgacc tcgactatgc gtacaccctc acgcccataa gggacgagga tcagccccgt
      361 ctgctcgaac ctgggatgca gcgagaagta gtagctcgca cagtcctgac tcccttcgcc
      421 ggcgccgcgg atcttgaagc gatcggcttc ttggaactgc cacttggccg gccagccctt
      481 cgcgtcgaac gagatcaacg caccttcctc gtctccgctg caattgcgag agttcgagta
      541 ctcgaccgac gagaacaaga agcgagactt cccctcgttg ggccacgtgc tgtagaggaa
      601 cccccttcgg ttcatcgggg ggaagacgac tcgggcgatg atcttcgggt tgagtggttg
      661 ccgcgcatcg atgatcaccg gagcatctcc cggccgcggg gcggtcacca tgaagccgcg
      721 cttgtactcg tccacccgat ggatccagac gtcgtccggc agatccagat ggcttttcca
      781 gtcgaagtcc ttcaacaccg gtttggaagg gcggcttaga tcgatcaccg atccggccga
      841 gccgtaggcc cacctgcagt ccaacagaca cgagaagtgc tcgtcgtcaa cgccccgcac
      901 ggtcgacacg agtatggggt tgccctcgtc ctccacgtcc cacacgtcca ccgtgaagtt
      961 caggaacggg tcattcagca acagcagacg atcgtcggtg gagattcgtt caccgtggat
     1021 ggggtgcggg ctcggctcag aagcgagctg aacgggatta gccgggtccc cgatgtcgta
     1081 gatcgagagc cggtcaatcg tcgtcagata gagccgatcg ccggacagcg cggcccccgt
     1141 taggggagca ccgaccacga acgcgccgac gagctcgacg tctgactgtt tggacggagc
     1201 ggctccaccc acggcagtgg tggacaacgc caacgccagg ctgaggaacg ccccagccac
     1261 cccccggagg ctcatgacca aagcattctc caggaccgag ccctcccctg tcccatttgc
     1321 acctttagcc ctgcatcgat tagaagggca gccgatcccg accaggaaag aggcgatatg
     1381 cggcggctga gaatggtggt tctgtcgatg atccttgcgg ttggaggggc gttcgctctc
     1441 gcaagcccgg ctcaggcggg tccgccgcag gattgccaca cgggagatct gcccgatccg
     1501 tgtcccgtgc tctacatcgt ctgcaatacg aagccgggcc aggtcgtctg ccgcgactag
     1561 gagctaatcc gaggtccaga agctggccgt agccgttccg gttgtcggcg tgctgggcaa
     1621 cccgttgtcg caccggcccc caaggatgcc gacgaacacc ggctgacccg gccacgggat
     1681 gtgtagcgca cccgactcac tgcacagcgt cgcgagtgcg gtaaccctcg aggtcggggg
     1741 agctcccggc cgtcgttgcc acacgatccc ggccacacgc tccgaggtgg cgtctaccaa
     1801 tcggatacga acccgatcgt cggtcgcgtt ggtgccgaac tggacgcatc caaggccagc
     1861 ttcgcagaca ccgaccgtgc ccagttggtc gaggaacgcg gccgacggag cgacgtacag
     1921 cttctcgtcc tcccgttcga tcgatgtggc gacgggacga gcactcgccc cggctgcgga
     1981 aaagacgacc agtagagcag ctgtgaccgc cagcgatctt gtcgacattc ggacctccac
     2041 tcgttgtgtt cttgctgggg agtacttctg ggaggcccct cgggtaacgg tcaggaggga
     2101 tagccgaagc cgttgcagtc gatctcgtag taggggtcga ggtccggatc gttgtagccg
     2161 ccgatccgta acaggtcctc atcacgctcg acgaggcggg cctcgtctcc atcgagttcg
     2221 aaagacctct ccgagacact ccaacgcgtg ttgcgtcggt tctgggcctc ggctcgcagc
     2281 aacaccaaac gattgtcgcg acaggccgcg ccctctccaa gtcgagcgat cccaccgaag
     2341 ttgatgtcca ggggcttgcc gtcgaccttc aacgggacga gggcctcgtc ctgaacgaag
     2401 aacacgttcg ctcccgacca cggtgcaccg tgactcgtgt agtcgacgac cttgatccac
     2461 cactcgaagc ggccatcgcc gtcgaggtcg gaggctccca gtgcacgtgg gtagctgtag
     2521 gcattgccgg cgtggagagg tcgatctatg accagatcgc cagattcgag acggacccac
     2581 cactccctcg gcctcgatct cttgt
//
LOCUS       DATMED010000329         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1805315, whole genome shotgun sequence.
ACCESSION   DATMED010000329 DATMED010000000
VERSION     DATMED010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805315"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VNP73_08480"
     CDS             <1..1141
                     /locus_tag="VNP73_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HWL65996.1"
                     /translation="DLPFGESVAVGLSDLEAAMAKSGNDNLVIFGHSQSSVIAYLEKR
                     RLAEQFPIGTPAPDIDFVSIGTLNLPNGGVMTRLWGLYLPFVDFYFNGPAPTDTQFDT
                     DIITQRWDGFADFPIYPIVLPSVVNAVAGFFYTHEDYEAVTLAPDPSTYFVGKQGDTE
                     YYFFETKDLPLYGPLRGFGVPEQLIDVIEPLSIFVVELGYRRDIKPWVPSPARLIPIH
                     NPFKVVGDFLEAVDETIDNTWKVLGSNTTEQNADTSTGRRAMTAEAAPVLLSQDEPSA
                     PEPTVLESEAESDVPQDDNESASSEDDENVASEGDQAETAIGEDDPSTTIPDAEADED
                     EEQDETATDSPESPANNDDSSPSVDGASTDDSSAGDNSNADGDPS"
     gene            1562..2041
                     /locus_tag="VNP73_08485"
     CDS             1562..2041
                     /locus_tag="VNP73_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65997.1"
                     /translation="MKKLIARGAKVVAVGAVAASFAMLGAGAATADPLIGKTYSDASG
                     MVADQWNATPVVATVSGDQLSTDQCIVSSWRRSTSIDALTGRNSQNVLLHLNCNAKVA
                     EAGVPGNSAATAVGKQAKQDINRANSINKDPDACYESDANLTYCKRICDRTGLCEIE"
BASE COUNT          474 a          707 c          700 g          363 t
ORIGIN      
        1 cgacctgccg ttcggcgaat ccgtggcggt tggtctcagt gacctcgagg ccgcgatggc
       61 caagagcggc aatgacaatc tggtcatctt cggccactcg cagagctcgg tgatcgccta
      121 tctcgaaaag cgaaggctcg cagagcagtt cccgatcggc acaccagccc ccgacatcga
      181 cttcgtctcg ataggaacgc tcaatctgcc caacggcgga gtgatgacgc ggttgtgggg
      241 gctctacctg ccgttcgtgg acttctattt caacggccct gcgccgaccg acacccagtt
      301 cgacacggac atcatcaccc aacgatggga cggcttcgcc gacttcccga tctaccccat
      361 cgtcctgccg tcggtcgtca acgcggtcgc tggcttcttc tatacgcacg aggactacga
      421 ggccgtcaca ctggcgccgg atccgtcgac gtacttcgtc ggcaagcagg gcgacaccga
      481 gtactacttc ttcgagacca aggacctgcc gctctacggc ccgctgcggg gcttcggcgt
      541 accggaacaa ttgatcgacg tcatcgagcc gctgagcatc ttcgtggtcg aactcggcta
      601 ccgccgcgac atcaagccct gggttccctc gcccgccagg ctgattccga tccacaatcc
      661 gttcaaggtc gtcggcgact ttctcgaggc ggtcgacgag accatcgaca acacatggaa
      721 agtgctcggc tcgaacacga ccgagcagaa cgccgacacc tcgactggac gccgcgccat
      781 gacggccgaa gccgcaccgg tgctgctgtc acaggacgaa ccgtcagcac ccgaaccgac
      841 ggtattggaa tcggaagccg aatccgatgt gccgcaggac gacaacgaga gcgcctcctc
      901 cgaggacgac gagaacgtcg cctccgaggg ggaccaggcc gagacggcca tcggcgagga
      961 cgatccgtcg accacgatcc cggacgctga ggcggatgag gacgaggagc aggacgaaac
     1021 cgcgacggat tcgccggaaa gcccggcaaa caacgatgac tcgtcgccat ccgtcgacgg
     1081 cgcgtcgacc gatgactcat cggcgggcga caactcgaat gcagacggcg atcctagtta
     1141 ggccgcgaca acctcgtcac gcgcgaacca cgggcggtac tggtcgaagg tgggccgaca
     1201 gattgagatc atcaatgtga ttgtgccgct ggtgaattac ggccaggcta cccaggaccg
     1261 cttcaacgca ctgaacgagg agaaggccga caccgggtgg gcgacgagcg ggccagtgcc
     1321 tggacgcgga gcgggaagcg aacagcagcc cgctaggggt gctggccgaa tatcaccgcg
     1381 gtgccgaccg ttcctgcacg ctgagtggtg gcccggacat cgaagcattt ctagacggtt
     1441 tgctcgccag acaatgatgt aacgtaacgc gacgttgccc cggggcgatc tttcttttgg
     1501 ctgactcgta gtccctcaga aggccagttc agcagtgagc agacacggag gtcgcgaacg
     1561 cgtgaagaaa ctcatcgccc ggggagcaaa ggtggtcgcc gtcggcgcgg ttgccgcgtc
     1621 atttgcgatg ctcggggccg gcgcggccac ggcggatccg ctgatcggca agacctattc
     1681 ggacgcctcc ggaatggtcg cggaccagtg gaacgcgacc ccggtggtcg ccacggtcag
     1741 cggcgatcag ctgtctacgg accaatgcat cgtctcgagt tggagacggt cgacctctat
     1801 cgatgcccta acaggcagaa acagccagaa cgttctgctg cacctcaact gcaacgccaa
     1861 ggtcgccgag gcgggtgtgc ccggaaactc cgcggcgaca gctgtgggca agcaggcgaa
     1921 gcaagacatc aaccgggcca actcgatcaa caaggacccg gatgcctgct acgagagcga
     1981 cgccaacctc acctactgca agcgcatctg cgaccgcacg gggctgtgcg agatcgaata
     2041 ggtagcgctc tctgagcttt gcgatgagtt cccggccgct taggggtcaa atataggaca
     2101 cccgaggagg ccggccatgg gcaagatctg cacgcatatg acgatgtcgt tggacggttt
     2161 catcgccgac tccgacgatc ggcccggcga actcttcgac tggtaccagg ccggagaggt
     2221 gtcggtgtcg aaccctaacg aaga
//
LOCUS       DATMED010000330         4061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3351221, whole genome shotgun sequence.
ACCESSION   DATMED010000330 DATMED010000000
VERSION     DATMED010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3351221"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VNP73_08490"
     CDS             complement(<1..1724)
                     /locus_tag="VNP73_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65998.1"
                     /translation="MRALLRATIVALTVAVLAGPFAAARGFAAPPCPGVSTEGAWTTI
                     SAPDFPAGATAIASYAVDPNRPTVIFASNGTHVVRSSDGGCKWTAVFALELIPTLDRQ
                     VSSANTSIKDIVIPEGKSSVYLTLEEKVGPVVRPHIAVSDDSGVNWQLNNTGLTPASG
                     GILSLVAAPSNARVMYLLLRSNPVGAENALYASTDGGATWEQRSEAADGSSNLAVDPL
                     APDDLWLWGGRLMESKDGGRTRAIHTETHNVSMADVSHTPGTPARVMGYEPETQTMVI
                     VTDGSSTWTRIFGPQTFSHSMTNTGGGPFDVVVAGNGGVFQMVAPEFWVAIDPPNQPD
                     IYDLQSTRVGPPLVFGYAPETNELVRYEAQKVDVDPFTTVSDGIVASTTELSPKRKTV
                     KLKPGESRTVRYNLGLPPHPTPLDVFFLVDTSNSMESTINGLREGMQQVIDELEDSRM
                     DVHFGVGEFKDYPIPGFGNPEAGDFPYRLNRSIGPAGPELAEALEKLEASGGGADPRE
                     SQLTGLYQAATGDGEPGFVPAGQDAGFRPAALKVIVHITDAAFHTDPAHPSPPWATVV
                     DALTTKGIL"
     gene            complement(1842..3044)
                     /locus_tag="VNP73_08495"
     CDS             complement(1842..3044)
                     /locus_tag="VNP73_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL65999.1"
                     /translation="MRKGLVAAAAAAIAVATFAMPVDAKRNPAPLKGEGKNMKIVANI
                     PYAGGTDMEFATIKGRDYAFAGSFAQVKDGGGLHIIDITTPAKPKEVAWLKCSVNQND
                     IQITFDQKHVLMAADSAGGPESCLAVGKEGFLIVNIENPAKPKVVSFAETRSSHNTTA
                     HPTKPYVYNSSSLNGTNGIIQIWSIKNPAKPELVTNYQAIPLAPHDISFNKDGSKAVT
                     ASINHFDILDTSDPEAPMITFTGQCPGCSITHDAKFTPDDTGIVIGDEGGGGGGYPCP
                     GGALYFYDYSSGTVPVLTGVYEPNEILFAREGQSDLGGCTSHVFDFDDKGEHISISWY
                     TAGTRYLDITNPVGPTFGENGVGVVETGWFMPDGGDSWSSKIYTDPKYIFSNDIARGF
                     DVFQVTAK"
     gene            complement(3074..3778)
                     /locus_tag="VNP73_08500"
     CDS             complement(3074..3778)
                     /locus_tag="VNP73_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66000.1"
                     /translation="MKAKKLVALLIGATLVGALVAGPASIASGKAKAAVVGTDAVGDW
                     GTNGPNGDASLNAIGDAQGMDLVSASIEPADAKTLNFVLTMNSLPPSGGIPEVARYTW
                     DFNVDGEFRELDGKFTNYSRGTCDPTAGSCPPPRDPGMQPFFLRGNCGITETPAISLT
                     TCEELAAIQGVFDSAAKTITIPVPLAALGAKKGSKIEPGTNIFGGSVSASPSAFITNN
                     SMPMDTLVVTKTYVVR"
BASE COUNT          654 a         1210 c         1375 g          822 t
ORIGIN      
        1 aggatgcctt tcgtggtcaa cgcatcgacc acggtggccc acggaggtga agggtgggcg
       61 gggtcggtat ggaaggcggc gtcggtgatg tgaacgatca ccttcagagc cgcggggcgg
      121 aaacccgcgt cctgaccggc cggtacgaag ccgggttccc cgtcgccggt tgcggcctgg
      181 tagaggccgg ttagctggga ctcccggggg tcggcgcccc caccactggc ttcgagcttc
      241 tccagcgctt cggcaagttc gggccccgcc ggaccgatcg agcggttgag acggtagggg
      301 aagtctcccg cctcggggtt cccgaagccc gggatcggat agtccttgaa ctcgccgacc
      361 ccgaagtgca cgtccatccg ggagtcctcc aactcgtcga tcacctgttg catgccctca
      421 cgcaggccgt tgatcgtgct ttccatgctg ttggacgtgt cgacgaggaa gaacacgtcg
      481 agcggcgtgg gatggggcgg gagcccgagg ttgtagcgaa cggtacggct ctcgcccggc
      541 ttcagcttca cggtcttgcg tttgggactc agttccgtcg tgctcgcgac tatgccgtcg
      601 gacacggttg tgaaggggtc gacgtcgacc ttctgggcct cgtatcgaac gagttcgttc
      661 gtctccggcg cgtatccgaa gacgagtggt gggcccaccc gcgtcgattg gaggtcgtag
      721 atgtccggtt ggttcggggg atcgatggcg acccagaact ccggcgcgac catctggaag
      781 accccgccgt tgccggcgac cacgacatcg aagggaccgc cgccggtatt ggtcatcgaa
      841 tgggagaacg tttggggacc gaagatcctt gtccatgtgc tcgatccatc tgtgacgatg
      901 accatggtct gcgtctccgg ctcgtatccc atcacgcggg ccggcgttcc gggcgtgtgg
      961 gagacgtcgg ccatgctcac gttgtgggtc tcggtgtgga tggctcgagt gcgaccgcca
     1021 tccttcgatt ccatcagccg gccgccccac aaccagaggt cgtcgggcgc gaggggatcg
     1081 acggcgaggt tggacgaacc gtcggcggct tcagagcgtt gctcccaggt tgcgccgccg
     1141 tcggtgctcg cataaagagc gttctcggcc cccaccgggt tcgagcgcag caagaggtac
     1201 atgacacggg cgttgctggg tgctgctacg agactgagga tgccaccgct cgccggagtc
     1261 agacccgtgt tgttcagttg ccagttcact cccgagtcgt ccgataccgc gatgtgtggc
     1321 cgcacgacgg ggccaacctt ctcttcgagg gtgaggtaaa cgctgctctt accttcgggg
     1381 atgacgatgt ctttgatcga cgtgttggcc gacgagacct gtcgatccag cgttgggatc
     1441 agctccagcg cgaagaccgc ggtccacttg cagcccccgt cgctcgaccg cacgacgtgt
     1501 gttccgttgg aagcgaagat cacggtgggt ctatttgggt cgacggcgta ggacgctatc
     1561 gcggtagctc cggcagggaa gtcgggggct gagatcgtcg tccatgcccc ctcggtggag
     1621 acaccggggc aggggggagc cgcgaacccg cgggcggccg cgaagggacc ggccaggacc
     1681 gccaccgtga gggcgacgat cgtggctcgt aggagagctc tcatctagaa agaagtttcg
     1741 ccggtctgag ggggtgtcct tcgaggcccc gccagagggc cacaggaaag accccgggtt
     1801 gccccggggt ctcctcctag cccgccttac ctggtctgcc gttacttggc agtgacttgg
     1861 aagacgtcga accctcgagc gatgtcgttc gagaagatgt acttggggtc cgtgtagatc
     1921 ttggagctcc acgagtcccc gccgtccggc atgaaccagc cggtctcgac cacaccgacg
     1981 ccgttctcgc cgaacgtcgg gccgacgggg ttcgtgatgt cgaggtaacg agtgcctgcc
     2041 gtgtaccacg agatcgagat gtgctcgccc ttgtcgtcga agtcgaagac gtggctcgtg
     2101 caccctccga ggtcgctctg accttccctc gcaaagagga tctcgttcgg ctcgtagacg
     2161 ccggtcagaa ccgggaccgt gccgctcgag tagtcgtaga agtaaagagc gccacccggg
     2221 cacgggtagc caccgccacc gccaccctcg tcaccaatga cgatgccggt gtcgtcgggc
     2281 gtgaacttgg cgtcgtgcgt gatcgagcaa cccgggcact gaccggtgaa cgtgatcatc
     2341 ggagcctcgg ggtcgctcgt gtcgaggatg tcgaagtggt tgatcgatgc cgttacggcc
     2401 ttcgatccgt ccttgttgaa cgagatgtcg tgcggcgcaa gcgggatggc ctggtagttc
     2461 gtgacgagct cgggcttggc cgggttcttg atcgaccaga tctggatgat cccgttcgtg
     2521 ccgttcaacg agctggagtt gtagacgtac ggcttcgtcg ggtgcgcagt cgtgttgtgc
     2581 gagctgcggg tctcggcgaa gctgacgacc ttcggcttgg ccgggttctc gatgttgacg
     2641 atgaggaacc cttccttacc gacggccaga caagactcgg ggccaccggc ggagtctgcc
     2701 gccatcagaa cgtgcttctg gtcgaaggtg atctggatgt cgttctggtt gaccgaacac
     2761 ttcagccacg cgacttcctt cggcttggcc ggggtggtga tgtcgatgat gtggagaccg
     2821 ccaccgtcct tgacctgtgc gaaggagcct gcgaaggcgt agtcgcggcc cttgatcgtg
     2881 gcgaactcca tgtcggtgcc gcccgcgtac gggatgttag caacgatctt catgttcttt
     2941 ccctcaccct tgaggggcgc cgggttccgc ttcgcgtcga ccggcatggc gaaagttgcc
     3001 acggcgatcg ccgcagcggc tgcggctacg agacccttac gcatagtgtt tcctcccttt
     3061 tctgtcttgg ttgttagcga acgacgtagg tcttggtgac gaccagcgtg tccatgggca
     3121 tggagttatt ggtgataaac gcagacggag aagcgctgac cgaaccgccg aagatgttgg
     3181 tgccgggttc gatcttcgat cccttcttgg ctccgagcgc tgcgagggga accgggatcg
     3241 tgatggtctt tgcagcagaa tcgaagacac cttggatcgc tgcgagctcc tcacacgtcg
     3301 tgagggagat cgccggcgtc tcggtgatgc cgcagttgcc acgcaggaag aacggctgca
     3361 tgccggggtc gcgcggcggg ggacagctgc cggcggtcgg gtcacaggtt cctcgcgaat
     3421 agttggtgaa cttgccgtcg agctcgcgga attcaccgtc gacgttgaag tcccaggtgt
     3481 aacgagcgac ttcggggatc ccgccgctgg gcggcaggct gttcatcgtg aggacgaagt
     3541 tcagggtctt ggcgtcggcc ggctcgatcg aggccgagac gaggtccatc ccctgcgcat
     3601 cgccgatggc attgagagag gcgtccccgt tcgggccgtt cgtgccccag tcgccgaccg
     3661 cgtcggtacc gacgacggcg gctttggcct ttccggacgc gatggaggca gggccggcta
     3721 caagggcccc aaccaaggtt gcgcctatta gtagagcgac tagcttcttt gccttcatct
     3781 ccgtactcct ttgcacgacc tgctcattga tccgtgcagt cagaatttcg actcggagac
     3841 cttctctcct gcatgggcgg tgggtggcgc cccacatgtc cgtttcgtcc cctgtggcag
     3901 ggacgtgcca gcccgcggag aagtctttcg acgaccggtt gggaccaatc caggggggtt
     3961 cgatgaatcg cgttgggcgc tgtatcgctg cgatccttgc gctggcattg tccgcgcctg
     4021 cactcctcgc agctccctcg agcgcgggtg gcggacctcc c
//
LOCUS       DATMED010000331        10756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3727340, whole genome shotgun sequence.
ACCESSION   DATMED010000331 DATMED010000000
VERSION     DATMED010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3727340"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VNP73_08505"
     CDS             <1..494
                     /locus_tag="VNP73_08505"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66001.1"
                     /translation="LAEDVIDEGRACVLVLNKWDLITDDYERKVVERSLETKFRFLEW
                     APRVRTSALTGRGIGRILGALEVAIASHRLRLPTARVNTLIRDAQERRPHPRTGGRGR
                     RILYAVQASIAPPLFLLYSSGSLQTEYLRYLENRVREVEPFTGSPIKMETRLKARGER
                     ARR"
     gene            684..758
                     /locus_tag="VNP73_08510"
     tRNA            684..758
                     /locus_tag="VNP73_08510"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:719..721,aa:Pro,seq:ggg)
     gene            781..1404
                     /locus_tag="VNP73_08515"
     CDS             781..1404
                     /locus_tag="VNP73_08515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWL66002.1"
                     /translation="MDAEEPDGGPSAERRVLTIPNLLSFLRLASVPVFLWMWSSAASG
                     RRDGAVILYAIGAWSDFFDGYIARRTNSVTELGKLLDPLADRVFIIALAVALVIADVL
                     PAWLTGVIVARDVLVLAAFPVLEKRGLPRIAVSRVGKTATAALLSGLTLLAFSETSVA
                     GTDQGLFAGVILVGLTLMGVGAVLYWVAAVLYARDATRLWGTTKGAG"
     gene            1394..3949
                     /locus_tag="VNP73_08520"
     CDS             1394..3949
                     /locus_tag="VNP73_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWL66003.1"
                     /translation="MPGETGVDMQAVIMAGGEGSRLRPLTSNMPKPMLPVANRPLMEH
                     IITLLRKHGISDVVATVQFLSSVIRNYFGDGSDLGVSLSYATEDVPLGTAGSVFGARD
                     LLEGTFLVISGDALTDLDVKEVVDHHHRTGAAATLVLKHVPDPLEFGIVMTDEDGRVE
                     RFLEKPTWGQVFSDTINTGIYVLEPDVFDLIPPDEPYDFSSGLFPMMLDKGLPVFGFV
                     ADAYWTDVGNTDAYMQAHLDVLGGAVDVTIPGFELQPGVWVGEDVDIHPTARIEGPTL
                     VGDNCRIGAGAILSGYSVLGPNTILADDAQVSRSVVMEDTHVGAYARIHGAILGRGAS
                     LEKGASVEEGAVVGDEVQVGAGAIVKPRVKIYPSRSVEAGAIVTESVVRERRAQRSLF
                     GSHGISGLINVAVTPQMAVRLGMAYGSTLKRGSVVVAGRDSSRAARTMKRAIIAGLNS
                     AGVTCQDLELVPMPLTRFTVRNRQAVGGISVRTSPGDPESVEIRLFDSGGTDLSTGAQ
                     RKIERIYFREDYRRASAAKLGELEFPPRALEQYTSGLTRGLDIDAIRSLGAKVVVEYA
                     YGAASLIGPSFLGRLGCDVLAVNAFTDDHRPVLSDQEFVGLLEGLTEHVRKSGSDMGV
                     LLEPGGEIAHLVDGRGRVIPQERALLAFLQHAARNGATRLATPVSTSRVCEDVAAEAG
                     ATIEWSPISMPLLMERVGDGRVGFAGTPEGVFIWPEFMPAPDALMTFGKALELIAAAH
                     KPLADIVDELPPTHVARRSVRTPWQLKGTVMRELAAGHGVTDPSSRLVLIDGVKVLTG
                     DRWVLVAPYPDEPSCGVWAEAADAAASEELADEYAARVEEIVAAGDPGQHLDL"
     gene            4053..4439
                     /gene="gcvH"
                     /locus_tag="VNP73_08525"
     CDS             4053..4439
                     /gene="gcvH"
                     /locus_tag="VNP73_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWL66004.1"
                     /translation="MSEVPADLRYTKEHEWARRDDDRVVVGITDYAQDQLGDVVFVGL
                     PEVGADVEAGKPLGEVESTKSVSDVYSPISGRIAATNPEVEQNPELVNEEPYGRGWLV
                     AIEGGDIDGLMSADEYKALIEGAASA"
     gene            4504..4980
                     /locus_tag="VNP73_08530"
     CDS             4504..4980
                     /locus_tag="VNP73_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWL66005.1"
                     /translation="MYCTNCGHRNPEGSNFCSSCGAPLKDLKEGGDTDTTITFMPGEL
                     ESDLDEDIHISPDELEGGRGVLIVKRGPNAGSKFFIDTNVTTVGRHPESDIFLDDITV
                     SRRHAEIRRSNGSFGLHDVGSLNGTYVNRERVEETGLHSGDEIQIGKFKLVFLSGG"
     gene            4985..5728
                     /locus_tag="VNP73_08535"
     CDS             4985..5728
                     /locus_tag="VNP73_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWL66006.1"
                     /translation="MPLSRSRDYLSIGEVLETLQEEFPDVTISKIRFLESEGLIEPKR
                     TPSGYRMFFDKDLKRLHWILTMQRDHFLPLKVIKERIESGAGNGEALLAPAAPPAPAK
                     GAKGAKGAKAAPVAAAAPPPPDLTTVQLSRPELLKSAGLTERELASLEEYGIVGPGEG
                     GNYDGAALAMARAAKRFFDYGAEARHLKMYRQFAEREVAFFEQIVLPVSRRRDPAAQK
                     DAARSVRELAGLARQMRDAALHTSLQEHL"
     gene            5740..6906
                     /locus_tag="VNP73_08540"
     CDS             5740..6906
                     /locus_tag="VNP73_08540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWL66007.1"
                     /translation="MSTSRLLIGGVLAALVALPAPATAGFVAPQQSDPVVSCTACIVV
                     DDTGRVLWARAAHRRLPNASTTKMVTALEVVARSRQSEVALVSAEAASVGGGGLDLQA
                     GDEMTVRNLLFAMLLSSSNESAAALAEHVAPSQEAFVRAMNARARFLGTTDTHFMNPH
                     GLDATGHYSSAADLALIGAAVLDRPLLADIVATPSTSIMTPRGAVVEENRNVLLEGYP
                     GAIGIKTGRTIGAGNVLVAAATRGDRTVIAVAMHSTDAANDAAQLLDFGFARIERLDT
                     RVPLTLLLANTDVGSIVFDPSGATSVVAASSIRLDLPPGTDSFSYTLRPASVTAPLEA
                     GERVGTVSVSVDGRELGEVDAVAVDDVDRSEGDGLANALGTLMAAVATLVSAVT"
     gene            6903..7499
                     /gene="hpt"
                     /locus_tag="VNP73_08545"
     CDS             6903..7499
                     /gene="hpt"
                     /locus_tag="VNP73_08545"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWL66008.1"
                     /translation="MSQGAGTAHLLIAAGDIERRVTELAHEITADLAGAEPPLLIGVL
                     KGCTLFLADLVRAIDIDVHVDFIAISSYAGGTGSSGVVRILKDVEADIRGRDVLIVED
                     IVDTGLTLTYLRRNLEERGARSLRTVTLLDKAARRIVPVAVEYTGFEIEDVFVVGYGL
                     DFQGRYRNVTDVLAVTDIARLANDPLLLVEPLWPAVKR"
     gene            7521..7997
                     /locus_tag="VNP73_08550"
     CDS             7521..7997
                     /locus_tag="VNP73_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWL66009.1"
                     /translation="MIELSLVGVRVELPSNQPIVLLKETGGDRYLPIWIGAVEATAIA
                     FALQGIETPRPMTHDLMRDILAESQIQVDRIVISDLVDQTFYATISLSMNGRAMEVSS
                     RPSDAIALAVRLATPIFAAEEVIEQAGIELRDDEETEVQKFREFLDQVSPEDFAAG"
     gene            8252..8770
                     /locus_tag="VNP73_08555"
     CDS             8252..8770
                     /locus_tag="VNP73_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWL66010.1"
                     /translation="MDQALEVGYRGPQACKIVDITYRQLDYWTRTGLVSPSIQPAKGS
                     GTQRLYSFNDLLQLKVIKSLVDAGASLQKVRQAIDYVREELDADWAKVTIVADGSGVY
                     ACTSDAEVVDLLRSGQGVLGAIVAVGKVRDQLAGTLRELRPLERSVEYGDAKDPDGSA
                     PTTTPGSRAGEG"
     gene            9029..9235
                     /locus_tag="VNP73_08560"
     CDS             9029..9235
                     /locus_tag="VNP73_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66011.1"
                     /translation="MTKKNRKVRRLMQLYPDVKCKVLYQRDYLNLLVKYGLEEPSQGP
                     LIEADANSGPAEVHKVLLGPRLAG"
     gene            9242..10318
                     /gene="gcvT"
                     /locus_tag="VNP73_08565"
     CDS             9242..10318
                     /gene="gcvT"
                     /locus_tag="VNP73_08565"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWL66012.1"
                     /translation="MEGTARATVLLEEHRKLGAKLTEFGGWEMPLQYTGIVAEHRAVR
                     ETCGIFDVSHLGKLRVTGRDGGSALQEAVTADVESLEVGRASYALVLTEEAGCVDDVF
                     VYRIGEQEWLCVPNASNVAAVADMISAGGADAQDEWDRWTILAIQGPDSFSLFEEVFG
                     RSDALELALHSFCPLDVFGSVGIVARTGYTGERGFELYAPVDTAVKAFSSLLEAGGTP
                     VGLGARDTLRLEMGYALYGHELTLDTNPLEAGLGWAVDWDAPFRGRDALASIKETGPR
                     RRLMGIVCRDRGVPRQGYRVLSGEKQIGEVVSGNHSPTLGVGIALAYFPVEELPQPGE
                     EVAVEARGRSIVGDIVKPPFIKKR"
     gene            10350..>10756
                     /locus_tag="VNP73_08570"
     CDS             10350..>10756
                     /locus_tag="VNP73_08570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651323.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HWL66013.1"
                     /translation="MDFLPHTDADVADMLAVIGAASIEELFEPVPAGIRLDRSLDVPT
                     ALSEPELLRHLRELASASSGATDLVCFAGGGAYDHHVPSAVKALASRAEFATSYTPYQ
                     PELSQGVLQAVFEFQTLVCEIFGLEVANASLYDG"
BASE COUNT         1879 a         3426 c         3596 g         1855 t
ORIGIN      
        1 ggctcgccga ggacgtgatc gacgagggca gggcatgcgt cctcgtcctc aataagtggg
       61 acctgatcac cgacgactac gagcgcaagg tcgtggagcg ttcgctcgag acgaagttcc
      121 gcttcctcga gtgggctccg cgggttcgca cgtcggccct gaccggacgg ggtatcggtc
      181 gcatcctcgg ggcactcgaa gttgcgattg cctcccaccg tctccgtctg ccgacggccc
      241 gggtgaacac gctcatccgc gatgcccagg aacgacggcc gcacccgcgc accgggggcc
      301 gcggtcggag gatcctctat gcggtgcaag cctcgatcgc cccgccgctg ttcctgctct
      361 actcgtcggg ctcgctgcag accgaatacc tgcgctacct cgagaaccgc gtacgagagg
      421 ttgagccttt caccggatca ccgatcaaga tggagacgag gctcaaggcg cgaggcgagc
      481 gagctagacg ttaacttgac ccttagctcg atgtctgtca tgatgcgtct tgcttcgatc
      541 ggtcacgacg cagttgtctt caagacgcta aatcgataga tcgacgagtc cccacatctc
      601 cttgttgatt catctattca tcgacttatc tatttgtcga cttcagtggt gcgcgccccg
      661 tgaatatgat gcggcggaca tctcgggacg tggcgcagct tggttagcgc actggtctgg
      721 gggaccaggg gtcgccggtt caaatccggc cgtcccgact gaacaattac gctgcgtcca
      781 atggacgcgg aagagcccga cggcggcccc tcggcagagc ggcgtgtcct caccattcca
      841 aacctcttgt cgttcttgag gcttgcaagt gttcccgtgt tcctgtggat gtggtcctcg
      901 gctgcctcgg gacggcgcga cggggccgtc atcctgtacg cgatcggggc gtggtcagac
      961 ttcttcgacg ggtacatcgc acgccggacg aactcggtca ccgagctcgg gaagctcctc
     1021 gacccacttg ccgatcgagt gttcatcatc gccctggcgg tcgcattggt tatcgccgat
     1081 gtgctccccg cgtggctgac gggggtcatc gttgcccgtg acgttctggt tctggcagcc
     1141 tttcccgtgc tcgagaagcg ggggcttccc cggatcgcgg tcagccgggt cgggaagacg
     1201 gccaccgccg cgttgctatc cggcctaacc ttgttggcgt tctcggagac ctcggtcgcc
     1261 gggaccgatc agggtctatt cgccggggtg atcctggttg ggctaaccct catgggtgtg
     1321 ggggcagtcc tgtactgggt cgcagcggtg ctctacgcga gagacgcaac gcggctgtgg
     1381 gggacgacga aaggtgccgg gtgagacagg ggtggacatg caagcggtga tcatggccgg
     1441 gggcgaggga tcgcgcctga ggccgctcac gagcaacatg cccaagccga tgcttccggt
     1501 cgccaaccgg cccttgatgg agcacatcat cacgctgctc cggaagcacg gcataagcga
     1561 tgtggtcgcc accgtgcagt tcctctcgtc ggtgatccgc aactacttcg gtgacggatc
     1621 cgatctcgga gtttcgctct cgtacgcgac cgaagacgtc cccctcggca cggccggttc
     1681 ggtgttcggc gcgcgcgacc tcctggaggg aacgttcctg gtgatctccg gcgacgcgct
     1741 gacggacctc gatgtcaagg aagtcgtcga tcaccaccac cggaccggcg cggccgccac
     1801 ccttgtgctc aagcacgttc ccgacccact cgagttcggc atcgtcatga ccgacgagga
     1861 cggacgtgtc gaacgtttcc tcgagaagcc gacctggggt caggtgttct cggacacgat
     1921 caacaccggg atctacgtgc tcgagcccga cgtcttcgat ctcatccccc cggacgagcc
     1981 gtacgacttc tcttccggat tgttcccgat gatgctcgac aagggacttc cggtgttcgg
     2041 cttcgttgcc gacgcctact ggaccgacgt cggcaacacc gacgcgtaca tgcaggctca
     2101 tctcgacgta ctcggcggcg cggtggacgt gacgatcccg ggattcgagc tccagcccgg
     2161 tgtgtgggtc ggcgaagacg tggacatcca tcccacagct cggatcgagg gaccaacgct
     2221 cgtcggagac aactgccgga tcggggccgg cgcgatcctg tccggctatt cggtgctcgg
     2281 gcccaacacg atcctcgccg acgacgcgca ggtgagccgg tccgtcgtga tggaagacac
     2341 ccatgtgggt gcgtacgcgc gcatccacgg cgcgatcctg ggtcgaggcg cgtcgctcga
     2401 gaagggtgcc agcgtcgaag agggagcggt cgtcggcgac gaggtccaag tcggggccgg
     2461 cgcgatcgtg aagcctcgag tcaagatcta cccttcgcgg tcggtcgagg cgggcgcgat
     2521 cgtgaccgaa agcgtcgtgc gggaacgccg cgcccaacgc agcttgttcg gctcgcacgg
     2581 gatctcggga ctgatcaacg ttgccgtcac cccgcagatg gcggttcgcc tcggcatggc
     2641 ctacggatcg acgctgaagc gggggagcgt ggtggtcgcg ggacgggatt cgagccgggc
     2701 cgctcgcacg atgaagcggg cgatcatcgc cggcctcaac tcggcgggcg tcacgtgcca
     2761 ggacctcgaa ctcgtaccga tgcccctcac gcggttcaca gttaggaacc ggcaagcggt
     2821 gggaggtatc agcgtccgaa cctcgcccgg ggacccggaa tcggtcgaga tccgtctgtt
     2881 cgactctgga gggaccgacc tgtctaccgg tgcgcagcgc aagatcgagc gcatctactt
     2941 ccgcgaggat taccggaggg ccagtgccgc gaagctcggc gagctcgaat tccctccgcg
     3001 ggctctcgag caatacacgt cgggcttgac ccgcggcctc gacatcgacg cgatccggtc
     3061 actcggcgcg aaggtcgtcg tggaatacgc gtacggcgcc gcatccttga tcggtccgtc
     3121 cttcctggga cgccttggct gcgacgtgct ggccgtcaac gccttcaccg acgatcaccg
     3181 gccggtcctg tccgatcagg agttcgtggg ccttctggaa gggctcacgg agcatgtgag
     3241 gaagtccggc tcggacatgg gcgtgctgct cgagccggga ggggagatag ctcacctcgt
     3301 cgacggcagg ggccgcgtga tccctcagga gagggcccta ctggcgttcc tccaacacgc
     3361 ggctcgcaac ggggcgacga ggctcgccac gcccgtttcg acctcgaggg tgtgcgagga
     3421 cgtcgctgcc gaggcgggcg cgacgatcga gtggagcccg atctcaatgc cgttgctcat
     3481 ggaacgcgtc ggcgacggcc gggtgggctt cgcgggcacg cccgagggtg tattcatctg
     3541 gcccgagttc atgccggcgc cggatgcgct catgaccttc ggtaaagctt tagagctgat
     3601 cgcggcggcc cacaagccgc tggccgacat cgtggacgag ttgcccccca cccacgtcgc
     3661 gcgccggagc gtgcggaccc cgtggcagct gaagggaacg gtgatgagag agctcgcagc
     3721 cggccacggt gtgaccgatc cgtcgagccg gttggtgctg atcgatgggg tgaaggttct
     3781 caccggggat cgatgggtgc tggtggctcc ctatccggac gaaccgtcgt gcggcgtgtg
     3841 ggcagaggcc gccgatgccg ccgcgtcgga agagctcgcc gacgagtacg cagcccgtgt
     3901 ggaagagatc gtggccgccg gcgatccggg tcaacatctc gatttgtaga catgtcgaca
     3961 ctagggacgg aggacataag cgatcggctc ggcgatcgcg ccgtccgaag cgaagggctg
     4021 accgcgcagc gggcatacct gagggaggac atatgagcga ggttccggca gacctgaggt
     4081 acacgaagga gcacgagtgg gctcgccgcg acgacgatcg tgtcgtggtc gggatcaccg
     4141 actacgccca ggaccagttg ggcgacgtcg tgttcgtggg cctgcccgag gtgggtgccg
     4201 acgtcgaagc gggcaagccg ctgggggagg tcgagtcgac caagtccgtg tcggacgttt
     4261 acagcccgat cagcgggcgg atcgcggcga cgaaccccga ggtcgagcag aaccccgagc
     4321 tcgtcaacga ggagccctac gggcgggggt ggctggtggc gatcgaaggc ggcgatatcg
     4381 acggcctcat gagcgccgac gagtacaaag cgctgatcga aggcgcggcg agcgcgtgat
     4441 cggctcggcg atcgcgcaag cccgattgac caaccctaaa ggtttcctat agggtcagcc
     4501 gccatgtatt gcacgaactg cggacaccgc aaccccgagg gctccaactt ctgttcctcg
     4561 tgcggcgcgc ccttgaagga cctgaaagaa gggggagaca ccgacaccac gatcacgttc
     4621 atgcccggcg agctcgagtc cgacctcgac gaagatatcc atatctcgcc cgatgagctc
     4681 gaggggggcc ggggcgttct gatcgtcaag cgcggcccga acgccgggag caagttcttc
     4741 atcgacacga acgtgaccac cgtcggccgg catccggaat cggacatctt cctggacgac
     4801 atcacggtgt cccgcaggca cgccgagatc aggcggtcca acgggtcctt cggtctgcac
     4861 gacgtgggca gcctcaacgg cacttacgtg aaccgggaac gggtcgagga gacgggcctg
     4921 cactcgggtg acgagatcca gatcggcaag ttcaaactcg tgttcctatc cggaggttga
     4981 tccgatgcca ctatccagat cgcgcgacta cctgagcatc ggtgaggtcc tcgagaccct
     5041 ccaggaggag ttcccggacg tcaccatctc gaagatccgc ttcctcgagt ccgagggcct
     5101 gatcgagccc aagaggaccc cgtcgggcta ccgcatgttc ttcgacaagg acctgaaacg
     5161 cctccactgg atactcacga tgcagaggga ccacttcctg cccctgaagg tcatcaagga
     5221 gcggatcgaa agcggcgctg gtaacggcga agccctcctc gcgcccgcgg cccccccggc
     5281 gccggcgaag ggagccaagg gtgccaaggg cgccaaggcc gccccggtag cagctgctgc
     5341 accgcctcca cccgacctca cgacggtcca actgtctcgc cccgagctcc tcaagtcggc
     5401 gggcctgacg gagcgggagc tggcgagcct cgaggagtac ggcatcgtcg gaccggggga
     5461 ggggggcaac tacgacggcg cggccctcgc gatggctcgg gccgctaaac ggttcttcga
     5521 ctacggcgcc gaagcgagac acctaaagat gtaccggcag ttcgccgagc gcgaggtcgc
     5581 tttcttcgag cagatcgttc tgcccgtgtc gcgtaggcga gatcctgcgg cccagaaaga
     5641 cgccgctcgc tcggtgaggg aactggccgg cctcgcccgc cagatgcgcg acgccgcgct
     5701 ccacacgagc ttgcaagagc acctctagct tctgtggatt tgtcgacaag tcgactcctg
     5761 atcgggggcg tgctcgccgc gctcgtggca ctcccggccc ccgcgacggc aggtttcgtc
     5821 gccccccagc agtcggaccc cgtcgtctcc tgcaccgcct gcatcgttgt ggacgacacc
     5881 gggcgggtgc tgtgggcgcg cgccgcccat cgccgcctgc cgaacgcgag caccaccaag
     5941 atggtcaccg ctctggaggt cgtcgctcga tcacggcagt ccgaggtcgc cctggtctcg
     6001 gcggaggccg cctcggtcgg gggagggggg ctggatctcc aggcggggga cgagatgacg
     6061 gttcgcaacc tgctgttcgc gatgctcctg tcgtcctcga acgagtccgc ggcagccctc
     6121 gccgagcacg tggctcccag ccaggaggcc ttcgtcaggg ccatgaacgc cagggcacgg
     6181 ttcctgggca cgaccgacac gcatttcatg aatccgcacg gcctcgacgc gaccgggcac
     6241 tactcgtcgg ccgccgacct cgcgctgatc ggggccgcgg tcctcgatag gcctctgctc
     6301 gccgacatcg tggctacccc cagcacgtcg atcatgacgc cgcggggggc ggtggtcgag
     6361 gagaaccgta atgttctact cgagggttat ccaggagcga tcgggatcaa gaccggccgc
     6421 acgatcggag ccggcaacgt gctcgtggcc gccgcgacgc gaggggaccg gacggtgatc
     6481 gcggtggcga tgcattcgac cgatgcagcg aacgacgcgg cccaactgtt ggacttcgga
     6541 ttcgcgcgca tcgaacgact cgatacccgc gtgcccctca cgcttctgct cgcgaatacc
     6601 gacgtgggct cgatcgtctt cgatccttcg ggcgcaacct ccgtggttgc cgcgtcctcc
     6661 atccggctcg atcttccgcc cgggaccgat tcgttctcgt acacgctgcg accggcatcg
     6721 gtgacggcac cgctcgaggc tggagagcgg gtgggtacgg tttcggtcag tgtggatgga
     6781 cgagagctgg gcgaagtgga tgccgtggcg gtagacgacg tcgatcgaag cgagggtgat
     6841 ggactcgcga acgctctagg cacccttatg gcggcggttg caacgctggt gtcggcggtt
     6901 acgtgagcca gggcgccggc accgcacatc tcttgatcgc ggccggtgac atcgagcggc
     6961 gtgtaaccga gctcgcgcac gagatcacgg ccgacctggc cggggccgaa cccccactac
     7021 tcatcggagt gttaaaggga tgcacgctgt tcctggccga cctcgtgcga gcgatcgata
     7081 tcgacgtgca cgtcgacttc atagccatct cgtcgtatgc cggaggaacc gggagctcgg
     7141 gcgtggtgcg gatcctgaag gacgtggaag ccgatatccg tggccgagac gttctgatcg
     7201 tcgaggacat cgtcgacacc ggtctgacct tgacctacct gcgccgcaat ctcgaggaac
     7261 gcggagcgcg ttcgttgagg acggtcaccc tgctggacaa ggccgctcgt cggatagttc
     7321 ccgtagcggt ggaatacacg ggtttcgaga tcgaagacgt tttcgtggtg ggctacggcc
     7381 tcgatttcca gggccgctat cgcaacgtca ccgacgtcct ggcggtgacc gatatcgccc
     7441 ggctggccaa cgatccgctg ctgctcgttg agcccttgtg gccagcggtt aagagatagg
     7501 ggggtgttag tttcgttgcc atgatcgagc tctcgttggt gggcgtgaga gtcgagcttc
     7561 cgtccaatca acccatcgtt ctgctcaagg agacgggagg ggaccgttat ctcccgatct
     7621 ggatcggcgc cgtcgaagcc accgcgatcg cattcgccct ccaggggatc gagacgcccc
     7681 gcccgatgac tcacgacctg atgcgagaca tcctggccga gtcacagatc caggtcgatc
     7741 gcatcgtgat ctcagacctc gtcgatcaga ccttctacgc gaccatcagc ttgagcatga
     7801 acggacgcgc catggaagtg tcgtcacgac cgagtgacgc gatcgccctt gcggttcgcc
     7861 tcgcgactcc gatcttcgcc gcggaggagg tcatcgagca ggccggcatc gagctgcgcg
     7921 acgacgagga gaccgaggtg cagaaattca gggagttcct cgaccaggtc agccccgaag
     7981 acttcgccgc gggctagacc gggaagagag aaaccgcgat cggctcggcg atcgcgggga
     8041 acaaaccgcg atcggctcgg cgatcgcggg gaacaaaccg gacccctcta gttctgcttg
     8101 acggttttcg ttgacaacgg cgcgcggccc ccgtacgatt tccaccctcg taactacgcg
     8161 agtgtgactc gcgccggggc cgcagctagg gcctgtaatc gagacgagaa ggcgatcggc
     8221 tcggcgatcg ccagagcaaa ggggaggatt catggaccag gcactcgagg ttggttaccg
     8281 cggtcctcaa gcctgcaaga tcgtcgacat cacctatcgc cagctcgact actggacccg
     8341 gacggggctg gtcagcccct cgatccagcc ggccaagggc tccgggaccc agcgcctcta
     8401 ctcgttcaac gacctcttgc agctgaaggt gatcaagagt ctcgtcgacg ccggggcgag
     8461 cctccagaag gtccgtcagg cgatcgacta cgtccgcgaa gaactcgacg ccgattgggc
     8521 caaggtcacg atcgtggccg acggatcggg tgtctacgcg tgcacctcgg atgccgaggt
     8581 cgtcgacctg cttcgttccg gccagggtgt cctgggcgcg atcgttgcgg tcgggaaggt
     8641 gcgcgatcag ctcgccggta cgttgcgcga actccgcccc ctggaacgct ccgtggagta
     8701 cggtgatgcg aaggaccctg atgggtccgc accgaccacc acgccgggat cgagggcagg
     8761 ggagggctaa gagatcgtcg aggccacaga cgctcgagag catctgacgg gccggcttct
     8821 tccccacgag cgcgtcgaat tcgcacataa cagcgagcgc cagttcgcga agcttctcga
     8881 cttctacgag atcgagtggg cgtacgagcc cacgaccttc gacatcgaat gggacggcaa
     8941 cggccgtgcc acccagcggt tctcgccgga cttctatctc ccgaccttcg acctttacat
     9001 cgagatcacg acgctcaacc agaagctggt gaccaagaag aaccgcaagg tccgccggct
     9061 catgcagctg tacccggacg tcaagtgcaa ggtcctctac cagagggact acctgaacct
     9121 gttggtgaag tacggcctcg aagagccctc tcagggcccc cttatagagg ccgacgccaa
     9181 cagcggcccg gccgaggtcc acaaggtcct gctcggcccc cggctcgcgg gctgatcgag
     9241 cgtggagggg accgcccgcg cgaccgtcct cctggaagag caccggaagc tcggagccaa
     9301 gctcaccgag ttcggcggct gggagatgcc ccttcaatac accgggatcg tcgcggagca
     9361 ccgcgccgtc agggagacct gcgggatctt cgacgtgtcg catctcggca agctgcgagt
     9421 taccggacgc gacggggggt ccgctctgca ggaggccgtc accgccgacg tcgagtcgct
     9481 cgaggtgggg cgcgcgtctt atgccctggt cctgaccgaa gaggcggggt gtgtggacga
     9541 cgtcttcgtt tatcgcatcg gcgaacagga gtggctgtgc gtaccgaacg catcgaacgt
     9601 cgctgccgtc gccgacatga tctcggcggg cggagccgat gcgcaggacg agtgggaccg
     9661 ctggacgatc ctcgcgatcc agggccccga ttcgttctcg ctcttcgagg aggtcttcgg
     9721 tagatcggac gcgctcgaac tcgcactgca ctctttctgt ccactggacg tcttcggatc
     9781 ggtcgggatc gtggcgcgca ccggatacac gggggagcgc ggattcgagc tctacgctcc
     9841 ggtcgacacg gcggtgaagg ccttctcgag cttgctcgaa gccggaggga cgccggttgg
     9901 actcggagcg cgcgacacgc tccggctcga gatgggctac gcgctctacg ggcacgagct
     9961 gaccctggac acgaaccccc tcgaagccgg tctcggctgg gcggtcgact gggacgcacc
    10021 gtttcggggt cgcgatgcac tggcttccat caaggagacc gggccccgcc gacgcttgat
    10081 ggggatcgtc tgccgggacc ggggcgtccc gcgtcagggg taccgcgttc tcagcggcga
    10141 gaagcagatc ggggaagtgg tcagcggcaa tcactcgccg acactcgggg tagggatcgc
    10201 gctcgcgtac ttccccgtcg aggagttgcc gcagccgggc gaggaggtcg cggtggaagc
    10261 gcgcggccgc agcatcgtcg gggatatcgt gaaacctcca ttcatcaaga agagatagac
    10321 atgtcgacaa atagacaatc gagctaagcg tggacttcct cccacacacc gacgcagacg
    10381 tcgccgacat gctggccgtc atcggcgcgg cctcgatcga ggagctcttc gagcccgtac
    10441 cggccgggat ccggctggat cgctcgctcg atgtcccgac tgcgctcagt gaacccgagt
    10501 tgctcagaca cctccgcgag ctggcgagcg cgtcgtccgg cgcaaccgac ctcgtctgct
    10561 tcgcaggggg aggggcctac gaccaccacg ttccgtccgc ggtcaaggcg ctggcgagcc
    10621 gggccgagtt cgcgacctcc tatacgccct atcagcccga gctgtcgcaa ggagtgctcc
    10681 aggcggtctt cgagttccag acgctcgtat gcgagatctt cggtctcgag gtggccaacg
    10741 cgtccctcta cgacgg
//
LOCUS       DATMED010000332         4201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3353742, whole genome shotgun sequence.
ACCESSION   DATMED010000332 DATMED010000000
VERSION     DATMED010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3353742"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..2427
                     /locus_tag="VNP73_08575"
     CDS             37..2427
                     /locus_tag="VNP73_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWL66014.1"
                     /translation="MSTAPTTDPQTPARHAADSHDMIRVLGARVNNLKDVSVEIPKRR
                     LTVFTGVSGSGKSSLVFDTIAAESQRMINETYSAFLQGFMQNQSRPDVDVLDGLTTAI
                     IVDQERMGANPRSTVGTATDANAMLRILFSRLGKPHIGPPTAYSFNVPKRTASGAMTV
                     DKGKGERVVVRNAVYHGGMCPRCEGMGSVTDFDLSQVYDDSKSLNEGALTIPGYSMDG
                     WYGRIFRGSGFFDPDKPIGKYTKKQMHDLLYKEPTKIKVEGINLTYEGLIPRIQKSML
                     SKDVDALQPHIRAFVERAVTFTTCPDCGGTRLSEEARASKIKGKNIADVCSMQLGDLA
                     DWLRSLDKPSVAPLLTGLQHLLDSFAEIGLGYLSLDRPSGTLSGGEAQRTKMIRHLGS
                     SLTDVTYVFDEPTIGLHPHDVQRMNELLLRLRDKGNTVLVVEHEPETIAIADHVIDLG
                     PGAGAAGGEVVFEGTVDGLQDSGTLTGRHLGDQVSLKPSVRTPSGSLEVRGADTHNLR
                     DVDVDIPLGVLVVLTGVAGSGKSSLIDGSVAGREGVVAIDQIAIRGSRRSNPATYTGL
                     LDPIRKAFAKANGVKPGLFSSNSEGACPTCNGAGIIYTDIGIMATVESTCEECEGKRF
                     QASVLEYTFGGRNIAEVLAMSVTDAERFFGAGEAKTPAAHKILTYLADVGLGYLSLGQ
                     PLTTLSGGERQRLKLATHMGEKGGAYVLDEPTTGLHLADVEHLLGLLDQLVEDGKSVI
                     VIEHHQGVMAHADWIIDLGPGAGHDGGRIVFEGTPADLVGDGSTLTGKHLASYVSA"
     gene            complement(2454..2777)
                     /locus_tag="VNP73_08580"
     CDS             complement(2454..2777)
                     /locus_tag="VNP73_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66015.1"
                     /translation="MAWEEYMVAIRRFTSALTMTGSWKNPKFLRVERRFDGRTKQPQC
                     FIFADADGLITPTVFFFPHNRHPDMLPLTMDAMGTKEYEDVEALVDDGWSPFSDDDLN
                     LGLSF"
     gene            2951..3421
                     /locus_tag="VNP73_08585"
     CDS             2951..3421
                     /locus_tag="VNP73_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66016.1"
                     /translation="MGSKLRASSPVIALVLVLVGAMLVAPASAGKKKRVERVDEREYI
                     LATGPRGAADTPCVAAPIGCVIFPLEKGDKFVDVEVIDDAGEPVWASVYVYGYTDGSD
                     THEHICGESEAPLKLAPDMEELVVVTTQTTGGATSPCTGPATQGTIKVTFSNLP"
     gene            complement(3408..3695)
                     /locus_tag="VNP73_08590"
     CDS             complement(3408..3695)
                     /locus_tag="VNP73_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66017.1"
                     /translation="MEQIDFLVDSTLGEIEVHLNRDGWASFGLQETDDPLLVEDMVRK
                     ATDVARCLTTVGLPAEEASELAAELWDELDEGEQLERARIPGTSSPDTTEG"
     gene            3723..3796
                     /locus_tag="VNP73_08595"
     tRNA            3723..3796
                     /locus_tag="VNP73_08595"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3757..3759,aa:Ile,seq:cat)
BASE COUNT          779 a         1293 c         1377 g          752 t
ORIGIN      
        1 cgcgccgggc ggtcccgccg caccgaggga gacccgatga gcacggctcc gacgaccgac
       61 ccgcagacgc ccgcgcggca cgccgccgac agccacgaca tgatccgcgt actcggcgcc
      121 cgggtgaaca acctcaagga cgtcagcgtc gagatcccca aacgccgatt gaccgtattc
      181 accggcgtct ccggctcggg caagagctct ctggtcttcg acacgatcgc cgccgagtcg
      241 caacggatga tcaacgagac ctacagcgct ttcttgcagg gcttcatgca gaatcagtcg
      301 cggccggacg tggacgtgct ggatggcctg acgacggcga tcatcgtgga ccaggagcgg
      361 atgggggcca acccgcgctc caccgtcggc actgccaccg acgccaacgc gatgttgcgc
      421 atcctcttca gccgcttggg gaaaccgcac atcgggccgc cgactgctta ctcgttcaac
      481 gtcccgaagc gaacggcgag cggtgcgatg accgtcgaca agggcaaagg ggagcgggtc
      541 gtggtgcgca acgccgtcta ccacggcggg atgtgtccgc gctgcgaagg tatgggctcg
      601 gtgaccgact tcgatctgtc tcaggtgtac gacgacagca agtcgcttaa cgaaggcgcg
      661 ctcacgatcc ccggctacag catggatggc tggtacggac gcatcttccg cggcagcggc
      721 ttcttcgacc cggacaagcc gatcgggaaa tacaccaaga agcagatgca cgacctgctc
      781 tacaaggagc cgaccaagat caaggtcgag gggatcaacc tgacctacga ggggctgatc
      841 ccaaggatcc agaagtcgat gctctccaag gatgtcgacg cgctgcagcc gcacatccgg
      901 gcgttcgtgg agagggccgt gacgttcacg acctgtcccg actgcggcgg tacccggctc
      961 agcgaggagg ctcgggcgtc gaagatcaag ggcaagaaca tcgccgacgt ctgctcgatg
     1021 cagctcggcg atctcgccga ctggctcagg agcctcgaca agccgtccgt ggccccgttg
     1081 ctcaccgggc tgcagcatct cctcgactca tttgcggaga tcggactggg atacctctcg
     1141 ctcgaccggc cttcgggcac actgtccggg ggagaggcac agcgcacgaa gatgattcga
     1201 cacctcggct cctcgctcac cgacgttacc tacgtcttcg acgaacccac catcgggctg
     1261 cacccgcacg acgtccagcg gatgaacgag ttgctgctgc ggctgcgaga caaggggaac
     1321 accgttctcg tcgtggagca cgagcctgag acgatcgcga tcgcggacca cgtcatcgac
     1381 ctcggccccg gcgcgggcgc tgccggtggc gaggtggtgt tcgaaggaac cgtcgacgga
     1441 ctgcaggaca gcggcaccct gaccgggcgg cacttgggcg atcaggtttc tttgaagccg
     1501 tctgtgcgga cgccgtcggg atcgctcgag gtgcgcggag cagacaccca caacctgcgt
     1561 gacgtcgatg tcgacatccc gctcggtgtg ctggtcgttt tgaccggcgt ggccggatcg
     1621 ggcaagagct cgctgatcga cggctctgtg gccggtcggg aaggggtggt ggcgatcgac
     1681 cagatcgcga tccgtggctc gcgacggagc aacccggcga cttacaccgg actgctcgac
     1741 ccgatccgca aggcgttcgc gaaggccaac ggcgttaagc cgggactctt cagttccaat
     1801 tccgagggcg cttgcccgac ctgcaacggc gcaggcatca tctacaccga catcggcatc
     1861 atggctaccg tcgaatccac ctgcgaggag tgcgagggga agcggttcca agcgtcggtg
     1921 ctcgagtaca ccttcggcgg ccggaacatc gccgaagtgc tcgccatgtc ggtgaccgat
     1981 gccgagaggt tcttcggggc cggcgaagcg aagacgccgg cggcgcacaa gatcctcact
     2041 tatctggcgg acgtgggact cggctacctc agcctcggcc agcctctaac gactctgtcg
     2101 ggcggcgaac gacagcggct caaactggcc acccacatgg gcgagaaggg gggcgcctac
     2161 gtcctcgacg agccgaccac cggcctccac ctagccgacg tcgagcacct actcggtctg
     2221 ctcgaccagc tcgtagagga cggtaagtcc gtcatcgtca tcgagcacca tcaaggggtc
     2281 atggcgcatg cggattggat catcgatctc gggcccggcg cgggccacga tggcggccgc
     2341 atcgtcttcg agggaacacc tgccgatctc gtcggcgacg gctcaaccct caccgggaag
     2401 catctcgcgt cttacgtgag cgcgtgaccg atctggatcc agctaagcgt ggatcagaaa
     2461 ctcaagccta gattgaggtc gtcgtcgctg aaggggctcc acccatcatc gacgagtgcc
     2521 tcgacatcct cgtattcctt cgtccccatc gcgtccatag tgagaggaag catgtctgga
     2581 tggcggttgt gagggaagaa gaaaacggtg ggggttatga ggccgtcggc atcggcgaag
     2641 atgaagcatt gcggttgttt cgtccggccg tcgaaccttc tctcgacacg caggaacttc
     2701 gggttcttcc atgagcccgt catcgtgaga gctgatgtga atcgtctgat tgcgaccatg
     2761 tattcctccc aagccactga ttacagtaga gatatcggcc ggatccggac ccacatttag
     2821 ggcaaggcgg cctcgggccg gatatttggt cccataggac tagtaccccc aggcaatagg
     2881 cgtcgggccg gcagggtcac agtggaggca cggcgaatcc ttcagtcacg tctaggtaaa
     2941 ggggttcgcg gtgggatcga agctgagggc gtccagcccg gtcatagccc tagtgctggt
     3001 cttggtgggg gcgatgctcg ttgctccggc ttcggccggc aagaagaaga gggtcgaacg
     3061 cgtcgacgag cgtgagtaca tcctcgccac cggcccccga ggggcggccg acacaccttg
     3121 cgttgccgcg ccgatcggat gcgtgatctt tcctctcgaa aagggcgaca agttcgtcga
     3181 cgtcgaggtg atcgacgacg ccggcgagcc ggtgtgggcg tcggtctacg tgtacggcta
     3241 caccgacgga agcgatactc acgagcacat ctgcggagaa tcggaagcac ctttgaagct
     3301 tgcaccggac atggaggaac tcgtggtcgt aacgacgcag acgacgggag gggcgacgag
     3361 tccttgtacc ggcccggcaa cgcagggcac gatcaaggtg actttctcta accttccgta
     3421 gtgtccgggc tgctggttcc ggggatccgt gcccgttcga gctgctcgcc ttcgtcgagc
     3481 tcgtcccaca attcggccgc gagttcgctt gcctcttcgg ccgggaggcc gaccgtggtc
     3541 agacaccttg cgacgtcggt cgccttcctg accatgtctt ctacgaggag gggatcatcg
     3601 gtttcctgta gcccgaagga ggcccagccg tctcggttca ggtgcacttc gatctcgccc
     3661 agggtcgagt ccaccaggaa atcgatctgt tccatgtgcc tcgcatcgta gggtcctgct
     3721 cgggggccgt agctcagttg gtcagagcgg cggactcata atccgttggt cgctggttcg
     3781 agtccagccg gccccacttg cgaagctgct ggtcagaggc agctttcgcc ttcttctcgt
     3841 ctaggtcgat gaccgtttca tctttcgtag gccgcggata ggccgcggct cgttgagcgt
     3901 cgaggcctcg agcgacctca tccatcgcat ccgggaacag gtgtccgtag cgatcgaggg
     3961 tcactgtgat ggatgagtgg ccgaggtgag cctggatcgc tttcgggtgg gttccttggg
     4021 caataagcag cgacgcgcac gtgtggcgca ggtcgtggat gcgcaggtcc tcggggagcc
     4081 tggcctcgcg gagcgcgggc agccagactc ggttgcggaa gttggagttg ctgagcggct
     4141 gaccgttccg agatgtgaac acaagcgcat cagggtcttg ggggagcgca ctgaggtgct
     4201 c
//
LOCUS       DATMED010000333         6030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_284197, whole genome shotgun sequence.
ACCESSION   DATMED010000333 DATMED010000000
VERSION     DATMED010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284197"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VNP73_08600"
     CDS             complement(<1..668)
                     /locus_tag="VNP73_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HWL66018.1"
                     /translation="MTAKIIDGKAIAADVRAEVAQEVKELANRDVVPGLATVLVGDDP
                     ASKIYVNNKRKMCAEIGIKAWDHDLPASTPEDELLDLIRRLNDDGEVHGILVQVPLPE
                     HIDEQVVLDLVDPNKDADGFHPCNQGRLLAGNRLVASATPAGIQELLIRSGVEIAGAE
                     VVVVGRSNIVGKPIAAMLMQKGHAANATVTVCHTGTRDLEAHTRRADILIAAIGRAHA
                     ITGP"
     gene            724..2043
                     /locus_tag="VNP73_08605"
     CDS             724..2043
                     /locus_tag="VNP73_08605"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL66019.1"
                     /translation="MSEKLVTPPTTPPPQGTHSEPALMRIGMIRPLRHRDFRYMWVGL
                     TVSLFGDGLYLVAIAWQTYQIRNTASALGIVLTAWSLPMVLALPFGGMVSDRFDRRKV
                     LIASDVIRGVAITIMGVLSLSGIIEIWHLVILAGFYGIGQAFFGPAFGAIVPDLVPQD
                     ELVQANALDNFVRPMAERFIGPAVGGLIAEFWSPGGAFVLDGFTFAVSALMIMRMTHR
                     PGARRADGSTAWSEVKEGFAFVRSKTWLWATLACASVSLLFVFGPFELLVPLLIKNRL
                     GGGADDVGYVFAASGAGALLAAFLMGQYGMPKRHIVFLYVGLALGIFLMWPYAFVTAT
                     WQAAVIEFFAWGAWSASLVVWTTLMHRLVPRELLGRVTSLDWMVSISLTPISYALTGP
                     ISDSIGLDATFIWSGVLGGGLCIAFLFVPGVRASERDGSIHPPTVLS"
     gene            complement(2087..2611)
                     /locus_tag="VNP73_08610"
     CDS             complement(2087..2611)
                     /locus_tag="VNP73_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL66020.1"
                     /translation="MIVRPATANDIRGITRVHKEGWRAGYADLFPPEVLARAIEKHCT
                     RWPRVFASDDFAETTMLVAEQDGSILGFAHLGPGEHDAGRFEDSEPGRVELYSFYVDP
                     SYWGSGIAGELLKSVLAELQTQGHREVFLSTYAGVSRARAFYTKSGFHETGRTSVYDF
                     EDVPVPEVEYSRTI"
     gene            complement(2730..4256)
                     /gene="purH"
                     /locus_tag="VNP73_08615"
     CDS             complement(2730..4256)
                     /gene="purH"
                     /locus_tag="VNP73_08615"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWL66021.1"
                     /translation="MKIERALVSVSDKTGLAAFAQGLVDAGVEIVSSGGTASSLRDEG
                     IPVTAVADVTGAAEMLDGRVKTLHPNIHGAILADRRKPEHLEQLKERGISPIDLVVCN
                     LYPFERTIADGASEDEAIEQIDIGGPAMVRAAAKNFHSVAVVVKPERYEEMLEEIRSS
                     GEISDSTRRRLSLEAFQHTAAYDSAIAGYLAGEDSFPETFLFVGDKALDLRYGENPHQ
                     SAAFFRSRGVVTGLAAAEQLHGKDLSYNNLLDTDGAWKLVLELPRPSAAIIKHSNPCG
                     VAFGSSLEDAYARALDCDRTSAFGGIVALNESCDEATARAIGEIFTEVVIAPDFDEGA
                     LGILTEKKNLRIMRAEPSLLPEADLRKVSGGLLVQSPDPPDPPDLAEDAKVVTKVQPT
                     QEQWDDLRFAWVVVKHVKSNAIVLVKDRVAVGVGAGQMSRVESTELAARRAGDRAKGT
                     ACASDAFFPFRDGLDAAVAAGAAAVIQPGGSVRDEEVIAAADEHGIPMVFTGRRHFRH
                     "
     gene            complement(4274..5218)
                     /locus_tag="VNP73_08620"
     CDS             complement(4274..5218)
                     /locus_tag="VNP73_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL66022.1"
                     /translation="MIPLMTSELKMRLRGRRWWLLLLLWVAVLFLLLLLVWKVTQPIS
                     FGPTRDPIGPIMFGSLMMLVLGFAALIVPSLTATSINGEREKGTLAVLQATLLRPHQI
                     VLAKFGAAWITSLAFVVATLPLTLWCILEGGVGLLRVAAVYLVLVVTTAFLVSIGLGV
                     SSFIKRSALSAAATYGVVGTLIFGTLFFFGISMAGAPQREVRQQLPDGTEYVTFEPVI
                     GQRWLWLAPNPFVVLADAAPGTSPQGDGDPLTWISEAARSTRAPSGRFLTDPGRSGPP
                     LWPAGIAIYAALALISVLVARGRLRVPARKLVPGERIA"
     gene            complement(5215..>6030)
                     /locus_tag="VNP73_08625"
     CDS             complement(5215..>6030)
                     /locus_tag="VNP73_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL66023.1"
                     /translation="GPNGAGKTTLLLILAGLLAPDSGEASVAGFDPVTQPYEVHRVVG
                     WMPDFFGVYDGLTSREYLDLFGATFSMTLNERSIRVEELVADLDLGTFADNPIHTLSR
                     GQKQRLGFARAIVHRPSLLLLDEPASGLDPRARIDLRDLLLRLKGEGVSIVISSHILS
                     ELEEMADDVAFVDAGRSRGLFPLSDLPRGAALASWRISALQLEPLVAALQSNGTPFTQ
                     LVGASVEVHLENEQMAAALITQLVGQGVQVTEVIRGGSGLEQAFMALESEAAG"
BASE COUNT         1115 a         1933 c         1848 g         1134 t
ORIGIN      
        1 atgggtcccg ttatcgcgtg ggctctgcct atcgcggcga tcaggatgtc ggctcgcctc
       61 gtgtgcgcct cgagatctct cgtacctgtg tgacagaccg tgacggtcgc attagcagcg
      121 tgacccttct gcatcaacat cgcagcgatc ggcttcccga cgatgttgga ccggccgacc
      181 accacgacct cggccccggc gatctcaacg cctgaacgga taaggagctc ctggatgccc
      241 gctggggtcg ccgacgcaac gagcctgttg ccggcaagca gccggccctg attgcaaggg
      301 tggaaaccgt cggcatcctt gttcgggtcg accagatcga gaaccacttg ctcgtcgatg
      361 tgttcgggca ggggaacctg aacgaggatc ccgtgaactt caccatcgtc gttaagccgt
      421 cggatcagat cgagtagttc gtcttcgggc gtgctcgccg gcaggtcgtg atcccacgcc
      481 ttgataccga tctccgcaca catcttgcgc ttgttattca cgtagatctt cgaagccgga
      541 tcgtcgccca ccagaacggt cgcgaggccg gggacgacgt cgcggttagc cagttccttc
      601 acttcctggg cgacctctgc gcgaacatcg gctgcgatcg ctttcccgtc gatgatcttc
      661 gcggtcattt gtcccccttt tcgctagacc gtcgcatcct actgagcctt cagaatggac
      721 aaaatgagcg agaaactggt aactccacca acgactcccc caccgcaggg aacacatagc
      781 gaacccgcat tgatgcggat cgggatgatc cgccctcttc gccatcgcga cttccgctac
      841 atgtgggtgg gcctcaccgt gtccttgttc ggcgacggct tgtatctcgt cgcgatcgca
      901 tggcagacct atcagattcg caacaccgcg agtgctctgg ggatcgtcct aacggcatgg
      961 agccttccga tggtcctagc cttgcccttc ggcgggatgg tgagcgaccg gttcgaccgc
     1021 aggaaggtgc tgatcgcgtc ggacgtgatc cggggtgtcg cgatcaccat catgggtgtc
     1081 ttgtcactgt cgggcatcat cgagatctgg cacctcgtga tcctggccgg cttctacggg
     1141 ataggccaag cgttcttcgg cccggcgttc ggtgcgatcg ttcccgatct cgtgccccaa
     1201 gacgaattgg tgcaggcgaa cgcgctcgac aacttcgtga ggccgatggc ggagcgtttc
     1261 atcgggcccg ccgtcggagg cttgatcgcg gagttctgga gtccgggcgg agccttcgtg
     1321 ctcgacggat tcacgttcgc tgtctcggcc ttgatgatca tgcggatgac ccaccggccc
     1381 ggagcgcggc gggcggacgg ctcgacggcg tggtcggagg ttaaagaggg cttcgcgttc
     1441 gtacggtcta agacgtggct ttgggcgaca cttgcttgtg cttcggtctc actcttgttt
     1501 gtcttcggtc ccttcgagct cctcgtgccg ctactgatca agaaccggct ggggggcggc
     1561 gcggacgatg tggggtacgt gttcgcggcg agtggagcgg gagcactcct ggccgcgttc
     1621 ctgatgggtc aatacgggat gcccaagcga cacatcgtgt tcctttacgt cgggttggca
     1681 ctggggatct tcctcatgtg gccgtatgcg ttcgtcaccg cgacctggca agcagcagtg
     1741 atcgagttct tcgcctgggg ggcctggtcc gcctcactcg tggtctggac gacgttgatg
     1801 catcgtctgg ttccgcgcga gctgctgggg agagtcacga gcctcgactg gatggtgtcg
     1861 atctctttga cgcccatctc gtatgccttg accggaccga tctccgattc catcggcctg
     1921 gatgcaacct tcatctggtc cggagtactc ggcggaggcc tgtgcatcgc gttcctgttc
     1981 gtccccggag taagagccag cgagcgagac ggctcgatcc acccccccac cgttctttct
     2041 taacattccg cccccttttc gggcctgcga gtcagaaaag aaaaggtcag atggttctcg
     2101 agtactcaac ctccggaaca ggcacgtcct cgaagtcata aacgctcgtt cgacccgtct
     2161 catggaaacc tgacttggtg tagaacgctc gcgcacgaga gacccctgca taggttgaga
     2221 gaaatacctc gcggtggccc tgggtttgga gttcggctag gacgctcttc agaagctcac
     2281 ctgcgatccc cgaaccccaa tacgaggggt caacatagaa actgtagagc tcgactcgtc
     2341 cgggctcgct gtcttcgaac cggcccgcgt catgctcccc cgggccgagg tgggcgaacc
     2401 cgaggatgga tccatcctgt tcggccacga gcatggtggt ttctgcaaag tcatcgctcg
     2461 cgaagacgcg tggccacctc gtgcaatgtt tctcgatagc tctggcgagg acctccggcg
     2521 gaaagaggtc ggcataaccg gctctccagc cctccttatg gactcttgtt atccctcgga
     2581 tgtcatttgc cgtcgccggt cggacgatca tctcgatctc ctactccgtt cttttctaag
     2641 ttcgccgccc aaaacagggg ctggatgtgc agaaagatcg gagcgggcta cgcccgcaca
     2701 atcagaacca cccgcccgct gcccaacggt taatgacgga agtggcggcg gccggtgaag
     2761 accatcggga tgccgtgctc gtcggccgcg gcgatcactt cttcgtccct cacggaaccg
     2821 ccgggttgga tcacggccgc ggcccccgcc gctaccgcgg catcgagccc gtcgcggaac
     2881 gggaagaagg cgtctgaagc acacgcggtg cccttagctc ggtcacccgc acggcgcgca
     2941 gccagctcgg tcgactcgac cctgctcatc tggccggccc cgacgccgac ggcgacgcgg
     3001 tccttgacca gcacgatcgc gttcgacttc acgtgcttca cgacaaccca cgcgaaacgg
     3061 aggtcgtccc actgctcttg agttggctga accttcgtca cgactttggc gtcctcggcg
     3121 aggtcgggtg gatcgggtgg atcgggtgac tgcaccagta gcccacccga caccttgcgc
     3181 agatcggcct ccggcagaag ggaaggctcg gcccgcatga tccgcaggtt cttcttctcg
     3241 gtcaggatgc ccagggcacc ctcgtcgaag tccggcgcga tcacgacctc ggtgaagatc
     3301 tcgcctatcg cccgggcagt ggcttcgtcg caggactcgt tcagagcaac gatgccgccg
     3361 aaagccgaag tgcggtcgca atcgagcgcc cgtgcgtacg cgtcctcgag cgacgaaccg
     3421 aacgccaccc cgcatgggtt cgagtgcttg atgatcgcgg cgctcggcct aggaagttcc
     3481 aaaactagct tccaggctcc gtcggtatcg agcaggttgt tatacgaaag gtccttgccg
     3541 tgaagttgct cggccgcggc cagcccggta acgacaccgc ggctgcgaaa gaaagcagcg
     3601 ctctgatgcg gattctcgcc gtagcggaga tcgagagcct tgtcgcccac gaacaggaac
     3661 gtctcgggga acgagtcctc gccggccaga taccccgcaa tcgccgagtc gtaagcagcg
     3721 gtgtgctgga acgcctcgag cgagagacgc cgccgggtcg aatcggagat ctcgcccgac
     3781 gaacggatct cttcgagcat ctcttcgtag cgttcgggct tcacgactac cgcgaccgag
     3841 tggaagttct tggccgccgc ccgaaccatc gccggccccc cgatatcgat ctgctcgatc
     3901 gcctcatcct ccgacgcccc gtcggcgatc gttcgctcga atggatacag gttgcacacg
     3961 acgagatcga tcggcgagat accgcgctct ttcagttgct cgaggtgttc tggcttgcga
     4021 cgatcggcca ggatcgctcc gtggatgttg gggtggagag tcttcacgcg accgtcgagc
     4081 atctcggccg caccagtcac gtcggcaacc gccgtcactg ggatgccctc gtcgcgaaga
     4141 gatgaagcag tacctcccga ggagacgatc tcgaccccgg cgtcgacgag accttgcgcg
     4201 aacgcggcca acccggtctt gtcgcttact gagacgaggg cccgctcgat cttcacggct
     4261 gcatcctaag tagtcacgcg atccgttcgc cgggcacgag tttgcgggcg ggaacgcgca
     4321 gtcgcccccg agcaaccagc accgagatca aagcgagcgc ggcatagatc gcgatcccgg
     4381 ccggccacaa ggggggcccg gaccgacccg ggtcggtgag gaatctgccg gagggagctc
     4441 tcgtcgatcg cgcggcttcg cttatccacg tcaatggatc gccgtcacct tggggcgacg
     4501 tgcccggcgc cgcatcggcc agcacgacga acggattcgg cgccagccac agccatctct
     4561 gtccgatgac cggctcgaac gtgacgtact cggtgccgtc gggaagctgt tgtctgacct
     4621 ctctctgtgg cgcgcccgcc atcgagatac cgaagaagaa cagggttccg aagatcaggg
     4681 tgcccaccac tccataggtt gccgccgcgg acaacgcaga acgcttgatg aacgaggaaa
     4741 cacccaaacc gatcgatacc aggaaagccg tggttactac gagaaccagg taaacggcgg
     4801 ccacgcgcaa caggccgacc ccgccttcga ggatgcacca caacgtcaac gggagagttg
     4861 ctacaacgaa cgccagtgag gtgatccagg ccgccccgaa tttcgccagc acgatctggt
     4921 gcggccggag gagcgttgct tgcagcacgg caagcgttcc cttttccctc tctccattga
     4981 tcgaggtcgc ggtcagggac ggcacgatga gcgcggcgaa ccccaacacc agcatcatca
     5041 aggaaccgaa catgatcggg ccgatcgggt cccgggttgg cccgaagctg atcggttgtg
     5101 tgaccttcca gaccagcaaa aggagaagga acaacacagc cacccacagc aacagcagga
     5161 gccaccagcg ccgccctcgc agccgcatct tcaactcgct cgtcatgaga gggatcatcc
     5221 ggccgcctcc gattcgagtg ccatgaaggc ctgctccaaa ccggatccac cccggattac
     5281 ctcggttacc tgcacgcctt ggccgaccag ctgcgtgatc agcgcggcgg ccatctgctc
     5341 gttctcgagg tggacctcga cggacgcacc caccagctgc gtgaacggtg ttccgttcga
     5401 ctgaagtgcc gccaccagag gttctaactg gagtgcggag atccgccacg acgccagcgc
     5461 agcgcctcga ggaagatccg acagggggaa cagcccacgt gaacggccgg cgtcgacgaa
     5521 cgcgacgtcg tcggccatct cctcgagctc gctgagtatg tgagacgaga tgactatcga
     5581 gacgccctct cccttgagcc tcaggagcag gtcgcgcaga tcgatccgtg ccctgggatc
     5641 gagcccgctg gcaggctcgt cgagcagcaa gagcgacggc cggtggacga tcgcccgagc
     5701 gaaaccgaga cgctgcttct ggccccggga caacgtatgg atcgggttat ccgcaaaggt
     5761 gcccagatcc aggtcggcta ccagttcctc gacccggatc gaacgctcgt taagggtcat
     5821 cgagaacgtc gctccgaaga gatcgaggta ctcgcgcgag gtgaggccgt cataaacgcc
     5881 gaagaagtcc ggcatccaac cgacgacgcg atggacctcg tagggctggg ttaccggatc
     5941 gaacccggcc acgctcgcct cacccgagtc cggcgccagg agtccggcga gtatcaggag
     6001 caaggtcgtc ttgccggctc cgttcgggcc
//
LOCUS       DATMED010000334         3632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4878065, whole genome shotgun sequence.
ACCESSION   DATMED010000334 DATMED010000000
VERSION     DATMED010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4878065"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VNP73_08630"
     CDS             <1..775
                     /locus_tag="VNP73_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWL66024.1"
                     /translation="KPDVVAPGTHTISLRSPGSAIDQEYGSTAVVQDDYFRGTGTSMA
                     AATTSGIVAQILQKTPSLTPDQVKNRLVSTARRIAVTDKYSVGKGLVDAYAAAKSTST
                     AKANQGLLYGLGGLSTGLGLIGPDRGSLGLGVVTPQGEGDLAGENKAQHQASLVSLSN
                     PLGLLPWTSLVYTTVGWDPVTWLTTSFATTDWSGVRWKETSWDGVRWKETSWDGVRWK
                     GTAWENSDWEGVRWKGTEWDGVRWKATSWQTRWYAAAWK"
     gene            811..3525
                     /locus_tag="VNP73_08635"
     CDS             811..3525
                     /locus_tag="VNP73_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL66025.1"
                     /translation="MTEERALPARFYVFVSVLWCGLITAFVLALRTDPAMGTHLWTMV
                     AFIALFATAELLDLSYHEKDATLGLSQCESILLPMIVALSFGEVLVAAVAGMGLARAF
                     DRSTNLGKQVFNLAQYGCAAAAASFLYVWLTVESGRFSIAEAGAATIAVIVFEVLTHS
                     FVAAALHVAGRGRFMDAFTDVAPVTFINLAGNVILGLLLAAAFSAEAWTIALFPLASI
                     ALFLGYRAVVRQQAERRRMVRLHEASRALAAAPDLGRALDGFLKAVAKTASALGARAV
                     VRMNDGYHFSGVHEGETVEILTPLGKGPVGRFLDIMEESRKESFDETDVAELLKVGPV
                     SRNLIGVPLIEDDRVTGLLAVVDRVGADEFGSGDVSFLRALANELSMTLHSYRLYGEV
                     TEEREKFHLLVDAVSDYAIYLLDTRGRVVSWNQGAARIFGYEADQIIGRHFSSFYSSS
                     DETWKEELSTVEGVGRLELEGMRLRNDGTRFMVNEVVSPVFDDENEMRGFAKVTRDIS
                     ELVKSHEEREELQSRLNQSQRLESVGQLAGGIAHDFNNLLSVILNCSHFLIDRLEEPD
                     PLRNDVLEIKAAAQRASSLTRQLLIFSRREMVQTQLVNLNDVIAELEKLLSRAIGEDI
                     AVRTTLFPELPSIEADPGQIEQVLMNLAVNARDAMPGGGMLLIETCPIRLDDAAASRF
                     VDLPAGNYVSLRVMDTGSGMPPEVVRKAFDPFFTTKPEGQGTGLGLATVYGIVKGVSG
                     HLTIDSVEGAGTTFEILFPVSETAVIRVEPQIAEIEVRGNGEVILLVEDERSLRAIGQ
                     RILTEHGFEVVEARNGREALFILEANGRNVDLLLSDIVMPEMSGVELFEQVQGRWPAL
                     PVALMSGYNPHLVAGELVDCEVLPKPFEPNDLLGFVKRMLERKPAGAN"
BASE COUNT          700 a         1069 c         1172 g          691 t
ORIGIN      
        1 taagcccgac gtggtcgcac cggggacgca cacgatctcg ttgcgttcac cgggtagcgc
       61 gatcgaccag gagtacggtt ccacggccgt tgttcaggac gactacttcc gcggcacggg
      121 aacttcgatg gccgcagcta cgacctcggg aatcgtcgcc cagatacttc agaaaacccc
      181 ttcgctcacc cccgaccagg tcaagaaccg attggtgtcg acggcccggc gcatcgcggt
      241 caccgacaag tacagcgttg gaaaaggact ggtcgacgcg tacgcggcag cgaagagcac
      301 gagcacggcg aaagcgaacc agggcttgct ctacggactc ggcggtctca gcaccggcct
      361 cggcctcatc gggcccgatc gcgggtctct gggtctcgga gtcgtgacac cgcagggaga
      421 aggggacctc gccggcgaga acaaggcaca acaccaggcg tcgctggttt cgctgagcaa
      481 cccgctggga ctgctgccgt ggacgagctt ggtctacacg accgttggat gggacccggt
      541 cacgtggctc actacctcct tcgccacgac cgattggtcg ggggtgcgtt ggaaagaaac
      601 ctcttgggac ggtgtccgct ggaaggagac ctcctgggac ggcgtccggt ggaagggcac
      661 cgcgtgggag aactccgact gggagggcgt tcgctggaag ggcaccgagt gggatggcgt
      721 gcgctggaag gccacctcgt ggcagacgcg ctggtacgcg gcggcgtgga aatagtcgat
      781 ccgagttaga cgagcagcga gccgaagacg gtgaccgaag aacgcgcact cccggctcgg
      841 ttctacgtct tcgtctcggt gctgtggtgc gggctcataa cagcgttcgt cctggcactc
      901 cgaaccgacc cagcgatggg gacacatctc tggacgatgg tggctttcat cgccctcttc
      961 gccaccgccg agctcctcga cctctcgtac cacgagaagg acgccacgtt ggggctgagt
     1021 caatgcgagt cgatcctgct cccaatgatc gttgctcttt cgttcgggga agtgctcgtt
     1081 gcggcggttg cggggatggg cttggcacgc gctttcgatc ggagcaccaa cctcggtaag
     1141 caggtgttca acctcgcgca gtacgggtgt gcagcggcgg cagcctcgtt cctgtatgtg
     1201 tggctgacgg ttgagagtgg tcgcttctcg atcgcagagg caggggccgc gacgatcgca
     1261 gtcatcgtct ttgaggtgct aacgcactcg ttcgtcgctg ccgccttaca cgttgccggc
     1321 cgaggtcgat tcatggacgc cttcacggac gtggcccccg tcaccttcat caatctcgca
     1381 ggcaatgtga tcctcggcct ccttttggct gctgcgttct cagccgaagc gtggacgatc
     1441 gcgctgttcc cgctcgcatc gatcgcgttg ttcctcggtt accgagcagt cgtgcgacag
     1501 caggctgagc gcaggaggat ggtgcgtctt cacgaggcaa gccgcgctct ggccgccgcc
     1561 cccgacctcg gccgggctct ggacggattc ctgaaagcgg ttgcgaaaac agcctcggct
     1621 ctcggggcgc gggccgtggt tcggatgaac gacggatacc acttctccgg cgtgcatgaa
     1681 ggggaaaccg tcgagatcct cactccgttg ggaaagggtc ccgtcggtcg tttcctcgac
     1741 atcatggagg agagtaggaa ggagtccttc gacgagaccg atgtggcgga gctacttaaa
     1801 gtcgggccgg tctcgagaaa cctgatcggc gtcccgttga tcgaagacga ccgcgtcacc
     1861 gggttgctgg cagtcgtcga tcgggtcggt gcagacgagt tcggctcagg cgatgtttcg
     1921 ttcctccgtg ctctggcgaa cgagctatcg atgaccttgc actcgtaccg gttgtacggg
     1981 gaagtaaccg aggaacggga gaagttccac ctcttggtcg acgcggtctc cgactacgcg
     2041 atctatctct tggacacacg cggccgggtc gtcagttgga accagggtgc ggccaggatc
     2101 ttcggctacg aggcggatca gatcatcggc cgccacttct cgtccttcta ttcctcttcg
     2161 gacgagacct ggaaggagga actgagcact gtcgagggcg tcgggcgtct cgaactcgag
     2221 gggatgcgtc tccgaaacga cggcaccaga ttcatggtga acgaagtggt ctcgccggtg
     2281 ttcgacgacg agaacgagat gcggggcttt gcgaaggtga cccgagacat cagcgagttg
     2341 gtcaaatctc atgaggagag ggaagaacta cagagtcgcc tcaatcaatc tcagaggttg
     2401 gagagtgtcg ggcagctcgc cggcggcatc gcgcacgatt tcaacaacct tctatccgtc
     2461 atcctcaact gttcccactt cctgatcgat cgtttggaag aacccgaccc gcttcgcaac
     2521 gatgtactcg agataaaggc ggctgcgcaa cgggcgagct cgctcacgcg tcaacttctg
     2581 atcttcagcc ggcgtgaaat ggtccaaacc cagctggtca acttgaacga tgtcatcgcc
     2641 gagctcgaga agcttctcag ccgcgctatc ggggaggaca tcgccgtccg aacaactctc
     2701 tttccagagc tgccttctat agaggccgat cccggacaga tcgaacaggt gctcatgaac
     2761 cttgccgtca atgcgcgcga tgcgatgccg ggtgggggga tgctgctgat cgagacctgc
     2821 cccatacgcc tggacgatgc cgcggcatct aggttcgtgg atctcccggc cggcaattac
     2881 gtgtcgttgc gagtgatgga caccggcagt ggaatgccgc cggaggtcgt tcgaaaggcg
     2941 ttcgatccct tcttcacgac gaaaccagag gggcagggaa cgggtctcgg gcttgcgacc
     3001 gtgtacggga tcgtcaaagg tgtctcgggc cacttaacga tcgactccgt cgagggggca
     3061 ggaaccacct tcgagattct ctttccggtc agcgagaccg cagtcatccg ggtggaaccg
     3121 caaatcgccg agatcgaggt acggggcaac ggcgaggtga tcctactggt ggaggacgag
     3181 agatcgctgc gcgccatcgg ccagaggatc ctgaccgaac acggcttcga ggtcgtcgag
     3241 gcgcgcaacg gccgcgaggc gttgttcatc ctggaggcaa acggccgcaa cgtcgatctt
     3301 ttgctctcgg acatagtcat gccggagatg tccggggtgg aactcttcga gcaggtgcaa
     3361 ggtcgatggc cggcccttcc ggtcgcgctg atgtccggtt acaacccgca cctcgtcgcc
     3421 ggcgagctgg tcgattgtga agtcctgccg aaacccttcg aacccaacga cctactcgga
     3481 ttcgtgaagc gaatgctcga acgcaaaccg gcaggagcca attgatggct tctccagccc
     3541 ccagccaagg aggaagcatg gccctgaccg aaacacgtac agagacgcga tcggaggatg
     3601 tctttagccg gatcaaggaa acggcgcgca ta
//
LOCUS       DATMED010000335         8126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5647799, whole genome shotgun sequence.
ACCESSION   DATMED010000335 DATMED010000000
VERSION     DATMED010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5647799"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VNP73_08640"
     CDS             <1..701
                     /locus_tag="VNP73_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66026.1"
                     /translation="ATSDALLMNPAIDGSLVAWESIGGNANVKARYLGRRVQSLGGGP
                     GDQIMPAVSGNWVAWWDLRRIPKIVARNMKTGARRVVTPSDDFTLMGPPGLGGRFVYW
                     WQDSDFDGIGSIVRMDLKSKKRRTVVPQSSDFAPVWGLSITFPPVPSANDRFLVYTSE
                     IAYLIEFAFDPDLIPNDQVGRDVFIVPISGGQVRPVTDNRADQAHPVIADGRRVLWLD
                     SSQATTDLMTREVP"
     gene            839..1714
                     /locus_tag="VNP73_08645"
     CDS             839..1714
                     /locus_tag="VNP73_08645"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HWL66027.1"
                     /translation="MVSQLPRAPFDCVLVGAGRVGTAVALLLQSKGHNVVAVSSRSRD
                     SAAAAAERLGAPVTSPDDLPAADLYLIGTPAASLPEIGRSLVATNRVSGSVLVHFAGA
                     VGIEPLRECSDRGAFVGALHPVQACPDVDSARRNLPHSAWGITCTDGLDVWAKRLVEE
                     DLEGRAVAVAEKDRPLWHAGAVITSNGIAALLAAGEALLGDLGVEAPEAVLGPLAAGT
                     IANARSGGGGAATLTGPLVRGEVDTIADHLGQLEALDPNHSRNYRLASQLILSAIRAR
                     GGGATGAAIEALVQP"
     gene            1711..2553
                     /gene="panC"
                     /locus_tag="VNP73_08650"
     CDS             1711..2553
                     /gene="panC"
                     /locus_tag="VNP73_08650"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWL66028.1"
                     /translation="MKIVRTAGELRAELAEERAADRTVGFVPTMGALHEGHLSLVRLA
                     RSSTAAVVVSIFVNPLQFGPSEDFEAYPRDEAHDLELLEREGVDIVFIPRARDVWAQP
                     PTTLVSVGELSRIVEGAIRPGHFDGVATVVATLFNLVRPDVAYFGQKDAQQVAVIKKM
                     VTDLAWPIEIRVGPTVRDPDGLALSSRNVYLNAAERTRATSLWRALQEGERSLASGDA
                     LETTEKVMWEALVAGGVEPDYASVVDPDTFGPVDTQAGSLLVIAAHVGSTRLIDNLLV
                     NREA"
     gene            2553..2921
                     /gene="panD"
                     /locus_tag="VNP73_08655"
     CDS             2553..2921
                     /gene="panD"
                     /locus_tag="VNP73_08655"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWL66029.1"
                     /translation="MQRTMLKSKIHRATVTDANLDYVGSITIDPSLMRAADLRPHEQV
                     HVLDISNGERFITYVIEGQEGSGEMCVNGAAARLVQIGDKIIVVSYAAYDEKELASYA
                     PTVVAVDANNRRAALDVARV"
     gene            2922..3701
                     /locus_tag="VNP73_08660"
     CDS             2922..3701
                     /locus_tag="VNP73_08660"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWL66030.1"
                     /translation="MLLAIDVGNTQTHLGVYEKDELVHQWRASTDARRSADELALMFG
                     EFLGLADLSFSRQITGVCIASVVPRATQQLREMTLRYFGYPALVVEPGVKTGLAVLTD
                     NPKEVGADRICNAVAARELYPDNAVVIVDFGTAIKVEAINAAGQFLGGAIAPGMEIAA
                     TALFSAAARLTRVEFVAPPSAIGRSTVTALQSGIIFGTAALVDGLVGRVTEELEGEVK
                     VIATGGLAPAVMSHCETIEELEPTLTLMGLRVIFDRNADDA"
     gene            3698..5116
                     /gene="lysS"
                     /locus_tag="VNP73_08665"
                     /pseudo
     CDS             3698..5116
                     /gene="lysS"
                     /locus_tag="VNP73_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
     gene            5163..6584
                     /locus_tag="VNP73_08670"
     CDS             5163..6584
                     /locus_tag="VNP73_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL66031.1"
                     /translation="MKSFPAAVLACLLFISCQPARDENDFTLPVPNPTPSSSNTHIIG
                     LVGTLSGPDSWRGDDAFKGADLAVHELNRELEANETQFELVTLDDRGDADRATDLVKE
                     LASSDRAVGIVYAGPPEGLPPAERSLSRMGVPAIICHGDLYGARLLSPHVFQTSPSFL
                     WQSRRIVDYLLGDRGYRRIGLASEISLTGRTARKNLAVALEEEGAGLAGSVTYRPEDL
                     DGARIVDALRRRRVQAVVIQASPPSALKIQRSLRLNGARYRNTRAARASSRATKAWVP
                     QVVGFDQTMSAALEPVDLPAGTIASDSYDRGVHYLPIPNFARFRQAFRNWWDREPLGW
                     ERRSYDAVHMIGWAIDEAGIKEARPDRDLAPVLEKMDGTRFGGLGITLGPDDHTTVEQ
                     STVGLWVVPRLGISVPELGLVPNSMPWVPLARGFSIDGERTDIQPQDWKWLFENAPPK
                     KAPAPRLSRARFGVTTSSRDPIH"
     gene            6798..>8126
                     /locus_tag="VNP73_08675"
     CDS             6798..>8126
                     /locus_tag="VNP73_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWL66032.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEKVRQQVEEIIGAGQSPPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLGRVRQQVIQLLSGYSGAKEGAGAAGGEAP
                     QGSLVLDQFGRNLTQLARENKLDPVIGRDKEIERVMQVLSRRTKNNPVLIGEPGVGKT
                     AIVEGLSQKIVKGDVPETITGKQLYTLDLGALVAGSRYRGDFEERLKKVLKEIKTRGD
                     IILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHLEKDAALE
                     RRFQPIKVPEPTVAHTIDILKGLKDRYEAHHRVHITDQGIVAAANLADRYISDRFLPD
                     KAIDLIDEAGSRLRIRRMSAPPDYRELEERIADVRRQKEDSIEAQDFERAA"
BASE COUNT         1509 a         2460 c         2643 g         1514 t
ORIGIN      
        1 ttgcgacgtc ggatgcactc ttgatgaacc cggcgatcga tgggtccctg gttgcgtggg
       61 aatccatcgg tgggaacgcg aacgtcaaag ctcgatacct gggacgtcgg gtccagtcgt
      121 tgggcggcgg ccccggtgat cagatcatgc cggcggtgtc ggggaactgg gttgcctggt
      181 gggacctgcg tcggatcccc aagatcgtcg cccgcaatat gaagaccggc gcgcgccgcg
      241 tcgtgacccc gtcggacgac ttcaccctca tgggtccgcc gggcttggga ggtcggttcg
      301 tgtactggtg gcaggactcg gatttcgacg ggatcggatc gatcgttcgc atggatctca
      361 agtcgaagaa gagacgtacc gtcgttcccc agagttcgga cttcgccccg gtgtggggtt
      421 tgtcgatcac ttttccgccg gtgccatctg cgaacgatcg cttcctcgtc tatacgagtg
      481 agatcgcgta cctcatcgag ttcgctttcg atcccgatct gatccccaac gaccaggtgg
      541 gcagggatgt cttcatcgtg ccgatctcgg gaggacaagt gcggccggtt accgacaacc
      601 gggccgatca agcgcacccc gtgatagccg acggtagacg ggtgctctgg ctcgactcgt
      661 cgcaggcgac caccgatctc atgacgcggg aagtgcctta ggccatttgt cctccctcag
      721 atatgcccgc tgcgcgggca gctcttcgct tcggacgagc gatcgtcgag acgatcgctc
      781 cttcgccggg atatatcctg tcgcaccgac cggtacccaa cgtggactgg agcgattgat
      841 ggtgtctcaa cttccccgcg ccccgttcga ttgtgtgttg gtcggcgccg gccgcgtggg
      901 taccgcggta gccctcctcc ttcagagcaa aggccacaac gttgtggccg tctcgtcccg
      961 ctcgagagat tcagcggcgg ctgccgccga gcggctggga gcgccggtaa cgtcaccgga
     1021 cgatctcccc gctgccgatc tgtatctgat cggaacaccc gcggcctcac ttccagagat
     1081 cggtcggagc ttggttgcaa cgaatcgcgt ctccggctcc gtcctggttc atttcgccgg
     1141 agcggtgggg atcgagccgc tgcgtgagtg ctccgatcgg ggagccttcg tgggcgcgct
     1201 ccatccggtg caggcatgcc ccgacgtgga cagcgcgcgg cgcaatctcc cacactcggc
     1261 gtgggggatc acctgtaccg acggtctgga cgtgtgggcg aagcgcctgg tagaggaaga
     1321 tctcgaaggt agggccgtcg ccgttgccga gaaggatcgc cccctgtggc acgcgggggc
     1381 ggtcatcacc tccaacggga tcgccgctct tctcgcagcg ggggaagccc tgctcggaga
     1441 tctcggggta gaagcgccgg aggccgtcct cggccccctt gccgccggca ccattgccaa
     1501 cgctcgctcc ggtgggggcg gagcggctac cttgaccggc cccctcgtgc ggggtgaggt
     1561 cgacacgatc gccgaccatc tgggccaact cgaggctctc gatcccaacc attcccgcaa
     1621 ctaccggctt gcgtcccagc tgatcctgtc tgcgatccgt gcgcggggtg gcggcgcgac
     1681 cggcgcggcg atcgaggcgt tggtgcaacc gtgaagatcg tccggaccgc gggcgaactg
     1741 cgcgccgagc tcgccgaaga aagagcggcc gatcgaacgg tgggtttcgt tccgacgatg
     1801 ggggcgctgc acgaggggca tctgtcgctc gtgagattgg ctcgttcttc gaccgctgcg
     1861 gtggtcgtca gcatctttgt caatcctttg cagttcgggc cgagtgaaga cttcgaggcc
     1921 tatccgcgcg atgaagccca tgacctggag cttttggaac gggagggcgt ggacatcgtt
     1981 ttcatccctc gtgctcggga tgtgtgggcc cagccgccga cgacactcgt gtcggtcgga
     2041 gagctcagcc ggatcgtcga gggggcgatc cggccgggtc acttcgacgg tgtcgcaacc
     2101 gtcgtagcca cgctcttcaa tctggtgagg ccggacgtcg cctacttcgg gcagaaggat
     2161 gcccagcagg tcgccgtcat caagaagatg gtgaccgatc tcgcgtggcc gatcgagatc
     2221 agggtaggtc cgaccgttcg ggatccggat gggctcgcgt tgagcagccg caacgtctac
     2281 ctgaacgcgg ccgagcgaac ccgagcgacg agtctgtggc gggcactcca ggaaggcgag
     2341 agatcgctcg cctccggcga tgccctggag acgacggaga aggtcatgtg ggaagcgctc
     2401 gtcgccggag gtgtcgagcc cgactacgca tccgtggtcg atcccgacac gttcggccct
     2461 gtggatacac aggcggggtc tcttctcgtg atcgcggccc acgtcggctc gacacgtttg
     2521 atcgacaacc tgctcgtaaa ccgggaggcg tgatgcaaag gaccatgctg aagtcgaaga
     2581 tccatcgtgc gaccgttacc gacgcgaatc tggattacgt aggaagcatc accatcgatc
     2641 cgtccttgat gcgcgcggca gaccttcggc cccacgagca ggtgcacgtg ctcgatatca
     2701 gcaacggaga gcgcttcatc acctacgtga tcgagggcca ggaggggtcg ggtgagatgt
     2761 gcgtcaacgg agctgcggct cgcttggtcc agatagggga caagatcatc gtggtttcat
     2821 acgccgctta cgacgagaag gagcttgctt cctacgctcc cactgtcgtg gcggtcgacg
     2881 cgaacaaccg gcgcgctgcg ctcgacgtgg ctcgggtcta agtgcttctg gcgatcgacg
     2941 taggtaacac ccagacgcat ctgggtgtgt acgagaaaga cgagctcgtc catcaatgga
     3001 gagcatcgac cgatgcccgc aggagcgcgg acgaactcgc cttgatgttc ggggagttcc
     3061 tcgggctggc ggatctgtcg ttctcgcgcc agataacggg tgtgtgtatc gcctcggtgg
     3121 tgcctagggc tacgcaacag ctccgcgaga tgactcttcg ctacttcggg tacccggcgc
     3181 tggttgtgga acccggggtc aagacggggc tcgccgtgct caccgacaat cccaaggaag
     3241 tgggagcgga tcggatctgc aatgccgtcg cggctcggga gctctatccc gacaacgcgg
     3301 tggtcatagt ggacttcggg accgccatca aggtcgaggc gatcaacgcg gccggccagt
     3361 tcctcggagg tgcgatcgct cccggcatgg agatagcggc caccgcactc ttttccgcgg
     3421 cggcacggct cacacgggtc gagttcgtgg ccccgccgtc ggctataggc cgaagcaccg
     3481 tcacggctct acagtccggg attatcttcg ggacggctgc cctcgtggat gggctggtcg
     3541 gaagagtgac cgaggaacta gagggagagg tgaaggtgat cgcaaccggt ggactggccc
     3601 ctgcggtcat gtcgcattgc gagacgatcg aggagctcga gccgaccctg accctgatgg
     3661 gtctacgcgt catcttcgac cgcaacgcgg acgacgcgtg agcgactatc cctaccggta
     3721 cgagcgaacc cttcgggtcg ccgacctccg tgaacgtttc gacgaacttg agcccggtgc
     3781 cgagagcggc agcgaggttc gcgtcgcggg acgactcctg agtatccgca agcaaggaaa
     3841 ggcggcgttc gccgaccttc gtgacgggat ggaccgcatc cagttgttcg cgaaccagaa
     3901 ggtactggga gacgacggga tggactcgtt cgtctccttg ggcgtcgggg acatcgtggg
     3961 ggccgcggga gaggtgatca agacccgtcg gggggagctc tcggtcaagg tcgatgaagt
     4021 gatcctgctg gcggagtgca tcaggcctat gccggagaaa tggcacggcc tctccgacat
     4081 agaggttcgg tatcgccagc gatacctcga ccttctcttg aatgaagaac cgcgccgtgc
     4141 cttgcacgcg cgcgctcgtg cgaacagcgc gatcagggcc ttcttcgatg ggaggggatt
     4201 cagcgaggtc gagacaccca tccttcaccc gatcgccggt ggtgccgtcg cacgcccgtt
     4261 catcacgcac cacaacgcgc tcgatatcga cctgtacctg cggatagccc ccgagctcta
     4321 tctgaagcgc ttgttgatcg ggggcttcga tcgcatctac gagttgaatc gaagtttccg
     4381 caacgaaggc atctctcccc ggcacaaccc ggagtacacg atgctcgagg cgtacgaggc
     4441 ctacgtggat ttcgaagaca cgatgaatct ggtcgaggat ctggtcaaag agatagctca
     4501 agccgtgaac ggatcactcc gcttcgaggt cgatggtcac gagttggatt tctcggcgcc
     4561 tttccaacgc cggacgatgt tcgaggtcat ccagcaagcg acgggcaagg accttctgcc
     4621 cgtctggaca gcgggcgacg ccggttccct gggagcgatc ggagacgagt tgggagccgc
     4681 cattgacccc aagtgggggc cgggcaagat cctgctcgag atcttcgaag cgacgacgga
     4741 gaaggctctg atcgacccga tcttcgtcgc gggattcccg aaagaggtat cgccacttgc
     4801 gaaagaccat cgcagcatcc cggggttcac cgaacatgcc gacctcatca tcggaggagt
     4861 ggagatagcg ccttgctact gagagctgaa cgaccccgag gaacaaagac gtcgcttcga
     4921 acaacaggcc gcggctcgcg ccgccggcga cgacgaggct tcgcttgttg atgaagactt
     4981 cttggaagct ctctccttcg ggatgccccc ggccggcgga tttgggctcg gcatcgaccg
     5041 cttgctgatg ttgttgctcg gtctgccttc acttcgagaa gtcatcctct tccccacgct
     5101 caagcccggt cagtaggcgg ggagatagtc accctgccgt tcagtggcag gattttgccc
     5161 ccatgaagtc tttccccgca gccgttctcg cctgcctgtt gttcatctcg tgccaaccgg
     5221 cacgcgacga gaacgacttc accttgccgg ttccgaaccc gacgcccagc agcagcaaca
     5281 ctcacatcat cggactggtc ggaaccttga gtggtcccga ctcgtggaga ggtgacgacg
     5341 cattcaaggg ggccgacctg gcggtgcacg aattgaatcg ggaactagag gcaaacgaaa
     5401 cccagtttga actcgtgacg ctcgacgacc ggggcgatgc ggatcgcgcc acggatctgg
     5461 ttaaagaact agcgtcttcc gatcgggcgg tgggcatcgt ctacgccggc cctcccgagg
     5521 gattgccgcc ggcggagcga tctttgagtc ggatgggcgt cccggcgatc atctgtcacg
     5581 gcgaccttta tggggcgcgg cttctctccc cgcacgtatt tcagacgtcg ccttcgttcc
     5641 tctggcagtc gcgtcgcatc gtcgactacc tactggggga tcggggctat cgccggatcg
     5701 gtctggcgtc tgagatctcc ctcaccggca ggaccgcacg caagaacctc gccgttgcac
     5761 tcgaagaaga gggagcggga ttggccggct cggtcaccta ccgtccggag gacctcgacg
     5821 gagcccggat cgtcgacgcg ctcaggcgca ggagggttca agcggtcgtt attcaagctt
     5881 cgccaccctc cgctctgaag atccaacgat cgttgcgact taatggtgcg aggtatcgga
     5941 acactcgggc cgcgcgagct tcgtctcgag cgacaaaagc ctgggttcca caagtcgtcg
     6001 gcttcgacca gacgatgagt gcggcgttgg agccggtcga tcttcctgcg gggaccatcg
     6061 cgtcggattc ctacgaccgt ggcgttcact atctgccgat cccgaatttc gcgaggttcc
     6121 gacaggcgtt ccggaattgg tgggatcgag aaccgttggg ctgggagcgc cggtcctacg
     6181 atgcggttca catgatcggc tgggcgatcg acgaagccgg cataaaggag gctcgtccgg
     6241 atcgcgacct ggctcccgtg ctcgagaaga tggacggcac tcgattcggt ggattgggga
     6301 tcaccctggg cccggacgac cacacgaccg tcgagcagtc gacggtgggt ctctgggtgg
     6361 ttcctcgttt gggtatctct gttcccgaat tgggcctcgt tccgaactcg atgccctggg
     6421 tccctctggc ccgcggcttc tcgatagacg gggagagaac ggacattcag ccccaggact
     6481 ggaagtggct gttcgagaac gcgcctccca agaaggcccc ggcccccagg ttgtcccggg
     6541 cccgtttcgg ggtaaccacg tcgtctcgtg acccgatcca ctgacagcag agagtgaccg
     6601 acgaacctga taggaccgct aaggaaggaa cggtccccgg ccgattaatt atccggggaa
     6661 cgttttgtag gggttcatcg ttctaaagga tgaggtggcg attccagagg tgggaaagac
     6721 cacttcaata taatcagcga ccaatccgga tagagcttcc ggctcgtatc cggtggagat
     6781 gaaagggagg gccccacttg tttgaacggt ttaccgaccg ggcccgaagg gttgtagtcc
     6841 tcgcgcagga ggaagcccgg cttctcaatc acaactacat cgggaccgag cacatcctgc
     6901 tcggcttgat ccacgaaggc gaaggcgtcg cggcgaaagc cctcgaatcg ttggggatct
     6961 cgctcgagaa ggtgcgtcaa caggtcgagg agatcatcgg agcaggtcag tcgcctccgt
     7021 ccggccacat ccccttcacg ccacgggcga agaaggtcct cgagctatcg ctcagagaag
     7081 cgctccaact cggacacaac tacatcggca ccgagcacat cctgctcggc ttgatccgcg
     7141 agggcgaagg cgttgccgca caggttctcg tgaagctcgg cgccgacctt ggtcgggttc
     7201 gccagcaagt catccagctg ctctccggct actcgggtgc caaagaaggt gcgggcgctg
     7261 ccggcggcga ggctccgcaa ggttcgctcg tgctggacca gttcggccgg aacctgacgc
     7321 agctcgctcg agagaacaag ctggaccccg tcatcggacg cgacaaagag atcgagcgtg
     7381 tgatgcaggt tctctcacgc cgtacgaaga acaatccggt tctgatcggc gagcccggtg
     7441 ttgggaagac ggcgatcgtc gagggcttgt cgcagaagat cgtcaagggt gacgtccccg
     7501 agacgatcac gggcaagcag ctctacacgc tcgacctcgg tgcactggtg gccgggtccc
     7561 gctatcgcgg tgatttcgaa gagcgtctca agaaggttct taaagagatc aaaacccgcg
     7621 gcgacatcat cctgttcatc gacgagctgc acactctcgt cggtgcgggg gccgcagaag
     7681 gtgcaatcga tgccgcaagc atccttaagc cgatgctggc gcgcggcgag ctccaaacga
     7741 tcggtgcgac caccctcgac gaataccgga aacatctcga gaaggacgca gcgctcgaac
     7801 ggcggttcca gccgatcaag gtgcccgagc cgacggtcgc gcacacgatc gacatcctca
     7861 aggggttgaa ggaccgttac gaagcgcatc accgggttca catcactgac caggggatcg
     7921 tcgcagcggc aaacctggcc gacaggtaca tcagcgaccg cttcctgccg gacaaggcga
     7981 tcgacctgat cgacgaagcc gggtcgcgcc tgcgcatccg tcgcatgagt gcgccgcccg
     8041 actaccgcga gctcgaagag aggatcgccg acgtccgacg tcagaaggaa gactccatag
     8101 aggcacagga ctttgagcgc gcggcg
//
LOCUS       DATMED010000336        12208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5271889, whole genome shotgun sequence.
ACCESSION   DATMED010000336 DATMED010000000
VERSION     DATMED010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5271889"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VNP73_08680"
     CDS             <1..262
                     /locus_tag="VNP73_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWL66033.1"
                     /translation="PPTHMVSVYGADKPGIVYRVAGSLAERGVNITDLTSRVIGPEGA
                     PVYALMLEVVATDETRLRGDLRAVSEELGIDVSVRALEVDVL"
     gene            265..762
                     /locus_tag="VNP73_08685"
     CDS             265..762
                     /locus_tag="VNP73_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWL66034.1"
                     /translation="MAVRPVVRLPDRVLKRRAGDLGGVSDEELAVDLVDTMRASPACV
                     GLAAPQIGVPKRMFALDVSEHPKTTTCHGLVVMWDPLIVSSEGREVGREGCMSVPDLT
                     ANVGRAVRIVVSGMTPEGGPRVLETEGFEARAFQHEIDHLDGLLILDRVASLHTDVFR
                     RRTYR"
     gene            complement(767..2365)
                     /locus_tag="VNP73_08690"
     CDS             complement(767..2365)
                     /locus_tag="VNP73_08690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWL66035.1"
                     /translation="MSTTRSQNHPRSVEIASLGERMGYLQALRAALAAIVLASTVLVP
                     DVVGAGLDDVMFVTAGYLLLSAAVEGVRSAGSGRGLTAVGGMLIMDGLFLAWVTYQTG
                     GTQSPLRFLVYLHLIAVTLLASHRTGLKIALWHSLLFFVVFYAQAARIIAPLEAATET
                     GGGFQKPSFFNVVAFWLVALVTTAFSSINERQLRRNQSDLESLARTAEKLDDSQSPRD
                     VAQALLDGLIDTFEFDRGTVLATEGALDPALLAYKGPGEPAEVKPHLDEAIARAWSER
                     KPQLLKKLNAESDPQLTALLPFAKSLVVAPMFADGSPIGVVALEYASRKQHRIERRTI
                     DMVSQFAAHGALALRNAWLVEEIRRMADTDGLTGIPNRRTFQTMLEKELSRAQRMGDQ
                     VTLVMLDVDKFKDLNDKHGHQVGDEVLKHVAASLLENCRDFDTAARYGGEEFAAILPG
                     CSIQESLVAAERLRKGIAAPEGPVPVTASAGVATFPTHAGNVEGLIKAADEALYESKR
                     SGRDRVTRSRRKAPAAKPVAEDVP"
     gene            complement(2366..3406)
                     /locus_tag="VNP73_08695"
     CDS             complement(2366..3406)
                     /locus_tag="VNP73_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66036.1"
                     /translation="MEESRPKSYARRVVLAMVLLCLLIPAGAMADPLGDVGDDASDAV
                     GDATDSVDDATDHVSDTLSDAGDQASDAVDDATDAAGDAVSDVGDEVDDATGGATDDV
                     TDVVDDVIDDVEEEVDETVDEVTDEVGETIDDTQDAVDETVDEVVEDANDTVDGVIGG
                     GGGGTGGSDPATNPTDQVPGDPDAAPRVGKPSGARSVSGEGSGSVTSGASPASSLPYL
                     MRSYPSSRGATQVGLGGHEGAASRRPSATRTERSNSAGAGSREAAAPQVNPAWPGRFV
                     TSERYPDNAVDSFLHDLADQAAETARRVAFPVALLGIVLAFLAVHGRVDRRDPKLSLA
                     PVDPEEEVLYFE"
     gene            3641..3919
                     /locus_tag="VNP73_08700"
     CDS             3641..3919
                     /locus_tag="VNP73_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWL66037.1"
                     /translation="MNKKELIDSIADKTGESKRTVNEVLDTTITQIQSQVKKGERVSL
                     PGFGTFERRNRSARTARNPRTGEEIKVPATKVPAFKPGSSFKDFVAGR"
     gene            4064..4444
                     /locus_tag="VNP73_08705"
     CDS             4064..4444
                     /locus_tag="VNP73_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66038.1"
                     /translation="MKRAALGGAHRGPVDASRQRRTIRLVQILLVLISAGLLMFAGYS
                     FGRVSGYRSARSGAVFERPRPPSPAQPIVLTILGGIAIAGAAALQQGSSVRMPTPARL
                     DELAGRAEQTALERAEKAEEAEKA"
     gene            complement(4454..5107)
                     /gene="cofC"
                     /locus_tag="VNP73_08710"
     CDS             complement(4454..5107)
                     /gene="cofC"
                     /locus_tag="VNP73_08710"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HWL66039.1"
                     /translation="MDAGILPVKNLSRAKHRLAEHLDDADRLEVARALLEDSLALCRD
                     ASFLTWWVASDDEEALTRAHDYGFNTVKDQTGTLNGALQQAVLVAMAEGATSVTMIPS
                     DIPLAYSGDLRDLLDTGSTSEVVVVPSARDGGTNGLYLRPPDILAPRFGTASMQAHLM
                     LAERLGFRCSILVLPRLALDIDTIEDVDEFLTRERTGESKSAEILAKLRPHVNGAES"
     gene            5173..6330
                     /locus_tag="VNP73_08715"
     CDS             5173..6330
                     /locus_tag="VNP73_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HWL66040.1"
                     /translation="MDKSARKHWKHLYGEVVATEICCGCAACVVACPHKVLEMQDFDP
                     IQTDILSPFDNCVHGEEGCSLCAMACLRLEPALDAIETLVFGARRKSDQPEGAYRSKT
                     LARATDERIISRGQDGGAVTALLAWGLDQGELDGAVVASPSATVPWLDEPLLVRSSEE
                     LLGAAGSRYTYCSTPLGLRQAAAAKCKNVALVGVSCESTAVRELTAEGIKRWTRPVKL
                     VIGLMCCETFDYEAFMIGKVQQELGISLDDIVKVNVKGKVIVTLKDGRDIDIPLKDAR
                     PFANEWCHHCPDFAAEHADLSCGGLGMEGWTMILVRSQTGQDYLERAVEAGVLELRPA
                     EEEPKALEVMDRLARKQRERIDPWDPHAATRWPTEQALNAARAEAAAEGAG"
     gene            complement(6332..6634)
                     /locus_tag="VNP73_08720"
     CDS             complement(6332..6634)
                     /locus_tag="VNP73_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWL66041.1"
                     /translation="MIVRIFDTAMDPADVEKGKELFRQKVRPAFDSFAGCHGVEMVIG
                     VEEHSQDLVDIASISRWDSIESIEQATTSDDYKEALEEIRKLFQQAPIVRHFELTE"
     gene            complement(6705..7541)
                     /locus_tag="VNP73_08725"
     CDS             complement(6705..7541)
                     /locus_tag="VNP73_08725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWL66042.1"
                     /translation="MEGDRLTIAQFSDIHCGHPMFDAELMEHVVDEIVDLDPDLVVCA
                     GDLTSEGYAPQFRQAKRYLDRLGDLEMVVIPGNHDLMNVGFLHFRDQFGKSDRVVRVP
                     FCPPGGGQPQRYATLVAVNSSKPDLADGEVGHIRYDWIRACYEWPDDYRIFVLHHHLV
                     SVPGTGRERNIVWDAGDLLALLTELKVDLVMSGHKHVPNVWQFGRMLLINSGTASSHR
                     VRGYTRPSYNVIEVGEDRVRVVQRYPGLGEIVAANYTFKERELKLNPQLAGMFNRGAW
                     NV"
     gene            7662..8345
                     /locus_tag="VNP73_08730"
     CDS             7662..8345
                     /locus_tag="VNP73_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWL66043.1"
                     /translation="MPMEPTYALARAVLKPWLGTWFRWHLEGMENIPRSGPALLAINH
                     IAYLDPLAAAYVVDKAKRIPRFLAKSELFDDKRIAWVLRGAGQIPVKRGSSDAPMALD
                     HAIEALREGEIVVIFPEGTITRDPDLNPMEGKTGIARLALTTKAPVIPCALWGTANIW
                     PKGYGKRWRPRQDILVRVGEPMHLLGDPESRDDWSEAVQRVMDRISLLLASLRPAIPD
                     RRRPRRSAA"
     gene            8342..9394
                     /locus_tag="VNP73_08735"
     CDS             8342..9394
                     /locus_tag="VNP73_08735"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWL66044.1"
                     /translation="MSKVAFVGAGSWGTATAALVARKNNAEAVLWARRGELAETINLF
                     HENPEYLSGIKLPENLRATNDLEDALEGADTVVMGVPSHGFREVLREVAPVAGRDVCY
                     VSLTKGLEIDTRSRMSQVLAEEVDGIDQSNIAVLTGPNLAREVVQDFPAASTVACNDD
                     ATAARLQEIFHTPTFRAYTNHDVVGCELGGAFKNVIAIAAGIADGLGFGDNTKATVMT
                     RGLAEMARFSVKFGAQPITFLGLAGVGDLIATCASPQSRNHSVGIELGKGRKIDDIIE
                     SMNMVAEGVKSCKPIFELGQEADVWMPLTENVKRICHDGVSVEEVVSDLLAREIRSEF
                     AGIEEYLTFNEQDVRS"
     gene            complement(9398..10588)
                     /locus_tag="VNP73_08740"
     CDS             complement(9398..10588)
                     /locus_tag="VNP73_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWL66045.1"
                     /translation="MSRKRPHGIGTRAVHPPRPPRQEGEPVAPVLDLASTYAFDSTES
                     FAKASEEKVGAGYVYTRWANPTVDAFEAAVADLEGAQDAEAFASGMAAISTVLLAECS
                     AGDRIVAARQLYGGTFELLSQTLPRYGIQTTFCDVADHASIEDALDGAKLLYCETIGN
                     PRVQVADIPALAKLARAAGVPLLVDNTFASPVLCRPLEHGATASIHSATKFLGGHNDL
                     IGGVVCAEPGVIERMRHIAREFGAVLSPFNAWLALRGIATIHLRVLRASESALLVAQA
                     LAEHPGVSAVFYPMLDGDPGRALAERLLGGYGGGMLAFDVAGGKEQAGRFQEELKVVQ
                     RAASLGGTRSLIVHAASVTHTQLDAEQLAAAGISEGFCRLSVGIEDPEDLITDLVEAL
                     DRAG"
     gene            complement(10598..10837)
                     /locus_tag="VNP73_08745"
     CDS             complement(10598..10837)
                     /locus_tag="VNP73_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWL66046.1"
                     /translation="MADVQAYILIQTEVGKAAQVAKEVRSIDGVDAAEDVTGPYDVIV
                     RASASNVDDLGKLVVAKIQAVEGITRTLTCPVVHL"
     gene            11017..>12208
                     /locus_tag="VNP73_08750"
     CDS             11017..>12208
                     /locus_tag="VNP73_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWL66047.1"
                     /translation="MTDPGKVFVNLSPPHLVEHSLERNEGILERSGALVTRTGTRTGR
                     SPGDRFFVSHGPSKEQIDWGPVNKPFEPSSFEALFDRVRRHLRGRELYVVDGLLGADP
                     THQIKLRVVCERPWHALFGRQLFRRPSEEQLRTFEPEFTLLAAPSFEAVPDRDGTNSE
                     AFIGIDLESKQVLICGTEYAGEMKKSMFTSANYLFPQQGVLPMHCSANVDEEGDVALF
                     FGLSGTGKTTLSADAHRRLIGDDEHGWSDSGIFNFEGGCYAKCINLSREREPQIWAAI
                     KFGSVVENVVVDEATRIVDYDDDAITENTRAAYPLDFIPGFVPEGRAGHANTVVFLTA
                     DAFGVLPPISRLGPEGAMYHFLSGFTSKLAGTEAGVGSEPQATFSTCFGAPFLPLKPS
                     VYAEM"
BASE COUNT         2183 a         3793 c         3991 g         2241 t
ORIGIN      
        1 gccaccgacc cacatggtgt ccgtgtacgg cgccgataag cccggcatcg tctaccgggt
       61 ggccggatcg ctcgccgagc ggggcgtgaa catcacggat ctgacgtcca gggtcatcgg
      121 tcccgagggc gccccggtct atgcgctgat gctcgaggtc gtcgcgacgg acgagacgag
      181 attgcggggt gatctcagag cggtcagtga agagctcggg atcgatgtga gcgttcgcgc
      241 tctagaagtc gacgttctct aagagtggcg gttcgccccg tcgtccgcct tccagatcgc
      301 gtgctcaaac gacgcgcagg cgatctgggt ggtgtctcgg atgaagagct cgccgtagat
      361 ctcgtcgaca cgatgcgcgc ctcgcccgcc tgtgtgggcc ttgccgcgcc ccagatcggg
      421 gtccccaagc gcatgttcgc gctcgacgtc tcggagcatc ccaagacgac aacctgtcac
      481 gggttggtag tcatgtggga tccgctcatc gtctcttccg aggggagaga ggtaggccgc
      541 gagggatgca tgagcgttcc cgatctgacc gcgaacgtcg ggcgcgccgt gcggatcgtc
      601 gtgagcggga tgactccgga gggcgggccg agggtgctgg agacggaagg gttcgaagct
      661 cgagccttcc agcacgagat cgatcatctg gatgggcttc tgatcctcga tcgcgtcgcg
      721 tctctccaca cggacgtctt tcgcaggagg acgtaccgct agcgtcttaa ggaacgtcct
      781 cggctaccgg tttcgcggcg ggggccttcc tgcgtgatcg cgtgaccctg tctcgccctg
      841 aacgcttcga ttcgtacaga gcctcgtctg ccgccttgat caacccttcg acgttcccgg
      901 cgtgtgtcgg gaatgtggca acgcccgcgc tcgcggtcac ggggaccggt ccttcgggag
      961 ccgcgatgcc tttgcggagc ctctcggcgg ctacgagtga ctcctgtatc gagcaccccg
     1021 gcaggatcgc agcgaactcc tcaccgccgt agcgcgcggc cgtatcgaaa tcgcggcagt
     1081 tctctaggag cgacgcggcc acgtgcttca gaacttcgtc tccgacctga tgtccgtgct
     1141 tgtcgttgag gtccttgaac ttgtcgacgt cgagcatgac cagggtcacc tggtcgccca
     1201 tccgctgtgc tcgggagagc tccttctcga gcatcgtttg gaaggtacgg cggttgggga
     1261 tccccgtaag gccgtcggtg tcggccatcc ggcggatctc ctcgaccagc caggcgtttc
     1321 gaagtgcgag cgcaccgtgg gctgcgaact gcgacaccat gtcgatcgtg cggcgctcta
     1381 tccggtgctg cttcctggac gcgtattcca gcgccaccac cccgatcggg ctgccgtcgg
     1441 cgaacatcgg tgcgacgacc aggctcttcg cgaacgggag cagggcggtg agctggggat
     1501 ctgactcggc gttcagcttc ttcagtaact gtggcttgcg ctccgaccag gcccgcgcga
     1561 tggcctcgtc gagatggggc ttgacctctg ccggctctcc gggccccttg tatgcaagta
     1621 gggcggggtc gagtgctcct tcggtggcga gaacggtccc acggtcgaac tcgaacgtat
     1681 cgatcaatcc atccagcagc gcctgggcaa catcgcgcgg cgattgcgag tcgtcgagtt
     1741 tctcggcggt tcgcgcgagg gactcgagat cgctctggtt acgacggagc tgccgctcgt
     1801 tgatggatga gaaagcggtg gttaccaggg cgacgagcca gaaggcaacg acgttgaaga
     1861 acgacggctt ctggaagcca cctccggtct ccgttgcggc ctcgagtgga gcgatgatgc
     1921 gcgctgcttg tgcgtagaac acgacgaaga aaagcaacga atgccacagc gcgatcttca
     1981 gccccgtccg gtgcgaagca agcagagtca cagcgatgag gtggaggtaa acgaggaacc
     2041 ggagcggact ttgcgtcccg ccggtctgat aggtgaccca tgcgagaaag aggccgtcca
     2101 tgatcagcat gccgccgacg gccgtcaggc ctcgtcccga accggcgctc ctcactcctt
     2161 cgaccgcggc cgacagcagc aggtagccgg ccgtgacgaa catcacatcg tcgagtccgg
     2221 cccccacgac gtccgggacg agaacggttg atgcgaggac gatggcagct agggcggcgc
     2281 ggagggcctg caggtacccc atccgctctc ctagagaggc gatctcgacg gagcgcggat
     2341 ggttctgcga tcgagttgta ctcatctatt cgaagtacag cacttcttcc tccggatcga
     2401 caggcgcgag cgagagtttg gggtcgcgtc tatctacccg accgtgaacg gcgaggaagg
     2461 cgaggacgat cccaaggagc gctacgggga aggcgacgcg gcgggcggtc tcggccgcct
     2521 ggtccgccag gtcgtgcaag aagctgtcga ccgcgttgtc ggggtacctc tcgctcgtca
     2581 cgaaacgccc cggccaggcg ggattcacct gcggggcagc ggcctcgcgc gaaccggcgc
     2641 cggcactgtt ggatcgttcc gtgcgcgtcg ccgacggcct ccgggaggcg gcaccttcgt
     2701 gtccgccgag gccgacctgc gtcgcgcccc tgctcgaggg gtatgagcgc attaggtagg
     2761 ggagggaaga agctggggac gctcccgatg tgaccgatcc cgagccttca ccggacacgc
     2821 tgcgagcgcc ggacggctta cctacacgcg gggccgcgtc cgggtcgccc ggaacctggt
     2881 ccgtggggtt ggtagcagga tcggaaccgc cggtcccacc gccaccccca ccgatgacgc
     2941 cgtcgaccgt gtcgttcgcg tcctccacta cctcgtcgac ggtctcgtcg accgcatctt
     3001 gtgtgtcgtc gatcgtctcg ccgacctcgt ccgtgacctc gtcgacggtc tcgtccactt
     3061 cctcctcgac gtcgtcgata acgtcgtcca cgacatcggt cacgtcgtcc gtagcgcctc
     3121 ccgtggcgtc gtcaacctcg tcgcccacgt cggagacagc gtcgccggca gcatcggtgg
     3181 cgtcatcgac ggcgtcggag gcctgatcgc cggcatccga gagcgtgtcc gaaacgtggt
     3241 cggtggcatc gtcgaccgag tcggttgcat cgcccaccgc gtcggaggca tcatccccga
     3301 cgtcccccag agggtcggcc atcgcgccgg ccgggatcag cagacagagc agaaccattg
     3361 cgagcacaac tcggcgtgcg taggacttag ggcgactctc ttccacctgg ccacctacct
     3421 cttcgttgaa gacggttccg cctcttggtc tatcggcagg tactagttgc cccccgccta
     3481 gtcagaacgg gctatcttgg gcatagccca tctgggctac ccggctaccg gccctggagg
     3541 gggaatttcc gcctcggatg gccgtgcgag gcggggatat ggcttatagg gtagcgagcc
     3601 tatagaaggc agtgcttcta ggacacagag gagggacggc ttgaacaaga aggaactcat
     3661 cgactcgata gccgacaaga cgggtgaaag caagcgcacg gtcaacgaag tcctggacac
     3721 gacgatcacg cagatccagt cgcaggtgaa gaagggcgag cgggtgtctc tgcctggctt
     3781 cggaaccttc gagcgtcgca atcgttccgc ccgtacggcg cgtaacccgc gcacgggcga
     3841 ggagatcaag gttccggcga ccaaggtgcc ggcgttcaag cccggttcga gcttcaagga
     3901 cttcgtagcg ggccgttagt tcgctacgct ggtcgcatca catagaagcc ccggctccgg
     3961 ccggggcttc attgcatcaa gcagcggcct cggccggggc ttcatcgtcg atcaagcagc
     4021 ggcctgggcc ggggcttcat catcgattgg ttagcctctc ggcgtgaaga gagcagcttt
     4081 gggaggagct catcggggtc ccgtggacgc atcgcgtcaa cgccggacga tccgcctggt
     4141 acagatcctt ctggtgttga tctcggcggg cctgctgatg ttcgccggct actcgttcgg
     4201 ccgcgtcagc ggctacagga gcgctcgttc cggagccgtt ttcgaacgac ctcggccccc
     4261 gtcgcccgcg caacccatcg ttctgacgat cctgggcggg atcgcgatcg cgggcgcggc
     4321 agcactgcaa caagggtcgt cggtccgcat gcccacaccg gcacggctgg acgagttggc
     4381 cggccgcgcc gaacagaccg cgctcgagag ggcagagaag gcagaagagg cagagaaggc
     4441 gtagcgggcg cgactaactc tctgcgccgt tcacatgcgg acgcagtttc gcaagaatct
     4501 ccgcactctt ggactcaccc gttcgttccc tcgttaggaa ctcatccacg tcttcgatcg
     4561 tgtcgatgtc caatgcgaga cgtgggagga cgaggatcga acaccggaac ccgagccgtt
     4621 ccgccagcat caggtgtgcc tgcatgcttg cggtgccgaa ccttggagcg aggatgtcgg
     4681 gcggccgcag gtaaagacca ttcgtgcctc cgtcacgagc agagggaacg acgactactt
     4741 ccgacgtcga tcctgtgtcg aggaggtcgc gcagatcacc agagtatgcg agtggaatgt
     4801 cggaggggat catcgtcacc gaagtcgcgc cttcggccat cgccaccaga accgcttgtt
     4861 gcagggcacc gttcagtgtt cccgtctggt ccttcacggt gttgaagcca taatcgtgag
     4921 cgcgcgtcag cgcctcttcg tcatcactgg caacccacca ggtaaggaag ctcgcatccc
     4981 ggcacaaggc gagtgagtcc tccagtagcg cccgggcgac ctcgagccgg tcggcatcgt
     5041 cgagatgctc ggccaggcgg tgcttggcac gagagaggtt cttgacgggc aatatccctg
     5101 cgtccatgtc cttaaggtac ccgcccaccc ccgcgcggcg gcccccgccc gccggatacg
     5161 atgccgcctc tcatggacaa gagcgcgcgg aaacattgga agcacctcta cggcgaggtc
     5221 gtagccacag agatctgctg tggttgcgcc gcctgcgtcg tcgcctgccc gcacaaggtc
     5281 ctcgagatgc aggacttcga tccgatccag acggacatcc tgtctccgtt cgacaactgc
     5341 gttcacgggg aggagggctg ctccctgtgc gccatggctt gccttcggct cgaaccggcg
     5401 ctggacgcga tcgagacgtt ggtcttcggg gcccgacgga agagtgacca acccgagggc
     5461 gcgtaccgct ccaagacact cgctcgcgcg accgacgagc gcatcatttc ccggggtcag
     5521 gacggaggcg cggtaacggc tttgctcgcg tggggcttgg accagggtga attggatggg
     5581 gccgtcgttg cctctccctc ggccaccgtc ccatggctgg acgagcctct gctcgtccgt
     5641 agctcagagg agctcctggg ggcggccggg tctcgttaca cgtactgctc gacacccctg
     5701 gggcttcgtc aggctgccgc cgcgaagtgc aagaacgtcg ccctcgtcgg ggtgtcgtgc
     5761 gagtccaccg ccgttaggga gctcaccgcc gagggcatca agcgatggac gcggccggtg
     5821 aagctggtca taggtctcat gtgctgcgag accttcgact acgaggcatt catgatcggc
     5881 aaggtccaac aggagctggg catctccttg gatgacatcg tcaaggtgaa cgtcaagggg
     5941 aaggtgatcg tgaccttgaa agacggtcgc gacatcgaca tccccttgaa ggacgcgcga
     6001 ccgttcgcca atgagtggtg ccatcactgt cccgacttcg ctgcagagca cgcggacctc
     6061 tcctgcggtg ggctggggat ggaaggttgg acgatgatcc tcgtccgatc gcagaccgga
     6121 caggactatc tcgaacgagc ggtcgaggcc ggcgtgttgg agcttcgccc ggcggaggag
     6181 gagcccaagg cgctggaggt tatggacagg ctcgcacgca agcagcgaga gcggatcgat
     6241 ccctgggatc cgcacgcggc tacgaggtgg cccacggagc aggcgttgaa cgccgctcga
     6301 gcggaggcgg cggcagaagg ggcaggctag gtcactcggt cagttcgaag tgtctgacga
     6361 tcggagcctg ctggaacagc ttgcgtatct cttcgagagc ttccttgtaa tcgtcgctcg
     6421 tcgttgcttg ctcgatggac tctatggagt cccaccgcga gatcgacgcg atgtccacga
     6481 ggtcctggga gtgctcctcg acgccgatga ccatctcgac cccgtggcag ccggcgaacg
     6541 aatcgaacgc aggacgaacc ttctgacgga atagctcctt gcccttctcc acatctgccg
     6601 ggtccattgc cgtgtcgaag atgcgaacga tcacgtgatc cacctttcca ttttccgccg
     6661 atggccgagc ccaccggcgt tcgacggact tgtcagtttt ggggtcatac gttccacgct
     6721 cctctgttga acatgcccgc gagctgggga ttaagtttta gttcacgttc cttgaacgtg
     6781 tagttggccg cgacgatctc tcccagaccc ggatagcgct gaacgaccct gacgcggtcc
     6841 tctcccacct cgatcacgtt gtaggagggc cgcgtgtatc cgcgcacgcg gtggctggag
     6901 gcggtgcccg agttgatcaa gagcatccgg ccgaactgcc acacgttcgg cacgtgcttg
     6961 tgcccggaca tcacgaggtc gaccttgagc tcggtgagta aagcgagtag gtcacctgcg
     7021 tcccacacga tgtttctttc acggccggtc ccagggaccg acacgagatg gtgatgcaag
     7081 acgaagatcc gatagtcgtc cggccattcg tagcaagccc ggatccagtc gtaacggatg
     7141 tgcccgacct caccgtcggc caggtccggc ttggaggagt tgacggccac gagggtggcg
     7201 taccgctgcg gttgtcctcc gcccggggga cagaagggca cccggaccac gcgatccgac
     7261 ttcccgaact gatccctgaa atggaggaac cccacgttca tgaggtcgtg gttccccggg
     7321 atcaccacca tctcgagatc cccgagacga tcgagatagc gcttggcctg gcggaactgg
     7381 ggggcgtacc cctccgaggt caggtctccc gcgcacacga cgagatcggg atcgagatcg
     7441 acgatctcgt ccacgacgtg ttccatcagc tcggcatcga acatcggatg cccgcagtgg
     7501 atgtcggaga actgtgcgat ggtgagccgg tcgccttcca cgtttccctt tcgccgacgg
     7561 cccagcccgt ctgttcgtcg ccgctggccg agcccagcgg cgttcgagac cgatcgtgtg
     7621 cgcgtccgta atatagatgc ggaggaggac tgggggaggg aatgccgatg gagccgacat
     7681 acgcactagc cagggcggtc ttgaagccgt ggctggggac gtggttccgc tggcacctgg
     7741 aagggatgga gaacatcccc cggagcggcc ccgccctgct ggccatcaat cacatcgcgt
     7801 atctcgatcc cctcgcggcc gcgtacgtgg tggacaaggc gaagcggatc ccgaggttcc
     7861 tggcgaagtc cgagctattc gacgacaagc ggatcgcatg ggtgctcagg ggagcaggac
     7921 agatcccggt caagcgtgga agctcggacg ctcctatggc tctcgatcat gcgatcgaag
     7981 ctctccggga gggagagatc gtggtcatct tcccggaagg aacgatcact cgggacccgg
     8041 atctgaatcc catggaaggc aagaccggga tcgctcgtct cgccctcacc accaaagcgc
     8101 ccgtcatacc ctgcgcactg tgggggaccg ccaacatctg gccgaaggga tacggcaaac
     8161 gatggagacc tcggcaggac atcttggtgc gcgtgggaga gccgatgcat ctcctcggtg
     8221 atcccgagtc gcgtgatgac tggagcgaag cggtccagcg cgtcatggat cggatttccc
     8281 ttctgctggc gagtctgcgt cccgctattc ccgaccgccg acgtccgcgc cggtcggctg
     8341 catgagcaag gtcgcgttcg tgggagcggg ttcgtggggt accgcgacgg ccgcgctcgt
     8401 cgctcggaag aacaacgccg aggcagtgct ctgggcgcgt cggggggaac tcgcagaaac
     8461 gatcaatctg tttcacgaga accccgaata cctgtccggc atcaagcttc ccgagaacct
     8521 tcgggcgacg aacgacctcg aggatgcgtt ggagggtgcc gacacggtcg tgatgggggt
     8581 gccctctcac gggttccggg aggtattgcg tgaggtcgct ccggttgccg gtcgtgatgt
     8641 ctgctacgtg agtctcacca agggcctgga gatagacacc cgcagccgga tgtcgcaggt
     8701 cctcgcagaa gaggtcgacg ggatcgatca gtccaacatc gccgtcctta ccggcccgaa
     8761 cctggcacga gaggtcgtgc aggatttccc cgccgcctcc accgttgcct gtaacgacga
     8821 tgccacggca gcccggttgc aggagatctt ccacaccccg accttcaggg cgtacacgaa
     8881 tcacgacgtt gtcggctgcg agctcggcgg agctttcaag aacgtgatcg cgatagcggc
     8941 gggcatcgct gatggactcg gtttcggcga caacacgaaa gcgacggtca tgacacgcgg
     9001 tcttgcggag atggctcggt tctcggtcaa gttcggggct cagccgatca cgttcctggg
     9061 tctcgccggc gtcggcgatc tcatcgcaac ctgcgccagt ccacagtcgc ggaaccactc
     9121 cgtcggtatc gaactcggga aaggcaggaa gatcgacgac atcatcgagt cgatgaacat
     9181 ggtcgccgag ggggtcaagt cctgcaagcc catcttcgag ttggggcagg aagccgacgt
     9241 gtggatgccg ttgacggaga acgtcaagcg gatctgtcac gacggagtga gtgtggaaga
     9301 ggtagtcagc gatctgctgg cacgagagat cagatcggag tttgccggga tcgaggagta
     9361 cctaacgttc aacgaacagg acgtcaggtc ctagggacta tccggctcgg tcgagcgctt
     9421 ccaccagatc ggtgatgagg tcttcgggat cctcgatccc gaccgacaac ctacagaacc
     9481 cctcggagat acccgcggcc gcgagctgct cggcgtcgag ttgggtgtgg gtgacgcttg
     9541 ccgcgtgaac gattagggag cgcgtgccgc cgagtgaagc ggcgcgctgt acgaccttga
     9601 gctcctcctg gaagcgaccg gcttgctctt tccccccggc tacatcgaac gcgagcatcc
     9661 cgccgccgta gccgcccaag agcctctctg cgagggcacg tcctgggtca ccgtccagca
     9721 tcgggtagaa gacggccgac acaccggggt gttcggccag agcctgggcg acgaggagag
     9781 ctgattcgct cgctcgcagg acccggagat ggatcgtcgc gataccacgc agggccagcc
     9841 aggcattgaa aggcgacagc accgccccga actcccgagc gatgtgacgc atccgctcga
     9901 tgactccggg ctcggcgcag acgacgcctc cgatcaggtc gttatgcccg ccgaggaact
     9961 tggtcgcgga atggatcgac gcggtggcgc cgtgctcgag cggccggcag agcaccgggc
    10021 ttgcgaacgt gttgtcgact aggagtggaa cgcctgcagc gcgcgcgagc ttggcgagcg
    10081 cagggatgtc ggcgacctgg acgcgagggt ttccgatcgt ctcgcagtag agcagcttcg
    10141 ctccatcgag agcgtcttcg atggacgcgt ggtcggccac gtcacagaat gttgtttgga
    10201 tcccgtaacg gggaagcgtt tgcgataaca gctcgaaggt tcctccgtag agctgccgcg
    10261 ccgccacgat ccgatccccc gcagaacatt ccgccagaag caccgtggag atggcggcca
    10321 tcccagaagc gaaggcttct gcgtcttggg cgccttcgag atcggccaca gcagcttcga
    10381 atgcatcaac ggtcgggttc gcccaccgcg tgtagacgta gccggcgccg accttctctt
    10441 ccgaagcctt cgcgaacgac tcggtggaat cgaatgcgta tgtggacgcg aggtcgagca
    10501 cgggcgcgac cggctcgcct tcttgccggg gagggcgcgg tgggtgaacg gctctcgttc
    10561 cgatcccgtg cggccgcttg cggctcacgc cgcgggccta gagatggacg acggggcagg
    10621 tgagcgtgcg cgtgatcccc tcgacggctt ggatcttcgc aactacgagc ttgcctagat
    10681 cgtcgacgtt cgatgcgctc gcgcgcacga tgacgtcgta agggccggtt acatcctcgg
    10741 ccgcgtcgac accgtcgatg ctgcgcacct ctttggctac ctgcgcagcc ttgccaactt
    10801 cggtctggat gagaatgtag gcctgcacgt cagccaatgt cgcctccttg ttggggcaca
    10861 acctgtggtt ccgtgcgccc gtgtacgggt tgagggagtg tagcaaaaga gcttgagagc
    10921 cctctgagga cccgtgattg ttaggcttgc cttttcccca gcgataggtt tccagaaaga
    10981 ggagtccaga agttgtcttc aacagcgcca gccggagtga ccgaccccgg caaggtattc
    11041 gtcaacctca gccccccgca tctcgtcgag cactctctgg aacgcaacga gggcatattg
    11101 gaacgctcgg gggcacttgt tactcgaacg gggacgcgaa ccggccgttc tcccggcgat
    11161 cgcttcttcg tctcccatgg cccctcgaaa gagcagatcg actggggccc cgtcaataag
    11221 cccttcgagc cgagttcgtt cgaggcgctt ttcgaccggg tgcgtcggca cctgcgcggc
    11281 cgcgagctgt acgtcgtcga cgggttgctg ggggccgatc cgacacatca gatcaagctc
    11341 cgggtcgttt gcgaacggcc gtggcatgca ttattcggca ggcagttgtt ccgtcgtccc
    11401 agtgaagaac aactgagaac cttcgaaccc gagttcacgc tgctggcggc acccagtttc
    11461 gaggccgtgc cggaccgcga cgggacgaac tcggaagcgt tcatcggcat cgatctcgag
    11521 tcgaagcagg ttctcatctg tggaacggaa tacgccggcg agatgaagaa atcgatgttc
    11581 acatccgcca actacctgtt tccccagcag ggtgtgctcc cgatgcattg ctcggccaac
    11641 gtcgacgagg aaggcgacgt ggcgctcttc ttcggtctgt ctggaaccgg aaagaccacg
    11701 ctttcggcag acgcgcaccg ccgactgatc ggcgacgacg agcacggttg gtcggactcc
    11761 gggatattca acttcgaggg cggttgctat gcgaaatgca tcaatctctc gcgcgaacgg
    11821 gagccccaga tctgggccgc gatcaagttc gggtcggtcg tcgagaacgt ggtcgtcgac
    11881 gaggcgacac ggatcgtcga ttacgacgac gacgcgataa ccgagaacac ccgcgccgcg
    11941 taccctctcg acttcatccc cggtttcgtc cccgagggac gcgccggtca cgcgaacacg
    12001 gtcgttttcc tgacggcgga tgccttcgga gttctcccgc cgatctctcg cctcggccct
    12061 gagggggcga tgtatcactt cctgtcgggc ttcacctcga agctcgcggg gaccgaagcc
    12121 ggtgtcggaa gcgagcctca ggcgaccttc tccacgtgct tcggcgcgcc gttcctaccg
    12181 ctcaagccgt ccgtctacgc agagatgc
//
LOCUS       DATMED010000337         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4118347, whole genome shotgun sequence.
ACCESSION   DATMED010000337 DATMED010000000
VERSION     DATMED010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4118347"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VNP73_08755"
     CDS             <1..485
                     /locus_tag="VNP73_08755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWL66048.1"
                     /translation="VDDGMTFPVLRTNWYSVINFRDDWHAPRMRLVGGKWRQVGIHEG
                     NDIFAEPGTPIVAMMPGRVEQIGWTFYSGWRVGVRGIDSNYWFYAHMRRFAPGVGIGT
                     PVEPGDTLGEVGNTGYGLEPGHHDEFPPHLHVGIQRPDGAWVNPYPLLKKLYAASLRA
                     "
     gene            517..1563
                     /locus_tag="VNP73_08760"
     CDS             517..1563
                     /locus_tag="VNP73_08760"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWL66049.1"
                     /translation="MTELIRPGDHNEKVKDVQSRLRSLGHDIVDAAGYFGGTTKRAVR
                     AFQQQREILIDGIVGPNTWNELVEASWRLGDRTLYLRHPYLRGDDVNTLQARLNALGF
                     DSGREDGIFGPETDVAVRAFQKEYATAEDGIFGPASHAALAGLRVDRPGTSAKLREEL
                     KKDLGPRLEQAVVVIDPGHGGTDPGEVGTRGNREADLCWELSTRLAEQLSIEGARVRF
                     THTEAEAPDSSERARRANELQGDILVSIHLNSHEEWTAEGASTYFFGGSHAGQTLAEK
                     ILDELVMLGARDCRSHARSYTLLRETRMPAVLVEPAFISNPDEEKKLEDSDHLNVLAS
                     SIVAAIRRYHEESL"
     gene            complement(1542..2471)
                     /locus_tag="VNP73_08765"
     CDS             complement(1542..2471)
                     /locus_tag="VNP73_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWL66050.1"
                     /translation="MPKRSGLGRGLDAILPTARAEATSVDGGPLREIPIGSIDPNPRQ
                     PRREFDAASIAELATSITEVGLLQPLLVRPVSAERFELIAGERRLRAARSVGLEAVPA
                     LVVETDERGSLERALVENIHREDLNPLEEAAAYKQLMEDAGLTQEGLGERLGRSQSAI
                     SNSLRLLDLPVLLQRMLTEDQLTGGHARALLALQGHPMQERAARSVATDGLSVRATEA
                     LVRRYQEMFGSSSSKKMGRPREISAAALEAQNRLSDHLQTKVKVEASKRKRRIIIDTT
                     SDEELNRIVEVILGDEVGGVPTTVGPGQSDSSW"
     gene            complement(2472..>3333)
                     /locus_tag="VNP73_08770"
     CDS             complement(2472..>3333)
                     /locus_tag="VNP73_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWL66051.1"
                     /translation="LGAALALRGHRVLLVDIDPQANATTGVGLDHRSVELSTYDLLTG
                     DAGLGQVTQPTEIKNLDCAPAAVDLAGAEIELTAVMDRERKLAEALVSARERYDLVFI
                     DCPPSLGLLTVNALTAAEDLIVPVQCEYYALEGLGQLVETAERVRSALNPELRIAGFL
                     MTMYDARTKLSQQVMDEVRNHFGDLVFETVIPRSVRLSEAPSFGEPVLTLDPSSRGAV
                     SYRLLAVEVEERYGLPVHIPPPPAPAEVREPVSVGAGTPGPAGRGFGTVSVEPDGLDN
                     AWPIEPWRIL"
BASE COUNT          614 a         1076 c         1066 g          577 t
ORIGIN      
        1 tcgtcgacga cgggatgacg ttccccgtcc tgcggaccaa ctggtactcg gttatcaact
       61 tccgcgatga ctggcatgcg cctcgcatgc gcctcgtcgg cggcaagtgg cgtcaggtgg
      121 ggatccacga gggcaacgac atctttgcgg aacccggcac tccgatcgtt gcgatgatgc
      181 ccggccgcgt cgagcagatc gggtggacct tctattcggg atggcgggtc ggcgttcgcg
      241 ggatcgacag caattactgg ttctacgcgc acatgcgccg gttcgcaccc ggcgtgggca
      301 taggcactcc ggtcgagccg ggcgacaccc tgggagaagt agggaatacc ggctacgggc
      361 ttgagcccgg ccaccacgac gagttcccgc cccatctcca cgtcgggata cagcgacccg
      421 atggagcctg ggtcaaccca taccccttgc tcaagaagct ctatgcggct tcactacgcg
      481 cctgatgtca gagctcatcc agtgggactc gaacggatga ccgagctcat ccgtcccggc
      541 gatcacaacg agaaagtgaa agacgtccaa tcgcgcttgc gctcgctcgg ccacgacatc
      601 gtcgatgccg ccggttactt cggggggacc accaagcggg ccgtgcgtgc tttccagcag
      661 cagcgcgaga tcctcatcga cgggatcgtg ggacccaaca catggaacga gctggtcgaa
      721 gcctcgtggc ggctcggcga ccggacgctc tacctccggc acccttacct caggggggac
      781 gacgtgaaca cgctgcaggc tcgactcaac gccctcggtt tcgactccgg gcgcgaggac
      841 gggatcttcg gacccgaaac agacgttgcg gtgcgcgcgt ttcagaagga gtacgcgacc
      901 gcagaggacg ggatcttcgg gcccgcctcc cacgcggccc tagcaggact gcgcgtcgac
      961 cggccgggca cctccgcgaa gttacgcgag gagctgaaga aggatctcgg cccgcgcctc
     1021 gagcaagccg ttgtcgtgat cgaccccgga catggaggca ccgaccccgg cgaggtgggc
     1081 acacgcggca accgcgaagc agacctgtgt tgggaactct ccacccggct cgccgagcaa
     1141 ctctcgatcg agggggcgcg ggtgaggttc acccataccg aagccgaggc acccgattcg
     1201 agtgagcgcg cgcgccgggc gaacgaactc cagggggaca tcctggtctc gatccatctg
     1261 aactcgcacg aagagtggac ggccgaagga gcgtccacat atttcttcgg tggctcgcat
     1321 gccggacaaa ctttggccga gaagatcctc gatgagctcg tgatgctggg tgctagggac
     1381 tgtcgcagtc atgcacggtc ttacacgctg cttcgagaaa cccggatgcc ggccgtacta
     1441 gttgagccgg cgttcatctc gaacccggac gaggaaaaga agttagagga ctcggatcac
     1501 ttgaacgtgc tcgcgtcctc gatcgttgcg gcgatcaggc gctaccacga agagtcgctc
     1561 tgacccggcc ccacggtcgt cggcacgccc cccacctcgt cacccaagat cacttcgacg
     1621 atcctgttca gctcctcgtc cgaagtcgtg tcgatgatga tgcggcgttt gcgcttcgag
     1681 gcctcgacct tgaccttcgt ttggaggtgg tccgacagcc tgttctgagc ttcgagtgcg
     1741 gcagccgaga tctcgcgcgg acgacccatc ttcttgctcg aactggaacc gaacatctct
     1801 tgatatcgac gcaccagggc ttccgtcgcg cgcaccgaca atccgtctgt cgccacggag
     1861 cgagccgctc gctcctgcat gggatgtcct tggagagcta ggagagcgcg ggcgtgcccg
     1921 cccgtgagct ggtcctcggt caacatgcgc tgcaggagca ccggcagatc gaggagtcgt
     1981 agcgagttcg agatcgccga ctgactccga cccagccgtt ctccgagacc ttcctgcgtt
     2041 agtccggcat cctccatcag ttgtttgtac gccgctgctt cttcgagcgg attgaggtcc
     2101 tcgcgatgga tgttctcgac cagagcacgc tcgagtgatc cgcgctcatc cgtttcgacg
     2161 accaacgcgg gcactgcctc gaggcctacc gagcgcgccg cgcgcaaccg ccgctcgccg
     2221 gcgatcaatt cgaagcgttc cgccgatacc ggccgcacca gtagaggttg caagagtccg
     2281 acttcggtga tggaagtagc gagctctgcg atcgacgcgg cgtcgaactc gcgtcgtggc
     2341 tgacgtgggt ttgggtcgat cgagccgatc ggaatctcgc gcagcgggcc accatcgacc
     2401 gaagtagcct cggcgcgcgc tgtcggcaga atagcgtcca gcccccggcc gaggcccgaa
     2461 cgtttcggca tttagaggat cctccagggt tcgatgggcc acgcgttatc gaggccgtcg
     2521 ggctccaccg acacggtccc gaaaccgcga ccggccggtc cgggtgtgcc ggcaccgacg
     2581 ctcaccggct cgcgcacttc ggccggagcc ggaggaggag ggatgtgcac aggcaaaccg
     2641 tagcgttctt caacctcgac cgcgaggaga cggtacgaaa ccgcgccgcg tgaagacgga
     2701 tcgagcgtca gcaccggttc cccaaaagag ggtgcctcgg acaggcgaac acttctgggg
     2761 atcacggttt cgaacacgag atcgccgaag tgattcctca cttcatccat tacttgctgc
     2821 gacaacttcg tgcgcgcgtc gtacatcgtc atcaggaatc ccgcgatccg aagttcggga
     2881 ttgagtgcgc tgcgcacgcg ctcggcggtc tctacgagct gacccaaccc ttcgagcgcg
     2941 tagtactcgc attgcacggg aacgatgagg tcctcggccg cggtcagtgc attgaccgtg
     3001 agcagcccga gagacggagg gcagtcgatg aacacgaggt cgtaacgctc gcgcgccgag
     3061 acgagggcct ccgcgagctt gcgctctcga tccatgaccg cggtgagttc gatctcggcc
     3121 ccggcgaggt cgacggccgc gggcgcgcaa tcgagattct tgatctcggt cggctgggtg
     3181 acctggccca aacccgcgtc cccggtcagg aggtcatagg tcgagagctc gaccgaccga
     3241 tggtcgagtc ccaccccggt cgtcgcgttc gcctgaggat cgatgtcgac gaggagcacg
     3301 cgatgccctc gaagagcgag ggcggccccc agg
//
LOCUS       DATMED010000338         3530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_286053, whole genome shotgun sequence.
ACCESSION   DATMED010000338 DATMED010000000
VERSION     DATMED010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286053"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNP73_08775"
     CDS             complement(<1..773)
                     /locus_tag="VNP73_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWL66052.1"
                     /translation="MPSPREKQVLVKVVATSINLSDWEGLRGNPMYARIGGLRAPRQR
                     TLGSDIAGQVEAVGSGVTRFRPGDEVYGDNLALMGGFAEYAVASESALAHKPKALTFA
                     QASTLPQAGAIALQGTASLRPGQRLLINGGGGGSGSFAIQLAKHAGAYVIGVDNAHKL
                     DFMRSLGADEVIDYQSQDFTRIEPCDLVLDLVAHRSVFAYRRVVARGGRYLCVGGSVR
                     ALLRIVTIGALLGRLTGRRMGILIVKQGPTSFEAFAERC"
     gene            1132..2271
                     /locus_tag="VNP73_08780"
     CDS             1132..2271
                     /locus_tag="VNP73_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66053.1"
                     /translation="MPIRTLTLQELRRDLAIRDLTDPEDGAHAIQLIVDQAVDALSSW
                     RCEVRRIRANPVVSAADNYDRLRIDTEAVSRETRYTRYVGEGLMLRSHTSVMIPPALR
                     DLAQRPSKDVLLACAGMVYRRDSIDRLHSGTPHQLDLWRVRERPPLTVTDLDEMVDRL
                     VGTLTPGRPHRSLSREHPYTTHGRQVDVQIGSEWVEIAECGLAHPEVLADAGLRPWTG
                     LAMGLGLDRLLMIRKGVPDIRLLCLNDRRIASQMLDLEPYREVSSYPPIRRHLSIAVG
                     ATDDAEELGDRVRVALGQNSQSVETIRVLSETSTAELPASAVERLGLRPGQKNVLLEV
                     VLRDLERTLTDEDANQLRNRIYESLHQGDTTEGNSEHKATTSENG"
     gene            2345..3166
                     /locus_tag="VNP73_08785"
     CDS             2345..3166
                     /locus_tag="VNP73_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66054.1"
                     /translation="MWEHGEMVPHDKPEDAQPESRVENVVGLGAETVAGVVGAVLLTL
                     GIYVVVYSTLIYDKGGFDYAAGQLYGVGLALLGLVLTGWSLVRLWRMPSPFRGRGRTW
                     IAVPLVASALWFGLRVVEAVDAKELQDEVLAATAPVRHDFESDLNDAFRAWREEANLE
                     GSYLGNDREKWSLDPTGDGAVDVDPPIVRAIRDMGWSERGLSVFDTDGDLVVDQIEWS
                     PADGADVGNSWCIPVVFLEAQPNSEEFDGLYLVAYHQGVRGSCNVEPATNILSRI"
     gene            3246..>3530
                     /locus_tag="VNP73_08790"
     CDS             3246..>3530
                     /locus_tag="VNP73_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HWL66055.1"
                     /translation="MPRLQYKSFATPDEVRAFPMGRAEVVTLDESVVGRAVYEPGWRW
                     STAMPAIAGTANCQLHHLGYSISGVMHVVTGDNQMIDIPPASVYEIPPGHD"
BASE COUNT          678 a         1119 c         1070 g          663 t
ORIGIN      
        1 atgcaccgct cggcaaaggc ctcgaagctg gtcggaccct gcttgacaat caaaatgccc
       61 atgcgacgcc ccgtgagccg accgaggagg gccccgattg tcactatccg cagcagggct
      121 cggactgatc cgccgacgca caagtaacga ccgccgcgag ccacgacgcg tcgataagcg
      181 aacaccgagc ggtgggcgac gagatcgagc accaagtcgc aaggttcgat gcgggtgaag
      241 tcctggctct gatagtcgat cacctcgtcg gcaccgagcg accgcatgaa gtcgagtttg
      301 tgggcgttgt cgacgccaat gacgtacgcg ccggcgtgct tggcaagctg gatcgcgaat
      361 gaccccgagc caccgcctcc accgtttatc agcaaccgct gcccgggccg aaggctcgcg
      421 gttccctgca gcgcgatcgc gcccgcctga ggcagcgtcg aggcctgggc gaaggtcaac
      481 gccttcggct tgtgagcaag cgccgactcg gacgccaccg cgtactcggc gaagccaccc
      541 atcagggcga ggttgtcgcc gtacacctcg tcgccaggac ggaaccgggt aacaccggag
      601 ccgacagctt cgacctgtcc ggcgatatca gatccgagcg tccgctgcct cggggcgcgc
      661 aggcccccga tccgcgcgta catcggattc ccacgcagcc cctcccaatc ggaaaggttg
      721 atggacgtcg caaccacctt cacgagcacc tgcttctcgc ggggggaagg cattgggacc
      781 tcctcgatcc gcagcacctc aggcgggccg tatcggtcgc gcacgacagc cctcacaacg
      841 atccgcctct agatccggag ttggtggaaa cgggacatgc agcacagcgt aagcacgcga
      901 tttggtggac gaccccacgg atcgtgtcgc gagttgttcg ataaggactt cgttcgtcca
      961 aagacgaacc gatagactct ttgacatggc gattccgtac catcgattta tgcccgcccc
     1021 ggctggaaaa cggccggcgg tggggcgcgc gccattcgta gcctgaccgc ttaggacgtt
     1081 tccggctggg ggcctttccc ctcttcgcct ctccagccaa aggaaccgtc catgcctatt
     1141 cgaacgctca cactccaaga acttcgccgc gacttggcga ttcgcgatct caccgaccct
     1201 gaagacggag cccacgccat ccaactcatc gttgaccagg cggtggacgc gctgagctcg
     1261 tggcgatgcg aggtgcggcg aatccgtgcg aatccggtgg tctctgcggc cgacaactac
     1321 gaccgtctcc ggatcgatac cgaagcggtt agccgagaga ctcgctacac gcgctacgtc
     1381 ggcgaaggtc tgatgctcag atcccatacc tcggtcatga ttccaccggc cctgcgagat
     1441 ctggcacagc gtccgagcaa ggacgtgctg ttggcgtgcg ccggaatggt ctaccgtcgt
     1501 gattccatcg accgtctcca cagcggtacg ccccaccaac tcgacctctg gcgggtacgg
     1561 gagcgaccac ccctcaccgt caccgacctc gacgagatgg tggaccgtct cgtcgggacc
     1621 ctcacccctg gccgacctca tcgttcgctc tcccgagaac atccctacac aacccacgga
     1681 aggcaggtcg acgtgcaaat cggctccgag tgggtcgaga ttgcggagtg cggacttgcc
     1741 catccagagg tgctcgcgga cgcggggttg cggccttgga ctggtctcgc aatgggtctc
     1801 gggctcgacc gcctgctcat gatccgaaag ggggtgcccg acattcgcct cctgtgtttg
     1861 aacgaccggc ggatcgcctc acagatgctc gacctagagc cctaccgtga ggtttcttcc
     1921 tatccaccga tccggcgcca cttgtccatc gccgtcggcg ccacagacga cgcagaggag
     1981 ctcggcgacc gagtccgtgt ggcattggga cagaactccc agagtgtcga gaccattcgg
     2041 gttctctccg aaacttcgac tgcagagctc ccggcatctg cggtggagcg gctaggcctt
     2101 cgacctgggc agaaaaacgt gctgctcgaa gtcgttctgc gggacctgga gcgaacgctc
     2161 accgatgagg acgccaacca gctccggaac cgcatctacg aatctcttca tcaaggggac
     2221 acgaccgaag gaaactctga gcacaaagcg acgacctccg aaaacggatg aaagctggcg
     2281 cgccgtgcct ccaatcgact gatcctggca tttgcgagaa ccgtttcaag gttgaggttc
     2341 agtcgtgtgg gagcatggcg agatggttcc gcatgacaag ccagaagatg ctcagccgga
     2401 atctcgggta gagaacgtag tcggcctggg tgccgagacc gtggccggag tggtcggagc
     2461 cgttctccta accctgggaa tctacgtggt ggtctactcc actttgatat acgacaaagg
     2521 aggcttcgac tacgccgccg gtcagttgta cggtgtcggt ctcgcccttc tgggcctcgt
     2581 ccttaccgga tggtccctcg tccggctgtg gcgtatgccc tcgccttttc gaggtcgagg
     2641 ccgaacttgg atcgccgtgc cattggtcgc atctgccctc tggttcggac ttcgagtcgt
     2701 cgaagccgta gatgccaaag agctacaaga tgaggtgttg gccgcgacag ctcccgtgcg
     2761 tcatgacttc gaatcggacc tcaacgacgc ctttcgtgcg tggcgggagg aggcgaacct
     2821 cgaagggtct tacctcggaa acgatcggga gaagtggtca cttgacccaa ccggcgatgg
     2881 tgcggtcgac gttgatccgc cgattgtccg agctatacga gacatgggtt ggtccgagcg
     2941 tgggttgagc gtgttcgaca ccgatggcga tctagtggtc gatcagatcg agtggtcgcc
     3001 ggcagacggc gctgatgtcg gaaattcatg gtgcattcca gtggtcttcc ttgaggccca
     3061 acccaactcg gaagagttcg atggccttta cctcgtggcg taccaccaag gagtccgcgg
     3121 atcgtgcaac gtcgagccgg cgaccaacat tttgagccgc atctgaacac tcgatcccca
     3181 ggtctccaag cgtcgctatt ggcatcgatt ccagctctga aacggcgaat tattctgccg
     3241 tctgcatgcc tcggctgcag tacaagagct tcgcgacacc ggacgaggtt cgggcgtttc
     3301 cgatggggcg cgccgaggtc gtcaccctcg atgagtccgt ggtcggccga gccgtgtacg
     3361 aacccggttg gcggtggtcg actgctatgc cggcgatcgc tggtaccgct aactgtcagc
     3421 tccaccactt gggttactca atttccggcg tgatgcacgt cgtcacggga gacaaccaga
     3481 tgatcgacat tccgccggct tccgtctacg agatcccacc cggtcacgac
//
LOCUS       DATMED010000339         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4880103, whole genome shotgun sequence.
ACCESSION   DATMED010000339 DATMED010000000
VERSION     DATMED010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4880103"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VNP73_08795"
     CDS             complement(<1..993)
                     /locus_tag="VNP73_08795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWL66056.1"
                     /translation="MTEPLRVAVVGTGHVGLITCASLSAIGHTVIGMDSDEEKIVQLQ
                     AGGCPFFEPGLKELLAQQIDSGRLSFTRDIREAVRDADVAFICVGTPPRASGDASLVA
                     VEKAGRAIAKSATKDIVIVEKSTVPAGTAGRLDQVLAHERPDSHPILEVASNPEFLRE
                     GNAVQDSLEPDRILVGAHSDWARQRMRDLYEPLIDKGHRYIETDIATAEIAKHACNAF
                     LAMKISFTNALAQISERAGADVMDVAQVMGADPRIGPAFLNAGLGYGGYCFPKDLQAF
                     GRLAHRLGYDFPLLAEIERINEDAVATMFSKVQEKLWNLEDKRVCLLGLSFKPNT"
     gene            complement(996..>2000)
                     /locus_tag="VNP73_08800"
     CDS             complement(996..>2000)
                     /locus_tag="VNP73_08800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWL66057.1"
                     /translation="YAARRRQDGRLSFRTLVVGANDEAGDLMWALRFPDAGFTPIGYV
                     VGSDGRASANGLPVLGSVDDLVEIVRRESVECLFTATTALSSEDVIKVAVAARRTGAE
                     VRLSANLPQVLTTRATLQPVGDIMALSLTTPRLSGRQVVTKRVFDIVVSLFVATVTAP
                     LWLAGALAIKLTSTGPVFFRQDRVTKGGRTFTVYKLRTMAADGDRILEERGIDLTTPY
                     FKLDNDPRVGRLGSFLRRSSIDELPQLYNVIKGDMSLVGPRPLPADQVAANLEFLGPR
                     HEVPAGVTGWWQIQGRSEIAPEKAVEMDLFYIENWSLAFDLFILAKTIGAVTMRKGAS
                     "
BASE COUNT          363 a          630 c          636 g          371 t
ORIGIN      
        1 ggtgttcggc ttgaacgaga gacccagcag gcaaacccgc ttgtcctcga ggttccacag
       61 cttctcctgc accttggaga acatggtggc aacggcgtcc tcgttgatgc gctcgatttc
      121 ggccagcaga gggaagtcgt agccgagtct gtgggcgaga cgcccgaagg cctgaagatc
      181 cttgggaaag cagtacccgc cgtagccgag tcccgcattg aggaacgccg gtccgatgcg
      241 cggatcggca cccatcacct gtgccacgtc catgacgtcc gctcccgcgc gttccgagat
      301 ctgcgcaagg gcgttggtga acgagatctt cattgccaga aacgcgttgc atgcgtgctt
      361 cgcaatctcg gcggtcgcga tgtcggtctc gatgtacctg tgacccttgt cgatcagagg
      421 ttcgtagaga tcgcgcatcc tctggcgggc ccaatccgaa tgggctccga cgagaatgcg
      481 atcgggctcg agcgagtctt gcaccgcgtt tccctcacga aggaactccg ggttggaggc
      541 gacctcgaga atgggatgcg aatcgggccg ctcgtgggcg agcacctgat cgagcctgcc
      601 ggctgtgccc gcgggaaccg ttgacttctc gacgatgacg atgtccttgg tggcgctctt
      661 agcgatggct cgccccgcct tctcaaccgc aaccaggctt gcatccccgc tcgcgcgcgg
      721 gggcgtaccc acacagatga aggcgacgtc ggcgtccctc acggcctcac ggatgtcgcg
      781 agtgaaggac agccgacccg agtcgatctg ctgcgcgagc agttccttga gtccgggctc
      841 gaagaaagga cagccacccg cttggagctg cacgatcttc tcctcgtcgc tatccatgcc
      901 gatcaccgtg tgtcctatgg ccgacaggga cgcgcaggtg atgaggccga catgaccggt
      961 ccccactacc gcgactctga gaggctcggt cattcttacg atgccccctt gcgcatggtg
     1021 actgcgccga tcgtcttcgc caaaatgaat agatcgaaag cgagggacca gttctcgatg
     1081 tagaagaggt ccatctcgac tgccttctcg ggcgcgatct cgctgcggcc ctgaatctgc
     1141 caccaaccag tgacgcccgc gggcacttcg tggcgtggtc ccaggaactc gaggtttgcc
     1201 gccacctggt cggcggggag cggcctcgga cctacaagac tcatgtcgcc cttgatcacg
     1261 ttgtagagct gcggcagctc atcgatgctg gagcgtcgca ggaagctccc gagccgaccg
     1321 acgcgtggat cattgtccag cttgaagtag ggcgtcgtga gatcaatgcc gcgttcctca
     1381 aggatccgat ctccatcggc ggccattgtc cggagcttgt agacggtaaa cgtgcggccg
     1441 cccttggtca cgcggtcttg ccggaagaag accgggccgg tggaggtcag tttgatcgcg
     1501 agcgcaccgg cgagccacag cggcgccgtc acggttgcaa cgaacaggct cacgacgatg
     1561 tcgaagacac gcttcgtcac tacctgccgg ccggagagtc tcggtgttgt gagcgacagg
     1621 gccatgatgt cgccgaccgg ttggagcgtt gctctggtgg tgagaacctg cggcaagttc
     1681 gctgacaggc gcacctcggc gccggtgcgc cgcgcggcca ccgcgacctt gattacgtct
     1741 tcagaactga gcgcggtggt cgcggtgaag agacattcga cactctcccg gcggacgatc
     1801 tcgacgagat cgtcgacgga gccgaggacg gggagcccgt tcgcgctggc gcgaccgtcg
     1861 gagcccacca cgtatccgat cggagtgaag cctgcatccg ggaacctaag ggcccacatc
     1921 aggtcacctg cctcatcgtt cgctccgacc acgagcgtcc gaaaggacag ccgtccgtcc
     1981 tgcctgcgac gcgctgcata
//
LOCUS       DATMED010000340         4149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1814511, whole genome shotgun sequence.
ACCESSION   DATMED010000340 DATMED010000000
VERSION     DATMED010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814511"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VNP73_08805"
     CDS             complement(<1..1154)
                     /locus_tag="VNP73_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66058.1"
                     /translation="MTRRIPVVLLSAMFILSLTSSAPAAKVDKVQFALEGEARGLELA
                     LGDQGITLGLAMSRADSTPQALGVAAGQCAVLGDSPDPDNLPCNESTTEKSSSREAAG
                     EPGGTCASPQLPGELASVLTIEVACGSSVAELLTGKLPVTTNEGKVAEVGVGLDLSGI
                     VPQAEDAKEQLVDQLQEIINQAPEQVRNAVDQLLESVDEGQGAQIILGPASSNVTAKG
                     STLSVKSTASGARIGVVGIPNIDADGNPIPGTSQATEDGLIIVEVGTATANATINKVD
                     ASSDSSSTAAIVTVKVRDITKVEPTYTEISVAPGQTVTILQGTPAESTISAAAATNKN
                     SKGSAVAAADAVRLHLLKGVEGGLKLGLGRATAAASGSVQPAVQPRPPTRA"
     gene            complement(1292..2953)
                     /locus_tag="VNP73_08810"
     CDS             complement(1292..2953)
                     /locus_tag="VNP73_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL66059.1"
                     /translation="MGSPIDGLRQFAESVHPRTFIRLREAGAIGDPKGLLGFAGALPW
                     LIGRGQSLGIVSQMHSIVLGDKPAILDRNGEESWKDLDMRANQFARALRQKGIRGSER
                     VAILLRNGREMIEVALGAQKMGIVACPLNTWAKPKELKNTLESADPSLLVYDTKHAEQ
                     VEACAHEGIDLVFVGDKDKALEGSISYEDLIRDQSASPPPPFTKDRGSAKVVIHTSGT
                     TGTPKGAERNSSGAGLGALANLLGRVPYRRDDVIFCPAPLFHSFGLATFTFACALGST
                     MILPEKFDPEESLKLIEEHQATAASFIPVMIKRIVKLPDEVKKKYDLSSLRIVMASGS
                     AMSPELRKAALDLFGDVLYDLYGSTEIGWVTIATPEDMKSKPKTVGRPVDGIDVAIFS
                     DNGERLETGDTGELFVKSNMLFEGYTSGESRDERDGYMSIGDLGHQDEDGHIFIEGRA
                     DDMVVVGGENVYPIEIEELIESLDGVDEAAVLGVQDDEYGEVLAAFVVGSVSKDQVVE
                     SCKKDLASYKVPRRVEVLDELPRTSTGKVLKRDLIDSLKGADPLE"
     gene            complement(2953..3975)
                     /locus_tag="VNP73_08815"
     CDS             complement(2953..3975)
                     /locus_tag="VNP73_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWL66060.1"
                     /translation="MKLGLVLGGGGLVGMAYHAGVLKALEDCGIDVSNSDVIVGTSAG
                     SIIGSYLAAGWTQSDFFAYAQGEHPNAVRGEAEQEDEVRRIFTPLWSNPAERARRTVG
                     SLFTLASSRGYWSKASRGREPGSALKKGFPSGLYSTEETRRRLHEDLPETWPREGLFI
                     CSVEMYSGKRVPFGHPDAPVVPLPEAVLASTAIPGVFPPVNINGTRYVDGGAYSATSL
                     DLATDEGCDAIICVAPLGYKMDGVVTARDPKIWFPMLARSLFARALRREVLAAREKGV
                     AVMVLRPGTTELKTHGTNAMRHFDRATFTQETRESTLKVLEQASDDPALSIWRNSATP
                     TKERVS"
BASE COUNT          717 a         1294 c         1303 g          835 t
ORIGIN      
        1 gcacgtgtcg gcggccgggg ctgcaccgcg ggctgaacgc ttccgctggc agccgccgtt
       61 gctcgaccga gccccagctt cagtccaccc tcgacaccct tcagtaggtg aagccgaacg
      121 gcgtcggccg cagcaacagc cgagcccttc gagttcttgt tcgttgcagc tgcagccgag
      181 atcgtggatt cggcgggcgt tccctgaagg atcgtgacgg tctgtcccgg tgctaccgag
      241 atctcggtgt aggtcggctc gaccttcgtg atgtcgcgca ccttgaccgt aacgatggcg
      301 gcggtggagc tggaatcgga ggatgcgtcg actttgttga tcgttgcatt cgccgtcgcg
      361 gtaccgacct cgacgatgat caatccgtcc tcggttgcct gggaggttcc ggggatcgga
      421 tttccgtcgg cgtcgatgtt cgggataccc acgacaccga tgcgtgcacc ggacgccgtc
      481 gacttgacgg agagcgtgga gcctttggcg gtgacgttcg acgaagcagg acccaggatg
      541 atctgtgcac cttggccctc gtcaacggac tcgagcaatt ggtcgaccgc gttgcgaacc
      601 tgctcggggg cctggttgat gatctcttga agctggtcga cgagttgttc cttggcatcc
      661 tcggcctgcg gaacgatccc actgagatcg agtcctacgc cgacctctgc aaccttgccc
      721 tcgttcgtcg tgacgggcag cttgccggta agcagttctg caacggacga tccacacgcg
      781 acctcgatgg tcaggacact tgcgagctca cccgggagct gcgggctggc gcaggttccg
      841 cccggttcac ccgctgcctc gcggctagac gacttctcgg tcgtggattc attacaagga
      901 aggttgtcgg ggtcgggtga gtcaccaaga acggcacact gaccggcggc aacgccgagg
      961 gcttgcggag tggagtccgc gcgcgacatg gccaatccca aagtgatccc ttggtcgccc
     1021 aaagcaagct cgagaccgcg cgcttcgcct tcaagtgcga attgcacctt gtcgactttt
     1081 gccgcaggcg cgctgctggt cagagacagg atgaacattg cagaaagcag aacgacgggg
     1141 atccgtcttg tcactggggg gcctcctcta aagaactccg ctcaagccgg agtcgaatgt
     1201 atccgcgggg aggccgtttt ggcaacataa cgtggttatt gactaacaga ttgctgtttg
     1261 tcgggaatgt cccgatttcg agctactcgg gctactcgag cggatcggcg cctttcaggg
     1321 agtcgatcag atcccgcttc aggaccttgc ccgtgctggt ccggggaagc tcgtccagta
     1381 cctcgacccg ccggggaacc ttgtaggagg ccaggtcctt cttgcaggac tcgaccacct
     1441 ggtccttgga gacggacccc accacgaagg cggccaggac ctcgccgtac tcgtcgtctt
     1501 ggacccccag gaccgcggcc tcgtcgaccc cgtcgaggga ctcgatcagt tcctcgatct
     1561 cgatcggata gacgttctcg cccccgacga caaccatgtc gtcggcccgg ccctcgatga
     1621 agatgtggcc gtcctcgtcc tggtggccga ggtcaccgat ggacatgtag ccgtcgcgct
     1681 cgtccctgga ctcgcccgag gtgtagccct cgaacaacat gttgctcttc acgaacagct
     1741 cgcccgtatc gccggtctcg aggcgctcgc cgttatccga gaagatcgcg acgtcgatgc
     1801 cgtcaacggg tcgtccgacc gttttcggct tggacttcat gtcttcgggg gtggcgatcg
     1861 tgacccatcc gatctcggtc gacccgtaga ggtcgtacag gacgtccccg aacagatcga
     1921 gcgccgcctt gcgaagttcg ggagacatcg ccgaaccgct ggccatgact atccgcaggg
     1981 aagagaggtc gtacttcttc ttcacctcgt cgggcagctt gacgatgcgc ttgatcatga
     2041 ccgggatgaa cgaggcggcc gtagcctggt gctcttcgat caacttgagc gactcttccg
     2101 ggtcgaactt ctcgggcagg atcatcgtcg aaccgagcgc gcaagcgaat gtgaacgttg
     2161 ccaggccgaa cgagtggaat aggggggccg ggcagaagat gacgtcgtcg cgccggtagg
     2221 gcacgcggcc gagcaggttt gcaagcgcgc caagtccggc tcccgacgag ttgcgctctg
     2281 cgcccttcgg cgtccctgtc gtgcccgacg tgtggatgac caccttcgcc gaacctcgat
     2341 ccttggtgaa cgggggtggt ggcgacgcgg attggtccct gatcagatct tcgtacgaga
     2401 tagatccctc gagcgccttg tctttgtctc cgacgaaaac gaggtctatc ccttcgtgtg
     2461 cgcaagcctc gacctgttcg gcgtgtttgg tgtcgtaaac gagaagcgac ggatctgccg
     2521 actcgagcgt gttcttgagc tccttcggct ttgcccacgt gttcaacggg caggcaacga
     2581 tgcccatctt ctgtgcgccc agtgcgacct cgatcatctc gcgaccgttg cgaagcagga
     2641 tggcgacccg ttcgctaccg cggatgccct tctggcgaag cgcgcgagcg aactggttag
     2701 cgcgcatgtc gaggtctttc cacgactcct ccccgttgcg gtcgaggatc gcgggcttgt
     2761 ctccgaggac gatcgagtgc atctgggaga cgatgccgag cgactggcct ctgccgatca
     2821 gccacgggag tgcgccggcg aagccgagca gaccttttgg atctccgatg gctccggcct
     2881 cgcgcagccg aatgaaggtg cggggatgta ccgattcggc gaattgccgg agcccatcga
     2941 tcggtgatcc catcagctaa cccgctcttt cgttggcgtt gccgagttgc gccagatgga
     3001 cagagcggga tcgtcggagg cttgctcgag caccttgagg gtgctctcgc gcgtttcctg
     3061 cgtgaaagtc gcccggtcga aatgacgcat cgcgttagtg ccatgagtct tcagttcggt
     3121 ggtgcccggc cggaggacca tcaccgcgac tcccttctcg cgagccgcca gcacttcgcg
     3181 tctcagagct cgtgcgaaca gtgaacgagc gagcatcggg aaccagatct tcgggtcccg
     3241 ggctgtgacg accccgtcca tcttgtaacc gagcggggcc acgcagatga tcgcgtcgca
     3301 gccttcgtcg gtcgccagat cgagcgacgt cgcgctgtag gcgccgccat ccacgtaccg
     3361 cgtgccgttg atgttcacgg gagggaacac accggggatc gcagttgagg cgaggacggc
     3421 ttccggcagc ggaacgaccg gtgcgtccgg atggccgaaa ggaacacgct tgcccgagta
     3481 catctctacc gagcagataa agaggccctc gcgcggccag gtttccggca ggtcttcgtg
     3541 cagccggcgt cgggtctcct cggtcgagta gagacccgag gggaagccct tcttcaacgc
     3601 cgagccaggc tcacgtccgc gtgacgcttt gctccagtag ccgcgtgagg aagcgagcgt
     3661 gaacagggat cccaccgtcc tgcgggctcg ctcggcgggg ttgctccaca acggggtgaa
     3721 gatgcggcgc acttcgtctt cctgctctgc ctcacctctg accgcattcg ggtgctctcc
     3781 ctgcgcgtag gcgaagaagt ccgattgggt ccagcctgct gccagatagg agccgatgat
     3841 ggatccggcg ctcgttccga cgatcacatc cgagttggag acatcgatcc cacaatcctc
     3901 gagagccttc aggacccctg cgtgataggc catgccgaca aggccgccac cgcctagtac
     3961 caatcccagc ttcatccagt caccttagtg ctttccgcga aagtagccgc ctaggcatca
     4021 gtctctggtt cctttccccg aaacgcgcat cgcatcactt cgttgatgtc gtcacttgga
     4081 aatgccgccg agaagacttg ttcgtcgccc cggtcctcgt agccgtcggg aagttcgggt
     4141 ttcccccag
//
LOCUS       DATMED010000341         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4120975, whole genome shotgun sequence.
ACCESSION   DATMED010000341 DATMED010000000
VERSION     DATMED010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4120975"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VNP73_08820"
     CDS             <1..505
                     /locus_tag="VNP73_08820"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HWL66061.1"
                     /translation="AIIGPTTAQLHVSSTSPISDLYVQLIDVAPDGTRYYLQRGMLKT
                     SHRAINESLSDKRADGSIYRPFRPHTNPTPVEIDKTYSYLVEVFPVGHILRPGHRLML
                     KLLSPPTVDSYYAYVPKSVPGINTVFHDQTNPSHLTLPIVPLDGVRLGEELAQCALQQ
                     VRCVPAE"
     gene            complement(520..1836)
                     /locus_tag="VNP73_08825"
     CDS             complement(520..1836)
                     /locus_tag="VNP73_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWL66062.1"
                     /translation="MDLATEQGHETVFVNTFTNGILGPDIEMLGPVRDGGHIMINSAP
                     GCWGPMITPAIRGGHEVTQPVTVAGAKVGDAVAIWIKDITVTSLATASGNDQVMEGRF
                     LGDPYVAGQCPDCGELYPETRIEGIGPTSIRCAECGADASPFTFTNGYTISFDDNRRV
                     GVTMSREAAEKIAGDARRYAALPENSKQNPILTFAPHDLVGIATRLRPFMGQLGTTPS
                     TNIPDSHNAGDFGSFLIGAPHEYALTAEELQEHKTDGHLDVDAVRAGAVLICPVKVDG
                     GGIYLGDMHALQGDGEIAGHTCDVSGTVTLKVDVIKNLGIDGPLLFPVPEDLPYLAKP
                     LNEEEKAKALEVARQWDLDELEDSAPISVIGTGPDLNSATENGLKRAADLLDMSVPEV
                     MNRATITGAVEIGRNPGVVQITFRAPTEKLEGEGLLGFAHALYGET"
     gene            complement(1892..>2202)
                     /locus_tag="VNP73_08830"
     CDS             complement(1892..>2202)
                     /locus_tag="VNP73_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66063.1"
                     /translation="RLNSSDPGTLESPGRLQRGEKMVVRVRLGSGLSVLAGRPRLSVE
                     VPENATVTDLISCIERDNPQLAASLGNALAVAEGTQLSRDRLLPDDQEVALLVPAAGG
                     "
BASE COUNT          380 a          734 c          675 g          413 t
ORIGIN      
        1 ggcgatcatc ggccccacca cggcccaact tcacgtttcg tcgacctcac cgatctcgga
       61 tctatatgtg caactgatcg acgtcgcgcc ggacgggact cgctactacc tgcagcgcgg
      121 gatgctcaag acgagccacc gagcaatcaa cgaatcgctt tcggacaagc gagccgacgg
      181 ctcgatctac aggcccttcc ggccgcacac gaacccgacg cccgtcgaga tcgacaagac
      241 gtattcgtac ctggtcgagg tctttcccgt cggccacatc ctgcgacccg gccaccgcct
      301 gatgctcaag ctcctcagcc cgccgaccgt ggacagctac tacgcgtacg tgcccaagag
      361 cgtgccgggg atcaacacgg tgttccacga ccagacgaat ccctcacacc tcacgctgcc
      421 gatcgtgccg ctcgacgggg tgaggctcgg agaagagctc gcgcagtgcg ctcttcagca
      481 ggtcaggtgc gtgcccgccg aatagccaag atctatcgcc tacgtctcac cgtagagagc
      541 gtgtgcgaag ccgagcaagc cctcgccctc gagcttttcg gtcggcgcgc ggaaggtgat
      601 ctgaacgaca cccgggttcc ggccgatctc gaccgctcct gtgatcgtcg cccggttcat
      661 cacctccgga acgctcatgt cgagcaggtc ggcggcgcgc ttcaagccgt tctccgtggc
      721 gctgttcaga tcgggcccgg ttccgatgac ggagatcggt gctgagtcct cgagctcgtc
      781 caaatcccac tgccgtgcga cctcgagcgc cttggccttc tcctcttcgt tcagcggctt
      841 ggcgaggtag ggcaggtcct cgggaacggg gaaaagcagg gggccatcga ttccgagatt
      901 cttgatgacg tcgaccttga gagtcacggt gcccgacaca tcgcaggtgt gacccgcgat
      961 ctctccatct ccctgcaatg cgtgcatgtc gcccagatag atgccgcccc cgtccacctt
     1021 caccgggcag atgagcaccg ctccggcgcg gaccgcatcg acgtcgaggt ggccgtcggt
     1081 cttgtgctcc tgaagctcct cggccgtgag cgcgtactcg tgaggcgccc cgatcagaaa
     1141 ggagccgaag tcgccggcgt tgtgcgagtc ggggatgttc gtcgacggag tcgttcccag
     1201 ttgccccatg aagggacgca gacgagtggc gatcccgacc aggtcgtgtg gagcgaacgt
     1261 caggatggga ttctgcttcg agttttcggg gagtgccgcg tagcggcgag cgtcccccgc
     1321 gatcttctcg gccgcctccc ggctcatcgt cacacccacg cgccggttgt cgtcgaagct
     1381 gatcgtgtag ccgttggtga aagtgaacgg cgacgcgtcg gctccacact ctgcgcagcg
     1441 gatggaggtt ggcccgatgc cctcgatgcg agtctcgggg tagagctcgc cacaatccgg
     1501 acattgaccg gccacatagg gatctccgag gaagcgaccc tccatcacct ggtcgttgcc
     1561 cgatgcggtc gcgagggaag taacggtgat gtcctttatc cagatggcaa cggcgtcgcc
     1621 cactttcgca ccggccacgg tgacgggctg ggtgacctcg tgaccgccgc gaatcgccgg
     1681 tgtgatcatc gggccccagc atccgggcgc gctgttgatc atgatgtggc cgccgtcgcg
     1741 caccggtccg agcatctcga tgtcgggacc gagaatgccg ttggtgaagg tgtttacgaa
     1801 gaccgtttcg tggccctgtt cggtggcgag atccatctcc tcgatggcca cttgccctcc
     1861 tctctccggt gtttccaccg aattggcgcc ctcagccgcc tgccgcgggc acgagcagcg
     1921 caacttcttg gtcgtcggga agcaatcgat cacgtgagag ttgagtgccc tctgccacgg
     1981 ccagcgcgtt gcccaagctc gcggccagct gagggttgtc acgctcgatg cacgagatca
     2041 gatcggtgac ggtcgcgttc tcgggcacct cgacactcaa ccgaggccta ccggcaagca
     2101 ccgacagacc cgagcccaag cggacgcgca ccaccatctt ttccccccgt tgcaatcgtc
     2161 ctggagactc tagcgtgcct gggtcactcg agttcagccg ag
//
LOCUS       DATMED010000342         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2970979, whole genome shotgun sequence.
ACCESSION   DATMED010000342 DATMED010000000
VERSION     DATMED010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970979"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2242
                     /locus_tag="VNP73_08835"
     CDS             <1..2242
                     /locus_tag="VNP73_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWL66064.1"
                     /translation="AWQQIGDPSGDSLVPFSSEFDVAAGNAFVAGPISHPYPSANACR
                     TETTLEGQPGAGTAAACTRGPHADEGLGPVKEGSDEVWGPTGGQTGEENPIPEETDKG
                     LWEELMEAGVPIPDNYDTPSYFALEENTRLHLQAFKMGEVVFASCACEAQVDLILNFE
                     SRVNEVQGDIWDGYDWTAENVLPQTKEVLPPKLLCDQAEPPAGDWSCTPNPDLPRPRA
                     NLPAFTVTDFEYRRMKAQIHNDAAGWDDPENAVAALSEPWDTTQIFGNFTKEELSAEQ
                     GYKLAIGVGHATDYNGYTVSYREFQGYDEYRKALTSYGPHTADYMSTRMVQLAAQLNG
                     FAQPGLEQDIARGLPDEARQLAMSTALGATSLAVYEAWQASFPVDKGPVGKPTAQPKD
                     INVFDAATFKWRGGSNATDNPVVTVERLVDGEWVQFADQSGEIQTKLEFPNGVNAFAD
                     TYAGQHDWIWTANFEAFDALPADIGSTPEGEYRFVVRGTYRSAPATNGKYTVRSLPFT
                     VSAWDGINVGELSLNEDGSVSFDVDPIEYPTSYVSEFPYIKTEGKTIQDDEVIKGGEL
                     GESYCSTCTFRPWASKGEVATATVTVARADGTQTHVTAAKGSDGRWTAPVELFEGDRA
                     FVAQGGVVDNHGEINGQPSNEVQGTRTRPVIQEATQLHLSFVGKGDNRRLMATLKLVD
                     GGERIPSQTIEFFAKGVLIGQDQTNGSGIARLSLSKKLLKPGITFEARFAGNENYLPA
                     SATLSS"
     gene            complement(2243..>2730)
                     /locus_tag="VNP73_08840"
     CDS             complement(2243..>2730)
                     /locus_tag="VNP73_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66065.1"
                     /translation="HGNPILDLISTGLGYLGGMIEALGKPGCTVVLASPCPDRWDEVH
                     HASYPEVWREVLPVTKDPDEARKRFEPEFARRDDYIAKYRHEYSFHPVHAIMALYPLK
                     RLRHAGRVIVAGAEDPAIPRHCGFDYADSVESAIALAKEAHGPDASVALVEYPPAFNR
                     Q"
BASE COUNT          565 a          851 c          854 g          460 t
ORIGIN      
        1 ggcgtggcag cagatcggcg acccgtcggg tgattctctc gttccgttct cgtcggaatt
       61 cgacgtcgct gcaggcaatg ctttcgtggc aggtccgatc tcgcatcctt acccttctgc
      121 caacgcctgc cgcaccgaga ccactctcga aggtcaaccg ggcgcaggta ccgcggcagc
      181 ttgcacccgc ggcccccatg cggacgaagg ccttggaccc gtcaaggaag gctcggacga
      241 ggtttggggc ccgaccgggg ggcagaccgg cgaagaaaac ccgatcccag aggagacgga
      301 caagggattg tgggaggagc tcatggaagc cggagttccg atccccgaca actacgacac
      361 tccttcgtac ttcgcgctag aagagaacac gcgcctccac ctccaggcat tcaagatggg
      421 cgaggtcgtc ttcgcctcgt gcgcctgtga ggctcaggtc gacctgatcc tcaacttcga
      481 gagtcgtgtg aacgaggtcc agggcgacat ctgggacggc tacgactgga cggctgagaa
      541 cgttcttccg cagaccaaag aggttctgcc gccgaagctg ctctgcgatc aggcggagcc
      601 ccctgccggc gattggtcgt gcacgccgaa cccggacctg cctcgacccc gagcgaacct
      661 gcccgccttc accgtgaccg acttcgagta ccgccggatg aaggctcaga tccacaacga
      721 cgccgccggt tgggatgatc cggagaacgc ggtcgcagcg ctcagcgagc cgtgggacac
      781 cacccagatc ttcggcaact tcacgaagga agagctttcc gccgagcagg gttacaagct
      841 tgcgatcggc gtcggacatg cgaccgacta caacggctac acggtcagct accgggagtt
      901 ccagggctac gacgagtacc gcaaggcgct cacgtcgtac ggacctcaca cagccgacta
      961 catgagcact cgcatggttc agcttgccgc gcaattgaac ggtttcgcgc agcccgggct
     1021 cgagcaggac atcgcacggg gcttgcccga tgaagcacgc cagctggcca tgtcgacggc
     1081 tcttggggcg acctccctgg cggtctatga agcgtggcaa gcgtcattcc cagtcgacaa
     1141 ggggccggtc ggaaagccga ccgcgcagcc caaggacatc aacgttttcg atgccgcgac
     1201 gttcaagtgg cgcggggggt cgaacgctac cgacaatccc gtcgtcaccg tcgagcgcct
     1261 ggtcgacggt gaatgggtgc agttcgccga tcaatccggc gagatccaga cgaagcttga
     1321 attccccaac ggggtgaacg cgttcgcaga cacctatgcc ggccagcacg attggatctg
     1381 gacggccaac ttcgaagcat tcgatgctct accggcagac atcggcagca cgccggaggg
     1441 cgagtaccgc ttcgtcgtga gaggcaccta tcgctcggct cctgccacga acggcaagta
     1501 cacggttagg tccttgccct tcaccgttag cgcttgggac gggatcaacg ttggcgaact
     1561 gtcgctgaac gaggacggtt ctgtgagttt cgacgtcgat ccgatcgaat accccacgtc
     1621 ctacgtgtct gagttcccct acatcaagac ggaaggcaag acgatccagg atgacgaagt
     1681 gatcaagggc ggtgagttgg gcgagtctta ctgctcgacc tgcacgttcc gcccgtgggc
     1741 aagcaagggc gaagtcgcga cggccacggt gacggtggct cgagcggacg gcacacaaac
     1801 ccacgtaacg gcggcgaagg gatccgacgg acgctggacc gcgccggtcg agctcttcga
     1861 aggggatcga gcgttcgtcg cacagggcgg tgttgtcgac aaccacggag agatcaacgg
     1921 tcagccgagc aacgaggtac agggaacgag aacaagaccg gtgatccaag aggcaacgca
     1981 gcttcatctg agtttcgtgg gaaagggcga caaccgcaga ctcatggcca cgctcaaact
     2041 tgtcgacgga ggtgaaagaa tccccagcca gacgatcgag ttcttcgcca aaggagtgct
     2101 gatcggccag gaccaaacaa atgggagcgg catcgcgcgg ctgtcactgt caaagaagct
     2161 cctgaagcct gggatcacgt tcgaggcccg tttcgcaggc aacgagaact atctgccggc
     2221 ttccgccacg ctctccagtt agctattgcc ggttgaatgc cggcgggtac tcgaccaacg
     2281 cgaccgacgc gtcgggtccg tgtgcttcct tagcgagagc gatcgcgctc tccactgaat
     2341 cggcatagtc gaaaccacaa tggcgcggga tcgccggatc ctcggccccc gcgacgatca
     2401 cccggcctgc atgccgtaga cgcttaagcg gatacaacgc catgatcgcg tgcaccgggt
     2461 ggaacgagta ctcgtgccgg tacttggcga tgtagtcgtc gcgccgagcg aactcgggtt
     2521 cgaacctctt gcgcgcctcg tccgggtcct tcgtgaccgg aagcacctcg cgccacacct
     2581 cgggatacga cgcatgatgg acctcatccc atctgtcagg acatggggac gcgagcacga
     2641 ccgtgcaccc gggcttacct agtgcttcga tcatcccgcc gaggtagccg aggcccgtcg
     2701 agatcaggtc caggatcggg ttgccgtgcg
//
LOCUS       DATMED010000343         2929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3735537, whole genome shotgun sequence.
ACCESSION   DATMED010000343 DATMED010000000
VERSION     DATMED010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3735537"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VNP73_08845"
     CDS             <1..386
                     /locus_tag="VNP73_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWL66066.1"
                     /translation="FMQEHADPRCRPFDDAFDPLFTFMHDVPRDVAEESGAHVRDQSG
                     TPFEKVWPLDRWPDVPTKVLLGREDRFFPPEFLGPVSRERLGVVPDEMPGGHLVALSR
                     PKELAERLEKYRADLLLDDVFRGSE"
     gene            complement(351..1004)
                     /locus_tag="VNP73_08850"
     CDS             complement(351..1004)
                     /locus_tag="VNP73_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWL66067.1"
                     /translation="MDPKAAYESSFERIRSLVGDQSADVEVPTCPGWTVKDVVAHLAD
                     FFAVFKADPEKGFSRGWGDEGVERRRSRSLQECLQEVSEHLQDPGGFFESQFAAVAVS
                     DVLAHEQDIRTALDRPGARDDANIVPAIEMGLSFIEKKTAGQDLPALRVVTDEVDRTL
                     GEGDASVTLRSSTYELFRTIHGRRTVDQLQAMDWDGDPGPWVSALFLFGPTEDIVEK"
     gene            1097..1717
                     /locus_tag="VNP73_08855"
     CDS             1097..1717
                     /locus_tag="VNP73_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66068.1"
                     /translation="MAISAREGRSAAAFFEELYAIVVGLGLAFAVEQVIDLDRPVLPV
                     RADYLPIFLAYLTLAFALAHSSVRYLQLAYDQRGIVLDKVRVVADLFLGVGQFLLLIA
                     LSLLITRPGVFAFGAIVLLIGRPLRDLVLTLSGHDRLDLDRKVARLHIAVVLILGIAL
                     GAGQLVPARMEDLVLKVATLAASLGFGLGLYIGAFDFFFPRENEAS"
     gene            1788..1952
                     /locus_tag="VNP73_08860"
     CDS             1788..1952
                     /locus_tag="VNP73_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66069.1"
                     /translation="MRKLRLLALSVLIALPIVGGVAAPAHACTSTVEPDGCEVINRVC
                     EKAFKVRCLG"
     gene            1972..2364
                     /locus_tag="VNP73_08865"
     CDS             1972..2364
                     /locus_tag="VNP73_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWL66070.1"
                     /translation="MGESQRIRSGSPYEDKIGFSRALRVGNRVLVSGTAPVWPDGSVD
                     PDPYVQARRCLEIIVLALEEAGAQADQVIRTRAYLTDASYAEEVGRAHSEVFGAARPT
                     STMVVVAGLLDDRWKVEFEAEADLSQEA"
     gene            complement(2366..2731)
                     /locus_tag="VNP73_08870"
     CDS             complement(2366..2731)
                     /locus_tag="VNP73_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWL66071.1"
                     /translation="MVAGIEVGSFDSPSESRTPDKTQVDVVKLGGTTAARITFQPGWK
                     WSECIKPVAGTDSCQARHVGTVVSGRLHVAHDDGSEDEAGPGGVYVIEPGHDAWVVGE
                     EPFVAFEFEAKTAAEFAKE"
BASE COUNT          496 a          964 c          859 g          610 t
ORIGIN      
        1 ccttcatgca agagcatgcc gaccccagat gcagaccgtt cgacgatgct ttcgatcccc
       61 tgttcacgtt catgcacgat gtgccacgcg atgttgctga ggaatcgggt gctcacgtgc
      121 gcgatcagtc gggaacgccg ttcgagaagg tttggcccct cgaccggtgg ccggatgtcc
      181 cgaccaaagt gctactgggc cgtgaggacc ggttctttcc tcccgagttc ctcgggcccg
      241 tgtcgcgcga acgccttggt gtcgttcccg atgagatgcc gggcggtcac ctggtggcgc
      301 tcagccggcc gaaggagctc gccgagcgtc tggaaaagta tcgggccgat ctacttctcg
      361 acgatgtctt ccgtgggtcc gaataagaac aacgccgaca cccacggacc gggatcgccg
      421 tcccagtcca tcgcctgtaa ctgatcaacg gtgcgccgtc cgtggatcgt tctgaagagc
      481 tcgtaggtcg agcttcgcaa tgtcacggaa gcatccccct ctccgagcgt gcgatcgact
      541 tcgtcggtca cgaccctcaa cgcgggaagg tcttgtccgg cggtcttctt ctcgatgaac
      601 gagaggccca tctcgatcgc gggcacgatg tttgcgtcgt ccctggcgcc gggccgatcg
      661 agcgccgtcc tgatgtcttg ctcgtgtgcg agtacatccg aaaccgcgac ggcggcgaac
      721 tgcgattcga aaaagccccc gggatcttgg agatgctcgc tcacctcctg gaggcattcc
      781 tgcagcgatc ggcttcgccg tcgctcgacg ccttcgtctc cccacccgcg tgagaacccc
      841 ttctcgggat cggccttgaa gactgcgaag aagtccgcca gatgtgcgac gacgtccttg
      901 accgtccagc cgggacaggt aggcacctcg acgtcggcac tctggtcacc gaccagcgat
      961 cggatccgtt cgaacgagct ctcgtaagcg gcctttggat ccattccagg ctcccttccg
     1021 gttcctacca aaagtcgtac ccgctggggc cgaagcaaat ccgctccaag tcgtcggaga
     1081 tacatagcgt tgagccatgg ctatctcggc tcgagagggt cgctcggcgg ctgcgttctt
     1141 cgaagagttg tacgcgatcg tggtcggctt ggggctcgct ttcgcggtag agcaggtcat
     1201 cgatctggat cgacccgtgc tgcccgtccg tgcggactac ctccccatct ttctcgctta
     1261 tctgaccctg gctttcgcct tggcccactc gtccgttcgg tacctccagc ttgcttacga
     1321 ccaacgcggg atcgtcctgg acaaagtccg ggtggtggcg gacctcttcc tcggcgtagg
     1381 tcagttctta ctgttgatcg cactctcgtt actgatcact cggccgggcg tcttcgcctt
     1441 cggcgcgatc gtgcttctca tcggacgccc gctccgagac ctcgtactca cattaagtgg
     1501 gcacgatcgg ctggatctcg accgcaaagt cgcccggctt cacatcgccg tcgttcttat
     1561 cctcggtatc gctctaggtg cgggacagct tgtccccgct cggatggagg acctcgtttt
     1621 gaaggttgcg acgctggcgg cctcgctcgg gttcggtctc gggctctata tcggggcctt
     1681 cgatttcttc tttcctaggg agaacgaggc ttcctgaagg aaaacaagcc caaaggtaga
     1741 agcctgccgc atcggctccc ctatcgcggg ctcggaaagg aacggacatg aggaaactgc
     1801 gtcttctggc actaagcgtt ctgatcgctc tcccgatcgt cggcggtgtg gccgccccgg
     1861 cacatgcgtg caccagcacg gtcgagcccg acggatgtga agtgatcaac cgcgtctgcg
     1921 agaaagcgtt caaagtccgc tgcctcggct aggtccccct cggccccttt gatgggtgaa
     1981 agtcagcgga tccgttcggg gtccccatac gaagacaaga tcggcttcag tcgcgcgctg
     2041 cgggtcggta accgtgtcct cgtatccggg accgcgcccg tgtggccgga cggctctgtc
     2101 gatcccgatc cgtacgttca ggcacgccgg tgcctcgaga tcatcgtgct ggccctcgaa
     2161 gaggcaggtg ctcaagcgga ccaggtcatc agaacgcgcg cgtacctaac cgacgcgtcc
     2221 tatgccgaag aagttgggcg tgcacactcc gaggtgttcg gcgccgcgcg cccgacatcg
     2281 acgatggtcg tggtcgcggg gctgctcgac gatcgctgga aggtcgagtt cgaagccgaa
     2341 gcggatctaa gtcaggaggc ttagctcact cctttgcgaa ttcggctgcg gtcttagcct
     2401 cgaactcgaa cgcgacgaag ggttcctcac cgaccaccca cgcgtcgtga ccgggttcga
     2461 tcacgtagac cccgcccggc ccggcctcgt cctcactgcc gtcgtcatgt gcgacgtgga
     2521 gccgtccact caccaccgtt cccacgtgcc tcgcctgaca cgaatcggtt cccgccacag
     2581 gcttgatgca ctcggaccac ttccatcctg gctggaacgt gatccgagcc gccgtggtgc
     2641 ctcccagctt gacgacgtcg acttgcgttt tgtccggcgt gcgcgactca ctgggcgaat
     2701 cgaaactccc cacctcgatg cctgccacca tcaccctccc tgttctcggc ggaacgacgt
     2761 aagcagcgat cttaggcccg catcttcaac cttcacgaag tcgagtcacc attgcgttcg
     2821 acgcgcgctt cagatcctcg ctcgagatct ggaagaccga atcggtccgg ccccccgccg
     2881 cccacacctg atcgagttcc aagagacggt cgtccatagc aacgcgtac
//
LOCUS       DATMED010000344         6494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_290316, whole genome shotgun sequence.
ACCESSION   DATMED010000344 DATMED010000000
VERSION     DATMED010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290316"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VNP73_08875"
     CDS             complement(<1..780)
                     /locus_tag="VNP73_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWL66072.1"
                     /translation="MDETIVAFLKALTAERDYSAHTVSAYERDLEQFAEWSGRGKIHG
                     IKEVDRRHLRRYLGYLTQRGYARRTIARKLSALRSLFKWALLHSLITIDPTTDLDSPK
                     LDRPLPRVLKPAEAVRLVEAPPADDPVGARDRAVLEILYGSGLRVAELCSLDIDDLDL
                     RGGALNVTGKGRKQRRVPLGDAARSALARYLEEARSTFDPKDAAAVFLNSRGTRLGPR
                     TVRSVIARYTLADGLPPTSPHGLRHSYATHLLDGGADLRAVQ"
     gene            complement(916..1824)
                     /gene="dprA"
                     /locus_tag="VNP73_08880"
     CDS             complement(916..1824)
                     /gene="dprA"
                     /locus_tag="VNP73_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWL66073.1"
                     /translation="MSGSARVIRAGDPEWPFHLTQIPRPPDRLWLEGCSLRSGRDAVA
                     VVGTRRPTAAGLEAARDIARGLAQAGFVVVSGLAVGIDTAAHKAAIEAGGETVAVLGC
                     GLDHMYPRVNTKLRKDITSCGTVASEFTPDTPPRAAHFPQRNRIIAGLSSAVVVIEGS
                     LRSGARITASFALDFDRDVFAVPGSIRNPMAAAPNELIRTSQATLVTDIKHITDELAP
                     GLVWDGGLGREEAAVALDDHEKAVLSLLDDVPVSPDFVGSETRLPPGRVAVALSRLEV
                     RNLARRRPGGYELTEAGGRARSTLFS"
     gene            complement(1821..3353)
                     /locus_tag="VNP73_08885"
     CDS             complement(1821..3353)
                     /locus_tag="VNP73_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWL66074.1"
                     /translation="MLAKTGSVAFVGVEARLVEVEVDVNTGVPKFTIVGLPAKSVREA
                     EQRTRSALLASQERWPPARVVANLAPAGLRKDGTHFDLPIALGLIAADYGRRLDPNSL
                     ADWVVIGELGLDGSVRRVRGALAAALACKRFGRRGLICPAANHAEASVVEGIEVIPVS
                     CLAEACAFLRGDTAPPAVTERRPVSVPSYADLSDVRGQGGAKTAVEIAAAGGHNVLFY
                     GPPGSGKTMLARRVPGILPEMSLAESLEASQIHSVVGLLPEGIALLAHRPFRAPHHNI
                     SLSGLIGGGTGLAVPGEVSLAHHGVLFLDEMPLFRRDSLEGLRGPTEEGIVHISRVGG
                     AVSYPARFSLVAAMNPCPCGYLDDRKTPCRCSDLDVDRYKSRLSGPLLDRFDVQIIMS
                     RPTRKELLDDPPGESSADVRIRVQAARAIQTERYGTSLRTNATATKAELDSPELLTTA
                     ARNELKSIIDRVELTGRGVIRILRVARTIADLGAHDRVTEDDILNSLTFRLSENLLGA
                     AA"
     gene            complement(3435..4223)
                     /locus_tag="VNP73_08890"
     CDS             complement(3435..4223)
                     /locus_tag="VNP73_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWL66075.1"
                     /translation="MSDYRSLKWEIDSNVATITLDRPEKKNAMSWVMFNEIRAVFDRA
                     AQDPEVRCVVLTGAGGAFCSGADLTDPENMVGGTFEFKDRMKTIDGIVRAFIECPKPT
                     IAKVTGIAAGAGCNLALGCDLIVATREAAFAELFVKRGLVVDFGGTWALARSLSLPRA
                     KEVALLGDTISAEQASEWGMINRLCEADEIDAVVKDLATRLAAMPPRTVSMIKENLNR
                     AVERSLEENLDAESAGQTLAFGSEDTREAITAWIEKREPRFTGR"
     gene            complement(4234..4587)
                     /locus_tag="VNP73_08895"
     CDS             complement(4234..4587)
                     /locus_tag="VNP73_08895"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWL66076.1"
                     /translation="MHARTVFGHAVEDIAADHLEQLGFRIVARNYRCDAGEIDIVALG
                     EGVLVFCEVKARRDDRFGSPAEAVTYAKQARLRRLAACWLAEHRPGAVDIRFDVVSVI
                     VRSGRVELTHIPDAF"
     gene            complement(4622..4945)
                     /locus_tag="VNP73_08900"
     CDS             complement(4622..4945)
                     /locus_tag="VNP73_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HWL66077.1"
                     /translation="MSVEDLEKYEAEMELQLYREYRDVLPMFKYVVETERRFYLANTV
                     NVQTQHAGAKVWFEVELGDAWVWDMHRPARFVSHVKVVTFRDLNVEELAQLPEVPEPP
                     GVERT"
     gene            complement(4942..5631)
                     /locus_tag="VNP73_08905"
     CDS             complement(4942..5631)
                     /locus_tag="VNP73_08905"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWL66078.1"
                     /translation="MTGASIGRMEERLAAAGFEHVAGVDEVGRGALAGPLVAAAVVLP
                     PSIELEGLRDSKACTEKQRDRLAAEIQEVALGMSVARITHDRIDRVGLQRCNRDAFRR
                     ALRNLPLDPDYVLVDFLRMKRFSLRPWPGLAVKKGDAVSKNVAAASIVAKVFRDRIMR
                     RYHRRYKQYGFATNVGYGTREHWDALMRFGPSPIHRRSFYGVEGFVDERGVFSPHPAR
                     GCEEIDLEASR"
     gene            complement(5628..6362)
                     /gene="lepB"
                     /locus_tag="VNP73_08910"
     CDS             complement(5628..6362)
                     /gene="lepB"
                     /locus_tag="VNP73_08910"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWL66079.1"
                     /translation="MQAESETEPVAPILMPDPEEEKRARRKARMRRWGRAILEFPFLV
                     LIAFVIAIVIKTFLVQAFYIPSGSMRPTLEVQDRVLVEKVSYRLRDPEREDIVVFAKE
                     VFGITPPDVPWSQDFRNFLRELAGLPTGTEEDYIKRIVAIAGDTIRYVGRPRRLFING
                     SAVPNPYIRGGRDSTSSSLTSADCARLDMEEADSGCRVPAGMVFVMGDNRPNSEDSRI
                     LGPIKEEKIIGRAFVVIWPANRLGLL"
     gene            complement(6372..>6494)
                     /locus_tag="VNP73_08915"
     CDS             complement(6372..>6494)
                     /locus_tag="VNP73_08915"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HWL66080.1"
                     /translation="VLGDNRGNSDDSRSFGAVPEDRIVGHAFVLIWPPADLSTL"
BASE COUNT         1237 a         2123 c         1953 g         1181 t
ORIGIN      
        1 ctgcaccgcc cgtaggtcgg ccccgccatc cagtaggtgc gttgcgtagg agtggcgcag
       61 gccgtgggga ctcgtgggtg gcagaccgtc ggcgagggtg tatctggcta tcaccgaccg
      121 caccgtccgg gggccgagac gcgtcccgcg cgaattcagg aacacggcgg ccgcgtcctt
      181 gggatcgaag gtcgatcggg cttcctccag gtatctcgcg agagcagagc gagcggcgtc
      241 accaagagga acgcgtcttt gcttccgtcc cttcccggtg acgttcagcg cgccccccct
      301 cagatcgagg tcgtcgatgt ccagggagca gagctcggct actcgcaggc cgctcccgta
      361 gaggatctca agcaccgccc ggtcgcgagc gccgaccggg tcgtccgccg ggggggcctc
      421 gaccaggcgt acggcctcgg caggtttcag gacgcgtggg agcggacggt ccagcttggg
      481 agaatcgagg tccgtcgtcg ggtcgatcgt gatcagcgag tgcaaaagag cccatttgaa
      541 gagtgatctc aaggcgctga gctttcgcgc gatcgtccgg cgcgcgtatc cccgttgggt
      601 cagatatccg agatagcgac gaaggtgtcg ccggtcgacc tccttgatcc cgtggatctt
      661 cccgcgaccg gaccactcgg cgaattgttc gagatcgcgc tcgtaggcgc tgaccgtatg
      721 cgccgagtag tcccgctcag ctgtgagggc tttgagaaag gcgacgatcg tctcgtccac
      781 ctgtaaagga tggccgcgcc caggttggga gtcaacgatt gctatttggt acccagtggt
      841 ttcactggtt cgactggttc atgtggtcgc gtaaaaacca gcagaaccag tgtgaccact
      901 tgtttctcag atcctctacg agaacagcgt gctcctcgcc cgaccccccg cctcggtcag
      961 ctcgtaacca cccggccgcc tacgcgcaag attgcgaact tccaatctgg agagcgccac
     1021 cgcgacgcgt cccgggggaa gtcgtgtttc gctgccgacg aaatctgggc tgaccggaac
     1081 gtcatccagc agcgagagca ccgccttctc gtgatcgtcg agagcaactg cagcctcttc
     1141 ccgacccagc cccccatccc acacaagtcc gggcgccaat tcatccgtga tgtgtttgat
     1201 atccgtcaca agggttgcct gggacgttcg gatcaattcg ttcggtgctg cagccatcgg
     1261 gttccggatg ctgcccggaa ccgcaaacac gtcgcgatcg aagtcaagtg caaaagatgc
     1321 tgtgatcctg gcaccgctcc tcaagcttcc ttcgatcacc acgacggccg acgacagtcc
     1381 ggcaattatc cggttccttt gcggaaaatg agctgcacga ggcggagtgt ctggtgtgaa
     1441 ctccgaggcg accgttccgc atgatgtgat gtctttgcga agcttggtgt tcacacgtgg
     1501 atacatgtgg tccaaaccgc agccgagaac agcaaccgtt tcgcccccgg cttcgatcgc
     1561 cgccttgtgt gctgcggtgt ctatccccac agcaagcccc gatacaacga cgaatccagc
     1621 ctgtgcaagt cctcttgcga tgtcccttgc cgcctcaagg ccggctgccg tcggccttct
     1681 cgtcccgacc acggcaactg cgtcacgacc cgatcgaagg gaacatccct ccaaccaaag
     1741 tcggtccggt ggtcgcggga tctgcgtcag gtgaaaaggc cattccggat cgcccgccct
     1801 gatgactctc gcagatccgc tcatgcggcg gctcccaaca ggttctccga caatcgaaac
     1861 gtcagtgaat tcaagatgtc gtcctcggtg acacgatcgt gcgctcctag atccgcgatg
     1921 gttcgtgcca cccgaaggat ccggatcact ccacgaccgg ttagctcgac ccggtcgatg
     1981 atcgacttca actcgttccg cgcggccgtt gtcagaagtt ctgggctatc gagttctgct
     2041 ttcgttgctg ttgcattcgt ccttagcgaa gtcccgtagc gctccgtctg gatcgctcgg
     2101 gcagcttgca cccgaatacg tacatcagca gacgattcgc ctggggggtc atccaaaagc
     2161 tccttccgtg taggccgaga catgatgatc tgcacgtcga acctgtcgag gagaggcccc
     2221 gaaagtcgcg acttgtatcg gtcgacatcc agatcgctgc agcggcacgg cgtctttcga
     2281 tcgtccagat atccgcacgg gcacgggttc atcgccgcga cgagcgagaa gcgcgcgggg
     2341 taactcaccg cgccaccaac tcgggagata tggacgatcc cctcctccgt ggggccgcgc
     2401 aaaccttcga gcgaatctcg gcggaagagt ggcatctcgt ccaagaagag gacgccatgg
     2461 tgagccaggc tgacttcacc cggaaccgcc aaaccggtgc caccgccgat caaccccgag
     2521 agggagatgt tgtgatgagg agctcgaaac ggccggtgag caagcagggc tatgccttct
     2581 ggcagtaacc ccaccacgga gtggatctga ctcgcctcga gtgattcggc gagcgacatc
     2641 tccggaagga tccccggaac gcgacgagcc aacatcgtct tacctgagcc cggcgggccg
     2701 tagaacaaga cgttgtggcc gcccgcagcc gcgatctcaa cagcagtctt cgcgcctcct
     2761 tgaccccgaa cgtccgagag atcggcgtac gaaggcactg aaacgggtct tcgttccgtg
     2821 acggccggcg gtgcggtatc accgcgcagg aaggcacatg cttcagccag gcaggagacg
     2881 gggatcacct caatcccctc gacaacagaa gcttcagcat gattggcggc cggacagatc
     2941 agacctctcc tgccgaagcg tttgcaagca agagcggcag cgagggcgcc acgaacgcgt
     3001 cggactgagc catcgagacc caattcacct atgacgaccc agtcggccag agagtttggg
     3061 tccagtcgcc ggccgtagtc cgcggcgatc agacccaggg ctatcggcag atcgaagtgc
     3121 gttccgtctt ttcgaagccc ggcgggggca aggttcgcaa cgacgcgcgc agggggccag
     3181 cgttcctgcg aagccaacag agccgagcgc gtgcgttgtt cggcctccct caccgacttt
     3241 gcgggtaatc caacgatcgt gaacttgggc actcccgtgt tgacgtcgac ctcgacctcg
     3301 acgagtcggg cttcgacgcc cacaaaggcg acagacccgg tcttggccag catggaacct
     3361 ccgatccggg ggccgggata cgcgcggagg tcagaaggta acgatggggt gcgacagaaa
     3421 gagtggcgcg ggcctcagcg gccggtgaag cggggctctc tcttctctat ccacgcggtg
     3481 atcgcctcac gggtgtcttc ggatccgaaa gcgagcgtct ggccggccga ttcggcatcg
     3541 aggttctcct ccagcgatct ctccaccgcc cggttcaggt tctccttgat catcgacacg
     3601 gtcctcggcg gcatcgccgc caaccgcgtc gccagatcct tgaccacggc atcgatctca
     3661 tccgcctcgc aaaggcggtt gatcatcccc cactcgctcg cctgctcggc cgagatcgtg
     3721 tcccccagca atgccacttc cttggcacgc ggcaacgaca aggacctcgc cagagcccac
     3781 gtcccgccaa agtccacgac tagccctcgc ttcacgaaga gctctgcaaa agccgcctcc
     3841 cgcgtcgcca cgatcaggtc gcatcccaat gcgagattgc atccggcccc cgctgcgatc
     3901 cccgtaacct tcgcgatcgt gggcttcgga cattcgatga aggcacgcac gatcccgtcg
     3961 atcgtcttca tccggtcctt gaactcgaac gtgccgccga ccatgttctc gggatcggtc
     4021 aggtccgccc cggaacagaa cgcgccgccg gcgccagtca gcacaacgca acgaacctct
     4081 ggatcctgcg cggcccgatc gaagacggcg cggatctcgt tgaacatgac ccacgacatg
     4141 gcgttcttct tctcgggtcg atccaacgtg atcgtcgcga cgttcgaatc aatctcccac
     4201 ttgagggatc gatagtccga caacggaacc tcctcagaag gcatcgggga tgtgagtcag
     4261 ctcgacgcga ccggaacgaa cgataacgga gacaacatcg aaacggatgt ccacggcgcc
     4321 gggcctgtgc tcggcgagcc agcacgcggc cagccgccga aggcgggcct gcttggcgta
     4381 ggtgacggcc tcggcgggcg aaccgaaccg gtcgtcgcgt ctggccttga cctcgcagaa
     4441 gacgagaacg ccctctccga gcgcaacgat gtcgatctca ccggcatcgc agcgatagtt
     4501 gcgagccacg atccggaagc cgagttgctc gagatgatcg gctgctatgt cctcaacggc
     4561 gtgcccgaaa accgtgcgcg catgcatcgg ccgaccttaa ggagggggtg cgacagaaac
     4621 tctaggtgcg ttccactccc ggcggctcgg ggacctcggg caactgagcc agctcctcga
     4681 cgttgagatc ccggaaggtc actaccttca cgtgagacac gaagcgagcc ggcctatgca
     4741 tgtcccacac ccatgcatca cccagctcga cctcgaacca cactttggcc cccgcgtgct
     4801 gcgtctgaac gttcacggtg ttggcaagat agaagcggcg ttccgtctcg acgacgtact
     4861 tgaacatcgg gagtacatcc cggtactccc ggtagagctg gagctccatc tcggcttcgt
     4921 acttctcgag atcctcgacg ctcaccggct cgcctccagg tcgatttcct cacatcctcg
     4981 cgccgggtgg gggctaaaga cacccctttc gtccacgaat ccctcgaccc cgtagaagga
     5041 tcttcgatgg atcggcgagg gcccgaatct catcaaagca tcccaatgct ccctcgtccc
     5101 gtatcccaca ttcgtggcga acccgtattg cttgtagcgg cggtggtagc gcctcatgat
     5161 ccggtccctg aacaccttgg ccacgatcga tgcggcggcc acgttcttgg atacggcgtc
     5221 ccccttcttg accgccaggc cgggccacgg ccgcaaggag aacctcttca tgcgtaggaa
     5281 atcgaccaac acgtagtcgg gatccagagg gaggttcctc agcgccctgc ggaaggcatc
     5341 ccggttgcat ctctggaggc cgacccgatc gatgcgatcg tgcgtgatcc gggcgacaga
     5401 catccccagg gccacctcct ggatctctgc cgcgagacga tcacgctgct tctcggtgca
     5461 cgccttcgag tccctgagtc cttcgagctc gatcgaagga ggcaggacga cggcggccgc
     5521 taccagcggc cccgccagtg cgccgcggcc gacctcgtcg acaccggcta cgtgctcgaa
     5581 gcccgcggcc gcgagccgct cctccatccg tccgatggag gcaccagtca cagcagtccc
     5641 agcctgttcg ccggccagat gacgacgaag gcccggccga tgatcttctc ttccttgatc
     5701 gggcccagga tccgggagtc ctccgagttc ggccggttgt ctcccatgac gaagaccatc
     5761 ccggccggaa cgcggcaccc cgagtctgct tcttccatgt cgaggcgcgc gcagtccgcc
     5821 gaggtcaaag acgacgacgt cgagtctctg ccccctctta tgtaggggtt aggtaccgcc
     5881 gacccgttga tgaagagccg gcgcggacgg ccaacgtagc ggatcgtgtc cccggcgatc
     5941 gcgacgatcc gcttgatgta gtcctcttca gtacccgtcg gcagaccggc gagttcgcgc
     6001 aggaagttac ggaagtcctg cgaccaggga acgtcggggg gcgttatgcc gaagacctcc
     6061 ttggcgaaca ccacgatgtc ctcgcgctcg gggtcgcgca accggtagga caccttctct
     6121 acgagaaccc ggtcttgaac ctcgagggtg ggtcgcatcg aacccgaggg gatgtagaag
     6181 gcttggacga gaaaggtctt gatgacgatc gcgatcacga acgcgatcag gaccaagaag
     6241 gggaactcca agatggctcg tccccatctc ctcatccggg ccttgcggcg ggcgcgcttc
     6301 tcctcctctg gatcgggcat gagtatgggc gcgaccggct cggtctcgga ttccgcctgc
     6361 atgcgaacag actagagggt ggacagatct gcgggcggcc atatcagaac gaaggcatgg
     6421 cccacgatcc tgtcttctgg gacggcgccg aaacttcgcg agtcgtcgga gttgccacgg
     6481 ttgtcgccga gaac
//
LOCUS       DATMED010000345         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2596600, whole genome shotgun sequence.
ACCESSION   DATMED010000345 DATMED010000000
VERSION     DATMED010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596600"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..728
                     /locus_tag="VNP73_08920"
     CDS             36..728
                     /locus_tag="VNP73_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL66081.1"
                     /translation="MEGAVANQSKEDAIRVLIVDDHALFRRGLQMVLEGEKDIEVVGE
                     AEDGHAAVEKAEHSTPDVVLMDVRMPKRTGIEATRSIKDTLPSTKILMLTISDEEADL
                     YEAIKAGASGYLLKEISIEEVADAIRAVHAGQSLISPSMASKLLNEFATMVKRRDERA
                     QVPGPRLTERELEVLKLVAKGMNNRDIGTELFISENTVKNHVRNILEKLHLHSRMEAV
                     VYAVREKLLDIK"
     gene            784..1326
                     /locus_tag="VNP73_08925"
     CDS             784..1326
                     /locus_tag="VNP73_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL66082.1"
                     /translation="MILQTERLILEPWSVDRIEDLVRLSSDPAVMRFIGSGDPWERER
                     ADHMHSDLLNHWSEHGFGWRTIRQADESEVIGVLGLSYTGPEAVEIPTPEVEIGWWLD
                     PEVWGRGLATEAAARVRNDAFERLRLDKVIARHQVANPTSGRIMEKIGMTFEREAVGR
                     HGDHVRIWAIGRDDWLRLGR"
     gene            1374..>2180
                     /locus_tag="VNP73_08930"
     CDS             1374..>2180
                     /locus_tag="VNP73_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWL66083.1"
                     /translation="MSIIKKVLAAGESKKIKALQAIVPQVNSFAEEYKALSDDALRGK
                     TPELKQRLENGESIDSLLPEAFATIREGADRAIGQRHFDVQLMGGAALHRGWIAEMKT
                     GEGKTLTGTLAVYLNALWEEGVHVVTVNDYLAKRDSEWMGQIYKFLGMSVGLIQAQMR
                     PTDRRPAYAADVTYGTNNEFGFDYLRDNMVTERENMSQRGHFYAIVDEVDSILVDEAR
                     TPLIISGAIQESTKWYQQFARISPRLHPDEHYEVDEKKRTVAISEEGVTQV"
BASE COUNT          486 a          638 c          682 g          374 t
ORIGIN      
        1 ttagaatcaa tcggactagt gcagcaggag tagtcctgga gggggccgtg gccaatcaga
       61 gcaaagagga tgccatccgc gtccttatcg tcgacgacca cgctctattc cgtcgcggac
      121 tccagatggt cctcgaaggc gagaaggaca tcgaggtggt gggcgaggcc gaagacggtc
      181 atgccgcggt cgagaaggcc gagcacagca ccccggatgt ggtcctaatg gacgtccgga
      241 tgcccaagcg gacgggcatt gaggcgaccc gctctatcaa agacacgctc ccttccacga
      301 agatcctgat gctgacgatc tctgatgaag aggccgatct ctacgaagcg atcaaggccg
      361 gtgcctcggg ctatctcctg aaggagatct cgatcgaaga ggttgccgac gcgatccgag
      421 ccgtacacgc gggtcagtca ctgatctcgc cgtcgatggc gtccaagctc ctgaacgagt
      481 tcgcaacgat ggtcaagcgc agggacgagc gtgcccaggt acccggcccc cgtctcaccg
      541 aacgcgagct cgaggtgctg aagctcgtgg ccaagggtat gaacaaccgg gatatcggca
      601 cggagctgtt catctccgaa aacacggtca agaatcacgt gcgcaacatc ctcgagaagc
      661 tccatcttca ctcccgcatg gaggccgtgg tctacgcggt ccgcgagaag ctcctagaca
      721 tcaaataaca gttctttctt aacatttgcc cccaccccag gtgggggcgg ttagaaaaga
      781 acagtgatcc ttcaaaccga gcgcctgatc ctcgagccct ggtcggttga ccggatcgaa
      841 gacctggtcc gtctttcctc cgaccccgcc gtgatgcgtt tcattgggtc cggagatcca
      901 tgggagcggg agagggccga ccacatgcat tcggatctcc tcaatcactg gtccgaacac
      961 ggattcggct ggcgcaccat caggcaagct gatgagtccg aggtgatcgg ggtcctcgga
     1021 ttgagctata ccggtcccga ggccgtcgag ataccaactc cagaggtcga gatcggttgg
     1081 tggctcgacc cggaagtttg gggacgaggt ctcgcgaccg aagccgcggc gagggtgcgc
     1141 aacgatgcat tcgaacgtct tcgcctcgac aaggtcatcg cacgacacca ggttgccaat
     1201 cccacttcgg gcagaatcat ggagaagatc gggatgacgt tcgagaggga ggcggtggga
     1261 cggcacggcg atcacgttcg gatctgggct atcggacgcg atgactggct tcgtcttggc
     1321 aggtagacag gcaaaaccgg atactgcgac cggccggaga taatggagcg tctatgagca
     1381 tcatcaagaa ggtcctcgcc gccggtgaga gcaagaagat aaaggccctc caggccatcg
     1441 ttccgcaggt caactcgttc gccgaggagt acaaggcgct ttcggacgat gcgctccggg
     1501 gcaagacgcc ggagctcaag cagcgcctcg agaacggcga atcgatcgac tcactgttgc
     1561 cggaagcctt cgccacgatc cgagagggag ccgaccgggc gatcggccag cgccacttcg
     1621 acgtccagtt gatgggcggt gcggcgcttc accggggctg gatcgccgag atgaagacgg
     1681 gtgaaggaaa gaccctcacc ggaactctcg ccgtctactt gaacgcgctg tgggaagagg
     1741 gcgttcacgt cgtcaccgtc aacgactact tggcgaaacg tgactccgag tggatgggcc
     1801 agatctacaa gttcctcggg atgagtgtcg gattgatcca ggcccagatg agacccaccg
     1861 acagacgacc ggcctacgcc gccgacgtta cctacgggac caataacgag ttcggattcg
     1921 actacctccg cgacaacatg gtgaccgagc gagagaacat gtcgcagcgc ggccacttct
     1981 acgcgatcgt cgacgaggtc gactcgatcc tcgtcgacga ggcgagaacc ccgctgatca
     2041 tctcgggtgc gatccaggaa tcgaccaagt ggtaccagca gttcgcacgg ataagcccgc
     2101 gcctccaccc cgacgagcac tacgaggtcg acgagaagaa gcgtacggtc gcgatctcgg
     2161 aggagggtgt gacccaggtc
//
LOCUS       DATMED010000346         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1031381, whole genome shotgun sequence.
ACCESSION   DATMED010000346 DATMED010000000
VERSION     DATMED010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031381"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..514)
                     /locus_tag="VNP73_08935"
     tRNA            complement(432..514)
                     /locus_tag="VNP73_08935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(479..481),aa:Leu,seq:cag)
     gene            610..1383
                     /locus_tag="VNP73_08940"
     CDS             610..1383
                     /locus_tag="VNP73_08940"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HWL66084.1"
                     /translation="MGLARSIEERLEGLVEGFFTKVFRSGLQPIEVGRRILREMGETK
                     TVSVNRIYAPNEFRVRMSQDDHSRFSQMEAGLQREFSDLVIEQAKQNRWNLMGLPRIS
                     FVKDESLGKGEFKVEASLTADPDSHELKVSTREPIEGRERPRAVSSDTADRIGAMASA
                     ELIVLDDKGDPLERIAITRDPIVIGRMSTSDVVLADSNVSRRHAELKRDGDGWLLVDL
                     GSTNGTLVNGKVAREHRLKDGDRITFGTSELAFKLTDRT"
     gene            1383..1829
                     /locus_tag="VNP73_08945"
     CDS             1383..1829
                     /locus_tag="VNP73_08945"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWL66085.1"
                     /translation="MPDIVLVLLKYAFLAVLYIFIARVVKAVYVELRPGATATTAAAP
                     RSSARRAKKAPRKAAVIEGNPKGKVLELGDELVIGRAEKCHLVIDDTYVSQLHARIFG
                     RGDSFVVEDLGSTNGTYVNRRRITAPTELQRGDRVKIGKTVLEMRK"
     gene            1826..2602
                     /locus_tag="VNP73_08950"
     CDS             1826..2602
                     /locus_tag="VNP73_08950"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWL66086.1"
                     /translation="MSGQVMPRVAVRSDTGRVRDLNEDSYLTQEPLFVIADGMGGHLA
                     GDVASQTAIEIITANRGDASSDLDSLERLVLKANEAIWGKAQADPSMHGMGTTLTLVF
                     LDGSKGHLAHVGDSRAYLFRDGKLEQVTEDHTLVERMVKEGRLPREEAARHPQRNIIT
                     RALGMDADVKVDTFELALRAGDRILLCSDGLNSMLSDEEVEEVLNTFPDADEATDRLI
                     EMANEAGGEDNITVILLDMVEGSTASAGVRERPPSPKRLR"
BASE COUNT          533 a          800 c          852 g          431 t
ORIGIN      
        1 ctgagggccc ccgaggaccg tcaactcgag ggtgtcgggg ttggtggcca cggccttatc
       61 gctctcggca tcgaaggtcg cctcccacgc cagatctcgg cgattgcgcc tcaccaggcc
      121 ccagacggca gaggcaatca acagaccctc cacacccgct ccccgcgtcg ccccgcatcg
      181 acagatcgcc cgctacccca cgatccatcc tcaggggcaa tacgagcttc gcaatggccc
      241 cagctagcta taaagcgcgt agcccggaga aacaattcaa gtgggcgagg ggggtggggg
      301 ggtctggggg gctggtagcg agcgctcgcg cgagcggacc cccccaggtt ccggagagcg
      361 aggactgctt cagcagaact cgctcggagg aacaatttgg gttaggcgtc cctcgaccaa
      421 cggcgaagcc gtgggcgagg ggggatttga acccccacac tcttgcgagc actggcacct
      481 gaagccagcg tgtctgccgt tccaccactc gcccggggtg cggagtaccg caggatatag
      541 aacgcccgct cggtaggtag tcatccctct tgggcataat ccgggcacac tctccccgtt
      601 aggaggccag tgggactcgc acgaagtatc gaagaacggc tcgagggact ggtcgaaggg
      661 ttcttcacga aggtcttccg ttcgggcctt cagccgatcg aggtcgggcg tcgcatcttg
      721 cgcgagatgg gcgagaccaa gaccgtgtcg gtcaaccgaa tctatgcgcc gaacgagttc
      781 cgggttcgta tgagtcagga cgatcactcc cggttcagcc agatggaggc cggcctccag
      841 cgcgagttct ccgatctcgt catcgaacaa gccaagcaga accggtggaa cctgatgggg
      901 ctgccgcgga tctctttcgt gaaggacgag tccctcggca aaggcgagtt caaggtcgag
      961 gcgtccctca cggccgatcc ggattcgcac gagctgaagg tctcgacccg cgagccgatc
     1021 gagggccgcg agcggcctcg ggccgtgtcc tcggataccg ccgaccggat cggtgcgatg
     1081 gcgtcggcag agctgatcgt tcttgacgac aagggcgacc cgctcgaacg catcgccatc
     1141 acgcgggacc ccatcgtgat cggccggatg tccacgagtg atgtcgtgct tgccgactcg
     1201 aacgtcagcc ggcgtcacgc cgagctcaag cgcgatggcg acggctggct gctcgtcgat
     1261 ctcgggtcga cgaacggaac cctcgtgaac ggcaaagtgg cccgggagca tcgcttgaag
     1321 gacggggaca ggatcacgtt cggtaccagc gaactcgcct tcaagctgac ggatcgaacc
     1381 tgatgccgga catcgttctc gtccttctca agtacgcgtt ccttgcagtc ctgtacatct
     1441 tcatcgctcg cgtggtcaaa gccgtttacg tagagcttcg gccgggcgcc acggcgacca
     1501 ccgcagcagc accgcgatcg tcggcgcgcc gcgccaagaa agctccccgt aaggcagcgg
     1561 tgatcgaggg gaaccccaaa gggaaggtgc tcgagctcgg cgacgagtta gtcatcggac
     1621 gcgccgagaa gtgtcatctc gtcatcgacg acacctacgt ttcgcaactg cacgcacgga
     1681 tcttcggcag gggtgattcg ttcgtagtcg aagaccttgg atccacgaac ggtacctacg
     1741 tgaatcgtcg ccggatcacg gcaccgaccg agcttcaacg cggtgaccgc gtcaagatcg
     1801 gcaagaccgt tctggagatg cgcaaatgag tggacaagtg atgccgcgcg tcgcagttcg
     1861 aagcgatacg ggcagggtcc gggatctgaa tgaagactcc tacctgacgc aagaacctct
     1921 gttcgtcatc gccgacggga tgggagggca tctcgccgga gacgtcgctt cgcaaaccgc
     1981 gatcgagatc atcacggcga atcgcggcga tgcgtcgagc gacctcgaca gcctggaacg
     2041 gctagtgctc aaggcgaacg aggcgatctg gggcaaggcg caggccgatc ccagcatgca
     2101 cggtatgggg acgacgctga ccctcgtctt cctcgatgga agcaagggtc acctcgctca
     2161 cgtaggcgat tcaagggcgt acttgttccg agacggcaag ctcgaacagg tgaccgagga
     2221 ccacacattg gtggaacgga tggtcaagga aggtcggctc ccgcgggagg aagccgcgcg
     2281 tcacccgcaa cgaaacatca tcacgcgggc ccttgggatg gatgccgacg tcaaggtaga
     2341 tacgttcgag ctcgccctcc gagccggaga ccgcatcctg ttgtgcagcg acggactcaa
     2401 ttcgatgttg tccgacgaag aggtcgaaga ggtcctgaat accttccccg atgccgacga
     2461 agccaccgac cgtctgatcg agatggcgaa cgaggccgga ggtgaagaca acatcacggt
     2521 gatcctgctc gacatggtcg agggatcgac cgcgagcgcg ggggtgcggg agcggccgcc
     2581 gtcgccgaag cgactccgat agaggggtcg gaggcg
//
LOCUS       DATMED010000347         4298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2975182, whole genome shotgun sequence.
ACCESSION   DATMED010000347 DATMED010000000
VERSION     DATMED010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975182"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /gene="thyX"
                     /locus_tag="VNP73_08955"
     CDS             <1..628
                     /gene="thyX"
                     /locus_tag="VNP73_08955"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWL66087.1"
                     /translation="EFANRFASHLGYAKDLSEIPSRRGVKKLAHPVKVISVEYVGLEQ
                     TYDLCVEGPWHNFVANGFVVHNSFNEESARYHKLSDDFYVPDVPAVRSQVGKPGSYSF
                     EPVDEALANETRDTFQRIYKELYVEYNELIEKGVAKEVARSILPVGIFTQFYWTLNAR
                     ALMNFLSLRNAQTAQYEIRMYADAVERLFAQQMPITHQCFIEFGRVAP"
     gene            618..1667
                     /locus_tag="VNP73_08960"
     CDS             618..1667
                     /locus_tag="VNP73_08960"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HWL66088.1"
                     /translation="MRPDVILSAAAFKFGIDPLAWKIVPALELGPLSISPHGIGIAVG
                     FLLGAQLMVKRATKDTWPVENDIWNTLFWALIGAIVGARVGYVIGHLPEVTSNGDDIL
                     GVLRVWEGGISLIGGITGAILFALPYMIRHKMGFWRTMDLAAPGLALGIIIGRIGDLI
                     IGDHLGKPTDIALGWRCLGTIQGDEPLVAERYLAAARLGSHPSLGCFDVALHQTALYD
                     FFSTILLLGVLLALGRAARRTGFLILVFTVWYGAMRVFTDFLRVDRRYLGLTGSQLMS
                     LAVGLACLYLLARYRGAPPGWIRPLAPGPEGGPPAGDREPLVPAGAPPAASEKLPIAE
                     SEQETPILGADDDSP"
     gene            1781..1936
                     /locus_tag="VNP73_08965"
     CDS             1781..1936
                     /locus_tag="VNP73_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HWL66089.1"
                     /translation="MAETYNGYCVKCREKRDFEGEVKELANGSKAAQGQCPVCGTKMT
                     RMLPKNA"
     gene            2005..2766
                     /locus_tag="VNP73_08970"
     CDS             2005..2766
                     /locus_tag="VNP73_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWL66090.1"
                     /translation="MSDDLVLIETSGDIATVTINRPQRRNALDSTTAAAVRAAIEETS
                     GSRAVVLTGAGKSFCAGGDLEELERWTDLPTGDIAGTLYDSYQGMVRAVRASDAIVIA
                     AVNGAAVGAGLDLALACDLRVAAQGAKLGQVWVGLGVIPGTGGAWFTQALAGPTKAAE
                     LLLTGELISAEDALAAGLVNEVVPPDDLLSAARRWAERILKHPREGVVANKRAMIEAT
                     EPFVSAALDHAKKVQPERFTSDEFKAAVKARLSRS"
     gene            complement(2763..3554)
                     /locus_tag="VNP73_08975"
     CDS             complement(2763..3554)
                     /locus_tag="VNP73_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWL66091.1"
                     /translation="MEALKLSPSSVTDFKSCPQLFKYRTIDRVPEPVSGPAAKGSLIH
                     AVLERLFMEPGPRRIPRRAEALLEALWRQVRDDPDFRPNGMGSLEEDAWLEESRTLLL
                     NYFKLEDPRRLEASKLEWWIEYELTDMQLRGIIDRLEERDDGSWILTDYKTGRVPGES
                     REMAAFFGLRFYALVCWRAFGVIPKEIRLVYLADPVVLTLNPHERMLLAFERQMNALG
                     AAVRRALVTGDWRTRPSPFCMSCSFQSICPAWAEVTEASGATEAD"
     gene            complement(3596..>4298)
                     /locus_tag="VNP73_08980"
     CDS             complement(3596..>4298)
                     /locus_tag="VNP73_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWL66092.1"
                     /translation="NMWEPQIESLRDRFRFITVDLQGFGGSDAPDDPGTYSVDTFADQ
                     GAAVLDAAGVDKANVVGLSMGGYISFSFWRRHRDRISAFVLADTKAEADPPEGVEKRT
                     TQQQLVRDEGTDPLIEGLVGALLAEPTREKKPDVVEKVRSLMQENPAAGFIGALEAMK
                     KRPDSTDVLSGIDVPTLVIVGENDGITPPDAARKIHEHVGGSRLVVIPEVGHLSNLEA
                     PEAFNGAVAEFLSGL"
BASE COUNT          755 a         1396 c         1356 g          791 t
ORIGIN      
        1 tgaattcgcg aatcgcttcg caagtcatct tggttatgcc aaggatctat ctgagattcc
       61 atctcgtagg ggtgtgaaga agctggctca cccggtgaag gtgatctcgg tcgaatacgt
      121 tggacttgag caaacgtacg acctctgcgt tgaagggccc tggcacaact tcgtcgccaa
      181 cggatttgtg gtccacaatt ccttcaatga agaatccgcg aggtaccaca agctgtcgga
      241 cgatttctac gtgcctgacg tcccggcggt tcgctcgcag gtggggaagc cgggttcata
      301 ttcgttcgaa cctgtcgatg aagccctcgc caacgagacc agggatacct tccagcgcat
      361 ctacaaggag ctttacgtcg aatacaacga gctaatagaa aagggcgtgg ctaaggaagt
      421 ggcccggtcc atccttccgg ttgggatctt cactcagttc tactggaccc tgaatgcccg
      481 ggctctcatg aacttccttt ccctccgcaa cgcccaaacc gcccagtatg agatccggat
      541 gtacgcagat gctgtcgagc gactcttcgc acagcagatg cccatcaccc atcagtgctt
      601 catagagttc gggcgcgttg cgccctgatg tgatcctctc cgcggccgcc ttcaagttcg
      661 ggatcgatcc actcgcgtgg aagatcgttc ccgcgctcga actcggcccc ctctctatct
      721 cgccgcacgg catcggcata gcggtcggtt tccttctcgg cgcgcagctg atggtgaagc
      781 gcgccacgaa ggacacgtgg ccggtcgaga acgacatctg gaacacactc ttctgggcgt
      841 tgatcggggc gatcgtcgga gcccgcgtcg gctacgtgat cggacacctt cccgaggtca
      901 cctcgaacgg cgacgacatc ctcggcgtgt tgcgagtgtg ggaggggggc atctccctga
      961 tcggagggat caccggagcg atcctgttcg cgctgccgta catgatccgg cacaagatgg
     1021 gcttctggcg gacgatggat cttgccgccc ccggcctcgc actcggcatc atcatcggca
     1081 ggatcggcga cctcatcatc ggagaccatc tcggcaagcc gaccgacatc gccctcgggt
     1141 ggagatgtct cggaacgatc caaggggacg agccgctcgt cgccgagcgt tacctggcgg
     1201 ccgcccggct cggtagccac ccgtcgcttg gctgcttcga cgtcgcgctg caccagaccg
     1261 ctctctacga cttcttctcc acgatccttc tgctcggtgt ccttctggcg ctgggccgcg
     1321 cggcacgccg tacgggcttc ctgatcctcg tgttcaccgt ttggtacggc gcgatgaggg
     1381 tgttcaccga cttccttcgg gtcgaccggc gctacctcgg gcttaccggt agccaactga
     1441 tgtcgcttgc ggtcggactt gcgtgtctgt acctgctggc gcgctatcga ggagcgccgc
     1501 cggggtggat caggcccctt gcacccggcc ccgaaggggg tccgccggcc ggcgatcgtg
     1561 agccacttgt gcccgccggg gccccgcccg cggcctcgga aaagctacct atcgcggaga
     1621 gtgagcaaga aaccccgatt ttaggggcgg acgacgactc tccgtagtcg aaaatccgcc
     1681 cgaggccccc tggaggtggc acgattacct cacgtcctag gggggcaacc cctcgtacca
     1741 ggccccctgt agggcgggag cctgagagag gagtatggag atggcggaga cctacaacgg
     1801 ttactgcgtc aagtgccgtg agaagcgtga cttcgagggt gaagtcaagg agcttgccaa
     1861 cggctccaag gccgctcagg ggcagtgccc cgtgtgcgga accaagatga ccaggatgct
     1921 tccaaagaac gcctagcgtc tcttgacggg tgttggagag gccgcctacg ggcggcctct
     1981 gcgcgtgggg gtagtttccc gcccatgtcc gacgaccttg ttctgatcga aacctccggc
     2041 gacatcgcga ccgtcacgat caatcggccc cagcgccgca acgcactcga ctccacgacc
     2101 gccgccgccg tgcgggcggc gatagaggag acctcgggca gccgagcggt cgttctgacg
     2161 ggcgccggca agtccttttg cgccgggggc gacctggagg agctcgagcg ctggacggac
     2221 cttcccacgg gtgacatcgc cgggaccctc tacgacagct accagggcat ggtcagagcc
     2281 gtacgggcgt cggatgcgat cgtgatcgcc gccgtgaacg gcgcggccgt gggagccggg
     2341 ctggatctcg cgctcgcgtg cgacctgcga gtagcggcgc agggcgcaaa gctgggtcag
     2401 gtctgggtcg ggctcggcgt gatccccggc acgggtggag cgtggttcac ccaagctctc
     2461 gcgggcccca cgaaggcggc cgagctactg ctgacgggcg agctgatctc ggccgaagac
     2521 gcgctcgccg cagggctggt caacgaagtc gttcctccgg acgatctgtt atcggccgcg
     2581 cgccggtggg ccgagcggat cctcaaacac ccacgcgagg gcgtcgtcgc caacaagaga
     2641 gcgatgatcg aagcgaccga gccgttcgtc tctgcggccc tcgatcacgc caagaaggtt
     2701 caaccggagc gcttcacgtc ggatgagttc aaagcggccg tgaaggcccg cttgtcgcgc
     2761 tcttagtcgg cttcggttgc tccggaggcc tcggttacct cggcccatgc gggacagatg
     2821 ctctggaacg agcacgacat gcagaacggc gacggtctcg tgcgccagtc acccgtcacg
     2881 agggcccgtc taaccgcggc ccccagtgca ttcatctgcc gctcgaatgc cagcaacatc
     2941 cgctcgtgcg ggttgagagt caacacgacg ggatcggcga gatagaccag cctgatctcc
     3001 ttggggatga cgccgaaggc tctccagcac accagcgcgt agaacctcag cccgaagaac
     3061 gccgccatct cgcgcgactc gcccggcacc cgcccggtct tgtagtcggt caggatccag
     3121 gaaccgtcgt cccgctcctc gagtcggtcg atgatccctc gcagctgcat gtcggtcagc
     3181 tcgtactcga tccaccactc gagcttgctg gcttccaacc ggcgggggtc ctcgagcttg
     3241 aagtagttga gcagcagggt ccggctctct tccagccagg cgtcctcttc cagggacccc
     3301 atcccgttgg gccggaaatc ggggtcatcc cgcacctgcc gccacaatgc ttccagtaac
     3361 gcctcggctc ggcgggggat cctgcggggg ccgggctcca tgaacaaccg ctccagcacg
     3421 gcgtggatca acgagccctt cgccgcgggg ccggagacgg gttccggaac ccgatcgatc
     3481 gttcggtatt tgaagagttg aggacagctc ttgaagtcgg tgacgctcga gggcgagagt
     3541 ttgagggcct ccatcccccc aaaccctagg ggggcgatgc ccccgagagt cggatttaga
     3601 gaccgctcag gaactccgcc acggccccgt tgaacgcctc gggagcctcg aggttcgaga
     3661 ggtggccgac ctcggggata acgaccaggc gggacccgcc gacgtgctcg tggatcttcc
     3721 gagctgcatc cggcggagtg atcccgtcgt tctctcccac gatcacgagc gtcggtacgt
     3781 cgatcccact aaggacgtcc gtcgaatccg gtcgcttctt catcgcttcc agcgccccga
     3841 tgaaaccggc cgccggattc tcttgcatga gtgagcgcac cttctcgacg acgtcgggtt
     3901 tcttctcgcg tgtcggctcc gcaagaaggg cacctaccaa accctcgatc aaggggtcgg
     3961 tgccctcgtc tcgcacgagc tgctgctggg tcgtgcgctt ctcgacgccc tcggggggat
     4021 cggcctctgc cttcgtgtcg gctagaacga acgcagagat ccggtcacga tggcgtctcc
     4081 agaacgagaa ggagatgtag ccgcccatcg acagccccac gacattggct ttgtcgacac
     4141 cggcggcgtc cagcaccgcg gccccctggt cggcgaatgt gtccaccgaa taggtgccgg
     4201 gatcgtcggg ggcatccgac ccaccgaacc cctgcaggtc gaccgtgatg aagcggaagc
     4261 ggtctcgcaa ggactcgatc tgtggctccc acatgttc
//
LOCUS       DATMED010000348         2960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2600781, whole genome shotgun sequence.
ACCESSION   DATMED010000348 DATMED010000000
VERSION     DATMED010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600781"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..2128
                     /locus_tag="VNP73_08985"
     CDS             374..2128
                     /locus_tag="VNP73_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWL66093.1"
                     /translation="MKRLLAVVAACVLVLSTAGSLVAAQPLDDPIPSPIREGGVQVRL
                     EPVATGLTAPNWGISAPGDADRLFVVDQDGIVWAVDLSSGAKSVFLDVSTRLVPLGAF
                     GPNTFDERGLLGLAFDPDYANNGLVYTYTSEPVSGSADFSTMPTGTPANHQSSIAEWH
                     VPNPRNPSSVVDPSTRRELLRIDEPQFNHNAGALNFGPDGFLYISLGDGGAADDQGVG
                     HSPQGNGQDPSNVLGSILRIDPAGTNSANGNYGVPQDNPFVGRAGFVPEIWAYGFRNP
                     FRFSFDTGTGKLWTGDVGQNDIEEVDVVTKGGNFGWRVKEGSFLFDPNGAQPGFVTAR
                     SPGKPKDLIDPVAEYDHDEGVAVVGGFVYRGDAIPQLRGRYVFGEFSQSSTGFGRLFF
                     LDPADRIREFRLVGQESLGLKLDGFGQDADGELYVLGNTTGTPFGETGVVLRIAPRQR
                     DNELEADLNGFAEVDPVSGDFGAGDLNGDGLAEIRLRPDDGRVCFKVEWADIDEPVAG
                     HIHPGVVGVNGPVVVDLLGNADNFRHRDGAGSANGCAEGVSPALIDDIGNHPGAYYVN
                     IHTAAFPGGAIRGNLERD"
     gene            2323..2910
                     /locus_tag="VNP73_08990"
     CDS             2323..2910
                     /locus_tag="VNP73_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66094.1"
                     /translation="MFNVTFGRRRGVIVLLGTLAAVLALSTPASAERSFFFEETAEAF
                     WAVPHECADGSIVEATLLVRSTRDFESPETEDADPTARVQYLAVCPDGSSFSWAAPAA
                     AATITSAPNLKSVTVTGTGIARDNLGGTHEVSFDVAWTSVGPLVTTVNGPGSKRQQRE
                     AIATGQVIFDDDVLVDGEANHPTRPDPFIRVDTEK"
BASE COUNT          565 a          887 c          969 g          539 t
ORIGIN      
        1 ggccttggac ggcctgatcg agtagtcatc ggaaggcctg ctcacgaagg cccccggatc
       61 cttgcggatt cgggggcctt ccttttttcg gggtcggact gtcgggatcg tttcgagtga
      121 agttagccgc atgccggctg atctcccaga ggagcgcagt tgaccatgcc gtgcgctgga
      181 tggcccgcag accgaacgag cgcgcgagaa ggcaaccgac ggacgacatt ttccgagata
      241 tcgaaaccga gtcccctcaa ggccaggtac taggaagtga cgctgcgggc gagaggtcag
      301 ccgactggat gaaaaaaagc agaggcctcc aagcgctgcg gacattcata gggtgcatga
      361 gagggggaga ctcatgaaac gactcttggc tgtagtcgcg gcgtgcgtgc tggtgctctc
      421 gaccgcgggt tcactggttg cagcgcagcc actggacgac ccgatcccga gccccattag
      481 agagggcggc gttcaggtca ggctcgagcc ggtcgccacc ggcctcaccg ctccgaactg
      541 gggaatctct gcgccgggcg acgcggatcg actattcgtc gtcgatcagg acggcatcgt
      601 atgggccgtc gacctgtcgt cgggcgcgaa gagcgtcttc ctcgacgttt cgacccgtct
      661 cgtcccgctc ggagcattcg gtccgaacac cttcgacgaa cggggtctgc tcgggctcgc
      721 cttcgatccc gactacgcga acaacggcct cgtctacacg tacacctctg agccggtatc
      781 ggggtctgcc gacttctcga cgatgccgac cggcacgccg gcgaaccacc agagctcgat
      841 cgccgagtgg catgtcccga atccgcgcaa cccgtcctcg gttgtggatc cgagcactcg
      901 tcgtgagctt cttcgcattg acgagccgca gttcaatcac aacgccggag cgctcaactt
      961 cggaccggat ggcttcctct atatttcgct cggcgatggc ggcgcggccg atgatcaggg
     1021 cgtcggtcac agcccgcaag gaaacgggca ggatccaagc aacgtgctgg gctcgatcct
     1081 tcgcatcgac cccgccggaa ccaattccgc caatggcaac tacggagtcc cccaagacaa
     1141 tccgttcgtc ggccgggcgg gcttcgtgcc ggagatctgg gcctacggtt ttcggaatcc
     1201 cttccggttc tcgttcgaca cgggcaccgg caagctctgg acgggagacg tcggacagaa
     1261 cgacatcgag gaagtcgacg tcgtgaccaa gggcggcaac ttcggttgga gggtcaagga
     1321 aggttccttc ttgttcgatc ccaacggtgc ccagcccggc tttgtcacgg ccaggagccc
     1381 gggcaagccg aaggatctca tcgatcccgt cgcggagtac gaccacgacg agggcgtcgc
     1441 ggtggtagga ggtttcgtct accgtggcga tgccatcccg cagctcagag gacgctacgt
     1501 attcggggag ttctcgcaga gctctaccgg gttcgggcgg ctgttcttcc tcgatccggc
     1561 cgaccgaatc agagagttca gattggtcgg acaggagtcg ctgggtctga agctcgacgg
     1621 attcggacag gatgccgacg gtgagctgta cgtgctgggc aacaccaccg gcacgccctt
     1681 cggcgagacc ggcgtcgtac tcaggatcgc gccccgccaa cgagacaacg agctcgaggc
     1741 cgacctcaat ggtttcgccg aggtcgatcc cgtcagcggc gactttggag caggcgacct
     1801 caacggcgac ggtcttgcgg agatccgcct ccgtccggat gatggccgcg tctgcttcaa
     1861 ggtcgagtgg gccgacatcg acgaacctgt ggccgggcac attcatcccg gggtggtggg
     1921 tgtaaacggg cctgtcgtcg tggatctctt gggcaacgcc gacaacttcc gacaccggga
     1981 tggagcgggc agcgccaacg gatgtgcaga gggggtgtct ccggcgttga tcgatgacat
     2041 cgggaaccac ccaggtgcgt actacgtgaa catacatacg gcggccttcc cgggaggagc
     2101 gataagggga aatctggaac gagactagat cggctctcaa ccgggcccag gacctcccgg
     2161 tcaatgattt gagtgtcttt aggagtcctc gagaccggga ggtcaaatgg gtttggtacc
     2221 cgctcctgaa tcgtgttgga gcgcgtaaca agacggatcg aacagcgtta gtcagtggac
     2281 accggagcgg caacagcgcc gcatttagtg ggggtttcaa acatgttcaa tgtaactttc
     2341 gggcggcgac gcggggtcat cgtcctgctg gggacgcttg cggccgtcct ggcactctcg
     2401 actcccgcgt cggcggaaag gtcgttcttc ttcgaggaga cggccgaagc cttttgggcg
     2461 gtgccgcacg aatgcgccga cggctcgatc gtggaagcaa cgcttctggt gcgaagcacg
     2521 cgcgacttcg agtcacccga gaccgaggac gcggacccca cggccagggt gcagtacctg
     2581 gcggtctgcc ccgatggctc ctcgttcagc tgggctgcac cggctgccgc ggcgaccatc
     2641 accagcgctc cgaacctcaa gagcgtcacc gtcacgggaa cgggaatagc gagggacaac
     2701 ctcggcggaa cccacgaggt ctctttcgat gtggcgtgga cgagtgttgg accgttggtg
     2761 acgacagtca atggtccggg gagcaagagg cagcagcgtg aagcgatcgc gaccggtcag
     2821 gtgatattcg acgatgatgt cctggtcgac ggcgaagcaa accacccgac gcggccggat
     2881 ccgtttatcc gcgtcgacac agagaagtaa ccacagtggt cgttaagggc tgatcttctc
     2941 gctcgatcta gagactgcct
//
LOCUS       DATMED010000349         6393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5655522, whole genome shotgun sequence.
ACCESSION   DATMED010000349 DATMED010000000
VERSION     DATMED010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5655522"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VNP73_08995"
     CDS             <1..819
                     /locus_tag="VNP73_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HWL66095.1"
                     /translation="LREVRGAGFDRISIGVQSTVDRVLLGLGRTHSGPTALDALQAAR
                     RAGFDNINADLIYGSPWETEDDWIVSLNDVIEAEPDHVSAYALTVEEGTPLAVRVATG
                     RVPDVDPDIQAARHRVADEMLGEAGFDRYEVSNWSRPGRACRHNLLYWRAGNYAAFGA
                     GAHGHKDGTRWWTVRLPRTYIDRVRSHASTREGEERLTPDQQAGEALMLGLRLKNGVK
                     SSDFLARFGATAWDRRSGILQELHDRGLIVLDDGTVRISPDATMLSNEVLCRVL"
     gene            829..1947
                     /locus_tag="VNP73_09000"
     CDS             829..1947
                     /locus_tag="VNP73_09000"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HWL66096.1"
                     /translation="MSDTDRRIADLITDLAPMRDAADLPDLFAALARSVVRTLSADAC
                     LVSLCDDEAGELRDVAASVVPPAKMNTVVETYKLEEYPVTEAVIKAQTHAELSIHDPA
                     VDKRERTVMEEMGFARVLMCGFSIEDEVAGTVEAYRLQDRPFRTDDPEQIDLLIAFAG
                     NHYSRIQLASRLEAHYTETMEALTSALEARDPYTEAHVGRIRDLAIALGLALKVSPDV
                     RKSIHLGALLHDVGKIGISDAILQKDGPLSDEEWTIMRRHPEIGERMLRRVDFLRPAL
                     SVIRHHHERWDGKGYPDGLQGEDIPLAARIVAVCDAFDAMTSDRPYRRATTAELACDE
                     IASHAGSQFDPRCAQLLIRVVREIGQEDLEEKFVRFAS"
     gene            complement(1962..2315)
                     /locus_tag="VNP73_09005"
     CDS             complement(1962..2315)
                     /locus_tag="VNP73_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWL66097.1"
                     /translation="MDVQTAFEQRSTVLFLDVRERYEWDAGHVADSIHIPLMELPQRV
                     AEVPDTRKVIVVCQVGQRSELAAKFLRDQGYEAHNLEGGLARWQAAGLPFESAGGKGE
                     VVDGYARDFDGLINP"
     gene            complement(2326..3537)
                     /locus_tag="VNP73_09010"
     CDS             complement(2326..3537)
                     /locus_tag="VNP73_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWL66098.1"
                     /translation="MRSIRFAGHLGAALVGVIVGGLVVLGVEGMTRTPSTSPSRQPSS
                     GPIDVVPDYRTAVPNVMLAWSPLGLPAELDDRLDGMRGVSAATMVHSGLVWMVEARDG
                     DRAIIERHTEGWAVPLEVAIVDPDQYARFVPRSERARILALDRDTALLSRSAASLRRA
                     EQGASLTLSEGRRVKVAGVVSDSTAQGYEMLLAGPPPTTWSRTDPAVLALIPNRAARA
                     RVEKKVRGILGSGHVVRIRAEGETPYLRYGDAVLPQLHVKQVFGEFAARPSGGGTIQV
                     DPRWRSVSIASQRVPLVGRVACHVAVFDQLRAALREVIAAGLSFTVNKEQFGGCYSAR
                     YINHDPAGRLSHHSWGMAIDLNVAENSFGSRPDQDRRLVEIFERHGFTWGGRWLIPDG
                     MHFEWVRGASS"
     gene            complement(3534..5360)
                     /locus_tag="VNP73_09015"
     CDS             complement(3534..5360)
                     /locus_tag="VNP73_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWL66099.1"
                     /translation="MRSELRCDDVRVELSARLDGEIDARTEELVVEHLGTCRECRAHE
                     ERLQRIRHSIRVQPSPRVPDLTRSIMDRVASRPVGRGFAWPQIRTAAIAAAATALIVL
                     GASLPGSDAPPDTARAAEIVRSIKTAARTLTSYHATFTVTELGWHPDVLRRRFSSEVW
                     WRAPEKFRLQVRDLTVYPNADWPRNDIDLIASPRRYRIEEPSTCPTAALPRCSVFQGV
                     ETRTIVDRQPFDGNSRLPTDIALPLETLSSAEGLEVVEPGTYLGRDVERVRLPVAVAL
                     PLLRSIQVGGDWRAFHLRDRVELWIDRATWFPLRFIVTAGSSSDRSLWAQRMGYVSDR
                     PGAELLDVQATRFSESNDFDPALFRSRVEGAVLDGGFTAQAVPGDLAAGVAGLEPYRV
                     GSVRSQTISTFATGMYFLKVTSDRDRVGSAADLIDGSVVKVGEGFGLYHPASERSARK
                     IDLFTRRGRTTLETNLPRTQLLRIAAPMASGSVIPSKIDGVLRLTETHLARTKALVPS
                     WMPEGFVRTSALMSTADRTVTITYQRPETSFAGIRITQTPLVRRLQPTAEQTIAIELD
                     DVVARWSFERSELEWIDGNVYRAVATSGGDLATAVQIARSLR"
     gene            complement(5350..5928)
                     /locus_tag="VNP73_09020"
     CDS             complement(5350..5928)
                     /locus_tag="VNP73_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWL66100.1"
                     /translation="MPIVHQPVEDDALFEPDPAVVRAARAGDSSAFEELVRAYQADVW
                     RLCLHLVRNEALADDVTQDAFVRAFRFLKRYRGESKFSTWLFSIAHNCAVDEIRRAGR
                     RRRTQELLHAQRPAEPQDPAASMEIRYAIESLPIDLRAPMLLIDIFGLPYREAGRILK
                     LPEGTVKSRVHRARVSVAEMLEPKQKRANDEI"
     gene            5988..6284
                     /locus_tag="VNP73_09025"
     CDS             5988..6284
                     /locus_tag="VNP73_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66101.1"
                     /translation="MAYVGNLICQDCGLTFTARWGSFAHADEYRCENDHVLHVEPDSG
                     EVVAVEGGSVGGRTLADFFGRCPLCDTELACGRMPSCPVCRGRDHEVLLAGTLD"
BASE COUNT         1156 a         2027 c         2061 g         1149 t
ORIGIN      
        1 ctgcgggagg ttaggggggc cgggttcgat cgcatctcga taggggtcca atcgacggtc
       61 gaccgtgtgt tgcttggtct cggccgcacg cacagcgggc cgaccgcgct tgacgcgttg
      121 caggccgctc gacgcgccgg attcgacaac atcaatgccg atctgatcta cggctcaccg
      181 tgggagaccg aggacgactg gatcgtcagt ttgaacgacg tgatcgaggc cgagccggat
      241 cacgtgtccg cttatgcgct gaccgttgaa gaaggcacgc ccctcgccgt tcgcgttgct
      301 accggtcgtg tgcccgacgt cgatcccgat atccaggcgg cgcgtcatcg agtcgccgac
      361 gagatgctgg gcgaagccgg ctttgatcgg tacgaggtat cgaactggag ccgacccggc
      421 cgggcatgcc gtcataacct cctctactgg cgcgcgggga actatgcggc cttcggtgcg
      481 ggcgctcacg ggcacaagga tgggacgaga tggtggacgg tcaggctgcc gcgcacctac
      541 atcgaccgtg tgcggagcca cgcttccact cgcgaggggg aagaacgttt gacccccgat
      601 cagcaggccg gagaggctct gatgctggga ttgcgactaa agaatggtgt caaatcgtcc
      661 gattttctcg cgcggttcgg cgcgacggca tgggatcggc gcagcgggat cttgcaagag
      721 ttacacgatc ggggactgat cgtgctcgat gatggcacgg ttcgcatcag cccggatgcg
      781 accatgttgt cgaacgaagt tctctgccga gttctgtagg agattcgctt gtcagatacc
      841 gaccgcagga tcgccgatct cataacggac ctggctccga tgcgggatgc agccgacctc
      901 cccgatctgt tcgctgctct cgcgcgatcg gtggtccgca cactcagcgc ggatgcctgc
      961 ttggtttcgc tgtgcgacga cgaggccggc gaactccgtg acgtcgctgc gagcgtcgta
     1021 ccccctgcga agatgaacac ggtcgtcgag acctacaagc tcgaggagta tccggttacg
     1081 gaagcggtca tcaaggccca aactcacgca gagctatcga tccacgaccc ggctgtcgat
     1141 aagcgtgaga gaacggtcat ggaggagatg ggtttcgcca gggtgctgat gtgcggtttc
     1201 tcgatcgagg acgaagtcgc ggggacggta gaggcttatc gtctacaaga tcggccattc
     1261 cgcacggacg accccgagca gatcgaccta ctgatcgcat tcgccggaaa ccactattcg
     1321 aggatccagc tcgccagcag gttggaggcg cactacaccg agacgatgga agccctcacc
     1381 tcggctctag aggcgcgtga tccctacacc gaggcgcacg tgggcaggat ccgcgacctg
     1441 gcgatcgcgc tggggttggc cctcaaggtg tctcccgacg tacgaaagtc gatccacctc
     1501 ggcgctttgc tgcacgacgt cggaaagatc gggatctcgg atgccatcct tcaaaaggac
     1561 gggccgctgt ccgacgagga atggaccatc atgcgacggc atcccgagat cggcgaacgg
     1621 atgctgcgcc gggtcgattt tctccgccct gcgctttctg ttatccggca tcatcacgaa
     1681 cgctgggacg gaaagggtta ccccgacggt cttcaaggag aggacatccc gttggccgca
     1741 cggatcgttg ccgtgtgcga cgcgttcgac gcgatgacgt cggaccgacc gtaccggcgg
     1801 gctacgaccg ccgaactagc ttgcgacgag atcgcatccc acgccgggtc gcagttcgat
     1861 ccgcgctgcg cgcagttgct cataagggtc gtgcgggaga ttggacaaga ggatctcgag
     1921 gagaagtttg tccgcttcgc atcctgacct agggcccgcg tctaggggtt gatcaatccg
     1981 tcgaagtcgc gggcgtagcc gtctacgacc tctccctttc ccccggcaga ttcgaacggc
     2041 agtccggcgg cctgccatcg cgccagaccc ccctcgaggt tgtgagcctc ataaccctgg
     2101 tcgcgcaaga acttggcagc gagctcgctg cgttgaccga cctggcacac cactatgacc
     2161 ttgcgagtgt cgggaacttc ggccacgcgc tgcggcaatt ccatcagcgg gatgtgaatg
     2221 gaatctgcaa cgtgacctgc atcccactcg tagcgctcgc ggacgtctag gaataggaca
     2281 gtggatcgtt gttcgaaagc cgtctgcacg tccatcgtcg agatgctagc tgctcgcccc
     2341 ccgcacccac tcgaaatgca tcccatcggg gatcagccac cgtccgcccc aggtgaagcc
     2401 gtggcgttcg aagatctcga ccagccgcct gtcctgatcg gggcgagaac cgaaggagtt
     2461 ctccgcgacg ttgagatcga tcgccatccc ccacgaatgg tgtgagagcc ggcccgcggg
     2521 atcgtgattt atatagcggg cgctgtagca gcctccgaac tgctccttgt tcacagtgaa
     2581 cgacagcccg gccgcgatta cctcgcgtaa tgccgctcgc aattgatcga agaccgcgac
     2641 gtgacaggcg acgcgcccca ccagcgggac gcgctgggac gcgatgctca ccgaacgcca
     2701 cctggggtcg acctggatcg tgccgccccc cgatggtcgt gcggcgaact ctccgaagac
     2761 ctgcttcacg tgtagttggg ggagcacggc atcgccgtat cgcagatagg gcgtctcacc
     2821 ctcggcccgg atccggacga cgtggccgga accgaggatg ccgcgcactt tcttctcgac
     2881 gcgtgctctc gctgctcggt tggggatgag ggccaggacc gcgggatcgg ttcggctcca
     2941 cgtagtcgga ggcggaccgg cgagcagcat ctcgtatccc tgggcggtgc tgtccgatac
     3001 gactccggcg accttcaccc tgcggccctc gctcagagtc agcgacgcgc cctgctcggc
     3061 tcgtcgtaag gaggctgcac tccgggacaa caaagcggtg tcgcgatcga gggccaagat
     3121 ccgagcgcgc tccgaacgtg gcacgaagcg tgcgtactga tccgggtcga ctatcgcaac
     3181 ttcgaggggc actgcccaac cctccgtatg gcgttcgatg atcgcccgat ccccgtcccg
     3241 agcctcgacc atccacacca gtcccgaatg gaccatcgtg gctgcactaa cgccgcgcat
     3301 cccatccaaa cgatcatcga gttcagccgg gagcccgagt gggctccacg cgagcatcac
     3361 gttcggcact gccgtccggt aatccgggac cacgtcgata ggtccgctcg acggctgtct
     3421 cgaaggagac gtgcttggtg ttcgcgtcat gccctcgacg cccagcacga cgagcccacc
     3481 gacgatgacg ccaacgagag cggctcccag atgcccggca aaccggatgc ttctcatcga
     3541 agggaacgag cgatctgcac cgcggtggcg agatcgcctc ccgaggttgc gacggcccgg
     3601 tagacgttgc cgtcgatcca ctccagctcc gaacgttcaa aagaccatct ggcgacaacg
     3661 tcatcgagtt cgatcgcgat cgtttgttcg gcggtgggtt gcaagcgacg tacgagaggt
     3721 gtctgtgtga tgcggatgcc tgcgaagctc gtctcggggc gctggtaggt gatggtcacg
     3781 gttcggtcgg ccgtcgacat cagagcggac gtccgtacga agccttccgg catccatgac
     3841 ggtacgagcg ccttcgtgcg agcgagatgt gtttccgtga ggcgaagaac gccatcgatc
     3901 ttggatggga tcacgctgcc ggacgccatg ggggccgcga tgcgaagaag ctgcgtgcgg
     3961 ggcaggttcg tctcgagcgt tgtgcgtcca cggcgggtaa agagatcgat cttgcgggcc
     4021 gaacgctcgc tcgccggatg atagagaccg aacccctcgc cgaccttgac gacggatccg
     4081 tcgatcaggt cggcagccga acccacccga tcacgatcac tggtcacttt caggaaatac
     4141 atgcccgtgg caaaggttga gatcgtctgc gatctcacgg atcccacgcg gtaaggctcc
     4201 aatcccgcca cgcccgcagc gaggtcgcct ggtaccgctt gagcggtgaa gccaccgtcg
     4261 agcactgccc cttcgacccg gctccggaac aaagcgggat cgaagtcgtt cgactcggag
     4321 aatctcgttg cctgaacgtc cagtagctcg gctccgggcc gatccgagac ataacccatg
     4381 cgctgcgccc atagcgatcg gtccgacgag cttccggccg tgacgatgaa acgcagaggg
     4441 aaccaggtcg ctcggtcgat ccacaactcc acccgatccc gcagatggaa ggcccgccag
     4501 tcgcccccaa cctggatcga gcgaaggaga ggcaacgcga ccgctaccgg tagccgcacc
     4561 cgttcgacat cacggccgag gtacgtcccc ggctcgacta cctcgagtcc ctccgcgctc
     4621 gacagcgttt cgagcggcaa tgcgatgtcg gtcggcagtc ggctgtttcc gtcgaagggc
     4681 tgccggtcga cgatcgttct ggtttcgact ccctggaaca cggaacaacg cgggagtgcc
     4741 gcggtcgggc atgtcgacgg ttcttcgatc ctgtagcggc gtgggctcgc gatcagatcg
     4801 atgtcgttgc gcggccagtc cgcgttcggg taaacggtga ggtctcggac ctggaggcgg
     4861 aacttctccg gagcgcgcca ccacacctct gacgagaatc gcctgcgcag cacatccgga
     4921 tgccatccga gctcggtcac cgtgaaggtc gcgtggtacg aggtcaaggt gcgagcggca
     4981 gtcttgatgc tccgcactat ctcggcagcg cgcgccgtgt cgggtggggc atcgctaccg
     5041 ggcagcgacg ctccgagcac gatcagggcc gtcgccgcag cggcgatcgc ggccgtccgg
     5101 atctggggcc acgcgaaccc gcgtccgacc gggcgcgagg cgactctgtc catgatcgat
     5161 ctcgtgaggt cggggacgcg ggggctcggc tgcacgcgga tcgaatgccg gatgcgctgg
     5221 aggcgttcct cgtgcgccct gcactcacga cacgtcccta ggtgctcgac aacgagctcc
     5281 tcggtacggg cgtcgatctc gccgtcgagc cgcgccgaca actcgaccct tacgtcgtcg
     5341 caacgaagtt cagatctcat cgttcgccct cttctgtttc ggttcgagca tctcggcaac
     5401 ggagacgcgc gcacgatgca cgcggctctt tacggtgccc tcgggaagtt tcaggatgcg
     5461 tccggcttcg cggtacggca gcccgaagat gtcgataaga agcatcggcg cccgaaggtc
     5521 gatcggcagg gactcgatcg cgtacctgat ctccatcgag gctgccggat cctgcggctc
     5581 ggccggccgt tgtgcatgaa ggagctcttg ggttcgtcgt cgtcgtccgg cccggcggat
     5641 ctcgtccacc gcgcagttgt gagcgatcga gaacagccag gtcgagaact tggactcacc
     5701 tcggtaccgc ttgaggaagc ggaaggctcg tacgaaggca tcctgagtga cgtcatcggc
     5761 gagggcttcg ttgcgcacga gatgcaggca cagcctccac acatctgcct ggtaagcccg
     5821 gacgagctct tcgaaggcag aggagtctcc ggcgcgtgcg gcccgcacta cggcggggtc
     5881 gggctcgaac aaggcatcgt cctccaccgg ctggtgaact atgggcatcg tcgcgctgtg
     5941 agacgctcgc tggagcacaa tggttcggcc gtgccatgct ttagggcatg gcttacgtcg
     6001 gcaacctgat ctgccaggac tgcgggctga ccttcacggc ccgatggggc tcgttcgccc
     6061 atgccgacga ataccgctgc gagaacgacc acgtgctcca cgtcgagccc gattccggcg
     6121 aggtcgtagc cgtcgaggga ggaagtgtcg gggggaggac gctggccgac ttcttcggcc
     6181 gctgccccct gtgcgatacc gagctggcat gcgggcgcat gccgtcctgc ccggtgtgcc
     6241 ggggccgcga tcacgaggtg ctgctggccg gaaccctcga ttgatctttt agcactcacc
     6301 ttcgcggagt gctaaaagct gcccccggag agtacgatta gggcgtgagc acccatccct
     6361 ccgatccctc cgacatcggc gagcggcgcg ccc
//
LOCUS       DATMED010000350         5688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_668675, whole genome shotgun sequence.
ACCESSION   DATMED010000350 DATMED010000000
VERSION     DATMED010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668675"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VNP73_09030"
     CDS             <1..679
                     /locus_tag="VNP73_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66102.1"
                     /translation="DGNDILTGLGGDHDVLRGGAGDDTIDGGDGSADLATFSEATASV
                     TADIDSGSASGQGADTLVDVEALTGGAGNDSLTGDGSSNHLAGGPGDDSLIGAGGFDA
                     VYFQAATQAVTAQLDTRTASGEGNDTFDAVEALIGSSFADVLRGDAAGNVVVGGSGVD
                     EIDGGAGDDLCAEVEVASNCEADTPEGHSLGDEYGAIAAFSQLQHTLSVVDKAFEEIP
                     AHPKLNF"
     gene            683..1348
                     /locus_tag="VNP73_09035"
     CDS             683..1348
                     /locus_tag="VNP73_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66103.1"
                     /translation="MSLAAPAPALQRFRSTAFIFLSLAGVAAALTSLFLGMRAVMEIG
                     GACAEGGPFVPVRPCPRGAPGFIVGGIWGGLISCAIYAWQSFKNKVPSLVAFAWPALF
                     LSLGWNFFEYGFNPPFGEGWVWGWLICGVVFFLMGGLPLLVILSPTVRNFDPNKPTDA
                     ELNRTLSGPRIVRDKLFGREAQPVITTEPPPVQGWFWLIVQFGAIAGGIWAAVWLYGV
                     VTG"
     gene            complement(1420..1881)
                     /locus_tag="VNP73_09040"
     CDS             complement(1420..1881)
                     /locus_tag="VNP73_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66104.1"
                     /translation="MAGVKFSEGRILRLIVGVFAVFALGGAMVACGGDETPQPRQFGG
                     DDDATPEEPTLPGEVGSEVKLSFKRNRFEVEVFSDRLYCLDTRGGTILEKPRRGKATK
                     VAVFKTDDTGVDSVKIKKPGGTYFARLDKNEFAKYGQVSVCSGAQSPNTRV"
     gene            complement(2123..3454)
                     /locus_tag="VNP73_09045"
     CDS             complement(2123..3454)
                     /locus_tag="VNP73_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66105.1"
                     /translation="MAKLRFVGLLLTAVMVMPSWVGATQAPEDVHSDNIHQIARAPIK
                     VPGGGNADGSDMAFEGNLLVAGSYSGTAIFKMLKEAPFVKQIGFHNCPGGQGDVNVYR
                     DLVFVSLDSPQSSDTIGCKEPSAKKSAGKEGLRIVDISNPTKPVQVNYVETDCGSHTA
                     VINPHGDTVFVYVMSYPLGAPSPTCNAASHRKVSIIEVPVDAPEKAKVVNTLDVSPEI
                     GCHDVTIFPNGKLAAAACIGESQIWDVSDPAAPVIISRIVNPGIEIHHGTGVTWDEKH
                     LAIADEFAGSVTGECVGSQNSPVGALWFYDITDPTSPQLEGYYNVPRGARPDTPDEAA
                     YMACTSHNFNILPMKDPTKYIAAIGYRSAGWSVVDFSDPTAPKELGFYHQLEGGEIPD
                     VWSAYWYNGRIYTNDNGASRGVSVYEMKGTSAPDVRYFKTRMNPQVQTPDF"
     gene            3543..4886
                     /locus_tag="VNP73_09050"
     CDS             3543..4886
                     /locus_tag="VNP73_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66106.1"
                     /translation="MNLRSLWVVAFAALIAVQALPARGQAPGVPAQVVDYEPPSSIEK
                     FKVTNFGPSASPSDDKTTKAKFRVVERTGNCCENYLTATPEGRLLDLGGSYINFTDDD
                     GKTWKSVRPANPLVNGEGTLAVAPNDDIVGIEWDPYSADHMLAYKYTAETKEWTYLEA
                     PLKTPFYDRPWLSVVPGPFTINGAEVPYVTFVDGFPHTGAFLYSTDGLTYVQGSSPFV
                     DQQGGAEVVNKLATGKEPALDWVQPNTQSPIVPLGGGSALAAPGAFQEAWNIFDPETQ
                     KWNSLQLDTGELSGRYLVDSKGRIHNLKTLGQMLEYRISTDGARTWKSTTIQLPKDHS
                     SPGGMMVDMRANASLGIAAIALHVSTGETDGDLLYKFDITGKKPRPISVVHVGLADID
                     ASAGVGQDIRMDFETLAILPDGRLVVSFLDSTTGPVMHLQEPIAERLGPAIAIEL"
     gene            4954..>5688
                     /locus_tag="VNP73_09055"
     CDS             4954..>5688
                     /locus_tag="VNP73_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HWL66107.1"
                     /translation="MFRDFERCYRAVESRDARFDGMFFTAVTSTGIYCRPSCPALTPK
                     RKNVTFFATAAAAHGAGFRACKRCRPDASPGSPEWNVRADIVGRAMRLIHDGVVDREG
                     VAGLARRLHVGERHLHRVMIEQVGAGPQALARAQRAQTARVLIETTPLPFTEVAFAAG
                     FASIRQFNDTVRAVFAATPSELRRKREAPSEANGHVSLRLPFRPPFDRRIIDFLGSRT
                     IEGVEKVDGNTYRRTLSLAHGPGIVEL"
BASE COUNT          997 a         1785 c         1828 g         1078 t
ORIGIN      
        1 tgacggcaac gacatcttga ccgggctggg cggggaccac gacgttttga gaggcggcgc
       61 cggtgatgac acgatcgacg gcggtgatgg atccgcggac ctcgccacgt tctcggaggc
      121 aacggcgtcc gtgacggcgg acatcgactc gggttccgct agtgggcagg gcgcggacac
      181 gctggtcgac gtcgaggcgc taacgggcgg cgccggcaac gacagcctca ccggcgacgg
      241 gtcgagcaat cacttggccg gagggccggg cgacgacagc ttgatcggcg cggggggatt
      301 cgatgccgtg tacttccagg cggcgaccca ggccgtgact gcacagctcg acacccggac
      361 cgcctccgga gaggggaacg acacgttcga cgcggtcgaa gctctgatcg gctcgagttt
      421 cgcggacgtc cttcgcggcg atgcagcggg caacgttgtc gtggggggat ccggcgtaga
      481 tgagatcgat ggcggagccg gcgatgacct gtgcgcggag gtcgaggtcg cgtcgaactg
      541 cgaggcggat acaccagagg gtcactcgct cggtgatgag tatggggcca tcgctgcgtt
      601 ctcccagctt caacacacgc tctcggtcgt cgacaaagcc ttcgaagaga tccccgcgca
      661 cccgaagctc aacttctagc cgatgtcgct cgccgcgccg gctcccgctt tgcaacgttt
      721 tcgctcgacc gcattcatct tcctgagtct ggccggtgtc gccgctgcgt tgacctcgtt
      781 gttcctcggg atgcgggcgg tgatggagat cggcggcgca tgcgccgagg gcggcccgtt
      841 cgttcctgtg cgaccttgcc cgagaggagc gccgggtttc atcgtcggag gcatctgggg
      901 tgggctcatc tcttgcgcga tctacgcgtg gcagtccttc aagaacaagg ttccgagcct
      961 ggtcgcgttc gcgtggccgg cactgttcct atcgcttggg tggaacttct tcgagtatgg
     1021 gttcaaccca cctttcggcg aaggttgggt gtggggttgg ctgatctgcg gagtcgtctt
     1081 cttcctcatg ggaggccttc cgctgcttgt gatcctgtct ccgaccgtaa gaaactttga
     1141 tccgaataag ccaacggacg ccgaactcaa tcggaccctc tccgggccga ggatcgtgcg
     1201 ggacaagttg tttggtcgcg aggctcagcc cgtgatcacg accgagcccc cgccggtgca
     1261 ggggtggttc tggttgatcg tccaattcgg cgccatcgcc ggtggcatct gggcggccgt
     1321 gtggctctac ggagtggtca cgggatagac gaaagaagaa aaagaagaga gggccggttt
     1381 cccggccccc tcgtgggaag tttctgacta gtcctgcgct tagacccgag tgttcgggga
     1441 ctgggctccg ctgcaaacgg acacctgccc gtacttcgcg aactcgttct tgtcgaggcg
     1501 agcgaagtag gtgccacccg gcttcttgat cttgacgctg tccacgccgg tgtcgtcggt
     1561 cttgaacacg gcaaccttcg tcgccttgcc cctgcgaggc ttctcgagga tcgtgcctcc
     1621 gcgggtgtcg aggcagtaga ggcggtcgga gaagacctct acctcgaagc ggttcctctt
     1681 gaacgaaagc ttgacctcgg atccgacctc gcccggaagc gtcggctcct cgggggtcgc
     1741 gtcgtcgtct cctccgaact ggcggggctg cggcgtctcg tcgccaccac atgcgaccat
     1801 ggctcccccc agcgcgaaca cggcgaatac gccgacgatc aaccgcaata tgcggccctc
     1861 agaaaatttc actcccgcca cttgctcctc cttgttcggt ccggccccag ttgggttctc
     1921 agtttcatcg acgctagtcg tgccggactc cagtgtctgt cgggcatcag acacatttga
     1981 aaggaatccc cagaagctga caatgggctt tgaccggttt gttcgagcga tcgccgagcc
     2041 gatcgcttgt tatgcccggt cgggcggcga cattgagcca gggcgaaaaa ccgatcgcct
     2101 aggcgatcgg tggaacggtt ggttaaaagt ccggggtctg gacctggggg ttcatgcggg
     2161 tcttgaaata gcgaacgtcc ggcgccgatg tgcccttcat ctcgtagacg ctgacacctc
     2221 ggcttgctcc gttgtcgttc gtgtagatcc ggccgttgta ccaataggcc gaccagacgt
     2281 ccggtatctc gccgccctcg agctggtgat agaagccgag ctccttgggt gccgtggggt
     2341 cggagaagtc gacgaccgac cagcctgcgc tgcggtagcc gatcgcggcg atgtacttgg
     2401 tcgggtcctt catcggaagg atgttgaagt tgtgcgaggt gcacgccatg tacgccgctt
     2461 cgtccggagt atcgggccgg gccccccgag gaacgttgta gtaaccctcg agctgaggcg
     2521 aggtggggtc ggtgatgtcg tagaaccaca gcgcgccgac cggactgttc tgactgccca
     2581 cgcattcgcc cgtgaccgaa cctgcgaact cgtctgcgat cgcgaggtgt ttctcgtccc
     2641 acgtcacacc ggttccatga tggatctcga tgcccgggtt gacgatgcgg gagatgatga
     2701 cgggggccgc gggatcgctc acgtcccaga tctggctctc gccgatgcaa gctgcggcgg
     2761 ccaacttccc gttcgggaag atcgtcacat cgtggcaccc gatctcgggc gatacatcga
     2821 gcgtgttcac gactttggcc ttctcgggcg cgtcgacggg tacctcgatg atcgagacct
     2881 ttcggtgcga ggcggcgttg catgtaggtg acggcgcgcc gagcgggtac gacatcacgt
     2941 acacgaagac cgtgtcgccg tgcggattga tcactgcagt gtgcgagcca cagtcggtct
     3001 cgacgtaatt gacctggacc ggcttcgtcg ggttcgagat gtccacgatt cgcaagccct
     3061 ctttgccggc cgatttcttc gccgatggct ccttgcaccc gatcgtgtcg gaactttggg
     3121 gagagtccag cgacacgaac acaagatccc tgtagacgtt cacgtcgccc tggccgccgg
     3181 gacagttgtg gaagccgatc tgtttcacga acggtgcttc cttgagcatc ttgaagatcg
     3241 cggtgccgct gtaggaaccc gcgacgagaa ggttgccttc gaacgccatg tcggagccgt
     3301 cggcgttccc tccgcccggc accttgatcg gggcgcgggc gatctggtgg atgttgtcgg
     3361 agtggacgtc ctcgggggcc tgggtcgcgc cgacccacga cggcatcacc atcacggccg
     3421 tcaagagcag acccacgaac ctgagcttgg ccaaaaggac ctccttacct ggggatttcg
     3481 ccgcgggccc ccgccttcct cctggggcag ggacgaaccc ctctacggcg aacctctgag
     3541 acatgaatct cagatcccta tgggtcgtcg cgttcgccgc cctgatcgca gttcaggccc
     3601 tccccgcgcg gggtcaagcc cccggggttc cggcccaggt cgtcgactac gagccaccca
     3661 gctcgatcga gaagttcaag gtcacgaact tcggcccgag cgcgtcgcct tcggacgata
     3721 agacgacgaa ggcgaagttc cgggtcgtcg agcgaaccgg caactgctgc gagaactacc
     3781 tgactgcaac ccccgaggga cgtttgctcg atctcggcgg cagctacatc aacttcacgg
     3841 acgacgacgg caagacgtgg aagagcgttc gcccggcgaa tcccttggtg aacggtgaag
     3901 gcacgctcgc agtcgcaccg aatgacgaca tcgtcggcat cgaatgggac ccctattccg
     3961 ccgaccacat gctcgcttac aaatacaccg ctgagacgaa ggaatggacg tacctcgagg
     4021 ccccgctcaa gacgccgttc tacgaccgtc cctggttgag cgtcgtgccg ggtccgttca
     4081 cgatcaatgg tgccgaggtt ccctacgtca cgttcgtaga tgggttcccg cacacgggcg
     4141 ccttcctcta cagcacggac ggcttgactt acgtgcaggg ctccagcccg ttcgtagatc
     4201 agcagggcgg tgccgaagtc gtcaacaagc tggctaccgg gaaggagccc gcgctcgact
     4261 gggtgcagcc gaacacgcag agcccgatcg ttccgctcgg tggcgggtct gcgctggccg
     4321 cgccgggtgc gttccaggag gcgtggaaca tcttcgaccc cgagacacag aagtggaaca
     4381 gccttcagct cgataccgga gaactcagcg ggcgttatct cgtcgactcg aagggccgga
     4441 tccacaactt gaagacgctt ggccagatgc tcgagtacag gatctcgacg gacggtgccc
     4501 ggacgtggaa gtccacgacg atccaactac cgaaggacca ctccagtccc ggcggcatga
     4561 tggtcgacat gcgggcgaat gcgtcccttg gtatcgcggc gatcgcgcta cacgtcagca
     4621 ccggggagac cgacggtgac ctcctctaca agttcgacat cacgggcaag aagccgcgac
     4681 cgatcagcgt cgttcacgtc ggacttgcgg acatcgatgc gtccgccggc gtcgggcagg
     4741 acatcaggat ggacttcgag acccttgcga tccttcccga cggtcggctc gtcgtttcgt
     4801 tcctcgattc gacgaccggt ccggtgatgc acctgcagga gccgatcgcc gagcggctcg
     4861 gccccgccat cgcgatcgag ctctaaaagc acctcagacg aggatgtccg ataaccgcta
     4921 gcacggcccc ctccgggatg acagcatggg ggcatgttcc gcgatttcga acgttgttac
     4981 cgagccgtcg agagccgtga tgctcgcttc gatgggatgt tcttcacggc cgtcacgtcc
     5041 accggcatct actgccggcc cagctgtccg gccctaaccc ccaagcgcaa gaacgtcacg
     5101 ttcttcgcga cggccgccgc cgcccatggc gccggcttcc gggcgtgcaa gcggtgtcga
     5161 cccgacgcgt cccccggctc ccccgaatgg aacgtgcgag cggacatcgt cggccgggct
     5221 atgagactga tccacgacgg agtcgtcgat cgcgagggtg tagcgggcct ggcgcgtcga
     5281 ctgcacgtcg gagagcgtca tctgcaccgg gtgatgatcg agcaggttgg agcgggaccc
     5341 caagcactcg cccgtgctca gcgtgcacag acggctcgcg tcctcatcga gacgacgcca
     5401 ctgccgttca ccgaggttgc gttcgcggcc ggcttcgcca gcatccggca gttcaacgac
     5461 accgtccgag ccgtcttcgc agcaacgccc agcgaactca ggcgcaagag ggaagcaccg
     5521 tcggaggcta atgggcacgt ctcgctacga cttccgttcc gtccaccctt cgatcgccgc
     5581 atcatcgact tcctcggttc tcgcacgatc gaaggagtgg agaaggtcga cggcaacacc
     5641 taccgaagga cgttgagtct ggcccacggg ccggggatcg tcgaactg
//
LOCUS       DATMED010000351         7155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1037184, whole genome shotgun sequence.
ACCESSION   DATMED010000351 DATMED010000000
VERSION     DATMED010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037184"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1130)
                     /locus_tag="VNP73_09060"
     CDS             complement(66..1130)
                     /locus_tag="VNP73_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWL66108.1"
                     /translation="MPRLRRSDCASAGYARRKRGRGFEYLNSDGTRIGDLQVLQRIKD
                     LHIPPAWIDVWICPFDNGHLQAVGTDTAGRKQYLYHTAWRQRRDQEKFDRMIEFAKAL
                     PKIRHFTNEHLDHSGLGRERVLACAARLLDRGFFRVGGEDYAEENETYGLATIRKDHV
                     RFEGRSVVFEYVAKSNKERIQSIVDPEVYEVVRALKRRRSGGPELLAYKLGSRWRDVK
                     SSEINEYLKEISGGDYTAKDFRTWHGTVLCARALAVSRGALGSKTGRKRAVSRAIQEV
                     SHYLGNTPAVCRASYVDPKVIDRYDSGVTIAPVIEKLADEDFDEPAIQVAFEEAVLDL
                     IEGNTRSPDVEKVNPAKLSA"
     gene            complement(1175..1726)
                     /locus_tag="VNP73_09065"
     CDS             complement(1175..1726)
                     /locus_tag="VNP73_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66109.1"
                     /translation="MLEEGWYLMSVADVEAVLAGRESATIKRLSTHEALAIRDAGNVP
                     DELNRSLRLVLVVDDDDPEAFARRRIEFEPDYQEQPSWRREGSKPVNVIPVRKGPRGP
                     TIDEWRDDPAVAALEEEWLESGTMAGVKVPAEYRSFVHKTVLALRATDSDVTAETIAD
                     SIARWVPPEDAARIRVALLELNG"
     gene            complement(1759..2439)
                     /locus_tag="VNP73_09070"
     CDS             complement(1759..2439)
                     /locus_tag="VNP73_09070"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66110.1"
                     /translation="MRVFSIRAIRIAVGLAMTAYYAFGLLGLPAAAHGRDGDGIPHRW
                     ESKHGTNPHMHDSGMDNDSDGLKNKGEYRHGTDPLDPDTDDDGLLDGEEVRELKTDPK
                     LSDSDGDGTSDGDEFSEDADGEDAIDCVEDDDDDDDEDEDDDEGDDEGDEDCDQHGTI
                     TSYDSGSGVLTVRLDSGETVTGTVTDDTEIDWDDETEASEDDLDPGAVLEFEMNDDGT
                     IEDIEIEK"
     gene            2609..4747
                     /locus_tag="VNP73_09075"
     CDS             2609..4747
                     /locus_tag="VNP73_09075"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HWL66111.1"
                     /translation="MANNARNRFALLGLLLGLLATTIPSLPSASAAGRAVKIRTLSNR
                     ADLISGGDALIQVSVPAGASTSDIRLSLNGKDVTGAFQEGSNGNGQGLIEGLKLGRNK
                     VVASLPDGRAASLTITNHPLGGPVFGGPQIKPWTCQEEALDKKCNQEPTISYYYKPAG
                     GGSLQPYDRDNPPPPESIAETTTTEEVTVPFIVREEIGYMARDQYAIATLWQPDKKWS
                     PSEPQRQFNRRLVITHGASCDTSYEVGEAPAVLDEAILGAGFIVMSHALDNAGHACNL
                     LMQAEALVMTKEYVIDHFGTVRWTIGMGCSGGSLVQQQVANAYPGLYQGITPQCSFTD
                     AWSSAMQYVDYYMLLAYFNDPSRWDPGTSWSPDKMAAVLDHPNIGNPVSFTSVIPNSG
                     NPSRSCPGVTADKVYNAETNPEGVRCTLQDYTVNAFGRRKKDGFANRPFDNVGIQYGL
                     KGLRQGVLTPAEFVDLNTHLGGLDIDGNFQAKRSKADLIGLKRVYRTGLVDSANNLDK
                     VAIIDLRGPDPGAFHDVYRTYAMRARLLRNFGTAANQVLWRGQVALVGDPSFAVDAVF
                     AVDRWLARVDADDRDVPLAQKIIEDKPSDIADRCTDGSGREQVSWVCDQTVASYGTPR
                     FAADAPYTDDVLKCRLQPLRKDDYSVEFTDEQWKRLQKAFPAGVCNYGKPGVHQMGAT
                     SWLTYQDARGRVIYGGRPLGPPPRSRPLKG"
     gene            4840..6405
                     /locus_tag="VNP73_09080"
     CDS             4840..6405
                     /locus_tag="VNP73_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWL66112.1"
                     /translation="MKIRFALVVCLIAAALQPAAGANGRPEGCADPSHPGGEWRSYGH
                     DLSNTRTQPLEKKIDPSNAATLEPKWVFDTAAQDDVPDGGTFQNTPVVADGCMYTSTS
                     SGWAFALNADTGKVLWSTQLVGQGGGSLIGGVIVGSPTVADGMVFLAVSRPGSPYVAA
                     LDQDTGQVLWKSTVMTTKKFTAAFNATAVYYKGKIFQGWLGVEGSGPTRGGYAILDAS
                     RTCSDLPNVTCSEPRAGAKGGKILADEWTITDQEYKKGYHGASVWCSAALDPKEQYIY
                     ACGGNPHSEGKEARFANSLLKIDGDPSRKTFGQIVDHYKGRPDQYVSGADRQAVCDNF
                     GDETTVVVWSAGCLMLDLDFGASPNLFEDASGRLVVGDLQKAGVYHAVYADHMAPAWQ
                     TTVSAPCFSCNATSGAYANGRVFTVGSPPGHVVSIGARGQQEGRPQWVSPTHDGIHYQ
                     SVSTANGVVYVIDGYGNLNIVEAETGRPLMRRPIGVDVGQPAPDLASQGVAIARNRVY
                     AANGQWVVVYGLP"
     gene            6408..6863
                     /locus_tag="VNP73_09085"
     CDS             6408..6863
                     /locus_tag="VNP73_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66113.1"
                     /translation="MRTHRRGLAVVVVALIGLIAAPAGAAGTPVVMSQPASRETTYTT
                     PVVVIQPGEDVTFVNTDLFPHDVRSVLMGPDNTSWCKPADPDEPQHRVRNPRRFPIGK
                     CPLLWTPPIGMTNGVLESKLYGTDNVTSGTTVDFYCTVFPNMTGKVIVL"
     gene            6860..>7155
                     /locus_tag="VNP73_09090"
     CDS             6860..>7155
                     /locus_tag="VNP73_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66114.1"
                     /translation="MTRRLTILSLTCVMLFLMSVPTPAETTVPANVVTTGPGAQFYGY
                     VAPAVVTEKGGAITYVNLDIARHDFVQDVETDGIAGSQKKPWCKSYKKGTCPIF"
BASE COUNT         1362 a         2326 c         2138 g         1329 t
ORIGIN      
        1 atgaggctga gttcggcggt aggaaatcgg ctgcgggtca cgcccggatg ttagggaaga
       61 atgggttagg ccgagagctt ggcggggttc actttctcga cgtcggggga cctggtattg
      121 ccttcgatca ggtccaggac ggcctcctcg aacgcaacct ggatggcggg ctcgtcgaaa
      181 tcctcgtccg caagcttctc gatcacggga gcgatcgtca cgcccgagtc gtagcggtcg
      241 atcacctttg ggtcgacata agaagcccgg cagaccgcgg gggtgtttcc gagatagtga
      301 gaaacctcct ggatcgcacg cgagacggct ctcttgcgac ccgtcttcga gccaagtgcg
      361 ccacgcgaga ctgctaacgc gcgggcgcag agaaccgtgc cgtgccaagt cctgaagtcc
      421 ttggccgtgt aatcgccccc cgagatctcc ttgagatact cgttgatctc cgacgacttc
      481 acgtcgcgcc acctggaccc gagcttgtac gcgagcaact ccggcccccc cgaccgtcga
      541 cgcttgagag cgcgcaccac ctcgtagacc tccgggtcaa cgatcgattg gatgcgctcc
      601 ttgttgctct tggcgacgta ctcgaagacg acgcttctgc cctcgaaacg gacgtgatcc
      661 tttcggatcg tcgccaggcc ataggtctcg ttctcctccg cgtagtcctc gcctcccaca
      721 cgaaagaaac ctcggtcgag cagtcgtgct gcgcacgcaa gaacccgctc tcgaccgaga
      781 cccgaatggt cgagatgttc gttcgtgaag tgcctgatct tcgggagagc cttggcgaac
      841 tcgatcatgc gatcgaactt ctcctggtcc cgacgttgtc tccacgcggt gtggtacagg
      901 tactgcttcc gtcccgcggt gtcggttccg acagcctgta gatgaccgtt gtcgaacgga
      961 cagatccaga cgtcgatcca cgccggcggg atatgcagat cctttatccg ttgcagtacc
     1021 tgcagatccc cgatccgggt cccgtcgcta ttcagatatt cgaagccgcg accacgcttg
     1081 cggcgcgcgt agcccgcgga agcgcaatcg gaacgtctca accttggcat ggtggaccat
     1141 tcgtacccac ccttgctctg ctcgaaccgg gcccttaacc attcagctcc aataacgcga
     1201 cacggatacg agctgcgtcc tccggcggca cccagcgagc tatcgaatcg gcgatcgtct
     1261 ctgctgtgac gtcactatcg gttgcacgca aggcaagaac ggtcttgtgt acgaacgatc
     1321 ggtattcggc aggcaccttc acacctgcca tcgttccgga ctccaaccac tcttcctcca
     1381 gagcggccac ggccggatcg tctcgccact catcgatcgt cggtcctcgc ggtcccttac
     1441 gtaccgggat cacatttacc ggcttggaac cctcgcggcg ccagctcggc tgttcctggt
     1501 aatccggctc gaactcgatc cttcgacggg caaaagcctc gggatcgtcg tcatcgacga
     1561 ccaggacaag tcgaagggaa cggttgagct cgtcgggcac attccctgcg tcccggatgg
     1621 cgagagcttc atgcgtggac agccgtttga tcgtcgcaga ctcccgaccc gcgagaacag
     1681 cctcaacgtc ggccacgctc atgaggtacc aaccctcttc aagcatcccg gacttatagc
     1741 agtccgcgcc acgcggactt acttctcgat ctcgatgtcc tcgatcgttc cgtcgtcgtt
     1801 catctcgaac tcgagtactg cacccggatc gaggtcgtct tccgaggcct cggtctcgtc
     1861 gtcccagtcg atctcggtgt cgtcggtgac cgtcccggta accgtctctc ccgaatcgag
     1921 gcgaacggtc agcacgccgg agccggagtc gtacgaggtg atggtgccgt gctggtcgca
     1981 gtcctcatcg ccctcatcat cgccctcatc gtcgtcttcg tcttcgtcgt cgtcgtcgtc
     2041 gtcatcctcg acgcagtcga tggcgtcctc gccgtcggca tcctcggaga actcgtctcc
     2101 gtccgacgtg ccgtccccgt cgctgtcgct cagcttcggg tcggtcttga gttcgcggac
     2161 ctcttcgccg tccagcagac cgtcgtcgtc ggtatccggg tccaatgggt ccgtgccgtg
     2221 ccggtactca cccttgttct tgagtccgtc cgaatcgttg tccatgccgg agtcgtgcat
     2281 atgcgggttc gtcccgtgct tgctctccca cctgtgcggg atcccatccc cgtcgcggcc
     2341 gtgtgccgca gccggaaggc ccagcaaacc gaacgcgtaa tacgccgtca tggcgagtcc
     2401 gacggcgatc ctgatggccc ggatcgagaa aaccctcatt cgatcgctcc ttccgtgtag
     2461 acgagcaggt caagcggctg acacgcgccg aaccgaggct acgtagcggt tcctgaaaac
     2521 aacctgaacg gcggaggaat caccccttca cggccgaaat ccttactagg actggtagtt
     2581 cagtcgcgtc gagacgttgg gggcattcat ggcgaacaat gcgcgcaacc gcttcgcact
     2641 gcttggactt ctgctcggct tgcttgcgac gaccatcccg agcctgccgt cggcttcggc
     2701 cgccggccga gcggtcaaga tcagaactct gtccaaccgg gccgacttga tatccggagg
     2761 cgacgcgttg atccaggtga gcgttcccgc cggcgcgagc acgtccgaca tccgcctctc
     2821 gctcaacggc aaggacgtga ccggagcgtt ccaggaggga tcgaacggca acggccaggg
     2881 cctgatcgag ggactgaagc tgggacgcaa caaggtcgta gcttcgttgc cggacggacg
     2941 tgcggcgagc ctcacgatca cgaaccatcc cctcggtggc ccggtgttcg gcggcccgca
     3001 gatcaagccg tggacctgcc aggaagaggc tctcgacaag aagtgcaacc aggaaccgac
     3061 gatttcctac tactacaagc cggccggcgg cgggagcctg cagccttacg accgcgacaa
     3121 ccctccacca cccgaatcga tcgcggaaac gacgacgacc gaagaggtca cagttccatt
     3181 catcgtccgt gaagagatcg gctacatggc gcgcgatcag tacgcgatcg ccaccctttg
     3241 gcagccggac aagaagtggt cgccgtcgga accgcagaga cagttcaacc gcaggctcgt
     3301 gatcacgcat ggagccagct gcgacacctc ctacgaggtt ggagaagcac cggccgtcct
     3361 cgacgaggcg atcctcggtg ccggtttcat cgtgatgtcg cacgcgctcg acaacgccgg
     3421 ccacgcctgc aacctcctca tgcaagccga agcactggtc atgactaagg agtacgtgat
     3481 cgaccacttc gggaccgtcc gatggacgat cggcatgggg tgctcgggtg gctctttggt
     3541 ccagcagcaa gtggccaacg cctatcccgg gctgtatcag ggcatcacgc cacaatgcag
     3601 cttcaccgac gcgtggtcgt ccgccatgca gtacgtcgat tactacatgc tgctcgcgta
     3661 cttcaacgac cccagtcgct gggacccggg cacgtcgtgg tcccccgaca agatggccgc
     3721 ggtgctcgac catccgaaca tcggtaaccc ggtttcgttc acgagtgtca tcccgaacag
     3781 cggtaacccc agtcgttcat gtccgggtgt gaccgccgac aaggtctaca acgccgagac
     3841 gaacccagag ggtgttcgct gcacgctgca ggactacacg gtgaacgcct tcggacggcg
     3901 caagaaggac ggttttgcga accggccgtt cgacaacgtc gggatccagt acgggttgaa
     3961 aggacttcgc caaggcgtgc tcacccccgc cgagttcgtg gacctgaaca cacacctcgg
     4021 tggcctcgac atcgacggca acttccaagc gaaacgaagc aaagcggatc tcattggact
     4081 caagcgcgtc taccgcaccg gcttggtcga ctcggcgaac aacctcgaca aggtcgccat
     4141 catcgacctg cgtggaccgg atcccggcgc gttccacgac gtctatcgga cctatgcgat
     4201 gcgcgctcgc ctgttacgca acttcggcac ggctgcaaac caggtgctgt ggcgcggcca
     4261 ggtcgcgctg gtcggcgatc ccagcttcgc agtcgatgcc gtgttcgcgg tcgatcgatg
     4321 gttggcccgg gtcgacgcgg acgaccgcga cgtcccactc gcgcagaaga tcatcgagga
     4381 caaaccgagt gacatcgccg accgctgcac ggacggatcc ggtcgggagc aggtgtcgtg
     4441 ggtgtgcgac cagacggttg cctcttatgg cacgccgcgg ttcgcagcgg atgccccgta
     4501 taccgacgat gtgttgaagt gccgtcttca gcctttgcgg aaagacgact actcggtcga
     4561 gttcaccgat gagcagtgga aacgcctcca gaaggccttc ccggcaggcg tgtgcaacta
     4621 cgggaaaccg ggggtccacc agatgggggc gacgtcgtgg ctcacctatc aagatgctcg
     4681 aggccgggtg atctacggag gtcgtcctct cggaccgcct cctcgctccc gccctctcaa
     4741 ggggtagacc tgcagggatt cgggtagccg caacgaacgt ttcttgaggc ggccccacat
     4801 cgtcgtggag gcggccgccc ggccgatcta gggggtccga tgaagatccg tttcgcactc
     4861 gtggtgtgct tgattgccgc ggccttacag ccggcagcag gagcgaacgg tcgacctgag
     4921 ggctgcgccg atccctctca tcccggcggc gagtggcgca gctacggtca cgacctctcc
     4981 aacacacgca ctcagccact cgagaagaag atcgatccct ccaatgcagc gaccctcgag
     5041 ccgaaatggg tattcgatac ggctgctcaa gacgacgtac ccgacggagg cactttccag
     5101 aacactcccg tcgtggcaga cggctgtatg tacacctcca cgagctccgg ctgggcgttc
     5161 gcgctgaatg ccgacaccgg caaggtcctg tggtccaccc agttggtcgg acagggtggt
     5221 gggtcgctga tcggtggcgt gatcgtgggc tcgccgaccg tcgcagacgg gatggtcttc
     5281 ctcgccgtca gcaggccggg atcgccctac gttgccgccc tcgatcaaga cacgggccag
     5341 gttctgtgga agtcgaccgt gatgaccacg aagaagttca ccgcggcctt caacgctacg
     5401 gccgtgtact acaagggcaa gatctttcag ggatggctcg gagtcgaagg cagcggtccc
     5461 acgcgcggcg gctacgcgat cctcgatgca tcacgaacat gctctgacct tccgaacgtc
     5521 acctgctcgg aaccgagagc gggtgcaaag ggcggcaaga tcctcgccga cgagtggacg
     5581 atcaccgacc aggaatacaa gaagggctat cacggggcat cagtgtggtg ctcggcggcc
     5641 ctcgacccca aggagcaata catctacgcc tgtggaggca acccgcacag cgagggcaag
     5701 gaagcgcgct tcgcgaactc gctcctgaag atcgacggtg atccttccag gaagaccttc
     5761 ggacagatcg tcgaccacta caagggccgt ccggaccagt acgtgagtgg agccgatcgc
     5821 caagcggtct gcgacaactt cggggacgaa acaaccgtag tcgtgtggag cgccggttgt
     5881 ctgatgctcg atctcgactt cggcgcttcc cccaacctgt tcgaggatgc ctcgggtcgc
     5941 ctcgttgtcg gggacctaca gaaggctggt gtgtatcacg ccgtttacgc cgatcacatg
     6001 gcccctgcat ggcaaaccac ggtcagtgct ccctgcttct cctgcaacgc gacgtcgggt
     6061 gcatacgcca acggacgagt cttcacagtg ggaagcccac ccggacacgt ggtctcgatc
     6121 ggagctcggg ggcaacagga aggcaggccg caatgggtct cccccactca cgacggcatc
     6181 cactaccagt cggtctccac ggcaaacgga gtcgtgtacg tgatcgatgg ctacggaaac
     6241 ctgaacatcg tggaagccga aaccggccgg ccactgatgc gtcgaccgat cggggtcgac
     6301 gtcggacagc ccgcgccgga tctggccagt caaggtgttg cgatcgcgcg caaccgcgtg
     6361 tacgccgcca acggccagtg ggtcgtcgtc tacggattgc cctagagatg cgtacccatc
     6421 ggcgcggtct cgccgttgtc gtagttgctc tcatcggcct gatcgcggcc cccgccggcg
     6481 ccgccggaac cccggtggtc atgtcacagc cggccagccg cgaaaccact tacaccacgc
     6541 cggtcgtcgt catccagccg ggtgaggacg tgacctttgt caacacagat cttttcccac
     6601 acgacgtcag gtcggtcttg atggggccgg acaacacgtc gtggtgcaag ccggcggacc
     6661 ccgacgaacc tcagcaccgc gtccgcaacc cgcgccgatt cccgatcggg aagtgtccct
     6721 tgttgtggac gcctcctatc gggatgacca acggagtact cgagagcaag ctctacggaa
     6781 cagacaacgt gacttctgga acgacggtcg acttctactg caccgtgttc ccgaacatga
     6841 ccggaaaggt gatcgtccta tgacccgccg attgaccatc ctctcgctga cctgcgtgat
     6901 gctcttcttg atgagcgtcc ctacgccggc ggaaacaacc gtccccgcca acgttgttac
     6961 gaccggcccc ggcgcccagt tctacggcta cgtggctcca gccgtcgtaa cggaaaaggg
     7021 tggagcaatc acatacgtga acctggacat cgcacggcac gacttcgtgc aggatgtcga
     7081 gaccgatggg atcgccggct cgcagaagaa gccctggtgc aagagctaca agaagggcac
     7141 gtgcccgatc ttctg
//
LOCUS       DATMED010000352         4426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1038149, whole genome shotgun sequence.
ACCESSION   DATMED010000352 DATMED010000000
VERSION     DATMED010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038149"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VNP73_09095"
     CDS             <1..701
                     /locus_tag="VNP73_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWL66115.1"
                     /translation="GSFDDFNGVADVVEDRRAKGRPVWVHVDGAVGLLARASVEYQDL
                     VQGLDRLDSWSTDGHKLLNVAYDSGIAICRDASAHRAAMSVHASYLIHSDQETARDPM
                     DWNPEFSRRARGLGIYATLKSLGRSGVGEMVDQTCELARRFATGLEVTGKAEVVNDVV
                     FNQVLVRWLSDTGDHDAFNERVIDRVQHDGTAFFSGTTWNGMRLMRISVCDRATDEDD
                     VDRSIEALLHAASA"
     gene            complement(706..1647)
                     /locus_tag="VNP73_09100"
     CDS             complement(706..1647)
                     /locus_tag="VNP73_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWL66116.1"
                     /translation="MPDFKTVTVAAVQASPVILDADATVAKAIGLLHDAADGGAQLAV
                     FPETFVSVYPSGAWGHQAARFGGFDEMWERLWASSVEVPGPAVDKLVAACAERGIYCA
                     IGINEREIERPGSLYNSLLLLGPGGLLSKHRKLVPTMQERLFHGPGDGTDLDVVETDL
                     GRIGGLICWENRMPLARYELYSKGVQIWLAPTADDSDGWLATVRHIAIESGAFVISVP
                     QYIERSAFPDDFPVPLPDDEVFGRGGAAIIEPRGGEVIAGPLYDAEGIVTAEIDLKRG
                     LSAKRWFDVVGHYKRIDAGTSRSQEGGDWSPPETSIT"
     gene            complement(1693..3369)
                     /locus_tag="VNP73_09105"
     CDS             complement(1693..3369)
                     /locus_tag="VNP73_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HWL66117.1"
                     /translation="MASDADVIVVGAGIAGLVAAHELTSRGKKVALVDQENAANLGGQ
                     AYWSFGGLFLVDSPQQRRFGIKDSLELAWNDWRGSAQFDRLEDEDVWAVKWARAYVEW
                     AAGEKREWLESHGIDLLPTVGWAERGDLRADGHGNSVPRFHIAWGTGTGVVRPFAESA
                     QRAASEGLLQFHHRHRVDELVLTNGAATGVRGTVLADDDSDRGVASNRDETGEFELSA
                     QTVIVTTGGIGANHDIVRNYWPERLGTPPREMVTGVPAYVDGRMLDIAADAGARLVNR
                     DRMWHYTEGIQNWSPIWPGHGIRILPGPSSMWFDALGRRLPDPCLPGYDTLGTLQHLR
                     TTPDIVDFDHSWFILTQRIIEKEFALSGSEQNPDITNRDRKAFLKGRVLGKGAPPPVE
                     AFKTRGADFVVADDLDSLVAKMNALTEEATLDVTTIRAQIEARDLQIENPYSKDAQVQ
                     GIRNSRRFLGDRLSRTAKPHRILDAEAGPLIGVKLHILTRKTLGGIQTDLDSRAIGAD
                     GAPIPGLYAAGEVAGFGGGGVHGYNALEGTFLGGCLFGGRAAGRSAARDV"
     gene            complement(3385..>4426)
                     /locus_tag="VNP73_09110"
     CDS             complement(3385..>4426)
                     /locus_tag="VNP73_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66118.1"
                     /translation="ADLMDAWLDPHPAPGVYVPASDSTPAHYAIPGGPGLGEPDIYEH
                     VGLTAWHEWEVMGGRSPNFDQKLPKKLEVIERLRARYGDDLPIHIGYGIDPRIDGKRQ
                     NVRMAIEALVEDHVDHIVVAYHGVGYSDIMQTHMLRHEIHEILDEIAPEVTVSHTKPI
                     GTTDAYVRSVVRKARNEVAKLASDAKVAIHLSGHGLGTDMCGDYDCGADAYHDYARRL
                     FTRTKKALLRTVAHDGELGVFHVYGDGGSGDDDPEDEVDSPIEALTKRKEASFRFVID
                     IPYEFDSDSRDTLIILRRGYELPEGQWNENFETRFKWDGLRVKITNSSFGSGLKTDAY
                     EKVLVDALDNRQ"
BASE COUNT          811 a         1445 c         1341 g          829 t
ORIGIN      
        1 ccggatcctt cgatgacttc aacggagtcg cggacgttgt cgaggatcgg cgagcgaagg
       61 gtcggcccgt ctgggtccac gtcgatggag ccgttggtct attggctcga gcctcggtcg
      121 agtaccaaga cctcgtccaa ggcctcgatc gtctcgactc atggtcgacc gacggacaca
      181 agttgctgaa cgtcgcttac gactcgggca tcgctatctg ccgggatgca tctgcacatc
      241 gcgcagccat gagcgtgcat gccagttacc tcatccacag cgaccaggag acggcgcgcg
      301 atcccatgga ctggaacccc gagttctcgc gccgcgcccg cgggctcggg atctacgcaa
      361 cgttgaagtc actcgggcga agcggtgtcg gcgagatggt tgatcaaacg tgcgaactcg
      421 cccgtcgatt cgcaacgggg ctcgaagtca ccggtaaagc agaggtcgtt aacgatgtcg
      481 tcttcaatca ggtcctcgtc agatggctct ctgacaccgg ggaccacgac gccttcaacg
      541 agcgagtgat cgatcgggtc cagcacgacg gcacggcctt cttcagcgga acaacctgga
      601 acgggatgcg gctcatgcgg atctcggtct gcgatcgggc taccgatgaa gacgacgtcg
      661 accgttcgat cgaagccctg ctgcacgcgg cctcagcctg aaaacctagg tgatagacgt
      721 ctcaggcggc gaccaatctc cgccctcttg cgatcgggaa gtaccggcat ctatgcgctt
      781 gtagtgcccg accacatcga accacctctt cgcgctgagc cctctcttca gatcgatctc
      841 ggcggtaacg atcccctcgg cgtcgtaaag agggcccgcg atgacctcgc ctccccgagg
      901 ctcgatgatc gccgctcccc ctcgaccaaa gacctcgtcg tctggaagag gcactgggaa
      961 atcatccggg aaagcacttc gctcgatgta ttgcggaacg ctgatcacga aagctccaga
     1021 ctcgatcgcg atgtgacgca ccgtcgccaa ccacccgtcc gagtcatcag cggtgggcgc
     1081 gagccagatc tgaacgccct tcgaatacag ctcgtaccga gccagaggca tccggttctc
     1141 ccagcagata agtccaccga tcctgccgag atccgtctca accacatcga gatccgttcc
     1201 atccccggga ccatggaaca gacgctcctg catcgtcggg acaagcttgc gatgcttact
     1261 caatagaccg cccggcccca acagcagaag cgagttgtac agggaaccgg gccgttcgat
     1321 ctcacgttcg ttgatcccga tcgcgcagta gatgccgcgt tcggcgcatg ctgcaacgag
     1381 tttgtcgacg gcggggccgg gaacctcgac ggacgatgcc catagacgct cccacatctc
     1441 gtcaaagcca ccgaaccgcg cggcctggtg accccacgcg ccactcgggt acacggatac
     1501 gaatgtttcc ggaaagacgg cgagttgagc tcccccatcg gccgcatcgt ggagaagacc
     1561 gatcgccttg gctaccgttg cgtcggcatc gaggatgacg ggagaggcct gaaccgcggc
     1621 gaccgtgacg gttttgaagt cgggcacgag agtcacgtta ggcgagccga tcaagaccgg
     1681 tcaactggct cttcagacat cgcgtgctgc cgagcggccc gcggctcggc cgccgaaaag
     1741 acagcccccc aagaacgttc cttctagagc gttataccca tgaacgccgc cgcctccgaa
     1801 gccggcgacc tcgcctgctg catacaagcc ggggatcggc gctccgtccg ctccgatcgc
     1861 acgtgagtcc agatcggtct ggataccgcc caaggtcttg cgcgtaagga tgtgcaactt
     1921 caccccgatc aggggcccgg cctcggcatc gaggatccga tgcggcttcg ccgtgcggct
     1981 caggcggtcg ccgaggaagc ggcgggaatt acggatgccc tgcacctgcg cgtccttgct
     2041 gtacggattc tcgatctgca ggtcgcgcgc ttcgatctgt gcacggatcg tggtgacgtc
     2101 gagagtggcc tcttcggtca gcgcgttcat cttggcaaca agtgagtcga gatcgtccgc
     2161 taccacgaag tcggcgcccc gcgtcttgaa cgcctcgacc ggaggaggcg cgcccttccc
     2221 cagcacccgc cctttcaaga acgccttgcg gtcacggttc gtgatatcgg ggttctgttc
     2281 cgagcccgac agtgcgaact ccttctcgat gatccgctgg gtgaggatga accaagagtg
     2341 gtcgaagtcg acgatgtccg gcgttgtgcg cagatgctgg agcgtcccga gcgtgtcgta
     2401 tccgggcaga cacgggtccg gcagacggcg acccagcgcg tcgaaccaca tcgacgaggg
     2461 gcccggcagt atgcggatgc cgtgcccggg ccagatcggg ctccagttct ggatgccttc
     2521 ggtgtaatgc cacatccggt cccggtttac gagccttgcg ccggcatccg cggcgatgtc
     2581 gagcatgcgc ccgtcgacgt aggccgggac cccggtgacc atctcgcgcg gcggagtccc
     2641 gagtcgctcg ggccagtagt tgcgaacgat gtcgtggttc gcgccgatcc cgcctgtcgt
     2701 gacgatgacg gtctgcgcgg ataactcgaa ctcgccggtc tcgtcgcggt tgctggccac
     2761 accacgatcg gagtcgtcat ccgcgagaac cgttccgcgc acgccggttg cggcaccgtt
     2821 cgtgaggacg agctcgtcga cgcggtgacg atgatgaaac tgcaggagcc cttcgctcgc
     2881 cgcacgctgg gccgactcgg cgaacggccg gacgacgccg gtaccggtcc cccacgcgat
     2941 gtggaatcga ggaaccgagt tgccgtgtcc gtctgcacgc agatcgcccc gttccgccca
     3001 ccccaccgtg gggagcaggt cgatcccgtg ggattcaagc cactcacgct tctcacccgc
     3061 agcccactcc acgtaagcgc gtgcccactt gaccgcccaa acgtcttcgt cctcgaggcg
     3121 atcgaactgc gcgctccccc gccagtcgtt ccatgcgagc tcgagggagt ctttgatgcc
     3181 gaatcgacgc tgctggggtg aatcgaccaa gaacaaaccg ccgaatgacc agtacgcctg
     3241 gccgccgagg ttcgcggcgt tctcctgatc gaccagcgcg accttcttgc ctctagaggt
     3301 cagttcgtgg gcggcaacga gtccggcgat gccggccccc acgacgatca cgtcggcgtc
     3361 gctcgccacc ctcgcctcct cggatcattg cctgttgtcg agcgcgtcaa cgagcacttt
     3421 ctcatacgcg tccgtcttca gccccgagcc gaacgagctg ttagtgatct tcacgcgcaa
     3481 tccatcccac ttaaaccgag tctcgaagtt ctcgttccac tggccctccg gcaactcgta
     3541 gccgcgacgc aggatgatca gtgtgtcgcg ggagtcggaa tcgaactcgt aggggatgtc
     3601 gatcacgaaa cggaagctcg cttccttgcg tttcgtcagg gcctcgatcg gggagtccac
     3661 ctcgtcctcc ggatcgtcgt cgcccgaccc tccatcgcca taaacgtgga acacgccgag
     3721 ctccccgtcg tgggcgaccg tccgcagaag ggctttcttc gtacgagtga atagacggcg
     3781 ggcgtagtcg tgatacgcat cagcaccaca gtcgtaatca ccgcacatgt cggttcccaa
     3841 cccatggccg gatagatgga tggcaacctt cgcgtccgag gccagcttcg ccacttcgtt
     3901 tcgagccttg cggactacag atcgcacgta agcatcggtg gtgccgatcg gtttcgtgtg
     3961 ggagacggtc acttctggag ctatctcgtc gaggatctcg tggatctcgt ggcgcagcat
     4021 gtgcgtttgc atgatgtcgg agtacccgac gccgtggtac gcaacgacga tgtggtcgac
     4081 gtggtcttcg accaaggcct cgatcgccat cctgacgttc tgacgcttcc cgtcgatgcg
     4141 cggatcgatg ccgtagccga tgtgtattgg aaggtcgtcg ccgtaacgag cccggaggcg
     4201 ctctatcact tcgagcttct tgggaagctt ctggtcgaag ttgggcgatc gccctcccat
     4261 gacctcccac tcatgccatg cggtcagccc gacgtgttcg tagatatcgg gctctcccag
     4321 acccggccct ccggggatcg cgtaatgagc cggggtgctg tcggaagccg gaacgtagac
     4381 acccggcgcc gggtgtggat cgagccacgc gtccatcaga tcggct
//
LOCUS       DATMED010000353         3716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4129966, whole genome shotgun sequence.
ACCESSION   DATMED010000353 DATMED010000000
VERSION     DATMED010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4129966"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VNP73_09115"
     CDS             <1..954
                     /locus_tag="VNP73_09115"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HWL66119.1"
                     /translation="NPNGTVTLVKTTTLAGYRYGVEHYITVFKFDGGGAKFGSIVPLP
                     AIPSKIRRGGDWTLQRLVREVAPPPAPNEAQPSGGRAEDDAKVVKEKVIDSVRVQILK
                     GGGDAVGRWAKNEGFALPPDAPEVLDFYAKRSQIFMALRFIPKEAEESGKTNGDGVPI
                     HLNIRTDQPWVPLRILGLGQTSSTFVEADVFLLTKRQPALLPKPAKAGSGEGMILERD
                     ELASQSLLDDLRSDKGGMEWVHPKNMWLTYLRLNLEAGDLTYDLATNIRGGQPNADDT
                     GEGSRKDGGSTPQSPIWMWGAASLFGVGSLALTNKVLSHKD"
     gene            1020..2054
                     /locus_tag="VNP73_09120"
     CDS             1020..2054
                     /locus_tag="VNP73_09120"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HWL66120.1"
                     /translation="MRRIVCAFAVAIAFCVMGAAPAWACGGLVNPNGTVSLVKTTTLA
                     GYRYGVEHYLTSFKFQGGGAKFGSIVPLPGVPSKVRRGGDWTLQRLVREITPPPPPRP
                     TPDGTFTTTATTTSAAQVLYQTEIDDVQITILRGGGIAVGKWAKKNGFTLTPDAPEVL
                     DYYAKKSPIFMAASFDPQEAQGSGKQIGDGVPIHLNIRTDNPWVPLRILALGQADVAQ
                     VEADVFLLTKREPSLAPTPESALASRGLILKRSEQASPSLLTDLRSDKGMQWVHDSTM
                     WLSYLKVDVDAKDLTYDLAADVKGMAMGIPPKEESPPREEAVVWSWGAASAIGLGTIA
                     VTNVLFGNRS"
     gene            2054..2458
                     /locus_tag="VNP73_09125"
     CDS             2054..2458
                     /locus_tag="VNP73_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWL66121.1"
                     /translation="MRGVVLFLGVLGLLGACAGSPGPPQGTEEVSVTIEHSRFEPDEM
                     EFRAGQTVRFVIRNTDPIDHEFILGDEEIQQIHEEGTEAHHGAKPGEISIPAGTTRTT
                     MFTFSDEGDLIYGCHLPGHYDFGMKGTVSVSS"
     gene            2472..2945
                     /locus_tag="VNP73_09130"
     CDS             2472..2945
                     /locus_tag="VNP73_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWL66122.1"
                     /translation="MGFDLLSIQPDTQAEILVRVALAALLGGIVGFEREFSDQPAGLR
                     THLLVSLGAALFSMAGAFAGTDPTRIAAQVAAGIGFIGAGVIFQQGLNVRGLTTAAAL
                     WVTAAIGTAVAFGLWVAATGSTIITVVALYGLKRFELWIFGHRKAEGDDSEDEGG"
     gene            3044..>3716
                     /gene="selD"
                     /locus_tag="VNP73_09135"
     CDS             3044..>3716
                     /gene="selD"
                     /locus_tag="VNP73_09135"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWL66123.1"
                     /translation="MKTTSQDPALLVGLDAPDDAAVYRIAPDNALVQTVDFFTPVVDD
                     AFAWGRIAAANAMSDVWAMGGRPALALNLVGWPRSLGFELLGRVLDGAAEACSEAGVA
                     IVGGHSVDDPEPKFGLAVTGFVHPDRVVRKRGGVPGDDLILTKPLGMGIVSSGIKDGK
                     TSDETGQEAMRIMGTLNKGAAEVMVEVGVHAATDVTGFGLIGHLLEMLDGHVAAELEV
                     NAIPFL"
BASE COUNT          709 a         1089 c         1209 g          709 t
ORIGIN      
        1 aacccgaacg gcaccgtaac cctggtcaag acgaccacgc tcgcggggta ccgctacggg
       61 gtcgagcact acatcacggt gttcaagttc gacggcggag gcgcgaagtt cggctcgatc
      121 gttcctctcc cggctatacc cagcaagatc cgtcgcggcg gtgactggac ccttcaacgc
      181 ctggtgcgcg aggtcgcgcc cccgcctgca cccaacgaag ctcaaccgtc gggtggccgt
      241 gccgaggacg acgcaaaggt agtcaaggag aaagtgatcg actcggtgcg tgttcagatc
      301 ttgaagggtg gcggcgacgc cgtcggacgc tgggccaaga acgaaggctt cgcgttgcct
      361 ccggacgctc ccgaagtact cgacttctac gcgaaacgga gccagatctt catggcgctc
      421 cgtttcatcc cgaaagaggc tgaagagagc ggcaagacca atggtgacgg tgttccgatc
      481 catctgaaca tccgcaccga tcaaccgtgg gttccgctgc ggatcctcgg gctcggacag
      541 acctcttcca cgtttgtcga ggccgacgtc ttcctgttga ccaaacgcca gcctgcccta
      601 cttccgaagc ctgccaaggc cggatcgggc gaaggcatga tcctggaacg cgacgaactc
      661 gcttcgcagt ctcttctcga cgaccttcgt tccgataaag gtgggatgga gtgggtgcat
      721 ccgaagaaca tgtggctgac ttatctgcga ctgaacctcg aggccggaga tctgacgtac
      781 gacctcgcga cgaacatccg tgggggccag cccaacgcgg atgacaccgg cgaaggctcg
      841 aggaaggacg gtggatcgac gccgcagagc ccgatctgga tgtggggtgc cgcgtcgctg
      901 ttcggtgtcg gatctctcgc gctgacgaac aaggtgctgt cgcacaaaga ctgaagcctt
      961 agaacttttc tcgtaacccg tcgttgtagt tgataagtgg gttgcgagag ggggacaaag
     1021 tgcgaaggat cgtctgtgcg tttgccgttg caatcgcgtt ctgtgtcatg ggagcggcac
     1081 cggcgtgggc atgtggcgga ctcgtcaatc cgaacggcac ggtgagtctc gttaagacca
     1141 cgacgctcgc cggttaccgg tatggcgtgg agcactactt gacctccttc aagttccagg
     1201 gtggcggtgc aaagttcggt tcgatcgttc cgctccccgg tgttccatcc aaggtccgtc
     1261 gcggaggtga ctggaccttg cagcgtttgg tgagggagat cactcctcca ccgccacccc
     1321 gtccgacgcc ggatggaaca ttcaccacaa cggcgactac gacgtcggcg gcccaagtgc
     1381 tgtaccagac cgagatcgac gacgttcaga tcacgatcct gcgtggaggt gggatagccg
     1441 tcgggaagtg ggccaagaag aacggtttca cactcacgcc cgacgccccg gaggtactcg
     1501 actactacgc gaagaaaagc ccgatcttca tggcggcaag cttcgaccca caggaggcac
     1561 agggcagcgg caagcagatc ggcgacggcg tcccaataca cctcaacatc cgcacagaca
     1621 atccttgggt gcctttgcgc atccttgcgc tcggtcaagc agacgttgcc caagtggaag
     1681 cggacgtgtt cctcctgacc aagcgtgagc cgagtctcgc tccaactccg gagtcggctc
     1741 tcgccagtcg cgggttgatc ctcaagagaa gcgaacaggc ttcgccctcg ctgctcaccg
     1801 atctgaggtc cgacaagggc atgcagtggg tacacgattc gacgatgtgg ctgtcctacc
     1861 tgaaggtcga cgtcgacgcc aaggacctga cctatgacct tgccgccgac gtgaagggga
     1921 tggcgatggg tatacctccc aaggaagaga gtccgcctcg ggaagaggcc gtcgtctgga
     1981 gctggggtgc tgcatctgcg atcggattgg ggactatcgc cgtgacaaac gttctgttcg
     2041 gtaacaggag ctgatgcgag gggtcgtgct cttcctcggc gtgctgggac tgctcggtgc
     2101 atgcgccggg agtcccggcc cgccgcaagg aaccgaagag gtaagtgtca cgatcgagca
     2161 ctcgcgcttc gaacccgacg agatggagtt ccgggccggg cagaccgtca ggttcgtgat
     2221 ccgcaatacg gatccgatcg atcacgaatt catccttggt gacgaggaga tccagcagat
     2281 ccacgaggaa ggcaccgaag cccatcatgg tgccaagccc ggtgagatct cgatcccggc
     2341 gggcacgacc aggaccacca tgttcacatt ctccgacgag ggagacctga tctacggctg
     2401 ccacctgccg gggcactacg acttcggcat gaaggggacg gtttcggttt cctcctgact
     2461 atcctgggcg cgtgggattc gacctcttat ccatccagcc cgatacgcaa gcagagatct
     2521 tggttcgcgt ggctcttgca gctctcctgg gagggatcgt gggattcgaa cgagagttct
     2581 ctgatcagcc tgccggtttg cgaacccatc tacttgtctc tctgggggcc gcgcttttct
     2641 cgatggccgg agcattcgcc gggacggacc ccactcgtat cgcggctcag gttgcggcgg
     2701 ggatcggttt catcggtgcc ggcgtcatct tccagcaagg tttgaacgtc cgaggtctaa
     2761 cgacggccgc ggcactttgg gtcaccgcag cgatcggaac ggccgttgcc ttcggattgt
     2821 gggttgcagc taccggctcg acgatcatca ccgtggtagc cctctacggg ttgaagcgct
     2881 tcgaactctg gatcttcggc caccgcaaag ccgaaggaga cgattcggag gacgaagggg
     2941 ggtgaccgcg ttgtccagcg agattcgact cacgtcctat tcgcacgggg gcggctgagc
     3001 ctgcaagctc ggaaccaccg agttggcgca ggttctgcgt cacgtgaaga ccacttccca
     3061 ggatccggcc ttgctggtcg gactggacgc gcccgacgat gcggccgttt atcggatcgc
     3121 tccggataac gcgctcgtcc agacggtcga ctttttcact ccggttgtcg acgatgcgtt
     3181 cgcgtgggga cgcatagccg cggcgaacgc gatgtcggat gtgtgggcga tgggcgggag
     3241 acccgcgttg gcactcaacc tggtcggatg gccccggtcg ctgggattcg aacttctcgg
     3301 tcgtgtctta gatggagccg cggaggcgtg ttcggaggcc ggcgtcgcga tcgtgggggg
     3361 ccattcggtc gacgaccccg agccgaagtt cgggctcgcg gttaccggct tcgttcaccc
     3421 ggaccgagtc gtgcgcaaga ggggaggggt cccgggggac gatctgatac tcaccaaacc
     3481 tctggggatg gggatcgtgt cgagcgggat caaggacgga aagacgtccg acgaaactgg
     3541 gcaagaggcg atgcgcatca tgggcacgct caacaagggg gccgctgagg tgatggtgga
     3601 ggtcggcgtt cacgcggcga ccgacgtgac cgggttcggc ttgatcggtc atcttctgga
     3661 gatgctcgat ggacatgtgg ccgcggagct cgaggtcaac gcgatcccct tccttg
//
LOCUS       DATMED010000354         6011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1040163, whole genome shotgun sequence.
ACCESSION   DATMED010000354 DATMED010000000
VERSION     DATMED010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040163"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1077
                     /locus_tag="VNP73_09140"
     CDS             121..1077
                     /locus_tag="VNP73_09140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWL66124.1"
                     /translation="MEDTSQRAPSAFVIATRNRPGDLLKTVQSLVEQTVLPGELCIVD
                     SSDDTPTRAEIEALCDKVGIPLDFHHPAPRGLTVQRNVGIDRTKGDPVFFIDDDVWLA
                     PDCHEQVLAEYERWGEAVGGVRATPVRPARPSLSTRLYRKLFGIGGWWPENSGRIRPG
                     FYAEGISESVGSRKVEYFNGWFMSYRREVFAHERFDEALSGYAYKEDIDFSYRVFLRG
                     HTLVQTPKARCDHLKSDASRLNSHQLQRMNLANQFYLHRKLIPQTFAHKAALWWALTG
                     LFILNVGKALQTNDWGLVTGLVAGASEQARGRGLVDPALERS"
     gene            1074..1736
                     /locus_tag="VNP73_09145"
     CDS             1074..1736
                     /locus_tag="VNP73_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWL66125.1"
                     /translation="MTFEADERGAKKRDYWWTVLFTDPIAIPLSRFLAKKRWLTPDQV
                     TIVALILGILVGVLFATGERWGLIAGGVTFYAAFIVDCVDGKLARALGVTSARGEHLD
                     ALADGGRRASASLGIGILLWGEDPILWVLIYVVLAYYFLEISGAEKGQSGSGLGGKWS
                     QALAKRRLLPNPGMPDVQAIVFILGPITGLVVPALWLGIAMVTVAILMTIRRRWAAGG
                     RS"
     gene            1778..3394
                     /locus_tag="VNP73_09150"
     CDS             1778..3394
                     /locus_tag="VNP73_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL66126.1"
                     /translation="MSKVLMLGLDGMTYNVLEPAFAAGHMPAFKALMDRSASGVLTST
                     VPPYTPPGWTSIFTGVNPGKHGIFGFTLGNVQRRGGLVRLDRVKAPAIWNVANAQGMR
                     MGVFNVPMTYPAPVVEGWSVSGMLTPEGGGETPDNFTYPEHLAKGIVAAAGSYEIDIE
                     VNYDEDWKSTAIIERLSRNLSLKRRALQHLLATDGDLPLLFGVLEAPDRLMHVHYKYI
                     DPRCEHYNRPEAAPIRERAWAFFDEMDAVIADMLAWAGDDAYVLTMSDHGFGPKEKSV
                     NVNVALREWGLLDVGGAGAVTKSAGVRKVARRVKKALPKSVWQRAKGAAQSSIDWSKT
                     KAFSAPIPQQGIYVNLAGREPNGIVSQSEYESVRDEIIERFQALIDPDDGKPVLDRIY
                     KREEVVHGPVAEDAPDLFPVCRGYTYELSDGTYSPSVLDDYRELPRGFHQMDGIFGIA
                     GPDAIPANGLEASIYDIAPTALYIAGCKLPRMDGRVLLEYLPESLTDGRPVVEEDMDL
                     PLAGAGAEASPYSAEEEAQIEESLRNLGYL"
     gene            3391..4695
                     /locus_tag="VNP73_09155"
     CDS             3391..4695
                     /locus_tag="VNP73_09155"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL66127.1"
                     /translation="MRKLVAAIALLLVVAGCDDPTVPAAKPSAAQARGIPIEKVVFIV
                     KENRTFDNMFGRFPGADGARYGRLSNGQRVRLTRGPDVYPHDIAHGFFDGLVVVNGGK
                     MNGFDLIAGGKNDRLPYTQYAREDLPNYWRYANRFALADRFFSSMYGPTIPEHLFTMA
                     ATGGRVVSNKLTPEDGKGLYCEDVRERFHKLMKHRRLIHWERTVQLDKIEALLREIRA
                     CLDVKTIFPELDKKGVSWRYYGKRDQFHNALLAVREVRFTKRWKNVGHPEDFVKDARS
                     GDLPQVSYILPPKKYNEHPHPKGRSVCIGENWTVRQVNAVMQGPDWERTAIFVTWDDF
                     GGLYDHVNPPVIDDMGLGPRVPLLIISPWVKPSSIPHATYEFSSFLAFLERLHGIEPL
                     TRRDRIANDMFSMFDFDQEPLDPLILETRPERPGAKPPRCRL"
     gene            4845..5225
                     /locus_tag="VNP73_09160"
     CDS             4845..5225
                     /locus_tag="VNP73_09160"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HWL66128.1"
                     /translation="MARSYAKVVGVVVLLVGVVGLIMGDPEDGLLGFNIDLVEDIVHL
                     ATGGLLAYVGFAGGDAQAKSIVMIIGVVYLLVGVLGFVLPGLFGLLPTELAVQDNLLH
                     LVLGALGIWAARGAAPAATPGATA"
     gene            complement(5284..5664)
                     /locus_tag="VNP73_09165"
     CDS             complement(5284..5664)
                     /locus_tag="VNP73_09165"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding ACT domain-containing
                     protein"
                     /protein_id="HWL66129.1"
                     /translation="MLEEITVTADSEEPGALAQIGELLGAAGVNIETLSAFAFNGTGV
                     VHLVVDDGDDAAEVLRKNGLRVESSRPILTATLDDRPGELGRFCRKLAKAGVDIEAAY
                     IVRRGGGETELIFAVDDLEAAQDA"
     gene            complement(5706..>6011)
                     /locus_tag="VNP73_09170"
     CDS             complement(5706..>6011)
                     /locus_tag="VNP73_09170"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWL66130.1"
                     /translation="LRRFSLDELPQLWNVLKGEMSLVGPRPALPTEVEQYDDWVRNRL
                     KVKPGMTGLWQVSGRTETSFSDYVRYDLFYIQNWSTSLDLWILWRTFRAVTSAEGAH"
BASE COUNT         1092 a         1852 c         1967 g         1100 t
ORIGIN      
        1 aagaggagca cagccaggat gagggccggg aatcgtcggc gccgcttgtg aaacaacgca
       61 ggcgaacgcg cagacggttt gaatgccgcc acggggccaa acggtaccct gctcggcgtg
      121 atggaagaca cgagccagcg cgccccgtcg gcgttcgtca tcgccaccag gaataggccc
      181 ggagacctgc tgaaaacggt ccagagcctg gtcgagcaga ccgttctgcc gggcgagctg
      241 tgcatcgtcg attcctccga cgacaccccc accagagccg agatagaagc tctgtgcgac
      301 aaggtgggca tccccctcga cttccaccat ccggccccca ggggcttaac cgtccaacga
      361 aacgtcggta tcgacaggac gaagggggac cccgtcttct tcatcgatga cgacgtgtgg
      421 ctcgcccccg actgtcacga acaggtgctg gcggagtacg agcgctgggg ggaggccgtg
      481 ggcggtgtgc gcgcaacgcc ggtgcgtccg gcgcggccgt cgctgtcgac gcggctttac
      541 cgcaagctat tcgggatcgg cggatggtgg ccggagaaca gcggacggat ccgtcccggt
      601 ttctacgccg aggggatctc ggaatccgtc ggtagtcgga aggttgagta cttcaacggc
      661 tggttcatgt cctatcggag agaagtgttc gcgcacgagc gcttcgacga ggcactctcc
      721 ggctacgcgt acaaggagga catcgacttc tcgtatcgcg tgttcctgcg cggtcacacg
      781 ttggtacaga caccgaaggc tcgctgcgat catctgaaga gcgacgcgtc ccggttgaac
      841 tcgcatcagc tgcagaggat gaacctcgcg aaccagttct acctgcatcg caaattgatc
      901 ccacagacat tcgcgcacaa ggcggcgttg tggtgggcgt taaccggcct cttcatcctg
      961 aacgtcggta aagcattgca gaccaacgat tggggcctcg tgaccggtct ggtcgcggga
     1021 gcgagcgagc aggcccgcgg gcggggcctc gtcgatccgg ccctggaacg ttcatgacct
     1081 tcgaagccga cgagcgcggt gccaagaagc gcgactactg gtggacggtt ctattcaccg
     1141 acccgatcgc gatcccgctg tcacgtttcc tggcgaagaa gcgctggctc acgcccgacc
     1201 aggtgacgat cgtggccctg atcctgggca tcttggtcgg cgtgctcttc gccaccggtg
     1261 agcggtgggg gctgatcgcc ggcggtgtga ccttctacgc ggcattcatc gtcgattgcg
     1321 tggacgggaa actcgcccgg gcgctggggg ttactagcgc tcgcggggag cacctcgatg
     1381 ccctcgccga tggaggtcgc cgggcgagcg catcgttggg catcggcatc cttttgtggg
     1441 gagaggaccc gatcctgtgg gtcctgatct acgtcgtgct tgcctattac ttcctcgaga
     1501 tctcgggggc cgagaaaggg cagtccggct cggggctggg gggaaagtgg tcccaggccc
     1561 tcgccaagcg ccggctgctg cccaacccgg ggatgcccga cgtccaggcg atcgtgttca
     1621 tcctcggccc gatcacgggc ctggtcgtcc cggccctgtg gctcgggatc gccatggtca
     1681 ccgttgccat cctcatgacc atcagaaggc gttgggcagc gggcgggcgg tcctgaatga
     1741 gcgggcgaag cccgctccgc cttagggtgc ccgcgtcatg agcaaggtcc tgatgctcgg
     1801 cctcgatggg atgacctaca acgtgctcga gccggcgttt gccgccggcc acatgcccgc
     1861 gttcaaagcc ttgatggatc ggagcgcatc gggggtcctg acctcgacgg tcccgcccta
     1921 caccccgccc ggatggacct cgatcttcac cggggtgaac ccgggcaagc acgggatctt
     1981 tggattcacc ctgggcaacg tgcagcgtcg gggcgggttg gtgcgtctag accgggtgaa
     2041 ggcaccggcg atctggaacg tggcgaatgc tcaagggatg cggatggggg ttttcaacgt
     2101 gccgatgacg tacccggccc ccgtggtcga gggatggtcg gtgtccggga tgttgacgcc
     2161 cgagggcggg ggcgagacgc cggacaactt cacctacccc gaacatctgg cgaaagggat
     2221 cgtggccgct gccggttctt acgagatcga tatcgaggtc aactacgacg aagactggaa
     2281 gtcgaccgcg atcatcgagc gcctgtcgcg caacttgagc ctcaagcgcc gcgctctgca
     2341 gcacctcctc gccaccgacg gcgatctccc actgttgttc ggcgtcctgg aagcgccgga
     2401 cagactcatg cacgtccact acaagtacat cgacccgcgt tgcgagcatt acaaccgtcc
     2461 cgaggccgcc ccgatccgcg agcgggcgtg ggcgttcttc gacgagatgg acgcagtgat
     2521 cgccgacatg ctggcgtggg ccggcgacga cgcgtacgtc ttgacgatgt ccgaccacgg
     2581 tttcggacca aaggagaaga gcgtcaacgt gaacgtcgcc ctgcgcgagt ggggcttgct
     2641 cgacgtcggg ggtgcgggtg cggttacgaa gtcggccggt gttcgcaaag tcgcacggcg
     2701 ggtcaagaaa gcgctcccga agtccgtatg gcaacgggcg aagggtgcgg cgcagtcttc
     2761 gatcgactgg tcgaagacga aggcgttttc ggcccccatc ccgcagcagg ggatctacgt
     2821 gaacctcgcc ggccgcgagc cgaacggcat cgtttcccag tccgagtacg agtcggtgcg
     2881 agacgagatc atcgaacgct tccaggcttt gatcgacccc gacgatggca agccggtgct
     2941 cgaccgcatc tacaagcgcg aagaggttgt gcatggcccc gtagcggagg atgcccccga
     3001 tctgttcccg gtgtgccggg gatacacgta cgagttgtcc gacggcacct actcgccctc
     3061 ggtgctcgac gactaccgcg agttgccgcg cggcttccac cagatggacg gcatcttcgg
     3121 catcgccggc cccgatgcga tacccgcgaa cggtctggag gcgtcgatct acgacatcgc
     3181 accgaccgcc ctgtacatcg cggggtgcaa actgccgcgg atggacggcc gggttctgct
     3241 cgagtaccta cccgagtcgc tgacggacgg acggccggtc gtggaagaag acatggatct
     3301 tccgctggcc ggcgcggggg ccgaggcgag tccatactcg gccgaggagg aagcccagat
     3361 cgaggagtct ctgaggaatc ttggttacct gtgaggaaac tggtcgcagc gatcgccctt
     3421 ctacttgtcg tcgccggatg cgacgacccc actgttccgg cggcgaagcc ttccgctgcg
     3481 caagcgcgcg gcatcccgat cgagaaggtc gtcttcatcg tcaaggagaa ccgcaccttc
     3541 gacaacatgt tcggccgttt cccgggagcc gacggggctc gttacggacg cctttcgaac
     3601 gggcaaaggg ttcgcctgac acgcggcccc gacgtttatc cccacgacat cgcgcatggg
     3661 ttcttcgacg gactcgtcgt ggtcaacggc gggaagatga acgggttcga tctgatcgcc
     3721 ggcggcaaga atgatcggct tccgtacacg cagtacgcgc gcgaggatct cccgaactac
     3781 tggcgttacg ccaataggtt tgccctcgcc gaccgcttct tctcgtcgat gtacggacct
     3841 accatcccgg agcatctatt caccatggct gcgacgggtg gccgggtggt ttcgaacaag
     3901 ctgaccccgg aggacggcaa ggggctctat tgcgaggatg tgcgtgagcg gttccacaag
     3961 ctcatgaagc accggcggct gatccactgg gagcgcacgg tccagttaga caagatcgag
     4021 gccctgctca gggagatccg ggcttgtctc gacgtgaaga cgatcttccc ggagctcgac
     4081 aagaaggggg tttcgtggcg ctactacggg aagcgtgacc agttccacaa cgcccttctc
     4141 gcggtgcggg aggttcgctt caccaagagg tggaagaacg tcggtcaccc ggaagacttc
     4201 gtcaaggatg cacgtagcgg cgatcttcct caggtgtcgt acatcctgcc acccaagaaa
     4261 tacaacgagc acccgcaccc caaaggccga agcgtctgca tcggtgagaa ctggacggtg
     4321 cggcaggtca acgcggtgat gcaaggcccg gactgggaac gaactgcgat cttcgtgacg
     4381 tgggatgact tcggtggcct ctacgatcac gtgaacccgc cggtgatcga cgacatgggg
     4441 ctgggtcccc gggtcccgtt gctgatcatc tcgccgtggg tgaaaccgag ttccatcccc
     4501 cacgcgacct atgagttctc gtcgttcctc gcgttcctcg agcggctcca tgggatcgaa
     4561 ccgttgacta ggcgcgatcg catcgcgaac gacatgttca gcatgttcga cttcgaccag
     4621 gagccgctgg accccctgat attggaaacc cggccggaac gcccgggcgc caagccccct
     4681 aggtgccgcc tgtaaccgca ggtgccgtag cggcgtcgga ccacccggca aaccggcgat
     4741 agccgggggc ccaatccggg caactgtggc ctttcgaata cacaatcgga aggtgacgaa
     4801 aggctcagga cccgggcctt agcacgttag ggaaggggtg cgcagtggct aggagttacg
     4861 caaaggtcgt cggggttgta gtgcttctcg tcggggtcgt ggggttgatc atgggtgacc
     4921 ccgaggatgg gcttctcggt ttcaacatcg acttggtcga ggacatcgtg caccttgcta
     4981 ccggtggcct cctcgcatac gtcgggttcg cgggaggcga tgcccaggcg aagtcgatag
     5041 tcatgatcat cggagtcgtc tatctgctcg taggcgttct tggtttcgtg ttgcccgggc
     5101 tcttcggtct ccttccgacc gagctcgcgg tacaggacaa cctcctgcac ctggtgctcg
     5161 gagcgctcgg catctgggcc gctcgcggag cggcaccggc cgcgactccc ggcgctaccg
     5221 cttagtagct gaaagagaga aaggccccgc ctttaggagg cggggccttt cttttggtct
     5281 cgtctaagcg tcctgagccg cctcgagatc gtcgacggcg aagatcaact cggtttcccc
     5341 gccgccgcgt cgaacgatgt aggcagcttc gatgtccacg ccggccttag cgagcttgcg
     5401 acagaagcga cccagttcac ccgggcgatc gtcgagagtt gcggtgagga tcgggcgaga
     5461 gctttcaacc ctgagcccat tcttgcgaag gacctcggcg gcatcgtcgc cgtcgtcgac
     5521 gacgagatgc acgactccgg ttccgttgaa ggcgaacgca gatagcgttt cgatattgac
     5581 gccggccgct cccagcaact cgccgatctg tgcgagtgct ccgggctcct ccgaatcggc
     5641 ggtgacggtg atctcctcga gcatgatcga agcgtactcc tttgcccagc cgaccggcag
     5701 tggctttagt gcgctccctc cgcgctggtc acggcgcgga acgttcgcca caagatccag
     5761 aggtcaagag aggtggacca gttctggatg taaaaaaggt cgtaacggac gtagtcggaa
     5821 aaactcgtct cggttctccc gctcacctgc cacagaccag tcatccccgg cttcaccttc
     5881 aggcggttgc ggacccagtc gtcgtactgc tcgacttcgg tgggaagggc gggacgcgga
     5941 cccaccaagc tcatctctcc tttgagcacg ttccacagct gagggagctc gtcgagcgag
     6001 aaccgcctca g
//
LOCUS       DATMED010000355         7237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_673156, whole genome shotgun sequence.
ACCESSION   DATMED010000355 DATMED010000000
VERSION     DATMED010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673156"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNP73_09175"
     CDS             complement(<1..189)
                     /locus_tag="VNP73_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66131.1"
                     /translation="MEREYDFAAIEQRWMKEWIEGKYWAAPEIPDRSKKFYALTMYPY
                     PSGDMHMGHVEIFSIHDSI"
     gene            complement(232..1005)
                     /locus_tag="VNP73_09180"
     CDS             complement(232..1005)
                     /locus_tag="VNP73_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66132.1"
                     /translation="MRYARCSRFPVQILAAAMVIAMAGCDNTPDTQPEGAASSPRARA
                     NLDCTDEQQVIEDVSLGSGGSVEGDVDGDGEIDTVQIVLDENGEPGCEAFLIVGTSSG
                     NVSIPIWLTGSTGGLPQPSIKTLADVDAKPGLEIVVDEAGGASTQFAGVYTLVDGDLA
                     WIELPESEMGLFAYGGSVGHLEAVDCAGDEELFVTRAVPGQGNEALEKSLYDVTRTTY
                     SIDGGTLEQVSTETDQVPIDRLDRFKEFQTSPFGTCSSG"
     gene            1047..2027
                     /locus_tag="VNP73_09185"
     CDS             1047..2027
                     /locus_tag="VNP73_09185"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HWL66133.1"
                     /translation="MIGSKRVGAAVLVLVLVFVLGACSGGDGGGASAVSGGGEADSDG
                     GRVFDMSEPQEVNNYDQAKGISSGGGGGSTGGVVGRNTGSDGEVGPPPATYERGPTSI
                     GPSVIKTADLRLQLPRDTFDASVRKATTVAERYGGYIISSGIEDRERNSASATLRVPA
                     DRFGAALGALRGLGEVTNERISGEDVTQEFIDLEARLRNYRAQERVLLDLMARSQTIA
                     DTIRVQNELTGIQLEVERIRGRLRYLNDQASFSTIFVHMVEKGAPPPAKVGVLGKAWE
                     RAVEGFFAVIGAVIVSAGVVVPIAILVALAVLVLRWLRPRLIAWGKGAGA"
     gene            complement(2024..3259)
                     /locus_tag="VNP73_09190"
     CDS             complement(2024..3259)
                     /locus_tag="VNP73_09190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL66134.1"
                     /translation="MFHALRHRDFRLLWAGQTISFIGDGLFKVALTWQALELSPTASA
                     LAIVTIARSVPRLAFLLVGGVISDRVPRRTLMLTADVVQTVGVGAIAFLVTTGDIQVW
                     HLAGLSALIGLAQAFYLPSITAITPELVPKEDLVQANALRSGSQMLAYDFLGPAVGGV
                     LVATLGSAPSFGINAVSFAVSAVALLAIRHRPVPEETEGQRNLRREMLEGLRYARSQP
                     WLWISLVVVAGSNLAFSSALSILIPLHVKNDLAGSASELGAYFAAIGLGGGIAVLATT
                     GLRSRQGHLPASFAAWIVSALAITGAGLSPFIVGVVVCGFVIGAGLQYGNVMWESLMQ
                     AAVPARLLGRVTSFDWFMSLALEPVGLAIAAPVAVAIGASPALISAGLFCTTLLVLGY
                     TRKGVRRADPLSDGAAREA"
     gene            complement(3278..3661)
                     /locus_tag="VNP73_09195"
     CDS             complement(3278..3661)
                     /locus_tag="VNP73_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HWL66135.1"
                     /translation="MPYFGGLFSLAMLALWIYCLIDAIQTDEALVRNLNKMIWIILII
                     LVPVVGSIAWLVAGRPENAPFPGAESFPRSTSARRRGITPRAPLGPEDSPEFMSGLDE
                     RTEQLKRWEDDLKRREDELRRREEE"
     gene            complement(3707..3850)
                     /locus_tag="VNP73_09200"
     CDS             complement(3707..3850)
                     /locus_tag="VNP73_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66136.1"
                     /translation="MRRRIASLAVALVAVLGLSAAPAAAEDFTDCEGTIVLFCPGPVP
                     ITP"
     gene            complement(3975..4047)
                     /locus_tag="VNP73_09205"
     tRNA            complement(3975..4047)
                     /locus_tag="VNP73_09205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4012..4014),aa:Ala,seq:ggc)
     gene            4107..4517
                     /locus_tag="VNP73_09210"
     CDS             4107..4517
                     /locus_tag="VNP73_09210"
                     /inference="COORDINATES: protein motif:HMM:NF023075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HWL66137.1"
                     /translation="MTPSEIGERTEAAVLAALSSLGKAVLIPFGPRRYDLAFDEAGKL
                     VRVQCKAGRQVAGAIHFRTHSVGRSALRDYRDDIDLFGVYCHEREEVYLVPVHDVPLR
                     GARLRLEPPKNGQRAGIRAADKYLVMKGMPRLID"
     gene            4571..4909
                     /locus_tag="VNP73_09215"
     CDS             4571..4909
                     /locus_tag="VNP73_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWL66138.1"
                     /translation="MQIEAPVACCAPLAATSVTDDEANATAALFKALADPHRVKILNL
                     LANAGESVCVCEITPRLDLGQPTVSFHLKKLCDSGLLVREQRGTWAYYSIDPEAMNRL
                     RGVVDMEVKA"
     gene            4906..5691
                     /gene="arsM"
                     /locus_tag="VNP73_09220"
     CDS             4906..5691
                     /gene="arsM"
                     /locus_tag="VNP73_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWL66139.1"
                     /translation="MSDVREEVRERYAEAARAASANKTDAAAFESNEAPCCGPDCCGG
                     STAEAVDTPVFGSSLYKELEDGAVPDTALLASLGCGNPTAVAELREGETVLDLGSGGG
                     IDVFLSATRVGPTGKAYGLDMTDEMLELARTNQKKAGVENVEFLKGYIEDIPLPDASI
                     DVVISNCVINLSGDKQAVFNEMARVVKPGGRIGVSDVVASNDLSSEERAQRGSYVGCI
                     AGALSFKEYEDGLTAAGFVDIEVTPTHSVADEMFSAIVKAERP"
     gene            5691..6164
                     /locus_tag="VNP73_09225"
     CDS             5691..6164
                     /locus_tag="VNP73_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014928482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HWL66140.1"
                     /translation="MFRTAASKKQVDVRLPSGQSDDTPLVLFVCVHNAGRSRMAEAIF
                     NSLAAGRYSGRSAGTEPADRPHPEVVEVMSETGYEIDDGPGTLLTPELANSATRVIGM
                     GCAVEEACPALSVPLEDWELDDPKGKSHDEVEMIRDQIEMRVRNLIAQLDRANTG"
     gene            complement(6161..6517)
                     /locus_tag="VNP73_09230"
     CDS             complement(6161..6517)
                     /locus_tag="VNP73_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66141.1"
                     /translation="MRALPRERPATLSAPEDGQKLEELIGDLQSVWGVPQYPQEYRIT
                     VKVQEDRDRSEPAMVVEDQSDSSDQGRGPGDSDVVVREKAPAAPLMRNRGGGDGVKRE
                     KAPRRRRRRRKGGGSG"
     gene            complement(6681..7085)
                     /gene="rsfS"
                     /locus_tag="VNP73_09235"
     CDS             complement(6681..7085)
                     /gene="rsfS"
                     /locus_tag="VNP73_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWL66142.1"
                     /translation="MNERSNGLTSLVLDAAKAASSKKAQDVIVLDVSKLLVITDHFLI
                     CSGTSDRQVRTIADEIEKTLREDHSVKPYRREGEREARWVLLDYVDFVVHVFQTADRE
                     YYDLERLWADAPRFVIEESEGGRDEPAAAKAT"
BASE COUNT         1348 a         2331 c         2239 g         1319 t
ORIGIN      
        1 gatcgagtcg tggatcgaga agatctcgac gtgacccatg tgcatatcgc ccgacggata
       61 gggatacatc gtgagcgcgt agaacttctt ggaccggtcc gggatctcgg gtgccgccca
      121 gtacttgccc tcgatccatt ccttcatcca gcgctgttcg atggcagcga agtcgtactc
      181 gcgttccatg catggaggct agcgcgacag tcgaggccgt cgcgcgtgag gctagcctga
      241 gctacaggtg ccgaaaggcg acgtctggaa ctccttgaag cgatctaacc gatcgatcgg
      301 aacctggtct gtctcggttg agacttgctc gagagtcccg ccatcgatgc tgtaggtggt
      361 tcgcgtcacg tcgtagagcg acttctctag ggcttcgttc ccctgccccg gcacggcccg
      421 ggttacgaac agctcctcgt cgcccgcgca atcgaccgct tcgagatggc ccacgcttcc
      481 gccgtaagcg aacaatccca tctccgactc cggcaactcg atccacgcca ggtcgccgtc
      541 gaccaacgtg tacacgcccg cgaactgggt cgaggctccg cccgcctcgt cgacgacgat
      601 ctcgagtccc ggtttggcat ccacgtcggc gagcgtcttg atcgacggct gcggtagacc
      661 gcccgtcgac cccgtcaacc agatcgggat cgacacgttg ccactcgacg tccccacgat
      721 cagaaaggcc tcgcaaccgg gctcgccgtt ctcgtcgaga acgatctgga cggtgtctat
      781 ctctccgtct ccgtccacat ctccctcgac cgatcccccg ctccctaaag aaacgtcttc
      841 gatcacctgt tgttcgtctg tgcaatcgag gttggcccgg gcgcgtggcg agcttgcggc
      901 cccctccggc tgcgtatcgg gggtgttatc gcaaccggcc atagcgatga ccatcgctgc
      961 ggcgaggatc tgcaccggga accgggaaca tcttgcgtac ctcatctcgt ctgagtgtcg
     1021 taaccgctcg gaccgaggag gtcgaaatga tcggcagtaa gcgtgtgggt gccgcggttt
     1081 tggttctcgt tcttgtcttt gtcctgggtg cttgcagtgg aggcgacggc ggcggcgcat
     1141 ctgctgtgag tggcggcggc gaagcagatt ccgacggcgg ccgggttttc gacatgtccg
     1201 aaccgcagga ggtcaacaac tacgaccagg ccaaaggaat ctcctcggga ggcggcggcg
     1261 gttcgacggg aggcgttgtt ggcaggaaca ccggatcgga cggagaggtt ggccctccac
     1321 cggctactta cgaacgcggc ccgacgagca tcggaccgtc cgttatcaag accgccgatc
     1381 ttcgcctcca attgccgcgc gacacgttcg acgcgtccgt acgaaaggcg accacggtcg
     1441 cggagcgata cggcggctac atcatctcga gcgggatcga agaccgcgag cgcaacagcg
     1501 cgtctgcaac cttgcgtgtc cccgcggaca ggttcggggc cgcgctggga gcgcttcgcg
     1561 gcctcggcga agtgacgaac gagcggatct ccggcgaaga cgtgacgcag gagttcatcg
     1621 acctggaagc tcggttgcgc aactatcggg ctcaagagcg ggtccttctc gatctcatgg
     1681 cgaggtctca gacgatcgcc gacacgataa gggtgcagaa cgaattgacc gggatccaac
     1741 tcgaggtgga gcggatcaga ggtcggttgc gctacctcaa cgaccaggcg agcttttcga
     1801 cgatcttcgt gcacatggtc gagaagggtg caccgcctcc ggccaaggtc ggtgtgctgg
     1861 gcaaggcgtg ggaacgggcg gtggaaggat tcttcgccgt catcggtgca gtcatcgttt
     1921 cggccggcgt cgtcgttccg atcgcgatcc tcgtggctct agcggttctg gtgctgcgct
     1981 ggctgcgtcc ccggttgatc gcgtggggca agggggccgg agcctaggcc tcgcgagcgg
     2041 cgccgtccga aagggggtcg gcccgtctga cccccttccg ggtgtagccg agcaccagca
     2101 atgtggtgca aaaaagaccg gcggagatga gcgcaggcga ggctccgata gccaccgcca
     2161 ccggcgcggc gatggccaga cccacgggtt ccaaggccag cgacatgaac cagtcgaacg
     2221 aagtaacgcg gccgagcaat ctcgccggaa cagccgcttg catcaaggat tcccacatga
     2281 cgtttccgta ctgcagtccc gcgccgatga cgaagccaca caccaccact cctacgatga
     2341 agggcgacaa ccccgccccc gtgatcgcga gagccgacac gatccaggct gcgaacgacg
     2401 ccggtaagtg tccctgacgc gatcgcaggc cggtggtggc gagcacggct atccctccgc
     2461 ctaggccgat cgccgcgaag tacgcgccga gctcggacgc gcttcccgcc aaatcgttct
     2521 tcacatggag cgggatgagt atcgacagcg cactcgagaa cgcgaggttg gacccagcca
     2581 cgactaccaa tgagatccag agccagggct gtgaacgcgc gtaccgcagt ccttccagca
     2641 tctcccggcg caggttcctt tggccttcag tttcctctgg caccggcctg tgccggatcg
     2701 ccagcaacgc caccgcgctg acggcgaacg agaccgcgtt gataccgaac gacggcgcgc
     2761 tgcccaatgt tgcgacgagg actccaccga cggcgggacc caggaagtcg taggcaagca
     2821 tctggctgcc tgatcggagc gcattcgcct gaaccaggtc ctctttggga acgagctccg
     2881 gcgtgatcgc ggtgatcgag gggaggtaga acgcctgcgc caacccgatg agggccgata
     2941 ggccggccag gtgccaaacc tggatgtcgc cggtggtcac gaggaacgcg atggccccga
     3001 ctcccacagt ctgaaccaca tccgcagtga gcatcagcgt gcggcgtggg acgcggtcgc
     3061 tgatcacacc gccgaccaga aggaacgcta gtcgcgggac cgaccttgcg atcgttacga
     3121 tcgcgagggc gcttgcggtg ggagaaagtt cgagggcctg ccacgtgagt gcgaccttga
     3181 acaagccgtc gccgataaaa gagatcgtct gccctgccca cagcagacgg aagtcgcgat
     3241 gccgcagcgc atggaagatc gggttagccg tgggtcgtta ctcctcttcc cggcgacgga
     3301 gttcgtcttc cctgcgcttg aggtcgtcct cccaacgctt cagctgctcg gtcctctcgt
     3361 cgagccccga catgaactcg ggcgagtcct cggggccgag cggcgcgcgt ggagttatcc
     3421 ctctgcgacg ggcggaagtc gacctgggaa agctctccgc cccggggaac ggggcattct
     3481 ctgggcgacc ggccacgagc catgcgatcg agccgacgac cgggacgagg atgatcagaa
     3541 ttatccagat catcttgttg aggttgcgga cgagcgcttc gtcggtctgg atcgcatcga
     3601 tcaggcaata gatccacagg gccagcatgg ccagtgagaa gagtccgcca aagtagggca
     3661 tggctcaagc gtatccgcca tgcctacctc ggcttggaac gaaaagctat ggcgtgatgg
     3721 gaacgggacc gggacagaac agcacgatcg ttccttcgca atcggtgaag tcctcggcgg
     3781 cagcgggggc cgcggacagc ccgaggaccg cgacgagggc aaccgctagc gatgcgatgc
     3841 gtcgtctcat aacacctccc tcgtagctgg ggagcgactt tgatcttgtc ttgtgtcgat
     3901 ccttcgccgc gctcctgcgc tccaccttcc cgaatgagga ctttctctgc ggatcgagcg
     3961 aggggcgaat tgagtggacc cggtcggaat cgaaccgacg acctcctgac tgccagtcag
     4021 acgctctacc aattgagcta cgggcccgaa ggtccgacag tctatgaccc tccgttcttg
     4081 tcacaccccg tcgctacggt cacggaatga ccccatcgga gatcggcgaa cggactgaag
     4141 cagcagtgtt ggcagcactg tcgtctctcg ggaaagccgt gctcatccct tttggacccc
     4201 ggcgttacga ccttgccttc gacgaggcgg gaaagctcgt aagggtgcag tgcaaggcgg
     4261 gccgccaagt agccggagcg atccactttc ggacccatag cgtgggccgg agtgcgctcc
     4321 gcgattaccg agacgacatc gacctcttcg gcgtctattg ccacgagcga gaagaggtct
     4381 acctcgtccc ggtccacgac gtcccccttc gtggggcccg cttaagactc gagccgccaa
     4441 agaacggtca gcgagccggg atccgagctg ccgacaagta cctcgtcatg aaaggcatgc
     4501 cccgactcat agattgacaa tcatcgatat agtgttaaca tcgacatcca tcgatatata
     4561 ggagatgccg atgcagatcg aagcccccgt cgcttgttgc gcccccctgg cggcgacctc
     4621 ggtcaccgac gacgaggcga acgccacggc ggccctcttc aaagccctcg ccgacccaca
     4681 ccgcgtcaag atcctgaacc tgctcgccaa cgccggtgag tccgtgtgcg tgtgcgagat
     4741 cactccccgg ctcgacctcg gccaaccgac cgtcagcttc caccttaaga agctgtgcga
     4801 ctccggtctt ctcgtgcgcg aacagcgggg cacgtgggcc tactactcga tcgatcccga
     4861 agcgatgaac agactccgcg gagtcgtaga tatggaggtg aaagcatgag cgatgtgcgg
     4921 gaagaagtca gagagcgtta tgccgaggca gctcgcgcag caagcgccaa caagaccgac
     4981 gcggccgcgt tcgaatcgaa cgaggcacct tgctgcggcc ccgattgctg cggcggttcc
     5041 actgccgagg ccgtcgacac tccggtgttc ggttcgagcc tctacaagga gctcgaggat
     5101 ggtgctgtgc ccgacactgc cctgctcgcc agcctcgggt gcggcaaccc caccgcggtc
     5161 gccgagctgc gcgagggcga aaccgtcctc gacctcggct ccggcggagg gatcgacgtg
     5221 ttcctgtccg ccacgcgcgt gggacccacc ggcaaggcct atggtctcga catgaccgac
     5281 gagatgctcg agctggcccg cacgaaccag aagaaggccg gtgtcgaaaa cgttgaattc
     5341 ctcaagggct acatcgagga catcccgctc cccgatgcaa gcatcgacgt ggtcatttcg
     5401 aattgcgtca tcaacctgtc gggcgacaag caggccgtct tcaacgagat ggcgcgagtc
     5461 gtcaagcccg ggggccgcat cggtgtctcc gatgtcgtcg catccaacga cctgtcctcc
     5521 gaggagcgcg cgcagcgcgg tagctacgtc ggctgcatcg caggcgcgct ttccttcaag
     5581 gagtacgaag acggcctcac ggccgcaggt ttcgtcgaca tcgaagtcac cccgacccac
     5641 tcggttgcag acgagatgtt ctcggcgatc gtgaaggccg aacgcccctg atgttccgta
     5701 ccgccgcctc gaagaagcag gtcgacgtcc ggctcccatc cggacagagc gatgacactc
     5761 ctctggtcct cttcgtctgc gtccacaacg ccggccggtc gcgtatggcc gaagccatct
     5821 tcaactcact ggcggcgggc cgttacagcg gccgctccgc cggaacagag ccggccgacc
     5881 gaccgcatcc cgaggtcgtc gaagtcatgt ctgagaccgg ttacgagatc gacgacggcc
     5941 ccggcacatt gctaaccccc gaacttgcga actccgcaac cagggtcatc gggatgggat
     6001 gcgccgtcga agaagcctgc ccggctctca gcgtccccct cgaggactgg gagctcgacg
     6061 atccgaaggg caagtcgcac gacgaggtcg agatgatcag ggatcagatc gagatgcgcg
     6121 ttcgtaacct gatcgcgcaa ctggatcgcg cgaacacggg ttaacccgag ccaccacctt
     6181 tccttcttct gcgtcgacgc cgcggggcct tctcgcgctt gacgccatcc cctcccccac
     6241 ggttgcgcat caacggggcg gcgggcgcct tctctctgac gacgacatca ctatcccccg
     6301 gcccgcggcc ctggtctgaa gaatcggatt gatcctccac caccattgcc ggctcggagc
     6361 ggtcgcggtc ttcttgcacc ttcacggtga tccgatattc ctgtgggtat tgcggcacgc
     6421 cccagacgga ctgaaggtcg ccgatgagtt cttccagctt ctggccgtcc tcgggggcag
     6481 aaagtgtcgc gggccgctcg cgcggaagag cccgcaccag atcttccgag gacgtcggcg
     6541 atttcggttc ctggggcttt cctctacgtc cgcgcctccg tcgggttcgc ctctgctggc
     6601 gcggctcttg cgaggcttgc tcgcgcctaa ctgactgctc gcgctgctgt tcgccggtac
     6661 ggggtggtcc gcgtcggcgc ctacgtcgcc ttcgccgcgg ctggctcatc acgcccccct
     6721 tcggactctt cgatcacgaa tcgtggagcg tctgcccaca accgctcgag gtcgtaatac
     6781 tcccggtcgg cggtctggaa tacgtgcacg acgaagtcca cgtaatcaag cagcacccag
     6841 cgcgcttccc gttcgccttc tctccgatac ggcttgaccg aatgatcctc gcgcaacgtc
     6901 ttttcgatct cgtccgcgat cgtccgcacc tgccggtccg aggttccact gcagatcagg
     6961 aagtggtcgg tgatgaccaa tagtttggag acgtcaagaa cgatgacgtc ttgagctttc
     7021 ttagaggagg cggccttcgc ggcatcgagc acgagactcg tgaggccgtt tgatcgttcg
     7081 ttcaaagggt gtcgaggaag tcctttccga caacgattgt caggtcaacg ataccctgct
     7141 gttgccccgc gatctggact tctcccacgc ctagaagctc tttggctcgt tctgctagat
     7201 ccaggccttt ctcggagtcg tcgtacgaaa cgatcaa
//
LOCUS       DATMED010000356         4489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_300965, whole genome shotgun sequence.
ACCESSION   DATMED010000356 DATMED010000000
VERSION     DATMED010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300965"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1890
                     /locus_tag="VNP73_09240"
     CDS             136..1890
                     /locus_tag="VNP73_09240"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWL66143.1"
                     /translation="MCQTCGCWEPDARPKSRSRGEAQWALGYREPQSLAEQTKKEDDG
                     LNVRARIEDVYSKKGFASIWPGDLRSRFRWHGLYTQRPEVDGYFMLRIRIPGGQLTSE
                     QTDVIGRISERYGRDVADVTDRQNVQLHWIRIEDMPEIWRELEAVDLSTTEACGDTPR
                     NLLSCPLAGIDSTEVIDASEVLRNANAALVNDYAFSNLPRKYKISISGCAHQCAQHEI
                     NDVGLVGVKHPSGEVGFDVWVGGGLSTNPMFGKRLGAFVPPDQVVPVVLGITSVFRDW
                     GYRRVRNRARLKFLVQDWGPMKFREVLEEELGFSLEDLDPPPTPNGLVHREHVGIGKQ
                     KDGKSYIGFAPKAGRIQGHQLRAIARLARRFGDGRLRTTTQQKMVILDVEPGRVDELV
                     EELDALDLPVLATNWRRGAMACTGIEFCKLAIVETKGRAVEIYRYLEKALPGYEDEIR
                     INVNGCPNSCARYQTADIGLMGCIISERTWVMDHDGNQIEERRKVEAFLVHLGGHLGT
                     DRSFGRKAKGVKVLASEAGPYIETLIRRYHADRDDAERFSTFVNRLSDKEFLEFSRKP
                     VFHNLPPAPTVIAEPRAS"
     gene            1887..2645
                     /locus_tag="VNP73_09245"
     CDS             1887..2645
                     /locus_tag="VNP73_09245"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWL66144.1"
                     /translation="MTNPDPLPNGFVSPDIHYADIDTDDPRRILGWAAQTIDRLAIAT
                     SFQASGLVMLHMLQSIRPDIPVLFLNTGFHFKETLEFRDEIAARWNLKLVDLRGEHGS
                     AEKQAEIHGPELYRRDPDKCCFINKVEPLQKALQEYDGWISGLRRDQSPMRADTPVVE
                     AQLLQSGNEVLKIHPLAHWTKAQVAGYTLEHGIPTHPLLERGFASIGCWPCTRALKAG
                     ENERDGRWEGLSKTECGIHMFGKDRGPKQSEAEE"
     gene            2650..2790
                     /locus_tag="VNP73_09250"
     CDS             2650..2790
                     /locus_tag="VNP73_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Insertion element protein"
                     /protein_id="HWL66145.1"
                     /translation="MSMRAVPFYCPYCAEEELEPVGEAGGYYCSSCDRRFELKFLGLG
                     QS"
     gene            complement(2891..3946)
                     /locus_tag="VNP73_09255"
     CDS             complement(2891..3946)
                     /locus_tag="VNP73_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWL66146.1"
                     /translation="MAEAEIISLEGRNRGDRAGSPASRIRCRATTSSGRPCRNYAVRN
                     SEYCKTHTTQEQSRSRHPSARSTHPILSDEPDPTDMGVQEWIKRRLAGDYEVDAFGYD
                     PELARGVLLQLAKPLYENYFRVKALGLERIPSEGPALLVGNHSGTIALDAVMMQYAVA
                     TRHPQKRAVRNVAADLVFQLPFVGPFARKTGNAVACDEDADELLRRGELVGVFPEGYK
                     GVGKGWRERYKLQRFGRGGFMEVALRMRVPIIPVAIVGAEEAYPMIANAKLIAKAFGF
                     PYFPITPTFPLLGPLGLIPFPSKWRIEFGDPIPMDDYPEDAAEDAMLVFDLADRVRDT
                     IQQMLYRNLNARRGVFL"
     gene            complement(3946..>4489)
                     /locus_tag="VNP73_09260"
     CDS             complement(3946..>4489)
                     /locus_tag="VNP73_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66147.1"
                     /translation="DLVILRTQNLLGPTIHSNLASYFQLPVIPTALGYDPRLQFLHEV
                     DAVDALYRASMTECKGIFNVAGDGALYLSKAIRLLGKIQVPLLLPVAQLAAEALRSAG
                     IVDFPTDQLKLIVYGRVVDTRRAKEAFGFSPRFSTEEALVDFRDHRAGEQEPGGSAHG
                     SWERELFEYLKQRTAVKESV"
BASE COUNT          861 a         1404 c         1432 g          792 t
ORIGIN      
        1 tcgagggcct gaaccagctg gagcgccacc tcacggagat ctgccgtgac ctggcggcca
       61 gttgacttaa tactgtttac acagtagata taggacttcc gatcgacaga tcaagagtcg
      121 caggaggtcc gtcgcgtgtg ccagacctgc gggtgctggg agcccgacgc ccgtcccaag
      181 tcccgctcca gaggtgaggc ccagtgggcc ctcggttatc gcgagcctca gagcctcgcc
      241 gaacagacca agaaggaaga cgacgggctg aacgtccgcg cccggatcga ggacgtctac
      301 tcgaagaaag gcttcgcctc gatctggccg ggggacctcc gtagtcgctt ccgctggcac
      361 ggcctctata cgcagaggcc ggaggtcgac ggctacttca tgctgcggat acgcatcccc
      421 ggagggcaac tcacgtccga gcagaccgac gtgatcgggc ggatctccga gcgctacggg
      481 cgggacgtcg ccgacgtcac cgatcgtcag aacgtccagc tccactggat ccggatcgaa
      541 gacatgcccg agatctggcg cgagctggaa gccgtcgacc tgtccaccac cgaagcctgc
      601 ggggacacgc ctcgcaacct gttgtcgtgc ccactagcgg ggatcgactc gacggaagtg
      661 atcgatgcat cggaggttct gcgcaacgcg aacgccgcgc tcgtgaacga ctacgcgttc
      721 tcgaatctgc cgcgcaaata caagatctcg atctcgggct gcgcccatca gtgcgcgcaa
      781 catgagatca acgacgtcgg gctggtgggt gtaaagcacc cctccgggga agtgggcttc
      841 gacgtgtggg tcggcggcgg gctgtcgacg aatccgatgt tcgggaaacg cttgggtgcg
      901 ttcgtgccgc ccgatcaagt cgtcccggtt gttttgggga tcacgagtgt gttccgagat
      961 tggggatatc gcagggtgcg caaccgggcc cggctcaagt ttctcgtgca ggactggggg
     1021 ccgatgaagt tcagggaggt gctcgaggag gaactcggtt tctcgctcga ggacctggac
     1081 ccgcctccca cgccgaatgg cctcgtgcat cgcgagcacg tcgggatcgg caagcagaag
     1141 gacggcaaga gctacatcgg gttcgcgccg aaagcgggcc ggatccaggg ccatcagctg
     1201 cgggctatcg cgcgtctcgc tcgtcgtttc ggtgacgggc ggctccgcac gaccacgcaa
     1261 caaaagatgg tgatcctcga cgttgagccc ggccgcgtag acgaactcgt cgaagagctc
     1321 gacgcactcg acctccctgt tcttgcgacc aactggcgtc gtggcgcgat ggcatgcacg
     1381 gggatcgagt tctgcaagct ggcgatcgtc gagacgaaag gccgcgccgt cgagatctat
     1441 cgctacctgg agaaagcact tcccggctac gaagacgaga tacggatcaa cgtcaatggg
     1501 tgccctaact cgtgtgcgcg ctatcagacg gcggacatcg gcttgatggg ctgcatcatc
     1561 agtgagcgca cctgggtgat ggaccacgac ggcaaccaga tcgaagagcg ccgcaaggtc
     1621 gaggcgttcc tcgttcacct cggcgggcac ctcggaaccg atcgttcctt cggccgcaag
     1681 gccaagggcg taaaggtgct cgcgtccgaa gcgggtcctt acatcgagac cctgatccgc
     1741 aggtaccacg cggatcgcga cgacgctgag cgcttctcga cattcgtgaa ccgcctgtcg
     1801 gacaaggagt tcctggagtt ctcccgcaag cccgtgttcc acaacctgcc gccggccccc
     1861 accgtgatcg cagaaccgag ggcttcgtga ctaacccaga cccgctgccc aacggattcg
     1921 tctctccgga tatccattac gcggatatcg acaccgacga tccgcggagg atcctggggt
     1981 gggccgcgca gacgatcgat cggctcgcga tcgcgacctc gttccaagcg tcgggactcg
     2041 tcatgttgca catgctgcag agcatcaggc ccgatatccc cgtgttgttc ttgaataccg
     2101 gtttccactt caaagagacg ctcgagttcc gcgacgagat cgcggcacga tggaacctga
     2161 agctggtcga cctgcgcggt gagcacggct cggcggagaa acaggccgag atccatggac
     2221 ccgagctcta tcggcgagat cccgacaagt gctgcttcat caacaaggtc gaacctctgc
     2281 agaaggcgct tcaggaatac gacggctgga tctcgggtct gcgtcgagat caatcaccaa
     2341 tgcgcgccga cacccccgtc gtcgaagctc agctgctgca gtcgggcaac gaggtgctga
     2401 agatccaccc gctcgcgcat tggacgaagg ctcaggtcgc cggatacacg ctggaacatg
     2461 ggatcccgac ccatccgctc ctcgagagag gtttcgcctc gatcggttgc tggccgtgca
     2521 cccgggcgct gaaggccggc gagaacgaaa gggacggtcg ctgggagggt ctgagcaaga
     2581 cggagtgtgg gatccacatg ttcggcaagg atcggggacc caagcagagc gaagctgagg
     2641 agtagctcga tgagcatgcg tgcggtcccg ttctactgcc cctattgcgc cgaagaggaa
     2701 ctagagccgg tcggcgaggc gggcggctac tactgctcgt cgtgcgatcg ccgcttcgag
     2761 ctgaagttcc taggtctcgg tcagtcctag aagccgatct gccacatccg ccacgcgccc
     2821 aatccgccgg cgatcgttat agcggcaaga agaaacgcca gcgcgagcag ggtgttacga
     2881 cgcattgcct ctagagaaag acgcctcggc gggcgttgag gttgcggtac agcatctgct
     2941 ggatcgtgtc tctcaccctg tcggccaggt cgaacaccag catggcgtcc tcggccgcat
     3001 cctccggata gtcgtccatc gggatgggat cgccgaactc gatgcgccac tttgacggga
     3061 acgggatgag cccaaggggg ccgagcaggg ggaacgtggg agttatcggg aagtacggga
     3121 acccgaacgc cttcgcgatc aacttcgcgt tcgcgatcat cggataggcc tcttcggccc
     3181 ccacgatcgc gaccgggatg atcggaacgc gcatccggag agcgacctcc atgaagccgc
     3241 cacggccgaa ccgctgcagc ttgtagcgct cacgccagcc cttgccgacg cccttgtagc
     3301 cctcggggaa gactccgacg agctcgcctc gacgcagcag ttcgtctgca tcctcgtcgc
     3361 aagcgaccgc attgccagtc ttgcgagcga acggccccac gaacgggagc tggaacacga
     3421 ggtcggcggc aacgttgcgg accgcacgtt tctgcgggtg ccgggtcgcg actgcgtact
     3481 gcatcatcac cgcgtcgaga gcgatcgtgc ccgagtgatt cccgaccaat aaggccgggc
     3541 cctcgctggg gatccgctcg agccccagag ccttcacccg gaaatagttc tcgtagagcg
     3601 gcttcgccag ttgcagtagg acgccgcgcg ccagctcggg gtcgtagccg aaggcgtcga
     3661 cctcgtaatc cccggccaac ctgcgcttga tccactcctg cacgcccatg tccgtcgggt
     3721 ccggctcgtc actaaggatc ggatgagtcg agcgcgccga cgggtgtctg ctgcgcgact
     3781 gctcctgcgt cgtgtgggtc ttgcagtact cgctgttgcg caccgcgtag ttacggcacg
     3841 ggcgtccact cgacgttgtc gcgcggcacc tgatccggga cgccggcgag cccgcccggt
     3901 cgccacggtt cctaccttcc agcgagatga tctcggcttc tgccatcaca cgctctcttt
     3961 cactgcggtt cgctgtttga ggtactcgaa cagctcacgc tcccaacttc cgtgagcgga
     4021 gccgccgggc tcctgttcgc cggcacgatg gtctctgaag tcgaccagcg cttcctccgt
     4081 cgagaatcga ggcgagaacc cgaaggcctc tttggcccga cgcgtatcta ctacccgtcc
     4141 gtagacgatc agcttcaatt ggtcggtggg gaaatcgacg atccccgcgc ttcgtaaggc
     4201 ttcggcggcc agctgagcca ccggcagaag caagggaacc tgaatcttgc cgagcaaacg
     4261 gatcgccttg gacaggtaca acgctccgtc gcctgcgacg ttgaagatcc ccttgcactc
     4321 ggtcatcgag gcgcgataga gagcgtcgac cgcgtcgacc tcgtgcagaa actggaggcg
     4381 agggtcgtaa cccaatgccg tcggtatcac cggaagctgg aagtaggagg cgagattgct
     4441 gtggatcgtg gggccgagca ggttctgcgt cctgaggatc acgagatcg
//
LOCUS       DATMED010000357         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_301172, whole genome shotgun sequence.
ACCESSION   DATMED010000357 DATMED010000000
VERSION     DATMED010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301172"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VNP73_09265"
     CDS             complement(<1..394)
                     /locus_tag="VNP73_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWL66148.1"
                     /translation="MSERAKKKVAVIFGGRSVEHDVSIVTAHQVMAALENRHEVVPVY
                     VTKEGELWTGPALNDLAVFKDTRWSEAGVRCSFTQGPDGGLALPPKRKGLGVETVVER
                     ADVVVCSIHGTFGEDGTLQGLLELAGIPY"
     gene            complement(417..1934)
                     /gene="murF"
                     /locus_tag="VNP73_09270"
     CDS             complement(417..1934)
                     /gene="murF"
                     /locus_tag="VNP73_09270"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWL66149.1"
                     /translation="MRPDPFVGYSLLLRVIIYGAVPAFTAWQFVRLRRALHVFQLEGY
                     KRARFLDWCKQNRDRARFLSRSTLKKPLVMTGRAWRILLVATLLTVALLLGPAAGGHL
                     AWGAPADLIAWALSTVVAFWFAPQILVAADVLLAPVQKAINSSYERGARAKLARIGPE
                     VVGVTGSFGKTSTKVAIAGLLGGTAYPTPGSYNTPMGVCRAINEGLDGSHRFFIVEMG
                     AYQRGEIAELCRLVEPKVGVLTAIGPAHLERFGSLDTIRAAKYEIVQALAPSGVAVMN
                     VDDPEVRALADKTDHVRVIRYGIDVAGRPDVTATEIRVSERGTQFIVGSAAGSAPAGM
                     RLLGRHAIGHVLAAVGVGIALDRSLEELVKRIGELEPAEHRLQVIPGAQGVTVIDDAY
                     NSNPDGAASALEVLDAMPGERKVVVTPGIIELGSKQAEANERFGEAAAEIADTVIVVA
                     RVNRDALVKGAERAGGAAEVITVDSLDQATEELKRLLRAGDVVLFENDLPDQYEN"
BASE COUNT          388 a          715 c          616 g          362 t
ORIGIN      
        1 catacgggat cccggcgagc tcgagcagcc cctgcagcgt tccgtcctcg ccgaaggtcc
       61 cgtggatcga gcaaacgacc acgtcggccc gctcgaccac ggtctcgacc cccaagcctt
      121 tgcgtttcgg aggtagggcc agccccccgt cgggtccctg cgtgaacgag caccgaacgc
      181 cggcctcgct ccaacgggtg tccttgaaca cagcaagatc gttcagagcg gggcccgtcc
      241 agagctcacc ttctttggtg acgtagaccg gcacgacctc gtggcggttc tccagggcag
      301 ccatcacttg atgggcggtc acgatggaga cgtcgtgctc caccgagcga ccgccgaaga
      361 tgacggcaac cttctttttc gcgcgttcgg acatagtccg agcagtgtaa gtgggttcag
      421 ttctcgtact ggtcgggcag gtcgttctcg aagagcacca cgtcaccggc acggagcagt
      481 ctcttgagct cctccgttgc ctggtcgagg gagtcgacgg tgatgacttc ggcggccccg
      541 ccggcgcgct ctgcgccctt tacgagggca tcacgattca cacgcgccac gacgatcacg
      601 gtatcggcga tctctgcagc ggcttcaccg aacctctcgt tcgcctcggc ttgttttgag
      661 ccgagctcga tgatccccgg agtgaccacg accttcctct cgcccggcat cgcgtcgaga
      721 acttccaacg cagatgccgc accgtcgggg ttcgagttgt aggcgtcgtc gatcaccgtg
      781 acaccttgag ctcccgggat cacctgcagg cgatgctcgg ccggctcaag ttctccgatg
      841 cgcttaacga gttcctccag cgatcggtcg agagcgattc caacgcccac cgcggccagc
      901 acgtggccga tagcgtgccg gccgagcaaa cgcatgccgg caggagcaga tccggcggcc
      961 gatccaacga tgaactgcgt acctctctcg gagaccctga tctcggttgc ggtcacgtcg
     1021 gggcgaccag cgacgtcgat cccatatctg atgactcgaa catgatccgt cttgtcggcc
     1081 agggccctca cttccgggtc gtccacgttc atgacggcga cgcccgacgg ggccagcgcc
     1141 tgcacgatct cgtacttcgc tgcgcggatc gtgtccagag agccgaacct ctcaagatgc
     1201 gcgggcccga tcgcggtgag cactccgacc ttcggctcga ccaggcggca cagttccgcg
     1261 atctcgccgc gctgatacgc gcccatctcg acgatgaaga aacggtgcga gccgtcgagc
     1321 ccttcgttga tcgcgcgaca aacacccatc ggggtgttgt aagagccggg cgtcggatac
     1381 gcggttccgc ccaacagccc ggcgatcgca accttcgtcg atgtctttcc gaaggatccg
     1441 gtgaccccca ccacttccgg tccgatccgc gcgagcttcg ctcgagcacc tctctcatag
     1501 gaagagttga tcgccttctg gaccggagct agtaagacgt cggccgcaac gaggatctgc
     1561 ggggcgaacc agaacgcgac gaccgtcgat aaggcccatg cgatcagatc cgccggcgcc
     1621 ccccacgcga gatgaccgcc ggcagcgggc ccgagcagca gcgccacagt cagcagggtt
     1681 gccacaagca ggatccgcca cgctctaccg gtcatcacga gcggcttctt cagcgtggac
     1741 cggctcaaga agcgcgcccg gtctctattc tgcttacacc agtcgaggaa cctcgctctc
     1801 ttgtacccct cgagttgaaa gacgtgcaga gcgcgcctca atcggacgaa ctgccacgcg
     1861 gtgaacgccg gcaccgctcc gtagatgatc actcgcaaca acaacgagta accgacgaag
     1921 ggatccggcc tcatcgatcc gcggacaggc cgaggaagtg ccgcactacc ttgcagaacc
     1981 gagccgattg atcgaggtat gcgaagtgcc ccccgccctc gaagacgacc aagcctgcgt
     2041 cggggatcag cccttccatc ttctccgcag cagccagagg c
//
LOCUS       DATMED010000358        11442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2229968, whole genome shotgun sequence.
ACCESSION   DATMED010000358 DATMED010000000
VERSION     DATMED010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229968"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..679
                     /locus_tag="VNP73_09275"
     CDS             65..679
                     /locus_tag="VNP73_09275"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWL66150.1"
                     /translation="MPYKLPDLPYDHSALEPHIDAKTMEIHHSKHHQTYVDKLNAAIE
                     GSGLDDKYPDVDDLLRNISEVSDENTKKAVRNHAGGHSNHSIFWSIMSSDGGGEPSGD
                     IADALNTSFGSFKEFQEKFNATATNHFGSGWAWLVSSGGKLDVVGRPNQDSPLMDGDF
                     PILGIDVWEHAYYLKYQNKRPDYIEAWWNVINWDEVNNRFKSAS"
     gene            complement(749..2068)
                     /locus_tag="VNP73_09280"
     CDS             complement(749..2068)
                     /locus_tag="VNP73_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66151.1"
                     /translation="MERRSLVRLGAVVAAVVALWGLPAAPAVAGVEDGSVVIETNTDF
                     TGENGVRKGNGTKKSPYVIEGWDLNTLEIHDTNRYVVIRNNIINRLTLNWVGSKRVKV
                     VQNRVGDLRVNENVERTGAATSGIISHNRFQSVGQLRHWDGVFSHNVVGFVPEDGSFQ
                     IQFGEASTRAVNFDGFNGAKFVHNKIYGYVEARLHGHHHSSGYEQGSHYHGAGHEAAH
                     GGRMPLTHMERFHRVKIANNEIHAVGPYGLIYTDSNHAGNDRTAASETNEALNDPHEH
                     TTQVAIVDNNLHGSGIVVDIFNADDDRHLRTNTGHFTIRENTIRVKEYRNAANAWDAP
                     AAGIDVHQARDLHLHILDNVITGPKGDQTPSAGDTIGGYVTSGIKLQDIEKAYIHLTT
                     NEISSRQVAVYARQFSRVQWWISGMRTQDVVTEVDYDNSSNQPRNGP"
     gene            complement(2220..2915)
                     /locus_tag="VNP73_09285"
     CDS             complement(2220..2915)
                     /locus_tag="VNP73_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66152.1"
                     /translation="MMRIRNLVAVVGSVALLAAALTPGIAQAAKSKPVKTTLYLHGTT
                     PIGESESFPAVADMFLPMDATEPTGAEPKSKAITNGVGTPNYQCAGNTLFPVWTGQLV
                     GRVKGDMTLTFHTVGSPGQVEVRVWPDVMSQMCTSEATGATDYVEPAGAVVVDLPPGQ
                     GSVEAVIKGVDFEAVAGLMVQISPIVVADLPSPAGAVLGPNFARVLYDTPDFASALEF
                     TCIPAKGQKSCTP"
     gene            complement(2932..3405)
                     /locus_tag="VNP73_09290"
     CDS             complement(2932..3405)
                     /locus_tag="VNP73_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66153.1"
                     /translation="MKRLVTLILLVVVAAAFVAAPAEAGKKKKVKRVTRTAEAPYAAP
                     GFGTATAAAGCSPALGSCGNFAIGPDDKFVKVTLTDATGTPTAFSVAQDTEPGDPTNT
                     IETNHGTFCGTTGDTPIALVPGAEITIFIWAWGDAVCPTGVGTSGTVTAEFSNLP"
     gene            complement(3547..4032)
                     /locus_tag="VNP73_09295"
     CDS             complement(3547..4032)
                     /locus_tag="VNP73_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66154.1"
                     /translation="MKRLIVLTLVAAIAAAFVAVPADAKKKMKKAPKPRVVEVPYQGG
                     GIGIASPVYTIGACPMTSPGSLECIEIPPMPGEKYVKVEVKDAVPLTPAGFISQGDVD
                     GDGIADGYGEFCGAHAEPVFLEGGMTPVRVSFYPGVCSNAGGASFPTTGTIVATFSTT
                     P"
     gene            complement(4157..4651)
                     /locus_tag="VNP73_09300"
     CDS             complement(4157..4651)
                     /locus_tag="VNP73_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66155.1"
                     /translation="MKKTLAVALMIGLIAASFAVPAEAGKKKKKKAPAPAPVARVERV
                     VELTYEHPSLAVAPVGGFPTNFPEGGTEIPLGPDDLFIKVEVTDASGQKVSGFIGQGD
                     IDGNGVNDDGYGQFCGAHPAPIQVAAPGQTIIGIYLANGVCEDATPSIMTTGSIKVTL
                     SNMP"
     gene            complement(4738..5223)
                     /locus_tag="VNP73_09305"
     CDS             complement(4738..5223)
                     /locus_tag="VNP73_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HWL66156.1"
                     /translation="METKDPLDESPVLVSACLAGVPSRYDATGSFQNEVAKLVAEGRA
                     VPVCPEVDGGLGTPRPPAEIVGGDGADVIEGRARVLTKEGEDVTSAYLKGAHIALEAA
                     QRSGARVAILKARSPSCGRGAIYDGTHSRSLTTGDGVTVALLRKHGIEVLSEEDVAGS
                     S"
     gene            5271..5474
                     /locus_tag="VNP73_09310"
     CDS             5271..5474
                     /locus_tag="VNP73_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWL66157.1"
                     /translation="MSDPNLAGRPLPEDLRGVLEADPVAMGQWNALPQSQKDAWLQYL
                     TEGREPARRAIRIANLLMSITDE"
     gene            5877..6824
                     /locus_tag="VNP73_09315"
     CDS             5877..6824
                     /locus_tag="VNP73_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWL66158.1"
                     /translation="MSRPYESGREEAAHEDLVRQYLREIGQYPLLTDVQEVELARAIE
                     EGEKANEKLQKATKKMTDKQRFSLQSTVRTGRAAKRRFIQSNLRLVVSIAKKYSAAGL
                     PLLDLIQEGNLGLMRAVEKFDYQRGFKFSTYATWWIRQAITRAIADKARTIRIPVHMV
                     ETLYRVRKVQSELLEKLGREPTIEEIAEGSDLSPEKVREAFRVLPEPVSLHEPVGDED
                     AELGDFIEDEDVEGPFQAAAVALRQEDLWDMLGSLSDRERKVLALRFGLVTGEPRTLE
                     EVGKEFNLTRERIRQIEAKALSKLRHPSTPGKLRDMVTI"
     gene            6926..7219
                     /locus_tag="VNP73_09320"
     CDS             6926..7219
                     /locus_tag="VNP73_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWL66159.1"
                     /translation="MITTVVLATCDIHQIPETAQQIADIEEVSEVYSVAGDFDLVIMV
                     RVKTHDDLARVVSEQIARIAGIERTQTLVAFKVFSRHDVEALFSIGFEEEPAS"
     gene            7231..8400
                     /gene="thiI"
                     /locus_tag="VNP73_09325"
     CDS             7231..8400
                     /gene="thiI"
                     /locus_tag="VNP73_09325"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HWL66160.1"
                     /translation="MDALIAHYHEIGLKGRNRDFFERALTRNLKRALRGTGYSRVRHI
                     AGRVLVDLDDETLAQEAALRASRVFGLAYVGAGVRVEPNIDEIGRVALELMQAEPFES
                     FRVRARRSYSSMTETSSEINNVVGQQIKDATEGRVDLKHADATAWIEMFGKTGIVYRN
                     RLEGPGGLPAGTSGRMLALLSGGIDSPVAAWRMAHRGAEVEMVHFHGQPFTDPSSVRQ
                     SLELAEVLTRYQLQTIVHLVPLSDIQREIVTHAPANLRVVLYRRTMMRIAAELALQRD
                     AVALITGDSLGQVASQTIENITTVDSAISGVQVLRPLIGMDKQEIVNEARKIGTYEIS
                     TRDYQDCCVLFQPRSPATRANPGLAERAETELNVDALVGKALAGIETRILELPPP"
     gene            complement(8397..9986)
                     /locus_tag="VNP73_09330"
     CDS             complement(8397..9986)
                     /locus_tag="VNP73_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWL66161.1"
                     /translation="MATTRTRKAKTSSNGQSLKSFNPRTGEVMREIPATNPGEVADIV
                     AQARKVQPEWAAIAPQGRANILKEVRYRIYDLMDDIVETVAAEAGKPRAEALAHDIVP
                     PMLMLSHLERTAPKALRSQKVGGIMGPLLFGTSSRIDWRPYGVVGAITPWNYPITNCF
                     LGFAPALFAGNVVVIKPSEVTPACGELMRQILEPLPPGVANVMQGGGDVGAALVDAPC
                     DKISFIGSPATGRKICEAAAKHLTPVVMELGGKDAAIVLEDADIDVASSGILWGAFFN
                     AGQTCCSIERAFVVDSVADDFEDKLVSKLQLLRQGGEDGDVGSLTFKGQFDKIVGQVA
                     DAKKKGANILAGGEEGRTNEGGSLWFAPTVVKGASEEMDIVKEENFGPILTITRVQDQ
                     EEAIRRANESFNLTASVWTKDRKRADDVATKVKAGGIGSNDHGTLPGMPWAPWGGVGE
                     SGFGRLSGELGIREFTVPTHIAHSLMPGRKKPFWYPYDETTTGLFSSMARLFGSRDVG
                     EKIGAVVSVLGNFSKSAKTRL"
     gene            complement(10094..10891)
                     /locus_tag="VNP73_09335"
     CDS             complement(10094..10891)
                     /locus_tag="VNP73_09335"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWL66162.1"
                     /translation="MEEHANDSDIAIVECDEDITAYEKGHIPNAISIDWANELHDSPR
                     REFVSREQLAELFGKKGISSDQTIVLYSGNNNWFAAYAYWLFKYRGVDDVRLLNGGRK
                     KWELESRLLTQDVPSPSASNFTLGDEKGELRVFREEAIERAKKGNEAWVDVRSPEEFR
                     GELLAPPHLPQEQAQVPGHIPGASNITWSKTVNEDGTFKTADELEKLYAAEGITSDKE
                     VVAYCRIGERSSHSWFVLRELLGFQNVRNYDGSWTEYGSLVGVPIEK"
     gene            complement(10943..11380)
                     /locus_tag="VNP73_09340"
     CDS             complement(10943..11380)
                     /locus_tag="VNP73_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWL66163.1"
                     /translation="MPDLGDDSATRTAKVLSLPTRAAILALLLRSGAKTVKEIADHFQ
                     IHPNVARAHLDLMVEAGFLATETRRRSKGRPAKVYFTWEGEAGNLSEAGVDVPTDRVA
                     QTVDEVGVEVRVLERMLADATHQLARVKELIEETKKLKAEKGR"
BASE COUNT         2078 a         3625 c         3479 g         2260 t
ORIGIN      
        1 tggcacgccg acgccgcggg taggctcaaa atagcggcaa aaagaccaat atcgaggagg
       61 aagcatgccc tacaagctgc ccgatctccc ctacgaccac tcggcgctcg agccccacat
      121 agacgccaag acgatggaga tccaccactc caagcaccac cagacctacg tcgacaagct
      181 gaatgcagcg atcgaggggt cgggcctcga cgacaaatac cccgacgtcg acgatcttct
      241 acgcaacatc tccgaggtgt ccgacgagaa cacgaagaag gcagttcgca accacgctgg
      301 gggccactcg aaccactcga tcttctggtc gatcatgagc agcgatggtg gcggcgagcc
      361 ctcgggcgac atcgccgatg ccctgaacac ttcgttcggc tcgttcaagg aattccagga
      421 gaagttcaac gcgaccgcca cgaaccactt cggttccggg tgggcgtggc tggtttcgag
      481 cggcggcaag ctcgacgtcg tcggccggcc gaaccaggac tcacccttga tggatggcga
      541 cttcccgatc ctcggcatcg acgtgtggga gcacgcctac tacctcaaat accagaacaa
      601 gaggccggac tacatcgaag catggtggaa cgtcatcaac tgggacgagg tcaacaaccg
      661 cttcaagtcc gccagctagc tcgacgtaca aacgcagagg ccgggcgggg gccgcccggc
      721 ctctatcgcg ttcaggggac cctggtgcct agggtccgtt cctcggctga ttagaactgt
      781 tgtcgtagtc cacctcggtt acgacgtcct gcgtcctcat cccgctgatc caccactgca
      841 ccctgctgaa ctgacgtgcg tagaccgcga cctgtcggct gctgatctcg ttcgtggtca
      901 agtggatgta cgccttctcg atgtcctgca acttgatccc actcgttacg tagccaccga
      961 tcgtgtcgcc cgcgctcggc gtttggtcac ctttgggacc ggtaatcacg ttgtcgagga
     1021 tgtgcaggtg caggtcgcga gcttgatgca cgtcgatccc tgcagccggt gcatcccagg
     1081 cgttggctgc gttcctgtac tccttcaccc ggatcgtgtt ttcacggatc gtgaagtgac
     1141 ccgtattcgt ccggaggtgc ctgtcgtcgt cggcgttgaa gatgtccacg acgatccccg
     1201 acccgtgcag gttgttatcg acgatcgcaa cctgagtggt gtgctcgtgc ggatcgttga
     1261 gcgcctcgtt cgtctccgac gccgcggtgc ggtcgttgcc cgcgtggttc gagtcggtgt
     1321 agatgaggcc gtaagggccg accgcgtgga tctcgttgtt ggcgatcttc acccggtgga
     1381 accgctccat gtgggtgaga ggcatccggc ccccgtgggc ggcctcatga ccggctccgt
     1441 ggtaatggct cccctgctcg tagccggatg agtggtgatg accgtggagc ctcgcctcga
     1501 cgtagccgta gatcttgttg tggacgaact tcgctccgtt gaagccgtcg aagttgacgg
     1561 ccctggtgct ggcttcgccg aactggatct ggaacgagcc gtcctcgggc acgaagccca
     1621 cgacgttgtg cgagaagaca ccgtcccagt gacgtaactg gccgaccgac tggaagcggt
     1681 tgtgggagat gatcccgctc gtcgcggccc ccgtccgttc cacgttctcg ttcactcgta
     1741 ggtcaccgac gcggttctga acaaccttca cgcgcttgct ccccacccag ttcagagtga
     1801 ggcgattgat gatgttgttg cggataacga catagcggtt cgtgtcgtgg atctcgagcg
     1861 tgttgagatc ccagccctcg atcacgtacg ggctcttctt ggtcccgttg cccttccgaa
     1921 ccccgttctc gccggtgaaa tcggtgttcg tctcgatgac gacgctgccg tcctcgaccc
     1981 ccgccaccgc cggcgctgca ggcagtcccc acagtgcgac gaccgccgct acgaccgccc
     2041 ccaaccgaac gagagagcgt ctttccatcc cgaccatccc ccttcaaacg acgacaccga
     2101 cccggccttt tccgggcctc gtcaggaaag tacggatatg tccgattctg gtaacggtcc
     2161 aagcaaaaaa aagaggaccc ccgtggaggg gccctctgag atctatctac cgggtgtcgc
     2221 tatggggtac agctcttctg gcctttggcc gggatgcagg tgaattccag ggccgaggcg
     2281 aagtcgggcg tgtcgtaaag gactcgggcg aagttcgggc ccaagactgc acccgccggt
     2341 gacgggagat cggcgaccac gatcggactg atctgaacca tgagtccggc aacagcttcg
     2401 aaatcgacac ccttgatgac ggcttctacg ctgccctgcc cgggtggcag atcgacgacc
     2461 accgctccag caggctccac gtaatcggtt gccccggtag cttccgaagt gcacatctga
     2521 gacatgacgt ccggccaaac gcggacctcg acctgtccag gtgaaccgac ggtgtggaaa
     2581 gtcagcgtca tgtcgccctt cacgcgaccc acaagctgtc cagtccacac ggggaacaga
     2641 gtgttgccgg cacattggta gttgggcgtg cccacaccgt tggttatcgc cttcgacttg
     2701 ggctcggccc ccgtgggttc cgtcgcatcc atcggaagga acatgtcggc aaccgcgggg
     2761 aaactctcgg actcaccgat cggcgtcgtg ccgtgcagat acagcgtcgt cttgaccggc
     2821 ttcgacttcg cagcctgggc tatgcccggg gtcaatgcgg cagcgagtaa cgccaccgaa
     2881 ccgacgaccg cgaccagatt tcggatcctc atcatttagc tcctcgctgc cttacggcag
     2941 gttcgagaac tcggccgtga cggttccgga cgtaccgacg ccggtcgggc agaccgcgtc
     3001 gccccacgcc cagataaaga tcgtgatctc ggctccaggg acgagagcga tcggcgtgtc
     3061 gccggtcgtt ccacagaatg tgccgtggtt cgtctcgatc gtgttcgtcg gatcgcccgg
     3121 ctcggtgtcc tgcgcaacac tgaacgcggt cggcgtgccg gttgcgtccg taagcgtcac
     3181 cttgacgaac ttgtcgtccg gtccgatcgc aaagttgccg cagctaccca gtgcaggcga
     3241 gcaaccggca gcggccgtgg ccgtaccgaa cccgggtgcc gcgtacggag cttccgcggt
     3301 acgggtgact cgcttgacct ttttcttctt gcccgcttcc gcgggggccg ctacgaacgc
     3361 agcagctact accaccagca ggatcagagt tacgagtctc ttcatcttgt tcctcctaga
     3421 tctcagtctt gaaggatgga ttcgcccctc gtgcttccgt tcctgcgatt gacgccgggg
     3481 actaatagag caacggggag caaaacggac aaagctgccc ggccttcaat ttgagttatc
     3541 ggttacttac ggtgtagtcg agaacgttgc cacgatcgtt ccggtggtgg ggaacgatgc
     3601 gcctccggca ttcgagcaca cgccggggta gaagctcacg cgcaccgggg tcatcccacc
     3661 ctcgaggaaa accggttccg cgtgggcgcc acagaactcg ccgtaaccat cggcgatgcc
     3721 gtcaccgtcg acgtcacctt gcgagatgaa acccgccggc gtgagaggca cagcgtcttt
     3781 gacttcgacc ttcacgtact tctcgcccgg cataggtggg atctcgatgc actcgaggct
     3841 ccccggagaa gtcatcggac aggcaccgat cgtgtaaacg ggagacgcga taccgatccc
     3901 accgccttgg tagggaactt cgaccacgcg cggtttggga gccttcttca tcttcttttt
     3961 cgcgtcggcc ggcacggcta cgaaagctgc tgcgatggcc gccaccagcg ttagaacgat
     4021 gagtctcttc atgacgatcc tcctcgaagg gtcttggctt ctgagattcg cctcggctcc
     4081 gctcggttcc tgcgataagg gagaaaagaa aagaggccgg gcatgtttct gcccggcctc
     4141 ttcagagaga tgacttttac ggcatgttgg acaaagtgac cttgatcgag cccgtcgtca
     4201 tgatcgacgg agttgcgtcc tcgcacactc cattcgcgag atagatccct atgatcgtct
     4261 ggccgggggc cgctacctgg atcggtgcag ggtgggcgcc gcagaactga ccgtaaccat
     4321 cgtcgttgac accatttccg tcgatgtcgc cctgaccgat gaaccccgaa actttctgac
     4381 ccgatgcgtc ggtgacctcc accttgatga agaggtcgtc cggaccgagc gggatctcag
     4441 ttccgccctc gggaaagttc gtggggaacc cgccgactgg agcaacggca agactcgggt
     4501 gttcgtacgt aagctcgaca actcgctcga cccgcgcgac tggggcggga gcaggcgcct
     4561 tcttcttctt tttctttccg gcttcagccg gtaccgcgaa cgatgccgcg atcaacccga
     4621 tcatgagtgc gacggcgagt gtcttcttca tcgaggtctc cttactttct agcggggcgc
     4681 ttcaaacaaa atattcgcct ctcgctgtgc cggacctgca gacacaaaag ggattgatca
     4741 ggacgaaccg gcgacgtctt cctcggaaag gacctcgatc ccatgcttac gcagtaaggc
     4801 aacggtgacc ccgtctccag tagtgagcga tcgcgagtgc gtcccgtcat atatcgctcc
     4861 gcggccgcac gaggggctac gagctttgag gatcgcgacc cgggcgccgc tccgttgtgc
     4921 cgcttcgagt gcgatgtggg cccccttcag gtatgcggag gtgacgtctt ctccctcctt
     4981 ggtcagcacc cgagctcggc cctcgatcac atcggctccg tcccctccga ctatctcggc
     5041 tgggggccgg ggggttccga gtcccccatc gacctccggg cacacgggca ccgcacgccc
     5101 ctccgccacg agtttcgcca cctcgttctg gaagctgccg gtcgcgtcgt agcggctcgg
     5161 caccccggcg aggcaggccg agaccagaac cggggactcg tctagaggat ctttcgtctc
     5221 cacagaaaaa gtcctatcgc aggggggccg cgctggtgtt ggattggatc atgagcgatc
     5281 cgaacctggc gggtcggccg ctccctgaag acctgcgcgg ggtactggaa gcagaccccg
     5341 tcgcgatggg gcagtggaac gcgctaccgc agagtcagaa ggatgcctgg ctccaatacc
     5401 tgaccgaggg ccgtgagcct gcccgccggg cgatccggat agccaacctg ctgatgtcca
     5461 ttaccgacga ataggggcgt ggcgtgggtt ccgtcccacc cgcgtgtggg gacggaaccc
     5521 cgccgtcact gggcaatgat ctcgccccgg cttggtcgtc atagccaagc gatctgcttc
     5581 tcccgacctc caaggttctc agctctctca ggaatacgag ggggccttcg gctgtttttg
     5641 tatccaaggg ccaaggattt gcccccttcc gctggggaag gtattccctt accatggtcg
     5701 aacagattca agtagcaggc tccgagcctt cggttttgag gacggacggc gcccggcccc
     5761 gccgcatagc ggcatgccac caggcaaaac gccggccgac cgactcgaaa cacggcaggc
     5821 gtggcgcggt gtccgctttg tcttcgcacg agagaggttt ggaatagatg agtcaaatgt
     5881 cgcgtcccta cgagtccggt cgtgaggaag ccgctcacga ggatctcgtt cggcaatatc
     5941 tgcgcgagat cggtcaatac cccctcctga ctgacgttca agaggtcgag ctcgcccgcg
     6001 ccatcgaaga gggcgagaag gccaacgaga aactccagaa ggccaccaag aagatgaccg
     6061 acaagcagcg cttctcgctg cagtccacag ttcgcacggg tcgggcggcc aagcgtcgct
     6121 tcatccagtc gaaccttcga ctcgtcgtct cgatcgcgaa gaagtactcg gctgcaggtc
     6181 ttcctctgct cgacctgatc caggagggca acctcgggct catgcgtgcc gtcgagaagt
     6241 tcgattacca gcgtggtttc aagttctcca cctacgcaac ctggtggatc cgtcaggcga
     6301 tcacgcgagc gatcgcggac aaggctcgca cgatccgtat cccagtacac atggtcgaga
     6361 cgctctaccg ggttcgcaag gtccaaagtg aactgctcga gaagctcggc cgtgagccga
     6421 cgatcgagga gatcgccgag ggctcggatc tctcgcccga gaaggtgcgt gaggcattcc
     6481 gcgtcctgcc cgagccggtt tcgttgcacg agccggtcgg tgacgaggat gccgaactgg
     6541 gtgacttcat cgaggacgag gatgtcgagg gtcccttcca ggcggccgca gtagcgcttc
     6601 gccaggagga cctgtgggac atgctcggct cactgtcgga tcgcgagcgc aaggtgctcg
     6661 cgcttcgctt cggtctcgtg accggcgagc ctcgcaccct cgaagaggtg ggcaaagagt
     6721 tcaacctgac gcgtgagcgg atccgtcaga tcgaggccaa ggcgctgtcg aagctgcgtc
     6781 acccctcgac tccgggcaag ctccgcgaca tggtcaccat ctaactagcg acttcgggcg
     6841 gcggcgcacg acccgctgtt ccaaaccgtt ctgaatcact gcggacgtac gccgccgtcc
     6901 tcgtcgtcac tagtacgctg tgagcatgat cactaccgtt gttctcgcca cgtgcgatat
     6961 ccatcagatc cctgagacgg ctcagcagat cgcggatatc gaggaggtct ccgaggtcta
     7021 ctcggtcgcc ggtgacttcg acctcgtgat catggtgcgg gtcaagacgc acgacgactt
     7081 ggcccgagtg gtgtccgagc agatcgcaag gatcgccggc atcgagcgga cccagacact
     7141 cgtcgccttc aaggtcttct cccggcatga cgtcgaagca ttgttctcga tcggattcga
     7201 ggaggaaccc gcctcctagc ggggaccgcc gtggacgcgc tgatcgcgca ctaccacgag
     7261 atcggtctca agggccgtaa ccgtgacttc ttcgagcgcg cgctcacgcg caatctgaaa
     7321 cgagcgttgc gcggaaccgg ctactcgcgc gtccgccata tcgccggccg ggtcctggtc
     7381 gatctcgacg acgagacact ggcccaagag gccgcgctac gagcttcccg tgttttcggg
     7441 cttgcgtatg tgggtgcggg cgtccgtgtc gagccaaaca tcgatgagat cggccgcgtc
     7501 gctctcgaac tcatgcaggc agagcccttc gagtcattcc gggtccgggc ccgccgctct
     7561 tatagctcga tgacggagac atctagcgag atcaacaatg tcgtcggaca acagatcaag
     7621 gatgcgaccg agggccgcgt cgacctcaag cacgcggacg cgaccgcgtg gatcgagatg
     7681 ttcggcaaga ccgggatcgt ctatcgcaat aggttggagg ggccgggtgg gttgccggca
     7741 ggaacgtcgg gtcggatgtt ggcgctcctt tcgggaggca tcgactcgcc cgtggccgcg
     7801 tggcggatgg cgcatagggg ggccgaagtc gagatggtcc acttccacgg tcagccattc
     7861 accgatccgt cttccgtaag gcaatcgctc gaactcgctg aagttctcac gcgttaccag
     7921 cttcaaacga tcgtccacct cgtgccgtta tccgacatcc agcgcgagat cgtcacgcat
     7981 gctccggcga acctaagggt ggtgctgtac cgccggacga tgatgcggat cgcggccgag
     8041 cttgcgcttc aaagagatgc cgtcgccctg atcaccggcg attcgctggg tcaggtcgcc
     8101 tcgcagacga tcgagaacat caccacggtc gactcggcca tctccggagt ccaggtgttg
     8161 cgaccgctca tcgggatgga caagcaagag atcgtcaacg aagcgcgcaa gatcgggacc
     8221 tacgagatct cgacgcgcga ctaccaggac tgctgcgtgc tgttccagcc gcgctcgccg
     8281 gcgacgcggg cgaatccggg cttggccgaa cgggccgaga ccgagctgaa tgtggacgcc
     8341 ttagtgggca aggctctggc cgggatcgag acgaggatcc tggagttgcc gcctccctag
     8401 agccgagtct tcgccgactt cgagaagttc cccagcaccg aaacgaccgc accgatcttc
     8461 tcgccgacgt cgcgcgagcc gaatagccgg gccattgagc tgaacaggcc cgtcgtcgtt
     8521 tcgtcgtagg gataccagaa cggcttcttg cgacccggca tcaagctgtg cgcgatgtga
     8581 gtcggcaccg tgaactcgcg gatgccgagc tcgccgctca accgaccgaa gccggattcg
     8641 ccgacgccac cccaaggagc ccatggcatc cccgggagtg ttccgtggtc gttggagccg
     8701 atcccgccgg ccttcacctt ggtcgcgaca tcgtccgcac gcttgcgatc cttcgtccac
     8761 accgacgcgg tgaggttgaa gctctcgttc gctcgacgga tcgcctcttc ttggtcttgc
     8821 actcgagtga tcgtgaggat cgggccgaag ttctcttctt tcacgatgtc catctcttcg
     8881 gacgcgccct tgacgaccgt cggcgcgaac cacaggcttc cgccttcgtt ggtgcggccc
     8941 tcttcgccac ccgccaggat gttggcgccc ttcttcttcg cgtccgcgac ctgcccgacg
     9001 atcttgtcga actggccttt gaacgtgagt gaaccgacgt cgccgtcctc gcccccttgc
     9061 cggagcagct ggagcttgga aacaagcttg tcctcgaaat cgtcggccac cgaatcgacc
     9121 acgaatgcgc gttcgatcga acaacaagtc tgacctgcgt tgaagaacgc tccccacaga
     9181 atgccggagc ttgcgacgtc gatgtcggca tcctcgagaa cgatcgccgc gtccttgccc
     9241 cctagctcca tcacgaccgg ggtgaggtgt ttcgccgcgg cctcacagat cttgcgaccc
     9301 gtcgcaggcg aaccgatgaa cgagatcttg tcgcagggag catcgacgag ggcagcaccg
     9361 acgtctcccc caccctgcat cacgttcgcg actccgggag gaagcggctc gaggatctgg
     9421 cgcatcagct cgccgcacgc aggagtcact tccgacggct tgatgacgac cacgttgccg
     9481 gcgaacagag ccggagcaaa acccaagaag cagttggtga tcgggtagtt ccacggggtg
     9541 atcgccccga cgaccccgta ggggcgccag tcgatgcgcg acgaggtgcc gaacagcagc
     9601 ggccccatga tgcccccgac cttctgcgaa cgaagcgcct tgggggccgt cctctcgaga
     9661 tgggacagca tcagcatcgg aggaacgatg tcatgtgcca gagcctcggc ccgtggcttg
     9721 ccggcttcgg ccgcgaccgt ctcgacgatg tcgtccatga ggtcgtagat gcgatagcgg
     9781 acctctttga ggatgttcgc ccggccctgg ggcgcgatag ccgcccactc gggttgaacc
     9841 ttccgagcct gagcaacgat gtctgcaacc tctcccggat tggtcgcggg gatctcgcgc
     9901 atgacctcgc cggtgcgggg attgaacgac ttgagactct ggccgttcga cgatgtcttg
     9961 gccttgcggg tgcgggtcgt tgccatcacg aacctccgat tcgttgccgg tccaaccaga
    10021 ctaccgacaa aacggacgcc gccccctatg ggagcggcgt ccgggtgcca gtggaggaga
    10081 cttctggctt gaactatttc tcgatcggta ctcccacgag gctgccgtat tcggtccacg
    10141 agccgtcgta gttgcgcacg ttctggaagc ccagcagctc cctcagcacg aaccacgagt
    10201 gggatgaccg ctcgccgatg cggcaatagg cgacgacttc cttgtcagaa gtgatgccct
    10261 cagcggcgta gagcttctcg agctcgtcgg ccgtcttgaa ggtgccgtct tcgttcactg
    10321 tcttggacca ggtgatgttg cttgccccgg ggatgtgccc cggaacctga gcctgctcct
    10381 gcgggaggtg cgggggtgcc agcagctcgc cccggaactc ttcgggagaa cgcacgtcga
    10441 cccacgcctc gttgcccttc ttggcccgct cgatcgcctc ctcgcggaag acacggagtt
    10501 cacccttctc gtctccgagc gtgaagttcg aagccgatgg gctcggtacg tcctgggtga
    10561 gcaagcggct ctcgagctcc cacttcttgc ggcccccgtt aaggagcctc acgtcgtcga
    10621 cgccccggta cttgaacaac cagtaggcgt aggcggcgaa ccagttgttg ttgcccgaat
    10681 agagaacgat cgtctggtcg ctgctgatcc ccttcttgcc gaacagctct gcgagttgct
    10741 cgcgagatac gaactctcgc ctgggggagt cgtgcagctc gttcgcccag tcgatcgaga
    10801 tcgcgttggg gatgtgcccc ttctcgtatg cggttatgtc ctcgtcgcat tcgacgatcg
    10861 ctatgtcgga gtcgttggcg tgctcttcca gccatttcgt gtcgacaagt gcgttggggt
    10921 tggagtaatc ggccacttgg tgctacctcc ctttctctgc tttcagcttc ttggtctctt
    10981 cgatcaactc tttgactcgt gccagttggt gcgtcgcatc ggccagcatc cgttcgagga
    11041 ccctgacctc gaccccgacc tcgtcgaccg tttgggcgac tcggtcggtg ggaacgtcga
    11101 ccccggcttc ggacagattc cctgcctcgc cctcccaggt gaaatagacc ttggccggcc
    11161 tgcccttgct cctgcggcgg gtctcggtgg cgaggaatcc ggcttccacc atgagatcga
    11221 ggtgggcccg ggctacattg gggtggatct gaaagtgatc ggctatctcc tttacggtct
    11281 tggccccgct acgaaggagc aaagccaaga tcgccgcccg ggtgggtagc gacaggactt
    11341 tcgcggtcct ggtagcggaa tcgtctccga ggtccggcat tccagcggca atcctacaga
    11401 ctttggagta tttagcaagt gctaatagtg ctaatgcggt cc
//
LOCUS       DATMED010000359         4136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3748618, whole genome shotgun sequence.
ACCESSION   DATMED010000359 DATMED010000000
VERSION     DATMED010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3748618"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VNP73_09345"
     CDS             <1..328
                     /locus_tag="VNP73_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66164.1"
                     /translation="RSAGKAVDGSLTQKLSPDALALFTELGVEDVIGGRIGGMGGLDS
                     EAEADEIFRRLKLFSLDRKIKSQRTILQELNPLDDADKHDALFTELVGLEAQRRDLLR
                     TIQGAA"
     gene            328..1563
                     /gene="rpoD"
                     /locus_tag="VNP73_09350"
     CDS             328..1563
                     /gene="rpoD"
                     /locus_tag="VNP73_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWL66165.1"
                     /translation="MPDQAAAPPQGPVLTPVSPSQAEVDKILKEAQSDGQITAADLAE
                     RLAVLDLTPEETDAIYHKLVELGVEVVEDESIKEEDDGEDRAPDADEDRVKARKEVDQ
                     ALKAPTNDPVRMYLKEIGRVALLTAQEEVDLAMRIEAGLLAEDQLEKLEKDGQLTDDK
                     RRDLSWTRRDGQMAKRHLVEANLRLVVSIAKRYVGRGMAFLDLIQEGNLGLIRAVEKF
                     DYAKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDLGRE
                     PTAEEIAQQMELPPDKVREIQKISQEPVSLETPVGEEEDSNLGDFIEDADAPVPLERA
                     SFRLLQEQLETVLHTLSEREKEVIRLRFGLVDGQPRTLEDVGKKFGVTRERIRQIESK
                     TLSKLRHPSRSQKLRDYLE"
     gene            1606..2391
                     /locus_tag="VNP73_09355"
     CDS             1606..2391
                     /locus_tag="VNP73_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL66166.1"
                     /translation="MMPTKNRHEPGEFCWAELATTDIEGAKKFYPDLFGWQVNEMEIP
                     GGGSYVIWQLGDSQVAGGMQQGPDEVGKIPPHWNLYVQVEDAEQSAKQAESLGGTIHA
                     PAFDVMEVGRMAVIGDPTGAFLGLWEPKVDRPHIHNENNTVVWNELMTPDTDKAGAFY
                     SELFGWDLKPFEGGNSPYTVFELDGNGVGGMMTTPMEGVPPVWAVYFATEDVDGVVDK
                     MKGAGGQVFMGPEDIPSVGRIAMGTDPQNSAFGVLKPDPSGQQ"
     gene            complement(2453..2719)
                     /locus_tag="VNP73_09360"
     CDS             complement(2453..2719)
                     /locus_tag="VNP73_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66167.1"
                     /translation="MRKIGFLVGFGAGYVLGAKAGTERYDQLKRLYENLASSPQFRQA
                     TNKAKGAAQSGFESAKDLATEGVDKIKSRGDDRPSGLSVAPPPS"
     gene            2849..2921
                     /locus_tag="VNP73_09365"
     tRNA            2849..2921
                     /locus_tag="VNP73_09365"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2882..2884,aa:Asn,seq:gtt)
     gene            2934..3872
                     /locus_tag="VNP73_09370"
     CDS             2934..3872
                     /locus_tag="VNP73_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HWL66168.1"
                     /translation="MGTFTISIDTELAWGYCDRLITPADRREIELERDVIARLLELFE
                     RYRISATWAVVGHLLLEGCERDGEHWHPEIPRPVDSSGRDWFWQHPEERDDRLWYARD
                     VARKIGEADPPQEIASHSFAHVLFDESDTRAEAVAADLREARKVHEANEIEFKSFVFP
                     RNVVGFRRLLAESGVRVYRGSDGHAPSPVAIARARRLAAFLLPTSARLVRPRIDELGL
                     VDVPGSMLLIGRNGLRNIVPPSFVKRKAIKALDASAAADEVFHLWFHPSNLVHGSQTQ
                     FEILDAILRHADGLRSGGTLQILTMGQVADRTLERK"
     gene            3877..>4136
                     /locus_tag="VNP73_09375"
     CDS             3877..>4136
                     /locus_tag="VNP73_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWL66169.1"
                     /translation="MHIADSHDLIRVHGARENNLKEVSVEIPKRRLTVFTGVSGSGKS
                     SLVFDTIAAESQRMINETYSAFVQGFMPNLPRPDVDLLDGLTT"
BASE COUNT          828 a         1229 c         1330 g          749 t
ORIGIN      
        1 tcgatctgct gggaaggccg tcgacggttc gctcacccag aagctgtctc ctgacgctct
       61 tgccttgttc accgagctgg gggtcgagga tgttatcggc ggcaggatcg ggggcatggg
      121 tggtctggat tccgaggcgg aagcagatga gatcttcagg cgtctgaagc tgttcagtct
      181 cgataggaag atcaagtccc agaggacgat ccttcaggaa ctgaatcccc ttgacgatgc
      241 agacaagcac gatgccctat tcaccgaatt ggtcggtctc gaggctcagc gccgagacct
      301 tctccgaacg atccaaggcg ccgcctgatg cctgaccagg cggccgctcc gccccagggc
      361 cccgtgctca caccggtttc tccctcccag gccgaggtcg acaagatcct caaagaggca
      421 cagtcggatg gccagatcac ggccgcagat ctcgccgagc gtctggccgt cctcgatctg
      481 accccagagg aaacggacgc catctatcac aagctcgtcg agttgggcgt cgaggtcgtc
      541 gaagacgaat cgatcaagga agaggacgat ggagaggacc gggctcccga cgcagacgag
      601 gatcgcgtca aagcccggaa ggaagtcgac caggcattaa aggcaccgac gaacgatccg
      661 gtccggatgt acctgaagga gatcggacgc gtcgctctcc tcaccgccca ggaagaagtc
      721 gacctggcca tgaggatcga agccggcctt ctcgccgagg accaactcga gaagctcgag
      781 aaggacgggc agctgacgga cgacaagcgc cgggatctct cctggacgcg ccgcgacggg
      841 cagatggcca agaggcacct ggtcgaggcg aacctgcgac tggtcgtgtc gatcgcaaag
      901 cggtatgtgg gccgcgggat ggccttcttg gatctgatcc aggaggggaa cctgggattg
      961 atccgtgccg tcgagaagtt cgactacgcg aagggattca agttctcgac gtatgcaacg
     1021 tggtggatcc ggcaggccat cacccgggcg atcgccgatc aagcgcgaac gatccggatc
     1081 cccgttcaca tggtcgagac gatcaacaaa ctgatccgga tccagcgaca gctgttgcag
     1141 gacctgggtc gcgagccaac cgcagaagag atcgcgcagc agatggagct tcctcccgac
     1201 aaggtgcgcg agatacagaa gatcagccag gagccggtgt cgctcgagac gccggtgggc
     1261 gaagaagagg actcgaatct tggcgacttc atcgaagacg ccgatgctcc ggttccgctc
     1321 gaacgtgcgt cgttccgttt gcttcaggag cagctcgaga ccgtgcttca cactctctcc
     1381 gagcgcgaga aggaagtcat acggctgcgg ttcggtctcg tcgacggtca accccgcaca
     1441 ctggaagatg tcggcaagaa gttcggcgtc acgcgcgagc gcatcaggca gatcgaatcc
     1501 aagacgctgt ccaagctccg ccatccctct cgcagccaaa agctccgcga ctacctcgag
     1561 tagtagggtc ggccgaacga cctggagacc gttcaaagga ggccgatgat gcccaccaaa
     1621 aaccgtcacg agccgggtga attctgctgg gccgagctcg caactacaga catcgaggga
     1681 gcgaagaagt tctatccaga cctgttcggc tggcaagtga acgagatgga gatccccggt
     1741 gggggctcct atgtcatctg gcagctggga gattcgcagg tagcgggcgg gatgcagcag
     1801 ggacccgacg aggtcggcaa gatcccaccg cattggaacc tctacgttca ggtcgaagac
     1861 gcggagcaat ccgcgaagca ggccgagtca ctgggcggaa cgatccatgc gccggcattc
     1921 gacgtcatgg aagtcggccg gatggcggtg atcggtgatc cgactggggc tttcctcggc
     1981 ttgtgggagc cgaaggtcga tcgaccgcac atccataacg agaacaacac ggtcgtatgg
     2041 aacgagctga tgactcccga taccgacaag gccggcgcgt tctattccga gttgttcggg
     2101 tgggacctga aaccgttcga ggggggcaac tcgccgtaca cggtcttcga gttagacggg
     2161 aatggcgtcg gcgggatgat gacgacgccg atggaaggcg tcccgccggt ctgggccgtc
     2221 tatttcgcca cggaggatgt cgatggcgtg gtcgacaaga tgaagggtgc cggcggccag
     2281 gtcttcatgg gacccgaaga catcccgagc gtaggcagga tcgcgatggg gaccgatccg
     2341 cagaactccg cgtttggagt gctcaaaccg gacccgtccg gacagcaata gaacgagcgc
     2401 aaacaaaagg cccgggcgtc ttgcccgggc cttttcctaa caaacgttgc ttctacgaag
     2461 gagggggcgc gaccgaaagt cccgagggtc tgtcgtcgcc gcggctcttt atcttgtcga
     2521 ccccttcagt agcgaggtcc ttcgcgcttt cgaaccccga ctgggccgct cccttggcct
     2581 tgttcgtggc ctgacggaat tgcggtgacg aagcgagatt ttcgtagagc ctcttcaatt
     2641 ggtcgtagcg ctcggttccg gctttcgccc cgagcacgta tccggcaccg aacccgacca
     2701 ggaacccgat cttcctcatc tttgccctcc caggcttgat cgctttcttg gtgatggtat
     2761 tgcccccttt ctatatggcc caatctcagg tcttagactg gcccgccgcc gggtcgagtt
     2821 cgggctagga atggccggcg ggaagcattc cggggtagct caattggcag agcatccggc
     2881 tgttaaccgg agggttgcgg gttcgagtcc cgcccccgga gcaagatagg cgcatgggca
     2941 cgttcacgat ctcgatcgac accgaactgg cgtgggggta ttgcgaccgg ctcatcacgc
     3001 ctgccgatcg gcgggagatc gagctcgaac gggacgtgat cgcccggctt ctcgagttgt
     3061 tcgagcggta tcggatctcg gccacctggg cggtggtggg gcacctctta ctcgaggggt
     3121 gtgagaggga cggtgagcac tggcacccgg agataccgcg gcccgtcgat tccagtgggc
     3181 gtgactggtt ctggcagcat cccgaagaac gggacgaccg cctttggtac gcccgggatg
     3241 tcgctcgaaa gatcggtgag gcggatccac cacaagagat agcgagtcat tcgttcgctc
     3301 acgtcctgtt cgatgaatcg gatacgagag ccgaggcggt tgcagccgat cttcgagagg
     3361 caaggaaagt ccacgaggcg aacgagatcg aattcaagag tttcgtgttc ccgcgcaatg
     3421 tcgtcggctt ccgccggctc cttgccgagt cgggtgttcg tgtttaccgg gggagcgacg
     3481 ggcatgcacc ttcacccgtg gcgatcgcgc gcgctcgcag gctggcggcc tttctgctcc
     3541 cgacgagtgc tcgcctcgtt cgtccgcgga tcgatgagtt ggggctggtc gatgtgcccg
     3601 gaagcatgct attgatcggc cgcaatggtc ttcggaatat cgtgcccccc tcgttcgtca
     3661 agcgaaaggc gatcaaggct ttggacgcca gcgccgcggc cgacgaggtc ttccacctgt
     3721 ggttccatcc atcgaacctc gttcacgggt cccagactca gttcgagatt ctcgacgcga
     3781 tactccgaca cgcggatggc cttcgttccg gcgggacgct ccagatcctg acgatgggcc
     3841 aggtggccga ccgcaccctc gagaggaagt gaccccatgc acatcgccga cagtcacgat
     3901 ctgatccgcg tgcacggcgc gcgcgagaac aacctcaagg aagtcagcgt cgagatcccg
     3961 aagcgccggc tgacggtctt caccggcgtc tccggctcgg gcaagagctc tctggtgttc
     4021 gacacgatcg ccgcggagtc ccagcggatg atcaacgaga cctacagcgc cttcgtgcag
     4081 ggcttcatgc cgaacctgcc ccgcccggac gtcgacctcc tggacgggct gacgac
//
LOCUS       DATMED010000360         5717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_303226, whole genome shotgun sequence.
ACCESSION   DATMED010000360 DATMED010000000
VERSION     DATMED010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303226"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VNP73_09380"
     CDS             complement(<1..1298)
                     /locus_tag="VNP73_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66170.1"
                     /translation="MRATTRLRSAGPILAFAVLASLFASTSAYGGPDPAARGRYSVDK
                     IDYDGGYTTLSDPNGVPISEQLKGVIHLPVAPDGPWPLIVLLHGRSTTCRYLDQAESF
                     SNPCPEAPPVMSEVKSYRGYDYLGSSLASHGYVVASISANAINSYDIALLASDEGTQW
                     RAQLVVRTLDLLSEWNESGSEELGERLIGTMDFDRIGLMGHSRGGEGVARFVTYIRDR
                     TDGSTYPGLRAVFALAPTDIFDQKVSGVHFGTLLPLCDGDVYTLHGARMFDNARFLEP
                     ERFARVQFTVNGANHNYFNTIWTFDDGPNAGDNSGTNPACDPDDVGSVRISAEEQRDV
                     GLVLMAAFLRRYVGNEMQFDALMTGEASLPAAACPDGARSCPNLVGTSYVGPAADRRM
                     LLLPDGPNGFEASGFEDVAECQPNRDGVGCPTAPNRSLAR"
     gene            1756..2118
                     /locus_tag="VNP73_09385"
     CDS             1756..2118
                     /locus_tag="VNP73_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWL66171.1"
                     /translation="MEYLLMIYSDESQRAEMTPEEMEVGLKGWFDYTDWLREKGWHRA
                     GEALTPTASATTIREKNGEILTTDGPFAETREQLGGYYLVDCANLDEAIEASSRMPNL
                     PRGGSVEIRAIQKFDDAG"
     gene            2124..3365
                     /locus_tag="VNP73_09390"
     CDS             2124..3365
                     /locus_tag="VNP73_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWL66172.1"
                     /translation="MGTQTDQVVDRLFREEQGKAVAILIRVLGDFDLAEDAVQEAFAE
                     ALRSWPTRGVPTNPAAWITTTARNRAIDRLRRERNLQEKAATLTRLAELEAMGEDEED
                     VSGIPDERLRLIFTCCHPALPLEARVALTLKTLGGLTTSEIARAFMVAEPTMAKRIVR
                     AKTKIREAGIPYRVPPLELLPERLPGVLAVLYLVFTEGYSATSGSLLRVDLCDEAIRL
                     ARVVVELMPGEADAAGLLALMLLHHSRRETRASAEGELVLLEEQDRSRWDHDMIDEGL
                     AVLDRALGAGVPGPYQVQGAIAALHARAPRPEDTDWSQIAGLYAVLYRMQPSPVVELN
                     RVVALAMADGPITALPLLDALEERLNRYHLFHSTKADLLRRLDRLEDAAASYRRALDL
                     ATNPYERKFLERRITDVTSGS"
     gene            3418..5025
                     /locus_tag="VNP73_09395"
     CDS             3418..5025
                     /locus_tag="VNP73_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL66173.1"
                     /translation="MTYSDESQRAEMTPEEMEVALKGWILFSLCLAALIINIDVTIVN
                     VALPSLVRQLDASITDLQWVVDAYTLVFAALILAAGSLSDRIGRKGMLLAGLGIFAGG
                     SLAGALVTTPEGLIASRAVMGIGAAGIFPSTLSIIANVFTERSARAKAIGIWGATTGV
                     GIAAGPIVGGWLLEHFWWGIVFLFMVPVAAVTAAFIAAFVPTSRDASAPPLDRRGFAL
                     STAGMGLLILGIIQAPHWGWGSAETVGTVGAGIAILAVFVAVERRTARPMLDVTLFKN
                     LRFTAASGSIAFGFFTLAGFTFMITQYFQFVKSYAPFETGLRILPVALSVGAAAIIGT
                     RLAVKIGNKAVVATGLLLFGGTLQWIAAIAAESTSYGIIALQMVLGGSGLGLITAPAT
                     EAIMGAVAREKAGVGSAVNDATRLFGAALGVAVIGSIAASLYASRLGASVPVGIPPDA
                     LAAARSSVGGGLIAAQQLAGAGLTAAGQGLGAASIGAFLHSLAGALHVAGGVAIAGSI
                     MAAAFLPARPGVHDHAEVSVASPEILN"
BASE COUNT         1024 a         1815 c         1848 g         1030 t
ORIGIN      
        1 tgacgtgcca gcgatcgatt gggagccgtc gggcaaccga caccatcgcg gttcggctga
       61 cactcggcga catcctcgaa gccactggcc tcgaatccgt tgggtccgtc cggaagcaag
      121 agcattcgcc tgtccgccgc cggaccgaca tacgaggtac cgacgagatt cgggcatgac
      181 ctagccccat caggacatgc ggccgccggc agactcgctt cgccggtcat cagggcgtcg
      241 aactgcatct cgttgccgac atacctgcgg aggaacgccg ccatcaggac gagacccacg
      301 tcgcgttgtt cttccgctga tatgcgaacg ctgccaacat cgtcggggtc gcaggcgggg
      361 ttggtaccgc tgttgtctcc tgcgttcgga ccgtcgtcga aggtccagat cgtgttgaag
      421 tagttgtggt tcgcaccgtt gaccgtgaac tgcacgcgcg caaacctctc cggctcaagg
      481 aagcgggcgt tgtcaaacat ccgtgccccg tggagggtgt agacgtcgcc gtcgcacaat
      541 ggcagcagcg ttccgaaatg aactcctgac actttctgat cgaagatatc cgtcggcgcg
      601 agcgcgaaca cggcgcggag tcccgggtag gtcgacccgt cggtgcgatc gcggatgtaa
      661 gtaacgaacc gagcgacccc ctcgcctcct cgcgagtgtc ccatcaggcc gatgcggtcg
      721 aaatccatcg tcccgatgag cctttctccg agctcctccg acccggactc gttccactcg
      781 ctcagaagat cgagtgttcg aaccacgagt tgggcgcgcc actgcgtccc ctcgtccgag
      841 gccaaaaggg cgatgtcgta ggagttgatc gcgttggcgc tgatcgacgc gacgacataa
      901 ccgtggctcg caaggctcga gcccagatag tcgtagcccc gatagctctt gacctcggac
      961 atgacgggtg gcgcctcggg gcacggattg ctgaaggact ccgcctggtc gagatagcgg
     1021 cacgtcgtgc tgcggccgtg cagcagcacg atgagaggcc acggtccatc cggcgcgacg
     1081 ggcagatgga taacgccctt tagttgctcc gagatcggga ctccgttcgg atcgctgagg
     1141 gtcgtatatc cgccgtcgta gtcgatcttg tcgacggaat agcggcctct cgccgccggg
     1201 tcggggcccc cgtaggcaga ggtcgaggca aacagggacg cgagcacagc gaacgccaag
     1261 atcggtcccg ccgagcgcag tcttgtggtt gcgcgcatgt tgcctccttt agctcttctc
     1321 caagttcgcc tgggccccgc ggattcctgc gccggctggg ggtggagcaa ccaaagccga
     1381 gaagggttgg gaaacgacgc agtagtgaat aggaagctgt agagaagtgg aggcctttgc
     1441 agtcgggagt ttttcttctt aagtggacca gagaagattc gaactcctga cctcacccgt
     1501 gcgagcggga tctgcaaaat caaccgcgtc ggtcaggtga cgaacgaatt atgagtccgt
     1561 cttagccaag atctcctgtc gatgtcaggt gtgcttctcg agctcgcata tcccatgcca
     1621 catggacaag agttcacccg ccgaacccac agaactgacc cgaacggctc gttgcgacct
     1681 gtccaaactc gcaactcccg ttcgtcatta gggtgagcgc ggccgggaga ggtcgcccag
     1741 agagaggagg aggccatgga gtacctgctg atgatctact cggacgaaag ccagcgagca
     1801 gagatgacgc cggaagagat ggaagtaggc ctgaagggct ggttcgacta tacggattgg
     1861 ctccgggaga agggctggca ccgggctggg gaggcgctga caccgaccgc ctccgccacc
     1921 acgatccggg agaaaaacgg ggagatcctc acgaccgacg ggcccttcgc cgagaccagg
     1981 gagcagctcg gcggctacta cctggtcgac tgcgcgaatc tcgatgaggc catcgaagcc
     2041 tcgtcgagga tgccgaacct gccacgagga ggatcggtgg agatccgagc catccagaag
     2101 ttcgacgacg cgggatagcg accatcggga cgcagacgga tcaggtcgtc gaccgccttt
     2161 tccgcgagga gcaggggaag gcggttgcga tcctgatccg tgtcctcggg gacttcgatc
     2221 tcgccgagga cgcagtgcag gaagcgttcg cggaggcgct tcgctcctgg ccgacacgcg
     2281 gggtgccgac aaaccccgcg gcgtggatca ccacaacggc ccgaaaccgt gccatcgacc
     2341 ggctgcggcg cgaacgcaac ctccaggaga aggccgcaac gctgaccaga ctggcggagc
     2401 tcgaggcgat gggagaagac gaagaggacg tcagcgggat tcccgatgag cgacttcggc
     2461 tgatcttcac ctgttgccat cccgcgctgc ctcttgaggc tcgggtcgcg ctcacgctca
     2521 aaaccctcgg cggcctgacc acatcggaga tcgccagggc gttcatggtc gccgagccga
     2581 cgatggctaa gaggatcgtc cgggccaaga ccaagatccg agaagccggg atcccatatc
     2641 gggtcccgcc actagagctg ttgcccgagc gtctcccagg tgtcttggca gtgctctatc
     2701 tcgtgttcac cgaggggtac tcggcaacat ccggctccct tctgcgcgtc gatctgtgcg
     2761 acgaggcgat ccgtctcgcg cgtgtcgtcg tggagctcat gccgggagag gccgacgccg
     2821 cgggactgct cgccctaatg ctcctgcacc actcccggcg cgagacccgt gcgtcagccg
     2881 agggtgagct cgtgctactc gaggagcagg atcgctcgcg ctgggaccac gacatgatcg
     2941 acgagggtct cgccgtgctc gatcgagccc tcggtgccgg agtgcccggt ccctatcagg
     3001 tccagggagc gatcgccgcg ctgcatgccc gcgcccccag acccgaggac accgactggt
     3061 cacagatcgc gggcctgtac gcagttctct acaggatgca gccctcaccg gtggtggagc
     3121 tgaatcgggt ggtggccctc gccatggccg acggtcccat caccgcactg cctcttcttg
     3181 acgcgctcga ggaaaggctc aaccgctatc acctgttcca ctcgaccaag gccgacctct
     3241 tgcgccgact cgatcgctta gaggatgccg ccgcaagtta ccgccgcgcg ctcgacctgg
     3301 ccacgaatcc ttacgagcgc aagttcctcg aacgacgaat cactgacgtg acatcgggtt
     3361 cgtaagactc gaccaccagc gcggccggga gaggtcgccc agagagagga ggaggccatg
     3421 acctactcgg atgaaagcca gcgagcagag atgacgccgg aagagatgga agtggccctg
     3481 aagggctgga tcctgttctc cctctgcctc gccgcgctca tcatcaacat cgacgtcacg
     3541 atcgtgaacg ttgcgcttcc gtccctggtc cgccagctcg acgcctcgat caccgatctg
     3601 cagtgggtgg tcgacgccta cacactcgtc ttcgccgctc tcatccttgc ggcgggaagc
     3661 ctcagcgacc gcatcggtcg caagggaatg ctgctggccg ggctcggcat cttcgccggg
     3721 ggaagcctgg ctggcgcgct cgtcaccacg ccggagggat tgatcgcatc gcgcgcggtc
     3781 atgggaatcg gagcggcagg gatcttcccg tcgacgctct caatcatcgc caacgttttc
     3841 accgagcgta gtgcgcgcgc caaggccatt ggcatctggg gagccacgac cggcgtgggc
     3901 atcgccgccg gcccgatcgt cggtggttgg ctgctcgagc atttctggtg gggaatcgtg
     3961 ttcctgttca tggtgcctgt tgccgcggtg accgccgcgt tcatcgccgc cttcgtgccg
     4021 acatcgcgag atgcctcggc gccgccgctc gatcggcgcg gcttcgcgct ctcgaccgcc
     4081 ggcatggggt tgctgatcct cggcatcatc caagcgccgc attgggggtg gggttcggcg
     4141 gagaccgtgg gcacggtagg ggcgggcatc gcgatcctcg ccgtgttcgt ggcggtcgag
     4201 cgacgcaccg cgcgaccgat gctcgacgtg accctcttca agaacctgcg cttcaccgcg
     4261 gcgagcggct cgatcgcatt cggcttcttc actctcgccg gcttcacgtt catgatcacg
     4321 cagtacttcc agttcgtgaa gtcctacgcg ccgttcgaga ccggactgcg gatccttccg
     4381 gttgcgctgt cggtcggggc cgcggcgatc atcggaacgc gactggccgt caagatcggc
     4441 aacaaggcgg tggtcgcaac cggtttgctg ctgttcggcg gcacgctgca gtggatcgca
     4501 gcgatagcgg ctgagtccac gtcctacggg atcatcgcgc tgcagatggt tctaggcggc
     4561 agtggtttag gtctcatcac ggcaccggcg accgaggcaa tcatgggcgc ggtggcacgc
     4621 gagaaagccg gcgtcggctc cgcggtcaac gacgcgacgc gactgttcgg cgccgcgctg
     4681 ggtgttgcgg tgatcggcag catcgccgcc tcgctgtatg cgagccgtct cggcgcatcc
     4741 gtccccgtcg gcatcccgcc cgacgccctg gctgcggcga ggagctctgt cggcggagga
     4801 ctgatcgcgg cgcagcagct cgccggagca ggcctcacgg cagccggaca gggactgggc
     4861 gctgcatcga tcggggcttt cctgcacagt ctcgccggcg cgttgcacgt cgcgggggga
     4921 gttgccatcg caggctcgat catggctgcc gcgttccttc ctgcgcgacc cggtgtccac
     4981 gaccacgcag aggtcagcgt tgcgtcccct gagatcctga attagctcga ccacgggtac
     5041 cctcaaggag tgaagcggct tttttttgtg gcggttccaa gagaggcagg atctagcgga
     5101 atgattgctg ccctctgggc tcggtccaaa gaagacattc agacccagct agccaccgtt
     5161 caagtcttgg atccttcgcc cgattggttg acagatgagc gaaaggaact gatctcctcc
     5221 gtctaccagt acgacattga ttccggactt gacctcaaga ctctgactga gccccccgat
     5281 ctcgttttcg accgggacaa ccccccgccg tggcgtcaag gctgataacc cccagcgtcc
     5341 gagatgagtc ggatccaccg ttagagggag aggcgggccc caacgacagg cccgaccagg
     5401 ggcttcaggg agtggaccag agggttgggg gaagcaacca tctagcacgg gcgtcggcgc
     5461 tgggagctga gcggccgatc atgagtcagc gtattcaccg caacccctga ccttccacaa
     5521 gacgcgatgg cgatgaacgc gtgtcagagg gcgccgaagc gccccctgtt tgctcgagct
     5581 cgttgcttac ctcaggtggt gtcgccgcca cccgtgtctg tccccgcagg cgcgtcgtag
     5641 gtgacgtcgc cggtgttatc gccaccgttc gttccgtcgt ccccatcggt gccgtcaccg
     5701 tcgccggtgt tatcgcc
//
LOCUS       DATMED010000361         4212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2987464, whole genome shotgun sequence.
ACCESSION   DATMED010000361 DATMED010000000
VERSION     DATMED010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987464"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VNP73_09400"
     CDS             <1..212
                     /locus_tag="VNP73_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006665054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL66174.1"
                     /translation="DNGIGFDPRQSGRLFEIFQRLHSEAEYAGTGIGLAICKKIVDQH
                     GGRIWCDSRPGTGAAFFFSMPAVTA"
     gene            209..1264
                     /locus_tag="VNP73_09405"
     CDS             209..1264
                     /locus_tag="VNP73_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HWL66175.1"
                     /translation="MITFRSRPVKVLLVEDNEGDAALVSQMLKRSTLVSAEVTQVERV
                     SAARAALHKDPFDCVLLDLGLPDARGLEALRLVQSAAADTAVLVLTGLDDEDVGIEAL
                     QEGAQDFLTKDKVDTEVLGRAILYSVERQRTESAKRYFLDNAAHELRTPMSIISGAAE
                     VLALHKDEVDTERFDELIALIAKHGKRVGHLLNQLLEFTKLDHVSSGDLEAVDIQATV
                     EKALDLAPPPEGKNVDQMLEEDLKATAHPVRLTHVLAHFLNNAYRYGGKTISIAAEAG
                     DERILMSVTDDGPGVPEELIPQLFEPFGRGSLWRPAGSGLGLALCQRMAKSFEGEVNY
                     KPRLPHGARFEVILKQA"
     gene            complement(1261..1659)
                     /gene="msrB"
                     /locus_tag="VNP73_09410"
     CDS             complement(1261..1659)
                     /gene="msrB"
                     /locus_tag="VNP73_09410"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWL66176.1"
                     /translation="MSPKVEKSEREWQDELTPEQFEVLRNKATEAPFTGKYVHTKDDG
                     TYRCAGCGAELFAADTKFDSGSGWPSFTEPMNRANVELVEDRSHGMVRTEVVCGNCGG
                     HLGHMFDDGPRDRGGQRYCINSCSIDFEKT"
     gene            complement(1669..2502)
                     /locus_tag="VNP73_09415"
     CDS             complement(1669..2502)
                     /locus_tag="VNP73_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWL66177.1"
                     /translation="MAALIFGLGAALAYGAADFMGGIASKRAGAVNVVLITQLLGTSV
                     IVMMLPFFPYSGSVGPAIGWGAAAGVGGGAGVLLLYRGLARGRMSVVAPITGVEAAGV
                     PVVFGLAIGERPGVGALIGVGLALIAVALVSTTRTEEAVPGRSGVPEAIGAGIGFGVF
                     FICLAQAGDSAGLWPLIGARASSLVVAGSAAIVSRVRPSTHGAGREIAVASLLDVAAN
                     VFYLIGVKVGLLSLVAVLTSLYPAGTVALARIFLKERLGRIQVVGVGVAAAAVILIGI
                     G"
     gene            2549..2941
                     /locus_tag="VNP73_09420"
     CDS             2549..2941
                     /locus_tag="VNP73_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL66178.1"
                     /translation="MRILLVDDLPDARFVLRKRLEREGKYEIVGEAANGQEAVELTQS
                     LKPDLVIMDFRMPVMDGLEATRVIKESSSEVEVVGFTAFEDPTMREAMLEAGAVANVN
                     KTDLQDLLKLLSEKAARTASAAPPSVEG"
     gene            2968..>4212
                     /locus_tag="VNP73_09425"
     CDS             2968..>4212
                     /locus_tag="VNP73_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL66179.1"
                     /translation="MRFPPTHKLVRLFLALGLAGALIPAAPAGATQPDSGQQRNYPEF
                     TFERVRVEMRDGVNLSVDLWRPVTPKNVRVPVILTLTPYHSLYYALDPRETDLPSGDA
                     RLLVPKGYAYAQADVRGTWNSGGCWNYGGLKERQDGYDLVEWLGTREWSNGKVAMTGG
                     SYDGTTANAAAVENPPHLATIIPVSAISRWWGYAYQQGTRSSYSGESADIDPPSDTPT
                     DFMFAYGFLPPPEPGALTDAEQIAMRWTPCERVEQTLHGYDTEPDYDAFWRQRDYLRL
                     AHKVKVPVLVAHGLLDFNVKTWEGTAWYQALRSEKAMVIGQWPHANPRSYWSEWDGLL
                     ERWLERWLYGVHNGVEDEPSVHVQTNDKQWHTQNDWGDPDLTAFGIEGDTFEYLDDGS
                     LTESEMLRVGSGGADRFVRVEVP"
BASE COUNT          820 a         1360 c         1331 g          701 t
ORIGIN      
        1 aggacaacgg catcggattc gatcccaggc aatccggccg gctgttcgag atcttccagc
       61 gccttcacag cgaggccgag tacgcaggta cggggatagg ccttgcgatc tgcaagaaga
      121 tcgtcgatca gcacggtggc cgcatctggt gcgattcacg cccggggacc ggcgccgcct
      181 tcttcttctc gatgccggcg gtgaccgcgt gatcaccttc cgctcgcgtc cggtcaaggt
      241 cttgctcgtg gaggacaacg aaggcgacgc tgcactcgtg tctcagatgc tcaagcgaag
      301 cacactggta agtgccgagg tgacccaagt cgaaagggtc tcggccgctc gcgctgcgct
      361 ccacaaggat ccgttcgatt gcgttctgct cgatctcgga ctaccagacg cgcgggggct
      421 ggaggctctg cgcctcgttc agagcgcagc ggccgacacc gctgttctcg ttctgacggg
      481 attggacgac gaagacgtcg ggatcgaggc attgcaggag ggcgcacagg atttcctcac
      541 gaaggacaag gtggacacgg aggttctggg cagggcgatc ctttactcgg tagagcggca
      601 gaggaccgag tctgcgaaga ggtacttcct tgacaacgca gcccacgagt tgcgaactcc
      661 gatgtcgatc atctcggggg ccgccgaggt tttggcgcta cacaaagacg aagtcgatac
      721 ggagagattc gatgaactca tcgcactcat cgcgaagcac ggcaaaaggg tggggcatct
      781 gttgaaccaa ctcctcgagt tcacgaagct ggatcatgtt tcctccggcg atctcgaggc
      841 agtcgacatc caggcgaccg tcgagaaagc actcgacctg gccccgccgc cggagggaaa
      901 gaatgtggac cagatgctgg aggaagactt gaaggcgacc gcgcatcccg tccggctcac
      961 tcatgtccta gcccattttt tgaacaacgc ttaccggtac ggagggaaga cgatctcgat
     1021 agctgcggaa gccggggacg aacggatcct catgtcggtg accgacgacg gccccggtgt
     1081 cccggaagag ctgatccccc aactgttcga gcccttcggg cgagggtccc tgtggcgacc
     1141 cgccggatcc ggcttgggac tcgcgctctg tcagcggatg gccaagtcct tcgagggaga
     1201 ggtcaactac aagccgcgcc taccgcatgg agcgcgcttc gaggtgatcc tgaagcaagc
     1261 ctaggttttc tcgaagtcga tggagcacga gttgatgcag tagcgctggc ccccccggtc
     1321 gcgcgggccg tcgtcgaaca tgtggccgag atggcccccg cagttcccgc agacgacttc
     1381 ggtcctcacc atcccgtgcg atcggtcctc gacgagctcg acgttcgcgc gattcatcgg
     1441 ttcggtgaat gaaggccatc cggagccgga gtcgaacttc gtatcggcgg cgaacaactc
     1501 agccccgcac ccggcgcagc gatatgtgcc gtcgtccttc gtgtggacgt acttcccggt
     1561 gaacggagct tccgtcgctt tgttgcgaag tacctcgaac tgctcggggg tcagctcgtc
     1621 ctgccactcg cgctcgctct tctcgacctt gggtgacatc tgcacctcct atccgatgcc
     1681 gatcagtatg acggcagcgg ccgccactcc tactccgacc acctgtatcc gcccgagtcg
     1741 ctccttcaag aagattctgg cgagagcgac ggtgccggcc ggatagagcg aggtcagcac
     1801 agctaccaac gaaaggaggc cgacctttac tccgatcaga tagaagacgt tggccgccac
     1861 gtcgagcaag ctggcgacag cgatctctcg ccccgctccg tgggtcgaag gccgtacccg
     1921 tgacacgatt gccgctgaac cggcgaccac aagcgaactc gcccgagctc cgatcagcgg
     1981 ccacaggccc gccgagtcac ccgcctgagc gagacagatg aagaacaccc cgaaaccgat
     2041 cccggctccg atcgcttcgg ggaccccgga cctccccggc accgcttctt cggtgcgggt
     2101 cgttgaaacc agagcgacgg cgatcagcgc gaggcccacg ccgataaggg cgccgacgcc
     2161 gggcctctct ccgatcgcga gtccgaaaac gaccggtaca cccgcggctt ctacaccggt
     2221 gatcggcgca acgacgctca tccgcccgcg cgcgaggccg cgataaagaa ggagaacccc
     2281 ggccccgcct cccacgccgg cggctgctcc ccacccgatc gccggaccga ccgacccgga
     2341 gtaggggaag aacggcagca tcatgacgat caccgacgtg cccagcagct gcgtgatgag
     2401 gactacgttc accgcacctg cccgcttgga tgcgatgccg cccatgaagt cggctgcgcc
     2461 gtaagccagc gcggccccca ggccgaagat cagcgcagcc acaatgtttc ctctctaaaa
     2521 gcgccgggct tggtttactc ttccgcccgt gaggatcctg ctggtggacg accttcccga
     2581 cgcacgtttc gttctgagga aacggctcga acgggaaggg aagtacgaga tcgtaggcga
     2641 agcggcgaac ggccaggagg ccgtcgagct aacccagagc ctcaagcccg atctcgtgat
     2701 catggacttc aggatgccgg tcatggacgg cctggaggcc acgcgggtca tcaaggagag
     2761 ctcttcggag gtcgaggtcg tcggattcac cgcgttcgag gatccgacca tgagagaggc
     2821 gatgctcgag gcgggcgcgg tcgcgaacgt gaacaaaacg gaccttcagg acctgctgaa
     2881 gctcctgtcc gagaaagccg cccgtacggc ctctgcggcc cccccgtccg tggagggctg
     2941 acgccgcctg cggagaaacc tctccacatg cgcttcccgc ccacccacaa gctggtcaga
     3001 ctcttcctcg cgctcggcct tgccggagca ctcatacccg cggcccccgc cggggcgacc
     3061 cagccggact ccggccagca acggaactac ccggaattca cgttcgagcg ggtgcgggtc
     3121 gagatgcgcg acggagtgaa cctctcggtc gacctctggc ggccggtaac tcctaagaat
     3181 gtgagggttc ccgtcatcct gactctgacg ccctaccact ccctgtacta cgcactcgat
     3241 ccccgggaga cggatctccc ttcgggcgat gcacgcctcc tcgtgccaaa gggctacgcg
     3301 tatgcccagg ccgacgtccg cgggacgtgg aactccggcg ggtgctggaa ctacggggga
     3361 ctcaaagagc gacaggacgg ttacgacctc gtggagtggc tcggcacgcg cgagtggtcc
     3421 aacgggaagg tagcgatgac cggaggttct tacgacggga ctaccgcgaa cgcggcggcc
     3481 gtggagaacc cacctcatct cgcaacgatc atcccggttt cggcgatcag ccgatggtgg
     3541 gggtacgcgt accagcaggg gacccgctcg agctactcgg gcgagtcggc cgacatcgat
     3601 cctccctccg ataccccgac cgacttcatg ttcgcctacg gattcctacc tccgcccgag
     3661 cctggagcac tgaccgacgc cgagcagata gccatgagat ggacgccgtg cgagcgggtc
     3721 gagcaaaccc tccacggcta cgacaccgaa ccggattacg acgccttctg gaggcaacgg
     3781 gactacctcc gccttgccca caaggtgaag gtgcccgtcc tcgtcgctca cgggttgctc
     3841 gatttcaacg tcaagacgtg ggaggggacc gcctggtacc aggcactgag atccgagaag
     3901 gcgatggtca tcggccagtg gcctcacgcg aaccctcgga gctactggtc cgaatgggac
     3961 ggcctgctcg aacgctggct ggagcgctgg ctatacgggg tgcataacgg ggtcgaggac
     4021 gagccttcgg tccacgtgca gacgaacgac aagcagtggc atacccagaa cgactggggc
     4081 gacccggacc tgaccgcgtt cgggatcgaa ggggacacgt tcgagtactt ggacgatggg
     4141 agcctgaccg agtcggagat gttgcgagtc ggttcgggcg gcgccgaccg tttcgtgcga
     4201 gtagaggtgc cg
//
LOCUS       DATMED010000362         3724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2233374, whole genome shotgun sequence.
ACCESSION   DATMED010000362 DATMED010000000
VERSION     DATMED010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233374"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VNP73_09430"
     CDS             <1..337
                     /locus_tag="VNP73_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL66180.1"
                     /translation="TKGLFDAKAFERMKPDAWLVNVARGGLVDTDALVAALQGKQIRG
                     AALDVTDPEPLPAGHPLWGIEDVIITPHVANTADMAIPELMAIVRRNVQRFSKGEELE
                     GLVDVSLGY"
     gene            450..824
                     /locus_tag="VNP73_09435"
     CDS             450..824
                     /locus_tag="VNP73_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWL66181.1"
                     /translation="MVHAGGDAIVSTDSSEKTFAMLAHLTSFSALIGVPLGNILGPLI
                     IWLIKKDEFPLVDDQGKESLNFQISFTIYAIVSAILILVVVGIFMLVALGIFWLVVVI
                     VASVRANEGKQYRYPMTIRFIS"
     gene            complement(814..1326)
                     /locus_tag="VNP73_09440"
     CDS             complement(814..1326)
                     /locus_tag="VNP73_09440"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWL66182.1"
                     /translation="MAGAVVPSGMAAPRYAGWWRRVGAWLIDWCIFLVPSLLLSIPFI
                     LEIFEQIANAQSGPVGVEEIGTAPLALLMVGSLILNVGFIAYEIIMNGGPRGQTVGKM
                     ALSIQVRDADNFGPIGYGKAAVRYFVGYALVAACWIPGIVDCLFPLWDEKKQTIHDKA
                     ANSVVLDVRR"
     gene            complement(1346..1912)
                     /locus_tag="VNP73_09445"
     CDS             complement(1346..1912)
                     /locus_tag="VNP73_09445"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWL66183.1"
                     /translation="MTDPTGGAPPPPPSQPPATPPPAPGAPAAGVAGTYAEWWKRVVA
                     RIIDGVILAIANAILSIPLGGLFAVSQPTFDPTTGEFSGGGGFFATYILANLLIFILN
                     IIYFTAFHAVNNGQTPGKMAMKIAVRGEEDGAPPDWGKAAIRQIVEVVLAFLCFIPAI
                     VDALFPLWDPKKQTIHDKAAKTVVVDVA"
     gene            complement(1935..2300)
                     /locus_tag="VNP73_09450"
     CDS             complement(1935..2300)
                     /locus_tag="VNP73_09450"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HWL66184.1"
                     /translation="MWAGLARRIDLSGFHLQVSAGLLLLGAFLWPLIPGHIGLVCPLR
                     ATTGIPCPLCGMTTSVVATGRGRFSDALAANPGGLVAVAAAVILLLFRPKNVRVHVGL
                     LVVVLTAMWVFELFRFSVL"
     gene            2370..2939
                     /locus_tag="VNP73_09455"
     CDS             2370..2939
                     /locus_tag="VNP73_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66185.1"
                     /translation="MLVLLGACNATPSDPNAAPRVPQTSRTSDRAERKPTKPHPRVIN
                     GAFGFTERRIDRAIADLKRLGLWNRLTDHLYVLDIGSRLGTENVPEDRHLADAYSTGF
                     VDEQDSGQLCDLMFYATAIKRDLRRWRGFYSQGLARHQPLPLGRYWTQLLAHELAHCL
                     PGPHGESVAQAWEARVRRAYERSDAGSKS"
     gene            2972..>3724
                     /locus_tag="VNP73_09460"
     CDS             2972..>3724
                     /locus_tag="VNP73_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL66186.1"
                     /translation="MTTFTRRALIAAALALAVVAVGLVPAQADSAGDTLAARERFFGA
                     ANVDSSGNVRDDRVILSWFSVSSYAASFNGHVVLLDAWIARGSYSDYVPTTTEELIAL
                     APEYIFVGHGDFDHAADLGEVASGTGATIVGSPEHCDSAKEQSGDDSIKCLEVVPAGA
                     PPGIRKELDLLPGVGISVVSHIHSAVESYENDRTPCPPVWNPQDTVNNPPSPEDIEHV
                     TKWNTPRGVNLLYQFRVGRFSLTWHDTTGKIEE"
BASE COUNT          718 a         1166 c         1157 g          683 t
ORIGIN      
        1 aacgaagggc ctgttcgacg caaaggcgtt cgagcggatg aaaccggatg cctggctcgt
       61 caacgttgcc cggggcgggc tcgtcgacac cgacgctctg gtcgccgctt tgcaaggcaa
      121 gcagatccgc ggggcggctc tggatgtaac ggaccccgag ccacttccgg ccggccatcc
      181 tttgtggggc atcgaggacg tgatcatcac tccccacgtt gcgaacaccg ccgacatggc
      241 gatcccggag ctcatggcga tcgttcgcag gaacgtgcag agattctcta aaggtgagga
      301 actggagggt ctcgtggacg tttccctggg atactgaagc catggccgac gagactcccg
      361 tagatccgct tcccggcgac ggtccgcctc aggcggttcc tccggcggcg gctcctccca
      421 cgccttcgat aggcgatatg gggacgggca tggttcacgc gggtggcgac gccatcgtct
      481 cgacggacag ctccgaaaaa acgttcgcga tgcttgctca cctgacgtcg ttcagcgcgc
      541 tgatcggcgt tccgttgggg aacatcctcg gtccattgat catctggttg atcaagaagg
      601 acgagttccc gctcgtcgac gatcagggca aggagtctct gaacttccag atctcgttca
      661 cgatctacgc gatcgtctcg gcgatcctga tcctcgtggt cgtagggatc ttcatgctgg
      721 tcgcgctcgg gatcttctgg ctcgtcgttg tgatcgtcgc ctcggtgcga gcgaatgagg
      781 gaaagcagta ccgctatccc atgacgatca ggttcatctc ctaacgtcca gaacgacgct
      841 gttcgcggcc ttgtcgtgga tcgtttgctt cttctcgtcc catagaggga agaggcagtc
      901 gacgatcccc ggtatccagc aagccgccac caaggcgtaa cccacgaaat agcggacggc
      961 agccttcccg tagccgatcg ggccgaagtt gtctgcatcg cgcacctgga tgctcagagc
     1021 catcttgccg accgtttgcc ctcgcggccc cccgttcatg atgatctcgt acgcgatgaa
     1081 ccccacgttc aggatgagcg agccgaccat gagaagcgcc aggggcgcgg tcccgatctc
     1141 ttcgacgccg accggtccag actgcgcgtt cgcgatctgc tcgaagatct cgaggatgaa
     1201 tgggatcgag aggaggagac tgggaaccag gaagatgcac cagtcgatga gccacgctcc
     1261 gactcgacgc caccagccgg catagcgagg cgccgccatc cccgatggga cgacggcgcc
     1321 tgccatgtga tcgaactctt gctcgttacg cgacgtcgac gactacggtc ttggccgctt
     1381 tgtcgtggat cgtttgcttc ttcggatccc acagcgggaa cagagcgtcc acgatggcgg
     1441 ggatgaagca gaggaatgcc agaaccactt cgacgatctg gcggatcgca gccttgcccc
     1501 agtccggcgg ggccccgtcc tcttctcctc gaaccgcgat cttcatcgcc atcttgcccg
     1561 gcgtctgacc gttgttcacc gcgtggaacg cggtgaaata gatgatgttc aggatgaaga
     1621 tcaggagatt cgccaggatg tatgtggcga agaacccacc accgccgctg aactcacccg
     1681 tcgtgggatc gaacgtgggc tgacttacag cgaagagccc accgagcggg atggaaagga
     1741 tcgcgttcgc gatcgccagg atgaccccgt cgatgatgcg cgctactacg cgcttccacc
     1801 attctgcgta cgtccctgca actcccgcgg ccggcgcacc gggcgccggg ggcggcgttg
     1861 cggggggttg actgggcgga ggagggggtg cccccccggt cggatctgtc attcgttctc
     1921 ctttctcggt tctcctacag gactgaaaaa cggaacagct cgaacaccca catcgccgtc
     1981 agcacgacaa caagcaagcc gacatgaacg cgcacgttct tgggacggaa cagcagcagg
     2041 atgacggccg cggcgacggc caccaacccg ccggggttgg ccgcaagcgc gtccgagaac
     2101 cgaccgcgcc cggttgcaac gacgctggtc gtcatcccgc atagggggca ggggatcccg
     2161 gtggttgcgc gcaaaggaca gacaagtccg atatgcccgg gaatgagagg ccagaggaac
     2221 gcacccagca acagaagtcc ggcggatacc tgaagatgga aaccgctcag gtcgatccga
     2281 cgggcaagcc cagcccacat aatcgagagg ttataagtgc tcccctctac ggtgatagtg
     2341 atttggccct gagatttctt tccatagtca tgcttgtgtt gcttggcgcg tgcaacgcga
     2401 cgccgtcaga tccgaacgcc gcgccccgtg ttccgcagac gtcgcggacc tcagatcggg
     2461 ctgagaggaa gccgaccaaa ccccatccca gagtcatcaa cggagcgttc ggcttcaccg
     2521 aaaggcggat cgatcgagcg atcgcggatc tcaagaggct gggtctgtgg aaccggctca
     2581 cggaccatct ctacgtcctc gatatcggtt cccgactggg gacggagaac gttcccgaag
     2641 accgacatct ggcagatgcc tactcgaccg gcttcgtgga tgagcaggac tccgggcagt
     2701 tatgcgacct gatgttctac gcgaccgcga tcaagcggga cctgcggcgg tggcgcggtt
     2761 tctactcgca gggtctggcc cgccatcaac ccctgcccct gggccgctat tggactcaac
     2821 tcctggccca cgagctggcc cactgcctcc ccggccccca cggcgaatcc gtcgcgcaag
     2881 catgggaggc cagggtccga cgggcctacg agcgatcgga cgccggatct aagtcgtagg
     2941 acatccgggt cgcgcgccga atccaagagg gatgactact ttcacgcgac gcgcccttat
     3001 tgctgcggcc cttgccctcg ccgttgtcgc ggtcggcctc gtgccggcgc aggccgacag
     3061 cgcgggagac acgcttgcgg ctcgagagcg cttcttcggc gcggcaaacg tcgattcgag
     3121 cgggaacgtc agggatgatc gcgtgatcct gtcgtggttc tccgtgtcga gctacgcggc
     3181 gtcgttcaac ggtcacgtcg ttctactgga tgcctggatc gctcgaggct cgtattcgga
     3241 ctacgtgccg acgacgacgg aagagctgat agcgctcgct cccgagtaca tctttgtcgg
     3301 ccacggcgac ttcgatcacg ccgccgacct gggcgaggtg gcttcgggaa ccggcgccac
     3361 gatcgtcggt agtcccgagc actgtgattc ggccaaagaa caatcgggtg acgactcgat
     3421 caagtgtttg gaagtggttc cggccggtgc gccgcccggg atccgaaagg agctagacct
     3481 tcttcccgga gttggaatct cggtcgtaag ccacatccat tcggcggtcg agagctacga
     3541 gaacgaccgc acgccctgcc ctccggtctg gaatccccag gacaccgtca acaacccgcc
     3601 gagtccggaa gacatcgagc acgtgacgaa atggaacacg cctcgagggg tcaatctgct
     3661 ctatcagttc cgcgtcggcc gtttctcact gacatggcac gacacgacgg gcaagatcga
     3721 agag
//
LOCUS       DATMED010000363         4098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4899674, whole genome shotgun sequence.
ACCESSION   DATMED010000363 DATMED010000000
VERSION     DATMED010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4899674"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1567
                     /locus_tag="VNP73_09465"
     CDS             221..1567
                     /locus_tag="VNP73_09465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWL66187.1"
                     /translation="MKALSQTGNQIMQMAAATPDSRERYVDFLRAFSIAVVVFGHWLM
                     AIVYWGQDGLTGDNALNVVPGLWLATWVLQVMPLFFFVGGFSNLVTIDATRKKGGTYG
                     EFLRGRIHRLLKPTLVFVGVWIVVAAAIEAIAPSAATSLANATEILAKPLWFLAVYVL
                     LVALAPSMLSLHRRFGARVLFALAIAAAAADVIRLAAGVTFVGYFNFAFVWLFAHQLG
                     FFYAEGTLARLGRRVHAGMAATGLAALFVLTNIGVYSPSLVGMNSDLASNNSPPTVCL
                     IALTTWLVGAAMLLRPAVSRWLSKTRPWAYVIGANSMIMTVFLWHLTALFALVLVAYP
                     LGFAQPAGGTAEWWALRPVWIGLLLVALVPFIALFGRFERPTRGKTAADVKTISPARV
                     SVGTAAVVFGLSGFATAGFAGVLSAPAQVSALGAGPLVSAISLAAGARFLTGTKAA"
     gene            complement(1564..1764)
                     /locus_tag="VNP73_09470"
     CDS             complement(1564..1764)
                     /locus_tag="VNP73_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66188.1"
                     /translation="MTALIGGSVLLIAGSAIALLFGWITAEAVLIWLSIGASVVAGIL
                     LALGFARSRSEVEGAPRSDDET"
     gene            complement(1790..2617)
                     /locus_tag="VNP73_09475"
     CDS             complement(1790..2617)
                     /locus_tag="VNP73_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWL66189.1"
                     /translation="MELTTQDVQEQQFHDAWRGYSQEEVDDFLDHVAETVERLTRENE
                     ALRARLNELDEAVGSAKATEEMLKQTLMTAQQTAEQSVAEAREQAEKLMAEAEQRVQN
                     AEEEVRRRVEEAEEQRRRRTEEIEDRFEVQKSELSSSLERLQAFEGDIKRRLRAFLEQ
                     QLEALGSLGDGPTPTGVGGSPTESEAAPRAEVVVEEIATPESSPEAEVVDEVHDEEEP
                     VDPAATVEAIRTMFDSLPADEEQDAALDEFKPFDDDQAALQGRRRRGLFRRRVDDWA"
     gene            complement(2676..2924)
                     /locus_tag="VNP73_09480"
     CDS             complement(2676..2924)
                     /locus_tag="VNP73_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWL66190.1"
                     /translation="MEILCLAIYVYWVVLFARILLSWLTMVWSPPPALDPVVRVIYDL
                     TEPVMSLFRRYIPPIGGFDLSPIFIFIILQLISSAICG"
     gene            complement(2932..3396)
                     /locus_tag="VNP73_09485"
     CDS             complement(2932..3396)
                     /locus_tag="VNP73_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HWL66191.1"
                     /translation="MAGVWRKTLIYLGLVEDDEFDEYHYDDLEQEPPPPPPPRRGGPR
                     GGDTGPQSVVRQLPTAPPAMFHLVTPSNFNDAQEVGDKFRDGFSVLMNLAGADAMLRR
                     RLIDFASGLAYGLNGAMQPVADNVFLITPSGVQVSAEERRRFLEERGFFNQA"
     gene            3395..>4098
                     /locus_tag="VNP73_09490"
     CDS             3395..>4098
                     /locus_tag="VNP73_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66192.1"
                     /translation="MPEDYPGPSSERRSRTTTPERRAAWRSTRLSGCGAEDGLAYTDD
                     RGPFLDGHLEIPGHPHRQFGRGRHLDGELLPEVPEFREWLARRGRIVEEGSHGHQAPH
                     LDTVQLGHVARQGDRLSGIDAVLCCISSDVDLDQNGLHHAEIHRSTRDGLAYTEPIHR
                     MDQARGADDLTNLVRLKVADEVPPHAVPNHRVLLLELLRPAFADIGDAGFGRRNDHVG
                     RKCLADRHDVDRVRVTV"
BASE COUNT          666 a         1368 c         1273 g          791 t
ORIGIN      
        1 gaaaacgagg acccctcgag cgagggttcg ccgcgagcgt tggggggccg ggagctggct
       61 actcatgtac ccaggttcgc gcaagtcccc ccttccagaa gtacctaggt gaaatgggta
      121 cccgctagcc cccggatggg gcgatgtccg tctaccgggc cggtcccgcc ctcccgatgc
      181 tggagatgtc ggaaaaacac gtcccagaag gagtaccgag atgaaggctc ttagccagac
      241 cggcaaccag atcatgcaga tggcggccgc cacccccgac tcccgcgagc ggtacgtcga
      301 tttcctccgg gcgttctcga tcgcggtggt cgtgttcggt cactggctga tggcgatcgt
      361 ctactggggg caggacgggc tcacgggcga caacgctctg aacgtggttc cggggctctg
      421 gctggcaacg tgggtgcttc aggtcatgcc cttgttcttc ttcgtcggcg ggttctccaa
      481 tctcgtcacc atcgacgcga cgagaaagaa gggcggcaca tacggtgaat tcctgcgcgg
      541 tcgtatccat cggcttctca aaccgacgct cgtcttcgtc ggcgtgtgga tcgtcgtggc
      601 cgccgcgatc gaagccatcg ctccgagcgc ggcaacgtct ctggcgaacg cgaccgagat
      661 cctggcgaaa ccgctctggt tcctcgccgt gtacgtgttg ctcgttgctc tcgctccgtc
      721 gatgctgtcg cttcaccgtc gcttcggcgc acgcgttctc ttcgcgctgg cgatcgcggc
      781 cgcggccgcc gatgtgatcc gtctcgccgc aggagtgacc ttcgtcggct acttcaactt
      841 cgcgttcgtg tggctgttcg ctcaccagct cggcttcttc tacgccgagg gcacactcgc
      901 ccggctcggc cggcgcgtcc acgccggcat ggctgcaacg ggactagcgg cgttgttcgt
      961 cctgacgaac atcggcgtgt actcacccag cctcgtcggg atgaacagcg acctcgcctc
     1021 gaacaactcg cctccgacgg tgtgcctgat cgcactgacg acatggttgg ttggcgcggc
     1081 catgctgctc cgccctgctg tgagccgctg gctgagcaag acgcgtccgt gggcgtacgt
     1141 gatcggtgcc aattcgatga tcatgaccgt gttcctgtgg cacctgaccg cactgttcgc
     1201 actcgtgctg gttgcctacc cgttggggtt cgcgcagccc gcgggtggaa ccgccgagtg
     1261 gtgggcgctc cgtccggtgt ggatcgggct tctgctggtg gcattggttc cattcatcgc
     1321 actgttcggc cgcttcgaac ggccgacgcg cggcaagacc gcggccgacg ttaagacgat
     1381 ctcccccgca cgtgtgtcgg tcggcacggc agccgtcgtg ttcggtctct cgggattcgc
     1441 tacggccggc ttcgccgggg tgctaagtgc tcccgctcaa gtgtccgctc tcggtgcggg
     1501 cccgctcgtc agtgcaatca gtctcgccgc cggtgcccgt tttttgacgg gcaccaaggc
     1561 ggcctaggtt tcgtcgtccg aacgcggcgc gccttcgacc tccgatcggc tgcgcgcgaa
     1621 gccgagtgca agcaggatcc cggccacgac gctcgcgccg atcgagagcc agatcagaac
     1681 ggcctcggcg gtgatccatc cgaacaagag agcgatcgcc gatcccgcga tgagaaggac
     1741 ggagccgccg atcagggcgg tcatgtgtga ctagtggagc gacggtccac taagcccagt
     1801 cgtcgacccg acgcctgaag agccctcggc gccggcgacc ctgcagcgcc gcctgatcgt
     1861 cgtcgaaggg tttgaactcg tcgagcgccg catcttgttc ctcgtcggcg ggaaggctgt
     1921 cgaacatcgt gcggatcgct tcgacagtgg ccgcggggtc gaccggttcc tcttcgtcgt
     1981 gaacctcgtc gacaacctct gcttcgggcg agctctcggg tgtcgctatc tcctccacaa
     2041 caacttcggc ccgcggcgcc gcctcggact cggtgggcga cccgccgaca ccggtcggtg
     2101 tcggaccgtc cccaagtgat ccgagagctt ctaactgctg ctcgaggaac gcacgcaaac
     2161 gtcgcttgat gtcgccctcg aaggcctgca agcgctccag cgacgaagac agctcggact
     2221 tctggacctc gaatcgatcc tcgatctcct cggtacgcct acgcctctgc tcctcagcct
     2281 cctcgacgcg cctgcggacc tcttcttctg cgttctgcac acgctgttcg gcctccgcca
     2341 tgagcttctc ggcctgttcc cgcgcctccg cgaccgactg ctcggccgtc tgctgtgcgg
     2401 tcatgagtgt ctgcttgagc atctcttcgg tcgccttcgc cgacccgacc gcttcgtcga
     2461 gctcattcaa ccgagcccgc aaagcctcgt tctctcgggt caaccgttcg accgtctcgg
     2521 cgacgtgatc gaggaagtcg tcgacctcct cctggctgta gccgcgccag gcgtcgtgga
     2581 actgctgctc ttgtacgtct tgtgtggtta gttccattgc catccctccc tgcttcgagt
     2641 atcccccacg accgcaacca aaacgcggtg atccgtcaac cgcagatggc ggacgagatc
     2701 agctggagga tgatgaagat gaagatgggt gagaggtcga accccccgat cggcgggatg
     2761 tagcgcctga acagggacat caccggctcg gtcaggtcgt agatcacgcg cacgacgggg
     2821 tccaaggccg gtggagggga ccacaccatc gtgagccacg acaacaggat cctcgcgaag
     2881 aggactaccc aatagacata gatggcaagg cagaggattt ccacttaaga ctcaggcctg
     2941 gttgaagaag cccctctctt cgaggaaccg ccgcctttct tcggccgaga cctgcacgcc
     3001 cgacggggtg atcaaaaaga cgttgtcggc gaccggctgc atcgccccgt tcagtccgta
     3061 ggcgagcccg ctggcgaagt cgatcagtcg ccggcgaagc atcgcgtccg ccccggcgag
     3121 attcatgagc accgagaacc cgtcccggaa cttgtccccg acctcttgag cgtcgttgaa
     3181 gttcgacgga gttacgagat ggaacatcgc cggtggggcc gtcgggagct ggcgaacgac
     3241 gctctgtggc ccggtatccc cacctctcgg cccccctcta cggggaggcg gcggaggggg
     3301 cggttcctgc tcgaggtcgt cgtagtgata ctcgtcgaac tcgtcgtctt cgacgagacc
     3361 gaggtagatg agtgtctttc tccacacgcc tgccatgccg gaagattacc ccggtccgtc
     3421 gtcggaacgc cggagccgta caaccacacc cgaacgtcga gcggcttggc gctcgacgag
     3481 actcagcggt tgcggggccg aagatggcct cgcctacacg gacgatcgtg gccccttcct
     3541 cgacggccac ctcgaaatcc cgggtcatcc ccatcgacag ttcggtcgcg gcaggcatct
     3601 cgacggcgag ctgttgcccg aggtccctga gttccgagaa tggctcgcgc gccgcggtcg
     3661 gatcgtcgaa gaagggagcc atggccatca ggccccgcac ctcgataccg tccagctcgg
     3721 ccacgtggcg cgccagggcg atcgtttgag cgggatcgac gccgtgcttt gctgcatctc
     3781 gagcgatgtt gacctcgatc agaacgggtt gcaccacgcc gagattcacc gctcgacgcg
     3841 cgatggcctc gcctacaccg aaccgatcca ccgaatggat caagctcgag gcgccgacga
     3901 cctgacgaac cttgttcgtc tgaaggtggc cgatgaagtg ccaccgcacg cggtccccaa
     3961 tcaccgcgta cttctccttg aactcctgcg cccggctttc gccgatatcg gtgatgccgg
     4021 cttcggccgc cgcaacgatc acgtcggccg gaaatgtctt gctgaccgcc acgacgttga
     4081 tcgcgtccgg gtcacggt
//
LOCUS       DATMED010000364         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4135902, whole genome shotgun sequence.
ACCESSION   DATMED010000364 DATMED010000000
VERSION     DATMED010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4135902"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /gene="fusA"
                     /locus_tag="VNP73_09495"
     CDS             complement(<1..1864)
                     /gene="fusA"
                     /locus_tag="VNP73_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWL66193.1"
                     /translation="MAHIDAGKTTTTERILYYTGKTYKIGEVHEGGAVMDWMVQEQER
                     GITITSAATTCMWKGYWINIIDTPGHVDFTVEVERSLRVLDGAVAVFDAVAGVEPQTE
                     TVWRQADRYNVPRICFVNKMDRTGADFFRTVSMIEDRLNGTPVVIQIPIGAEADFHGV
                     VDLVTMKALYWPSDGMGEEWETRDIPDELKADADKWHHDMVEILADHDEELMEIYVHE
                     TDPDPDVIKKAMRRGTLAGEITPVLAGSAFKNKAVQPLLDAIALYLPSPLDLPPMIAT
                     RPDSGETVAREPEDHAPFSALAFKIMSDPYVGRLTYLRVYSGTLKTGDHALNSSRDRK
                     ERIGRLLQMHANHREDKEAVFAGDIVAVVGLKHTLTGDTICDPGDPVLLEQMSFPAPV
                     IDVAIEPKSKADSDKLGNGLQRLSEEDPTFRVHTDDETGQTIISGMGELHLEVIVDRL
                     IREFGVGANVGKPQVAYRETIKNPVQKVETRYVKQTGGKGQFGHVVFNVEPTGPGGGY
                     EFINKITGGKIPTEYIPSVDQGVQEAMASGVMAGYPVVDIRVTLIDGGYHEVDSSEMA
                     FKITGSMAFKDAAKKAKPVLLEPIFDIEVVTPEEYMGDVIGDLSGRRGRVEAMDQ"
     gene            complement(1989..2459)
                     /gene="rpsG"
                     /locus_tag="VNP73_09500"
     CDS             complement(1989..2459)
                     /gene="rpsG"
                     /locus_tag="VNP73_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWL66194.1"
                     /translation="MPRKGPAPRRELLPDPVYESLLVTQIQNRIMTRGKRTLSEQIVY
                     DALRKVADKSGKDPLPILKKAIDNIKPLLEVRSRRVGGATYQVPVEVQSRRGTTLAIR
                     WLVNFARQRRERSMADRLAGEVLDASGGVGNAVKRREDMHKMADANRAFAHYRW"
     gene            complement(2463..2837)
                     /gene="rpsL"
                     /locus_tag="VNP73_09505"
     CDS             complement(2463..2837)
                     /gene="rpsL"
                     /locus_tag="VNP73_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWL66195.1"
                     /translation="MPTIQQLVRQGRRSKREKSKSPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSGIEITAYIPGIGHNLQEHSIVLVRGGRVKDLPGVRYKIIRAAL
                     DTAGVRDRVQSRSKYGAKKAGG"
     gene            complement(3278..>3521)
                     /locus_tag="VNP73_09510"
     CDS             complement(3278..>3521)
                     /locus_tag="VNP73_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66196.1"
                     /translation="SAFGEARGAEESFGDLGGAEDALPQDGGGETGGAEEADEAEKAD
                     EAPEAVVTDLPVAEAETPQEPEGEEATPDTEDESPA"
BASE COUNT          559 a         1158 c         1078 g          726 t
ORIGIN      
        1 gctggtccat cgcctcgacg cgaccgcgac gtccggagag gtcaccgatg acgtcaccca
       61 tgtactcctc gggcgtaacg acctcgatgt cgaagatcgg ttcgagcaga accggcttcg
      121 ctttcttcgc cgcgtccttg aaagccatcg atccggtgat cttgaaggcc atctcggacg
      181 agtcgacctc gtggtaaccc ccgtcgatga gcgtgacgcg tatgtccacc accgggtaac
      241 cggccatcac gcccgacgcc attgcctcct gcacaccttg atcgaccgac gggatgtact
      301 cggtcgggat ctttccgccc gtgatcttgt tgatgaactc gtaaccccca ccgggacctg
      361 tcggctcgac gttgaagacg acgtgcccga actggccctt accaccggtc tgcttgacgt
      421 agcgggtctc gactttctgg accgggttct tgatcgtctc ccggtacgcg acctggggct
      481 taccgacgtt cgcacctaca ccgaactcgc ggatcaagcg atccacgatg acttcgaggt
      541 ggagctcgcc catgcccgag atgatcgtct ggcccgtctc atcgtcggtg tggacccgga
      601 aagtggggtc ctcctcggac agacgctgaa gaccgttccc gagcttgtcg gagtctgcct
      661 tcgacttcgg ttcgatagcg acgtcgataa cgggggcggg gaagctcatc tgttcgagaa
      721 gaacggggtc gccggggtcg cagatcgtgt cgcccgtgag ggtgtgcttg agccctacaa
      781 ccgcgacgat gtcgcctgcg aagaccgctt ccttgtcctc acggtggttg gcatgcatct
      841 gcaacagtcg accgatgcgc tccttgcgat cgcgtgacga gttcagcgcg tgatcaccgg
      901 ttttcagcgt tcccgagtag acacgtagat aggtcagtcg gcccacgtaa gggtcgctca
      961 tgatcttgaa ggcgagagcc gagaacggtg cgtgatcctc gggctcgcgc gcgacggttt
     1021 cgccggagtc cggccgtgtg gcgatcatcg gcggcaggtc gagagggctc ggtagataga
     1081 gcgcgatcgc gtccagcagc ggctgcacgg ccttgttctt gaacgccgac cctgccagca
     1141 ccggtgtgat ctccccggca agagtgcccc tgcgcatcgc tttcttgatg acatcgggat
     1201 cgggatccgt ctcgtggacg tagatctcca tgagttcctc gtcgtggtcg gccaggatct
     1261 caaccatgtc gtggtgccat ttgtccgcgt cggctttcag ctcgtcgggg atgtcgcgcg
     1321 tctcccactc ctcgcccatc ccgtctgaag gccagtagag agccttcatc gtcactaggt
     1381 cgacgacgcc gtggaagtcc gcctcggccc cgatcgggat ctggatgacc accggtgttc
     1441 cgttcaaccg atcttcgatc atcgagacgg tcctgaagaa gtcggcaccg gttcgatcca
     1501 tcttgttcac gaagcagatg cgcggcacgt tgtagcggtc ggcctgacgc cacaccgttt
     1561 cggtctgagg ctctacgcct gcaaccgcgt cgaagacggc tacggcgccg tccaaaaccc
     1621 ggagcgaacg ctcgacctcg accgtgaagt cgacgtggcc gggcgtgtcg atgatgttga
     1681 tccagtagcc cttccacatg cacgtggttg cagccgatgt gatcgtgatg ccgcgctctt
     1741 gttcctgaac catccagtcc atgacggcgc cgccctcgtg gacctcgccg atcttgtagg
     1801 tcttaccggt gtaatagagg atccgctcgg tcgtcgtggt cttgcccgcg tcgatgtggg
     1861 ccatgatccc gatgttgcgc gtgtgcgcga gcggatactc gcggatcgcc gggccgccac
     1921 ctttgacggc cttgagattc ggcctttgcg agggatcctg tctctctgct gcttcgggca
     1981 taactagatc accaccggta atgggcgaac gccctgtttg cgtccgccat cttgtgcatg
     2041 tcttcgcggc gcttcaccgc gtttcccacg ccacccgacg cgtcgaggac ctcgccggcc
     2101 agccgatcgg ccatgcttcg ctcacgccgt tgacgcgcga aattgaccag ccaccggatc
     2161 gcaagggtcg tcccgcggcg cgattgaacc tctacgggga cctgatacgt ggccccccca
     2221 acgcgacggg agcgaacctc cagcagcggc ttgatgttgt cgatcgcctt cttgaggatc
     2281 ggaagtgggt ccttgccgga cttgtcggca accttcctta acgcgtcgta aacgatctgc
     2341 tcggagaggg tcctcttgcc tcgcgtcatg atccggttct ggatctgcgt gacgagcagc
     2401 gattcgtaaa cgggatcggg aagcagctcg cgtctgggag ccggaccttt acgaggcatg
     2461 gcctaacccc ccgccttctt cgcgccgtac ttgctgcgac tctgtacccg gtcgcgcacg
     2521 ccggccgtgt cgagggcggc tcggatgatc ttgtagcgaa cgcccggaag gtctttcact
     2581 cggcccccac gcacgagcac gatcgagtgc tcctggaggt tgtgtccgat accggggatg
     2641 taggcggtga tttcgatgcc agaggtgaga cgtactcgag cgaccttgcg aagagcggag
     2701 ttcggcttct tgggtgtggt cgtgtagaca cgggtgcata ccccgcgcct ctgcggcgac
     2761 cccttgaggg ccggcgactt cgacttctcc ctcttgctcc tgcgaccctg gcgaaccagt
     2821 tgctggatcg tcggcaacct tcactccttc tcttcccgcc gaacgtcgag acgttcggcg
     2881 ctcgtgcgcg acccgaagta ggccgctcgt ctcacctcta tgcagcgcaa acgaaggcgc
     2941 tttgtcggtc tggccgagag ataggtggag gcggtgtgcc ccgtgtcctc ccggcgactc
     3001 tggcgtccgg cgatccgtcc caaacctccg cggttcccgg ggcaaccggg cctaccgagg
     3061 ctcaccgaac tcgggtacta ctccccactg cgcccacctc gagcggagga cctccgcccc
     3121 gggcgcgtag agcgcccccg taaggggcgc agcgagcaag gataaggccg cctgaaacct
     3181 tggtcaaacg ctcccgccga gcgtccagac gctcggcgct cgggctaggt gaaaaaggcc
     3241 cccgaaacgg gggccttttt gctgatattt ccttgctcta cgccggggac tcgtcctcgg
     3301 tgtccggagt ggcctcttca ccctccggtt cctggggggt ctcggcctcg gccaccggca
     3361 gatcggtcac cacagcttcg ggggcctcgt cggccttctc tgcctcatcg gcctcctcgg
     3421 ccccgccggt ctcgccccct ccatcttgag ggagcgcatc ctcggctccg cccaggtcgc
     3481 cgaaggactc ttcggctccg cgggcctcac cgaacgccga c
//
LOCUS       DATMED010000365         5811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2989577, whole genome shotgun sequence.
ACCESSION   DATMED010000365 DATMED010000000
VERSION     DATMED010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989577"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1534
                     /gene="ccrA"
                     /locus_tag="VNP73_09515"
     CDS             188..1534
                     /gene="ccrA"
                     /locus_tag="VNP73_09515"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HWL66197.1"
                     /translation="MAGDINAIRDAILNDASGEDIAALELPESTTAAFVRKDEQEMFS
                     GMDSQDKDPRKSLHIDEVPLPPLGPNECLIAVMASAINFNTVWTSIFEPLPTFKFLER
                     FAKTGELGARHDLDYHIVGSDASAVVLRTGPGVTRWKPGDRVTVHCNYVDLEAPEGHD
                     DSMMDPGQKIWGFETNFGGLSEISMVKANQLMPMPSHLTWEEAACNGLVNSTSYRMIV
                     SRNGAQIKQGDVVLIWGATGGLGGYAVQYCLNGGAIPVGVVSSPDKVELLHAIGCEHV
                     IDRKAEGYQFWDGDKQNQKEFVRFGKKIRELVGEDPDIVFEHPGRQTFGASVFVTKRG
                     GKIVTCASTSGFKHEYDNRYLWMMLKDIIGSHFANYKEAWEANRLIDLGMIHPILSKT
                     YPLTETAEAAYQVHNNLHKGKLGILVLSPEEGLGVRDTDKRARFADRIDLFKRFSD"
     gene            complement(1539..2219)
                     /locus_tag="VNP73_09520"
     CDS             complement(1539..2219)
                     /locus_tag="VNP73_09520"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HWL66198.1"
                     /translation="MLDRSVVKAAGGLLMFGAVLGLVANLMHPQLSDPSPREELELVA
                     DSGIWLIDHFLIAWTGAFLLVGLVAIGWSYAEPRAAAWGRFAIASGIVGVGILFLTAL
                     VDGMAVKDAADAWAANPDDPAIYGRALAVMDMQRILFTGIQGSIFGLTPVLFGVAGLM
                     TDEHPKWLAWLVLVAGVVGLLSGSIQYLAGVSRLTADVLFPIASIAFTAWAFIMGLRL
                     WKGSPAQA"
     gene            complement(2248..3444)
                     /locus_tag="VNP73_09525"
     CDS             complement(2248..3444)
                     /locus_tag="VNP73_09525"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWL66199.1"
                     /translation="MAPTVIASAARTAIGKFNGQVSHLPAVDFGASAIKAALERAGIT
                     GDQVDYVIMGQVLQAGTGQITARQASVKAGVPTSVPAITINKVCLSGLNAIAMADQMI
                     RAGEASVVVAGGMESMDQAPYLLPKARTGYRMGDGKLVDSMIFDGLWDAFSHRHMGDQ
                     SDDVNTEFKISRADQDKWAARSHERAQKAIEEGRFKEEIVPIEVPQRKGDPIVFDTDE
                     GVRPGTTVETLAKLKPAFQPEGTITAGNASQVSNGGAAVVVMSAERAQELGVTPIAEI
                     LAHGMSAEEPPYLHTVPALAIQSALKKAGMSPNDIDLIEVNEAFASVAMHAIGMLYNG
                     NKEAEEKVNVNGGAVALGHPIGCTGARITVTLLHELKRRGGGIGAAALCGGGGQGDAI
                     IYRVAG"
     gene            3538..4314
                     /locus_tag="VNP73_09530"
     CDS             3538..4314
                     /locus_tag="VNP73_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWL66200.1"
                     /translation="MTVDIEVRGPVTVVTINRPEVRNAVDGPTAEALTGAFREFERND
                     EQLVAILTGAEGTFCAGADLKAMANDRDRANRVDPEGDGPMGPSRMTLDKPVIAAIEG
                     YAVAGGLELALWCDLRVAGDEATLGFYDRRWGVPLIDGGTIRIARLIGQSRALDMVLT
                     GRGVGAPEAHEWGLVNRLTPSGEALKGALELAEDIARFPHQVMRADRRSLLRQWGLTL
                     EDAMRQEFEWGLEAGAGGETEAGAARFASGKGRHGNFEDI"
     gene            complement(4414..5247)
                     /locus_tag="VNP73_09535"
     CDS             complement(4414..5247)
                     /locus_tag="VNP73_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66201.1"
                     /translation="MEKRLSIASAILMVFAAALAMLLMATPVLASQAEDNGNGKDHKS
                     ESTDEGTDHDGDADSDPDTVEDESEADESTADDDDNAHPSGKDRSIENSPDSNPNQGK
                     AESNPDDTNGPRRAEGVEGEDDKPNAGGGEDSADQDGNNGCGNDDDFDDDNNGWCGKP
                     SRDDDSKDDDDSKDDDDDRDRGKGNDDDDDDDAEVAAAGIFEAPARPLPEEPQVLGSV
                     LSRPARPAVSPGVVPSVVRNARVRGGVLPFTGSELNAFVLFAIGMIASGGVALVATRR
                     N"
     gene            5412..>5811
                     /gene="mce"
                     /locus_tag="VNP73_09540"
     CDS             5412..>5811
                     /gene="mce"
                     /locus_tag="VNP73_09540"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWL66202.1"
                     /translation="MLGKIEHIAVAVADLEAAVAHYEDVWGLKVSHRERVEDQGVEEA
                     MLPLGESHLQLLGATGPDTTVGKFLERRGPGLHHIAYEVADLAATLDELKAKGVPLID
                     EAPRRGGRGHMVAFVHPKGNHGLLVELIQRP"
BASE COUNT         1081 a         1886 c         1810 g         1034 t
ORIGIN      
        1 ccgaagcccc gctcggtgca gcgcgagccc ctcttcttcg ggcatgaacc cgatcgcatc
       61 gcgagcgacg gcgagcaatc gttcatccat cgcccgcaac gtacctgccg taggctccac
      121 tgtctgctaa cggccggttc aacacccctt gggctcggcc accggcgaaa actaatagga
      181 gggctcagtg gccggagaca tcaacgccat cagggacgcg atcctgaacg acgcatcagg
      241 cgaagacatc gcagcgttgg agcttcccga gagcacgacg gcggcgttcg tccgcaagga
      301 cgagcaggag atgttctcgg ggatggactc ccaagacaag gatccccgca aatccctgca
      361 catcgacgaa gtaccgctcc ccccgctcgg ccccaacgaa tgtctgatcg cagtcatggc
      421 ttcggccatc aacttcaaca ccgtgtggac ctcgatcttc gagccgcttc ccacgttcaa
      481 gttcctcgag cgcttcgcca agacgggtga gctcggcgct cgtcacgacc tcgactatca
      541 catcgtcggt tcggatgcct cggccgtcgt gttgcgaacc ggccccggag tgacgcgctg
      601 gaagcccggc gatcgcgtaa cggtgcactg caactacgtg gatctcgagg cacccgaagg
      661 acacgacgac tcgatgatgg acccgggtca gaagatctgg gggttcgaaa cgaacttcgg
      721 cggtctgtcc gagatctcga tggtgaaggc gaatcagttg atgccgatgc cgtcgcatct
      781 cacctgggaa gaagcggcgt gcaatgggct cgtcaactcc acttcgtacc ggatgatcgt
      841 gtcgcgcaac ggtgcgcaga taaagcaggg cgacgtggtg ttgatctggg gtgctaccgg
      901 gggtctcggc ggatacgccg tccagtactg cttgaacggg ggggccatcc cagtcggcgt
      961 cgtgtcgtca cccgacaagg tcgagctgct gcatgccatc ggctgcgagc atgtgatcga
     1021 ccgcaaagcc gagggctacc aattctggga cggtgacaag cagaaccaga aggagttcgt
     1081 ccgcttcggc aagaagatca gagaactcgt cggcgaggac ccggacatcg tcttcgagca
     1141 ccccggccgc cagacgttcg gcgccagcgt gttcgtcacg aaacgcggcg ggaagatcgt
     1201 gacctgtgcg tcgacctcgg gcttcaagca cgagtacgac aaccggtacc tatggatgat
     1261 gctcaaagac atcatcggct cccacttcgc caattacaaa gaggcgtggg aagcgaaccg
     1321 gctgatcgac ctggggatga tccatccgat cctgagcaag acgtatccgc tcaccgaaac
     1381 agccgaggcc gcgtaccagg ttcacaacaa ccttcacaaa ggcaagttgg ggatcctcgt
     1441 tctgtccccg gaagagggtc tgggtgtgcg cgacaccgac aagcgagcgc gcttcgccga
     1501 ccggatcgac ctcttcaaac gcttcagcga ctaagtagct aggcctgggc cgggcttcct
     1561 ttccacaagc ggaggcccat gatgaacgcc catgcggtga atgcgatcga agcgatcggg
     1621 aacagcacgt cggccgtcag acgcgagacg ccggcgaggt actggatcga cccgctcagc
     1681 aggcccacga cccctgccac gaggaccaac cacgcgagcc actttggatg ttcgtccgtc
     1741 atcaaaccgg ccacaccgaa gagaacgggc gtcagcccga atatggagcc ctggataccc
     1801 gtgaagagga tgcgctgcat gtccatcacg gccagggcgc ggccgtagat cgcgggatca
     1861 tccggattcg cggcccaagc gtcggcggcg tccttcacag ccatgccgtc gaccagtgcc
     1921 gtcaggaaga ggatcccgac tccgacgatc cccgacgcga tcgcgaatcg cccccaggcc
     1981 gctgcacgcg gttctgcata cgaccagccg atcgcgacca gcccgacgag caggaaagct
     2041 cctgtccacg cgatcaggaa gtgatctatc agccagatac cgctatccgc tacgagctcg
     2101 agttcctcgc gcggagatgg atcggagagc tgggggtgca tgagattcgc aaccagcccg
     2161 agaacggctc cgaacatcaa gagcccaccg gccgccttaa cgacactcct atcgagcatt
     2221 gcttccccct tggctcggtg cgttagatca accagctacg cgatagatga tcgcgtcgcc
     2281 ctggccccca cccccacaga gtgccgcagc accgatgccg ccgccgcgcc gcttcagctc
     2341 gtgcagcaga gtgacggtga tccgcgcacc ggtgcaaccg atcgggtggc caagagcaac
     2401 ggctccgccg ttcacgttga ccttctcctc ggcttccttg ttcccgttgt agagcatgcc
     2461 gatcgcgtgc atcgcgacgg aagcgaacgc ctcgttaacc tcgatcagat cgatgtcgtt
     2521 cggcgacatc cccgccttct tgagcgcgga ttggatcgcg agagccggaa ccgtgtggag
     2581 gtaaggaggc tcctctgcgg acatgccgtg agccaggatc tctgcgatcg gcgtaactcc
     2641 gagctcctgt gcccgctctg cactcatcac gacgacggca gccccgccgt tgctgacctg
     2701 cgaggcattg ccggccgtga tcgtcccctc gggctggaat gccggcttga gcttggcgag
     2761 ggtttcaacg gtcgtaccgg gacgaacccc ttcgtcggtg tcgaacacga tcggatcgcc
     2821 cttgcgctgc ggaacctcta tgggaacgat ctcttccttg aagcgaccct cttcgatcgc
     2881 cttctgcgcg cgctcgtgcg agcgggcggc ccacttgtcc tggtccgccc gcgagatctt
     2941 gaactcggtg ttcacgtcgt cggactggtc gcccatgtgc cgatggctga acgcgtccca
     3001 caaaccgtcg aagatcatcg agtcgacgag cttcccgtcc cccatccgat agccggtgcg
     3061 cgctttgggc agaaggtaag gagcttgatc catcgactcc atcccaccgg ctacgacgac
     3121 cgaggcctcg cccgcccgga tcatctggtc cgccatcgcg atcgcgttga ggccggagag
     3181 acaaaccttg ttgatcgtga tggcggggac gctcgtcgga acaccggcct tgaccgaagc
     3241 ctgtcgggcg gtgatctggc ccgttcccgc ttggagcacc tgtcccatga tcacgtaatc
     3301 gacctggtcg cccgtgatgc cggcacgctc gagtgctgcc ttgatcgcgg atgcaccgaa
     3361 gtcgactgcg ggcagatgtg agacctgacc gttgaacttg cctatcgcgg tccgagctgc
     3421 tgaagcgata accgtcggag ccatgggtta cccctttcaa aggttgacga acttcgattc
     3481 tacgaactga taccgaccct gcctcccggg cctgggtgca gctaacctag ccgcacaatg
     3541 accgtcgaca tcgaggtacg cggccccgta acggtcgtca cgatcaaccg tcccgaggtg
     3601 cgcaacgcgg tcgacggccc caccgccgaa gcgctgaccg gcgcgttccg agagttcgag
     3661 aggaacgacg agcaactggt ggcgatcctc accggagccg agggaacgtt ctgcgcgggg
     3721 gccgacctca aagccatggc caacgatcgg gaccgagcga accgggtcga cccagaaggt
     3781 gacggcccga tgggtccgtc gcgcatgacc ctcgacaagc ccgtgatcgc agcgatcgag
     3841 gggtacgcgg tcgcgggtgg actcgagctc gcgttgtggt gcgatctgag ggtcgccggt
     3901 gacgaagcga ccctcggttt ctacgaccgc cgttgggggg ttccgctaat cgacggagga
     3961 acgatcagga tcgcgcgcct catcgggcag agcagagctc tcgacatggt cttaaccggg
     4021 cgaggtgtcg gcgcgcccga ggcgcacgag tggggcctcg ttaaccgtct caccccgagc
     4081 ggtgaagcgc tgaagggagc ccttgaactg gccgaagaca tcgctcgctt cccgcaccag
     4141 gtcatgcgcg ccgaccggcg gtcgctgctg cgccagtggg gtctcactct cgaggatgcg
     4201 atgagacagg aattcgaatg gggcctcgaa gcgggggccg gaggcgaaac cgaagcgggg
     4261 gccgcgcgct tcgcctcggg caaaggccgg cacggcaact tcgaagatat ctgagcaacg
     4321 agaagaggta gacatgagaa agccgacggc ctcgaccgtc ggcagaacaa gagagggggc
     4381 ccgaaggccc cctctcttgt attgcattga gccctagtta cggcgggtcg ctacgagagc
     4441 gactccaccc gaggcgatca tgccgatcgc gaacaacacg aacgcgttca gctcactgcc
     4501 ggtgaacgga agaacgccgc cgcgcacccg agcgttgcga acgacactcg gtacgacgcc
     4561 aggagagacg gccggacgtg ccggccgaga cagaaccgaa ccgagaacct gcggctcctc
     4621 ggggaggggc cgggccggcg cttcgaagat tcccgccgcg gcgacttcgg catcgtcgtc
     4681 gtcgtcatcg tcgttgcctt tgccacggtc gcggtcgtcg tcgtcgtcct tcgagtcatc
     4741 gtcgtccttc gagtcatcgt cacgggaagg cttgccgcac cacccgttgt tgtcgtcgtc
     4801 gaagtcgtcg tcgttgccgc aaccgttgtt gccatcctgg tcggcggagt cttcgccgcc
     4861 accggcgttg ggcttgtcat cttcaccctc gacaccctcg gcgcgacgag gtccgttcgt
     4921 gtcgtcgggg ttcgactcgg ctttgccctg gttcgggttc gagtcggggc tgttctcgat
     4981 cgaccggtct ttgcccgacg ggtgagcgtt gtcgtcgtca tccgcggtcg actcgtcggc
     5041 ctcggattcg tcttcgaccg tgtcgggatc cgaatcggcg tcgccgtcgt gatcggttcc
     5101 ctcgtcggtg gactcggact tgtggtcctt accgttgccg ttgtcctccg cctgactcgc
     5161 gaggaccggt gtggccatca gcaacatcgc cagtgccgcg gcgaacacca tcaggatcgc
     5221 cgaagcgatc gacagtctct tctccatgtc tgttctccct ctgtttgccc ggcttggagt
     5281 cactctccgc agccaattgc ttacgttccg ttataggtac tgttcgtggt catatgacta
     5341 cttcctgcat tcgcagggac ttttttccaa actttttccc gcctaaaccc gcctcgtagg
     5401 atgcgggcct catgctgggc aagatcgagc acatcgccgt cgccgtagcc gacctcgagg
     5461 cggccgtcgc ccactacgag gacgtctggg gcctgaaggt gtcccaccgg gaacgggtgg
     5521 aggatcaggg cgtcgaggag gccatgctcc ccctcggcga gtcccacctg cagctcctcg
     5581 gagctaccgg ccccgacacc accgtgggga agttcctcga gcggcggggg ccggggctgc
     5641 accacatcgc atacgaggtc gccgacctcg ccgcgaccct ggacgagctc aaagccaagg
     5701 gggttccgct gatcgatgaa gccccgcggc gggggggccg gggacacatg gtcgccttcg
     5761 tccatcccaa ggggaaccac ggcctgctcg tcgagttgat ccagcgcccc t
//
LOCUS       DATMED010000366         3373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2235851, whole genome shotgun sequence.
ACCESSION   DATMED010000366 DATMED010000000
VERSION     DATMED010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235851"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VNP73_09545"
     CDS             <1..571
                     /locus_tag="VNP73_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HWL66203.1"
                     /translation="AFILFMIAGIAETNRAPFDLPEAETELIAGFHTEYSGIKFALFF
                     LGEYIHIVVVSAMAVTLFFGGWNGPGFDFLPWIWPVFWFLIKTIVFLYIFVWLRATLP
                     RLRYDRLMWLGWKRLIPAALLWIMVTAVVNTEGVSRQVRLAVFAALFLGVLLVVAKGD
                     PRLKQIVTPQRPSRSPRRSKDQEVSSGSA"
     gene            585..968
                     /gene="nuoI"
                     /locus_tag="VNP73_09550"
                     /pseudo
     CDS             585..968
                     /gene="nuoI"
                     /locus_tag="VNP73_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
     gene            1046..1630
                     /locus_tag="VNP73_09555"
     CDS             1046..1630
                     /locus_tag="VNP73_09555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWL66204.1"
                     /translation="MSLDLIVFGAMFVLTLGSAIAMIFARNAVHVALFLVVTQVGLAV
                     AFLLEGAFFVAVVQIIIYAGAIMVLFLFVLMLLGVDRREALHEPLMFQRPAAIGLGAL
                     LTAQVLYVAVRGVDLTFSAGGQEALEEINSDPGNVLGIARVLFGKYLLPFEVTSVLLI
                     AAVVGVMVLARRIYAIAPTTGSQTPEGSEKEVAA"
     gene            1627..1935
                     /gene="nuoK"
                     /locus_tag="VNP73_09560"
     CDS             1627..1935
                     /gene="nuoK"
                     /locus_tag="VNP73_09560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWL66205.1"
                     /translation="MTDVSPGYFLALAGLLFTVGAVGALVRRNAIVIFMFVELMLNAV
                     NLFFVTFARMQGNLDGQVLVFFVLVVAAAEVVVGLALILMIARRRATLDVDNVNLLKW
                     "
     gene            1940..>3373
                     /gene="nuoL"
                     /locus_tag="VNP73_09565"
     CDS             1940..>3373
                     /gene="nuoL"
                     /locus_tag="VNP73_09565"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWL66206.1"
                     /translation="MTLAATAPGGFPLLAAAVIALPLLGSATLLFFGRRLGRLSGFVA
                     TGAIGLSFICGVALFFGLLGEAGEEREFVIKLFDWITVADFQANADLLVDPLSVVMVL
                     IVTGVGTLIHIYSIGYMEGDAGYSRFFAHLNLFCGAMLILVLADNLVLLFVGWEGVGL
                     CSYLLVGFWFERNAAANAAKKAFIVNRIGDFSFLLGIFLIAGEVGSLSVAEVNAGAAG
                     MGATLATIGALLLFGGATGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYVVA
                     RFSPLFEAGGATALTVVAWIGALTALWAALMAAFEYDIKRVLAYSTISQLGYMFLANG
                     VEGYSIGIFHFVTHAFFKALMFLGAGAVMHALAGETDMRKMGGLRKAMPIVGWTFLAG
                     WLAISGIFPFAGFFSKDAILAVTWDHGEYALWGIGLFTAALTAFYMSRMYFRVFEGEL
                     RVPEGVHVHDAPKLMAAALIPLGVLSVV"
BASE COUNT          516 a         1006 c         1088 g          763 t
ORIGIN      
        1 cgcgttcatc ctgttcatga tcgccggaat cgccgagacc aaccgggctc cgttcgacct
       61 tcccgaggct gagaccgagc tgatcgccgg tttccacacg gagtactccg ggatcaagtt
      121 cgcgctgttc ttcctcggcg agtacatcca catcgtggtc gtgtcggcga tggcggtgac
      181 gctgttcttc ggtggatgga acggccccgg ctttgacttc ctgccctgga tctggccggt
      241 gttctggttc ctgatcaaga cgatcgtgtt cctttacatc ttcgtgtggc ttcgagccac
      301 cctgccgagg ctgcgctacg accggctgat gtggctgggg tggaaacggc ttatcccggc
      361 cgccttgttg tggatcatgg tcacggcggt cgtcaacacc gagggcgtgt cgcgccaggt
      421 tcgcctagcg gtcttcgccg cgttatttct cggggtgctg ttggtggtcg caaagggtga
      481 tccgaggctg aaacagatcg tcacccccca gaggccgagc cggtcaccgc ggcgctcgaa
      541 ggaccaggag gtgagcagtg gctctgcctg agatcctcac cggcatgaag gtcgtcatcc
      601 gcaacgtctt caaagccaag gacacggttt cctatccgga ggtacgccgg cagccgttcc
      661 ctcgcttcaa gggtcgtcac atcctcgatc gtcatcccga tggactcgag aagtgcatcg
      721 gatgtgagct gtgcgcgggg gcctgtcccg cggatgcaat ctacgtggtg ggggccgaga
      781 accggccgga cgctcgcttc tcgcccggcg agcggtacgc gcagatctat cagatcaact
      841 atctccgctg catcttctgt gggctgtgcg tggaagcgtg tccgaccgag gcgctgttga
      901 tgacgaccga gtacgagatc tcagggccct cgagggacga gctcatctac accaaggatc
      961 agctcatcat cgacgcaccc atcaagcggc actggaagac caaggtggag tacagccccg
     1021 accgagccac taaggaaagg accaaatgag tctcgacctg atcgtgttcg gggcgatgtt
     1081 cgtcctgacg ctcggctcgg ccatcgcgat gatctttgcg cgcaacgccg tgcacgtggc
     1141 tctgttcctc gtggtcaccc aggttggttt ggccgttgcg ttcctgctcg agggcgcctt
     1201 tttcgtcgcg gttgttcaga tcatcatcta tgcgggagcc atcatggtgt tgttcctgtt
     1261 cgtcctcatg ttgctgggag tcgaccggcg cgaggcgctg cacgaacccc tgatgtttca
     1321 gcggcccgcc gcgatcgggc tgggggcgct gctcacggct caggtgctgt acgtggccgt
     1381 ccgcggggtg gacctcacct tttcggcggg cggacaggaa gcgctcgagg agatcaactc
     1441 ggacccgggc aatgtccttg ggatcgcgcg cgtgttgttc ggaaagtatc tgctgccgtt
     1501 cgaggtgaca tccgtactgc tcatagcggc tgtggtggga gtcatggtgc tcgcccgccg
     1561 catctatgcg atcgccccga ccaccggctc gcaaacaccc gaaggaagcg agaaggaggt
     1621 ggccgcgtga cggatgtctc tcccggttac ttcctggcgc tggctggttt gctgtttacc
     1681 gttggcgccg tcggcgccct cgtgcgacgc aacgcgatag tgatcttcat gttcgttgag
     1741 ctcatgctta acgccgtgaa cctgttcttc gtgacgttcg cgcggatgca gggcaacctc
     1801 gacggacagg tgctcgtgtt cttcgtgcta gtagtcgcag ccgcagaggt cgtcgtcgga
     1861 ctggccctga tcctcatgat cgcgcgccgg cgagcgactc tcgatgtcga caacgtaaac
     1921 ctgctgaagt ggtgacggca tgacgcttgc agccaccgct ccgggcggct tcccactgct
     1981 ggccgctgcc gtgatcgcgc tgcccctcct cggctccgcc accttgttgt tcttcggccg
     2041 gcggttaggt cggctgtcgg gattcgtcgc caccggtgcc atcggcctga gcttcatctg
     2101 cggcgttgca ctcttcttcg gccttctcgg cgaagcaggc gaagagcgtg agttcgtcat
     2161 caagctgttc gactggatca ccgtcgcaga tttccaggcg aacgcggatc tgctcgtcga
     2221 tccactgagt gttgtgatgg tcctgatcgt aacgggggtt gggacgctca tccacatcta
     2281 ttcgatcggc tacatggagg gcgacgccgg ttactcacgc ttcttcgctc acctgaacct
     2341 gttctgcggg gcaatgctga tactcgttct cgccgacaac ctggtgttgc tgttcgtcgg
     2401 ttgggaggga gtcggactct gttcctatct cctggtgggc ttctggttcg agcgaaacgc
     2461 ggccgcaaac gccgccaaga aggccttcat cgtcaaccgc atcggcgact tctcgttcct
     2521 gctcggcatc ttcttgatcg cgggtgaggt cggaagcctg agtgtggccg aggtcaacgc
     2581 aggtgctgca ggcatgggag cgacgctcgc taccatcggg gcattgttgc tgttcggcgg
     2641 cgccacaggg aagagcgctc agattccgtt gtacgtctgg ctccccgacg ccatggaggg
     2701 accgactccc gtctccgccc tgatccacgc ggcgacgatg gtgacggccg gtgtctacgt
     2761 ggtcgctcgt ttcagcccac tgttcgaagc ggggggtgcg accgcgctca cggtcgtggc
     2821 ctggatcggc gcgctcaccg cgttgtgggc cgcgctcatg gctgcgtttg aatacgacat
     2881 caagagggtt ctggcctatt cgacgatcag ccagctcggc tacatgttcc tggcgaacgg
     2941 cgtcgagggt tactcgatcg gcatctttca cttcgtgacc cacgcgttct tcaaggccct
     3001 tatgttcctg ggagcgggtg ccgtgatgca tgcgcttgcc ggtgagacgg acatgcggaa
     3061 gatggggggc ctgcgtaagg cgatgcccat cgtgggctgg acctttcttg ccggctggct
     3121 cgcgatctct gggatcttcc cattcgcggg cttcttctcg aaggacgcga ttctggccgt
     3181 aacgtgggac cacggcgagt atgccctgtg gggaattggg ctgtttacgg cggctctgac
     3241 ggcgttctac atgtcgcgga tgtacttcag ggtgttcgag ggtgagttga gggtgcccga
     3301 aggcgttcac gtgcacgatg cccccaagct aatggctgcg gcgttgatac cgctcggcgt
     3361 gctctcggtc gtc
//
LOCUS       DATMED010000367         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4514133, whole genome shotgun sequence.
ACCESSION   DATMED010000367 DATMED010000000
VERSION     DATMED010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4514133"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VNP73_09570"
     CDS             complement(<1..606)
                     /locus_tag="VNP73_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006289077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWL66207.1"
                     /translation="MAPTKFSFTPGFEMAGDQPNAVEGLVEALEAGDRFTTLLGATGT
                     GKTATMAAAIERLGRPALVMAPNKTLGAQLAAEFREFFPDNAVEYFVSYYDYYQPEAY
                     VPSSDTYIEKDSSINDEIDRLRHAATAAVLTRKDAIVVASVSCIYGLGSPEVYEEQML
                     YLHKGGEIPRDHIVRRLVNLSYQRNDSAVTRGRFRVRGDTLE"
     gene            complement(668..1450)
                     /locus_tag="VNP73_09575"
     CDS             complement(668..1450)
                     /locus_tag="VNP73_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HWL66208.1"
                     /translation="MEAQAHRMIGALRRRGIHPLVLVTIATAWCLAIGVQYGGIAQVI
                     HHDSLIEGGLPLAAALALFLLAWQAMIAAMMLPSSLPLIVLFERTSAAVPDRGQVRAA
                     FLGGYTAVWSLFGVAAFLSDAGLHRLVDASPWLAAHPNVIAGTTLTLAGAFQFSSLKE
                     ACLRACRNPGVYLMNRYRRGARAAFRLGWGHGIFCLGCCWALMLVAFAMGMANLWWMV
                     GFTALMVFEKVGRHGDRAVAPIGIGLISAGALMILGDGLPSF"
     gene            complement(1426..2064)
                     /locus_tag="VNP73_09580"
     CDS             complement(1426..2064)
                     /locus_tag="VNP73_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HWL66209.1"
                     /translation="MGAQNTPTGTGYELDGSLLEACSCGILCPCWVGEDPDLGSCYAF
                     NAYHVDRGVIAGIDVSGLNYIRICDIPGNVLVPGSWKEVVFIDEDATEEQARVLRAAF
                     AGEFGGPLADVAGLIGETVAIERAKIDYQLVEGAGTVRIPGVLDSEMEPYRGADERVT
                     TLRDSLFSTVPGSPAWVGKATAHKVTLPQYGMEWEYEGRNAIQAEWKLKHTG"
     gene            2216..2380
                     /locus_tag="VNP73_09585"
     CDS             2216..2380
                     /locus_tag="VNP73_09585"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HWL66210.1"
                     /translation="MKMAWAYTCECGFVVRGGSDDEFVANAQQHAKSDHNVDLTRQQA
                     LAIAEPAQDG"
     gene            complement(2377..>2775)
                     /locus_tag="VNP73_09590"
     CDS             complement(2377..>2775)
                     /locus_tag="VNP73_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWL66211.1"
                     /translation="TYGLFPDAVPTLDELTTRGMRLGLISNFESWLKDLLDHLQITDR
                     FEVIAVSGDLGWEKPDPRIFKWALEEMGVLPEQSLYIGDSPHFDPGPAIAVGMHGVLL
                     DRHGRWAEFDGDYPRVATLGELPGLIETLG"
BASE COUNT          561 a          894 c          849 g          471 t
ORIGIN      
        1 ctcgagcgtg tcgccgcgca cgcggaaccg gccgcgcgta acggccgagt cgttgcgctg
       61 gtacgagagg ttgacgagcc gccgcacgat gtggtcgcgc gggatctcac ctcccttgtg
      121 caggtacagc atttgctcct cgtagacctc aggagagcca agaccgtaga tgcacgagac
      181 ggaagccacg acgatggcgt ccttccgcgt gaggacggct gctgtagcgg cgtggcgcag
      241 acggtcgatc tcgtcgttga tcgaggagtc cttctcgatg taggtgtcac tcgacgggac
      301 gtaggcctcc ggctggtaat agtcgtagta gctgacgaag tactcgacgg cgttgtctgg
      361 gaagaactcc cgaaactcgg cggccagttg ggcccccagc gttttgttgg gcgccatcac
      421 gagagccgga cgccccagcc tttcgatcgc ggcggccatc gttgcggtct taccggtgcc
      481 ggtagcgccg agcaaagtag tgaaccggtc gccggcttcg agggcttcga cgagaccttc
      541 aacggcgttc ggttggtccc cggccatctc gaagccgggc gtgaacgaga actttgtggg
      601 agccattcag gcaagcctac cggcgttccc gacaactcct tgtcgggaac gctgaagaag
      661 atgcgcttca gaacgacggc aggccgtcgc ccaagatcat cagggcaccg gcagagatga
      721 gaccgatgcc gatcggtgca acggcacggt caccgtgccg gccgaccttc tcgaacacca
      781 tgagagccgt gaatcccacc atccaccaca ggttcgccat tcccatggcg aacgcgacga
      841 gcatcagcgc ccagcagcag ccgaggcaga agatcccgtg tccccagccg agtcgaaagg
      901 ccgccctcgc gccgcggcga tagcgattca ttagataaac gccgggattg cggcacgctc
      961 gcaaacacgc ttccttcaac gacgagaact ggaaggctcc ggccagggtc aatgtcgttc
     1021 ccgcaatgac gttggggtgg gccgccagcc acggcgaagc gtcgacgagt cgatgcaggc
     1081 cggcatcgga caggaaagca gccacaccga acagcgacca caccgccgta tagccgccga
     1141 ggaacgcggc ccggacctgc ccgcggtccg gcactgccgc gctcgtccgc tcgaacagga
     1201 cgatgagagg gagggacgac ggaagcatca ttgcggcgat catcgcctgc cacgcgagta
     1261 agaaaagcgc gagggcggcc gccagaggca gaccgccctc gatcagcgaa tcgtgatgga
     1321 tcacttgcgc gatcccgccg tattgcacgc cgatcgccag acaccaggcc gtcgctatgg
     1381 tcacgaggac caacggatgg atcccgcggc ggcgcagtgc gccgatcatc cggtgtgctt
     1441 gagcttccat tcggcttgga tcgcgtttcg gccttcgtac tcccactcca tgccgtactg
     1501 cggcagcgtt accttgtggg ccgtcgcctt acccacccag gccggcgagc cgggaacggt
     1561 cgagaagagc gagtcgcgga gagtcgtcac acgctcatca gctccgcgat acggctccat
     1621 ctcactatcg agaactccgg ggatcctgac ggttccggcg ccctcgacta gctgatagtc
     1681 gatctttgct cgttcgatgg cgaccgtctc tccgattagc ccggctacat cggccagtgg
     1741 gccgccgaac tcacccgcga acgcggcccg caataccctt gcctgttctt cggtcgcatc
     1801 ttcgtcgatg aagacgactt ccttccacga cccaggaact agcacgtttc ccgggatgtc
     1861 acagatgcgt atgtaattca gcccgctcac atcgataccg gcgatcacgc cccggtcaac
     1921 gtgataagcg ttgaacgcgt agcacgagcc gaggtccgga tcctctccca cccagcacgg
     1981 gcagaggatc ccgcaagaac atgcctccag cagagatccg tcgagctcgt acccggtacc
     2041 cgttggcgtg ttctgtgcac ccacgatctc ctcctctcaa tcgacccctg ggacgtctca
     2101 cgtcgagacg cccgacgatc atgggccgag gatggcggca aagcacgcgg ccgtctgtca
     2161 ggtgtgcgac agccgtgctc tagggttcaa gctcctattg gagacagaaa ggaggatgaa
     2221 gatggcctgg gcatacacat gtgaatgcgg cttcgtcgtt cgaggaggga gcgacgacga
     2281 gttcgtcgcc aacgcgcaac aacatgcgaa gagcgaccac aacgtggacc tcacgcggca
     2341 gcaagcgttg gcgatagccg agcccgcgca ggacggctag ccgagggttt cgatcagtcc
     2401 cggtagctcg cccagcgtgg cgacgcgcgg ataatcgcca tcgaactccg cccacctgcc
     2461 gtgccggtcg agaagcacgc cgtgcatgcc aacagcgatt gcgggcccgg gatcgaagtg
     2521 cgggctgtcc ccgatgtaga gactttgctc gggaagaaca cccatctcct cgagcgccca
     2581 tttgaagatt ctcgggtctg gtttctccca cccaagatca ccggagaccg caatcacctc
     2641 gaagcgatcg gttatctgaa gatggtcaag gagatccttg agccaactct cgaagttcga
     2701 aatcagaccg agcctcatcc cccgcgtcgt caactcgtcc agagtgggaa ccgcgtctgg
     2761 gaacaacccg tacgt
//
LOCUS       DATMED010000368         2983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_309534, whole genome shotgun sequence.
ACCESSION   DATMED010000368 DATMED010000000
VERSION     DATMED010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309534"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VNP73_09595"
     CDS             <1..720
                     /locus_tag="VNP73_09595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL66212.1"
                     /translation="VTCTETNQRHLSRIADATPVHLVYHGLNDDFWRFSQRPIPSRTA
                     NGSLRLLAVGRLVAKKGFDVFVEACGVLDQQNVSFEARIVGEDDDVGDNFSVGEDLRR
                     RITRLGLEERVQLLGQIGQAELYKHYRDASLFCLPCRVLDNGDRDGIPNVLLEAMSCG
                     VPVLSTPVSGIPELIQSGHNGLLVPEDDPAALARALLMLHHDRDLARRLGCAGRASVS
                     ERFDGNLLARQLAQLFEQVMA"
     gene            765..2402
                     /locus_tag="VNP73_09600"
     CDS             765..2402
                     /locus_tag="VNP73_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HWL66213.1"
                     /translation="MPRNVYCITRDVHNDRSVAEAVCTGRFTHAGIVCDLGIEPDWLG
                     ADLPFDEEWRIEWNKFYYGLDLANAFLQTSDTRFLHTWESLVRSWIDQVPIGYDSCDV
                     AARRMQNWTYAWSLFGSSPSFPGLRHGLAGDILQSVDGHARYVKRWLTPQVFRNHRTF
                     ELYSLFIAALAFPSIDPGAELLSFTISELRRTLAEGFRSDGVHRENSTHYHLVALRSL
                     LGARENARRFSLELPGDFDFLLERACEFALHCHRPDGVISALSDADGGSYPEVLELAA
                     SLLDRPDFEYAATSGARGSPPELKHASFPDAGYHFQRSGWGTDGAPFSDERFLVLDCG
                     PLGDGGHGHYDLLSVEVYAHGRPLIVDPGRYTYSEEGANLRRWFKGTAAHNTVCVDGL
                     DQTQYRRGRPGKLTATGRSLGRRNEPGLDLLEAQAKSPSYDAVHTRRVLFVGNEFWIV
                     DDELSAADEHHYDLRWHLSPEAWGATELLTIDRRPVVRAPGLALVFASNLSVEIEPGW
                     VSPSYGSKHPAPVVSASTTGRNARFTTLIVPVRGDRP"
     gene            2399..>2983
                     /locus_tag="VNP73_09605"
     CDS             2399..>2983
                     /locus_tag="VNP73_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66214.1"
                     /translation="MSGVSPMALAPDPAVPQRNHLTDLDAIARRLSAQLDPNGRVPIQ
                     TCRRACVSYRVGKRLRVVYQVGIDGGDLYVTASTFRSSSRSERAYSKAVTNAPYLGGL
                     RPALHDAELDTVFWVFPNDRKIENLSAVADPTDELVHLVDGPWVKSQLVDYYPEVSAV
                     VRCLDDSNRVIAYAKIHVGDEGERTFRVQQELERI"
BASE COUNT          538 a          971 c          910 g          564 t
ORIGIN      
        1 gtcacctgta ccgaaacgaa ccagcgccac ctgagccgaa tcgccgacgc gacgccggtc
       61 catctcgttt accacggtct gaacgacgac ttctggagat tctcccaacg ccctatcccg
      121 agccgcaccg cgaacgggag cctccgactg ctggccgtag gaagactggt tgccaagaag
      181 ggtttcgatg tctttgttga ggcatgcgga gtcctcgatc agcaaaacgt gtcgttcgag
      241 gcaaggatcg tcggggagga cgacgacgtc ggtgacaact tcagcgtcgg cgaggatctc
      301 cggaggcgca tcacccggct ggggctcgag gagcgcgtcc agctgctcgg tcagatcggg
      361 caggcagagc tgtacaagca ctatcgggac gcaagcctct tctgtctccc ctgccgcgtg
      421 ctggacaacg gggaccgcga cgggatcccc aacgtcctcc tcgaggcgat gtcgtgtggt
      481 gtcccggtgc tcagcacgcc ggtgtccgga atccctgaac tgatccagag cggccacaac
      541 ggtctgctgg ttcccgagga cgatcccgcg gctctggcac gcgctctatt gatgcttcat
      601 cacgatcgcg atctggccag acggctgggc tgcgcgggac gagcatcggt gagcgagaga
      661 ttcgacggaa atctgctcgc acgacagctt gcgcaattgt tcgagcaggt gatggcgtga
      721 gcaggggggc ctcgtcggca ccggttgtct ctcaggatcg gattgtgcct cgcaacgtct
      781 actgcatcac gcgggacgtc cacaatgacc ggtcggtcgc ggaagccgtt tgcacgggcc
      841 gcttcacgca tgcgggaatc gtctgcgatc tcggcatcga acccgactgg cttggcgccg
      901 accttccctt cgacgaggaa tggcgaatcg agtggaacaa gttctattac ggactggatc
      961 tggcgaacgc atttcttcag acgagcgaca cgcgcttctt gcacacctgg gaatcactcg
     1021 ttcgctcgtg gatcgaccag gtccctattg gttacgacag ctgcgacgtg gccgcacgca
     1081 ggatgcagaa ctggacctac gcgtggagcc tgttcggctc ctccccgagc tttcccggtc
     1141 tccggcatgg attggccgga gacatcttgc agagcgtcga cggacatgcc cgctacgtga
     1201 agcgatggct caccccccag gtattcagga accaccgcac cttcgagctc tactcgctct
     1261 tcatcgccgc tctcgccttc ccctcgatcg accccggcgc ggagctcctg agctttacca
     1321 tctcggagct gcgccggacg ctcgcagagg ggtttcgatc cgacggcgtt catcgcgaga
     1381 actcgactca ctatcatctc gtggctctcc gatcgctgtt gggcgcccgc gagaacgccc
     1441 ggcgcttctc gctcgaactc cccggcgact tcgacttcct ccttgaacgg gcatgcgagt
     1501 tcgccctcca ttgccatagg ccggatggcg tcatctccgc actgtccgat gcggacggag
     1561 gttcctatcc cgaggtgttg gagctcgctg cttcgctgct cgatcgaccc gacttcgagt
     1621 atgcggcgac ctcgggagcg cggggtagcc cgcccgaact gaagcatgcc tccttccccg
     1681 acgccggtta ccactttcag cggagcggct ggggcactga tggagcccct ttctcggacg
     1741 aacgtttcct cgtcttggac tgcggtcccc tgggggacgg cggacacggt cattacgatc
     1801 tactgagcgt cgaagtctac gcgcatggcc gcccgctgat cgtggaccca ggccggtaca
     1861 cctactccga agagggagcc aacctccgcc gctggttcaa gggcactgca gcgcacaaca
     1921 cggtctgcgt cgacggactc gatcaaacgc agtaccgcag aggaaggccc ggcaagctga
     1981 ccgcaacggg gcgctcgctt ggtcgtagaa acgagccggg actagacctg ctggaggccc
     2041 aggcgaaaag ccccagctat gacgcggttc acactcggcg cgtcctgttc gtcggcaacg
     2101 agttctggat cgtcgacgac gaactgagcg ccgccgacga gcaccattac gacctgcgct
     2161 ggcacctctc gccggaggcc tggggcgcaa ccgaactgct aacgatcgac cggcgtcccg
     2221 tcgtgcgagc tcctggactt gcgctcgtgt tcgcctcgaa cttgtccgtc gaaatcgagc
     2281 ctgggtgggt ctcgccgagc tacggttcga aacacccggc tcccgtcgtg agcgcgtcga
     2341 ccactggccg caatgcgagg ttcaccacgc tgatcgttcc cgtacgcggg gatagaccgt
     2401 gagcggcgtc tctccgatgg cgctcgcacc ggatcccgcc gttcctcaac ggaatcacct
     2461 gacggatctc gatgcgatcg cgcgacgcct ttccgcacaa ctcgatccca acggtcgcgt
     2521 cccaatccaa acttgccgcc gggcgtgtgt cagttatcgc gtcggcaaac gccttcgtgt
     2581 ggtgtaccag gtcggcatcg acggcgggga tctgtacgtc actgcaagca cctttcgcag
     2641 cagtagccgg agtgagcgcg cgtactccaa ggcggtgact aacgcgccct acctcggagg
     2701 gctccgcccg gccttgcacg acgcggaact cgacaccgtg ttctgggtat ttccgaacga
     2761 tcgcaagata gagaatctgt ccgccgtcgc ggaccccacc gacgaactgg ttcacctcgt
     2821 tgatgggcct tgggtcaaga gccaactcgt cgactactac ccagaggtct ctgcggtcgt
     2881 gcgttgcttg gacgactcga accgcgtcat cgcctacgca aagatccacg tgggagacga
     2941 aggagaacgc acgtttcggg tgcagcaaga gttagagcgt atc
//
LOCUS       DATMED010000369         3180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_309735, whole genome shotgun sequence.
ACCESSION   DATMED010000369 DATMED010000000
VERSION     DATMED010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309735"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VNP73_09610"
     CDS             complement(<1..1456)
                     /locus_tag="VNP73_09610"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWL66215.1"
                     /translation="MKAVLNKVRPTRPPQPAGINDLLKQVRSKRPKANLKLIERAYEM
                     AEEAHRGQRRRSGDPFITHPIAVAAILAELGMDEATIAAALLHDAVEDTELSLAEVEP
                     EMGFDVTALIDGVTKLDKIGFRTAEHTRAENLRKMIVATAKDIRVLLIKIADRLHNMR
                     TLGPLNPDRRELIARETLEIYSPLAHRLGMYAIKWELEDLAFATLYPKRFEEIETLVE
                     DRQPEREEALEEVAEQIQAKLREVKIKAEISGRPKHLYSIYEKIMLGGKGLDEIFDLV
                     GIRLIVDNVKDCYATLGALHTLYTPVPGRFKDYIAMPKFNMYQSLHTTVMVPGGRPME
                     IQIRTRDMHRAAEFGIAAHWLYKEQRKGKVKEEEMAWLQRMMDWQQESMDPKDFMESL
                     KIDLYADEVFVFTPKGDVISLPLNATPIDFAYSIHTEVGQRTVGSRVNGKLVALNHEL
                     HSGDSVEIITARGAAAPSRDWLQVVKTARARNKIR"
     gene            complement(1558..1839)
                     /locus_tag="VNP73_09615"
     CDS             complement(1558..1839)
                     /locus_tag="VNP73_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66216.1"
                     /translation="MPGTQERRSLKYIRLAAFLASQPAEVDQLEMKLEEIEDVVGEKL
                     PNGARFPAWWRNDSRRMHSRAWLTAGWRVEGFNGDGIVRFERQANDGAN"
     gene            complement(1904..2428)
                     /locus_tag="VNP73_09620"
     CDS             complement(1904..2428)
                     /locus_tag="VNP73_09620"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWL66217.1"
                     /translation="MSEHDWLKGLIRDIPDFPKEGVVFKDITPLLADQKAFTYTIDAL
                     FHHYDRDEIDKVLGIEARGFIVAAPLAYRFTAGLIPVRKQGKLPWKLEHVTYELEYGT
                     DRLEVHKDAIETGERILIVDDVLATGGTARATAQLTERCGGRVAGIATIMELGFLKGR
                     DKVAEYDFFSLLSY"
     gene            complement(2509..>3180)
                     /gene="secF"
                     /locus_tag="VNP73_09625"
     CDS             complement(2509..>3180)
                     /gene="secF"
                     /locus_tag="VNP73_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWL66218.1"
                     /translation="SKAIRALLIFLVVVSLFISLRFEWKMALGALIALLHDLIVTAGA
                     YAAVGFEVTPSTIIAILTILGYSLYDNVVVFDKLEENTVAYAATGKMTYQDSANLAVN
                     QVFMRSLNTSLTTMLPVGALLFVGAGLLGATTLKDLALALLVGMLVGAYSSVFVAVPI
                     LTMLKEREPRYKNVREKVLRDAKRASLQPATAGAQADGGQTAGSSSQPRARAGSKKAK
                     RRKRR"
BASE COUNT          534 a         1013 c          978 g          655 t
ORIGIN      
        1 gacggatctt gttgcgagcg cgggccgtct ttacgacctg cagccagtcg cgcgacggcg
       61 ccgcggcgcc gcgcgcagtg atgatctcga cggaatcgcc ggagtgcagt tcgtggttga
      121 gcgcgaccaa cttgccgtta acccgagatc caaccgtgcg ttggccgacc tcggtgtgga
      181 tcgaatatgc gaagtcgatc ggcgtggcgt tcaacggcag ggagatcacg tcgcccttgg
      241 gagtgaagac gaaaacctcg tccgcgtaga gatcgatctt cagcgactcc atgaaatcct
      301 tcggatccat ggactcctgc tgccagtcca tcatcctctg gagccacgcc atctcttcct
      361 ctttgacctt gcctttgcgt tgctctttgt agagccagtg cgcggcgatc ccgaactccg
      421 ccgcccggtg catgtctcgc gttctgatct ggatctccat cgggcggccg cccggcacca
      481 tcacagtggt gtgaagggac tggtacatgt tgaacttggg catcgcgatg tagtccttga
      541 accgcccggg aaccggcgtg tagagggtgt ggagcgcacc cagcgtcgcg taacaatcct
      601 tgacgttgtc cacgatcaac ctgatgccga ccagatcgaa gatctcgtcg agccccttgc
      661 cgcccaacat gatcttctcg tagatcgaat acaggtgttt ggggcgaccc gagatctcgg
      721 ccttgatctt gacctcgcgc agcttggcct ggatctgctc ggccacttct tcgagtgctt
      781 cctcccgctc cggctggcgg tcctccacca gagtctcgat ctcctcgaag cgcttcgggt
      841 aaagcgttgc gaaggcgagg tcttccaact cccacttgat cgcatacatg ccgagcctgt
      901 gcgccagcgg cgaatagatc tcgagggtct cgcgagcgat cagctcgcgg cgatccggat
      961 tcagcggccc gagcgtgcgc atgttgtgca ggcggtcggc gatctttatc agtaggacgc
     1021 ggatgtcctt cgccgttgcg acgatcatct tgcggaggtt ctcggccctc gtgtgctcgg
     1081 ccgtcctgaa gccgatcttg tccagcttgg tcacgccgtc gataagtgcc gtcacgtcga
     1141 atcccatctc gggctcgacc tcggcgagcg atagctccgt gtcctcgacc gcgtcgtgca
     1201 gcagagcggc ggcgatggtg gcctcgtcca tccccagctc ggccaggatc gcggcaaccg
     1261 cgatcgggtg cgtgatgaag gggtctccag agcgccgccg ttggccccgg tgggcctcct
     1321 ccgccatctc gtaggcacgc tcgatcagct tcagattggc cttgggtcgc ttggacctca
     1381 cctgcttcag caggtcgttg atcccggcgg gttggggggg ccgggtggga cgaaccttgt
     1441 tgaggacggc tttcatccca cgagccgacg ggccgcccgg ctcgggtccc ttagggggcg
     1501 acttgggaga agacgcgctc tccggcatgg agcccatagt tcattctatg ggtgggatca
     1561 gttcgctccg tcgtttgctt gacgttcgaa gcggacgatg ccgtcgccgt tgaagccttc
     1621 gacccgccac ccggccgtca gccacgcgcg gctgtgcatc ctgcgagagt cgttccgcca
     1681 ccacgccggg aaccgagccc cattgggcag tttctccccc acgacgtctt cgatctcttc
     1741 gagcttcatc tcgagctggt ccacttcggc gggttgagac gcgaggaagg cagccaggcg
     1801 tatgtacttg agggaacgtc gctcctgcgt cccaggcatg gcctcacagt gaaggctctt
     1861 atcgttggta gatagggggt ttcctaaatg cccgaaatct tcctcagtac gacaggaggc
     1921 tgaagaagtc gtactcggcc accttgtccc ggcctttcag gaagccgagc tccatgatcg
     1981 tcgcgatccc ggccacccgt cccccacacc gctcggtgag ctgcgccgtc gcccgagccg
     2041 tccctcctgt tgcgagcaca tcgtccacga tcaggatccg ctcgcccgtc tcgatcgcgt
     2101 ccttatggac ctcgaggcga tcggttccgt actcgagctc gtaggtcacg tgctcgagtt
     2161 tccacgggag cttcccctgc ttcctaaccg ggatcagacc tgcggtgaac cggtacgcga
     2221 gtggagcggc cacgatgaaa ccgcgcgctt cgatccccaa gaccttgtcg atctcgtccc
     2281 ggtcgtagtg atggaagagc gcgtcgatcg tgtaggtgaa ggccttctgg tccgcgagga
     2341 gcggggtgat gtccttgaac acgacgccct ctttcgggaa gtcggggatg tcccggatca
     2401 gtcccttgag ccagtcgtgt tcgctcatat gtcctccctc agatatgccc gctgcgcggg
     2461 gcagctcttc gcttcggacg gcgcgatcgt cgagacgatc gctgggacct agcgccgttt
     2521 tctgcgtttg gccttcttgc tgccggcgcg ggcgcgcggc tgggacgacg agcccgcggt
     2581 ttggcctcca tccgcctgag cacccgcggt cgcgggctgc agcgaggctc gcttcgcatc
     2641 gcgcaagacc ttctccctga cattcttgta cctcggttcg cgttccttga gcatcgtcag
     2701 gatcggcacc gcgacgaaca cggacgagta cgcgccgacg agcatcccca cgagcagagc
     2761 gagggccagg tccttcagcg tcgtcgcgcc cagtagaccg gcccccacga acagcagcgc
     2821 gcccaccggc agcatggtcg tcaacgaagt gttgagcgac cgcatgaaga cctggttgac
     2881 ggccaagttg gccgagtctt gataggtcat cttgcctgtg gcggcatacg caaccgtgtt
     2941 ctcttcgagt ttgtcgaaga cgacgacgtt gtcgtacagc gaataaccga ggatcgtgag
     3001 gatcgcgatg atcgtcgaag gagtcacctc gaaacccacc gccgcgtaag cccctgcggt
     3061 gacgatcagg tcgtggagca gcgctatcaa cgctccgaga gccatcttcc attcgaagcg
     3121 caacgagatg aacagtgaca ccaccacgag gaagatcagg agcgcgcgga tcgccttgga
//
LOCUS       DATMED010000370         4843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5287385, whole genome shotgun sequence.
ACCESSION   DATMED010000370 DATMED010000000
VERSION     DATMED010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5287385"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1796
                     /locus_tag="VNP73_09630"
     CDS             222..1796
                     /locus_tag="VNP73_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66219.1"
                     /translation="MRKLIADRQSVAILLVALMLTAACGSTVQAPQATQLGDDELGDL
                     STGGALPPGATINDKGQVVSSSGEVLGSAEDFGVSVPDGANGGAGPVAGGTNGGTGGT
                     VGGSGGGAALGPGVTSDKIYLGIVYLDAGAANQALAGQAIESDARKAYDAMIEEVNKA
                     GGLGGREVVPIYHKYDADSSETIAQQAQAACARFTQDNKVYAILAFDNGAGVIQECVE
                     RAGAVQPVHYNGGSLPEDFRKYPHYVEITGMNALRVGPVTVTGLKREGYFDKGFKLGI
                     VTWDDAGYKKSVDEGYIPALAKQGIELSTEPAYISPPQTYDDLAAMSADINNAILRFQ
                     SQGITHVIIAAGPSGLCGFACMETVWFRRAESQNYRPRYGLNDNNLPTGAYDQGLYPA
                     EQLRDAVAVGWADLNKASDEGTRVNQARESCFDLMRKKGVPMGNVNQENDARQACDEL
                     RFLQLIVNVKLDGVPRVNDQFIAAVNTLGTSFPSTAAYGTHFSAAQHDGIAAARNLKF
                     LASCDCWRYTTDPYRV"
     gene            1834..3366
                     /locus_tag="VNP73_09635"
     CDS             1834..3366
                     /locus_tag="VNP73_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66220.1"
                     /translation="MVLVLIAAACGTTASPQQQQAAETATDDLGSLDSALPPGATIND
                     KGQVVSSSGEVLGSAEDFGISTGSGRSVGGPVADGGTGSSSGGSGLRSGGGQGVPAAA
                     APGITDSKIYVGVGWSDAGAANQAFAGAALNSDIRDPMNAMIEEVNKSGGIAGRKIEP
                     IYHNYEAASSQTIDQQDQAACARYTQDNKVFAILGDGDIMQQCAEKAGAVLLTVTSTS
                     LPEDFQRYPHFIEISGMNLIRVGNVSVTGLHREGYFSDGAKLGLVTWDEPNYRDAVER
                     GYLPALKKKGVKPATDTAYISSPQTADDLAAASADVNNAVLRFQSQGITHVMLIDGAT
                     PLCRGGCLGILWTRRSESQNYRPRYGFNAGNAAKAAQEQGLYPTEQLGGSIAVEWGDS
                     DKFADQGWKLNKARERCYDIMRKRNVPMDNANKEADARTACEFIWFLQTVIDVELGNA
                     TITNDNFIAGVNALGWTYSSAMAYAVHFSATQHDGNSAARNMRFVDNCECYRWTGDPY
                     RV"
     gene            complement(3354..3752)
                     /locus_tag="VNP73_09640"
     CDS             complement(3354..3752)
                     /locus_tag="VNP73_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66221.1"
                     /translation="MTQPALPNDPVTSEERKWLDRALVVIRFVRDPAFPGVALLATVI
                     LAGFLALVVTWSGVARTIYVALQIPVIVSGGLGALALIGLGAALLTVQLDRREAAREQ
                     RLMDGWIDEIVELMGHVPALRARRTRGQTR"
     gene            complement(3752..4180)
                     /locus_tag="VNP73_09645"
     CDS             complement(3752..4180)
                     /locus_tag="VNP73_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66222.1"
                     /translation="MTTESQQIQSDPQDAPTGTISAIDPPPSSGSATPAPRRKRLARL
                     RQFVGAGAGVWVGLFLVAGGFGLIAYTWGEVAGLLDVALQLPFLVSGGLTALGLILLG
                     LLVVNIAVKRKDALERQRQLEEVREALVRLREAIESQDED"
     gene            complement(4188..>4843)
                     /locus_tag="VNP73_09650"
     CDS             complement(4188..>4843)
                     /locus_tag="VNP73_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL66223.1"
                     /translation="DVTILGPEARAHRGLIRSFQDSALFPTMTVTDAVAVALERALPT
                     SFFSSVVGLSFRERSKQQQARELVSLMGLNPYRDMQIMELSTGTRRITEIACLVATRP
                     TLLLFDEPSTGIAQRETEALGDLLRHIKDTLGVTLLVIEHDMPLIMGLADRIICMADG
                     KIIASGPPDVVQQNPLVVEAYLGGSLSAIERSGPVRTQDDLVPAGGPTPGGSTLEGK"
BASE COUNT         1014 a         1541 c         1504 g          784 t
ORIGIN      
        1 accacccccc ggttcgccca tcgagtgtga cttcgccccc aggggccccc gtccctgccg
       61 gctaaccaag cgatcgcccg ggagctaggc cgcaggctgg tggcgacccc caaccgggga
      121 tagggcgatt ggtcggccgc gacagcagga aggggcccgc tgagggtgca tatttccctt
      181 aagggatctt ctggcagaca ccgcgttcta aggggggcgt gatgcggaag ctgatcgcgg
      241 atcgacagag cgtcgcgatc cttctcgtcg ccctcatgct gactgccgcc tgcggctcga
      301 ccgttcaggc accccaggca acccaactcg gcgacgacga gctgggcgac ctttcgaccg
      361 gcggcgccct gcctccggga gccaccatca acgacaaggg ccaggtcgtc agttcgtccg
      421 gagaagtatt gggatcggcc gaggacttcg gcgtttcggt gcccgacggt gccaacggcg
      481 gggccggccc ggtggccgga ggcacgaacg gtggaacggg cggaaccgtc ggcggttccg
      541 ggggcggggc cgcgctggga cccggtgtca cctcggacaa gatctatctc ggcatcgtct
      601 atctcgacgc gggggcggcg aaccaggccc tcgccggtca ggccatcgag tccgacgccc
      661 gcaaggctta cgacgcgatg atcgaagagg tcaacaaggc gggcggtctc ggtggccgcg
      721 aagtcgtgcc gatctatcac aagtacgacg cagactcgtc ggagacgatc gcccaacaag
      781 cacaggctgc ttgtgcacga ttcactcaag acaacaaggt ctacgcgatc ctggccttcg
      841 acaatggagc cggcgtcata caagaatgcg tggagagagc cggagccgta caacccgtcc
      901 attacaacgg cggctcgctg cccgaggact tccgcaagta tccccactac gtcgagataa
      961 ccgggatgaa tgctctacgg gtgggccccg taacagttac gggtctgaag agagagggct
     1021 acttcgacaa gggtttcaag ctcgggatcg tcacttggga cgacgccggc tacaagaagt
     1081 cagtcgacga ggggtacata ccggcactcg ccaaacaagg catcgaactc tccacggagc
     1141 cggcctacat cagcccgccg cagacctatg acgaccttgc ggcgatgagc gcagacatca
     1201 acaacgcgat cttgagattc cagtcgcagg ggataacgca cgtgatcatc gccgcgggcc
     1261 catcgggctt gtgcggattc gcatgcatgg agacggtgtg gttccggagg gccgagtcac
     1321 agaactaccg ccctcgttac ggattgaacg acaacaacct gccgaccggg gcgtacgacc
     1381 aggggctcta cccggccgag caactccggg atgcagtcgc ggttgggtgg gccgacctga
     1441 acaaggcgtc cgacgaggga acgcgggtca accaggcgcg ggagagttgc ttcgacctca
     1501 tgcggaagaa gggcgtcccg atgggcaacg tcaatcagga gaacgacgcg cgccaggcgt
     1561 gcgacgagtt gcggttcctt cagttgatcg tcaacgtcaa gctggatgga gttccaaggg
     1621 tcaacgacca gttcatcgcc gcggtcaaca ccctcggcac gtcgttccct tccacggccg
     1681 catacggaac ccatttcagc gcggcgcaac acgatgggat cgccgccgcc cgcaacctca
     1741 aattccttgc atcgtgcgac tgctggcgtt acaccaccga cccgtaccgg gtctaggagg
     1801 aactacgtga aaaagtcgag cctttggatc ctgatggttt tggtcttgat cgccgcggcg
     1861 tgcggaacga cagcttcgcc gcaacagcag caagcagcag aaaccgcgac cgacgatctc
     1921 ggttcgctcg acagcgcgct tcctccggga gcaacgatca acgacaaggg tcaggtcgtc
     1981 agctcctccg gcgaagttct gggctcggcc gaggacttcg gcatcagcac cgggtccgga
     2041 agatcggttg gaggcccggt agctgatggt ggcaccggtt cgagttccgg tggaagcggg
     2101 ttgaggagtg gtggaggtca aggcgtaccg gcggccgcgg cccccgggat cacggactcc
     2161 aagatctacg tcggggtcgg atggagcgat gcgggggccg ccaaccaagc cttcgccgga
     2221 gcagccctca actcggacat ccgcgacccc atgaacgcca tgatcgaaga ggtcaacaag
     2281 agcggcggga tcgccgggcg caagatcgag ccgatctacc acaactatga ggcggcctca
     2341 tcccaaacga tcgatcaaca agaccaggct gcgtgcgctc gttacaccca agacaacaag
     2401 gtgttcgcga tcctgggcga cggcgacatc atgcagcaat gcgcggagaa ggccggtgcg
     2461 gtcctactga cggtaaccag cacttcgctg cccgaggact tccagagata cccccacttc
     2521 atcgagatct ccgggatgaa cctgatccgg gtggggaacg tatccgtgac cggtcttcac
     2581 cgcgagggct acttcagcga cggcgccaag ctcgggttgg tcacgtggga cgagcccaac
     2641 taccgcgacg ccgttgagag aggctacctg cctgcgctga agaagaaagg cgtcaaaccc
     2701 gctaccgaca ccgcctacat ctcgtcaccc caaaccgccg acgacctcgc cgcggccagc
     2761 gccgatgtca acaacgctgt tttgcggttc cagtcgcaag gcatcaccca cgtcatgctc
     2821 atcgatggcg ccactcccct ctgtagaggg ggatgtctcg ggatcctgtg gacgcgacga
     2881 tccgaatcgc agaactacag gccacgctac gggttcaacg ccggcaacgc ggccaaggcc
     2941 gcccaggaac aggggctgta tccgacagaa caactaggtg gctccatcgc agtggaatgg
     3001 ggcgactctg acaagttcgc agaccaaggc tggaagctaa acaaggccag agagcgctgt
     3061 tacgacatca tgcgcaaacg caacgttccg atggacaacg ccaacaagga agccgatgca
     3121 cgaacggcat gcgagttcat ctggttcttg caaaccgtca tcgacgtcga gctcggcaat
     3181 gccaccatca cgaacgacaa cttcatcgcc ggagtgaacg ctctcggctg gacctattcg
     3241 tctgctatgg cgtacgccgt gcacttcagc gcgacgcaac acgacggcaa ctcggcggcg
     3301 agaaacatgc gattcgtcga taactgcgag tgctaccgct ggacggggga cccctaccgc
     3361 gtctgacctc tggttcgacg cgcacgcagg gccggaacgt gccccatcag ctcaacgatc
     3421 tcgtcgatcc atccgtccat caaacgttgc tctcgcgccg cttcacggcg atccaactga
     3481 accgttagca aagcggcccc caatccgatc agcgccagcg cgcccaaccc tcccgacaca
     3541 atgacgggga tctgaagggc aacgtagatc gtgcgagcca ctccactcca agtaacgacc
     3601 aacgcgagga atccggccag gatcacggtc gccaacaacg cgacgccggg gaaggcagga
     3661 tcgcgaacga acctgatcac cacaagtgca cggtcgagcc acttgcgttc ctcggaagtg
     3721 accggatcgt tcggcaacgc cggctgagtc atcagtcctc gtcctgactc tcgatcgcct
     3781 cgcgcaggcg caccagtgct tcacgcactt cctcgagttg ccgctgtcgc tcgagcgcgt
     3841 ctttgcgctt caccgcgatg ttgaccacca acagcccgag gaggatgagt ccgagagcgg
     3901 tgagcccacc agatacgagg aatgggagct gcagcgcgac gtcaaggagc cctgcgacct
     3961 caccccaggt gtaggcgatc agaccgaaac caccggcaac gaggaagaga ccgacccaca
     4021 cgccggcacc ggcaccgacg aactgcctga gtcgcgcgag ccgtttgcga cgcggtgccg
     4081 gtgtcgcgga tccgctcgag ggcggcggat ctatcgcact gatcgtgccg gtaggagcat
     4141 cttggggatc actttggatc tgctgcgact ctgtggtcat cgctatctca ctttccttcc
     4201 agggtactcc cgcccggtgt cgggccaccg gccggaacca gatcgtcctg cgtgcgcacc
     4261 ggccccgacc gttcgatggc actgagactg ccgccgagat atgcctcgac gacgagtggg
     4321 ttctgctgca cgacatccgg cggccctgaa gcgatgatct tgccgtccgc catgcagatg
     4381 atgcgatccg ccaaccccat gatcagcggc atgtcgtgct cgatcaccaa caacgtgact
     4441 ccaagcgtgt ctttgatgtg gcgaaggaga tcgccgaggg cctcggtctc gcgctgtgcg
     4501 atcccggtcg aaggctcgtc gaaaagcaag agtgtcggcc gagtcgcgac aaggcaggcg
     4561 atctcagtga tccggcgcgt gccggtcgac agctccatga tctgcatatc gcggtagggg
     4621 ttcaggccca tcagtgagac gagctcgcgg gcttgttgtt gcttcgagcg ttcacggaac
     4681 gacaatccca ccaccgatga gaagaagctc gtcggcagtg cgcgttccag agccacggct
     4741 acggcatccg tgaccgtcat cgtcgggaag agcgcagagt cctggaagga tcggatcaga
     4801 ccgcgatgag cgcgcgcttc aggcccaagg atcgtcacgt ctt
//
LOCUS       DATMED010000371         3810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1834183, whole genome shotgun sequence.
ACCESSION   DATMED010000371 DATMED010000000
VERSION     DATMED010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834183"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..309
                     /locus_tag="VNP73_09655"
     CDS             103..309
                     /locus_tag="VNP73_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWL66224.1"
                     /translation="MLNLPQDLAAALQTAALEDGFAALQPDVQSGFTTWLEHAGDDSL
                     RKRRIERIVAAVKEIEKDASPRGS"
     gene            330..1437
                     /gene="prfB"
                     /locus_tag="VNP73_09660"
     CDS             join(330..383,385..1437)
                     /gene="prfB"
                     /locus_tag="VNP73_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156801994.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWL66225.1"
                     /translation="MDDLAERLDAVERTLEHVRSTFDVESKRARVKRLRDSSAAPDFW
                     DDSTSAQKVMAEMSRVAEDVEAYDRMQQRVSDTRVLWELASAESDESTAAEATSELVA
                     IEDDLSSLEVMSLLGGDFDDNPAILEVHAGEGGTDSQDWANMLVRMYQRWAENRGYKA
                     DLVAVTPGEEAGIKGATLTIEGRHAYGLLAADRGVHRLVRISPYDSAKRRHTSFASVD
                     VTPEVEEDVEVEIGPDDLRVDTYRSSGAGGQHVNVTDSAVRITHLPTGIVVSCQNERS
                     QMQNRAVAMRILKSRLLARAIEEREAEMKALSGEKMEIGFGSQIRSYVLHPYQMVKDH
                     RTGMEVGNVQGVLDGDIDKFIEAELRRKAAASTD"
     gene            1456..2526
                     /locus_tag="VNP73_09665"
     CDS             1456..2526
                     /locus_tag="VNP73_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HWL66226.1"
                     /translation="MTETVENTVERVEKASLQDLRSVVIDDVYVQMDYLLQSRATPMD
                     LYHRWEKQNWSTQDLDFTEDAQHWKSMTGVFEAFQIELQRSFTLFFVGEQAVTDTLSP
                     LVHAAPDEPARIFLSTQLVDEARHAVFFSKFFEEVVGISGGLSAALSVLRDRTVGGFR
                     QIFDNQLVEATEAVRRDPHDYAAWVEGITIYHLIVEGMLALTGQKYLLSILRTMRILP
                     GFQAGFTAVARDESRHVNFGVRAIMEAGIREPELLDRVSSAVFRLLEPAVRTVAAPDR
                     KFAIQPEESPEEFRVNPYEVRDFSLHSLIKRLKVAGLSDDTLAEVRERGIAYYDACWE
                     EYEQEHGLEHPRRFYDRLAAAT"
     gene            2612..3298
                     /gene="ftsE"
                     /locus_tag="VNP73_09670"
     CDS             2612..3298
                     /gene="ftsE"
                     /locus_tag="VNP73_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006550350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWL66227.1"
                     /translation="MIKTLDVVKDYKGAVRAVDGITLDVEKGEFVFIVGPSGSGKSTM
                     MKLLTKEEEPTGGEIYVAGKNLATLPRWRVPYLRRNVGCVFQDFKLLPSKTVFENVAF
                     GLEVIGRPRSVVQRQVPQILELVGLGEKLDRFPEELSGGEQQRVSIARAFVNRPLILL
                     ADEPTGNLDPATSVGIMRLLDRINRTGTTVVMATHDHAIVDSMRRRVVELEDGKIVRD
                     QSRGIYGVGS"
     gene            3298..>3810
                     /locus_tag="VNP73_09675"
     CDS             3298..>3810
                     /locus_tag="VNP73_09675"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWL66228.1"
                     /translation="MRVGYFVSETVTNLRRNLLMTIAAISTVAISLLLLGGVQILGIV
                     VRNVTSTIESKVEISTYLRDNATQGEIQELTAQLSQMPEVEDVIYISKEEAFEEFKAT
                     YESQPEIWETLPRDALPASLRVKLLDPDDTEEVAARIEGAPGVSDVRFGGEVIKRLLR
                     VNALLRTIALV"
BASE COUNT          779 a         1115 c         1229 g          687 t
ORIGIN      
        1 gaacggtcgt ccgggtcgaa agggacgatc gagaacctta gggaggaata caagccccgc
       61 ggggcgtatc ttcagtggac ctttcagcgt tgaaagtgag tgatgttgaa cctccctcaa
      121 gatctggccg ccgcattaca gacggcggcg ttggaagacg gcttcgccgc gctccaacca
      181 gacgtacaga gcggcttcac cacctggctc gaacacgccg gcgacgattc cctgcgtaag
      241 cgcaggatcg agcgcatcgt tgcagccgtg aaggagatcg agaaggacgc gagtccacgg
      301 ggctcctagc cctgtagatt cgtcggtcga tggatgatct ggccgagcga ctggacgcag
      361 tagagcggac ccttgagcac gtcaaggagt acctttgacg tcgaatcgaa gcgcgcccgc
      421 gtaaagaggc tgcgcgatag ctcggcggcc cccgacttct gggacgactc cacctcagct
      481 cagaaagtga tggccgagat gtcccgcgtg gcagaagacg tcgaggccta cgaccgcatg
      541 caacaacggg tttccgatac tcgggttctg tgggagttgg cgtcggccga gagtgatgaa
      601 tcgaccgcag ccgaagccac atccgagctg gtcgcgatcg aagacgacct ttcgtccctc
      661 gaggtcatgt cactgctcgg tggggacttc gacgacaatc cggcgatcct cgaggttcac
      721 gccggcgagg gcgggaccga ttcccaggac tgggccaaca tgctggtgcg gatgtaccag
      781 cgctgggcgg agaaccgcgg ctacaaggcc gatctggttg ccgtgacgcc cggcgaggaa
      841 gccggcatca agggtgcaac gctcactatc gaggggcgtc atgcctacgg tctcctcgcg
      901 gccgatcgcg gtgtgcaccg tttggtgagg atctctccat acgattcggc caagcgacgg
      961 cacacgtctt tcgcctcggt cgacgtaacc ccggaagtcg aggaagacgt ggaggtcgag
     1021 atcggcccgg acgacctgcg cgtcgatacc taccgttcct cgggggccgg cggccaacac
     1081 gtcaacgtca ccgattccgc ggttcgcatc acgcacctcc cgacggggat cgtcgtgtcg
     1141 tgccagaacg agcgatccca gatgcagaac cgagcggtgg ccatgcggat cctgaagtct
     1201 cgcctgctcg ctcgcgccat cgaggaacga gaggccgaga tgaaggctct gtcgggggag
     1261 aagatggaga tcggcttcgg gtcgcagatc aggtcatacg tgctgcatcc gtaccagatg
     1321 gtgaaggacc accggacggg tatggaagtc ggaaatgttc agggggtcct ggacggagat
     1381 atcgacaagt tcatcgaggc ggagctcagg cggaaggccg cagcgagcac cgactagcag
     1441 tgggatgatg gggccatgac agagaccgtc gaaaacaccg tcgagagagt cgagaaagct
     1501 tcgctgcagg atcttcggtc cgtcgtgatc gacgacgtgt atgtgcagat ggactatctg
     1561 ttgcagtcgc gggcaacgcc gatggacctg taccaccgct gggagaagca gaactggagc
     1621 actcaggacc tggatttcac cgaggacgcc cagcattgga agtcgatgac cggggtgttc
     1681 gaggccttcc agatcgaact acaacgctcg ttcaccctct ttttcgtcgg cgaacaagcc
     1741 gtcacggaca cgctgtctcc actggtgcac gcggcccccg acgaacctgc tcgcatcttc
     1801 ctatcgaccc agctggtcga cgaagctcga catgcggtct tcttctcgaa gttcttcgaa
     1861 gaggtagtag ggatctcggg cggcttgagc gcagctctca gtgttcttag ggaccgaacg
     1921 gttggcggct tccgccagat cttcgacaac caactggtcg aggccaccga agccgttcgc
     1981 cgggatccgc acgactatgc ggcatgggtc gaggggatca cgatctacca cctgatcgtc
     2041 gaggggatgc tggctctcac cgggcagaag tacctcctgt ccatcctccg aacgatgagg
     2101 atccttcccg gcttccaggc agggttcacc gcggttgcac gcgacgagtc gcggcacgtc
     2161 aacttcggag ttagagcgat catggaagcg ggcatccgcg agccggaact gctcgaccgc
     2221 gtttcttcgg cggtcttccg tctgttggag ccggccgtgc gtacggttgc tgcaccggat
     2281 cgcaagttcg cgatccagcc ggaagaatca cctgaggagt tccgcgtcaa cccctacgag
     2341 gtgagggact tctcgctgca ctcgttgatc aagcgactga aagtggccgg actctcagac
     2401 gacaccctgg cggaggtacg cgagcgcggg atcgcgtatt acgacgcgtg ttgggaagag
     2461 tacgagcagg agcacggcct cgaacacccg cgccggttct acgatcggtt ggctgccgcc
     2521 acttaatttg gcgatacccg gccttaggca agaaacggca gtgaccaggg ggttcgtcgt
     2581 cctggttgga aggacttagc ctggaaacga catgatcaag acactggatg tagtcaagga
     2641 ctacaagggc gcagtgcgcg ccgtcgatgg gatcacgctc gatgtggaga agggcgagtt
     2701 cgtcttcatc gtcggcccct cgggctcggg caagtccacg atgatgaagc tcctcacgaa
     2761 agaggaggag ccgaccggcg gcgagatcta cgtggccggg aagaacctcg ccacgctgcc
     2821 ccgctggagg gtcccgtacc tccgccgcaa cgtcggctgc gtgttccagg acttcaagtt
     2881 gctgcccagc aagacggtgt tcgagaacgt cgcattcggc ctcgaggtga tcggtaggcc
     2941 ccgctcggtc gtgcagaggc aggtccctca gatactcgag ctcgtgggcc tcggcgagaa
     3001 gctcgatcgt ttccccgagg agctgtccgg cggtgagcag cagcgggtct cgatcgcccg
     3061 cgcgttcgta aaccggccgt tgatcctgct tgcggacgag ccgaccggga acctagatcc
     3121 cgcgacctca gtcggcatca tgaggctgtt ggatcgcatc aataggacgg gaacgaccgt
     3181 ggtcatggca acccacgacc acgcgatcgt cgactccatg cgtaggcggg tggtcgaact
     3241 cgaggacgga aagatcgtgc gcgatcaatc tcgagggatc tatggagtcg gtagctgatg
     3301 agggtcggtt acttcgtatc tgaaacggtc acgaacctcc ggcggaacct cctcatgacc
     3361 attgccgcga tctctaccgt agcgatctcg ctgttgctgc tagggggagt acagatcctg
     3421 gggatcgtcg ttcgcaacgt tacgagcacg atcgagtcaa aggtggagat ctccacctat
     3481 ctacgcgaca acgccacaca gggcgagatc caggagctca ccgcgcagct gtcgcagatg
     3541 cctgaggttg aagacgtgat ctacatctcc aaagaggaag ccttcgagga gttcaaggcg
     3601 acttacgaga gccagcccga gatctgggag acccttccca gagacgcact tccggcctcc
     3661 cttcgagtca agctcctcga ccccgacgac acggaagagg tagccgcccg gatcgaaggg
     3721 gcacccggcg tgagtgacgt tcgcttcggc ggcgaagtga tcaaaaggtt gctccgcgtg
     3781 aacgcgctgc ttcgaacgat cgccctcgtg
//
LOCUS       DATMED010000372         3710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3755927, whole genome shotgun sequence.
ACCESSION   DATMED010000372 DATMED010000000
VERSION     DATMED010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755927"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2551)
                     /locus_tag="VNP73_09680"
     CDS             complement(<1..2551)
                     /locus_tag="VNP73_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL66229.1"
                     /translation="MFRKNDVWARPPGGPIEKTAESSFIVRRFPFLKDQPLFPISILF
                     ILNAVDEFDRVAFLILAPEIRDAFGLSNTAFGVIVGLYTVFILIGGLPVGLVGDRFKR
                     TPLVVVAAALWGTMTVFTGLARSLWMLIIARIFAGTGRVANETLHTSLLTDYYPREIQ
                     GRIFAIHRAANPVGNILGPLLAGVLASVADWRWAFFLIAIPTLITATFALRLKEPTRG
                     ETEDKEKADEAAKEAPIPFTRAWRWLFAVPSLKRIYTSAFFGGGAVFAILPYINLFYE
                     EEYAIGAFGRGLIGAATQGGGFMIGALIGGALTDRLRQKSVGRMAFLAGMGTAGIGLG
                     VIMIGISPVLWVAILGGLVAYISIGMWYVPSTSVLAVVLPARVRSLGIGIGVMFFGCG
                     GFVFTVIAGIIADGPGLGTAVTALAPMLWIASALYLNAARFVSDDAQRALDALNTEAE
                     LRKERLEVGSTALIAARGVDVAYDGVQVLFGVDFEIREGEIVALLGTNGAGKSTLLRA
                     ISGTMHPVGGAIFFEGQNITFYEPHETARAGIVQVPGGRGVFPSLTVEENLRVAAWLS
                     TDAATLKEITRETLELFPVLKERWEMRAGDLSGGEQQMLTLALSFIARPKLLMIDELS
                     LGLAPIVVQQLLESVKKIHANGTTVLIVEQSATLALEIAQRAYFMEKGEVRFSGASKD
                     LLGRSDLLRSVFLEGASRGTPVTERFAAKRPARASNGEGEIALEVEGLSKSYGGIAAV
                     DEVAFSVPSGQILGIIGPNGAGKTTVFDLISGFQVPDEGKIVYRGGDVTSLSPHARAR
                     KGLGRSFQHARLFPSLTVAEAIEVAFERNLPAKNPLPAALGLPHVRKAERKL"
     gene            complement(2552..>3710)
                     /locus_tag="VNP73_09685"
     CDS             complement(2552..>3710)
                     /locus_tag="VNP73_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL66230.1"
                     /translation="RGGRSEELEASTWQQLREIRPIPRELKGLPEVRIGFRVATLALL
                     AALVAFALTAPPFRVGQVALILILAMIGLSLVVLTGWAGQISLGQMGFAGVGAAVTGA
                     LLIKQDWNILLALLAAGAIGAVVAAIVGLPALRIRGLFLAASTVAFGLIVGTVVLNQN
                     FFGWLLVRDRIERPPVFGYDLTDDRSFFFFVLAVFALTLYSARSLRKSRLGRVLIAAR
                     DNEAAVKAYGVNVTAARLTAFAFSGFYAAVAGGLFALHQNAVSATTFSVEASLQVFVM
                     VVIGGLGSIPGALLGAAYYFSVLFFLPPIYSLLATGLGMVVLLMIVPGGLGQVAYGWR
                     DGLLRWVAKRNDVVVPSLVADMRTDDSVLLPGVRREAPEAEKEPVPTGGGS"
BASE COUNT          742 a         1240 c         1168 g          560 t
ORIGIN      
        1 ccaacttccg ttcggccttg cgtacgtgcg gaagtccgag tgcggcgggc aacgggttct
       61 tggccgggag gttccgctcg aaggccacct cgatcgcttc ggcaacggtg agggatggga
      121 acaggcgcgc gtgctggaac gaccggccga ggcccttacg ggcacgagcg tgcggcgaca
      181 acgaagtcac gtctcccccg cggtaaacga tcttcccttc gtcgggaacc tggaagccgg
      241 agatcagatc gaacacggtg gtcttgcccg cgccgttcgg tccgatgatc cccaggatct
      301 gaccggaggg caccgagaac gcgacctcgt ccaccgcggc gatgccccca tacgatttgc
      361 tgagcccctc cacctcgaga gcgatctcgc cttcgccgtt actcgctcgc gcaggccgct
      421 tggcggcgaa ccgctccgtg acgggtgtgc ctctcgacgc gccttcaagg aacaccgacc
      481 gcaacagatc gctcctgccc aacaggtcct tggacgcacc cgagaaacga acctctccct
      541 tctccatgaa ataggcgcgc tgggcgatct cgagcgcgag cgttgccgac tgctcgacga
      601 tcaggacggt cgttccgttg gcgtggatct tcttgacgct ctccaagagc tgctggacga
      661 cgatgggggc aaggccgagc gagagctcgt cgatcatcag gagcttcggc cgtgcgatga
      721 acgaaagagc cagtgtgagc atctgctgct cgccaccgga gaggtcaccg gcccgcatct
      781 cccagcgttc tttcaacacg gggaagagct cgagcgtctc gcgcgtgatc tctttcaaag
      841 tggctgcgtc ggtcgagagc caagctgcca cgcgcaggtt ctcctccacg gtgagtgagg
      901 ggaacacccc gcggcccccc ggcacctgca cgatccccgc gcgtgcggtc tcgtgaggtt
      961 cgtagaacgt gatgttctgc ccctcgaaga agatcgcccc tccgacggga tgcatcgtgc
     1021 cggagatcgc cctcagaagg gtcgatttcc ccgctccatt cgtgccgagg agcgcgacga
     1081 tctcgccctc gcggatctcg aagtcgacgc cgaacaagac ctgaaccccg tcgtaagcga
     1141 cgtcgacgcc gcgagccgcg atcagagcgg tggagccgac ctcgaggcgc tctttgcgca
     1201 gctcagcttc tgtgtttagg gcgtcgagtg cgcgctgggc atcgtcactg acgaagcgcg
     1261 ccgcgttgag atagagcgca gacgcgatcc acaacatcgg cgcgagggca gtgacggctg
     1321 tgccgagccc gggcccatcc gcaatgatcc ctgcgatcac ggtgaagacg aagcctccgc
     1381 aaccgaagaa catgactccg atgccgatcc cgaggctccg gaccctggcc ggcagaacga
     1441 cggcaagaac cgaagtgctg ggtacgtacc acatgccgat cgagatatag gcgacgaggc
     1501 caccaaggat cgccacccac aggaccggcg agatcccgat catgatgacg ccgagcccga
     1561 tcccggccgt gcccatcccg gcgaggaaag ccatcctgcc gaccgacttc tggcgcagac
     1621 gatcggtcag cgcacctccg atgagcgctc cgatcatgaa ccctccgcct tgcgttgccg
     1681 cgccgatgag tccccgaccg aaggcgccga tcgcgtactc ctcctcgtag aagaggttga
     1741 tgtaaggaag gatggcgaag acggctccgc cgccgaagaa cgccgatgtg tagatccgct
     1801 tgaggcttgg aaccgcgaac aaccatcgcc acgcacgtgt gaacgggatc ggcgcttcct
     1861 tcgcagcctc gtctgccttc tccttgtctt ccgtctcgcc gcgcgtcggt tccttcagac
     1921 gcagagcgaa cgtcgccgtg atgagtgtcg gtatcgcgat caggaagaac gcccagcgcc
     1981 agtcggcgac cgacgcaagc actccggcga ggagaggccc caggatgttt ccaacgggat
     2041 tcgccgcacg atggattgca aagatgcggc cctggatctc gcgcgggtag tagtcggtca
     2101 gcagactcgt gtgcagcgtt tcgttcgcga cgcgaccggt cccggcgaag atcctggcga
     2161 tgatcaacat ccacaaggac cttgcgaggc cggtgaagac cgtcatcgtt ccccacaggg
     2221 ccgcggcgac gacgaccaga ggtgtccgtt tgaatcgatc gcccaccaat cccaccggaa
     2281 ggccgccgat caggatgaag accgtataga gaccgacgat cacgccgaag gcggtgttcg
     2341 agaggccaaa ggcgtcgcgg atctccgggg cgaggatcag gaacgcgacc cggtcgaact
     2401 cgtcgaccgc gttcaggatg aacaagatcg agatcgggaa gagcggctgg tctttgagga
     2461 atgggaaacg ccggacgatg aacgacgact cagcggtctt ctcgatcggt ccgcccgggg
     2521 gccgcgccca cacgtcgttc ttgcggaaca cctaggagcc ccctccggtg gggacgggct
     2581 ccttctcagc ttcaggcgcc tcgcgccgga ccccaggcaa gagaaccgaa tcgtcggttc
     2641 gcatgtcggc gacgaggctc ggcaccacaa cgtcgttccg cttcgcaacc cagcggagca
     2701 acccgtcccg ccacccgtac gcgacttggc ccagcccgcc gggcacgatc atcaacagca
     2761 cgaccatccc gagaccggtc gcgagcaagc tgtagatcgg cggcaggaag aacagcacgg
     2821 agaagtaata ggccgcgccc agcaaggccc ccggtatcga cccgaggccc ccgatcacca
     2881 ccatcacgaa gacctgcaag ctggcctcga cagagaaggt cgtggccgaa actgcattct
     2941 ggtgaagggc gaaaagcccg ccggcaacgg ccgcgtagaa cccagagaag gcgaaagccg
     3001 ttagccgcgc cgccgtcacg ttgacgccgt acgccttgac cgccgcctcg ttgtcgcgag
     3061 ccgcgatcag caccctgccg agccggctct tccggagcga tcgggccgag tagagggtca
     3121 gcgcgaacac cgccagcacg aagaagaaga acgagcgatc gtcggttaag tcgtacccga
     3181 acaccggcgg ccgctcgatc cggtctcgca cgagtaacca gccgaagaag ttctggttga
     3241 gcactacggt gccgacgatc aagccgaagg caacggtaga ggcggccagg aacaggcccc
     3301 tgatccgtag agcgggtaag ccgacgatcg cagccaccac cgccccgatc gcccccgcgg
     3361 cgagcagggc caagaggatg ttccagtcct gcttgatcag gagagcgccg gtgaccgcgg
     3421 cccccactcc cgcgaacccc atctggccga gggatatctg tcccgcccac ccggtcagta
     3481 cgacaaggga gagcccgatc atggcgagga tcaggatcaa cgccacctgg ccgacacgga
     3541 aaggcggggc ggtgagcgcg aacgcgacca gcgcggccag aagtgccagt gtcgccaccc
     3601 ggaagccgat ccggacctcg ggaaggccct tcagctcgcg cgggatcggg cggatctctc
     3661 gcaactgctg ccacgtcgac gcttcgagct cctccgatct tccgccccgt
//
LOCUS       DATMED010000373         5568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2618857, whole genome shotgun sequence.
ACCESSION   DATMED010000373 DATMED010000000
VERSION     DATMED010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2618857"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VNP73_09690"
     CDS             complement(<1..178)
                     /locus_tag="VNP73_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HWL66231.1"
                     /translation="MAKDSSFDVVSEVDLQEVRNAIDQAQREITQRYDFKNTGSAIEL
                     TDENNNPIVVIKSNT"
     gene            complement(179..529)
                     /locus_tag="VNP73_09695"
     CDS             complement(179..529)
                     /locus_tag="VNP73_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL66232.1"
                     /translation="MADSGATTVLIVDDEEEIRHVLRLLCEAEGFEVVGEAENGVEAI
                     PLALKHQPNVVILDYLLPRLDGSGAAEIMRAITPNSKIVAFSAILEESPVWADAFLNK
                     DRVSELMPLLKSFI"
     gene            755..1279
                     /locus_tag="VNP73_09700"
     CDS             755..1279
                     /locus_tag="VNP73_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWL66233.1"
                     /translation="MLLARARFVPGTRVVITIVMVIGLLFAGTPASSMQVHLANSGGG
                     RYGFKSAEVCFMRKINQARARRGLSSLSWDKQLGYVARKHARQMASNRTVYHDPNMGQ
                     EVTRWRSLAQNTGRGGNCQSTFRHFMRSAPHRYNIMDQWRFTGVGIDYSHGSVYVQMI
                     FETRDNPGNVYHWP"
     gene            complement(1276..2190)
                     /locus_tag="VNP73_09705"
     CDS             complement(1276..2190)
                     /locus_tag="VNP73_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWL66234.1"
                     /translation="MRLRIFGLACLLALSGACIPPEPDDDVIIDWDPEETVMGEIQEK
                     GELVAAIPVDRMPFGDIDDGAGKGLTADLARLIGDALGVETRFVKVEQAELITGPEDQ
                     TVDISFPLVAITERRVRKNIFSDPFYIAHQRLLVQPDSPIQQVEDISGAACQFVAQEV
                     ITEAGRVFVQDIGVDLKEIDPELTVTSPPDPATCLEELDRGRIEAISASDWLLYPAVA
                     ENPDRKIVGDQLSTEGYGAVIESGASAWQDFVNSVFAEADQEGDWQRFYDSWFGPYVD
                     EPVTYPEMTVEEVAALFPKPEVSPAPDS"
     gene            complement(2196..3044)
                     /locus_tag="VNP73_09710"
     CDS             complement(2196..3044)
                     /locus_tag="VNP73_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWL66235.1"
                     /translation="MNVFLAQGGSSVNWFEFLFSPDRWKWLFSGSFFKFLLEGIAVNL
                     EMAVIAMILSLILGLFLALGRISKNPLVSKPSGIWVDVWRNLPLIFILLYLSLALPND
                     VKRAWEEASPDFLPEAFQSGQIVAAIVGLTLYNSAVIAEIMRAGIQSLERGQGEAAAA
                     LGLPYWKSMRLIILPQGLRRMVPATVSQLITLNKDTTLAFIIGFQDVGRHGRILASCQ
                     PFTCGETVPFLQVFLVIGAMFILVNFSLSRLSRRLEIRQRERTGVIVERATGLEDQVA
                     AEADVA"
     gene            complement(3041..3703)
                     /locus_tag="VNP73_09715"
     CDS             complement(3041..3703)
                     /locus_tag="VNP73_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWL66236.1"
                     /translation="MGEFWEVLTGHFPEFAAGFLTTVRLVAVAFVIAMAVGTFVASFR
                     VTPSKWLNRLGGVYVEYFRNVPLLILLFIIFYGLPQELHIEETVAGTVGLGLYTAAYV
                     AETIRSGVFAVGKGQIDAGLSLGFTYAKTLRRIVLPQAVRTVIPPLGSLIIAMIKNSA
                     VIGGALAVSDLLRTAREVNADAGSAFTHETFFWAAAGYLVLTIAATIVVRRLETRFAI
                     HR"
     gene            complement(3776..4696)
                     /locus_tag="VNP73_09720"
     CDS             complement(3776..4696)
                     /locus_tag="VNP73_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWL66237.1"
                     /translation="MYELRRRRWLLAALAAVAMLGAACGGGETTPTDTGGGEEDAMEK
                     FPAGSTMAKLQERGTITVGVKYDVPLFGFKNPQSGEVEGFDVDLAQRVADELGVELEL
                     KEAISDNRIPFLQDGTVDLVFSTMTITTDRDADIDFSRPYYIAHGRILTAGDSGIEDV
                     KDLTADTTVCTALDSTYETTVLPKQAPDAEVKTPDTYSECFAQLQRGSVDALVTDDVI
                     LAGFLQQDESMAIVGPDLTTDPYGAGIKEGDTEFADFVSGVLDETLEDGTWQELFDKW
                     LGQYTGQEAEDPAPWTLEQALETYPCVETC"
     gene            complement(4729..5484)
                     /locus_tag="VNP73_09725"
     CDS             complement(4729..5484)
                     /locus_tag="VNP73_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWL66238.1"
                     /translation="MSAQSSNGHLVEIDHVKKAFGSNVVLDDVSLSIDRGEAVVVAGV
                     SGSGKSTMLRCINGLETVDSGDIRFDGQSIAKAGKSIFGLRAEIGMVFQQFNLFPHKT
                     VLENITLGPVEVKDVSEKEALDRATKLLERVGIPEKADQYPADLSGGQQQRVAIARAL
                     AMEPKLMLFDEPTSALDPEMIREVLDVMRDLAREGMTMVVVTHEMGFAREVCDRIVFI
                     DEGKIVEEGPPEEFFKNAKSQRARDFVDKILHH"
BASE COUNT         1058 a         1723 c         1661 g         1126 t
ORIGIN      
        1 cggtgttgga cttgatcacg acgatgggat tgttgttctc gtcggtcagc tcgatggccg
       61 agccggtgtt cttgaagtcg taacgctgcg ttatctcgcg ctgagcctgg tcgatcgcgt
      121 tacgcacctc ttgcagatcg acttcagaga cgacgtcgaa agatgagtct ttggccattt
      181 agatgaacga tttgagtaga ggcatcagtt cgctaaccct gtccttgttg aggaatgcat
      241 ccgcccacac cggcgattct tcgaggatgg ccgagaatgc aacgatcttc gagttcggtg
      301 tgatggctcg cattatctcc gctgcgccgc tgccgtcgag acgaggaagc aagtagtcga
      361 ggatcacgac gttgggttga tgcttgagcg cgagcgggat cgcttcgacg ccgttttctg
      421 cttcaccgac cacttcgaat ccttcagctt cgcacagcaa acgaagcaca tgccggatct
      481 cttcttcgtc atcgacgata agaaccgtcg tcgcgccgct gtcagccatc acgcaacttt
      541 ctcatgtgag caagagaatc aaaaggacgg attttcgcag tctttcgcga ggaatgtaac
      601 agtctgaaga cgcgcgctca ctctctgtat agacctccga actttgtttg acacatggga
      661 aggaggttgg taccaactac cgaaactttc ccaaaagttg tcggttgaga agcgggctat
      721 ctcccctcgc gggggccagg aaggaggcgc acacgtgttg cttgcgcgcg cgcgattcgt
      781 ccctggaacc agggtcgtca taacgatcgt catggtcatc ggtttgctgt tcgccggaac
      841 gcctgcatct tcgatgcagg ttcacctagc gaatagcggc ggcggaagat acgggttcaa
      901 atccgccgaa gtttgtttca tgcgcaagat caaccaagcg cgcgctcggc gtgggctgtc
      961 gtccctgtcc tgggacaagc aactcggtta cgtcgctcgg aagcatgccc gacagatggc
     1021 ttcgaaccgc accgtgtacc acgacccgaa catgggccaa gaggtgacgc gctggcgttc
     1081 gctcgctcag aacacgggtc gcgggggcaa ctgccagagc acgttcaggc acttcatgcg
     1141 ttcggcgcct caccgctaca acatcatgga ccaatggcgc ttcaccgggg tggggatcga
     1201 ctactcgcac ggttccgttt acgtgcagat gatcttcgag acgagagaca acccgggcaa
     1261 cgtctaccac tggccctagg agtcgggcgc gggtgaaacc tccggcttcg ggaacagcgc
     1321 ggctacttcc tcgaccgtca tttctggata ggtgacgggc tcgtcgacat aggggccgaa
     1381 ccaactgtcg tagaagcgct gccagtcacc ctcctggtcg gcttcggcga acaccgagtt
     1441 cacgaagtcc tgccacgccg acgcaccgct ctcgataacg gcgccgtatc cctccgttga
     1501 gagttggtcc ccgacgatct tgcgatcagg gttttcggcc acggccgggt agagcagcca
     1561 atctgatgcg ctgatcgcct cgatccgacc tctgtccagc tcctcgagac aggtcgcggg
     1621 gtcgggcggc gacgtcaccg tcaattccgg atcgatctct ttcaggtcga ccccgatgtc
     1681 ctgaacgaag accctccccg cttccgtgat gacctcctga gcgacgaact ggcacgccgc
     1741 accggagatg tcctcgacct gttggatcgg ggagtccggt tgcacgagta gccgctggtg
     1801 ggcgatgtag aaggggtccg agaagatgtt cttgcgaacc cgacgctcgg ttatcgccac
     1861 gagcgggaac gagatgtcga cggtctgatc ctccggtccg gttatcaact ccgcctgttc
     1921 gaccttcacg aagcgggtct cgacgcccaa agcgtctccg atcagtcgcg ctagatcggc
     1981 cgtcagacct ttgccggctc catcgtcgat gtccccgaaa ggcatccgat cgacaggtat
     2041 ggctgcaacc agctcaccct tctcctggat ctcgcccatc accgtctcct ccgggtccca
     2101 gtcgatgatc acgtcgtcgt cgggctccgg ggggatgcac gcgcccgaca gggcgagcag
     2161 gcatgcaagg ccgaagatcc tgagtcgcat ggcggtcagg ctacgtcggc ttcggctgca
     2221 acttgatcct cgagcccggt ggcgcgttcg acgatcacgc cggtgcgctc gcgttgtctg
     2281 atctcgagcc ggcgcgacag ccgggagagc gagaagttga cgaggatgaa catcgctccg
     2341 atgacgagga agacttgaag gaatggcacc gtctcaccgc aagtgaacgg ttggcaactc
     2401 gccaggatgc gaccgtgcct gccgacgtcc tggaaaccga tgatgaacgc gagggtggtg
     2461 tctttgttca gcgtgatcag ctgcgacacc gtggccggca ccattcgacg aagcccttgc
     2521 ggcaggatga tcaagcgcat cgatttccaa taggggagtc cgagcgcggc cgccgcctct
     2581 ccctgccccc gctcgaggga ttggatccct gcacgcatga tctccgcgat caccgcagag
     2641 ttgtaaaggg ttaacccaac gatcgcagcg acgatctgac ccgactggaa cgcctcgggg
     2701 aggaagtccg gcgacgcctc ttcccacgcg cgcttcacgt cgttcggaag cgccagtgat
     2761 aggtacagca ggatgaagat caacggcagg ttgcgccaca cgtccaccca aatccccgat
     2821 ggcttgctca cgagcgggtt cttcgagatg cggcccaacg caaggaacag cccgaggatc
     2881 aacgacagga tcatcgcgat cacggccatc tcgaggttca cggctatccc ctcgagtagg
     2941 aacttgaaga agctgccgct gaaaagccat ttccaccgat cgggcgagaa cagaaactcg
     3001 aaccagttga ccgatgagcc cccctgggcg aggaacacgt tcatcggtgg atcgcaaacc
     3061 tggtttcgag gcgacgcacg acgatcgtcg ccgcgatcgt aaggacgagg tagcccgctg
     3121 cggcccagaa gaacgtctcg tgagtgaacg ccgaccccgc gtccgcgttg acctcgcgtg
     3181 ccgtgcgcaa gaggtccgaa accgccagcg ccccgccgat cacggcagag ttcttgatca
     3241 tggcgatgat caggcttccg agtggcggga tgacggtgcg gacggcttgc ggaagcacga
     3301 tgcggcgcaa ggtcttcgca taggtgaatc cgagtgacag gccggcatcg atctgtccct
     3361 tgcctaccgc gaagacgccg gatcggatcg tctcggcgac gtaggcagcc gtgtagagac
     3421 ccaaccccac ggtcccggcg acggtctcct cgatatgcag ttcctgcggg agcccgtaaa
     3481 agatgatgaa gaggaggatc agaagcggga cgttacggaa gtactcgacg tacacaccgc
     3541 cgagccggtt cagccattta ctcggtgtga ctcggaaaga tgcgacgaac gttcccaccg
     3601 ccatcgcgat gacgaacgca accgcgacga gccggacggt ggtgaggaag ccggcggcga
     3661 attccgggaa gtgcccggtc agcacctccc agaattcgcc cacctaactc ctttcaagcc
     3721 gttcttggct aactggctat acctgtgacg tacctgagtt agccaagaac ggttactagc
     3781 aggtttctac gcaggggtag gtctcgaggg cttgctcgag ggtccatggg gccgggtcct
     3841 cggcctcttg cccggtgtac tgcccgagcc acttgtcgaa gagctcctgc caggtcccgt
     3901 cctcgagcgt ctcgtcgagc actccggaga cgaagtcggc gaactcggtg tcgccttcct
     3961 tgatgccggc gccatacggg tccgtcgtca ggtcgggacc cacgatcgcc atgctctcgt
     4021 cttgctgcag gaaaccggca aggatcacgt cgtctgtcac cagcgcatct accgaaccgc
     4081 gctggagctg agcgaagcac tccgagtaag tgtccggtgt cttcacttcg gcgtccggtg
     4141 cctgcttcgg caggacggtc gtctcgtaag tcgaatccag agcggtgcat acggtcgtgt
     4201 ctgccgtcag gtcctttacg tcttcgatgc ccgagtcacc cgctgtcagg atgcgaccgt
     4261 gcgcgatgta gtacgggcgc gagaagtcga tatctgcgtc gcggtcggtc gtgatcgtca
     4321 tcgtcgagaa gaccaggtcg acggttccgt cctggaggaa cgggatccgg ttgtccgaga
     4381 tcgcttcctt gagctcgagc tcgacgccca gctcgtctgc aactcgctgc gcgaggtcga
     4441 cgtcgaatcc ttcgacttca cccgactgcg ggttcttgaa cccgaacagc ggaacgtcgt
     4501 acttgacccc gacggtgatc gtgccgcgtt cctgcagctt cgccatcgtc gaaccggccg
     4561 ggaacttctc catagcgtct tcttcgccac cgcccgtgtc ggtcggcgtc gtttctccgc
     4621 ctccacatgc ggcccccagc atcgcaaccg ctgcgagtgc tgctagtaac caccttctcc
     4681 ttctgagttc gtacatcgaa ctcccccttt cccttctctg ttgtcttgct agtggtgcag
     4741 gatcttgtcc acgaaatcgc gtgcccgctg actcttggcg ttcttgaaga actcttccgg
     4801 aggcccctcc tcgacgatct ttccctcgtc gatgaagacg atccggtcgc acacctccct
     4861 ggcgaacccc atctcgtggg tgactactac catcgtcata ccttctcgtg cgagatcgcg
     4921 catgacgtcg aggacctctc tgatcatctc ggggtcgagg gccgaggtgg gctcgtcgaa
     4981 caacatcagc ttgggttcca tggcgagggc ccgtgcgatc gcaacccgtt gctgctggcc
     5041 gccggacagg tcggccggat actggtcggc cttctcgggg atcccgacgc gctcgagcag
     5101 tttcgtcgcc ctgtccagag cctctttctc ggatacgtcc ttgacctcga ccgggccgag
     5161 agtgatgttc tcgagcacgg tcttgtgcgg gaacaggttg aactgctgga acaccatccc
     5221 gatctcggcc cggaggccga agatcgactt gcccgccttc gctatcgact gcccgtcgaa
     5281 acggatgtcg cccgagtcga ccgtctcgag cccgttgatg catcgaagca tcgtggactt
     5341 gccggacccg gagacccccg cgacgacgac ggcctcgccc cggtcgatcg acagactcac
     5401 atcatcgaga acgacgttgg aaccgaaggc cttcttgacg tggtcgatct cgaccaggtg
     5461 cccgttactg gattgagccg acatttaggt tgcggagcct actcgtctag aggtacccga
     5521 gggattccag ccttttcctg atggcttcga tctcgtccgg cttcaggg
//
LOCUS       DATMED010000374         3467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1837925, whole genome shotgun sequence.
ACCESSION   DATMED010000374 DATMED010000000
VERSION     DATMED010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837925"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VNP73_09730"
     CDS             <1..314
                     /locus_tag="VNP73_09730"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWL66239.1"
                     /translation="AAGAMMALVGLPGLPGVASRDDLDKITEGPWKPTDLRPTAFHPM
                     GTARMGRKGRSVVDSWGEHHDVKGLYVSDGSIFPTCVAVNPQVSIMAFATKTAERIAS
                     N"
     gene            complement(328..2052)
                     /locus_tag="VNP73_09735"
     CDS             complement(328..2052)
                     /locus_tag="VNP73_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66240.1"
                     /translation="MIRPTFPPQIRRGVARYVPVIVTVVLVAAAVWLAPPVETTSVRQ
                     SDDSAISNGPAEAPADLAAPGTTGTSNGSTNGGEQTQASGSGERALPENCDPATGKIK
                     FPSVYSPPCVYPVSDNGGATYQGVTKDTIKVVVYDPQSDPAVDALLRSVGAADTREQL
                     IETYRGWLNLFNTYYETYGRRVVLEVIEASGPTDDDAAARADAIKVATQMKPFAVFGG
                     PSPFVDELARRQIICITQSQRPNTYFTDRAPFVWGTQMSSSQVFFHLAEYIGKRLRGN
                     AVHAGDAAYRTKPRKFGIVFSDTAEQVYRPGIEYLERELKKYGVALADKVAYEPDINT
                     AQEQARTIVARLKDKGVTSVLFSGDGLAPIFFTQEATSQSWFPEWVLSGGSLVDTNFL
                     GRTYDQRQWSHAFGISQLWIRPPKPVVEPYYQYVWHHGRPPPAVTTYEILYQEPLVLF
                     TGIHMAGANLNPNTFRDGLFAYPPSGVGTITQLQRSFGNHGIWPQPDYTGFDSVAEVW
                     WDPKARGAAEQGTVADGMFRFVDGGKRYTPGQWPSTPPKVFTTDGTRIDYDALPPSDK
                     YPTYPPKR"
     gene            complement(2049..2798)
                     /locus_tag="VNP73_09740"
     CDS             complement(2049..2798)
                     /locus_tag="VNP73_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66241.1"
                     /translation="MLDRFRELRPSEFGEALSRNRQFVVGLVVVVAAVWFFPGKPAPQ
                     LLSGARPGAPTGIVSTAPPIQEGGSLAPISNPGGPPPAAAFDDSIIGGLPPETEPEPA
                     AVCSTDALADLLDTVRDPIGELLGSPIPGAAIRDLAAIAAGCSTEDPTGPVLDLALEF
                     AGLVPDTGLDTIDLPDLPGLPPVDVPDEVTDALGPLGDTIREGCGTLSLVAVVFAVLP
                     AAAHLPVGGSDLTQFLGPVSVFCSLFDEDAS"
     gene            complement(2804..>3467)
                     /locus_tag="VNP73_09745"
     CDS             complement(2804..>3467)
                     /locus_tag="VNP73_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWL66242.1"
                     /translation="DLSADARARIGLARSFQDARLFPGLTVEEAIAVGLDREVGVKDP
                     LVISLGLDSAATSERETKQRVDELVELMGLDAFRNKFIAELSTGTRRIVDLACVLGHR
                     PSVILFDEPSSGIAQREAEALAPLLSKIRDDTGAALVVIEHDIPLVTSISDRMVALDL
                     GRVIAEGSPRDVVRDPAVVTSYLGTNDAAITRSGKRSGGATRSTRRTTAGKRKPRKRV
                     RT"
BASE COUNT          637 a         1069 c         1130 g          631 t
ORIGIN      
        1 tggcggctgg cgccatgatg gcgctggtgg gcctaccggg cttgcccggc gttgcttcgc
       61 gcgacgacct ggacaagatc acggaaggcc cttggaaacc gacagatcta cgtcccactg
      121 cattccaccc gatgggaaca gcacggatgg gacggaaggg acggagcgtc gtcgactcct
      181 ggggcgagca tcacgacgtc aaaggactct acgtttccga cggttcgatc tttcccacct
      241 gcgtggccgt caacccacaa gtgtcgatca tggccttcgc caccaagacc gccgagcgaa
      301 tcgcaagtaa ctaactaccc actgctacta gcgcttcggt ggataggtgg ggtacttgtc
      361 gcttggcgga agcgcgtcgt agtcgatgcg cgtgccgtca gtcgtgaaga cctttggtgg
      421 cgtgctgggc cattggcccg gcgtgtagcg cttacctccg tccacgaatc tgaacatccc
      481 gtcggcaaca gttccctgtt cggccgcccc tcttgctttg gggtcccacc agacctctgc
      541 cacggaatcg aagcccgtgt agtcgggttg cggccagatc ccgtggttcc cgaacgagcg
      601 ttgaagctgg gtgatcgtgc cgacgcccga aggtgggtag gcgaacaggc cgtcccggaa
      661 cgtgttcggg ttcagattcg cgccggccat gtggatcccg gtgaatagca ccagcggctc
      721 ttggtagagg atctcgtagg tcgtgactgc aggcggagga cgcccgtggt gccagacgta
      781 ttggtaatag ggctcgacca ccggcttcgg aggccggatc cacaattggc tgatgccgaa
      841 agcgtgactc cactgccgct ggtcgtatgt gcgtccgagg aagttggtgt ccacgagcga
      901 gccgccggac agtacccact cggggaacca gctctgcgat gtcgcttctt gcgtgaagaa
      961 gataggcgca agtccatcac cgctgaagag gaccgacgtg acgcccttgt ccttcaatcg
     1021 cgcgacgatc gttcgtgcct gttcctgtgc ggtgttgatg tcgggttcgt atgcaacctt
     1081 gtcggctaaa gccacgccgt acttcttcag ctcgcgctcc agatactcga tcccaggtcg
     1141 gtagacctgc tccgcagtgt cggagaaaac gatcccgaac ttgcgcggct tggtccgata
     1201 cgccgcgtcg ccggcatgaa cggcgttacc gcgcaaacgc ttaccgatgt actcggcaag
     1261 gtggaaaaag acctgagacg agctcatctg cgtaccccaa acgaacggcg cgcggtcggt
     1321 gaagtaggtg ttgggccgct gagactgtgt gatgcagatg atctggcgtc gcgcgagctc
     1381 gtcgacgaag ggcgacggcc ccccgaaaac ggcgaacggt ttcatctgag tggccacctt
     1441 gatcgcgtct gcgcgagcag cggcgtcgtc gtcggtgggt ccggaggcct cgattacctc
     1501 gaggactaca cgtcgcccgt aggtctcgta gtaggtgttg aagaggttta gccatccgcg
     1561 gtacgtctcg atcagctgct cgcgcgtgtc ggccgcaccg accgatcgga gcaaggcgtc
     1621 gaccgccgga tccgactgag ggtcgtagac gaccaccttg atcgtgtcct tcgttactcc
     1681 ctgatacgtc gcaccgccgt tgtcgctcac ggggtagacg caaggcggtg aataaacgga
     1741 tgggaacttg atctttcctg tagccggatc gcagttctcc ggaagtgccc gttcgcccga
     1801 gcccgaggcc tgcgtttgct cacctccgtt tgtcgatccg ttacttgtcc ccgtggtgcc
     1861 cggcgcggcc aggtcggctg gggcttcggc cggcccgttc gaaatcgccg agtcgtcgga
     1921 ctggcgaacg gatgtggtct cgacaggcgg agcgagccag accgccgcgg ccacgagcac
     1981 gacggtaacg atgaccggga cgtaacgggc gacgccgcgg cgtatctggg gcgggaaagt
     2041 tggccggatc acgaagcgtc ctcgtcgaag aggctgcaga agacggaaac cggcccgagg
     2101 aactgcgtca ggtcggagcc gcccacgggc aagtgggccg cggcgggcag cacggcgaac
     2161 accacggcga ccaaagacag ggttccgcag ccttcacgga tcgtgtcgcc caggggtccg
     2221 agcgcatctg tgacttcgtc gggcacgtca accggaggca gccccggaag atccggcaga
     2281 tcgatcgtgt ccagacccgt atccggaacg agtccggcga actcgagcgc gaggtcgagc
     2341 accgggcccg ttgggtcctc ggtgctacac cccgcggcta tagccgccaa gtctcggatc
     2401 gccgcgccgg gaatcgggga gccgagcagc tcgccgatgg ggtctcgaac cgtatccagc
     2461 aagtcggcca gcgcatcggt cgaacacacg gcagccggct ccggttcggt ttccggcggc
     2521 agcccaccga taatcgaatc gtcgaaggcc gcggccggcg gcgggccacc tgggttcgat
     2581 atcggggcga gggaaccgcc ttcctggatc ggcggcgctg tggaaacgat tccggtcgga
     2641 gcgcccgggc gcgcaccgga cagaagttgg ggcgccggct tgccggggaa gaaccacacg
     2701 gcagccacca cgactacgag tccaacgacg aattggcgat tgcgcgagag cgcttctccg
     2761 aactcggacg gacgcaattc gcggaatcga tcaagcatct ttctcatgtt cgcactcgct
     2821 tacgtggttt gcgcttgccg gccgtcgtcc tgcgcgtgct tcgcgttgcg cctccagatc
     2881 gtttgcccga ccgtgtgatc gctgcgtcgt tcgttcccag ataagaggtc acaaccgcgg
     2941 ggtcgcgcac aacgtcgcgt ggagaccctt cggcgattac acgcccgaga tctaatgcca
     3001 ccattcggtc cgatatagag gtaacgagcg ggatgtcgtg ttcgataact accagcgcgg
     3061 ctccagtatc gtcccggatc ttcgagagca agggcgccag cgcctccgct tcgcgctgag
     3121 cgatcccaga ggaaggctcg tcgaaaagga tcaccgatgg ccggtgaccc agcacgcacg
     3181 ccagatcgac gatacgacgg gttcccgtcg acagctcggc aatgaacttg tttcggaaag
     3241 catcgagacc catcagctcg acgagctcat cgacgcgctg cttcgtttct cgttccgatg
     3301 ttgccgccga gtccagaccg agcgagatga cgagtgggtc tttgactccg acttcgcgat
     3361 caaggccgac ggcgatcgcc tcctcgacgg tcagaccggg gaaaagacga gcgtcctgga
     3421 acgatctcgc caatccgatg cgagcccgcg cgtcggcgga gaggtcg
//
LOCUS       DATMED010000375         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_315063, whole genome shotgun sequence.
ACCESSION   DATMED010000375 DATMED010000000
VERSION     DATMED010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315063"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VNP73_09750"
     CDS             complement(<1..208)
                     /locus_tag="VNP73_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66243.1"
                     /translation="MNALKVVRRNQLVLWRQWRWSLFLSVLSPVMFLSAMGLGIGGLV
                     ERSSPDAFGSSGYVAFFATGMLAVT"
     gene            complement(205..1122)
                     /locus_tag="VNP73_09755"
     CDS             complement(205..1122)
                     /locus_tag="VNP73_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL66244.1"
                     /translation="MLIKARSLTKSFGDLVAVDSIDFEVREGEAFGFLGPNGAGKTST
                     MRMIGCMSPPTAGSLTVMDLDPVAQASEIKARIGVVPQIDNLDIEITVRENLEIYARY
                     FDVPRNEAKRRAQELLEFVQLTDKASSKVEPLSGGMKRRLTIARGLINQPDLLLLDEP
                     TTGLDPQARHLVWDRLYRLKQQGVTLVITTHYMDEAEQLCDRLVVMDKARIVAEGSPR
                     DLIARYCTTEVLELRLTDEARGRLADSVDGIAKRVEWLPDRVLLYTDQGDAALGAVHE
                     RGVTTESALVRRSTLEDVFLTLTGRTLIE"
     gene            1276..2316
                     /locus_tag="VNP73_09760"
     CDS             1276..2316
                     /locus_tag="VNP73_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66245.1"
                     /translation="MSRTRLALIAALLMFAAALPAGVARAQTDQIVGLWFFGDIEVEA
                     IPNASGGFDGIVRKTVEGTCFDVDEQIWFITAKKSDNVYTGSHSFYYNEGCGYAGQGK
                     TTWTLSEAGSRVDLLGESPTGDGKKDGYTMYKRIDCTDENTICGGETNDNIQGTSEND
                     TIYAGGGDDEIDGGGGEDVIVGGEGNDTLTGGAANDQLAGGPGNDVLVGDGEIVDEGP
                     LLSKYTLAFSLQDDAAGADTLEGGAGNDSVDGGDGDDNVQGGGGTDSVVGGTGNDKMD
                     GQAGKDDLNGGQGGDVLNGGGGTDKMNGAGGTDTCVLDTRQDSTRSCERKKLNFKRNG
                     PVPVPMRFLFGI"
BASE COUNT          462 a          695 c          754 g          411 t
ORIGIN      
        1 aggtgacggc gagcatcccg gtagcgaaga aggcgacgta tccggagctg ccgaatgcat
       61 cggggctact ccgctcgacc aagccaccga tccccagtcc catcgccgac aggaacatga
      121 ccggagacag gaccgacagg aacagcgacc atcgccactg gcgccataag acgagttggt
      181 tgcgtcttac gaccttcagc gcgttcactc gatcaaagtc cgtccggtga gcgtgaggaa
      241 gacgtcctcg agcgtcgagc gtcgcaccag ggcgctttcg gttgtcactc ctcgctcatg
      301 cacggccccg agtgcggcgt cgccctgatc cgtgtacaga agcacccgat ccggcagcca
      361 ttcgaccctc ttcgcgatgc cgtccactga atcggccagt cgaccgcgtg cctcgtcggt
      421 gaggcgaagc tcgagcacct ccgtggtgca gtagcgagcg atgagatcgc gcggcgatcc
      481 ctctgccacg atgcgggcct tgtccatcac gacgagccga tcgcacagct gctcggcctc
      541 gtccatgtaa tgagtcgtga tcacaagcgt tacgccttgc tgtttgagcc ggtacagccg
      601 gtcccacacg aggtggcgtg cttgcgggtc aagaccggtg gtcggctcgt caagaagcag
      661 cagatcgggc tggttgatga gaccgcgagc gatcgtcagc cgtcgcttca tgcccccgga
      721 caaaggttcg accttgctcg aggccttgtc cgtgagttgc acgaactcga gcaactcttg
      781 agctcgccgc ttcgcttcgt tgcgaggaac gtcgaagtaa cgcgcgtaga tctcgaggtt
      841 ctctcgcacc gtgatctcga tgtcgaggtt gtcgatctgc ggcacgactc cgatccgtgc
      901 tttgatctcg gaagcctgcg cgaccggatc gagatccata accgtgagag agcccgccgt
      961 cggcggggac atgcacccga tcatccgcat cgtcgacgtc tttccggccc cgttggggcc
     1021 gaggaacccg aaggcttcgc cctcgcggac ctcgaagtcg atcgagtcga ccgctactag
     1081 gtcgccgaac gatttcgtca gcgaacgtgc cttgatgagc atgatgcgaa ccgtattccc
     1141 ccgtactcgg ttccgattcg caccctttcg gtgggtctga acggtgtcgg tcgctagcta
     1201 gacacacaaa ccccttgcat gcttcgtccg tcggtgtctg acttgaatcg gaagttgccg
     1261 taaagggggt atgggatgtc cagaactcgc ttggcgttga tcgcggcgct actcatgttc
     1321 gcggctgctc ttccggccgg ggttgctagg gctcagaccg accagatcgt cgggctgtgg
     1381 ttcttcggcg atatcgaggt ggaagcgatc ccgaacgcga gcgggggctt cgacggcatc
     1441 gtccgcaaaa cggttgaagg cacctgcttc gacgtggacg agcagatctg gttcatcacc
     1501 gccaagaaga gcgacaacgt ctacacgggg tcgcactcct tctattacaa cgagggctgc
     1561 ggctacgccg gtcaaggcaa gacgacctgg accctgtccg aggccggctc acgcgtcgac
     1621 ctccttggag aatccccgac gggcgatggg aaaaaagatg gctacacgat gtacaagcgg
     1681 atcgattgca ccgacgagaa cacgatctgc ggcggggaga ccaacgacaa catccaaggc
     1741 acttctgaga acgacacgat ctacgcgggc ggcggcgacg acgagatcga tggcggtggc
     1801 ggtgaagacg tgatcgtcgg gggagagggc aacgacaccc tgaccggtgg ggccgccaac
     1861 gaccagttgg caggtggtcc gggcaatgac gtactcgtgg gcgacggcga aatcgtcgac
     1921 gaggggccgc tcctctcgaa gtacacgttg gctttcagcc tgcaggatga cgccgccgga
     1981 gcggacacgc tcgaaggcgg cgcaggcaac gattcagtcg atggtgggga cggcgacgac
     2041 aacgtccaag gcggcggcgg aaccgattcg gtcgtcggtg ggaccggcaa cgacaagatg
     2101 gatggccagg cgggcaagga cgacctcaac ggcggccaag gcggagacgt cctaaacggg
     2161 ggcggaggca ccgacaagat gaacggtgcc gggggcacgg acacgtgtgt tctggatact
     2221 cgccaggatt cgacccgttc gtgtgagcgc aagaagctga acttcaagag gaacgggccg
     2281 gtgccggtac cgatgaggtt ccttttcggc atctgacccg cg
//
LOCUS       DATMED010000376         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4911705, whole genome shotgun sequence.
ACCESSION   DATMED010000376 DATMED010000000
VERSION     DATMED010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4911705"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VNP73_09765"
     CDS             <1..307
                     /locus_tag="VNP73_09765"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWL66246.1"
                     /translation="LFPITNAAASPMRYTFDQDEYKAVSDELWARGWDLGALYHSHTR
                     TEAYPSPTDVRDAREDVPYVIVSLASEPPVIRAFRIVKENWQAPSGDVEEIPVEVTG"
     gene            321..599
                     /locus_tag="VNP73_09770"
     CDS             321..599
                     /locus_tag="VNP73_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWL66247.1"
                     /translation="MAVTVRIPTVFRKFTGNAATVEVGPGTIADLIDQLEQMHPGFRE
                     QMLAEDGQLHRFVNVYVNDEDARYLEKLDTKVAEGDTVSILPSVAGGA"
     gene            596..1537
                     /locus_tag="VNP73_09775"
     CDS             596..1537
                     /locus_tag="VNP73_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HWL66248.1"
                     /translation="MRYKSILDTIGNTPLVQVPHLTPVPDVRLWVKLEGENPTGSVKD
                     RIALAMVEAGEASGELTKDKILLEPTSGNTGIALAMVTSIRGYRFTAVMPDNASAERV
                     QLLKAFGAEIVFSEGAKGTNGSVALAQEMSSDDRYYMPFQYGNPANVDAHYRGTAREI
                     IEDLPEVKAFVAGLGTGGTLMGNGKRLREHNPDVKIVAAEPELGELVYGLRSLEDGYI
                     PPIFDPDMLDGKVMVKARDSILWTRELLRKEGIFSGISSGAVIWVAQRVAERIAKDGG
                     GDVVCLLPDGGWKYLSTEAWTQDIDTAEAQVEKVLWW"
     gene            1531..2337
                     /gene="murI"
                     /locus_tag="VNP73_09780"
     CDS             1531..2337
                     /gene="murI"
                     /locus_tag="VNP73_09780"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWL66249.1"
                     /translation="MVGDARPIGMFDAGLGGLTVARAVIDLLPHENLIYFGDTARCPW
                     GPRDPAEIRRFSLEIVDLLVDQGVKLIVVACNSAETAAFYESTGRYEVPIVTVIEPGV
                     RAALAATRNRRIGVIGTEATVSSGAYESAIQKTRANVEYFVRACPKFVEFVESGDTTS
                     DALIAAAEEYLLPLKEEGIDTLVLGCTHYPLLSGVIQYVMGDQVVLVSSAEPTANEVY
                     ARLLDDDMLSRSDEPGHLRFVLSGPPDDELGRRFLGPEFRNVESHAWGSE"
     gene            2334..3077
                     /locus_tag="VNP73_09785"
     CDS             2334..3077
                     /locus_tag="VNP73_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWL66250.1"
                     /translation="MTISLTVLGTSGMYATVERAASGYLLDVDGTKICVDMGAGTWRN
                     LLAEISYGEIAGILLSHCHPDHVTDVFQAYHARRYGQPDPMPKIPLWAPAETLERVLG
                     FLPEMVEAFDLRTIKAGATMDLAGSSLSFIDMAHPAETVGMRFEQDGKVLAYSADSGE
                     AADFEALASGADVFLCEATLQEADEPWEGHLSGAQAGRIGAAAGVGKMVLTHLPPGRD
                     HEVTLSEARGAASDLKLELAADGMHIDVV"
     gene            3087..>3333
                     /locus_tag="VNP73_09790"
     CDS             3087..>3333
                     /locus_tag="VNP73_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013372747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWL66251.1"
                     /translation="MSRVDGRGAADLRPVTLTPAFMPYAEGSCFVEMGETKVICTATI
                     EDGVPRWMKGQGAGWVTAEYSMLPRSSPERVTREVSKG"
BASE COUNT          633 a          981 c         1097 g          622 t
ORIGIN      
        1 gctgtttccg atcaccaacg ctgcggcgag tcccatgcgg tacaccttcg accaggatga
       61 atacaaagcc gtgtcagacg aactctgggc cagaggctgg gatctcggtg ccctctacca
      121 ctcgcatacc cgaaccgagg cttatccctc tccgacggac gtcagggatg cccgcgagga
      181 cgtgccctat gtgatcgtgt cgctggcgtc cgagcccccg gtcatcaggg cgtttcgtat
      241 cgtgaaggag aactggcagg ccccctcggg tgacgtggaa gagataccgg tagaggtgac
      301 cggttgacta ccatgtgcct atggccgtaa ccgttcggat accaaccgtt ttcaggaagt
      361 tcacgggtaa cgccgccacc gtcgaggtag gccccgggac gatcgcggat ctgatcgatc
      421 agctcgaaca gatgcatccc gggttccggg aacagatgct cgcagaagat ggccagctgc
      481 atcgtttcgt caacgtctat gtcaatgacg aggacgctcg ctatctcgag aaactggata
      541 cgaaggttgc ggaaggcgac accgtttcta tcctgccctc tgtagccggc ggcgcgtgag
      601 atacaaatcg atactggaca ccatcggcaa cacgccgttg gttcaagtac ctcacctgac
      661 tcccgttccc gacgtccgtt tgtgggtcaa gctcgaaggc gagaacccga ccggatcggt
      721 taaggatcgg atcgcgctgg ccatggtcga ggccggcgag gcttcaggcg aactcacgaa
      781 ggacaagatc ctcctcgaac ctacttcggg gaataccggt atcgcgctcg cgatggtcac
      841 gagtatccgc gggtatcgct tcaccgcggt gatgcccgat aacgccagcg cggaacgggt
      901 ccagctgctc aaggcttttg gcgccgagat cgtgttctct gaaggagcca aggggaccaa
      961 cggttcggtt gccctcgcac aagagatgtc gagcgacgac cgctattaca tgcctttcca
     1021 gtacggcaac ccggcgaacg tcgacgctca ttaccgcggc actgcgcgcg agatcatcga
     1081 ggatcttccc gaagtgaagg ccttcgtcgc gggtctcgga accggcggga cgttgatggg
     1141 gaacgggaag cggctgcgcg agcacaaccc cgacgtgaag atcgtggccg ctgagcccga
     1201 gctgggtgag ctcgtctacg gactgaggtc gcttgaagac ggttacatcc cgccgatctt
     1261 cgatcccgac atgctcgacg ggaaagtcat ggttaaggcg cgcgactcga tcctgtggac
     1321 gcgtgagctt ctgcggaagg aagggatctt ctccggcatc tcctccggcg cagtgatctg
     1381 ggtggcccag cgggtggcag agaggatcgc caaggacggt ggcggggatg tcgtgtgcct
     1441 attgcccgac ggcggatgga agtacctgtc gaccgaggcg tggacgcagg acatcgatac
     1501 ggcggaggcg caggtcgaaa aggtcctctg gtggtaggag acgctcggcc gatagggatg
     1561 ttcgatgccg ggctgggagg actaactgtc gcccgtgccg tgatcgacct cttgccgcac
     1621 gagaacctca tctacttcgg ggacacggcg aggtgcccgt gggggccgcg agatccagcg
     1681 gagatccgcc gcttctccct cgagatcgtc gacctattgg tcgaccaggg cgtgaagctc
     1741 atcgtcgtcg catgtaactc ggccgagacg gccgctttct acgagtcgac cggccgctac
     1801 gaggtgccga tcgtgaccgt tatcgagccg ggcgtccgcg ccgcactcgc cgccacccgt
     1861 aaccggagga taggggtgat cgggacggag gcgacggtta gctcgggggc ctacgagtcg
     1921 gccatccaga agaccagggc caatgtcgag tacttcgtcc gagcttgccc caagttcgtc
     1981 gagttcgtcg agagcggcga cacgacctcc gacgccctga tcgcggccgc cgaggaatac
     2041 ctacttccat tgaaagagga aggcatcgat actctcgttc tcggttgcac gcactatccg
     2101 cttctatccg gggtgatcca atacgtcatg ggcgaccaag tggtgctggt ttcgagcgcc
     2161 gagccgacgg ccaacgaggt ctatgcgcgg ctgcttgacg acgacatgct gagccgctcc
     2221 gacgagcccg gccacttgcg gttcgtgctg tccggccccc ccgatgacga gctcggtcga
     2281 cgtttcctgg ggcccgaatt ccgcaacgtc gaatcacatg cctggggaag cgaatgacga
     2341 tctctctcac ggttctcgga acatctggga tgtatgcaac cgtcgagcgc gcggcgtccg
     2401 gttacttact cgatgtcgat ggaacgaaga tctgcgtcga catgggggct gggacgtggc
     2461 gcaacctcct cgccgagatc agttacggcg agatcgctgg gatcctgttg tcgcactgcc
     2521 atcccgatca cgtcacggat gtcttccagg cgtatcacgc tcgtcgctac ggacagcccg
     2581 acccgatgcc caagatcccg ttgtgggctc cggccgaaac gctggagcgc gtgttgggct
     2641 tccttccgga gatggtggag gcgttcgacc tgcgcacgat caaagccggt gccacgatgg
     2701 atctagccgg ctcgtccctc tcgttcatcg atatggccca cccagccgag actgtgggta
     2761 tgcgcttcga gcaagacggg aaagtgctcg cttactcggc cgactccggg gaggcggccg
     2821 atttcgaggc attggcttcg ggagccgacg tcttcctgtg cgaagcgaca ctgcaagagg
     2881 cggacgaacc gtgggagggt cacctttctg gtgcacaggc gggtcgcatc ggggccgcgg
     2941 ccggggtagg gaagatggtg ttgactcacc ttccgccggg gcgggatcac gaggtgactt
     3001 tgtccgaggc gagaggtgcg gcgagcgatc tcaaactcga acttgccgcc gatgggatgc
     3061 acatagatgt cgtctaaggg ggacagatga gccgagtgga tggccgggga gcggcagatc
     3121 ttcgacccgt caccctcacc ccggctttca tgccgtacgc ggaagggtcc tgtttcgtag
     3181 agatggggga aacgaaggtc atctgcacag cgacgatcga ggacggggtg ccgcgttgga
     3241 tgaaggggca gggcgccgga tgggtcaccg ccgagtactc catgcttccg aggtcgtcac
     3301 ccgaacgcgt gacgcgtgag gtgtccaagg gcc
//
LOCUS       DATMED010000377         7180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4911829, whole genome shotgun sequence.
ACCESSION   DATMED010000377 DATMED010000000
VERSION     DATMED010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4911829"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VNP73_09795"
     CDS             <1..1021
                     /locus_tag="VNP73_09795"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWL66252.1"
                     /translation="GREAVSFTGSQAGIVTDTRHGRARIVDVRAQRVLEALDVGKIAI
                     VAGFQGVSTDYDVTTLGRGGSDTSAVALAAALQADACEIYTDVDGVFTADPRIVPEAR
                     KLHAVSYEEMLELAASGAKVLMLRSVEYARNHGVLIHVRSSFTREEGTWVREEDARME
                     QAIISGIAHDTSEAKVTILGVPDQPGVAARVFRPLADEGVNVDMIVQNASADGRTDIF
                     FTLPKDDLHRAGPLLKLISSDVGAVDVQTDPDIAKVSLIGAGMKSHPGVAADMFDALA
                     EAGINIEIISTSSIRVSCVVRASDVERAVKAIHDRFQLSEEAVLREQHPATATDQLRA
                     LEGNA"
     gene            1018..2046
                     /locus_tag="VNP73_09800"
     CDS             1018..2046
                     /locus_tag="VNP73_09800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWL66253.1"
                     /translation="MRLVVVGATGAVGTVMMDILEERDFPADEIIPVASPRSAGRKLD
                     YQDRQLEVVALDESVFDGADLALFDVPDELSAQWAPVAVRQGAIAIDNSGAFRMDDDV
                     PLVVPEVNPRAVEQRPRGIIASPNCTTLAMVVPLAALHRAAGVERLVLASYQAASGAG
                     RPGSDELWDQTEVAAKNPDDVREGKAGRILEGGEYFVHPIAMNVVPRCGSVKEDGYTS
                     EELKLGNETRKIMDLPDLEVTATCVRVPVVVGHGVAVHARFGRAIDVDEARSLLSSAP
                     GVELVDDLSADVYPTPLEAAGKDACFVGRIRRDLHDERALAFFCVADNLRKGAALNTV
                     QVAELLVR"
     gene            complement(2067..2144)
                     /locus_tag="VNP73_09805"
     tRNA            complement(2067..2144)
                     /locus_tag="VNP73_09805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2107..2109),aa:Pro,seq:cgg)
     gene            complement(2172..3800)
                     /locus_tag="VNP73_09810"
     CDS             complement(2172..3800)
                     /locus_tag="VNP73_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWL66254.1"
                     /translation="MRALRLGVALALLVAMTVPPAVGADVPGIKTSPPGQISVVTINA
                     RQYPILGIRRFRAMYQLSRALRRRPEAFDGGFNGAVHAPDVIVLQEIRPSNLEIFEHI
                     LRQRFGVKYVIVGPADAAAAILAHPETVSPVGEVLLLQDACSTPADDRYPQRQYPLAR
                     FIEQPSGASFAIVGVHFPKASVTQNADCLARNVDQFRTLFEVEQTPTIIAGDFNRRPV
                     STPFECDVDERSDPLPWYANLTAPDNGGRQYLDAVREFNRSHSTSMAFEWTHEHKAKK
                     PDCTGNVFIRRSRIDYIFVADGKIADAHADHPGWGGEVPGSRRPGNFKYSDHRWVSAR
                     VVLSGPRKPIAPVVAADAGGLIRVSWEPVNAASQYILYRALGERAFGVAGKLPAETTA
                     FEDLGTQHGRTYRYAVAAVGADGGQGVESFGNSAIADSLGPQVVGTDPLKGAIGVGPG
                     KAISVRFNEGIADESVGEGAIRLYVDGHTVRGFAFRKFPRLLRFNPNDPLKEGRLYTA
                     VVAYGLEDRLGNESERFAFSFRTVDPPKRRGRRR"
     gene            complement(3831..4700)
                     /locus_tag="VNP73_09815"
     CDS             complement(3831..4700)
                     /locus_tag="VNP73_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWL66255.1"
                     /translation="MTWLRRLVALGAAIAAYSFVEARSYRLTRLVVPVRSPIGHLTVL
                     HLSDTHLWGRNAALVRFLERLPELVGTPDLVIATGDFLEGNEGIGPAVAGLAGLEARL
                     GRYYVLGSHDYFQSERPGFARYLTGNRRAPTSKPAATDELRKGLEAKGWVSVMNDLVR
                     VESPFGPIQISGVDDPYIHRHTTQHIRRREDAVLALGLTHAPDVVSEWILENFDVVFA
                     GHTHGGQIRIPAVGALVTNCSLPTGLAKGLHRVGPGWLHVSPGLGTSHFSPVRFACPP
                     EATLLDLVGESPS"
     gene            4748..6328
                     /locus_tag="VNP73_09820"
     CDS             4748..6328
                     /locus_tag="VNP73_09820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWL66256.1"
                     /translation="MRLSLRWKIAGGFALMLVLIALLGWVTLSLFLSLRTIQRNVFDN
                     AIPGLITIDEIVRSYTAQSAAVRGYLIGSQPSLLDQYRREVAIADVWEERGERLFRSS
                     EERSLLDDLRVAGGEFQKLVDRQVIPRASRGERTQAFRVLGQEGTPLITEIETLGGLL
                     RTQQDRLRIESERDVRSASNNALILLGAVIAGSLALGGMLAVLLPRRLVANLSRLVDA
                     ARAIERGDLDQKISVESRDEVGELAERFNEMQVGLKRLQQLALQDRELEIAASIQRNL
                     LRRGVPHMTEARVVPLQRQANLVGGDWYDVDLIGRTLTVVVGDASGKGIAAALMATVA
                     LSALRAERGKVADPKHVLEAANRALRDASDPESFTTVIYSTLDLDTGATSWINMGHHS
                     PFVLRAAESPDHATQGYYLEGPRNRALGWFEDPGLTENHARLAPGDRLVLFTDGFLEA
                     KSQEGEVFGEHRLAEVLIKLAPLDGDALEHELVAEVERFAAGKLDDDLTMIIIEWVGT
                     REGVPDRASGEEPWRSKR"
     gene            6310..6663
                     /locus_tag="VNP73_09825"
     CDS             6310..6663
                     /locus_tag="VNP73_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWL66257.1"
                     /translation="MAQQKIDIDITNEGDYRVFKPSGDLDVYTVGSLRDAIGAVIEEE
                     NPRIVVDLDAVPFMDSSGLGALMGGVRRLREAGGDLAIACTREQHLKLFTITGFGEGV
                     SIAPTVDEAAKGFKA"
     gene            complement(6660..6788)
                     /locus_tag="VNP73_09830"
     CDS             complement(6660..6788)
                     /locus_tag="VNP73_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66258.1"
                     /translation="MTSAQLILISVTSAMVAVFVMLLVATIRRARARDEDEKDSAD"
     gene            6807..>7180
                     /locus_tag="VNP73_09835"
     CDS             6807..>7180
                     /locus_tag="VNP73_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66259.1"
                     /translation="MDISHFAQAPSDASVFEDTFRFAAYTIARLLGFSAHAILFGLVP
                     LLLLVIRPTFSALRGPEWDRGRERVAARLEGFVQAALVAAALITALTVTLQGALIAEA
                     TGGTFSLDSVLDTLETTFGQWH"
BASE COUNT         1298 a         2177 c         2372 g         1333 t
ORIGIN      
        1 cgggcgcgag gcggtttcgt tcaccggctc ccaggccggc atcgtgaccg acactcgcca
       61 cggcagagct cggatcgtcg atgtgagggc gcagcgcgta ctcgaagcgc tcgatgtcgg
      121 gaagatcgcg atcgtcgccg gcttccaagg ggtatccacg gattacgacg tgacgacgct
      181 ggggcgcggt gggtccgata cgagcgcagt cgcccttgct gcggcgctgc aggctgacgc
      241 ctgcgagatc tacacagatg tcgacggcgt tttcacggca gatccaagga tcgttccgga
      301 agctcgcaag ctccatgcgg tttcgtacga agagatgctc gaactcgcag catcgggcgc
      361 caaagttctg atgttgcgca gcgtcgaata cgctcggaat cacggagttc tcatccacgt
      421 tcggtcctcc ttcacacgcg aagaaggcac ctgggtgaga gaggaggatg cgcggatgga
      481 acaagccatc atctccggga tcgcccacga cacttcggag gcgaaggtca cgatcctcgg
      541 cgtgccggac cagcccggcg tggccgcccg cgtcttcaga cctctggcgg acgagggcgt
      601 gaacgtcgac atgatcgtgc agaacgcatc ggccgacggt cgaacggaca tcttcttcac
      661 acttcctaag gacgacctgc accgggccgg accgctcctg aagctgatct cttccgatgt
      721 gggcgcggtc gacgttcaga ccgatcccga catcgccaag gtttccctga tcggagccgg
      781 catgaagagc catcctggtg tggctgccga catgttcgac gcgctggccg aggcgggtat
      841 caacatcgag atcatctcca cgtcgtccat ccgtgtgtcg tgcgttgtca gagcgtccga
      901 tgtcgaacga gcggtcaaag ccatccacga ccggttccag ctgtcggagg aggccgtgtt
      961 gagagaacag cacccggcga ccgctacgga ccagcttcga gctctggagg gcaacgcatg
     1021 agactggtgg tcgtaggtgc taccggcgca gtcggcaccg tgatgatgga catcctcgag
     1081 gaacgcgact tccccgccga cgagatcatc cccgtcgcct ctcctcgctc ggcagggcgg
     1141 aagctcgatt accaagatcg acaactcgag gtcgtcgccc tggacgaatc ggttttcgac
     1201 ggggcagatc tggcgctctt cgatgtgccc gatgagctat cggctcagtg ggctcccgtt
     1261 gccgttcgac agggtgcgat cgcgatcgac aactcgggag cgttccggat ggacgacgac
     1321 gtcccactgg tcgtccccga agtcaatcct cgagccgtcg agcaacgacc gcgcgggatc
     1381 atcgccagcc ccaattgcac gaccctggcg atggtcgttc cactcgcggc actccatcgt
     1441 gcggcagggg tcgagcgcct cgttctcgcc tcgtatcagg cggcctcagg tgccggtcgc
     1501 cccggttccg atgagctctg ggatcagacc gaggtggctg ccaaaaaccc cgatgacgtg
     1561 cgggaaggca aggccgggcg gatcttggag ggtggcgagt acttcgtaca tccgatcgca
     1621 atgaacgtcg tgccccggtg tgggtcggtc aaggaggacg gttacaccag cgaggagttg
     1681 aagctgggga acgagacgcg caagatcatg gacctccccg atctcgaggt cactgcgacc
     1741 tgcgtgcgtg ttccggtggt ggtcggccac ggtgttgcgg tgcacgcgcg ctttgggcga
     1801 gcgatagatg ttgacgaggc tcgttcgctg ttgtcctcgg cccccggtgt ggaactggtc
     1861 gatgacttgt cggcggatgt gtatccgact cctctggagg cggcgggcaa ggatgcgtgt
     1921 ttcgttggac gcatccgccg cgatcttcac gatgagcgag cgctcgcgtt cttctgcgtt
     1981 gcggacaacc tgcgcaaggg tgctgccctc aacaccgttc aggtagcgga gctgttggtc
     2041 cgctagagat cttcagctca acatcttggt cgggaagggg ggatttgaac ccccgacctc
     2101 cgcgtcccga acgcggcgcg ctaaccaagc tgcgctactt cccgaatgtg ccaatgctac
     2161 aggtcccggt tctagcgccg gcggcccctt cgcttgggcg gatcaaccgt gcggaagctg
     2221 aaagcgaacc gttcggactc gttaccaagc cggtcttcga gtccgtatgc gaccacggcg
     2281 gtgtagagac gcccttcctt taggggatcg ttcggattga atcgaaggag gcgcgggaac
     2341 tttcggaacg cgaagcccct cacggtgtga ccgtcgacgt agaggcggat ggcaccttct
     2401 ccgacgctct cgtcggcgat cccctcgttg aagcgcacgg agatcgcctt ccccggcccc
     2461 acaccgatcg cccctttgag agggtcggtt cccacgacct ggggtccaag tgagtcggcg
     2521 atggcggagt ttccgaacga ctcgacgccc tgacccccgt cggcacccac ggctgcaacc
     2581 gcatagcgat aggtgcgacc gtgctgggtg ccgaggtctt cgaaggccgt cgtttcggca
     2641 gggagtttcc cggctacacc gaaggctctc tcgcccagtg cccggtagag gatgtattga
     2701 ctcgctgcgt tcaccggctc ccagctcaca cggatcaagc cgccggcatc ggctgctacg
     2761 acgggcgcga tcggtttgcg agggccactc aacaccacgc gcgccgagac ccagcgatgg
     2821 tccgagtatt tgaaattgcc cggtctccgc gatcccggaa cctctccacc ccacccgggg
     2881 tgatcggcat gggcgtcggc gatcttgccg tccgcaacga agatgtagtc gatgcgcgat
     2941 ctcctgatga acacgtttcc ggtgcagtct ggtttcttgg ccttgtgctc gtgcgtccac
     3001 tcgaaggcca tcgaagtgct gtgcgagcga ttgaactcgc gcaccgcgtc cagatattgg
     3061 cgcccgccgt tgtccggcgc cgtcaagttc gcgtaccagg gaagaggatc tgatctctcg
     3121 tcgacatcgc actcgaaggg ggtagagacc ggtctccgat tgaagtcacc ggcgatgatc
     3181 gttggtgttt gctcaacctc gaatagcgtt cggaattgat cgacgttgcg agccagacag
     3241 tcggcgttct gcgtgaccga cgccttcggg aagtggaccc ccacgatggc gaaactcgct
     3301 ccgctcggtt gttcgatgaa acgagccagc gggtattgcc gttgcgggta ccggtcgtct
     3361 gcaggtgtcg aacacgcatc ctgcagcagc aggacctcgc ccacgggcga aaccgtttcg
     3421 gggtgagcca ggatggccgc cgccgcatcc gcgggcccga cgatcacgta cttgaccccg
     3481 aacctctgtc gcaggatgtg ctcgaagatc tcgaggttcg acggtcggat ctcttgcaac
     3541 acgatcacat cgggcgcgtg cacggccccg ttgaaaccac cgtcgaaggc ctcgggtctc
     3601 ctccgcagag cgcgagaaag ctgatacata gcgcggaacc ggcggatgcc gaggatgggg
     3661 tactggcggg cgttgatcgt gaccaccgag atctgccctg gtggtgacgt tttgatgcca
     3721 ggtacgtctg ctcccactgc aggtgggacg gtcatcgcta cgagtagggc gagcgcgacg
     3781 cccaaacgaa gtgcgcgcat ggagagagcg atgctaggcc acttgcatgg ttatgaagga
     3841 ctctctccaa ccaggtcgag caacgtcgct tccggggggc atgcgaagcg aacgggagag
     3901 aaatgactgg tgcccaggcc cggactgacg tgtagccagc cgggcccgac ccggtggaga
     3961 cccttggcga gcccggtagg gagactgcag ttcgtgacca gagctccgac cgcggggatg
     4021 cggatctgac cgccatgggt gtggccggcg aagaccacat cgaagttttc gaggatccac
     4081 tcagaaacga cgtccggtgc atgcgtgagg cccagagcga gaacggcatc ttcccggcgg
     4141 cggatgtgct gcgtcgtgtg cctgtggatg taggggtcgt cgacgccgga gatctggatc
     4201 ggaccgaagg gactctcgac ccgtaccaga tcgttcatga ccgagaccca acctttggct
     4261 tccagtccct tcctcagctc gtccgtagcg gccggtttgg atgtgggggc tcgacggttg
     4321 ccggtgagat agcgcgcgaa tcccggccgt tcggactgga aatagtcgtg cgagccaagg
     4381 acgtagtaac ggccgagccg agcttccaga ccggcgaggc cggccaccgc ggggccgatc
     4441 ccctcgttgc cctcgaggaa gtcacccgta gcgatcacga ggtcgggtgt ccccactaat
     4501 tcgggcaatc gctctaagaa ccgaacgagc gcggcgttgc gaccccacag atgggtgtcg
     4561 gagaggtgca acacggtgag gtggccgatc ggcgagcgta cgggcacgac cagtctcgtc
     4621 agcctgtagc tgcgggcttc tacgaaggaa taggcggcga ttgcggcccc gagggccacc
     4681 agccggcgga gccaagtcat cgttgtcgag gatactggga cctctacaac agggaggtaa
     4741 ccaggacgtg aggctctctc tccggtggaa gatcgccggt gggttcgccc tgatgttggt
     4801 tctgatagcg ctgctcggct gggtgaccct ctcgttgttc ttgtccctgc ggacgatcca
     4861 acgcaacgtg ttcgacaacg ccatcccggg gttgatcacg atcgacgaga tcgtgcggtc
     4921 gtacacggcg caatcggccg ccgtgcgcgg ctacctgatc gggagccagc cctcgctgct
     4981 cgatcagtac cggcgagaag tggcgatcgc cgacgtgtgg gaagagcgag gggagcgtct
     5041 gttcaggtcc agcgaggagc gttcgctgct ggacgacctg cgggtcgccg gaggggagtt
     5101 ccagaagctc gtggaccgac aggtgatccc gcgggcttcc cgcggcgagc ggacccaggc
     5161 tttccgagtg ctcggtcagg aagggacacc actgatcacg gagatcgaaa ccttaggggg
     5221 actgctccgg acacaacagg atcgcctccg catcgagagc gagcgggatg tgcgaagcgc
     5281 ttccaacaac gcgttgatcc tgttgggagc cgtcatcgcc ggttcccttg cgttgggcgg
     5341 aatgttggcg gtattgctgc cgcgacgtct ggtcgcgaac ctgtctcggt tggtcgacgc
     5401 ggctcgggcc atcgagcgcg gtgacctgga tcagaagatc tcggtggagt cgcgggacga
     5461 ggtcggggag ctcgcggaga ggttcaacga gatgcaggtt ggattgaagc ggctgcaaca
     5521 actggccctc caggatcgtg agctcgagat cgcggcctct atccaaagga acctgctacg
     5581 tcgcggcgtc ccacacatga ccgaagcgcg ggtggtcccc ttgcagaggc aagcgaacct
     5641 cgtcggaggg gattggtacg acgtcgacct catcggtcga acgctcacgg tcgtcgtcgg
     5701 agacgcgtcg ggcaagggca tagccgccgc gttaatggcg accgtggcct tgtccgctct
     5761 gagggccgag cgaggcaagg tggcggatcc caagcacgtc ctggaggcgg ccaaccgtgc
     5821 gttgcgggac gcgagcgatc cggagtcctt cacgaccgtg atctattcga cgctggacct
     5881 ggatacgggg gccacgagct ggatcaacat gggccatcat tcgccgttcg tgctgcgggc
     5941 ggccgagagc ccggatcatg cgacgcaggg ctactacctg gagggacccc ggaaccgtgc
     6001 actcggatgg ttcgaggacc ccggcctcac cgagaatcac gcccggttgg cccccggcga
     6061 ccgcctggtc ctcttcaccg acgggttcct ggaggccaaa tcgcaggagg gagaggtgtt
     6121 cggagagcat cgtctggcgg aggtcttgat caagcttgct ccgctcgacg gcgatgcact
     6181 tgagcacgag ctcgtggcgg aggtcgagcg cttcgcagcc ggtaagctcg atgacgacct
     6241 gacgatgatc atcatcgaat gggtagggac gcgtgaaggc gtacccgacc gagcctcagg
     6301 agaggaaccg tggcgcagca aaagatagat atcgacatca cgaatgaggg cgactaccgg
     6361 gtcttcaagc cgtccgggga cctcgacgtc tatacggtcg gttcgcttag ggatgcgatc
     6421 ggtgccgtga tcgaggagga gaacccgcgc atcgtcgtcg acctggatgc cgtgcccttc
     6481 atggattcgt ccggcctcgg tgccctgatg ggaggcgttc gtcggctccg tgaagccggc
     6541 ggtgatctcg cgatcgcttg cacccgcgag cagcacctca agctgttcac gatcaccggt
     6601 ttcggcgagg gcgtttcgat agctccgacg gtcgatgagg ccgccaaggg cttcaaggcc
     6661 taatcggcag agtccttctc gtcctcgtcg cgcgcccgcg cccttcggat agtcgccacc
     6721 agcagcatca cgaacacggc caccatggcc gaagtcactg aaatcaagat caattgcgct
     6781 gaggtcatgc gagactcttg atccggatgg atatctccca tttcgcgcaa gcgccgtccg
     6841 acgcctccgt cttcgaagat accttcagat tcgccgccta cacgatcgcc aggctgctcg
     6901 gcttctcggc tcacgcgatc ctttttggac tggttcccct cctgctattg gtgatccgtc
     6961 ctactttctc ggccttgcgc gggccggaat gggaccgagg ccgggagcgc gttgccgccc
     7021 ggctcgaagg attcgtgcaa gccgctctgg tggcggccgc gttgatcacc gctctaacgg
     7081 tcaccttgca aggagccctt atcgcagaag cgacgggagg gacgttctcg ctggattcgg
     7141 tcctcgacac cctcgagacg acgttcggac aatggcattt
//
LOCUS       DATMED010000378         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3758803, whole genome shotgun sequence.
ACCESSION   DATMED010000378 DATMED010000000
VERSION     DATMED010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758803"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VNP73_09840"
     CDS             complement(<1..1505)
                     /locus_tag="VNP73_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWL66260.1"
                     /translation="MTNHWVDFKNSDAFLVIGANPAENHPCGWKWAHVARDTRGAKII
                     HVDPRFTRTSAVADLWAPIRAGTDITFFNGLINYVLENELYHEEYVKLHTNASFIVSE
                     DYEFNDGLFAGYDEATRLYDPAKADYERIAGTELKPSITEKTPKAREGGTDGSALPAS
                     PAATPGFAKRDESLQDPRCVFQLMREHFSRYTPEMVSEVTGIPEEKFLEVAEIFGATG
                     TADKAGNVVYAVGLTQHTGGVQIIRGIGILQMLLGNVGKPGGGVNAERGHANIQGNTD
                     NAQSWEILPGYMAIPKPGMKTLDDYINTAALAQLDPNALNFFGSVYRAMTVSLLKQWF
                     GDAATKDNDFAFSWLPKPDKNWSWMTFHDEARAKRLHGLFNGGMSSVNIGPDSNRVIE
                     SLSNLKWFVVMDPFPTASSEFWQAPGVESEEVQTEVFFLPTTHWIEKAGAFVNSGRWA
                     QWKHQALPPEGDIRNDNYIIAQLFLRMRKLYEEEGGVFPDPILNLTWEYAD"
     gene            complement(1554..2150)
                     /locus_tag="VNP73_09845"
     CDS             complement(1554..2150)
                     /locus_tag="VNP73_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66261.1"
                     /translation="MELTRRDFFKVSAAGTAGAAWATFGFDLEPAYAQASELKISRAK
                     EIRSVCPYCGVGCSMIAYVAGHGETTSFNTKPYLVHIEGDPDSPINGGSLCPKGASTM
                     QLAVSEERVQAPMHREPGATEWSEISWDDALNRLARLMKDARDERFIETDDDGNLVNR
                     NEGFAWGGGATISNEEGYLTTKVFRALGAVGLEQQARV"
     gene            2328..>2882
                     /locus_tag="VNP73_09850"
     CDS             2328..>2882
                     /locus_tag="VNP73_09850"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWL66262.1"
                     /translation="MPQTSAITEKDVRSALGSVDDPELHRPITELDMVRGIVVVPGQV
                     RVSVALAVEDYIAKAELRDDVISALKALGAEQVFVDFTIMTDDQRTELRRRLHGDDAV
                     SMGDVFTSSRIIAVASGKGGVGKSSVSTNLAVALARRGQRTAIIDADVYGFSIPRMLG
                     IDRKPVIIDRMIVPPQAHGVSVISI"
BASE COUNT          505 a          917 c          901 g          559 t
ORIGIN      
        1 tggtccgcgt actcccaggt gagattgagt atcgggtcgg ggaagacgcc gccttcctcc
       61 tcgtagagct ttcgcatccg caagaagagc tgagcgatga tgtagttgtc gttcctgata
      121 tcgccctccg ggggaagggc ctggtgcttc cactgcgccc agcggcccga gttcacgaac
      181 gcacccgcct tctcgatcca gtgggtggtc ggcaagaaga acacctcggt ctgaacctct
      241 tccgactcaa cgccgggcgc ttgccagaac tccgaggacg cggtggggaa cgggtccatc
      301 acgacgaacc acttcaggtt cgacagcgac tcgatcaccc ggttcgaatc cggcccgatg
      361 ttgacgctgc tcatgccgcc gttgaaaagg ccgtggagcc tcttggcgcg cgcctcgtcg
      421 tggaacgtca tccacgacca gttcttgtcg ggcttgggca gccacgagaa cgcgaagtcg
      481 ttgtccttcg ttgcggcatc accgaaccac tgcttgagca ggctcacggt catggcgcgg
      541 tagacggaac cgaagaagtt aagggcgttc ggatcgagct gggccagtgc cgccgtgttt
      601 atgtaatcgt caagggtctt cataccgggc ttcggaatcg ccatgtagcc gggcaggatc
      661 tcccacgact gcgcgttatc ggtattgccc tggatgttcg cgtggccgcg ctcggcgttc
      721 acgccgcccc ccggctttcc gacattgccg agcagcatct gaaggatgcc gataccccgg
      781 attatctgaa ccccgccggt gtgctgggtc aggcccaccg cgtagacgac attgccggcc
      841 ttgtctgccg tgccggtggc accgaagatc tcggcgacct cgaggaactt ctcctcaggg
      901 atgccggtca cctcggacac catctccggt gtgtagcggg agaagtgctc cctcatgagc
      961 tggaacacgc agcgcggatc ctgcagcgac tcgtcgcgct tggcgaaccc cggtgtcgct
     1021 gcgggagacg caggaagagc actcccgtcc gtcccgccct ccctagcctt cggggtcttt
     1081 tccgtgatcg acggtttcag ttcggtgccc gcgatccgct cgtagtccgc cttcgcgggg
     1141 tcgtagagcc gcgtggcttc gtcgtaaccg gcgaagagac cgtcgttgaa ctcgtaatcc
     1201 tcggacacga tgaacgaggc gttggtgtgg agctttacgt attcctcgtg atacagctcg
     1261 ttttccagga catagttgat gaggccgttg aagaaggtga tatcggtacc cgcgcggatg
     1321 ggagcccata ggtcggcgac ggccgacgtg cgcgtgaagc gcgggtcgac gtggatgatc
     1381 tttgcaccgc gcgtgtcccg tgcgacgtgt gcccacttcc acccgcaagg atgattctca
     1441 gcagggttgg caccgatcac caggaacgcg tccgaattct tgaagtcgac ccaatgattg
     1501 gtcatggctc cccggccaaa cgaggcggcc aaactggcca ccgtagggcc gtgtcaaacc
     1561 cgggcctgtt gttctaagcc caccgccccg agggccctga agaccttggt cgtgagatag
     1621 ccctcttcgt tggagatggt cgcgcccccg ccccaggcga agccctcgtt gcggttgacg
     1681 aggttgccgt cgtcgtcggt ttcgatgaag cgttcgtcgc gcgcgtcctt catgagccgg
     1741 gcaagacggt tgagcgcgtc gtcccacgag atctccgacc actcggtcgc gcccggttcc
     1801 cggtgcatcg gcgcctgcac gcgctcctcg ctgaccgcga gttgcatggt gctcgcaccc
     1861 ttcgggcaca acgaacctcc gttgatcgga ctgtcggggt cgccttcgat gtggacgagg
     1921 tagggcttcg tgttgaacga agtggtctca ccgtgacccg cgacgtaggc gatcatcgag
     1981 cagccgacgc cacagtaggg acacaccgag cggatctcct tggcgcggct gatcttcagc
     2041 tcgctcgcct gcgcgtacgc cggctcgaga tcgaagccga aggtcgccca tgccgctccg
     2101 gccgtgccgg cggcggaaac cttgaagaag tcccgacgag tcagctccat gcgacccccg
     2161 atcctcgccc ccgcaagcct gtgagggcat gctctactgg tggactagtt agaccgtact
     2221 actcgtgcga caggcaggtc taggcaatgg gctacatcga gtagtccatt actgactagt
     2281 taacttggtt tgtctgagga gtcccggaca cggtcggagg gaggcccatg ccgcagacaa
     2341 gcgcaatcac cgaaaaagac gtgcgcagcg ccttgggctc ggtcgacgac cccgagctac
     2401 accgtcccat cacggagctc gacatggtca ggggcatcgt cgtcgttccg ggtcaggtcc
     2461 gcgtgtcggt cgccctcgca gtggaggact acatcgccaa ggccgagctt cgcgacgacg
     2521 tcatctcggc gttgaaagcg ctcggcgccg aacaggtgtt cgtcgatttc acgatcatga
     2581 ccgacgacca gcgcaccgag ctacgccgcc ggctccacgg tgacgacgcg gtctccatgg
     2641 gcgacgtatt cacatcctcg aggatcatcg cagtggcctc cggcaagggt ggggtgggca
     2701 agtcctcggt cagcaccaac ctcgcggttg ccctggcccg acgcgggcag cgcaccgcga
     2761 tcatcgacgc ggacgtctac gggttctcga tcccgcgcat gctcggcatc gatcgaaagc
     2821 ccgtgatcat cgatcgaatg atcgtgcctc cccaagcaca cggcgtgtcg gtgatctcaa
     2881 tc
//
LOCUS       DATMED010000379         4575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_684490, whole genome shotgun sequence.
ACCESSION   DATMED010000379 DATMED010000000
VERSION     DATMED010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684490"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VNP73_09855"
     CDS             <1..403
                     /locus_tag="VNP73_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWL66263.1"
                     /translation="GERRFDVPWEVLAAVNAVETRFGRILGPSSAGALGPMQFMPATW
                     EQYGAGGDIMDPHDSIIAAARYLSASGAPERMDDALFAYNRSNHYVDAILGYANQIMR
                     DPRYYYTYYFWEVFLRTTKGDVQYTGPGRDR"
     gene            complement(400..1437)
                     /locus_tag="VNP73_09860"
     CDS             complement(400..1437)
                     /locus_tag="VNP73_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWL66264.1"
                     /translation="MRLPLGLRRALAVLLSIAVVTPVAMTRAAPDQADLETARDRLFE
                     LEKDFQLVSEEYNLVREDLVAIQAEMAETRLVVRAIEGRMAVKQASAVSVATELYKAG
                     YAEMALESVLSAESLTEIEARLMYLKSTQTAQAKVFERLAVDQTLLEHNLDLLDVDRV
                     KAIAAEARLSDLREEIEGKVENQKDEVADLNAAIERARRRAAAAAAAAAVTEPGIPLE
                     PINIKPAPAPNERAQVAVDAALSQIGKPYQWGAAGPDSYDCSGLTMWAWAQAGVGLPH
                     NSGAQYSATVRVAREDLASGDLVFFGNPIHHVGMYIGNGQMVEAPYSGQRVRVVSMNR
                     SDYVGAGRPGV"
     gene            complement(1448..2251)
                     /locus_tag="VNP73_09865"
     CDS             complement(1448..2251)
                     /locus_tag="VNP73_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWL66265.1"
                     /translation="MEAAFFDLDKTIVARSSPLALGRSFMREGLIGRSFLLKSIYAQL
                     IFQLMGADEEKMERMREEAAKLTAGWEADNVRRVVNEVLEEVISPLIYAEALELIHDH
                     RMAGRLICIVSSSPEEIVEPMAGMLDVDRWIATKPAIDDEGKYTGELDFYAYGPHKVT
                     AIKELAEELGIDLEGSYAYSDSETDLPMLESVGHPVVVNPDKGLRRLAAERGWKVETF
                     KNPVTLRDRLPQLRAPEMTLVGGAKALAGVAGVAAAAWWISRRAGARKG"
     gene            2383..3210
                     /locus_tag="VNP73_09870"
     CDS             2383..3210
                     /locus_tag="VNP73_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66266.1"
                     /translation="MAAARAFDAAPADWQVRLFEDVPEGAHAVVVCPDVDSHGILFDP
                     SQPAAAFEEVEARLACGHEGRSIHVAGTGGGVGTTSVAIHLAAALGRTSSTCLIELDP
                     NRAMADRLGLDPSDARWEEIDDGSDGLGRAAVPIGGFRVLTRPEVVADGTCKCVLEAA
                     INTFALTIIDAGAFSPSCVHQPSLLVMPPSLPGARRARAFLDERPDEVWGVVSNRLGP
                     GSETTRAMIERNLGRRLLSELPCTPGLRDAEDEGRILSTPWSRWLQKITRLAALVSG"
     gene            3210..4235
                     /locus_tag="VNP73_09875"
     CDS             3210..4235
                     /locus_tag="VNP73_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWL66267.1"
                     /translation="MMDLTEALYENPAIGTLDPGARRLALRELAISRGVSDLDVATRL
                     ADRIDGYGPLAPLVDDRSITDILINGPDGVWIERDGSLEQTPIRFDDVDHLTGFVQRM
                     LGDQGVAVDALHPIADARMRDGSRVHAVLPPAAPDGPLVSIRRFPRAVWTLEDLAKRG
                     ALMAVEVQQLADLVRRRTTVAISGGTGSGKTTLLNALLSRVDGGERVVGIEETRELRP
                     RCEHFVSLLSRPDNMEGRGGISPAELMRAALRMRPDRIVVGEVRGAEALVALQAMSTG
                     HPGSMVTLHANSPGDARRRMTSMALQASSGLSEGALQDLVERSFGAVIQLARVGGTRR
                     VVSIEET"
     gene            4210..>4575
                     /locus_tag="VNP73_09880"
     CDS             4210..>4575
                     /locus_tag="VNP73_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWL66268.1"
                     /translation="MWFRSKRPEPSIADALDVLAGLLRAGTSIRAALTGWHVEAPSSF
                     TGSLRELDRLLTLGMSPVDSLSGLAKDERFRAVAHELAGSLSVAERVGADASAAVARI
                     AAEVRDRAADTAAAVAAASG"
BASE COUNT          739 a         1439 c         1503 g          894 t
ORIGIN      
        1 gggcgagagg cgcttcgacg ttccgtggga ggtgctcgcg gccgtcaacg cggtcgagac
       61 gcgtttcggc cgcatcctgg gaccgagttc tgccggagcc ttgggaccga tgcaattcat
      121 gcccgcgaca tgggagcagt acggagccgg tggagacatc atggatccgc acgactcgat
      181 catcgccgcc gccaggtatc tgtcggcatc cggtgccccc gagcgcatgg acgacgcgct
      241 gttcgcttac aaccgcagca accactacgt ggatgcgatc ctgggctacg ccaaccagat
      301 catgagggat ccgcgctact actacaccta ttacttctgg gaggttttcc tgcggacgac
      361 caagggcgac gtgcagtaca ccggtccggg ccgggatcgt taaacgccgg gacgcccggc
      421 ccccacgtaa tccgaccggt tcatcgatac gacgcgcacc cgctggccgg agtaaggggc
      481 ctcgaccatc tgtccgttcc cgatgtacat cccgacgtga tggatcgggt ttccgaagaa
      541 cacgaggtca ccggaagcga gatcctctct tgcgacgcgg accgtggccg aatattgggc
      601 gcccgagttg tggggtaggc cgacaccggc ctgtgcccat gcccacatcg tcagccccga
      661 gcagtcgtag ctatcgggtc cggccgcgcc ccactggtaa ggcttcccga tctgagacag
      721 ggccgcgtct acggcaacct gcgcgcgctc gttcggtgcg ggcgcgggct tgatgttgat
      781 cggctcgagg gggatgcccg gctctgtcac cgctgcagca gccgcggccg ccgccgctcg
      841 acggcgcgca cgttcgatcg cagcattgag gtcggctacc tcgtctttct ggttttcgac
      901 cttgccttcg atctcttcgc gcaggtcgct cagacgcgct tcggccgcta tggccttcac
      961 gcggtcgacg tcgagcaggt cgaggttgtg ctcgagcaag gtctggtcta cggcgagccg
     1021 ttcgaagacc ttggcctggg ccgtctgcgt cgatttgagg tacatgaggc gcgcttcgat
     1081 ctccgtcagc gactcggcgg acagcaccga ctcgagggcc atctctgcgt aaccggcttt
     1141 gtagagctcg gtcgcgactg atacggccga cgcctgcttg accgccatcc ggccctcgat
     1201 cgccctgacc acgaggcgcg tctcggccat ctcggcctgg atcgcgacga ggtcctcgcg
     1261 cacgaggttg tactcctcgg acacgagctg gaaatccttc tcgagctcga acaagcggtc
     1321 cctcgcggtc tccagatcgg cttgatcggg ggccgcgcgc gtcatggcca ccggggtgac
     1381 cacggcaatg ctcaatagaa cggcgagtgc ccgccgcagg cctaggggaa gacgcacggc
     1441 ctcgacctta gccttttcgc gcccccgctc ttcgacttat ccaccatgcg gccgctgcga
     1501 cccccgctac cccggccagc gctttggccc ccccgaccag ggtcatctcg ggggccctca
     1561 gctggggtag ccggtcacgg agcgtcaccg ggttcttgaa cgtttcgacc ttccagcccc
     1621 gttccgccgc caaccggcgc aggcccttgt cggggttgac gacgacggga tgaccgacgc
     1681 tttcgagcat cggtaggtcg gtttcggagt ccgagtaggc gtaggaaccc tccaggtcga
     1741 tacctagctc ctcggctagt tccttgatgg cggtcacctt gtgggggccg tacgcgtaga
     1801 agtcgagctc gcccgtgtac ttgccctcgt cgtcgatggc gggcttcgta gcgatccagc
     1861 gatcgacatc gagcatgccg gccatcggct cgactatctc ttcaggtgag gacgacacga
     1921 tgcagatcag gcgacccgcc atccggtggt cgtggatcag ctcgagtgct tccgcataga
     1981 tcaacggtga gatgacttcc tcgagcacct cgttcacgac tcgacgcacg ttgtcggcct
     2041 cccaccccgc ggtgagcttt gcggcctcct cgcgcatccg ctccatcttt tcttcgtcag
     2101 cacccatcaa ttgaaagatc aattgcgcgt agatgctctt gagaaggaaa gagcgaccga
     2161 tgaggccttc gcgcatgaac gagcggccga gcgcgagtgg actcgagcgg gcgacgatgg
     2221 tcttgtcgag gtcgaagaag gccgcttcca tatctcgatt gtgcctcctc agtgcaacgc
     2281 gccggtttgt gcgcgatgtc acggctcacc aacaggttcc tgcgtcaatg ttgggtggtg
     2341 cgagtccgag tagccatcgt tggccgtgat cccgatctca ggatggcagc agcccgggcc
     2401 tttgacgcgg cgcccgccga ttggcaggtg cgtctgttcg aggacgtacc cgagggagca
     2461 catgccgtcg tcgtgtgtcc cgatgtcgat tcgcacggga tcttgttcga cccttcacag
     2521 ccggccgcgg ccttcgaaga ggtagaggca cggttggcat gtggccatga aggcaggtcg
     2581 atccatgtcg ccggcaccgg tggcggcgtg ggaacgacga gcgttgcgat ccatctcgct
     2641 gcggctctag gccgcacctc ttcgacctgc ctgatcgagc tcgatccgaa ccgggcgatg
     2701 gccgatcggc ttgggctcga tccgtccgac gcccgatggg aggagatcga cgatggatcc
     2761 gatggtcttg gacgtgcggc tgtgcccatt ggtgggttcc gcgtgctgac gcgacctgag
     2821 gttgtggccg acggtacatg caagtgtgtc cttgaggcgg ccatcaacac tttcgcgctg
     2881 acgatcatcg atgcaggcgc cttttcaccc tcctgtgtcc accaaccttc tttgctggtc
     2941 atgcctccct cgcttcccgg tgcccgtcgc gcacgggcgt tcctcgatga acgacccgac
     3001 gaagtctggg gtgtcgtctc caacaggttg ggtccgggca gcgaaacgac gcgcgcgatg
     3061 atcgagcgaa acctgggacg ccgtctcttg tctgagcttc cctgcacacc gggattgcgt
     3121 gatgcggaag atgagggccg gatcctgtct acgccgtggt cgcggtggct ccagaagatc
     3181 acgcgattgg cggcgctcgt ctcgggatga tgatggatct aaccgaagcc ctgtacgaga
     3241 atccagcgat cggcacgctc gatcccggcg cccgcaggct ggccctgcga gagctcgcga
     3301 tctctcgcgg tgtctcggat ctcgatgtcg ccacgcgtct cgccgatcga atcgacgggt
     3361 acggccctct tgcaccactc gtcgacgacc gatcgatcac ggacatcctt atcaacggtc
     3421 cggatggggt ttggatcgaa cgggatggca gcctcgaaca aaccccgatc aggttcgacg
     3481 atgtcgacca cctgacggga ttcgttcaac ggatgctcgg ggaccaaggt gtggcggttg
     3541 atgcgttgca tccgatcgcg gacgctcgca tgcgagacgg ttcccgggtt cacgcagttc
     3601 tcccaccggc ggctcccgac ggtccgctcg tttcgatccg gcggttcccg cgtgcggtgt
     3661 ggacgctcga agacctggcc aaaagaggcg ccctcatggc agtcgaagtg cagcagcttg
     3721 ccgatctggt ccggcgccgc acgaccgttg ccatcagcgg tgggacggga tccggtaaga
     3781 cgacgcttct caatgcgttg ttgtcccggg tggacggcgg cgagcgggtc gtcgggatcg
     3841 aagagacccg cgagcttcgt cctcggtgcg aacacttcgt ctcgctcctg tcgagacccg
     3901 acaacatgga aggacggggt gggatctcgc ccgccgaatt gatgcgggct gctttgcgca
     3961 tgcgtccgga tcggatcgtc gtcggcgagg ttaggggggc cgaggcccta gtcgcgctgc
     4021 aggcgatgtc cacaggacat ccgggatcga tggtgaccct gcacgcgaac tcgccaggcg
     4081 acgcgcgtcg caggatgaca tcgatggcgc ttcaagcttc gtcgggcctc tccgaagggg
     4141 cgctccagga tctcgtcgag aggtcgttcg gggctgtgat ccaactggct cgtgttggag
     4201 gaacgagacg tgtggtttcg atcgaagaga cctgagccct cgatcgcgga cgcgctcgat
     4261 gtgctcgcgg ggctactacg ggccggaaca tcgataaggg cagcgttgac cgggtggcat
     4321 gtcgaggcgc catcttcctt taccgggtcc ctgcgcgagt tggatcgtct actgacgctc
     4381 gggatgtccc cagtggactc attgtccggg ctggccaagg acgagcgctt tcgagccgta
     4441 gctcacgagt tggccggctc gttgagtgtg gcggagcgag ttggtgcaga cgcgtcggcc
     4501 gcggtggccc gtatcgctgc ggaggtgcgt gatagggctg cagatacggc ggcagcggtc
     4561 gccgctgcgt cggga
//
LOCUS       DATMED010000380         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2621605, whole genome shotgun sequence.
ACCESSION   DATMED010000380 DATMED010000000
VERSION     DATMED010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2621605"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1642)
                     /locus_tag="VNP73_09885"
     CDS             complement(32..1642)
                     /locus_tag="VNP73_09885"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HWL66269.1"
                     /translation="MPEGHGGLAATHALKALGVDHLFTLSGGHIFPIFDGCKQDGIRI
                     VDVRHEQNAAFAAEGFARLTRSLGVTAITAGPGVTNAISPLAQARFNGAPVLALAGRA
                     PAFRWGQGSLQEIDHVPFVDPLAPAKTVTDTSQIPAEVIAAAVAALTPPRGPRFLDFP
                     LDVLFNSTSFEVPEVNMQRPGADSASIESIAAALSGAERPVLVAGSNVWLDRAENALV
                     ALAESAEVPVFTNGQGRGCIPADHRLAFSRARSTAFKGADLVIVIGTPMDFRLGFGQF
                     GAGAPVIHIETAPELLAAHVDLKASAAGPIDQILTGVADALEGPADTKEWVESLRAAE
                     EAKRDAAQAELHSSATPIHPMRIYGDLVPLLDRDAVVIGDGGDYVSYAGREVPSYEGG
                     CWLDPGPFGCLGIGPGYAMAARLKYPERQIVLLYGDGAVGFSAGDLESLVRHKLPVVC
                     VVGNNGIWGLEKHPMRALYGYDVAAELRPGIRYDQTMESFGGRGELVEDPDEIGPALK
                     RAFDSGEPYLVNVLTDPEIAYPRSANLA"
     gene            complement(1655..2626)
                     /locus_tag="VNP73_09890"
     CDS             complement(1655..2626)
                     /locus_tag="VNP73_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HWL66270.1"
                     /translation="MVPAVRPVRGEVALIGHEQVLGAIEPRQAIDRVRDSFVRFARGE
                     WTMPPKTYVQSPPNGDFRAMPALGSGFAILKWVTSFPGNPGRGLPTVTGIVLVSDADN
                     GEPLALVDARAVTALRTGASAAVATELLARPGANSVGIVGCGLHGAWAARCLAELGFS
                     EGICFDLDGDAAKGLADELGWRGGSLEDALACDVVTTVTPGKQPVIERGHIRSGMHIN
                     ALGADGSGKAEMDVSAVAACRVFCDEWEQASHGGEITGAVQQGLLDRSSVTEIGSVLN
                     GASGGRESEEDATLFDSTGLAIQDLAIVVALMDSLASGDLQPETVSL"
     gene            2734..>3016
                     /locus_tag="VNP73_09895"
     CDS             2734..>3016
                     /locus_tag="VNP73_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66271.1"
                     /translation="MSDPAPPSEAPEETSEEKESWHFDEGDEIVPGRHALKKLGGGYD
                     YEAYLSWDDRLFSLVVAKCLRPHLVDDKHSLGSIAREAEHLLKLNHPVVV"
BASE COUNT          569 a         1023 c          941 g          483 t
ORIGIN      
        1 ttgcggagac tacggggccg ggacggcgct gctaggccag gtttgcagac ctcgggtaag
       61 cgatctcggg atcggtcagc acgttcacca gatacggctc gccggagtcg aacgcccgct
      121 tcagcgcagg accgatctcg tcgggatcct cgaccagctc gccgcggccc ccgaacgact
      181 ccatcgtctg gtcgtaacgg atccccgggc gtagttccgc tgcgacgtcg tacccgtaga
      241 gagcgcgcat cggatgcttc tccagccccc agatgccgtt gttaccgacg acgcatacga
      301 ccggaagctt gtgacgaacg agactctcca gatcccccgc agagaagccg accgcgccgt
      361 cgccgtacag cagcacgatc tgtcgttccg gatacttcag ccgcgccgcc atcgcgtaac
      421 ccggaccgat gcccagacaa ccgaacggtc ccggatcgag ccagcaaccg ccttcgtagg
      481 acggaacctc tcgtcccgcg taggagacgt agtcgcctcc gtcgccgatg acgaccgcat
      541 cgcggtcgag caatggcacg aggtcgccgt agatgcgcat gggatggatc ggggtcgcgc
      601 tcgagtggag ttcggcctgg gccgcgtcac gcttcgcttc ctcggccgcc cgtaacgact
      661 caacccattc cttcgtgtcg gccggtccct ccagcgcgtc ggcgacgcct gtcaggatct
      721 gatcgatcgg tccggcagcg ctcgccttca gatcgacgtg agcagcaagc agttcgggtg
      781 cggtttcgat gtggatcact ggggcgccgg ccccgaactg gccgaaaccc aacctgaagt
      841 ccatcggagt gccgatcacg atgacgaggt cggccccctt gaacgcggtc gagcgagcgc
      901 gcgagaaggc gagtcggtga tcagccggga tacaacctcg gccctgaccg ttggtgaaca
      961 cgggaacctc ggcactctcc gcgagcgcga ccaacgcgtt ctccgcccgg tcgagccaca
     1021 cgttggatcc cgccaccagc accggtcgct cagcaccgct caacgcggcc gctatcgact
     1081 cgatcgacgc agagtccgca ccgggccgct gcatgttcac ctcgggtact tcgaacgaag
     1141 tcgagttgaa gagaacatcc agcgggaaat cgagaaaacg cggtccacga ggtggagtca
     1201 gagctgcgac cgcggccgcg atgacctcgg ccgggatctg cgaggtgtcg gtgacggtct
     1261 tcgccggcgc gagcggatcg acgaacggaa cgtgatcgat ctcctgcagc gagccctggc
     1321 cccatcggaa tgcaggggcc ctccctgcga gtgcgagaac gggggcaccg ttgaagcgag
     1381 cctgcgccag agggcttatc gcattcgtaa cgccgggacc ggccgttatc gccgtcaccc
     1441 cgagcgagcg cgttagacgt gcaaaccctt cggcggcgaa tgcggcgttc tgctcgtggc
     1501 ggacgtcgac gatccggatc ccgtcttgct tacaaccgtc gaagatcgga aagatgtggc
     1561 cgcccgacaa ggtgaagagg tgatcgacgc ccagcgcctt gagtgcgtgg gttgcggcga
     1621 gaccaccgtg tccttcaggc atcgtctcgg cagcttaaag ggagacggtc tctggttgca
     1681 gatcgcccga cgcgagagag tccatcagcg ccacgactat cgcaagatcc tgaatggcca
     1741 gaccggtgga atcgaacagg gtcgcatctt cctcggactc ccggcctccc gaagcgccgt
     1801 ttagaacgga cccgatctcg gtgaccgacg agcggtcgag aagcccctgt tgcaccgctc
     1861 cggtgatctc gccgccgtga ctcgcctgct cccactcgtc gcagaagacg cggcatgcag
     1921 ccaccgccga aacgtccatc tcggccttgc ccgaaccatc cgcgccgagc gcgttgatgt
     1981 gcatcccgga acggatgtga cctcgctcga tcaccggttg cttccccggc gtaacggtcg
     2041 tgaccacatc gcaggcaaga gcatcctcga gcgacccgcc ccgccagccg agctcgtcgg
     2101 ccaagccctt cgccgcatcg ccgtcgaggt cgaagcagat cccttcgctg aagcccagct
     2161 ccgcgagaca ccgtgccgcc catgcaccgt gcagtccaca cccgacgatc ccaaccgagt
     2221 tcgctccggg gcgggcgagc aactcggtgg caactgcggc ggaggcgccg gttcgaagcg
     2281 cggtaacggc gcgcgcatcc acgagcgcaa gtggttcacc gttgtcggcg tcactcacca
     2341 acacgatccc ggtcacggtc ggcaggccgc gtccggggtt accggggaac gacgtcaccc
     2401 atttcaggat cgcgaacccc gagccgaggg ccggcatcgc tcgaaagtca ccgttaggag
     2461 ggctctgcac gtacgtctta gggggcatcg tccactcacc gcgagcgaac cgaacgaaac
     2521 tgtcccgcac ccgatcgatc gcctgccgag gctcgatcgc gccgagcact tgctcgtgac
     2581 ctatgagggc cacctcgcct ctgaccggtc gaacggcggg aaccatcgcc agaggctaaa
     2641 tcgataacaa tggctcataa gcgatcgtct cgacgatcgc gccatccgtg cgaagggctg
     2701 cccgcgtagc gggcatgcct gagggaggac aaaatgagcg atcccgcgcc cccgtccgag
     2761 gctcccgaag aaacctccga agaaaaggag tcctggcact tcgacgaggg agacgagata
     2821 gtccccggcc gtcacgctct gaagaagctc ggcggcggtt acgactacga ggcatacctc
     2881 tcgtgggacg accgcctctt ctcactggtc gtcgccaagt gtctgcggcc gcatctggtc
     2941 gacgacaaac attcgttggg gtcgatcgcc cgcgaagccg agcacctgtt gaagttgaac
     3001 catcccgtcg tggtcc
//
LOCUS       DATMED010000381         3332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1841048, whole genome shotgun sequence.
ACCESSION   DATMED010000381 DATMED010000000
VERSION     DATMED010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841048"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VNP73_09900"
     CDS             <1..424
                     /locus_tag="VNP73_09900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007321125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL66272.1"
                     /translation="GRKSAVTKAIEHYGLGDSVRFETGIDKLRLVELYAEAEIAVVPS
                     LYEGFSLPAIEAMSCRVPVIATTAGALPEVIGGAGIMVPPSDAGALASAIMRLLENKS
                     RRVELGSDGRRRVLERFTWRAAAEKTTAVYERAISDHG"
     gene            411..1151
                     /locus_tag="VNP73_09905"
     CDS             411..1151
                     /locus_tag="VNP73_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66273.1"
                     /translation="MITVDYERLELGSGDLLLDVGSGNGRHTFEALKRGANIVAVDLD
                     EMYLPIVREMALAMRDQGEARPHATIAAVQGTALSLPFPDSTFDRVIAAEVLEHIPED
                     VTAMRELARVLKPGGVAAISVPRAWPERICWALSKEYTTSSGGHVRIYRRDELITRLE
                     GAGLRFLAHHHAHAFHSAYWWVKCAVGVDRDEALAARTFKRFLEWQIVKRPAGLDSLE
                     RALDPILGKSLVVYVRKPASVSEARRVA"
     gene            1141..2157
                     /locus_tag="VNP73_09910"
     CDS             1141..2157
                     /locus_tag="VNP73_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HWL66274.1"
                     /translation="MLLDLAATAEAIAGIQAEDGSIPHWPGHHADPWNHTEALMGLDI
                     VGMHVEARRGYRWLAAVQRPDGAWASGYMGEVITDPTMDANFCAYPATGIWHHYLITG
                     DDAFVDEMWPMVERAIEFVLGLQTTSGHILWARDERYQPWTGPLLTSCSSIYLSLRCA
                     IALSETVGLDKPEWELALTCLRDAIVDEPDAFDDKQLFSMDWYYPVLTGVIEGDQARD
                     RIFERWDTFVIDGHGCRCVSNRPWVTTGETAELILALDACGLHEESHLMFDWLHHLRA
                     EDGAYWMGATYPDQTIWPRQKPTWGSGSVVLAADALGRSTPASGLFRGETFERVLGQV
                     TDPL"
     gene            complement(2101..2715)
                     /locus_tag="VNP73_09915"
     CDS             complement(2101..2715)
                     /locus_tag="VNP73_09915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017568112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66275.1"
                     /translation="MPEEEGLALHRVGLRGAEVGPLVEIGSYCGKSAVYLGAAARERN
                     VVLYSIDHHRGSEEQQPGEEFHDPRLVDPVTGKVDTFPFFRKTIQSADLDDTVIPIVG
                     ESSRVAAGWTTPLGLVFIDGGHGEEAARADLVGWAPHITAGGLLAIHDVFEDPAEGGR
                     PPYLIYRAATATGAFKEIGQSGSLRVLERIGDLPENPFERLASE"
     gene            3006..3230
                     /locus_tag="VNP73_09920"
     CDS             3006..3230
                     /locus_tag="VNP73_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66276.1"
                     /translation="MTALRREWLAAGVAFSLAGVVLVLVSIVAQATLDNVATGPERPF
                     GVLLVVVGIVFMTRARHRPGADGLSMRDPE"
BASE COUNT          588 a         1067 c         1065 g          612 t
ORIGIN      
        1 cggccgcaag agtgcggtca ccaaagccat cgagcactat ggactgggcg attccgttcg
       61 cttcgagact gggatcgaca agctgcgcct agtcgagctt tatgcggaag cagagatcgc
      121 cgtggtcccc tctctttacg agggcttctc gctacccgct atcgaggcga tgtcgtgtcg
      181 cgtcccggtc atcgctacta ccgcgggcgc gctgccggaa gtgatcggcg gcgcaggcat
      241 catggttccc cccagcgacg ccggtgctct ggcgagcgcg atcatgcgcc tcctcgagaa
      301 caagagtcgc cgggtcgaac tcggatcgga cggacgacgg cgcgtcctcg agaggttcac
      361 gtggcgggcc gccgccgaga aaacgaccgc cgtctacgag agggccatca gtgatcacgg
      421 ttgactacga gcgtctcgag ctcggctcgg gagatctctt gctcgacgtg ggctcgggaa
      481 atggacggca cacgttcgaa gcattgaaaa gaggcgccaa catcgttgcc gtcgacctgg
      541 acgagatgta cctaccgatc gttcgcgaga tggcgctggc gatgcgggac cagggcgagg
      601 cacgcccgca cgcgaccatc gcggccgtac agggaaccgc gctgagcctg ccattcccgg
      661 attcgacgtt cgatcgtgtg atcgcagcgg aggttctcga gcacatcccc gaggacgtga
      721 ccgccatgcg tgagctggct cgggtattga aacccggcgg ggttgcagcc atatcggtcc
      781 cccgagcgtg gcccgaacgg atctgctggg cgttatccaa ggaatacacc acgtcctccg
      841 gagggcacgt ccggatctac agacgcgacg aactcatcac gcggttggag ggagcgggct
      901 tgcggttcct cgctcaccat cacgctcacg cgttccactc ggcgtactgg tgggtcaaat
      961 gcgcagtggg ggtcgatcgt gacgaagccc tcgcagcccg aacgttcaaa cgattcctcg
     1021 agtggcaaat cgtcaagcgc cccgccggac tcgactccct cgagcgggcg ctcgatccga
     1081 tcctcggtaa gagcctggtc gtctacgtcc gcaagcccgc atcggtttcg gaggcccgcc
     1141 gtgttgcttg acctcgccgc taccgcggag gcgatcgccg gcatccaagc cgaagacggc
     1201 tcgatcccgc actggccggg gcaccacgcc gacccgtgga accacaccga agccctgatg
     1261 ggtttggaca tcgtcgggat gcatgtcgag gcgcggcgtg gatacaggtg gctggcggcg
     1321 gtgcaacgcc ccgacggtgc ctgggcgtcg ggctacatgg gcgaggtgat caccgatccc
     1381 acgatggacg cgaacttctg cgcgtacccc gcgaccggga tctggcacca ctacttgatc
     1441 accggcgacg acgccttcgt cgacgagatg tggccgatgg tcgagcgagc gatcgagttc
     1501 gttctgggtc ttcagaccac gagcggacac atcctgtggg cgcgcgacga gcgttaccaa
     1561 ccgtggacgg ggccccttct gacgtcgtgt tcgtctattt atctcagcct gcgttgcgcg
     1621 atcgcgttat ccgagaccgt tggcctggac aagcccgaat gggaactcgc tcttacgtgc
     1681 ctgcgcgatg cgatcgtcga cgagcccgac gcgttcgacg acaagcaact tttctcgatg
     1741 gattggtatt acccggttct caccggtgtg atcgaaggtg accaagcacg ggatcggatc
     1801 ttcgaacgat gggacacgtt cgtcatcgac gggcatgggt gcaggtgcgt atcaaaccgg
     1861 ccctgggtga caaccggcga gacggccgag ttgatcctgg cgctcgacgc gtgcgggttg
     1921 catgaggaat ctcacctgat gttcgactgg ttgcatcatc tgcgcgccga ggacggggcc
     1981 tactggatgg gagcgacgta cccggaccag acgatctggc cccgtcagaa gccgacgtgg
     2041 ggctcgggct cggtcgtcct ggctgcagac gcgctggggc gttcgacgcc cgcgtcgggg
     2101 ctattccgag gcgagacgtt cgaaagggtt ctcgggcaag tcaccgatcc tctctaggac
     2161 ccgcaagctc ccgctctggc cgatctcctt gaacgccccc gtcgccgtcg cggcgcggta
     2221 gatcaggtac ggcggtcggc caccttcagc cgggtcctcg aagacgtcgt ggatagcaag
     2281 gagccctccg gcggtgatgt gaggagccca cccgaccaga tcggcccgtg cggcctcttc
     2341 gccatggcca ccatcgatga acacgagtcc aagcggggtt gtccagccag ccgccacgcg
     2401 gctcgattca ccgacgatcg ggatgacggt gtcgtcgagg tccgcactct ggatcgtttt
     2461 ccggaagaag ggaaaagtgt ccaccttgcc ggtgacgggg tccaccaagc gcgggtcgtg
     2521 aaactcttca ccgggttgct gttcctcact cccgcggtgg tgatcgatcg agtagaggac
     2581 cacgttccgc tcacgcgccg ccgctcccag gtacacggcc gatttcccgc agtacgagcc
     2641 gatctccacc aacgggccga cctcggcccc ccgaagcccc actcggtgca gcgcgagccc
     2701 ctcttcttcg ggcatgaacc cgatggcata tcgagcagca gcgcgcaggc gctcgtccac
     2761 gccgcaacta tgtcatcgca gagcgcggac tggaaaagaa acattcgtgg gatcccgtcg
     2821 gcttgtcatc ctcgacgcct cattgatgat cggggccgtt tgcatcgggc tcctcgccct
     2881 ctcgatcggt ctgatcggcg ttttctactt cggtggggct ctcttcgtct ccaacgcaac
     2941 gccttaccag cgccttgcgg gcatctcaac cttttatccc gaccgtcgcc ggcatcgtcg
     3001 gaatgatgac ggccctaagg cgcgaatggc tcgcagcggg ggtcgctttc tccctggccg
     3061 gggtcgtact ggtcctcgtc tcgatcgtcg cccaagccac actcgacaac gtagccaccg
     3121 gccctgaacg gcccttcggg gtcctcctgg tcgtagttgg gatcgtattc atgactcgag
     3181 cgcgacaccg tccgggggcg gatggcctgt ccatgcgcga tcctgagtaa tctcgtcctc
     3241 gtttcgaaga cctatccgag gagaacgacg tggggggaag catcgacaag atcagggacg
     3301 cgatcctgaa cgacgcatca ggcgaagaca tc
//
LOCUS       DATMED010000382         8418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2998875, whole genome shotgun sequence.
ACCESSION   DATMED010000382 DATMED010000000
VERSION     DATMED010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998875"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1129
                     /locus_tag="VNP73_09925"
     CDS             59..1129
                     /locus_tag="VNP73_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWL66277.1"
                     /translation="MGPSGTPARATDDRELVLAFQAGERAAYDEMYRRYHGRVSAICS
                     RILNNSQDAEEAAQETFLRAYQALPRFNGNFLLGAWLARIAANASVDQLRTKSRSPLV
                     GLPSDHEDPGDDVDPEDVVVGEHPRLDIALAEIKPLHARALAWRNVDGLSHLEIAELL
                     SMTPSQVKALLHRARVSLRKAWDRAEGWAIAPLFGIRSLWPERHGNSGSFWNAAPSLS
                     PLLAEKVAATALVVAVALSGLPTNASDTSTPALTSPRLGIGATSHGSIASAEVRPSLT
                     TSEVSAPNEPASGSDAVADLTEKIEEIKGTLNGREKLDEDPPRPEEPGGKDPSTEAAS
                     TARKVVKKVKDLLPHLSVDTSL"
     gene            1169..1990
                     /locus_tag="VNP73_09930"
     CDS             1169..1990
                     /locus_tag="VNP73_09930"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWL66278.1"
                     /translation="MPDTLVPRPPEPPPSFLEGDRFSVLCEIEPPRQPDMSHVHEQVE
                     ALKPAVDAFLIPDNHLGRATVSSVSVAHEVAYMGGRSIACMNARDRNLLGFRRDLLTA
                     ASYGVDHFLFVYGDAPREGRRTTDLTVRGMIDEVRGFGSGPLFQGYPDFRIGTVTKTS
                     RPLAWRQRADYVLVQITFSFERLMRWRESIDFDGRIYPAVLVLASSRMARRINATLRD
                     VRVPDHIIDKLDDGKHVGLDLACEQIEAIKDSGAFDGVHLVPVGRYREMAERLQT"
     gene            complement(2042..4003)
                     /locus_tag="VNP73_09935"
     CDS             complement(2042..4003)
                     /locus_tag="VNP73_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66279.1"
                     /translation="MRSLGHAVAAGVLLFAVLPATEALAAPTVEFLNPSGYSPTIRIS
                     DKEDADDTYHLNVWVGEVPTDALVEFELSNAAGTEATVVGTRAGSDAWEGELSLEGVA
                     DGQYTLQAILYENFAGVGTGNEVDRSEPMSVLVQAADIPPGSEAETVEITHPENGGQL
                     GFYAAPGKAASAVIEGTTSEGASQVRALYSVSNVGGAPEWLACGSAVPSEGDVSVKCT
                     LQDDGPAARVKAVALVANSTPSPGMPVPPADETGDAHRVVVYGATPTSLRMDPDSEEV
                     DEGTCFAVTATALDQAGNVVSGVNIDIHAQGPDDQLKFANQRGTSDAFQAPDKSHSGT
                     EPTVKCAETDAEGSQGDHNVPGAADQKHIESTTGTGADGSFTFVLRSSVEGGTLITAW
                     SDDNDDDLQGATEASGGAQLGWGTDPPQAPKTLILDPSSTTAATGDCVKMTAVARQGG
                     NALDARDIDVHALGPEGVSFCTPEGGSNAFPPDQGEHAGGYHGDGTGHIEGMTDANGS
                     FVFGVTSARQGTTDLLVWLDNDGDDVFDADDEDEISANGQIGWGGQTKNEPTQVSIRY
                     KRSAFRGAARSAASGCRSERTIDLRKQKRGRDPKVGTAVTNANGNYRIREPRAKGKYY
                     ARAAQKTFIDENGDNIVCEAGKSRTKRVR"
     gene            complement(4009..7464)
                     /locus_tag="VNP73_09940"
     CDS             complement(4009..7464)
                     /locus_tag="VNP73_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL66280.1"
                     /translation="MSFFRQPLSLSGDRLLARGALAVIGTAVFLVLVLTSPSRGAGPV
                     ILAQPSGSQTSGSPSGSPSGSPSGGPSGSPAPTGTTIALLNPAEGYDPFTAPPASAAS
                     EHIPQISKVMDADETYHVTASTSGAPAGATATAVYAADGGGETTIGALEQVEDSPGVW
                     EAHWDVPESLDGTTGRVIARLFSGGAKIAESATPAEIDLAEETVEITWPPNGGGLGFF
                     KSTAGPWRFVLDGVASANARAYVFFSTTPKGQIPEFTGDCGFVDPTGAFQVTCTLPPT
                     ADPATVTSVAMVAAEPDTPTGQGFFTQDSGDVHAVVPYQQDPSKMTASISSVEPTEPD
                     ASYPAGARREANTGCLAFDMTVLDQFQRPVQGANVDVHVVGPVDEAGSAVGGDSSARK
                     PPDLGSHVVAPAWDCDSPGGHIGQEGKHEKPEELDVKHFEAMAGTGLGEDPPGQFRFH
                     VFSPNAGDMQITAWVDEENNPATVDALRDLNGDTREKVDAFAIARAQWLEKPVALAIS
                     PSGSAGLIGSCTPFTVKTRAGKSVLDGVNVDVHASGPSNDLDFCTPGGGSVLQAPDAT
                     PSPATHTSEAGESDEAQHPGATPTDPTIQHTEGTTNEEGDLIIGLMSPVSGDTTLTAW
                     VDGEPGADDDELAATEPTAAASMSWAASAQDAKVRFVNPSGYGGAGDAVSNLQDADPV
                     YHVVTRVDLPTLVQGVEIYISSDGTSFSKLGDATRVSQSEVFEYGWNPGTLDAGDYTL
                     RARIVGTDQVDDRAITLDNALQTAEITDPRNLAQAVFIDREVTVAGTASAGAEGLTLY
                     YTKTGPNEARNETEWLECGVVVLPTGEQAQPFSGKCRLADTDYASQVTGVAAIATTCD
                     GTFGCEAPLGGASAETGDAHRVFGVPANPAVALSTAETGDAGSCQRVVVQLTDQAGDA
                     VPDSNIDVHLRGPNNKVGFCDLATGGSTRRSPDQGGHQVVSGETDEGNHSDTGVRHTE
                     GLTGSGGRFIVGIRSPSPGVSDLDAWFDVDDDDVRDAAEAAMDGSFEWTTPRDCTQLG
                     TPGDDVLTGTRGQDRMCGLGGDDILVGKGGKDVLLGGGGNDVMRGNGGADKLTGGKGN
                     DTLIGGGGKDKALGGPGADEVRGSGKNDNLAGGGGRDSLDGGPGSRDRCRGGPKKDRE
                     RRCER"
     gene            complement(7527..>8418)
                     /locus_tag="VNP73_09945"
     CDS             complement(7527..>8418)
                     /locus_tag="VNP73_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL66281.1"
                     /translation="GAPDKKHIEASGGGVGNAGTFAVRFYNEDTEGSTQITFWADEDD
                     DDRYCSSELAIAVSIGWDEPAPSPEIEAFELDVCPIPTPPPPGGSPSGTGSPSPSSSP
                     TGPPDPRGCTVTGTDGDDELRGTNGDDVICGLGGDDSIEGLDGDDVIHGDEGDDRILA
                     GTGNDSIDSGGGRDGVNGQGGNDTIDGGDDDDVLAGARGKDTLRGNNDSDSLIGGGGP
                     DILVGGSGNDIALGGPADDIVKGATGKDDLEGQNGADTIKGGPQNDRLDGGPGTDTLR
                     GGPGKDRCNGGSGRDRQNSC"
BASE COUNT         1402 a         2870 c         2562 g         1584 t
ORIGIN      
        1 agagtcccac cccggccggg aggtaggaaa acccccataa agggcgaacc ctccttttat
       61 gggaccttcg ggcacgcccg cgcgcgcgac cgatgatcgc gaactggttc ttgcctttca
      121 ggcaggcgag agggccgcgt acgacgagat gtatcgccgc tatcacggcc gcgtgtcagc
      181 gatctgctcg cggatactga acaactctca ggacgcggaa gaagcagcac aagagacttt
      241 cctgcgcgcg tatcaagcgc tccctcgctt caacggaaac ttcctgctgg gggcgtggct
      301 tgcccgcatc gcggccaacg cgagcgtcga tcaactccga accaagtccc ggagcccgct
      361 cgtcggcctg ccgagtgatc acgaggatcc gggtgacgac gtcgatcccg aggatgtcgt
      421 cgtcggcgag catccccggc tcgacatcgc actggccgag atcaagccgc tccatgcccg
      481 tgccctcgcg tggcggaacg tcgacggcct gtctcacctc gagatagccg agctcttgtc
      541 gatgactccg tcccaggtca aggctctgtt gcacagagcc cgtgtctcgt tgcggaaggc
      601 ctgggatcgt gccgaggggt gggcgatcgc tcccctgttc gggatcaggt ccttgtggcc
      661 cgagcgccac ggcaactccg gttcgttctg gaacgccgca ccatcgcttt cgcctttgct
      721 cgcggaaaag gtcgccgcga ccgcactggt cgtcgccgtc gcgttaagtg gcctgcccac
      781 gaacgcgagc gatacatcca ccccggccct cacctcgccg cgattgggca tcggtgccac
      841 atcacacggc tccatcgcct ccgccgaggt tcgccccagc ctcacgacct ccgaggtaag
      901 tgcgcccaac gagcccgcga gcggttcgga tgcggtcgcg gacctcaccg agaagatcga
      961 ggagataaag ggcacgctca acggccgcga gaagctcgac gaggacccac ctcgcccgga
     1021 agagcccggc gggaaggatc cgtcgaccga agccgcatcc acggcccgta aggtcgtaaa
     1081 gaaggtcaaa gacctgcttc ctcacctaag cgtggacacc agcctctaat cgcgcgacgg
     1141 ctcccctcga gcctggtata tccatcggat gcccgacacg ttggtacccc gaccgcccga
     1201 gcctccgccc tcgttcctgg agggggaccg gttcagcgtc ctgtgcgaga tagagccccc
     1261 acgccagccg gacatgagtc acgtccacga acaggtcgag gcattgaagc ctgcggtcga
     1321 cgctttcctt atccccgaca accatctggg tcgggccaca gtctcgagcg tttccgttgc
     1381 tcacgaggtg gcttacatgg gaggacgttc gatcgcatgc atgaacgcgc gcgatcggaa
     1441 cctactgggg ttcagacgcg accttctgac cgcagcttcc tacggggtcg atcacttcct
     1501 attcgtctac ggcgacgcgc cgcgggaagg tcgacgcact accgacctca cggtcagagg
     1561 gatgatcgat gaagtgcgcg gattcggatc gggcccactg ttccagggat accccgactt
     1621 ccgcatcggc accgtgacga agacctcacg acccctcgca tggcgacaac gcgccgacta
     1681 cgtgctcgtc cagatcacat tctcgttcga acggttgatg cgctggagag agtcgatcga
     1741 tttcgacggg cgcatctacc cggccgtgct cgtcctcgct agtagccgga tggcacgacg
     1801 gatcaacgca actctcagag acgtgcgcgt ccccgaccac atcatcgaca aactcgatga
     1861 cggcaagcac gtcggattgg atcttgcgtg cgaacagatc gaggcgatca aggattcagg
     1921 tgctttcgac ggtgtgcatc tcgtcccggt cggacgctac cgggagatgg ccgaacgact
     1981 gcagacctaa cggaaacggc cccggttcga accggggccg tttgtggatg atcggtcgtt
     2041 cttagcgaac ccgtttcgtc cgggacttcc ccgcctcaca cacgatgttg tcaccgttct
     2101 cgtcgatgaa ggtcttctgt gcggcacgcg cgtagtactt acccttagcg cgcggctcgc
     2161 ggatcctgta gttgccattc gcattcgtca cggccgttcc aaccttcggg tcgcgaccgc
     2221 gcttctgctt gcgaaggtcg atcgttcgct ccgacctgca accagatgct gccgaccgag
     2281 cggctcctct gaaagcactc cgcttgtacc tgatcgaaac ctgggtgggc tcgttcttgg
     2341 tctgtccgcc ccacccgatc tgaccgttcg ccgagatctc gtcctcgtca tcggcatcga
     2401 atacgtcgtc gccgtcgttg tcgagccaca cgagtaagtc cgtcgttccc tgtcgagccg
     2461 acgtgacacc gaacacgaaa gaaccgttcg cgtcggtcat cccctctatg tgaccggtgc
     2521 cgtcaccgtg gtatccgccc gcgtgctcgc cctggtccgg cgggaacgcg ttggagcctc
     2581 cctcgggagt acagaacgag accccctcgg gaccgagcgc atggacgtcg atgtcgcgcg
     2641 cgtccaatgc attgccacct tgacgcgcga cggccgtcat cttgacgcaa tcgccggttg
     2701 cggcagttgt agaactggga tcgaggatga gggtcttggg agcttgggga ggatccgtac
     2761 cccacccgag ctgagccccg ccagaagctt ccgtcgctcc ctgcagatcg tcgtcgttgt
     2821 cgtcggacca cgccgtgatc agcgtcccgc cctctaccga ggagcgcagc acgaaggtga
     2881 acgatccatc ggcacccgta ccggtggtgg actcgatgtg tttttggtcg gccgctcccg
     2941 gaacgttgtg gtcgccctga cttccctcgg cgtccgtctc ggcgcacttc acggtcggct
     3001 cggtgccgct gtgcgacttg tccggagcct ggaacgcatc ggacgtccct ctctgattcg
     3061 cgaacttgag ctgatcgtcc ggaccttggg cgtggatgtc gatgttgacg ccactgacca
     3121 cgttgccggc ctggtccaga gcggttgccg taactgcgaa gcatgtcccc tcgtcgacct
     3181 cctcggaatc cggatccatc cgaagcgagg taggcgtggc cccgtagacg accacccggt
     3241 gagcgtcacc ggtctcgtcc gccggtggca ccggcatccc cggtgatggt gtcgagttcg
     3301 cgacgagagc aacggccttg acgcgcgctg ccggaccgtc gtcctgaagg gtgcacttga
     3361 cgctgacatc gccctcgctg ggaactgcgc tcccacaggc gagccattcc ggcgcgccgc
     3421 cgacgttgct caccgaataa agagctcgga cctgactggc cccttcactc gtcgtcccct
     3481 cgatcaccgc acttgcagcc ttgccggggg ccgcatagaa gcccaattgg cccccgttct
     3541 caggatgggt gatctcgacc gtctcggctt cggaccccgg cgggatgtcg gcggcttgaa
     3601 caagcacgct catcggctcg gaacgatcga cttcatttcc cgtcccgacg ccggcgaagt
     3661 tctcgtacag gatcgcctgc aacgtgtact ggccgtcggc gactccctcc aggctcagtt
     3721 caccttccca agcatccgag ccggctctcg ttccgactac cgttgcttcg gtccccgctg
     3781 cattcgaaag ctcgaactcg acgagggcgt cggtgggcac ctctcccacc cagacgttca
     3841 ggtggtaggt gtcgtcggca tcctccttgt cggagatccg gatcgtcggc gagtaccccg
     3901 acgggttcag aaactccact gtgggagccg ccagtgcctc ggtcgcggga agaaccgcga
     3961 acagaagtac accggccgcc acggcgtggc caagagatcg catccgtttc atcgttcaca
     4021 tcgcctttca cggtccttct tcggacctcc acggcaccgg tcgcgggacc ccggaccccc
     4081 gtcgagcgag tcccggccgc ctccaccggc aaggttgtcg ttcttgccac ttccgcgcac
     4141 ttcgtcggca ccggggccgc ccaaagcctt gtctttaccg ccaccaccga tcagtgtgtc
     4201 gttgcccttg ccacccgtca gtttgtcggc tcctccgttg ccccgcatca catcgttacc
     4261 gccaccaccg agcagcacgt ccttgccgcc cttcccaacg aggatgtcgt cgccgcccag
     4321 tccgcacatc ctgtcctggc ccctggtgcc ggtgagcacg tcgtcgcctg gtgtaccgag
     4381 ctgcgtgcaa tcacgcggag tcgtccactc gaagctgccg tccatcgcgg cctcggcggc
     4441 atcacgcacg tcgtcgtcgt cgacatcgaa ccacgcatcg agatccgaca caccgggact
     4501 cggggaacgg atgccgacga tgaaccggcc cccggacccc gttaggccct ctgtgtggcg
     4561 aacgccggta tctgaatgat tgccctcgtc cgtctcaccc gagaccacct gatggccgcc
     4621 ttgatcgggc gacctgcgcg tgctgccgcc cgtagccaga tcgcagaacc cgaccttgtt
     4681 gttcggccct cggaggtgca cgtcgatgtt ggaatcggga accgcgtccc ctgcttggtc
     4741 ggtcaactgg accacgacac gctggcagct gccggcgtcc ccggtctcgg cggtcgagag
     4801 ggccacagcg gggttcgccg ggacaccgaa gacgcggtgg gcatcacccg tctcggccga
     4861 agccccgccc aagggcgcct cgcatccgaa tgtgccatcg cacgtcgtcg cgatggcggc
     4921 gacaccggtg acctggctgg cgtagtcggt gtcggccagt cggcatttgc cggagaaggg
     4981 ctgtgcctgc tccccggtgg gaagaaccac aacgccacac tcgagccact cggtctcgtt
     5041 gcgcgcctcg ttggggcccg tcttcgtgta atagagggtg agtccctccg cgccggcact
     5101 tgctgtaccc gcaacagtca cttccctgtc gatgaacacc gcttgcgcca ggttcctcgg
     5161 gtcagtgatc tcagcggtct gcagagcgtt gtccaatgtg atcgctcggt cgtcaacctg
     5221 atccgtgcct acgatccggg cacgcagggt gtagtcaccg gcgtccagcg tgccggggtt
     5281 ccacccgtac tcgaagacct cagactgaga gaccctcgtt gcatcgccca gcttcgagaa
     5341 cgaggtcccg tccgacgaga tgtagatctc gaccccttga accagggtcg gcaggtctac
     5401 acgagtcacc acgtgataca cggggtcggc atcctgcagg ttcgagaccg cgtccccggc
     5461 cccgccgtag cccgaaggat tcacgaagcg cactttcgcg tcctgggcac tcgcagccca
     5521 gctcatcgac gctgccgcgg tcggctcggt cgcagcaagc tcgtcgtcgt cggcaccagg
     5581 ctctccatcc acccatgccg tcaatgtcgt gtctccggaa acgggggaca tgagaccgat
     5641 aatcaggtcg ccttcttcgt tcgtcgttcc ctcggtgtgc tggatcgtcg gatcggtcgg
     5701 cgttgccccc gggtgctgtg cctcatccga ctcgcccgct tcggacgtgt gggttgcggg
     5761 ggacggagtg gcatcgggcg cctgcaatac agaaccgcct ccgggtgtgc agaagtcgag
     5821 atcgttcgat gggccgctcg cgtgaacgtc gacgttgaca ccgtcgagga ccgacttccc
     5881 ggcgcgcgtt ttcacggtga aaggcgtaca gctcccgatc aaaccggccg aaccgctggg
     5941 actgatcgcc aacgccaccg gcttctcgag ccattgcgcc ctggctatgg cgaaggcgtc
     6001 gaccttctcc ctcgtatcgc cgttcaagtc gcggagggcg tcgaccgtcg cagggttgtt
     6061 ctcctcgtcg acccatgccg tgatctgcat gtcgcccgcg ttaggagaaa agacatggaa
     6121 ccggaactgc cccggcgggt cctcgcccaa gccggtgccg gccatagctt cgaagtgctt
     6181 gacgtcgagc tcctccggct tctcgtgctt gccctcctgc ccgatgtgcc caccgggcga
     6241 gtcgcaatcc cacgcggggg caacgacgtg gctccccaga tcgggaggtt tgcgtgcact
     6301 ggagtcgccg cccacagccg agcccgcctc gtcgacggga ccaaccacgt gaacgtcgac
     6361 gttcgctcct tgaaccgggc gctggaactg atccaggacg gtcatgtcga aagcgaggca
     6421 accggtgttc gcctcgcgac gcgcgcccgc gggataggag gcatcgggtt cggttggttc
     6481 gaccgaactt atcgacgccg tcatcttcga agggtcctgt tggtagggca cgactgcatg
     6541 gacgtcgccg gagtcctggg tgaaaaagcc ctgtccggtc ggagtgtcgg gctcggccgc
     6601 gaccattgca acggacgtca ccgtggccgg atcggcagtg ggcggtaacg tgcatgtgac
     6661 ctgaaacgcc cctgtgggat ccacgaaccc gcaatcgccg gtgaattcag ggatctggcc
     6721 cttcggcgtg gtcgaaaaga acacgtaagc acgcgcgttc gcgctcgcta ctccgtcgag
     6781 aacgaaacgc cacggtcctg ctgtcgattt gaagaatcca aggccgccgc cgttgggcgg
     6841 ccatgtgatc tcgacggtct cttcggcaag atcgatctcg gccggagtcg ccgattcggc
     6901 gatcttggcc ccgccggaga agagtcgagc tatgactcta cccgtcgtcc cgtccagaga
     6961 ctcgggaacg tcccaatgcg cctcccaaac gccgggggaa tcttcgacct gttcgagggc
     7021 gccgatcgtc gtctcgccgc cgccgtcggc cgcataaacg gccgtagccg tcgctcccgc
     7081 cggcgcgccg ctggtcgaag cggtcacgtg ataagtctcg tcggcatcca ttaccttcga
     7141 gatctgtggg atgtgttccg atgcggcgct ggccgggggg gcggtgaaag ggtcgtatcc
     7201 ctcggccgga ttaagaagcg cgatcgtggt gcccgttgga gcaggtgaac ccgacggacc
     7261 tccgctgggc gaccccgagg ggctaccgct gggagagccg ctcgtttgcg agccggacgg
     7321 ttgggcaagg atgaccgggc ccgccccgcg cgagggactc gtaagaacga gaacgaggaa
     7381 caccgccgtg ccgatcacgg ccaaagcgcc gcgcgccagc agtcgatcgc cgctcaaaga
     7441 caagggttgc ctgaagaaac tcacccacca cacctccgcc tggtcctcag ttcgctaagt
     7501 atccttcgcc agccgtcaat gcggcactag caactgttct gcctgtccct gccggacccg
     7561 ccgttacaac gatccttgcc gggccctccc ctcaaagtgt cggtaccggg gccgccatcg
     7621 aggcggtcgt tctgggggcc gcccttgatc gtgtcggccc cgttctggcc ttcgagatcg
     7681 tccttaccgg ttgcgccctt aacgatgtca tcggcgggac ctccgagagc gatgtcgtta
     7741 ccggatcctc cgacaaggat gtcgggaccg ccgccgccta tcaggctgtc actatcgttg
     7801 ttgcctcgaa gagtgtcttt gcctctcgct ccggcaagca cgtcgtcatc gtcgccacca
     7861 tcgatcgtgt cgtttccacc ttgcccgttc acgccgtcgc gccccccacc cgaatcgatc
     7921 gagtcgttcc cggtccctgc gaggatccgg tcgtctcctt catcgccgtg gatgacgtcg
     7981 tcaccgtcca gtccctcgat ggaatcgtcg ccgcccagtc cacagatgac atcgtcgccg
     8041 ttggtgcccc tgagctcgtc atctccgtcg gtccccgtga ccgtgcaacc acgtggatcg
     8101 ggggggccgg tcgggctgga gctcgggctc ggggagccgg tcccggaggg cgagccaccc
     8161 ggaggtggag gcgtcgggat cggacagacg tctaactcga acgcttcgat ctcgggactc
     8221 ggcgccggtt cgtcccagcc gatgctcacc gctatcgcca gctcagacga acagtagcgg
     8281 tcgtcgtcgt cttcgtcggc ccagaacgtg atctgcgtcg aaccctcggt atcttcgttg
     8341 tagaagcgaa cggcgaacgt tccggcgttg cccacccctc caccggaagc ttcgatgtgt
     8401 ttcttatccg gcgcaccg
//
LOCUS       DATMED010000383         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4146345, whole genome shotgun sequence.
ACCESSION   DATMED010000383 DATMED010000000
VERSION     DATMED010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4146345"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1286)
                     /locus_tag="VNP73_09950"
     CDS             complement(75..1286)
                     /locus_tag="VNP73_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWL66282.1"
                     /translation="MSRDRLKPSVTGLPTAGAMLRLIRDGGAVTRSELTQLSGLSRST
                     VAQRIDALVASGLIREKAEGSSTGGRPPTILEFNHSAGVILAADLGATHSRVAATDLA
                     GIMLAETRHDIDVTRGPVEVLDWLEKTFLDLLKEAGHSRAAVRGIGIGVPGPVEYATG
                     RPVSPPIMPGWDGFPMPERFESFDVPVLVDNDVNIMALGEHWSRWRDCDHLIFVKVGT
                     GIGCGIVTSREIHRGAQGAAGDLGHIRATDDENVVCTCGNLGCLEAVAGGGALARRLT
                     EMGVPATNSRDVVGLVRAGQPQAVQLVRQAGRLLGEVLAAATNLLNPSVIVIGGDVAH
                     AHEQLFAGVRETVYQRSLPLATRHLRIVRSDLDDRAGIIGAAVMAIEHVFSPEAVDAA
                     IAVRSAEKSSA"
     gene            1408..>2534
                     /locus_tag="VNP73_09955"
     CDS             1408..>2534
                     /locus_tag="VNP73_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWL66283.1"
                     /translation="MPTPLRVAIAGTGFIGRVHARSALLAGATVVGVAASSPDRAEAA
                     AKDLGVERAFASADELVRADDVDVVHICTPNHLHFDLALAALDAGKHVICEKPLATQR
                     ADAERLVRAAKAAGKVASVPFVYRYYPTVREARARVEADDIGDVRLIHGAYLQDWLLD
                     PEDGNWRVDEALGGASRAFADIGSHWCDLIEFVTNDRIRRVTAKLLTAVPERFVGEHQ
                     DAFSGSKGAEKISVTTEDAAVVMFETRGGAIGSTVISQISAGRKNQLRFEIDGSKAAL
                     AFDQEAPETLWIGRREGPIVLPRAPELLSKGAATYATLPGGHPQGYHDCFDAFVNDTY
                     EAIHNGVPDGLPVFTDGLRAAAITEAVLTSAREGRPVDLEG"
BASE COUNT          453 a          832 c          816 g          433 t
ORIGIN      
        1 agctcggagc ccggggtact ccttggtgag acctctgacg ctgagaagaa cttccgtctc
       61 gttccgttcg tcggtcacgc gctcgacttc tccgcgcttc gaacggcgat agcggcatcg
      121 acggcctcag gcgagaagac atgctcgatg gccatcaccg cggcaccgat gatccccgcg
      181 cgatcgtcga gatcgctgcg gacgatgcgc aggtgtctcg tagccagcgg aagcgaccgc
      241 tggtagacgg tctctcgcac accggcgaac agttgctcgt gtgcgtgggc aacatcaccg
      301 ccgatcacga tcacggatgg attgagaagg ttcgtcgcgg ccgcgagaac ttctcccagc
      361 aggcgacctg cttgacgcac aagctggacc gcctgaggct gaccggctcg gacgagaccg
      421 acgacatcgc ggctgttcgt cgccggaacc cccatctcgg tcagcctgcg ggccaacgcg
      481 cctcccccgg cgacggcctc gaggcatccg agattcccgc acgtgcacac gacgttctcg
      541 tcgtcggttg cacggatatg gccgagatca cccgcggccc cctgggctcc acggtggatc
      601 tcacggctcg tcacgatgcc gcatccgatt cccgtcccga ccttgacgaa gatgaggtgg
      661 tcgcagtcgc gccacctaga ccagtgctcg cccagcgcca tgatattgac gtcgttgtcg
      721 acgagaacgg gaacgtcgaa ggactcgaaa cgctcgggca tggggaatcc gtcccagccg
      781 ggcatgatcg gtggactcac gggacgcccg gttgcgtatt cgaccggtcc cggcacccct
      841 atgccgatgc ctcgcacggc ggcccgactg tgaccggcct ctttgagaag atcgaggaag
      901 gtcttttcca accagtcgag gacctcgacc gggccccgag tgacatcgat gtcatgtcgc
      961 gtctccgcaa gcatgatgcc ggcgagatcg gtggccgcga cacgggagtg cgtggcaccg
     1021 agatcggcag cgaggatcac cccggccgaa tggttgaact caaggatcgt ggggggccgc
     1081 ccgccggtgg aggaaccctc cgctttctct ctgatcaggc cgctcgcgac gagggcatcg
     1141 atcctctggg cgaccgtcga ccgcgataac ccgctgagct gggtgagttc cgaacgagtg
     1201 accgccccgc cgtcgcggat caatcgcaac atcgcgcccg cagtcggaag tcccgtgacc
     1261 gatggtttta gacgatcccg cgacatagcg gtgaactaaa acacgtggac tttttatctg
     1321 tcaatgtcaa aagtccgcgc tcgagcgaag gtaaccatcg ggagttctgt ttgccgatag
     1381 caaaaggtcg ccggtaggat gctgccgatg ccaacccccc tccgagtagc gatagccggc
     1441 accggcttca taggtcgagt gcatgcccgt tccgcgctcc tggcgggggc cacggttgtg
     1501 ggggtcgcag cgtcctcgcc cgaccgggcg gaggccgctg ccaaagacct gggtgtcgag
     1561 cgggcgttcg cctcggccga cgagctcgta cgggccgacg acgtcgatgt ggtccatatc
     1621 tgcacaccga accacctcca cttcgacctg gctctggcgg ctctcgatgc cggcaagcat
     1681 gtgatctgcg agaagccgct cgcgacgcaa cgggccgatg ccgagcgctt ggtacgggcg
     1741 gcgaaagcgg ccggcaaggt cgcgagcgtg cccttcgtct accgctatta ccccaccgtc
     1801 cgagaagcga gggcccgcgt cgaagccgac gacatcggtg atgtgaggct gatccacggt
     1861 gcctatctgc aggactggct gctcgacccc gaggacggca actggcgggt cgatgaggcc
     1921 ctgggcggcg cttcccgagc cttcgcagat atcggctcgc actggtgcga cctgatcgag
     1981 ttcgtcacca acgaccgcat ccggcgggtg accgccaaac tgctgaccgc cgtaccggag
     2041 cgtttcgtcg gcgagcacca ggacgctttc tccggttcca agggggccga gaagatcagc
     2101 gtcacgacgg aggacgccgc ggttgtcatg ttcgagaccc gcgggggtgc gatcgggtcg
     2161 accgtgatca gtcagatctc ggccggtcga aagaaccaac tccgcttcga gatcgacggc
     2221 agcaaagcgg cgcttgcttt cgaccaggaa gctcccgaga ccctgtggat cggacgtcga
     2281 gagggtccca tcgtgctacc gcgcgctccc gaactgctgt ccaagggggc cgcaacctat
     2341 gcgaccttgc cgggcgggca tccccagggc tatcacgatt gtttcgatgc cttcgttaac
     2401 gacacctacg aagcgatcca caacggcgtt cccgatggac tccccgtctt caccgacggg
     2461 ttgcgcgccg cggcgatcac cgaggccgta ttgacctcgg cgcgcgaagg ccgccccgtg
     2521 gacctggagg ggtg
//
LOCUS       DATMED010000384         7821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4147406, whole genome shotgun sequence.
ACCESSION   DATMED010000384 DATMED010000000
VERSION     DATMED010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147406"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VNP73_09960"
     CDS             <1..272
                     /locus_tag="VNP73_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL66284.1"
                     /translation="GLSGGTYAHYFTLFLPATDVFYSLIKAATFATTVALIHCYYGFK
                     ASGGPAGVGVAVGKAIRASIVAVNILNLMLSSFMWGVEDTVRIAG"
     gene            339..1427
                     /locus_tag="VNP73_09965"
     CDS             339..1427
                     /locus_tag="VNP73_09965"
                     /inference="COORDINATES: protein motif:HMM:NF023315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWL66285.1"
                     /translation="MVAGSVVLTGLFLTGKVRPRVVPVSAEFVNCGQGLREGGDVKLR
                     GVLVGKIGVIELAGNGNDCRVNLDLFPQDVDQVPANAGAQIRAKTIFGEKWVEILTPD
                     PESSENIAAGDVIPRDRTIDPLEVETILNVALPLLEAIDPEYLAGTLEALSEGFVGHE
                     GAAIRGIEEGIKALEVPLNNEAEFKESIDQLAGTGETLSEVDDDLLVAMRNLDELNRF
                     TIENADLVSESLDRAPELLGELSTLFETRFGDLVELANSGATVLGIVSSRTGDLDRLL
                     NALPKFNSAWIRNLNHVCRYRQQTDEPGKATGDRVPGRCWRVHNLVTDSRGAYEPGKG
                     PGPERRSFETPDDVDSLLRAPAELGGAQ"
     gene            1424..2410
                     /locus_tag="VNP73_09970"
     CDS             1424..2410
                     /locus_tag="VNP73_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWL66286.1"
                     /translation="MRSVTTKLVTFTVFTIIVTFGLASLIGKISLFSDAYEIEAVFTD
                     ATGVLNGDPVKIAGVEVGKVTSFRVEGGEAILTMEIAGDVELPENVIADIKFRNLLGQ
                     RVLNLEEPKAPSTTALEEGGQIPVENTHPALDLAVVFNNLRPLIQSTNPEDINTVSRA
                     VLAIFKGHEKDLEGVLGNTGELTKELADGDQRLARLVENLDDVTRILNSESGSVRKGL
                     EQFTEFMEALAEVTPEIERTVDQLDTASGKFGGLVRKNRTNLDQELTDLATLLRVIEE
                     NLGPLDRVAKQLKEVLLATARTQSYGKWWNLYIVNLCVEGIPDTPTQLECSN"
     gene            2410..3384
                     /locus_tag="VNP73_09975"
     CDS             2410..3384
                     /locus_tag="VNP73_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWL66287.1"
                     /translation="MGVKNFRERSPVIVGIISIIAIALGTTGAFFIDRIPTVKRAYDI
                     EGEFADGAGLNVENQVRVAGIKVGTVSAVELDGDRVRVLMEIDNGTQIPRDAFAEIKL
                     ATLLGTKFVDIEAKGGGPFMESGDIIPLERTSVPYEIYQASNQGTNVLEGLDGPALNA
                     MLVELTKLTKVAKQEVGVALEGLNELGAGLNEKEDELRSLLAGADELTGVLDDKGPEL
                     VRLIDASNEVLGTVAQKREEVQSLLKVTRSMAAELANLVKDNRGKLDSILIDLHKALV
                     VLEKNIQHVDMALEYAGPSSRYFGSIWMQGRWGDIYGCAIVLSETCES"
     gene            3393..4847
                     /locus_tag="VNP73_09980"
     CDS             3393..4847
                     /locus_tag="VNP73_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWL66288.1"
                     /translation="MRPLSLAVVALLLAGGAGLLALRENEETYTITAYFTKAIGLFEN
                     SDVDILGVPVGKVTDIVPQGDRVKVVMELPTKHKVPDDAFAQIVPISVISDRYVQLAP
                     VYKGGPYLEDGAVLDVDRTQIPAELDDVFRQLKKLLEAIEPGEKGEPGALGDLIVQLD
                     KTLRDREDDLKGTLINAAALTDSLADAKQDLSGILVNLDGLFEQLSTRAGSLGELNSN
                     FAVVMTALAESNQDITGTLKNLGDLTHEVGDLVRDNGDRLGGDLERAAKITSTVLKNR
                     ASVEESLTWLPVVGEGLTKAHHPPPVSASDVRDNLVAAHCKALEDVPEPVRDVLKQIL
                     GEACGETPEQTADGPIAAPIADEPLLDCDEGVRKVKKQLDRVMKVDLPDRLVEDIVKP
                     MKKQIRRLRKQCDELGRFIDRPEDDELQELLDELLDDVPDVDPTDVPDLDELDPLGGA
                     ASGSAPAPTSRDKSAWKSFTDWMAGFVGFLGWAR"
     gene            4844..5926
                     /locus_tag="VNP73_09985"
     CDS             4844..5926
                     /locus_tag="VNP73_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWL66289.1"
                     /translation="MRRGLILTAILLPVALVASSCAALGFPNDCEGTEIIGIFEQIGD
                     LPEAANVQSSDVEIGDVDEIRLDGWTARLTMCLDPGMQIPSDVRAVVRTTSLLGEKFI
                     DLKPQSEGPPYLEDGDILDLNQTSKATELEDVFAQLAGILGTGNLEELNRFTAAQADI
                     LRDHTDEIRLVLSRLRKFTGLLAERKGEISTAVDSLDSVARTLKSDSSVLQNFLRSFA
                     DSSEVLANNKEGLQSLLFALDRFTNVSAQLLTQTERGINKQFEDLRPILRTVVGDSER
                     VAEILQTLATFTEYFPETMPGRYLQLDVCQAAPDMYNQGTTCPQSIANDDPKAAARSD
                     SDSPVAPENTAGYLLRLPLLGGRDGR"
     gene            5916..7115
                     /locus_tag="VNP73_09990"
     CDS             5916..7115
                     /locus_tag="VNP73_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWL66290.1"
                     /translation="MVGKRVKINMVAFLVLSLGFSYWLATQVLSSLQDRITVAVELDD
                     AGGVFTSQEVTYRGITVGQVGKMSVIEDGVRIELDINAANKIPKEDLEARVMFKSAVG
                     EQFVDLLPASSGPPFLQDGDVIPKGNTTIPVSTQELLTTVEAVLRGVPPEALKGAVDA
                     LGKGLTGRGPDLATIIESSADIAEVFADRSGELEGILRNGTQVGEAFLDSREDFATAI
                     RELATVSEILSSNTANLERLMREGNLTSREVVRLLREHNRGANDFLSDIAEINKIQAA
                     HGDDLDHLLRFLPSGLNGVNNTFESDTGLVRFSLVNDTNSPACSYGTARQSPSDRNTG
                     LPPKNARCEQSGQADTPASSDQPTSNQPSEDVPVPGIGSLPIDDAPVLPQRMSEWSWT
                     LFYLNAI"
     gene            7140..7721
                     /locus_tag="VNP73_09995"
     CDS             7140..7721
                     /locus_tag="VNP73_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66291.1"
                     /translation="MSEVAESIDTTNAEADKQPRKMLVPGLIAGLVVAVAVAATFGAL
                     WAQSQEIDPEEVASFMNAEAPEVDRVATEVINLLSNYDATTIDDVADRMLEVSTGNFR
                     TDYEETFAAGLGGALQEAEASTRGQILTGPDISFRSASEAVAVARVAETVQNKEIPTG
                     RSFEYLIEITLIDTVDGGWKADRVEILSGRETG"
BASE COUNT         1608 a         2391 c         2432 g         1390 t
ORIGIN      
        1 tggggctatc gggaggcacc tacgctcact acttcacgct cttcttaccg gcgacggacg
       61 tcttctattc acttatcaag gcggcaacct ttgcgacaac ggtcgcattg atccactgct
      121 actacgggtt caaagcatcg ggcggaccgg caggcgtggg tgttgcggtg ggcaaagcga
      181 tcagagcttc gatcgtcgcc gtgaacatcc tcaacctcat gctctcctcc ttcatgtggg
      241 gagtcgagga caccgttcga atcgcgggtt aacggttcgg ggaggaattc gagatcagga
      301 cgctgcggca ggcattgatc ggggtactag cccttgcaat ggtggcagga tcggtcgttc
      361 tgaccggcct gttcctcacc ggcaaggttc gcccgcgcgt cgtcccggtg tcggccgagt
      421 tcgtgaactg cgggcagggc ctccgagagg gcggcgacgt gaaactccgc ggcgtgctcg
      481 tcggcaagat cggcgtcatc gaactggccg gcaacggcaa tgactgccgg gtcaacctgg
      541 atctgttccc ccaggacgtc gatcaagtcc ctgcgaatgc cggggcccag atccgcgcga
      601 agacgatctt cggcgagaag tgggtcgaga tccttacccc agatcctgaa agttccgaga
      661 acatcgccgc cggcgacgtg atccctcgcg acagaacgat cgatcccctc gaggtcgaaa
      721 cgatcctgaa cgtggcgctg ccgctgctcg aggcgatcga ccccgagtat ctagcgggaa
      781 cgctcgaagc gttgtcggag ggctttgtag gacacgaggg agcagccatc cgcgggatcg
      841 aggaaggcat caaggctctc gaggtgcccc tgaacaatga ggccgagttc aaagagagca
      901 tcgatcaact cgccggaacg ggcgagacct tgagcgaggt tgacgacgat ctcttggtcg
      961 cgatgcggaa cctcgacgag ttgaaccgtt tcacgatcga gaacgccgat ctcgtgtctg
     1021 agagcctcga ccgtgcgccc gaactcctgg gtgaactgtc gaccttgttc gagactcgct
     1081 tcggtgacct ggtagagctc gccaacagcg gagcaacggt cctcgggatc gtctcgtcac
     1141 gcacggggga tctcgaccgg ctcctcaacg cgctccccaa gttcaactcg gcgtggatcc
     1201 gcaacctcaa tcacgtctgc cggtaccggc aacagaccga cgagcccgga aaggcaaccg
     1261 gggaccgcgt gccgggacgc tgttggagag tgcacaacct cgtcaccgat agccgcgggg
     1321 cgtacgagcc gggtaagggt cccggtccgg aacgccggtc gttcgaaacg ccggatgacg
     1381 tggacagcct gcttagggct ccggccgagc tgggtggtgc gcagtgagat ccgtcaccac
     1441 gaaactcgtc actttcaccg tcttcacgat catcgtcacg ttcggtctgg cgtcactgat
     1501 cggaaagatc agcctcttct ccgatgccta tgagatcgag gcggtgttca ccgacgcgac
     1561 gggggtcctc aacggagacc cggtgaagat cgccggcgta gaggtgggaa aggtgacgtc
     1621 gttccgcgtt gaaggtggcg aggcgatcct cacgatggag atcgccggcg acgttgaact
     1681 acccgagaac gtcatcgccg acatcaaatt ccgcaacctc cttggtcagc gggttctcaa
     1741 cctggaggaa cccaaagccc cgtccacgac ggcattggaa gagggaggcc agatcccggt
     1801 agagaacacg catccggctc tggacctggc cgtcgtattt aacaacctgc gtccgctcat
     1861 ccagtcgacg aacccggaag acatcaacac cgtgtctcga gccgtgctcg cgatcttcaa
     1921 gggtcacgag aaggacctcg agggcgtcct tgggaacacc ggcgagctga cgaaggagct
     1981 ggccgacgga gatcagcgcc tcgcgcgctt ggttgagaac ctggacgacg tcacgaggat
     2041 cctcaacagc gaatcgggct ccgtgcgcaa aggcctggag cagttcacgg agttcatgga
     2101 ggccctggcc gaagtcacac cagagatcga acgcaccgtc gatcaactcg ataccgcgtc
     2161 cggcaagttc gggggtctag tcaggaagaa ccgcacgaac ctcgatcaag agctgacgga
     2221 cctcgccaca ctcctgcgag tcatcgagga gaacctcggc cccctcgacc gcgttgcgaa
     2281 acagttgaag gaagtcctac tcgcaacggc tcgaacgcag agctacggca agtggtggaa
     2341 cctctacatc gtcaacctgt gtgtcgaagg gatccccgac acacccaccc aattggagtg
     2401 ttctaactga tgggcgtcaa gaacttccgt gaacgctcgc cggtgatcgt cgggatcatc
     2461 tcgatcatcg cgatcgcgct gggtacgacg ggcgcgttct tcatcgatcg catccccacg
     2521 gtcaagcgcg cttatgacat cgaaggcgag ttcgccgacg gagccgggtt gaacgtcgag
     2581 aaccaggtgc gggtcgcagg gatcaaggtc ggcacggttt cggcggtgga actcgacgga
     2641 gatcgcgtgc gcgtgttgat ggagatcgac aacggcaccc agatccccag agacgcgttc
     2701 gccgagatca agctggcgac gctcctcggc acgaagttcg tcgacatcga agcgaagggc
     2761 ggcggaccct tcatggagtc aggcgacata atcccgctcg agcggacgtc cgtcccctac
     2821 gagatctatc aagccagcaa ccagggaacc aacgtgctgg agggtctcga tgggcctgct
     2881 ctgaacgcga tgctcgtcga gctcaccaaa ctcaccaagg ttgccaagca agaggttggg
     2941 gtcgccctcg aggggttgaa cgaattgggg gccggactca acgagaaaga ggacgagctc
     3001 aggtcgttgc tagcaggggc ggacgagctg acgggggttc tggacgataa ggggccggag
     3061 ctcgttcgat tgatcgacgc atcgaacgaa gtgctgggta ccgtcgcgca gaaacgcgaa
     3121 gaagtgcagt cgctgctcaa agtgacgaga tcgatggccg ccgagctcgc gaacctcgtg
     3181 aaggacaacc ggggcaagct ggactcgatc ttgatcgacc tccacaaggc gctcgtcgtt
     3241 ctcgagaaga acatccagca cgtggacatg gcgctcgagt acgccggtcc atcgtctcgc
     3301 tacttcggaa gcatctggat gcagggacgc tggggagaca tctatggctg cgcgatcgtt
     3361 ctctcggaga cgtgcgaatc atgaagccct tcatgagacc gctctcgctc gctgtcgtcg
     3421 ccctcctgct cgcaggcggc gcgggtcttc tcgctttgcg tgaaaacgaa gagacctaca
     3481 cgatcacggc ttatttcacg aaagcgatcg gcctcttcga gaactcggat gtcgacatcc
     3541 tcggcgtccc cgtcggcaaa gtcaccgaca tcgttcccca aggggaccgg gtcaaagtcg
     3601 tcatggagct gccgacgaaa cacaaggttc ccgacgacgc cttcgcgcag atcgttccga
     3661 tctccgtgat ctccgacagg tacgtgcagt tggcccccgt ttacaagggc ggcccttatc
     3721 tggaagatgg cgccgttctc gacgtcgatc ggacgcagat acctgccgag ctggacgacg
     3781 tgttccgtca gctgaagaaa ctcttggaag cgatcgagcc gggcgagaaa ggtgagcccg
     3841 gagccctcgg tgacctgatc gttcagctcg acaagaccct gcgggaccgc gaggacgacc
     3901 tcaagggaac tttgatcaac gcggccgcgc taaccgattc gcttgcggac gcgaagcagg
     3961 acctctcggg gatcctcgta aacctcgacg ggttgttcga gcagctgtcg actcgcgcag
     4021 gttctctggg cgagctcaac agcaacttcg ccgtcgtgat gaccgctctg gccgaaagca
     4081 atcaggacat caccggcacg ctcaagaatc tcggcgatct gactcatgag gtcggcgacc
     4141 tcgtaagaga caacggcgat cgtttgggcg gcgacctcga acgcgcggcc aagatcacct
     4201 ccacggtgtt gaagaaccgg gcctctgtgg aggagtcact tacttggctg ccggtagtcg
     4261 gcgagggcct gacgaaagcg caccacccgc ccccggtctc tgcatccgac gtgcgcgaca
     4321 acctcgtggc tgcacactgc aaagctctcg aagacgtccc agagccggtg cgcgacgtgc
     4381 tgaagcagat cctcggcgag gcatgcggag agacgcccga gcaaaccgca gacggcccca
     4441 ttgccgctcc gatcgccgac gagccgttgc tcgattgcga cgaaggcgtg cgcaaggtca
     4501 agaagcagct cgaccgcgtg atgaaggtcg acctccccga caggttggtc gaggacatcg
     4561 tcaagccgat gaagaagcag atccgtcggt tgcgtaagca gtgtgacgag ctgggaaggt
     4621 ttatcgacag acctgaggat gatgagcttc aggagctgct cgacgagctc ctggacgacg
     4681 ttccggacgt agaccccacc gacgtgcccg atctggacga gctcgacccg ctcggcggag
     4741 ccgcttctgg ttcggccccc gctcccacct cgcgtgacaa gtctgcgtgg aagagcttca
     4801 ccgactggat ggcggggttc gtgggcttcc tggggtgggc gagatgaggc gcggactgat
     4861 ccttaccgcg atcctcttgc cggttgcgct ggttgcgtcg tcgtgtgccg cactcggctt
     4921 cccaaacgac tgcgagggga cagagatcat cgggatcttc gagcagatcg gtgatctccc
     4981 cgaagccgcg aacgttcaat cctcagacgt cgagatcggg gacgtggacg agatccggct
     5041 ggatggttgg accgctcgcc tgacgatgtg cttggacccg ggcatgcaga tcccgtccga
     5101 cgtacgggcc gtcgtaagaa cgacatcact gctgggcgag aagttcatcg acctcaagcc
     5161 tcagtcggag ggtccgccgt acctggaaga cggcgacatc ctggatctca atcagacgtc
     5221 caaggcgacg gagctggaag atgtcttcgc acagcttgcg gggatccttg gtaccgggaa
     5281 cctcgaggag ctcaatcgct tcaccgccgc acaggccgac atcctgcgcg atcacacgga
     5341 tgagatcaga ttggttctgt cgcggcttcg caagttcacg ggcctgctcg ccgaacggaa
     5401 gggcgagatc tcgaccgccg tcgattcttt ggattccgtc gcacggaccc tgaagtcaga
     5461 ctcgtccgtc ctgcagaact tcctccgttc cttcgccgac tcttccgagg ttctggcgaa
     5521 caacaaggaa gggctccagt cgttgctgtt cgcgctcgac cgcttcacca atgtcagcgc
     5581 ccaattgctc acccaaacgg aacgagggat caacaagcag ttcgaagatc tgcggcccat
     5641 ccttcgcact gtcgtcggcg actcggagcg agtagcagag atcctgcaga ccctcgctac
     5701 cttcaccgag tacttccccg agacgatgcc cggccgctat ctgcagctgg acgtttgtca
     5761 ggcggcaccc gatatgtaca accagggaac cacttgcccg cagtccatcg ccaacgacga
     5821 cccgaaggcg gcggcacgat cggattctga ttcgcccgta gcgccggaga acaccgccgg
     5881 atacctgctg cgccttccat tgctaggggg tcgcgatggt cggtaagcgc gtgaagatca
     5941 acatggttgc gttcctcgtg ttgtcactcg gattctctta ctggctggcg acgcaggtcc
     6001 tcagttcgct gcaagaccgg atcacggtcg cggtagagct cgacgatgcc ggcggtgttt
     6061 tcacctcgca agaggtcacg tatcgaggca tcacggtcgg acaggtcgga aagatgtcgg
     6121 tgatcgagga cggtgtccgc atcgagctcg acatcaatgc cgccaacaag atcccgaaag
     6181 aggacctcga ggcgcgcgtc atgttcaaga gcgccgtggg cgagcagttc gtcgaccttc
     6241 tgccggcttc ctcgggtccg cctttcctcc aggacgggga cgtcattccc aaagggaaca
     6301 cgacgatccc ggtcagcacg caagagttgc tcacgaccgt cgaagcggtg ctgagaggcg
     6361 ttccgccgga agcactgaag ggcgcggtgg acgcactcgg caagggcttg acgggacggg
     6421 ggccggatct cgcgacgatc atcgaatcgt cggccgacat cgccgaggtg ttcgccgacc
     6481 ggtccgggga gttggagggg atccttcgga acggcaccca ggtcggcgag gcgttcctcg
     6541 actcgcgcga agatttcgcc accgcgatca gggaactcgc caccgtcagc gagatcctct
     6601 ccagcaacac ggccaacctc gaacgtctta tgcgggaagg caacctcact tcgcgggagg
     6661 tcgtacggct tctccgcgaa cacaacaggg gggctaacga tttcctcagt gatatcgccg
     6721 agatcaacaa gatccaggcc gcgcacggag acgacctgga tcacctcctg aggttccttc
     6781 cctccggcct gaacggcgtc aacaacacgt tcgagtcgga cacgggtctc gttcgcttct
     6841 cgctcgtcaa cgacacgaac agccccgcgt gctcgtatgg cacggcgagg cagtcaccga
     6901 gtgaccggaa cacggggctc cctccgaaga atgcgcgctg cgaacagtcg ggacaggccg
     6961 acacgccggc aagttccgat cagccgacat cgaaccaacc gtctgaagac gtgccggttc
     7021 cggggatcgg ctcccttccc atcgacgacg caccggtgct cccccagcgc atgtcggagt
     7081 ggtcgtggac cctcttctat ctgaacgcca tctgatcccg gcacacctac actgcagcca
     7141 tgtcggaagt tgctgaatcg atcgacacaa caaacgcgga ggcggataag cagccgcgga
     7201 agatgctcgt tccggggttg atagccggac tcgttgtcgc cgtcgccgtg gccgccacgt
     7261 tcggagcgct gtgggcacaa tcccaggaga tcgatcccga agaggttgcg agcttcatga
     7321 acgcggaagc acctgaggta gaccgcgtgg ccaccgaggt catcaaccta ctcagtaact
     7381 acgacgccac cacgatcgac gacgtcgcag atcggatgct cgaagtgtcg accggaaact
     7441 tccgtaccga ctacgaagaa acgttcgccg ccgggctcgg gggcgctttg caagaagcgg
     7501 aggcctctac tcgggggcag atcctgacgg ggcccgacat ctcgttccgg tccgcctccg
     7561 aggcggtcgc ggtcgcccgc gtcgcggaga cggttcagaa caaagagatc cccaccggcc
     7621 gctcgttcga atacctgatc gagatcacac tgatcgacac ggtcgacggc gggtggaaag
     7681 ccgaccgtgt agagatcctc tccggtagag aaacgggtta agagccgaag gtttctacta
     7741 tcgcttggta gttctcatac atgcgttccg gcttctggcc gtaccacggg cccgcgcaca
     7801 tcaaccggtc ggcaccagct t
//
LOCUS       DATMED010000385         4405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_686768, whole genome shotgun sequence.
ACCESSION   DATMED010000385 DATMED010000000
VERSION     DATMED010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686768"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VNP73_10000"
     CDS             <1..348
                     /locus_tag="VNP73_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66292.1"
                     /translation="LAYLRTRHSFGTGDLARTTNHGTYLISLLRKLRQEVKTNPAALL
                     SWIQLTKRHTSFGMTSGEMFRLGVLASQIKPGKVRNITVPASTGSVGAASVVFISGNA
                     RSIYQRFRAKGSL"
     gene            complement(345..1916)
                     /locus_tag="VNP73_10005"
     CDS             complement(345..1916)
                     /locus_tag="VNP73_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HWL66293.1"
                     /translation="MANRSANQIVEDLAARFEGAEAEFHRAYWDSQIHASDENDRRRA
                     QAEIEVRALKGDPVAYRSVLDALEEEVHDPLIKRQLELLRASLAANQMDDRERAELVA
                     LGTSIESEFASFRPEVGGDLLSDNDIEEILKTSDEEATRRDVWLASKEIGGRVAGRIR
                     ELVRMRNKNARDLGYSDHYRMSLDLQELSEDWLFSVLDELEDLTQEPFERWKGELDAK
                     LAARFGTDHLAPWHYADPFFQHPPPDGRIDMDPFFDGGSAPDLAQRTFTGWGLDIGPV
                     LEASDLWPRIAKCQHAFCLNVDRSGDVRILANVVPGERWVEVLLHESGHAAYDLAIDP
                     HLPWVLRRPAHTFVTEAIAILTGALPKDPRWLTDVAGVSVEDVTRVTDRLRAARAGQK
                     ILFARWGLLMSHFERDLYSDPEIDLDARWWELVERFQSIATPSDVPKGSWASKIHIAV
                     APVYYHNYLLGEILAAQLRGAIEDQCGSLVANPAVGEFLRDRVFRHGQLLRWDGLVES
                     AVGKRLSARDLVASL"
     gene            complement(1944..2597)
                     /locus_tag="VNP73_10010"
     CDS             complement(1944..2597)
                     /locus_tag="VNP73_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWL66294.1"
                     /translation="MTVLVGGISLLLAVALAWFGADLSGAAARKARAQIAADAAALAA
                     VAESTPFGGGRPKAIASRYARLNGATLVGCLCPVGATAVQVTVEVEEVTAQARAVFDP
                     NAVMAAGGEGALDPALASAVGQLLDASAGRVTLVSGYRTHAEQENLWAEALARHGDAE
                     TADDWVAPPGSSMHERGLAVDLGGDLELAARLIERLDLPLVRPLANEPWHFELAVRV"
     gene            complement(2594..2968)
                     /locus_tag="VNP73_10015"
     CDS             complement(2594..2968)
                     /locus_tag="VNP73_10015"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HWL66295.1"
                     /translation="MKNPQGGQATVELAFCLPFVALLIAVVLEICLLGTDQIRLWNAA
                     REAARLATVQSDEAAIREAATAGGPERVAITMHPPAGNRTQGGTVTVRLIHRPKGHVP
                     LVRALVSRLTLRADATMRIEQP"
     gene            complement(2978..3154)
                     /locus_tag="VNP73_10020"
     CDS             complement(2978..3154)
                     /locus_tag="VNP73_10020"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HWL66296.1"
                     /translation="MDRLLALLRSQSGQTTAEYALVLLVAALVVGVFATFVKSGSLNE
                     VFEHVVRGLVDRAI"
     gene            complement(3242..3754)
                     /locus_tag="VNP73_10025"
     CDS             complement(3242..3754)
                     /locus_tag="VNP73_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWL66297.1"
                     /translation="MIHRLAILLTVVGLLSSCGGAGREAEEAKGTLCDPSEPLPSPSP
                     SFGPARALIDTEEGSVLVDLVIAETDEQQQYGLMHRDSFPEDCGMAFLYFEEHSGGFW
                     MKNTRIPLSIAFFDDEGEILAILDMEPCEKDPCEVYDPGVAYIGALEVNQGRFEDWGV
                     RVGDQFSITR"
     gene            complement(3785..>4405)
                     /locus_tag="VNP73_10030"
     CDS             complement(3785..>4405)
                     /locus_tag="VNP73_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWL66298.1"
                     /translation="AAAVAAASGARVSGRLVAGLPIAFLVLMPFRRVEMLDRADLYLV
                     LAGVGLAVAGMRWIDKLVPDPQGFDDPLLSFVSNIERSIGAGLGIRTALSTSTERSTD
                     ERLRVVAALVSAGATWHVALQRSPDPFLRALAPIVSRAERLGTPVAFEIDRLLVARRD
                     ELARLFDIQLRRAPVLMMVPLTLCVLPAYALLAFGPFLRGLGIPMG"
BASE COUNT          910 a         1425 c         1340 g          730 t
ORIGIN      
        1 cttgcctatc tccgaacgcg acactcgttc ggaacgggtg acctcgcgcg cacgacgaat
       61 cacggcactt acctgatctc tctgctgagg aaactccgtc aagaagtgaa gacgaacccg
      121 gcggcgcttc tcagctggat ccagttgacc aaacgacaca ctagtttcgg gatgacatcg
      181 ggtgagatgt tccggctggg tgtgctcgcc tctcagatca agcccggcaa ggttcggaac
      241 atcacggtgc ccgccagcac gggatcggta ggagctgcaa gcgtcgtgtt catatccggc
      301 aacgcacgat cgatctatca gcgcttccgg gccaaaggaa gtctctagag ggacgcgacg
      361 aggtcgcggg ccgagagcct cttgcccacg gccgactcca ccaatccatc ccaacgtaag
      421 agctggccgt gacggaacac gcggtcccta agaaactctc cgactgccgg attcgcaacc
      481 aacgaaccgc actggtcttc gatcgcgccg cgcagttgcg cggccagtat ctcgcccaaa
      541 aggtagttgt ggtagtagac gggcgcgacg gcgatatgga tcttcgacgc ccatgagcct
      601 ttcggaacgt ccgacggtgt tgcgatgctc tggaatctct cgacgagctc ccaccatcgg
      661 gcgtcaagat cgatctcggg gtccgagtac agatcgcgct cgaaatgaga catcagaagt
      721 ccccaacgag cgaacaggat cttctgcccg gcgcgagcag cacgcaggcg gtcggtcacc
      781 cttgtgacgt cctcaactga gacaccggca acgtccgtca accatctcgg atccttcggc
      841 agagcgccgg tgaggatagc gatcgcttcg gtgacgaacg tatgggcagg tcgtctgaga
      901 acccacggaa ggtgtggatc gatggccaag tcgtacgccg cgtgacccga ctcgtgtaac
      961 aggacctcga cccagcgctc tccaggaacg acgttcgcga gtatccgaac gtcgcccgat
     1021 cggtcgacgt tcagacagaa ggcatgctgg cacttcgcga tccgcggcca caggtcgctc
     1081 gcttcgagga cggggccgat gtccagaccc catccggtga acgtgcgctg cgcaagatcg
     1141 ggtgcggacc ctccgtcgaa gaacggatcc atgtcgatcc tgccgtccgg aggcgggtgc
     1201 tggaagaacg gatccgcgta atgccacggg gccagatggt cggttccgaa cctcgcagcc
     1261 aacttggcgt ccagttcgcc cttccatcgt tcgaacggtt cttgagtcaa gtcctcgagc
     1321 tcatccagga cggagaagag ccagtcctcg gaaagctcct gaagatcgag agacatccgg
     1381 tagtgatccg agtaaccgag atcgcgcgcg ttcttgttcc gcatgcggac gagctctcgt
     1441 atccggccgg caacccggcc cccgatctct ttcgaggcga gccacacgtc ccgccgggtt
     1501 gcctcctcgt cagacgtctt caggatctct tcgatgtcgt tgtcgctcag gaggtcgccg
     1561 cccacctcgg ggcggaagga ggcgaactca ctctctatcg aggtgcccag agcaacgagc
     1621 tcggctcgct cgcggtcgtc catctggttg gcggccagag aagcccgcaa cagttcgagc
     1681 tggcgtttga tcagcgggtc gtgaacctct tcctcgaggg catccagcac gctccggtag
     1741 gcgaccgggt cccctttcag agcccggacc tcgatctcgg cctgcgcccg gcgacgatcg
     1801 ttctcatccg atgcgtggat ctgcgagtcc cagtaggcgc gatggaattc ggcctcggcc
     1861 ccctcgaaac gggccgcgag gtcctccact atctggttcg cagatctatt cgccacgacc
     1921 tacacctacc cagcgataaa cgactacacc cgaactgcca gctcgaaatg ccagggctcg
     1981 ttcgccaacg gccgaacgag aggaagatcc aaccgctcga tcaacctcgc agcaagctca
     2041 aggtccccac ccagatccac cgccagccca cgctcgtgca tggaactgcc cggtggagct
     2101 acccaatcgt cggccgtctc ggcgtcgccg tgacgcgcga gcgcctcagc ccacagattc
     2161 tcttgctcgg catgcgtgcg ataacccgac acgagggtca cccggcctgc tgaagcgtcg
     2221 agcagctgtc cgaccgcgga ggcaagggcg ggatcgagtg cgccctcccc tccggccgcc
     2281 atcactgcgt tgggatcgaa gactgcgcgc gcttgagccg tgacctcctc cacctcgacg
     2341 gtcacctgca cagcggtcgc gcccacagga cacagacatc caacgagagt ggcgccgttc
     2401 agacgcgcat acctggatgc gattgccttc ggtcggcctc cgccgaatgg agtggactcg
     2461 gcgaccgcag cgagggcggc agcgtcggcc gcgatctgag cgcgagcctt ccgcgctgcc
     2521 gcgcccgaca ggtcggcccc gaaccaggcc aatgcaacgg ccaggagaag cgagatccca
     2581 ccaacgagaa ccgtcacggt tgttcgatcc gcatagtggc gtcagcacga agagtgaggc
     2641 gcgacacgag agcgcgcacg agaggaacgt gtcccttcgg acggtggatc aggcgaaccg
     2701 tgacggttcc gccctgggtt cggttccccg ctgggggatg catggtgatg gcaacccgct
     2761 cgggcccgcc ggctgtggcc gcctcgcgga tcgcggcctc gtcagactgg acggtggcca
     2821 agcgggccgc ttcgcgagct gcgttccaca gacggatctg atccgtgccg agaagacaga
     2881 tctccaacac gactgcgatc aacagcgcga cgaagggaag gcagaaagcg agctcgaccg
     2941 tggcttgccc gccctgaggg ttcttcaccg tctgtatcta gatcgctcga tcgacgagtc
     3001 ctcgaaccac atgctcgaat acctcgttca gcgagccgga cttgacgaaa gttgcgaaca
     3061 ccccgacgac gagagcggca acgaggagaa cgagtgcgta ctcggcagtc gtctgacccg
     3121 attgcgaacg aagaagagcg agaagtctgt ccacggttcc tcccgaggga aggccggtcg
     3181 gtcgccggcg tgtaaccgaa tgtgaacgtg attcgggtta aagcgctgtg actgagatca
     3241 cttagcgagt gatagagaac tggtctccca cgcgaacgcc ccaatcctcg aagcgacctt
     3301 ggttgacttc caaagcccct atgtaagcga ctcccgggtc gtagacctcg catgggtcct
     3361 tctcacaggg ctccatgtcg aggatcgcga ggatctcgcc ctcgtcatcg aagaaagcga
     3421 tcgacagagg gatgcgggtg ttcttcatcc agaacccgcc gctgtgttct tcgaagtaca
     3481 ggaaagccat gccacagtcc tcggggaacg aatctcgatg catcaggccg tactgctgtt
     3541 gttcatcggt ctctgcgatc acgagatcga cgagcaccga cccttcctcg gtatcgatca
     3601 gagcacgcgc cggaccgaag ctcggcgacg gagaagggag cggctcgctc ggatcgcaga
     3661 gtgtgccctt cgcctcttcg gcttcgcgcc ccgccccccc acacgaagac agaagaccaa
     3721 caacggtcaa gagaatcgcc aaccgatgaa tcatgagaac aggttgccat gacggaggga
     3781 cagatcaccc catcgggata ccgagcccac gtaggaacgg tccaaaggcg agcagggcgt
     3841 atgcgggaag aacgcaaagc gtcaggggca ccatcatgag aaccggcgcc cgccgaagtt
     3901 gtatgtcgaa caggcgcgcg agctcgtcgc gccgggcgac aagcagccgg tcgatctcga
     3961 aggcgaccgg agtgccgagc ctctcggccc gggacacgat cggagcgaga gccctgagga
     4021 acggatccgg gcttcgttgc agagcaacat gccacgttgc tcccgccgac accagagccg
     4081 caaccacgcg aaggcgctca tcggtcgatc tctctgtcga cgtacttaga gctgtgcgta
     4141 tccccaaccc ggctccgatc gaacgctcga tgttggatac gaaactcaga agagggtcgt
     4201 cgaacccttg ggggtccggg acgagcttgt caatccatct catgcccgca accgccaggc
     4261 cgacccccgc gaggacgaga tagagatccg cgcggtccaa catctcgacc cgccggaacg
     4321 gcatcaatac gaggaatgcg atcggaaggc cggcgaccaa tcggccggaa acgcgcgctc
     4381 ccgacgcagc ggcgaccgct gccgc
//
LOCUS       DATMED010000386         6858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5679072, whole genome shotgun sequence.
ACCESSION   DATMED010000386 DATMED010000000
VERSION     DATMED010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5679072"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VNP73_10035"
     CDS             <1..397
                     /locus_tag="VNP73_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HWL66299.1"
                     /translation="EKLAKMSAEELEAIEGVGPIIAQAIAAYFAEPRNREVLARLREG
                     GVQPVAPAAKKEGPLTGKTFVLTGGLEGFSRDEATRAIEDRGGKVTSSVSKKTNYVVV
                     GENPGTKYDKAVQLGVETLDEAALKKLLG"
     gene            813..1739
                     /locus_tag="VNP73_10040"
     CDS             813..1739
                     /locus_tag="VNP73_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWL66300.1"
                     /translation="MPSLDGKVAIVTGAGRGIGREHVLALAKAGAKIVVNDLGGSTHG
                     EGSDQTPAQQVADEVKALGGEATANFDNVADFQGAANMVKQAVSDFGRLDILVNNAGI
                     LRDRMLVSMSEEEWDAVIAVHLKGHFAPTQHAASYWRAQSKEGNQINGRVINTSSPSG
                     VFGNVGQTNYGAAKAAIAAFTIISAQELTRYGVTVNCLAPNARTRMTEDIFAGMPEPK
                     EGEFDPLDPSNISPIVVALAADDAQNITGQCFFVYGPTINVLKPWDVGVSVTKDGPWD
                     PDELLGELKKKFPEGIAPEGMMKMLGKASGQG"
     gene            1739..2185
                     /locus_tag="VNP73_10045"
     CDS             1739..2185
                     /locus_tag="VNP73_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWL66301.1"
                     /translation="MAEEFIDFELPVGADAVTEFASAVGSSDPMHLDDEAARRHGFPG
                     TLAPPTFTTRQIFQVPRDEREQRLGANLDYARVLHGEQEFVYKRLPIAGETLKGRMRI
                     SKDFEKEGKRGGSMRFVTYESVFTDSQGEEVLTAYYTLLQTAKDVG"
     gene            2292..2732
                     /locus_tag="VNP73_10050"
     CDS             2292..2732
                     /locus_tag="VNP73_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWL66302.1"
                     /translation="MTVIEEGTQLDEKVFGPLTREDFVRYANVSGDDNPIHQDEEYAR
                     KSGAPTVFAMGMLNAGYLATAVSEWFGGAQNLRRFKVRFTNRVWAGDEVVCSGRVVSV
                     QKGIATVALEARRRGKGPAELSLDEEQTAITGEAEIELSEGGNV"
     gene            2732..3553
                     /locus_tag="VNP73_10055"
     CDS             2732..3553
                     /locus_tag="VNP73_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWL66303.1"
                     /translation="MPLNKELIGKTYDAATFEVTADAIEKYARATNDLNERYLAGDDS
                     VASPIFPIVPSFQFMMQAGMDPDLQADLMRLVHGEEEHVLHRPIRPGDTLTAAPVIES
                     IETKDTGETFTVRIDLRNQNDEDVATVRATSFIRGSGPGSKSASAGDEPQREIVHEDS
                     VKVDDDQTIRYAAASGDPNPIHQDENIAKMAGLPGIILHGMCTMAIATKGAVDGLAGG
                     DPTKVKRVRVRFSKPVFPGQELTTRFWKQADNVYGFETYNPDGQVVIKNAEVELV"
     gene            complement(3550..4302)
                     /locus_tag="VNP73_10060"
     CDS             complement(3550..4302)
                     /locus_tag="VNP73_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66304.1"
                     /translation="MTQAMPETAVPEPLDGWRRDLLDAARKLSATMMAGFVTGLLVGG
                     IGSRLAMLILRLTSGPGVQGLESDDGFIIGRISPDSFFLIGLGVVSGLMTALIYVTIR
                     AWFPERLRGPMMAVLLGIVGGSLIVHPDGVDFVVLEPRWLAIALFVVVPAAQGWLTGI
                     LVERFLREESRFKKVPAWLALIPILVLAIGGLAGPAILLGAVALWAVNRAQPVFGAVW
                     LSEPVAWLGRTAVAVVTAFATVALVRDAIEIL"
     gene            complement(4312..5184)
                     /locus_tag="VNP73_10065"
     CDS             complement(4312..5184)
                     /locus_tag="VNP73_10065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWL66305.1"
                     /translation="MSTTPEPPAPTSVEPEEMTYCYAHKDTPTRLRCTRCDRLICGRC
                     AIPASVGQHCPECVAEAQKSAPKVRSAIRATAPVVFTILVINVVVWLTQSFVYRDLTF
                     RFGAIPIQIAAGEWYRLLTPMFLHAPVAIWHIGFNSYALFLFGPNVEQAFGHVKFLIL
                     YLIAGFSGSAMSYAFGSCASVGVGASGAIFGVLGALLVFLYNRRRSTFVRAYMKNVVF
                     LIVINLVIGQIMPNIDNLAHLGGLIGGLGVGAAFDNPPGRKFPAGVQALLAAAVLLAA
                     VGLVMWRTSNFVCG"
     gene            5225..5548
                     /locus_tag="VNP73_10070"
     CDS             5225..5548
                     /locus_tag="VNP73_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66306.1"
                     /translation="MPVRVATMNLHHAGGIDGRVDLERCAEVLKSTRSELFALQELDR
                     NLERSGRVDQPGLLAGLLGMPVHFHPTLTRGEGEYGIAIAAEGLSEISEEDLPRLGDE
                     EPAPL"
     gene            5548..5886
                     /locus_tag="VNP73_10075"
     CDS             5548..5886
                     /locus_tag="VNP73_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWL66307.1"
                     /translation="MARWRGFTVIATHLSPVRAARRTQLARLVELAREPADPVLILGD
                     LNCGRLGLGSLAAAGFRGRGLGPTTERGWPRRIDHVLAGPGARVVVAKVIGSGVSDHR
                     AVVAEVARTR"
     gene            5956..6435
                     /locus_tag="VNP73_10080"
     CDS             5956..6435
                     /locus_tag="VNP73_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66308.1"
                     /translation="MAHVTMQDVSTMVRPARARFPWRGLLMGALAVTAAAGIAWAAVP
                     RPASSDPDLTAVRTQAAAATEAVARLEAQVADLKADLRALDRSGDRTGARLGKATTAL
                     WGSLDRLRTSLKDQRGATSAANQEALAALERAESAARNLSVLEDRFEYHLRSDHGGG"
     gene            6436..>6858
                     /locus_tag="VNP73_10085"
     CDS             6436..>6858
                     /locus_tag="VNP73_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWL66309.1"
                     /translation="MRLLRDLVGQTLSGRYRLVARLAGGGMGEVFRGHDLLLDRPVAI
                     KILQPSLASDPDLVERFKQEARAAARLNHPNIVSVYDWGAEDDETYYMVMEYVSGTDL
                     REVITLRAPLEPGQAVDTMSVVCDALAAAHASGLVHRDV"
BASE COUNT         1372 a         2100 c         2278 g         1108 t
ORIGIN      
        1 cgagaagctg gccaagatgt cggccgagga actcgaggcg atcgaggggg tcgggccgat
       61 catcgcccag gcgatcgccg cgtacttcgc cgagccgcgt aaccgggagg tccttgctcg
      121 gctacgagag ggcggtgtgc agcccgttgc accggccgcc aagaaagagg gcccgctgac
      181 gggaaagacg ttcgttctga ccggcggatt ggagggtttc agtcgcgacg aggcaacgcg
      241 cgcgatcgag gatcgcggag gcaaggtgac atcgagcgtg tcgaagaaga cgaactacgt
      301 ggtcgtgggt gagaaccctg gtacgaaata cgacaaggcc gtccagctag gggtagagac
      361 cctcgacgaa gccgccctga agaaactcct ggggtgaaaa gaaaccggcg cgggctagac
      421 ccgcgccggt tttgtatcca gttctatcgc ctgccgcact tcgcctgggg cgcccagtgg
      481 accgagctgg gacggcgttg acgacggtgt accggttggg attctgccga aaggccctga
      541 agctgaggtg gccagaatcc aacttgaggc ttcagacctt tagcagccgg tcacctcacg
      601 aggtccgtcg gaactatgac tcaagtctgg accacctcct ttctcgtgtg taccgataat
      661 aaaaccacag gcagaatcct ttattccgca aatccgcggc ccccgtcccc tgacccatgt
      721 acagcgttct gcggatagag ttcgttgaca tttcgcggcc tccgccgcag tgcagcggcg
      781 agcgggcacc aacgatcggg aggttggaaa aagtgccgtc attggatgga aaggtagcca
      841 tcgtcacggg cgccgggcgg gggatcgggc gggagcacgt cctggcgctg gccaaggcgg
      901 gggccaagat cgtggtcaac gacctcggcg gttcgaccca cggggagggc tccgaccaga
      961 ctccggccca gcaagtcgcc gacgaggtta aggctctggg cggcgaggcc accgccaact
     1021 tcgacaacgt ggctgatttc cagggtgcag cgaacatggt caagcaggcc gtgtccgatt
     1081 tcggccggct cgacatcctc gtcaacaacg cagggatcct gagggaccgg atgctggtgt
     1141 caatgtccga ggaggagtgg gacgccgtga tcgccgtcca cctcaagggc catttcgccc
     1201 ccactcagca cgccgcttcc tactggcgtg cacagtccaa agagggcaat cagatcaacg
     1261 gtcgggtgat caacacctcg agcccgtcgg gtgtcttcgg caacgtgggt cagacgaact
     1321 acggcgcggc gaaggccgcg atcgcggcat tcaccatcat ctcggcgcag gagctcaccc
     1381 gttatggcgt gaccgtcaac tgtctcgctc cgaacgcccg cacgcggatg accgaggaca
     1441 tcttcgccgg catgccggag cctaaggagg gcgagttcga cccactcgat ccgtcgaaca
     1501 tctctccgat cgtcgtcgcg ttggccgccg acgatgctca gaacatcacc ggacagtgct
     1561 tcttcgtcta cggaccgacg atcaacgtgt tgaagccgtg ggacgtcggc gtgtcggtaa
     1621 ccaaggacgg accctgggat ccggacgagt tgctcggcga actgaagaag aagttccccg
     1681 aggggatcgc gcccgagggc atgatgaaga tgcttgggaa ggcctcgggg caaggctgat
     1741 ggcagaggag ttcatcgact tcgagttgcc ggtcggcgct gacgccgtca cggagttcgc
     1801 gtcggccgtc ggcagctccg acccgatgca cctcgacgac gaggctgcgc gccggcacgg
     1861 cttcccaggg acgctcgcgc caccgacctt cactacacgg cagatcttcc aggtgcctcg
     1921 cgacgagcgg gagcaacgac tgggtgcgaa cctcgactat gcccgcgtcc ttcacggtga
     1981 gcaggagttc gtctacaagc gcctgccgat cgcgggagag acgctcaagg gccggatgag
     2041 gatcagcaag gacttcgaga aagaggggaa acggggcgga tcgatgcgct tcgtgaccta
     2101 cgagtcggtc ttcaccgatt cccaaggcga agaagtgctg acggcctact acacgctgct
     2161 ccagacggca aaggatgtcg ggtgaggagg gggttcgggg gaaccagtga ggccgcttgc
     2221 ggccggggtt cccccgattc cggagatcgg ggacacctct caggtgaacc cgatcgcagg
     2281 aggaatgaaa aatgacggtc atcgaagagg gaacgcagct agacgagaag gttttcggcc
     2341 ccctcacgcg cgaagacttt gttcgctacg cgaacgtatc cggtgacgac aacccgatcc
     2401 accaggacga ggagtacgca cgcaagagcg gcgcacccac cgtctttgcg atggggatgc
     2461 tcaacgccgg ttacctggca acggcggtga gcgagtggtt cggcggtgcc cagaacttgc
     2521 gtcgtttcaa agtgcgcttc acgaatcggg tgtgggcggg tgacgaggtc gtctgctcgg
     2581 gacgtgtcgt gtcggtccag aaggggatcg cgacggtggc gctcgaggcg agacgacgcg
     2641 gcaagggtcc cgctgagcta agccttgatg aagagcaaac ggccatcacc ggcgaagcag
     2701 agatcgagtt atccgaaggg ggaaatgtct gatgccgctg aacaaggaac tgatcggcaa
     2761 gacatacgac gctgcgacgt tcgaggtgac ggccgacgcg atcgagaagt acgcccgcgc
     2821 cacgaacgat ctgaacgagc ggtatctcgc cggggacgac tcagtggcgt cgccgatctt
     2881 cccgatcgtt ccttccttcc aattcatgat gcaggctggt atggacccgg acttgcaagc
     2941 cgacctcatg cgcctcgtgc atggtgaaga ggagcatgtt ctgcaccgac cgatccgacc
     3001 cggcgacacg ctcacggcag ctccggtcat cgaatcgatc gagacgaagg acaccgggga
     3061 gacgttcacg gttcggatcg atctcaggaa ccagaacgac gaggacgttg caaccgttcg
     3121 agcgacgtcg ttcatccgtg gctcgggccc aggatcgaag tcggcatctg caggcgacga
     3181 gccccaacga gagatcgttc acgaggattc ggtcaaagtc gacgacgatc aaacgatccg
     3241 gtacgcggcc gcctcggggg acccgaaccc gatccatcaa gacgagaaca tcgccaagat
     3301 ggccggcctc ccggggatca tcctccacgg catgtgcacg atggcgatcg cgacgaaggg
     3361 agccgtcgac ggactggccg gtggcgaccc gacgaaggtc aaacgggtca gggtgcgttt
     3421 ctcgaagccc gttttccccg gtcaggagct gactacgcgc ttctggaagc aggccgacaa
     3481 cgtttacggc ttcgagacct acaacccgga cggacaagtc gtgatcaaga atgccgaagt
     3541 ggagctcgtc tagaggatct cgatcgcgtc gcgaacaagt gcgacggtcg caaacgcagt
     3601 aacaacggcc acggccgtgc ggccgagcca ggcgacgggc tccgacagcc acaccgcgcc
     3661 gaaaaccggt tgagcgcggt taaccgccca cagcgcgacc gcgcccagca ggatggcggg
     3721 acccgccagg cccccgatgg cgaggacgag tatcggtatg agggccagcc acgccgggac
     3781 cttcttgaac cggctttcct cgcgcaggaa tctctcgacc aggatgcctg tgagccagcc
     3841 ctgagccgcc ggaaccacca cgaacagcgc gatcgccagc caccgcggtt cgagaaccac
     3901 gaagtcgacg ccgtcgggat ggacgatcag cgaaccgccg acgatcccga gcagcacagc
     3961 catcattggg ccgcggagcc gctcggggaa ccacgcccgg atggtcacgt agatcagcgc
     4021 ggtcatgagc ccgctgacca cgcccagccc gatcaggaag aaggagtccg gggagatccg
     4081 gccgatgatg aagccatcgt ccgactcgag accttgcacc cctggaccgg acgtcaggcg
     4141 aaggatcaac atcgccaggc ggctgccgat gccgccgacg agcaaccccg ttacgaagcc
     4201 ggccatcatc gtcgccgaca gtttgcgagc ggcgtccagg agatctcgtc gccaaccgtc
     4261 cagaggttca gggacggcag tttctggcat ggcctgcgtc atgcctctca actaaccaca
     4321 aacgaagttt gaagtcctcc acatcacgag gcccaccgca gcgagtagca ccgctgctgc
     4381 caatagggcc tgaacgccgg cggggaactt gcgccccggc gggttatcga aggcggcacc
     4441 gacgccgagc cctccgatca agccaccgag gtgagccagg ttgtcgatgt tcggcatgat
     4501 ctgcccgata acgagattga taacgatcag gaaaacgacg tttttcatgt aagccctgac
     4561 gaacgtcgac cgcctgcggt tgtagaggaa cacgaggaga gcccccagga cgccgaagat
     4621 cgcgcccgat gcaccgaccc cgaccgatgc gcaggaaccg aacgcgtacg acatcgcgct
     4681 gccgctgaat ccagcgatca ggtacaggat caggaacttc acgtgaccga acgcttgctc
     4741 cacgttgggc ccgaatagga acagggcgta ggagttgaat ccgatgtgcc agatggccac
     4801 cggagcgtgc aagaacatcg gggtcaagag tcggtaccac tcgccggcag cgatctggat
     4861 ggggatcgca ccgaaccgga acgtgaggtc tcggtagacg aaggattgag tcagccagac
     4921 gacgacgttg atcaccaaga tcgtgaacac caccggggcg gtcgcccgaa ttgccgaacg
     4981 gaccttggga gcgcttttct gcgcttcggc tacgcactcg ggacagtgct gaccaaccga
     5041 tgcagggatc gcgcaccggc cgcagatgag acggtcgcac ctcgtgcagc ggagccgcgt
     5101 gggtgtgtct ttatgtgcgt agcagtacgt catctcttcc ggctcgacgg atgtcggcgc
     5161 cgggggctcg ggagtcgtgc tcacgcaggc catgctccca tacgggtact cgtaccctcg
     5221 cggcatgccc gtccgagttg caacgatgaa cctgcaccac gccgggggta tcgacggccg
     5281 cgtcgatctc gaacgatgcg ctgaggtgct gaagtccacg cggtcggaac tattcgcact
     5341 gcaagagttg gatcgcaacc tcgagcgcag cggacgcgtg gaccaacccg ggctgctagc
     5401 cggcttgctc ggcatgccgg ttcacttcca ccccacgttg acgagaggcg agggcgagta
     5461 tgggatcgcg atcgcggcag agggtctgag tgagatctcg gaggaagatc tgccccgcct
     5521 gggtgacgaa gagcccgcgc cgctttgatg gcgaggtggc gtggattcac agtgatcgct
     5581 acccaccttt ctcccgttcg agcggctcgg cgcacacagc tggcccggct ggtcgagctc
     5641 gcacgggaac cggcggaccc cgtcctgatc ctgggcgacc tcaattgcgg ccgactcggt
     5701 ctgggctccc ttgccgccgc cgggttccgg gggcggggcc tagggcccac gaccgagaga
     5761 ggttggcccc ggcggatcga ccacgtcctt gcggggccgg gggcccgggt ggtggtggcg
     5821 aaggtgatcg ggagcggggt ctcggaccat cgagccgtgg tcgcggaagt agcccgaacc
     5881 cggtgactac tgttactggt gttgctattg ttactggtgt tactcaggag ccggactatt
     5941 gccggggagg ccgctgtggc tcatgtgacg atgcaggacg tgtccacgat ggttcgcccc
     6001 gcccgcgcgc gcttcccgtg gcggggcctg ttgatgggcg cgctggccgt caccgcggcc
     6061 gcagggatcg cgtgggcggc ggtgccccgt ccggcctcct cggaccccga tctcaccgca
     6121 gtgcgtaccc aggcagccgc cgcgaccgag gccgtggctc gcctggaggc gcaggtcgcc
     6181 gatctgaaag ccgatctgag agcgctggac aggtcgggag acagaaccgg agcacgacta
     6241 ggtaaagcga cgaccgctct gtggggctcg ctcgatcgac tgaggacctc gctcaaggat
     6301 cagcgggggg ctacgtcggc tgccaaccaa gaggcactcg cggctttgga gcgcgccgaa
     6361 agcgcggccc gcaatctctc ggttctagaa gaccgcttcg aataccacct gcgctcggat
     6421 cacggaggcg gctaagtgag gctcttacga gatctggtcg gccagactct ctccggccgg
     6481 tatcgcctcg tcgcgcgctt ggccggcggg gggatggggg aggttttccg cgggcacgac
     6541 cttctgttgg atcgccccgt tgcgatcaag atcctgcaac cctccctcgc cagcgatccc
     6601 gacctcgtcg agaggttcaa acaagaagcg cgcgccgcag cgcgcctcaa ccatcccaac
     6661 atcgtttccg tatacgactg gggtgccgag gacgatgaga cctattacat ggtgatggag
     6721 tacgtctcgg gcaccgacct ccgcgaggtc atcaccttga gggctccgct cgaacccggt
     6781 caggcggtcg acacgatgtc ggtggtttgc gacgcgctgg cggccgctca tgcctcggga
     6841 ctagtacata gagacgtt
//
LOCUS       DATMED010000387         4175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4918698, whole genome shotgun sequence.
ACCESSION   DATMED010000387 DATMED010000000
VERSION     DATMED010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4918698"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VNP73_10090"
     CDS             <1..566
                     /locus_tag="VNP73_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWL66310.1"
                     /translation="PLFEGDNDNITGLLYAKDLMKRMHESKGTTKVSSIARPPTFVPE
                     QKKVAELLREMQDARVHMAVVVDEYGGTAGLVTIEDLIEEIVGEIVDEYDQEEPLVEP
                     LDDSTIRVDAKMPIDEVNELLGVDLPHEEWDTVGGLVFGLTGRVPQVGERVRYDSLEF
                     RTERVTGRRIQKVVITKLPGEPQEASG"
     gene            578..1465
                     /gene="era"
                     /locus_tag="VNP73_10095"
     CDS             578..1465
                     /gene="era"
                     /locus_tag="VNP73_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017605119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWL66311.1"
                     /translation="MFRSGFVAIVGRPNVGKSTLVNALVGEKVAITSDKPQTTRSAIR
                     GIVDSTDAQVVLIDTPGYHKPRTLLGKRLNEVVRAAWSDVDLAVFVLDGAAGVGRGDA
                     KVAADLSAAGCPVISVVNKIDAMSPPKIAEALAQASGLGEYAEFVPISAKAVDGIDVL
                     RELIVSRMPEGPVYYPPGTRTDQPPPAFVAELVREKLLSRAHEEVPHSIAVITEDYEE
                     RDDGLLAINATIYVERDSQKGIVIGKGGSNLKEAGTEAREEMELLFGRKVFLGLRVKV
                     EKDWQRKAHALERLGFVSS"
     gene            1462..2418
                     /locus_tag="VNP73_10100"
     CDS             1462..2418
                     /locus_tag="VNP73_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL66312.1"
                     /translation="MRNAPACEVRSLRKIYRDPEVVANDGIDFSVELGTSFGLLGPNG
                     AGKTTLVRQLVGLMRPTAGKVLLFGEEVQTGRSSQSVGRHMAYLPQGAFSLGELKVRE
                     ALQYTAMLRGCGRATARSESESLMEVLELGPLADRQLRKLSGGQRRLTQVAMTLVGRL
                     PVLILDEPTTDIDPELRGRIWTLLAERAGDGAAVILVTHDVAEAEHVLDRVAILHEGR
                     IVAAGRPAELKARLAHRTRIEVVIAEGTTLGDSFLTLLGPDHRVERRRVSAWVAADEA
                     VMTLEKVVSAAGPGALEDVRLITPTLEDVYLELGGHHLKDEE"
     gene            2415..3158
                     /locus_tag="VNP73_10105"
     CDS             2415..3158
                     /locus_tag="VNP73_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL66313.1"
                     /translation="MNSTSFGKFTVLLRMQLTRLRTSWRPYLIVSSVMPLGIALLMRA
                     VMDRGQVDRFGIQIVAGSAVLAVSMTAVVMLAQRISWLKETQALDYYATVPVSRSSVV
                     AAVLISFAVFSLPGAILVVGVGALMFDLSLTALWTVVPVWALGSFALAGLGILIGFTA
                     RDEQLAGMYSNLVMMAVLFLGILPAAKLPGWVAPLRAVLPSTYAVDALKPGLAGSFGA
                     GQVVDLLVLAVFGAIFFACVRGPLWPKGE"
     gene            3160..3369
                     /locus_tag="VNP73_10110"
     CDS             3160..3369
                     /locus_tag="VNP73_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66314.1"
                     /translation="MPQATVKSYDSITRTGVLISDDGSTEYPVEGESLDTGMFRFLRP
                     GQRVTFDLVDVGGEKRLRNLRIGIA"
     gene            3549..4028
                     /locus_tag="VNP73_10115"
     CDS             3549..4028
                     /locus_tag="VNP73_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3804..3806,aa:Sec)
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
                     /protein_id="HWL66315.1"
                     /translation="MANPFSGVPTSFRVTSGERSWWGNCIWDGLGILTMLDVDGRVET
                     SCPDCGDVLALERVGSELRGDGGVAHFSVPAARWWDDIGFTUATMLFFRSEEHVDRWV
                     DAGHPRGEVFTLDQLWALARDWYSNRLDPDWKRFSTDQAHAVFAGVGLTGDFWRLDA"
BASE COUNT          719 a         1230 c         1431 g          795 t
ORIGIN      
        1 ttcctctctt cgaaggagac aacgacaaca tcacaggttt gctctacgcg aaagacctga
       61 tgaagcggat gcacgagtcg aaagggacga cgaaggtctc ttccatagcg cgacccccga
      121 cgttcgttcc cgaacagaag aaggtggccg agctgctgcg cgagatgcaa gacgcgaggg
      181 tccacatggc tgtggtcgta gacgaatacg gaggcacggc cggactcgtg acgatcgagg
      241 acttgatcga ggagattgtc ggcgagatcg tcgacgagta cgaccaggag gaaccgctgg
      301 tagaaccact cgacgacagc actatccgcg tcgacgcgaa gatgccgatc gacgaagtca
      361 acgagttgct cggagtcgat ctcccgcacg aggaatggga cacggtcggg ggcctggttt
      421 tcgggttgac cgggcgggtt cctcaagtgg gggagcgcgt tcgttacgac tcactcgagt
      481 ttcgtaccga gcgcgtgacc ggacgccgga tccagaaggt cgtgatcacg aaacttcccg
      541 gcgagcctca agaagcgtcc gggtaagggg gcgcgaggtg ttccgctccg ggttcgtcgc
      601 catcgtgggg cggcccaacg tcggcaagtc aacgctggtg aacgcgctcg tgggcgagaa
      661 ggttgcgatc acttcggaca agccccagac cacgaggagc gcgatccgag ggatcgtgga
      721 ctcgaccgac gcacaggtcg tgttgatcga cacgcccggc tatcacaaac ctcgcacgct
      781 gctgggcaag aggttgaacg aagtcgtgcg tgcggcctgg tccgacgtgg acctagccgt
      841 gttcgtcctc gacggcgcgg cgggagtggg ccgtggtgat gcgaaggtgg ctgcggatct
      901 gagcgcggcc ggttgcccgg tcatctcggt ggtcaacaag atcgacgcga tgtccccgcc
      961 gaagatcgcc gaagccttgg cgcaggcgag cggactgggc gagtacgccg aattcgtgcc
     1021 gatctcggcc aaggccgtcg acggcatcga tgtcctccgc gagctgatcg tctcgcgcat
     1081 gccggagggc ccggtgtact accctcctgg tacacgcacg gatcaacctc cgccggcatt
     1141 cgtagcggag ctggtgcgtg agaagctcct ctccagagct cacgaagagg ttcctcactc
     1201 gatcgccgtg ataacggagg actacgagga gcgggacgac ggcctactgg cgatcaacgc
     1261 gaccatctac gtcgaacgag actcgcagaa gggcatcgtg atcggcaagg ggggcagcaa
     1321 cctgaaggaa gccggtaccg aggcgcgcga ggagatggag cttctgttcg gccgaaaggt
     1381 gttcctcgga ttgagggtga aggtggaaaa ggactggcag cgcaaggctc atgcactcga
     1441 gcgactgggg ttcgtgtcct catgaggaac gctcccgcgt gcgaggttcg ctcactgcga
     1501 aagatctatc gagatcccga ggtcgtggcc aacgatggga tcgacttctc ggtcgaacta
     1561 ggcacgtcgt tcggcttgtt gggcccgaac ggtgccggga agacgacgct cgtccggcag
     1621 ctcgtcgggc tgatgcgtcc gaccgcgggc aaggttctcc tcttcggtga agaggtgcag
     1681 accggtcgtt cctctcagag cgtcggtcgg catatggctt acctccccca aggcgcgttc
     1741 tcgctcggcg agctcaaggt gcgagaagca ctgcagtaca cggccatgct ccgtgggtgt
     1801 ggacgcgcga ccgcgcgatc ggaaagtgag agcctcatgg aggtgctcga gctcggcccc
     1861 ctcgcggacc gtcagcttcg gaaactgtcg ggcggtcagc gccgcctcac acaggtggcg
     1921 atgactctcg tcggtcgtct accggtgttg atcctcgacg agccgaccac cgacatcgac
     1981 ccggagctcc ggggccggat ctggaccctg ttggcggaac gcgccggcga cggggcggcc
     2041 gtcatcctgg tgacccacga tgttgccgag gccgaacacg tcctcgaccg ggtagcgatc
     2101 ctgcacgagg gccgcatcgt tgcagcgggt cgtcctgcag agctcaaagc tcgactcgct
     2161 caccgaacgc ggatcgaggt agtcatcgcg gagggcacga cactcggcga ctcattcctc
     2221 acattgctgg gccccgatca ccgcgtcgag cgcagacgcg tcagcgcatg ggtcgctgca
     2281 gacgaagcgg tgatgacctt ggagaaggtc gtttctgcgg ccggacccgg cgctctcgag
     2341 gatgtcaggc tcataactcc caccctcgag gacgtctacc tcgaactcgg cggccatcac
     2401 ttaaaggacg aagagtgaac tcaacgtctt tcggcaagtt cacggtgttg ctgcgcatgc
     2461 agctcacgcg cctgaggacg tcgtggaggc cgtacctgat cgtttcatcg gtgatgccgc
     2521 tcggcatcgc gttgttgatg agggccgtca tggaccgagg tcaggtcgat cgtttcggga
     2581 tccagatcgt cgcgggttcc gcggtgctgg cagtctcgat gacggcggtg gtgatgctgg
     2641 cccaacgcat ctcgtggctc aaggaaaccc aggccctcga ttactacgcg acggttccgg
     2701 tgtcgcgctc gagtgttgtt gcggccgtgc tcatctcgtt cgcggtgttc tccttgcccg
     2761 gcgcgatcct cgtcgtcggg gtgggtgcgc tgatgtttga tctctccctg accgcgttgt
     2821 ggacggtcgt tcccgtatgg gctttgggat cgttcgctct cgcaggactc ggcatcctga
     2881 tcgggttcac ggcgcgcgac gagcaactcg ccggcatgta ttcgaacctg gtgatgatgg
     2941 ccgtcctctt cctggggatc ctgccggccg cgaagctccc cgggtgggtc gctccgctta
     3001 gggccgtctt gccgtcgacc tatgccgtgg atgcgttgaa gccggggctc gcggggtcgt
     3061 tcggcgcggg tcaggtcgtc gacctcttgg ttctggcggt cttcggggcg atattcttcg
     3121 cctgcgttcg agggccgttg tggccgaaag gcgagtgaga tgccgcaagc aacagttaag
     3181 agttacgact ccatcacgcg caccggcgtg ctgatctcgg acgacggctc gaccgagtac
     3241 ccggtcgagg gtgaatcgct cgataccggc atgttccgtt tcctgcgacc cggccagagg
     3301 gtcacgttcg atctcgtcga cgtgggcggt gaaaagaggc tccgcaacct ccgcatcggc
     3361 atcgcttgac gctcgacgcg tacgttcgat cattcatcct tcgcaccttc gtcgaccacg
     3421 ggcgccctcc gtctttagag gagatcgcgg cggagtgcga tatgcccgtt ccgaaagtga
     3481 agcccgtcct cgagcggctg gccaacgcgc acgtggtcaa cctcgttccc ggctcccact
     3541 acatcttgat ggcgaatccg ttctcgggcg tgcccacgtc gttccgggtg acgtcggggg
     3601 agcggtcgtg gtggggcaac tgcatctggg atgggctcgg gatcctgacg atgctcgatg
     3661 tcgacggtcg cgtggagacg tcgtgccccg actgtggtga cgtcctcgct ctagagagag
     3721 tcggcagcga gttgcgtggc gacggagggg tggcgcactt ctcggtcccc gccgctcgct
     3781 ggtgggacga catcgggttc acctgagcaa cgatgctgtt cttccggtcg gaggagcacg
     3841 tggaccgctg ggtcgatgcc ggacacccgc gcggtgaggt cttcaccctc gatcagttgt
     3901 gggcgctcgc tcgggactgg tattcgaacc ggttggatcc ggactggaag aggttctcca
     3961 cggatcaggc ccacgcggtc tttgccggcg tgggcctgac gggagacttc tggcgcctgg
     4021 acgcctaaag gcctagtagt agcactccat gaggttgccc tgctggtcga tgtaagagcc
     4081 gttcgggtcg atgtgcacgc tgggggggtt gagatggcac gcgccgtggc agggatagcg
     4141 atcgcaggca gctgccgggg tggccgaagt gatcg
//
LOCUS       DATMED010000388         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3003151, whole genome shotgun sequence.
ACCESSION   DATMED010000388 DATMED010000000
VERSION     DATMED010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003151"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VNP73_10120"
     CDS             <1..653
                     /locus_tag="VNP73_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66316.1"
                     /translation="GETPTWPEGKRFAAHHALLDGDIAYLGYGDAGLVVLDIADVGSP
                     KKIGHLQWSPGGDTHTCLPLPGRKLVVATDEATSERCAEESHYVRVIDVSEPADPRVV
                     SICPQPEGDYCERGMRFGPHNLHENRPGTYSSETIVFVTYFNAGLRVYDLSKAGAPTE
                     IAFWEPATPAGQEAPQINDLLVDDDLNIFVTDRITGGLYVLRPDEVLTRTMTEGKL"
     gene            650..1669
                     /locus_tag="VNP73_10125"
     CDS             650..1669
                     /locus_tag="VNP73_10125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWL66317.1"
                     /translation="MTEKRIDLHCHFIPPALIDRIAGDGGAHSITISDDERVSFAGKR
                     STQKLPRGMRDLTDRLERMDEQGVDLQIISAWMDFAGLELHAEDGAWLARGLNELTME
                     AIASHGDRFRAMAAVPLQAPELAAEELRYALEELGMVAVEIATTLGASELDDPGLAPF
                     WTAAEELDALVLVHPMNQSIGYERLSRSYFLDNIVSNPAEGAVAAANLIFGGVLVKHP
                     RLKICLTHGGGFLPYQIGRLDRGYAARPQVTAVNLDSPPSAFLRRFYYDTVLHSPEAV
                     RFLVERVGADRVVLGSDYPFPMGDPEPVGTVLKSGLDDATTAGLLGGNAIHALAPHLD
                     GLRDR"
     gene            1708..1989
                     /locus_tag="VNP73_10130"
     CDS             1708..1989
                     /locus_tag="VNP73_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HWL66318.1"
                     /translation="MSHSFLIHDDADDVGVAVTDLEASQKVTGKYMNSGKTVGLTSRD
                     AVPLGHKIALRDLADGADVVKYGVRIGRTVTAVREGDYVHTHNLKSARW"
     gene            1983..>2134
                     /locus_tag="VNP73_10135"
     CDS             1983..>2134
                     /locus_tag="VNP73_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HWL66319.1"
                     /translation="MVAGPTGYRRPDGRVGVRNQVAILPVDDISNTAAEMVAAMIPGT
                     LALPHA"
BASE COUNT          393 a          696 c          671 g          374 t
ORIGIN      
        1 gtggcgagac gcccacctgg cccgagggca agcgtttcgc cgcgcatcac gctctcctcg
       61 acggcgacat cgcctacctt ggatacggag acgccggcct tgttgttctc gacatcgccg
      121 acgtcggctc gcccaagaag atcggacacc tgcaatggag tcccggcggt gacacccaca
      181 cctgtcttcc tcttcccgga cgcaagctcg tggtcgcgac agatgaggcc acgagcgagc
      241 gctgtgcaga ggagagccac tacgtacgag tgatagacgt ctccgagccg gccgacccgc
      301 gcgtcgtgag catctgtcca cagcccgagg gcgattactg cgagcgcggc atgcgcttcg
      361 gcccccacaa cctgcacgag aacagaccgg gaacgtattc cagcgagacc atcgtcttcg
      421 tcacctattt caacgcgggc ctaagggttt acgacctctc caaagcgggg gctccgacgg
      481 agatcgcatt ctgggagccg gcgacgccgg cggggcagga ggccccgcag atcaacgacc
      541 tgctggtcga cgacgatctg aacatcttcg tcactgatcg catcaccggc gggctctacg
      601 tgctacgtcc cgacgaagtg ctgacgcgca ctatgaccga ggggaagctg tgaccgaaaa
      661 gcgcatcgac ctccactgtc acttcatacc gcccgccctc atcgaccgca tcgcgggcga
      721 cgggggagcg cactcgatca ccatctccga cgacgagcgc gtttcgttcg caggcaagcg
      781 ctcaactcag aagttgcctc gtggaatgcg cgacctcacc gatcgcctcg agcgcatgga
      841 tgagcaggga gtggatctgc agatcatctc ggcctggatg gacttcgccg gcctcgagct
      901 ccatgcagag gatggtgcct ggctggcgcg cggcttgaac gagttgacga tggaggcgat
      961 cgcttctcac ggcgaccgct ttcgtgccat ggctgccgtg cccttgcagg cgccagagct
     1021 cgcggccgag gagcttcgct acgcgctgga agagctcggc atggttgccg tcgagatcgc
     1081 gacgacgctc ggcgcttccg agttggacga tccgggtcta gctccgttct ggactgcggc
     1141 cgaggagctc gacgcgctcg tcctcgttca tcccatgaac caatcgatcg gttacgaacg
     1201 attgagccgc agctactttc tcgacaacat cgtcagcaac ccggccgagg gcgcagtcgc
     1261 cgccgccaac ctgatcttcg gtggcgtgct ggtcaagcac ccgagactca agatctgcct
     1321 gactcacggt ggggggttcc ttccgtatca gatcggcagg ctggaccgtg gctacgcggc
     1381 gaggccccag gtcacggcag tcaacctcga ctctccaccg tcggcgttcc tgcgccgctt
     1441 ctactacgac accgtcctgc attcgcccga ggccgtgcgc ttcctcgtcg agagggtcgg
     1501 cgccgacagg gtggtgctcg ggagcgacta tcccttcccg atgggcgatc cggagcccgt
     1561 cggcacggta ttgaagagcg gtctggacga cgcgacgact gccggacttc tcggaggcaa
     1621 tgcgattcac gcgctcgctc cccatctcga tggcctgcgc gaccggtgag atcgcccgtg
     1681 ggtgagctga gcagataggg ggtgtggatg agtcattctt tcttgattca cgatgacgcc
     1741 gatgatgtcg gcgtggccgt aaccgatctg gaggcgagcc agaaagtcac aggcaagtac
     1801 atgaacagcg gcaagacggt ggggctgaca agccgagatg cagttccact gggccacaag
     1861 atcgctttaa gggatctcgc ggacggcgcc gacgtcgtca agtacggggt tcgaatcggc
     1921 cggaccgtga ccgccgttcg ggaaggcgat tacgttcaca cccacaacct gaaatccgcc
     1981 cgatggtagc ggggcccacc ggctaccgac ggccggatgg ccgcgtcggg gtgcgcaacc
     2041 aagttgcgat cctgcccgtc gacgacatct cgaacaccgc cgccgaaatg gttgcggcga
     2101 tgatccccgg cactctcgcg cttccccacg ccta
//
LOCUS       DATMED010000389         8429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1064075, whole genome shotgun sequence.
ACCESSION   DATMED010000389 DATMED010000000
VERSION     DATMED010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064075"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VNP73_10140"
     CDS             <1..938
                     /locus_tag="VNP73_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWL66320.1"
                     /translation="AAEWGFDGLELACWGDHFEVTKALEEDDYVQRKRDLLDKFDLRC
                     FAISNHLVGQAVCDFPIDERHRAIVPEHVWGDGDPAGVRSRAAEEMKITARAAARLGV
                     KVVNGFTGSSIWHLLAGFPPVPPEMIDDGYRDFADRWNPIIDVFDEVGVRFALEVHPS
                     EIAYDFWTTKRTLEAIDNREGFGLNFDPSHMNWQFLDPIAFITEFADRIYHVHAKESE
                     RNLDGRNGVLASHLPFGDIRRGWDFVSVGHGSVPFERIMRTLNAIGYSGPISVEWEDS
                     GMQREHGAPESLQHLRTLNFDPAEGAFDAAFSSDN"
     gene            complement(943..3804)
                     /locus_tag="VNP73_10145"
     CDS             complement(943..3804)
                     /locus_tag="VNP73_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL66321.1"
                     /translation="MGGSSARRSYNSFGVNSRSVTARVLGAWIKGAVRRPVRALVVIL
                     TLLLMTVGMSGALVAGDMLDRLFVADAQAQWGEVDVEVTSGESGIFEESVARRIGTVA
                     GTESSAWAPRLMLRSAVASGSSREPDASVWGLGAEEQSFAPLSAVQGTGDVLQLGPDK
                     VMINERLSERLDVGVGAPLTMVLGVPEVWLDQPGTDTPLRLPAEAVVFTATISGVVAD
                     SGVADLGRTPNVLLRRDVLQNVIDFKGYVTHLHLAAEGDADDLIRTIGPQLRLDDLVA
                     APVAEDALEIADDEGGQFRSILLTLAVLVVAAATVAATQMLIGLAEDRSREIAVLRAL
                     GVPGRAIVRLVTAESLVYAVIAVAIGLILALPVAEFFAARLSDHFASLSAGRGREQVA
                     LRPVVDAGTLIAGVIVVAVAAALAGRSAGRRLAAVDVDELLRGPLVRLPQPPLTARRP
                     IVVAMLGSLLLGAGLLGGDASDALRYIGLTLMLTAWWLHRRRLTNDRTRLDRRVAVLA
                     LVWATLGAGALADFSQGYETGFAILVVAGIVTTVAVTVLLLGRFRAVVGWIRAYAPRG
                     HWQASLRTAGAYADAASGKSGRLVATFGIVLFVAVSLEVLGSATAIDVDRQSGGFDVV
                     AESTVGMETLDVGEIEGLGAGAAVPSSTVPEDRFGTASPDDEDEDIVRLRYPVRMIAV
                     TPAFVTTQQFRLADGAPRYESAAAALDAVMADRNKAVVDRYSRPPGAAIGDDVVIDLG
                     LGPRRYELVGVLDTFLVGGVLVAPEEYIDLTASTGSTLLFGAAADDTTPKALAENVSV
                     WGRGVGVNARTMEDVAADVVSANRTFTDTFALMLLLALIVTLVAVAAVLVRSARERRP
                     YLGVLRAIGLQRGTVAAALAAEPITVALVGGVAGLLGGLVVLRLLFAVGFSDLAFVVD
                     WARVLVVLGGLAVVLVILCTLSAWPLVPRDPSEALRELA"
     gene            complement(3759..5270)
                     /locus_tag="VNP73_10150"
     CDS             complement(3759..5270)
                     /locus_tag="VNP73_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HWL66322.1"
                     /translation="MKLDRRTFLHRVAVGLGAIAATRVAGFERVLAVTRRFTKPLPIP
                     KTLTGSRITLVAREAKVQIRPGPATRMWTFNGTFPGPTIRRQAGRRTSLTIVNDLPPE
                     AKTLTIHHHGSHSASKHDGLPLPEVAIPPGGSRTYVYEHMEDGKPERAALQWYHDHTH
                     HRASFNSWMGLGGLFILEDEIESRLDLPKARYELPLFLTDRRFHENNQLDTQAFEIAA
                     ANREVGGDSFLVNGAVRPFADVEPRRYRLRVHNGSGFRIYNLVLRSRDGDVQMTQIGT
                     ESGLLPAPVRRKEVLLGPAERADLIVDFTKFAGRNLTLRSSRWANATGSDGALNALFL
                     QFRVGTEVRGRDTSRIPKRLRALPGWVSEAPEQPDRVWTFGTGIDPTTGEQAHTVNGR
                     AFDHERVDAQVELDSVETWLLVNTTSRTHLIHIHDVDWVMLDRNGAAPPAYEAGLKET
                     FRLDPGESIAVAGKFSDHIGRFMIHCHMLDHEDGGMMAAWEVVAPGAATTRSG"
     gene            complement(5267..5956)
                     /locus_tag="VNP73_10155"
     CDS             complement(5267..5956)
                     /locus_tag="VNP73_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL66323.1"
                     /translation="MTNASQDRVTLVVARALQKRYGTGTAAVTALNGVDVEITAGELL
                     AITGRSGSGKTTLLHCLSGITTPDAGTVTFDGIDLTTAGERMRTELRARRMAFVFQQL
                     NLLPALTVAENVELPLVLRGEDAASIRAASTSVLEQVGLEGRKGTLPGDLSGGEQQRV
                     AVARALIGRPDIIWADEPTGALDTATSSDVMKLLRGAVDTGRSVVVVSHAPDVADVAD
                     RVLKMRDGVIG"
     gene            complement(5966..7813)
                     /locus_tag="VNP73_10160"
     CDS             complement(5966..7813)
                     /locus_tag="VNP73_10160"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWL66324.1"
                     /translation="MFKIRRAIPLAMISMIVVSLALPAAPAGAQTPPEESDLSYEVLA
                     DGTEEPLSLEVAADGRVIWAERDGTIQVLTPEGLRIVAGELRPAGNLCASCEPEADYL
                     SHPPNRLGVGGQEEGGLHGLLLHRNFAENNTIFAYRSVAGTRKEVAPGLFWGEFHLST
                     FELDPQTNLLDMSSEKVILKVPAEWDYCCHYGGDLDYLPDGTITLTTGDDVDATSSGG
                     YGPRDTSAPWLNGELTAANPADLRGKILRFNEDGSVPNGKQPGVRSNPFVGMEGYNPY
                     IKDSPKNVYVGKAAGKPGDGWIKFDPYVYAMGFKQPWRAAVHPKTGTLYVSDVGPDAG
                     ADDPKRGPRGFEEISRVPYGGGTHYGWPRCIGPNWSYMDVDWKKMKTHGKLDCSGSAP
                     IARPIGSNKTTVRGMTGASMYYSGGLCDGSENKTTTYDCDRWPIVGSGGKTSEPVAFY
                     PAATKGPLRLPKRYNDRLFVLEHARSFILSIGSDPAGNLNLNNKDMWLVTPPKYSVNP
                     DASNPQGIVSAERGQFLSPVDGDVGPDGAFYFLEYGAFFYSPGGRVARIKCAGCMPSD
                     QSMNYGLDSQPVHAGFVPLEGTTSFPIVPIVAAIAALGIGIVRRRRLVV"
     gene            complement(7848..8246)
                     /locus_tag="VNP73_10165"
     CDS             complement(7848..8246)
                     /locus_tag="VNP73_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66325.1"
                     /translation="MARFLRSRKAAVSALLFVPALLGPSSVAAEERHVTPALAVALPN
                     GTTTGFAPNVLAISQGGSLNLLGADTQAHNIACVKFSRKTKRPLCQSEYAAAGDVKPV
                     AGVEKLREGTYSLICQAHPQMKVDLTVVGP"
BASE COUNT         1556 a         2786 c         2626 g         1461 t
ORIGIN      
        1 aggctgcgga gtggggcttc gacggcttgg agctcgcctg ctggggcgat cacttcgaag
       61 tcacgaaggc cttggaggaa gacgactacg tccagcgcaa gcgcgatcta ctcgacaagt
      121 tcgatctgcg ctgcttcgcg atcagcaacc atctggtcgg ccaagccgtg tgcgacttcc
      181 cgatcgacga acggcaccgg gcgatcgtcc ccgagcatgt gtggggcgac ggcgaccctg
      241 ccggtgttcg ttcccgggca gccgaggaga tgaagatcac cgcgcgtgcg gctgctcgtc
      301 tcggcgtgaa ggttgtcaac ggtttcaccg ggtcgagcat ctggcaccta ctggccggtt
      361 tccctcccgt tccccccgag atgatcgacg acggctaccg cgacttcgcc gatcggtgga
      421 acccgatcat cgacgtattc gacgaggtcg gtgtcaggtt cgcgctcgag gtccatccct
      481 ccgagatcgc ctacgacttc tggacaacta aacgaaccct ggaggcgatc gataacagag
      541 aaggtttcgg gctgaacttc gacccgagcc atatgaactg gcagttcctc gatccgatcg
      601 ccttcatcac cgagttcgcg gaccgcatct accacgtgca cgctaaggag tcagagcgaa
      661 acctcgacgg ccgtaacggc gtgctggcga gccaccttcc tttcggggac atccgacggg
      721 gctgggactt cgtctccgtc ggccacggat cggtaccttt cgaacgcatc atgcgcacgt
      781 tgaacgcgat cgggtactcg gggccgatct cggtcgagtg ggaagactcc gggatgcagc
      841 gtgagcacgg cgctccggaa tccctacagc acttgcggac tctcaatttc gatcccgccg
      901 aaggcgcgtt cgacgccgcg ttctcgagcg ataactgagt cgctaggcca actcccgcaa
      961 cgcctcgctc ggatcgcgcg gaacgagtgg ccacgccgat agcgtgcaca ggatcacgag
     1021 cacgacggcc aagccaccga ggaccaccaa cactcgagcc cagtcgacga cgaacgcaag
     1081 atcgctgaag cccaccgcga acaacagccg gaggacgacc agcccgccca acaagccggc
     1141 gacgcctccg acaagagcaa ccgtgatcgg ctcggctgct agtgcagcag ctaccgtgcc
     1201 tcgttgtaac ccgatcgccc tgagcacccc gagatagggc ctgcgttcgc gggccgaccg
     1261 caccagaacc gcggcgacgg caaccagcgt cacgatcagt gccagcagga gcatgagagc
     1321 gaaggtgtcg gtgaaggtcc ggttggccga cacgacgtcc gccgccacgt cctccatggt
     1381 ccgtgcattc accccaacgc cacgccccca cacgctgacg ttttcggcga gggccttcgg
     1441 cgtcgtgtcg tcggcagcgg ctccgaatag caacgtagaa cccgtggagg ccgtgaggtc
     1501 tatgtattcc tcgggggcga ccagcacgcc gccgaccaag aacgtgtcca agacccccac
     1561 gagctcgtac cgtcgcgggc ctaaaccgag gtcgatgacg acatcgtcgc cgatcgctgc
     1621 tcccgggggc cgcgagtaac ggtcgacgac ggccttgttt cgatcggcca tcacggcatc
     1681 gagcgcggca gcagcagact cgtacctggg tgctccatcg gcgaggcgga actgttgggt
     1741 ggtcacgaac gccggtgtga ctgcgatcat ccgcaccggg tagcggagcc ggacgatgtc
     1801 ctcgtcctcg tcgtccgggg aggcggtacc gaagcgatct tccggcaccg tgctggatgg
     1861 aacggcagcg cccgccccca acccttcgat ctcgcccaca tcgagcgtct ccatacctac
     1921 cgtcgactcc gccacgacgt cgaatccacc ggactggcga tccacgtcga tcgcggtcgc
     1981 gctacccaac acctccagcg acactgccac gaagagcacg atgccgaagg tggccaccaa
     2041 ccgacccgac tttccgctcg cggcatccgc ataggcaccg gcggtacgca gactggcttg
     2101 ccaatgcccg cggggcgcgt aggcgcggat ccaaccgacg accgcgcgga atcgtcccag
     2161 gagcagcacg gtcaccgcca ctgtggtgac gattccggca acgacgagga tcgcgaagcc
     2221 ggtttcgtaa ccctgggaga agtcagccag tgcgccggcc cccagcgttg cccacaccag
     2281 cgcgagcacg gcaacacggc gatcgagccg cgttcgatcg ttcgtcaggc gccggcgatg
     2341 aagccaccaa gctgtgagca tcaaagtcaa tccgatgtag cgcagtgcat cggatgcgtc
     2401 gcctcccagc aagcccgcgc cgagaagcag gctcccgagc atcgcgacga cgatgggacg
     2461 tcgagcagtt agtggcggct gcggtaaccg gacgaggggg ccgcgtaaca actcgtccac
     2521 gtctaccgct gcaagccttc ggccggcgga tcgcccggca agtgccgcag ccaccgcgac
     2581 aactatcact ccggcgatca aggtgccggc atcgacgacg ggccgcagag cgacctgttc
     2641 gcggccccgt cctgcgctca gtgaggcgaa gtgatccgac aatcgagccg cgaaaaactc
     2701 cgcaacgggc aacgccagga tcaacccgat cgcgacggcg ataaccgcgt agaccaaaga
     2761 ctccgcagtc accagccgca ctatcgctcg acccggcacg ccgagtgctc gcaacactgc
     2821 gatctcccgc gaccggtctt ccgccagccc gataagcatc tgcgttgccg ccacggtcgc
     2881 tgctgcgacg acgaggacgg caagggtcaa gaggatgctg cggaactgtc cgccttcgtc
     2941 gtcggcgatc tcgagggcat cctcggccac cggggctgca acgaggtcgt ccaaccgcaa
     3001 ctgcgggccg atcgttcgga tcaggtcgtc ggcatcgccc tctgcagcca ggtgcaggtg
     3061 cgtgacgtag cccttgaagt cgatgacgtt ctgcaagacg tcgcgccgca acagcacgtt
     3121 cggcgtgcgg ccgagatcgg caaccccgga gtcggcaacg acgcccgaga tggtggcggt
     3181 gaataccacc gcctcggccg gcagtcgcag cggcgtgtcg gtgcccggct gatccagcca
     3241 cacctcgggc acgcccagga ccatcgtcag gggtgcgccc actccaacgt caaggcgttc
     3301 gctcagccgt tcgttgatca tcaccttgtc gggcccgagt tgtaagacgt ccccggtgcc
     3361 ctgcacggca ctaagtggag cgaagctttg ctcttcggca cccaatcccc acacacttgc
     3421 atcgggctct cgactcgacc ccgacgcgac ggcggagcga agcatcaacc gaggtgccca
     3481 agcggagctt tctgttccgg cgaccgtgcc gatcctgcga gcgacgctct cttcgaagat
     3541 cccgctctcg ccactggtta cttcgacgtc cacttcaccc cattgagcct gggcgtcggc
     3601 gacgaacagc cggtcgagca tgtcgcctgc gaccaaagcc cccgacatcc cgacggtcat
     3661 caggagcagc gtcaggatga cgacaagggc gcggaccggc ctgcgcacgg cccccttgat
     3721 ccatgcgccg agcactcgag ccgtcaccga ccggctgttc accccgaacg agttgtagct
     3781 gcgccgggcg ctactacctc ccacgcggcc atcatcccgc cgtcctcatg atcgagcatg
     3841 tggcagtgga tcatgaagcg gccgatgtgg tcggagaact tccccgccac cgcgatggac
     3901 tcgccgggat ccagcctgaa cgtttccttc aaccctgcct cgtaggccgg gggggccgcg
     3961 ccgttccggt ccaacatgac ccagtcaacg tcgtggatgt ggatgagatg cgtccgagag
     4021 gtggtgttaa cgagaagcca tgtttccaca gaatcgagtt cgacctgtgc gtccacacgc
     4081 tcgtggtcga acgcgcgacc gttcacggtg tgtgcctgct cccccgtcgt tggatcgatc
     4141 ccggtcccga acgtccacac acgatcgggt tgctcgggcg cctcgctcac ccaacccggc
     4201 aacgcccgca gtcgtttagg gatcctggat gtatcccttc cacgcacttc ggtgccgact
     4261 cggaactgaa ggaacagcgc gttgagagcg ccgtccgagc cggtggcatt ggcccagcgc
     4321 gacgatctga gcgtgaggtt ccgaccggcg aacttggtga aatcgacgat caggtcggcg
     4381 cgttccgccg gtcccagcag cacctccttg cgtcgaacgg gcgcgggcag gagaccactt
     4441 tcggtgccga tctgtgtcat ctggacgtcc ccgtcacgag agcgcaagac aaggttgtag
     4501 atgcggaagc ccgagccgtt gtgaacccgg agacggtaac gacgaggctc gacgtcggcg
     4561 aaaggacgta cggcaccgtt taccaagaag ctgtcccctc caacctcacg gttggcagcg
     4621 gcgatctcga aagcctgcgt gtcgagttgg ttgttctcat ggaaccggcg atccgtaagg
     4681 aacagcggga gctcgtaccg agccttcggc agatccaggc gcgactcgat ctcgtcctcg
     4741 aggatgaata ggccaccgag gcccatccac gagttaaagc tcgcccggtg atgggtgtgg
     4801 tcgtggtacc actgaagcgc cgcgcgctcg ggtttcccat cctccatgtg ttcgtacaca
     4861 taggtgcgcg agccgccggg tgggatagcg acctccggca acgggaggcc gtcgtgtttc
     4921 gaggccgaat gtgaaccgtg atggtggatg gtcaacgtct tcgcttcggg tggcaagtcg
     4981 ttcacgatgg tcaaactcgt ccgcctgccg gcctgcctac ggatcgtcgg tcccgggaag
     5041 gtgccgttga acgtccacat ccgtgtggcc gggccgggcc ggatctggac ctttgcctcg
     5101 cgcgcgacca acgtgatccg agatccggtg agggtcttcg gtatcggcag gggcttggtg
     5161 aaccgccttg tgacggcaag gacacgctcg aaaccggcaa ctcgcgttgc cgcgatcgct
     5221 ccgagcccta ctgccactcg gtgaaggaaa gttctccggt ccagcttcat ccgataacac
     5281 cgtcgcgcat cttcaggact cgatcggcca cgtcggcgac gtccggagcg tgggagacga
     5341 caaccaccga gcgaccggtg tcgacggcgc cgcgcaggag cttcatgacg tccgaggacg
     5401 tggccgtatc cagagcaccg gtcggttcgt cggcccagat gatgtcgggc cgtccgatca
     5461 gtgcacgggc aacggcaacc cgctgctgct ctccacccga taggtccccc ggcagcgttc
     5521 ccttccggcc ctctagaccc acctgctcga gcacggaagt cgaagcggcg cggatcgaag
     5581 cagcatcctc tccgcgcagt acgagcggca gctctacgtt ctcggcgacg gtcagcgccg
     5641 gtagcaggtt gagctgttgg aacacgaacg ccatccgtcg cgctcgaagt tccgtacgca
     5701 tccgctcgcc cgcggtcgtc agatcgatcc cgtcgaacgt gaccgtgccg gcgtcggggg
     5761 ttgtgatgcc agacagacag tgcaacagcg tcgtctttcc acttccggag cgtcccgtga
     5821 tggcgaggag ctcgcctgca gtgatctcga cgtcgacacc gttaagcgcg gtgaccgccg
     5881 ccgttccggt gccgtatcgc ttctgcaatg ccctcgcaac gaccaaggtc accctgtcct
     5941 gcgaggcgtt ggtcacgccc agagcctaga caaccaaacg acggcgcctg acgataccga
     6001 tcccgagggc cgcgatcgcc gcaacgatcg ggacgatcgg gaacgaggtg gttccttcga
     6061 gcggcacgaa gccggcgtga accggctggg agtcgaggcc gtagttcatc gactggtcgg
     6121 atggcatgca acctgcgcac ttgattcgcg ccacgcgacc ccctggcgaa tagaagaaag
     6181 ccccgtactc gaggaagtag aaggcgccgt cgggtccaac gtcgccgtca accggagaaa
     6241 ggaactggcc tctttcggcc gacacgatgc cttggggatt gctcgcgtcc ggattaaccg
     6301 aatacttcgg cggggtgacg agccacatgt ctttgttatt gagattcagg ttccccgccg
     6361 gatcgctgcc gatgctgagg atgaagctgc gagcgtgctc gagaacgaag aggcgatcgt
     6421 tgtaacgctt cggcaaacgg aggggtccct tcgtcgccgc gggatagaag gcaacgggct
     6481 cgctggtctt gccacccgac cccacgatcg gccacctgtc acagtcgtag gtcgtcgtct
     6541 tgttctcact gccgtcgcac agaccgcccg agtaatacat cgacgcgccg gtcatgcccc
     6601 gcaccgtcgt cttgttcgaa ccgatcgggc gtgcgatcgg cgcgctcccg gagcagtcga
     6661 gcttgccgtg ggtcttcatc ttcttccagt cgacgtccat gtaggaccag ttcggcccga
     6721 tgcagcgcgg ccatccgtaa tgcgtgccgc cgccgtaggg cacccggctg atctcctcga
     6781 agccccgcgg cccccgtttg gggtcgtccg cgccggcgtc gggaccgaca tcggagacat
     6841 agagcgtgcc cgttttcggg tgcaccgccg cgcgccatgg ttgcttgaag cccatcgcgt
     6901 agacgtaagg gtcgaacttg atccatccgt cgccgggctt accggcggcc ttaccgacgt
     6961 aaacgttttt cgggctgtct ttgatgtagg ggttgtatcc ctccatgccg acgaacgggt
     7021 tcgaacgcac gcccggctgc ttgccgtttg gcacggatcc gtcctcgttg aaccgaagga
     7081 tcttgccccg caggtccgcc ggattggcgg ccgtcagttc gccgttcagc cacggcgcgc
     7141 tggtgtcgcg cgggccataa ccacccgagg atgtggcgtc gacgtcgtcc cccgtggtca
     7201 gcgtgatggt tccgtccgga aggtagtcga gatcaccgcc gtaatggcag cagtaatccc
     7261 attcggccgg caccttcagg atgactttct ccgacgacat gtcgaggagg ttcgtctgcg
     7321 gatccagctc gaacgtggag aggtggaact ccccccagaa caagcccggc gcaacttcct
     7381 tgcgggtacc cgcaaccgac cggtaggcga agatcgtgtt gttctcagcg aagttgcgat
     7441 gcagcaagag cccgtgaagg ccaccttcct cttgtcctcc aacccccaga cggttgggtg
     7501 ggtgggacag gtagtccgcc tccggctcgc aggacgcgca caggttcccg gcggggcgaa
     7561 gttcgcccgc cacgatccgc aatccctccg gagtcaacac ttggatcgtt ccatcacgct
     7621 cggcccagat gacgcgtcca tcggccgcga cctcgagcga aagcggctcc tccgtcccgt
     7681 cggcgagaac ctcgtaagag agatcgcttt cttcaggtgg cgtttgcgcg cccgcggggg
     7741 cggccggaag ggctagggac acgacgatca tcgagatcat cgccagcggg atcgctcgtc
     7801 ggatcttgaa catcacgccc tccttcgttc gagcatcccg ggatctccta aggaccgaca
     7861 acggtcagat cgactttcat ctgtgggtgg gcctgacaga taagcgaata ggtgccctcg
     7921 cggagtttct cgaccccggc cacgggtttt acatcgccgg cagcggcata ctcggattga
     7981 cacaggggcc gcttcgtctt gcgactgaac ttcacgcagg cgatgttgtg cgcctgggtg
     8041 tcggcgccta acaggttgag gctcccgccc tgggagatcg ccagcacgtt gggtgcgaag
     8101 ccggttgtgg tgccgttggg aagtgccacc gcaagagccg gtgtcacgtg tcgctcctcg
     8161 gccgcaaccg aactgggccc gagtagggca gggacgaaga gcagggcgga gacggccgcc
     8221 ttcctagagc gaagaaatcg cgccacgttg ctctcccctg aatcccgacg acgtttctct
     8281 agagggatac ggcccacgaa tccttattcc tgctaccgac gaaaaataac cacctttggc
     8341 ctatccgacc gaatactttt gccaagggat cggcttaagt gccgcctgcg gagggcctag
     8401 cctctcctta tccggatgtg gatgtggat
//
LOCUS       DATMED010000390         4431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5301208, whole genome shotgun sequence.
ACCESSION   DATMED010000390 DATMED010000000
VERSION     DATMED010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5301208"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VNP73_10170"
     CDS             complement(<1..631)
                     /locus_tag="VNP73_10170"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HWL66326.1"
                     /translation="MVAGYLLKAQCLSPWLEGHQYGSLCYNDIQPLYLDRGLESGQFP
                     YVDGAIDGTELSGGAIEYPVLTGVFMWFVATFVSHDMNAYLIATALALAPFGLLAAYW
                     LARMTGWRALMWAAAPAVILYSFHNWDLLVVAATVGGFWRWRRGDSLGAAIAFGIGGC
                     LKMYPLMFLAPLVLEQLQRRRIADAVWTGLGGTATFALINAPFAYFNLEG"
     gene            complement(709..2988)
                     /locus_tag="VNP73_10175"
     CDS             complement(709..2988)
                     /locus_tag="VNP73_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWL66327.1"
                     /translation="MSRSSRQPARTTTKRAPAKKGGSRARAKAQPRSRWQRFLLRYGW
                     TLPIGAILVGSGILGLTYAFASIPLPTELDLDSSAEVYDANDELIGTFSGEVRRFLID
                     TSKLDKFVGEAVIAAEDRDFYKHNGVSVRGIVRAAWANITDTQISQGGSTITQQYVKN
                     AVLEDPSRTLTRKLKEAVLSVKLERRYSKKQILGFYLNTIYLGRGAYGIEAAARAYFD
                     KNATELTLGEAAYFAGIIPSPESYQPDSNPQGAVARRNRVLRVMEDLGYVSERKARKA
                     ARGRVRLAKQDDHRQRQPAAYFIEWLRKVYLYPEYEECLYTCGLKIYTTLDVDMQNAA
                     EDAVDSILTEKKDPEAALVSTTPKGEIRAFVGGRSFTNVRRARGFNYASDYPGRQAGS
                     AFKPFTLLTAIEEGISPQSRFSGASPTYVPAPCAGDNGLPPWEVENYGGSSYGTLTLD
                     QATTNSVNAVYGQLIHEVGPDKVAEKLEDLEFDRSGTSARREIPENCGLSLGTLDVTP
                     VEMARAYASFAGRGVLPEIMPIRYITNSSGDCLKKYRPEQGVECEDEDRLAMERVADQ
                     NTADVLTQTLTHVVQGGTATIANIGRPVAGKTGTTQLNVNAWFAGYVPQLVTVVWEGY
                     PAEKNGELVPRMAYCSDPVECRPVHGQEVTGGNSLVGPAVIWRNFMAAATEELPAAPF
                     PLPTDIPDTIINSPPPAPPSTPEPDKSKKPKPEPTEGEPTTEPTPTQEPTPTEQPTPI
                     ITPTTGDEPRERRRREDDP"
     gene            complement(3027..>4431)
                     /locus_tag="VNP73_10180"
     CDS             complement(3027..>4431)
                     /locus_tag="VNP73_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66328.1"
                     /translation="VLALIAFVMRDAEEVRARGVQVISLVGVTLGAILFFLRHSGLKW
                     RTLDPDPGPTVAAGVACACTWLLVLSLDKGRGRWEPACLAGVGSCGLVLFALNAWAVP
                     ALLFWWTSSAALAAAARNSVGRSQFWLALAASDLLLTAGIALHALGEEIWALPGGLSG
                     MSFWFVAAAALVRAGFLPRIGLWGASDFPLWPLLPGGAFAIAGGLTGREEPWLGLAVL
                     LGALAIAVWFLVRPRSDLSFPASWPVALMLAVLFVVPGVPWQASAAGLIASTSVLLWP
                     IATGRAQIERGLLISFLPPSAGFSAIVVAAVATFDQATATTDVLSAAPWTAVTALLPA
                     ALGAGVILAIRVSRRIEAEGYEPTAVLVTWGLFFVSIVAGLWPRFVSETDGGATSRVF
                     VLHLVALGAGIAAARFVSRPAPEKVGEEVEPLVVAYAPLPALWDRTAQLAAASVGVGG
                     AALALWVTYRGLQVGFL"
BASE COUNT          848 a         1490 c         1353 g          740 t
ORIGIN      
        1 agccctcgag gttgaaatac gcgaacggcg cgttgatcaa agcgaaggtc gccgtgccac
       61 ccaacccggt ccacacggca tccgcgatcc tgcgacgctg cagctgctcc aacaccagag
      121 gcgcgaggaa catcagcgga tacatcttga ggcaaccacc gataccgaag gcgatcgcgg
      181 cccccagcga gtcaccacgt cgccaccgcc agaatcctcc gaccgtggct gcgacgacca
      241 gcaggtccca gttgtggaac gaatagagga tcactgcggg ggccgcggcc cacatcagcg
      301 cgcgccaacc ggtcatccgc gcgagccagt acgccgcgag caggccgaat ggcgcgagcg
      361 ccagagccgt cgcgatcaag tatgcgttca tgtcgtgcga cacgaacgtc gcgacgaacc
      421 acatgaagac accggtgagt accgggtatt cgatggcgcc gccggacagc tcggtcccat
      481 cgatcgcacc gtccacatag gggaactgac ccgactcgag cccgcgatcc aagtacagcg
      541 gttggatgtc gttgtagcaa aggcttccgt actgatgccc ctccaaccac ggtgacagac
      601 actgcgcctt cagcagatag ccggcgacca tggtcagcgc gcagatcgcg acgatgatcg
      661 gtacgacctg tgtcttcttc gacttcgact gttcgatctc ggcagttgct atgggtcgtc
      721 ctctcgtctg cgcctctctc ggggctcgtc ccccgtggtc ggcgtgatga tcggcgtcgg
      781 ctgttccgtc ggggtcggtt cctgtgtcgg agtgggctcg gtggtgggct cgccctccgt
      841 gggctccggc ttcggcttct tggacttatc gggctccggg gtcgacggcg gcgccggggg
      901 aggagagttg atgatcgtgt ccgggatatc ggttggaagc gggaacggcg ccgccggcag
      961 ctcttccgta gccgcggcca tgaagttccg ccagatgacc gccggcccca ccagcgagtt
     1021 tccccccgtc acctcttgcc cgtgcaccgg tcgacattca accggatccg agcaataggc
     1081 catccggggg acgagctctc cgttcttctc ggctggatat ccctcccaaa caaccgttac
     1141 caactgaggc acgtagcccg cgaaccacgc gttcacattt agctgagtgg tgcccgtctt
     1201 gccggcaacc ggacgaccga tgtttgcgat cgtcgcggtc cctccttgaa cgacatgtgt
     1261 aagcgtctgg gtcaagacgt ccgcggtgtt ctggtccgcg acgcgctcca tcgcgagccg
     1321 atcctcgtcc tcgcactcga caccctgctc gggtcgatat ttcttcaagc aatctccgga
     1381 gctgttcgtg atgtaccgga tcggcatgat ctccgggagc accccgcgtc ctgcgaacga
     1441 ggcgtacgca cgcgccatct caacaggcgt cacgtcgagc gttcccagcg acaacccgca
     1501 gttctcggga atctctcgcc gggccgacgt accacttcga tcgaattcga gatcctccaa
     1561 cttctccgcg accttgtcgg gtcccacctc gtggatcaat tgcccgtaga ccgcgttgac
     1621 cgagttcgtc gttgcttgat cgagagtgag ggtgccatac gaagaaccgc cgtaattctc
     1681 gacttcccac ggaggaagcc cgttgtcgcc ggcacaaggc gcgggcacgt acgtcgggct
     1741 ggcaccggag aaacgggatt gcggtgatat gccttcctcg atcgcggtga gcaaggtgaa
     1801 gggtttgaag gcggaacctg cctgacggcc cggataatcg ctcgcgtaat tgaaaccacg
     1861 agccctccgc acgttcgtga agctgcggcc cccgacgaag gcacggatct cacccttcgg
     1921 tgtcgttgaa acgagcgcgg cctccgggtc tttcttctcc gtcaagatcg agtcgaccgc
     1981 atcctcagcc gcgttctgca tatccacgtc gagggtcgta tagatcttga gcccgcaggt
     2041 gtagagacac tcctcgtact ccgggtacag gtagaccttc cgcaaccact cgatgaagta
     2101 agcggccggc tgccgttgcc ggtgatcgtc ctgcttcgcg aggcgcacgc ggccgcgcgc
     2161 ggccttcctc gccttgcgtt ccgagacgta accgaggtcc tccatcactc ttaggacccg
     2221 gttgcggcgc gcgacggcgc cttgaggatt cgagtccggc tgatagctct ccggcgaagg
     2281 gatgatcccg gcgaagtaag cagcttcgcc gagcgtcagc tcggtcgcat tcttgtcgaa
     2341 gtaagccctc gcagcagctt cgatcccgta cgcgccccga cccagataga tcgtgttcag
     2401 atagaagccg aggatctgct tcttcgagta acgacgttcg agcttcacgg acagaaccgc
     2461 ttccttcagc ttccgcgtca gggtgcgaga gggatcctcc agcaccgcgt tcttcacgta
     2521 ttgctgagtg atcgtcgacc caccctgcga gatctgggtg tcggtgatat tcgcccacgc
     2581 agcccggacg atgccccgga cagagacccc gttgtgcttg tagaagtccc tgtcttccgc
     2641 cgcgatgacc gcttcgccca cgaacttgtc gagtttcgac gtatcgatga ggaaccggcg
     2701 cacctccccc gagaaggtgc cgatcaattc gtcgttcgcg tcgtagacct ccgctgagga
     2761 atccaggtcg agttcggtcg ggagcgggat cgaagcgaac gcgtacgtca gccccaagat
     2821 gccgctaccg acgaggatcg ccccgatcgg aagggtccat ccgtatcgca acaggaacct
     2881 ctgccaccga ctccggggct gcgccttcgc acgcgctcgc gagccgccct tcttggccgg
     2941 agctcgcttc gttgtcgtgc gtgcgggttg gcgagacgat ctagacacgc tggaagtcta
     3001 gggtcggggt cactttacga gtggctttag agaaagccga cctgtagccc gcgatatgtc
     3061 acccacaacg cgagcgcggc ccccccaacg ccgaccgagg ccgcagcgag ttgggcagtc
     3121 cggtcccaca gtgcgggcaa cggggcgtaa gcaaccacca gcggctcgac ctcctcaccc
     3181 accttctctg gtgcgggtcg cgatacgaac cgggccgcgg cgatcccggc accgagcgcg
     3241 acgagatgca gaacgaagac acggctcgtt gcaccaccat cggtctcgct aacgaaccga
     3301 ggccacaacc ccgccactat cgacacgaag aagagccccc atgtgaccag gacggcggtg
     3361 ggttcgtagc cctcggcttc gatgcggcga ctaacccgga tggcgaggat cacaccggct
     3421 ccgagagcag caggtaggag cgctgtcacc gcggtccagg gcgcggccga caacacgtcc
     3481 gtcgtcgcgg tcgcctgatc gaaggttgcg acggcggcca ccacgatcgc ggagaacccc
     3541 gcacttggag gcaggaagga gataagcaat ccgcgttcga tctgcgcccg gccggtagcg
     3601 atcggccaca ggagaacgct ggtcgaggcg atcaatcctg cggcggatgc ctgccatggc
     3661 accccgggca cgacgaacag gacggccaac atcaacgcca ccggccacga cgcgggaaac
     3721 gacaggtccg atcgagggcg caccaagaac cacacggcga tcgccaatgc ccccaacagg
     3781 accgcgaggc cgagccatgg ttcctcgcgg ccggtgagtc cgccggcgat cgcgaatgct
     3841 ccgccgggaa gtaagggcca cagtgggaag tcgctcgcgc cccacaaccc gatgcgcggc
     3901 aaaaacccgg cgcgcaccaa ggccgcggcc gcaacgaacc agaacgacat cccgctaagg
     3961 cccccgggca gggcccagat ctcttcaccc agcgcgtgaa gtgcgatccc cgccgtaagc
     4021 aaaaggtccg aagccgctag ggccagccag aactggctac gtcccaccga gttgcgtgcg
     4081 gcggcggcca gcgccgcgct cgaggtccac cagaagagca gagccggcac cgcccacgcg
     4141 ttcaacgcga acaggaccag accacacgag ccgacaccgg ccaggcacgc gggctcccac
     4201 cggccccgac ctttgtcgag gctgagcacg agcagccacg tgcacgcaca ggcgacgcct
     4261 gctgcaaccg tcggacccgg atccgggtcg agcgttctcc atttcagccc tgaatgtctg
     4321 aggaagaaca ggatcgcacc cagagtcacc ccgacaaggc tgatgacctg cacgccgcgc
     4381 gctctgactt cctctgcgtc gcgcataacg aatgcgatca gcgcgagcac g
//
LOCUS       DATMED010000391         4908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5682254, whole genome shotgun sequence.
ACCESSION   DATMED010000391 DATMED010000000
VERSION     DATMED010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5682254"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VNP73_10185"
     CDS             <1..817
                     /locus_tag="VNP73_10185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWL66329.1"
                     /translation="TGHLLLTERPDDRDELVDIAERQSDAGIETTVVEGTELKALEPG
                     LAPEVLAAAWCPGDGVAAHTATTQIVAAAATEAGVEFRLKNPVSSLDAIDAVDGILIA
                     ANQGTAELLRLIDRALPLFPVWPQVVMAEAEGRSPQTLIGHVHRRLVVKRMDAHTVMI
                     TGGRLGAVQPDGSVGVEPSEIAATLGDASSVFPELVDATVTVAVADRSESVTPDLVPI
                     VDRIEHDPPVWIATGWSGHGFALAPAVARSIASWMSDGERPGPLAPFSVERFD"
     gene            complement(814..3810)
                     /locus_tag="VNP73_10190"
     CDS             complement(814..3810)
                     /locus_tag="VNP73_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HWL66330.1"
                     /translation="MHIKHFRPGALALALATALAIVPLPALAQVDPSTTDAPILSREA
                     PRGTGTTEPSGHAAPVGKVVDGNISDWISRSSLFGGTVVYSGGELVYQDHLFDAYGPD
                     DGRDADRLAKTDGVEEVLPEAYRIDALAQANAPDQVGAPVPPGTEQFMYDETYGDSTS
                     DQDHADLTQLRLAREGDSLALLARTTTMRADSKTALLVLLDSVEGGLGDVGFGSGVTS
                     AEADAAVFLTRDSGAVSVGGGPPVALPAGSVASDPSGYTNSIEARIPLSAFGGPNALR
                     VAAATGTANAAGDGFAPLEIEQNNDVEHANLANVAFRLDEPVRIWFEKEQALSLHSGS
                     IDRFFHEVDEQKLGSGVVERYVPGTGYHDRIFISSEQVASEQGENGIFQHYGVFLPTA
                     YDGATPLPLQWWLHWRGGSAHSGGAIVPRVFKHFGEDVNSIVVAPSGRGSSYWYVGKG
                     HVDFREVWADVFDTFAVDRNRVYSSGHSMGGFGTYLLTLLYPDRFAAGAPVAGPVTQG
                     AWTGLDFPGCDQFDADGNTPCFIEANGSEPRAQHTRKLLGNARHVPYAILHGTDDELV
                     FYSGVARQAEQLTLLNYRHRFYTYPGYEHFTHPVMDQWAEASRYMHTVATPEHPRQVT
                     YTRDMGFERATERVQAANLGLDFSFDSAYWMSGLAPAGEPAAANETETRAAVFDGVSL
                     AVSEPPHLVLPDAGGPASPGTTGPYFYEGLQWLADPSATAPEAINAFDITLTGAKAVS
                     LDLAGMQIDPTRVISGAVTTDPGLDLSLKGDWTRAPDVTIDGQPVSAALNGDMLLVNV
                     PAGDHVLEITPGTQSPNEPVIAFTSRSATSGQYSDEATLEARLVDASGAALSERTVEF
                     VLTGPAGSQAFNALTNADGIATLSLSLNDVPGDYWVTARHALSEGSIEASSPFTIERE
                     DTALSLSTQGNGAKKTLRGVLVDPDAGGIAGRAITFTSNGETLGTAITDENGVATLAV
                     PAGARGGNRSYSASFDGDEFYKPSSG"
     gene            complement(3859..>4908)
                     /locus_tag="VNP73_10195"
     CDS             complement(3859..>4908)
                     /locus_tag="VNP73_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWL66331.1"
                     /translation="RELEAQLAAIVASTDDAIYSKDSEAFITTWNHAAESLYGYTAEE
                     AIGQPVRMLLPDERRGEEREILDKIMVGEQVDHYETRRITKDGRLIDVEITASPMHDA
                     NDQIVGASVIARDIADRKKADAMAQQLERQEFVNLVAHELRSPLAAITGASDLLSGTA
                     ETLSEQGQKLVDMIARQTRVAQRLINDLLDLSRIQSARFEVNLTELSLAAEVHDVLEL
                     LPAPEGKTVRVDVPEGLSVVADQDRLRQVMTNLIANAFKYGGGEVTLAGRKLDASVEL
                     IVADNGEGLSDEVADRAFEPFVRGRSVSRERGSGLGLSITKALMEAFDGRIRYERSPT
                     GAAFVCTFHGSFQDR"
BASE COUNT          856 a         1520 c         1628 g          904 t
ORIGIN      
        1 gacgggtcac ctgttgctga ctgagcgccc cgacgatcgc gacgagctgg tcgacatagc
       61 ggaacggcag agcgacgcgg gcatcgaaac cacggtcgtg gaggggaccg agttgaaggc
      121 tctcgaaccc gggctcgcgc cggaagtgct cgctgccgcg tggtgtcccg gcgacggcgt
      181 tgccgctcac accgcgacga cgcagatcgt cgcggctgct gcaaccgaag ccggagtgga
      241 gtttcggttg aagaaccccg tctcgtctct cgacgcgatc gatgctgtcg atgggatcct
      301 catcgcggcg aatcagggga cggccgagct gctccgcctg atcgaccggg cgctgcccct
      361 attcccggtc tggccccagg tcgtgatggc ggaagctgag ggtcgatcgc cccagacatt
      421 gatcggtcac gttcaccgca ggctcgttgt caagcgtatg gacgcgcaca cggtgatgat
      481 cacgggggga aggctgggcg cggtacagcc ggacggaagc gtcggcgtcg agccgagcga
      541 gatcgcggca acgctcggcg atgcatcgtc cgtgtttcca gaactggtcg acgcgaccgt
      601 gacggtcgcg gtggccgacc gctccgaatc agtgaccccg gatctcgttc cgatcgtgga
      661 ccggatcgaa catgaccctc ccgtgtggat cgctacggga tggagtgggc acggattcgc
      721 gttggctccg gccgtcgctc gatcgatcgc gtcgtggatg tcggatgggg agcgtcccgg
      781 ccccctcgcg ccgttctcgg ttgagaggtt cgactagccg gacgacggct tgtagaactc
      841 gtcgccgtcg aaggacgcgg aatagctgcg gttcccgccg cgggctcccg ccggtacggc
      901 cagggtcgca acgccgttct cgtcggtgat cgcggtgccc agggtctctc cgttggacgt
      961 gaacgtgatc gcgcggccgg caatgccccc ggcatccgga tctacgagca cgcctctcaa
     1021 ggtcttcttc gcgccgttgc cttgggtcga cagcgacaga gcggtgtctt cgcgttcgat
     1081 cgtgaaggga ctgctcgcct cgatcgaacc ctcggatagc gcgtgcctcg ccgtgaccca
     1141 gtaatcgcct gggacatcgt tcagggacag cgagagagtc gcgatcccgt cggcgttcgt
     1201 aagagcgttg aacgcctgcg atccggccgg gccggtgagg acgaactcga ctgtgcgctc
     1261 ggagagggct gcgcccgacg cgtcgaccag ccttgcttcg agcgtggctt cgtcggagta
     1321 ctgaccggac gtggcggaac gcgatgtgaa tgcgataacc ggttcgttcg gagactgcgt
     1381 tccgggggtg atctctagga cgtgatcgcc ggcgggaacg ttcaccagca gcatgtcccc
     1441 gttcagtgcc gccgaaaccg gctgaccgtc gatggttacg tcgggtgcgc gcgtccaatc
     1501 gcctttcagt gacaggtcca agcccggatc ggtcgtaacc gctcccgaga tgacccgtgt
     1561 gggatcgatc tgcatgccgg cgaggtccag cgacacggct ttcgcccccg tcagggttat
     1621 gtcgaacgcg ttgatggcct ccggtgcggt agcggatggg tccgccaacc actgcaatcc
     1681 ttcgtagaag tagggaccgg ttgtgcccgg ggatgcaggc ccaccggcgt cggggagcac
     1741 aaggtgcggt ggctccgaca cggccagact gacgccgtcg aaaaccgcgg cgcgggtctc
     1801 ggtttcgttc gcagctgcag gttcaccggc cggagcgagg ccggacatcc agtaagcgga
     1861 gtcgaacgag aagtccagcc cgaggttcgc ggcttgaacg cgttcggttg cccgctcgaa
     1921 acccatgtcc ctcgtatagg tgacctgacg cggatgctcg ggggtcgcaa cagtgtgcat
     1981 gtagcgcgag gcttccgccc attggtccat gaccgggtgg gtgaagtgtt cgtagcccgg
     2041 ataggtgtag aagcgatggc gatagttcag aagcgtcagt tgctcggcct ggcgagcgac
     2101 gcctgaatag aagacgagct cgtcgtccgt cccgtgcaag atcgcgtaag gaacgtgcct
     2161 cgcgttaccc agcagctttc tggtgtgctg cgccctcggc tcgctaccgt tcgcctcgat
     2221 gaagcagggc gtgttgccgt ccgcatcgaa ctggtcgcac ccggggaagt cgaggccggt
     2281 ccaggcccct tgtgtgaccg gtcccgccac cggagcgccc gccgcgaaac gatccggata
     2341 aaggagcgtc agtaggtacg ttccgaaacc acccatcgag tgaccggacg agtagacacg
     2401 attgcgatcg acggcgaacg tgtcgaacac gtctgcccat acctcgcgga agtcgacgtg
     2461 ccctttcccg acgtaccagt agctgcttcc gcggcccgaa ggcgctacga cgatcgagtt
     2521 cacgtcttcg ccgaagtgct tgaagacgcg ggggacgatc gctccacccg agtgggcgct
     2581 gcctcctcgc cagtggagcc accactgcag aggcagaggc gttgccccgt cgtaggcagt
     2641 cggaaggaag acgccgtagt gctggaagat gccgttttcg ccttgctcgg acgcgacctg
     2701 ttccgaagag atgaagatgc ggtcgtggta gccggtgccg ggtacgtaac gctcgacgac
     2761 tccggaaccg agcttctgtt cgtctacctc gtggaagaaa cggtcgatcg agcctgagtg
     2821 caagctcagt gcttgttcct tctcgaacca gatgcggacg ggctcgtcca acctgaacgc
     2881 aacgttcgcc agattcgcgt gttcgacatc gttgttctgc tcgatctcca gcggagcgaa
     2941 gccatcgccg gccgcgttcg cggtcccggt cgcggccgcc acccggaggg cattcggtcc
     3001 accgaaagca cttagaggga tcctcgcctc gatcgagttc gtgtaccccg aaggatcgga
     3061 tgcgaccgag cccgcgggaa gggcgaccgg cggaccccca ccgaccgaca cggcaccgga
     3121 gtcgcgtgtg aggaatacgg ctgcgtccgc ctctgcgctg gtgacaccgc ttccgaagcc
     3181 gacatctccg agaccaccct cgacggagtc gagaaggacc aggagagccg tctttgaatc
     3241 ggcgcgcatc gtcgtggttc gggcgagaag cgcgagcgag tcgccctccc gcgccaaccg
     3301 cagttgggtg aggtcggcat gatcctgatc ggaagtggaa tccccgtagg tctcgtcgta
     3361 catgaactgc tcggtgcccg gcggcacggg cgcacccacc tgatcagggg cattggcctg
     3421 ggccagtgca tctatccggt acgcctcggg cagcacctct tcgacgccat cggttttggc
     3481 cagcctgtcg gcgtccctgc cgtcgtcggg gccgtaggca tcgaatagat gatcttgata
     3541 gacaagctct ccgcccgagt acacaaccgt tcctccgaac aaactggacc ggctgatcca
     3601 gtcggagatg ttgccgtcta cgaccttgcc caccggagcg gcatggccgg aaggttcggt
     3661 cgtaccggtt ccccgaggag cctcccggga caggatgggg gcatcggtgg tggacgggtc
     3721 tacctgagcg agggcgggca gaggaacgat ggcgagggcg gttgccaggg cgagcgcgag
     3781 cgcgccgggc cgaaagtgct tgatatgcat gctggaggat tcgctgcgga gaagcgggtt
     3841 ccttcgaggt ggaccgaact agcgatcttg gaaagaaccg tggaacgtgc agacgaaggc
     3901 cgctccagtg ggtgatctct catagcggat ccgtccgtcg aaggcctcca tcaaagcctt
     3961 cgtgatcgac aatcccaggc ccgagccgcg ttcgcgcgag acactgcggc ccctcacgaa
     4021 aggctcgaac gcccggtcgg cgacctcatc ggacagcccc tccccgttgt cggcgacgat
     4081 gagctccacc gaagcgtcca gcttccggcc ggcgagggtc acctcgccgc ctccgtactt
     4141 gaacgcgttg gcgatcagat ttgtcatcac ctgtctcaac cgatcctgat ccgcaacgac
     4201 acttagaccc tcgggaacat cgacccggac ggtcttgccc tcaggggccg gaagcaactc
     4261 cagcacgtcg tgaacctcgg cggccaagct gagctcggtg aggttgactt cgaaccgggc
     4321 actctggatc cgggaaagat cgagcagatc gttgatgagt ctctgggcaa cccgggtctg
     4381 gcgggcgatc atgtcgacca acttctgtcc ttgctcgctg agggtctccg cggtcccgga
     4441 caggaggtcg gaggctcccg tgatcgccgc gagaggcgag cgcaactcgt gagctaccag
     4501 gttgacgaat tcctgacgct cgagctgctg agccatggca tccgctttct tccggtcggc
     4561 gatgtcacgc gcgataaccg acgcaccgac gatctggtca ttcgcgtcgt gcatcggaga
     4621 ggcggtgatc tccacgtcga tgaggcgacc gtccttggtg atgcgtctcg tttcgtagtg
     4681 atcaacctgc tcgcccacca tgatcttgtc gaggatctcg cgttcctcgc cccttctctc
     4741 atcgggaagg agcatccgaa ccggttgacc gatggcttcc tcggcggtgt agccgtacag
     4801 gctttcagcc gcatggttcc aggttgtgat gaaggcctca gagtcttttg aatagatcgc
     4861 gtcatcggtg gacgcaacta tcgctgcgag ctgagcttcg agttctcg
//
LOCUS       DATMED010000392         2848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2252626, whole genome shotgun sequence.
ACCESSION   DATMED010000392 DATMED010000000
VERSION     DATMED010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252626"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..818
                     /gene="recO"
                     /locus_tag="VNP73_10200"
     CDS             96..818
                     /gene="recO"
                     /locus_tag="VNP73_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWL66332.1"
                     /translation="MPGLYKDEGIVLKTIKLGEADRIVTLLTRDTGKVRAVAKGIRKT
                     KSRFGARLEPFTHAHLMIYKGRNLDTITGVDIVDSFDAVRRDYLGLTSAAALVEVVEK
                     ITLDREPSLSVYTLLLDGLQALASAPGPTIVPAFLVKLLSVSGYHPQLTVCAGCGDGT
                     VLAGFSPSLGGAVCESCWREDRGALALAADRIDLLNRLLDSEFGHAADAGAVVEVTRA
                     LRAYTEYYLERPLKSLQLLAAG"
     gene            821..1489
                     /gene="uppS"
                     /locus_tag="VNP73_10205"
     CDS             821..1489
                     /gene="uppS"
                     /locus_tag="VNP73_10205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWL66333.1"
                     /translation="MANAAPAGLPAHIAIIMDGNGRWAAARELPRTEGHTAGERALWD
                     VIEGALDIGIRYLSVFAFSTENWMRPREEVDFLFNFNRSLLKQRRNSLKEMGVRIRRI
                     GRREPVPQDVLAEFDEAEEMTAANDRMHLLVCFNYGGRAEIEDAAKKGPIADNLYASD
                     VPDVDLLIRTSGEKRLSNFLLWQAAYAELYFTDILWPDFDRASLAEAVEDYAERERRF
                     GGLT"
     gene            1486..2727
                     /locus_tag="VNP73_10210"
     CDS             1486..2727
                     /locus_tag="VNP73_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HWL66334.1"
                     /translation="MSEDLLEIADSIWSGEVSMEEKHPMAIPGQLLEVRPGVAFVSGF
                     SNAIAFDTGDGLVLIDSGGFLQPDYVHGHVRGWSDQPLRFAIYTHGHVDHVFGVGPFD
                     SEPGEPVVIGHENVPPRFDRYVLTAGYNSVINQRQFQVPGLQWPTEYRFPDITYRDDY
                     VLEHGDLRFELYHAKGETDDHTWVWVPDRRVLCCGDLFIWCSPNAGNPQKVQRYPREW
                     AEALRAMQDVGAEVLLPGHGLPVVGTARIQEALDSTASYLESICDQSLALMNEGLSLD
                     EIVARVEPPAELAAKPFLQPIYDEPDFIVRNLWRLYAGWYDGDASRLKPSAPGELDSA
                     LVGLVGGVEPLVAEARRRADAGELALACELIEIAHAAAPDDADVKTARSDIYARRSKV
                     ERSTMAKGVYAWAAAESSASG"
BASE COUNT          495 a          881 c          925 g          547 t
ORIGIN      
        1 tttccgcaag gtcgagtcct ttctcttgga cggatggtcc cgcccgatcc ttcgccatcc
       61 acgtctcgga ctcctccctc tctcataatg aggacatgcc cgggctctac aaagacgagg
      121 ggatcgtcct caagacgatc aaactcggag aagcggaccg catcgtcacg ttgctgacgc
      181 gcgacaccgg caaggtccga gcggtcgcga aggggatccg caagaccaag agccgcttcg
      241 gcgcccggct cgagccgttc acccatgccc acctgatgat ctacaagggg cgcaacctcg
      301 acaccatcac gggggtagac atcgtcgact cgttcgacgc ggtccggcgg gactatctcg
      361 ggctgacctc cgccgcggcc ctggtggagg tcgtcgagaa gatcacgctc gatcgcgagc
      421 cttcgctctc cgtgtacacg ttgttgctcg acggacttca agcattggcc tcggcccccg
      481 gtccgacgat cgttccggcc ttcctcgtca agctcttgtc ggtgtcgggc taccacccgc
      541 aactcacggt ctgcgccgga tgtggcgacg gcacggtgct ggcggggttc agcccgtcgc
      601 tgggtggagc ggtatgtgag tcgtgctggc gtgaagatcg aggtgctctc gcgctcgctg
      661 ccgatcggat cgacctgttg aacagattgc tggactcgga gttcggccac gcagccgatg
      721 cgggggccgt ggtggaagtc acgcgcgcat tgcgcgcgta caccgagtac tacctggagc
      781 gaccgctcaa gagcctgcag cttctcgcag ccggctgagc atggcgaacg cggcgcccgc
      841 cgggttaccg gctcacattg cgatcatcat ggacggcaac gggaggtggg ctgcagcacg
      901 cgagcttcct cgcaccgaag ggcacaccgc cggcgagcgc gctttatggg atgtgatcga
      961 gggtgcgctc gatatcggta tcagatatct atccgtattc gcgttctcga cggagaactg
     1021 gatgcgaccc cgcgaggagg tcgacttcct cttcaacttc aatcgctcgt tgctgaagca
     1081 gcgcaggaac tcacttaagg agatgggtgt tcgcatccgc cggatcggtc gccgcgaacc
     1141 ggtgccccaa gacgttctgg ccgagttcga cgaagcggaa gagatgacgg ccgccaacga
     1201 ccgcatgcat ctgctggtct gtttcaacta cgggggccga gccgagatcg aggacgcggc
     1261 caagaaaggg ccgatcgcgg acaatctgta cgcctccgac gtacccgacg tggatctcct
     1321 gatccgtacc tcgggcgaga agcgcctgtc caacttcctg ctgtggcagg ccgcttatgc
     1381 cgagttgtac ttcaccgaca tcctgtggcc ggacttcgac cgtgcttctt tggccgaagc
     1441 agtagaggat tacgccgaac gagaacgtcg attcggagga ctgacgtgag tgaagacctg
     1501 ctggagatag ccgattccat ctggagcggc gaggtgtcga tggaagagaa acatccgatg
     1561 gcgatcccgg ggcagctgct cgaggtccgt cccggcgtcg cgttcgtctc gggtttctcg
     1621 aatgcgatcg cgttcgatac cggcgacggt ctcgttctga ttgacagcgg cggtttcttg
     1681 cagccggatt acgtgcacgg ccatgtgcga ggttggagcg accagccgct ccgtttcgcg
     1741 atctacacgc acggacacgt cgaccatgtc ttcggggtcg gcccgttcga ttccgagcct
     1801 ggcgagcccg tcgtgatcgg gcacgagaac gttccgccgc gctttgatcg ctacgttctg
     1861 actgccggtt acaactccgt gatcaaccag aggcagttcc aggtcccggg gttgcagtgg
     1921 ccgaccgagt accgcttccc ggacatcacc taccgggacg actacgtcct ggaacacggc
     1981 gacctcaggt tcgagctgta tcacgcgaag ggggagaccg acgatcacac ctgggtctgg
     2041 gttcccgatc gacgggtgct ctgttgcggg gatctgttca tctggtgctc gccgaacgcg
     2101 ggcaatccac agaaggtgca gcgctaccca cgcgaatggg ccgaagctct gagagcgatg
     2161 caggacgtgg gggccgaagt cttgttgcct ggacatgggt taccggtggt cggaactgca
     2221 cgcatccagg aagcactcga ttcgactgcc tcttatctgg agtcgatctg cgatcagtcc
     2281 ctcgcgttga tgaacgaggg actgtccctg gacgagatcg tcgctcgggt cgagcccccg
     2341 gcggagctgg ccgcgaagcc tttcctccag ccgatctacg acgagcccga cttcatcgtg
     2401 cgcaacctgt ggcgtctata tgcgggttgg tatgacgggg atgcatcacg attgaagccc
     2461 tcggcccccg gcgagctgga ctccgctctg gtcggactcg tcggtggtgt cgaaccgttg
     2521 gtagccgagg cccggcggcg cgccgacgcc ggcgagctcg cgctggcgtg cgagttgatc
     2581 gagatagctc acgccgcggc ccccgacgat gccgatgtaa agaccgctcg ttcggacatc
     2641 tacgcccggc gttcgaaggt cgagcgctcg acgatggcga aaggcgtcta cgcctgggcc
     2701 gcggctgagt cgtccgcctc gggctaaggc gggttgtctt cgtcgatgtg ttgctcggcc
     2761 gacttggcgc cgggaccgac ggccgccgcc agcacccccg cgttgggcaa caacaccggg
     2821 ccgtgatcgg tgttcatgtg cacgtaga
//
LOCUS       DATMED010000393        10995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1461719, whole genome shotgun sequence.
ACCESSION   DATMED010000393 DATMED010000000
VERSION     DATMED010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461719"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VNP73_10215"
     CDS             complement(<1..558)
                     /locus_tag="VNP73_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWL66335.1"
                     /translation="MKTTEQDVLWPEEIPSLSSGALETQEMVLNVGPQHPATHGVFRL
                     LATIDGEVISDAEPVIGYMHRGYEKLVEARDYRQITQLVSRMDWLSGINNELPMWLAV
                     EELMEVEVPPRAQYMRVILCEMNRILNHVMFFQSFAAELGSLTPTFYAFREREDIQYA
                     MEMATGGRMHFGFMRCGGIKEDVPRG"
     gene            complement(558..1115)
                     /locus_tag="VNP73_10220"
     CDS             complement(558..1115)
                     /locus_tag="VNP73_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWL66336.1"
                     /translation="MPILQRVTGEDAIARVGAAVGDLATDVKDSHGHVDLVCEAGKLP
                     DVMQRLRDADGLRCRFFTFLSGIDRTELEEDKTSYGTGLEVLVHVYSPEFGTHVNVHV
                     PLPAENATCPTITGIYMGAFWHERECHEMFGIDFEGHPKLVNLYLTEDFEGHPGLKSF
                     KLPARTTVKDWPGAKDPEEAAAGGH"
     gene            complement(1120..1644)
                     /locus_tag="VNP73_10225"
     CDS             complement(1120..1644)
                     /locus_tag="VNP73_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWL66337.1"
                     /translation="MLEKVKLPKPVSSLLNWSRKYSLWCMQFGLACCAIEMGAAFASK
                     FDVIRFGVIPFPASPRQSDLLVVAGTLTDKMAAPLKRVYDQMPEPKYVISMGSCANCG
                     GPYWDSYSVTKGCDQVIPVDIYVPGCPPRPEALLQGVIRLQEKITNEEIATKWKGHDT
                     PIPHIPEAGLAGGI"
     gene            complement(1655..2023)
                     /gene="ndhC"
                     /locus_tag="VNP73_10230"
     CDS             complement(1655..2023)
                     /gene="ndhC"
                     /locus_tag="VNP73_10230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWL66338.1"
                     /translation="MPEGYFGQYATIGIFAIAGVLIVVGTIGVSRLIQPRVPHPDKLT
                     TYESGIDPIGQGWSQANVRYYIFALLFVVFDVEAVFLFPWAVVFERLGTQAFVEMLIF
                     IGILAIALIYAIKKKVLEWL"
     gene            complement(2193..3683)
                     /locus_tag="VNP73_10235"
     CDS             complement(2193..3683)
                     /locus_tag="VNP73_10235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWL66339.1"
                     /translation="MPFDPPAVELAGIAPEIALTITALFLMVFDAFVVRIAPSHSRWY
                     SPVIATLGLTVATLLAVSSWGANEVQLGGAIALDGFAIFVKIGLAVFATLTVWLARDY
                     LAREGLEDAEFYVLILFATAGMMLMAAAADLIVVFLALETFSLAIYVLVGFRRRSTES
                     QESALKYFLLGAFSSAFFLLGVALAYGAAGTTAFYSGGGPSESISRYLGSTPTADLAL
                     MVVATGFLIVGMGFKVAAVPFHAWTPDAYQGAPAPVTGFMAAATKIAAFAATLRLLDA
                     VLFPLRWDWQPLILVLATVTMVVGSVVAIVQEDLKRLLAYSSIAHAGFILTGVVAAND
                     DGISGSLFYLVTYGVTVLGAFAVLSVLSGKDEQRIRMIDFKGLFYESPLLAGAFTLFL
                     LSLAGVPITSGFVGKLVVFGAAVEAGYAWLAVVGVLASAIAAFFYLRVLVVMYMQEPD
                     EPRDPVRPGVLAAGVVSVAAASTVLFGLLWGPLVEAAQQATFIAGR"
     gene            complement(3684..5189)
                     /locus_tag="VNP73_10240"
     CDS             complement(3684..5189)
                     /locus_tag="VNP73_10240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWL66340.1"
                     /translation="MSRFSMLDLTIFLPLVGALLLGFVPRDNLRAVRLTALAVTLVAF
                     VTSLFVLAQFEAGVAGFQLVTSVQWIPEWGVGYTTAVDGVSLWMILLTTFLMPLGVLA
                     SWSITKRAKPYFIFLLALETGMLGVFAALDLFLFYLFWEATLVPMYFLIGMWGYSKRI
                     YAAMKFFLFTLVGSLLMLVAILALYFANEGLRSFDYRVLLGTPLSLEVQRLLFLAFFA
                     SFAVKVPLVPFHTWLPDAHTEAPTAGSVMLAGVLLKMGAYGLIRFGIPLFPDAADQLA
                     PLVVALAMIGIVYGALVAMMQKDLKRLIAYSSVAHLGFVVLGVFVGNTEAMSGSILQM
                     VNHGLSTGALFMLVGALYDRRHTRAIADFGGLAKSIPIFSGVFLVVALSSLGLPGLNG
                     FVGEFLILLGTFFSYGWWVIPAAFGVVLAAIYMLRSYQRVFHGEPTSKENRELPDLSP
                     RELAMVVPCVALIVFIGIYPKPFLDRIEPSAERHLVTRPAGSDSVTAEGGR"
     gene            complement(5197..7143)
                     /gene="nuoL"
                     /locus_tag="VNP73_10245"
     CDS             complement(5197..7143)
                     /gene="nuoL"
                     /locus_tag="VNP73_10245"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWL66341.1"
                     /translation="MELIPGSDVLVLSLSTFTFPLATAIVLLLFGTRLEAMSIFGMRV
                     KGWTGWLASIALARSFLSAVVLFFNLLGMPAEEREVVVTAFEWIKAGSFSAAFDLRID
                     PLSAVMLLVVTGVGTLIHIYSIGYMHGDSRFSRYFAYLNLFAASMLILVLADNLLLLY
                     VGWEGVGLCSYLLIGFWFEKPSAHNAAKKAFLVNRIGDFSFLLGMLLLAGTVGSLSAD
                     AVNASAEGGAMSAGVATVAALLLFGGATGKSAQIPLYVWLPDAMEGPTPVSALIHAAT
                     MVTAGVYMVARFSPVFEHAGPEALMVVGWIGALTALMAALMASVQYDIKRVLAYSTIS
                     QLGYMFLAHGVGAYSVGIFHLVTHAFFKALMFLGAGAVMHALGGETDIRKMGGLRHHL
                     PLTSSSFALGWLAICGLIPFSGFFSKDAILASAWSEGQYALWGIGVATAFLTAFYMSR
                     LYFRVFAGRPRYADDKHIHDAPPSMAFALVVLGVLSASGGMINLPGVLSLEVFLEPVV
                     GEAHVPTGLTPWVLGGIALLVAVAGIFFARSLYLTRAGSLRRRRIVSRVRPLVTAAQN
                     KFWLDEIYGATIVGPGKRFAAYLSDVFDRRYVDGAITGLGRFVANAAESLRGLQTGYV
                     RNYAATFLFGVVLLISILVVRVAV"
     gene            complement(7143..7421)
                     /gene="nuoK"
                     /locus_tag="VNP73_10250"
     CDS             complement(7143..7421)
                     /gene="nuoK"
                     /locus_tag="VNP73_10250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007320414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWL66342.1"
                     /translation="MLAALMFTVGVVGALVRRNAIVIFMFIELMLNAVNLFFVTFSRM
                     HGHIDGQVLVFFVLVVAAAEVVVGLAIITLIFRHRTTANVDDAHLLRR"
     gene            complement(7445..7999)
                     /locus_tag="VNP73_10255"
     CDS             complement(7445..7999)
                     /locus_tag="VNP73_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWL66343.1"
                     /translation="MNLEQILFGVMFIVALGSSLAMLFARNAVHTALFLVAAQIALAV
                     AFLLQGAFFIAAIQIIVYAGAIMVLFLFVIMLLGVDRREALIEPLPGQRSLAIGLGAL
                     LAAQIAYVAWRGVGAISEARRGAEALTALNRDPGNVESIARALFSRYLLPFEATSVLL
                     VVAIVGVMVLARRLSAVGQDGAAE"
     gene            complement(7996..8505)
                     /gene="nuoI"
                     /locus_tag="VNP73_10260"
     CDS             complement(7996..8505)
                     /gene="nuoI"
                     /locus_tag="VNP73_10260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWL66344.1"
                     /translation="MALPEIFRGMKVVIANVFKRKETIAYPEVRRRPFPRFKGRHILD
                     RHEDGLEKCIGCELCAGACPADAIYVVGAENTPEQRFSPGERYAQIYQINFLRCIFCG
                     LCVEACPTEALLMSTEYEISGSSREELIFTKEQLIVDAPIVRHWPTKKELEPEHNFDV
                     IKSGRKDSS"
     gene            complement(8492..9712)
                     /gene="nuoH"
                     /locus_tag="VNP73_10265"
     CDS             complement(8492..9712)
                     /gene="nuoH"
                     /locus_tag="VNP73_10265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWL66345.1"
                     /translation="MIEFIGVNVIIAAVKVIVIFLLLLVVTILVIWFERKVIADMQGR
                     IGPNRWGPFGLLQTLADGIKLFFKEDFRPASADKWTYAIAPVAAMVPAFLAFAVVPIG
                     DRITVGGEKISLIVSDLNIGILYFLAMGSLGVYGVVLAGWASGSKYPLLGAIRSSAQM
                     ISYEIALGLSLVPIALVAGSLSTVDIVAAQAGTVGNVTFFDGIPVLEQVSQIFKYAPD
                     WYIWSQWPAFLIFMVAGIAETNRAPFDLPEAETELVAGFHTEYSGIRFAMFFLGEYIH
                     VVAVSAMAVTLFFGGWHGPSFGFLPWVWPLLWFTLKTVAFIYLFVWLRATLPRLRYDR
                     LMWLGWKRLIPASLGWILVTAVVNTDGIGRGVRIGTFAALFMIVMVWISRGDPRLKEA
                     VTPKPAKEAVHGAA"
     gene            complement(9709..>10995)
                     /locus_tag="VNP73_10270"
     CDS             complement(9709..>10995)
                     /locus_tag="VNP73_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWL66346.1"
                     /translation="ADLDALGDIEGPVVVAWGPSSPGRDESAWASAALNLAQRTDAKV
                     LVCPPHAGSQGLLDMGAHPALGSTDETGRDTRAILEAAVAGEIDVLLIFGADPIRDFP
                     DAELARRALLSVKFALVVELFPTETVQHADVVLPSAAYAEREGTFTNLERRLQKLEPL
                     LAPPGTAREPWRVAAGIARGLDADWGWQGCDDVWDDIRSNVPTHAQVETAALTQEMPV
                     HSLQYETGFASKSHEAQTSVAGPGGQYPKGYRSGAPFQTGNAWPLSWELRAFEARQRP
                     GYVPEIPDQHQPEDGDPAAAQESRAAAGDLRPTAPNRSESPSDPSSSSPSGSTFDLYS
                     GRFIYDEGSMVSKSRALHGLQKKPFVEMNDEDAKALGVADGDMVRVIAGDVEVELPVR
                     IDDIVRGAVFVPYDQAGLCANTLMRGIDPTVEVRPA"
BASE COUNT         2430 a         3466 c         3373 g         1726 t
ORIGIN      
        1 acccctaggc acgtcttcct tgatgccgcc acagcgcatg aagccgaagt gcatccggcc
       61 cccggtggcc atctccatcg cgtactggat gtcctcgcgt tcccggaatg catagaacgt
      121 cggtgtaaga gagccgagct cggccgcgaa cgactggaag aacatgacgt gattcaggat
      181 ccggttcatc tcgcacagga tcacgcgcat gtattgcgct ctcggcggga cctcgacctc
      241 catcagctcc tcgaccgcca gccacatcgg cagctcgttg ttgatccccg atagccagtc
      301 catgcgcgac acgagctgag tgatctgtcg gtaatcacga gcttcgacga gcttctcgta
      361 gccgcggtgc atgtagccga tcacaggctc ggcatcgctg atcacttctc cgtcgatggt
      421 cgccagaaga cggaacaccc cgtgggtcgc cggatgctgt ggcccgacgt tgaggaccat
      481 ctcctgcgtc tcgagcgcgc cgctcgaaag cgacgggatc tcttcaggcc acagaacgtc
      541 ttgttcggtc gtcttcatca gtggcccccc gccgcagcct cttcgggatc cttagcgccg
      601 ggccagtcct tgaccgtcgt tcgggccggc agcttgaagg atttgaggcc ggggtgacct
      661 tcgaagtcct cggtcaggta caggttcacg agtttggggt gaccctcgaa gtcgatcccg
      721 aacatctcgt ggcactcacg ctcgtgccag aacgcaccca tgtagatgcc ggtgatcgtc
      781 gggcacgtgg cgttctcggc aggcagagga acgtggacat ttacgtgggt tccgaattcg
      841 ggtgagtaga cgtgcaccag gacttccaaa cccgtgccgt aggaggtctt gtcctcctcg
      901 agctcggtgc gatcgatgcc ggagaggaag gtgaagaagc gacaccgcag cccgtccgcg
      961 tcgcggagcc tctgcatcac gtcggggagc ttgccggcct cacaaacgag atcgacatga
     1021 ccgtggctgt ccttcacgtc cgtagcgaga tcgccgacgg cggctccgac gcgcgcgatc
     1081 gcatcctcgc ccgttactcg ctgcaagatc ggcatgcggc tagatgcccc cggccagtcc
     1141 ggcctctggg atgtgaggga tcggggtgtc gtgacccttc cacttggtgg cgatctcttc
     1201 gttcgtgatc ttctcctgca gacggatgac gccttgcaac aacgcctcgg ggcgcggagg
     1261 gcagcccgga acgtagatat cgacggggat gacctggtca caacccttcg tcaccgagta
     1321 cgagtcccag tagggcccgc cgcagttcgc gcacgacccc atcgagatca cgtatttcgg
     1381 ctcgggcatc tggtcgtaaa cacgtttgag cggcgccgcc atcttgtcgg tgagggttcc
     1441 cgcgacgacc aatagatccg actgacgcgg cgaagccggg aacgggatga ccccgaatcg
     1501 gatcacgtcg aacttgctgg cgaaggcggc ccccatctcg atggcgcaac aggcaagccc
     1561 gaactgcatg caccacagcg agtacttgcg ggaccagttg agcagcgaag agacgggctt
     1621 cggaagcttg accttctcta acatctttcc tccgtcacag ccattcgagg accttcttct
     1681 tgatcgcgta gatgagggcg atggccagga tgccgatgaa gatcagcatc tcgacgaagg
     1741 cttgggtgcc cagccgctcg aagacgactg cccaggggaa caggaacacg gcctcgacat
     1801 cgaagaccac gaacagcagg gcgaagatgt agtagcggac gttcgcctgg ctccaccctt
     1861 ggccgatcgg gtcgataccc gattcgtagg tcgtgagctt gtcgggatgg ggaacccgag
     1921 gctggatgag gcgggagaca ccgatcgtcc cgacgacgat caggaccccg gcaatcgcaa
     1981 agatgccgat cgtggcgtac tggccgaagt aaccctcagg cacttggtcg tctcgctccc
     2041 gtcgcggtcg gctcggtcga tctcatcagt ggggtccaag gaagtaaaag ccctggatac
     2101 caggcgaaaa ctaacacctg agtcccggcg cgaccctcat tcgtaccctc gtgaagttcc
     2161 tggtagatca cttgcgcgac ggccggttcc cgtcaccgac cggcgatgaa cgtggcctgc
     2221 tgcgcggcct cgacgagggg cccccacagc aagccgaaca aaaccgtcga agccgccgct
     2281 acggacacga ccccggctgc aaggacgccg ggccggaccg gatccctcgg ttcgtccggc
     2341 tcctgcatgt acatgacgac gaggacgcgt agatagaaga acgcggcgat cgcgctcgcc
     2401 agcacgccga cgaccgccaa ccacgcgtaa ccggcttcta ccgcggcgcc gaacacgacg
     2461 agtttcccga cgaaccccga ggtgatcggg acccccgcga gtgacagaag gaacaaggtg
     2521 aatgctcccg ccagcagcgg gctctcgtag aagagaccct tgaaatcgat catgcggatc
     2581 cgctgttcgt ccttgcccga cagcaccgac aagaccgcaa aggccccgag aaccgtcact
     2641 ccgtaggtca ccaggtagaa gagggacccc gatatcccat cgtcgttcgc ggcgactacg
     2701 cccgtgagga tgaaccccgc atgagctatc gatgaatagg caagcagtcg tttcaggtcc
     2761 tcctgcacga tcgcgacaac ggacccgacc accatcgtga ccgtggccag aacgaggatc
     2821 aggggctgcc agtcccaccg caacgggaac aagactgcgt ccaagagccg gagcgtggcc
     2881 gcgaacgcgg cgatcttcgt tgctgccgcc atgaatccgg tcacgggggc gggtgcgccc
     2941 tggtacgcgt ccggagtcca cgcgtggaac ggaacggccg caaccttgaa gcccatcccg
     3001 acgatcaaga atcccgtggc gacgaccatc aaggcaaggt cggcggtcgg cgttgagccc
     3061 aggtaacgag agatcgactc ggaaggtcct cctcccgaat agaaggccgt cgttcccgca
     3121 gcgccgtacg cgagcgcgac tccaagcagg aagaaagcag acgagaacgc gcccaacagg
     3181 aagtacttga gtgcagattc ctggctctcg gtcgaacgcc gacggaagcc gacgagtaca
     3241 tagatcgcga gcgagaaggt ctcgagagcg agaaagacga cgatcagatc ggccgctgca
     3301 gccatgagca tcatccccgc cgtcgcgaac aggatgagca cgtagaactc agcgtcctcc
     3361 aaaccctccc gcgcgaggta gtcgcgcgcg agccaaaccg tgagcgtggc gaaaaccgcc
     3421 aagccgatct tcacgaagat cgcgaaccca tccaatgcga tcgcaccccc gagctggacc
     3481 tcgttcgcgc cccacgagga gacggccaag agagtcgcca ccgtcagacc tagggtcgcg
     3541 atgaccggtg aataccagcg cgaatggctg ggcgcgatgc ggactacaaa ggcgtcgaac
     3601 accatcagga agagcgcagt gatcgtcaga gcgatctcgg gcgcgatccc ggcgagctct
     3661 accgcgggag ggtcgaaggg catctagcgg cctccctcgg cagtgacgga atccgaaccg
     3721 gcaggccgcg tgacgagatg gcgttcggcg gacggctcga tgcgatccag gaacggcttc
     3781 gggtagatgc cgatgaagac gatcagcgcg acacatggga cgaccatcgc cagctcgcgc
     3841 ggcgacagat ccggtagctc gcgattctcc ttggatgtcg gctcaccgtg gaagacgcgt
     3901 tggtaagacc gcaacatgta gatcgcggcc agaacgacgc cgaacgcggc cgggatcacc
     3961 caccacccgt agctgaagaa cgttcccaac aggatgagga actcgccgac gaagccgttc
     4021 aaccccggaa gtccgagcga cgacagagcc acgactaaga aaacgcccga gaagatcggg
     4081 atcgacttag ccaggccgcc gaagtcggct atcgctcgtg tgtgccggcg gtcgtagagc
     4141 gcgccgacga gcatgaacag cgcccccgtc gacagcccat ggttgaccat ctggaggatg
     4201 gagccggaca tcgcctcggt gttgccgacg aatacaccca gcacgacgaa gcccaagtgc
     4261 gccaccgacg aatacgcgat caaacgcttg aggtccttct gcatcatcgc taccagggcg
     4321 ccgtagacga tcccgatcat cgccagggcc acgacgagcg gagcgagttg atcggccgcg
     4381 tcggggaaca acgggatccc gaacctgatc agcccataag cacccatctt caagagaacg
     4441 ccggcaagca tcacggagcc ggccgtcgga gcttccgtat gcgcgtccgg aagccacgtg
     4501 tggaacggca ctaacggcac cttgaccgcg aaggatgcga agaaagccag gaacaggagt
     4561 cgctggacct ccagcgacaa cggggtcccc agcagcaccc ggtagtcgaa gctcctcaat
     4621 ccctcgttcg cgaagtagag agccaggatg gcgacgagca tcaagagcga accgacaagt
     4681 gtgaacagga agaacttcat cgccgcatag atccgcttgc tgtagcccca catcccgatc
     4741 aggaaataca tcgggacgag cgtggcctcc cagaacagat agaagaggaa aagatccagc
     4801 gcggcgaaca cacccaacat cccggtctcg agtgccagaa ggaagatgaa atatggcttc
     4861 gcgcgcttgg tgatgctcca cgaagccaga acgccgagtg gcatcaagaa ggtcgtgagc
     4921 aggatcatcc acaagctcac tccatcaacg gccgtcgtgt aaccgacgcc ccattccggt
     4981 atccactgca cggaagtgac caactggaaa ccggcgaccc ccgcctcgaa ctgagcgaga
     5041 acgaagagcg aggtaacgaa cgcgacgagg gtgaccgcta gggccgttag gcgcacggca
     5101 cgaaggttgt ccctcgggac gaagcccagt agcagagcgc cgactaatgg caggaagatc
     5161 gtcaggtcga gcatcgagaa ccgactcatt tgtcgctcat acggcgaccc tgaccacgag
     5221 gatcgagatc agcagaacga ctccgaacaa gaacgtcgcg gcgtagttac gcacgtatcc
     5281 cgtctgcaag ccgcgcaagc tctccgccgc attggcgacg aatcgcccta gtccggtgat
     5341 ggctccgtcg acgtaccggc gatcgaagac gtcggagagg tacgcggcga atctcttacc
     5401 ggggccgacg atcgtggcgc cgtagatctc atctagccag aacttgttct gcgccgcggt
     5461 aacgagtggg cgaacccggc taacgatccg cctgcgcctg aggctcccag cgcgcgtcag
     5521 atacagcgaa cgagcaaaga agatcccggc gacggcgacg agcagtgcga tccctccaag
     5581 aacccagggc gtgaggccgg tcgggacgtg cgcttcgccg accaccggct ccagaaagac
     5641 ctcgaggctg agaacgccgg gcaggttgat catcccgccg gaggccgaca acacaccgag
     5701 cacgaccagt gcgaacgcca tgctcggagg tgcatcgtgg atgtgcttgt catcggcgta
     5761 gcggggccgg cccgcgaaca cccggaaata caaccgggac atgtagaaag cggtgaggaa
     5821 ggccgtcgcc actccgatcc cccacagggc gtactggccc tcggaccaag ccgacgccag
     5881 gatcgcgtcc ttcgagaaga accccgagaa cgggatcagc ccgcagatcg ccaaccagcc
     5941 gagcgcgaac gaactgctgg tcaacggaag gtgatgcctc aacccaccca tcttccgtat
     6001 gtcggtctcg ccaccgagcg cgtgcatgac cgcgccggca ccgaggaaca tcaacgcctt
     6061 gaagaacgcg tgagtcacca gatggaagat gccgactgag taagcaccga cgccgtgagc
     6121 caggaacatg tatccgagct gggagatcgt cgagtacgcg agcacccgct tgatgtcgta
     6181 ttggaccgaa gccatcaggg cggccatcaa tgcggtcagc gctccgatcc aacccacgac
     6241 catcagcgct tcgggaccgg cgtgctcgaa cacgggactg aagcgggcaa ccatgtagac
     6301 gccggcagta accatcgttg ccgcgtggat cagagccgac accggagtgg ggccctccat
     6361 ggcatccggc aaccacacgt acagaggtat ctgagccgac ttaccggtcg caccaccgaa
     6421 caggagcaag gcagcaaccg ttgcgacccc cgcgctcatc gcgccgccct ccgccgacgc
     6481 gttgaccgca tcggccgaaa ggctgccaac ggttcccgcc agcaacaaca tgccgagcaa
     6541 gaacgagaag tcgccgatcc ggttgacgag gaacgccttt ttggcggcgt tgtgggcgga
     6601 gggcttctcg aaccagaaac cgatcagaag gtacgagcac aggcccactc cctcccaccc
     6661 gacgtacaag agaagaaggt tgtcggcgag aacgaggatc aacatcgaag cggcgaagag
     6721 attcagataa gcgaaatagc gcgaaaaacg cgaatcaccg tgcatgtaac cgatcgaata
     6781 gatatggatc aaagtcccca caccggttac aaccagaagc atcacggccg acagtgggtc
     6841 gatccggaga tcgaacgcgg ccgagaagct gccggccttg atccactcga aggccgtgac
     6901 gacgacctct cgttcctcgg cgggcatacc gagcaggttg aagaagagca ctacggcgct
     6961 caggaaggaa cgggcgagag cgatcgaggc cagccatccc gtccagccct tgaccctcat
     7021 accgaagatc gacatcgctt cgagccgcgt tccgaacagc aggagcacga tcgccgtcgc
     7081 cagcgggaag gtgaacgtcg acagcgacaa gaccaggacg tcggagccgg ggatcagttc
     7141 cactagcgcc tcaagagatg ggcgtcgtcg acgttggcgg tcgttcggtg cctgaagatc
     7201 agggtaatga tcgcaagccc aacgactact tcggctgcgg cgacgaccag gacgaagaag
     7261 acgagcactt gtccgtcgat gtggccgtgc atccgggaga aggtcacgaa gaagaggttg
     7321 accgcgttga gcattagctc gatgaacatg aagatgacga tcgcgttccg tcgcaccagc
     7381 gctccgacga ctccgacggt gaacatcaga gcagcgagca tcaggtagaa gcccggagcg
     7441 acgctcactc ggcggcacca tcctggccga cggcgctgag acggcgcgcc agcaccatga
     7501 ctcccacgat cgcaacgacg aggagcacag aggtggcctc gaacggcaac aggtagcggc
     7561 tgaataaggc ccgcgcgatc gattcgacgt tgccgggatc ccggttcagt gccgtcaatg
     7621 cttctgcacc ccgcctcgcc tccgagatcg cacccacacc tcgccacgcg acgtaagcga
     7681 tctgtgccgc gagcaacgct ccaagtccga tcgcgagcga ccgctggccg ggcaggggtt
     7741 cgatcaaagc ttcacgacga tcgacaccaa gaagcatgat cacgaacagg aacagcacca
     7801 tgatcgctcc ggcgtagacg atgatctgga tcgcggcgat gaagaaagcc ccttgcagca
     7861 ggaatgcaac ggcgagcgct atctgagcgg caacaaggaa gagggccgtg tgaacggcgt
     7921 tgcgagcgaa caacatcgcc agcgacgacc cgagggccac gatgaacatc accccgaaca
     7981 ggatctgctc gaggttcatg aggagtcctt gcgaccggac ttgatgacgt cgaagttgtg
     8041 ctcgggttcg agctccttct tggtaggcca gtgccggact atcggagcat cgacgatcaa
     8101 ctgctccttc gtgaagatga gctcttcacg tgaggatccc gagatctcgt actcggtcga
     8161 catcaacagc gcctcggtcg gacacgcctc gacgcacaat ccgcagaaga tgcaccgcag
     8221 gaagttgatc tgatagatct gggcatagcg ttcgccgggc gagaaccgct gctctggagt
     8281 gttctcggcc cccacgacgt agatcgcatc ggccggacaa gctcccgcgc atagctcgca
     8341 accgatgcat ttctcgagcc cgtcctcgtg acggtcgagt atgtgccggc ccttgaatcg
     8401 cgggaacggt cgtctcctta cctcgggata ggcgatcgtc tctttccgtt tgaagacatt
     8461 cgcgatgaca accttcatcc cccggaagat ctcaggcagc gccatggacg gcctccttcg
     8521 cgggcttagg agtaaccgct tccttcaatc gtggatcgcc ccggctgatc cacaccatga
     8581 cgatcatgaa gagtgctgcg aaggtgccga tccgcacgcc gcgaccgatc ccatcggtat
     8641 tgaccactgc agtcacgagg atccagccga gcgatgccgg gatcagtcgc ttccatccca
     8701 accacatcag acggtcgtag cgaagccgag gcaaggtggc gcgcagccac acgaacagat
     8761 agatgaacgc aaccgtcttg agcgtgaacc agaggagggg ccacacccac ggcaagaagc
     8821 cgaaactcgg tccgtgccac cctccgaaga aaagcgtgac ggccatggcc gagacggcaa
     8881 ccacgtggat gtactcaccg aggaagaaca tcgcgaaccg gatccccgag tactcggtat
     8941 ggaagccggc gaccaactcg gtttcggcct ccggtagatc gaagggcgcc cggttcgtct
     9001 ctgcgatccc cgccaccatg aagatcagga aggccggcca ctgcgaccag atgtaccaat
     9061 cgggtgcgta cttgaagatc tgggagacct gttcgagcac ggggatgccg tcgaagaacg
     9121 tcacgtttcc gactgtgccg gcctgcgcgg caacgatgtc gaccgtggag aggctgccgg
     9181 ccacgagagc gataggcacc agagacaagc cgagggcgat ctcgtacgag atcatctgcg
     9241 cgctcgatcg gatcgcgccc agcaacgggt atttcgagcc cgatgcccag cccgcgagga
     9301 cgacgccgta gacaccgagc gatcccatcg cgaggaaata caggatcccg atgttcaggt
     9361 cgctgacgat caacgagatc ttctcgccgc ccacagtgat gcggtcgccg atgggaacga
     9421 ccgcgaacgc gaggaaggcc ggaaccatcg cggcgaccgg cgcgatggca taggtccact
     9481 tgtcggcact tgccggacgg aagtcctctt tgaagaagag cttgatcccg tccgccagcg
     9541 tttgtagtag gccgaaggga ccccagcggt tcggcccgat cctcccctgc atgtcggcga
     9601 tgaccttgcg ctcgaaccag atcacgagga tcgttacgac cagcagcaac aggaagatca
     9661 cgatgacctt caccgccgcg atgatcacgt tcacaccgat gaactcgatc acgcaggcct
     9721 cacctcgaca gtggggtcga tcccacgcat cagagtgttc gcgcacaacc cggcctggtc
     9781 gtaaggaacg aaaaccgctc cgcgaacgat gtcgtcgatg cgcaccggca gttcgacttc
     9841 gacatcgccc gcaatgactc gaaccatgtc gccgtccgcc acaccgagcg ctttcgcgtc
     9901 ctcgtcattc atttcaacga acggtttctt ttgaaggccg tgtaacgcac gggacttaga
     9961 aaccatcgag ccctcgtcgt agatgaagcg gccgctgtag agatcaaacg ttgaacccga
    10021 cggcgaggac gaacttgggt ccgacggcga ctcagaacgg tttggagccg tcgggcgcaa
    10081 gtcgcccgca gccgcccgtg actcctgagc agccgccgga tcgccgtcct cgggttggtg
    10141 ctgatctggg atctccggga cgtagcccgg gcgttgtctt gcctcgaatg ctcttagttc
    10201 ccaggacagc ggccaggcgt tgccggtttg gaagggggct ccggagcggt agcccttggg
    10261 gtattgccca cccggtccag caacagatgt ctgcgcttca tgcgacttcg acgcgaaacc
    10321 ggtttcgtat tgcagggagt ggacgggcat ctcttgggtt agcgcggcgg tttcgacctg
    10381 tgcatgcgta ggcacgttcg agcggatgtc atcccacacg tcgtcgcatc cctgccagcc
    10441 ccagtccgcg tcgaggccgc gagcgatacc ggcagcgacg cgccagggct ctcgcgcggt
    10501 gccgggtgga gccagcaacg gctccagttt ctgaagcctt cgttccaagt tcgtgaaggt
    10561 tccctcgcgc tcagcgtagg ccgcacttgg gaggacgacg tcggcgtgct gaacggtttc
    10621 ggtcgggaac agctcgacga ccagcgcgaa cttcaccgac agtagagctc ggcgcgcaag
    10681 ctcggcgtcg gggaagtccc ggatcggatc ggcaccgaag atgagcaaaa cgtcgatctc
    10741 gccggcgact gccgcttcga ggatcgcgcg ggtgtctcga cccgtctcgt cggtggaacc
    10801 gagagcggga tgagcaccca tatccagtag accctgcgac cccgcgtgcg gaggacagac
    10861 gagcactttc gcatccgtcc tctgtgcaag gttcagagcg gccgacgccc acgcgctttc
    10921 atcgcggccc ggagaggacg gaccccacgc gacgacgacc ggcccttcga tgtcgccgag
    10981 cgcatcgagg tctgc
//
LOCUS       DATMED010000394         6805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_325945, whole genome shotgun sequence.
ACCESSION   DATMED010000394 DATMED010000000
VERSION     DATMED010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325945"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2220
                     /gene="mfd"
                     /locus_tag="VNP73_10275"
     CDS             <1..2220
                     /gene="mfd"
                     /locus_tag="VNP73_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWL66347.1"
                     /translation="LHKNLKGMGLDYPVTDAAPNASSPVGGSIVVADLGRGFVLGPPT
                     CLALVAESDLTGRRSGAARKRLQARRRSTTGPLELAPGDLVVHEVHGIGRYVEMVERE
                     LLGVHREYLIIEYARGDSLFVPSDQVDLVSKYIGGEAPKLNRLGSSEWGKAKSRVRRR
                     TREIAQDLVKLYSERLRAKGHAFGPDTPWQRELEDSFPYEETPDQLRAVDEVKDDMEQ
                     DTPMDRLVCGDVGYGKTEIALRAAFKAVAEQKQVAVLVPTTILAQQHHATFVERFRHF
                     PVRVEMLSRFLSAAESKKVIADLAAGKVDVVIGTHRLLQKDVSFADLGLVIVDEEQRF
                     GVEQKEKLKSLRASVDILTLTATPIPRTLEMAMAGIRDMSIVDTPPEDRHPVMTYVGD
                     FSEEVVSSAVRRELLRDGQVFYVYPRVQTIDHTARRLMELVPEARVGVAHGQMDEKIL
                     EQAMIDFTDAKTNVLVCTTIIESGLDIPTVNTLIVERSDLLGLSQMYQLRGRVGRAHE
                     RAYAYLFFPPERSLTEEAHERLKTIAEHTGLGSGFRIAMRDLEIRGAGNLLGAEQHGH
                     IAEVGFDLYVKLMASAVDEAKGMPWREDGEVRIDLPLDAFIPKDYIADENLRIEAYRR
                     IAAARDEEDLTEVRAELEDRYGAPIPDSVGSLFDVVRLRVLMADRGIVEAATVAKNLR
                     IRPIELTESKEIRLQRILPDAQWRATTQTLLIPERSIPEQGVVTWVSDVVQQLTSPA"
     gene            2217..3251
                     /locus_tag="VNP73_10280"
     CDS             2217..3251
                     /locus_tag="VNP73_10280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWL66348.1"
                     /translation="MTLRTGALVCAFLFLTGCAQLLEPAAAVVYGRKIPVDEVNEGLD
                     RFVATGEFERLSAQGDSQAIKRQFEQGFLSQLIRRSVLQPKAEELDVEITDQELDARL
                     DEIRNDFPSPAAFQEALKEQGLEEDQLEQLVGDSLLEEELKAEVTERAAPSETELRDY
                     YETRIEDYEQTRAQHVLVENRALADEIAGQAASVPKKNLDKLFARLAKEFSIDNSNAK
                     EGGDLGYFAAGDFVAEFENAAAKLDVGEVSGPVRSEFGFHIIWVTDRRVQAFEDVRPE
                     LEQRLAAENEDRVWSDWLTAAYVDAEVKVNPRYGELDVESGQVVEASAEDIPGAEEPR
                     ESPTPSVPGG"
     gene            3264..4223
                     /locus_tag="VNP73_10285"
     CDS             3264..4223
                     /locus_tag="VNP73_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HWL66349.1"
                     /translation="MPLIVVPLAVDQVGLLTLDEWDALTSCTRVLFERPDHPLVERLR
                     AAGVAAGPFDDDPDAGREGWALVTDPDSPRVIELARNGASVTSGAAPAPDAPTAAHGA
                     YVARRAQAELGGLALIMARLRSEDGCPWDREQSHESLRVHLVEEAHEVLEAIDRGHVG
                     AELEEELGDLLLQVAFHAQMAADDGRFDLAGVAHGISSKLIHRHPHVFGDTEVSGAAE
                     VVRNWESIKAQEKDRSDPFEGIPASLSALLHAYKTQKRAAGKGVEITGADAQAHVLQA
                     LGQETDARSLGEALFWLVAVARSAGIDPEGALRSATGRFRDSL"
     gene            4280..5566
                     /gene="eno"
                     /locus_tag="VNP73_10290"
     CDS             4280..5566
                     /gene="eno"
                     /locus_tag="VNP73_10290"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWL66350.1"
                     /translation="MSEIVDVVGREILDSRGNPTVEAEVVLDSGARGRAGVPSGASTG
                     SNEALELRDTEDKRYGGKGVRTAIGHISDVIAPALLGRDALDQRGVDTLMRDLDGTGD
                     KSKLGANAVLGVSLAVAHAAAIELDLPLFRYLGGPDAHLLPVPLLNVLNGGAHADNSV
                     DLQEFMLAPIGAASFSEALEMGATTYHQLKKTLDAKGMSTGVGDEGGFAPDLSANEEA
                     VQLLIDAIEAAGYTPGTDIALALDPATTEILEGDEYVIASENRRLSRDEMVAFWIDWV
                     DRFPIVSIEDGMAEDDWEGWGLLTEAVGGKVQLVGDDLFVTNPAILSRGIDEGVGNAI
                     LVKPNQIGSLTETLECIRIAQRAAYATVISHRSGETEDATIADLAVATNAGQIKTGAP
                     ARGERTAKYNQLLRIEDLLGDAARYGGARAFPRWKA"
     gene            5563..5976
                     /locus_tag="VNP73_10295"
     CDS             5563..5976
                     /locus_tag="VNP73_10295"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWL66351.1"
                     /translation="MKAVVARPRLRMPRLGVGPQVVVVLLVLSLFGAMAIEPTRQLMS
                     QRARIAGVAQDLQQIERSNDRLAGRIDRLQDPDFLEQRAREIGLVRPGEDSIVVMPPS
                     KKMQDHKKPDKKRPKSDKVRPLGFFEGFLNFVGII"
     gene            6054..6410
                     /locus_tag="VNP73_10300"
     CDS             6054..6410
                     /locus_tag="VNP73_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWL66352.1"
                     /translation="MVDVGAVVEGTVEKVTEYGAFVKLESGESGMVHISQVDVNYVRN
                     ISDFIREGDKVTVKVIGQKDDGKIDLSIKQAQAGYEERPRRGGHDPEFEQKLKRFMSQ
                     SQERLVDLKRHQEGKR"
     gene            6506..>6805
                     /locus_tag="VNP73_10305"
     CDS             6506..>6805
                     /locus_tag="VNP73_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HWL66353.1"
                     /translation="MGTRCHLGIPMVIESHPVLDDGSPFPTLYWLTCPVLSKSVSTLE
                     SDGWMAGVNSRLASDGSLRARVAGAIDRYRARRDARAEISDGGSATGGGPDRVKCV"
BASE COUNT         1299 a         2075 c         2238 g         1193 t
ORIGIN      
        1 ctgcacaaga acctcaaggg gatggggctc gactatccgg taaccgacgc ggcccccaac
       61 gcgagctcgc ccgtcggggg ctcgatcgtc gtggcggacc tgggccgagg cttcgttctg
      121 ggcccgccca cctgtctcgc actggttgcc gaatccgatc tgaccggacg ccgctcgggc
      181 gccgcccgga agcgcttgca ggctcgtcgc agaagcacca cggggccgct cgagctggca
      241 ccgggggatc tggtcgtcca tgaggtgcac gggatcggcc gctacgtcga gatggtcgag
      301 agggagctac tgggtgttca ccgcgagtac ctcatcatcg aatacgcgcg cggcgacagc
      361 ctcttcgtgc ccagcgatca ggtcgacctc gtttcgaagt acatcggtgg tgaagcaccc
      421 aagctgaatc gactcggctc gtccgaatgg ggaaaggcga agtctcgggt ccgacggcga
      481 acgcgcgaga tcgcgcaaga tctcgtgaag ctctactcgg aacgtttgcg tgccaaaggc
      541 cacgcgttcg gccccgacac accctggcag cgcgaacttg aggactcgtt cccttacgag
      601 gagactcccg accaactgcg ggccgtcgac gaggtaaagg acgacatgga gcaagacacg
      661 cccatggatc gcctcgtgtg cggcgatgtc ggctacggca agaccgagat cgcactgagg
      721 gctgcattca aggccgtcgc cgagcagaag caagtggcgg tcctggttcc gacgacgatc
      781 cttgcgcagc agcatcacgc cacgttcgtg gagcggttcc gccacttccc cgtgagggtc
      841 gagatgttgt cgcggttcct ctccgcggcc gagagcaaga aagtcatagc cgatctcgcc
      901 gccggcaaag tcgatgtcgt aatcggtacg caccgacttc ttcagaagga tgtgtccttc
      961 gccgacctcg gcctcgtgat cgtcgacgag gagcaacgct tcggcgtcga acagaaggag
     1021 aagctcaagt ctcttcgggc cagtgtcgac atcctgaccc tcacggccac tccgatccct
     1081 cgaacgctcg agatggcgat ggccgggatc cgggatatgt cgatagtcga cacccctccc
     1141 gaggacagac atcccgtgat gacctacgtg ggggatttct cggaagaggt cgtgtcttcc
     1201 gcggtccggc gcgagctatt gcgggacgga caggtcttct acgtctaccc gcgcgttcaa
     1261 acgatcgatc acaccgctcg ccgtctgatg gagctcgtgc cggaggcacg cgtcggcgtt
     1321 gctcacggtc agatggacga gaagatcctg gagcaggcga tgatcgactt cacggatgcg
     1381 aagacgaacg ttctcgtctg cacgacgatc atcgaatcgg ggttggacat ccccaccgtg
     1441 aatacgttga tcgtagaacg atccgatctg ctcgggctgt cgcagatgta ccagttgcgc
     1501 ggccgcgtcg gacgcgccca cgaacgggcc tacgcgtacc tcttcttccc gcccgaacga
     1561 tccctcaccg aggaagcgca cgaacgcttg aagacgatcg ccgaacacac tggacttggt
     1621 tccggattcc ggatcgcgat gcgcgacctc gagatccgag gggcagggaa cctattgggg
     1681 gccgagcaac acggtcacat cgccgaggtg ggcttcgatc tctacgtgaa gctgatggcg
     1741 tcggccgtcg acgaggcgaa ggggatgccg tggcgcgagg acggcgaggt ccggatcgat
     1801 ctgccgctgg acgcgttcat cccgaaggac tacatcgccg acgagaatct gcgcatcgag
     1861 gcttaccggc gcatcgcggc cgctcgagat gaagaggacc tcaccgaggt tcgcgccgag
     1921 ctcgaggatc gctacggcgc acccatcccc gactccgtcg gatctctatt cgacgtcgtg
     1981 cggctgaggg tcctgatggc cgacaggggc atcgtcgagg cagcgaccgt tgcgaagaac
     2041 ctgaggatcc gtccgataga actgaccgag tcgaaggaga tcaggcttca aaggatcctc
     2101 cccgacgcgc agtggagggc aaccacccaa acgctgctga tacccgagcg gtcgatacca
     2161 gagcaaggcg tggtgacgtg ggtgagcgac gttgttcaac agttaacctc gcccgcatga
     2221 ctcttcgcac gggcgccctt gtgtgcgcgt tcttgttcct caccggctgc gcgcaactgc
     2281 tcgagccggc cgcggcggtc gtctacgggc ggaagatccc cgtcgacgag gtgaacgagg
     2341 gtctggacag gttcgtcgcc accggagagt tcgagcgctt gtcggctcaa ggggactcgc
     2401 aggcgatcaa gaggcagttc gagcaggggt tcctctctca actgatacgt cgttcggtcc
     2461 tgcagccgaa ggcggaggag ctcgacgtcg agatcacgga tcaggaactc gacgcacgac
     2521 tcgacgagat ccgtaacgat ttcccgtcac cggccgcgtt ccaagaggcg ctcaaggagc
     2581 aaggcctcga agaggatcag ctcgagcaac tggtcggtga cagcctcttg gaagaggaat
     2641 taaaggcaga ggtcacggaa cgcgcggcac cttccgagac ggagctgcgc gattactacg
     2701 aaacgcggat cgaggattac gagcaaacgc gcgcgcagca cgtccttgtg gaaaacaggg
     2761 ctcttgccga cgagatcgcg ggacaagcgg catccgttcc caagaagaac ctcgacaagc
     2821 tgttcgctcg tctggcgaaa gagttctcga tcgacaactc gaacgcgaag gagggcggcg
     2881 accttgggta tttcgcagcg ggggacttcg ttgccgaatt cgagaatgcc gccgccaagc
     2941 tcgacgtcgg cgaggtatct ggaccggttc gatcggagtt cggtttccac atcatctggg
     3001 tcacggatcg gcgcgtacag gcgttcgagg acgttcgtcc ggagttggaa caaaggttgg
     3061 cggccgagaa cgaagatcgt gtctggtccg attggttgac ggccgcatac gtagatgcag
     3121 aggtcaaggt caatcctcgc tacggcgagc tcgatgtcga gtccggccag gtggtcgagg
     3181 cttcggccga ggacatcccg ggcgccgagg aaccgcgcga gtccccaacg ccttccgttc
     3241 ccggtggctg atcctccact cagatgcccc tgatcgtcgt gcccctcgcg gtcgaccagg
     3301 ttgggctgct cacgcttgac gagtgggacg cgttgacgtc atgcactcgc gttctgttcg
     3361 agcggcccga ccatccactt gtcgaacgtt tgagggcagc gggtgttgcc gccggacctt
     3421 tcgacgacga tcccgacgcc ggccgcgagg gttgggccct cgttacagac cccgactccc
     3481 ctcgggtgat cgagctcgcc cgcaacggcg ccagtgtcac gtcgggtgcc gcgccggctc
     3541 ccgacgcgcc cacggccgct cacggtgcct atgtcgcgcg tcgcgctcaa gcggagctgg
     3601 ggggtctcgc gttgatcatg gcccggctcc gcagcgagga cggatgtcca tgggataggg
     3661 aacagtcaca cgagtcgctt cgcgtccatc tggtcgagga ggcgcacgag gttctcgagg
     3721 cgatcgatcg aggccatgtc ggcgccgaac tcgaggagga gctgggcgat ctcctgcttc
     3781 aagtggcttt ccacgcgcag atggccgccg acgatggacg ctttgacctc gcgggcgtgg
     3841 ctcatgggat ctcgtccaaa ctcatccacc gccatccgca cgtgttcggc gataccgagg
     3901 tctcgggtgc ggcggaggtg gtccgcaact gggaatcgat caaggcacag gagaaggacc
     3961 ggtccgatcc gttcgaaggc atccccgcgt ctctgtctgc cctgcttcac gcgtacaaga
     4021 ctcagaaacg cgcagccggc aagggggtcg agatcaccgg agccgacgcg caggcccacg
     4081 tcctgcaagc tttgggacag gagacggacg cgcggagttt gggtgaggcg ctcttctggc
     4141 tcgttgccgt tgctcgctct gccggcatcg atccggaggg cgcgctcaga agcgcaaccg
     4201 gtcggttccg ggattcgctg tgatgcgact gctaatctgc ctgttactga tgttgaagga
     4261 gtaaacgatt ggttaacgag tgagtgagat cgtcgatgtc gtcggccggg agatcctcga
     4321 ctctcgaggc aatcccacgg tcgaagccga ggtcgtcctg gattcgggtg cccgtggccg
     4381 tgccggcgtg ccctcgggag cgtccacggg atccaacgag gctctcgagc tccgggacac
     4441 cgaggacaag aggtacgggg gcaagggggt ccgaactgcg atcggacaca tctccgacgt
     4501 catcgcaccg gcgctgctcg gtcgcgatgc cctcgaccag cgcggcgtcg acactctcat
     4561 gcgggatctc gacggaacgg gagataagtc caagttgggc gccaacgccg tcctcggcgt
     4621 gtcgctggcg gtcgcgcacg cggcggcgat cgagctcgac ctcccactgt tccgctactt
     4681 gggggggccg gacgcgcacc tcctaccggt gccacttctc aacgttctga acgggggcgc
     4741 gcacgcggac aactcggtcg acctccagga gttcatgctt gctccgatag gagccgcatc
     4801 cttctccgaa gctctcgaga tgggcgcaac cacctatcac cagttgaaga agaccctcga
     4861 cgccaaaggg atgtcgactg gcgtgggaga cgaaggtggg ttcgctcccg acctgtcggc
     4921 gaacgaagaa gcagttcagt tgctgatcga tgcgatcgag gccgccggct atacgccggg
     4981 gaccgacatc gccctggctc tggaccctgc gacgactgag atcttggagg gtgacgaata
     5041 cgtcatcgct tccgagaacc gacggttgtc ccgtgacgag atggttgcct tctggatcga
     5101 ctgggtcgac cggttcccca tcgtgtcgat cgaggatggg atggccgagg acgactggga
     5161 gggttggggt ctccttacgg aagccgttgg gggcaaggtc cagctcgtgg gcgacgatct
     5221 cttcgtcacg aaccctgcca tcttgtcgag aggcatcgac gagggcgtcg gtaacgcgat
     5281 cctggtcaaa ccgaaccaga tcggaagcct taccgaaacg ctcgagtgca tccgcatcgc
     5341 gcaacgcgct gcttacgcca cggtcatctc acaccggtcg ggtgagacgg aagacgcgac
     5401 gatcgcggat cttgccgtcg ctaccaacgc gggacagatc aagacgggtg ctccggcgcg
     5461 gggagaacga accgctaagt acaaccagtt gctgcgcatc gaagatctgt tgggcgacgc
     5521 ggctcggtac ggtggcgctc gcgcgttccc gcggtggaag gcatgaaggc cgtagtggcc
     5581 cggccccggc tgcggatgcc gcgactcggc gtcggccctc aggtagtggt cgtcttgctg
     5641 gtgctgtccc tgttcggcgc catggccatc gaaccaacca ggcaactcat gtcgcaacgc
     5701 gcccggatcg cgggggtggc ccaggacctc cagcagatcg agcgctcgaa cgaccgcctt
     5761 gccggccgca tagaccgcct ccaggacccc gatttcctgg agcagagggc gcgggagatc
     5821 ggcttggttc gcccgggcga ggactcgatc gtggtcatgc cgcccagtaa gaagatgcag
     5881 gaccacaaga agcccgacaa gaaacgaccc aagagcgaca aagttcgacc gctcggcttc
     5941 ttcgagggtt tcctgaactt cgtcggcatc atctagccat tcgggcgtca cgcttcccgt
     6001 accccagacg acggggttaa agtgactgac atgaacgttc tccagggggg acattggtag
     6061 acgtaggcgc ggtcgtcgag gggacggtcg agaaggtcac cgagtacggg gccttcgtga
     6121 aactcgaatc cggcgagagc ggcatggtcc acatctcaca agtggacgtg aattacgttc
     6181 gcaatatctc ggacttcatc cgtgaaggcg acaaggtgac ggtcaaggtc atcggccaga
     6241 aggacgatgg caagatcgac ctttcgatca aacaggcaca ggccggttac gaagagcggc
     6301 ctcgccgcgg cggccacgat cccgagttcg agcagaagct caagcgattc atgagccaaa
     6361 gccaggaacg tctggtcgat ctgaaacgtc accaggaagg gaagcggtag aggcgcccgc
     6421 tcgccccgac gcgctcgatc actcgagcgc cgatctctcc atcgtcaccc agcagctcgg
     6481 tcgaactccg cgcggccggt gggcagtggg gactcgatgt cacctcggga tccccatggt
     6541 catcgagagc catcccgttc tggacgacgg ttcaccgttt ccaaccctct actggctcac
     6601 ctgtcccgtg ctatcgaaaa gcgtcagcac gttggagtcg gacgggtgga tggcaggggt
     6661 caacagccga ttggcttcgg acggctcgtt gcgtgctcga gtggccggtg cgatcgatcg
     6721 ctaccgcgcc cgccgtgatg cacgcgccga gatatcggac ggtggctcgg caaccggtgg
     6781 agggccggat cgtgtgaagt gcgtg
//
LOCUS       DATMED010000395         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3010240, whole genome shotgun sequence.
ACCESSION   DATMED010000395 DATMED010000000
VERSION     DATMED010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010240"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..964)
                     /locus_tag="VNP73_10310"
     CDS             complement(101..964)
                     /locus_tag="VNP73_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWL66354.1"
                     /translation="MELELRQLRAFVAVVDEGSFTKAAGVLLTSQASVSRAISALEKA
                     VGAPLLERTTRQLSLTSTGARIVGHARRVLEEAAAIHRVAVESPSEIRVGYAWGALGR
                     HTITAQQRWKATHPGSEIRFVQSRSPTAGLAEGVAEIAIVRRPLTDERFTTTLIGVEH
                     RYVALSSSDPLARRRNLTMNDLTGRTIALDPLTGTTTPDLWPDDAGPRETREVVGVED
                     WLTLIAGGQAIGMSSEATAQQHLRPGVVYRPVRDAQPIPVWLAWWRDSPPRNASALVQ
                     LVCDLYRTDRS"
     gene            1034..1984
                     /locus_tag="VNP73_10315"
     CDS             1034..1984
                     /locus_tag="VNP73_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007380514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWL66355.1"
                     /translation="MEIETKQPRVPSGGRGSPSLARLATMLGSGISNQVGAAAGAHAF
                     SAIGPAGVVAMRQIVAAAVLLPIARPSIRRMTWPQWWPTLLLAVVFATMNLSLYIAID
                     RIGLGLAVTLEFLGPLGVALASSRTRVHLLAAISAAVGVYVLVLPGPSSDYVGIGLAL
                     IAAASWAAYILLNRTVGARLPGLEAPAIATSLSALLYLPVVVTLTAQGRLNGLPLFYA
                     ITAGVLSSVVPYAADLIVLRHVPARFFGTFMSMYPVWAALAGLILLGQVPAIHVWIGI
                     AVIALSNVVAVAAAHGSEARGVTTGVSSRPGLATAGAETC"
BASE COUNT          401 a          739 c          729 g          459 t
ORIGIN      
        1 cgcgcgcatg gcgcgcgagc tgagtcacaa cgcgaaggaa ggggctgggt gagctccaag
       61 ccgacgcgcc gatgtgttcc acatttgttc cacgttcgct ctagctgcga tcggtcctgt
      121 agagatcaca gacgagttgg accagcgccg atgcgttgcg cggaggactg tcgcgccacc
      181 acgccagcca cacagggatc ggctgagcgt cacgtactgg gcggtagacc acgccaggac
      241 gcaaatgctg ttgggcggtc gcctcagagg acatgccgat ggcttgaccg ccggcgatca
      301 gcgtgagcca atcctcgacg ccgaccacct cgcgcgtttc tctcggccct gcgtcatccg
      361 gccataggtc gggcgtggtt gtgccggtca gcgggtccag cgcgatcgtt cgccccgtga
      421 ggtcgttcat cgtgagatta cgccgacgag ccaggggatc cgagctcgac agagcgacat
      481 agcgatgctc gaccccgatg agcgtcgtgg tgaagcgctc gtccgtcagt gggcgacgca
      541 cgatggcaat ctccgccaca ccctccgcca gccccgccgt cgggctcctc gattgaacga
      601 agcggatctc tgaaccggga tgcgtggcct tccaccgctg ctgcgcggtg atggtgtggc
      661 ggcccaatgc tccccacgcg tagccaacgc ggatctcaga cggggattcg accgcgactc
      721 ggtggatcgc cgcggcctcc tccaggaccc gccgggcgtg acccactatc cgtgcgccgg
      781 tcgaagtcaa tgagagctgc cgggtcgtgc gttcgagaag aggcgcacca actgccttct
      841 cgagtgcgct gatcgcgcgg gaaacagatg cctgggacgt cagcagcaca cccgctgcct
      901 tcgtgaaact gccctcgtcg acgacagcca cgaatgcccg tagctgcctc aattcaagtt
      961 ccatacggat aacgtatcag tcgttgcaat attcgcgatt gacgcatgaa gcttaggggc
     1021 ggacgctggt gtcatggaga tcgagacgaa acagcccagg gtgcctagtg gcggccgggg
     1081 cagcccttcc ctagcccggc tggcgacgat gttgggcagt gggatcagca accaggtagg
     1141 cgctgccgct ggggctcacg ccttcagcgc catcggacct gccggggtcg tcgcaatgcg
     1201 ccagatcgtg gcagccgcgg tccttctgcc gatagctcga ccatcgatac gcaggatgac
     1261 atggccccag tggtggccca cgcttctgtt ggcggtcgtg ttcgcaacga tgaacctctc
     1321 cctgtacatc gcgatcgatc ggatcggcct tggcctggct gtcactttgg agtttctcgg
     1381 tccgctcggc gtcgcactag cgagctctcg cacccgagtg catctcttgg cggccatctc
     1441 ggccgccgtg ggtgtctatg tgctggtcct cccaggtcct tcgagcgact acgtcgggat
     1501 cggcctggca ctaattgccg ctgcatcttg ggctgcctac atccttctga atcgaactgt
     1561 cggcgcgagg cttccgggcc tcgaggcgcc agccatagca acatcgctgt cggcgctgct
     1621 gtatctccct gtcgttgtta cgttgactgc ccagggccgg ctgaacgggc tgcctctgtt
     1681 ctacgcgatc accgcgggtg ttctttcttc ggtggtgcct tacgccgccg acctaatcgt
     1741 tctgagacac gtgccggctc gcttctttgg aacattcatg agcatgtatc cggtatgggc
     1801 agctcttgca gggctcattc tgctaggtca ggttccggcc atacacgtct ggatagggat
     1861 agcggtgatc gcgctgtcca acgtcgtagc cgtcgcagct gctcacggaa gtgaggctcg
     1921 cggcgtgaca acgggagtct cgtcgcgtcc cggcttggca acggcgggag cggagacttg
     1981 ttgatgcatc attggtgcca tgacaggatc ttgtgcgctg acgtcgtacc agcggacgag
     2041 caagggacac gtggaagggc aatccttcgt gtacgtctga tccagctcca ccatggacgc
     2101 cgggacatct aaggcatcga tttgctcgct gcggtcttcc atcgatccca aacgagcttc
     2161 actacccgcc gccgcgaaca gaaggatcgc caaactcaac cacttggagc gccagactca
     2221 ccagatgcta gctcggctag agcgcttggg ccacgcagaa ttcgttcccc tcggggtcga
     2281 ggagaaccgt ccaccggtag ccccactcgt ccatgtccgc gactcgag
//
LOCUS       DATMED010000396        13746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2255137, whole genome shotgun sequence.
ACCESSION   DATMED010000396 DATMED010000000
VERSION     DATMED010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255137"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VNP73_10320"
     CDS             complement(<1..113)
                     /locus_tag="VNP73_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWL66356.1"
                     /translation="MNLPGGGELIIILLVLLLLFGATRLPKLARSMGQAGK"
     gene            complement(110..1144)
                     /locus_tag="VNP73_10325"
     CDS             complement(110..1144)
                     /locus_tag="VNP73_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWL66357.1"
                     /translation="MKLGLALPHYDTSLAGQPVSWEGVSRIARTAERVGFDSLWVSDH
                     FFLDWSKYGGPDTVQGTFECWTLTSALAAITDRVRVGSMVACNDFRPPALVAKMVASL
                     DELSGGRVDLGLGAGWYEPEYRAAGIEFHNARTRIARLEEAVEIIGRLLDGEELVFKG
                     DHYTIDGAIVRPLGPRSPRPPIWVGGKGDRTLGAIARKADGWNFSWMGPFSTYEERAA
                     AADKACEEADRDPRTLRRSVGVYMLAGTSEADVRRRFERLVERTPPGILRSGGGATGV
                     SWDEFKQDRVAGTVDEVVDRLSRLAELGVEEVVAGLGAIPFQLADEEDVELFGSEIAP
                     ALRELNGGTS"
     gene            1167..1631
                     /locus_tag="VNP73_10330"
     CDS             1167..1631
                     /locus_tag="VNP73_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWL66358.1"
                     /translation="MKVSDIMTAACLSDSPDDTVAEAATKMRQNQTGSILVMDSGKLA
                     GILTERDVLRVVAEGKDPKSAVVKDEMTDNPVTIPTHTSLKDAAQTMFDKWFRHLPVV
                     TNEGDVVGIISLRDLLTIVAESLDEPEAIQSLTGHKLVRDRRLERVEAGDLD"
     gene            1606..1962
                     /locus_tag="VNP73_10335"
     CDS             1606..1962
                     /locus_tag="VNP73_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66359.1"
                     /translation="MSKPATSTDPIPGPRSPHFIRAWSFGLLSIASPFLVPFVARLIQ
                     PGAEVGRIAYLSALGAAAAVSLLGVIFAVLTQRQTAPKTLQRRIAGITGALSGVMLLF
                     WFVSTLALLKLSPSWR"
     gene            complement(2019..3194)
                     /locus_tag="VNP73_10340"
     CDS             complement(2019..3194)
                     /locus_tag="VNP73_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWL66360.1"
                     /translation="MIELNDTQKALVEAVRDFVEKEVIPIADDLEHKDEYPEQIVEQM
                     KEMGLFGITVPEEYDGIGESLITYALCVVELARGWMSLSGVLNTHFMGCYLINKFGTD
                     EQKKNYLPRMATGEIRAALAMSEPEAGSDVQAIRTKAIKEGDEYVVNGQKMWLTNGLR
                     AGIVMTLVKTDPNADPPHSGMSMLIVEKTPGEPKPAAGLDVGKNIEKMGYKGVETVEM
                     TFSDYRCSTSQLLGTEEGMGFKQVLDGIEVGRVNVAARAVGVSTRAFEEAIKYAQERK
                     AFGKQIADHQAVLHHIAEMGTTLEAAKLMLYSAAQKKVAGERSDLEAGMAKLFASEAC
                     HEIVTKSLRVHGGYGYSKEFTVERLYRDAPFLLIGEGTSEIQKNVIGRRLLERYKLT"
     gene            complement(3194..4156)
                     /locus_tag="VNP73_10345"
     CDS             complement(3194..4156)
                     /locus_tag="VNP73_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018254348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWL66361.1"
                     /translation="MAVARARRSCLSVPGSSPKMLSKGPTLPADEVFMDLEDSVAPLA
                     KEEARGNVIDALKNNDWADKTVVVRINSIDTQWAADDLKTIVENAGQYLDCVMIPKVQ
                     YAHEVMFVDHMLRMIETTVGLEKRIGLEVQIETATGLMNIHEIAHASDRMETLIFGPA
                     DMSASLGLPTVTAGLPMPGYPGDHFHWVLETILVAARDAGLQAIDGPYLLIKDVDGYR
                     DMAMRARALGYDGKWALHPGQIGVANEVFTPTQEEFDKAQALLDAYKHATEVDRTGAV
                     MFGNEMIDEASRKMAVQFAERGRAAGLKPNKTLADFEKEWAEKS"
     gene            complement(4186..5766)
                     /locus_tag="VNP73_10350"
     CDS             complement(4186..5766)
                     /locus_tag="VNP73_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL66362.1"
                     /translation="MPERVLIIGWDGVDWKVLRPMLDAGELPNLAALIERGAHGDCLS
                     SVPSHSWCAWPSFMTGLNPAGHGVFDILEHKPGVTKRLPVTYHSIKARTIFEDFSTAG
                     KTSLALNIPLTFPTPKVKGKVIAGGVLPAARSYTHPTELQKELDAKAPFPINGMSWTT
                     YRNRPEAFLEECAEITEKRQKAFEYLLDTTDWDFGVLVYVSTDRIQHCLMEYISPDHP
                     RYEELKDTPVAKQTRAVYQQLDDGLAKLLERTTERDLVIFMSDHGHQPCTRACTMDRV
                     LEHLGYAKFSKGSFVTNLIRWGPGRRIARRIYDLLKLHGRIALPATPLDWTKSRAYTS
                     VSSTGEGVSINLKGREPQGTVDPKDYESLREEIFQALSAFRDPDTGKAPIRKIYRKEE
                     VLSGRFLDTAPDLLLVPAPQYSLTHAKTMVEDADWLSGDHRLEGVVIATGPEVTPGPL
                     QETAELIDLGPTCMAALGVASSIPRDGKVLRSLVGEAQLQVSDAGSGAEGDAGDGGLT
                     SSEESEIEEHLRGLGYVE"
     gene            5807..7696
                     /locus_tag="VNP73_10355"
     CDS             5807..7696
                     /locus_tag="VNP73_10355"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HWL66363.1"
                     /translation="MGRRAIRIATLVAACCALLVATAPQAVAKEFLLPEATVTAHVMP
                     NGAVRVVEELTYEFYGPFSGAYREIPLREGETITNISVREGAVRYVPGAPTELGSSGS
                     PGSFGAASYDDDMRIVWHYSAFNERRMFTVSYTLNGLAVAYTDVVDVNLRVWGDEWAV
                     GLGSLRASIQMPRAAPEEVRVWGHPGDIQGETTLLQDGTGGTLAATNIPSGRWVEMRL
                     VFPRGVLLNTSKAEVRAEPGLQAILAEERAFARAADRLVWLADNAYWLIPLGFLLSFL
                     PALLVGFWVWRKYGREPDIPFMPGYVMEPPSDDPPALIPALLNPGYQRATGDAFAATL
                     FDLIRKKHIDAMTMTTIKKTWGGLREEDVSDLGLTVSDSNEGLEPYERTVKDAVAHAY
                     AGQKMLVLSFFDDVIKKEPAHYQKVFTDFKAEVRQAFTARGWWVGKGLKSLALAFAAA
                     VGFGILCIISLAIGGEAGRGPTVGSILAIAGIFMAAGNGVVLVVLMIARRGWEKRTPD
                     AALLAAKWNSFKKYIIDFPGIKEAAPGSIAIWEQYLVYGIALGVAERVLAAAQLHAPP
                     ELQTTSNVYFISSHGSLGSGHSAFAISDISHAVASASVSSSGGGGGFSGGGGGGGGGG
                     GGGAW"
     gene            complement(7753..8982)
                     /locus_tag="VNP73_10360"
     CDS             complement(7753..8982)
                     /locus_tag="VNP73_10360"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWL66364.1"
                     /translation="MSSTSHDRIVTGFVERLDEVADRCTKAIWDTVSTYAKSGDELRD
                     DVGTAVRDNVGTLAQVLKEQREIRKEELEAIERTGARRAEAGIPLDDVLHAYRTVSRV
                     CWDILADECRSYEGDALDAAIELAEAVLRYTDQISTSVAEAYAQAQRAIVRHQEGERR
                     EFLADLLYGSDAPPEDLLSRAHTFGYDLTLSYIALVGVGPKTDQGREVAVIQAAAATG
                     PLSTDPIVLERAGQTVALLPSDRFADVAIVPEKMVAELGGEWCFGVGGPELGLEGIRR
                     AYIEAKEALEIGLGLGLEGRIFRFDDLLLYHFLRTNPELVDRYVGQMLGPLIEYDERR
                     KGELIKTLESYFRADGSVKMAGELLFAHPHTVTYRIKQIEKLTGWSLRDAEDKLRLQL
                     ALRAYRLSQARRTEAAD"
     gene            complement(8979..9389)
                     /locus_tag="VNP73_10365"
     CDS             complement(8979..9389)
                     /locus_tag="VNP73_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWL66365.1"
                     /translation="MSEARADADYGFAVEVPVGYDEAIIRTRLALRAEGFSILSEMHV
                     GGIIVPEAGTERQYLIMGFWNTAVSEERIASDLRAAVHLPCNVVVQEQGEGSIVAALD
                     PADTVDEGATPPGLTDQARQALERVLKHVEEQES"
     gene            9410..10273
                     /locus_tag="VNP73_10370"
     CDS             9410..10273
                     /locus_tag="VNP73_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HWL66366.1"
                     /translation="MTLVAVPNVSEGRDAVRIAALARAMERGGARILDVHSDGRHDRS
                     VLTAAASPTDLVDACVRLAVEADRLIDLEGHTGVHPLLGKLDVCPFVVEPGSTEAIEA
                     ARATGAGIGELGIPVYLYGLASEPDRATELPALRKGGLAALIERARSGLTPDFGPPEI
                     DPLRGVVCVGARGPLIAFNVELATDVATARSIAARVRTSGGGPPGVRALGFDLGDRSQ
                     VSVNLIDPEVTGIETVFEAIASEAMAVDVEVIATEIVGLVARRFLPSRDAKATRLLVE
                     PGRSLESVLRR"
     gene            complement(10270..10659)
                     /locus_tag="VNP73_10375"
     CDS             complement(10270..10659)
                     /locus_tag="VNP73_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66367.1"
                     /translation="MEVLQLAAKKSTGRKRSTARKAPARKRSTARRKSTARKSPARRK
                     STARKKSTARKPARRKSTARKPARRKSTARKKSTARKPARRKSTARKPARRKSTARKK
                     STARKPARRKSTARKRTTRKRSTARKR"
     gene            complement(10901..11173)
                     /locus_tag="VNP73_10380"
     CDS             complement(10901..11173)
                     /locus_tag="VNP73_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005872211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWL66368.1"
                     /translation="MQRITTKLLQPVDWQANARCSEVDPEIFFPERGGSSKAARAVCD
                     RCAVRSQCLEYALNNKEQFGIWGGTSERERRKLRKDRALKRLRAVS"
     gene            complement(11325..11708)
                     /locus_tag="VNP73_10385"
     CDS             complement(11325..11708)
                     /locus_tag="VNP73_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWL66369.1"
                     /translation="MPDPTEPHSTLLVVSDDPHIREETRFGFSDEIGVVFADDSRTAM
                     EQMRSIVPELVLVDIQTGSAGGFSLARDMAQSPRLRAIPIFMLLERAQDGWLARQAGA
                     ALHRVKPIETEELVRSVLDLMSRVA"
     gene            complement(11714..12007)
                     /locus_tag="VNP73_10390"
     CDS             complement(11714..12007)
                     /locus_tag="VNP73_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66370.1"
                     /translation="MKIEATCDTCSRTFLLSQIGPDAEAPGRCPFCGARFARHYSTVL
                     AESVQGAENAATSFVHILARLQSMETGFDIDIEGLVGEISRQVRGNDSHARPA"
     gene            complement(12004..13356)
                     /locus_tag="VNP73_10395"
     CDS             complement(12004..13356)
                     /locus_tag="VNP73_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWL66371.1"
                     /translation="MPQTHRRLGAMDLAVLWGDLGVGLLVLVSGALLVPALGFAQALG
                     AIVLGSILGVGLLAFGAAAGATHGVPTMVLLRPLLGIRGSWIPSALNALQLVGWTAVE
                     FWAMSLVADLVSQRVFGFSARPLWLGIAAVICIGLALWGPVGVTRIWMERFAAWVVLG
                     IASILTLILIIEGEVAAAFSVPGQGGLGFGSAVDLVIAMPISWLPLVADYTRLARDPR
                     TAARGTFAGYLIANIWLYALGALLVLGPGAEPSPAGIAAGVLALTGGTIAGVLFLLAL
                     LVGETDEAFADIYSGAITVQNIWPDIPQKFLVAGIGIIGAVLAGWLTMDRYEAFLFLI
                     GSVFVPLFGVFLADHFVNRRNRIEVPDLYRAGGRYWYSGGFRLPIVVPWLVGFLVYHW
                     IVPVGPLWWVDAVSGVIDTPLSARVPWLSASIPAFLVSFGLTLVPGLSSTRPTKERTR
                     "
     gene            complement(13434..>13746)
                     /locus_tag="VNP73_10400"
     CDS             complement(13434..>13746)
                     /locus_tag="VNP73_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWL66372.1"
                     /translation="PKMLDIPVDQIDLEFANSEYSLLEEFSTDPFSKFIGLGVTDVHT
                     HAIESVDEIAEGIRKSLKFISPEKMFVDPDCGLKTRTVDEAKEKLVNIKKAVDVVRSE
                     L"
BASE COUNT         2476 a         4435 c         4237 g         2598 t
ORIGIN      
        1 tctttaccgg cctggcccat cgaccgcgcg agcttgggaa gtcgtgtcgc accgaagagc
       61 aggagaagga ccagcaggat gatgatgagt tcgccgccac cgggcaggtt catgacgttc
      121 ctccgttcag ttcccgtaac gccggagcga tctccgagcc gaagagttcg acgtcttcct
      181 cgtcggccag ctggaacgga atcgcaccga gcccggcgac cacttcttcg accccaagct
      241 ctgcgagtct cgacaagcga tcgacgacct cgtctaccgt tcctgccacg cggtcttgct
      301 tgaactcgtc ccaggatact ccggtggccc ctccgcccga ccgcaatatc cccggtggag
      361 tgcgctcgac gaggcgctcg aagcgcctgc gtacatcggc ctcactcgtg ccggccagca
      421 tgtagacacc gaccgatcga cgaagggtgc gtggatcgcg atccgcctct tcgcaggcct
      481 tgtccgccgc ggcagctcgc tcctcatagg tcgagaaagg tcccatccac gagaagttcc
      541 agccgtcggc tttacgcgct atcgcaccga gcgtccgatc gccttttccc ccgacccaga
      601 ttggcggcct cggcgatcga ggaccgagtg gccgaacgat cgctccgtcg atcgtgtagt
      661 ggtcgccttt gaatacgagc tcttctccgt cgaggagacg gccgatgatc tcgacggctt
      721 cctcgagcct ggcgatcctg gtgcgagcgt tgtgaaactc gatgcctgcg gcgcggtatt
      781 cgggttcgta ccagccggcc cccaggccca gatcgacccg gccgccggaa agctcgtcca
      841 gcgaagcgac catcttcgcg acgagggccg ggggccggaa gtcattgcat gcaaccatcg
      901 accccacccg aacgcgatcc gtgatcgcag ccagcgcgct cgtaagggtc caacactcga
      961 acgtcccttg gacggtgtcc gggcccccgt acttgctcca gtcgaggaag aaatggtcag
     1021 acacccacag cgaatcgaac ccgacccgtt ctgcagtacg agcgatcctc gatacgcctt
     1081 cccacgaaac gggttgcccg gccaggcttg tgtcgtagtg agggagcgca aggccgagct
     1141 tcatcgcggg agtttaggat gatcaggtga aagtctcaga catcatgact gcggcgtgcc
     1201 tatcggactc tcccgatgac accgtcgcgg aagcggccac caagatgcgg cagaaccaaa
     1261 cgggatcgat cctcgtgatg gattcgggga aactcgccgg gatcctgacc gagagagacg
     1321 tcctgcgagt cgtcgccgag ggcaaggacc ccaagtctgc ggtggtcaag gacgagatga
     1381 cggacaaccc cgtcacgatc ccgacccaca cgagcctcaa agacgccgct caaacgatgt
     1441 tcgacaagtg gttccggcac ttgccggtgg tgacgaacga gggcgatgtc gtggggatca
     1501 tctcgctgcg cgacctacta acgatcgttg cagagagcct ggatgagccc gaggcgatcc
     1561 aatcgctgac cgggcacaag ctggtgcgcg accgccgtct cgagcgtgtc gaagccggcg
     1621 acctcgactg atcccattcc gggaccgagg agccctcatt tcatccgggc ctggtctttc
     1681 ggacttctct cgatcgcgag tccgttcctc gttccttttg tcgctcggct gatccaaccc
     1741 ggcgcagagg tcggccggat cgcttatcta tccgccttgg gggccgcagc cgcggtttcc
     1801 ttgctcggcg taatcttcgc cgttctcacc cagaggcaga ccgcgcccaa gacccttcaa
     1861 cgcaggatcg ccgggatcac gggcgcgctc agtggcgtga tgttgctgtt ctggttcgtg
     1921 agcacgctgg ccctgctgaa gctcagcccc tcctggcgat agcgccgttc ttgtcgcgtc
     1981 agagcgcgct ggaaggttgc tcagttagcc aggaacggtt aggtgagctt gtagcgctcc
     2041 aggaggcggc ggccgatcac gttcttctgg atctcggacg tcccttcgcc gatcagcagg
     2101 aacggcgcat cgcggtagag acgctcaaca gtgaactcct tcgagtagcc gtagcccccg
     2161 tgaaccctca gcgacttcgt cactatctcg tggcacgcct cggaggcgaa cagcttcgcc
     2221 atcccggcct cgagatccga gcgctcgccg gccaccttct tctgggccgc cgagtacagc
     2281 atcagcttcg ccgcttcgag cgtcgtgccc atctcggcga tgtggtgcag gacggcctgg
     2341 tggtctgcga tctgtttgcc gaacgcctta cgctcctgcg cgtacttgat cgcctcttcg
     2401 aaagctcgcg tcgagacgcc gaccgctcgc gccgccacgt tcacgcggcc cacctcgatc
     2461 ccatcgagca cctgcttgaa tcccatgcct tcctcggtgc ccagtagctg cgaggtcgag
     2521 cagcggtagt cggagaacgt catctcgacg gtctcgacgc ccttgtagcc catcttctcg
     2581 atgttcttgc caacgtcgag acccgccgcc ggcttgggct cgccgggcgt cttctcgaca
     2641 atcaacatgc tcatgcccga gtggggtggg tcggcgttcg ggtcggtctt caccaaggtc
     2701 atcacgatgc cggcgcgcag tccgttcgtc agccacatct tctggccatt tacgacgtac
     2761 tcgtcgccct ccttgatcgc tttagtgcgg atcgcttgca catcggagcc ggcctcgggc
     2821 tcggacatcg cgagagcggc ccggatctcg ccggtagcca tcctcggcag atagttcttc
     2881 ttctgctcgt cggtgccgaa cttgttgatc aagtaacagc ccatgaagtg cgtgttgagg
     2941 acacccgaga ggctcatcca gccacgggcg agctcgacga cgcagagggc ataggtgatc
     3001 agcgactcgc cgatcccgtc gtactcctcg ggaacggtga tgccgaacag ccccatctcc
     3061 ttcatctgtt cgacgatctg ctcgggatac tcgtccttgt gctcgaggtc gtcggctatc
     3121 gggatgactt ccttctcgac gaagtcgcga accgcttcga ctagtgcctt ctgggtgtcg
     3181 ttcagttcga tcatcagctc ttctccgccc attccttctc gaagtcggcc aatgtcttgt
     3241 tgggcttcag accggcagcg cggccccgct cggcgaactg cacggccatc ttgcgggaag
     3301 cctcgtcgat catctcgttg ccaaacatca cggcgcccgt gcgatcgacc tcagtcgcat
     3361 gcttgtaagc gtcgaggagc gcctgggcct tgtcgaactc ctcctgtgtc ggagtgaaga
     3421 cctcgttggc gacccctatc tgacccggat gcagcgccca cttgccgtcg tatccgagag
     3481 cccgcgcccg catcgccatg tcgcgatacc cgtcgacgtc cttgatcaac agatacgggc
     3541 cgtcgatcgc ctgcaagccg gcatcgcgtg cggctacgag gatcgtttcg agaacccaat
     3601 ggaagtggtc gcccgggtaa cccggcatcg gcagtccggc cgtgacggtc gggagaccaa
     3661 gtgatgcgct catgtcggcg gggccgaaga tcaacgtctc catccggtcc gacgcatgag
     3721 cgatctcgtg gatgttcatc aatccggtcg cggtctcgat ctgaacctcg aggccgatcc
     3781 gcttctcgag tcccacggtc gtctcgatca tccggagcat gtgatcgacg aacatgacct
     3841 cgtgcgcgta ctggaccttg gggatcatga cgcagtccag gtactggccg gcgttctcga
     3901 cgatcgtctt gaggtcgtcc gcggcccact gcgtatcgat cgagttgata cggacgacga
     3961 cggtcttgtc ggcccagtcg ttgttcttga gagcgtcgat cacattgccc cgcgcttcct
     4021 ctttagcgag gggcgcgacc gaatcctcga ggtccatgaa gacctcgtcg gccggaagag
     4081 ttgggccctt cgacagcatc ttggggctcg aaccgggtac agagagacag gatctgcgtg
     4141 ctcgtgctac cgccacggga acttcctcct acttgatcga cgcgtctatt cgacgtaacc
     4201 gaggcctctt aggtgctcct cgatctcact ttcttcgcta gaggttagtc cgccgtcgcc
     4261 ggcgtcgccc tccgcgccgg acccagcatc ggagacctgt agttgtgcct ccccgaccaa
     4321 ggagcgcagc accttgccgt cccgaggtat cgaagacgca acgcccaggg cggccatgca
     4381 ggtcgggccc aagtcgatga gctcggcggt ttcttgcagg gggccgggag tgacttcggg
     4441 gccggttgcg atcacgacgc cctcgaggcg atggtcgccg gacaaccagt cggcgtcctc
     4501 gaccatcgtc ttcgcgtggg tcaacgagta ctgaggggcg gggacgagga gaagatccgg
     4561 cgccgtatcc aggaaccttc ccgagaggac ctcttccttg cgatagatct tgcggatcgg
     4621 agccttaccc gtgtcgggat cgcggaacgc gctgagggcc tggaagatct cctcgcgcaa
     4681 cgactcgtag tccttcgggt cgaccgttcc ttgaggttcg cggcccttga ggttgatcga
     4741 tacgccttcg ccggtcgaac tcacgctcgt gtacgcgcgc gacttcgtcc agtcgagcgg
     4801 cgtcgccggc aaggcgatgc gaccgtggag cttcagcagg tcgtagatcc tgcgggcgat
     4861 ccggcggccc ggcccccagc ggatcagatt cgttacgaag gagcccttcg agaacttggc
     4921 gtaacccaga tgctcgagaa cgcgatccat cgtgcaggca cgcgtgcacg gctgatgacc
     4981 gtggtcagac atgaagatca cgagatcgcg ttcggtcgta cgttcgagca acttagccag
     5041 tccgtcgtcg agctgctgat agacggcgcg cgtctgcttc gcaaccgggg tgtctttcaa
     5101 ctcctcgtag cgtgggtggt ccggcgagat gtattccatc aagcagtgct ggatgcgatc
     5161 ggtcgagacg taaacgagaa caccgaagtc ccaatcggtc gtatccaaga ggtactcgaa
     5221 agccttctga cgtttctcgg tgatctcggc acactcttcc aggaacgcct ccggccggtt
     5281 cctgtacgtc gtccaggaca tcccgttgat cgggaatggt gccttcgcgt cgagttcctt
     5341 ctgcaactcg gtggggtggg tgtaggagcg cgcggccggg agcacacctc cggcgatcac
     5401 cttgcccttg accttcgggg ttgggaacgt cagtgggatg ttcagcgcga gcgaggtctt
     5461 accggccgtc gagaaatcct cgaagatcgt tcgagccttg atcgagtgat acgtgaccgg
     5521 caggcgcttg gtcacgccgg gcttgtgctc gaggatgtcg aagaccccat ggccggccgg
     5581 gttcagtccg gtcatgaatg agggccaggc gcaccatgaa tgagagggaa cgctagatag
     5641 acagtccccg tgcgctcccc gttcgataag ggcggcgaga ttcggcaatt cccccgcatc
     5701 gagcatcgga cgaagtacct tccaatcgac gccgtcccag ccgatgatga gcacgcgttc
     5761 gggcaacaga ttctccttgt caggcggtcg taagagggta gcagcgatgg gtagacgagc
     5821 catacggata gcaacacttg tagccgcttg ctgcgcgctc ttggtcgcca ccgcaccgca
     5881 agcggtcgca aaggagttcc tgctgcccga ggcgacggta accgcccacg tgatgccgaa
     5941 cggagccgtg cgtgtcgtcg aggaactcac ctacgagttc tacgggccct tctcgggcgc
     6001 ctatcgggag atcccactgc gtgagggcga gacgatcacg aacatctcgg tgcgagaagg
     6061 ggccgtgcgc tacgtccccg gagcgccgac ggaactgggg agctcgggaa gccccggatc
     6121 gttcggtgcc gccagctacg acgacgacat gcggatcgtg tggcactact ccgcgttcaa
     6181 cgagcgtcgg atgttcaccg tttcgtacac gttgaatggc cttgcggtcg cgtacacgga
     6241 cgtcgtcgac gtgaacctgc gcgtgtgggg cgacgagtgg gccgtcgggc tcggttcgtt
     6301 gagagcatcg atccagatgc cccgagcggc ccccgaagaa gtgcgcgtgt ggggacaccc
     6361 cggcgacatc cagggagaaa cgaccctgtt gcaagacggg acgggaggga cgctcgcggc
     6421 tacgaacatc ccgtcggggc gatgggtcga gatgagactc gtgtttcctc gcggcgttct
     6481 gctgaataca tctaaagccg aggtccgtgc cgagccgggt ctgcaggcga tccttgccga
     6541 ggaacgcgcc ttcgcacgcg ctgccgaccg cctcgtgtgg ctggccgaca acgcctactg
     6601 gcttatccca ctcggtttct tgctgtcgtt cctgcccgcc ttgctggtcg gcttttgggt
     6661 gtggaggaag tacggccgcg agcccgacat cccgttcatg ccgggctacg tcatggaacc
     6721 tccgtcggac gatcctccag ctctaatccc ggctctactg aaccccggct atcagcgcgc
     6781 aaccggcgac gccttcgccg cgacgctatt cgacctgatc cgcaagaaac acatcgacgc
     6841 gatgactatg accacgatca agaagacgtg gggcggactg cgagaggaag acgtgagcga
     6901 tctcggtctc acggtctcgg attcgaacga gggcctcgag ccgtacgagc gaaccgtgaa
     6961 ggacgcggtg gcccatgcct atgccggtca gaagatgctg gtcctcagct tcttcgacga
     7021 cgtcatcaaa aaggaacccg cgcactatca gaaggtcttc accgacttca aagccgaggt
     7081 gagacaggcc ttcaccgcga ggggctggtg ggtcggcaag ggcttgaaat ccctggcgct
     7141 ggcgttcgcg gccgcggtcg gcttcggaat cctgtgcatc atcagtttgg ccatcggtgg
     7201 cgaagcggga agggggccca ccgttggttc gatccttgcg atcgccggca tcttcatggc
     7261 agccggcaat ggggtcgtgc tggtcgtcct gatgatcgcc cgccggggat gggagaagag
     7321 aacccccgac gccgcgcttc tcgcagccaa gtggaactcc ttcaagaagt acatcatcga
     7381 cttcccgggg atcaaggaag ccgcgcccgg ctcgatcgcg atctgggagc agtatctggt
     7441 ctacgggatc gccctggggg tggcagaacg cgtgttggcg gcggctcaac tgcacgcccc
     7501 acccgagctg caaacgacga gcaacgtgta cttcatctcc tcgcacggga gcctgggctc
     7561 cggtcactcg gcgttcgcca tctccgacat ctcgcacgcg gtcgccagcg cgtcggtctc
     7621 gtcgtccggc gggggtgggg gcttctccgg cggtgggggt ggcggtggag gaggcggtgg
     7681 tggcggtgcc tggtaaccct tttcttctaa ctcacggccc atatagggca cctcagttag
     7741 aacagaaggg gtttagtcgg cggcctcggt ccggcgcgcc tgcgacaaac ggtaggcccg
     7801 cagagccagc tgtagccgca gcttgtcctc ggcgtcccgg agtgaccagc cggtcagctt
     7861 ctcgatctgc ttgatccggt aggtgaccgt gtgggggtgc gcgaacagaa gctcgccggc
     7921 catcttcacg ctgccgtccg cccggaagta ggactccagg gtcttgatca gctcgccctt
     7981 ccggcgctcg tcgtactcga tcagggggcc gagcatctgc ccgacgtagc gatcgaccaa
     8041 ctctgggttc gtccggagga agtggtagag gagaaggtcg tcgaagcgga agatgcgccc
     8101 ctcgagccca aggcccagtc cgatctcgag cgcctctttg gcctcgatgt acgcgcgccg
     8161 gatgccttcc aacccgagct ccggcccccc caccccgaaa caccactcgc cccccagctc
     8221 ggcgaccatc ttctcgggaa cgatcgcgac gtcggcgaat cgatccgagg gtagaagagc
     8281 cacggtctga ccggctctct cgagaacgat ggggtcggtc gaaagggggc cggttgcggc
     8341 cgccgcctgg atcaccgcga cctcgcgtcc ttgatcggtt ttggggccca cacccacgag
     8401 ggcgatgtaa ctgagagtca ggtcgtatcc gaacgtgtgc gcccggctga gtagatcctc
     8461 cggcggcgcg tcggatccgt acaacagatc ggcgaggaac tcgcgacgtt cgccctcttg
     8521 gtgtcgaacg atcgctcgct gtgcctgtgc atacgcttcc gcaacactcg tagagatctg
     8581 gtcggtgtaa cggaggaccg cctctgcaag ttcgatagcg gcgtcgagcg cgtcaccttc
     8641 gtaactcctg cattcgtccg ccaggatgtc ccagcaaacc ctcgagacgg ttctgtacgc
     8701 atgcaacacg tcgtcgagcg gaatccccgc ctctgctcgc cgggcccccg ttctctcgat
     8761 cgcttcgagt tcttccttgc ggatctcgcg ctgctctttc agcacctgag ccagcgtgcc
     8821 cacgttgtcg cgcacggccg tacccacatc gtctcgcagc tcgtcaccgc tcttggcgta
     8881 agtcgagacg gtgtcccaga tcgccttggt gcaccgatcg gccacctcgt cgagccgttc
     8941 gacgaacccg gtgacgatgc ggtcgtgaga ggtcgagctc atgactcttg ctcctcgaca
     9001 tgctttagca ctcgctccag tgcttggcgc gcctggtccg tgaggccggg cggagtcgcg
     9061 ccctcatcga cggtgtcggc ggggtcgaga gccgccacga tcgaaccttc tccctgctcc
     9121 tgaacgacca cgttgcaggg aaggtgaacg gccgcgcgga gatccgaggc gatcctctcc
     9181 tcactcacag cggtattcca gaaacccatg atcagatact gacgctcggt tcccgcttcg
     9241 gggacgatga ttccgccgac atgcatctca ctgaggatcg agaaaccctc ggccctcagg
     9301 gcgagtcgag tacggatgat cgcctcgtcg taaccaaccg ggacttctac ggcgaatccg
     9361 taatctgcgt ctgctcgagc ttctgacaca tccactacgg taacgcgaca tgacgctcgt
     9421 cgctgttccg aacgtttctg aaggccgaga cgccgtgcgg atcgccgccc tggctcgcgc
     9481 gatggaacga ggcggcgctc ggatcctgga cgttcattcc gacgggcgac atgaccgaag
     9541 cgttcttacc gcggcggcat cgcccacgga tctcgtggat gcctgcgttc gattggccgt
     9601 cgaggcggac cgcctcatcg acctggaggg ccacaccggc gttcacccgc tcctcgggaa
     9661 gctggatgtg tgcccgttcg tcgtcgagcc cggttcgacc gaggccatcg aggcggcccg
     9721 cgcgaccggc gcggggatcg gcgagctggg cataccggtc tatctctacg gcctggcctc
     9781 agagccggat cgggcaacag agctgccggc cttgaggaag ggggggctcg cggcattgat
     9841 cgagcgagcg cggtccggcc tgacgccaga cttcggcccc cccgagatcg atccccttcg
     9901 tggtgtcgta tgcgtcggcg cccggggccc cctgatcgcc ttcaacgtcg agctcgcaac
     9961 cgatgtggca acggcgcgtt cgatcgcggc tcgtgttcga acgagtggtg ggggcccgcc
    10021 gggtgtgagg gccctcggat tcgatctggg agatcggtct caggtctcgg tcaatctgat
    10081 cgaccccgag gtcacgggga tcgaaaccgt cttcgaggcg atcgcatccg aggcaatggc
    10141 ggtggacgtg gaggtcatcg cgacagagat cgtcggcctt gtggcccggc gcttcctacc
    10201 gtctcgagac gcgaaagcga cccggctcct cgtcgaaccg ggtcgctctc tcgaatctgt
    10261 gttacgacgc tagcgcttgc gagcggtcga acgcttgcgc gtggtccgct tgcgcgccgt
    10321 gctcttcctg cgcgccggct tacgagcggt gctcttctta cgagcggtgc tcttcctgcg
    10381 cgccggctta cgagcggtgc tcttccgacg agccggctta cgagccgtgc tcttcttgcg
    10441 agcggtgctc ttcctgcgag ccggcttacg agcggtgctc ttccgacgag ccggcttacg
    10501 agccgtgctc ttcttgcgag cggtgctctt cctgcgagcc gggctcttgc gcgctgtgct
    10561 cttccgacga gcggtcgagc gcttccgcgc cggagcctta cgagcggtcg aacgcttccg
    10621 accggtgctc ttcttcgctg ccaattgcag cacctccatt ccccacttcg acgcgatccc
    10681 caactgcgat cacgacattc ctccccgact acacgatgct gatgtgcagt cagctcagca
    10741 atgaatacgc gcacaaacgc gcattcacgc agtaattagg cactatttgc gcgcttggtg
    10801 caagcattta aataaccaac gcgttattcg tgtcaagtgt ttccgacgcg tttgcacgtg
    10861 acgcgcgcat gaaaatcact taagaagagt gatctaagtg ttatgagacg gcgcgcaaac
    10921 gcttgagcgc gcggtccttg cgcagtttgc gacgctcgcg ttcagacgtt ccgccccaga
    10981 taccgaactg ttctttgttg ttgagcgcgt actcgagaca ctgcgaacgc acggcgcacc
    11041 tgtcgcacac ggcgcgcgcc gccttagacg agccgccgcg ttcgggaaag aagatctcgg
    11101 gatcgacctc cgaacacctg gcattcgctt gccaatcgac gggctgaagg agcttggtag
    11161 ttatccgctg catttccccc ggcctttcct ctttgcttcc ccgttgccat ccgggaccgc
    11221 tttcaccacc cgaatgacat tgttgtaatt ctaatgttgt aattcggtat gccgtcaagt
    11281 ggcacttttc gcgctcgggg ggcggttcgg gaggttttgt tctcttacgc gacgcggctc
    11341 atgagatcga ggaccgatct gacgagctct tcggtctcga tcggcttaac tcgatgcagt
    11401 gccgcgccgg cttggcgagc gagccatcca tcctgagcac gctccaacaa catgaagatc
    11461 gggatggctc gcaatcgagg cgactgggcc atatccctcg ctagtgagaa cccaccggcg
    11521 ctgccggtct ggatatcaac cagtacaagc tcaggaacga tcgaccgcat ctgttccatc
    11581 gcggtacggg aatcgtccgc gaaaaccacg ccgatctcgt cggagaaccc gaaacgggtc
    11641 tcctcccgga tatgggggtc gtccgagacg accaacaggg tggaatgcgg ctccgtcggg
    11701 tcgggcacga aggtcaggcg ggacgagcgt gggagtcgtt tcctcggacc tgcctcgaga
    11761 tctcgccgac gagcccctcg atatcgatgt cgaacccggt ctccatggac tggagccggg
    11821 cgagaatgtg cacgaaactg gtagcagcgt tctccgctcc ctgaacagac tcggcgagga
    11881 ccgtcgagta gtgccgggcg aagcgcgccc cgcagaaggg gcaccggccc ggcgcttccg
    11941 cgtcgggacc gatctgcgat agcaggaacg tcctcgagca cgtatcgcac gtcgcttcga
    12001 tcttcatcgt gtcctctcct ttgtgggccg ggtcgatgat aggccgggta ccagcgtgag
    12061 cccgaacgac accaagaatg cgggtatcga agcactgagc cacggcacgc gggccgaaag
    12121 aggtgtgtcg atcaccccgg atacagcgtc cacccaccac agcgggccca ccggaacgat
    12181 ccagtgatag acgaggaatc cgacgagcca gggaacgacg atcggcaacc ggaatccacc
    12241 cgagtaccag taacggccgc cggcccgata cagatccggc acctcgatcc gattccttct
    12301 gttgacgaag tgatccgcga ggaacacccc gaacagaggg acgaagaccg agccgatcag
    12361 gaacaggaag gcttcgtaac gatccatcgt cagccatccc gccaacacgg cgccgatgat
    12421 gccgatcccc gcgacgagga acttctgagg gatatcgggc cagatgttct ggaccgtgat
    12481 cgcacccgaa tagatgtctg cgaatgcctc gtcggtctcc cccaccaata aggccagaag
    12541 gaagagaacg ccggcgatcg tccctcccgt gagcgcaagg acgcccgccg cgatccccgc
    12601 aggcgacggc tcggcccccg ggcccaacac aagcagtgct cccagggcat agagccagat
    12661 gttcgcgatc agataaccgg cgaaggtccc gcgtgcggca gtgcgcgggt cgcgcgccag
    12721 tcgcgtgtag tccgccacca gcggcaacca cgagatcggc atcgcgatca ccaggtcgac
    12781 ggccgaaccg aaaccgaggc cgccctgccc gggcacggag aacgccgccg ctacctcacc
    12841 ctcgatgatc aagatcaacg tgagtatcga tgcgatcccc aagaccaccc acgccgcgaa
    12901 ccgctccatc cagatgcgcg tgacaccgac cggcccccac aacgcgagac cgatacagat
    12961 gactgctgcg atccccaacc acagcggtcg tgccgagaac ccgaacacgc gctgagaaac
    13021 gaggtccgcg acgagcgaca tcgcccagaa ctcaaccgcc gtccatccca cgagctgcaa
    13081 cgcgttgagg gcactcggta tccacgatcc cctgatcccg aggagcggcc gaagcagcac
    13141 catcgtcggc acaccgtggg tcgcgccggc cgccgcgccg aacgccagca aaccgactcc
    13201 caggatggag ccgaggacga tcgctcccag agcctgggcg aagccgaggg ccggaacgag
    13261 gagggctccg gacaccagga cgaggaggcc cacacccagg tccccccaca ggacggcgag
    13321 atccatcgcg cccagccttc gatgggtctg cggcacgggg tcgacgcccc attcatggag
    13381 cgggtcggca ccctctaaga gggaactcaa tatgtgttcg cttcgatcgc cgcttagagc
    13441 tcggaacgaa cgacgtcgac cgctttcttg atgttgacga gcttctcctt ggcctcgtcg
    13501 accgtccgcg tcttcaagcc acagtccgga tctacgaaca tcttctcggg agagatgaat
    13561 ttcagcgact tgcggatccc ctcggctatc tcgtcgaccg actcgatcgc atgcgtatgg
    13621 acgtcggtga cccccaaccc gatgaacttc gagaagggat ccgtcgagaa ctcctcgagc
    13681 agcgaatact cggagttggc gaactcgaga tcgatctggt caaccgggat gtcgagcatc
    13741 ttcgga
//
LOCUS       DATMED010000397         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_695711, whole genome shotgun sequence.
ACCESSION   DATMED010000397 DATMED010000000
VERSION     DATMED010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695711"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VNP73_10405"
     CDS             <1..1357
                     /locus_tag="VNP73_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HWL66373.1"
                     /translation="SIVVVPSLSFPVEELRKIVGIEHYEERMLFMSLLLDRPDLEMVY
                     VTSAPIDEQVIDYYLSFLPDPIGARKRLHLVALDDTRPQSLSIKLLERRDKIDEIRSQ
                     LQKEPYALTFNVTDAEWQLMGALDVIVYGAHPELARLGSKSGARDVAGRAGVAVLPGA
                     SDLMSVDDVERAVVDLARDTSSSSAVIKLNNGFSGQGNAIVSLEGLRSPLVQTETVFC
                     ASEESWPSFEKKIAAEGAVVEQLMRDPGTVSPSTQMRIAADGSFEVVSTHDQILGGPD
                     DQVYLGCRFPAEDSYRDAIQEAGRRIATVLAEEGVVGSFGIDFVIASGDPTKTYLSEI
                     NLRMGGTSHPFYMARFVTRGHYDEATGNLMAGGRPKFYVASDNIKSGSYIGLRPEQII
                     EALDERGLAYDQGTRTGATLHLLGALQNYGKLGCVAIADSRDEAATLYDEVVGVLDSI
                     R"
     gene            complement(1358..2164)
                     /locus_tag="VNP73_10410"
     CDS             complement(1358..2164)
                     /locus_tag="VNP73_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HWL66374.1"
                     /translation="MRSARGRMLAAALAIHLVLLPSGASAHRFQRVLSFHSKSGDVRA
                     LEMRIAGWYPAHTQKLLRVDRYFGRRTTIAVKAFQRRFGLAVDGVAGPQTFSVLNRLE
                     SKDGSTQHFSWAEFWQHKNRSCSRRANRYAGTFKGGEVSSRAVRRHVKQLMWRLEAVR
                     SKARMPVIVESGFRSVPYNRCIGGATASQHLFGYAADVRIPGVSGEHEREIYKRSQIH
                     GVLCYSSSRHTHIDLRLDNRALPYLHQWWWPDRDGRGNHLTPEGSRCYGE"
BASE COUNT          454 a          722 c          771 g          456 t
ORIGIN      
        1 gtccatcgtt gtcgtgccgt ctctttcctt cccggtggag gagctccgca agatcgtggg
       61 tatcgagcac tacgaggagc ggatgctgtt catgtcattg ctgctcgacc ggcccgacct
      121 cgagatggtc tacgtcacct cggcccccat cgacgagcag gtgatcgact actacctgtc
      181 cttccttccg gatccgatcg gcgctcgcaa gaggctgcat ctggtggcgc tcgatgacac
      241 gcgcccccag tcgctgtcga tcaagctcct cgaacggcgc gacaagatcg acgagatcag
      301 atcgcagctc cagaaggagc cttatgcctt gacgttcaac gtgaccgacg ccgagtggca
      361 gctgatgggc gcgctcgacg tgatcgtcta tggggctcac ccggagctcg ctcgactcgg
      421 ctccaagagc ggtgctcgcg atgttgctgg gcgggcgggg gtggccgtcc tcccgggcgc
      481 ttcggacttg atgtctgtcg atgacgtcga acgggcggtc gtcgaccttg ccagggatac
      541 gtcttcgtcg tccgccgtca tcaaattgaa caacgggttc tcggggcaag ggaacgcgat
      601 cgtctctctc gaaggattga gatcccctct ggtgcagacc gagaccgtgt tctgtgcgtc
      661 cgaggagtcg tggccttctt tcgagaagaa gatcgcggcc gagggcgccg tcgtcgagca
      721 actgatgcgc gacccgggga cggtttctcc cagcacccag atgcggatcg cggccgacgg
      781 ttctttcgag gtcgtttcga cgcacgatca gatcctgggg gggccggacg atcaggtcta
      841 tctcggttgc cgcttccccg ccgaggattc ctatcgggac gcgattcagg aggccggtcg
      901 tcgcatcgcg accgttctgg ccgaagaagg agtcgtcggt tccttcggga tcgatttcgt
      961 gatcgcatcc ggtgacccga caaagacata tctcagtgag atcaacctgc ggatgggagg
     1021 aacgtcacat ccgttctaca tggctcgctt cgtaacgaga gggcactacg acgaggcgac
     1081 cggaaacctc atggccggag ggcgccccaa gttctacgtg gccagcgaca acatcaagtc
     1141 cgggtcctac atcgggctcc gtcccgagca gatcatcgag gcgctggacg agagaggctt
     1201 ggcctacgac caggggacga gaaccggcgc gacgctgcac ttgttgggag cgctgcagaa
     1261 ctacgggaaa ctcggttgcg ttgcgatcgc cgactcgcgc gatgaagcag cgactctcta
     1321 cgatgaagtt gtcggggtgc tggattcgat ccggtaacta ctcgccgtaa cagcggcttc
     1381 cctcgggggt gaggtggttg ccccttccat cgcgatccgg ccaccaccac tgatggagat
     1441 aggggagcgc gcgattgtcg aggcgcaagt cgatgtgcgt atgcctcgac gacgaatagc
     1501 acaacacgcc gtggatctga cttctcttgt agatctcgcg ctcgtgctcg ccggacactc
     1561 ctgggatgcg cacgtcggcg gcatacccga aaagatgctg tgacgcggtc gctccgccga
     1621 tgcatcggtt gtatggaacg ctgcgaaatc cggactcgac gatcaccggc atccgcgcct
     1681 tggagcgtac ggcctcgaga cgccacatca gctgcttcac gtggcgtcgc acagcgcggc
     1741 tggacacctc ccctcccttg aacgtgcccg cgtaacggtt cgctcggcgg gagcaggaac
     1801 ggttcttgtg ttgccagaat tctgcccacg agaagtgttg cgtcgagccg tccttgctct
     1861 ccaggcgatt caggaccgag aacgtctgtg gaccggccac gccatctacg gcgagtccga
     1921 agcgacgttg gaaggccttc accgcgatgg tggtcctgcg gccgaagtag cgatcgaccc
     1981 gcaacagctt ctgggtgtga gccggatacc acccggcgat ccgcatctcc agagcgcgga
     2041 cgtcaccgga cttgctgtgg aagctaagga cgcgctggaa ccggtgcgcg gacgcacccg
     2101 agggaagaag gacgagatgg atcgccagcg ccgcggcgag catccgccca cgggcagacc
     2161 tcattggtgc cccctagctc aaacccggcc aaagaccaca ataattaaca gtagtgtcaa
     2221 gaagtggatg ggtaactatc ccttgacgac agggaaccag ggcaaaacgg acatgggggg
     2281 ttccctttga cccggttcta ggctcgggac gtgggcaaga tttgggaccg ctatcgggaa
     2341 agccggcgaa caaccatgga gacgggccag gtccggctgt tctttcggat gctggccggg
     2401 atc
//
LOCUS       DATMED010000398         3764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5306432, whole genome shotgun sequence.
ACCESSION   DATMED010000398 DATMED010000000
VERSION     DATMED010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5306432"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VNP73_10415"
     CDS             complement(<1..246)
                     /locus_tag="VNP73_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HWL66375.1"
                     /translation="MTETTTSLQAPHVLEYPYTRSVGPVIGRFLEGLRDGRIEGIRTS
                     AGKVIVPPTGYDPETGADLTEFVEVGTSGVVTTWSWQP"
     gene            complement(251..1801)
                     /locus_tag="VNP73_10420"
     CDS             complement(251..1801)
                     /locus_tag="VNP73_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWL66376.1"
                     /translation="MAELGFWEVAQRHPENLALVDPDGTEMTAGELFAESNKLVHGLR
                     ALGVKQGDAVAAVLPNSTPLIELYLACMQSGLYLVPINHHLAAPEIAYILEDSEAKAL
                     FTSEEFGEVSTKAVDDASLPKEARFVDGKLDGFRPYAEIKEGQPGTLPEDRATGAVMN
                     YTAGTTGRPKGVRRAVPQIDPDTAAELYTLFFVLFGIEPYNDNVHVVVSPLYHTAVMT
                     FSMTSLHAGHAVVLMKKWDEEETLRLIDRYKVTNTHMVPTQFRRLLDLPREVKDKYDV
                     SSLRQVVHSAAPCPVDVKERMLDWWGPVIYEYYAATEGGGTLATPEEWLKKPGTVGKP
                     WPMSEIRVLDDDGQPMPTGEAGTVWIKMGDYTFEYNKDKAKTEESWKEGFFTVGDVGY
                     LDEDDYLFLCDRKADMIISGGVNIYPAEVEAELVGHPKVDDVAVIGIPNDQWGEEVKA
                     VVKPTPGNEPGPALEQELIEFCRERIAHFKCPRSVDFVTELPRDPNGKLYKRKVRDPY
                     WQGRTSAI"
     gene            1902..2981
                     /locus_tag="VNP73_10425"
     CDS             1902..2981
                     /locus_tag="VNP73_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWL66377.1"
                     /translation="MRAALLRELNAELEVRDDFEVKGPGPGEVRVRIAASGVCHSDLS
                     LQNGTLPHPIPAVPGHEGAGEIIAVGEGVRGLAEGDHVIITWVPPCGDCPSCVEGQPY
                     LCGAFLEPSLTDPHFTSDGLAAFGFAGTGTFAEETVVPQEAAIKIDKDIPFDIAALIG
                     CGVMTGVGASLNTAQIRPGSNVVVFGCGGVGISVIQGARIAGAAEIVAVDVMEPKLDL
                     ARQFGATHACLPNDLPAISSEVTKDRGFDYALEVVGQPETIRAAWDATRRGGETIVVG
                     AGAMDAFVSFSAFELFYSAKTLKACWYGSGNVRTDFHKFLRFWDQGKLDLEGMITRRV
                     DLSEINEAFTACAKGELVRALVEFK"
     gene            3069..3602
                     /locus_tag="VNP73_10430"
     CDS             3069..3602
                     /locus_tag="VNP73_10430"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HWL66378.1"
                     /translation="MRALHIIGGPPGAGKTSSAGLVVDTYQRGVHIRSDDFWHYIASA
                     YVEPWLPASADQNAVVMSAVASAAARFVIGNYDTIVDGAIGPWFLDPFIEVAKGSGFV
                     LDYVILRPDREVALARALKRVDGGLKERGPVLKMHEEFSSLAEFERYVLDSSAMSPEG
                     TAAALRSGLEEGRFRVI"
BASE COUNT          672 a         1113 c         1211 g          768 t
ORIGIN      
        1 gggctgccac gaccacgtcg tgaccacgcc ggatgtgccc acctcgacga actcggtgag
       61 atcggccccc gtctccgggt catatccggt cggaggaacg atcaccttcc ccgcggaggt
      121 gcgaatgcct tcgatccttc cgtcccgcag cccctcgagg aaacggccga tcaccggacc
      181 gacgctgcgt gtgtaggggt attcgaggac gtgcggcgct tgcagagaag tcgtggtttc
      241 ggtcatcgat tcagatcgcg ctcgtgcgtc cttgccagta cgggtcgcgc accttgcgct
      301 tgtagagctt gccgttcggg tcacgaggta gctcggtcac gaaatccacc gagcgcgggc
      361 acttgaagtg cgcgatgcgt tcgcggcaga actcgatcaa ttcctgttca agagcgggac
      421 ccggttcgtt ccccggggtc ggcttaacga ccgccttgac ctcttcgccc cattgatcgt
      481 tcgggatgcc gatcactgcg acatcgtcga ccttgggatg accgacgagc tcggcctcga
      541 cctcggccgg atagatgttg accccgcccg agatgatcat gtcggcctta cggtcgcaga
      601 ggaaaaggta gtcgtcctcg tcgaggtagc cgacgtcgcc tacggtgaag aatccttcct
      661 tccacgattc ttcggtcttg gccttgtcct tgttgtactc gaaggtgtag tcgcccatct
      721 tgatccacac ggttcccgct tcaccggtgg gcatcggctg tccgtcgtcg tcgagcacgc
      781 ggatctcgga catcggccac ggcttgccga ccgtcccagg tttcttcagc cactcttctg
      841 gagtagcgag ggtgccaccg ccttcggttg ccgcgtagta ctcatagatg accggccccc
      901 accaatcgag catccgttcc ttgacgtcga ccgggcaggg ggcggcgctg tgaacgacct
      961 gtcgaagaga cgagacgtcg tacttgtcct tcacctcgcg cgggaggtcg agcaaacggc
     1021 ggaactgcgt cggtaccatg tgagtgttcg tgaccttgta tcggtcgatc agtcgcaggg
     1081 tctcttcctc gtcccacttc ttcataagga cgacggcgtg tcccgcgtgc agggaagtca
     1141 tcgagaacgt catgacggcc gtgtgataca gcggcgacac gacgacgtgc acgttgtcgt
     1201 tgtaaggctc gatcccgaag agcacgaaga acaacgtgta aagctcggcg gcagtgtcgg
     1261 ggtcgatctg agggaccgca cgacgaacgc ccttgggccg cccggtcgta ccggcggtgt
     1321 agttcatgac ggcaccggtg gcgcgatcct cgggaagcgt gcccggctgt ccttccttta
     1381 tctccgcgta gggacggaag ccgtcgagct tcccgtctac gaagcgtgct tccttcggaa
     1441 gactggcgtc atcaacggct ttcgttgaga cctcgccgaa ctcctcgctc gtgaaaagag
     1501 ccttggcttc ggagtcctcg aggatgtatg cgatctcggg ggccgcgagg tggtggttga
     1561 tcggaacgag gtagaggccc gactgcatgc aggcgagata gagctcgatg agaggcgtgg
     1621 agttcggaag tacggcagcg acggcgtctc cttgcttgac gccgagggcc ctgagtccgt
     1681 ggaccagctt gttcgattcg gcgaacagct cgccggcggt catctcggtg ccgtcgggat
     1741 cgacgagcgc gaggttctca gggtgacgtt gcgctacctc ccagaatccc agctcggcca
     1801 cagtcagaaa attagaacac gttctcatta tgggagcaag ccgaggtgcg cccgttttca
     1861 taacgtgttc taattttggg gaggctgcga aaggagacgg catgagggcg gcgctgctgc
     1921 gagagctgaa cgcggaactc gaggtccgcg acgacttcga ggttaaaggg cccggtcccg
     1981 gcgaggtgcg ggtccgtatc gccgcctcag gcgtctgcca ctccgacctg tcgttgcaga
     2041 acggaacact tccccatccc atcccggccg tgccgggcca tgaaggagcg ggcgagatca
     2101 tcgccgttgg cgaaggcgtt aggggtcttg cagagggcga ccacgtcatc atcacgtggg
     2161 tgccaccgtg cggcgactgt ccttcgtgtg tcgagggtca gccgtacctg tgcggcgcgt
     2221 tcctcgagcc ttcgctgacc gatccgcact tcacttccga cggactggcg gcttttgggt
     2281 tcgccggcac gggaacgttc gccgaggaga ccgtggttcc ccaagaagcg gcgatcaaga
     2341 tcgacaagga catccccttc gacatagccg cgctgatcgg gtgcggcgtg atgacggggg
     2401 ttggtgcttc actcaacacg gcccagatcc gtccaggctc gaacgtcgtt gtgttcggtt
     2461 gcggcggagt ggggatcagc gtgatccagg gagcccggat cgccggcgca gccgagatcg
     2521 tcgccgtcga cgttatggag ccgaagttgg atctggcacg tcagttcggt gcgacacatg
     2581 cctgcttgcc gaacgacctt ccggccatct catccgaggt caccaaggac cggggcttcg
     2641 attatgcact cgaggtcgtg ggacaacccg agacgatccg ggccgcgtgg gatgcgactc
     2701 gccgcggtgg cgagacgatc gtcgtcggtg ccggtgcgat ggacgcgttc gtgagcttct
     2761 ctgcgttcga gctgttctat tcggccaaga ccttgaaggc atgttggtac ggctcgggca
     2821 acgttaggac cgacttccac aagttcctgc ggttctggga tcagggcaag ttggatctcg
     2881 aggggatgat cacccggcgc gtcgacctgt ccgagataaa cgaagcgttc accgcgtgcg
     2941 ccaagggcga actggtccgc gccctagtcg agttcaagta aggagagttc agcgctttct
     3001 cctagaatcg gggtatgtag cgcgccctga cctcacagaa atccatctgg ttagcagaaa
     3061 ggtcagtgat gcgcgctcta cacatcatcg gaggacctcc gggggcgggg aagacgagct
     3121 ccgccggtct cgtggtcgac acctatcagc gaggtgtcca catccggtca gacgacttct
     3181 ggcactacat agcttcggct tatgtcgagc cgtggctccc agcgtcggca gatcagaacg
     3241 ccgtcgtcat gtccgcggtt gcgtcggctg cggcccgatt cgtcattggg aactacgaca
     3301 cgatcgtcga cggcgcgatc gggccgtggt tcctagatcc cttcatcgag gtcgcaaagg
     3361 gatccggctt cgtcctcgac tacgtgatcc ttcgacccga ccgggaagtg gcattagctc
     3421 gtgcccttaa gcgagtcgat ggcggactaa aggaacgagg ccctgtgctg aagatgcatg
     3481 aagagttttc ttcgttggcc gagttcgagc gttacgtcct cgattcgtct gccatgtcgc
     3541 cggagggaac cgcagcggct ctcagatccg ggttagaaga ggggcgattc cgagtgatct
     3601 gaggtcattt gacgtggaag cccatcgtga tctagaggtg ttgcgctagg cgctgaatca
     3661 gcccgtcttt aaccgtgggc cactcggacg cgatcacgga gaacatcgcc gaatcgcgaa
     3721 gacgcccctc ctcgcctggt gctcgcgaaa tcgaccagct gcga
//
LOCUS       DATMED010000399         3913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5692907, whole genome shotgun sequence.
ACCESSION   DATMED010000399 DATMED010000000
VERSION     DATMED010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5692907"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VNP73_10435"
     CDS             complement(<1..526)
                     /locus_tag="VNP73_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66379.1"
                     /translation="MRRCKSLANRLVLAALVSALAAILMVAVGPGLAEQPADPGAQGV
                     ENGQSENAGGSDQSADEASEASDDAAEDTGASETASEDAGSTASDNASENASDNSAVA
                     DASTEEASENSSDAQPENGPPGQTEGDLVSSGAGKSDDASSNGNPKPNEDTSQTGLPR
                     GQEEDECTNKHDTDD"
     gene            complement(638..1756)
                     /locus_tag="VNP73_10440"
     CDS             complement(638..1756)
                     /locus_tag="VNP73_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66380.1"
                     /translation="MNKDLDRLLSESLTSVQESYATERQADRIEARARFIERYRRRRR
                     RLMIGSVALAGAAVIGLGVVATQGFDIFAGPSRVAGQPEDLPKGVLAQIGTGDQAVDA
                     GIQSGGVWVANAGDQTLSRVDAATNEVLDQIELDGPPQEVDVGEGGVWVAGFGRVTAL
                     DPDTGEVLGMADLGSADDSISISVGEGAVWAVVGDRSLYRVDPESFEAQEVAAVSAPV
                     DVAARDGAVWVLDQDQGLLQLDPGTGDAVGEPLPDVPGRGDISAGQGVVWIGNKQDDT
                     LLRIDAATRDISGVFQVTGTYIDMAVSETVIWVLSRTNEDARLTALDPNSNGEVLGTL
                     RISGDPVEVSAGQTGLWVVSRGRGALLRVDADAVLQPN"
     gene            complement(1753..2322)
                     /locus_tag="VNP73_10445"
     CDS             complement(1753..2322)
                     /locus_tag="VNP73_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HWL66381.1"
                     /translation="MRADSLVVDNWIEVGKREHAERDKEIAALFDQHYDGLCDLAFMI
                     MGDRHFAEEIVMEALMKVFTGWGRIRDKARSDVYLKRAVVNLCRSKIRRKMIEARSNA
                     VVYRREERKAPDWDPEVHETSRQVWEAVKLLPPRQRATIVLHYVHALPESEISEILDC
                     SVGTVRSQLSRARGKLAHMLDPSVQGATG"
     gene            2442..>3913
                     /locus_tag="VNP73_10450"
     CDS             2442..>3913
                     /locus_tag="VNP73_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWL66382.1"
                     /translation="MWTKRTPLIFHAALLAISLTLVAGCAARAEPFRPRSASLYDFRD
                     GSAALGVSRDESRTWGATWNDFDSDGDPDLMAGRHWREPLFYVNRGRRFQKLQRDDVF
                     ERVTDRHGCAWGEANGDGLPDLYCVQGADKGTGSGPNQMYVLRAGRFVEEGEGLGVAD
                     ALGRGRTANWLDFDGDMDLDLFVGNHLRPGHPNVTLENTGEGFRRVDIGLSEELSTIG
                     SSWADWDGDSDPDLLLHQLEPNPTVAYMNEGGRFTRVELEGITGLAWKSGAWGDYNGD
                     GWSDLHLVSAHRAVVLRNEGGSFALEHEMALTEGRMSAWLDAENDGDLDLLVVQGAES
                     DDYQPRPGARDNSDFLLVRTRGGFLGPIHQPGSDGPGPNGDAIAVADMNRDGRVDAFV
                     SNGYFHSKGAPTLIENRSRVGHWIGLDLNGTRVNPQGIGASVHVVTPTRTYSRQMTDG
                     VNFRAQSEIGYFHLGISGNRMAFIRVEWPDGTEDCRMALA"
BASE COUNT          685 a         1267 c         1223 g          738 t
ORIGIN      
        1 cgtcgtccgt gtcgtgcttg ttcgtacact cgtcttcttc ctgaccccga gggagccccg
       61 tctgggacgt gtcctcgttg ggcttcggat tgccgttcga gctcgcgtca tcgctcttgc
      121 ccgcgccgct gcttaccaga tcgccttccg tctggcccgg gggtccattt tcgggctgag
      181 cgtcactcga gttctccgac gcttcttcag tcgacgcgtc ggcgaccgcc gagttgtcgg
      241 atgcattctc ggacgcattg tccgaggcgg tgctgcccgc atcctcagat gcggtttcgg
      301 acgcgccggt atcttcggct gcgtcgtctg atgcttcaga ggcctcatcg gcgctctgat
      361 cggatccgcc cgcgttctcg ctctgaccgt tctcaacacc ctgggcacca gggtcggcag
      421 gttgctcggc tagaccgggt ccaaccgcga ccatgagaat tgcggccagc gctgaaacga
      481 gcgctgctag aacgagcctg ttagccaggc tcttacatcg cctcatacac actcctcctc
      541 cattgccagt tcctccactg actaggagag gtatcgggcg atgcagtcgc cttactaaga
      601 actggaagtg actaaatttg cctctagtcc gaggtgacta gttaggctgc agaacggcgt
      661 ccgcgtctac gcggagcagg gccccacgcc cgcgagagac gacccagagg ccggtttggc
      721 cggccgagac ctcaaccgga tcacccgaaa tacgtagcgt gcctaggacc tcgccgttgc
      781 tgttcgggtc gagagccgtg agccgcgcgt cctcgttggt tctggacaga acccagataa
      841 ctgtctcgct gacggccatg tcgatgtagg tccccgtcac ctggaacacg ccgctgatat
      901 cgcgggtcgc cgcatcgatg cgaaggagcg tgtcgtcctg cttgttaccg atccacacca
      961 cgccctgtcc ggccgagatg tcgccgcggc ccggcacgtc gggtaggggc tcccctaccg
     1021 cgtcgccggt accgggatcg agttgcagca gtccctggtc ctggtcgagc acccacacgg
     1081 cgccgtcgcg tgctgcgacg tccaccggcg cactcacggc tgcgacctcc tgagcttcga
     1141 acgattccgg atctacgcgg tacaaagacc ggtccccaac gaccgcccac acggcgccct
     1201 ctccgacgga gatgcttatc gagtcgtcgg cggagccgag atcggccatg cccagtacct
     1261 cgccggtgtc ggggtccaga gcggtgacgc gaccgaagcc tgcaacccac acgccgccct
     1321 cgcctacgtc gacctcttgg ggcgggccgt ctagttcgat ctggtcgagg acttcgttag
     1381 ttgcagcgtc gactcgggac agggtctgat cacctgcatt ggccacccac acccctccgg
     1441 attggatccc ggcgtcgacc gcctggtcac ccgtgccgat ctgcgcgagc acacctttag
     1501 gcagatcctc gggttgtccg gcgacacgcg atgggccggc gaagatgtcg aagccttgcg
     1561 tcgcgacgac acccagcccg atcaccgcgg cccccgccag tgcgaccgat ccgatcatca
     1621 accggcgccg ccgcctgcgg taacgctcga tgaaccgggc gcgagcctcg atccgatccg
     1681 cctggcgctc ggtggcgtac gactcttgaa ccgaggtcaa cgattcgcta agaagacggt
     1741 cgaggtcttt gttcatcccg tcgccccctg cacgctcggg tccagcatgt gggccaactt
     1801 ccctctggcc cgggacagct gggagcggac cgttccgacc gagcaatcga ggatctcgga
     1861 tatctccgac tcgggcagcg cgtgtacgta atggagaacg atcgtcgcgc gctgtcgcgg
     1921 cggtaagagt ttcaccgctt cccacacctg cctcgaggtc tcgtggacct cggggtccca
     1981 gtcgggagcc ttgcgttcct ccctccggta caccaccgcg ttcgaccgcg cctcgatcat
     2041 cttgcggcgg atcttcgatc ggcagaggtt gacgacggca cgcttgaggt agacatcgga
     2101 acgagccttg tcgcggatac gaccccagcc ggtaaagacc ttcataagcg cttccatcac
     2161 gatctcttcc gcgaagtgac gatcgcccat gatcatgaac gcgaggtcac aaaggccgtc
     2221 gtaatgctgg tcgaatagag cagcgatctc tttatcgcgc tcggcgtgct cacgcttgcc
     2281 cacttcgatc cagttgtcta ccaccaggga atctgccctc atcaccctga cagacgcacg
     2341 aggaccccgg caacgttgct cgcctcgccg aaaaatctct gccggcccct tcttcaagca
     2401 atacaagcaa tagaaagggc atcccgagac gtctagtcag catgtggacg aaacgaacgc
     2461 ctctgatctt tcatgcggcc ctattagcga tctcactcac ccttgtcgcc ggctgcgcgg
     2521 cgcgagccga gcccttccga cccagatcgg cgtccctata cgacttccgc gatggctcgg
     2581 cggctctcgg ggtgtcgagg gacgagtcgc gcacctgggg ggccacgtgg aacgacttcg
     2641 attcggatgg agaccccgac cttatggccg gacgtcactg gcgcgagcct cttttctacg
     2701 tgaaccgcgg ccggcgtttc cagaagctgc agcgggacga tgtgttcgag agggtcaccg
     2761 acaggcacgg ctgcgcgtgg ggcgaggcga acggtgacgg tctccccgat ctctattgcg
     2821 tccagggtgc agacaagggc accggcagcg gcccgaatca gatgtacgtc ctgcgggcag
     2881 gtcgattcgt tgaagaaggc gagggcctcg gcgtggccga cgccctcggc cggggccgca
     2941 ccgccaactg gctcgacttc gacggcgaca tggacctgga cctcttcgtt gggaaccacc
     3001 tccgcccggg ccatccgaat gtcacgttgg agaacaccgg cgagggtttc aggagggtcg
     3061 acatcggttt gtcagaagaa ctgtcaacga tcggctcgtc gtgggccgac tgggacggtg
     3121 attccgatcc ggatctcttg ctgcatcaac tggaaccgaa cccgaccgtg gcgtacatga
     3181 acgaaggggg acggttcacg cgcgtggagc tggaggggat caccggcctg gcgtggaagt
     3241 cgggtgcgtg gggcgactac aacggcgacg ggtggtccga tcttcatctc gtttcggcgc
     3301 accgcgcggt cgttcttcgt aacgaggggg gatcgttcgc actcgagcac gagatggcat
     3361 tgacagaagg acggatgagc gcgtggctgg atgcggagaa cgacggcgac ctggacctac
     3421 tggtcgtgca gggggccgag agcgacgact atcagccgcg gccgggggcc cgggataact
     3481 ccgacttcct cttggttcgt acgcggggag gttttctcgg gccgatacac caacccggta
     3541 gtgacgggcc gggcccaaac ggagatgcca tcgcggtggc ggacatgaac cgcgatggac
     3601 gcgtcgatgc tttcgtgagc aacggttact ttcactcgaa gggtgcgcct accctgatcg
     3661 agaaccggtc ccgggtcggc cattggatcg gcctcgatct caatgggaca cgggtaaacc
     3721 cgcaagggat cggcgccagt gtccatgtgg ttaccccgac gcggacctac tcacggcaga
     3781 tgaccgacgg agtcaacttc cgcgctcagt cggagatcgg ctacttccac ctggggatct
     3841 caggcaacag gatggctttc atcagggtcg aatggcccga tggaacggag gactgcagga
     3901 tggcgctcgc cga
//
LOCUS       DATMED010000400         8140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3402108, whole genome shotgun sequence.
ACCESSION   DATMED010000400 DATMED010000000
VERSION     DATMED010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3402108"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /gene="serA"
                     /locus_tag="VNP73_10455"
     CDS             complement(<1..1134)
                     /gene="serA"
                     /locus_tag="VNP73_10455"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWL66383.1"
                     /translation="MRVLVTERLSEAGVERLREHIDVDVKLGLEPEALADEIGAYDAL
                     IMRSATKVTAELIERAENLKVVGRAGIGLDNVDVDAATRHGILVVNAPQSNVLSAAEH
                     TLALLLAQARNVPQAHTALVGGSWEREKWQGIELHGKTLGIVGLGRVGTLVAQRASAF
                     GMRLCAYDPYVTPGRAAQMGVDLVETVGELCRRADFITIHLPKTPETVGIIGERELRD
                     VQPGVRIVNTARGGLIDEDALVDALRDGRVAGAAIDVFEGEPVTEHPLFSLPNVVVTP
                     HLGAATAEAQDKAGLAIAEQVLLALRGEFVPYAVNVDVGTDIPETVRPYLRLAERLGR
                     MAVALAGVGIEGLRFEYHGGIAEYDTRALTLAGLKGAFSAVVHE"
     gene            1177..2130
                     /locus_tag="VNP73_10460"
     CDS             1177..2130
                     /locus_tag="VNP73_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66384.1"
                     /translation="MTDPDGEATSSAPPDELRPLGFGEILERVFKICWSQAKTFLAIV
                     GILIVPANLIFVAAFFVVPPELLAAMEPYSTVEPDIEPYIVRLIAAGVVALVGMLLLM
                     LGTAAATGAAYVAAEASHAGLTITWSQALRTGMRRMFSILWIYFVTGMGLFLSFIIVL
                     APGLAAIVIDQNVGSILAIVFTTLAAFTVSGWLWLSWIFGAAVVMTEEERGTKALRRS
                     YNLIKGRFWWTGGIVFTAMVIASFLGGIFTLPVAFASFTVLGDNIILDLAVRSIANVL
                     GLLLSFPLQITALALLFVDARVVKEGLSKEQLQTEIQSSMR"
     gene            complement(2112..2624)
                     /locus_tag="VNP73_10465"
     CDS             complement(2112..2624)
                     /locus_tag="VNP73_10465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL66385.1"
                     /translation="MHSIRLARTEDAIAIADVHIASWRAGYGELIPADVLDEVLNRRE
                     MERRWNRLLVAPEPPNAQVLLICEGELVFGYSRFGSSRDMDSILDGDTGEVYGFYLHP
                     GRWGRGLGRSLMSAVLEALADQGFRQATLNVIEANERARLFYERQGWTLNTEAVDWYG
                     QPQVRYRIEL"
     gene            complement(2596..3765)
                     /gene="serC"
                     /locus_tag="VNP73_10470"
     CDS             complement(2596..3765)
                     /gene="serC"
                     /locus_tag="VNP73_10470"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HWL66386.1"
                     /translation="MTEPYRDTSDGLGSRAREIVIPPEIRPGDGRFGSGPSKVRPEAI
                     AALYESANSLLGTSHRRAPVKNKVARLKEALTELFELPDGYEVVLGLGGATAFWDAMA
                     FGLIERKSQHLVFGEFSAKCADAARSASHLEEPEVIESEYGTHPEAKASGDVDLYAFT
                     HNETSTGVAMPVARPAPEGIVAVDGVSSAAGLPVDPQDFDVYYFSPQKGFASEGGLWI
                     ALCSPDAIDRIEKLSASGRYVPASLDLAIALDNSRKDQTYNTPALATLFLMVEQVEWF
                     LSQGGMSWSIGRCARSADILYSWAEASPVAIPFVSDPAMRSNVVATIDFEEVDAAQIA
                     AVLRANGIVDTEPYRKLGRNQLRIAMYPAIEPADIELLTRSIDYVVDALDSSRSD"
     gene            3764..4960
                     /locus_tag="VNP73_10475"
     CDS             3764..4960
                     /locus_tag="VNP73_10475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWL66387.1"
                     /translation="MSEQRISRRVAGIAESATLAVDAKAKALKAAGEKVIGFGAGEPD
                     FSTPQHIVEAAAAAASNPVFHKYTPTPGLPELRAAIATKTERDSGYKVGADQVLVSNG
                     GKHALYNAFMSILDPGDEVLVPAPYWVSYPEMIVLAQGEPVVLPTTEATGFRVTLDQL
                     EAAVTDKTKALLFVSPSNPTGSVYPPEEVEAIGRWAVERGLWVVTDEIYEHLTYGDAR
                     HSSMPVAVPELADRCIVINGVAKTYAMTGWRVGWLIGPKDVIVAATNMQSHATSNVSN
                     VAQAAALAAVAGDLAAAEMMREAFDRRRQTIYKLLNEIPGVVCYEPEGAFYAFPSFVD
                     VLGRELRGHTPQSSLELADVILEEVKVAIVPGEAFGAPGYARLSYALGDDDIVEGVTR
                     IADLLA"
     gene            complement(4957..5568)
                     /locus_tag="VNP73_10480"
     CDS             complement(4957..5568)
                     /locus_tag="VNP73_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HWL66388.1"
                     /translation="MGRVERVLESDLYIACLILTGRRALVVGTGPMADEKAQALEACG
                     AEVTRVDPDGYEAGLLDDKLLVVATTGDGELSGDIAAAAEERSMLVNVADVPRLCNFI
                     LPAISRKPPLTVAISTAGASPALAKRMKREVEAMFGSSYVRLAELLDELRPWAKENLD
                     SYEARRDFFESIVNGEPDPIALVKEGREEDLVALIERAKEKAS"
     gene            complement(5622..6791)
                     /locus_tag="VNP73_10485"
     CDS             complement(5622..6791)
                     /locus_tag="VNP73_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66389.1"
                     /translation="MKALKIGILAATLLIVAIAPATAEVEGMKPVGQVKFTGGTELAA
                     SGKYIYAGELNGHTDRNEEPTKGGIHIIDVSKGMKEVGFLHCPGTDNDVEVVKPGLII
                     VSFSQNQCALGAGNGLMTVDVRNPKQPRVLGFVNTGKNHTHKPLPGTSLVYTAGGGLS
                     GGPAAGPAIVDVKNPAKPEVVAKPQTITTDCHDISFHITKDSKLGFCAGAIGTGEVQI
                     WDVSDPLAPVSIGKVVNPAIQYSHYAVASTDGKLLAIDDEAFAAHDCNTGQSPTGRIW
                     IYDISNPATPIPMGSFAAQRGGDGQTNIGTFPGWTTSWCLSHGLDWQPDTHNIAVTWF
                     TGGVSVLNVDTPSTPQEVAYFQAEDSTTYSALWHNGRLYTNDMRRGVDGFEVTAK"
     gene            complement(6838..7995)
                     /locus_tag="VNP73_10490"
     CDS             complement(6838..7995)
                     /locus_tag="VNP73_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66390.1"
                     /translation="MLLGIVAAPAVLAAAEKSDNVKLVAHFPYKNENLDFFSGGTDID
                     FSGDYIYAMQQGADGGVHILDGSGAVPKKVGFFACPGEQNDVAVVKPGLIALGYHESM
                     CGGPGAGVRLLDVKNPKDPKLLGAVNALPGGTHTLTVYPGKPIIYASPGGLPVNGGGV
                     EQILDVSNPAEPKVAATFKPNNSGCHDLSFHFQGKEKLAFCAGAGEVQIWDVTDPLAP
                     VTVGRTVPPTQFAHSVAVTHDGKYMVVGDEAVAGNDCVGGPTGALWIYDITNRAAPIL
                     QGYYRAPRGQQPAGSPDIDRNTWCSAHLFNFVPGTYNLVTSWYSGGMNVIDLTNPAQP
                     AEIAYYMASGEKKEVSNYWSAYWYDGRIYANDRVRGLDVFTVKGLKEGHHH"
BASE COUNT         1439 a         2601 c         2543 g         1557 t
ORIGIN      
        1 ctcgtgcacc accgcactga aagcgccctt caaaccggcc agcgtgagcg cccgcgtgtc
       61 gtactccgcg atccccccgt ggtactcgaa acggagccct tcgatcccga cacccgcgag
      121 agccacggcc atccgtccca accgttctgc aagccgaagg tagggacgaa cggtttcggg
      181 gatgtccgtc ccgacgtcca cgttgaccgc atagggaacg aactctcctc gcagggcgag
      241 cagaacctgt tccgcgatcg caagaccggc cttgtcctga gcctcggccg tggccgcccc
      301 gaggtgcggg gtcaccacga cgttgggcaa cgagaaaagc gggtgttccg tcaccggctc
      361 gccttcgaat acatcgatcg cggcccccgc aacacgaccg tcgcgcaggg catcgacaag
      421 cgcatcctcg tcgatcaatc caccccgagc cgtgttgacg atgcggacgc ccggctgaac
      481 gtcccggagc tcgcgctcgc cgattatccc gaccgtctcg ggtgtcttcg ggagatggat
      541 cgtgatgaaa tcggcccggc ggcaaagttc gccgacggtt tcgacgaggt cgacccccat
      601 ctgggccgct cgccccggcg ttacgtatgg gtcgtacgcg cagagtctca tgccgaaagc
      661 actggcgcgc tgggcaacca gcgtgccgac gcgtccgagc ccgacgatcc cgagtgtctt
      721 gccgtgaagt tcgatccctt gccatttctc tcgctcccat gacccgccca ccagagcggt
      781 gtgcgcttga gggacgttgc gcgcctgcgc aaggagcaac gcaagggtgt gttcggctgc
      841 cgacaacacg ttcgactgcg gagcgttgac taccaagatg ccgtgacgcg ttgcagcgtc
      901 gacgtcgacg ttgtcgagcc cgatccccgc ccgacctacg accttgaggt tctccgcccg
      961 ctcgatcaac tcggcggtga ccttcgtggc cgaccgcatg atgagcgcgt cgtacgcgcc
     1021 gatctcgtcc gccagagctt caggctcgag ccccaacttg acgtcgacgt cgatgtgttc
     1081 tctgaggcgc tctaccccgg cctccgagag ccgctcggtt accaaaactc tcatcgtttt
     1141 ccttctgctt gtttggtgtg tcatcgtaca agcgggatga ccgaccccga cggcgaagcc
     1201 acctccagcg caccacccga cgagctacgt ccgctgggtt tcggagagat cctcgaacgt
     1261 gtcttcaaga tctgctggag ccaggcgaaa acgttcctcg cgatcgtcgg catcctcatc
     1321 gtcccggcga acctgatctt cgtcgccgcg ttcttcgtgg tccctcccga actgctcgcg
     1381 gccatggagc cgtattcgac ggtcgagccg gacatcgagc cctacatcgt gaggttgatc
     1441 gccgccggcg tcgttgcgct ggtgggcatg cttctcctca tgctgggcac tgcggctgct
     1501 acgggggccg cgtatgtcgc cgccgaggcg agtcacgcag gactgacgat cacatggtct
     1561 caagcgctcc gtacgggcat gcgacgcatg ttctcgatcc tgtggatcta cttcgtcacc
     1621 ggaatgggct tgttcctgtc attcataatc gtcctggctc ccggcctcgc agccatagtg
     1681 atcgatcaga acgtcgggtc gatcctcgcg atcgtcttca cgactcttgc cgcgttcacc
     1741 gtttcgggct ggctgtggct gagctggatc ttcggggccg cggtcgtgat gaccgaagag
     1801 gaacgcggga cgaaagctct gcgacgctcc tacaacctga tcaagggacg gttctggtgg
     1861 acgggaggga tcgtctttac ggcgatggtt atcgcttcgt tcttgggagg catcttcaca
     1921 ctgccggtcg cattcgcgtc gttcacagtt ctgggcgaca acatcatctt ggatctcgcg
     1981 gtccggtcca tcgccaatgt gttgggtttg ttgctgtcgt tccccctcca gatcaccgca
     2041 cttgcactgc tgtttgtcga tgcacgagtg gtcaaggaag gcctcagcaa ggaacagctg
     2101 cagactgaga ttcagagttc gatgcggtag cgcacctggg gttgcccgta ccagtcaacg
     2161 gcttcggtgt tgagcgtcca cccctgacgc tcgtagaaga gtcgagctcg ttcgttcgcc
     2221 tcgattacgt tcaacgtcgc ctgccggaaa ccctggtccg cgagggcctc gagcactgca
     2281 ctcatcagcg agcgccccag tccgcggccc caccgaccag ggtgtaggta gaacccatag
     2341 acctcccccg tgtcgccgtc gagtatcgag tccatgtcgc gcgacgagcc gaagcgtgaa
     2401 tacccgaaca ccaactcgcc ttcacagatc aacagaacct gcgcgttcgg cggttccggt
     2461 gcaacgagaa ggcgattcca tctccgctcc atctcgcgac ggttgaggac ctcgtccaga
     2521 acgtcggccg ggatcaactc tccataaccg gcgcgccacg aagctatgtg gacgtcggct
     2581 atcgcgatcg cgtcttcagt ccgagcgaga cgaatcgagt gcatcaacga cgtaatcgat
     2641 cgatcgggtg agaagctcta tgtcagcagg ctcgatcgcg ggatacatgg cgatgcgcag
     2701 ctgattgcga ccgagcttcc ggtacggctc ggtgtcgacg atcccgttcg cgcgcaggac
     2761 ggctgcgatc tgagcagcgt ccacctcttc gaagtcgatc gttgcgacga cattgctgcg
     2821 catggctggg tccgacacaa acgggatcgc gaccgggctc gcctcggccc atgagtaaag
     2881 gatgtcggcg ctgcgagcac aacgaccgat cgaccagctc atgcccccct gggacaagaa
     2941 ccactcgacc tgctcgacca tcaagaacag ggtcgcgagt gcgggggtgt tgtaggtctg
     3001 gtccttgcgc gagttgtcga gtgcgatcgc cagatctagc gacgccggga cataccgacc
     3061 cgaggcgctc agcttctcga tacgatcgat agcgtcgggc gagcacagtg cgatccacag
     3121 tccaccttcc gaggcgaagc ctttctgggg cgagaagtag tagacgtcga agtcctgtgg
     3181 atcgacgggc agaccggccg ccgaggagac accgtccacc gcgacgatgc cctccggagc
     3241 cggccgcgcc acgggcatcg ccaccccggt agaggtctcg ttatgggtga acgcatacag
     3301 gtcgacatcc ccggacgctt tcgcctccgg gtgcgtgccg tactccgact cgatgacctc
     3361 gggctcctcg agatgagacg cgctccgcgc tgcgtcggcg cacttggcgg agaactctcc
     3421 gaagacgagg tgctggctct tcctttcgat cagcccgaac gccatcgcat cccagaacgc
     3481 ggtcgcgcct cccaggccca gcacgacctc gtatccatcg ggcagttcga acagctcggt
     3541 cagggcctcc ttcaaccggg cgaccttgtt cttgaccggg gcccggcgat gactcgttcc
     3601 gagcaaggag ttcgcgctct cgtacagcgc cgcgatcgct tccggccgaa ccttcgaggg
     3661 gcccgagccg aaacgcccgt cgcccggacg gatctccggc gggatcacta tctctcgggc
     3721 gcgactgccg agcccgtcgc tagtgtcccg gtagggttcg gtcatgtccg aacaacgtat
     3781 atcgcggcgg gtcgcgggga tcgcggaatc tgcaacactc gccgtcgacg ctaaagcgaa
     3841 ggcattgaag gccgcaggcg agaaggtgat cggcttcggc gccggcgagc cggatttctc
     3901 gacgcctcag cacatcgtcg aagccgcggc cgccgcggcc tcgaacccgg tctttcacaa
     3961 gtacacgccg acgccggggc tcccggagct gcgtgccgcg atcgcaacca agaccgagcg
     4021 cgattccggc tacaaggtag gggccgacca ggtcctggtg tcgaatggcg gcaagcacgc
     4081 gctctacaac gcgttcatgt cgatcctgga ccccggtgac gaggtactcg tcccggcccc
     4141 ctattgggtc agctatcccg agatgatcgt tctggcccag ggcgagccgg tcgtcctgcc
     4201 gaccacggaa gcgaccggtt tccgggtcac gctcgaccag ctcgaagccg ccgtcaccga
     4261 caaaacgaaa gcactgctgt tcgtgtcgcc gtccaacccg acggggtccg tctatccgcc
     4321 cgaggaggtc gaggcgatcg gtcgctgggc cgtcgagcgc ggtctctggg tcgtcaccga
     4381 cgagatctac gagcacctca cctatgggga cgctcgtcat tcctccatgc cggtcgcggt
     4441 gcccgaactg gccgaccgct gcatcgttat caatggtgtc gcgaagacgt acgcgatgac
     4501 cggatggcgg gtcggctggt tgatcgggcc caaggacgtg atcgttgccg ctacgaacat
     4561 gcagtcgcat gcgacttcga acgtctcgaa cgttgcccag gctgcagctc tagctgccgt
     4621 agccggggac cttgccgcgg ccgagatgat gcgagaggcg ttcgaccgtc gccggcagac
     4681 gatctacaag ctcttgaacg agatcccggg cgttgtctgc tacgagcccg agggagcgtt
     4741 ctatgcgttc ccgtccttcg tagacgtgtt gggacgcgag ttgagagggc acacgccgca
     4801 gagttctctc gagctggccg acgtgatcct cgaagaggtg aaggttgcga tcgttcccgg
     4861 agaggcgttc ggagcaccgg gatacgcgcg tctgtcctac gcactggggg acgacgacat
     4921 cgtcgagggt gtcacgcgca tcgccgacct gctcgcttaa gaagccttct ccttagcccg
     4981 ttcgatcaga gcgacgaggt cttcctccct gccttctttg accagagcga tgggatcggg
     5041 ctcgccgttc acgatcgact cgaagaagtc ccggcgcgct tcgtaggagt cgagattctc
     5101 cttggcccac ggacgaagct cgtccaacag ctcggccagc ctgacgtagg aagaaccgaa
     5161 catggcttcg acctcacgtt tcatgcgctt cgcaagggcc ggggacgcgc cggcagtaga
     5221 gatcgcaacc gttagaggcg gtttgcgcga gatggcggga aggatgaaat tgcacagcct
     5281 cggaacgtcg gcgacgttga ccagcatcga tcgttcctcg gcggcggccg cgatgtctcc
     5341 gctcaactcg ccgtccccag tggtcgcgac cacaagaagc ttgtcgtcga gcaggccggc
     5401 ctcgtagcca tccgggtcga cccgagtgac ctcggcccca catgcctcca acgcctgagc
     5461 cttctcgtcc gccatcgggc cggtgccaac cacgagcgcg cgccggccgg tgaggatcag
     5521 gcatgcgatg tagagatcgc tttcgagcac gcgctcgacc ctacccactg ccccacgcga
     5581 aagggggccg ccgaagcggc cccccggtta gatcagaacg gttatttggc cgttacttcg
     5641 aacccgtcga cgccccgtct catgtcgttc gtgtagaggc ggccgttgtg ccacagcgcc
     5701 gaataggtgg tcgagtcctc cgcttggaag taggcgacct cttgcggcgt gctgggggta
     5761 tcgacgttga ggacgctcac accaccggtg aaccaggtca cggcgatgtt gtgggtgtcg
     5821 ggttgccagt cgagaccgtg gcttaggcac caggacgtcg tccacccggg gaacgtgccg
     5881 atgttggttt gcccgtcgcc accacgctga gccgcgaaac tgcccatggg gatcggcgtg
     5941 gccggattgc tgatgtcgta gatccagatg cgtccggtcg ggctctgacc ggtgttgcag
     6001 tcgtgagcag cgaacgcttc atcgtcgatc gcgagcagct tgccgtccgt agacgccacc
     6061 gcgtagtgcg agtactggat cgcggggttg acgaccttgc cgatcgacac aggggcaagc
     6121 ggatccgaga cgtcccagat ctgaacctcg cccgtaccga tagctccggc acagaaaccg
     6181 agtttcgagt ccttggtgat gtggaacgag atgtcgtggc agtcggtcgt gattgtttgc
     6241 ggcttggcaa cgacctccgg cttggcaggg ttcttcacat cgacgatcgc cggtccggca
     6301 gcaggacctc cggacaatcc gccaccggcc gtgtacacga gcgaggtgcc ggggagcggc
     6361 ttgtgcgtgt ggttcttacc ggtgttgacg aaaccgagaa cgcgaggctg cttggggttg
     6421 cggacatcga ccgtcataag gccgttgccg gctccgagag cgcactggtt ctgcgagaaa
     6481 gacacgatga tcagtccggg cttgacgacc tcgacatcgt tgtcggtacc ggggcagtgg
     6541 aggaatccga cctccttcat ccccttggag acgtcgatga tgtggatgcc acccttggtc
     6601 ggttcctcgt tgcggtccgt gtgaccgttc agctcacccg cgtagatgta tttcccagac
     6661 gcggcaagtt cggtaccgcc ggtgaacttg acctgaccga cgggcttcat cccctcaacc
     6721 tctgcggtcg cgggagcgat cgcgacgatg agaagcgtcg ccgccaggat cccaatcttc
     6781 agcgccttca tctggtgaac tccttcgttc gttgatcttt ggctagtggt tctctctcta
     6841 gtggtgatga ccttccttga ggcccttcac cgtgaagacg tcgaggcctc ggacccggtc
     6901 gttcgcatag atgcggccgt cgtaccagta ggcagaccag tagttcgaga cttccttctt
     6961 ctcacccgac gccatgtagt acgcgatctc ggccggctgg gccgggttgg tgagatcgat
     7021 cacgttcatg ccgcccgagt accaagaggt gacgaggttg taggtgccgg gcacgaagtt
     7081 aaagaggtgg gccgaacacc aggtgttgcg gtctatgtcc ggcgagcctg ccggctgctg
     7141 accgcgcggt gcacggtagt agccctgcag gatcggagcc gcacggttcg tgatgtcgta
     7201 gatccacagc gccccggtcg gtccgccgac acagtcgttg ccggcgaccg cctcgtcgcc
     7261 gacgaccatg tacttgccgt cgtgcgtaac cgcgaccgag tgtgcgaact gagtgggcgg
     7321 aacggttcta ccgaccgtca ccggcgcaag cggatcggtc acgtcccaga tctgcacctc
     7381 accggccccg gcacagaagg cgagcttctc tttgccctgg aagtggaacg agagatcgtg
     7441 gcaacccgag ttgttcggct tgaacgttgc cgcaaccttc ggctctgccg ggttgctgac
     7501 atcgaggatc tgctcgacgc ctccaccgtt aacggggagg ccacccggag atgcatagat
     7561 gatcggcttg cccgggtaaa cggtgagcgt gtgcgtcccg cccgggagag cgttgaccgc
     7621 tcccagcagc ttcggatcct tcgggttctt gacgtcgagc aaccggacgc cggcaccggg
     7681 cccaccacac atgctctcgt ggtagccgag tgcgatcagg ccgggcttga cgacggcaac
     7741 gtcgttctgc tcgcccggac aggcgaagaa accgactttc ttgggcaccg cgccggagcc
     7801 gtcgagaatg tgaacgcctc cgtcggcccc ctgctgcatc gcatagatgt agtcaccgga
     7861 gaaatcgatg tcggtgccgc ccgagaagaa gtcgaggttc tcgttcttgt aagggaagtg
     7921 cgcgaccagc ttcacgttgt cggacttctc ggcggccgca agaacagcag gagcggcaac
     7981 gatgcccagg agcaaggctg ccgccaagct catcgcagtc aacctacgaa ccgtcttcaa
     8041 tgacgtttct ccttgtgagt ggcagaaaag tgtcttggag ggatggttcg ctgccgtctc
     8101 ggacagtcct gcacgcctag cagtcgtcga gggccttgcc
//
LOCUS       DATMED010000401         4920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3015118, whole genome shotgun sequence.
ACCESSION   DATMED010000401 DATMED010000000
VERSION     DATMED010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015118"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..908
                     /locus_tag="VNP73_10495"
     CDS             162..908
                     /locus_tag="VNP73_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HWL66391.1"
                     /translation="MTTPAPTVTDHDDFERQLLELRKREKAHTREGDTIAAARRRLPM
                     TEVPPDTTVIGSDGPVPFGDVFEGREELVIYSHMFYDGKPWEWQCEGCTRNSWNMGDA
                     ADAAYLNANGITFAFLCDGPYEEIAAFRDFMGYTAPWYSNAHLDDPTVGVAGRIACYL
                     RRVDKIYLTYWTTGRGDEVLSPSFGLLDMTAYGRREEWEDSPEGWPQFSTHSILRTDE
                     HGAPMGPRKGGRPVPQWTLPGVSGLGEQTG"
     gene            complement(938..3763)
                     /locus_tag="VNP73_10500"
     CDS             complement(938..3763)
                     /locus_tag="VNP73_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL66392.1"
                     /translation="MSARGILPIALSAVLIAAAAGVATAGAAVTPKCRGKKATIVGTN
                     GADDLEGTKKVDVIVALGGDDRIEGGTGTRDGGLIDLICGGAGNDTFSDGGNYWGKFD
                     GGPGTDTMTGNSTILLGNLGNSGSYGDTANVPLYNVENAIGTPLGDTLAGGPGTNVLT
                     GGGGPDWFLYYPADTGDDIWDGGEGDDRADYTRAQGAVSADLGGGTASIAGYGTDSLV
                     GIDDITGSYFADMLTGSDLPNNMQGANTGVSQNHDFAGDELSGLGGDDFLRASAGPVT
                     MHGGDGNDQLAALGDAGQMLDGGDGNDIIGGSQYVHTGTSIYVGGPGDDWIDGSGGND
                     VMSGDAGADLIEASYGDDTIDGGANQDTADGGRGRDTCSNVESFRGCASGEFDDFDIG
                     DPLTPSRSSDVQLAVALDEDPTGKKAVYEFTVTNHGPDSLDSPALKVHVAGIVDLPDG
                     CVGDGPTGATCGPGALDAGEQFRIKLDVTQPLDIAHVAVAIPAGPHDPNWSNNVATFG
                     GPPPPPVCQVDPPPAGEIDLTKRELTEVLRDERNNLPLGKWIDRLEAAIAAIEADATP
                     DPDQHAENEAVLAELNALLDEATAQRSPRVSLRMQEDDGLSDNERQLLENAACGALDE
                     AKSSLETYLEENVSEDHGALFNKLDTLADILNAEEPTDEEEQEYLGAAIEDLVARLAK
                     DKLDGKTDEEIAEFTGPYKDFSLAAFDILKDGLSGELGPNKLKHLEATLKAASDAFGS
                     SDTTEFIELSFKFLTVIRGEATPQEEYEILKETVANLANRLTTRIFGTAILSTPQARA
                     AMLGFQIGLALGERIARDLELIFKGSLVEDCTKALATAQGKSNPVDVDYSRPDFGIVS
                     KTSGGIIPWHLGWRCDIVDNGHTRSRAGGVVQATRPTDEEFEAACEEADGYVSCNAYY
                     VVWRITTHGRGVILWDPACAIARGCRF"
     gene            complement(3928..4107)
                     /locus_tag="VNP73_10505"
     CDS             complement(3928..4107)
                     /locus_tag="VNP73_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66393.1"
                     /translation="MKRLRVLVAATFAVSVLSIVVAQPAYAVVCQEQPDGSCQCGDPV
                     NRVWEKLFGGPLIVC"
     gene            complement(4203..>4920)
                     /locus_tag="VNP73_10510"
     CDS             complement(4203..>4920)
                     /locus_tag="VNP73_10510"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HWL66394.1"
                     /translation="GAFHDVYRKYSMRDRLIREHGTAANQVFWEGQTPLLGDSTFVDD
                     SIRKMDEWLAAIQADTSDLPLSQKIIDAKEPAGVTERCVGMGGQDVPLQMCDVTVDAT
                     IYSSPRIEAGGGDPALVNGVGPATVGFADDRLACDTMPVEDFVYAGKSFVEAFTPVQQ
                     ESLKQVFATGVCDYSKPGIGFQETVPWLTYQDDAGNVIYGGVPLGDPPLSEYVAPTTT
                     RKRPRPTPSGPCRKPSKPCA"
BASE COUNT          851 a         1615 c         1522 g          932 t
ORIGIN      
        1 gcgcatgctg cttgtccgtc tcgacgctgc ccgccggcat ggctcctcca ccgaacgcga
       61 ggaacacggt gatcaagccg gcaagagctc tctgcatcgt ccgctccctg atgagtttcc
      121 ggtaatgatc cagtctaagt aatgagagct aggagggaca catgaccacg ccggccccga
      181 ccgtcaccga ccacgacgac ttcgagcgac agctcctcga gctgcgcaag cgcgagaaag
      241 cacacacccg cgagggcgac acgatcgccg cggcccggcg ccggctgccc atgacagagg
      301 tgccgcccga caccaccgtc atcggctccg acggcccggt cccgttcggc gatgtcttcg
      361 agggacgcga agagctggtc atctacagcc acatgttcta cgacgggaag ccatgggagt
      421 ggcagtgcga gggctgcacc aggaactcct ggaatatggg ggacgccgcc gacgcggcct
      481 acctcaacgc caacgggatc accttcgcct ttctctgcga cggaccgtac gaggagatcg
      541 cggcattccg cgacttcatg ggatatacgg cgccgtggta ctccaacgct cacctcgacg
      601 accccaccgt gggcgtcgcc ggtcggatcg cctgctacct gcggcgggtg gacaagatct
      661 acctgacgta ctggacgacc ggccggggcg acgaagtgct tagcccctcg ttcgggctac
      721 tcgacatgac cgcctacggg cggcgggagg agtgggagga ctcgcccgag gggtggccgc
      781 agttctcgac ccactccatc ctgcgcaccg acgagcacgg cgcgccgatg gggcccagaa
      841 agggtggtcg accggtgccc cagtggacgc tcccgggggt cagcgggctc ggcgaacaaa
      901 ccgggtagca ctgccactga ggcgaggtgg ccggaggtta gaagcggcaa cctcgcgcta
      961 tcgcgcacgc cggatcccaa aggatgacgc ccctgccgtg agtcgttata cgccagacga
     1021 cgtagtacgc gttgcacgaa acgtacccgt cggcttcttc gcacgcggcc tcgaattcct
     1081 catcggtggg tcgggtcgcc tgcaccacgc ctcccgctcg actccgagtg tgcccgttgt
     1141 cgacgatgtc acagcgccag ccgaggtgcc acgggatgat ccctcctgag gttttcgaca
     1201 cgatcccgaa gtccggcctg ctgtagtcga cgtcgacggg attcgatttg ccctgcgcgg
     1261 tcgcgagggc tttcgtgcag tcctccacga gactcccctt aaaaatcaac tcgaggtcgc
     1321 gtgcgatgcg ctcgccgaga gcgagcccta tctggaaccc gagcatggcc gcccgcgcct
     1381 gaggtgtcga gaggatcgca gtgccgaaga tccgcgttgt aagccggtta gccaggttgg
     1441 cgaccgtctc tttcaggatc tcgtactcct cttgcggtgt ggcttcgcct ctgatgactg
     1501 ttaggaactt gaaactcagt tcgatgaact cggtcgtgtc gctgctgccg aacgcgtctg
     1561 aagccgcctt aagcgttgct tccaagtgct tgagcttgtt cggaccgagc tcgccggaaa
     1621 gaccgtcctt caagatatcg aaggctgcca gcgagaagtc cttgtaaggc ccagtgaact
     1681 cggcgatctc ttcgtcggtc ttgccgtcga gcttgtcctt cgccaaacga gcgacgagat
     1741 cctcgatcgc tgcaccgagg tattcctgct cttcctcgtc ggtgggctcc tcggcgttaa
     1801 ggatgtccgc cagtgtgtcg agcttgttga acagcgctcc gtgatcctcg ctgacgttct
     1861 cctcgaggta cgtctcgaga gatgacttcg cttcatcgag cgcgccgcac gcagcatttt
     1921 cgagcagctg tcgctcgttg tccgacaggc cgtcgtcttc ctgcatgcgc aacgacaccc
     1981 gcggcgatcg ctgggccgtc gcctcgtcca gcagcgcgtt gagttccgca agcaccgctt
     2041 cgttctctgc atgctggtcg gggtctgggg tcgcgtctgc ctcgatcgcc gctatcgctg
     2101 cctcgagcct gtcgatccac ttgccgaggg gcagattgtt ccgctcatct cgtagaacct
     2161 cggtgagctc tcttttcgtg agatcgatct ctcccgccgg cggaggatcg acctggcaca
     2221 ccggcggagg aggcgggccg ccgaaggtcg caacattgtt ggaccagttc ggatcgtgag
     2281 ggcccgcagg gatggcgacc gcgacgtgcg cgatatccag cggctgcgtg acatcgagtt
     2341 ttatccgaaa ctgttctccg gcgtccaggg ctccaggccc acaggtggcg ccggtcggac
     2401 catccccgac acagccatca gggagatcca cgatgccggc aacgtgaacc ttgagggccg
     2461 gagagtccag gctatcgggg ccgtgattgg taacggtgaa ctcgtaaacg gctttcttgc
     2521 ccgtcgggtc ctcgtcgaga gcgacggcta gttggacatc cgacgaccgg ctcggagtca
     2581 ggggatcgcc gatgtcgaaa tcgtcgaact ccccgctcgc acatccccgg aagctctcga
     2641 cgttggagca ggtatcgcga ccacgtccac cgtccgcggt gtcttgattg gcacctccgt
     2701 cgatcgtgtc gtcaccgtat gaagcctcta tcaggtcggc ccccgcgtct cccgacatca
     2761 catcgttgcc gcccgatccg tcgatccaat cgtcgcccgg cccccccaca tagatactgg
     2821 ttccggtgtg gacgtactgg gatcctccga tgatgtcgtt gccgtccccg ccgtccaaca
     2881 tctgacctgc gtcgcccaaa gcagccagtt ggtcgttacc gtccccaccg tgcatcgtga
     2941 ccggacctgc gctcgcgcgg aggaagtcat ctccaccgag cccgctgagc tcatcgcctg
     3001 cgaagtcgtg gttctgggac acgccggtgt tcgccccctg catgttgttt gggagatcgg
     3061 accccgtcag catgtccgcg aagtagctac ccgtgatgtc gtcaatcccc acgagcgagt
     3121 cggtgccgta gccggcgatc gaagccgttc cgcctccgag atcggccgat acagcgcctt
     3181 gcgcgcgcgt gtagtcggca cggtcgtctc cctcaccgcc atcccatatg tcgtcgccgg
     3241 tatccgcggg gtagtacaga aaccagtcgg ggccaccgcc cccggtaagt acgttggttc
     3301 cgggtccgcc ggccaaggtg tcaccgagcg gggtcccgat cgcgttctcg acgttgtaca
     3361 ggggaacatt ggcggtgtca ccgtaagaac cggagttgcc caggtttccg agaaggatgg
     3421 tggagttacc tgtcatcgtg tcggtgcccg gtccaccgtc gaacttgccc cagtagttgc
     3481 cgccatccga gaaggtgtcg ttgccggctc cgccgcagat gaggtcgatc aacccgccat
     3541 cacgtgttcc ggttccgcct tcgatcctgt cgtcaccgcc gagggcaacg attacgtcga
     3601 cctttttggt tccctcgagg tcgtcggcac cattcgtccc gacgatggtc gccttcttgc
     3661 cgcgacactt cggggtcacg gctgcgccgg cggtcgccac gccagcggcc gccgcaatca
     3721 agacggcaga cagcgctatc ggaagaatcc cccgggcaga catctgcccc agaagtatcg
     3781 aaccaggagt tcgcggtcac aagagggttg ggccagacct aggccccaaa tcgccaccgg
     3841 ggttcgcgta caaaccgggt tgccctgccc tgaggcgagt gagatcaaag accggaggcg
     3901 cctcttggtg aagttgcccc gaaggagcta gcaaacgatg agcggcccgc cgaagagctt
     3961 ctcccacacg cggttgacag ggtccccgca ctggcagctc ccgtcgggct gctcttggca
     4021 gaccaccgcg taggcgggct gggccaccac gatcgacaga accgacacgg caaaggtcgc
     4081 cgccacgaga acgcgcaaac gcttcattga ttcctccttc ggtgaactac cggctggctg
     4141 agcaatacga cgtccggtcg ccaaaaacct tcctgctcgg ccggcaaagc ttctgagccg
     4201 gatcatgcac aaggcttact gggctttcga cacgggccgc ttggggtcgg ccgagggcgc
     4261 ttccgggtgg tcgtgggggc gacgtattcg gacagcggcg gatcgccgag cgggacgcct
     4321 ccatagatga cgttgccggc gtcatcctga tacgtcagcc agggaacagt ctcctgaaag
     4381 ccgatgccgg gtttgctgta gtcgcaaact ccggtcgcga aaacctgctt caggctctcc
     4441 tgctggaccg gcgtgaatgc ctcgacgaac gacttccccg cgtagacgaa gtcctcgacc
     4501 ggcatcgtgt cgcatgccag ccggtcgtcg gcgaaaccaa cggtcgccgg gccgaccccg
     4561 tttaccaagg ccggatcgcc tccgcccgcc tcgatccgcg gtgacgagta gatcgtggcg
     4621 tcgacggtga cgtcacacat ctgtagtggt acgtcttggc cgcccatgcc gacgcaacgc
     4681 tcagtgactc cggcgggttc ctttgcgtcg atgatcttct gggagagtgg gagatcgctc
     4741 gtgtcggcct ggatggcggc gagccactcg tccatcttcc tgatcgagtc gtccacgaag
     4801 gtcgagtcgc ccaagagcgg cgtctggcct tcccagaaca cttggttcgc cgccgttccg
     4861 tgctcgcgga tcagacggtc gcgcatcgag tacttgcgat acacgtcgtg gaaggcaccc
//
LOCUS       DATMED010000402         2630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_700179, whole genome shotgun sequence.
ACCESSION   DATMED010000402 DATMED010000000
VERSION     DATMED010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700179"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VNP73_10515"
     CDS             <1..361
                     /locus_tag="VNP73_10515"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWL66395.1"
                     /translation="GEKISQHRANVYLVNTGWTGGPYGIGRRMDLTHTRRMVSAATSG
                     QLEDVDTKLHDIFNLEVPVECPGVPADVLDPRSTWSDKAAYDKQARELAEMFVKNFER
                     FARDVSDDVVKAGPIVG"
     gene            365..1330
                     /gene="thiL"
                     /locus_tag="VNP73_10520"
     CDS             365..1330
                     /gene="thiL"
                     /locus_tag="VNP73_10520"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWL66396.1"
                     /translation="MATVAELGEFALIDRWRTRIGSAPENEVWSGDDAAVIASPGDRL
                     VLTTDVLVEGIDFDLRYAEGPDIGWKAMASNLSDVAAMGGRPRRAVATLTLRPDTSVG
                     LVDGILEGMLELSGRWDVGLVGGDVSGSPTLSLGVTVVGVAEIPLLRSGARIGDVLCV
                     TGELGGAAGGLLALKDGRLDMSGLIERQLRPAPRVAEGVALAQAGASAMIDVSDGLAA
                     DLDHLVSPSGVGCEVDIESIPVDPALAGVADLIWPMEAALTGGEDFELLVALPEERVG
                     DATEALTDLGTVLTRIGRVTQGAARIGSNSISEWRDKGWEHLRRP"
     gene            1306..2136
                     /gene="thiD"
                     /locus_tag="VNP73_10525"
     CDS             1306..2136
                     /gene="thiD"
                     /locus_tag="VNP73_10525"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWL66397.1"
                     /translation="MGTPATPVTQLPRALTIAGSDSGGGAGIQADLKVFFALGCHGMS
                     ALTALTAQNTVGVDGVHEVPADFVTDQIDSVASDIGVDAAKTGMLSSSPIVDAVATAI
                     KGHGIAALVVDPVFVSKHGHHLLAESAVDALKSRLFPLAEVITPNLYEAAALTGREIV
                     TVDAMKQAARELHDLGPRAVLVKGGHLAGAEAIDVFYDGDRVAELSGPRFETEDTHGT
                     GCALSAAIAARLAHGDDLFDAVTFAKEFVAGAIRNSLRIGRGYGPVNPGWRARSNTTS
                     "
     gene            complement(2085..>2630)
                     /locus_tag="VNP73_10530"
     CDS             complement(2085..>2630)
                     /locus_tag="VNP73_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66398.1"
                     /translation="ALVPLGVLLQLLITQLATSVALVVATRSLGSGEPFRLKPLGPAC
                     GAGAVAAAVTIVIALIPLAGLLIVLVFSLGLVGPPLVAQAIAVEDLGLKPSLKRAGEM
                     ARRFPTGLAAIVGVSAALGVISLVLLGAALAALADAPSAMRTVVISGVQPLVLGSALA
                     YLAALQLVVFERARQPGLTGP"
BASE COUNT          476 a          796 c          926 g          432 t
ORIGIN      
        1 gggcgagaag atctcgcagc accgagcgaa cgtctatctc gtgaacacgg gctggacggg
       61 aggtccgtat gggatcggca ggaggatgga tctgacgcac accaggagga tggtgtctgc
      121 ggcgacgagc ggacagctcg aagacgtcga tacgaagctt cacgacatct tcaacctcga
      181 ggtgcctgtg gagtgccccg gagtgccggc cgacgtcctc gatcctcgct ccacctggtc
      241 ggacaaggcg gcttacgaca agcaagcccg ggagctggcc gagatgttcg tcaagaactt
      301 cgagcggttc gctcgggacg tatccgatga cgtcgtcaag gcggggccga tcgtcggctg
      361 atccatggcc accgttgccg agctcggcga gttcgcactg atcgatcgct ggcgcacgag
      421 gatcggcagc gcgcctgaga acgaagtgtg gtcgggcgac gatgcggcag tgatcgcgtc
      481 tcctggggac cggctcgttc tgacgacgga tgttctcgtc gaggggatcg acttcgatct
      541 gcgctacgcc gagggtcccg acatcggctg gaaggccatg gcatccaacc tctccgacgt
      601 ggcggcgatg ggtggccggc ccaggcgcgc agttgcgact ctcactttgc gccccgatac
      661 ctcggtgggg ctggtcgacg gcatcctcga agggatgctg gaactaagcg ggcggtggga
      721 cgtcggtctc gtgggcggcg acgtcagcgg ttcgccgacg ttgagcctcg gggtaacggt
      781 tgtcggtgtt gccgagatac ccctcctgag atcgggtgcc cggatcgggg acgttctgtg
      841 cgtgacgggg gagctcggcg gcgcggcggg gggattgctg gccctgaagg atggccgttt
      901 ggacatgagc ggtctgatcg agcgccagtt acgcccggcg cccagggtgg cggagggagt
      961 cgcgctcgcg caggcaggag cttcggcgat gatcgacgtg tccgatggat tggctgcaga
     1021 cctcgaccac ctggtgagcc cgagcggcgt ggggtgcgag gtcgacatcg aatcgatccc
     1081 ggtggatccc gcccttgccg gtgtcgccga cctcatctgg ccgatggaag ctgctctgac
     1141 cgggggagag gacttcgagc tgttggtggc acttcccgaa gagcgggtcg gggatgcaac
     1201 cgaggccctc accgatctgg gaacggtgct cacccggata gggcgggtga cacagggagc
     1261 ggcgcggatc gggtcgaatt cgatctccga gtggagggac aagggatggg aacacctgcg
     1321 acgcccgtga cccaacttcc gcgcgcgctg acgatcgcag gctcggattc tggtgggggg
     1381 gccggtatcc aagccgacct caaggtcttc ttcgcactgg gatgtcacgg catgagcgct
     1441 ttgaccgcgc tcacggctca gaacaccgtg ggcgtggatg gggtgcacga ggtacctgcc
     1501 gacttcgtga ccgatcagat cgactcggtt gcctcggata tcggcgtaga cgccgccaag
     1561 acggggatgt tgtcgtcgag cccgatcgtg gacgcagtgg caaccgccat aaagggtcac
     1621 ggcatcgcgg cgctcgttgt cgacccggtg ttcgtgtcca aacacggcca tcacctgctc
     1681 gccgagtctg cggtagatgc gctgaagtcg cgtctcttcc ccctcgccga ggtcatcact
     1741 ccgaacctct atgaagccgc tgcattgacc ggccgagaga tcgtgacggt cgatgccatg
     1801 aaacaagcag ctcgcgagct tcacgacctc ggcccccgag ccgtactcgt gaagggaggc
     1861 cacctggccg gagccgaggc gatagacgtg ttctacgacg gcgatcgcgt cgccgagctc
     1921 tccggtcctc ggttcgaaac cgaggacacg cacgggacgg gatgtgcgtt atccgccgcc
     1981 atcgccgctc gtctggctca cggcgacgac ctgttcgacg cggtgacgtt cgctaaagaa
     2041 ttcgtcgccg gcgcgatccg caatagcttg cgcatcgggc gtggctatgg tccggtcaac
     2101 cccggctggc gagcgcgttc gaacacgacc agttgaagcg ccgcgagata cgcgagggcg
     2161 ctaccgagca cgagcggctg aacaccggag atcacaacgg tgcgcatcgc gctgggtgca
     2221 tcggctagag ctgcgagcgc ggcgccgaga agcaccagtg agatcacgcc gagcgcggca
     2281 ctaaccccga cgatcgcggc gaggcccgtc gggaaccttc gggccatctc gccggcgcgc
     2341 ttcaacgatg gtttgaggcc cagatcctca acggcgatcg cctgagcgac cagaggcggt
     2401 ccgaccagac cgagtgagaa cacgaggacg atcaacagtc cggcgagggg gatcaacgcg
     2461 atgacgatcg tcacggcagc tgccactgca cccgctccac aggccggccc cagtggcttg
     2521 agacggaaag gctcacccga tccgagcgag cgcgtggcga cgaccaaggc aacggaagtg
     2581 gccaactgcg ttatcagcag ttgcaacaga acgccgagcg gcacgagcgc
//
LOCUS       DATMED010000403         2933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1077408, whole genome shotgun sequence.
ACCESSION   DATMED010000403 DATMED010000000
VERSION     DATMED010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077408"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VNP73_10535"
     CDS             complement(<1..913)
                     /locus_tag="VNP73_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWL66399.1"
                     /translation="MAGALATMPGSLARLWLAVASAASGALVGLGDAPVLGAWVVMAA
                     GGSLLALRRQRTAVLIGLALLCASVSWVLAGMQTHEDPALALLGTSVPRCSFAGHVLE
                     QAGGLGTLAEIHARDCTPPGAAGGVMILDEGEGLASGAPISGEGWLLPLGRDGFDRAR
                     RNLGAQVLLDPIELHVDPPRSTVLGLAHVMRSGLKRSVEGLGEQEGALLLGLTIGDTS
                     RFDPEVTEAFRRSGLAHLLAVSGSNVALVLASIALALRRFSRRTRVVGGACTLCLYVS
                     LVGPDASVLRAALMGGVTLVAVGWAGRV"
     gene            complement(1062..1688)
                     /locus_tag="VNP73_10540"
     CDS             complement(1062..1688)
                     /locus_tag="VNP73_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HWL66400.1"
                     /translation="MHDLTGSGDRRARWEELAGKRRDVWLIAAIVVALLLGALVFKAI
                     RLRPQIAPPASATHSVPTATASGAAILYVHVSGAVRKPGLYEFLPGARVADAVETAGG
                     AKPGADLSALNLAELLTDGMQVDVPARGNGSAAPVATASGASPATVNLNTADQIALET
                     IPGVGPVTALAILQTRDRLGSFTTFEQLLEVDGIGPATLEAMRPYVSI"
     gene            1754..2155
                     /gene="mscL"
                     /locus_tag="VNP73_10545"
     CDS             1754..2155
                     /gene="mscL"
                     /locus_tag="VNP73_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWL66401.1"
                     /translation="MLNEFRNFLLRGNVVDLAVAVVVGTAFGAIVTSLVTDILTPLLG
                     LLGIPDFGHLERGIGPSTIRYGVFLNALISFVTVSAAVFFFVVKPINKLMERRKTQPD
                     VENPTKQCNECLSNIPVGARRCAFCTSQVAA"
     gene            complement(2180..>2933)
                     /locus_tag="VNP73_10550"
     CDS             complement(2180..>2933)
                     /locus_tag="VNP73_10550"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWL66402.1"
                     /translation="MGGKRMSKSRGNIVEPQEAFERFGADSLRLYMLFSGPPEAPFDW
                     PEEGVLAIGRVTFPWLERVWRLCEETGELVQTAEKGFAPADTELRKLVHRTIKVVTDD
                     FESFSFNTAIARLMELVNGAYKYRSNGGGHPEVMRELVEALLKLLAPMAPYITEEQWR
                     RLGHEDSIHRQSWPQHDPELAAEEEVTMVVQVNGKVRDTIRVPVDITEDKMKALALAS
                     EKARAHLDGGDPEKVIVRPPKLVNLVKRSFAQ"
BASE COUNT          531 a          974 c          928 g          500 t
ORIGIN      
        1 cgacgcgccc ggcccatccg accgccacca gtgtcacgcc tcccatcaaa gccgctcgca
       61 gcaccgaggc gtcgggtccc acgagcgaca cgtagagaca gagggtgcag gcaccgccca
      121 cgacgcgcgt cctgcgggag aacctccgga gcgccaaagc gatgctcgcc aggaccaagg
      181 cgacgttgct tcccgatact gcaaggagat gtgcaagacc cgaccggcgg aacgcctcgg
      241 tgacctcggg atcgaatcgg gacgtgtcgc cgatcgtcaa accgaggagg agggctccct
      301 cctgctcccc tagtccctcc acgcttcgct tcagccccga acgcatcacg tgagcgagtc
      361 ccaggaccgt tgatcgtgga ggatcgacat gcaactcgat cggatccagt aggacctgag
      421 cacccaggtt cctgcgcgcc cggtcgaatc catctcgacc caggggaagc agccagccct
      481 cacccgagat cggagctccc gatgcgaggc cctctccttc atcaaggatc atcacccctc
      541 cggctgcacc cggaggggtg caatcgcgcg catgtatctc ggcaagggtt ccgaggcctc
      601 ccgcttgctc gagcacgtga ccggcgaacg aacaacgagg caccgacgtg ccgagcagtg
      661 cgagagccgg atcctcgtgc gtttgcatcc cggcaagcac ccacgacacg ctcgcgcaca
      721 agagcgccaa gccgatgaga accgctgtgc gttgccgccg cagcgcaaga agagaaccgc
      781 ccgccgccat cacgacccac gcaccaagaa caggggcatc gccgagaccg accaacgcgc
      841 ccgacgcggc ggatgcgacg gcgagccaca gcctggcaag cgaacccggc atcgtcgcta
      901 gtgccccggc catgaatgtt tgcgaggttc tggcgagtga aaagccagcc tcgtaaggcc
      961 gcagggagcg tgcgtacctg gggggtacgt gcgcgaccga gaacgccgcg aggcgcagcc
     1021 ttttcgccgc cagaatccgg aggatatttg tggccggggc actagatgct cacgtagggc
     1081 cgcatcgcct cgagggttgc gggcccgatc ccgtcgacct cgagaagctg ctcgaaggtg
     1141 gtgaaggaac ccaatcgatc ccgcgtttgc aggatcgcaa gggcggtaac cggtccgacg
     1201 ccggggatcg tctccaatgc gatctggtcc gcggtgttca ggttcaccgt agccggggac
     1261 gcgcccgaag ctgtcgcgac gggcgcggcg gaaccgttac cgcgagccgg cacgtcgacc
     1321 tgcatgccgt cggtgagcaa ctcggcgagg ttcaatgcgg agaggtcggc cccgggcttt
     1381 gccccgccgg ccgtctcgac cgcgtcggcg actcgcgcgc ccgggaggaa ctcgtacagc
     1441 cccggtttcc gtaccgctcc cgaaacgtgc acgtagagga tcgccgcacc cgacgcggtt
     1501 gcggtaggaa ccgaatgagt tgcagacgcc ggtggcgcga tctgaggtcg cagcctgatc
     1561 gctttgaaga cgagggctcc gaggagcagt gcaaccacga tcgctgcaat cagccatacg
     1621 tcccttcgct taccggccaa ctcctcccac cgtgcgcgcc ggtccccact ccccgtaagg
     1681 tcgtgcatga agggaatcca agcgggcagg ctccgggcgg gctgtgccgc agcacacaac
     1741 caggagggtt tggatgctca acgagttcag gaatttccta ttgcggggga acgtcgtaga
     1801 tctggccgtg gcagtcgtcg tggggaccgc cttcggtgcg atcgtgacgt ctctcgtcac
     1861 cgacatactg actccgcttc tcggattgtt ggggatcccc gacttcggcc acctcgagag
     1921 aggcatcggt ccctccacga tccggtacgg agtgttcctg aacgcgctga tctcgttcgt
     1981 gacggtctcg gctgcggtct tcttcttcgt cgtcaagccg atcaacaagt tgatggagcg
     2041 ccggaagact cagccggatg tcgagaaccc cacgaaacaa tgcaacgagt gcctcagcaa
     2101 catcccggtc ggcgctcgtc gatgcgcctt ctgcaccagc caggtcgcgg cctagctcaa
     2161 cgggtgggcc ggggggcgat cactgagcga agctcctttt caccaggttc actagcttcg
     2221 ggggtcgcac gatcaccttc tcggggtcgc ccccgtcaag atgcgcgcgt gccttctccg
     2281 aggccagtgc gagagccttc atcttgtctt cggtgatgtc gaccgggacg cggatcgtgt
     2341 cacgcacctt cccgttgacc tggacgacca tcgtgacttc ctcttcggcg gccagctccg
     2401 ggtcatgctg cggccaactc tgtctgtgga tcgagtcctc gtgcccgaga cggcgccatt
     2461 gctcctccgt gatgtagggg gccatcggcg ccagcagctt cagtagcgcc tcgaccagtt
     2521 cgcgcatcac ttcggggtgt cccccgccgt tgctgcggta cttgtatgca ccgttgacga
     2581 gttccatcag tcgggcgatg gctgtgttga acgagaacga ctcgaagtcg tcggttacga
     2641 ctttgatggt gcgatgtacg agtttcctca gttcggtatc ggccggagcg aaccccttct
     2701 cggccgtttg aacgagttcg ccggtctctt cgcataggcg ccacacgcgc tcgagccacg
     2761 ggaaagtgac ccggccgatg gcgaggacgc cctcctcggg ccaatcgaag ggggcttcgg
     2821 gggggccgga gaacagcatg tagagcctca gcgagtcggc gccgaaacgc tcgaaggctt
     2881 cctgcggctc gacgatgttg ccgcgcgact tactcatacg cttgccgccc atc
//
LOCUS       DATMED010000404         4306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5695718, whole genome shotgun sequence.
ACCESSION   DATMED010000404 DATMED010000000
VERSION     DATMED010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5695718"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1267)
                     /locus_tag="VNP73_10555"
     CDS             complement(92..1267)
                     /locus_tag="VNP73_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWL66403.1"
                     /translation="MTEVPELRGRAAIVGVADLASPTGELGMSGREVEVRMATEAIAD
                     AGLQMSDVDAVFASGNYGVSMSIAEHLGIQARYTDSTSVGGSSFEVHVEHAAAALALG
                     LCEVALIVYGATPRTTMKAGQKRDRGVPTSELLEWEIPFGLRMPIGGYALAASRHMAV
                     YGTTQEQLASIAVSTREWARMNPMARYQDPISTQDVLDSPFVCEPLHKLDCCLVTDGG
                     GALVMTSADRARDLPKPPAYVLGASTCHTHLMISAMPDLTVTAASVSGPKAFNMAGIK
                     PGDVDVVETYDSFTITVLLCVEDLGFCAKGEGGPFVEGNTLGPGGDLPLNTNGGGLSF
                     THPGMYGIFPLVEATRQVRGELAEGRQIDNAQIAVAHGSGGVLSSMSTIVLGSEETL"
     gene            complement(1264..2079)
                     /locus_tag="VNP73_10560"
     CDS             complement(1264..2079)
                     /locus_tag="VNP73_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWL66404.1"
                     /translation="MGRLDDRIAIVTGASRGIGAAIAKRIAEEGATVAVAARSEQQVD
                     ERLPGTIHTVVHEIEDAGGRALAVPTDMSKEEDRARLVETVARELGPPDILVNNAAVT
                     FFVPTTEFTRKRYDLMFEVQVWGAVDLARRVLPYMTEKKRGSILNISSKAAFHPQGPP
                     YDARFSLATVYGMCKAALERFSTGLASEVYEHGISVNALSPSKVVPTPGVLFHGLISA
                     DSPDAEPVEVMAEAALTLVSGDPNELTGRICFSQELLDEFGIEAPRSRAEATR"
     gene            complement(2081..2746)
                     /locus_tag="VNP73_10565"
     CDS             complement(2081..2746)
                     /locus_tag="VNP73_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWL66405.1"
                     /translation="MGGAWPAVPQIEDATPAKQRRAWGKVAHEDRFRDQRRQLLRAAA
                     KLASRKGYQATHVSDIVSEAGLSKTTFYEHFKSKEDCFVELHRRVSSAMLRAGISAAE
                     DAIEKGPFETVLAVIRAMTGYVAQDPRLADVLRDEVSASHSAIVQERKENQKHTIELF
                     ITLAQRFGSALPKEELEISSIVLVQGVIAVLPQLRKRAAGFDENLRSIARLACRGMGL
                     EAT"
     gene            2848..3396
                     /locus_tag="VNP73_10570"
     CDS             2848..3396
                     /locus_tag="VNP73_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66406.1"
                     /translation="MQRADRLYAIAGGLLHAVAVGIGACIGFVFTYIFLGPFAILGFL
                     LGGLPVWGLTRWMTMETDLSLAPPRKKGIALAYWVGVALAGAGVLIPVLSLPWLFGCT
                     DECQGFAVSIIFGGLVAFLGVLTLSSVASDVTVRISATSQVRVLTYDFRPAIRALWMF
                     LLADLVIVAFWWEPIAMHTSLF"
     gene            complement(3522..>4306)
                     /locus_tag="VNP73_10575"
     CDS             complement(3522..>4306)
                     /locus_tag="VNP73_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWL66407.1"
                     /translation="AEEYNTITLQLRDELAAAIDEGDADPESRVILLRAEGPAFCAGY
                     GLDWSTEAQAGESSGGRVWDSAADLRGISHFVDVYMKLWYAWKPTIAAVQGWCIAGGT
                     DMVLCADMIVAGEGASFGYPPSRVWGTPTTAMWVYRMGLERAKRYLLTGDEIPAPEAA
                     RIGLILEAVPDDQLQSHAMELAKRMALLPTNQLVMLKLLCNQTAENMGLASARTLGSL
                     FDGIARHTQEGLDFVHRAQDVGFRQAVRERDDPFGDYGSRPR"
BASE COUNT          731 a         1333 c         1345 g          897 t
ORIGIN      
        1 gtcctgcagt gctgaaggac aaactctcgg ttcttggtcg cgtcccagaa ggatgcggag
       61 atgtcggaga tcgggggctc gaagcgagta ctcataatgt ttcctcgcta ccgagcacga
      121 tcgtagacat cgacgagagc acgccaccgc tgccgtgtgc caccgcgatc tgagcgttgt
      181 ctatctgtct gccctcggcc aattcaccgc gcacctgtcg cgtcgcttcg acgagaggaa
      241 agatcccgta catgccggga tgcgtgaagg agaggccgcc gccgttcgtg ttgagcggta
      301 gatcgccgcc agggccgaga gtgttgcctt cgacgaaagg accaccttcg cctttcgcgc
      361 agaagccgag gtcttccaca cacaacagaa ctgtgatcgt gaacgagtcg tacgtctcga
      421 cgacgtcgac atcgcccggc tttatcccgg ccatgttgaa agcctttgga cccgatactg
      481 atgcagccgt caccgtgagg tccggcatcg cgctgatcat gaggtgtgtg tggcaggtgc
      541 tggcgcccaa gacgtaggcc ggtggcttcg gtaggtcacg cgcacggtcg gcgctggtca
      601 tcacgagcgc gccgccaccg tctgtcacga ggcagcagtc gagcttgtgc agcggttcgc
      661 aaacgaacgg tgaatcgagt acgtcctggg tcgagatcgg gtcctggtag cgagccatcg
      721 ggttcatccg agcccactcc cgtgtcgaca ccgctatcga tgcgagctgc tcctgcgttg
      781 tgccgtagac ggccatgtgc cggctcgccg ccagcgcgta gccgccgatc ggcatcctca
      841 gaccgaacgg gatctcccac tcgagaagtt cggaggtcgg gactccgcgg tcgcgcttct
      901 gcccggcttt catcgtcgtg cgaggcgtcg cgccgtaaac gatcaaggcg acttcacaaa
      961 ggccaagtgc cagcgcggca gccgcgtgtt cgacatggac ctcgaagctc gacccgccga
     1021 cggacgtcga atctgtgtag cgcgcctgga tgccgagatg ctcggcgatg ctcatcgaga
     1081 cgccgtagtt ccccgaagcg aataccgcgt cgacgtccga catctgaagg cctgcatcgg
     1141 cgatggcttc ggtggccatc cgcacctcga cctccctgcc gctcatcccc aactcgcctg
     1201 tgggggaggc gagatcggct acaccgacga tcgcggcccg ccctcggagc tctgggacct
     1261 cggtcatcta gttgcctccg ctctggatcg gggagcttcg atcccaaatt cgtccaggag
     1321 ctcttggctg aagcagatgc gtcccgtgag ttcgttcgga tcgccagaga cgagggttag
     1381 ggctgcttcg gccatgacct cgaccggctc ggcgtcggga ctatcggcgg agatcagtcc
     1441 gtggaagagg acgccgggag taggaacgac cttcgaggga gacagtgcgt tgaccgagat
     1501 cccgtgttcg tagacctccg acgcgagtcc cgtcgagaaa cgttccagcg cggccttgca
     1561 cattccgtaa acggttgcca gcgagaagcg cgcgtcgtac ggcggtcctt gggggtggaa
     1621 cgccgccttc gacgagatgt tgaggatcga cccccgcttc ttctcggtca tatagggaag
     1681 cacccggcga gcgagatcga ctgctcccca cacctggacc tcgaacatca ggtcgtagcg
     1741 cttgcgggtg aactcggtgg tgggcacgaa aaaagtcacg gccgcgttgt tgacgaggat
     1801 gtcggggggc ccgagctctc gcgcgaccgt ttcgacaagc cgagcgcggt cctcttcctt
     1861 ggacatgtct gtaggaacgg cgagggcacg tccgccggca tcttcgatct catgaacgac
     1921 ggtgtggatc gtgccgggga gccgttcatc gacctgttgt tcacttcgag ctgctacggc
     1981 gacagtcgcg ccctcttcgg cgatcctctt cgctatcgct gctccgatgc cacggctcgc
     2041 gccggtgacg atcgcgatcc ggtcgtcgag tcgtcccatg ctatgtggcc tccaatccca
     2101 taccgcggca cgcaagccgc gcgatcgatc tcaggttctc gtcgaagcct gccgctctct
     2161 tcctcagttg tgggagcacg gcgatcacgc cttgcaccag aacgatggag gagatctcga
     2221 gttcttcttt ggggagcgcg ctaccgaagc gttgagccag tgtgatgaac agttcgatcg
     2281 tgtgtttctg attctctttg cgttcttgaa cgatcgccga atgagatgcg ctgacctcgt
     2341 cacgaagaac gtctgcgagc cgggggtcct gcgcgacgta gccggtcatc gctcggataa
     2401 cggccaggac ggtttcgaat gggcctttct cgatggcgtc ctcggcggcc gagatcccgg
     2461 ctcgtaacat cgccgagctg actcgcctgt ggagctcgac gaagcagtcc tctttcgact
     2521 tgaagtgctc gtagaacgtg gtcttggaga gccccgcttc cgacacgatg tcggacacat
     2581 gggtggcttg gtagcccttc cgcgaggcca acttcgcggc ggcacgcagc agttgtctgc
     2641 gttggtctct gaaccgatcc tcgtgtgcga ccttgcccca agctcgccgc tgcttcgccg
     2701 gtgtggcgtc ttcgatctgg gggaccgcag gccatgcgcc gcccatctac agatgatagc
     2761 gattcagacg atttctgtcc tcatgggtat agctttgttg aagaccctta tgttccgggg
     2821 aggtcttcat ctggctagcc tggatggatg cagagagcgg accgtctcta cgcgatcgct
     2881 gggggtctcc tgcacgctgt tgcagtcggc ataggagcgt gcatcggatt cgtcttcacc
     2941 tacattttct taggtccttt tgccatcctg ggattcctct tgggaggact tccggtttgg
     3001 ggtctgacgc gatggatgac catggagacg gatctctcac tggcaccacc aagaaagaag
     3061 gggatcgctc tcgcgtactg ggtgggggta gcactagctg gggcgggcgt cctcatcccg
     3121 gttctatcgc tgccgtggct cttcggttgc accgatgagt gccagggctt cgccgtttcc
     3181 ataatctttg gcgggctcgt tgccttcctt ggtgtcctta cgctttcctc cgtggcgtca
     3241 gacgtgaccg tccggatttc ggctacgtcg caggttcgag tgttgacgta cgacttccgt
     3301 ccggcgatac gcgctctgtg gatgtttctt cttgcagatc tggtcatcgt cgcgttctgg
     3361 tgggaaccga tagccatgca cacgtcattg ttctagtggt ttcactggtt ctactggtcg
     3421 cgttgaaatc agtggaacca cttgaaccag tcaaaacgcc gggaccgccg agaaactatt
     3481 ggccgaggcg accgtcgatc cgctctcctc gatcatggca actagcgggg tcgcgacccg
     3541 tagtcgccga aggggtcgtc tcgttcgcgt acggcttgcc tgaagccgac atcttgcgcc
     3601 cggtgcacga agtcgaggcc ctcttgcgtg tggcgagcga tgccgtcgaa cagcgatccg
     3661 agggtgcggg ccgatgcgag gcccatgttc tctgcggtct ggttacacaa cagcttcagc
     3721 atcaccagct ggttcgtggg tagtagagcc atccgcttcg ccagttccat cgcatgcgat
     3781 tgcagctggt cgtccggcac ggcctcgagt atcagaccga tgcgagccgc ttcgggggcc
     3841 gggatctcgt cgccggtcaa caagtagcgc ttggcccgct cgaggcccat ccggtagacc
     3901 cacatcgccg tagtcggcgt gccccacacg cgcgacgggg gatagccgaa cgacgcgccc
     3961 tcgcctgcga cgatcatgtc ggcgcacagg accatgtcgg tccctccggc tatgcaccaa
     4021 ccttgaaccg ccgcgatcgt cggcttccac gcgtaccaga gcttcatgta cacgtcgacg
     4081 aagtgcgaga tgccgcgcag gtcggcggcc gaatcccaca cgcggccccc actcgactcg
     4141 ccggcttgcg cttcggtcga ccaatcgagg ccgtaaccgg cgcagaaggc cgggccctcg
     4201 gcccgcaaca ggatcacgcg actctcggga tcggcgtcgc cctcatctat ggccgcggcg
     4261 agctcatcac gtagctgcag ggtgatcgtg ttgtactcct ccgccc
//
LOCUS       DATMED010000405        10174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4929893, whole genome shotgun sequence.
ACCESSION   DATMED010000405 DATMED010000000
VERSION     DATMED010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4929893"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VNP73_10580"
     CDS             <1..473
                     /locus_tag="VNP73_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWL66408.1"
                     /translation="ITIMDGAGEHTGQVVLFDPKTDVAIVHVEGTEGPALPLLTKGLA
                     RGTPGATLGYPGGRQSLVAHRAAVQDRYDATGRDIYGRATVERDIYELRSPVRQGDSG
                     GPFVLPDGSVGGVVFAASTADDATGYALTGAEVRDEIEAGSNRSEAVSTGGCIR"
     gene            528..1661
                     /locus_tag="VNP73_10585"
     CDS             528..1661
                     /locus_tag="VNP73_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66409.1"
                     /translation="MSSDRVMAVACGVIALAVGAVAVMFSIAPHFWRVSALVRMDPRE
                     PMAEVARIADPDFAFIFDGHYDGVYAYAIALDPLARGHTHTLIDYPAYRYGRVGYGWL
                     AWAVTGGRARAIPTALLAIGLFSLGMAGYGASRLATQLGWSSSLGLLVGLNPGILLGL
                     TVDTSEPLTIALIVFALLAWLKDRTVTAVILMCFLALVKEPFLAVPAGLFLWELFMWR
                     KGYRAQDLFKRLALLTAPVIVVAAWWIYVRSVFGVWSFNQPWLVSPPIYGWLDGFKKT
                     AELATLGNVDQMQIGLAEVPLLLCVGVAFLMGFVRAIRFKTPLDPIFVFFAIIFSALS
                     WWQLVYPKDMIRILAIPLFFLPAVFPARELFIPSSRLPDRSQE"
     gene            1658..2428
                     /locus_tag="VNP73_10590"
     CDS             1658..2428
                     /locus_tag="VNP73_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWL66410.1"
                     /translation="MTLVPEAPRVSIVIPAHNEGSHIDILLDRLFESVTSECEVFVVY
                     DTVDDATAPFVDRYSQKEPRLKPLLNTYGPGPANAIRFGIDQATARVIVVTMADGSDD
                     PRQIDELTRLVERGVVVAAASRYAPGGQEVGAPLVKRLLSRIAGLTLHWFARVGTADA
                     TNSFKGYSAEFVRSVGIESKNGFSIGLELTAKARRLRLPVAELPTIWLERQTGVSNFN
                     VAGWIPLYLRWYFFAFGPRVTMDYFTERRVRSERESEA"
     gene            complement(2425..3474)
                     /locus_tag="VNP73_10595"
     CDS             complement(2425..3474)
                     /locus_tag="VNP73_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWL66411.1"
                     /translation="MTRSHPLRAGVIGLGYGAEVHLPVLMGMPDVEVVGIASRDAEKA
                     RATADAAGVSQAFGHSDELLGLDLDLVTLALPPMVNERVAEQALGRDLAVLSEKPIAS
                     TAEAARKLAELSIGKTTGVGFQFIELRTFQALKKVIGDEKLGRLRGVRVDWLTNSRAN
                     RDQSWSWKTDAGRNGGILTSMGAHVLFLVEWLFGRIGRVEASLDSSSSSRFAPAGDTA
                     AADSATLALHAEAAPVRVAISNASSGPLGHRWTMDFEEGTATLEEVRPIYLAGLRLTV
                     KGKGDAEVFEEAPESTDGRQAPFRALAERFVQATSVGAEVWPNFHDGARVQTLIDRVV
                     ESARRGEAVAVGSER"
     gene            complement(3471..4739)
                     /locus_tag="VNP73_10600"
     CDS             complement(3471..4739)
                     /locus_tag="VNP73_10600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66412.1"
                     /translation="MTDNRGTMGESARTIDGCQVCGRAGLESLLFLGYMRPGNMMDPI
                     GESPEPQELYPLEFLRCPACTLVQIGLEVDGRILFPPDFPYRTGNTRALVENFKQLFV
                     EVRSLIDLRDDDLIADIGSNDGTLLKNFAEAGYPVLGIEPSKAAQDAEAQGIPTEMRF
                     FDRDLVNELKDEGKRPRIITCTNTFAHMTEIVERVRALADLLPEEGVFVSESHYLLEL
                     VRSLQYDTIYHEHLRYYSVRSLIHLLEMADLEVFHVSRIPTHGGSIRVYAGHRGNRTP
                     HASVEETLEEEDGYGLTDGSALKTFRDRVVESKAGLWELLGGIKANGSTIYGIGAAVR
                     AATLIGYTGVDDSILDCILEVGSSDKIGKYMPGTRIPVLDEIKLFEAQPDYALLLSWH
                     LADYLATGLRERGFTGRFIAPLPQPRVLDL"
     gene            complement(4729..5721)
                     /locus_tag="VNP73_10605"
     CDS             complement(4729..5721)
                     /locus_tag="VNP73_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWL66413.1"
                     /translation="MSATRFLVTGGSGFIGAALVRRLVEGGHHVSVLDNNSRGDPRRL
                     KGVLDDIDFIPADIRDEKAVTSAAANVDVVVHLAFVNGTRFFYSAPELVLDVGVRGIL
                     NVLAACRAHDVDRLIVASSSEVYQSPSKIPTDESAPLVVPDVHNPRYSYGAAKLISEV
                     MAINYTRKDHGQTVIFRPHNIYGPDMGWDHVLPQLITRAVALVETTAQDPVPFPLQGD
                     GSQTRSFMFIDDCADALMTLIEEGEHLGIYHVGNPEERSIKSVAEKIFELLKREVEII
                     PGPTPAGETARRVPDTTRLNALGFKPEISFDQGLVPTFEWYANNRDLWPADDDG"
     gene            complement(5718..7088)
                     /locus_tag="VNP73_10610"
     CDS             complement(5718..7088)
                     /locus_tag="VNP73_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWL66414.1"
                     /translation="MVTTRPSRTPGDRTVCIMGLGYVGLTLATTMAEVGFDVYGIERD
                     EALADRLQSGDPHFFEPGMAERLNQALHDKQLKISTTIPAECPATVYIVAVGTPLGPD
                     QRVRTDIVETVAAEIAGRMKDGDMVVVRSTVKIGTTRGIILPILESSGKDFDLAFCPE
                     RTQEARALEELRQLPQIVAGYTKRANDRAAQLFQAVTPTIIRVSDLETGEVIKMVDNA
                     HRDVYFAFANEVARICDAVGISALEVIQSGKLGYPRTNLPLPGTVGGPCLTKDPYILA
                     ESLEEFGVEPEITLAARHLNERLPKEAVAHIAGVCSSVGDFPSDPTIALMGLAFKGRP
                     PTDDVRGTTARLVLDELREAFPQGRFRGFDAMVSDAVIQGFALEPHSSIESAMTDANL
                     VVILNNHPDFAAMPLEEHSDLMARPGLIYDFWNQFTDVHLRLADSVSYIALGSHGRRI
                     SGPAGR"
     gene            7109..7888
                     /locus_tag="VNP73_10615"
     CDS             7109..7888
                     /locus_tag="VNP73_10615"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HWL66415.1"
                     /translation="MQVDQIRQALIAAGVTGPHASHSREGNIRKIHTLLNGEEPEATF
                     GLSGLDRYSASEVLGFMSDLTGCPVDIADLSCADGIDPDRTIRGLIAAGEKLRDEAKK
                     GSSMLAATGHPTGMLEFYMRLSDAFVRAGGKEVRLREGEDLPIGRSGRTREVRYVGGV
                     GCVADWGSLKHTHSSAAMEALLESEPWPALVLGDHGFAGAALERGIPTIAIMDINDHA
                     LAIAHAERKDVTIVPLDDNRPPRLYEPALALIESVIFETDE"
     gene            7881..8834
                     /locus_tag="VNP73_10620"
     CDS             7881..8834
                     /locus_tag="VNP73_10620"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWL66416.1"
                     /translation="MSKELEQAVRTVLVDCLAVESGENVLVVTDPPKVALARALVDGA
                     RSMGAEVVVAEMSERVTQGTEPPPQIAAAMLAADVVIAPTTKSLSHTEARHAASEKGA
                     RIATMPGVTEEMLGRTMSADFKAIAERSAAIASLLSEGSVVHITTEKGTDVTFDIRGR
                     EGLADDGGIQAPGAFGNLPAGEAFVAPVERKTNGRIAFDGSIWPVGVLEDPLVIDIED
                     GYARGFSGSAAEEFRSKLEPWGDEAFAVAELGIGTNEAAQLTGNVLEDEKILGTIHVA
                     FGDNHSFGGKIRVASHQDGIVLKPTVVIDESPLLTAGRMLI"
     gene            8878..9093
                     /locus_tag="VNP73_10625"
     CDS             8878..9093
                     /locus_tag="VNP73_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWL66417.1"
                     /translation="MSARFDPSADTSLGGAFLTVGEVARHLRVSNMTVYRLVKSGQLS
                     AVRIGRGYRIREADMHKYLEQRYMDAG"
     gene            9183..10073
                     /locus_tag="VNP73_10630"
     CDS             9183..10073
                     /locus_tag="VNP73_10630"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HWL66418.1"
                     /translation="MKPEIEAAYERLIGSPGTVVLLGGIDAGKTSFGIGLLRRANEAG
                     VPAAFVDADIDQSSVGPPTTIGLRLTTGLQEIDHDTLREADSLAFVGSLTPREHLLPI
                     VSGTGKLIRKARQAGCRLVVVDTTSFVSGIYGQTLNYFLMDSADPDFVVAFERGGELE
                     PLVGIAHRFTPAEVIELHVQTDAVRSAEEKVSFRERQFAAYFEHTSRWRVKPTVFMPT
                     LPPGFDLHLLDGLVVGMEDGKGACVGIGVLEHDPEEDILRMVSPVTEGVKGLRLGSVR
                     MHIGGHSRGPVHLRDLFGTE"
     gene            10092..>10174
                     /locus_tag="VNP73_10635"
     CDS             10092..>10174
                     /locus_tag="VNP73_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HWL66419.1"
                     /translation="MPSLVKKRRKKMRKKKHRKMLKKTRWQR"
BASE COUNT         1851 a         3060 c         3187 g         2076 t
ORIGIN      
        1 cgatcacgat catggacggc gccggcgagc acaccgggca agtcgttctc ttcgatccca
       61 agaccgatgt tgcgatcgtt cacgtcgagg gcacggaggg tcctgcgctc cctctcctga
      121 cgaaagggct ggcccgcggc acgccgggcg caaccctcgg ttacccgggc gggcggcagt
      181 cgctcgtagc tcaccgagcc gccgttcagg atcgctacga cgccacggga cgtgacatct
      241 acggccgagc aaccgtcgaa cgtgacatct acgaattgcg ctcgcccgtg cgccagggag
      301 actcgggtgg cccgttcgtg ttgccggacg gttcggtggg tggggtcgtg ttcgcggcgt
      361 ccaccgccga cgacgcgact gggtatgcgc ttacgggggc cgaggtgcgg gacgagatcg
      421 aggccgggag caaccgctcg gaggcggttt cgactggggg gtgcatccgt taagtacggc
      481 tcggttagcc ggttgataaa gagccgtaac ccctatgctc ttgccccatg agttctgacc
      541 gcgtgatggc ggttgcctgc ggcgtgatcg cattggccgt cggcgcagtg gcggtgatgt
      601 tctccatcgc cccccacttc tggcgggtta gtgcactcgt tcggatggat ccccgcgaac
      661 ccatggccga ggtggctcgg atcgccgacc ccgacttcgc tttcatcttc gatgggcatt
      721 acgacggtgt ctacgcctat gcgatagcgc tggatccact ggcacgaggt cacacccaca
      781 cgctgatcga ctatccggcc tataggtacg gtcgcgttgg ttatgggtgg ttggcatggg
      841 ccgtgacggg cggtcgtgcc agagcgatcc cgacggcgtt gctcgcgatc ggcttattca
      901 gcttgggtat ggcggggtac ggcgcgagca ggttggcgac gcaattggga tggtcgtcat
      961 ctcttggcct tctggtcgga ttgaaccccg ggatcctcct cggcctgacc gtcgacacca
     1021 gcgaacccct cacgatcgcc ctgatcgttt tcgccttgct ggcgtggctc aaggaccgca
     1081 ccgtgaccgc ggtgatcctg atgtgtttcc tggccctcgt gaaggaaccc ttcttggccg
     1141 tccccgcggg gctgttccta tgggagttgt tcatgtggag gaagggctat cgggcgcaag
     1201 atctattcaa gaggcttgcg ttgctcacgg caccggtcat cgtcgtcgcc gcatggtgga
     1261 tctacgttcg gagtgtcttc ggtgtctggt ccttcaacca gccgtggctc gtgtcgcctc
     1321 cgatctatgg atggttggac ggattcaaga agacggccga actggcaacg ctcggcaatg
     1381 tcgaccagat gcagatcggt cttgccgagg tgcctctgct gctatgcgtc ggcgttgcct
     1441 tcttgatggg attcgtccgg gcgatccgat tcaagacccc cttggacccg atctttgtct
     1501 tcttcgcgat catcttcagc gcgctctcgt ggtggcaact cgtctacccc aaggacatga
     1561 tccgcatcct cgcgatccca ctgttcttcc tcccagctgt tttcccagcc cgcgaactgt
     1621 tcatcccgag ttcccggctg ccggaccgtt cacaggaatg actctcgtgc ccgaggcgcc
     1681 gcgtgtgtcg atcgtgatcc cagcccacaa cgagggatct cacatcgaca tccttctcga
     1741 ccgattgttc gaaagcgtga cttcggaatg cgaagtgttc gtggtctacg acaccgtcga
     1801 tgacgcgacg gctccgttcg ttgatcgata ttcgcagaaa gagccccgcc tcaaaccgtt
     1861 gctgaatacg tacgggccgg gacccgcgaa cgccatccgg ttcggcatcg atcaggcgac
     1921 cgctcgcgtg atcgtcgtca cgatggcaga tgggagcgac gatcctcgtc agatagatga
     1981 actgacacgt ctggtcgaga gaggcgtcgt tgtggcggct gcatcccggt atgccccggg
     2041 tggtcaagaa gtaggcgcgc ctctggtcaa aagactgttg tctcgcatcg cgggtttaac
     2101 gctgcattgg ttcgcgcgcg tcggcaccgc cgacgcgacg aattcattca aggggtattc
     2161 cgccgaattc gtccgttcgg tggggatcga gagcaagaac gggttctcta tcggtctcga
     2221 gctcaccgca aaagcgcggc ggttgcggtt acccgttgcc gagttgccca cgatctggct
     2281 ggagcggcag accggcgtat cgaatttcaa cgtggcaggt tggatcccgc tgtacctccg
     2341 ctggtatttc ttcgcctttg gtcctcgtgt gacgatggac tacttcacgg aacgccgcgt
     2401 tcgatcggag cgcgagagcg aggcttaacg ttcggatccg accgcgaccg cctcgccgcg
     2461 ccgcgctgat tcgacgaccc gatcgatcaa cgtctgtacc cgggcaccgt cgtggaagtt
     2521 gggccacact tcggccccca ccgaagtggc ctgcacgaac ctctccgcca gggctctgaa
     2581 tggagcctgt cgaccgtctg tcgactccgg tgcttcctcg aacacctctg cgtcgccctt
     2641 tcccttcacc gtcaaccgca accctgcgag atagatcggt ctgacctctt ctaacgtcgc
     2701 cgtgccttct tcgaagtcca tcgtccatct gtgtccgagg ggcccggacg aggcgttgct
     2761 gatggcaact cgaacggggg cagcttcggc gtggagtgcg agcgttgcag agtcggccgc
     2821 cgccgtatcg cccgcaggcg cgaaacggct actcgaagag gaatcgagcg aggcctcgac
     2881 gcggccgatc cgaccgaaca gccattcgac gaggaagaga acgtgagcac ccatggacgt
     2941 gaggatccct ccgtttcgac ctgcatcggt cttccacgac cagctttggt ccctattggc
     3001 tcgcgagttc gtcagccagt cgacccgaac acctcggagc ctgccgagct tttcatcccc
     3061 gatgaccttc ttcagagctt ggaacgttcg caactcgatg aattggaatc caactcctgt
     3121 cgtctttccg atgctcagtt ctgcgagctt gcgggcggct tcggcggtgg aggcgatcgg
     3181 cttctcggag agaacggcga ggtctcggcc gagtgcctgt tcggccacgc gctcgttcac
     3241 catgggtgga agagcgagcg tcactagatc tagatcgagt cccaacagtt catcgctatg
     3301 cccgaatgct tgcgatacac ccgcagcgtc tgctgtggcg cgggccttct ccgcatccct
     3361 gcttgcgatg ccgacaactt ctacgtccgg catgcccatc aacacgggta gatgcacctc
     3421 ggctccgtag ccgagtccga taacccctgc tctgagcgga tgcgaacgcg tcaaagatcg
     3481 agtactcgag gctgcgggag aggcgcgatg aatcttccgg tgaaaccccg ctcccgaaga
     3541 cctgttgcga gatagtcggc gaggtgccac gacaggagca atgcgtagtc gggctgtgcc
     3601 tcgaagagtt tgatctcgtc cagaaccggg atgcgggtcc caggcatgta cttacctatc
     3661 ttgtccgagg agccgacctc gaggatgcag tcgaggatcg agtcgtccac ccccgtgtag
     3721 ccgatcagag ttgcagctcg cacggcagca ccgatcccgt agatcgtgga accgtttgct
     3781 ttgatgccac ccagcagctc ccacaatcct gccttcgatt cgacgacccg atcgcggaag
     3841 gtcttcagtg cgctcccgtc tgtgagtccg taaccatctt cttcttccaa cgtttcctcc
     3901 accgaggcgt gcggtgtcct gttgccccga tgaccggcgt agacccggat cgatccgccg
     3961 tgtgtcggta tccgtgagac gtgaaacacc tcgaggtccg ccatctcgag gaggtggatc
     4021 aacgaacgga ccgagtagta gcgaaggtgc tcgtgataga tggtgtcgta ttgcagcgac
     4081 cggacgagtt ccaagaggta atgggattcg ctgacgaaca caccctcctc ggggagcaga
     4141 tctgcaagcg ctcgcactcg ttccacgatc tccgtcatgt gcgcgaacgt gttcgtacaa
     4201 gtgatgattc gtggacgttt cccttcatcc ttgagttcgt tcaccaggtc ccgatcgaag
     4261 aaacgcatct ctgtagggat cccttgagct tctgcgtcct gcgccgcttt ggatggctcg
     4321 atcccgagca ccggatatcc cgcttcggca aagttcttca atagcgttcc gtcgttcgag
     4381 ccgatgtctg cgatgagatc gtcgtccctg aggtcgatca aggaccggac ttcgacgaaa
     4441 agctgcttga agttctccac cagggcccgc gtgttccccg ttctgtaggg aaagtccggt
     4501 gggaacagga tccggccgtc cacctccaaa ccgatctgga cgagagtgca tgcggggcac
     4561 cggaggaact cgagtggata aagctcctgg ggctctgggc tctctccgat cgggtccatc
     4621 atgttgcccg gtcgcatgta accgaggaaa agtagggatt ccaacccggc acgaccgcag
     4681 acctggcagc cgtcgatcgt cctcgccgac tcgcccatcg ttcctctgtt atccgtcatc
     4741 gtcggccggc cacaggtccc tgttgttggc gtaccactcg aaggtcggta ccagtccttg
     4801 atcgaatgag atctccggtt tgaagccgag cgcattcaat cgtgtggtat cgggaacgcg
     4861 gcgggctgtt tctcctgcag gtgtgggacc ggggattatc tcgacctctc ttttaagaag
     4921 ctcgaagatc ttctcggcca cgctcttgat cgaccgttct tccgggtttc cgacgtggta
     4981 gatgcccaag tgttccccct cttcgatcaa ggtcatgagc gcgtcggcac agtcgtctat
     5041 aaacataaag gagcgtgtct gtgagccgtc accctgcagc ggaaatggca ctggatcctg
     5101 cgcggtggtc tcaacgaggg caaccgccct tgttatcaat tggggcagaa cgtgatccca
     5161 acccatgtcc gggccgtaga tgttgtgtgg tcgaaagatg accgtctgcc catgatcctt
     5221 gcgcgtgtaa ttgatggcca tgacttcgct gatcagcttt gccgcgccgt agctataacg
     5281 agggttgtga acgtcgggaa cgaccaaggg tgcggattcg tccgtgggga tcttggacgg
     5341 cgattggtac acctcggaac tcgacgccac aatcagacga tcgacatcgt gcgccctgca
     5401 ggccgcgaga acgttaagga tgccgcggac tccgacgtct aggacgagtt cgggcgccga
     5461 gtagaagaat ctcgttccgt tcacaaaagc gaggtgaacg acgacgtcta cgttcgctgc
     5521 tgcacttgtc acggcctttt catcgcggat atcggccggg atgaagtcga tgtcgtccaa
     5581 cacgcccttc aatcgcctcg ggtcgccccg ggagttgttg tcgagcacgc tgacgtggtg
     5641 tccaccctcg acgagacgtc gcaccagcgc ggccccgatg aatcccgacc cgccggttac
     5701 gaggaaacgg gttgcgctca tcgcccggcc gggccggaga tccgccggcc gtggcttccc
     5761 agcgcgatat agctgaccga atccgcaaga cgaaggtgta cgtcggtgaa ttggttccag
     5821 aagtcgtaga tcagtcccgg ccgggccatg agatcggaat gttcttcgag cggcatggcc
     5881 gcaaagtccg gatggttgtt gaggatcacg acgaggttcg catcggtcat cgccgactcg
     5941 atcgagctgt gtggctcgag cgcgaagcct tggatcaccg catccgacac catcgcatcg
     6001 aaccctctga acctcccctg gggaaaggcc tcccgcagct cgtctaggac cagccgggcc
     6061 gtcgtgccgc gcacgtcgtc ggtcggagga cgacccttga aggcaagacc catcaaggcg
     6121 atcgtcggat cggacggaaa gtcacccacg gaagagcaga ctccggcgat gtgagcaacc
     6181 gcctctttgg gaaggcgctc gttcaagtga cgggcggcga gcgtgatctc gggctccact
     6241 ccgaactctt cgaggctctc ggcaaggatg taagggtcct tggtcagaca gggccccccc
     6301 accgttcccg gcagcgggag gttggttcgg ggataaccga gtttgccgga ttggatcacc
     6361 tccagggcgc tgatgcctac ggcgtcgcag atccgtgcca cttcattggc gaatgcgaag
     6421 tagacgtctc gatgagcgtt gtccaccatc tttatgacct cgcccgtttc cagatcgctg
     6481 acccggataa tcgtcggcgt aacggcctga aagagctggg ccgcgcgatc attcgctcgt
     6541 ttcgtatacc cggcgactat ctgcggtagt tgacgcaact cttccagggc cctcgcttcc
     6601 tgcgttcgct cggggcagaa ggcaaggtcg aaatccttcc cggaggattc gaggatgggc
     6661 aagatgatcc ctcgagtggt tccgatcttg acggtggatc tgaccacgac catgtcgccg
     6721 tccttcatgc gtcccgctat ctcggctgcg actgtttcga cgatgtcggt tcgaacccgc
     6781 tgatccggac cgagtggtgt gcctacggcg acgatgtaaa cggtcgccgg acactcggcg
     6841 gggatcgtgg tgctgatctt cagttgcttg tcgtggaggg cttgattgag ccgttcggcc
     6901 atgcccggct cgaagaaatg tgggtcgcca ctctggagcc gatcggccag ggcctcatcg
     6961 cgctcgatcc catagacgtc gaaccccact tccgccatcg tcgtcgcaag ggtcagtccc
     7021 acatagccca agcccatgat gcagacggtt cggtctccgg gcgttcgaga gggtcttgta
     7081 gtcaccattg ccgaactact ctacgggcgt gcaggtagac cagatccgac aagctttgat
     7141 cgcagcgggg gtcaccggac cgcatgcctc tcacagcagg gagggcaata tccggaagat
     7201 ccacacgctg ctgaacggtg aggagcccga agcgacgttc gggctgtcgg gcctcgaccg
     7261 ctactcggcc tccgaagtgc tcggcttcat gtccgatctc accggttgcc cggtcgacat
     7321 cgccgatctg tcttgcgccg acgggatcga ccccgatcgc acgattcggg gactgatcgc
     7381 ggccggcgag aagcttcgtg acgaagcgaa gaagggcagt tcgatgctcg cggcgacggg
     7441 gcacccgacc gggatgctcg agttctacat gcggctgtcg gacgcgttcg tccgggcggg
     7501 aggcaaggaa gtgaggcttc gcgagggtga ggaccttccg atcgggaggt cggggcgtac
     7561 gcgcgaggtt cgctacgtcg gtggcgtggg atgcgtggcg gattgggggt ccctcaagca
     7621 cacgcattcg tcggcagcga tggaggctct actcgaaagc gagccgtggc ccgctctcgt
     7681 cctaggcgat catgggttcg ccggtgcggc gctcgaacgt ggcatcccta cgatcgcgat
     7741 catggacatc aacgaccacg cgctcgcgat cgctcacgcc gagaggaagg acgtcacgat
     7801 cgttccgttg gatgacaacc gtcccccccg gttgtacgaa cccgctctgg cgttgatcga
     7861 atcggtcatc ttcgagaccg atgagtaaag agctcgagca ggcggtccgc accgtcctcg
     7921 ttgattgctt ggcggtggag agtggcgaga acgtcctggt cgtgaccgac ccgcccaagg
     7981 ttgcgcttgc ccgggcactt gtcgacggag ctcgctcgat gggggccgaa gtcgtcgtgg
     8041 ccgagatgag cgagcgagtt acccaaggta ccgagccacc gccacagatc gccgccgcga
     8101 tgctcgccgc ggacgtcgtg atcgcaccca ccaccaaatc gctatctcac accgaagctc
     8161 gtcacgccgc gagcgagaaa ggcgcacgca tcgcgaccat gccgggcgtg acggaagaga
     8221 tgctcggacg cacgatgagc gcagacttca aggcgatcgc cgaacgctcg gccgccattg
     8281 catctcttct ttctgagggt tcggtggttc acatcactac cgagaaggga accgacgtca
     8341 cgttcgacat ccgaggtcgt gagggattgg cagacgacgg agggatccag gcacccggtg
     8401 cgttcggaaa cctcccggcc ggtgaagctt tcgtcgcgcc ggtcgaacgc aagacgaacg
     8461 gcaggattgc gttcgatggg tcgatctggc ccgtaggcgt cctcgaggat cccctcgtca
     8521 tcgatatcga ggacggctac gcacgagggt tctcaggctc ggcggccgag gagttccgct
     8581 ccaagctcga accctggggt gatgaggcgt tcgcggtcgc ggagctgggg atcggaacga
     8641 acgaggcagc gcaactcacc gggaacgtcc tggaggacga gaagatcctg ggcacgatcc
     8701 atgtggcttt cggcgacaac cattcgttcg gcggaaagat ccgggtggct tcacatcagg
     8761 acggcatagt cctcaagccg acggtggtga tcgacgagtc gcctctgctc acggcagggc
     8821 ggatgttgat ctaggggctg ttgggccaag gggggccttg ctatactgct ggttcgcatg
     8881 agcgcgcgct tcgatccgtc cgcggacaca tcactgggag gagcattcct cacggtcggc
     8941 gaggttgcgc gtcaccttcg ggtatcgaac atgacggttt atcgacttgt taagtccggt
     9001 caactctcag cagtcaggat cgggcggggt taccgcatcc gcgaggccga catgcacaag
     9061 tacctggagc agcggtacat ggacgcgggc tagacggcac ctatctagaa caggctgttc
     9121 gcccgcgaga ccgtccacga ggacggtctt ttgatttatg gattgttggg ggtgctgacg
     9181 tcatgaagcc cgagatcgaa gctgcttatg agcggctgat cggaagtccg ggaaccgttg
     9241 ttctgctcgg cgggatcgac gcgggcaaga catcgttcgg tatcggcttg cttcgacgag
     9301 ccaacgaggc gggtgtcccg gccgcgttcg tagacgcgga tatcgatcaa tcctcggttg
     9361 gtccgcccac cacgatcgga ctgcggctca cgaccggact acaggagatc gaccacgaca
     9421 cactgcggga ggccgattca ctcgcgttcg tgggctcgct tactccgcgt gagcacctgc
     9481 tgcccatcgt ctccggtacc ggcaagttga tcagaaaagc gcgccaggcc ggatgccggc
     9541 tcgtggtcgt agacaccacg agtttcgtgt caggtatcta cggccagacg cttaactact
     9601 tcttgatgga ctcggccgac ccggacttcg tggttgcgtt cgagcgaggc ggcgaattgg
     9661 aacctctggt cgggattgcc caccggttca ctccggcgga agtgatcgag ctccacgtcc
     9721 agaccgatgc cgttcgctcg gcggaagaga aggtctcctt ccgggagcgt cagttcgccg
     9781 cctacttcga acacacctcc cgctggcgtg tgaagcccac cgtgttcatg cccacgctgc
     9841 cccccggatt cgacctgcat ctcctcgatg gtctcgtcgt ggggatggag gacggcaagg
     9901 gggcctgtgt tgggatcgga gtgctcgagc acgaccccga agaggacata ttgcgtatgg
     9961 tttcacccgt taccgaaggg gttaaagggc tgcggctcgg gtccgtcagg atgcacatcg
    10021 gcggtcattc acggggtccg gtgcacctgc gtgatctatt cggcaccgaa taagagagga
    10081 tgtaaggcaa catgccgtca ctcgtaaaga aacgtcgcaa gaagatgcgc aagaagaagc
    10141 atcgcaagat gctgaagaag acgcgctggc agcg
//
LOCUS       DATMED010000406         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1858797, whole genome shotgun sequence.
ACCESSION   DATMED010000406 DATMED010000000
VERSION     DATMED010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858797"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VNP73_10640"
     CDS             <1..383
                     /locus_tag="VNP73_10640"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWL66420.1"
                     /translation="DTNGLEIDKTNTWLYAVTTFSFNAEVYRVLLEDPSVVEKVADLG
                     SVTDPANGLDDMTFGKDGNLYITANGMGRVWRLDPETGDRCIIASGLQNPTAAKFGSG
                     PGWSRENLYVTSWDGTLRELTPPE"
     gene            complement(400..828)
                     /locus_tag="VNP73_10645"
     CDS             complement(400..828)
                     /locus_tag="VNP73_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66421.1"
                     /translation="MGLVWAVAILASLLLLIHLSTSGRNLVDVTVSDEEVTVTPKGLN
                     KLWAFKGQISVPISSIRSVRLVTGLRDLPMGIRWPGTAIPGLIVAGTYRKGGERSFYA
                     IRDGRDVVLIELDGHEYRRVGFQTNGPQEVVDRISSARNG"
     gene            1028..1831
                     /locus_tag="VNP73_10650"
     CDS             1028..1831
                     /locus_tag="VNP73_10650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HWL66422.1"
                     /translation="MRTRSILVLLCGSLLAAALAFPAQAITDGTRDGDGHPMVGALLK
                     TPLRDGSPGYYATCTGTLIDEDTFLTAAHCFFGSTDVLVSFDDQIESNENEQDDTYAG
                     EFIAHPQYQTATKYDLAVVELDEAVDITPAELPEEGFLDEQTLVSKTTPFTNVGFGTD
                     RPSKGQGGHMLFQSGYRQISNSTFKGLNSNSLSLWQKAKKGDGGACHGDSGGPTFWGS
                     SDDHEDDDHEDLIVGVIVTADTWCQSTLVDARLDTDAARDFLDTYVDLP"
     gene            1887..>2732
                     /locus_tag="VNP73_10655"
     CDS             1887..>2732
                     /locus_tag="VNP73_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWL66423.1"
                     /translation="MPRSVDNVGPDVLIERAENRTIRLDEGADTFLIGRDAERALIGK
                     AFSASVSQSRCHGVLLLAEPGLGKSLLASDTIARLGTYAETALTVCQEWAEGSLAPLE
                     RLVQAVAPGVKDDPEAALKDLVPIDEERVAIAEQLCRLFGWGDAPEVPDVSSWSVRRL
                     LENVSTQTPLVLVFEDLHSAEDPLLDTLEDLMIRLVGPITFLLTGHPDVVVRRPTLGL
                     PRENFHVRRLEALGRETALKLAEDVLGGQIVRGPWLENLLATANGNPLFLVEALVQLR
                     DDGHLQ"
BASE COUNT          543 a          831 c          837 g          521 t
ORIGIN      
        1 ccgacacgaa cgggctagag atcgataaga cgaacacgtg gttgtacgcg gtgaccactt
       61 ttagcttcaa cgccgaggtc tatcgcgttc tgctggaaga cccttcggtc gtcgagaaag
      121 ttgcggatct cggttccgtt accgatccgg ccaatggtct cgacgacatg acgttcggca
      181 aggacgggaa cctgtacatc accgcgaacg gcatgggccg agtctggcga ctcgatcccg
      241 aaacggggga tcggtgcatc atcgcttcgg gtctgcagaa cccgaccgcg gcgaagttcg
      301 gaagcggccc cgggtggtca cgcgagaacc tctacgtaac gagctgggac ggaacgctga
      361 gggagctaac gccgcccgag tagttgtccg gatagacgtc tagccgttcc tcgcggagct
      421 gatccggtcc accacttcct gtggaccgtt cgtttggaaa ccgacgcgtc ggtattcatg
      481 gccgtccagc tcgataagta cgacgtcccg gccatccctg atcgcataga acgatctctc
      541 cccacccttc cggtacgtgc ccgcgacgat cagaccgggg atcgcggtgc ccggccacct
      601 gatccccatg gggagatcgc gcagtcccgt gaccagtcga acgcttcgga tcgaggagat
      661 ggggaccgag atctggccct tgaaggccca cagtttgttg aggcccttgg gcgtgaccgt
      721 tacttcctcg tcggagacgg tcacatcgac gagattccga ccggaggtgg agagatgtat
      781 cagcagaagg agcgatgcga ggatcgccac cgcccacacg aggcccatgc ggccaaagtt
      841 attccctgag cctccgctct ctgcttagaa cgattcaaac aggctcgatc gtcgaagctc
      901 gtaaagatcg aagtcctgct gcacttacta cgcattcaac gcattccact tgtttccacc
      961 cgaacattcc cataacgtcc agccctaggc aatggggcag attacttggc gatcaagagg
     1021 gggcaccatg agaacgcgtt cgatacttgt cttgctgtgc ggttctttac tggcggcagc
     1081 gctggccttt cccgcacagg ccatcaccga cggcacgagg gacggcgacg gccacccgat
     1141 ggtcggcgct ctcctgaaga cgccgttgag ggacggatcg cccggctact acgcaacctg
     1201 caccgggacc ctcatcgatg aggacacttt cctcactgcg gcccactgtt tcttcgggtc
     1261 cacggacgtc ctcgttagct tcgacgacca gatcgagtcg aacgagaacg agcaagacga
     1321 cacgtacgcc ggagaattca ttgcccaccc gcagtaccag accgcgacca agtacgacct
     1381 cgcggtcgtg gagctggacg aggcggtcga tatcacgccg gcagagctcc cggaggaagg
     1441 cttcctcgac gagcagacac tcgtttcgaa gacgactcct ttcacgaacg tcggcttcgg
     1501 caccgatcgc ccgagcaagg gtcaaggcgg ccacatgctg ttccagagcg gctaccgtca
     1561 gatctcgaac agcacgttca aaggcctcaa ttccaactcg ctctctctat ggcagaaggc
     1621 gaagaagggc gatggcggcg cctgccacgg cgactccggc ggcccgacgt tctgggggag
     1681 ttcggacgac cacgaggacg acgatcacga agacctgatc gtgggagtga tcgtcactgc
     1741 ggacacctgg tgccagtcga cgttggtcga cgctcggctc gacaccgacg cagctcggga
     1801 cttcctcgac acgtacgtcg accttccata gctgttaacg gagccctgcc gggccatcgg
     1861 gggttggcga aagtaagggg gttggagtgc cacgatctgt ggacaacgtc gggccggatg
     1921 tcttaatcga gcgggccgaa aaccgaacga tcaggcttga cgagggcgcg gacacctttc
     1981 ttatagggcg cgacgccgag cgtgctctga tcggcaaagc gttctccgcc agtgtgtcgc
     2041 agtcgagatg ccacggtgtg ctgctcttag cggaacccgg tttggggaaa tcgcttctcg
     2101 catccgacac gatcgcgcgt ctcggaacgt atgcggagac ggcgctcacc gtttgccagg
     2161 aatgggccga gggcagcttg gccccactcg agagattggt gcaggcagtc gcacccggtg
     2221 tcaaagacga tcctgaggcc gccttgaagg acctcgttcc gattgacgaa gagcgggtcg
     2281 cgatagccga gcaactatgc agattgttcg gttggggtga cgctcccgag gttcccgatg
     2341 tttcttcttg gagcgtccgc cggttgctcg agaacgtatc cactcagacc ccgctcgtct
     2401 tggtcttcga ggatctgcat tcggccgagg atcccctcct tgacacattg gaagacctga
     2461 tgatccggtt ggtggggccg atcacattct tgttaacggg gcacccggat gtagtggttc
     2521 gtcggccgac actcgggctg ccgcgcgaaa acttccacgt ccggcgcctt gaggccctcg
     2581 gccgtgagac agctctcaag ctggccgagg acgtattggg tggtcagatc gttcgagggc
     2641 cgtggctcga gaacctgctc gccacagcca acggcaaccc cttgttcctg gtcgaggcgc
     2701 tcgtccaact gcgcgatgac ggacatcttc ag
//
LOCUS       DATMED010000407         3275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_333980, whole genome shotgun sequence.
ACCESSION   DATMED010000407 DATMED010000000
VERSION     DATMED010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333980"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VNP73_10660"
     CDS             complement(<1..630)
                     /locus_tag="VNP73_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66424.1"
                     /translation="MNRPRLLQRRVDIWKDPWTKIEESDPPYSAFILSLTRASVGLLA
                     LAAGLMVIGNLQDNGRLIWGALALMVPAVLYLLLVYAWAWARAALDEVSYGNITLDES
                     EPPPTVTAQKPSRNRIEAWWRARRRKKPTERRSDANMVWRLTKECRRYWIHIVGVLLI
                     SLAATPIFLLSPIPLKIAVDSVLKGLPLPEILSDHLPGFFSSSGFSLLVV"
     gene            complement(623..3130)
                     /locus_tag="VNP73_10665"
     CDS             complement(623..3130)
                     /locus_tag="VNP73_10665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL66425.1"
                     /translation="MTTDTGATPHIEGLARESVGSQGSISPTPFVSNGHPQLHQKVRA
                     DGNHLLLGGDPFRVKGVTYGTFEARADGALFPESGKLKNDLIAMKEHGLNTVRTYTLP
                     PVELLDVAAELDLKVIVGVDYLDWRYETQPGGAANRRVRDTGLRAVERALELCGGRPE
                     VLALSVGNEVPVDVARTHGISTVERVLAELIDAVHSGDPEMLATYCNFPTTEFLQISG
                     QDIVCLNVFLEDPRTFRRYIRHLQVVADDRPLILTELGLASAIHGEEEQAAALSWQLR
                     VLDECGVAGATVFAWTDEWAVNNKAVEGWGFGVTDTERRPKQSLEVLRRWTSLSIADL
                     RNHWPRVSVVICAYNAGEHIEQCLRSVANCSYPDLEVIVCDDGSTDDTLERARRFPFQ
                     VLELGHGGLSAARNAGIAAATGQIVAFLDSDAFCHPGWPFHLALSLENDSLAGTGGPN
                     LPVENAGLVERAVAASPGGPIHVLVTDDRAEHVPGCNMAFRKSDLVAVEGFDPAYTTA
                     GDDVDVCWKLLDAGRDISFSAAAQVRHHRRSSVKGYLKQQRGYGRAERMVAAKHPHRF
                     NRLGQARWSGFIYGGPRILATLLRPLIYHGYQGMAPYQGVLHRRAEVARNLATAFLPL
                     FAAFAVCGALAAPFTSVGRWIGVASMILTLAYWIAVTVAAHPTRTEPHPLRYRTLVGF
                     FYLSQPIVRAWGRVFYRSSVEKRGKPPAPTLWTGERIHWLRRIENGLREKDLSVRIGG
                     PHDHWDIETSFGLTVAQRLSVAVVWGWTPVSRSRMRLRAPAIVGTAALCVIAALGHLW
                     PLVLLGAWAVVAVAETILVRRHTAAVLKATTDGSTDE"
BASE COUNT          601 a         1004 c         1061 g          609 t
ORIGIN      
        1 gaccactagg agcgagaacc ccgaggagct gaagaacccg gggagatggt cgctcaggat
       61 ctccggcagc ggcagtcctt tcagaacgga gtcaaccgcg atcttcaggg ggatcggaga
      121 gagcaggaag atcggggtgg ctgccagcga gataagcaag acgccgacga tgtggatcca
      181 gtagcggcgg cattccttag tgagcctcca caccatgttg gcgtccgatc gccgttcggt
      241 cggctttttt cggcggcggg ctctccacca cgcctcgatc cggttccgcg acggcttctg
      301 cgcggtgacc gtaggaggtg gctccgattc gtcgagcgtg atgttgccgt aactgacttc
      361 atcgagcgcg gcgcgggccc aggcccatgc gtagaccaac aggagataca agacggccgg
      421 caccatcagc gcgagcgcgc cccatatcag cctcccgttg tcttgaaggt tcccgatgac
      481 catcaggccg gcagcgagag ccagtaggcc gaccgatgcc ctcgtcagcg acaggatgaa
      541 tgccgaatag ggagggtccg attcttcgat cttggtccaa ggatccttcc agatgtcgac
      601 cctgcgctgg agtagtcgcg gtctattcat cggtgcttcc gtctgtggtt gccttaagga
      661 ctgcggcggt gtgccgtctc acgagaatag tttcggcgac ggcgacaacg gcccatgctc
      721 ccagcaggac caacggccac aggtgaccca gcgccgcgat tacgcagaga gccgccgttc
      781 cgacgatcgc gggtgcccgc aatcgcatcc tcgaacggct cacgggcgtc cacccccaca
      841 ccaccgcgac gcttagtctc tgcgcgacgg ttaggccgaa ggaagtttcg atgtcccagt
      901 gatcgtgagg cccgccgatc ctcaccgata gatctttctc gcgcagcccg ttctcgatcc
      961 gccgtagcca gtggatcctc tcgccggtcc acagcgtggg cgccggcggc ttcccgcgct
     1021 tctcgacgga gcttctgtag aacacccgcc cccatgcgcg cacgatcggt tggctcaggt
     1081 agaagaaccc cacgagcgtt ctgtagcgga gcggatgagg ttcggttcga gtggggtgcg
     1141 cggcgacggt gactgcgatc cagtacgcca gagtgaggat catgctcgcg acgccgatcc
     1201 atctgccgac gctcgtgaag ggagctgcga gcgcgccgca gacggcgaat gccgcgaaca
     1261 gaggcaggaa tgcggttgcg agattgcgcg caacctcggc tcgacggtgg agcacgccct
     1321 gatagggggc catgccctga tagccgtggt agatgagtgg gcggagcaga gtcgcgagga
     1381 tccgggggcc tccatagatg aagcccgacc agcgtgcctg acccaatcgg ttgaatcggt
     1441 ggggatgttt ggccgcgacc atccgctcgg cgcgtccgta gcccctttgc tgcttcaggt
     1501 atcccttcac cgagctgcgc cggtggtgcc ttacctgggc ggcggccgag aaggagatgt
     1561 cccttcccgc gtcgaggagc ttccagcaga cgtcaacgtc atcgcccgcc gttgtatagg
     1621 cagggtcgaa gccttcaacc gcgaccagat cactctttcg gaaagccatg ttgcatcccg
     1681 gcacatgctc ggcgcgatcg tcggtgacta ggacgtgtat ggggcctccg ggcgaggctg
     1741 cgacggctct ctcgacgagg cccgcgttct ccaccggtag gttcgggccg ccggtcccgg
     1801 cgagactgtc gttctcgagt gatagggcga gatggaaagg ccatcccggg tggcagaagg
     1861 catcggagtc taggaacgcg acgatctggc cggtagccgc ggcgatgccg gcattgcgtg
     1921 cggcactcag accgccgtgc cccagctcaa ggacctgaaa ggggaaacgc cgtgctctct
     1981 cgagcgtgtc gtccgtcgag ccgtcgtcgc acacgatgac ctcgaggtcc ggataagagc
     2041 agttcgcaac cgaccgcagg cactgctcga tgtgttcacc cgcgttgtaa gcacagatca
     2101 cgaccgagac ccgcggccaa tggttgcgga ggtccgcgat gctcagagac gtccaccggc
     2161 ggaggacttc caagctctgc ttcggccgtc gctcggtatc cgtaactccg aagccccacc
     2221 cttcgacggc cttgttgttc accgcccact cgtcggtcca ggcgaagact gtggcaccgg
     2281 ccactccgca ttcgtccagt acgcgtagct gccagctcag cgccgccgct tgctcttcct
     2341 ctccgtggat cgcggatgcg agccccaatt cggtgaggat cagcggtctg tcgtcggcaa
     2401 caacctgcag atggcggatg tagcgcctga acgttctcgg gtcttcgagg aagacgttca
     2461 agcagacgat gtcctgcccg ctgatctgca ggaactcggt ggtggggaag ttgcagtaag
     2521 tggcgagcat ctcggggtct ccggagtgaa cggcatcgat cagctcggcg agcacacgtt
     2581 caaccgtaga gatgccgtga gtgcgggcca cgtcgacggg cacctcgttg ccgacggaca
     2641 gtgccaatac ttcgggccgt cctccgcaga gttctagggc ccgctcgaca gcacgaaggc
     2701 cggtatcacg gacgcggcgg ttggcagcgc ctccaggctg agtctcgtag cgccagtcga
     2761 gatagtcgac ccccacgatg accttgaggt cgagctcggc agcgacgtcg agcagctcga
     2821 cggggggaag cgtgtaagta cgaacggtgt tcaagccatg ttctttcatg gcgatgaggt
     2881 cgttcttcag cttccccgat tcgggaaaga gggctccgtc ggcccgggcc tcgaatgttc
     2941 cgtaagtgac tccctttact cggaatgggt ctcctccaag gagcaggtgg ttgccgtctg
     3001 ccctaacctt ctggtggagt tgtggatggc cgttggaaac gaatggggtt ggggagatcg
     3061 aaccctggga gcccacggac tcacgtgcga gcccctcaat atgaggagtc gcgccagtat
     3121 cggtagtcac aaatacccct cgctccgcca gaagcatttt tctcgcgttc tcggccccag
     3181 tcgaacattc tcgaataaag gggattttcc tacctcagcg gtaggaaatc ccagtcggtg
     3241 gtcggaaacc cttggtgggg cttgagatag gggcc
//
LOCUS       DATMED010000408         5305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3404525, whole genome shotgun sequence.
ACCESSION   DATMED010000408 DATMED010000000
VERSION     DATMED010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3404525"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /gene="dtd"
                     /locus_tag="VNP73_10670"
     CDS             <1..294
                     /gene="dtd"
                     /locus_tag="VNP73_10670"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWL66426.1"
                     /translation="RIFSDEQGKMNRSLLEAAGEALVVSQFTLYGDARKGRRPSFIHA
                     AQGPEAEAVYNDTVAGFRALGVTTATGRFGAMMMVDLVNDGPVTILLDSAKAF"
     gene            367..561
                     /locus_tag="VNP73_10675"
     CDS             367..561
                     /locus_tag="VNP73_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005870969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66427.1"
                     /translation="MAVLLRLQLWLQERHGDERGDVPGWVLIVLMTSALVVAIWGVAR
                     DRLVAIVSSALTTVCGSIGC"
     gene            566..949
                     /locus_tag="VNP73_10680"
     CDS             566..949
                     /locus_tag="VNP73_10680"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HWL66428.1"
                     /translation="MSGERGSAPVESTLALVFILFLSLGVIEVALALYARNVVLASAH
                     EGARAAAEIGRDESEAAAIAADTIRRSTGNLVRDLRVAVSVNDAGTSSTIRVLVTGVV
                     DAPGPVPFPLPIRATAGATTSGLPR"
     gene            946..1368
                     /locus_tag="VNP73_10685"
     CDS             946..1368
                     /locus_tag="VNP73_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66429.1"
                     /translation="MRRDEGGQAVVESILLCLVLLVPLIWMLGVLAELHRGALAAGAA
                     ARDAGFAAASAPDLRAASDAIDIAVRQAFVDHELDPERASVRWTSAPGLERGGIVEVR
                     VEYPVPVARIPFVDRVAGPSIRIDARHAARIDPFRSRG"
     gene            1365..1781
                     /locus_tag="VNP73_10690"
     CDS             1365..1781
                     /locus_tag="VNP73_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWL66430.1"
                     /translation="MKLRGESGQVAVLVLGFAIVVMAVVGLAVDGTKAFLYRRTLQSA
                     ADGAAVAGASELDLDSYYSSGGRVVAIAPGAARLQALRLLDSRAIRTTVAVDATEDAV
                     SVVIRGRVETTFLNLVGVSAIPVAVEAVAEPFAGES"
     gene            complement(1778..2632)
                     /locus_tag="VNP73_10695"
     CDS             complement(1778..2632)
                     /locus_tag="VNP73_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66431.1"
                     /translation="MTINLFPSLKVEEQDADRGTEGKGGTTSQLRSSAGTRFASLAAL
                     LCVALLVLSPTPAYAQIDVGDTVDDATDAVEDTVEDATETVEDTVDDATETVDEATDT
                     VDRATDEEGPVQEAVDTVTGTVDDATGGRTKPVTDVLRDTTGGALKTVDKTVEDIVDG
                     VMASQGPQFAPGSTRGSVRGQRVTANDRVKAGRSFGDTGTDDIADPATGTSADPIENT
                     SVVTPRSPEAGGTLGQIERIVRALAFPMILLVLIAGFLVIQGRLDRNDPKLTLAPLDV
                     DEEYLSFR"
     gene            2658..3629
                     /gene="argC"
                     /locus_tag="VNP73_10700"
     CDS             2658..3629
                     /gene="argC"
                     /locus_tag="VNP73_10700"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWL66432.1"
                     /translation="MGIQAAILGASGYTGGEVFRYLAGHPSIGVQAVAAGSTAGRPLA
                     ELHPGVAAVAGDLVLQPLEDVVGRSVDVLFSCLPSGRLKEHDAKLKADLVIDLADDHR
                     ADTEWVYGLTEFCRGDLQGASRIANPGCYPTAVLLGLVPFVRAGVVSDPVVVDAMSGV
                     SGAGRGLDDRLLFTNIEGGAAAYGAVPHRHVVEMERYAERFGGAEVHLSFTPHLIPVT
                     RGLVATIRAKLVHPITNEEAMEILNGAYAGEYFVHPRSEWPGTKAVAGSNHVHVSARV
                     DDRNGWLIASSAIDNLGKGAAGQAIQNANVALGLDETSGLQVLGVSP"
     gene            3626..4759
                     /gene="argJ"
                     /locus_tag="VNP73_10705"
     CDS             3626..4759
                     /gene="argJ"
                     /locus_tag="VNP73_10705"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWL66433.1"
                     /translation="MKWPQGFRSAGTHCGIKAAGASDLGLLVADGSVEWAGTFTTNAA
                     AAAPVQWSRSRLGSPVRAIVANSGNANACTGKAGAKAVDAGASRVAEALGCDPGEVLV
                     ASTGPIGVQLPVDLVSSGIERLPQLLSEDPEPFATSIMTTDTRLKVASAPVGAGSIVG
                     VAKGAAMCAPNMATMLAFLATDVKPPTEMQPALTDAVLQTFNRISIDGCESTNDSVFL
                     MSSAVHACDDLAFRSAVLDVCRELALAIASDAEGGTRLMRVQVSGAPTETDAVDLARA
                     VADSALWRAAFHGGDPNWGRVLSALGSARRDLDLTQVTVALGSETVFDRGEPAGSLEG
                     AAKAMTGDEVVVECIVGTGAGRAEVLSADLSADYVRLNAEGTS"
     gene            4756..>5305
                     /gene="argB"
                     /locus_tag="VNP73_10710"
     CDS             4756..>5305
                     /gene="argB"
                     /locus_tag="VNP73_10710"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWL66434.1"
                     /translation="MTDPAFEARSKAKILIEALPYIRSYRNQVVVVKIGGAPLDDEAL
                     ARLVAEDLALLTLVGIRLVILHGGGPQVTDAMTSAGIEPSFVGGLRVTDDDAIEVVRR
                     VLVGSINSALVARLNSAGLNAVGISGSDGGVFEAERTAGPAGEDLGHVGKIKVFRPDL
                     VTSLLNDGYTPVIASVAPDGEGN"
BASE COUNT          841 a         1627 c         1837 g         1000 t
ORIGIN      
        1 aggatcttct cggacgagca agggaagatg aaccggagcc ttctcgaggc agccggggag
       61 gcgctcgtcg tatcccagtt cacgctctac ggcgacgctc gcaaaggacg caggccctct
      121 ttcatccacg ccgcgcaggg gccggaggcc gaggcggtct acaacgacac ggtcgcgggg
      181 ttcagagccc tgggtgtgac gaccgccacg gggcggttcg gagccatgat gatggtggac
      241 ctcgtaaacg acggtcccgt cacgatcctg ctcgactccg ccaaggcttt ctgagcttgg
      301 tttgcctggg agcgaccggg ccttctagaa ttttcctgta gcgggctcat tccgggggga
      361 cgggagatgg cagtgctgtt gcgactgcaa ttgtggctgc aggaacggca tggcgacgag
      421 cggggcgatg tgcccggctg ggtcctcatc gtcctcatga cgagcgcgct ggtggtggcg
      481 atctggggag ttgctcgcga ccggctggtt gcgatcgtct ccagcgccct aacgacggtc
      541 tgcggcagca tcggctgctg aagcgttgtc gggggaacga ggatcggctc cggtcgagtc
      601 gacgctggcg ctggtcttca tccttttcct gtcgctgggc gtgatcgaag ttgcgctcgc
      661 gctttatgcc aggaacgtgg tgctcgcttc ggcccacgaa ggcgctcgcg cagctgcaga
      721 gataggtcgc gacgagtccg aggctgcggc gatcgcggcc gacacgatcc gcaggtcgac
      781 cgggaacctc gtgcgcgatc ttcgggtcgc cgtttcggtg aacgatgcag gtacttcatc
      841 cacgatccga gtgctggtca ccggggtggt ggatgctccc gggccggtcc cattcccgct
      901 tccgatccgt gcaaccgccg gagcgacgac ttcagggttg ccgagatgag gcgcgacgag
      961 ggtggtcagg ccgtcgtcga gtcgatcctt ctctgcctcg tgttgctcgt gccactcatc
     1021 tggatgctcg gtgtgctggc ggagcttcat cgaggtgcgt tggctgcggg ggcggccgcg
     1081 cgggatgcag ggttcgccgc cgcctcggcc cccgatctac gcgcagcctc cgacgctatc
     1141 gacatcgcag tacggcaggc cttcgtggat cacgagctcg atccggagcg cgcttcggtt
     1201 cggtggacct cggctcccgg cctcgagcgg ggcgggatcg tcgaagttcg ggtggaatac
     1261 ccggttcccg ttgcgcgcat cccgttcgtc gatcgggtgg ccgggccctc gatccggatc
     1321 gacgcccgtc acgctgctcg catcgacccc ttcaggagca gaggatgaag ctgagaggtg
     1381 agtcagggca ggtcgcagtc ctcgtattgg gctttgcgat cgtcgtgatg gcggtggtcg
     1441 ggctggcagt ggacggcacc aaggcgttcc tctaccggcg aacgcttcaa agcgcggctg
     1501 atggagcggc cgtggccggc gcaagcgagc tcgatctgga ctcgtattac tcgtccggcg
     1561 gacgggtggt cgcgatcgca ccgggggccg cgcgcctcca ggcgttgcgt ctgctcgatt
     1621 cacgcgccat ccgaaccacg gttgccgtcg acgctacgga agatgccgtg agtgtggtca
     1681 ttcgcggtcg cgtcgaaacc acatttctga atcttgttgg agtatccgcg atcccggtgg
     1741 ccgtcgaagc cgtggcggag ccgttcgcgg gagagagcta gcggaagctc aggtattcct
     1801 cgtcgacgtc gagcggggcg agcgtgagct tcggatcgtt cctgtccagc cgcccctgga
     1861 ttaccaggaa gccggcgatg agaacgagaa ggatcatcgg gaaggccagg gcacgaacga
     1921 tccgctcgat ctggcctagg gtgccaccgg cctctggact gcgcggagtc acgaccgatg
     1981 tgttctcaat cggatccgcc gacgtacccg ttgcgggatc tgcgatgtcg tcagtaccgg
     2041 tgtcgccaaa cgaacgtccc gctttcacgc gatcgttcgc cgtcacgcgc tgacctctga
     2101 cggaaccgcg cgtggaacct ggggcgaact gtggcccttg cgaagccatc acgccgtcga
     2161 cgatgtcctc cacggtcttg tcgaccgtct tgagggcgcc gccggtggtg tctcgcagaa
     2221 cgtcggttac cggcttcgtg cgacctcccg tggcatcatc gacggtcccg gtgactgtgt
     2281 cgacggcctc ttgtacggga ccttcttcgt cggtcgcgcg gtcgaccgtg tcggtggctt
     2341 cgtccacggt ctcggtcgcg tcgtcgaccg tgtcctcgac ggtctcggtt gcatcttcaa
     2401 cggtgtcttc gaccgcgtcg gtggcgtcgt cgacggtgtc accgacatcg atctgggcgt
     2461 aagccggggt gggagacagg accaggaggg caacgcaaag aagcgccgca agcgacgcaa
     2521 atctcgtgcc cgctgaggac ctaagctggc tcgtcgttcc tccctttcct tctgttcccc
     2581 tatcggcatc ttgctcctcg accttgaggc ttgggaagag gttgatagtc atgcagaaaa
     2641 ccgcatagac tctgcatatg ggcatccaag ctgcgatcct gggcgcatcg ggatacaccg
     2701 ggggcgaggt ttttcgctac ctggcggggc accccagcat cggggtgcag gcggttgcgg
     2761 ccggctctac cgccggacgc cccctggcgg agttgcaccc tggcgtggcg gcggtggcag
     2821 gagacctcgt cttgcaaccg ctggaagacg tcgtcggtcg atcggtcgac gtgctcttct
     2881 cgtgcctgcc gtcgggtcgc ctgaaggaac acgatgccaa gctcaaggca gatctcgtca
     2941 tcgatctggc cgacgatcac cgcgccgaca cagagtgggt ctacggactg accgagttct
     3001 gccgcggcga tcttcaaggc gcttcccgca tagcgaatcc cgggtgctat ccgactgcgg
     3061 tcctgctcgg gctcgttccg ttcgtgcgcg ccggggttgt gagcgatccc gtcgttgtag
     3121 acgcgatgtc gggagtctcg ggtgccggtc gcggtctgga cgacagactg ctgttcacga
     3181 acatcgaggg aggagcagcc gcatacggtg cggttcctca ccggcatgtt gtcgagatgg
     3241 agcgctatgc cgaacgcttc ggaggcgcgg aggtccatct gtcgttcacg ccgcatctga
     3301 ttccggtcac ccgggggttg gtggccacca tccgagcgaa attggtccac ccgatcacca
     3361 acgaagaggc gatggagatc ctgaacggcg cctatgccgg cgagtacttc gtgcatccgc
     3421 gcagcgagtg gccgggtacg aaagctgtcg ccggtagcaa tcacgttcac gtcagtgcgc
     3481 gtgttgacga tcgcaacgga tggctgatcg cttcttccgc gatcgacaat ctcgggaagg
     3541 gggctgcggg tcaggcgatc cagaatgcga acgtggcact gggtctcgat gagacatccg
     3601 gcctgcaggt cctcggggtg tcgccgtgaa gtggccccaa ggcttccggt cggcgggtac
     3661 ccattgcggg atcaaggccg ccggagcatc cgatctgggc ctcctggtcg ccgatggctc
     3721 cgtcgagtgg gcgggaacgt tcacgaccaa cgcggcggcc gcggcgcccg tgcagtggtc
     3781 ccgatcacgt ttgggcagtc ccgtccgggc catcgtcgcc aacagtggca acgcaaatgc
     3841 atgcacaggg aaagccggcg cgaaggcggt cgatgccggc gcgagtcggg ttgcggaagc
     3901 actcggctgc gatcccggcg aagtgctcgt ggcatccacg ggccccatcg gggtgcaact
     3961 gccggtcgat ctcgtgagct cggggatcga acgtctcccg cagcttttgt cggaagaccc
     4021 cgaacccttc gcgacctcca taatgacgac cgacacgcgc ctcaaggtcg cgtccgcacc
     4081 ggtgggcgcc ggcagcatcg tcggcgtggc aaaaggggcc gccatgtgcg ccccgaatat
     4141 ggccacgatg ctggcgttcc tcgcaaccga cgtgaagccg cccaccgaga tgcagccggc
     4201 tctcacggat gccgtcctac agaccttcaa ccggatctcg atcgacgggt gcgagagcac
     4261 caacgactcg gtctttctga tgtcgagtgc ggtacacgcg tgcgacgacc tggcgttccg
     4321 cagcgcggtc ttggatgtgt gtcgcgagct ggcgctagca atcgcatcgg acgcagaagg
     4381 tgggacgcga ctgatgcgtg ttcaggtcag cggagctccc accgagaccg acgcggtcga
     4441 tctcgcccga gcggtcgccg actctgcttt atggcgggcc gccttccacg ggggagaccc
     4501 caactggggg cgtgtgctgt cggcgttggg tagcgcgcgc cgggacctcg acttgaccca
     4561 ggtaacggtg gcgctcggct ccgagaccgt gttcgaccgc ggcgaacccg cgggttcgct
     4621 cgagggggcc gcgaaagcga tgaccggcga cgaggtcgtc gtcgagtgca tcgtcgggac
     4681 gggtgcgggc cgtgctgaag tgctgtcggc cgatctgtcg gccgattacg taaggctcaa
     4741 cgcggagggg acgtcgtgac ggaccccgcc tttgaagccc gcagcaaagc caagatcctg
     4801 atcgaggcgc tgccttacat ccgctcgtat cggaatcaag tcgtggtcgt gaagatcggg
     4861 ggagcgccgc tcgacgacga ggcgctcgct cggctggtgg ctgaggacct ggccctactc
     4921 acactcgtcg ggatacggct ggtgatcctt cacggcgggg gtcctcaggt cacggatgcg
     4981 atgacgtctg ccggtatcga accctccttc gtcggtggtc tgcgggtcac cgacgacgac
     5041 gcgatcgagg ttgtgcgcag ggtgctggtg ggatcgatca actcggccct ggtcgctcgg
     5101 ttgaactcgg ccggattgaa cgcggtaggc atctcgggtt cggacggagg tgtgttcgaa
     5161 gccgagcgca cggccggtcc ggccggcgaa gacctggggc acgttggaaa gatcaaggta
     5221 ttccgacccg atctggttac gtccctcctc aacgacggat acacgcccgt catcgcatcg
     5281 gtggcacccg acggagaggg caacc
//
LOCUS       DATMED010000409         2619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4543027, whole genome shotgun sequence.
ACCESSION   DATMED010000409 DATMED010000000
VERSION     DATMED010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4543027"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VNP73_10715"
     CDS             complement(<1..295)
                     /locus_tag="VNP73_10715"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWL66435.1"
                     /translation="MSEVLREEVTVDRSQSVAWAHLAHLEQWPTWATHIKRMEPHPPG
                     ELTASTQVVVRMRAGPRNKMTVTEYDPPHRWVWEGKAFGITTRFEHEFEALDEN"
     gene            491..844
                     /locus_tag="VNP73_10720"
     CDS             491..844
                     /locus_tag="VNP73_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66436.1"
                     /translation="MLQTLKQAWWAPLAIALSLAQFWVGLAFLYGEGTSNLLDAESTA
                     AGVSLAFGGAVALAAGLWIRPKARWAGNALIIVGAALGALWFWTVVMTPIAIVVIVGV
                     VVTQVRSTAPAARTT"
     gene            complement(805..1407)
                     /locus_tag="VNP73_10725"
     CDS             complement(805..1407)
                     /locus_tag="VNP73_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWL66437.1"
                     /translation="MSSTVLYMSMSLDGFIAGPNENPDRLHEWVFPGAEGGDFEAAVA
                     RLRGVNRQIYDEFMSTGAVVAGRGTFEPAGGWGGDHHDGVPIYILSRNPAPDWAAGWP
                     AVHYVSDLEAAMREAKNAAGDKNVLVHGSSIAQRAIAAGLLDEIEIHLVPILFGEGRR
                     LFEHLGVERRELERIRVLEGEAGVTHLRYVVLAAGAVERT"
     gene            complement(1417..2010)
                     /locus_tag="VNP73_10730"
     CDS             complement(1417..2010)
                     /locus_tag="VNP73_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWL66438.1"
                     /translation="MRLVLSEFISLDGVIQAPGGRKEDTDGGFQHGGWSMRYFDQDAM
                     GSAIGEAMEKTDAYVFGRKTWQVSAEAWPQREGQDPFADKLNSIPKHVASRSLSQDDL
                     TTWNNSHLLPGDDALGAIAELKERDGGDISCYGSANLAAQLIANDLVDELNLMIEPVI
                     LGGGKRLFPEDGVLRGLELVSSTAAGTGVQICKYQRA"
     gene            complement(2085..>2619)
                     /locus_tag="VNP73_10735"
     CDS             complement(2085..>2619)
                     /locus_tag="VNP73_10735"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWL66439.1"
                     /translation="SGNIMPPNGNIPADDNPAAPRENGDPHSHGRDEYAAGWQEAHFL
                     LTGWMEDVCGGGDYLTRRHQDNNGTASCIPPEWAPGNKPGDEQSPTGLTLTVTGKGSK
                     TSLEAVLLDMDSGAGVHGRIIEFFAGGNPIGTAITGNDGRARLPIPSRYRGGHHDFEA
                     RFLGDDAYGPSTGSART"
BASE COUNT          423 a          841 c          809 g          546 t
ORIGIN      
        1 ggttctcatc caaagcctcg aactcgtgct cgaagcgcgt cgtaatgccg aacgcttttc
       61 cttcccagac ccagcgatgg ggcgggtcgt actcggtgac ggtcatcttg ttgcgcgggc
      121 ctgcccgcat gcgcactaca acctgagttg aggccgttag ttccccgggg ggatgtggct
      181 ccatccgctt gatgtgagtg gcccaggtcg gccactgttc gagatgtgcg agatgggccc
      241 acgccaccga ctgagaccga tcgacggtta cttcctcgcg caatacctca ctcaagttgg
      301 atccttcgtg gacggcgtga tccgcagctt ttcgaaggca agtttcctcg aacggaggcg
      361 acattgcgag cgcggcggcg tacgatagat aggaggaacc ccgcttcagt gggctgcccc
      421 tacccctggg ttgcaaccgg ccgtcgcccg caccctcctg gaccctgccc tctgacggaa
      481 ggaagccgcg atgttgcaaa ccctgaagca agcttggtgg gccccattgg cgattgcgct
      541 gtcgctcgct caattctggg tagggctagc gttcctgtat ggagagggaa cttctaatct
      601 cctcgacgcc gaaagtacgg ctgccggcgt ctcgctcgct tttggcggtg ccgtcgctct
      661 tgccgccgga ctctggatcc ggccgaaagc tcgctgggcg gggaatgcgc tgatcatcgt
      721 cggcgcagcg ctgggcgcgc tttggttctg gactgtggtg atgaccccga tcgcgatcgt
      781 ggtcatcgtg ggggtcgttg tcactcaggt gcgttcaacg gccccggcag cgagaaccac
      841 atagcgaaga tgtgtgacgc ctgcttctcc ttcgagaacc ctgatccgct ctagctcgcg
      901 ccgctcaaca ccgaggtgtt cgaacagcct gcgtccttca ccgaacagga tagggaccaa
      961 gtggatctcg atctcatcga gtagccctgc ggcgatcgcg cgttgcgcga tgctcgagcc
     1021 gtgcaccagc acattcttgt cgccggccgc attcttggcc tctcgcatgg cggcttccag
     1081 gtcgctcacg tagtgcacgg cgggccatcc agcggcccag tcgggggccg ggttgcgact
     1141 caggatgtag atgggaacac cgtcgtgatg atcgcctccc catccaccgg caggttcgaa
     1201 ggtcccacgt ccggccacca cggcccccgt ggacatgaac tcgtcgtaga tctgtcggtt
     1261 cacgcctcgt agccgcgcaa cggctgcctc gaagtcgccg ccctcggcgc ccgggaagac
     1321 ccactcgtgt agccgatccg ggttctcgtt cggtcccgcg atgaatccgt ccagggacat
     1381 cgacatgtac agcacggttg aggacatcgc tttctcctat gcccgctggt acttgcagat
     1441 ctggacgccg gtgccggcgg ccgtgctcga aacgagctcg agacccctca gcacaccgtc
     1501 ctccgggaag agccttttgc cgccgccgag gatgaccggc tcgatcatca ggttcagctc
     1561 gtcgacgaga tcgttcgcga tcagctgagc ggcgagattg gcgctcccgt agcacgagat
     1621 gtcgcctcca tctcgttcct tcagctctgc gatcgcaccg agagcgtcat cgccgggcag
     1681 caggtgcgag ttgttccatg tggtgaggtc atcctgcgac aagcttcggg atgcgacgtg
     1741 cttcggtatc gagttgagct tgtcggcgaa gggatcctga ccctctctct gcggccatgc
     1801 ctccgcggag acctgccacg tcttgcggcc gaagacgtat gcatcggtct tctccatcgc
     1861 ttcgccgatc gcggagccca tcgcatcctg gtcgaagtag cgcatcgacc acccgccgtg
     1921 ctgaaaccca ccgtcggtat cttccttgcg cccgccgggg gcctggatga ctccgtcgag
     1981 gctgatgaat tcactgagaa cgagacgcat cgagcaactc ccttctatgt ggtgatcggt
     2041 agttgctcgt tagactctat gaagctcaga aattcatcgc cagcctaggt tcgggcggag
     2101 ccggtagacg gcccgtaggc atcgtcgccg aggaaacggg cctcgaagtc gtgatgaccc
     2161 ccccgatatc tcgacgggat cgggagtctc gcccggccgt cgtttccggt tatcgccgtt
     2221 ccgatgggat tgccgccggc gaagaattcg atgatccgac cgtggactcc ggcccccgaa
     2281 tccatatcga ggagcacggc ttcgagtgag gtcttcgatc ccttccccgt gaccgtcagg
     2341 gtcagccccg tcggtgactg ctcgtcgccg ggcttgtttc cgggtgccca ctccggcggg
     2401 atgcaactcg cggtgccgtt gttgtcctgg tgccgacgcg tcaggtaatc gcctccgcca
     2461 cagacgtcct ccatccaacc ggtcagcagg aagtgagcct cctgccaccc ggccgcgtat
     2521 tcgtcacgtc catggctatg cggatcgccg ttctcccggg gagctgcggg gttgtcgtcg
     2581 gcggggatat ttccgttcgg gggcatgatg ttgccgctg
//
LOCUS       DATMED010000410         5884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1473377, whole genome shotgun sequence.
ACCESSION   DATMED010000410 DATMED010000000
VERSION     DATMED010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473377"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VNP73_10740"
     CDS             complement(<1..214)
                     /locus_tag="VNP73_10740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWL66440.1"
                     /translation="MLKLGIACLVFLAAIACSNGADCEQGVIETDSGLKYEEIECGDG
                     AEAARGDTVSLHYTGTLEDGEEFDSSE"
     gene            complement(870..1568)
                     /locus_tag="VNP73_10745"
     CDS             complement(870..1568)
                     /locus_tag="VNP73_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66441.1"
                     /translation="MIHVAKAAPGVASAGSGALFGLLLLTEAGLPIPIPADLLMLLLG
                     ERVSAGALPLAGVLIALQAIAILGTLGLFWVVRGPGRQVLMRFGPKVGVTEARIERVS
                     AMVAKRGTPAVALGRSTPGLRTVTVVAAAGTGRSDTRLLLALIAGSTLFVQGHFLLGL
                     ALGPAARALFEAGGKVAVVVLLALLVIGGIAVWFRRRRGSGGLQSFSEACCPACLAVG
                     LVYQRSEIRRSSGA"
     gene            complement(1671..3572)
                     /locus_tag="VNP73_10750"
     CDS             complement(1671..3572)
                     /locus_tag="VNP73_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HWL66442.1"
                     /translation="MSLSRRGKLTLWAGLALVALLTTSVPVAATPVCPDEPRAACGGR
                     IFPEAENAVAFVQHDSGEYADGIAALARDFPRFVKVRTFSDVLGREALSFGGREMRLV
                     EITDFEAPEEDKIPVVVSMSVHGPERAGLEGGVRYMEDLARWAEENRDRVLRNGTEKD
                     SIGVPVRDVLERVHLYLADANPDGWAEGDTQNGGVFMRGNGNGVDLNREFPTIGWSYP
                     PYTALSEPESIAWAEIVRGIDPVITSDMHGELTSAQDAFADMMYPVGQWDPLMQARQE
                     RLARHMKSNVDRYFEEKGVEAQQVSGFAGMKPAEYATGFDVVGYDDAGFMGDWFTQEF
                     GAVDMDVEHFFSHMAPNSTWAAPLEEAHIEAVRGEIEAMIVEATVTHDIKVKMKVGRV
                     GYLSDPRVVKMSDGYGGGDVPEDVEPQAYSATRMQYFKDMSRYTTSRVRPVTVKRLSA
                     AKLRRFDSFVVADGFRELTKRRAAVLHQWVRRGGNLILTDQALQVVAKMGLVKKRAVK
                     KTLMGAGHIDIHQWDDLYVKGVHPTASQTYYEVPLGFTVDSDSSPHWTIARSAWRKAR
                     GKSAAHLEEENRIILGRAKLGKGRIGIFGAVLPQPTEKYEHFYGLADYAVSVAGGQIL
                     NNMFKAGVR"
     gene            complement(3563..4930)
                     /locus_tag="VNP73_10755"
                     /pseudo
     CDS             complement(3563..4930)
                     /locus_tag="VNP73_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            complement(5026..>5884)
                     /locus_tag="VNP73_10760"
     CDS             complement(5026..>5884)
                     /locus_tag="VNP73_10760"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWL66443.1"
                     /translation="QTAASGETLEISDSRFSGNTTGVALSDSPDPTVIFRHNYVDGNT
                     TGVLVNNSLSSFRLNTITDNETGIDFRAAGVFNDNNIFDNSDNNATACETLGPEDPEI
                     DADNNWWGLTDEGEIEDTICDLEDEDGVRKIKFEPFEMAPVPGAATSGPPASPSPSVT
                     TSPTGSTSPSPTSSPSPTQPPPPAEHARSVSLRLARHLKARGKVTVNDGFQGCMPASV
                     NVRYRRRAGQPWKSVARPVVNSSTGNYSARVQDKPGVYQTKVAQSQSSSDICRPASSP
                     KKRHGRHRR"
BASE COUNT         1157 a         1912 c         1732 g         1083 t
ORIGIN      
        1 cctcagaaga gtcgaactct tcgccgtcct ccagggttcc ggtgtagtga agcgaaaccg
       61 tgtcgccgcg cgcggcctcg gccccatctc cgcactcgat ctcttcgtac ttgagacccg
      121 agtcggtttc gatcacgccc tgctcgcaat cggcgccgtt cgagcacgcg atcgcggcca
      181 ggaacacgag gcaggcaatt ccgagcttca acacgcccgg caccctaacc gggggttccg
      241 atgcaatgtt tctggccgga ggggcgtctt atccatagtg agaagcttta cgcccaaacg
      301 ccgggagatc cttcctctat gaagacgcat cggaagcggt atctggtcat aggcctccta
      361 gcatgtttgt gcatcaataa tcccgctctc gcgcgggaaa caaccgatga cgacgacact
      421 gaggggcgcc tcgacataca gatggctcgc ggggaacgct tcaagaagaa cggcacatct
      481 tggtggaggt tcgagtttca agctcacgag tctttccgca accgcgatct aagacccgtc
      541 ggttcggagg agtcgtacaa agccctcact ttccatttca acactcgccc gggtggaggg
      601 ttcgaccgag ttgtcttcct gcaatctcga agaaaaggca gtacctacca agtcttttct
      661 cgcgtcacga agggtcgctc ggacaccggc ggcagatggg gttctggaac tactttggaa
      721 ggtaaggcgc aggtgtcgcg aagagggtcg tcatcgatcc agatcagatt tcgggaatcg
      781 ttgctgggac taagggctaa gcatttcgag tgggcagtcc ggactgcaaa tgacacatcg
      841 accgtgacga cctcccaaat cagctttgac tacgcgcccg aggaacgtct gatctcggaa
      901 cgctggtaga ccaagccgac cgccaaacaa gccgggcaac aggcttcgct gaagctttgc
      961 aggcccccag agccacgccg ccgtcggaac cagaccgcga tgcctccgat gacgagtaaa
     1021 gcgagcagaa cgacgacggc cactttgcca cccgcctcaa acaaagccct agccgcgggc
     1081 cccagcgcga gtcccagcag gaaatgaccc tggacgaaca gggtgctccc tgcgatcaaa
     1141 gccaggagga gccgagtatc cgaacgccca gttcccgccg ccgccaccac ggtcaccgtt
     1201 cgcaatccgg gggtgctgcg gccgagagct acggcaggag tccctctctt ggccaccatc
     1261 gcggacacgc gctcgatcct ggcttcggtg actccgacct tggggccgaa cctcatcagt
     1321 acctgccgtc ccggcccccg cacgacccag aagagcccga gcgtgccgag gatcgcgatc
     1381 gcctgaagag cgatcaacac gccggctaag gggagcgccc cagccgagac acgttctccc
     1441 aagagcaaca tcagaagatc ggccgggatc ggaatgggca gccccgcttc ggtcaacagg
     1501 agcaagccga acagcgctcc gctgccggcc gacgccactc cgggtgcagc tttagcgaca
     1561 tggatcacca gtcaccccgc catccatttg catcgggatc gcacgaacca ggttcgatcc
     1621 tcgagtgggc gtggatggga catatggtcc cgctcacgcc cactcggcgc ttagcgaacg
     1681 ccggctttga acatgttgtt caggatctgg ccccctgcaa cgctgactgc gtagtcggca
     1741 agtccgtaga agtgctcgta cttctccgtc ggctgcggaa gcacggcccc gaagatcccg
     1801 atccggccct tcccaagctt ggctcggccc aggatgatcc ggttctcctc ttccaggtgc
     1861 gcagcgctct tcccgcgcgc cttgcgccat gcactcctgg cgatcgtcca atgaggcgag
     1921 ctgtcgctgt cgacagtgaa tccaagaggc acctcgtaat aggtctgact cgcggtcggg
     1981 tggacgcctt tcacatagag gtcgtcccac tgatggatat cgatgtggcc ggcccccatc
     2041 agggtcttct tcacggccct tttcttgacc agacccatct tcgcgacaac ttgcaaagcc
     2101 tggtcggtca agatcaggtt cccaccgcga cgaacccact gatgcagaac agcggcgcgc
     2161 ctcttcgtca gctcacggaa accgtctgcg acaacgaacg aatcgaacct ccgaagtttg
     2221 gctgccgaaa gtctcttgac ggtgacgggt cgaactcgag aggtcgtata gcgagacatg
     2281 tccttgaagt actgcattcg ggtagcgctg tacgcctgcg gctcgacgtc ttccggaacg
     2341 tcccctccgc cataaccatc cgacatcttc acgacgcgcg ggtccgagag gtagccgacg
     2401 cgacccacct tcatcttgac cttgatgtcg tgggtaacgg tcgcctccac gatcatcgcc
     2461 tcgatctcgc ctcggacggc ctcgatgtga gcttcctcga gaggagcggc ccacgtcgag
     2521 ttcggcgcca tgtgagagaa gaaatgttcg acatccatat cgacggcacc gaattcctga
     2581 gtgaaccagt cccccatgaa tccggcgtcg tcgtaaccga ccacatcgaa acccgtggcg
     2641 tactccgccg gcttcatccc ggcgaagccg ctgacctgct gtgcctcaac gcccttctcc
     2701 tcgaagtagc gatcgacgtt cgacttcatg tggcgagcga gccgttcctg gcgggcttgc
     2761 atcaacggat cccattgccc caccggatac atcatgtcgg cgaaggcgtc ctgagccgaa
     2821 gtcaactcac cgtgcatatc actcgttatc accggatcga tgcccctcac gatctccgcc
     2881 catgcaatcg attcaggctc cgacagagcc gtgtagggcg ggtaggacca cccgatcgtc
     2941 ggaaactccc ggttcagatc cacgccgttc ccgttgccgc gcataaacac gccgccgttc
     3001 tgggtgtcac cctccgccca tccatccggg ttcgcatccg caagataaag atgcacacgc
     3061 tcgagaacgt cgcggaccgg cacgccgatc gagtccttct cggtcccgtt tcggaggacg
     3121 cggtcacgat tctcttcagc ccaacgcgca agatcctcca tataacggac cccgccctcg
     3181 agacccgcgc gctctggtcc gtgaaccgac atagacacga cgacgggaat cttgtcttcc
     3241 tcgggggcct cgaagtcggt gatctcgacc agcctcatct ctcggccccc gaacgacaac
     3301 gcctctcttc ctaagacgtc ggagaaagtc cgaaccttca cgaaacgtgg gaaatctcgg
     3361 gcaagggcag cgatcccatc cgcgtactca ccgctgtcgt gctgaacgaa tgcaaccgcg
     3421 ttctcggcct cgggaaagat gcgcccaccg caagcagcac gtggctcgtc ggggcacacc
     3481 ggcgtcgcag ccacgggcac cgaagtcgtc agcagtgcaa cgagagctag ccccgcccag
     3541 agtgtgagct tgcccctgcg actcagggac acggcgtcaa cagcggacag acgcgataac
     3601 gggccaggcg cgtcgaaaga tcggccacgt tggggtcgtt ggcggattcg gcgtcgccca
     3661 aaaggttctc cagttggtag ggatcggcgg tcaggtcgta gtactcgcgg aacgtagtaa
     3721 tcgtctcatc tccgccgtaa tactcgacgt actgaaaatc ggccgacagg atcgaggccc
     3781 acctgagctc gggtcgtgaa acggcaccgt aaacctcggt caacagttcc gaccggtccc
     3841 agacctcgag caacgagcgt ccgtcgaccg ggtgatcggg ggtgatcccc gcggcatcca
     3901 acaccgtcgg agccacgtcg atgttggccg cgagcctcgc atccacaccc ggaaccacgt
     3961 ggccatccca cttcaggaac atcgggatat cgaccgaggg cgcgtagggg gtggccttgg
     4021 ccgtaagccc gtgctcgccc cacatgtatc cgttgtccga catgaacacg atgagcgtgt
     4081 cctcctcgcc gcgcttcacg agctcggctc tgaggcgttc gaccatgtcg tcgacgggca
     4141 tcagcgcccg catctgccgg ccgcgcctcc cctcctggtc tgcatagtcg aaaggcttcg
     4201 cgtacttcga gtactgaggc ggcttgtccg acaggtcgtc ctcgagcatc gcgggatccg
     4261 gctcgaaggg tgggacctcg gcatcccaat accgcggcgg cgagatcgaa ggaccgtgtg
     4321 gagcgaacgg cgtcacgaac agcaaccacg gctcgacgtc gctcgcctcg gaccgttcca
     4381 ggaaccgaag ggctttccgc ttcacgaagt tcgtcgagta cccgcgactc cagcgaacct
     4441 tctgcgtccc ttcgacgccc aacaggaagc gcgagtggta atacccgcca cgggtgatcg
     4501 cgtactcgtc gaagtacggc ggtgcccggt tgacgtccca gccgttcaga aacttcccta
     4561 ccaaccctgt tctgtatccc ccccgctcgt gaagttagta ctgcaccgtc gtgtcgtgcg
     4621 agaggttctg cgcctcgccg tccgcgttgg agcgaacgtc gtggttgtgg gcgtactgac
     4681 ccgtgaagat cgacgcgcgc gatgggcaac agacgggtgt cgtggccacc gcatccgaga
     4741 actcgacgcc ggtcgagaag agatcgagag tcttgggcat cacgtccatc gtgccgtcgc
     4801 gctggtcgtc ggtaacgatg acgaggatgt tcggagccgg ttcctgggcg tcggcaggca
     4861 gagtccgccc cgcgcccaac aggggcagga aaaggacaat caaaagagag agcttccgcc
     4921 aagcgcccat cacaagaggc taacgccgcg gaggaccaga ggggtcacgt attgaggtag
     4981 ccgagggacc cgcatggagc aggcccctcg gccattagct atggcttacc gccggtgccg
     5041 cccgtgtcgt ttcttgggcg acgacgccgg gcgacatatg tcactcgacg actggctttg
     5101 agccaccttg gtctggtaaa cgcctggttt gtcttgcacc cgagctgagt aattgccggt
     5161 agaagagttc acgaccggcc gcgcgaccga cttccacggt tgacccgctc gacggcgata
     5221 tctcacgttg accgacgccg gcatacaacc ctggaagccg tcgttgacgg tcactttccc
     5281 cctcgccttc aggtgccgcg ccagcctcaa cgaaaccgac cgagcgtgct cggccggagg
     5341 cggaggctgc gtcggcgacg gcgaggaggt cgggctcggg gacgtggagc cggtgggaga
     5401 ggtggtcacg ctcggggacg gagacgccgg aggcccactg gttgcggcgc cgggcaccgg
     5461 ggccatctcg aaaggctcga atttgatctt gcgaactccg tcctcatcct ccagatcgca
     5521 gatcgtgtct tcgatctcac cctcgtcggt gagtccccac cagttgttat ccgcgtcgat
     5581 ctcgggatcc tcaggaccga gggtttcgca agcggtcgcg ttgttgtcac tattgtcgaa
     5641 gatgttgttg tcgttgaaga cacccgcggc acggaaatcg atccccgttt cgttgtccgt
     5701 gatcgtgttc agacggaacg aactgaggct gttgttcacc aagacgcccg tcgtattccc
     5761 atccacgtag ttgtgcctga agatcacagt cgggtctgga gagtcgctga gcgcaacgcc
     5821 ggtcgtgttc ccggagaacc tgctgtccga gatctccaag gtctcgccgc tcgcggcggt
     5881 ctgg
//
LOCUS       DATMED010000411         3455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_335145, whole genome shotgun sequence.
ACCESSION   DATMED010000411 DATMED010000000
VERSION     DATMED010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335145"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VNP73_10765"
     CDS             <1..206
                     /locus_tag="VNP73_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66444.1"
                     /translation="GARLERVLEEIKDTLVSAQRSEDTFVVTAGGTMFHRPDCKLVTG
                     RDDLREVAAAEDGLKPCKLCSPA"
     gene            286..1881
                     /locus_tag="VNP73_10770"
     CDS             286..1881
                     /locus_tag="VNP73_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009713280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWL66445.1"
                     /translation="MEFNFADLFESVVDAVPDREALVCGDRRLTYEQLDERATRLAHH
                     LAGVGVEAGDHIGLYMYNGTEYVEAMLACFKIRAVPINVNYRYVENELRYLFDNADLK
                     GLIHQQEFCPRIAAVKDELPLLKTFIVVEDSSGADYSVLGSVEYEKALSGASPERDFG
                     PRSSTDLFIIYTGGTTGMPKGVVWRHEDLFFAGMGGGNPLGEPIAKPEELAPNAREKD
                     VVVQFPVPPLIHGAAQLTVLIGFNWGGKIVLAPRFDPDGTWDLIEKEKVNTIMLVGDA
                     IARPLADTLSATKDQRDVSSLFYISSAGAVLSDPVKDQLKELLPNTIVAESFGASETG
                     FQGMESGSGRMRFVMNDRTTVLNDDLKPVEAGSGDIGRVAQKGRVPMGYYNDEEKSAE
                     TFVEADGDRWVLLGDMAMVEADGSITVLGRGTVCINSGGEKLFPEEIENAVKSHPDVY
                     DVVIVGIPDERWGEKVAALIEVRPGATLTLESIQEHCRKLIAGYKVPRALRIVEKVER
                     HPSGKPDYPWAKKVAAEETLEVT"
     gene            1878..2990
                     /locus_tag="VNP73_10775"
     CDS             1878..2990
                     /locus_tag="VNP73_10775"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HWL66446.1"
                     /translation="MKTSLTEMFGIEYPIFAFTHSPKVAAAVTRAGGMGVFGALSYTP
                     EQLREHLDWINDNVDGKPYGVDVVMPASYEGADLSDPEALISQLETMIPGEYKEFVAD
                     LLERNNIPELPEDADEARALLGWTEATARPQVEIALSYPIALLANALGPPPKDIVDEA
                     HAKGVKVAALVGHVDHAKAQVEVGVDIIVSVGTEAAGHTGEISTLVLTPQVVDAVAPT
                     PVLAAGGIGNGRQMAAALALGAQGVWTGSLWLTVEETDVDPKVVDKLLASTSRDTIRT
                     RAFTGKPARLLKSGWTEAWENKDNPDPLPMPLQYFLTAEAQHRIFRDGDNELLTFPVG
                     QIVGVMNEVKSTAEVIEAMVKECEEAAGAIDKTLGS"
     gene            complement(2987..>3455)
                     /locus_tag="VNP73_10780"
     CDS             complement(2987..>3455)
                     /locus_tag="VNP73_10780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWL66447.1"
                     /translation="RPPKIAELPDLNDLHLFFNDDPISGRDNPIAPPIEITFGDGAVE
                     GLVTFSRSYEGAPGFVHGGAIAAAFDQVLGMANLTSGHPGMTGTLTIRYISPTPTETE
                     IRFEATAGEKDGRKSFVTGACYDGDTLTAEAKATFVMLRFKKAAKMFLGRTAS"
BASE COUNT          690 a         1031 c         1117 g          617 t
ORIGIN      
        1 aaggtgcgcg cctggagcgg gtgctcgaag agataaagga cacgctcgtc tccgcccaaa
       61 ggtcggagga cacgttcgta gtcaccgccg ggggaacgat gttccaccga ccggactgca
      121 aactcgtgac cgggcgggac gacctacgcg aggtggcagc cgccgaagat gggctgaagc
      181 cctgcaagct ctgtagccca gcctgatcct cccgagccct cggctcgcct tgccgccaaa
      241 acgagaacgt gttctaattt ctcgcccggg aggagggcag ggaaattgga gttcaacttc
      301 gccgatctat tcgagtcggt agtcgacgcg gtacccgatc gcgaggctct cgtgtgcggc
      361 gatcgccgct tgacctacga acaactggac gaacgcgcga cgcggcttgc gcaccatctc
      421 gccggtgtcg gggtcgaagc cggagaccac atcgggctct acatgtacaa cgggaccgag
      481 tacgtcgaag cgatgctcgc gtgtttcaag atccgtgcgg ttccgatcaa cgtgaactac
      541 cggtacgtag agaacgagct ccgctatctg ttcgacaacg ccgacctcaa aggcctcatc
      601 caccagcagg agttctgtcc ccgcatcgca gcggtcaagg acgagcttcc gctactgaag
      661 acgttcatcg tggtggaaga ctcgagcggc gccgactact cggtcctcgg ttccgtcgag
      721 tacgagaagg cgctctcggg agcctcgccc gagcgtgact tcggtccccg ttcgagcact
      781 gacctattca tcatctacac cggtggaaca acgggcatgc ccaagggtgt cgtctggcga
      841 cacgaagacc tcttcttcgc aggtatgggt ggtggtaatc ctctcggcga gccgatcgcg
      901 aagccagaag agttggcgcc aaacgcgcgc gagaaggatg tggtcgttca gttcccggtc
      961 ccgccgctga tccatggcgc cgcgcaactc acagtgctca tcggtttcaa ctggggcgga
     1021 aagatcgtgc tcgcgccgag atttgacccg gacgggacat gggacctcat cgagaaagag
     1081 aaagtcaaca cgatcatgct cgtcggcgat gcgatcgcgc gtccgttggc agacaccctg
     1141 agtgcaacca aggaccaaag agacgtgtcg tccctcttct acatcagttc ggccggagcg
     1201 gttctctccg atccggttaa agaccaactg aaggaactgc ttcccaacac gatcgtggcg
     1261 gagagcttcg gtgcctcgga gaccggattc cagggcatgg agtcgggctc cggtcgcatg
     1321 cgcttcgtga tgaacgaccg gacgactgtt cttaatgacg acctgaagcc cgtggaagca
     1381 ggttccggtg acatcggccg tgtcgcgcaa aagggccgtg tcccgatggg ctactacaac
     1441 gatgaggaga aatccgcaga gactttcgtt gaggccgatg gcgatcgctg ggtccttctc
     1501 ggcgacatgg cgatggtcga ggccgacggc agcatcaccg tcctcgggcg gggaaccgtt
     1561 tgcatcaact caggcggaga gaagctgttc cccgaggaga tcgagaacgc cgtcaagtcc
     1621 cacccggacg tctacgacgt tgtgatcgtc ggcataccgg acgagcgatg gggcgagaag
     1681 gttgctgcgc tcatcgaggt acgacccgga gcgacgctga cactcgagtc gatccaggag
     1741 cattgccgca agttgatcgc cggttacaag gtcccgcgcg ccctgcgcat cgtcgagaag
     1801 gtcgagaggc acccgagcgg caaaccggat tacccgtggg cgaagaaggt tgcggctgaa
     1861 gagacgttgg aggttacgtg aagacttctc tgaccgagat gttcggcatc gagtacccga
     1921 tattcgcttt cacccacagc ccaaaggtcg cagcggccgt cacccgagcc ggggggatgg
     1981 gggtgttcgg tgctctctcg tatacacccg agcagttgcg cgagcacctc gattggatca
     2041 acgacaatgt cgacggcaag ccgtatggcg tcgatgtcgt gatgccggcg tcctacgaag
     2101 gtgccgacct ctcggatccc gaagcgctga tcagccagct cgagacgatg atccccggcg
     2161 agtacaagga gttcgtcgcc gatctgctgg agcgcaacaa catcccggag ctgccagagg
     2221 acgcggatga ggctcgcgcc ctgctcggat ggacggaggc gacggcgcgc cctcaggtcg
     2281 agatcgcact ctcatacccg atcgcactgc tggccaacgc gctggggcct cctccgaagg
     2341 acatcgtcga cgaggctcac gccaaaggcg tcaaggttgc ggctctcgtg ggtcacgtcg
     2401 atcacgccaa ggcgcaggtg gaggtggggg tcgacatcat cgtctcggtt ggaaccgagg
     2461 cggccggtca caccggtgag atctcgaccc tcgttctcac acctcaagtc gtcgacgcgg
     2521 tcgccccgac gcccgtgctc gccgcgggcg ggatcggcaa cggtcgtcag atggcagcgg
     2581 ccctcgcctt gggagcccag ggagtgtgga ccggttcgct ctggctcacc gtcgaggaga
     2641 ccgacgttga tcccaaggtc gtggacaaac ttctcgcctc aacttcaagg gacacgatcc
     2701 ggacccgggc attcaccggt aagcctgcgc gtttgctgaa gtcgggctgg accgaagcgt
     2761 gggagaacaa ggacaatccc gatccgctgc cgatgccgct gcagtatttc cttacggccg
     2821 aggcccagca ccgcatcttc cgcgacggtg acaacgagtt acttacattc cccgtcggtc
     2881 agatcgtcgg cgtgatgaac gaggtgaaat cgaccgccga ggtcatcgaa gcgatggtca
     2941 aggaatgtga agaggctgca ggggcgatcg acaagaccct cggcagctag gaggcggtgc
     3001 ggccgaggaa catcttggcc gccttcttga agcgaagcat cacgaacgtc gccttggctt
     3061 cggccgtgag cgtgtcaccg tcgtaacagg caccggtcac gaaagacttc cgcccgtcct
     3121 tctcgccggc tgtggcctcg aagcggatct cggtctcggt cggcgttgga ctgatatatc
     3181 ggatggtgag cgtgcccgtc atgcccgggt ggccgctggt caggtttgcc atgcccagca
     3241 cctgatcgaa ggcagcggca atggcgcctc cgtggacgaa ccccggagct ccttcgtatg
     3301 aacgggaaaa ggtgacgagg ccttcgacgg ccccatcgcc gaaggtgatc tcgatggggg
     3361 gagcgatcgg attgtcgcga cccgatatcg ggtcgtcgtt gaagaacaag tgcagatcgt
     3421 tgaggtcggg aagctctgcg atcttcgggg gccgg
//
LOCUS       DATMED010000412         4051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4546132, whole genome shotgun sequence.
ACCESSION   DATMED010000412 DATMED010000000
VERSION     DATMED010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546132"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..1761)
                     /locus_tag="VNP73_10785"
     CDS             complement(304..1761)
                     /locus_tag="VNP73_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWL66448.1"
                     /translation="MELAGIFALVAVVLTVSALAAGVVERAPISFPMIFLGLGFLLGP
                     LGLEVMDLDVHSPSLEAVATLTLALVLFLDAVNLEFARERKEWMVPALSLGPGTLVIV
                     ATVAGAGMWLLDMPPVAAFLLGAVLSSTDPVVLRDIVRDRRLPASVRQALRVEAGTND
                     VVVLPLVIVLAAVARGEVGGTGDWFLFALQLLVLGPAAGFAVGAIGSDLMSRADAHFG
                     IRREYQALYGVGLVLGAYACGVAVGGDGFLAAFAAGFAVTILDRELCDCFLEFGEAAA
                     EIAMLLAFVMFGVAVSASLSLLPVWQTVALAILTIVVARPLALGLLLSLRGVALSTPA
                     RAFIAWFGPRGLNSMLFALLVVVSGVPGSELLFAVTGAVVIASVLAHGVTATPVTGWY
                     ARRVAAETLAEEREDTASGLFVSHVDDVERITVEDLHTLLGGASPPLVLDVRSRSQYQ
                     RDAGAIPGSIRVMLDDIAEWSVAQREKKLAVLYCT"
     gene            complement(1858..3300)
                     /locus_tag="VNP73_10790"
     CDS             complement(1858..3300)
                     /locus_tag="VNP73_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWL66449.1"
                     /translation="MSGGDVFWKKTFKMVVFVTVVTSITLLGLVFIPAARAELGPATV
                     SARVHLGSGNTILRLPPLGALEASTHTAPLNATLSVEAVDIDRLATLATTAAGRAQLQ
                     QEIEDDLQGLVLAVAVRYGLGAALLGTLLGLVVFHRRWDRVLAVAVTALAAVAVPGVL
                     LARGYEPTAFDSPRYTGSLQRARAVMETLTAHIEVLDEARDRYALATRKLSDLFVLLS
                     HPEIGPTTAGTTVLHVSDIHANPLGLELTQELSREFDVAVVIDTGDISSAELDTGRIS
                     TVIDPVDAALAREVGRVGVPYVFTPGNHDSPQLRARLERTDDVHVLNGQSFEIAGISI
                     LGWADPTFSTTPVPEDEKAERRVAEAPEVGTAVEANDPDILAVHDVRLAEESFGDVPL
                     VLAGHTHERDLRQIDGTTVLTVGSTGATGLKSFTVEAERRYEAEVLYFEGAQLIAVDY
                     VSLADFGTDFRVDRRILTRRETDEDDRSGR"
     gene            complement(3467..>4051)
                     /locus_tag="VNP73_10795"
     CDS             complement(3467..>4051)
                     /locus_tag="VNP73_10795"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL66450.1"
                     /translation="GSLVYTWTGRDHLPYVARSTDGGKTWSPPMMIAPPKINEAALPR
                     VAAGPNGVIAVAYIGTENAPGGPPYYDFCNVHLGDCTDGRYAGVRWDGYIALIRHPDN
                     QDAVIRTTTAGRPSAPLLVGGCSAEGGCKAVLDFVDVQFDATGAPWAAFVDDCAIKRD
                     FEPVFSRDLPRCGDGVGEGIVLTIARVGRQGIEP"
BASE COUNT          883 a         1313 c         1222 g          633 t
ORIGIN      
        1 ggcgagaatg cgacggctgt gagcaacacg acagcagcga tcgaagcgga gatcgcgctt
       61 cgagcgggct ggaaagtact cacaccctag gcagttctgc gccggaaatg gtgactcctg
      121 ctcccagcgc atcgatcaca aacaaaagtt cccgacgacc cgcccggact aaaacgcttc
      181 ggaagaaact tcggcgaccg gatatatctg gcgccacgcg tcatatccac cggcaagagc
      241 acgagcctcc catcctgcct ttatcaatgt cagcgccgcc cgggcgctgc tgccctcact
      301 gggtcaggtg cagtacagca cggccaactt cttttcgcgc tgggccactg accactctgc
      361 gatgtcgtcg agcatcaccc tgatgctgcc ggggatggca ccagcgtctc gctggtattg
      421 cgagcgactc ctaacgtcca acacaagagg aggagaggca ccgcccagca atgtatgcag
      481 gtcctcgaca gtgatccgct cgacatcatc gacatgagaa acgaaaaggc ccgaagcggt
      541 gtcttcgcgc tcctcggcga gagtctccgc cgccaccctt cgggcgtacc aacctgtaac
      601 aggtgtcgcg gtgacgccat gagcgaggac ggaggcgatg acaacggcac cggtgaccgc
      661 gaacaacagc tcactaccgg gaaccccact caccacaaca agaagagcga acagcatcga
      721 atttaggcca cgcggaccga accaggcgat gaacgctcga gccggtgtgc tcaacgcaac
      781 accgcgcaag ctaagcagta ggccgagtgc cagcggtcgg gctacgacga tggtgaggat
      841 ggccagtgcc accgtctgcc acacgggtaa caggctgagc gaggcagaaa cggcgacccc
      901 gaacatcacg aacgccagca acatcgcgat ctccgccgcg gcttccccga actcgaggaa
      961 acaatcgcag agctcccgat cgagaatcgt cacggcgaat ccggccgcga acgcggcaag
     1021 gaagccgtcc cctcccacgg cgaccccgca ggcgtaagcc ccgagcacca agcccacgcc
     1081 gtagagggcc tgatactccc gacggatacc gaagtgggcg tctgcgcgcg acatgaggtc
     1141 cgacccgata gcgcctaccg cgaatcccgc cgccggcccc aacacaagca gctggagtgc
     1201 aaacagaaac cagtcacctg taccgccgac ctcccctctt gcgaccgcgg cgagcacgat
     1261 caccagcgga aggacaacaa cgtcattcgt ccccgcctcg accctgagcg cctgtcggac
     1321 cgaggctggt agacggcggt ccctgacgat gtctcgcagc acaacggggt ctgttgaaga
     1381 aagcaccgcg cccagcagga aagccgccac aggaggcata tcaaggagcc acatcccggc
     1441 ccccgccacg gtcgcaacga tcacaagtgt gcccggtccc aaagaaagcg cgggaaccat
     1501 ccattccttg cgctcccgtg cgaactcaag gttcaccgcg tcgaggaaca agacgagggc
     1561 cagagtcaat gtcgccacgg cctccagcga gggagagtgg acgtcgagat ccatcacctc
     1621 caagccgagc ggccccagca ggaaccccag accgagaaag atcatcggaa aagagatagg
     1681 tgcgcgttcc accacgcccg ctgcaagtgc cgagacagta agtacgacgg cgactagggc
     1741 gaagatcccc gctagttcca cggactagac cctaagacgg ggtgggaagg atcatctgca
     1801 gcccgatcct cctggagtgg gcattgcaaa gatcattacg gcttggctgg gcaggcctta
     1861 acggccggac cgatcgtctt cgtcagtctc gcggcgtgtc aagatccgac gatcgacccg
     1921 aaagtcggtg ccgaagtcgg caagactcac gtagtcgact gcgatgagct gcgccccttc
     1981 gaaatacaga acctccgcct cgtagcgccg ctcggcttcg acagtaaagg actttaatcc
     2041 ggtggctccc gtcgaaccga cggtcagcac ggtcgttccg tcgatctgac gcaagtcacg
     2101 ttcgtgcgta tggcccgcga gcacgagagg gacgtccccg aatgactcct cagcgaggcg
     2161 cacgtcgtga acggcaagta tgtcggggtc gttcgcttcc acggcggtcc cgacttcggg
     2221 tgcctcggct acccgtcgct cggccttctc atcctcgggc acgggcgtgg tggagaaagt
     2281 agggtcggcc cagccgagga tgcttatccc cgcgatctcg aagctctgcc cgttcagcac
     2341 atgcacgtca tctgtccgtt cgagacgcgc acgtaactgc ggtgaatcat gattccccgg
     2401 agtgaagaca tacgggactc cgacgcgtcc tacctctcgc gcaagggcgg catcgaccgg
     2461 gtcgatcacc gtggatatcc tgcctgtgtc aagttcggcc gacgagatgt cacctgtgtc
     2521 gatcacgacg gccacatcga actcccggga caattcctgc gtcagttcta ggcccagcgg
     2581 attcgcgtgg atgtcactga cgtggaggac cgtcgtcccg gcagtggtcg gaccgatctc
     2641 ggggtgggac agaagaacga atagatcaga caacttgcgc gtcgcaagtg cgtatcgatc
     2701 acgagcttcg tctagcacct cgatgtgtgc agtcaacgtt tccatgacgg cgcgagcccg
     2761 ctggagactt ccggtgtatc gcggcgagtc gaaagccgtc ggctcatagc cgcgcgcaag
     2821 aagaactccg ggcaccgcaa ccgccgccaa ggcagtaacg gccacggcga ggacacgatc
     2881 ccatcgccga tggaacacaa ccaagccgag caaggtaccg agtaacgcgg cgcccagccc
     2941 atagcggacg gcaaccgcga gaactaagcc ctgaaggtca tcctcgatct cctgttggag
     3001 ctgggcccga ccggcggcag tagtcgccaa ggtggcgagc ctgtctatgt cgactgcctc
     3061 aaccgagagc gtcgcgttca gtggagccgt gtgcgtcgag gcctccaacg cccccagcgg
     3121 tggcaaccgc aggatggtgt tcccgctgcc cagatgcaca cgggcggaaa ccgtggcggg
     3181 cccgagctct gctctggcag ccggtatgaa gacaagaccg aggagcgtga tcgatgtgac
     3241 gacggtgacg aagaccacca tcttgaaagt cttcttccaa aaaacgtccc ctccgctcat
     3301 ctcctcttcc taaccggccc acgggggatc cgttgctacc gatcgattgc aggtctggcg
     3361 gccggcgcgt ttcctggtag ggatggagac gaaggaggga ggccgaagtt tgcttgccca
     3421 gccgagcgga aagcaaagcg catcaagaac gacgatcagc gacggttcag ggttcgattc
     3481 cctggcgacc caccctggcg atcgtgagaa cgatcccttc gccaacaccg tcgccgcaac
     3541 ggggcagatc gcggctgaac acgggctcga agtcgcgctt gatcgcacaa tcgtcgacga
     3601 aggccgccca tggcgcaccg gtcgcgtcga actgaacgtc gacgaagtcg agcacagcct
     3661 tgcagccacc ctcggcactg caaccaccga ccagcaaagg cgcgctcggc cggccggcag
     3721 tcgtggttcg gataacggcg tcctggttat ccgggtgccg gatcagcgcg atgtacccgt
     3781 cccaccggac cccggcataa cgaccatcgg tgcaatctcc gaggtgcacg ttgcagaagt
     3841 cgtagtaggg aggacctccc ggtgcgttct cggtcccgat gtaagcaacc gcgatgaccc
     3901 cgttagggcc ggctgcaacg cgcggcaatg cggcctcatt gatctttggt ggagcgatca
     3961 tcatcggcgg gctccacgtc ttgccgccgt ctgtagagcg tgcgacgtat ggaaggtggt
     4021 cgcgaccggt ccacgtgtaa acgagcgagc c
//
LOCUS       DATMED010000413         3572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3018028, whole genome shotgun sequence.
ACCESSION   DATMED010000413 DATMED010000000
VERSION     DATMED010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018028"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VNP73_10800"
     CDS             complement(<1..926)
                     /locus_tag="VNP73_10800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWL66451.1"
                     /translation="MPDFKLPDLGEGVTEAEVDKWLVKEGDTIAEDDPLVEVITDKAT
                     AEIPSPFAGLVTKIHVMAGAIVPVGTVLVSIGSGAPESGPARTGGVSPGDDSKRSGGA
                     GGAPPGSGLGVKAMPPVRKLARELGIDIAKVEPSGTNGKVTREDVEAFASGGAVGTPS
                     SPQEGEKREPLRGVRRVIAERMSHAHRVIPPVTHVEECDVTELDATRRLANERAPDEP
                     RLTFLPFIVKAVVQGLKDHPALNASLDEDAGEIVFHDRYDIGVAVDTPAGLTVPVIRN
                     ADQKRLRQIAGEIERFATQARAGKLAAEDLRGG"
     gene            complement(929..1930)
                     /locus_tag="VNP73_10805"
     CDS             complement(929..1930)
                     /locus_tag="VNP73_10805"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWL66452.1"
                     /translation="MVDALNKGLHQAMDADDRVVVLGEDVARTGGVFRVTAGLLDRFG
                     EERVVDTPLAEAGIVGAAIGMAVYGMLPVVEIQFDVFVYPGFEQIVVHAARYAWRTKG
                     QVPASIVIRIPYGGGVRAPELHSDSPEALFAHVPGLKVVCPTTPTDARDMLVAAIADP
                     DPVIFMEPKRIYRAGREEVPEELPFSFGNGNGNGHPPPEILSKARIARDGNDLALITY
                     GATLPLSLEAADRLAGEGIETRVVDLRSLFPLDVDAITTAAAECGRALVVHEAPRNAG
                     LGAEVSSLIHERAMLDLEAPVGRVTGFDVPFPQFANEDDYLPSVERILAGARKVLDF"
     gene            complement(1942..3063)
                     /locus_tag="VNP73_10810"
     CDS             complement(1942..3063)
                     /locus_tag="VNP73_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWL66453.1"
                     /translation="MSEPFQIIGDGKDLPDRDVPDGLTDKDLIELFRWLMLLRTFDER
                     SVALQRQGRIGTYALYWGEEGTQAGPLYACNEGDWVFPSYRQNAIGILRGVPAATVLA
                     WWRGYGGEHGFYNPREHRVAPICVPIATHLPHAVGVSWAAKIRGDSVASIAWFGDGAT
                     SEGDFHEAMNFASVFKTATVFFCVNNQWAISTPLSRQTATKTIAEKAAAYAMPGVRVD
                     GFDPIACWKVTRDALERARAGDGPTLIEAFCYRLMAHGTADDPRLYRDEAVTEKWKPL
                     EPVGRTAAFLRRIGVLDDTKEEEIRAEAKQTLADAVAQMEAIEQPGAETIFEHVYASG
                     RPWSFDEGLEELRGVARQPEVKPLGPPTSDGAGEEGKTL"
     gene            complement(3070..>3572)
                     /locus_tag="VNP73_10815"
     CDS             complement(3070..>3572)
                     /locus_tag="VNP73_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66454.1"
                     /translation="SDNLAGLEGLLVGIIGLGAIGVSVAEIHKQEVDVFAPCALGGII
                     NDDTISEFRCKIIAGAANNQLARDELGDKLRDLGILYAPDFVINAGGLINVEDELRGY
                     DRTRALSRVEGIYKQLQLIFTMARERGISTAQAALEHAQERIRKISRIRLVRVTEFGP
                     LSTHHN"
BASE COUNT          601 a         1188 c         1124 g          659 t
ORIGIN      
        1 ccaccccgca ggtcttcggc cgccaacttc ccggcacgag cctgcgtagc gaaccgctcg
       61 atctcgccgg cgatctgtcg tagccgcttc tgatcggcgt tccggatcac gggaaccgtc
      121 agccccgcag gtgtgtcgac cgcaaccccg atgtcgtaac gatcgtggaa aacgatctcc
      181 cccgcatcct catcgagcga agcattgagc gcaggatggt ccttgagccc ctgaacaacg
      241 gccttaacga tgaatggcaa gaatgtaagc cgaggctcgt ccggagcccg ctcgttggca
      301 agccgccgag tcgcgtcgag ctcggtcacg tcgcactcct cgacatgagt aaccggagga
      361 atcacccgat gcgcatgcga catccgctcc gcgataacgc gccgcacccc ccgcaagggc
      421 tcccgttttt ccccttcctg aggagaagag ggcgtcccga ccgctccgcc cgaagcgaac
      481 gcttcgacgt cttcgcgagt gaccttgccg ttggttcccg acggctcgac tttggcgata
      541 tcgatgccga gttcgcgagc caacttgcgt accggaggca tcgccttcac gccgagtccc
      601 gagccgggcg gggcgccacc cgctcctcca gagcgttttg agtcgtcgcc gggcgacacc
      661 ccgcccgttc gggccggtcc cgactccggc gcccccgaac cgatcgaaac cagcacggtc
      721 ccaacgggga ctatggcgcc ggccattacg tggatcttgg tgacgagacc ggcgaacggc
      781 gacgggatct ctgcggtggc tttgtcggtg atgacctcga cgagcggatc gtcctcggcg
      841 atcgtgtcgc cctctttgac gagccacttg tcgacctctg cttcggtcac gccttcgccc
      901 aggtcgggca atttgaagtc gggcatggct agaagtcgag gaccttgcgg gcgcccgcaa
      961 ggatccgctc caccgatggc aggtagtcgt cttcgttcgc gaactgcggg aacggaacgt
     1021 cgaagcccgt gacgcggcca acgggggcct ccaggtcgag catggctcgc tcgtggatca
     1081 ggctggagac ctcggccccc agtcctgcgt tgcgcggggc ctcatgcacg acgagtgctc
     1141 gtccgcactc ggccgcggcc gttgtgatcg catcaacgtc caaggggaag agcgagcgga
     1201 ggtcgacgac tcgcgtttcg atcccctcac cggcaagtcg atcggcagct tcgagactga
     1261 gaggcagggt cgctccgtag gtgatcaggg cgagatcgtt cccgtctcgg gcgatgcgag
     1321 ctttgctcag gatctcgggc ggcgggtgcc cgttcccgtt cccgttaccg aacgagaagg
     1381 gcagctcctc cggaacctct tcgcgcccgg ctctgtagat cctcttcggc tccatgaaga
     1441 tgacgggatc gggatcggcg attgccgcga cgagcatgtc gcgcgcatcc gtcggagtgg
     1501 tgggacagac gaccttcaag ccgggcacgt gcgcgaacag tgcctcggga gagtcggagt
     1561 gcaactcggg agctcggaca ccgccaccgt aggggatccg gataacgatc gaggcgggta
     1621 cctggccctt ggtgcgccag gcatacctcg ctgcatgcac cacgatctgc tcgaagcccg
     1681 ggtagacgaa tacgtcgaac tggatctcga ccaccggcag catcccgtag acggccatcc
     1741 cgatcgccgc gccgacgatc ccggcttcag ctaggggcgt atcaaccacg cgctcttcgc
     1801 cgaaacgatc gagcaatccc gcggtgaccc ggaacacccc acccgtgcga gcgacgtctt
     1861 cgccgagcac gaccacgcgg tcgtcggcgt ccatggcctg atgcaagcct ttgttgagcg
     1921 cgtccaccat gttgagcgag gtcacagcgt ctttccctcc tcgccggcgc cgtcggacgt
     1981 gggggggccg agcggcttga cctcgggttg tcgcgcaacc ccacgcaact cttccaggcc
     2041 ctcgtcgaag ctccacggcc tgccggacgc atagacgtgt tcgaagatgg tttccgcgcc
     2101 cggttgctcg atcgcttcca tctgcgccac cgcgtcggcc agggtctgct tcgcctccgc
     2161 tcggatctct tcctctttcg tgtcatccag cacaccgatc cggcgaagga acgcggctgt
     2221 tctgccgacc ggctcgagcg gcttccactt ctcggtcacg gcttcgtcgc ggtagaggcg
     2281 tgggtcgtcg gccgtgccat gtgccatgag gcggtaacag aaggcttcga tcaaggtcgg
     2341 tccgtccccc gctcgggcac gttcgagcgc gtcacgcgtc accttccagc atgcgatcgg
     2401 atcgaatccg tcgacgcgga cgcccggcat cgcatacgcg gcagctttct cggcgatcgt
     2461 ctttgtagcc gtctgccgtg acaacggggt cgagatcgcc cactggttgt tgacacagaa
     2521 gaagaccgtc gccgtcttga agacggaggc gaagttcatc gcttcgtgga agtcaccttc
     2581 cgacgtagcg ccgtccccga accacgcgat cgaggcgacg ctgtcacccc ggatcttcgc
     2641 ggcccacgac acacccaccg catgtgggag gtgggtggcg atcggcacgc agatcggagc
     2701 gactcggtgt tcgcgggggt tatagaagcc gtgttcgccg ccgtagcccc gccaccaggc
     2761 aagcaccgtt gcggccggca cgcccctcag tatcccgatc gcgttctggc ggtacgaagg
     2821 gaacacccaa tcgccctcgt tgcatgcgta cagaggcccg gcctgggttc cctcctcgcc
     2881 ccagtagagc gcgtaggtcc cgatgcggcc ttgtcgttga agcgcgaccg agcgttcgtc
     2941 gaacgtccgc agcagcatga gccagcggaa cagctcgatc agatctttat cggtcagacc
     3001 atcgggcacg tcgcggtcgg gcaggtcctt gccgtcgccg atgatctgga agggttcgct
     3061 cacgatgatc tagttgtggt gagtggagag cgggccgaat tcggtcaccc gcaccagcct
     3121 tatgcgggag atcttacgga tccgctcttg tgcgtgttcg agagccgcct gcgcggtcga
     3181 tattccccgc tcgcgagcca tcgtgaagat gagctggagc tgcttgtaga taccttcgac
     3241 gcgtgagagg gccctggttc ggtcgtagcc gcgcagttcg tcctcgacat tgatcaatcc
     3301 tccggcattg atcacgaagt cgggtgcgta aaggatgccg aggtcgcgca gcttgtcccc
     3361 cagctcatcc cgtgccagct ggttgttggc cgcccccgcg atgatcttgc accggaactc
     3421 ggagatcgtg tcgtcgttga tgatcccgcc gagggcgcag ggagcgaaaa cgtctacctc
     3481 ctgcttgtgg atctcggcga ccgatacgcc gatggcgccc aatccgatga tgccgaccag
     3541 cagaccttcg aggccggcga gattgtcgct ca
//
LOCUS       DATMED010000414         7474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1478481, whole genome shotgun sequence.
ACCESSION   DATMED010000414 DATMED010000000
VERSION     DATMED010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478481"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VNP73_10820"
     CDS             complement(<1..1499)
                     /locus_tag="VNP73_10820"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66455.1"
                     /translation="MSSSLSAPTEPQNKRATASGVAQNKPVAAVIPLVPAWRVDHTFD
                     YAVPAELIDALEPGSLVRVPFGGRRVRAVVTTIEERTPDRALETIAGVVTAAPVAPPP
                     MVDVFDWIARRYVTPRGKAFARAVPSRVRVRVDALPPLPQDPSPDLVGSYAGGTEMIA
                     AIEKRDPDIWCFECLPTHDRSRLIAELVAAAGRGGDGTALVCVPEVRYGSLVLDGLDK
                     WMTAPARVDSAQSEADRSRSLLALAAGRGVGAGGRAALLAPARGLRLIVVDEEPHRSY
                     KEDRSPRYDARRLAIHRARLSGAVCVLMGTTLSVESGAAARTGEFGWVTPGRRERRDA
                     RPVVEFAERDEGSSLSRLLHGRIKDVLASGGRTALLAPAAGYSRAVWCVQCRRSLRCD
                     VCEAGMFFDRATGRRLSRLRCPRCSNTESPPEACPNCGGTDFRWLGAGSERLAEQLQK
                     AFPRALVVRADPDDLEASTERALQADIYVTTWIGTKASLRPEVSLVGVI"
     gene            complement(1471..2667)
                     /gene="metK"
                     /locus_tag="VNP73_10825"
     CDS             complement(1471..2667)
                     /gene="metK"
                     /locus_tag="VNP73_10825"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWL66456.1"
                     /translation="MSDISLFTSESVTEGHPDKMADQISDAILDEILADDPFGRVACE
                     TLITTGLVVVAGEITTSTYVDIPAVVRRTVTDIGYTSSKIGFDGETCGVSVSIQEQSP
                     DISAGVEHALEERTGEVSDSALDTQGAGDQGMMFGYACRDNEDLMPMPIWLAHRFAHR
                     LSEVRKAGVLPYLRPDGKVQVTIGYEGGKPKKLETLIISTQHQPNVDIETLLKPDLLQ
                     HVVDPLIPPMLDTDQVKVIVNPTGVFEIGGPRADTGLTGRKIIIDSYGGMARHGGGAF
                     SGKDPTKVDRSAAYAARYVAKNVVAAGLADRFEIQIAYAIGVAHPVSVFVEAFGTEKV
                     ATARIEKAILELFDLRPAAIIRDLDLRRPIYQHTAAYGHFGRTDKDFTWERTDKADEL
                     LSLCSD"
     gene            complement(2742..2882)
                     /locus_tag="VNP73_10830"
     CDS             complement(2742..2882)
                     /locus_tag="VNP73_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66457.1"
                     /translation="MKRLVAALTLAVVMAVGVAPVAHAGGKCAPGQHGNPHPAFKPGS
                     CK"
     gene            complement(3149..4402)
                     /gene="coaBC"
                     /locus_tag="VNP73_10835"
     CDS             complement(3149..4402)
                     /gene="coaBC"
                     /locus_tag="VNP73_10835"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWL66458.1"
                     /translation="MDTGASGSPPSGLAEVVAGKKIILCVTGGIAAYKVAFVARGLAQ
                     AGADVRVVMTRSAERFVGEQTFTALTGNHVYRELFGQGPDIPHIELARGADLVVVAPA
                     TANAMAKIAGGFADDLFSATVLTARCPILLAPAMHTEMWENAATQANVAILQERGIQF
                     VGPVEGALSSGDEGIGRMVEPDEILIECSRVVARSEELSGRKFVVTAGGTQEPIDPVR
                     FIGNRSSGLMGMEIARAASARGAKVILVVGPTNLRPPPDVEVLRVKTAQEMRDEVFRA
                     ASDADVIVKAAAVADWQSDVHADHKLKKAKGPPDIKLVPTPDILAELGASPSARKPNG
                     ILVGFAAETEPDPGRLALVALHKKKSKGADLIVANDVSSPDSGFEVPTNRAVIAGPDG
                     TVDVGLVTKAALAEALIDRIALLLT"
     gene            complement(4415..4666)
                     /gene="rpoZ"
                     /locus_tag="VNP73_10840"
     CDS             complement(4415..4666)
                     /gene="rpoZ"
                     /locus_tag="VNP73_10840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWL66459.1"
                     /translation="MMIEPRIESLLEDVDSKYTLVLLAAKRARQINAYFTQLGEGLAE
                     FTPPQIPLNTDRAPKSLSIALQEIVEGKVSYERTADGIK"
     gene            complement(4663..5244)
                     /gene="gmk"
                     /locus_tag="VNP73_10845"
     CDS             complement(4663..5244)
                     /gene="gmk"
                     /locus_tag="VNP73_10845"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWL66460.1"
                     /translation="MAFNPGAGRLFVISGPSGAGKGTVIREVLRRKPGMVLSVSATTR
                     PARPHERNGVDYRFISVPQFLGMRDRGEFLESAEVYGNFYGTPRSPVDASIAGGKSVI
                     LELDIQGALSVKRGKPDAVLIFIEPPSLDELFLRLRGRGTEETENLSERMRAAYEELK
                     NKGIYDHIVINDDLGRATDELVRIIEEKERNAR"
     gene            complement(5267..5584)
                     /gene="mihF"
                     /locus_tag="VNP73_10850"
     CDS             complement(5267..5584)
                     /gene="mihF"
                     /locus_tag="VNP73_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HWL66461.1"
                     /translation="MPLPPPLTEEQRRQALEKAAIARRKRADLKEQLKSGRTSLSDLI
                     ARTQDEIVGKMKVSTVLESLPGVGRVRAKKIMERLDISESRRMRGLGAKQKENLLSEF
                     AGR"
     gene            complement(5704..6279)
                     /gene="pyrE"
                     /locus_tag="VNP73_10855"
     CDS             complement(5704..6279)
                     /gene="pyrE"
                     /locus_tag="VNP73_10855"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWL66462.1"
                     /translation="MNQTEILEVLSSTGAVLKGHFRLSSGRHSDYFVQKFRVFEHPRL
                     TQSLGDEIARKFEGTFDVVASPAVGAIVLGFATALAAGVRMVFAEREEGSLAFRRGFT
                     LAPHERTLVVEDVITTGGSAREVVDLVRSSGAVPVGIGALIERVDPARSADLGAPLRA
                     LVRLEVESWDEAACPLCSAGHPLDDPGSRRL"
     gene            complement(6276..6977)
                     /gene="pyrF"
                     /locus_tag="VNP73_10860"
     CDS             complement(6276..6977)
                     /gene="pyrF"
                     /locus_tag="VNP73_10860"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWL66463.1"
                     /translation="MSPDTKLCIALDGDVTDVRRLASSVCDEADILKIGLTAFVSGGA
                     DLVTEIAASAPVFLDLKLHDIPAQVEGAADAAAKLGVKYLTVHAAGGPEMIKAAVAAA
                     GESVDILAVTMLTSLDDPGAEQIGFRGSVGEAVVRLADIAVAAGAAGVVCSPREVTEL
                     RSRLGPLTGGGPLLVVPGIRAAGGVADDQRRTMSGREAARAGADVIVVGRPITAAADP
                     VAAARGLRDEISQTS"
     gene            complement(6985..>7474)
                     /locus_tag="VNP73_10865"
     CDS             complement(6985..>7474)
                     /locus_tag="VNP73_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWL66464.1"
                     /translation="ATAAVVGSVKKSLSRIPVFPKLSPNVTSLSAIAGAAIDAGADGL
                     SLVNTVFGMAIDVGTRRPKLAGTIGGLSGPAIHPIAVRAIHEVHTNFPHVPIIGQGGV
                     ATADDALQMILAGASAVAVGTANFVNPKATIEIVEGIDAYVREQGATGVSELVGAVKI
                     EG"
BASE COUNT         1319 a         2481 c         2311 g         1363 t
ORIGIN      
        1 tcgataaccc cgaccaacga gacctccggc cgcaggctcg ccttcgtccc gatccacgtc
       61 gttacgtaga tgtcggcctg gagggcccgc tccgtggaag cctcgaggtc gtcggggtcg
      121 gccctgacga ccagcgcgcg gggaaaggcc ttctggagtt gctcggcgag ccgctcgcta
      181 ccggctccga gccagcggaa gtcggtcccg ccgcagttgg ggcatgcctc cggcggactt
      241 tctgtgttgc tacaacgcgg acatctaagg cggctcagtc tccttccggt cgctcgatcg
      301 aagaacatcc ccgcctcgca cacgtcgcag cggagagatc gccggcactg cacgcaccag
      361 accgcgcgcg agtagcccgc ggctggggca agcaaggccg ttcgcccacc acttgccagc
      421 acatccttga tccggccgtg gagcagtcgg gaaagggaac tgccttcgtc gcgctcggcg
      481 aattcgacga ccggcctggc gtcacggcgc tccctgcgtc ccggggtgac ccatccgaac
      541 tcccccgtgc gagcggccgc tcccgactcg accgagaggg tcgtccccat caacacgcac
      601 accgcgcccg agagccgcgc tctatggatc gcgagccggc gagcgtcgta tcggggtgag
      661 cgatcttcct tgtaggaccg atgaggttcc tcgtcgacca cgatcaggcg gagccctcgg
      721 gccggcgcca ggagagctgc tcggcccccg gcccccaccc ctcgtccggc cgccaaggcg
      781 agcagggatc ggcttcgatc cgcctcggac tgtgccgagt ccacgcgtgc cggagcggtc
      841 atccacttgt ccaatccatc gagcacgagc gacccataac gcacttcggg aacgcatacc
      901 aaggcggtgc cgtcgccccc tctgccggcc gctgccacga gctctgctat gagcctgctg
      961 cggtcgtggg tcggaagaca ctcgaaacac cagatatccg ggtccctctt ctcgatggcc
     1021 gcgatcatct ccgtgccgcc ggcgtaggaa cccacgaggt cgggagaagg atcctgcgga
     1081 agaggtggca acgcatcgac ccgcacgcgc acacgtgagg gaaccgcacg cgcgaacgcc
     1141 ttgcccctgg gagtgacgta gcgacgagcg atccagtcga acacgtcgac catcggtgga
     1201 ggcgcaaccg gagctgccgt aaccacacct gcgatcgtct ccagggcgcg gtcgggcgtt
     1261 cgctcctcga tcgtggtcac aacggctcga acccgccggc ccccgaaggg aacgcgcacg
     1321 agggagccgg gctcgagagc atcgatcagc tcggcaggaa cggcatagtc gaaggtgtga
     1381 tcgacccgcc acgcgggaac gagaggtatg acggccgcta ccggtttgtt ctgcgcgacg
     1441 cccgaggccg tcgcgcgttt gttctggggt tcagtcggag cagagagaga ggagctcatc
     1501 ggctttatcg gtgcgctccc aggtgaagtc cttgtctgtc cgtccgaagt gcccgtaggc
     1561 ggcggtgtgc tggtagatgg gtctgcgcag gtccaggtcc cggatgatcg ctgcggggcg
     1621 aagatcgaac aactcgagga tcgccttctc gatgcgcgcc gttgcaactt tctcggttcc
     1681 gaacgcctcg acgaagaccg acaccggatg tgcgacaccg atcgcatacg cgatctggat
     1741 ctcgaaacga tcggccaacc cggccgccac gacgttcttg gcgacgtatc gagcggcata
     1801 tgcggccgat ctatccacct tcgtcggatc cttgcccgag aacgcccccc ctccgtgcct
     1861 cgccatgccc ccataggagt cgatgatgat cttcctaccc gtcagcccgg tatcggcccg
     1921 ggggcccccg atctcgaaca cgcccgtcgg attcacgatg actttcacct gatcggtatc
     1981 gagcatcgga gggatcaagg ggtccacgac gtgttgcagc aggtccggct tcaacaatgt
     2041 ctcgatgtcg acgttcggct ggtgctgggt cgagatgatg agagtctcca gcttcttggg
     2101 cttgccgccc tcgtaaccga tcgtgacctg aaccttgccg tcgggacgta ggtacgggag
     2161 aacgcccgcc ttgcgcacct cggagagtct gtgagcgaac ctgtgtgcca gccagatcgg
     2221 catcggcatg agatcttcgt tgtccctaca cgcgtagccg aacatcatcc cttggtcccc
     2281 cgcgccttgg gtgtccagag cggagtccga cacctcgccg gtccgctcct cgagagcatg
     2341 ttcgaccccg gccgagatgt ccggtgattg ctcttggatc gagacgctga ctccacaggt
     2401 ttcgccgtcg aaaccgatct tggatgacgt gtagccgatg tccgtgacgg tgcgacggac
     2461 cactgcaggg atgtccacgt atgtggatgt cgtgatctca ccagccacaa caacgaggcc
     2521 ggtcgtgatc agtgtctcgc aggcaacgcg accgaacgga tcgtcggcca ggatctcgtc
     2581 gaggatcgcg tccgagatct ggtccgccat cttgtcggga tgaccttcgg ttaccgactc
     2641 agaagtgaac aagctgatgt ccgacatggc ggggagtcta gtgcggcata agcaaggggg
     2701 gcgcccacga aaaggcgccc cccttcttgc agttgttact gctacttaca cgagcccggc
     2761 ttgaacgcgg ggtgtgggtt cccgtgctgg cccggcgcac acttcccgcc ggcgtgcgcc
     2821 acaggcgcga ctccgaccgc cattacgacc gccaaagtga gcgcggctac caatcgcttc
     2881 atgccctctc ctttcaccta ctccgagcgc cgaaggtctc gacgttcggc gacgaatcag
     2941 cgtccggcag ccgccagagc accggacaac atggctccgg caaggcacag ggcgatcaag
     3001 gccatcgtga tgcggtgctt catcaccctc ctccgttacc gggtcctctc ccagttacag
     3061 gaagattctg tcaataccta ggccacgggg caaggacaag ggaggactat ttcgaaagat
     3121 ggcccgcgat caacgatgcc ctctgccgtc aggtgaggag caaggcgatg cggtcgatca
     3181 gcgcttcggc cagggccgcc ttggtcacga gccccacgtc gaccgtcccg tcggggccgg
     3241 cgatcaccgc cctgttggtc ggcacctcga agccagaatc ggggctcgag acgtcgttgg
     3301 cgacgatgag gtcggcgccc ttgctcttct tcttgtggag agcaaccagc gcgagccggc
     3361 cgggatcggg ttccgtctcg gccgcgaacc ccacaaggat cccgttcggt ttgcgagcgg
     3421 agggactcgc tccgagctcg gccaggatgt cgggggtggg caccagcttg atgtcgggtg
     3481 gtcctttcgc cttcttcagc ttgtgatcgg catggacgtc cgactgccag tccgcgaccg
     3541 cggccgcctt gacgatcaca tcggcatcgg aagccgcacg gaaaacctcg tcacgcatct
     3601 cctgagccgt cttcacgcgc aggacctcca cgtcgggagg cggacggaga ttggtggggc
     3661 cgaccacgag gatgaccttc gccccccgag cggaggcggc ccgagctatc tccatcccca
     3721 tgagtccaga ggaccggttg cctatgaaac gaaccggatc gatcggctcc tgagttcctc
     3781 cggccgtaac gacgaacttc ctgccggaca gctcttctga acgtgcgacg acgcgcgagc
     3841 actcgatcag gatctcgtcg ggctcgacca tcctgccgat gccctcatct ccggaggaca
     3901 atgccccctc aacggggccg acgaattgga tgcctcgctc ctggaggatg gctacattcg
     3961 cttgcgtagc cgcgttctcc cacatctcgg tgtgcatagc gggagcgaga aggatcggac
     4021 atcgagcggt caacacggtc gccgagaaga ggtcgtctgc aaaccctccg gctatcttcg
     4081 ccatcgcgtt cgcggtcgcc ggtgccacga caacgaggtc cgccccgcgc gcgagctcga
     4141 tatgtgggat gtcggggccc tgaccgaaga gttctctgta gacgtggtta cccgttaagg
     4201 cagtgaacgt ttgctcgccg acgaatcgtt cggccgatcg cgtcatcact accctgacgt
     4261 cggcccccgc ttgtgccagt cccctcgcga cgaaggcaac cttgtacgcg gcgatgccgc
     4321 ccgtgacaca caggatgatc ttcttacccg cgactacctc agccaggccg ctggggggtg
     4381 acccggatgc gccggtgtcc atggggagga ggtgttactt gatcccgtcg gcggtgcgct
     4441 cgtaggaaac cttgccctca actatctctt ggagcgcgat cgagagcgac ttgggagcgc
     4501 ggtcggtgtt caaggggatc tgcggcgggg tgaactcggc gagaccttcg ccgagttgcg
     4561 tgaagtaggc attgatctgc cgggcacgtt tggccgccag gaggacgagg gtgtacttcg
     4621 agtcaacgtc ctccaggagg gactcgatcc ggggttcgat catcatcggg cgttcctttc
     4681 cttttcctca attatacgaa cgagttcgtc ggtggctcta ccgaggtcgt cgttgatcac
     4741 gatgtggtcg tagatgccct tgttcttcag ttcttcgtag gcggcgcgca tcctctccga
     4801 gaggttctcg gtctcctcgg tcccccggcc cctgagcctc aagaaaagct cgtccagaga
     4861 ggggggttcg atgaatatca acaccgcatc gggctttcct cgcttgaccg agagagctcc
     4921 ctggatatcg agctcgagga tgacgctctt gccacctgct atcgaagcgt caacggggct
     4981 ccgcggcgtc ccatagaagt tgccgtagac ctccgccgac tcgaggaact cgccgcggtc
     5041 tcgcataccc agaaactgtg gaacagagat gaatctgtag tccacgccgt tccgctcgtg
     5101 agggcgagcc gggcgggtcg tggccgatac cgagagcacc atgccgggct tgcgcctgag
     5161 tacctctcgg ataacggttc ccttgccggc tccagacgga cccgatatga cgaagagccg
     5221 gcccgcgccg ggattgaaag ccatggggga agtttctttt agatccttat cttccggcga
     5281 attcgctgag gagattctcc ttctgtttcg cgcccagccc gcgcattcga cgggattcgg
     5341 agatatcgag tcgttccatg atcttcttcg cccgtacccg gcctacgccg ggcaacgact
     5401 cgaggaccgt cgaaaccttc atcttgccta cgatttcgtc ctgggttcgc gcgatcagat
     5461 cgctgaggct cgttcgaccg ctcttcagct gctccttgag gtcggccctc ttacggcgag
     5521 cgatcgcggc cttctcaagt gcctgtctgc gctgttcttc cgtaaggggt gggggcaagg
     5581 gcatccgagc ttccctcctt caagggccgt aagtgaagct ggcgatatgt ccgttatatc
     5641 tgcgccgagc cgtgccggat tataggcaag gcgagccagc gccgctcgcg accggcggcc
     5701 cgatcacaac ctccggcttc ccggatcgtc gagtggatgt cccgcggagc acaacgggca
     5761 ggccgcttcg tcccacgatt caacctcgag ccggaccagc gctctcaagg gagcgcccag
     5821 atcggccgag cgggccggat cgacccgttc tatcagcgcc ccgattccca cagggacggc
     5881 tcccgacgac cgcaccagat caaccacctc tcgggccgag ccgccggtgg tgatgacgtc
     5941 ctcgacgacg agcgttctct cgtggggcgc caaagtgaat ccccgacgaa aagccaggga
     6001 tccttcctca cgctcggcga agaccatccg taccccggcc gcaagggcgg tggcgaagcc
     6061 gagcacgatc gcacctaccg ccggactcgc cacgacgtcg aaggtgccct cgaacttgcg
     6121 tgcgatctcg tctccgaggc tttgagtgag tcgtgggtgc tcgaacacac ggaacttctg
     6181 aacgaaatag tccgaatgac gtcccgaaga cagccgaaaa tgacctttga ggaccgcccc
     6241 ggtgctggac aggacctcga ggatctcggt ttggttcacg aagtctggct gatctcgtcc
     6301 cgcagacccc gggcggcggc gaccggatcg gccgcagccg tgatcgggcg cccgacaacg
     6361 atgacgtcgg caccggcccg agccgcctcg cgaccggaca tcgttcgtcg ttgatcgtcg
     6421 gcaactcccc cggcggcgcg gatgccgggc acgaccagca gtggcccgcc gccggtgagg
     6481 gggcccaagc ggctcctgag ttcggtcacc tcgcggggcg agcagacgac gcccgccgct
     6541 cccgcggcca ccgcgatgtc ggccaaacgc accaccgctt ctcccacgga gccgcggaag
     6601 ccgatctgtt ctgcaccggg atcgtccaga ctcgtcagca tcgtcacggc gagtatgtcg
     6661 accgattccc ccgcggcagc gacggccgcc ttgatcatct ccgggcctcc cgccgcatga
     6721 acggtcagat acttcacccc cagcttcgcg gccgcatcgg ccgcgccctc gacctgcgca
     6781 gggatgtcgt gcaacttgag atccaggaac accggggccg aagcagcgat ctccgtcacc
     6841 agatcggccc cccccgacac gaacgcggtt agaccgatct tgaggatgtc tgcttcgtca
     6901 cagacactcg aagcgagtcg gcgaacatcg gttacgtcgc cgtctagagc gatgcatagt
     6961 ttcgtgtcgg ggctcatttg tcgctcatcc ttcgatcttc actgcgccca cgagctcgga
     7021 gactccggtc gctccctgct cgcgaacgta ggcatcgata ccctcgacga tctcgatcgt
     7081 ggccttcgga ttgacaaagt tcgcggttcc aactgctacc gccgacgcgc cggccaggat
     7141 catctgcaga gcatcgtccg ctgttgccac cccaccctgg ccgatgatcg gcacatgggg
     7201 gaagttcgtg tgaacctcgt gtatcgcacg caccgcgatc ggatggatcg ccggtcccga
     7261 caatcccccg atcgttcctg cgagcttcgg ccgccgcgtt ccgacgtcga tcgccatccc
     7321 gaagacggta ttcacgagtg agagcccgtc ggcacccgca tcgatcgcag cccccgctat
     7381 cgcgctgagc gaagtgacgt tcggcgacag tttcgggaag accgggatcc gagaaagaga
     7441 cttcttcacc gatccaacga cggctgcggt ggcc
//
LOCUS       DATMED010000415         3280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_709553, whole genome shotgun sequence.
ACCESSION   DATMED010000415 DATMED010000000
VERSION     DATMED010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709553"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VNP73_10870"
     CDS             <1..509
                     /locus_tag="VNP73_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HWL66465.1"
                     /translation="FSNPFSDIIFLIEKMNSVDVKPECECFDTGHIGNVEPLLDMGLL
                     KPPIQFSLILGVLGGAPATAKNLTHMAGILPEQSTWEVIAISRKQWHLVASAASLGGN
                     VRVGLEDNFYLPDGEMAGSNGDLVEAAASLIQLSGRQVAEPAEARRLLSLPERPDRTA
                     VLAEEAHA"
     gene            506..721
                     /locus_tag="VNP73_10875"
     CDS             506..721
                     /locus_tag="VNP73_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HWL66466.1"
                     /translation="MSEEVLAEMVANVWKVLVAPGDEVAEGDTICILESMKMEIPVEA
                     TAGGKVAEVNVAEGGVVQEGDVIAVID"
     gene            726..1508
                     /locus_tag="VNP73_10880"
     CDS             726..1508
                     /locus_tag="VNP73_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWL66467.1"
                     /translation="MTLVRVSREESVATLLLDRPDARNALSVQMCEDIVVALHEIGSD
                     PDARSCVIRGAGSVFCSGADFAAVSGASGVDFVPAFERMLESVARCSLPTVACIHGAA
                     LGGGLQLATVCDFRIVADDAKVGIPSAKLGILVNFENVQRLVLLAGIAVAKEVLMTAR
                     TYSGKEAAAVGLATRSVPADELEDVSYDYAEHVASLAPLSVRGTKRSIQVVVDHLASV
                     RGSHPDSVAAIDRLVVEAYQSEDLQEGIKAMSEKRSPRFTGR"
     gene            complement(1505..1873)
                     /locus_tag="VNP73_10885"
     CDS             complement(1505..1873)
                     /locus_tag="VNP73_10885"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HWL66468.1"
                     /translation="MEERLYTPDEATALLPNLAPTLVELREKFEAAARIRQTVAAAAS
                     SNGGSPERDAWERTLARVGELIERLQEWAVELRDLNQGLVDFPARIEDEEAWLCWRLG
                     EPEVAYWHPRTTGFADRRPL"
     gene            complement(1875..2561)
                     /locus_tag="VNP73_10890"
     CDS             complement(1875..2561)
                     /locus_tag="VNP73_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWL66469.1"
                     /translation="MRIAVISDIHGNLPALEAVAADVQTFEPDEIWCAGDIGFMGPWA
                     TDCIARVRDEGWTTVKGNTDVWITGDPQNVEDPGQRARFEELARAHAISSADAQWLLN
                     LPIGHSGRGSVLMVHGTPESPFIAPLPDAPPSEYEPYGGEASLVVFGHVHIAFTRRLT
                     DGTVVCNPGSVGLPMDGATASYVLIDQEGPNWTLRHRRVAFDRGRVIAAARAAGDPIE
                     SAFLGFFGTE"
     gene            2640..>3280
                     /locus_tag="VNP73_10895"
     CDS             2640..>3280
                     /locus_tag="VNP73_10895"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HWL66470.1"
                     /translation="MSTLAERIREVSDLSEREAQHLRMLCSSWQVLADLSFSDLLLYV
                     PTRTPDVYEICAQLRPFTSQTLYPNDMVRTRVTQPEQPVVERAYREGHTWSQEEPVLV
                     DGIPIRMDAVPVRFEGEVIAVLTKEGSPATSRRPGRLEQVYLDAAEIVSKMISEGLFP
                     FPGIPVGDWPRVGDGLYVLDGDGRITWASPNALSSLRRLGIKQNVLGRTLDELG"
BASE COUNT          577 a         1007 c         1059 g          637 t
ORIGIN      
        1 tcttctcgaa tccgttctcg gacatcatct ttctgatcga gaagatgaac agcgtcgatg
       61 tgaaacccga atgtgaatgc ttcgacacgg gccacatcgg aaacgtggag ccgttgttgg
      121 acatgggact gctcaaacct ccgatccagt tctcgctgat cctcggcgtt ctgggtgggg
      181 ctcccgcgac ggcgaagaac ctcacgcata tggccggcat ccttcccgag cagagcacgt
      241 gggaagtgat cgccatcagc cggaagcagt ggcacctcgt tgcatcggcg gcgtcgctgg
      301 gtggcaacgt gcgcgtcggt ctggaggaca acttctacct gcccgacggg gagatggccg
      361 gctcgaacgg cgatcttgtc gaagctgcgg ccagcttgat ccagctgtcc gggcgccagg
      421 ttgccgaacc ggccgaagcg aggcggttgc tgtctctacc ggagcgcccc gatcgcactg
      481 cggttcttgc agaagaagct cacgcgtgag cgaggaagtc cttgccgaga tggtggcgaa
      541 cgtgtggaag gtgctcgtcg cgcccggtga cgaggtggcc gagggcgaca cgatctgcat
      601 cctcgagtcc atgaagatgg agatcccggt cgaggcgacc gcaggaggca aggtggccga
      661 ggtcaacgtc gctgagggtg gggtggtgca ggagggcgac gtgatcgcgg tcatcgactg
      721 aacgggtgac cctcgtccga gtatcccgag aagagtcggt cgccacactt cttctcgacc
      781 gccccgacgc tcgcaacgcg ctatccgttc agatgtgcga ggacatcgtc gtggctctgc
      841 atgagatcgg ttccgatccc gacgcgcgct cgtgtgtgat caggggggcc gggtcggtct
      901 tctgttcggg tgcggacttc gccgccgtat ccggcgcgag tggggtcgat ttcgtgccgg
      961 ctttcgagcg gatgctcgaa tcggttgcgc gttgcagcct tccaacggtt gcgtgcatcc
     1021 acggcgccgc gctgggaggc ggcttgcaac tcgctaccgt ctgtgacttc cggatcgttg
     1081 ccgacgatgc caaggtcggc atcccttcgg ccaagctcgg catccttgtg aacttcgaga
     1141 acgtgcagcg cctggtgttg ctcgccggca tcgccgttgc gaaggaggtg ctgatgacgg
     1201 cacggaccta ctccggcaag gaggcggccg cggtgggcct agcaactcgg agcgtgccgg
     1261 cagacgagtt ggaggacgtg agttacgact atgccgagca cgtagcctcg ctcgcgccgc
     1321 tatccgttcg ggggaccaag agatcgatcc aggtggtggt cgatcatctt gcttcggtac
     1381 gaggttcaca tcccgatagc gtcgccgcga tcgatcgact cgtcgtagag gcgtaccaga
     1441 gtgaggatct gcaagagggc atcaaggcga tgtccgagaa gcgttcccct cgtttcaccg
     1501 gtcgctagag cggcctgcgg tcggcgaaac ccgtcgtccg cgggtgccag taggcgacct
     1561 ccggctcacc cagcctccag cacaaccatg cttcttcgtc ttcgatccgg gccgggaaat
     1621 cgacgagccc ctggtttaga tcccggagct ccactgccca ttcctgaagc ctctcgatca
     1681 attcaccgac acgggcaagc gtgcgttccc acgcgtcgcg ctcgggagat ccaccattag
     1741 acgacgcggc cgcggccacg gtctgccgga tccgcgccgc ggcctcgaac ttctcgcgca
     1801 gctcgaccaa ggttggtgcg aggttcggaa gtagagcggt cgcttcgtcg ggtgtgtaga
     1861 gacgctcctc cacgtcactc ggttccgaag aatccgagga aggccgactc gatcggatcg
     1921 ccggctgcgc gagccgctgc gatcacccgg ccgcggtcga aggcaacccg tcgatgtcga
     1981 agggtccagt tcgggccttc ctggtcgata agcacgtacg aagccgtagc cccgtccatc
     2041 ggaagcccga ccgagcccgg attgcaaacg acggtgccat ccgtgagccg gcgagtgaaa
     2101 gcgatgtgta cgtgaccgaa gaccaccaga cttgcttcgc ccccgtacgg ctcgtactcg
     2161 gatggaggcg cgtctggaag gggggcgatg aatggcgatt ccggcgttcc gtgcaccatc
     2221 agtaccgagc cacgaccact gtgaccgatc ggcaggttca acagccattg cgcgtccgcc
     2281 gaagagatcg cgtgagctcg agcaagctcc tcgaatctcg ctctttggcc gggatcctcc
     2341 acgttctgag gatcacccgt tatccagaca tcggtgttgc ccttaaccgt cgtccaacct
     2401 tcgtcgcgca cgcgggcgat gcaatcggtg gcccacggcc ccatgaatcc gatatcgccc
     2461 gcgcaccaga tctcgtcggg ttcgaacgtc tgtacgtcgg ccgcgaccgc ctcgagcgca
     2521 ggcaggttgc cgtggatgtc tgagatgact gctatccgca ttccgcttcc tcattaccct
     2581 ttttggagcg ggactctcat aacatctgta caaaggtcct gccacgctga aggaatctca
     2641 tgtcgacact ggccgagagg atccgtgagg tctcggatct ctccgaacgc gaagcccagc
     2701 atctgcggat gttgtgctct tcctggcagg tcctggcgga cctgtcgttc tcggatcttc
     2761 tcctctacgt gccgacccgt acgcccgacg tgtacgagat ctgtgctcag ttgcgcccgt
     2821 tcacttcgca aaccctgtat cccaacgaca tggtgaggac acgagtcacc cagccggagc
     2881 agcccgtggt cgagcgcgct tacagggagg gccatacctg gtcgcaagag gaacctgttc
     2941 tcgtcgacgg gatccccatc cggatggatg cggttccggt gcgattcgag ggagaggtca
     3001 tcgcggtttt gacgaaggaa gggagcccgg cgacctcgcg gcggccgggc aggttagagc
     3061 aggtctacct cgacgcggca gagatcgttt cgaagatgat ctccgagggt ctcttcccat
     3121 ttcccggcat ccccgtcgga gactggccgc gcgtcggtga cgggttgtac gtgctggatg
     3181 gtgacggacg gatcacgtgg gcgtctccca acgcgttgtc ctccctgagg cggcttggga
     3241 tcaaacagaa cgttctcggc cgcactttgg acgagctcgg
//
LOCUS       DATMED010000416         3300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4936508, whole genome shotgun sequence.
ACCESSION   DATMED010000416 DATMED010000000
VERSION     DATMED010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4936508"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..533)
                     /locus_tag="VNP73_10900"
     CDS             complement(186..533)
                     /locus_tag="VNP73_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017913403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HWL66471.1"
                     /translation="MTYEPGTTIPPRPPQEGQETYAGLGFAIASLVLGIVGLTAIPVI
                     PSILAIVFGYKARGEMNRSPGGQQARGLATAGLILGWVAIILLVLVVLVGALFLIPAG
                     IATFGAGAPPLGG"
     gene            complement(555..1628)
                     /gene="thrC"
                     /locus_tag="VNP73_10905"
     CDS             complement(555..1628)
                     /gene="thrC"
                     /locus_tag="VNP73_10905"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWL66472.1"
                     /translation="MNAGPLTVAGKRGIIRRYFDHLPVSEATPIVTLDEGDTPLIYSE
                     RLSTELDAEVWLKYEGLNPTGSFKDRGMTVAISKALEAGSRVVVCASTGNTSASAAAY
                     AARAGMICAVLIPQGNVALGKLAQALIHGARVLEIEGNFDVALELIKEMAKSNPITIV
                     NSINPFRVEGQKTAAFEIVEALGKSPDIHAMPVGNAGNITAYWKGYKQAQELGWTRDL
                     PKMWGFQAEGANPIVRGAPIDDPKTIATAIRIGNPASWSGATDAASESGGTIVSVRDK
                     EILEAYRSLAAEGVFVELASAASLAGLRQMKESGALQPGMTVTCVLTGHGLKDPDWAI
                     AGAPRPKLVAAKVDPILKELDLS"
     gene            complement(1625..2947)
                     /locus_tag="VNP73_10910"
     CDS             complement(1625..2947)
                     /locus_tag="VNP73_10910"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWL66473.1"
                     /translation="MSDRETPIRVGLLGFGIVGGATARILRDNAGEIARRTGARIELG
                     PVAVRSLSKKREIELPESQLTTDPWSVVTDPDVDIVVEAIGGIEPARDLILEAIKNHK
                     HVVTANKELLSTLGQDVMQAADIAGVDLLFEAAVGGGIPIIRPMKESLSGDRVRRVMG
                     ILNGTTNYILTRMSETGESFAEALQEAEGLGYTELDPSADIEGFDAAQKLAILSSIAF
                     NSRVRAGDVHREGISKVTASDIAAAHDLGYEVKLLAVAELDEGEVAARVHTAMIPLSH
                     PLASVRDVFNAVFVEAEEVGELMFLGRGAGGGPTGSAVVGDVIEIARNIMTGGRSPGC
                     TCYEDSKIRPQDQTHVRYYIVLSVLDQSGVLANVAGAFARHDVSIASVRQEGTGDEAT
                     LVLITHTATEGQHQATFAELGSLDVVKDIRSTMRVEGAAEDSGATSSR"
     gene            complement(3007..>3300)
                     /locus_tag="VNP73_10915"
     CDS             complement(3007..>3300)
                     /locus_tag="VNP73_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66474.1"
                     /translation="LLLAQLAENPALDPLFQDLFDSGRAEMVLKPASAYVPLDQPLPF
                     GAAVRSGALRGEVVIGYRAGRNGRAVSDVVVNPTKDLKVTFGETDQLIILTSD"
BASE COUNT          587 a         1098 c         1012 g          603 t
ORIGIN      
        1 cgtgtcgtcc tcgggaagtg tctcggcccc ctcgccttcg accatgatct cgatggagtc
       61 gcgacgaggc tcgatctcga cctcgagatg catccggatc gcagcgccga gcgcgtcgaa
      121 acccggacct agattcgcaa cggtggcggg aaccacaacc ttcactgcag cgggcacgtg
      181 tcctcctatc ctccgagcgg cggcgcgccg gcaccgaaag tggcgatccc tgccgggatc
      241 aagaacaaag cgccgacgag aaccaccaaa accagaagga tgatcgcgac ccatcccagg
      301 atcagcccgg ccgtagcgag gccccgggcc tgctgccccc cgggcgatcg gttcatctcg
      361 ccgcgggcct tgtagccgaa cacgatggca aggatggagg ggatcacggg gatcgcggtc
      421 aggccgacga tcccaaggac caacgaggcg atcgcgaacc cgaggccggc gtaggtctcc
      481 tggccctctt gggggggccg gggcgggatg gtcgttccgg gctcgtatgt catacgcagc
      541 acgatagtcc gtgcctagct caggtcgagt tctttgagga tcggatccac ctttgcggcc
      601 accagcttcg ggcgaggagc gccggctatc gcccagtccg ggtccttcaa cccgtggccc
      661 gtcaagacgc aggtgaccgt catccccggc tgcagcgcac cggactcctt catctgacgg
      721 agcccggcca gacttgccgc cgaagccagt tctacgaaga ccccctcggc ggccagagat
      781 cggtaggcct cgaggatctc tttgtcgcga acggaaacga tcgttccccc cgactcggat
      841 gccgcatcgg tggccccgga ccacgatgcc ggattaccga tccggatcgc ggttgcgatc
      901 gtcttggggt catcgatggg agcaccgcgc acgatcggat tggcaccttc cgcttggaag
      961 ccccacatct tcgggagatc cctcgtccat ccgagttcct gtgcctgttt gtaacccttc
     1021 caataagcag tgatgttgcc cgcgttgccc accggcatcg cgtggatatc gggactctta
     1081 ccgagtgctt ccacgatctc gaacgcggcc gtcttctgac cctccacgcg gaacggattg
     1141 atcgaattga cgatcgttat cggattcgac ttggccatct ccttgatgag ctcgagagcg
     1201 acatcgaagt tcccctcgat ctcaagaacg cgcgcgccgt ggatcagtgc ttgagcgagc
     1261 ttcccgagag cgacgttgcc ctgagggatg agtacggcgc agatcatccc cgctcgcgcc
     1321 gcgtacgccg cggccgatgc actcgtgtta ccggtcgaag cgcacacgac gacacgcgag
     1381 cccgcttcta gtgccttcga gatcgcgacc gtcatcccac gatccttgaa ggaccccgtc
     1441 gggttgagcc cctcgtactt cagccagacc tccgcatcca actcggtgga gaggcgttcc
     1501 gaatagataa ggggcgtgtc accctcatcg agcgtgacga tcggcgtcgc ttccgagacc
     1561 ggtagatggt cgaagtagcg gcggatgatg cccctcttgc cggcgaccgt taacggtccg
     1621 gcgttcaccg cgatgaggtg gcccccgagt cctcggctgc accttccacc cgcatggtcg
     1681 acctgatgtc cttgacgaca tcgagcgagc cgagctcggc gaaggtcgct tgatgctggc
     1741 cttcggtcgc agtgtgcgtg atcagcacga gtgtcgcttc gtcccccgtc ccttcttgcc
     1801 gaacgctcgc tatcgagaca tcgtgccggg cgaaagcgcc cgcaacgttc gccagcacac
     1861 ccgactgatc caataccgac aggacgatgt agtagcgcac gtgtgtctga tcttggggcc
     1921 ggatcttgga gtcctcgtag caggtgcagc cgggagatct gcccccggtc atgatgttgc
     1981 gcgcgatctc gatcacgtcg cccacgaccg ccgacccggt aggcccgcca ccggcccccc
     2041 gcccgaggaa catgagctcg ccgacctcct cggcttctac gaaaacggca ttgaagacgt
     2101 ctcgaacgct tgcaagtggg tgggacagag ggatcatggc cgtgtgcacg cgtgccgcta
     2161 cctcgccctc gtctaactcg gccaccgcta agagcttcac ctcgtagccg aggtcgtggg
     2221 ccgccgcgat gtccgaggcc gtgaccttgg agatcccttc ccggtgaacg tcccccgcgc
     2281 gcacgcgaga gttgaaagcg atcgaagaca agatcgcgag tttctgcgct gcatcgaagc
     2341 cctcgatgtc ggcagatggg tcgagctccg tgtaaccgag cccctccgcc tcctgcaagg
     2401 cctctgcgaa cgactccccc gtttcggaca tgcgcgtcag gatgtagttg gtcgtcccat
     2461 tcaggatgcc catcactcgc cgaacccgat cgcccgagag ggactccttc atcggtcgga
     2521 tgatcgggat gccgcccccg accgcagcct cgaacagaag atccacaccg gctatgtccg
     2581 cggcctgcat gacgtcctgg ccgagcgtcg agagaagctc cttgttcgcc gtgaccacgt
     2641 gtttgtggtt cttgatcgcc tcgaggatga gatcccgagc gggctcgatc ccgccgatcg
     2701 cttccacaac gatgtccacg tcgggatcgg taacgaccga ccaggggtcg gtggtcagct
     2761 gcgactcggg aagctcgatc tcgcgcttct tcgagaggct gcgcaccgcc accgggccga
     2821 gctctatacg tgcgccggtt cgccgggcga tctcgcccgc gttgtcacgc agtatccggg
     2881 cggtcgcacc gcccacgatc ccgaagccga gcagtcctac ccggatcggg gtttcccggt
     2941 cgctcacatc cgctccttgg ggtggataga catagaaaag gtacgacctc tccgggcgtc
     3001 gccgggtcag tcggaggtca ggattatgag ctggtcggtc tcaccgaacg tcactttcag
     3061 gtccttggtc gggttcacga cgacgtccga gaccgccctt ccatttcggc cggctcgata
     3121 gccgatcacg acttcgcctc tgagtgcgcc cgagcggacg gcggctccga aaggcaaggg
     3181 ctggtcgaga ggtacgtaag ccgaagccgg tttgaggacc atctcggcgc gcccggagtc
     3241 gaacaggtcc tggaagagcg gatcgagcgc cggattctcc gccaattgag ccagaagcag
//
LOCUS       DATMED010000417         5741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3786591, whole genome shotgun sequence.
ACCESSION   DATMED010000417 DATMED010000000
VERSION     DATMED010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3786591"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VNP73_10920"
     CDS             <1..235
                     /locus_tag="VNP73_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66475.1"
                     /translation="SDSYQELSELQAAAVAETDPDQRLEILGQIETLIVEEGPIIPLL
                     MIDQNHAARGEIEGLYSHSIARLDLTHITSGTR"
     gene            262..1323
                     /locus_tag="VNP73_10925"
     CDS             262..1323
                     /locus_tag="VNP73_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL66476.1"
                     /translation="MSAPGIFARTAPVITYAARRLALVPIVLFGMLLLVFTVSQLVPV
                     DPVAYFLGSRQEALGPQSEAVVEALERRWGWDKPIYERFVIYVSNIVQGDLGESSSTR
                     RPVIDDLIQFVRATLELIVFTLIVALAIAIPLGIVAAHRRGRIVDTLFNVITVVGVST
                     PSFWLALLSLNLFYGQLGWAAGAGRLDVFLVEPPRVTGFIVIDSILAGRLDALVNALH
                     HLALPVGILGLVIGVYYARVVRTEMIEALDSDFTRTAIGKGLSDRAVLYGHALRNALT
                     STITLTGLSFGSLMTGAIVVEAITGWPGLGSYTFRVTTRLDLPAIAGVALTVGLIYML
                     LNLIVDLLYAVADPRVRVR"
     gene            1323..2201
                     /locus_tag="VNP73_10930"
     CDS             1323..2201
                     /locus_tag="VNP73_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL66477.1"
                     /translation="MEVAVPPEVREAGRATQILRRLQARQFTLLEIGGLVVVALVLLA
                     VAAASIISPIDPAAQDFNSRLLPPSADHPFGTDQLGRDQLSRVLHGGRVSIVMGVLII
                     AIGGTMGLIAGATAGFLGGIIDEAIMRIADVFLSFPSIMLALVVAASLGPNLQNAAIA
                     IAVAWWPFYARIVRGQVISVRDRDFVTATRTLGASEARILLRTVLPNSLGVIRLVFVL
                     DIGFAIIAGSTLSFLGLGVSPPTPEWGLMIREAMMHSEAWWMMVFPGLAVVMLVSAIN
                     FAGGVLTRTGAEAAVR"
     gene            2198..3190
                     /locus_tag="VNP73_10935"
     CDS             2198..3190
                     /locus_tag="VNP73_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL66478.1"
                     /translation="MTTDAPPLLDVRDLRITFSLPGRLIHAVRGITFEVRGGERIGVV
                     GESGSGKSATALAIMRLLPRSTAVTGQILLDGVDLLQLSSKEIRTIRGRAIGIVYQDP
                     FTALNPVLTVGQQVAEVISRHFGTPRQAARREAANLLLRVGISSAADRLDDYPHQFSG
                     GMRQRVMIAIALAGRPRLIIADEPTTALDVTIQAQILELLLTLTQEDNTALLLITHDL
                     AVIARAIERVNVMYAGHIVEQADTRTVFKRPNHPYTNALLSSLPRVDDPSSSFTAIAG
                     QPPDPSRPDTGCPYVDRCDRPLSYCADRFPGLDRTDPYGSSANRVACWNPVNTQ"
     gene            3163..4245
                     /locus_tag="VNP73_10940"
     CDS             3163..4245
                     /locus_tag="VNP73_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWL66479.1"
                     /translation="MLEPRQHPMSLALSEPRAGPLLQVNDISVTFHANGLFRRSRPVR
                     AVAGVSLDVRRGETVALVGESGSGKTTTGRAILRLVPLSGGSIEFDGHDLAAARRRDL
                     PMIRRKVQMVFQDPYASLNPRLTVEQLVREPLDIHGIGSKSVRQERVATTLEQVGIEW
                     RDRGRRPRHFSGGQRQRIAIARAIALDPQLIVADEPISALDVSIQAQILSLLEQLQRD
                     RGIAYLLIAHDLAVVRHVSKRVAVMYLGKIVEEGPTHEVFTSARHPYTTALLSAAPVP
                     DPDVEATRRRIILRGDVPSPANPPSGCRFHTRCWLSESLGNPERCRTEEPSLEAATEG
                     ASGHRVACHFSEATSSGATPNAHLAP"
     gene            4267..5439
                     /locus_tag="VNP73_10945"
     CDS             4267..5439
                     /locus_tag="VNP73_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66480.1"
                     /translation="MSGRSSSTGNSADRCCGGIGRAGAGGPEVRTVLGSIPTSAVVGP
                     VMMHEHLLWDARGQWESIGGNPTKRLALEHGRVPFRADLAGPARFNVLSYRENWANSG
                     ATGLQRAVAEVAYLLEAGGVMVVDLTPHGIGPDPGGLRRLSTTTGLHVVAGSGIYTHD
                     THESWMETATVEEFETFFRKEVSDGIGASAVRPGILGEMGTSARPRSCELRALRAAAR
                     VAAGSGLSLNIHCHPGSQRVTELLIDITTEEGLSPERTILSHLDEIASHAYLSSVLAR
                     GVTLCFDGFGHDHYAGSDAKRRSDRQKMRMLARLVNDGRAPQLTMSQDVCRLHMMRRF
                     GGGGYDHVLARVVPTLRGSFDLDDDALRQILETNPRRLLEISGPPNHTRQSPMART"
     gene            5436..>5741
                     /locus_tag="VNP73_10950"
     CDS             5436..>5741
                     /locus_tag="VNP73_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HWL66481.1"
                     /translation="MIDLQGMARDCTHRVTLTVALPREEAIELEVHVVRGATDGPTVG
                     AFAGIHGDEQVGVAALARLAETLGAEKIRGTMILMPIVNPPATWAGSRLIPNEGTYLN
                     "
BASE COUNT          990 a         1865 c         1742 g         1144 t
ORIGIN      
        1 gtccgactct tatcaggaac tctcggaact tcaggcggcc gcggtggcgg agactgaccc
       61 tgaccagcgc ctcgagattc tcggccagat tgagaccctg attgtcgagg aaggcccgat
      121 tattcctcta ctgatgatcg atcagaatca tgcggcgcga ggcgagattg agggtctgta
      181 ctcgcactcg atcgctcggc ttgatctgac gcatatcacg agtgggacac ggtaggatcg
      241 gccccagatg ccggaggcgg catgtccgcc cccggcatct tcgcccgcac cgcacccgtg
      301 attacctacg ccgcccggcg gctcgccctg gtgccgatcg tcttgttcgg catgctgctc
      361 cttgtgttca cggtctccca attggtcccc gtcgatccgg ttgcctactt ccttggatca
      421 cgtcaagagg cgctcggccc acagtccgag gccgtggtcg aggctctcga gcggcgatgg
      481 ggttgggaca agccgatcta cgagcgattc gtcatttacg tctcaaacat cgtgcagggt
      541 gatctcggtg aatcgagcag cactcggcgt cccgtcatcg acgacctcat ccagttcgtt
      601 cgcgccacgc tggaactaat cgtatttaca ctcatcgtcg ctctagcaat cgcgatccca
      661 ttgggcatag tcgccgccca tcgtcgtggc cgtatcgtcg atacgctctt taacgtcatc
      721 acggtcgttg gcgtgtcgac gccgtcgttc tggcttgcgc tactgtccct caatctcttc
      781 tacggacagc tcggttgggc ggcgggcgca ggtagactcg acgtcttcct cgttgaaccc
      841 ccccgcgtta ccggattcat cgtcattgac agcatcctcg cgggtcgcct tgatgcgctc
      901 gtcaacgcgc tccaccacct cgccctgccc gtggggatcc tcggactcgt catcggcgtt
      961 tactacgcgc gtgtcgtgcg caccgaaatg atcgaggctc ttgatagcga tttcacccgc
     1021 actgcgatcg ggaagggact ctcggatcgt gccgtccttt acggtcatgc gttgcgaaat
     1081 gcgctcacct cgacaatcac cctgacgggg ctgtcgttcg gctcgctcat gaccggagcc
     1141 atcgtcgtgg aagcgattac aggctggccg ggactaggga gctacacgtt tcgggtgaca
     1201 actcggcttg atcttccggc gatcgcaggc gtcgctctga ccgtcggcct catctatatg
     1261 ctgttgaacc ttatcgtgga ccttctgtat gcggttgccg acccgcgcgt gagggtccgt
     1321 tgatggaggt cgctgtgccg cccgaggtcc gcgaggctgg acgggccacg cagattctgc
     1381 ggcgtctgca agcgcgccag ttcacgttgc tcgagatagg cggcctggtg gtcgtggcgc
     1441 tggttcttct cgcggtggcg gcggcctcga taattagccc tatcgatccc gccgcacaag
     1501 acttcaacag ccggttgctt ccgccatcgg cggaccaccc attcgggacg gatcaactcg
     1561 ggcgcgatca gctttcacgg gtgttgcatg gtggacgagt gtccatcgtc atgggcgtcc
     1621 tcatcatcgc catcggagga acaatgggac tgatcgcggg agcaacggcc gggtttctcg
     1681 gaggcattat tgatgaagcc atcatgcgaa ttgctgacgt gttcctcagc ttcccgtcca
     1741 tcatgctggc gttagtcgta gcagcctcgc tcggcccgaa tttgcagaat gcggcgatcg
     1801 cgatagccgt cgcctggtgg ccgttctatg cgcgaatcgt gcgcggccag gtcatcagtg
     1861 tgcgcgatcg agactttgtc actgcgacgc ggacgctcgg cgcgagcgag gcgcggatcc
     1921 tgctccgaac cgttcttccc aatagccttg gggtcattcg gcttgtcttc gtcctcgaca
     1981 tcggcttcgc catcattgcc ggttcgacgc tgagcttctt gggactcggc gtctcgcctc
     2041 ccacgccgga gtggggcctg atgatcaggg aggccatgat gcattctgag gcatggtgga
     2101 tgatggtctt cccaggcctc gccgtggtta tgctcgtgtc cgccattaac ttcgctggcg
     2161 gggtgctgac ccggaccggc gccgaggcgg cagtccgatg accactgacg ccccgcccct
     2221 tctcgacgtc cgcgacctac gtattacctt cagcctgccg ggtaggctga tccatgcagt
     2281 tcgcggcatc acctttgagg tccggggcgg agagcggatc ggagtcgtcg gcgagagcgg
     2341 cagcggaaag agtgccacgg ctctggcgat tatgcggctc ctgccccgct cgaccgccgt
     2401 gaccggacag attctgctcg atggagtaga tctccttcag ttgtcgtcga aagagatccg
     2461 caccatccgg ggccgcgcaa ttgggatcgt ttatcaggat ccttttaccg ccctgaaccc
     2521 tgttctgacg gtgggccaac aggtcgccga agtcatctct cgccactttg ggacgccgcg
     2581 tcaggcagcg aggcgcgagg cggcaaatct cctactccgc gttggaatct cgagcgcggc
     2641 tgatcgtctc gatgactacc cccaccagtt cagcggtggc atgcgtcaaa gggtgatgat
     2701 cgctattgcg ttggcaggcc ggccacggct gatcatcgcc gatgagccta cgacggcgct
     2761 ggacgtgaca atccaagccc agattctcga gctcctgcta actctgaccc aggaggacaa
     2821 cacggcgctc ctcctcatca ctcacgacct ggctgtgatt gctcgcgcga tcgagcgggt
     2881 caatgtgatg tacgcaggtc atatcgtcga acaggccgac actcgcacgg tgttcaaacg
     2941 tccgaaccac ccatacacca atgcgctgct ctcgtcgttg ccgcgcgtcg acgatccgtc
     3001 gtcgtccttc accgcgattg ccgggcaacc gccggatccc tcacgacccg acacgggctg
     3061 cccatacgtt gaccgatgcg atcgccccct ttcctactgt gccgacaggt ttcctgggct
     3121 tgaccgcacg gatccctacg gctcgtccgc taaccgtgtg gcgtgctgga accccgtcaa
     3181 cacccaatga gcctcgctct ttccgagcct cgcgccggcc cccttctcca ggtcaatgac
     3241 atctccgtca cgttccacgc caacggactc ttccggcgaa gccggccggt tcgcgcggta
     3301 gcaggcgtat ccctcgacgt tcgacgcggg gaaacggtcg cgttggtcgg agaatccggt
     3361 agcggcaaga ccacgactgg tcgtgccatt ctgcgcctcg tccccctcag tggaggctcg
     3421 atcgagttcg atggccatga cctcgctgcc gcgcgacgcc gcgatctccc gatgattcgg
     3481 cgcaaggttc aaatggtgtt ccaagatcca tacgcgagcc tcaatcctcg gctaaccgtc
     3541 gaacagcttg ttcgtgaacc actggatatc cacggcatcg gcagtaaatc cgtccggcag
     3601 gagcgtgtcg ccacgacact cgagcaagtc gggatcgaat ggcgcgatcg cggccgacgt
     3661 ccgcggcact tcagcggggg tcagcgccag cggattgcaa tcgcccgggc aatagccctc
     3721 gacccgcagc taatcgtcgc ggacgagccg atcagtgccc tcgacgtcag cattcaggcc
     3781 cagatcctct ctctgctgga acaattgcaa cgggatcgcg ggatcgctta cctcctaatc
     3841 gcgcacgacc tcgccgtggt ccgccacgtc agcaagcgcg tcgccgttat gtacctcggc
     3901 aagatcgtcg aggaaggtcc gacccacgaa gtcttcacct ccgcgcgcca cccttacacg
     3961 actgcgctcc tgtcggcggc tccggtgccc gatccggacg tcgaagcgac ccgtcggcgc
     4021 atcatcctcc gtggagatgt gccgagcccg gccaatcctc cttcaggctg ccgctttcat
     4081 actcgatgct ggctcagcga aagcctggga aaccccgagc gctgtcgcac ggaggagccc
     4141 agtctcgagg ccgcgaccga aggcgcctcg gggcatcgag tcgcatgtca cttctcagag
     4201 gcgacatcgt ccggtgccac accgaacgct cacctggcac cctgaagacc cctacccaaa
     4261 ccagagatga gcggccgctc ctcgagcacg ggcaactctg cggatcgctg ctgcggcggc
     4321 attggtcgcg caggagcggg gggacccgag gtccgcacgg tgctgggctc gatcccgacc
     4381 agcgcagtcg tcggtccggt gatgatgcac gagcatctcc tctgggacgc gcgcggccag
     4441 tgggagtcca tcggagggaa tcctacgaag cgattggcat tggagcacgg gcgtgtccct
     4501 ttccgcgccg acctcgcggg gcctgcaagg ttcaacgttc tctcctaccg cgagaactgg
     4561 gcaaactctg gggcaacggg actgcaacgg gcagtggccg aagtcgcata cttgctagag
     4621 gcgggcgggg tgatggtcgt cgacctcacg ccccacggca ttggacccga tccggggggt
     4681 ctgcgccgac tctcgacgac taccggactc catgtagtcg ccggcagcgg catctacacc
     4741 cacgatacgc acgaatcctg gatggaaacg gccacggtcg aggagtttga gacgttcttt
     4801 cgaaaagaag tctccgacgg catcggcgcg tctgccgtgc ggccaggaat acttggggag
     4861 atgggtacat cagcccgtcc ccgctcatgc gagctccggg ccctccgggc cgcggctcga
     4921 gttgccgcag gttctggcct gtcgctcaac atccattgcc accctggcag tcaacgcgtc
     4981 acggagctgc tcattgacat cacgacagag gagggcttgt cgccagaacg cacgatcctc
     5041 tctcatctcg acgagatcgc atctcatgcc tacctgtcgt ccgtcctggc gcggggggta
     5101 accctgtgct tcgacggctt cggccatgac cactacgccg gctcggacgc caagcgacga
     5161 agcgatcgtc agaagatgcg catgctcgcc cgtctcgtca atgatggccg agcaccgcaa
     5221 ctgaccatgt cgcaggacgt gtgccgactt cacatgatgc gacgttttgg tggcggcgga
     5281 tacgaccatg tgcttgcccg ggtcgtccct acgctccggg gctccttcga tctcgatgac
     5341 gatgcgctgc gccagattct ggagacaaac ccccgtcgat tgttggagat ctcgggaccg
     5401 cctaaccata cgcgtcagtc gccgatggca cgaacgtgat cgacctgcag ggcatggctc
     5461 gggactgcac ccatcgggtg acgctgaccg ttgcgctacc cagagaggaa gccatcgagc
     5521 tcgaggtcca cgtggtgcgc ggcgcgacgg acgggccgac ggtcggtgcc ttcgcgggca
     5581 ttcacggcga cgagcaagtc ggggtggcgg ccctggcgcg actggccgag acgctgggtg
     5641 ccgagaaaat acgcgggacg atgatcctga tgccgatcgt taacccgccg gcgacttggg
     5701 ccggatcgcg tctcataccg aacgaaggaa cttacttgaa c
//
LOCUS       DATMED010000418         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5322269, whole genome shotgun sequence.
ACCESSION   DATMED010000418 DATMED010000000
VERSION     DATMED010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5322269"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VNP73_10955"
     CDS             complement(<1..272)
                     /locus_tag="VNP73_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66482.1"
                     /translation="MSGITSRGVLTKEAGSRSSSAVLLGGVLVGILQAFSPVAFWWLD
                     HDVVWAVSLVAIAFVYVGFAVADGRRSIIAIESVVAMGFVVLSCVA"
     gene            complement(269..1969)
                     /locus_tag="VNP73_10960"
     CDS             complement(269..1969)
                     /locus_tag="VNP73_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HWL66483.1"
                     /translation="MKQSDLFSTETTGLPQATRPIDIRLKEGDRLDLHIGSVRKMVGD
                     DEVRMLAYNGSIPGPTLIVDQDSKITVDVNNEGDVDATVHWHGLRLENRYDGVPGDTQ
                     APIEVGGTYTCRVHFPDAGFYWYHPHIREDYAQDMGLSGTIVVRPTDPSYWPAVDREL
                     TITLDDVLIEDGKMAPFRRSGPTFTAMGRFGNVMLINGETEFSDKAAAGEVVRLNLVN
                     TANTRIFNFGLRGARMKLVGGDSGRYEREVFVDEVMLSPSERAIVDVLFETEGQVLLG
                     HRTPEHIYELGSFIVDGTLQSSAAAAFPELRVDPDLAEERRSIATDFAREPDKTLSFT
                     SEMPLLYGRGAAAATSYVCPMHPEVTDTKESSCPKCGMKLIPAAAAPAVTSYVCPMHP
                     EVTDTKESSCPKCGMKLIPAASVAPAASHDYDHGHGDTGDGLEWEDLMPDINRQTDLS
                     NMIWKLVDKETGAVNHEISWDFKLDERVKIRLVNEMEGSDHPMHHPFHVHGAGRFLIL
                     SRDGRPEDNLVWKDTVLLRAGEAVDILLHLTNPGLWMAHCHIAEHTESGMMFSFRVEP
                     "
     gene            complement(1990..>2420)
                     /locus_tag="VNP73_10965"
     CDS             complement(1990..>2420)
                     /locus_tag="VNP73_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HWL66484.1"
                     /translation="LGITRVTRSSRLGKRLSQRDVVIGGGLRELRKHGVTIRPRLTGA
                     EGRTATFDGGESSKIDAVLWATGYRMDHSWIDIPGLRDDEGRIKQERGVTPVAGLYTV
                     GLSWQHTRTSALLGWVTNDASFLADHIESATTRSALPGAA"
BASE COUNT          447 a          773 c          729 g          471 t
ORIGIN      
        1 atggccacgc aggacaggac gacgaagccc atggcaacga ccgactcgat cgcgataatc
       61 gagcgtcttc catcggctac cgcgaagccc acgtagacga atgcgatcgc caccagcgag
      121 actgcccaga caacgtcgtg atcaagccac cagaaggcaa ccggcgagaa cgcctgaagg
      181 atgccaacaa gcactccccc taagaggacg gcgctcgagg atcggctgcc ggcctctttg
      241 gtcagaacgc ccctcgatgt gatgccgctc atggctccac tcggaagctg aacatcatcc
      301 cgctctccgt atgctcggcg atgtggcagt gcgccatcca aagacctggg ttcgtcaggt
      361 gcaggaggat gtccaccgcc tcgccggcgc gcaggagaac ggtgtccttc caaaccagat
      421 tgtcttcagg tcgaccgtcc cgcgacagga tcaggaaacg tcccgcgccg tggacgtgga
      481 acggatggtg catgggatga tcagagccct ccatctcgtt cacgagccgg atcttgacgc
      541 gctcgtcgag cttgaagtcc caagagatct catggttcac cgcgccggtt tccttatcga
      601 ccaacttcca gatcatgttg ctgaggtccg tctgcctgtt gatgtccggc atgaggtcct
      661 cccactcgag gccgtccccg gtgtcgccgt gaccgtggtc ataatcgtgg gaagcggcgg
      721 gagcaacaga cgccgccggg atcagtttca tcccgcactt cggacagctg ctctctttcg
      781 tgtcggtaac ctcggggtgc atcggacaga cgtaagaagt gaccgcgggc gccgccgccg
      841 ccgggatcag tttcatcccg cacttcggac agctgctctc tttcgtgtcg gtaacctcgg
      901 ggtgcatcgg acagacgtaa gaagtggccg cggcggcccc cctgccgtaa aggaggggca
      961 tctcggaggt gaaggaaagc gtcttgtccg gctcgcgagc gaagtcggtt gcgatcgacc
     1021 ggcgctcctc ggcgaggtcc gggtccactc gtagctcggg gaatgcagca gcggccgagc
     1081 tctgcagcgt cccgtcgacg atgaaactcc ccagttcgta gatgtgctct ggtgtccgat
     1141 gcccgagcag gacttggccc tccgtctcga agagaacgtc gacgatggcc cgttccgatg
     1201 gcgacagcat cacttcgtcg acgaacactt ctctctcgta gcggccgctg tctcctccga
     1261 ccagcttcat gcgggctccc cggagcccga agttaaagat ccgagtgttc gccgtattaa
     1321 cgagattcag ccgcacgacc tcgccggcgg ccgctttgtc ggagaactcc gtctctccgt
     1381 tgatcagcat gacgttgccg aaacggccca tggccgtgaa ggtcgggccc gacctcctga
     1441 agggagccat ctttccgtct tcgatcaaga catcgtcaag cgtgatggtc agttcgcggt
     1501 cgacggccgg ccagtacgaa gggtcggtgg gcctgacgac gatcgtcccg gaaagaccca
     1561 tgtcctgggc atagtcctcg cggatgtgcg ggtggtacca gtagaaaccg gcgtcgggga
     1621 aatggacccg gcaggtatat gtcccaccga cctcgatcgg agcctgcgtg tccccgggca
     1681 caccgtcgta acgattctct agccgcaagc catgccagtg caccgtggcg tccacgtcgc
     1741 cctcattgtt gacatccacc gtgatcttgg agtcctggtc gacgatcaag gtggggccgg
     1801 ggatcgaccc gttgtaggcc aacattctga cctcgtcatc accgaccatc tttctcaccg
     1861 aaccgatatg aaggtcgagc cgatcgccct ccttcagacg gatgtctatc gggcgcgttg
     1921 cttgggggag acccgtggtc tcggtgctaa ataaatctga ctgcttcact gttcttcctt
     1981 ccgtgttcat cacgctgctc caggtagggc tgatcgggtg gtagctgact cgatatggtc
     2041 ggcgaggaaa ctggcgtcgt tcgttaccca tccgagtaat gcggacgtgc gagtgtgctg
     2101 ccacgacaac ccaactgtgt agaggcccgc cacgggcgtc acaccacgct cttgcttgat
     2161 gcggccctcg tcgtccctca agcccggtat gtcgatccag gagtggtcca tccgatagcc
     2221 ggtcgcccac agcaccgcgt cgatcttgga gctctcgcca ccatcgaagg tcgccgtgcg
     2281 tccctcggca ccggtcagtc gcggccggat cgtcaccccg tgcttgcgca gctcgcggag
     2341 tccgcctccg atcacgacgt cgcgctgcga tagccgcttg cccaagcgag aggaacgggt
     2401 cacgcgcgtg attccgagct
//
LOCUS       DATMED010000419         5288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4938893, whole genome shotgun sequence.
ACCESSION   DATMED010000419 DATMED010000000
VERSION     DATMED010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4938893"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VNP73_10970"
     CDS             <1..1233
                     /locus_tag="VNP73_10970"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL66485.1"
                     /translation="LGGQFVAQAADGFAQATFANVLVLEPLSAETPERILYLFALTLL
                     PYSLISPFMGVFVDRWARRSVMTAANLLRALVLITLPLWDNIAPGDGAFYVAILLLLS
                     LGRLFLTTKGALLPVILHEHHLIGGNSLSSGGGMIFALVGGAGGLFASNAIGESPLFI
                     LSGVLYIVAAYLTTRLSHPYSHPHRRSGNVGAAVAGIAVELLEGIRAIWVRIHARLAL
                     TAIFLARTIGMFVFIAAIQVIKDVYPGQYQDFGRLTTSALALGAAGLGAFVGAATAPL
                     AARRFDKGGLILLGFVVSGLGIVALGGVHNLPSLLSLTLLGGYGGFITKVAVDAQVQE
                     ALPDEFRGRAFALYDIIFNVSSVVAGALMVVTEAFSLRVTLVVTGLISLVLCAVLGML
                     MRRAGVALVSAEVSEQPA"
     gene            complement(1202..1936)
                     /locus_tag="VNP73_10975"
     CDS             complement(1202..1936)
                     /locus_tag="VNP73_10975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66486.1"
                     /translation="MTRPHPGTPFFEPIGDALGENYLRYSFTKGTEQEVTFLLELLGL
                     PEGARVLDVGCGPGRHAIGLARAGMQVTGVDISSGFLEIAQRHAADAGVRIAFYKQDA
                     RELPFEDEFDAAMSICQGGFGLMGDDDSIILRRMMEALKPGGLLVVSAFSALFEASVN
                     RPEAAFDVGAGVVHEAMTIKTAAGGEATVDAWTSVYTPRELKLLAIGVGLIPEEVWSV
                     EPGDFARREPDLERPEFMLVARKPPR"
     gene            complement(1933..3363)
                     /locus_tag="VNP73_10980"
     CDS             complement(1933..3363)
                     /locus_tag="VNP73_10980"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWL66487.1"
                     /translation="MDRSSLSGLLDPSSVAMQVARAFEAAGHSLYLVGGPVRDAWLGR
                     TAVDLDFSTDATPERSRAVVEPLASAVWLQGIEFGTVGAAIDGVHMEITTFRTERYEP
                     GSRHPDVDFTSDVEIDLSRRDFTINAMAIRLPDGSAVDPFGGAKDLAARVIRTPLDPQ
                     ASFTDDPLRILRAFRFQSQLDFNIQDDVLEAIKELKNELSTVSAERIRDELSKLVLGE
                     SVDRALESAESTSITDLFLPELGALKLEQDPVHKHKDVFHHTLAVVKRTRPVLELRLA
                     ALLHDIGKPKTRKIGPDGVSFHHHEVEGARMAEERLRALRFPNQVVLAVAEIIRLHHR
                     FHTFRLGWTDSAVRRYVRDAGPLLGLLNEMVRADCTTRNETKARRLQDRMNELEERIA
                     ELASQEELSSIRPELDGFQIMAFLGADQGPLIGQAREFLLELRLEEGELGADEVYKRV
                     EEWARERGIEPTGEKVSPRPKKETGE"
     gene            complement(3433..4518)
                     /locus_tag="VNP73_10985"
     CDS             complement(3433..4518)
                     /locus_tag="VNP73_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWL66488.1"
                     /translation="MTIDVQEVLRYLVDQKASDLHIKAGGPPYVRINGQLHKTDFPAL
                     TAADCEKAAMDIMSADQATRFKLGGEVDFAYAVGGLGRFRVNVFRQRGSVGVAVRRIL
                     PGSPSFETLGLPPIVKTLSEEHRGLLLVTGPTSSGKTTTTGAVLNHINGTRSCHILTI
                     EDPIEILHADRNAIVNQREIGLDTNTFASALRAAMRQDPDVIFVGEIRDSDTVKAALQ
                     AAETGHFVISTLHTTDVAETINRIIDFFPPHQQKQIRVSLAASLRGIVSQRLLPRKDG
                     RGRVPAVEALVMNGRIRDLILNAEQTHMIIDIVAESSFYGMQTFDQALLGLYRSGLVS
                     LDDAMAAASNQHDFQLALRQEGLQPVP"
     gene            4711..4908
                     /locus_tag="VNP73_10990"
     CDS             4711..4908
                     /locus_tag="VNP73_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66489.1"
                     /translation="METHEQTKMTRAEAGRKGGRTTKARYGEEHFGRIGKIGGKKGGE
                     TTKQRYGAEFYQRIGRLGGSK"
     gene            4998..>5288
                     /locus_tag="VNP73_10995"
     CDS             4998..>5288
                     /locus_tag="VNP73_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66490.1"
                     /translation="MRRTGVAFIAVLLVVATIESAAAQTTPADQARLRLLMQPLWHEA
                     GDRLDLKLSVDNPGTTELDGFIVTIAEHERSSSRSALHEAIDGNAGFTASSFS"
BASE COUNT          917 a         1718 c         1657 g          996 t
ORIGIN      
        1 ctggggggcc agttcgtcgc acaggcggcc gacgggttcg cgcaagccac gttcgccaac
       61 gtgctcgtgc tcgaaccgct cagcgcggaa acacccgagc gaatcttgta cctgttcgcg
      121 ctgacgctgc ttccttactc gctcatctca ccgttcatgg gcgtcttcgt ggatcgctgg
      181 gcgcgccggt cggtgatgac ggccgccaac ctgttgagag cactcgtcct catcaccctg
      241 ccgttgtggg acaacatcgc accgggagac ggggccttct atgtcgcgat cctgctcctc
      301 ctgagcctcg gccgcctctt cctgacgacc aagggcgcac tgctgccggt gatcctgcac
      361 gagcaccatc tgatcggcgg taactcgttg tcgtcgggcg gcgggatgat cttcgcgctc
      421 gtcggcggag cgggcggact gttcgcctcc aacgcgatcg gagagtcgcc tctcttcatc
      481 ctttcgggtg tgctttacat cgtcgccgca tacctgacga ctcgactcag ccacccgtac
      541 tcgcacccgc atcgccggtc ggggaacgtc ggggcagccg tcgccggcat cgcggtggaa
      601 ctcctagagg gcatccgggc gatctgggtt cggatccacg cccggctcgc gctgacggcc
      661 atcttcctcg cgcgcacgat cgggatgttc gtgttcatcg cggcgatcca ggtgatcaag
      721 gacgtctacc ccggccagta tcaggacttc ggtcgcctga ccacttccgc actcgctctc
      781 ggtgccgccg gcctcggtgc attcgtgggc gcggccacgg cccccctggc tgcccgccgc
      841 ttcgacaagg gagggctcat cctgctcggg ttcgtggtgt cgggcctcgg catcgtggcg
      901 cttggtggcg tacacaacct gccctcgctt ctgagcctga ccctcctcgg cggctacggc
      961 ggattcatca cgaaggttgc ggtggacgcg caggtacagg aagcgttacc cgacgagttc
     1021 agagggcgtg ccttcgccct ctacgacatc atcttcaacg tctccagcgt tgtggccgga
     1081 gcgttgatgg tggtcaccga agccttctca ctgcgggtca cgctcgtcgt caccggtctg
     1141 atctcgttag tcctgtgcgc ggtgctcggg atgctgatgc gcagggcggg agtcgcgctg
     1201 gtcagcgcgg aggtttccga gcaaccagca tgaactcggg gcgttcgaga tcgggctccc
     1261 ggcgagcgaa gtcgcccggc tcgaccgacc acacctcttc ggggatcagc ccaacaccga
     1321 tagccagcaa cttcagttca cgaggcgtgt agacgctcgt ccacgcatcg accgtggcct
     1381 caccaccggc cgccgtcttg atggtcatgg cttcgtggac cacgccggcg ccgacgtcga
     1441 acgcggcctc gggccggttc acggacgcct cgaagagcgc gctgaaggcc gagaccacga
     1501 gaaggccgcc gggtttcaga gcctccatca tgcgacgcaa gatgatcgag tcgtcgtccc
     1561 ccatcagccc gaatccaccc tggcagatcg acatcgcggc atcgaactcg tcctcgaacg
     1621 gcagctcgcg cgcatcttgc ttgtagaacg cgatccgaac gccggcatcg gccgcgtgac
     1681 gctgcgcgat ctcaaggaat cccgaggaga tgtcgacacc cgtcacttgc atcccggcgc
     1741 gcgcaagccc gatcgcgtgc cgtcccggtc cgcagccgac atctagcact cgtgcgccct
     1801 cgggaaggcc cagcagttcc aagaggaagg tgacttcctg ctcggtccct ttggtgaacg
     1861 agtagcggag ataattctcg ccgagggcat ctccgatcgg ctcgaagaac ggggtgccgg
     1921 ggtgtggccg ggtcactcgc cggtctcctt cttcgggcga ggtgacactt tctctcccgt
     1981 cggctcgatc ccgcgctcgc gcgcccattc ctccacgcgc ttgtagacct cgtcggcgcc
     2041 cagctcaccc tcttcgagcc gtagctcgag aaggaactca cgtgcctggc cgatgagcgg
     2101 cccctgatcg gcgcccagga acgccatgat ctggaaccca tcgagttcgg gtctgatcga
     2161 tgacagctct tcctgtgagg ccagctccgc gatgcgttcc tcgagttcgt tcatccggtc
     2221 ctgcagccgg cgggccttcg tctcattccg cgtcgtgcag tccgcacgca ccatctcgtt
     2281 aagcaggccc agcaacggcc cggcatcccg aacgtatctc cgcaccgcgg agtccgtcca
     2341 tcccaaccgg aacgtgtgga agcgatggtg cagccggatg atctcggcaa cggccaggac
     2401 gacctggttc gggaaccgca acgcacgcag acgttcctcg gccatacgag cgccttccac
     2461 ctcgtggtga tggaacgaca cgccgtccgg tccgatcttg cgagtcttcg gtttcccgat
     2521 gtcgtgcaga agcgctgcca gtcggagttc gagaaccgga cgcgtccgct tcacgaccgc
     2581 aagggtgtgg tgaaacacgt ccttgtgctt atgaacggga tcctgctcga gcttcaacgc
     2641 gccgagctcg ggcaggaaga ggtcggtgat gctcgtcgat tcagccgact cgagtgcgcg
     2701 gtcgaccgac tcaccgagaa cgagcttcga gagttcgtct ctgatccgtt ctgccgacac
     2761 tgtcgacagc tcatttttca attccttgat cgcctcgagg acgtcgtcct ggatgttgaa
     2821 atcgagctgc gactggaacc ggaacgcgcg cagtatccgc agagggtcgt cggtgaacga
     2881 ggcctgcggg tctagaggcg ttctgatgac gcgcgcggcc aggtctttgg cccccccgaa
     2941 aggatcgacg gcggagccgt ccggcaaccg gatcgccatc gcgttgatcg tgaagtcgcg
     3001 gcgcgaaaga tcgatctcga catcggatgt gaaatcgacg tccggatggc gggaaccggg
     3061 ttcgtaacgc tcggtccgga acgtagtgat ctccatgtga acgccatcga tcgcggcccc
     3121 caccgtgccg aactcgatgc cttgcaacca caccgccgaa gcgagcggct ccacgaccgc
     3181 tcgggatcgc tccggggtgg cgtccgtcga gaagtcgagg tccacggctg ttcggccgag
     3241 ccatgcgtct ctgaccgggc ctcccacgag atacagggag tggccggcgg cctcgaacgc
     3301 ccgcgcgacc tgcatcgcga ccgacgacgg gtccagcaag cctgataggg aggagcgatc
     3361 catgggtcag acggtaacgg agcgggcttc gcccgcacat tcataccccc cgctgcccca
     3421 acgcccaggt gattaaggaa ccggctgcaa gccttcttgt cgaagagcca gttggaagtc
     3481 gtgctggttg ctggccgcag ccatcgcgtc gtcgaggctt accaacccgg acctgtagag
     3541 gccgagcaac gcctggtcga aggtctgcat gccgtagaag gacgactccg caacgatgtc
     3601 gatgatcatg tgcgtctgct cggcgttcag gatcaggtcc ctgatccgtc cgttcatgac
     3661 gagcgcctcg accgcaggca cgcggccgcg cccgtcctta cgggggagga gccgttgcga
     3721 cacgatgcct ctcaacgacg cggccagact cacccggatc tgtttctgct gatgaggcgg
     3781 gaagaagtcg atgatccggt tgatggtctc ggccacgtca gtcgtgtgca aggtggagat
     3841 aacgaagtgg ccggtctcgg cggcttgtaa cgcagccttc acggtgtcgg aatccctgat
     3901 ctcccccacg aagatcacgt cggggtcttg tcgcatagca gcgcgcagcg ccgacgcgaa
     3961 agtgttcgtg tcgagtccga tctcgcgctg gttcacgatg gcattgcggt cggcatgaag
     4021 gatctcgatc ggatcctcga tcgtcaggat gtggcacgaa cgcgttccgt tgatgtggtt
     4081 gaggaccgcg ccggtcgtgg tcgtcttccc cgaagaggtc gggcccgtga cgagtagcaa
     4141 gccgcgatgc tcctcggaca gtgtcttgac gatcggcggc aacccgagcg tttcgaagga
     4201 agggctcccc gggaggatcc gtcgaaccgc gacccctacg ctgcctcgct ggcggaagac
     4261 attcacacgg aagcggccga ggccgcctac ggcgtatgcg aagtccacct cgccaccgag
     4321 cttgaagcgt gtcgcttggt cggcagacat gatgtccatg gcggctttct cgcagtccgc
     4381 agccgtcagg gcgggaaaat cggtcttgtg gagctgcccg ttgatccgta catagggcgg
     4441 cccacccgcc ttgatgtgca ggtcggaagc cttctgatct acgagatagc ggaggacttc
     4501 ttggacgtca atggtcatcg gtttctccct ggaagctaca tggaaaagtc ggcgtctcga
     4561 aaccctttct tgacctcggc cggagaatct ccacccgtcc ccaggtcgag ggtggtcccc
     4621 cgacccattc cgagcttggt ttattgcctt gctctgcggg taccactaag caaggtaacg
     4681 aacagcgagc aggcaggtca ggagggtcga atggagactc acgaacagac caagatgacc
     4741 cgagcagaag ccggcaggaa aggtggtcga accaccaagg ctcgctacgg ggaagagcac
     4801 ttcggtcgca tcggcaagat cggcgggaag aagggtggcg agaccaccaa gcagcgttac
     4861 ggcgctgagt tctaccagcg gatcggtcgc ctcggcggct cgaagtaatc ccatctttga
     4921 tctggccccc ctaacggggg gccttttcgc gtccgtatag ccgcaccgat ccccggctcg
     4981 tccttatgct cggtcagatg cggaggacgg gcgtcgcttt catcgccgtc ctgctagtcg
     5041 tcgcaacgat cgagagcgcc gcagcccaga cgaccccggc ggatcaggcg cgcttgcggc
     5101 tgttgatgca gcccctgtgg catgaagccg gagatcgtct cgacctcaag ctgtcggtcg
     5161 acaacccggg taccacagag ctagacggtt tcatcgtcac gatcgccgag cacgaacgtt
     5221 cctcctcccg ctcggccttg cacgaagcta tcgacggcaa tgccggcttc accgcgagct
     5281 cattctcg
//
LOCUS       DATMED010000420         5970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2273488, whole genome shotgun sequence.
ACCESSION   DATMED010000420 DATMED010000000
VERSION     DATMED010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273488"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..135
                     /locus_tag="VNP73_11000"
     CDS             25..135
                     /locus_tag="VNP73_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWL66491.1"
                     /translation="MDPRIVEIFAKWGFAWGGHWSTPDPMHFELSRLLPS"
     gene            complement(132..1085)
                     /locus_tag="VNP73_11005"
     CDS             complement(132..1085)
                     /locus_tag="VNP73_11005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWL66492.1"
                     /translation="MTRLGFTGPVFGFDVRATSELARKAEGMGYTDCWTAETSGPDGF
                     SVASAVGVSTKSLRLGIAIAPVYTRPPALMAMSALAVYQATEGRFCLGLGASSPVIVE
                     GWMGEKLERPLTYMREAVEVVRSAFAGDKVDFKGTTATVRGFRLDGAPKEQLPIFLAA
                     LGPQMMALTNEIADGIVLYLATEEGVRIAAKACPGKEIAERIMVCPDEDVDQVRGAVR
                     WMLTPYVAVPAYNRFIAAQGFEDEARQVADAWKNNEREKARDAVSDRLIDALVIMGSA
                     DECKERLESFREAGLVTPILAPFSLTGDPKKVEAALKSLAP"
     gene            1118..1846
                     /gene="ccsA"
                     /locus_tag="VNP73_11010"
     CDS             1118..1846
                     /gene="ccsA"
                     /locus_tag="VNP73_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWL66493.1"
                     /translation="MRSPSSAPPRGDALRRLGIVTAVAMTAALILIFFVAREADAAMG
                     GPLQRIFYFHVPAAWAAYLAFAIVFLSSIAYLRMGGRRWDLLAESAAELGVAFTTIVL
                     VTGPIWAYPVWGTWWQWDARLTSALVMWLTYVGYLFLRALTEDPQRVGRLAAVAGVVG
                     FVNVPIVHFSVRWWRTLHPSGPTPADLAADSGLGSPELLAFFTSLIAFTLLFAWLLGV
                     RVRLGRLSDEIGAKEMRAPIEQAA"
     gene            1843..1977
                     /locus_tag="VNP73_11015"
     CDS             1843..1977
                     /locus_tag="VNP73_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HWL66494.1"
                     /translation="MSNTAWLFVAFLAAWAIIGAYVYSLGARQRRLEERLNSLERSRS
                     "
     gene            2055..2426
                     /locus_tag="VNP73_11020"
     CDS             2055..2426
                     /locus_tag="VNP73_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66495.1"
                     /translation="MSIPERVYQYTDRPMTWTKAVLLGFVVWVAAIVLLGQVPSVIIY
                     KFDQYIAEIIDFTKKIPGVNEAGLNTIQIKMIRDVIANSVQMGILTGMFVGAYIWQKR
                     KQKRTGAKGLADPVKGYMPGK"
     gene            2434..2856
                     /locus_tag="VNP73_11025"
     CDS             2434..2856
                     /locus_tag="VNP73_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWL66496.1"
                     /translation="MPKPPDPKKMEIIEKPIFRDDYAMEMVDADWLKGAVKPKNFIDF
                     IPEQCILCEGCVDICPWYCIFMVSTETVADAGNVELARHMEHDDILFLIDDNACTRCN
                     LCVERCPTGALVQSTTSGAKKSRGARAMGAVPSSQKAS"
     gene            2856..3626
                     /gene="extP"
                     /locus_tag="VNP73_11030"
     CDS             2856..3626
                     /gene="extP"
                     /locus_tag="VNP73_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HWL66497.1"
                     /translation="MATAGWNPRDKMNERLDQLQNSQVWRSIFRPGSIFRRGYADSPR
                     NRAYVVMNSLLYHLHPVKVKRHGIKLSYTLCLGGLSFFLFILLTITGIYLMFFYRPEV
                     SVVYADMQRLSAQVSFGLLVRNLHRWGAHLMVISVFLHMARVFYTGAYKPPREFNWIV
                     GVILFLLTLLLSFTGYLLPWDQLAVWAVTVGTEMMKNSPVIGDQVQFALLGGPVIGPE
                     TLIRWYTLHVWLLPFVIVIFISIHFWRVRKDGGISGPM"
     gene            3639..4445
                     /locus_tag="VNP73_11035"
     CDS             3639..4445
                     /locus_tag="VNP73_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HWL66498.1"
                     /translation="MPTPDEIYEQVLAEEQGKGSSPAVAQARAKAARVRAQKGAADPT
                     KAQASVPAGGGAPSTSETPQAQSAAGTPPSSAAQPAAAAKPTPKAPAKKAVGNGGPAV
                     PERVQRLLAVVKPEAIQKIERQPMDRVNVWPHLMAAEFLALLVIMALLTMFSVAVDAP
                     LRDLANFSQTPNPSKAPWYFLGLQELLRYFHPQVAGVTIPTVLILAWFVWPFIDRTPS
                     TRPEDRKVAIVLFTFYVITFAILSTIGMFFRGPGFNFIFPWETGPIFFEL"
     gene            4510..5313
                     /locus_tag="VNP73_11040"
     CDS             4510..5313
                     /locus_tag="VNP73_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWL66499.1"
                     /translation="MSPGTIALIIVAVSIAAWAAFMANVTIRKQRKALAGAGDTRAHP
                     VEGEGAAEPAKAQAAADTAPSKPQKAPPPPPLTPDQLAVTRRQFLNRAWASSFSAFLG
                     FFGMASLSFLWPTLTGGFGTKITVGDYEEILKEVGPENDFKPKFYSEGRFWLTYYEGT
                     GEAPVYLAVNAGETKLLALYRKCVHLGCSVPHCGTSMLFECPCHGSKYRLTGEYYGGP
                     APRGLDRFPVTIEGGKVVVDTGDVQEGPPRGTSTWEKFSEPVGAFCVPT"
     gene            5330..>5970
                     /locus_tag="VNP73_11045"
     CDS             5330..>5970
                     /locus_tag="VNP73_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66500.1"
                     /translation="MSQGDVILITLIVLGPATILWALYLMRTKRSRKPAAALGIPHAM
                     RPAAPDEVLEGPRLERIQWGGVASLVLLLIFLGAYWLPEPQRHEAFAHRFEEEALHRG
                     ELTYNVAPQLEEDISAQKFKEEEKALSLGQGCANCHGPEGVGGIGNPLFKNPATGDTV
                     LYQAPPLNNVFSRWDEEVVRFTIERGRPGTPMPAWGVEYGGPMTEQMVDDVMT"
BASE COUNT         1099 a         1935 c         1816 g         1120 t
ORIGIN      
        1 aaccactacg gcacgcgcgg ggacatggat ccgcggatag tcgagatctt cgctaagtgg
       61 ggtttcgcct gggggggcca ctggtccacc cccgacccca tgcacttcga gttaagccgt
      121 ttgctaccga gctaaggagc cagagatttc agagcggctt cgactttctt gggatcgccc
      181 gtcagcgaga acggcgccag gatcggcgtt accaggccgg cctcacggaa cgactccaat
      241 ctttccttac actcgtcggc cgaacccatg atcaccagcg cgtcgatcaa acgatccgat
      301 accgcgtcgc gcgccttctc acgctcgttg ttcttccagg cgtcggcaac ctggcgcgct
      361 tcgtcctcga agccttgcgc cgcgataaac ctgttgtaag cgggcaccgc gacatacggc
      421 gtcaacatcc accgcaccgc tccgcgaacc tgatcgacat cctcgtcggg gcacaccatg
      481 atccgctcgg cgatctcttt gcccgggcac gccttcgccg cgatgcgcac cccttcttcg
      541 gtcgctagat acagaacgat cccgtcggca atctcgttag tgagcgccat catctgtgga
      601 ccgagtgccg cgaggaagat cggcaactgc tctttcggcg cgccgtccaa acggaaacct
      661 ctcaccgtcg ccgtcgttcc tttgaagtcg accttgtcgc cggcgaacgc gctgcgaacg
      721 acttcgaccg cctcacgcat gtacgtcaac ggacgctcga gcttctcgcc catccaacct
      781 tcgacgatca cgggcgagga agcgccgagc ccgagacaga atctgccttc cgttgcctga
      841 taaacggcga gggcgctcat cgccatcaat gcaggaggac gcgtgtagac gggagcgatc
      901 gcgatcccca ggcgaagcga cttcgtcgag acaccaaccg ccgacgcgac cgagaaccca
      961 tcgggcccgg acgtctctgc ggtccagcag tcggtgtagc ccatgccttc ggccttgcgc
     1021 gccagttcgc tggtcgcgcg cacatcgaat ccgaatacgg gaccggtgaa cccgaggcgc
     1081 gtcataggga gaggacatta gtagtgtcga acgcatcatg cgaagtccta gttcggcccc
     1141 cccacgggga gacgccctaa gaaggctggg catcgtgacc gcggtggcca tgacggccgc
     1201 cctgatcctc atcttcttcg tggctcgaga ggccgatgca gctatgggag ggcctctaca
     1261 gcggatcttc tatttccacg tcccggccgc ctgggccgcc tacctcgcct tcgcgatcgt
     1321 gttcttgtcc tcgatcgcat acctgaggat gggaggtcgg aggtgggacc tcctggccga
     1381 atcggcggcc gagctgggtg tcgccttcac cacgatcgtg ctcgtcacgg gcccgatctg
     1441 ggcctatccc gtgtggggaa cctggtggca gtgggacgct cggctgacct cggccctcgt
     1501 catgtggctg acctacgtcg gatatctgtt ccttcgcgcc ctgaccgagg acccgcagcg
     1561 cgtaggtcgc cttgctgcag ttgccggggt cgtcggtttc gtgaacgtac cgatagtcca
     1621 cttctcggtt cgctggtggc gaacgctcca tccatcggga cccactccgg ccgacctcgc
     1681 cgcggactcc ggcctgggaa gtccggagtt gcttgccttc tttacctcgt tgatcgcgtt
     1741 caccctgctc ttcgcctggc tgctaggagt gcgcgtccgt ctcggtcgac tctccgacga
     1801 gatcggcgcg aaggagatgc gcgccccgat cgaacaagcg gcatgagcaa cacggcctgg
     1861 cttttcgtcg cattcctcgc cgcctgggcg atcatcgggg cctacgttta ctcgctcggc
     1921 gcgcgccagc gacggttgga ggaaaggctc aattcactgg agcgttcgcg ctcctaagtg
     1981 cttgtgcata agccctgctc aaatgcacaa tctcgccggt gggagcgggg gtctcctatg
     2041 taagatggcg tcgcatgagc atcccggagc gggtctacca gtacaccgac cgccccatga
     2101 cctggaccaa ggcggtgttg ctgggattcg tggtctgggt ggccgcgatc gtgctgctcg
     2161 gacaggttcc ctcggtcatc atctacaaat tcgaccagta catcgccgag atcatcgatt
     2221 tcacgaagaa gatccccggc gtgaacgaag caggcctaaa cacgatccag atcaagatga
     2281 tccgcgatgt gattgccaac agcgtgcaga tggggatctt gacgggcatg ttcgtcggcg
     2341 cctacatctg gcagaagcgc aagcagaagc gcaccggcgc caaaggcctc gcggatcccg
     2401 tcaagggcta catgccgggg aaatagggag accatgccga agccaccgga ccccaagaag
     2461 atggagatca tcgagaagcc catctttcgg gacgactatg cgatggagat ggtcgacgcc
     2521 gactggctga agggtgcggt caagccgaag aacttcatcg acttcatccc ggagcaatgc
     2581 atcctgtgtg agggttgcgt cgacatctgc ccctggtact gcatcttcat ggtttccacc
     2641 gagaccgtcg ccgacgccgg aaatgtcgag ctcgctcgac acatggagca cgacgacatc
     2701 ctcttcctca tcgatgacaa cgcctgcacg cgctgcaacc tgtgcgtcga acgctgcccc
     2761 accggcgcat tggttcagag caccacctca ggagctaaga agtcccgagg tgcccgcgct
     2821 atgggcgccg ttccctcgag ccagaaggct tcgtaatggc gaccgcaggc tggaaccctc
     2881 gagacaagat gaacgagcgg ctggatcagc tgcagaactc gcaggtctgg cgctcgatct
     2941 tcagaccggg ctcgatcttc aggcgcggat atgcggactc cccgaggaac cgcgcctacg
     3001 tcgtcatgaa ctcgttgctg taccacctgc atccggtcaa ggtgaagcgc cacgggatca
     3061 agctgagcta cacgctgtgc ctcggagggt tgtcgttctt cctgttcatc ctgctgacca
     3121 tcaccggcat ctacctgatg ttcttctatc ggccggaagt aagcgtcgtg tacgccgaca
     3181 tgcagagatt gtcggcacag gtctcgttcg gtcttctggt tcggaacctg caccggtggg
     3241 gtgcccacct gatggtgatc agcgtgttcc tccacatggc tcgtgtcttc tacaccgggg
     3301 cttacaagcc gccgcgtgag ttcaactgga tcgtcggcgt gatcctgttc ctgctcacgc
     3361 tgctcttgtc gttcaccgga tacctgttgc cgtgggacca gctcgcggtg tgggccgtca
     3421 cggttggcac cgagatgatg aagaactccc ccgttatcgg ggaccaggtg caattcgcac
     3481 tgctcggagg cccagttatc ggccccgaga cgctgatccg ctggtacacc ttgcacgtgt
     3541 ggctgttgcc gttcgtgatc gtgatcttca tctctatcca tttctggaga gtcaggaagg
     3601 acggtgggat ctcgggcccg atgtagcccg gagagacgat gccgacaccc gacgagatct
     3661 acgagcaggt actggccgag gagcagggca aaggctcctc accggccgtc gcgcaagcgc
     3721 gagccaaggc cgctcgcgta cgtgcccaga agggtgccgc cgatccaacc aaagcccagg
     3781 ccagtgtgcc tgcggggggc ggcgccccgt cgacctccga aacccctcaa gcccagtcgg
     3841 ccgccggcac gccgccctcc tcggccgcgc aacctgctgc tgcggccaag cccacaccca
     3901 aagctcccgc taaaaaggcg gtcgggaatg gcgggcccgc cgtgccagaa cgggttcaga
     3961 ggttgcttgc agtcgtaaaa ccggaggcca ttcagaagat cgagcggcaa ccgatggacc
     4021 gcgtcaacgt gtggccgcac ctgatggccg cggagttcct agcgttgctc gtcatcatgg
     4081 cgttgctgac gatgttctca gtcgcggtcg atgctcccct gcgtgacctc gccaacttca
     4141 gccagacccc gaatccgtcg aaggcgccgt ggtacttcct cggcctgcag gaactgcttc
     4201 gttatttcca cccacaggtc gcaggagtga cgatcccgac cgttctcatc ctggcgtggt
     4261 tcgtatggcc gttcatcgac cgcaccccct cgacgagacc cgaagaccgc aaggtggcga
     4321 tcgtgctgtt cacgttctac gtgatcacgt tcgcgatcct gtcgacgatc gggatgttct
     4381 tcagaggccc cgggttcaac ttcatcttcc cgtgggaaac cggccctatc ttcttcgagc
     4441 tatgacgaga accgacgcaa gaaaacgcgc ccgaacgccg atggtctcga ccatcggcgg
     4501 gggaaaataa tgtcaccagg aactatcgcc ctcatcatcg tcgcagtgag catcgccgcg
     4561 tgggcggcgt tcatggcgaa cgtgacgatc cgcaagcagc gcaaagcact ggcgggcgcg
     4621 ggagacacgc gtgcccatcc ggtcgaaggc gagggcgcag ccgaaccggc caaggcacag
     4681 gccgcggccg acaccgcgcc ctcgaagccg cagaaggctc caccgcctcc cccgctgacg
     4741 cccgatcaac tcgccgtcac gcgccgtcag ttcctgaacc gcgcgtgggc gtcttcgttc
     4801 tcggcgttcc tggggttctt cgggatggct tccctctcgt tcctgtggcc gacactcacc
     4861 ggaggattcg gaacgaagat caccgtcggc gactacgaag agatcctgaa agaggtcggc
     4921 cccgagaacg acttcaagcc caagttctat tccgagggcc ggttctggct gacgtattac
     4981 gaaggcacgg gcgaggcccc ggtttatctc gccgtcaacg cgggagaaac caaactcctc
     5041 gccctttacc ggaagtgcgt ccatctcggt tgttcggttc cgcactgcgg cacgtcgatg
     5101 ttgttcgagt gcccgtgcca cggttccaag taccggctga cgggtgagta ctacggcggc
     5161 ccggcccccc gcggactgga tcgcttcccc gtcacgatcg aaggcggcaa ggtcgtcgtc
     5221 gacaccggtg acgttcagga agggccgcct cgcggtacga gcacctggga gaagttctcc
     5281 gagcccgtcg gcgcgttctg cgtcccgacg tgatggggat cgcgactaga tgagtcaagg
     5341 cgacgtaatc ctcataactc ttatcgttct tggaccggcc acgatcctgt gggcgctcta
     5401 tctgatgcgc accaagcgga gccgcaagcc ggccgccgcg ctcggcatcc cgcatgcgat
     5461 gcgacccgct gcaccggacg aggtcctcga agggccccgg ctcgagcgga tccagtgggg
     5521 cggcgttgca tccctcgttc tcctgttgat cttcctgggc gcctactggc tgccggagcc
     5581 acagagacac gaggcgttcg cgcaccgatt cgaggaagag gcgctgcacc ggggcgaact
     5641 cacgtacaac gtcgcaccgc aactcgaaga ggacatctcc gcacagaagt tcaaggagga
     5701 agagaaggcg ctttcactcg gccaaggctg cgcgaactgc cacgggcccg aaggggtggg
     5761 gggaatcggt aacccgttgt tcaagaaccc ggctacgggc gacaccgttc tctaccaggc
     5821 gccgcccctg aacaacgtct tctcgcgctg ggacgaggag gtcgtgcgct tcacgatcga
     5881 gcgcggccgc ccgggaactc cgatgccggc gtggggggtg gagtacggcg gcccgatgac
     5941 cgagcagatg gtcgacgacg tcatgacctg
//
LOCUS       DATMED010000421         4076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4177846, whole genome shotgun sequence.
ACCESSION   DATMED010000421 DATMED010000000
VERSION     DATMED010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4177846"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VNP73_11050"
     CDS             complement(<1..290)
                     /locus_tag="VNP73_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWL66501.1"
                     /translation="MNIVFTPSQKPTLGVEIEVQIVDESGALATDTAATKILAELDDD
                     VGFKHELLECCVEVITGVCDTVAEVEADLGSKIKRLAEVADGFGYRIICTGT"
     gene            406..2556
                     /locus_tag="VNP73_11055"
     CDS             406..2556
                     /locus_tag="VNP73_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWL66502.1"
                     /translation="MTILTGPSRALILLVAMALIAVSQAANAADRPSRARPAGAIALP
                     AYVEHEVIVAYRSGIPTTWRSLARRSVGGGLRVRIPRSGAEVVRLAPHLDVADAVELL
                     ENRPEVLYAEPNYIYRAGDVPNDPLADATWPLIDATSDVDIDVAAAWSLSTGDSDVVV
                     AVVDTGIGLEHPDIEGNLWSNPGESLNGVDDDGNGLVDDVHGWDWIDDDAIPADLHGH
                     GTHVAGTIGARGNNSIGISGINWEVGLMPLRMLDARGLGTTADFASAIAYADENGADV
                     LNASLGGPKDSRVVADAIRASGMVFVVAAGNSGADLDSTPDYPCSLGYAKLLCVAASD
                     GDDDLADFSNYGTDDVDLAAPGTNIFSLAPSLETLFAENFEGTPTDRWISGGVKDGWG
                     FDSDQFGAYASDSPDGDYLNDTDAWLQTREGVDLSGTTGCRLRFMRDIDTEKGADKLL
                     VETSTGHGWDAVSSISGESSGWRPANVELGSGAPNDLLLRFRFVSDDSRPDGGVEIDN
                     VKIDCATSDYSNSSYATLSGTSMASPHVAGAAALILAIVPDASAVEIAGAINEGVDKV
                     SGLEGEVSSGGRLSVVGALIALGRDIPVPDETDLPDVPDPIGTATPSPIASPTVAPVV
                     RSDRTVSLVLKRKLVATGRVLSAGPDGCVSSVAIKLFRNGRFLRETRTGRGGWFRLEL
                     RDRPGSYRAVAPRLELSPRDICAVARSIQLRRFL"
     gene            2748..>4076
                     /locus_tag="VNP73_11060"
     CDS             2748..>4076
                     /locus_tag="VNP73_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWL66503.1"
                     /translation="MRLAGKISILVAVTCVAALLAPGASAAPKEYEIQVQTYTIETKH
                     GLIYTEIAHPMRGTDIAKGPVILTLSPYSALGREANRTRWVPKGYHVAFADVIGTGNS
                     GGCYDYGGNREKETAHDIVEWMGTQKWSTGKVGMIGGSYNGTTQHAAAVTHPKHLTTI
                     VPEAAIARWYDYAYRGGIRYFLNNEEPADEGFDTPFAFDFGFAIPPPVDVEDPQWADK
                     VQSTMTPCEELEHTQHGYDDTPDYDEFWVERDYERLAHTVKIPVLVAHNWGDWNVKQA
                     NGWDMFKAYPNAWMYFGDRYMTHGTPDGDYDATVDAWFDHWLMGKDNGIEKMPRITSE
                     TANWDGPLKYLSADKVKTTPVKLYMQATPRTGPEDYEWKLLPSEPFAGLQPAVSEFPS
                     AGINTEGHANHHARSHHDWWWWESPALKKDTRIFGEIKVKLNLTSDREWIT"
BASE COUNT          803 a         1331 c         1266 g          676 t
ORIGIN      
        1 tgggtgccgg tacagatgat ccggtagccg aacccgtccg ccacctcggc cagcctcttg
       61 atcttgctcc cgaggtcggc ctccacctct gcgaccgtgt cgcagacccc ggtgatgacc
      121 tcaacgcagc attcgaggag ctcgtgcttg aaccccacat cgtcgtcgag ctcggccagg
      181 atcttcgttg ccgcggtgtc ggtggccagc gcccccgatt cgtcgacgat ctggacctcg
      241 atctcgaccc cgagcgtcgg cttctgcgag ggagtgaaaa cgatattcat gagttgtcgc
      301 ctttcctaac ccgagaccac ccggcgttct atcagcgccg cccaccgtct aagtagtact
      361 gcgtataccc atcaaggcct ctttgtccta ctttgtccac agaccatgac gatactcacc
      421 gggccttcgc gcgcgctcat cctgctggtc gcgatggccc tgatcgccgt gtctcaggcg
      481 gcgaacgcgg ccgaccggcc ttcacgcgcc cggccggccg gcgcgatcgc actccccgcg
      541 tatgtcgaac atgaagtcat cgtcgcctat cgatcgggga tcccaaccac gtggcgcagc
      601 ctcgctcgcc gcagtgtggg agggggtctg agggtccgta tcccccgatc cggagccgaa
      661 gtggtccgac tagctcctca tctggatgtt gcagacgcgg tggagctcct ggagaacaga
      721 cccgaggtcc tgtacgcgga acccaactac atctatcgcg ccggcgacgt gcccaacgac
      781 ccgctcgccg acgctacgtg gccgctgatc gacgcaactt cggatgtcga catcgacgtc
      841 gcagccgcgt ggagcctttc taccggcgac tccgacgtcg tagttgcggt tgtggatacc
      901 gggatcgggc tcgaacaccc cgacatcgag ggcaacctct ggagcaatcc cggggaatcg
      961 ttgaacggcg tcgatgacga cgggaacggt ctcgtcgacg acgttcatgg gtgggattgg
     1021 atcgacgatg acgcgatccc tgcagacctg cacggacatg ggacgcatgt cgccgggacc
     1081 atcggagcac gggggaacaa ctcgatcggc atctcgggga tcaactggga ggtcgggctc
     1141 atgcctttgc ggatgctgga cgcgcgcggg ctcggtacca cggcagactt tgcgagcgcg
     1201 atcgcgtacg cggacgagaa cggcgccgac gtccttaacg caagcctcgg aggtcccaag
     1261 gattcgcgcg tcgttgcgga cgcgatccga gcatccggga tggtcttcgt tgtcgccgcc
     1321 ggcaattcgg gtgcagatct ggattccacc cccgattacc cttgcagcct cggatacgcc
     1381 aaactcctgt gcgtcgccgc gtcggacgga gacgacgacc tggcggattt ctcgaactac
     1441 ggcaccgacg acgtcgatct cgcggccccc ggaacgaaca tcttcagcct ggcgcccagc
     1501 ctcgagacgt tgttcgcgga gaacttcgag ggcacaccta ccgatcgctg gatcagcggt
     1561 ggagttaagg acggctgggg cttcgactcc gaccagttcg gtgcctatgc aagcgacagc
     1621 cccgatggcg attacctcaa cgacacggat gcctggctcc agacccggga gggggtcgat
     1681 ctgtccggta ccaccggctg ccgccttcgc ttcatgaggg acatcgacac ggagaaagga
     1741 gccgacaaac tcctcgtcga gacatctacg ggccacggat gggatgcggt ctcatcgatc
     1801 tccggcgagt cctccggatg gagaccggcg aacgtcgagc tcgggagcgg cgcgccgaac
     1861 gatctgttgc tgcggttccg tttcgtatcc gacgacagcc gacccgatgg aggcgtcgag
     1921 atcgacaacg tgaagatcga ttgcgcaaca tccgattact cgaactccag ttacgcaacc
     1981 ctgagcggaa cctcgatggc gtcaccacac gtcgcggggg ccgctgctct gatcctggcg
     2041 atcgttccgg acgcgagtgc cgttgagatc gcaggggcga tcaatgaagg agtcgacaag
     2101 gtgtccggcc tggaggggga ggtttcttcg ggaggtcgct tgagcgttgt cggcgctctg
     2161 atcgccctcg gtcgggacat cccggtgccg gacgagaccg accttcccga cgtaccagat
     2221 ccgatcggca ccgcgacccc gtcacccatc gcctccccga ccgtggcccc cgtggtgcga
     2281 tccgatcgaa ccgtctcgct agtcctcaag cgaaaactgg tcgcgacggg gagagtgctg
     2341 tcggcggggc cggacggatg tgtctcgagc gtagcgatca agctgttccg caacggccgc
     2401 ttcctgcgcg agacgcgcac cggaaggggg ggctggttcc gactcgagct cagggatcgg
     2461 ccgggtagct accgtgccgt cgccccccgt ctcgagctga gcccgcggga catctgcgct
     2521 gtggctcgat cgatccaact caggcgtttc ctctagggaa ggagccgcga tccgaccgaa
     2581 aaaggacaaa agcccaggtc acacggctta aaacggggtc acggcaagca ggaccgcgcc
     2641 ccctatcgga gaaacttgta gcagggagcc gggatccgga tcccacaccc ggttcgaacc
     2701 gaaggggacg aatcggacag gacgaacgag ggggtaagga actgaagatg cgcctagccg
     2761 gaaagatctc gatcctggta gccgtcacgt gcgtggcagc tcttctcgcg ccgggtgctt
     2821 cggccgctcc caaggaatac gagatccagg tccagaccta cacgatcgaa accaaacacg
     2881 gactgatcta caccgagatc gcacacccga tgaggggcac cgacatcgcc aagggtcccg
     2941 tcatcctgac tctgtccccc tattcggcac tcggtcgcga ggcgaaccgc acccgctggg
     3001 tgcccaaggg ttatcacgtc gccttcgccg acgtgatcgg gaccggcaat tcgggcggct
     3061 gctacgacta cggcggcaac cgcgagaaag aaaccgcaca cgacatcgtc gagtggatgg
     3121 ggacccagaa gtggtcgacc ggcaaggtcg gcatgatcgg tgggtcctac aacggcacga
     3181 cccagcacgc ggccgcagtg acccacccca aacacctgac gacgatcgtt cccgaagctg
     3241 cgatcgcccg ctggtacgac tacgcgtacc gcggcgggat ccgttacttc ctcaacaacg
     3301 aagaacctgc cgacgaaggt ttcgacacgc cgttcgcgtt cgacttcggc ttcgcgatcc
     3361 caccgcccgt cgacgtcgag gacccacaat gggccgacaa ggttcagtcg acgatgactc
     3421 cgtgtgagga gctcgagcac acccagcacg ggtacgacga cactccggac tacgacgagt
     3481 tctgggtcga gcgggactac gagcgccttg cacacacggt caagatcccc gtactggtcg
     3541 ctcacaactg gggcgactgg aacgtcaagc aggcgaacgg ctgggacatg ttcaaggcat
     3601 acccgaacgc gtggatgtac ttcggcgacc gctacatgac tcacggcacg cccgacggcg
     3661 actacgacgc gaccgtcgat gcctggttcg accactggct gatgggcaag gacaacggca
     3721 tcgagaagat gccccgcatc acgtccgaga ccgccaactg ggacggaccg ctcaagtacc
     3781 tgtcggccga caaagtgaag acgacgccgg tcaagctcta catgcaagca acgccccgca
     3841 ccgggcccga ggactacgaa tggaagctgc tacccagcga gcccttcgcc gggctccagc
     3901 cggccgtctc cgagttcccg tcggccggga tcaacaccga gggccatgcc aaccaccacg
     3961 cgcgtagcca ccacgactgg tggtggtggg agtcaccggc tctcaagaag gacacacgga
     4021 tcttcggcga gatcaaggtg aagctgaacc tcaccagcga tcgcgagtgg atcaca
//
LOCUS       DATMED010000422         4861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5713232, whole genome shotgun sequence.
ACCESSION   DATMED010000422 DATMED010000000
VERSION     DATMED010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5713232"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..620
                     /locus_tag="VNP73_11065"
     CDS             192..620
                     /locus_tag="VNP73_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66504.1"
                     /translation="MIRKTFALLITAGLVALPALAEAGKARTATGNYNTIMITPEEGS
                     PSAEGHYSNGVKFKLRPGERFVSLSIADEAGQTVRATVGQDYDNDGKTEHEHEFCGST
                     TEPIKLTPGAPVIVWAQEGQCADGTTSLATFGEVTATFTR"
     gene            838..1890
                     /locus_tag="VNP73_11070"
     CDS             838..1890
                     /locus_tag="VNP73_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWL66505.1"
                     /translation="MPNAPAGSYNPEGAFWTGERFLVVAGATVETWNPNRNEWKVVAQ
                     IPQADDCEGCGYGETAVWTGEELLLWGGGFSFRAPNGSKHKGVSVDLEGEVVPLPDAP
                     IPVRRWHQAVWTGSEMITFGGGSDSVARNDGAAYNPGTRTWREIARSPVGGYVNTLVW
                     TGKEMITWGGIEDATTSTHGYPSGFIAQGAAYDPAADEWRRLAGSGLDPRGGHSAVWT
                     GEEMIVWGGLSRIPTTCHDCGYPTDAGAYNPSTDKWRAIRLGPMSGRVDHTSVWTGQE
                     MIVYGGSTPGGSRRRNSAGLYDLAADRWRKLPEPPIAGRNFHVAVWTGTEMLVWGGQK
                     PNGQPFSDGVLFVPDW"
     gene            complement(1856..4030)
                     /locus_tag="VNP73_11075"
     CDS             complement(1856..4030)
                     /locus_tag="VNP73_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HWL66506.1"
                     /translation="MPRRIPYVQQLEISDCGAACLAMNLAFHGRNVSPEDVRRTTRTS
                     RDGVSARSLVEAARAYGMDARGVSVELEGLKYLERGSILHWEFNHFVVLDRVTRKGVE
                     VLDPALGRVRLSLEKVSKSFTGVAIVLKPTTEFERGGASGGAWRYLKPMLTQSRLLSK
                     VGVTSLLIQLFALATPILMGVVVDRVVPPPGDRDLLLAVGLGMLAMVGFYLLSTYVRS
                     HLLLALRAQLDMNISTGFIGHLVKLPYQFFLQRSAGDLMARLNSNITVREILTTGAIS
                     SVLDGALVSIYLLLIFAQSVVMGAVVVALAVLQVAILAFTRRPMQRVMAETLQAQARS
                     AGYLGQLVTGIETLKSVGAESRGVAQWSNLFVDEINATLARGRLSATIETLTTSLRVA
                     SPLIVLTVGALGVLSGDLTLGTMLALSAMATGFLTPLSSLIITGTQLQLLGSYMERIN
                     DVLDTAPEQNPAEVEPAGRLGGRITLNEVGFRYAETAPEVVQGVSLEIEPGKMVGVVG
                     RSGSGKSTLAHLILSLYKPTRGAVLYDGRDLAELEATTVRAQLGIVPQNSYLFGTSVQ
                     TNISLTKPEATFEEIERAARLACIHDDIMAMPMGYDTVLSDGGASISGGQRQRIALAR
                     ALVHAPAILLLDEATSSLDAVTEKAIYQNLQRLDCTRIVIAHRISTIARADLIVVMED
                     GRFVEQGTHDELVAAGDLYSELAASQTASDGITNQERKAPHR"
     gene            complement(4023..4523)
                     /locus_tag="VNP73_11080"
     CDS             complement(4023..4523)
                     /locus_tag="VNP73_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66507.1"
                     /translation="MTDGSSIFRPEALEARARVQTPGDVVRLGATWTTWAFWGLVALV
                     AVALIAATQIEIRSFATGPTSRLGRRVAVLVPGPAAAEVVKGSSVLIGGREARVIAAG
                     GRPMSTALVERRFGVEVDEPSVLIVTSARATASATGIARVEVGSDPVIVALVPGLRAL
                     FGDDDA"
     gene            4619..4789
                     /locus_tag="VNP73_11085"
     CDS             4619..4789
                     /locus_tag="VNP73_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66508.1"
                     /translation="MDTTLTSDGPKLRELFIEELAEVRAGSSQGGGNTTMACCEEGPF
                     GCCDILPIDPID"
BASE COUNT          930 a         1548 c         1565 g          818 t
ORIGIN      
        1 ggccgagttc ctcgccgagc cggtcgataa cggctacgag atccgagcct tcttccggga
       61 cccggacggg catctcttcg agatcagcca ggtcgcggga taggcccggc gcactagggc
      121 gtgacctttc tgggcccgct gagtactaga taagagacgg cgggacaacc acgtgatcca
      181 ggaggacgaa aatgatccgg aagaccttcg cactgttgat aacagcgggc ctcgtggccc
      241 taccggccct ggccgaggcg ggcaaagctc gtacggcgac cggcaactac aacaccatca
      301 tgatcacccc cgaagagggc tccccttcag ccgagggcca ctactcgaac ggcgtgaagt
      361 tcaaacttcg gccgggcgag cgcttcgtat ccctatcgat cgccgacgag gccggacaga
      421 cggtgcgagc cacggtcgga caggactacg acaacgacgg caagaccgag cacgagcacg
      481 agttctgcgg ctcgaccact gaaccgatca agctgacgcc gggcgctccg gtgatcgtgt
      541 gggcgcaaga gggccagtgc gccgacggga ccacctcgct ggcaacgttc ggagaagtga
      601 cggcaacctt cacgaggtag cggaacctcc ctctctggac atcgctcagt tacgtccgaa
      661 ccatcgcgcc cgtccctgcg tcctatctgc atgattgctg ctgcccgtaa gaggtggttg
      721 gtcaccgccg tcttgttcct cgctgcttgc acaaaggacg tttcggctcc tctggcagag
      781 ccacagccct ctgcccaccc gtccaggaca ttgaggtcgg gcttttgggc catcactatg
      841 ccaaacgcac ccgcgggttc gtacaacccg gagggcgctt tctggaccgg cgaacgcttc
      901 ctggtcgtcg ctggagccac tgtcgagacg tggaacccaa accgcaacga atggaaggtc
      961 gttgcccaga tccctcaggc cgacgactgc gagggctgcg gctacggcga gaccgccgtg
     1021 tggaccggag aggagctgct tctctgggga ggaggctttt cctttcgagc gcccaacggc
     1081 agcaagcaca aaggcgtgtc ggtagatctc gaaggcgaag tagtgcccct tcccgatgcg
     1141 ccgatcccgg tccgtcggtg gcaccaagct gtttggaccg gatccgagat gatcaccttc
     1201 ggcgggggat ccgattctgt cgcccgtaac gacggagccg cctacaaccc tggcactcgg
     1261 acttggcgag agatcgctcg ctccccggta ggcggctatg tcaacacctt ggtgtggact
     1321 ggcaaggaga tgatcacatg gggcgggatc gaagatgcga cgacaagcac gcacggatat
     1381 ccctccgggt tcatcgctca aggagcggcc tatgacccgg ccgccgacga atggcgccga
     1441 ctggccggtt cgggtctcga tccacgtggg gggcacagcg cggtttggac gggcgaggag
     1501 atgatcgtgt ggggcggact ctcgcggatc ccgacgacat gtcacgactg tggctatccg
     1561 acggatgccg gggcgtacaa cccctcaacc gacaagtggc gcgccatccg tctcggtccg
     1621 atgtccggcc gcgtcgacca cacttcggtg tggaccggcc aagagatgat cgtctacgga
     1681 ggaagcaccc ctggaggtag ccgccgccgg aacagcgcag gcttgtacga cctcgctgca
     1741 gatagatgga gaaagctgcc cgagcctccg atcgcgggtc ggaacttcca tgtcgcggta
     1801 tggaccggca ccgagatgct cgtgtgggga gggcagaagc ccaacggcca gcccttcagc
     1861 gatggggtgc ttttcgttcc tgattggtaa tgccatccga ggctgtttgg gacgcggcca
     1921 gctccgaata gagatcacct gcagcaacaa gttcgtcgtg agtgccctgt tctacgaacc
     1981 tcccgtcttc catcacgact atgaggtcgg ctctggcgat ggtgctgatc cggtgcgcga
     2041 tcacgatccg agtgcagtcg agtcgctgca ggttctgata gatggccttc tcggtgactg
     2101 cgtcgagcga gctcgtcgcc tcgtcgagga gcaggatcgc cggcgcgtgc accagagcgc
     2161 gggcaagcgc gatcctttgt cgctgcccgc ccgatatcga cgcgccgccg tcggacagga
     2221 ccgtgtcgta gcccatcggc atcgccatga tgtcgtcgtg gatgcaggcg agacgggcgg
     2281 cccgctcgat ctcttcgaag gtcgcctcgg gcttggtcaa cgagatgttc gtttgaacac
     2341 tggtgccgaa taaatacgag ttctgaggaa cgatcccgag ctgcgcccgc acggtcgttg
     2401 cctccagctc ggccagatcg cggccgtcgt agaggaccgc tcccctcgtc ggcttgtaga
     2461 ggctgaggat gaggtgggca agggtcgact tgcccgaacc cgatcggccg acgacgccga
     2521 ccatcttccc gggctcgatc tcgagggaga cgccttgaac gacttctggc gcggtctccg
     2581 cgtaccggaa gccgacttcg ttcagcgtga tgcggccccc gaggcgcccg gccggctcga
     2641 cctcggccgg gttctgctcg ggggccgtgt cgagaacgtc gttgatgcgc tccatgtagc
     2701 tgcccaagag ctgtagttgc gttccggtga tgatcaggct cgacaacggc gtaaggaagc
     2761 cggtcgccat cgcgctgaga gcgagcatcg ttcccagcgt cagatcccca ctaaggaccc
     2821 cgagtgcacc aacggtcaga acgatcaaag gcgacgcaac ccgaagtgag gtcgtcaacg
     2881 tctcgatcgt ggcgctcagc cgcccacgcg caagggtcgc gttgatctcg tcgacgaaca
     2941 ggttcgacca ttgcgcaacg ccgcgcgact cggcccccac cgatttgagc gtctcgatcc
     3001 cggtgacgag ctgaccgagg tagcccgccg agcgcgcctg ggcctgcaag gtctcggcca
     3061 tcacacgttg catcggtcga cgggtgaagg cgaggatcgc gacctgcaag acggccagcg
     3121 caacgacgac ggcacccatc acaacgctct gcgcaaagat cagcagcagg tagatgctta
     3181 cgagtgctcc atcgagaacg gacgagatcg cgcctgtcgt caggatctct cgcacggtga
     3241 tgttggagtt caaccgcgcc atcaggtccc cggcggatcg ctgcaggaag aattggtacg
     3301 gcagcttgac gagatgcccg atgaaaccgg tcgagatgtt catgtcgagc tgagccctca
     3361 gtgcgagcaa gaggtgactc cgcacgtagg tcgacagcag gtagaacccg accatcgcca
     3421 gcatcccaag cccgaccgcg agtagtagat cgcggtcgcc gggagggggc accacccgat
     3481 ccacgacgac ccccatcagg atcggtgtgg ccaacgcgaa cagctggatc aacaacgaag
     3541 taaccccaac cttggagagg agccgtgatt gggtcagcat cggcttcagg tagcgccacg
     3601 ctccacccga cgctcccccg cgttcgaact cggtcgtcgg tttgaggacg atcgcgacac
     3661 cggtgaacga cttggacacc ttctccaacg acagtcgcac acggccgagg gcgggatcga
     3721 gaacctcgac acctttccgg gttactcggt cgagcacaac gaagtggttg aactcccagt
     3781 gaaggatcga gccgcgctcc aggtacttga gaccctcgag ctccacgctc acgccgcggg
     3841 cgtccatccc gtacgcccgc gctgcctcga cgagagagcg cgcgctgacg ccgtcgcgcg
     3901 acgtccgggt cgtgcgacga acatcctccg ggctcacgtt gcgtccgtgg aaggcgagat
     3961 tcatcgcaag gcacgcggca ccgcaatcgc tgatctctag ttgttggacg tacgggatcc
     4021 gcctaggcat cgtcgtctcc gaacagcgcg cgtagtcccg ggaccagggc cacgatcacc
     4081 gggtccgaac ccacctccac gcgagcgatc cccgttgcgg acgccgtagc ccgcgcggag
     4141 gtgacgatca gcacggaggg ctcatcgacc tcgacgccga agcgtctctc gacgagagcg
     4201 gtggacatcg gccggcctcc cgcggcgatg acccgggcct cccggccccc gatcagcacg
     4261 gagcttcctt taacaacctc ggcggcggcc ggtcccggca ctagcaccgc gacccgccta
     4321 ccaagccggg acgtcggtcc ggtggcgaag gaacggatct cgatctgggt ggcggctatc
     4381 agggcaacgg ccactagggc gacgaggccc cagaaggccc aggtggtcca ggtagccccc
     4441 aatcggacga cgtcgccggg ggtctgaacc cgggcgcgag cctccagggc ttcgggcctg
     4501 aatatcgagg atccgtcggt cattggggag gagtcctatc ccaccatcgg catggacttg
     4561 ccagcaagcc gagctttgcg gcaggatacc cgtcgataac ggctgaaaag gaggcgaatt
     4621 ggacacgacg ctgacgtcgg atggtccgaa gctgcgggag ctcttcatcg aagagctggc
     4681 ggaggtccgg gcgggttcat cgcaaggcgg cggaaacacc acgatggcat gttgcgagga
     4741 aggcccgttc ggctgctgcg acatccttcc gatcgacccg atcgactaag tctttctggt
     4801 ccctggcagg actctgccgg gtactcgcag aaagacctgg ttacgtgatc tgtccggtct
     4861 g
//
LOCUS       DATMED010000423         2910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5714091, whole genome shotgun sequence.
ACCESSION   DATMED010000423 DATMED010000000
VERSION     DATMED010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5714091"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VNP73_11090"
     CDS             <1..838
                     /locus_tag="VNP73_11090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL66509.1"
                     /translation="RRIGMTPSWMAGSLRKRIYGFIIRRAAAVVAVAETAARQLVDEF
                     GVPSSSIELIGNAVDEQRVRSSADPQDVKRRLHLDEEAPAVLFAGALTDEKNPLELIE
                     IERRVRAVLPRVSFLIAGDGPLAGDLDSAVSGAGVRIKVLGSRSDLPDLMRACAAVVM
                     TSRTEGIPGVAIEAGLSRRPVVAYGVGGLPEVVTHGHTGLLAPHGDAHALSDLLIQVL
                     NDPTEAKALGERAQARCRELYGIEASGRLYLDLYNRLGNETEQSPLMKRDPDITLTGE
                     PR"
     gene            835..1845
                     /locus_tag="VNP73_11095"
     CDS             835..1845
                     /locus_tag="VNP73_11095"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWL66510.1"
                     /translation="MRHDVTDVDIPFVFIVGCGRSGTTMLRSMFDSHPDIAIPGESGF
                     IWRRRNRYDGGEGFDARAFVDYLLDHRRFRRWGIERSAVQSALLDPAPATYADALRIV
                     YRLYADSRDKERYGDKTPGHVLRIPLLTELFPESRIIHLVRDGRNVALSYMDIKEWGP
                     SKVAEAAQYWKRRVQRGRKDGLALGQDRYLEVTYESLVEDPENQLRRLAQFAGVAYHD
                     EMMLYFERAGEVLTTELHPHRHQGLYKPPTKGLRDWRTKMGETDIACFEAVAGNVLEE
                     FGYERRFERLPSSVRLKAGIDVGASQLRRTRKIARGAGRRVHRQLRPSATASRGATGG
                     KR"
     gene            1842..2849
                     /locus_tag="VNP73_11100"
     CDS             1842..2849
                     /locus_tag="VNP73_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66511.1"
                     /translation="MTRAERPVIACVAENNEEWFTKAYNLVLSVRKMGGRMADTPVIV
                     HFVEDVRPEYRIQIEGLGAQVRVVEPYGSRYRFSNKMRMFDLFAEESFDALVAIDCDV
                     ILTGDIVDHFRTDAISIVPAGQDHLSADQWLELYAMFEIDPPEKSCVMRVTGQRTFPY
                     YNTGVMSIPRELGPRMLEAWTRYLARMDRVHEVWPNVVHENQIAFALAIADEALPIRE
                     LPVSLNLSAGVPPAREFNNEVEEPYVVHYHKAIDDRGFILASSIRRANGAIDAFNRLR
                     AKELGLGYEGLERVPLLKRMQKSAATRSWYNARSLKRLRRSILGSAAKKVGAGTRRTS
                     V"
BASE COUNT          588 a          842 c          933 g          547 t
ORIGIN      
        1 ccggcgaatc ggcatgactc cctcttggat ggcgggatct ctccgcaaac gaatctacgg
       61 attcatcatt cgtcgcgctg cggcggttgt ggccgtggcc gagacggctg cccgtcagtt
      121 ggtcgacgag ttcggtgtac caagctcgtc aatcgagctg atcggcaatg ctgttgacga
      181 gcagcgcgtt cgaagctcgg cggatccaca agatgtgaag agacggctcc atctggacga
      241 ggaggctccc gccgtgttgt ttgccggagc cctcacggat gagaagaacc ctctcgagtt
      301 gatcgagatc gagaggcgcg ttcgcgcggt gctcccgcgg gtgagcttct tgatcgccgg
      361 ggacggcccc ctcgccggtg acctggattc ggcggtatcc ggggccggtg tgagaatcaa
      421 ggtgctcgga tcgagatcgg atctgcccga tctgatgcgc gcttgcgcgg ccgtagtcat
      481 gacgagtcgc accgagggca taccgggcgt tgcgatcgaa gccggtttgt cccgtcgccc
      541 ggtcgtggcc tatggagtcg gaggacttcc cgaggtcgtg actcacgggc acacagggct
      601 tcttgcaccc cacggagacg cgcatgcgtt gtccgacctg ttgattcaag tgctgaacga
      661 ccccactgag gcaaaggcgc taggcgaaag ggcgcaggct cgctgccgcg agctttatgg
      721 gatagaggca agcgggaggc tctatctcga cctctacaac cgcctcggga atgagacaga
      781 acagtcgccg ctcatgaaac gagatcccga catcaccttg acaggagaac ctagatgaga
      841 catgacgtca ccgacgtcga cattccgttc gttttcattg tcggttgcgg caggtccggc
      901 acgaccatgt tgcgctcgat gttcgactca catcccgata tcgccattcc gggagagtct
      961 gggttcatct ggcggcgccg aaaccgttac gacggtggcg agggttttga cgcgcgagca
     1021 ttcgtcgact acctcttgga ccaccgcaga ttcaggcgtt ggggcattga gcgttcggcc
     1081 gtccaaagcg ccctgctcga tcccgctccg gcgacctatg cagacgccct gcggatcgtg
     1141 tacaggctat atgcggacag ccgagacaaa gagcgatatg gagacaagac ccccggacac
     1201 gtgctccgaa tcccactgct gaccgaactc tttcccgagt ctcggatcat ccacctggta
     1261 cgagacggcc gcaacgtcgc cctctcttac atggacatca aggagtgggg accctcgaaa
     1321 gtcgcggagg ctgcgcagta ctggaagagg cgggtacagc gcggtcgcaa ggatggtctc
     1381 gcgttgggcc aggaccggta tctggaggtc acctacgagt ccctggtcga agaccccgag
     1441 aatcagctaa gaaggttggc gcaattcgcg ggggtcgcgt accacgacga gatgatgctg
     1501 tatttcgagc gagccggcga ggtgctcact acggagctcc acccgcaccg gcaccagggt
     1561 ctgtataagc cgcccacgaa gggattgcgg gactggcgca cgaagatggg agagacagac
     1621 atcgcttgct tcgaggcggt cgctggaaat gtcctcgagg agttcggtta cgagcggagg
     1681 ttcgagcgct tgccgtcttc cgtccgcctt aaagctggaa tcgacgtagg agcgtctcaa
     1741 ctacgccgaa ctcgaaagat cgcacgcgga gccggtcgtc gtgtgcatcg tcagcttcgg
     1801 ccctcggcca cggcgagcag gggcgcaacc ggaggcaaga gatgacccgt gccgaacgcc
     1861 cggtcatagc ctgcgtcgcg gagaacaacg aagaatggtt cacgaaggcg tacaacctcg
     1921 ttctttccgt caggaagatg ggcggacgga tggcggacac cccagtgatc gtccacttcg
     1981 tagaggatgt gcggcccgag tatcggatcc agatcgaggg gctcggcgct caggtgcgcg
     2041 tagtggagcc ctacggatcg cgctaccggt tctcgaacaa aatgcggatg ttcgatctgt
     2101 tcgcggaaga gtccttcgat gcactggtcg cgatcgactg cgacgtgatc ctcactggtg
     2161 acatcgtcga ccatttccga acggatgcca taagcatcgt ccctgcgggg caggaccatc
     2221 tgagcgccga tcaatggctc gagctgtatg cgatgttcga gattgatccc cctgagaagt
     2281 cctgtgtgat gcgcgtaacc ggccagcgga cctttcccta ctacaacacc ggtgtgatga
     2341 gcatcccccg agagctggga ccgaggatgc tcgaagcctg gacgcgctat cttgcgcgga
     2401 tggaccgcgt tcacgaagtg tggcccaacg tcgtccacga gaatcagatc gcatttgcgc
     2461 ttgcgatcgc cgacgaggcg ctgccgatca gggagcttcc cgtgtctctg aatctctcag
     2521 cgggggttcc gccagctcga gagttcaata atgaggtcga agaaccgtac gtggtgcact
     2581 accacaaggc catcgacgac cgcggcttca tccttgcctc gagcatccgg cgggcaaacg
     2641 gggcgatcga tgccttcaat cggctaaggg cgaaggagct cggcctcggc tatgagggat
     2701 tggagcgcgt gccactgctc aagaggatgc agaagagcgc tgcaacgcgc tcctggtaca
     2761 acgcccgctc cttgaagcgc cttcgtagat caatcctggg aagtgccgcc aagaaggtcg
     2821 gggcgggaac aaggaggaca tcagtatgag caccactgca ccgatcaagg tcgcctacat
     2881 catggcgtgg ggtcgaagtg gaagcacgtt
//
LOCUS       DATMED010000424         9809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4562605, whole genome shotgun sequence.
ACCESSION   DATMED010000424 DATMED010000000
VERSION     DATMED010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4562605"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VNP73_11105"
     CDS             complement(<1..670)
                     /locus_tag="VNP73_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWL66512.1"
                     /translation="MSYRDELSFAIELARIGGRIALGHYGRDPKSEKKADGTWVTEAD
                     WAAEAQIRLRIARTFPDHNILGEEEGLTQAGGGPPLEGAPTWVIDPIDGTNNFIAGIP
                     IWATLVGLQVDGSSVVGVAHAPVLGETYDGAIGHGARVNGTPIKVDAIDSLEQAMFVY
                     ADPAAFKAAGLTAFFDAMTASAWRSRGLGDFWGHMLVARGAAHVMVEPELSVWDVAAL
                     QPIVT"
     gene            complement(672..756)
                     /locus_tag="VNP73_11110"
     tRNA            complement(672..756)
                     /locus_tag="VNP73_11110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Leu,seq:tag)
     gene            complement(769..1260)
                     /locus_tag="VNP73_11115"
     CDS             complement(769..1260)
                     /locus_tag="VNP73_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66513.1"
                     /translation="MRRVVFFLSIVVLVGSFACTDDEPEILAEQALSDFGPYTFVESE
                     AEAESNLVEYMESVTPTGAHIVRVVVRSVRSESSEVSVEAVAATFHLADLETGPFQAK
                     IEKRLAPGAREVLEGRGRLVDLEGTAALVAFLDDTTLIYLVGRHAADLETIATGLLDP
                     NDS"
     gene            complement(1268..2656)
                     /locus_tag="VNP73_11120"
     CDS             complement(1268..2656)
                     /locus_tag="VNP73_11120"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66514.1"
                     /translation="MLSAQGLYEPNETLLTLTIAAIVGWFLIVAGIYLVRMPKRPSPG
                     PPTTELGPEPPAISNLLVNSFRATKDAVPATLLDLAARRVIDVEDTGIGRYICRLKEV
                     NERLTPHEERLLAHLQSVAARGVVPAGALTTGPQESSKRWWRGFRKDVIKLAQDAGLS
                     RDLWTKSIQGAIWTAAIGIFLLFEVAIGFNDVDEVQRSGLMDAITIGHGAGLFTLIAI
                     GASTRQSDTEIGRRTASRWLGVRRALKRSPSFEVTPPTGVAMWDRYLAHAAALGVAAA
                     ALDALPMGAESDHEAWTAHGGEWRKVEVRYPRWRPGWGRHPALAVVFGAAGAYISYRL
                     LWALLGVTIDTDTLPSLLVPVRLGMVILLALLFLRWLLELGCGLSDLIASKEVTGPVL
                     RTRTRWSPVPGHGSNEKAQRYFVAIDDGRSERIDAYRVSEKKYPQFAQGLDVTLTITP
                     RLGYVRTISNRG"
     gene            2703..3362
                     /locus_tag="VNP73_11125"
     CDS             2703..3362
                     /locus_tag="VNP73_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66515.1"
                     /translation="MHWLGIAFFAGAGIVVVRWLANRTDGLGRRRPFPLFWTVALIVL
                     GCASLTPFVLRYRLENRLSAAATTMAGFDVEVYCQSFGEAFVDAGAEAGFVRFGPDGV
                     PERSTLIKRQQCRDLSSYLRSSKDAPSEPHAVAVHTLTHETIHMTGIANEGVTECHAV
                     QRDAEMARLLGASPEGGRALAAIYWNRFYPRMPDGYRSEECGPGGGLDLGGPDVPWRG
                     S"
     gene            3439..4587
                     /locus_tag="VNP73_11130"
     CDS             3439..4587
                     /locus_tag="VNP73_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWL66516.1"
                     /translation="MRRLAVVLGIILGMGCVPAMAQDAPGITLKASRSKITYLQQVRV
                     SGAISPAAADQPVEIVDQDGRVRARLTTDDQGRFATRLQPKANSTLRAQWVGAPSDAV
                     VVKVRPLVGVRLGRVRLFGHSRVRGTVKPVHEGGRVTVTLRRNGRRFTSRTLRLTDGT
                     SFSGLFGIKKPGSFRAVASFKDDDHLRASDWSTTRSTVLPRLSVGSTGAYVGVLERRL
                     DALGYLLKGVDRTYDVRTSDAVIAFNKVNRRSRLGSVDESTWAALASPKVPQPKFAKP
                     GFHIEVDQTKQVLYFVKKKRVTRIVHVSTGAGAATRDGTFTFHRRLGGTSGGGLYYPT
                     YFDGLRAIHGWPDVPTYNASHGCVRVPMWAAQWIYNRLELGDKINIYH"
     gene            complement(4584..5780)
                     /locus_tag="VNP73_11135"
     CDS             complement(4584..5780)
                     /locus_tag="VNP73_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66517.1"
                     /translation="MANRARLGSALAAVIAAIAPAITGAGPVIAEPTLEPISQVSFED
                     GSHQEFARIGGRDYAFVATGTTQGTADLRVIDVTTPERPRVVAKIPCGTYQGHIQLSH
                     DKKTLILGLDTPSLGACMPAGMMGFATVDISDPTHPRPVGYASIPRGSHSTAAHPTKP
                     IVYNGDGFPEAPGEMQIWLIKDPSRPKLVSTLATGEHSPHDLSFSHDGRMAALASVTS
                     LKLLDTSDPLNPKIEFVTQCPGCVHTHEARFTPDGTGLVVNDEFLTGPNPCPGGALYF
                     YEIVETPAGRSLELTGEYVPNQIVLDPRSQVAFCTPHVFDISDDGTKIAASWHRGGVH
                     YLDISETNGAAFGTMAPAGGVKEIASYFPDGDSFSAKFHRGPYIYLVDSRRGFEILRV
                     NGPAQP"
     gene            5867..8341
                     /locus_tag="VNP73_11140"
     CDS             5867..8341
                     /locus_tag="VNP73_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWL66518.1"
                     /translation="MRILRILVALFLLTTAVAPAASAGALDGAPIEPLVLEQAGVTGA
                     FVHARPGQLNTSITAARAAGLTVGQPYEKIGVAHVIGTAAAFKALSLSPSIEWIEANR
                     PIHMLMDSSHKATRGEEAYRGTDEVPGYDGSGVGVAVIDTGVDGTIPALQPALERGAN
                     LKILCPVPGAAWRLATQGFMGCPTDTVTVDMEDTDTIAMGGHGTHVAGIVAAAETEVV
                     PSDTSQRPFTVHGAAPGAALYGIGAGAALSVDNAMDGLEWVLDNHDSVPVPIKVINNS
                     WGTAYEDYRDPDDPANPGVFIEDGYRLHRALWKMQEQLVEEGITVVMAAGNSGGGLGP
                     APTTVAGCVNPTPGVICVANYNDFGTGSRSGVIDGSSSRGQRDRPETWPDIAAPGTNI
                     VSTCRPSLPVCATGAQINDEYFALSGTSMAAPHIAGIVAQLLEADPTLTPADIEDVLE
                     DTAHKFEWGTPYGRYTDETNADDESSFEKGHGLVDVLAAVQNVESPPVGPAGPDPIVF
                     AASGLILGQGATSFFEPTVESNSSITMHAFENTCDPTVPPTDGYVFEIPPDLADGKNL
                     IAASGGNAFGLWDFNMWLYDEACNRVGSHMGGIFGDVSGRLLENTRYVVVTNHFFGLT
                     TAGITIQPPPIVFTPESVFSGPSSDAAPIGVRVSALDESPRPDATVRFELIGSETQTW
                     EGTTDNRGIARSLQPLNVPAGEYTLRVSYVGDDPSLGGAVIERAFTVVAEDTAMTLSA
                     SGSGSKRTVAATLGDDDGDPVVGRTITFYASGAAIGTAVTDSSGVARVAVPSRYRSPS
                     QVFESSFCGDGFYLSASVGQLRCSLS"
     gene            complement(8317..8859)
                     /locus_tag="VNP73_11145"
     CDS             complement(8317..8859)
                     /locus_tag="VNP73_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007471281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWL66519.1"
                     /translation="MAITPDTKDWTFVLERTCSECGFDVRTFPREEVGAMLRDNAQRW
                     QALLADRSVRLRPTENVWSALEYGCHVRDVFRLYIERLEMMLTQDDPQYPNWDQDVTA
                     VEEAYEQQDPDEVQNELTAAATELADAFDQVEGAGWERTGLRSDGAHFTVETFARYLI
                     HDPIHHVRDVERGYERLQRS"
     gene            8918..9103
                     /locus_tag="VNP73_11150"
     CDS             8918..9103
                     /locus_tag="VNP73_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66520.1"
                     /translation="MGAGLGPSEDGTMLHADVFLPKDRKETERHPVILSIGPSFGSGG
                     SAPPTHPHPRRSSQPLR"
     gene            9360..>9809
                     /locus_tag="VNP73_11155"
     CDS             9360..>9809
                     /locus_tag="VNP73_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66521.1"
                     /translation="MKTRPRSRTVAVVAALTLIAVLAIPSPAQAAGVFTCSGVVPDWP
                     NYSGGVCQGSATGSFIDDGGVLRCVADCSFTMVFNSSTTPCLPGGSGPATLATGEIFI
                     SNGSGSFDTPFQATITATAMTILTSHPTGGGHAKIVPLTFPLPTCPFG"
BASE COUNT         1762 a         3074 c         3148 g         1825 t
ORIGIN      
        1 ccgtaacgat cggttgcagc gcggctacat cccaaacgga tagttccggt tcgaccatga
       61 cgtgcgctgc accacgcgcc accagcatgt gaccccagaa atcgccgagc ccgcgcgacc
      121 gccacgccga ggccgtcatc gcgtcgaaga aggcggtgag cccggcagcc ttgaatgcgg
      181 ctgggtcggc gtagacgaac atcgcctgct ccagcgagtc gatcgcgtcg accttgatgg
      241 gcgtgccgtt gacacgagcg ccatgtccga tggcaccgtc gtaggtttcg ccgaggacgg
      301 gcgcgtgcgc cacccccacg acgctcgatc cgtccacctg tagacccacg agtgtggccc
      361 agatcggtat cccggcgatg aagttgttgg tgccgtcgat cggatcgatc acccaggtgg
      421 gtgcgccttc gagtggtggt ccgcctccgg cctgcgtgag accttcttcc tctcccagga
      481 tgttgtggtc ggggaaggtg cgggcgatcc gcaaacggat ctgggcttcg gcggcccagt
      541 cggcctccgt cacccaggtt ccgtcggcct tcttctcgct cttggggtcg cgtccgtaat
      601 ggccgagcgc gatgcgcccg ccgatccgcg caagctcgat cgcgaacgag agctcatctc
      661 gataactcac gtgcgggcgg agggattcga accctcaagc tcttacgagc acgagtacct
      721 aaaactcgca tgtatgccag attccatcac gcccgcaagg tctcgatcct acgagtcgtt
      781 ggggtcgagg aggcccgtgg cgatcgtctc caggtcggct gcatgccggc ccaccagata
      841 gatgagcgtc gtgtcgtcga ggaatgcaac caacgccgct gtgccctcga ggtccacgag
      901 ccgaccccgg ccctcgagga cctcgcgcgc ccccggggca agtcgttttt cgatcttggc
      961 ttggaagggt cccgtctcga gatcggcgag atggaaagtc gccgcaactg cctcgaccga
     1021 tacttcggag gactcactcc gcaccgagcg gacgacgact ctgacgatat gcgcgccggt
     1081 aggcgttacg gactccatgt attcgacgag gttcgactcc gcctcggctt cggactccac
     1141 gaacgtgtag ggaccgaaat cggatagcgc ttgctcggcc aggatctcgg gttcgtcgtc
     1201 ggtgcacgcg aacgatccca ccagcacaac gatcgatagg aagaagacga cccgcctcac
     1261 gaacccatca tcctcgattg ctgatcgtgc gcacgtagcc caggcgcgga gtgatcgtca
     1321 gcgtgacgtc gagtccttgt gcgaactgcg ggtacttctt ctcggacacc cgataggcat
     1381 ctatccgctc ggatcggcca tcgtcgatcg ctacgaagta tctctgggct ttttcgtttg
     1441 agccgtggcc ggggacgggg gaccaacgtg tccgtgttct taagacggga ccggtcactt
     1501 ccttgcttgc gatgaggtcc gagagaccac aaccgagctc gagaagccaa cgcaggaaaa
     1561 gaagtgccaa caggatcacc atccccaagc ggaccggcac gagcaacgac ggcaacgtgt
     1621 ccgtatcgat cgtcactcct agaagtgccc acaggagtcg ataggagatg tacgcgcccg
     1681 cggctccaaa aaccaccgcc agcgcgggat gacgacccca tccgggacgc cagcgcggat
     1741 agcggacctc gacctttctc cattcgccac cgtgagccgt ccatgcttcg tgatcgctct
     1801 cggcccccat cggtagtgca tccagtgctg ctgctgctac accgagagcg gccgcgtgcg
     1861 cgagataccg atcccacatc gctactccgg tgggaggagt gacctcgaac gacggtgagc
     1921 gcttcaatgc ccttctcact cccagccagc gagaagccgt ccggcggccg atctcggtgt
     1981 cactctgacg cgtagatgct ccgatcgcga tgagcgtgaa gagtcccgct ccgtggccga
     2041 tcgtgatcgc atccatcagg cctgagcgtt gaacctcatc gacgtcgttg aaaccgatcg
     2101 cgacctcgaa cagaaggaag atgccaatcg ccgccgtcca tatcgctccc tggatcgact
     2161 tggtccagag atcacgcgag aggccggcgt cctgcgcgag cttgatcaca tccttacgga
     2221 aacctctcca ccagcgttta gagctctctt gggggccggt cgtaagagcg ccggccggaa
     2281 ccacgccgcg cgctgccacc gactggaggt gcgccagtag ccgctcttcg tggggagtca
     2341 gcctctcgtt gacttccttc aacctgcaga tgtagcgacc gatgccggtg tcttcaacgt
     2401 cgatcacccg gcgggcggcg aggtccagga gggtcgcggg gaccgcgtcc ttggtggcgc
     2461 ggaaactatt gacgagaagg ttcgagatcg ccggtggctc gggcccgagt tcggtcgtcg
     2521 gtggcccggg gctcggcctc ttgggcatcc tcacgagata gataccggca acgatcagga
     2581 accaccccac gatcgctgcg atcgtcaggg tcagaagcgt ttcgttcggc tcgtagagcc
     2641 cttgcgcgct gagcacgttg gaaggttatt gccgcgaccc cggctcgggg cgatactgag
     2701 ccgtgcactg gctcggcatc gctttcttcg caggtgccgg catcgtggtc gtgcgatggc
     2761 tggccaaccg tacggacggc ttgggccgca gaagaccctt ccctctgttc tggacggttg
     2821 cactgatcgt tctgggatgc gcgtccctca cccccttcgt cctgcgatat cgcctcgaga
     2881 acagattgag tgcggcggcc acgacgatgg ccggcttcga cgtcgaggtc tactgccagt
     2941 cgttcggtga agctttcgtc gatgcggggg cggaggcggg tttcgtgcgt ttcggccccg
     3001 acggggtgcc cgagcgctcg acattgatca agcggcaaca gtgtcgggat ctgtcctcct
     3061 acctgaggtc gagcaaggat gcgcctagcg agccacacgc ggtggcggtc cacacgctca
     3121 cccacgagac gatccacatg acggggatcg ccaacgaagg ggtcaccgaa tgccacgcgg
     3181 ttcaacgaga cgccgagatg gcccgcctct taggggcatc gccggaaggg ggtcgggccc
     3241 tggcagccat ctactggaac cggttctatc cccgcatgcc cgacggctat cgctcggagg
     3301 agtgtggtcc cgggggcggg ctcgacctcg gaggccccga cgtgccctgg cgaggtagtt
     3361 agccaaaggt cgttgacggc ccgccgccgg tgcgcttccc tgttgtttcg cgttcttgtt
     3421 gaagggaagg tttcctgcat gcgtcgcctg gcggttgtgc tcgggatcat cctggggatg
     3481 ggatgcgttc cggctatggc tcaggatgcg ccggggatca ccctcaaggc atcgcgatcg
     3541 aagatcacct acctccagca ggttcgggtg tccggcgcga tctctcccgc tgcggccgac
     3601 cagccggtcg agatcgtcga ccaggacgga cgcgttaggg cccggctcac gactgacgac
     3661 caggggcggt tcgcgactcg cttgcagccg aaggcgaact cgacgttgcg cgcacaatgg
     3721 gtgggcgcgc caagtgacgc ggtagttgtg aaggttcggc cgttggtggg tgttcgcctg
     3781 ggtcgggtgc ggttgttcgg gcacagtcga gttcggggga cggtcaaacc ggttcacgag
     3841 gggggccggg tcaccgtgac tcttcgtcgt aacggaagaa ggttcacctc gcgcaccctc
     3901 cgactgaccg acggcacgtc gttctcgggt ttgttcggga tcaagaaacc cggcagcttc
     3961 cgagccgttg cctccttcaa ggatgacgat catctgaggg cgagcgattg gtcgacgact
     4021 cgttcgaccg tgctgcccag actctctgtc ggttcgacgg gggcgtacgt gggtgtgctc
     4081 gaacgcaggc tggacgcact cggctatctg ttgaaggggg tcgatcgcac ctacgacgta
     4141 cgcacgtccg atgcggtcat cgccttcaac aaggtcaacc ggcgaagcag actgggaagc
     4201 gttgacgagt caacgtgggc cgctctcgct tcgcccaagg tcccgcagcc gaagttcgcg
     4261 aagccgggtt tccacatcga ggtggatcag acgaaacagg tcctttactt cgtgaagaag
     4321 aagcgagtca cgaggatcgt tcacgtttcg accggggccg gggccgcgac acgggatggg
     4381 acgttcacgt tccacaggcg gctcggcgga accagcggtg gcggcctcta ctacccgacc
     4441 tacttcgacg gattgcgcgc catccacggg tggcccgacg ttcccaccta caacgcaagt
     4501 cacgggtgcg tgcgtgtccc gatgtgggcg gcgcaatgga tctacaacag actggagctg
     4561 ggcgacaaga tcaacatcta ccactaaggc tgtgcgggtc cgttcactcg aaggatctcg
     4621 aagcctcgtc tcgagtcgac cagatagatg tacggccccc gatggaactt ggcactgaac
     4681 gagtcgccgt cggggaagta agaggcgatc tctttgactc cacccgccgg agccatggtg
     4741 ccgaaggcgg ctccattggt ttcagagatg tcgaggtaat ggaccccgcc gcggtgccag
     4801 gacgcagcga tcttggttcc atcgtcggag atgtcgaaga catgcggggt gcagaaggcc
     4861 acctgggatc tcgggtcgag cacgatctga ttcgggacgt actcaccggt tagctcaagg
     4921 ctgcgacctg ctggggtctc gacgatctcg tagaagtaca gcgcgccacc gggacacggg
     4981 ttgggaccgg tcaggaattc gtcgttcact accagtccag tcccgtcggg tgtgaagcgg
     5041 gcctcgtgcg tgtgtacgca gcccggacat tgcgtcacga actcgatctt ggggttgagg
     5101 ggatccgaag tgtcgagcaa cttgagagag gtgaccgatg cgagagcagc catccttcca
     5161 tcgtggctga aggaaaggtc gtgcgggctg tgctcgcccg ttgcgagggt cgagacgagc
     5221 ttgggacgcg acggatcctt gatcagccat atctgcatct caccgggcgc ctccgggaat
     5281 ccgtcgccgt tgtagacgat cggcttcgtc gggtgcgcgg cagtggagtg cgatcccctc
     5341 gggatcgatg cgtatccgac tggtcgagga tgcgtcgggt cactgatgtc gacagtcgcg
     5401 aagcccatca ttcctgccgg catgcaagcg cccagtgacg gggtatcgag cccgaggatc
     5461 aatgtcttct tgtcgtgact caactggatg tgaccctggt acgtgccgca ggggatcttc
     5521 gccacgactc gggggcgttc gggtgtcgtt acgtcgatga ctcggaggtc cgcggtgccc
     5581 tgggtcgtgc cggtggccac gaaggcgtag tcccggcccc cgatgcgcgc gaactcttgg
     5641 tgggacccat cttcgaagga gacctgagag atgggttcga gcgtcggttc ggcgatcacg
     5701 ggaccggccc ccgtgatcgc gggcgcaatg gcggcaatga ccgccgcgag cgccgatccc
     5761 aagcgggcgc gattcgccat gtcactacaa gggttcgctg cagggcagga gaggcctgcg
     5821 ccatggcgaa aagggaaaac ctaaccaagc aaatcaagga gcccctttgc gcatcctccg
     5881 gatcctcgtc gccctctttc tgctgacgac cgccgtcgcc ccggctgcat ctgccggcgc
     5941 gctcgacggc gcgcccatcg agccactcgt cctcgagcag gccggcgtga ccggtgcctt
     6001 cgtgcacgct cgacccggcc aactgaacac ttcgatcacg gcggcacggg ctgccggtct
     6061 gacggtgggg cagccctacg aaaagatcgg cgtggcgcat gtcatcggca cggcggctgc
     6121 cttcaaggct ctctcgttgt ccccctcgat cgaatggatc gaagcgaacc ggcccatcca
     6181 catgctcatg gattcgtccc acaaggcgac caggggagag gaggcctacc gcggtaccga
     6241 cgaggttccc ggttacgacg gcagcggtgt cggggttgct gtgatcgaca cgggagtgga
     6301 tggaaccatt ccggcactac agccggctct ggaacgaggg gcgaacctca agatcctgtg
     6361 ccctgtcccc ggagccgcat ggcgtctcgc gacccagggc ttcatgggtt gcccgaccga
     6421 cacggtcacc gtcgacatgg aagacacaga cacgatcgcc atgggcggtc acggaactca
     6481 cgtcgcaggg atcgtcgctg ccgcggagac agaggtcgtt ccgagcgaca ctagtcagcg
     6541 gccgttcacc gtgcacggcg cggcacccgg cgcggctctt tacgggatcg gcgcgggagc
     6601 cgcactcagc gtggataacg cgatggacgg gcttgagtgg gtgctcgaca atcacgactc
     6661 ggttcccgtt ccgatcaaag tcatcaacaa ctcttgggga accgcttacg aggactaccg
     6721 ggatcccgac gatcccgcca accccggtgt cttcatagag gacggctatc ggttgcatcg
     6781 ggcgctgtgg aagatgcagg agcaactggt cgaagagggc atcacggtgg tcatggcggc
     6841 aggcaactcg ggaggcgggc tcgggccggc tccgacgacg gtggcgggct gcgtcaatcc
     6901 gacgcccggt gtcatctgtg tagcgaacta caacgacttt ggaacgggga gcaggtccgg
     6961 tgtgatcgat ggttcctctt cacgcggaca gcgcgatcgg ccggagacgt ggccggacat
     7021 cgcggctccg ggaacgaaca tcgtttcgac ttgccggccg agtcttccgg tgtgcgcaac
     7081 gggtgcacag atcaacgacg agtatttcgc cctctcgggc acatctatgg cagcgccgca
     7141 catcgccggg atcgtggcgc aactcctaga ggcggatccg acactgactc cggcagacat
     7201 cgaggatgta ctcgaggaca cggcgcacaa gttcgagtgg ggtacgcctt acggccgtta
     7261 cacggatgag acgaatgcag acgacgagtc ctctttcgag aagggccacg gactcgtcga
     7321 tgtgcttgcc gcggtccaaa acgtggagtc gcctcctgtt ggaccggccg gaccggaccc
     7381 gatcgtcttc gcggcgtccg gcctgatcct cggccagggc gcgacatcat tcttcgagcc
     7441 aacggtcgaa tcgaactcga gcatcacgat gcacgccttc gagaacacct gcgacccgac
     7501 cgtgcctcct acagacggct acgtgttcga gatcccacct gatctcgccg acgggaagaa
     7561 cctcatcgcg gcgagcggtg gtaatgcgtt cggtctgtgg gacttcaaca tgtggcttta
     7621 cgacgaggct tgtaaccgag ttggttcaca catgggtggc atctttggcg acgtctccgg
     7681 ccgtctgctc gagaacaccc gttacgtggt tgttacgaac catttctttg gactaactac
     7741 cgcgggcatc acgatccaac cgcccccgat cgtatttacc ccggagagtg tgttttccgg
     7801 tccgtcatcc gacgcggccc caatcggagt tcgggtttca gccttggacg agagccctcg
     7861 tcccgacgcc accgttcgct ttgagttgat cggtagcgag acgcagacgt gggaggggac
     7921 gaccgacaac cgagggatag cgcgttcgct tcagcccctg aacgtgccgg ccggcgagta
     7981 caccctgcga gtgtcctacg tgggagacga tccttctctg ggcggcgccg tgatcgaacg
     8041 cgccttcacc gtcgtggccg aagatacggc gatgacgctg tctgcttctg gcagcggttc
     8101 caagcggact gttgcggcga cgctgggcga tgacgacggc gatccggtcg tcggacgcac
     8161 catcacgttc tatgcctcgg gcgccgcgat aggaacggcc gtcaccgact ccagcggagt
     8221 cgcacgcgtg gcggttccgt cgcgctaccg gagcccaagc caggtgttcg aatcaagctt
     8281 ctgcggcgac ggcttctatc tcagtgcttc cgtgggtcag ctgcgctgca gcctttcgta
     8341 gccccgttcg acgtcccgga cgtgatggat cgggtcgtgg atcaggtagc gggcgaacgt
     8401 ttcgactgtg aagtgggctc cgtcacttcg cagaccggtc cgttcccatc cagcaccttc
     8461 gacttggtcg aaagcgtcgg ccagctccgt tgctgcggcc gtaagttcgt tctgaacctc
     8521 gtccggatcc tgctgctcgt atgcctcttc gaccgcggtg acgtcctgat cccagttggg
     8581 gtactgagga tcgtcctggg tgagcatcat ctccagccgc tctatgtaaa ggcggaagac
     8641 gtcgcggacg tgacacccgt actccagggc agaccacacg ttctcggtcg gtcgcaggcg
     8701 gacgctccga tcggcgagga gtgcctgcca ccgctgcgcg ttgtcccgga gcatcgcccc
     8761 gacctcctca cgggggaagg ttcgaacgtc gaacccacac tcagaacagg ttcgctcgag
     8821 gacaaaggtc caatccttgg tatccggcgt gatggccatg gggcctatat aacccggggc
     8881 aggggtggga ggcccacggc gaatatctgc tcgccacgtg ggagcaggtc tgggcccgtc
     8941 cgaagacggg acgatgctcc acgccgacgt cttccttccc aaagaccgca aggagaccga
     9001 acgccacccg gtgatcctct cgatcggccc gtccttcggc tccggtggat ccgctcctcc
     9061 cacccaccca cacccgcgaa ggtccagtca gccgcttcgg tgatctgatc gaagagggcc
     9121 gctccgctac ctccgccgga acaatctcgc cggacctttg ccgcggccat gagcgatgtg
     9181 cccaaggcga ccatcgacga ggcactgatc gcggacaaca ccgtgccgat gaagttcccg
     9241 ccccgcctcc aacgccgcta ggcgacctga acggactcac ccgaaggtgt gaaggaaagc
     9301 aagcgcggcc caaagaatct agttccaaag gtttctgaca tgaccgattt ggaggggcta
     9361 tgaagaccag accaagatcg aggacggttg ccgttgtggc ggcgctaacg ctgatcgcgg
     9421 tcctggcgat ccccagcccc gcccaggcgg cgggggtctt cacctgttcc ggcgtcgtcc
     9481 ccgactggcc caactactcc ggtggggttt gtcaggggtc ggccaccggt agcttcatcg
     9541 acgacggtgg ggtgttgcgc tgcgtcgccg actgcagctt cacgatggtg ttcaactcgt
     9601 cgaccactcc atgtctcccg ggtggatctg ggccggcgac gctcgccacc ggagagatat
     9661 ttatctccaa cggctctgga tcattcgaca cgccttttca agcaacgatc acggcgactg
     9721 ccatgacgat cctgacctca catcccactg gagggggtca cgcgaagatc gtgccgctca
     9781 cgttccccct gccaacatgc ccgtttggg
//
LOCUS       DATMED010000425         6089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4562635, whole genome shotgun sequence.
ACCESSION   DATMED010000425 DATMED010000000
VERSION     DATMED010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4562635"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VNP73_11160"
     CDS             complement(<1..748)
                     /locus_tag="VNP73_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HWL66522.1"
                     /translation="MAVVAIEFDVAIVGGGPSGSAAAHYLASKGHSVLVCEKKSFPRE
                     KTCGDGLTPRAVKVLQEMGLGGELETWERVRGLRVHGGGRSLELPFPELEDWCDYGLV
                     KPRKDLDQIVLNNAEAAGAKVLYETQAKEPIFDGGRLIGLRAKRGSDEEEVRAKFTIC
                     AEGSATKFTRSLGRERNEGYPMGFAIRQYFKSPLQQSGWFDAYLSIVVEGQALPGYGW
                     VFPVGDGTVNVGVGLLSTYGGWRDVNLHDLQ"
     gene            complement(847..1830)
                     /locus_tag="VNP73_11165"
     CDS             complement(847..1830)
                     /locus_tag="VNP73_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWL66523.1"
                     /translation="MADPEQIYTEVLQEEQGKGVSPAVAEGRAKAARARAEQGSPHPK
                     EPKWWPGAQRHLEGGNGAAPAAEEPVEETAEEPEAPAAAEPAAEAPAEEAPTTPEPEA
                     PAAAAPPAPAEAPAASAAPAAAATPTSVAPEQRPAGVTHGVPTGTRLRPEDAVGTDAQ
                     FEGQQLVNERRKRIDEVVAAGGPPPATGPRSGQWALFLAYLAIVAFAIFFVVQNQPAG
                     GGEGGGAPPEEPAGGGGTTVTAQNVVFDTEAITLAADEEASLEFVNDDASSVQHNVAI
                     YEDDSAEKSLFQGEVIPGGQAITYDVPPLKKGSYYFQCDVHPGMNGEVTVE"
     gene            complement(1926..2936)
                     /locus_tag="VNP73_11170"
     CDS             complement(1926..2936)
                     /locus_tag="VNP73_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWL66524.1"
                     /translation="MVAVSPSPSELRDQIDRWLEGFLADRSQAVPDAGDLIAEIARVV
                     RLGGKRLRPTFCYWGYRATGHSHDDRIVAAAASLELLHTFALVHDDIMDSALVRRGEK
                     TTVAAHGSDIALLTGDLALVLADEGFMTSGFDAETLARGFGVYSRMRHEVIAGQYLEL
                     VTSTKEAPSEEEARRVAALKSGFYSVAEPVTIGAVLGGASETIVDSVRAYGLPLGEAF
                     QLRDDLLGTFGDPDVVGKPVDSDIREGKRHLLFVKALARASDDDRAVLLRDWGRADLS
                     PDEVEHLRSILVGSGAVTDIEALIQRLASAAREALDSVEIDTQARAALGSLIDSVVSR
                     ES"
     gene            2974..3930
                     /locus_tag="VNP73_11175"
     CDS             2974..3930
                     /locus_tag="VNP73_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWL66525.1"
                     /translation="MTSFKRVALAAAGATFLLVSIGGLVRATKSGLGCGTDWPHCSGR
                     LLPELANRAMVIEYSHRAVAGIVMVLMGALAYLAYRKGLPQRIKRAAVGAFALVLFQA
                     ILGMIVVVLELQAVSVVLHLGTAMALLALVLYIALTTDDRGVATDARTARRATRVAVG
                     VFLLLLVGSYMSGAGAGYVFPDWPLMDGRVIPDLAVEGKALHFLHRVLAAIVGLVVLH
                     NAIATSRSERKSRASVALAHGALALFTIEALIGAANVFTGGNDAFVTLHLAMGAGIWA
                     TLVSQAIVTRAAANESVAETSRSAEPALGSARDRLGERAGAS"
     gene            3930..4874
                     /locus_tag="VNP73_11180"
     CDS             3930..4874
                     /locus_tag="VNP73_11180"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWL66526.1"
                     /translation="MARRLTAEASGALRIEESVDSPAQASLRDRIAAYVALTKPRIIE
                     LLLVTTVPSMIVAAEGWPGLSLVLWTLLAGGLSAGGANAINCYVDRDIDAVMTRTRKR
                     PLPTHKIAPGRALVFGTVLGVVAFLLMWGTVNLPAAFLSLFALLFYVFVYTLGMKRST
                     PQNIVIGGAAGAMPALVGWAAVTGTIELPALALFGIIFYWTPPHFWALAMRYEKEYAR
                     ADVPMMPVVYGRKETVRHILLYSLMLFAMCLAFFSVAKMGIIYLIAAVALNAMFVGWA
                     VKLWRNTSTRTAWGLFRFSIYYLALLFMAAAADVLIRA"
     gene            complement(4878..5519)
                     /locus_tag="VNP73_11185"
     CDS             complement(4878..5519)
                     /locus_tag="VNP73_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWL66527.1"
                     /translation="MSQQVDPLQGEVRFRVPLPIVIPIGALLLIAVVTIIVSRILLSV
                     PKEVSVVIAMALAANVLIACAVLALRPQETRNSWAELLVVATYPLVIGIVLANLGLGE
                     SHAGAAHGAAEEAGVTGGGLEIGAENVQFTMDTIELPADEAATLEFTNADASSVQHNV
                     SIYKDDSAEKVLLQGEVIPGGQSISYEIDPLKKGEYYFQCDVHPGMNGTVTVE"
     gene            complement(5516..>6089)
                     /locus_tag="VNP73_11190"
     CDS             complement(5516..>6089)
                     /locus_tag="VNP73_11190"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWL66528.1"
                     /translation="LLMGLPASVLRRLFRPRALQLVLRFVTRPLVALIIFNGVVLVTH
                     WPVLVDASLRSELLHFSMHTILFTSAVIMWWPVIDPLPETKKLSEPGKMFYLFLQSIL
                     PTVPASFLTFADAPIYHFYETVPRLWGISVVNDQQIAGLIMKIGGGLLLWLCIATIYF
                     RWSAKEERGESDEISWEDFERELEVWDLKT"
BASE COUNT         1047 a         1988 c         1917 g         1137 t
ORIGIN      
        1 tctgaaggtc gtgaaggttg acgtcgcgcc accctccata cgtcgagagc aaaccgactc
       61 cgacgttgac ggttccatcg cctacaggaa agacccatcc gtagcccggg agcgcttgtc
      121 cttccacgac gatgctgagg taggcgtcga accatcccga ttgctgcagg ggcgacttga
      181 agtactggcg gatcgcgaag cccatcggat agccctcgtt gcgttccctg ccgagcgacc
      241 gggtgaactt cgtggccgag ccttcggcac agatcgtgaa cttggcacga acctcttcct
      301 cgtcgctccc gcgcttggcg cgcaacccga tgagccgacc gccgtcgaag atcggctcct
      361 tggcctgcgt ttcgtaaagg accttggccc ccgcggcctc tgcgttgttg agaacgatct
      421 ggtcgagatc cttgcgtggc ttcacgaggc cgtagtcaca ccagtcctcg agctcgggga
      481 atgggagttc cagcgaccgg cccccaccat gaacccgcag cccgcgcacg cgctcccacg
      541 tttcgagctc gccacccaag cccatctctt gcagcacttt gaccgcccgg ggagtgaggc
      601 cgtcaccgca cgtcttttcg cgcgggaacg acttcttctc gcacacgagc accgagtgac
      661 ctttggaggc gaggtagtga gcggccgccg acccggaggg ccctccgcca acgatggcga
      721 cgtcgaattc gatcgctacg accgccacct ccttccgagg acccgagggt cccgtagaac
      781 gcttgtgaaa actttcacga gcgcggatgc gcagtctatt actccggcct cgggccggac
      841 gaagtgttac tcgaccgtga cctctccgtt catcccggga tggacgtcac actggaagta
      901 atacgagccc ttcttgagcg ggggcacgtc gtaagtgatc gcttgccccc cggggatcac
      961 ttcgccttgg aagagagact tctctgcgct gtcgtcctcg tagatcgcga cgttgtgctg
     1021 gacggaagag gcatcgtcgt tgacgaattc gagacttgcc tcctcgtcgg ccgcaagcgt
     1081 gatggcttcg gtgtcgaaca ccacgttctg agccgtcacg gtggtgcccc ctccacccgc
     1141 aggttcctcg ggtggagcac ccccgccctc gccgcctccg gcaggctggt tctgaacgac
     1201 gaagaagatc gcgaacgcaa cgatggccag gtatgcgagg aacaaagccc actgaccgct
     1261 gcggggcccc gttgcggggg gtggaccccc ggcggcaacg acctcatcga tcctcttgcg
     1321 gcgctcattg accagctgtt ggccctcgaa ctgcgcgtcg gtccccaccg cgtcctcggg
     1381 tcgcagcctc gttcccgtgg gaacgccgtg ggtgaccccg gcaggacgct gctcgggagc
     1441 gactgaagtg ggagttgcag ctgcagccgg tgccgcggac gctgcgggag cctccgcagg
     1501 agccggtgga gctgcggcgg ccggagcctc gggctcgggc gtcgtgggag cttcttcagc
     1561 gggtgcttcg gcggccggct cggccgcggc cggggcctcg ggctcttcgg cggtttcttc
     1621 gaccggttct tcggccgcgg gggccgcgcc gttgccgcct tcgagatgcc gctgcgcgcc
     1681 gggccaccac ttcggctcct tgggatgggg cgatccttgt tcggctcgcg cccgtgccgc
     1741 cttagcgcga ccctcggcta cggccgggga tacacccttg ccttgctctt cttgaagaac
     1801 ctctgtgtag atctgttccg gatcggccac tcacgactcc tggactggga tgggtcgggc
     1861 gcactttacc cgcgggacca catccattca tctcaggcga gcactagcgc gcaagccggt
     1921 gcctcttacg actcccggct cacaaccgag tcgatcaggg aaccgagtgc ggctcgggcc
     1981 tgcgtgtcga tctcgaccga gtccaacgcc tctcgtgcgg ccgaggccag ccgttggatc
     2041 agcgcctcga tgtcggtcac cgctcccgat ccgaccaaga tcgacctcag gtgctcgacc
     2101 tcgtcgggag agaggtcggc ccgaccccaa tcccgcagca ggaccgctcg gtcgtcgtcg
     2161 gatgcgcgcg ctagggcctt gacgaatagg aggtgtcgct tgccttcccg gatatcggaa
     2221 tcgaccggct tgcccacgac atcgggatcc ccgaaagtgc cgagtaggtc gtcccgcagt
     2281 tggaacgcct cgcccaacgg aagtccatag gcgcgaacgc tgtcgacgat ggtctccgaa
     2341 gcaccgccga gcacggcacc gatcgtcacc ggttcggcga ccgaatagaa ccccgacttc
     2401 agcgcagcga cgcggcgggc ttcttcctcc gacggagcct ccttggtgct ggtgacgagc
     2461 tcgaggtact ggcctgcgat gacctcatga cgcatccgcg agtacacccc gaacccgcgc
     2521 gccagcgtct cggcatcgaa cccggatgtc atgaagccct cgtcggcaag gacgagggcc
     2581 aggtcgccgg tgagcaacgc gatatcggaa ccgtgcgcgg ccacggtcgt cttctcgccc
     2641 cggcgcacga gggcggaatc catgatgtcg tcgtggacga gagcgaacgt gtgcagcagc
     2701 tcgagcgaag cggcggcagc cacgatgcgg tcgtcatgcg agtgccctgt ggcccgatag
     2761 ccccagtaac agaacgttgg tcgtaagcgt tttcctccga gccggacgac gcgagcgatc
     2821 tccgctatca ggtcgcccgc atcggggacg gcttgagatc ggtcggccag gaagccttcg
     2881 agccagcgat cgatctggtc ccgaagctcg ctcggtgaag gtgatacggc caccatgccc
     2941 atcacactag tggcacggtt aacatccgcc tcgatgactt cgttcaaacg agtcgcgctt
     3001 gcggcggcgg gcgccacgtt cctgttggtc tcgatagggg gcctggtacg tgcgaccaag
     3061 tccggcctcg ggtgcggcac cgattggcca cattgttccg ggcgactgct gcccgagcta
     3121 gcgaaccgcg ccatggtgat cgagtactcg caccgcgccg tggcggggat cgtcatggtg
     3181 ctgatggggg cgctggccta cctcgcctac cggaaggggc tgccccaacg catcaagagg
     3241 gccgccgtgg gcgcgttcgc cctcgttctg ttccaggcga tcctcgggat gatcgttgtc
     3301 gtcctcgagc tgcaggcggt ctcggtagtc ctccatctcg gcacggcgat ggccctgctt
     3361 gcgctggtcc tgtacatcgc gctgaccacc gacgaccggg gcgtcgcgac cgacgcgagg
     3421 accgctcgaa gagcgacacg ggtcgcagtc ggagtgttct tgctattgct cgtggggtct
     3481 tacatgtccg gggccggagc cggttatgtc ttccccgact ggccgttgat ggacggtcga
     3541 gtgatccccg atctggccgt cgaaggaaag gcccttcact tcctgcaccg cgtcctggca
     3601 gcgatcgtcg gcctcgtcgt cttgcacaac gcgatcgcaa catcgagaag cgaacggaag
     3661 tcgcgcgcct cggtggccct cgctcacgga gccctggctt tgttcacgat cgaagcgctg
     3721 atcggagcgg caaacgtgtt caccggagga aacgacgcgt tcgtcaccct tcacctcgcc
     3781 atgggggccg ggatctgggc aacgctcgtg tcgcaagcga tcgtgacccg ggcggcggct
     3841 aacgagtcgg tggccgagac gagcagatcg gccgagcccg cgctggggag cgcgcgcgat
     3901 cgcctcggcg agcgcgcagg agcaagctga tggcaagacg attgacggcc gaagcctccg
     3961 gcgctctgcg catcgaggag agcgtcgatt cccccgcaca agcatcactg cgcgacagga
     4021 tcgctgcata cgttgccctg acgaagccac gcatcatcga actcctgctc gtcaccaccg
     4081 ttccctcgat gatcgtcgcg gcggagggct ggccggggtt gagtctcgtg ctgtggacgc
     4141 ttctggccgg aggcctgtct gccggaggcg cgaacgcgat caactgctat gtagaccggg
     4201 acatcgatgc agtgatgacc cgcacgcgga agcgaccgct accgacgcac aagatcgcac
     4261 cggggcgggc gctcgtcttt ggaaccgtcc tcggcgtggt cgctttcctg ctcatgtggg
     4321 gcaccgtcaa tctccctgca gcgttccttt ccctgttcgc tctcctcttc tacgtgttcg
     4381 tctacacatt ggggatgaag cgctcgacgc cgcagaacat agttatcggc ggagcggcgg
     4441 gtgcgatgcc ggctctggtc ggttgggcgg ccgtgacggg aacgatcgag cttcccgccc
     4501 ttgcgttgtt cgggatcatc ttctactgga cgccgcctca cttctgggct ctcgcgatgc
     4561 gctacgagaa ggagtacgcg cgggccgatg taccgatgat gccggtcgtc tacggtcgta
     4621 aggagaccgt ccgtcacatc cttctctact ccctgatgtt gttcgcgatg tgtctcgcgt
     4681 tcttctccgt agcaaagatg gggatcatct acctgatcgc agcagtggcg ttgaacgcaa
     4741 tgttcgtcgg ctgggccgtc aagctgtgga ggaacacgtc cacccgcacc gcgtggggac
     4801 tgttccggtt ctcgatctat tacctggcac tgctattcat ggcagccgcc gccgacgtcc
     4861 tgatccgcgc ctgatcccta ctcgaccgtc acggttccgt tcattccggg gtggacgtcg
     4921 cactggaagt agtactcacc cttcttcaac ggatcgatct cgtacgagat cgactgaccg
     4981 ccggggatga cctctccctg gagcagcacc ttctcggcgc tgtcatcttt gtagatcgag
     5041 acgttgtgct gaacggatga agcgtcggca ttcgtgaact cgagcgtcgc ggcctcgtcg
     5101 gcaggaagct cgatcgtgtc catggtgaac tgaacgttct cggcaccgat ctcgagccct
     5161 ccgcccgtga cccccgcttc ctcggcggca ccgtgggctg ctccggcatg gctctcaccc
     5221 agtcccaggt tcgccagaac gatcccgatc acgagcggat acgtagcgac cacgagcagt
     5281 tctgcccacg agttgcgggt ctcctgagga cgcagagcca acaccgcgca cgcgatcaga
     5341 acgttggcgg ccagcgccat ggcgatgacg accgacactt ccttcgggac cgagaggagt
     5401 atccgcgaca cgatgatcgt cactacggcg atcagtagca gcgcgccgat cgggataacg
     5461 atcggtagcg gcactctgaa ccgaacttct ccttgcagcg gatcgacttg ttggctcatg
     5521 tcttcaggtc ccacacttcc agttcacgtt cgaagtcctc ccacgagatc tcgtccgact
     5581 cccctcgctc ttccttagcg ctccagcgga aatagatcgt tgcgatgcag agccacagta
     5641 gcaaaccgcc tccgatcttc atgatcagtc ctgcgatctg ctgatcgttc acgacgctga
     5701 taccccacag ccggggcacc gtttcgtaga agtgatagat cggcgcatcc gcgaacgtga
     5761 ggaacgacgc ggggaccgtc ggaaggatcg actgcaagaa gaggtagaac atcttgccgg
     5821 gctccgagag ctttttcgtc tccggcaacg gatcgatcac cggccaccac atgatgacgg
     5881 cactcgtgaa caagatcgtg tgcatcgaga agtgaaggag ttcggaacgc aacgacgcat
     5941 cgacgagcac cggccaatgc gtgacgagga cgaccccgtt gaagatgatc agagcgacga
     6001 ggggccgggt cacgaacctc agcaccagct gtaacgcgcg tgggcggaag agccggcgca
     6061 agacggacgc cggcaacccc atcagaagg
//
LOCUS       DATMED010000426         7001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3794632, whole genome shotgun sequence.
ACCESSION   DATMED010000426 DATMED010000000
VERSION     DATMED010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3794632"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1045)
                     /locus_tag="VNP73_11195"
     CDS             complement(140..1045)
                     /locus_tag="VNP73_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWL66529.1"
                     /translation="MRLPQLARAAVIAVGFVLAIPAVAAAQEQARPELDSAPAQVSFR
                     STAVVTGHMENGTGGEKVTLQKYKGGGWIKLLTRSVDEDGDVSFRLEDLTRTAEYRLK
                     SKTPDGEPTFSRGRARIQVEARVTLEASRRHVMSGRRVTLSGVALPKRSDRRVILQKR
                     VGGEWRFVTELSTADGHFTREVRMNNTGRRAFRVLFRGDENNSKAADKARVHVYDPDP
                     ATWYGPGFYGNRTACGKRLRRGTLGVAHRSLPCGTEVSLLHHGRTITVPVIDRGPYSE
                     ADWDLTEETADRLRFEGSKTVGVTR"
     gene            complement(1342..1551)
                     /locus_tag="VNP73_11200"
     CDS             complement(1342..1551)
                     /locus_tag="VNP73_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66530.1"
                     /translation="MAGWGDDPELEKLRGLIDDGWEVVEITEDPNAPGGPSDTVKLTK
                     DGATEECTSDHLAFHRYVEGLREGA"
     gene            1620..2588
                     /locus_tag="VNP73_11205"
     CDS             1620..2588
                     /locus_tag="VNP73_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWL66531.1"
                     /translation="MSEARSDFTDFQQVFSRIVDNIEKTIQGKKDVVRLALVALVAEG
                     HLLLEDVPGVGKTMLAKSLAKSLGCSWSRIQFTPDLLPSDITGVNVWDARTGEFRFKA
                     GPVFANICLGDEINRASPKTQAALLECMEERQVTVDGVTHELEAPFMVIATQNPIEHE
                     GTYPLPESQLDRFIIRLSMGYPERESELEILERHGAHEVLDDLGAVSDAAEIAGLVKL
                     ARDVHCAPALRNYIVQLTESTRGHPDIYLGASPRASLFLLRASRALAAARGRDFVVPD
                     DVQDLVVPVLAHRILLSPEAQMRGGTSDEVLMGVLERIPVPVRTQA"
     gene            2596..3888
                     /locus_tag="VNP73_11210"
     CDS             2596..3888
                     /locus_tag="VNP73_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWL66532.1"
                     /translation="MPTRRGLLIASAGVALLVTGIVFGSRAVQQLGFALIALLLIGIA
                     VVRISRHDLAVHRQIAPQRARAHHPVTITLSIENFGRGRAPLIMLEDRLPPGLAGRAR
                     FLLPSLESGGKREAPITVRPEQRGRYKVGPLEVEAADPFGLARVRSVALGTSEFVVHP
                     RIESLVMPRDSGERRSTARATTRHATGPRGEDFYTIREYVEGDDLRKIHWASTAKRAK
                     AMIRHEETPWQTRATIVLDDRRLPYGGFSEHISFERAVSAAASLVDLYARAGYGYQLI
                     GAHHPGIPSGKGEDHFNRCLDLLATIEPKGNPKQEDPLLLARLTELEARRGGEASLVI
                     VTGSLDPTVGVAIARLRRVYHQIILVAFPSHRFGTATTSARWEGERAAREVAALITRA
                     GGRVLVLGPGDRFAPAWASFSSRTATSEMRWDLKPEHV"
     gene            3861..6278
                     /locus_tag="VNP73_11215"
     CDS             3861..6278
                     /locus_tag="VNP73_11215"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWL66533.1"
                     /translation="MGSEARTRLALAGLLVVTLLAFSRVFQEGDYPGPALLGMLLAVG
                     LAQLTRRVGLVVWASALVSMFALGWYLCLIFAGNLTFYGLPTPAAIAKIGTLVVDAYE
                     KSSLDYAPVPLRPGYVLLTIAAMWLLAALGETATFRWRRPLIPAIASAAMFSFVLIVG
                     IRKGSSFLTIVYLLGLLTYLGLESAHRLGSWGRWVSGWAKRWDSEVTTGPLARKMGAS
                     CLAAALIAPFFLPALGKGLLSWRSGIGGGAGGEIDTLVSLAPKLVKQSNDVLFEVQAP
                     RPAYWRLVTLTYFDGNSWHPEPNRETLANGRVASDLPPPNPGAPLLQRYEIRGLEGEP
                     LPAVPLPETVTMTGDDADVAMEFNPITADVEVADSVEEGMTYEVRSLVPNVTFRSMRR
                     ATIPQVKDVAESLGLPDYLPMNTYEVPRDFCPPEIPGNECPLQQSRIFRIAQRWTQGA
                     DRPFDKLLLLQNRFRGQFQHQLPGRSEVAESEVKPLASAEYLTRFLTETRTGYCQQFA
                     TAFAVLARMLGYPARVSVGFLPGETAIESPDQYIVRGNDAHAWPEVYFEDIGWIAFEP
                     TPRGAAPPPAYTQAQAPGAGVNPPALDPGGRAARGQQNPRGIRDTLDARPGRTAERER
                     REREELWHDSFVRLATTLGVVIALLLIVTPLAKLFLIRRRYRKARDGDALAGAAFAEF
                     EQEASDLAYPRGHAETAGAYVKRLVRSHNVSPARASRLAELFEEAIYGPAGLSDRQGS
                     EAVRLTHALKKDLWAEASWTLRAQRLFSPGVVVAELQANERPSNGDGSRPGGLARVLT
                     RSASERR"
     gene            complement(6193..6711)
                     /locus_tag="VNP73_11220"
     CDS             complement(6193..6711)
                     /locus_tag="VNP73_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWL66534.1"
                     /translation="MEGFTADQAIRFTGCTAHQLRYWDRISLVKPSVQATGGRPGVRR
                     LYSFRDLVALKVIRSLLDGGMSLQRVRRAIEYLRKRAALDEHLSEVKLVTDGRSIFKL
                     ARNDGEILDALREGQMAFFLAIDDIARSIDGKVAEYLYDREEFIAALRRIESELEQDL
                     PVETRRRLRAAR"
BASE COUNT         1192 a         2315 c         2192 g         1302 t
ORIGIN      
        1 attcaagacg agcgctcttc ccaaggtggg ctgagtatat agagcctgcc ctgttaccag
       61 gcctcaattc aggccggcga cggccgaggc cgtcgcctgt cgtgtccggc gcaacggtgg
      121 ccagcgcgac ggtggccggc tagcgcgtca ctccgaccgt cttggagcct tcgaaccgga
      181 gccggtcggc agtctcctcc gtgaggtccc aatcggcctc cgagtacggc ccccgatcga
      241 tcacggggac ggtgatcgtt cgtccgtggt gcaggagact cacttcggtg ccgcacggaa
      301 gcgagcgatg ggcgacgccg agcgtccctc tgcgcaagcg cttgccgcac gccgttcggt
      361 tcccgtagaa accgggcccg taccaggtcg ccgggtccgg gtcgtagacg tgcaccctcg
      421 ccttgtccgc ggccttgctg ttgttctcgt cacctctgaa gagaacgcgg aaggcacgcc
      481 ggccggtgtt gttcatgcgg acttcgcgtg tgaaatgccc gtccgccgtc gatagctcgg
      541 tcacgaaacg ccactcgcca cccacacgct tctgaaggat gaccctgcgg tcggatcgct
      601 tgggcaatgc gaccccggag agggtgaccc tgcgtccgga catcacgtgg cggcgcgaag
      661 cctccagcgt gacgcgtgcc tcgacttgga tccgggcgcg gcccctgctg aaggtcggct
      721 cgccgtcggg tgtcttggac ttgagcctgt actccgcggt tcgcgtcaga tcctcgagcc
      781 ggaaagacac gtcgccgtcc tcgtcgacgg atctcgtgag cagtttgatc cagcccccgc
      841 ccttgtactt ctgaagcgtg accttctcgc ctccggtccc gttctccatg tgtcccgtca
      901 cgaccgcggt cgagcgaaac gaaacctgcg ctggagcgga atccagctca ggacgagctt
      961 gttcttgcgc tgctgcgacg gccgggatcg ctagaacgaa tcccaccgct atcaccgccg
     1021 cgcgcgcgag ttgaggcagg cgcatgtcgc gtcgtctcct ttgcttagcg cgatcgccga
     1081 ggcgaccgct gtgcttagcg cgatcgccga ggcgaccgct ggtcgtgcgc ggctagatgc
     1141 cgccgctcaa ccaattccgc ctctcgcctt cgtcttgagg ctccacggcc gctgccaggt
     1201 gggcggccgg aggcgcttac aggaacggca ggctaacgct ggagttagct atgcgcaagc
     1261 agtagttagc tgtgagtaag cgtggagcta cgaagagtgg ttacagcccc taagccccct
     1321 cgcttgcccc tgcgagggtg cctaggcccc ctcgcgtaag ccctcgacgt agcggtggaa
     1381 ggcgaggtga tcggacgtgc attcctcggt ggcgccgtcc ttcgtcagct tgacggtgtc
     1441 gctgggtcct cccggcgcgt tgggatcttc cgtgatctcg accacttccc aaccgtcgtc
     1501 gatcagaccg cgcagcttct cgagttcggg gtcgtctccc catcccgcca tgcacgaacc
     1561 tccatccctc ggtttattag gtttcttaga acaacgccgc tgctaggcta aaccgcccaa
     1621 tgtctgaggc gcgcagcgac ttcaccgatt tccagcaggt cttttcccgg atcgtcgaca
     1681 acatcgagaa gacgatccag gggaagaagg acgtggtccg gctcgctctc gtagcgctgg
     1741 tcgccgaagg acacttgctg ctggaggacg tccccggcgt cgggaagacc atgctcgcca
     1801 agtccctggc gaagtccctg gggtgcagct ggtcccggat ccagttcacc cccgacctgc
     1861 tgccctccga catcaccggt gtcaacgtgt gggatgcccg caccggggag ttccgcttca
     1921 aggcgggacc ggtgttcgcg aatatctgcc tgggcgacga gatcaaccga gcctcgccca
     1981 agacccaggc tgctctcctc gaatgcatgg aagagcgcca ggtcaccgtt gacggagtca
     2041 ctcacgagct ggaagcgccc ttcatggtga tcgcgaccca gaacccgatc gagcatgagg
     2101 gcacctaccc gttgcccgag tcccaactgg accgtttcat catcaggttg tcgatgggtt
     2161 accccgagcg ggagtccgag ctcgagatcc tcgagcgtca cggcgcacac gaagtgctcg
     2221 acgatctcgg agccgtttct gatgccgccg agatcgcggg gctcgtcaag ctggcccgcg
     2281 acgttcattg cgcacccgct ctgaggaact acatcgttca gttgaccgaa tcgacgcgtg
     2341 gccatccgga catctacctg ggtgcgagcc cacgcgcttc gctcttcctg ttgcgtgcct
     2401 cacgcgcgct ggccgccgcg cgcggacggg acttcgtggt gccagatgac gtacaggacc
     2461 tggtggtccc tgttctcgct caccggatcc ttctttcgcc cgaggcacag atgcgtgggg
     2521 gtacctcgga cgaggtgttg atgggtgtgc tcgagaggat cccggtcccc gtccgcactc
     2581 aggcctagtc cgcccatgcc caccagacgc gggctactga tcgcctctgc cggggtcgct
     2641 ctcctcgtca ccgggatcgt cttcggttcc agagccgtcc aacagttggg tttcgcactg
     2701 atcgccttgc tgctcatcgg gatcgccgtg gttcggatca gccggcacga cctcgcggtt
     2761 cacaggcaga tcgccccgca acgagcgcga gctcatcacc ccgtgacgat cacgctctcg
     2821 atcgagaact tcggtcgcgg ccgagcccct ctgatcatgc tcgaggatcg cctgccgccc
     2881 ggactggcag gccgcgcacg cttcctactt ccgtcgctgg aatccggggg caaacgagag
     2941 gctcctataa ccgttcgtcc ggagcagcgc ggtcgctaca aggtcggccc tctcgaggtc
     3001 gaggcggccg acccgttcgg tcttgcccgc gtccgctcgg tcgcattggg aacttcggag
     3061 ttcgtcgttc acccacgcat cgaatctctc gtgatgccga gagactcggg cgaacgaagg
     3121 agcacggcgc gcgcgacgac gcgacacgcg accggtccgc gcggcgaaga cttctacacg
     3181 atccgagagt acgtcgaggg cgacgatctg aggaagatcc actgggcttc cacggcgaag
     3241 cgcgccaagg cgatgatccg ccacgaggaa accccgtggc agacgcgcgc gacgatcgtg
     3301 ctcgacgacc gcaggctccc ttacggcggc ttcagcgaac acatctcttt cgagcgggca
     3361 gtgtcggcgg cagcttcatt ggtcgacctc tacgcgcgtg cgggatacgg ctatcagctg
     3421 atcggcgctc atcatcctgg tatcccctcg ggtaaaggcg aggaccactt caaccgctgt
     3481 ctcgatctgc tcgccacgat cgaaccgaag gggaacccga aacaagagga ccctctcctc
     3541 cttgctcgac tgaccgagct ggaagcgcgc aggggtggag aggccagttt ggtcatcgtc
     3601 accgggtcgt tggatcccac agtcggtgtc gcgatcgcca gattgcggcg cgtctaccac
     3661 cagatcatcc tcgtcgcgtt cccctctcac agattcggaa ccgccacgac gtcggcgcga
     3721 tgggagggag aacgcgccgc gcgcgaggta gccgccctga taactcgagc cggcggacgc
     3781 gttctcgtat tgggacccgg tgatcgtttc gcgcccgcat gggcgtcttt ctcgagccga
     3841 accgcgacaa gtgagatgag atgggatctg aagccagaac acgtctagca ctcgccggcc
     3901 ttctcgtggt cacgctgctg gctttctcgc gcgtattcca ggaaggggat tacccgggac
     3961 cggccttact gggcatgctg cttgcggtcg gcctcgctca gctgacgcgc cgggtgggac
     4021 tcgtcgtctg ggcttcggcg ctggtctcga tgttcgccct cggctggtat ctctgcctga
     4081 tcttcgccgg gaacctcacg ttctacggtc tccccacacc ggctgcgatc gcgaagatcg
     4141 ggacgctcgt ggtcgacgct tacgagaaga gcagcctcga ctacgccccg gttccccttc
     4201 gccccggtta tgtcctgcta acgatcgccg caatgtggct gcttgcagca ctgggagaaa
     4261 cggcgacctt caggtggcgg cgtccgttga tccccgcgat cgcatcggcc gcgatgttct
     4321 cgttcgttct gatcgtcggc atcaggaaag gatcgtcgtt cctcacgatc gtctacctgt
     4381 taggcctgct gacctatctg ggcctcgagt ccgcgcacag gctgggttcg tgggggcggt
     4441 gggtctccgg gtgggcgaag cgatgggact cggaggtcac taccggccct ctcgcccgca
     4501 agatgggcgc ctcgtgtctg gcagccgcgc tgatcgcacc cttcttcctt ccggccctgg
     4561 gcaaggggct gctgtcgtgg cgaagtggga tcggtggcgg ggccggcggt gagatcgaca
     4621 ctctcgtctc gctcgccccg aagctcgtca aacaatcgaa cgacgtcctg ttcgaggtcc
     4681 aggcgccgag gcccgcctac tggagactcg tgaccctcac gtacttcgac ggcaactcgt
     4741 ggcatcccga accgaaccgc gagacgctgg cgaacgggcg ggtggcgagt gatctaccgc
     4801 ccccgaaccc gggcgcgccg ctgctccagc ggtacgagat cagggggctg gagggcgaac
     4861 cattgccggc agtgcctctc cccgagaccg tgaccatgac cggcgacgac gccgatgtcg
     4921 ccatggagtt caatccgatc accgcggacg tcgaggtcgc cgactcggtc gaggagggca
     4981 tgacctatga ggtcagatcg ttggtgccga acgtcacctt ccgatcgatg cgacgagcga
     5041 cgatcccgca ggtcaaggac gtcgccgaga gcctcggtct tcccgactat ctaccgatga
     5101 atacctacga ggtcccccgc gacttctgtc ctccggagat ccccgggaat gagtgtccac
     5161 ttcaacagag ccggatcttc cgcatcgctc aacgatggac ccagggtgcc gatcgcccct
     5221 tcgacaagct cctgctgcta cagaatcgtt tccgaggcca gttccagcat caactgccgg
     5281 gccgctccga ggtcgccgag tcggaggtga agcctctcgc gtcggccgag tacctcacgc
     5341 gcttcctgac ggagacccgg actggttact gtcagcagtt cgcaacagcc tttgcggtac
     5401 tcgctcggat gctcggctat ccggctcgcg tatcggtcgg cttcctcccg ggtgaaaccg
     5461 cgatcgagag tcccgatcag tacatcgttc gggggaacga cgcgcatgca tggccggagg
     5521 tctacttcga agacatcggt tggatcgcgt tcgagccgac tccgcgcggc gccgcgcctc
     5581 ctccggcata cacccaggcg caagccccgg gagccggtgt caacccgccg gcgctcgatc
     5641 ccgggggccg cgcggcgcgt ggccaacaga atccgcgcgg gatacgagac acgctggatg
     5701 cccgtccggg tcgaacggcc gagcgcgagc gccgtgaacg agaggaactg tggcacgact
     5761 ctttcgtgcg gctcgcgacg actctgggcg tcgtgatcgc gttgctgttg atcgtcaccc
     5821 ctctggcgaa actgtttctg atccgccggc gctatcgcaa ggctcgagac ggagatgcac
     5881 tcgccggtgc ggcattcgcc gagttcgaac aggaagcgag tgatctcgcc tacccacgcg
     5941 ggcacgccga gaccgcgggt gcctatgtga agcgactggt cagatcgcac aacgtgtcgc
     6001 ccgcccgggc ctcgcgactg gccgaactgt tcgaggaggc gatctacgga ccggcggggt
     6061 tgagcgatcg ccagggatcc gaggccgtcc gtctgaccca tgccctgaag aaggacctgt
     6121 gggccgaggc ctcctggacg ctacgggccc agcgcctgtt ctctccgggg gtggtggtcg
     6181 ccgaactcca ggctaacgag cggccctcaa acggcgacgg gtctcgaccg ggaggtcttg
     6241 ctcgagttct gactcgatcc gcctcagagc ggcgatgaac tcctctcgat cgtagagata
     6301 ctcggccact ttcccgtcga tgctccgggc gatgtcgtcg atcgccagga agaacgccat
     6361 ctggccttcc ctcaaggcgt cgaggatctc gccgtcgttc cgagcgagct tgaagatgct
     6421 tctcccgtcg gtcacgagtt tcacctcgga gaggtgctcg tccagagcgg ccctcttgcg
     6481 caggtactcg atcgcgcgcc gaacgcgctg gagactcatc ccgccgtcga gaagtgaacg
     6541 gatcactttc aatgcaacga gatcgcggaa cgaatagaga cgacgaacgc cgggacgacc
     6601 accggtggcc tgcaccgaag gcttcaccaa actgatccgg tcccagtaac gaagttggtg
     6661 tgctgtgcaa cctgtgaaac gaatcgcttg atctgcggtg aaaccttcca tcagagcgcg
     6721 cgaacctccc gtcggtgatt catctactag atcccgcccg ctgggacttc cccccggaac
     6781 gcgaggagcg aatctagcgc gcgcgctcgt aacgcggcaa gccagaatgg cctttcacaa
     6841 gatcttttta tccagcgacc aaaagaagcc gctaccgtcg gcgataacgg tcacggagtt
     6901 tgtcttccaa accaccgaga aatctctgcc cgaaagacag gtcgcgaaat cccgaaccga
     6961 gtcggtcgcg gagagctccc gcaaccaaga cgatgccggc g
//
LOCUS       DATMED010000427         3400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_727598, whole genome shotgun sequence.
ACCESSION   DATMED010000427 DATMED010000000
VERSION     DATMED010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727598"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VNP73_11225"
     CDS             <1..394
                     /locus_tag="VNP73_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66535.1"
                     /translation="SITWTNDGDAPHTVTADNGSFDSGNMDSGDDFSESFTDQGRYPY
                     YCEYHGGPGGEGMAGVVVVTGDDSGGGNGDDTDDPTDPAETTEPATEEGDLPTTGTEV
                     DAFIYLALAMIFAGVVLVKLGSPLTRRR"
     gene            complement(450..950)
                     /locus_tag="VNP73_11230"
     CDS             complement(450..950)
                     /locus_tag="VNP73_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWL66536.1"
                     /translation="MAQEKDIYSPQNKEKREEIISLLTQAYWMEIETVMSYMANSINP
                     DGLRAQEIIESLEADIQEELGHARQFGERIKELYGVVPGSLDFTPEQKYLQPPEEQND
                     IIHVIKGVVEAESGAIEHYNKIISVCDGIDWVTQDMVITILHDEQGHRRLFEGFLREF
                     EASGPA"
     gene            complement(1039..1704)
                     /locus_tag="VNP73_11235"
     CDS             complement(1039..1704)
                     /locus_tag="VNP73_11235"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWL66537.1"
                     /translation="MHDLDKLVLRLDNATADRRLLDHSFYKAWAAGDLTLEDLAHYST
                     QYWRQVEAFPGYLRTIADRVETPEARDILIANLKDEVGQDHPGLWLDFASSVGVKRDD
                     VTEASVEPETTECVSEFGEVAREAPLPYALGMLYGYESQTPAVAETKVAGLREHYEID
                     GPGVEYFRLHGVLDVEHSREMATAISLVSRDDVAEAEAGAARGAGAIWTLLNGVERVR
                     QAT"
     gene            complement(1799..2761)
                     /locus_tag="VNP73_11240"
     CDS             complement(1799..2761)
                     /locus_tag="VNP73_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HWL66538.1"
                     /translation="MVMPKTATKFRLAARGLAGEPVDLPRTFLSHGMADLPPMWVDED
                     TPAFGATISLSKGRPRSVRVTDDSKDRAVVEVFGRAPGSAAKAEIKSTVRHILRLDED
                     LSEFYAQAAEDPDLKWVTSGAGRMIRSATVFEEVVKTVCTTNCSWAMTIKMVSAMVEH
                     LGEPAVGAPAGSHQGRAFPTPEAMASVDESFYREVVRAGYRGPYFRELARRVAESEID
                     LEAVGNAPADEVSDEELDAALQALPGVGPYASATIMMMLGRYSRLILDSWTRPKYARL
                     VGRKSVKDATIARRFRPYGRWAGLAFWLFLTKDWIESEELPPMA"
     gene            complement(2796..>3400)
                     /locus_tag="VNP73_11245"
     CDS             complement(2796..>3400)
                     /locus_tag="VNP73_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66539.1"
                     /translation="LGPSGAASPGATGEGLCHLVVLGLQDSGPSAIAADWLLAARTPA
                     QAWLDSPDDVPGELDESAGSRVWATAAASCGLLAVANDPGPRAIGLLRGEADVDGRFT
                     GGAYPTFAAAGAYWLAEGPKTEMAEWALKWAREWEEDWWGPWERATALTFWGAARIAP
                     DHATVETFLDDLRSAAPAAGWADDLGLTIRLLELLAHFEG"
BASE COUNT          616 a         1116 c         1048 g          620 t
ORIGIN      
        1 ctccatcacg tggacgaacg acggtgacgc cccccacacg gtcacggccg acaacggctc
       61 gttcgactcg gggaacatgg attccgggga cgacttctcc gaaagcttca ccgaccaggg
      121 acgctatccc tactattgcg agtaccacgg cgggcccggc ggcgaaggca tggccggagt
      181 ggtggtggtg acgggtgacg actctggtgg tggaaatggt gatgacaccg acgaccccac
      241 ggacccggcc gagaccaccg agcccgcgac cgaggagggc gatctcccca ccacggggac
      301 cgaagtggac gcattcatct atctggcgct ggccatgatc tttgccgggg tggtgctggt
      361 caagctgggc tcgccgctaa ctcgccgccg ctagcccggc gcgcaaaagg gcgcccttcc
      421 ggacgccctt tggtcagtta tcaatacttt caagcggggc cggatgcctc gaactctctc
      481 aagaagccct cgaagagcct gcggtggcct tgctcgtcgt gaagaatcgt aatcaccatg
      541 tcctgcgtta cccagtcgat cccatcgcag accgagatga tcttgttgta gtgctcgatc
      601 gcgccgctct cagcctcgac gacacctttg atcacgtgga tgatgtcgtt ctgctcctcg
      661 ggaggctgta ggtacttctg ctcgggagtg aaatcgaggg agcccggaac gactccgtaa
      721 agctccttga tccgctcacc gaactgccga gcgtgcccaa gctcttcctg gatgtccgcc
      781 tcgaggctct cgatgatctc ctgagcccga aggccgtcgg ggttgatcga attggccatg
      841 tacgacatga cggtttcgat ctccatccag taggcctgag taagcagcga gatgatctcc
      901 tcgcgtttct ccttgttctg cggggagtag atgtcctttt cctgggccac ggggactcct
      961 ttcgtaccgt cggtacgtct caattgttga acggtctcta gctattgatt gttaccgcga
     1021 agccgcccgt gaaacagctt aggttgcttg tctcactctt tcaacgccgt tgaggagggt
     1081 ccagattgca ccggcacctc gcgcggcccc cgcctcagcc tcggctacgt cgtcccggct
     1141 caccaacgag atcgccgtgg ccatctcgcg cgagtgctcc acgtccagaa ctccatggag
     1201 gcggaagtat tcgacaccgg gaccgtctat ctcgtagtgc tcgcggagcc ccgctacttt
     1261 cgtctcggca accgcgggag tctgcgactc gtagccgtaa agcatgccca gcgcgtatgg
     1321 gagcggggct tcccgagcga cttccccgaa ctccgagaca cattcggtgg tctcgggctc
     1381 gacggaggcc tccgtcacgt cgtcgcgctt caccccaacg ctgctcgcaa agtcgagcca
     1441 caagcccgga tggtcctgtc cgacctcgtc cttcaggttg gcaataagga tgtcacgagc
     1501 ctccggcgtt tcgacccgat cggcgatagt gcgcaggtat ccggggaagg cctcgacctg
     1561 ccgccagtac tgcgtggagt aatgggcgag gtcctcgagc gtcaggtcgc cggccgccca
     1621 ggccttgtag aacgagtgat ctagaagacg ccgatccgca gtggcgttgt cgagcctgag
     1681 gacgagcttg tcgaggtcgt gcatcaaaag cctcccgcta cttgatctgc taaccgcccg
     1741 gacattacta gcttctcgat ccggaggggg tcacccgggg gatggcggtg gccaaacatc
     1801 acgccatcgg tgggagctcc tcggattcga tccagtcctt cgtgagaaag agccagaatg
     1861 cgaggccggc ccagcggccg tagggacgga accgtctggc tatcgttgcg tccttcaccg
     1921 acttgcgtcc taccaggcgc gcgtacttgg gccgcgtcca cgaatcaagg atcaacctgg
     1981 agtatcggcc gagcatcatc atgatggtcg ccgaagcgta cgggcccacc cccggcaaag
     2041 cctgcagtgc cgcgtcgagc tcttcgtcgg aaacctcatc tgcaggcgcg ttgccgacgg
     2101 cttcgagatc gatctcggat tcggcgacgc gtcgagcaag ctctcggaag tacggacccc
     2161 ggtacccggc ccgaacgacc tcccgataaa acgattcgtc cacggaagcc attgcctcgg
     2221 gtgtgggaaa ggctctgccc tgatggctgc cggccggcgc tcccacggcc ggctcgccga
     2281 ggtgctcgac cattgccgac accatcttga tcgtcatcgc ccacgaacaa ttggtcgtgc
     2341 agaccgtctt gacgacctct tcgaagaccg tcgcgctccg aatcatccgt cccgccccgg
     2401 aggtgaccca cttcagatcg ggatcctcgg cggcctgggc atagaactcg gagaggtctt
     2461 cgtcgagacg aaggatgtgg cgcaccgtgg acttgatctc agccttcgcg gccgacccgg
     2521 gcgcgcggcc gaacacctca acaactgcac gatctttcga gtcgtcggtt acgcgtacgg
     2581 agcgcggtcg tcccttcgac agcgagatcg tggctccgaa agcgggggtg tcttcatcga
     2641 cccacatcgg agggagatcc gccatcccgt gggacaggaa agtccgcggc agatccaccg
     2701 gttctccggc aagacctcgg gcggcgagtc ggaacttggt agcggttttt ggcataacca
     2761 tcaaaagggt tttgctagtt acggcagcct agcactcagc cctcgaaatg ggcgagtagc
     2821 tcgagcagac ggatcgtcag gcccagatcg tccgcccatc cggcggccgg cgcggcggaa
     2881 cgcaggtcat cgaggaaggt ctcgaccgtt gcatgatcgg gcgcgatcct cgcggcgccc
     2941 cagaacgtga gcgccgttgc acgttcccac ggcccccacc aatcctcctc ccactcgcgc
     3001 gcccacttga gggcccactc ggccatctcg gtcttcgggc cctccgccag ccagtaggcg
     3061 ccggcggctg caaacgtcgg ataagcaccg ccggtgaacc gcccgtcgac gtcggcctcg
     3121 ccgcgcagaa ggccgatggc gcgagggccg ggatcgttgg ccacggctaa caagccgcag
     3181 gacgccgccg cggtagccca gacgcgcgaa cccgcggatt cgtcgagctc gcccggaacg
     3241 tcgtcgggag aatcgagcca tgcctgcgcc ggggttcgcg ccgcgagaag ccagtccgcg
     3301 gcgattgccg agggtccgga gtcctggagc ccaagcacga ccaggtgaca aagaccttcg
     3361 cccgtcgccc ccggcgaagc ggcgcccgac ggtcctagaa
//
LOCUS       DATMED010000428         2893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4566181, whole genome shotgun sequence.
ACCESSION   DATMED010000428 DATMED010000000
VERSION     DATMED010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4566181"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VNP73_11250"
     CDS             <1..460
                     /locus_tag="VNP73_11250"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HWL66540.1"
                     /translation="GAIRPGYTPIHDAISRLAELGAPNRYIVTTGMIVFGLGCILFAP
                     RLRKPAVISLTVSGLASFGVALFPCTQGCPGMGSVTDTAHSMFAGLHYVGFALTPVLH
                     ARRPGAIALVAFAGAALLIHATGVGPNGLFQRVGLTTLDAWLVGTAVRLR"
     gene            493..1203
                     /locus_tag="VNP73_11255"
     CDS             493..1203
                     /locus_tag="VNP73_11255"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HWL66541.1"
                     /translation="MQIVQKLAAGRTAHPYRDIDVIDVRAPRFNQGVVALMCVLALIT
                     GSWWFASVMGLQLAIGLLFGRQFCVTCLFYFEVLQPRFGEGPIEDARPPRFANILGAI
                     FLLSATTLHLAGLATAGWVLIGVVAALATLAVTTGFCMGCTMYRVIARFRGVRPGHHD
                     HIDMRDFDVTSDAVIQFTHPLCSDCKEMERELRAKEGDLVVVDVSKQKDLARKYHVDV
                     VPLALRVSSEGRVLQRLA"
     gene            1163..2149
                     /locus_tag="VNP73_11260"
     CDS             1163..2149
                     /locus_tag="VNP73_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HWL66542.1"
                     /translation="MFRAKAEFFNDSLKLRALTMEHTETETKLAEEIVDAMGKVHGTH
                     TGFRAVHAKGLCCKGHFVATPEAANICVAPHLQGDEIPVTVRFSSGSGKPTRADGAKD
                     ERGMGVKFHLPDGKATDIVSLTLPVFFVRTPEDFLEFLKAQEADPDTGKPDFDRVGSF
                     IDEHPETKQAVTFVMFSESPASFANCTFFGIHAFRMRGPGGGERWVRYRWLPEAGSAT
                     LTDEKTRELGKDYLRAELEQRITSESIIFDLQFQLAEEDDDPNDPTTQWPEERTFVPA
                     GRLTLTEYVDQECAPMVFDPGNLAEGIERSDDPILHMRSPAYSVSYERRSQT"
     gene            2158..>2893
                     /locus_tag="VNP73_11265"
     CDS             2158..>2893
                     /locus_tag="VNP73_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWL66543.1"
                     /translation="MRRTKIVATLGPASHEPGVIDALIEEGADVLRLNFAHGDPTFHK
                     ETVARVRAAAERNERVVGILGDLPGPKMRTGPIAGGEVLLEAGQSFLLRSGYDTGDET
                     ACCTTVTGLDRLVATGEEIFLADGQIVLEVGAIESGTVHTQVVRGGVLRSGKGLHIPG
                     AERKVDAFTARDEEALSTAVELGVDFIGLSFVRGKEDLERVREKLQSYEQVPALLAKI
                     ETRAAVENLDEIVSSADAVMVARGDLG"
BASE COUNT          516 a          896 c          935 g          546 t
ORIGIN      
        1 cggggcgatc cggccgggat acacaccgat ccacgacgcg atcagtcgtc tggccgagct
       61 gggtgcaccg aaccggtaca tcgtcacaac ggggatgatc gtgttcggcc tcgggtgcat
      121 cctgtttgcc ccacgacttc gcaagcccgc cgtcatctct ctgaccgtgt ccggactggc
      181 gtcgttcggc gtcgctctct ttccctgcac ccagggatgc ccggggatgg gttccgtcac
      241 cgataccgcc cactcgatgt tcgccggctt gcattacgtg ggcttcgctc tcaccccggt
      301 gctgcatgcg cgccggccgg gagccattgc actcgtggcc ttcgccggcg ccgcccttct
      361 gatccacgcg accggcgtgg gaccgaacgg gctcttccag cgcgtagggc taacgacgct
      421 ggatgcgtgg ctggtaggga cggcggttcg cctccgttag tcggtctgcc ccccgattgg
      481 tactatttca ctgtgcaaat agttcaaaag ctggcggcgg gccgaacggc ccacccatat
      541 cgggacatag acgtcatcga cgtacgggcg ccgcgcttca accagggcgt cgtagcgctg
      601 atgtgcgtgc tcgctctgat cacggggtcg tggtggttcg cgtcggtcat gggccttcaa
      661 ctggcgatcg ggctgctgtt cgggaggcag ttctgcgtga cgtgtctctt ctatttcgag
      721 gtactccaac cgcgcttcgg tgaaggcccg atcgaggacg cgcggccccc tcgattcgcc
      781 aacatcctcg gcgcgatctt ccttctgtcc gcgaccacgc tccacctcgc cgggctggca
      841 acggccggat gggtgttgat cggtgtcgtg gccgcgctcg ccacgctggc ggtgaccacc
      901 ggcttttgca tgggctgcac catgtatcgg gtgatcgccc gtttccgtgg tgtcaggccc
      961 ggtcatcacg atcacatcga catgagggac ttcgacgtga cctccgatgc ggtgatccaa
     1021 ttcacccatc cgctctgtag cgattgcaag gagatggaac gagagctgcg agccaaagag
     1081 ggcgacctcg tggtggtcga cgtctcgaag cagaaggatc tcgctcgcaa gtaccacgtc
     1141 gacgtagtcc cactcgcgct gcgtgtttcg agcgaaggcc gagttcttca acgactcgct
     1201 taagctccgt gccctgacca tggagcacac cgagaccgag acgaagctcg ccgaagagat
     1261 cgtcgacgcc atgggaaagg tccacgggac ccacaccggc ttccgagccg ttcacgccaa
     1321 gggactgtgc tgcaagggcc acttcgtggc gacccccgaa gcagcgaaca tctgcgtggc
     1381 gccgcatctt caaggcgacg agatccccgt caccgtccgt ttctcgagtg ggtcgggcaa
     1441 gccgacccgc gccgacggag ccaaggacga acgtgggatg ggggtgaagt tccatctccc
     1501 agacggcaaa gccaccgaca tcgtctctct taccttgccg gttttcttcg tgaggacgcc
     1561 ggaagatttc cttgagttct tgaaagcgca agaggcagat ccggacaccg ggaaacccga
     1621 tttcgatcgt gtcggctcgt tcatcgacga acatcccgag acgaagcaag ccgtcacgtt
     1681 cgtgatgttt tccgaatcgc cggccagctt cgccaactgc acgttcttcg gcatccacgc
     1741 gttccgcatg agggggccgg gcggagggga gagatgggtc cgatatcgct ggctccccga
     1801 ggccggttcc gcgacgttga cggacgagaa gacgcgcgaa ctcggaaagg actacttgag
     1861 ggcggagctc gaacaacgca tcacgtctga atccatcatc ttcgacctgc agttccagct
     1921 ggctgaggaa gacgacgatc ccaacgaccc cacgactcaa tggcccgagg agcgcacgtt
     1981 cgtgcccgcg ggccgtctca cattgaccga gtacgtcgac caggagtgcg ctccgatggt
     2041 cttcgatcct gggaaccttg ccgaagggat cgaacgttcc gacgatccga tcctgcatat
     2101 gcgaagtccg gcctattcgg tttcgtacga gcggagatcc caaacctgag caccgggatg
     2161 cgcaggacga agatcgttgc caccttgggc ccggcctcgc acgagcccgg cgtgatcgat
     2221 gcactcatcg aagagggtgc cgatgtcctg cgtctgaatt tcgcccacgg agatccgacc
     2281 ttccacaagg agaccgttgc gagggttcgg gccgcggccg agcgcaacga acgcgtcgtg
     2341 gggatcctgg gcgacctgcc cggaccaaag atgcgaacgg gtccgatagc cgggggggag
     2401 gtgctgctcg aggccggtca atcattcttg ctgcggtccg gctacgacac cggcgacgag
     2461 acggcgtgct gcacgactgt gaccgggttg gatcggttgg tggcaacggg cgaggagatc
     2521 ttcctggcgg acggccagat cgttctcgag gttggcgcga tcgaatctgg aacggttcac
     2581 acgcaagtag ttcgcggcgg cgtgctgcga tccggcaagg gcttgcacat cccgggggcc
     2641 gaacgcaagg tggatgcgtt caccgcccgc gacgaggagg cattgtcgac cgcggtcgag
     2701 cttggtgtgg acttcatcgg gctctcgttc gttcgaggta aggaagatct cgagcgagtg
     2761 agggagaagc ttcagagcta cgagcaggtt cctgctctgc tcgcgaagat cgagacccgc
     2821 gccgccgtcg agaacctgga cgagatcgtg tcgtccgccg acgccgtgat ggtggcgagg
     2881 ggcgatctcg gca
//
LOCUS       DATMED010000429         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_358360, whole genome shotgun sequence.
ACCESSION   DATMED010000429 DATMED010000000
VERSION     DATMED010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358360"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1264)
                     /gene="dnaN"
                     /locus_tag="VNP73_11270"
     CDS             complement(131..1264)
                     /gene="dnaN"
                     /locus_tag="VNP73_11270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWL66544.1"
                     /translation="MKFRCERDDVLGAVQFASRAISTRATLPVLAGLRIEALEGGQVR
                     VAATDLELTMETSFQAAVDEPGRTIVPGRLFGEMTRSLTAGQVSVAGNESEVELGSGR
                     GQFRVKALAPDDYPSLPIEDQSGGGDSIDSDIDAAALATALSQVVRSASSDESRQILT
                     GVLWELSKDSLTLAATDSYRLSVRELAVAGGPDDTRKVVLPARALAELNRALAGTTGV
                     RATVKENLVSFSISGSSEPGLQDAIIGSRFIEGEFPKYRQLLPDGYPNNLTVSKDGLL
                     DVTKRVGLLAQNNLPLKLRLGDELEVSAHTPDVGEGQEVLDATYEGEPLVIAFNPQFL
                     YDGVSAVQGEEVVMEIGDGLKPAIIKDPNDAGFTYLLMPVRLS"
     gene            complement(1540..>2299)
                     /locus_tag="VNP73_11275"
     CDS             complement(1540..>2299)
                     /locus_tag="VNP73_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWL66545.1"
                     /translation="AFHRRYRVADLLLVDDIQFLEGKERTQEEFFHTFNALHPKSQII
                     ITSDRPPKKIPTLEERLRTRFEWGLITDIQPPDLETRLAILQKKSETERLSIPREVMS
                     YIAEKIQTNIRELEGAVIRVAAYASLTRSDVTLELAQGVLQSLLPNTSEARVTSDLIV
                     SVTAEYFDVTHEEICSASRSRPLVNARQMAMYLCRELTDLSLPKIGERFGGRDHSTVV
                     FAVNKVKNQMKERETSYEQVRELTTRIRQQAGRG"
BASE COUNT          418 a          705 c          718 g          458 t
ORIGIN      
        1 gcggcccacg atcaggttca gccccggaac gaaatcgatc cgttcgcgtt cgtagttcct
       61 gaagtcctcc agctggatgt agtcgacgtg caccgaagga cctccatctt cgcttcgacc
      121 ggcgcttccg ttacgagagc cgcaccggca tgagcagata ggtgaagccg gcgtcgttcg
      181 ggtctttgat gatcgcaggc ttaagaccgt caccgatctc cataacgacc tcttcacctt
      241 gcaccgccga caccccgtcg taaaggaatt gggggttgaa cgcgatcact aggggctcac
      301 cttcgtaggt ggcgtccagc acttcctgcc cctccccgac gtccggggta tgagcagaca
      361 cctcgagctc gtcgcccagc ctgagcttca aggggaggtt gttctgcgcg agcaggccga
      421 cgcgcttggt cacgtcgagc aagccgtcct tggataccgt gaggttgttc ggatagccgt
      481 ccggcaggag ttgccggtac ttcggaaact cgccttcgat gaaacgcgaa ccgatgatcg
      541 cgtcctgcag ccccggctcg gaactgccgg agatgctgaa cgaaacgagg ttctctttca
      601 ccgtcgccct cacaccggtc gtgccggcga gagcccggtt caattcggcg agagcccgag
      661 caggaaggac gaccttccgg gtgtcatccg gaccaccggc gaccgccaac tctcggacgc
      721 tcaggcgata ggagtcggtg gccgccagcg ttagcgagtc cttgctgagc tcccacagga
      781 cgccggtgag tatctggcgg ctctcgtccg aggacgcgct gcgaacaact tgggagaggg
      841 ccgtcgctaa tgcggccgca tcgatgtcgc tgtcgatcga atcgcccccg ccgctctgat
      901 cctcgatcgg aagcgagggg tagtcgtcgg gcgcaagtgc cttcacgcgg aactgccccc
      961 gacccgaccc gagctcgacc tcggactcgt tgcccgcaac gctcacctgc ccggcggtaa
     1021 ggctgcgtgt catctcgccg aacagccggc cgggaacgat cgtgcgtccc ggctcgtcga
     1081 ccgcagcttg gaacgacgtc tccatcgtca actcgagatc cgtggcagcg accctgacct
     1141 ggcctccctc caacgcctcg atccggaggc ctgcgagcac cggcaacgta gcgcgggtcg
     1201 atatcgcccg cgacgcaaac tggacagcgc ccagaacgtc gtctcgttcg caacggaatt
     1261 tcatctagcg acctccctcg aactttgtaa cgacattaaa gaacaagcgg cgatacggaa
     1321 gtgatctagt ccacagttct gatcttcgag agataaaggg ttttctctcg tagtactact
     1381 aggggctgtt ggtatgcgga taactgcatg gatgccctgg tcgcgcgggg tcgcgtgctg
     1441 ttgggcgtac cgggacgaac gggtgacgat ccatcgcgct ctgtggaggc gaggcgaagc
     1501 cggcccggtt gtccccagtc acccacaggt gtcatctcat cacccacggc cggcctgttg
     1561 gcggatcctg gtcgtgagct ctcttacctg ctcatagctg gtttcgcgct ccttcatctg
     1621 gttcttgacc ttgttgacgg cgaagacgac ggtggagtgg tcgcggcccc cgaagcgctc
     1681 tccgatcttg ggaagagaga ggtccgttag ctccctgcag aggtacatcg ccatctgccg
     1741 cgcattaacg aggggccgcg agcgactggc tgagcagatc tcctcgtgcg tgacgtcgaa
     1801 gtactcggcg gtcacggaaa cgatcaggtc ggacgtcacc cgcgcctcgc tcgtgttggg
     1861 gagtagggac tgcagaacgc cctgagccag ctccagagtt acatctgagc gcgtcagcga
     1921 cgcgtaagcc gcgacgcgga tcaccgcacc ttcgagctcg cggatattcg tctggatctt
     1981 ctcggcgatg taggacatga cttcgcgcgg gatggagaga cgctcggtct ccgacttctt
     2041 ctggaggatc gcaagtcgtg tctcgagatc gggtggctgg atgtcggtga tcaagcccca
     2101 ttcgaatcgc gtccggagcc gttcctcgag tgtggggatc ttcttgggcg gacgatcgga
     2161 agtgatgatg atctgactct tcgggtgcag ggcgttgaac gtatggaaaa actcttcctg
     2221 cgtgcgttct ttgccctcga ggaattggat gtcgtcgacg aggagcaggt ccgcgacccg
     2281 gtatcgacgg tggaaggca
//
LOCUS       DATMED010000430         8914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5336645, whole genome shotgun sequence.
ACCESSION   DATMED010000430 DATMED010000000
VERSION     DATMED010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5336645"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..3133
                     /locus_tag="VNP73_11280"
     CDS             83..3133
                     /locus_tag="VNP73_11280"
                     /inference="COORDINATES: protein motif:HMM:NF039970.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWL66546.1"
                     /translation="MEFVVDGVVVGEAPARIIWDTRGVPDGPHWIRARAKVGSQTFSS
                     ERVKVFVRNRLTPSNRVRSEWKARRLSVDDYLRWGVLSLTDSRLLPDRFQSKHDAEGD
                     QTDALYEFLSVWKKARAETKREVREVLARPAVGVQPQPRSHGVVAASSLFDGEVTTQH
                     FQITYDPDSYSAADDLETWNPYANGSAGRASQSNDEPDDIDRLAFAFERAWGVYANVL
                     DFDTPVSPIEIAVTTPVCGEGCGGFTNPFDDHNIQVNPNSANIEPFYIARHELFHAFQ
                     FQYVSGAEHLANFDPNTWWLEGTAEWAAHQAASPISDPEEARDDNDNYARHLPVFFDA
                     PEGRLTTSSLYSIGRPYGTFIFSEFLEERFDVSIVRRVWEEMSGPSNPPADEAVDTVI
                     EFENDSLADVTADFWRANYRLEVGYQDSDRVDVWQSYLGSSERPARVPTNMEVGQSAS
                     GTAEIFAGGSVFVDFSPPASAGPTTLTVETTAADGRGQEDLEVSFLPFDDYPGLCSAG
                     ISSGTSFEIDLGEDCEFATLVLTHTNPRAAVTDSREVNWTATLQEVPGPYDEEANDDS
                     PAGYWRLGEPDPNPAIDETGNPSGSYRGTKQGVAGALGGSNTAITTNVSTDHVVVGGR
                     NNLNNFSANKLTLEAWVKPEWSKMGSNAAIISKPWRDGSWNSPWGQYGLVRLPGTNRL
                     VFEFRDPSEGYHSVTSASSLSADTWHHVAGTFDGDTLRIYINGELDHSSTWTGSIAQR
                     SSDLAIGTRSDSSPGESWVGGIDEVAIYASALAGGRVDAHHDASAANYRGEVMADSPV
                     GYWRLGEPTPNQSIDETGNGNGIYKNVAQGVSGALDDSNAAITTRGGASHVVIPGRGN
                     INNLTDELTLEAWVKPEWSKMGTNAAIISKPWRDGSWNSPWGQYGLVRLPGSNRLVFE
                     FRDPSEGYHSVTSATSLSADTWYHVAGTFDGDTLRIYINGELDHSSTWTGSIAQRSSD
                     LAIGTRSDSSPGESWVGGIDEVAVYDHAVSAERVSMHFRAASTD"
     gene            complement(3494..4513)
                     /locus_tag="VNP73_11285"
     CDS             complement(3494..4513)
                     /locus_tag="VNP73_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66547.1"
                     /translation="MATRAEWFDEEVGPSFEREWPEIARRLERFLAAKGVERWLRADV
                     IQETAARLYPRWKTLDHSQPLWNLAATIAMRVVYNHRRKESRIELVPDPVPMHNDDVH
                     VRGLQRAQLDKTRSALQQLNADQRQVLLAEVGEALLPAGTRNRVKVLRLRARARLREE
                     LGPWAPSAIAIKLRYLAIRAARKESALEMHAPTMANSVANVVMAATLGLAGAAVGGKI
                     PAVGPENPLQDERARLPTVAELRDVGLDPTASIRRRPPGHSRTKPGADRTYGSAPGFV
                     DDTNTWAEEQKKEIDEQKKEIEDQKKEVDEQKKEVENQKKAAEDWAEGSKEEVEEEVE
                     DQLER"
     gene            4728..5078
                     /locus_tag="VNP73_11290"
     CDS             4728..5078
                     /locus_tag="VNP73_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWL66548.1"
                     /translation="MSRNAPSTEEVPGWVILSPTAVPDAWRSRTIPMVLVPLTPAEAS
                     ELLSDATVEQGIAPADLPLIHLVARGYSAAEISRELGVALRTVNRHVARLRDEFGVAT
                     IQELASELARRGFG"
     gene            5177..5887
                     /locus_tag="VNP73_11295"
     CDS             5177..5887
                     /locus_tag="VNP73_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66549.1"
                     /translation="MMKHSVGWMRAASRRAVMTALAVSVVGSGLAAAPAAGESPTKVP
                     KVVQVKDPAGDANFVNNQTEPGEERNDVTPADLSVPDILSVWFTHDVKTISVHIQTEA
                     PPPGSESAYRFAVTVNPENPELCLYLEAFIPGPTWSGGDYSTRAMPCWPDGVVPDEGR
                     VPGELEIHESADGTGITSMTFPRWAGRAFADGEALESPSARTQVVTGAKNGGLEGTFL
                     TAYVDRTSFGKTYVVTNR"
     gene            5944..7134
                     /locus_tag="VNP73_11300"
     CDS             5944..7134
                     /locus_tag="VNP73_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWL66550.1"
                     /translation="MEPVEAAQGSLEKRPFTVVAAVDTGINPYHVDFRTPELTQHPST
                     YITGFPRSAEALNLTLSAETYEEAIARDRAKWDSVDPNKLYWVPGTNIVGAFGRMAAA
                     PNFLDQDGHGTAVASAAGGRVYGPRSQDILIAAVAGHPNNLEWAARQPWIDAITNSYV
                     GCCPPVPQHSRMSRAAVESGKLVCFATGNFVAPVLTAGTQGPSWILSVGAASQKTRGE
                     HVYTSAPADILGLSGVEIAQYQSIDGGEGGYNFGTSMAAPAACGQAARTISEVRRVLG
                     DYTEGAADGLASGRKLKGGYLKDGCLDRVELEDSLQATAVPAETSAPDPDDPLANPAP
                     PVGGFVRGGYGIVDHDSANTAIGVLLGRAPRPERALEDAWIMHTDSIRDALYGPPPEP
                     IGCP"
     gene            complement(7195..>8914)
                     /locus_tag="VNP73_11305"
     CDS             complement(7195..>8914)
                     /locus_tag="VNP73_11305"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HWL66551.1"
                     /translation="NTGSMSGDPAIPFIAEAWCRGLVDEPMADELYDAMWSLASRRSD
                     DYLRLGYLPVPQPSDPADLVDGGPRETGTTLEFGVAEFALALIADASGRTSDHDVLLS
                     RSTGYRHLLDPETRWIRPRDASGAWAEPFAPELGYGFQEGTSWQYSWLAMHDLRGLFD
                     RMGGDDEVSLRLDEFFSFPGAAAAPVVVPKAQNQATLFGIAYYGNQYAPGNEHDLQAP
                     FLYNYAGAPWKTQAVARAAASLYTPTTDGLPGNDDLGALSGWLVWTMLGIYPMTPGAP
                     LYATASPVFERAVVHRPGRSDLVIEAPGTSPVAKYVASATLGGSPLDRAWLTEDELDG
                     ATLRLQMSEVPSMEWGAGPDAAPPSLSTHATSSFGCRPSSPSLEPTDVSFIESSHVSG
                     QFSDKTVFEARLTDSNGHPIAGQELSFELTGTESPRSFSATTNDDGVGAFMPTLEEKP
                     GFYQLTVRFGGDDAHEGDADTTPFVVEKEDTDLTLTVEGDDEDRRVRARLSDLDTPSA
                     SIVGRTIKLYSDSELIGSSKTDGDGVATFALPPGHRGANRNYKVVFEGDDYYLGSSAQ
                     RGGKGG"
BASE COUNT         1684 a         2713 c         2784 g         1733 t
ORIGIN      
        1 tctgaatgtc tcctttggcc aagtgttatt ccacgaagag atgctggtgg caactcttcg
       61 tactccggcg gatacggaga acatggaatt cgtcgttgac ggtgtagtcg taggtgaggc
      121 gcccgcccga atcatttggg acacaagagg tgttcccgac ggacctcatt ggattcgagc
      181 gagggccaaa gtaggcagtc aaacgttctc ctctgaacga gtcaaggtat tcgttcgcaa
      241 tcggctaact ccgagcaatc gggtacgaag cgaatggaaa gcgcgcagat tatcggtcga
      301 cgattatcta cgctggggcg tgttgtcgct gacagattct cgtttgctcc cggaccgatt
      361 tcaatcgaag catgacgcgg aaggagacca gactgacgcg ctctacgagt ttctgagtgt
      421 ctggaagaag gcaagagcag agaccaaaag agaggtcaga gaagtcctcg cgcgtccagc
      481 ggtcggcgtg cagcctcagc cacgctcaca cggagtcgtc gcagcttctt ctttgttcga
      541 cggagaggta accacccagc atttccagat cacttatgac ccagactcat actcggccgc
      601 tgacgatttg gaaacctgga atccatatgc gaatggttcc gccggtaggg ccagtcagag
      661 taacgacgaa cctgacgaca tcgaccgcct agctttcgca ttcgagcgtg cctggggggt
      721 ctacgccaac gtgcttgatt tcgacacgcc ggtgtccccg attgagattg cagtgacgac
      781 cccggtctgc ggggagggat gcggggggtt caccaatccg ttcgacgacc acaacatcca
      841 ggtcaatccg aattcggcca atattgagcc gttctacatc gctcgtcatg agctgttcca
      901 cgcgttccaa ttccagtacg tctccggggc tgaacacttg gcgaatttcg atccgaatac
      961 ttggtggctg gaaggaaccg cggaatgggc tgcccatcag gcagcatcac cgataagcga
     1021 cccagaggaa gctagagacg acaatgacaa ctacgctcgc catctgccgg tatttttcga
     1081 tgctcccgaa ggtcgcttga cgacctcctc gttgtactca attggtcgac cctatgggac
     1141 gttcatcttt tcagagttcc tcgaggaacg atttgacgtt tcgatagtca ggcgcgtctg
     1201 ggaggagatg tccggcccct ccaatccgcc cgcagatgag gcagttgaca cggtcatcga
     1261 attcgagaac gactcattag ctgatgtgac ggccgatttc tggcgagcta actatcgact
     1321 ggaagtcgga taccaggact cggaccgcgt cgacgtttgg caaagctacc ttggttcttc
     1381 tgagcgtccg gcgcgtgtcc ccaccaacat ggaagttggt caatcggcga gtggcaccgc
     1441 tgagattttc gccggcggct ctgtcttcgt ggatttctcg cctcctgcca gcgctgggcc
     1501 aacaacactt acggttgaaa caactgctgc cgacggccgc ggtcaggagg atctggaggt
     1561 ttctttcctt ccattcgatg attatcccgg tctttgctca gcaggcattt caagcgggac
     1621 tagctttgag attgatctgg gcgaagattg cgagttcgcg accctggtgt tgacccacac
     1681 aaatcctcga gcggcagtga ccgattctcg tgaagtcaac tggaccgcga ccctgcaaga
     1741 agttccgggg ccgtacgacg aagaagcaaa tgatgactcc cctgcaggct actggcgtct
     1801 cggtgagccg gatcccaatc cggctataga cgagaccgga aacccgtctg gttcgtacag
     1861 aggtaccaag cagggagtgg ccggagcttt gggcgggtca aacaccgcga tcaccaccaa
     1921 cgtatcaacc gatcatgttg tcgtgggcgg acgcaataat ctcaacaact tctccgcgaa
     1981 caagctgacc ctcgaggcct gggtcaagcc cgagtggtcc aagatgggca gtaacgcggc
     2041 catcatctcg aagccgtgga gagacggttc atggaactct ccctggggcc aatacggctt
     2101 ggtacgacta ccgggcacca accggttggt gttcgagttc cgggatccct ccgagggcta
     2161 tcactccgtg acgtcggcaa gctctctcag cgccgacact tggcatcacg tcgccggtac
     2221 cttcgacggt gacaccttgc gcatctacat caacggcgag ctcgaccaca gctccacctg
     2281 gaccggttcg atcgcacaac gcagctcgga cctcgcgatc gggacgcgta gcgattcatc
     2341 gcctggcgaa tcgtgggtcg gagggatcga tgaagtcgcg atctacgcca gcgcgcttgc
     2401 cggcggacga gtggatgccc atcatgacgc ttcagctgcc aactaccgtg gtgaggtgat
     2461 ggccgactcc ccggtcggct actggcgtct cggtgagccc acgccgaatc agtccatcga
     2521 cgaaactggg aatggcaacg gtatctataa aaatgtcgcc caaggtgtgt ccggtgcttt
     2581 agacgattcc aacgcggcta ttacgactag gggtggcgca agtcatgtcg tcatccccgg
     2641 tcgaggcaac atcaacaacc tcactgatga attgacattg gaagcgtggg tcaagcccga
     2701 gtggtccaag atgggtacca acgcagccat catctcgaag ccgtggcggg atggctcatg
     2761 gaattcacct tggggccaat acggattggt ccgactaccg ggctccaacc gattggtgtt
     2821 cgagttccgg gatccatctg agggctatca ctcagtgaca tcggcaactt cgctcagcgc
     2881 cgacacctgg tatcacgtcg ccgggacttt cgatggtgac acgttgcgca tctacatcaa
     2941 cggcgagctc gaccacagct ccacctggac cggatcaatc gcacaacgca gctcggacct
     3001 agcgatcggg acgcgtagcg attcatcgcc tggcgaatcg tgggttggag ggatcgatga
     3061 agtcgcagtg tacgaccacg cggtgtcagc tgaacgggta tcaatgcact tcagagcggc
     3121 atctacggac taggaagtcg caatcagatc ggtgaataga gaggagtgat gcggacaagt
     3181 cttcgtaaag gcaccggaca agtgtttcga cagaaggatg ttcgaaaaca cccacacaag
     3241 tcttcagcga aattttggac ggtcgccgag acgaaagtcc tggtcagagg cttctgagat
     3301 cttcgaaccg cgcaccaatc ttcaaaacgg tggaaggccg aaagcccttc ggcgggttcg
     3361 attcccgtcc gcctccgctt ctgtggttgc tcgcccgcat ggcgggctcc gctcctccgc
     3421 gtccacgagt acgaaagtac gttccctcgc cgtcggggct ggatcgcttt cggatctaaa
     3481 gcgccacgcg aagctagcgc tccaactggt cctcgacctc ctcctcgacc tcctccttcg
     3541 atccctctgc ccagtcctcg gccgccttct tttggttctc gacctccttc ttctgttcgt
     3601 cgacctcctt cttttggtcc tcgatctcct tcttctgttc gtcgatctcc ttcttctgct
     3661 cctcggccca cgtattggtg tcgtcaacga atccgggcgc actgccgtag gtcctgtctg
     3721 cgccgggctt ggttcttgaa tggcccggag gccggcgtcg gatgcttgct gtgggatcga
     3781 gaccgacgtc acgaagctcc gccacggtcg gcaagcgagc gcgttcatcc tgcagcgggt
     3841 tttcgggacc gactgcgggg atcttgccac cgactgcagc tccggcaagg ccgagcgtcg
     3901 ccgccatgac gacgttcgcg accgagttgg ccatcgtggg cgcatgcatc tctaaggcgg
     3961 actccttccg ggcggcgcgg atggccagat aacggagctt gatcgcgatc gccgacggag
     4021 cccacgggcc gagctcctct cgaagtctcg cgcgcgctct cagtcggagg actttgacgc
     4081 ggttgcgagt gcctgcgggt aaaagggcct cacccacttc agcaagcagg acttgccgtt
     4141 ggtcggcgtt cagttgctgc agggccgagc gagtcttatc aagctgagcc cgttgaaggc
     4201 ccctcacatg gacgtcgtcg ttgtgcatcg gaacggggtc gggcaccaac tcgatcctcg
     4261 attccttgcg ccggtggttg tagacgacgc gcatggcgat cgttgccgcg agattccaca
     4321 gtggttggga gtggtcgagc gtcttccagc gcgggtagag acgggcggcg gtttcttgga
     4381 tcacgtcggc gcgaagccat cgctcgaccc ctttagctgc gaggaagcgt tctagtcggc
     4441 gcgcgatctc cggccactct ctctcgaaag agggtccaac ctcctcatcg aaccattccg
     4501 ctcgagtcgc cacgtacccc tccccgcttg gtcgcgtctg ctctgggaga tctctccctt
     4561 cgtgtattac ttctcgggct ccaagtcggt aacgcctcct gccggtgctg cgccagatcg
     4621 tgtcccgttc acgccacacg ctggcttatg tccgacgaaa agatgtaacc ccgtgtctgc
     4681 ttccgtgtgt atctagttga cggacgacga cacgatcggg agccgaggtg agtcggaacg
     4741 cgccatcgac ggaagaagtt ccgggttggg tgatcctcag cccgacagcg gtcccggacg
     4801 cgtggcgatc gcggaccata ccgatggtgc tggtaccact cacacctgcg gaagcgagcg
     4861 agctcctttc ggacgcgacc gtcgagcagg gcatcgcgcc ggccgatctg ccattgatcc
     4921 atctcgtggc aaggggctac agcgctgcag agatctcccg cgagctcggt gtcgcgctgc
     4981 gcaccgttaa cagacacgtc gctcgtctaa gggacgagtt cggagtggcg accatccaag
     5041 aactagcgag cgagctcgcg aggcggggct ttgggtaaca gcaggcggcg catgttggcc
     5101 tgcgactgtc gcaggacggg agatgggaaa acggaatgaa aaaggaaggc aggcatcaca
     5161 tgtgcaaagg aggccaatga tgaagcactc ggttggttgg atgagggccg cgagtcgacg
     5221 agcggtcatg actgcattag ccgtctcggt tgtggggtcc ggattagcag cggcccccgc
     5281 tgctggtgag tcgcctacca aggtcccgaa ggtggtccag gtcaaggacc ctgccggtga
     5341 cgccaacttc gtgaacaacc aaactgagcc gggtgaggag aggaacgatg tgacacccgc
     5401 cgatctgtcg gttcccgaca tcctgagtgt gtggttcacc cacgacgtca agacgatctc
     5461 ggtccacatc cagaccgagg cgccgccgcc gggctcagag tccgcctaca ggttcgcggt
     5521 gacggtcaac cctgagaacc ccgagctctg cctctacctg gaagccttta tccctggacc
     5581 cacttggtcc gggggcgatt actccacccg ggcgatgccc tgctggcccg atggcgtggt
     5641 accggacgag ggaagggtcc ccggagaact cgagatccat gaatcagcag atggcaccgg
     5701 gatcacctca atgacgtttc cgcgctgggc cggtcgcgca ttcgccgacg gtgaggctct
     5761 tgagtctcct tcggcacgga cccaggtcgt gaccggggcg aaaaacggcg ggttggaagg
     5821 aacgttcttg accgcgtatg tagatcgtac gagcttcgga aaaacctacg tggtcacgaa
     5881 tcgttgaaaa gacaggtgct cctagcccta gcgcttgggg tcacggtcgc gcgagggttg
     5941 gccgtggaac cggtggaggc ggcgcaaggg tctctcgaga agcgaccgtt cactgttgtc
     6001 gccgctgtgg acacgggtat caatccgtat cacgtcgact tccgtactcc ggagctgacc
     6061 cagcatccgt cgacgtacat cacaggcttc ccacgatcgg ctgaggctct gaacctgacc
     6121 ctgagtgcgg agacttacga ggaggcgatc gcgcgcgaca gggccaagtg ggactctgtt
     6181 gaccccaaca agttgtactg ggtgcccggc accaacatcg tgggagcgtt cggtcgcatg
     6241 gccgcggccc ccaacttcct ggaccaagat ggccatggaa cggcggtggc gtcggcggcg
     6301 ggaggcaggg tctacggccc gcgatcgcaa gacatcctca tcgcggccgt ggctggtcac
     6361 cccaataact tggaatgggc ggctcggcag ccgtggatag acgccattac caactcttat
     6421 gtgggctgct gtccgcccgt gccccagcat tctcggatgt cgcgagccgc ggtcgagtcg
     6481 ggcaagctcg tgtgctttgc taccggcaac ttcgtcgcac cggtcttgac cgccggcacg
     6541 caggggcctt cctggatcct tagcgttggt gccgcatcac aaaaaacgcg tggcgagcac
     6601 gtgtatacga gcgcgcccgc cgacatactc gggctctcag gagtggagat cgcccagtac
     6661 cagtcgatcg acggtggcga aggtgggtac aactttggga cgagcatggc ggcgcctgcc
     6721 gcgtgcggcc aggccgcacg cacgatctct gaggttcgcc gcgtcctcgg cgactacacc
     6781 gagggggccg cggatggcct ggcgagtggc cgcaagctaa agggcggcta cctcaaggat
     6841 ggctgcctcg atcgcgtcga gttggaggac tcgctccagg ctacggctgt gcccgcggag
     6901 acttctgcgc cggacccgga cgaccctctt gctaatccgg ccccgccggt cgggggtttc
     6961 gtgcggggcg gttacgggat cgtggaccat gactcggcca acacggcgat aggtgttctt
     7021 ctgggccgcg ctccgcgccc ggaacgagct ctcgaagacg catggatcat gcacactgat
     7081 tcgatacgcg atgcccttta cgggccaccc cccgaaccga ttgggtgccc gtagggattt
     7141 cgggcgaatt acgccgcaga gctcacgccc gggctcgagc tcgtccccat cgccctagcc
     7201 gcctttgccc ccgcgctggg cggatgaacc aaggtagtag tcgtcgcctt cgaagacgac
     7261 tttgtagttt cggttggcac cgcgatgacc cgggggaagc gcgaacgtag cgactccgtc
     7321 gccgtcggtc ttgctggatc cgatcaattc cgaatccgag tacagcttga tcgtgcgccc
     7381 cacgatgctg gcggacggcg tgtcgaggtc ggacaggcgc gcccggactc tcctgtcctc
     7441 gtcgtcgccc tcgaccgtga gcgtgagatc cgtgtcttct ttctcgacca cgaacggcgt
     7501 cgtgtcggca tctccttcgt gggcatcgtc tcctccgaag cgcacggtga gctgatagaa
     7561 gcccggcttc tcctcgagtg tcggcatgaa ggcaccgacg ccgtcatcgt ttgtcgtcgc
     7621 actgaagctg cgcggcgact ccgttccggt cagttcaaag ctcagttcct ggcccgcgat
     7681 cggatggcca ttcgagtcgg ttaggcgcgc ttcgaagacg gtcttatccg agaactggcc
     7741 cgaaacgtgg gaggactcga tgaacgacac atcggtcggc tccagggaag gcgacgacgg
     7801 gcggcagccg aacgagctgg tcgcgtgggt tgacagcgac gggggagcgg cgtccggacc
     7861 ggccccccat tccatgctcg gtacctcgct catttgaagc cgcagtgttg ccccgtcgag
     7921 ctcatcctcc gtcagccatg cgcgatcaag cggcgagccg ccgagggtcg cggaggcgac
     7981 gtacttcgcc accggtgacg taccgggcgc ctcgatcacg agatcggatc gacccggccg
     8041 gtgcacaacg gctcgctcga acacagggct cgccgtggcg tacagcggcg cgcccggggt
     8101 catcggatat atccccagca tcgtccacac gagccatccc gatagcgctc cgagatcgtc
     8161 gttgccgggt agcccatcgg tcgtcggcgt gtagagcgac gcggcggcgc gcgcaacggc
     8221 ctgcgttttc cacggcgcgc ccgcatagtt gtagaggaac ggggcttgca agtcgtgctc
     8281 gttgccgggc gcgtactggt tgccgtagta ggcgatgccg aacagcgtcg cctggttctg
     8341 cgccttcggc acgacgaccg gcgcggcggc cgcgccgggg aacgagaaga attcatcgag
     8401 gcgcaggctc acctcgtcgt cgccacccat acgatcgaag aggccgcgca ggtcgtgcat
     8461 cgccagccac gagtattgcc acgaggtccc ttcctggaag ccgtaaccga gctccggcgc
     8521 gaacggttcg gcccacgcgc cggaggcgtc gcgcggccgg atccagcgcg tctccggatc
     8581 gagcaggtgc cggtacccgg tggagcggct caagaggacg tcgtggtccg aggtgcgccc
     8641 gctcgcgtcc gcgatgagag cgagcgcgaa ctcggcgacg ccgaactcca gcgtcgttcc
     8701 ggtctcgcgc gggccgccgt cgacgaggtc ggccgggtct gacggctgcg gcaccggcag
     8761 gtagccgagg cgcaggtagt catcggatcg acgcgacgcg agcgaccaca tcgcgtcgta
     8821 cagctcgtcg gccatcggct cgtcgaccag gccgcgacac caggcctcgg cgatgaacgg
     8881 gatcgcagga tcgcccgaca tcgagccggt attg
//
LOCUS       DATMED010000431         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1891493, whole genome shotgun sequence.
ACCESSION   DATMED010000431 DATMED010000000
VERSION     DATMED010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891493"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VNP73_11310"
     CDS             complement(<1..372)
                     /locus_tag="VNP73_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWL66552.1"
                     /translation="MLKRPGHDAYSGAPAGLIAIAVAAALWAVAAALARVLFDDGVDP
                     LELVQARALLSAAGLALVPAAWRRPKEGGRLRHVIALGLCIALVNAAYYIAIQKLAVA
                     VAIVLQYLAPALVVVWTAVKLR"
     gene            482..1711
                     /gene="sufD"
                     /locus_tag="VNP73_11315"
     CDS             482..1711
                     /gene="sufD"
                     /locus_tag="VNP73_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HWL66553.1"
                     /translation="MSGLTKQAVEALSSALGEPGWLAERRLSAFDIYEKLELPDPKGE
                     EWRYVDVRKFDFDRFSAPEAVEVDHPIPDEIAAAGVVFATLQTALKDHQDLLKEHFFT
                     DVPVDEHKFTALHGAFYNDAVFVYVPRGVEVEIPIQVVRTVGPGASTFPHTSIVVDEN
                     ASLTFVDRFTCGRLDAAALSGSVVEVDARRGASVNYISLQGWSRDVHHFQTQRFTGHR
                     DTTVRSLAVNLGSSFARTQVECVLKGEGSFSEMLGLYFAEADQHFAQRTLQSHNAPHT
                     TSDLLYKGALKERSRSEYSGLIKVLKDAQGTDAYQANRNLVLSHDALARSIPQLEIEA
                     NEVRCTHGATVSPVEEEHLFYLMSRGIDRMTAQKLIVFGFFTDVLDRIRVPEVRAQLS
                     AAIARKVEGEQRLEVAV"
     gene            1713..2033
                     /locus_tag="VNP73_11320"
     CDS             1713..2033
                     /locus_tag="VNP73_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HWL66554.1"
                     /translation="MSEYEPVAKLDEIPDGGMRQFVAHGEPIGLYRVGSDVFALHDLC
                     THEETFLTDGEFEIEDMEVECPLHGSRFDVRTGSVRILPATKPVRAYEVKVEGDVVLV
                     GPEK"
     gene            2047..2850
                     /gene="sufC"
                     /locus_tag="VNP73_11325"
     CDS             2047..2850
                     /gene="sufC"
                     /locus_tag="VNP73_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWL66555.1"
                     /translation="MAENLIQIEDLHVEVDGREILRGLDLTLKKGEIHAIMGPNGSGK
                     STLAYVLTGRPGYEVTSGKVLYNGENVLELAPDERAKRGIFLAFQYPTEVPGVSVVNF
                     MRTAYNAIHADEQKSAMEFRLYLQEKVDLLEIPSELVDRYVNQGFSGGEKKRNEILQM
                     AVLEPELAILDETDSGLDIDALKHVSEGVNKLAGPEVGILLITHYQRLLNYIRPGTVH
                     VLLGGRIVKSGGFELAELLEAQGYAGLAKEFGLSEEEVAEVETKEGAPA"
     gene            2847..>3389
                     /locus_tag="VNP73_11330"
     CDS             2847..>3389
                     /locus_tag="VNP73_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWL66556.1"
                     /translation="MSFDVEVVRKDFPIFDYQPHGKPLVYLDSAATAQKPRQVIDRMV
                     HFYEAENANVHRGVYELGEIATVAYEGARDACARFIGAPSSRTIVFTRGTTESINLVR
                     FSWGRTNISAGDEILLTDMEHHSNLIPWQLLAEEVGATLRHLPIDDSGELRMDLLDEY
                     VTSKTKLVAITLMSNVLGTIN"
BASE COUNT          670 a         1038 c         1075 g          606 t
ORIGIN      
        1 acgcagtttc acggccgtcc acacgacgac gagagcaggg gcgaggtact gcaggacgat
       61 cgcgaccgcg acggcgagct tctggatcgc gatgtagtaa gcggcgttga ccagggcgat
      121 gcagagccct agagcgatga cgtgtcgcag cctgccccct tctttcggcc ggcgccacgc
      181 cgccggaacg agagctaggc cggctgccga cagcagcgcc cgggcctgga cgagctcgag
      241 cgggtcgacc ccgtcatcga ataggacccg ggccagggcg gccgcgaccg cccacagggc
      301 cgccgccaca gcgatcgcga tgaggcccgc cggggccccg gaataggcgt cgtgtccggg
      361 tcgttttagc actctgagca gggtaaacgc gactttttcg gccttggagt taggtgaacc
      421 taactggtag gctagtgaag cctttcacaa gcaggatcga caacggatcg aggcgttctc
      481 ggtgtccgga ttgaccaaac aggcggttga agctctctcc agcgcgctcg gggaaccggg
      541 ctggctggcc gaacgccggc tatcggcctt cgacatctac gagaagctcg agctgcccga
      601 ccccaagggc gaggagtggc gctacgtcga cgtccgcaag ttcgacttcg atcggttcag
      661 cgctcccgag gcagtcgagg tcgatcatcc gatcccggac gagatcgcgg ccgcaggggt
      721 cgtgttcgcg accctacaga ccgcccttaa agaccaccag gacctactga aggaacactt
      781 cttcaccgac gttcccgtcg acgagcacaa gttcaccgcg ctccacggcg ccttctacaa
      841 cgacgccgtg ttcgtctacg tgccgcgcgg ggtggaggtc gagataccga tccaggtcgt
      901 gcgaaccgtc gggcctggtg cctcgacctt cccgcacacc tcgatcgtcg tggacgagaa
      961 cgcgagcctc acgttcgtgg accggttcac ctgtggccga ctcgatgcag ctgcactgtc
     1021 cggcagcgtc gtcgaggtcg acgctcgtcg cggcgcttcc gttaactaca tctcgttgca
     1081 aggctggtcg cgcgatgtac atcacttcca aacgcagcgc ttcaccgggc atcgcgacac
     1141 gaccgtgcgt agcctggcgg tcaacctcgg cagctcgttc gcccgaactc aggtcgagtg
     1201 cgtgctcaaa ggcgaaggct cgttctccga gatgctcggg ctctatttcg ccgaagcgga
     1261 tcagcacttc gctcaacgaa cgctgcaatc acacaacgca ccgcatacga cgtcggatct
     1321 tctctacaag ggcgcactca aagagaggtc tcgctccgag tactcggggc tgatcaaggt
     1381 tctcaaggac gcgcagggaa cagacgcgta ccaagcgaat cgcaatctgg tgctgtctca
     1441 cgacgctctg gctcgttcga tcccgcagct cgagatcgaa gccaacgagg ttcgttgtac
     1501 tcacggcgcg accgtctcgc cggtcgagga ggagcacctc ttctatctga tgagccgcgg
     1561 catcgaccgc atgaccgctc agaagctgat cgtgtttggg ttcttcaccg acgtcctgga
     1621 tcgcatcagg gtgccggagg tacgcgccca gctctcggct gcgatcgcgc ggaaggtcga
     1681 gggcgagcag cgcctggagg tggcggtctg acatgtccga atacgagccc gtggcgaagc
     1741 tcgacgagat ccccgatgga ggcatgcgcc agttcgtcgc tcacggcgag ccgatcggtc
     1801 tctaccgggt cggctcggac gtgttcgcgc tccacgacct gtgtacgcac gaagagactt
     1861 tcctcaccga tggtgaattc gagatcgaag acatggaagt cgagtgcccg ttgcacggct
     1921 cacgcttcga cgtacgcaca ggaagcgtac ggatccttcc ggctaccaag ccggtgcggg
     1981 cgtacgaagt gaaggtcgaa ggcgacgtgg tcctcgtcgg gcccgagaaa tgaggagctg
     2041 atctttatgg ccgagaacct gattcaaatc gaggatcttc acgtagaggt cgatggtcgc
     2101 gagatcctgc gcgggctcga cctgaccctg aagaagggcg agatccacgc catcatgggg
     2161 ccgaacggct ccggaaagtc gactctcgct tacgtactga cgggccgtcc gggttacgag
     2221 gtgacgtccg gcaaggtctt gtacaacggc gagaacgtgc tcgagctcgc tcccgacgag
     2281 cgggcgaagc gcggcatctt cctggcgttc cagtacccga ccgaggtacc aggtgtctcg
     2341 gtcgtcaact tcatgcgaac cgcctacaac gcgatccacg ccgacgagca aaagtcggcg
     2401 atggagtttc gtctttacct gcaggagaag gtcgacctcc tcgagatccc gagcgagctc
     2461 gtcgaccggt acgtgaacca gggcttctcc ggtggcgaga agaaacgcaa cgagatcctg
     2521 cagatggcgg tgctcgagcc cgagcttgcg atcctggacg aaacggactc gggtttggac
     2581 atcgacgcgt tgaagcacgt gtccgagggt gtcaataagc tcgccggtcc cgaggtcggc
     2641 atccttctga tcacgcacta ccaacgcctg ctgaactaca tccggccggg caccgtgcat
     2701 gtcttgttgg ggggccggat cgtcaagtcc ggcggcttcg aattagccga actcctcgag
     2761 gcgcagggtt acgctggatt ggccaaggag ttcggccttt ccgaggaaga ggtcgcagag
     2821 gtcgaaacca aagaaggagc accggcgtga gcttcgacgt ggaagtcgtt cgcaaagact
     2881 tcccgatctt cgactaccag ccgcacggca agccgcttgt ctatctcgat tcggctgcga
     2941 cggcccagaa gccgcgccaa gttatcgacc ggatggtgca cttctacgag gccgagaacg
     3001 cgaacgtgca ccgcggtgtc tacgagttgg gcgagatcgc aacggttgcg tatgaaggtg
     3061 ctcgggacgc atgcgctcga ttcatcggcg caccaagctc tcggacgatc gtcttcacga
     3121 ggggaacgac cgagtcgatc aacctggtga ggttctcgtg gggccgtacg aacatctcgg
     3181 ccggggacga gatcctcctc accgacatgg agcaccactc gaacctgatc ccgtggcagt
     3241 tgctggccga agaggtgggg gccacgctta ggcatctacc gatcgacgac tcgggcgagc
     3301 tccggatgga tcttctcgat gaatacgtga cgtcgaaaac gaagctcgtg gcgatcacgc
     3361 tgatgtccaa cgtgctcggg acgatcaac
//
LOCUS       DATMED010000432         8099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1891516, whole genome shotgun sequence.
ACCESSION   DATMED010000432 DATMED010000000
VERSION     DATMED010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891516"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..752)
                     /locus_tag="VNP73_11335"
     CDS             complement(6..752)
                     /locus_tag="VNP73_11335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66557.1"
                     /translation="MREAHLTLPPLSLNAWLRYDLIERMLDDLPGVTTVLEVGAGQGA
                     IGARLALNLEYTGVEPDAASYERAKQRLDRLGHGRIHRGYVADLPADLTFDLVCAFEV
                     LEHIEDDLGALKEWATRARPGGYVMISSPAWPRRFGSFDRFAGHYRRYTREGYAELLR
                     EAGCEQVKVLTYGFLLGQVLERVRNAATSRKGHLGSMEERTAASGRWGHPARGDRLTK
                     VASAPFRILQRPFVHGDAGTGFVAVGRRMH"
     gene            complement(734..1423)
                     /locus_tag="VNP73_11340"
     CDS             complement(734..1423)
                     /locus_tag="VNP73_11340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66558.1"
                     /translation="MAEPDGIPARMKQDWNERARTDARYFIAKTTDGDAFEASAEEDI
                     VVLLDGLDNILSPQARVLDVGCGIGRLMKPLAARVSEIHGVDVSGEMIQRAHRYLDGF
                     PNVRLFENNGADLSSLDGPYDFVYSYLMFQHIPDRSIVQRYLHEVDRVLKPGGVFRFQ
                     MDGRGDRPFWRAYRAVRSSSSWRGALWTRTAIIDAAQAAGFDVLDCRVDPRKKGLMRY
                     AYVWVTCVKRT"
     gene            complement(1423..2199)
                     /locus_tag="VNP73_11345"
     CDS             complement(1423..2199)
                     /locus_tag="VNP73_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWL66559.1"
                     /translation="MNSETPPSSSPPETDPAWMGTGSMPLDVGVAIQRRRAELGAYNR
                     WIVDEIRPYIGHRVVDVGCGTGNILEFLLDRDRVVGIDIGDEFIELARSRFGDLPNVD
                     LHVADIADVSTLGTLSGERFDSVVCVNVLEHVERDLTALHNMGGLLEPGGRVVLFVPA
                     LPFLHGTMDEVDGHHRRYTKRSLSDAFNGAGFIVERMYYMNLPGMFAWFLDARILRRR
                     FVPASHDGLFQKIVPRIAAIERRIRPPLGLSLLAVGKRAV"
     gene            2245..3306
                     /locus_tag="VNP73_11350"
     CDS             2245..3306
                     /locus_tag="VNP73_11350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWL66560.1"
                     /translation="MEIAYVGPLPPLTGGISQHGARLTDALAALGHSVSRLSWSAQYP
                     KLFYKGPVRDESAGQHPDATFDLRWWDPTSWRRAGKIAGRSQLLVFPWVTPVVAPAYR
                     VLLGAAAPVPAVAVVHNLLPHERRPGDAALARWVLSRVRGVLLHAAADRPTLTAWGIT
                     TDRVAHPPNLPMQRAPQPPAPPHMLLFFGHVRPYKGLDIAIEAVRVLRAKNVPVRLTI
                     AGHVWGSIDDWKRQIAAAGIEDAVSFRPAYVPDEEVAELFASHHLVLAPYRTATQSGI
                     VPLARESGRPVVATMVGGLGEGFRVGVDGELASRPDPIEFAAAIERALRMSPDPGSDD
                     SENVATWADVAAKVVELGR"
     gene            3303..4025
                     /locus_tag="VNP73_11355"
     CDS             3303..4025
                     /locus_tag="VNP73_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66561.1"
                     /translation="MKAIDLIGHVGHRIHIARGREARMLFSWLGDLRGKSLLDVGGGD
                     GYWAAIARRRGAAAFSIDLDPKRTERGLRFTHRPVLLRGDALRLPFAEASFDAVMSIS
                     SIEHFASSEAAIAEMARVLKPHGDLVLSADSLAGRDRWPHLAAAHVRRYAVTAPLDHH
                     LLDEQLVVAGLEPVRSAYLFKSAWSNRLYLELSRHRLAWNVAAPLSPLVAISDRRIGS
                     GSGSIVLIHGRRQPAQEPFVSR"
     gene            complement(3992..5683)
                     /locus_tag="VNP73_11360"
     CDS             complement(3992..5683)
                     /locus_tag="VNP73_11360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWL66562.1"
                     /translation="MKPQPQARVRPAHLALAAILLVALALRVIGSRHGLPVIYNPDEY
                     WHYAWVAINFFETGYDPGYFFNPPAFSYLLHVVFWIALGGGANAQETLSTHPETVFFV
                     ARLTVAALGTLGVWLVYLTGARLFDRGVGLIAAAILAVGPLPVYQSHLAVNDVPMLVP
                     VALSLFGSAGVLRKGRASDYVIAGIGLGLAAATKYTAGIVLIPLLAAAIVRLRADGKI
                     AIGRAALGIAVSIGAFFVANPYALFNFSTFWEQFGGLPTTPSEGSRKLGEPETSGIRY
                     YLWTLTWGLGWIPALAALAGAVMLIRRDKRAATLLLPAPILFILFTGSLLRYFGRYLL
                     PVFPMVVLAAAFAMRHAISATIAKAPRARAVVVGIAVAALLGQGLVRSVHSDFVLTRP
                     DTRNAALAWLDENIPAGSRLQVEPTSRNGWFLAPASVYTRDFKMVGLHPVLARIRGLS
                     PAEMPRRAEDEYVADLEPSLIDAYVEQGICWVVSEGSTSGRARAEPKRVPDAVDYYEE
                     LAERGEVELRVSPYDEPGRAPPFKFDLVAAYYPFEFHRPGAEMTVYRLTKGSCAG"
     gene            complement(5680..6840)
                     /locus_tag="VNP73_11365"
     CDS             complement(5680..6840)
                     /locus_tag="VNP73_11365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWL66563.1"
                     /translation="MKTDPAKTTEPLDLERSAPAEIAPVVGPRDEIRPLTGLRFWAAF
                     WVVTAHFQPVLETLWPAARAFAPISVDGNMGVDLFFILSGFILIYNYRETFARMSPRR
                     YVQFLGLRLARIYPVHLFTLGLFALFVVADELAGPPVVRQTGVFTLVDFGEQLLLIQA
                     WAGDPYSWNQPSWSLSAEWFAYLLFPFLAWAIFRFGSVKALVGTILVGVPAAWLTIEY
                     VAAPPLFPPLLRIGVTFTMGCFLGVVFLNGVGAGRKGWGVLAGGTAVAIIAATQILPD
                     TCCAEYATVIPLMALFIYSLGRAAGPPASWLGTDRAMFWGGASYALYMTHWFVLAVGA
                     VLLPADSFATSSLFVRSLLVLVYLAAFLGAAAATFVFVERPARRRLRDAMAA"
     gene            6912..>8099
                     /locus_tag="VNP73_11370"
     CDS             6912..>8099
                     /locus_tag="VNP73_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWL66564.1"
                     /translation="MGQLQDLSQVPLERLEHEISTLAAHIYAATCRWLRMIAEFDRRE
                     GWTTWGLTSTTEWLAWKCAISPRTAREHVRIARALDDLPRIAEAFAAGELSYSKVKAL
                     SRVANPDNEEELLGIALHATASQLDGLVTRYYRAAGDLDEANRRHRDRFVSWSYDEHG
                     MLRIEARLDPEEGALVAAALESAIGATHHEPPEEGGPAGPQVGDDRSFVQRAADGLVM
                     MAETFLQEGPAARAGGDRYQVVVHLDQGVIDEAVPRDRCHVEDGPALARETLLRLGCD
                     AGVVTPDAGPKTRTIPTSTRRALRARDGGCRWPGCDRKRFVDAHHIRHWIHGGDHVLE
                     NLVQLCRVHHRMVHEGDFTIESAHDGAFTVKTPTGIPIPELSFLRVVGDGIEAQNENR
                     DLSI"
BASE COUNT         1523 a         2588 c         2623 g         1365 t
ORIGIN      
        1 tcacgtcagt gcatgcgacg gccgacggct acgaacccgg taccggcatc cccgtggacg
       61 aacgggcgct ggaggatccg aaagggtgcc gacgcgacct tcgtgagccg gtcgcctctc
      121 gccgggtgcc cccacctccc actcgccgcc gtccgctctt ccatcgaacc aagatggccc
      181 ttgcgtgacg tggccgcgtt cctgacccgc tccagcacct gacccagcaa gaagccgtac
      241 gtgagtacct tcacttgctc gcatccggcc tcacgaagta gctccgcgta gccctcccgc
      301 gtgtaacggc ggtaatggcc ggcgaagcga tcgaaggaac cgaaacgccg gggccaagcg
      361 ggggagctga tcatgacgta tccgcccggg cgggcacgtg tggcccattc cttcaaggcg
      421 ccgaggtcat cctcgatgtg ctcgagaacc tcgaaagcac agacgaggtc gaacgtgaga
      481 tcggcaggga ggtcggccac ataaccccga tggatgcgac cgtgaccgag ccggtccaac
      541 cgctgtttgg cccgctcgta cgaagctgcg tccggctcca cccccgtgta ttccaggttg
      601 agtgcaagtc gcgccccgat ggcgccttgg cctgcgccta cctcgagaac ggtcgtgacc
      661 cccggcagat cgtcgagcat gcgctcgatg aggtcgtacc tcagccacgc attgagtgac
      721 agcggtggga gggtcaagtg cgcttcacgc acgtcaccca cacgtaggca tagcgcatca
      781 aacccttctt gcgcgggtcg acgcgacagt cgaggacatc gaatcccgcg gcttgtgcgg
      841 catcgatgat ggccgtgcgc gtccacagag caccgcgcca gctgctgcta cttctcaccg
      901 cccgataggc gcgccagaag ggacggtctc ctcttccatc catctggaac ctgaagacgc
      961 cgccgggttt cagcacccga tcgacctcat gcagatagcg ttggactatg gaccggtcgg
     1021 ggatgtgttg gaacatcagg tacgagtaga cgaagtcata gggcccatcc aaactactta
     1081 gatccgcacc gttgttctcg aacagccgga cgttcgggaa gccgtcgagg tatctgtgcg
     1141 ctcgctggat catctcgccc gacacgtcga caccgtggat ctcgctgacc cgcgcggcga
     1201 gtggcttcat cagcctgccg atgccgcatc cgacgtccag gacgcgagcc tgcggagaga
     1261 ggatgttgtc gagcccgtcg agcaggacga cgatgtcctc ctctgcagaa gcttcgaacg
     1321 cgtctccatc ggtcgtcttg gcgatgaaat agcgggcatc ggttcgcgcg cgctcgttcc
     1381 aatcctgttt catgcgggcg gggataccgt cgggttccgc catcacaccg ctcgcttgcc
     1441 cacggccaat agcgagaggc cgagcggcgg acggatcctc cgttcgatcg ctgctatccg
     1501 cggcacgatc ttctggaaga gcccgtcgtg cgaggccggg acgaagcggc gtctgaggat
     1561 cctggcgtcg aggaaccagg caaacatccc cggaagattc atgtaataca tgcgctcgac
     1621 gatgaagccg gccccgttga atgcatcgct caaagagcgt ttcgtgtatc tgcggtgatg
     1681 accgtccacc tcgtccatcg tgccgtggag gaaggggaga gccggaacga aaaggacgac
     1741 gcggcccccg ggctcgagca agccacccat attgtggaga gcggtcaggt cgcgctcgac
     1801 gtgctcgagc acattgacgc agacgaccga atcgaatcgc tcgccggaca acgtgcctag
     1861 agtggagacg tctgctatgt cggcgacgtg taggtcaacg ttcgggaggt cgccgaaccg
     1921 tgaccgcgcc agctcgatga attcgtcgcc gatgtcgatc ccgacgaccc gatctctatc
     1981 caggaggaac tccaggatgt tcccggtccc gcagcccaca tcgaccacgc ggtggccgat
     2041 gtagggccgt atctcgtcga cgatccaccg gttgtaggcg ccgagctcgg cgcggcggcg
     2101 ctggatggcc acgccgacat ccagaggcat cgagcccgtc cccatccacg cggggtccgt
     2161 ctccggcggg gacgaggatg gcggagtttc tgaattcacc tgcgcgaagc tagcagttgg
     2221 actcgcctat cctttcgcgc ccgaatggag atcgcatacg tagggcccct gcctccattg
     2281 accggcggca tctcccagca cggtgcgcgg ctcacggatg ctcttgccgc cctgggacat
     2341 tcggtcagtc gactgtcgtg gagcgcgcag tatccgaaac ttttctacaa gggtccggtg
     2401 cgcgacgaga gtgccggaca gcacccggac gcgaccttcg atctccggtg gtgggatcca
     2461 acctcgtggc ggcgcgcggg gaagatcgcc ggtcgttcgc aactccttgt gtttccgtgg
     2521 gtcacccccg tggttgctcc tgcctatcgc gttcttctgg gggccgctgc gccggtgccg
     2581 gcggttgcgg tggtgcataa cctcctgccc cacgagaggc ggcccggcga cgcggcactc
     2641 gcgagatggg ttctgtcgcg agtgcgcggc gtgctgctac acgcggccgc cgatcgaccg
     2701 accctcacgg cgtgggggat aacgaccgat cgtgttgccc atccgccgaa tctgcccatg
     2761 caacgtgccc cacaaccgcc cgcccctccg cacatgttgt tgttctttgg acacgttcgt
     2821 ccatacaaag gtctcgatat cgcgatcgaa gcggtacggg tgcttcgcgc gaagaacgtg
     2881 ccggtccggt tgacgatcgc cggtcacgtc tggggatcga tcgacgattg gaagaggcag
     2941 atagcggccg ccggcataga agacgcggtc tcgttccggc cggcctacgt gcccgacgaa
     3001 gaagttgcgg agttgttcgc gtcacaccac ctcgtgctgg ctccctatcg aaccgcaacg
     3061 cagtcgggca tcgtgccgct tgcgcgggag tcgggacggc ccgtcgtggc gaccatggtc
     3121 gggggtctcg gcgaaggatt cagggttggc gtcgacgggg agctggcatc gcgaccggat
     3181 ccgatcgagt tcgcggctgc gatcgagcgg gcgctccgga tgtcgcccga ccccggctcg
     3241 gacgactcgg agaacgttgc gacctgggcc gatgtcgctg cgaaagtagt cgagctgggc
     3301 cggtgaaagc catcgatctg atcgggcacg tcggccatcg catccacatc gcgcgcgggc
     3361 gcgaggcgcg gatgttgttc tcgtggctgg gggaccttag agggaagtct cttctcgacg
     3421 tggggggcgg cgacggctac tgggccgcga tcgctcgacg taggggagcg gccgctttct
     3481 cgatcgacct cgatccaaag cgcaccgagc gaggcctgcg gttcacccat cggccggttc
     3541 tacttcgcgg cgacgcactt cggttgccgt tcgccgaagc gtcgttcgat gcggtcatgt
     3601 cgatctcttc gatcgagcac ttcgcatcgt ctgaggcggc catcgccgag atggctcgcg
     3661 tgctgaagcc tcacggcgac ctcgtgttgt cggccgacag tctcgccggg cgcgatcgct
     3721 ggccgcatct tgccgcggcc cacgtgcgtc gctacgcggt aaccgctcca ctagaccacc
     3781 atctgctgga cgagcagctg gtggtcgccg ggttggaacc ggtccgctcc gcctatctct
     3841 tcaagagcgc atggtcgaac cgtctctatc tggagctgtc ccggcaccga ttggcatgga
     3901 acgtcgccgc cccgctgtca cctctggttg cgatctccga tcgccggatc ggatcgggct
     3961 cgggcagcat cgtgctgatc cacggtcgcc gtcagccggc gcaggagcct ttcgtcagcc
     4021 ggtagacggt catctccgcc cccggacggt ggaactcgaa cgggtagtag gcagcgacga
     4081 ggtcgaactt gaacggggga gcgcggccgg gctcgtcgta gggcgagacc cgcaactcca
     4141 cttctcctcg ttcggccagc tcttcgtagt agtcgacggc gtccggtact cgcttcggct
     4201 cggctcgggc acgtcctgaa gtcgaaccct cgctcaccac ccaacagatc ccctgctcga
     4261 cgtaagcgtc gatcaagctc ggctcgagat cagcgacgta ttcgtcctcg gcccggcgcg
     4321 gcatctcggc cggagagaga cccctgatcc tggcaagcac cgggtgtaac ccgaccatct
     4381 tgaagtcgcg tgtatagacg gatgcgggcg ccaggaacca accgttgcgg gatgtcggct
     4441 cgacctgcag cctcgagccc gcggggatgt tctcgtccag ccacgccaga gctgcattgc
     4501 gcgtgtcggg gcgcgtgaga acgaagtcgg aatgcacgct tctgaccaac ccttgtccta
     4561 acagcgccgc caccgcgatc cccacgacga cggcgcgcgc ccggggggct ttggcgatcg
     4621 tcgcggagat ggcgtgacgc atggcgaatg cagctgccag caccaccatc gggaagaccg
     4681 gcaacaggta gcggccgaaa tagcgcagca ggcttccggt gaacaggatg aacaggatcg
     4741 gcgccggcag gaggagggtc gccgcacgct tgtcgcgccg gatcagcatc acggcgcccg
     4801 cgagcgcggc gagcgcgggg atccagccca gcccccacgt gagcgtccac aggtagtagc
     4861 ggatcccgct cgtctcgggc tccccgagct tccgagaacc ctctgaaggt gtcgtcggca
     4921 gtcctccgaa ctgctcccag aaggtcgaga agttaaacag cgcgtacggg ttcgccacga
     4981 agaatgcgcc gatcgaaact gcgataccaa gagctgctct tccgatcgcg atcttgccgt
     5041 cggcacgtaa ccgaacgatg gcggcggcca agagcggtat caggacgatg ccggccgtgt
     5101 acttggtggc cgcggcgagc ccgaggccga tgcccgcgat cacgtagtcg gaggctcgcc
     5161 ctttccgcag gactccagcc gatccgaaca gggatagcgc gacgggaacg agcatcggta
     5221 cgtcgttgac ggcgagatgt gactggtaaa cgggaagggg cccaaccgcc aggatcgcag
     5281 ccgcgatcaa tcccactccg cgatcgaaga gtcgtgctcc ggtcaggtag accaaccaga
     5341 cacccaacgt cccgagagcg gcgacagtca gtcgtgcgac gaagaagacc gtttccggat
     5401 gcgtggagag cgtctcctgt gcgttggcgc caccgcccaa agcgatccag aacaccacgt
     5461 ggagcaggta agagaacgcg ggcggattga agaaatagcc ggggtcgtag ccggtctcga
     5521 agaaattgat cgcgacccag gcgtaatgcc agtactcgtc cgggttgtag atgaccggta
     5581 gcccatgacg cgacccgatc accctcagag ctagagccac cagcaggatc gccgccagcg
     5641 ctaggtgggc cggacggacc cttgcctgcg gctgtggttt caagcggcca tagcgtcgcg
     5701 tagccgcctt cgggccggcc gttcaacgaa cacgaaggtt gccgctgccg cgcccaaaaa
     5761 tgcggccagg tagaccaaga ccagcagact ccggacgaag agtgacgagg ttgcgaatga
     5821 atcggccggt aagagaacgg ctcctaccgc caagacgaac caatgtgtca tgtacaacgc
     5881 gtaggaggca ccgccccaga acatcgcccg atctgtgccc agccacgaag caggaggacc
     5941 ggccgcccgg ccgagcgagt agatgaacag ggccatcagc gggatgaccg tcgcatactc
     6001 cgcacagcat gtatcgggga gtatctgggt tgctgcgatt attgcgacgg cggtcccgcc
     6061 ggcaagcact ccccatcctt tccgtccggc ccccacgccg ttcaggaaca cgaccccgag
     6121 gaagcacccc atggtgaagg tgactccgat ccttaacagc ggggggaaga gtgggggggc
     6181 cgcgacgtac tcgatcgtga gccacgctgc aggcaccccg accaagatcg tcccaaccag
     6241 cgctttcacc gagccgaaac ggaagatggc ccacgcgagg aacgggaaca gcaggtacgc
     6301 gaaccactct gcgctcaaag accacgacgg ttggttccac gaatagggat cgcctgccca
     6361 tgcctggatc aagagcaact gctccccaaa gtccaccaac gtgaagaccc ccgtttggcg
     6421 aaccaccggc ggccccgcga gctcgtccgc tacgacgaaa agagcgaaca gacccagggt
     6481 gaagagatgg accggataga tgcgagctag tcgcaaaccg aggaactgca cgtaccggcg
     6541 aggactcatc cgggcgaagg tttccctgta gttgtagatg aggatgaagc cgctgaggat
     6601 gaagaacagg tcgacaccca tgttcccgtc caccgatatc ggcgcgaacg cccgggccgc
     6661 cggccacagt gtctcgagaa ccggttggaa gtgggcggtt actacccaga aggccgccca
     6721 gaaccgaaga cccgtcagtg gtcttatctc gtcccgaggc ccgacgaccg gggcgatctc
     6781 ggccggagca gaccgctcga gatcgagagg ctccgtagtc ttcgctgggt cggttttcac
     6841 gagctccgac cctatttgtg tctcgcccaa gcagtataat cgaacatacg ttcgatatca
     6901 aggaggaagc catgggccag cttcaggacc tctctcaggt accgctcgag cgactcgagc
     6961 acgagatctc aacccttgct gctcacatct acgccgcgac ctgccgttgg ttgcggatga
     7021 tcgcggagtt cgaccggcgg gaaggatgga ccacgtgggg gctgacctca acaaccgaat
     7081 ggctcgcgtg gaagtgtgcg atctcacctc gaaccgcccg cgagcacgtg cggatcgcca
     7141 gagccctgga cgacctgcct cgcatcgccg aggcgttcgc ggccggcgag cttagttatt
     7201 cgaaggtcaa ggccttgtcc cgggtggcga atcccgacaa tgaggaggaa ctccttggga
     7261 tcgcactcca tgcgaccgcg tctcagctcg acggcctggt tacgcgctac tacagagcgg
     7321 cgggcgacct ggacgaagcg aaccgcaggc atcgcgaccg tttcgtctca tggagctacg
     7381 acgagcacgg gatgttgcgg atcgaggccc gactcgatcc ggaggaaggt gccctggttg
     7441 ctgcggccct ggagtcggcg atcggcgcga cccaccatga gccgccggag gagggtggtc
     7501 ccgcgggacc gcaggtcggc gacgaccggt cgttcgtgca gcgggccgcg gacggcctcg
     7561 tgatgatggc cgaaaccttc ttgcaggagg gccccgcagc cagggccggt ggcgatcgct
     7621 atcaggtagt cgttcatcta gaccaagggg tgatcgacga ggcggtcccg cgggaccgct
     7681 gtcacgtcga ggacggaccc gcgctggccc gcgagacgtt gctgcgactg ggatgcgatg
     7741 ccggcgttgt gacgccagac gcgggtccca agacgagaac gatccctacc tcgacgcggc
     7801 gggcgctacg agcgagggat ggtggttgtc ggtggccggg ttgcgatcga aaacgcttcg
     7861 tcgatgccca tcacatccgg cactggattc acggtggtga ccacgtactg gagaatctcg
     7921 ttcagctgtg ccgggtgcac caccgcatgg ttcacgaagg tgatttcacg atcgagagcg
     7981 cgcacgacgg agcttttacc gtaaagaccc cgacgggcat ccccatacct gagctgtctt
     8041 tcctcagagt ggtgggcgac ggcatcgagg ctcagaacga gaaccgcgat ctctcgatc
//
LOCUS       DATMED010000433         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3799755, whole genome shotgun sequence.
ACCESSION   DATMED010000433 DATMED010000000
VERSION     DATMED010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3799755"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VNP73_11375"
     CDS             <1..1000
                     /locus_tag="VNP73_11375"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HWL66565.1"
                     /translation="FNVQPITLAGWFDRLPRSTPHKGLFGVWNHAFPSRHPSVEPEWS
                     RADWYDMVTAWFDRYLKGIDTGVERWPAVQIQSSNGQWWTAKEYPTIGGKAGQLALGP
                     RASLGKKRPKGESMFIEQAQVGGAAPGQEVIFQTPRLKRPLHLTGQPVADLWLRSSRD
                     DAHVAVKLEVLGGNGEPKLHEGSSGEAMATYGVRSMQHLAPMKRGWFEQAQSQATPVD
                     EPFRALVRFLPTDLVVPKGGRLRLTISGSVTYSKGDSLPSGAASQVTVLHKCGRSSTL
                     RFLVPSPRAKLINVREVDEPQPRLKSRPTRVGRKTGGGLATRKICGKGPKRVPFLR"
     gene            1029..1811
                     /locus_tag="VNP73_11380"
     CDS             1029..1811
                     /locus_tag="VNP73_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL66566.1"
                     /translation="MQELWLDDLNAFVRYTDHPGSRPARVYLHGLARSSSTWSGIASD
                     PRLAGHRTVLVDLLGYGLSDKPADFDYRVDSHAGVIERVLDSIRLQRVTVIGHSLGGT
                     IGILLAARRHDLVGVLVVAEANLDPGGGPMSRAIAAQSLEEYKNEGYTKDLERLHGTA
                     AEMHRLASPVAIHRTASSLIELTRPTPREHFVSLDIPRSFLVGEATLGTDPVSGEDGR
                     GLEEAGIEVLVVPEAGHELMLANPEGTVVALVQALKAEDNDG"
     gene            1829..2722
                     /locus_tag="VNP73_11385"
     CDS             1829..2722
                     /locus_tag="VNP73_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66567.1"
                     /translation="MRFTKPLLALALSVLVAAPAAAAPDRAWEEVGRSEIPLLYYQGM
                     THDEEGNRYFSGHLGLFRTDPMLTETGRNDDVLGPEIHLTENYNHIGDLSYYDGRLFL
                     PLECYYPPIGNTCNNGSIGIADPDTLELDFYVKLDPSEIKKAMWCEVSPDGSLLWTQE
                     GDDLLAYDMAELTEANAAPDAEPIHSVVQLPGAVPPSGITGATFIGNELFVAGGSEDG
                     GEIYSIDLRTGESELQTAVEYIGESEGIDDDFDLRKDAAVLPGSLQYMVQPYNEEGYP
                     TNGVTNGVVYHFEKDHPSPKK"
     gene            2781..2984
                     /locus_tag="VNP73_11390"
     CDS             2781..2984
                     /locus_tag="VNP73_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66568.1"
                     /translation="MAEKEIKYRDDSGQEVTDKVDPKTLDKKMNEAETVYGVDGEDKD
                     KAAYLAKEPGEKREEPITGERNS"
BASE COUNT          598 a          948 c          969 g          495 t
ORIGIN      
        1 tttcaacgtg caaccgataa ccctcgccgg gtggttcgat cggttgccac gcagcactcc
       61 ccacaaagga ctctttggtg tgtggaacca cgctttcccc agccgtcatc cctcggtcga
      121 gcccgagtgg agccgcgccg actggtacga catggtgacc gcgtggttcg atcgctacct
      181 gaaggggatc gacaccggcg tcgagcgctg gccggccgtg cagatccagt cttcgaacgg
      241 acagtggtgg acggccaagg aatatccgac gatcggcggg aaggcgggac aactcgcgct
      301 cggcccgagg gcatcgttgg gcaagaagag acccaaaggc gagtcgatgt tcatcgagca
      361 ggctcaggta ggtggcgctg cccccggcca ggaagtgatc ttccaaacgc ctcgcctcaa
      421 gcgtcccctg cacctcaccg gccagccggt tgccgatctg tggcttcgtt ccagtcgcga
      481 cgacgcgcac gttgccgtca agctcgaggt attgggcggt aacggcgagc ccaagctgca
      541 cgaggggagc tccggcgagg cgatggcaac ctacggggtc cgctcgatgc aacacctggc
      601 gccgatgaag cggggatggt tcgagcaagc gcagagccaa gcaacgccgg tcgacgaacc
      661 cttccgcgcg ctcgtccgat tcctgccgac cgatctagtg gttccaaagg gtgggcggct
      721 tcgtctcacg atctcgggat cggtcaccta ctcgaagggt gactcgctgc catcgggagc
      781 tgcctcgcag gtcacggttc ttcacaaatg tggtcggtcg agcacgcttc gcttcctggt
      841 gccctcaccg cgggccaagt tgatcaacgt gcgcgaggta gacgagcccc agccccggct
      901 gaagtcccgc cccacacgcg tcggtcgcaa gacgggcgga ggtttggcca ctcggaagat
      961 ctgtgggaag ggcccgaagc gcgttccgtt ccttcgctag cccggagccc tcctaatatc
     1021 tgaactccgt gcaagagctc tggctggacg acctcaacgc gttcgtccgc tacacagatc
     1081 acccgggcag ccggccggca cgcgtttact tgcacgggct ggcacggtcc tcgtccacgt
     1141 ggtcgggcat cgcatccgac ccgcggctgg ccggacatcg gacggtactc gtcgatctcc
     1201 tgggatacgg actcagcgac aagccggccg acttcgacta tcgggtcgat tcgcacgcgg
     1261 gggtgatcga gcgagtgctg gattcgatcc gtctacaaag agtcaccgtc atcggacaca
     1321 gcctgggagg cacgatcggg atccttctcg cagcacgcag gcacgatctg gtcggcgtgc
     1381 tggtcgtggc cgaggccaat ctcgatcccg gtgggggacc gatgtcgcgc gcgatcgcgg
     1441 cacagagtct cgaggaatac aagaacgagg gatacacgaa ggacctcgag cggttacacg
     1501 gaaccgctgc cgagatgcac cggctcgcgt ccccggtagc tatccatcgc accgcgtcct
     1561 cgctgatcga gctgacccgg ccgaccccgc gcgaacactt cgtgagcctc gacatcccgc
     1621 ggtcgtttct agtgggggag gcaacgctcg gcacggatcc cgtctcgggt gaagacgggc
     1681 gggggctgga ggaggcgggc atcgaggtcc tcgtggttcc ggaggcgggc cacgagttga
     1741 tgctggccaa ccctgaggga accgtggtcg ccctcgtcca agctctgaag gcggaggaca
     1801 acgacggcta acggcgaatc tgcttcacat gcggttcacg aaacctctcc tcgcgctcgc
     1861 cctcagtgtg ctcgtggcgg cccccgcggc ggccgccccc gaccgagcct gggaagaggt
     1921 cggccgtagc gagataccgc ttctctacta ccaagggatg acccacgacg aggagggcaa
     1981 ccgctacttc tccgggcacc tcggcctctt ccgcacggac ccgatgctca ccgagacggg
     2041 acgcaacgac gacgtgctcg ggcccgagat ccacctgacc gagaactaca accacatcgg
     2101 cgacctgtcc tactacgacg gccgcttgtt cctgccgctc gagtgctact acccgccgat
     2161 cgggaatacg tgcaacaacg gctcgatcgg gatcgcagac cccgacacgc tcgagctcga
     2221 cttctacgtc aagctcgatc cgtccgagat caagaaggcg atgtggtgtg aggtgtcgcc
     2281 ggatggttcg ctgctgtgga cacaagaggg cgacgatcta cttgcttacg atatggccga
     2341 gctaaccgag gcgaatgcgg cccccgatgc agagcccatc cattcggtcg ttcaacttcc
     2401 gggcgccgtt ccgcccagcg ggatcaccgg cgcaaccttc atcgggaacg aactcttcgt
     2461 cgccggtggc agtgaggatg gcggcgagat ctactcgatc gatctaagaa cgggagagtc
     2521 cgagctccag acagcggtcg agtacatcgg cgagtctgag ggcatcgatg acgatttcga
     2581 ccttcgtaag gacgcggccg tactccccgg ttcgcttcag tacatggtgc agccctacaa
     2641 cgaggaggga tacccgacga acggcgttac gaacggcgtc gtgtatcact tcgagaagga
     2701 tcacccgagc ccgaagaaat agcgcctcaa gtgggtaagg acagagggta ggtatcaagt
     2761 agcccatctg gaggtgctca atggccgaga aagagatcaa gtaccgggac gactcggggc
     2821 aggaggtcac ggacaaggtc gaccccaaga ccctcgacaa gaagatgaac gaggccgaga
     2881 ccgtctacgg cgtcgacggc gaagacaagg acaaggctgc ctatctggcg aaggagcccg
     2941 gcgaaaagcg cgaggagccg ataacgggcg agcgcaactc ctagactcgg gggcctaagg
     3001 aggccccatg
//
LOCUS       DATMED010000434         2858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2669696, whole genome shotgun sequence.
ACCESSION   DATMED010000434 DATMED010000000
VERSION     DATMED010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669696"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VNP73_11395"
     CDS             complement(<1..334)
                     /locus_tag="VNP73_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWL66569.1"
                     /translation="MEALIQRALDAAVAAGAAYADARGVSASTEALSVNGPSVESLER
                     ADSQGFGVRVLVDGAWGFAASSVLTDEDVSRIALQAVEVGRASATRIAKPVELVPEPV
                     HEARWETAI"
     gene            complement(360..1244)
                     /locus_tag="VNP73_11400"
     CDS             complement(360..1244)
                     /locus_tag="VNP73_11400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWL66570.1"
                     /translation="MATLEDVQERLVDLSDRAGAAVVGIGHRWALGSGVVVAPGRVLT
                     NAHNLRRDEVTVTFADGRSEIGRPAGVDVDGDVAIVEVDTGDAPAIEWNGGSSVGIGT
                     PVFALANPGGRGLRVTFGFVSGTERSFRGPRGRRISGSVEHTAPLLPGSSGGPIVDGS
                     GHFLGLNTNRLGEGFYLAIPADDSLKERVDRLASGTSPRTRRIGVAIAPPEVGRRLRK
                     AVGLPEATGLLVRGVEEGSVAAGAGIQEGDLLIEAAGRALDSADTLFEVLEAAGSSIS
                     LKFLRGVEENSVEVQLDA"
     gene            1280..1720
                     /locus_tag="VNP73_11405"
     CDS             1280..1720
                     /locus_tag="VNP73_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWL66571.1"
                     /translation="MNSNLSVSSPTTPLEEAVGRIGDRWSLLVIDALLGGAKRFNELA
                     DALPAISPNVLSQRLKRLEKEALVVTHPYSRKPLRLRYELTAGGRDLAGALRLLAHWG
                     AGASESVDSPRHELCGTAIEPRWYCPTCARVVDDGEAAAELRYI"
     gene            complement(1717..2553)
                     /locus_tag="VNP73_11410"
     CDS             complement(1717..2553)
                     /locus_tag="VNP73_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66572.1"
                     /translation="MFPNDNHWNLKVDDLSVHKNSDAIIGSIGRDDTLHPDFGAGRWE
                     GARIGIPYVAVPGDQKKVPIRFYYGDESDPGPYPIPKDAPIEGGRDSDGDRHVIVIDR
                     ARCKLYEVFDAHSVDGGSRWRAGSGAVWDLDSNKLRPKGWTSADAAGLPILAGLARYH
                     EVADGEITHALRFTVERSRRAYIYPARHYASDATSADLPAMGQRLRLKKGFDISGFPE
                     QVQVILRALKTYGMIVADNGSDWFISGAPNTRWNDDALHRLKEIEGRNFEVVDTSELP
                     KP"
     gene            complement(2639..>2858)
                     /locus_tag="VNP73_11415"
     CDS             complement(2639..>2858)
                     /locus_tag="VNP73_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HWL66573.1"
                     /translation="AAHINMMIGRYSTLILDSWTRPKYAKLTGRKSVKDATIVRRFKP
                     YGRWAGLAFWLFLTKDWLDETPGGSAES"
BASE COUNT          511 a          973 c          881 g          493 t
ORIGIN      
        1 tgatagccgt ctcccaccgg gcttcgtgca ccggttcggg gaccagctcg acgggctttg
       61 cgatgcgggt cgcgctggcg cgtccgacct ctaccgcctg aagagcgatg cgcgagacat
      121 cttcatcggt cagaaccgac gaagccgcga acccccatgc gccgtcgacg agcacccgaa
      181 ccccgaagcc ctgcgaatct gcacgctcga gggactccac ggatggcccg ttcacggaca
      241 atgcctcagt cgatgccgac acacccctcg cgtcggcata cgccgcgccc gccgccaccg
      301 ccgcgtcgag cgctcgttga atcaacgcct ccattcggag gcgaacctac tccagtcgtt
      361 taggcatcga gttgaacttc gaccgagttc tcttcgaccc cgcgtaggaa cttcaagctg
      421 atcgaggagc cggccgcctc cagcacctcg aacaaggtgt cggccgaatc gagtgcgcgc
      481 ccggcggctt cgatcaacag gtcaccctcc tggatcccgg cccccgcagc gaccgatccc
      541 tcttccacgc cacggaccaa cagaccggtg gcctcgggta ggccaaccgc cttcctcagc
      601 ctgcggccga cctctggggg agcgatcgcg accccgatcc tgcgggtgcg cggggacgtc
      661 ccgctcgcga ggcggtcgac cctctccttc agggagtcgt cggcggggat cgccaggtag
      721 aagccctcgc ccaaacggtt cgtgttcaga cccaggaagt gcccactccc gtcgacgatc
      781 gggcccccgg aggaaccggg aagaaggggg gcggtgtgct cgacgcttcc cgagatcctg
      841 cgaccgcggg ggccgcggaa ggaacgctcg gtgcccgaaa cgaatccgaa ggtcaccctc
      901 aagccgcgtc cgccggggtt cgctagagcg aagaccggcg tgccgatccc caccgacgag
      961 cctccgttcc actcgatcgc gggggcgtcg ccggtatcca cctcgacgat cgcaacgtca
     1021 ccatcgacgt ccacgcccgc cggccggccg atctcgctcc ggccgtcggc gaacgtcacg
     1081 gtcacctcgt cgcggcgcag gttgtgggcg ttagtcagca cccggccggg ggcgacgacc
     1141 acacccgacc ccagagccca gcgatgaccg atgcccacca cggccgctcc ggccctgtca
     1201 ctgaggtcca caagccgttc ctgaacgtct tcgagagtcg ccataatgcc ctcctgacct
     1261 ggtaacttgt tacttgcaaa tgaatagtaa cctaagcgta tcgagcccca ctacccccct
     1321 cgaagaggcc gtgggccgga tcggggatcg gtggagcttg ctcgtgatcg acgccctcct
     1381 tgggggagcg aagcgattca acgagctggc cgacgcactg ccggcgatca gtcccaacgt
     1441 tctgtcgcaa cgattgaaac gcttggagaa ggaagccctc gtcgtgaccc acccgtactc
     1501 ccgcaagcca ctccgcttgc gttacgagct gactgcagga gggcgggatc tcgccggcgc
     1561 ccttaggttg ctcgcccact ggggggccgg ggcatccgag tcggtcgact cgccgcgcca
     1621 cgagctgtgc ggaacggcga tcgagccgcg ttggtactgc ccgacatgcg cacgtgttgt
     1681 tgacgacggt gaagcggctg ccgaactccg ctacatctag ggcttgggca gctcgctcgt
     1741 gtcgacgacc tcgaagttcc tgccctcgat ctccttcaaa cggtgcagcg catcgtcgtt
     1801 ccagcgcgtg ttcggcgcac cgctgatgaa ccaatcggag ccgttgtcgg cgacgatcat
     1861 cccgtaagtc ttcagtgccc taaggatcac ttgcacctgc tcggggaacc cggagatatc
     1921 gaagcccttc ttgaggcgca gccgctgacc catcgcgggc agatcggcgc tggttgcgtc
     1981 cgatgcgtaa tgccgggcgg gatagatgta agcccggcgc gatcgctcga cggtgaatcg
     2041 cagtgcatgg gtgatctcgc cgtctgcgac ctcgtgatag cgggccagcc cggcaaggat
     2101 cgggaggccg gcggcatcgg ccgacgtcca ccccttgggc ctcagcttgt tcgaatccag
     2161 gtcccacact gcgccggatc cggcacgcca ccggctaccg ccatcgacgg aatgcgcatc
     2221 gaacacctcg tacaacttgc aacgcgcgcg atcgatgacg atcacgtggc ggtcgccgtc
     2281 cgagtcgcga cctccctcaa tcggcgcgtc cttcgggatc gggtaagggc ctgggtccga
     2341 ttcgtcgccg taatagaaac ggatcggcac cttcttctgg tcaccgggaa cggcgacata
     2401 cgggatgccg atgcgcgcac cttcccaacg acctgcaccg aaatcgggat gaagggtgtc
     2461 gtcgcgcccg atgcttccga tgatcgcgtc ggagttcttg tgcacggaaa ggtcatcgac
     2521 cttgaggttc cagtggttgt cgttcgggaa cacgcggcag ttgggtgcct cggctaaagg
     2581 atgtgagccg gctggaccta tgaacgcgag cgatgcaacg agcggaacga tgaaggcttt
     2641 agctctcagc tgatcctccc ggagtctcgt ccagccagtc cttcgtcagg aagagccaga
     2701 acgccaaacc ggcccaacga ccgtacggtt tgaatcgacg gacgatcgtt gcgtctttaa
     2761 ccgacttccg ccccgtcagc tttgcatact tgggtcgcgt ccacgagtcc aggatcaacg
     2821 tcgagtaccg gccgatcatc atgttgatgt gggctgcg
//
LOCUS       DATMED010000435         5209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1894029, whole genome shotgun sequence.
ACCESSION   DATMED010000435 DATMED010000000
VERSION     DATMED010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894029"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VNP73_11420"
     CDS             <1..185
                     /locus_tag="VNP73_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66574.1"
                     /translation="RREAEHLWVVNRNDIHSFKDESLLEVAADKDYRFPEAYRDVVVL
                     REKAKERARNLNEAAG"
     gene            182..1015
                     /gene="paaC"
                     /locus_tag="VNP73_11425"
     CDS             182..1015
                     /gene="paaC"
                     /locus_tag="VNP73_11425"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HWL66575.1"
                     /translation="MSDSLIALLIALADDELILGHRHSEWTGFAPYIEEDVAFSSIAQ
                     DEIGHAAALYTRVGALNGDDPDRLGLGRNRSEYRHAVICERSNKDWAYTLARHWLYDT
                     ADSLRLEALEATADAELSALVVKMRREERYHLLHADAWMKRVAEGPVEGRTFLIDALH
                     DAFAESLGLFEPIELEEEAIKEGWLPVPSDELRSRFMQSAGTALDSLGLPTDIPSKLD
                     ERAEFVASSSGDLIASDGSQPVDDQPHGLGGRRGHHTDEFDSLWEDLTHTYREVPGAS
                     W"
     gene            1015..1518
                     /gene="paaD"
                     /locus_tag="VNP73_11430"
     CDS             1015..1518
                     /gene="paaD"
                     /locus_tag="VNP73_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HWL66576.1"
                     /translation="MEPLTKDLETRVWSALQEVKDPEIPAVSVIEMGMVKEVDVDGST
                     VRITVLPTFTGCPAVPMIKEDVTAAVAAVTGVDEVEVTFTYDPPWTTDRITSLGRKKL
                     KDFGLAPPTGNGPVLITEIGLPPTATCPFCASKDTRPENLFGPTPCRAVYYCAACRNP
                     FEQFKQV"
     gene            complement(1523..4678)
                     /locus_tag="VNP73_11435"
     CDS             complement(1523..4678)
                     /locus_tag="VNP73_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWL66577.1"
                     /translation="MLREHDALGAPADWYRKLADVFREVLSEQDLATLLERIATTLGE
                     FVPYEAVSIYEADEEAKALVPLLVRADWSDELKGSIPFGEGITGWVAERREALITGQA
                     HDDPHASTGNPDGAPPSSLIAIPLVAHNSVKGVLNIYRPSDRPFTEDEFELAKRFADA
                     ASLAIDNARAMSFLEQQSLSDPLTSLYNHRHFHERLREELNRASRARDSTSVLMFDID
                     DFKKVNDIHGHSVGDKILVGLADILRETVRGSDVPCRIGGEEFAVILPSCDAGDALGL
                     ASRLLAKVGALHLEETGTITISVGISQGPEHAMNPKELVTYAEAAMLTAKAKGGNRVV
                     LFDDERQDRPGIPSASRGRDVRSVAYLKMLQSLGSKLNRLNNVSQIGKTIVNELRTLI
                     DYHACRIYIAEDDMLVPIAAKGEPASEGAHEAIMRPLRFGEGVTGRAAETAKSLLIPN
                     ALECDFAIHIPGTDEMEESLVAVPLIYNRRVNGVVVISKLGTNQFREEDVRLLEVLAG
                     QASVAMANARLYEAERREAEHAKSLLEFASLISKAPSFGSIGQETVRMAARLLEARQA
                     SLWLRSDTNGQYYCAAHFGYVGDPKVEPIIKHRIDGPVGERFMEGRRGPFVAAPSELE
                     LYFSPLPSGAGHSLAIAPLHSGHGLRGWLAVRHPSDSDGHFPDDQLRLLEGLSFQTSI
                     AMQKAILYKEQKEDAEVANALLEFSRELAQAAGLEEVLARVVELSARILGSPKTAVWM
                     QESSTGELAPQADWGYSDLERARVIELTLERSLAKEFLSPREPFIIKSGGIEGVEGSE
                     FLTVGGTLAVAPMRFDSNVGCISVVAPAYGEYEFSEKKMRLLAGIADQAQLAIANAGS
                     FESLERTFFETVEALANALEAKDEYTSTHARSITDMVLEVGAELGVEGRDLKRLELGA
                     LFHDIGKIGIPSDIIRKQGPLSEEEWDIMRTHPELGEKILAPIDRLADVRVIIRACHE
                     HYDGGGYPDKLKGEDIPLEARIILTCDAYDAMTTDRPYRKRLPHEEAIRRLKEASGTQ
                     FDPRVVDAFLRCFEDKLAAAG"
     gene            4885..5172
                     /locus_tag="VNP73_11440"
     CDS             4885..5172
                     /locus_tag="VNP73_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66578.1"
                     /translation="MDIEVTPEAIEVLKRSLDLAGLSATESGIRLRTAHGLGGGVEIQ
                     VELADGPSEDERTIELDGLKLFVSPAVIEAVPDPVLTVEPQHETVVVRPRS"
BASE COUNT          953 a         1654 c         1627 g          975 t
ORIGIN      
        1 gccgacggga agccgagcac ctctgggtcg tgaatcgcaa cgacatccat tcgttcaagg
       61 acgagtcgct gctcgaggtc gcggccgaca aggactaccg cttccctgag gcctaccgtg
      121 acgtcgttgt tctgcgggag aaggccaagg agcgtgcccg caatctgaac gaggctgcag
      181 gatgagtgat tctctcatcg cactcttgat cgcactggcc gacgatgagc tcatcctcgg
      241 ccaccgccac tccgaatgga cgggcttcgc cccctacatc gaagaggacg tcgcattctc
      301 gtcgatcgcg caggacgaga tcggtcatgc cgccgctctg tacacgcgtg tcggagcgtt
      361 gaacggcgac gaccccgatc gactgggatt gggcaggaac agatccgaat accggcatgc
      421 agtcatctgc gagcgttcga acaaggattg ggcctacacg cttgcccgcc actggctcta
      481 cgacacggcc gactccttgc ggttggaggc gctcgaggcg accgcagatg cggagttgag
      541 cgcgctggtc gtcaagatgc ggcgcgagga gcgttaccac ctgctgcacg cggacgcttg
      601 gatgaagcga gttgcggaag gcccggtcga aggacgcacg ttcttgatag acgcactgca
      661 cgatgccttc gccgagtcgc tcggcctttt cgaaccgatc gagctcgaag aagaggcgat
      721 caaagaaggc tggttgccgg ttccttccga cgagttgcgt tctcgcttca tgcagagcgc
      781 cggaacggcc ctcgattcgc ttgggctccc gaccgacatc cccagcaagc tcgacgaacg
      841 ggccgagttc gtggcgtcgt catccggaga cctgatcgcg agtgacgggt ctcagccggt
      901 cgacgaccag ccccacgggc tcgggggacg gcgcgggcac cacaccgacg agttcgactc
      961 cttgtgggag gacctgacgc acacctaccg cgaggttccg ggggcgtcgt ggtgatggaa
     1021 cctctcacca aggatctcga gacgcgcgtc tggtccgcgc ttcaggaagt gaaggatccg
     1081 gagatcccgg ccgtctccgt catcgagatg gggatggtca aagaggtcga cgtggatggg
     1141 tcaaccgtac ggatcaccgt gctccccacg ttcaccggtt gtccggcggt cccgatgatc
     1201 aaggaggacg tcacggccgc cgttgcggcc gtgaccggtg tggatgaggt cgaggtgacg
     1261 ttcacgtacg accccccgtg gacgaccgat cgcatcacct cgctgggccg aaagaagttg
     1321 aaggacttcg gtcttgcgcc cccgacgggg aacggtccgg tgctgataac tgagatcggg
     1381 cttcccccca ccgccacgtg tcccttctgc gcgagcaagg acacaagacc cgagaacctg
     1441 ttcggcccga ccccgtgccg cgccgtgtat tactgcgccg cgtgtcgcaa cccgttcgag
     1501 cagttcaaac aggtctagag ggttagccgg ccgccgcgag cttgtcttcg aagcaacgca
     1561 ggaacgcatc gaccacgcgc ggatcgaact gagtgcccga ggcctccttg agtcgcctta
     1621 tcgcttcttc gtgcggtaga cgcttgcgat agggacggtc ggtcgtcatc gcgtcgtagg
     1681 catcgcaagt gaggatgatg cgggcctcga gcgggatgtc ctcgcctttc agcttgtcgg
     1741 gatagccccc gccgtcgtag tgctcgtgac atgcacggat gatcacccgc acgtctgcca
     1801 aacggtcgat cggggccagg atcttctcgc ccagctcggg gtgggtccgc ataatgtccc
     1861 actcctcttc gctcaacggt ccttgtttac ggatgatgtc cgacgggata ccgatcttgc
     1921 cgatgtcgtg gaacagggcc cccagctcta gtcgcttgag atcgcggccc tcgacaccca
     1981 gctcggcccc gacctccagc accatgtcgg tgatcgagcg cgcatgtgtg gacgtgtact
     2041 cgtccttcgc ttcgagggca ttcgcaaggg cctcgacagt ttcgaagaac gtccgctcca
     2101 acgactcgaa gctaccggcg ttggcgatcg cgagttgggc ctgatcggcg atcccggcta
     2161 gcagtcgcat cttcttctcg gagaactcgt attcgccata agccggtgca acaaccgaga
     2221 tgcacccgac gttggaatcg aatcgcatcg gagctaccgc cagcgtgcct cctacggtca
     2281 ggaactcgga accttcgaca ccctcgatcc ctccgctctt gatgatgaag ggctcgcgcg
     2341 gactcaagaa ctccttcgcg agcgatcgct cgagggtcag ctcgatgacc ctcgctcgct
     2401 cgaggtccga gtagccccag tcggcttgtg gtgcgagctc gcccgtcgac gactcctgca
     2461 tccacactgc cgttttcggc gaaccgagga tccgggccga gagctcgacc acccgagcca
     2521 gcacctcttc gagtccggcc gcttgcgcca gctcgcgact gaactcgagc aatgcgttcg
     2581 cgacctcggc atcttccttt tgttccttgt agaggatcgc cttctgcata gcgatcgacg
     2641 tctggaagga aagaccctcg agcagtcgga gttggtcgtc ggggaaatgg ccatcggagt
     2701 cactcgggtg cctgactgcc agccagcccc ggagaccgtg gccggaatga agaggagcga
     2761 tcgcaagcga gtgtcccgcg ccggatggaa gaggcgagaa atagagctcg agctcgctgg
     2821 gggccgcgac gaacggtccc cggcgtccct ccatgaagcg ctccccgacc gggccgtcga
     2881 tccggtgctt gatgatcggc tcgaccttcg gatcccccac ataaccgaag tgggccgcgc
     2941 agtagtactg gccgttcgtg tcgctccgca gccacagcga cgcctgccga gcctccagca
     3001 accgcgccgc catccgcacc gtttcttgcc cgatcgaacc gaacgagggt gccttcgaga
     3061 tgagagacgc gaactcgagc agggacttcg cgtgctccgc ttccctgcgc tcggcctcat
     3121 agagccgagc gttcgccatc gcgaccgaag cctggccggc caatacctca agaagccgaa
     3181 cgtcctcctc gcggaattgg ttcgtgccca gcttggagat aacgacgaca ccgtttacgc
     3241 gcctgttgta aatgagcggt acggcgacga gcgactcttc catctcgtct gtccccggga
     3301 tgtggatcgc gaagtcacac tcgagagcgt tcggtatgag cagcgactta gccgtctcgg
     3361 ccgcgcggcc ggtgaccccc tctccgaaac gaaggggccg catgatcgcc tcgtgagcac
     3421 cttccgatgc cggctcacct ttagcggcga tcggaaccag catgtcgtct tccgcgatgt
     3481 agatccggca cgcgtggtaa tcgataaggg tccgaagctc gttgacgatc gtcttgccga
     3541 tctgcgacac gttattaagc cggttgagct tggaccccag actctgcagc atcttgaggt
     3601 aagcgaccga acgaacgtcg cggccccgag acgcagacgg gatccccgga cgatcctggc
     3661 gctcgtcgtc gaagaggacg acacggttac cgcccttcgc cttcgccgtg agcatggccg
     3721 cctctgcata ggtcacgagc tccttggggt tcatcgcgtg ctcgggacct tgcgagatcc
     3781 cgaccgagat cgtgatcgtg ccggtctcct ccaggtggag agcgcccacc ttcgcgagca
     3841 ggcggctggc aagccccagg gcatcgcccg catcgcacga cggaaggatg acggcgaact
     3901 cctcgccgcc gatccggcag ggcacgtccg aaccgcgcac cgtctctctg aggatgtcgg
     3961 ccagaccgac caggatcttg tctcccaccg agtgaccgtg gatgtcgttg accttcttga
     4021 agtcgtcgat gtcgaacatg agcaccgagg tcgaatcccg ggccctgctt gcacggttca
     4081 attcctcgcg cagacgttcg tggaagtggc ggtggttgta gagcgacgtc agcgggtcgc
     4141 tcagtgattg ctgctcgagg aaagacatag ctcgggcgtt gtcgatagcg agcgatgctg
     4201 cgtcggcgaa cctcttggcg agctcgaact cgtcttcggt gaacggccga tccgaggggc
     4261 gatagatgtt caggacgccc ttgaccgagt tgtgggcgac caacgggatg gctatgaggg
     4321 agctcggagg agcaccgtcc gggttgccgg tggacgcatg ggggtcgtca tgggcctgcc
     4381 cggttatgag cgcttcgcgt cgttccgcca cccagccggt gatcccttcc ccgaacggga
     4441 tcgaaccctt cagttcatcg ctccagtcgg cccgcacgag cagcggcact agagccttgg
     4501 cttcctcgtc ggcctcgtag atcgaaacgg cctcataagg gacgaactcg ccgagtgttg
     4561 tcgctatgcg ctcgagaagg gtcgcaagat cctgctcgga aagaacctcg cggaagacat
     4621 ccgcaagttt gcggtaccag tcggcgggcg cgccgagcgc atcatgttcc ctaagcaagg
     4681 aggttgatac cccccacgcg aagccctcct aagtcgtcct ccccaaaccg gtgcggccgg
     4741 tgcttgggat atcggcgccg cccccctgct ccttaatcaa cgtcatactc tcaggaatct
     4801 cacgctgggt gttttcttcc cggtatcttc tgccgagagc agcctgcgcc ctttgaggtg
     4861 cttttgcccg gatcaggagg atcggtggac atcgaggtca ctccggaagc gatcgaagtg
     4921 ttgaaacgct cactcgatct tgccggtcta agcgcgacgg agtccgggat ccgccttcgg
     4981 accgcgcacg gattgggagg aggcgtcgag atccaggtcg agttggccga tggcccgtcg
     5041 gaggacgaaa gaacgatcga gctggacgga ctgaaactgt tcgtctcgcc cgccgtgatc
     5101 gaggccgtgc cggaccccgt tctaaccgtc gaaccgcagc acgaaacggt cgtagtccgc
     5161 ccccgcagct agccgagtgg caactgagcg tcgttatccg acgctgatt
//
LOCUS       DATMED010000436         5653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4575084, whole genome shotgun sequence.
ACCESSION   DATMED010000436 DATMED010000000
VERSION     DATMED010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4575084"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..550
                     /gene="rplF"
                     /locus_tag="VNP73_11445"
     CDS             2..550
                     /gene="rplF"
                     /locus_tag="VNP73_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWL66579.1"
                     /translation="MSRIGQAPVVIPSGVQVSAAAGRVTVNGPKGELQRILPKDITVE
                     VVDNEIIVKRAKESREVKALHGLIRSLLANMVQGVTQGFEKALEIHGVGYRAAKQGNG
                     LEIQVGYSHAVKKDAPKGIEFEVPTPTRIVVRGIDKELVGQTAAEIRAIRKPEPYKAK
                     GIRYEGEHIRRKSGKAAKAIGA"
     gene            570..920
                     /gene="rplR"
                     /locus_tag="VNP73_11450"
     CDS             570..920
                     /gene="rplR"
                     /locus_tag="VNP73_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWL66580.1"
                     /translation="MERKQKIQSLRRRHRRVRKKVLGTPDRPRLAVYRSNRHIYAQLI
                     DDFAGRTLAAASTLKDAGSGDPKEQAKAVGTTLAQRAKEAGVDQVAFDRGGFKYHGRV
                     RAVAEGAREGGLSF"
     gene            920..1468
                     /gene="rpsE"
                     /locus_tag="VNP73_11455"
     CDS             920..1468
                     /gene="rpsE"
                     /locus_tag="VNP73_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWL66581.1"
                     /translation="MGRGGSRGGRDDDRSPYEERVVSINRVAKVVKGGRRFSFTALVV
                     VGDGAGQVGVGYGKAREVPAAIQKGVEEAKKSFFKVPMVASTIVHEIVGEDSGGVVLL
                     KPASPGTGVIAGGPVRAVVECAGIKDILSKSLGSDNPINIVHATVRALRGLSTPESVA
                     RRRRKTPEEVAPAYMLRGGDDG"
     gene            1461..1643
                     /gene="rpmD"
                     /locus_tag="VNP73_11460"
     CDS             1461..1643
                     /gene="rpmD"
                     /locus_tag="VNP73_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWL66582.1"
                     /translation="MAKLKVTQVRSAVDKGKKQRGTIRALGLKRLGHSVVHDDKPEIR
                     GMIRAVQHLIEVEEAK"
     gene            1646..2098
                     /gene="rplO"
                     /locus_tag="VNP73_11465"
     CDS             1646..2098
                     /gene="rplO"
                     /locus_tag="VNP73_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWL66583.1"
                     /translation="MKPHEIRPPAGSHQRRNRKGRGESAGKGKTAGRGTKGTRARNTV
                     KTFFEGGQMPLVRRIPKLKGFKPPRRKLYGAVNVTQVGELEGSEIGPDELRAAGLVRK
                     RDELIKILGDGEISRAVTVKAHAFSASARSKIEGAGGSVTVIDRKASE"
     gene            2095..3393
                     /gene="secY"
                     /locus_tag="VNP73_11470"
     CDS             2095..3393
                     /gene="secY"
                     /locus_tag="VNP73_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWL66584.1"
                     /translation="MNLLAAFVNAFKVPDLRTKILFTLLIMAIYRAGSHIPTPGIDVE
                     AAQQFSQQAQGGVFAFINLFSGGALTQMAVFALGIMPYITSSIIMQLLTVVIPKLEAW
                     SKEGETGYKKITQWTRYLTVLLALLQSTGLAFLFHRGQFGQTGIQANLDLIPEFTPPR
                     VALIVLSLTAGTALIMWLGELITQRGIGNGMSILIFTSIISTLPGEGKAILDQKGMGT
                     FLLIIAMGIGIIVAIVLMEQGQRRIPVQYAKRVVGRRMYGGSSTYIPLKVNQSGVIPI
                     IFASSVLYIPTLLQNVIQNETIQNFIDNNLVNPRAATYMILYGVMVIFFAYFYTAITF
                     NPTDVADNMKKYGGFVPGIRPGRQTAEYLDRILTRITLPGALFIAFIALLPSVALAGA
                     NIQALPFGGTSILITVGVTLETMKQIESQLMMRHYEGFLK"
     gene            3407..4045
                     /locus_tag="VNP73_11475"
     CDS             3407..4045
                     /locus_tag="VNP73_11475"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWL66585.1"
                     /translation="MRLIIFGPQGAGKGTQAARIAEKYGIPAIATGDIFRWAIKGGTS
                     LGLKAKEYVDAGRLVPDSVTIGVIDERLQADDATDGFLIDGFPRNVEQAEALDRIVEK
                     QGHSIDAALVIDVPEEVSIRRITGRRVCSECGRNYHVDAPPEKNWTCDFCGGEVIERT
                     DDHEEVAVRERLRLYHEQTEPLKVYYEKKGLLKEIDGEGSPDEVFSRIVGAI"
     gene            4056..4805
                     /gene="map"
                     /locus_tag="VNP73_11480"
     CDS             4056..4805
                     /gene="map"
                     /locus_tag="VNP73_11480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWL66586.1"
                     /translation="MIYKKSPEEIAIMRRGGKILADVMERLAAAVKPGISTQEIDAIA
                     DQAITAAGAKPSAKGYRGFPGAICTSPNDVIVHGIPSAAVILEEGDIISLDVALLYEG
                     FHVDNARTYPVGTIDSDSALLLKATEEGLEAAIAECRPGNRIGDVGYAVEQVAEGSGF
                     SVVREYVGHGVGRQFHEDPQVPNYGPPGHREVLTPGMTLAIEPMVNAGAGATKLLDDN
                     WTVKTVDGSRSAHFEHTVAITEDGHEVLTRP"
     gene            4960..5214
                     /gene="infA"
                     /locus_tag="VNP73_11485"
     CDS             4960..5214
                     /gene="infA"
                     /locus_tag="VNP73_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWL66587.1"
                     /translation="MAKPKNKEKASSSGKEEGIQVEGTVIEPLPNAMFKVELDNKHQV
                     LAHISGKMRMHYIRILPGDRVLVELSPYDLSRGRVVYRYK"
     gene            5227..5340
                     /gene="rpmJ"
                     /locus_tag="VNP73_11490"
     CDS             5227..5340
                     /gene="rpmJ"
                     /locus_tag="VNP73_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWL66588.1"
                     /translation="MKVQPSVKKVCDKCKVIRRRGRVMVICSNPRHKQRQG"
     gene            5341..>5653
                     /gene="rpsM"
                     /locus_tag="VNP73_11495"
     CDS             5341..>5653
                     /gene="rpsM"
                     /locus_tag="VNP73_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWL66589.1"
                     /translation="MARIGGVDLPRDKRLDIGLTYIYGVGKTKAMETCIGAQVNPSTK
                     VRDLTDEEVVRIRDFIGTNYRIEGDLRREVNQHIKRKIEIGSYQGLRHRRGLPVRGQR
                     TH"
BASE COUNT         1200 a         1622 c         1853 g          978 t
ORIGIN      
        1 gatgtcacgg atcggacaag cgccagttgt gatcccctct ggggtccagg tgagcgctgc
       61 cgcgggccgc gtgacggtga acggtcccaa gggcgagttg caacgcatcc tgcccaagga
      121 cataacggtc gaggtcgtcg acaacgagat catcgtcaag cgagcgaagg aaagccgtga
      181 ggtgaaggcc ttgcacggtc tgatccgatc gttactcgcg aacatggttc agggcgtgac
      241 gcagggtttc gagaaggctc tcgagatcca cggcgtcggt taccgggctg cgaagcaagg
      301 caacggtctg gagatccagg tcggttactc gcatgcggtc aagaaggacg cgccgaaggg
      361 gatcgagttt gaagttccca ccccaactcg gatcgtcgtg agagggatcg acaaggaact
      421 cgtcggtcaa accgcggccg agatcagggc catccggaaa cccgagccgt acaaggccaa
      481 ggggatccgg tacgagggtg agcacatcag acggaagagc ggcaaggccg cgaaggccat
      541 cggagcttag cgagggactg gaaagaagaa tggaacgcaa acagaagatc caatcgctgc
      601 gtaggcgtca ccgccgagtg cgcaagaaag tgctgggcac gcccgatcgg ccaaggcttg
      661 ccgtctacag gtcgaatcgt catatctatg cacagctgat cgacgacttc gcgggacgga
      721 ccttggcggc cgcttcaacg ctcaaggatg cgggcagcgg tgatcccaag gagcaggcga
      781 aggcggtcgg aacaaccctg gcccagaggg cgaaggaagc cggggtcgat caggttgcgt
      841 tcgatcgcgg aggattcaaa tatcacggtc gtgtgcgcgc cgtcgctgaa ggtgcgcgtg
      901 aaggaggctt gagcttctga tgggtagagg aggatcacgc gggggacgcg atgacgatcg
      961 ctctccttac gaggagaggg tcgtatcgat caaccgagtg gcgaaggtgg tcaagggagg
     1021 ccgtcggttc tccttcaccg cgctcgtcgt ggtcggagac ggtgcaggcc aggtaggcgt
     1081 tggttacggc aaggcacgtg aggttccggc tgcgatccaa aagggcgtcg aagaggccaa
     1141 gaagagcttc ttcaaagtgc ccatggttgc cagcaccatc gttcacgaga tcgtcggtga
     1201 ggactccggt ggtgtcgtgc tactcaagcc tgcgtcacca ggaaccggtg ttatcgccgg
     1261 ggggccggtg cgggccgtcg tcgagtgtgc cgggatcaag gacatcctgt cgaagtcact
     1321 tggttcggac aacccgatca acatcgtgca tgcaaccgtg cgtgcgctga ggggtctgtc
     1381 gacgcccgag tcggttgcac gccggcggag gaagacgccc gaagaggtgg caccggcata
     1441 catgttgcgc gggggagacg atggctaagc tgaaagtcac tcaggtgcgc tcggccgtgg
     1501 acaagggcaa gaagcagcgc gggacgatcc gtgccctcgg tttgaagcga ctgggccaca
     1561 gtgtggtcca cgacgacaag cccgagatcc gcggcatgat ccgcgcggtc caacacctca
     1621 tagaggtcga ggaggcgaag taacgatgaa gccgcatgag atcaggcccc cggcgggctc
     1681 ccaccagagg cggaatcgga aaggacgcgg ggagtctgcg ggcaaaggca agacggcggg
     1741 gcgtgggacc aaggggacga gggccagaaa cacggtcaag acgttcttcg agggtggtca
     1801 gatgcccctc gttcggcgca tcccgaaact caagggcttc aagccccccc gcaggaagct
     1861 ctacggggcg gtcaatgtga cccaggtcgg cgagctcgaa gggagcgaga tcggccccga
     1921 cgaactgaga gcggcgggtc tcgttcgcaa gagagatgag ctcataaaga tcctgggtga
     1981 cggcgagatc tcccgggcag ttacggttaa agcgcatgca ttcagcgcat cggctcgtag
     2041 caagatcgaa ggagctggcg gttcggtgac ggtcatcgat cggaaggcct ccgagtgaac
     2101 ctcctcgcgg cgttcgtcaa cgccttcaag gtcccagacc ttcgcacaaa gatcctcttc
     2161 acgctgctga tcatggcgat ctaccgggcc ggatctcaca tccccacacc cggcatcgac
     2221 gtcgaggcgg cgcaacagtt ctcgcagcag gctcaaggcg gggtgttcgc gttcatcaac
     2281 ctgttctcag gcggagcgct gacccagatg gcggtgttcg cgctcgggat catgccgtac
     2341 atcacgtcct cgatcatcat gcagctctta acggtcgtca tcccgaaact cgaggcatgg
     2401 tcgaaggaag gcgagaccgg ctacaagaag atcacccaat ggacgaggta tctcaccgtt
     2461 ctattggccc tgctgcagtc gaccggactt gcattcctct tccaccgggg ccagttcggg
     2521 cagacgggta tccaggcgaa cctggatctg atccccgagt tcacaccacc ccgggtggcg
     2581 ttgatcgtgc tctcgttgac cgccggaacg gccctgatca tgtggctggg cgagctgatc
     2641 acccagcgtg ggatcgggaa cgggatgtcg atcctgatct tcacctcgat catctcgacg
     2701 cttcccggtg aggggaaggc gatcctcgac cagaagggga tgggaacctt ccttctcatc
     2761 atcgccatgg gcatcggcat catcgtcgcc atcgttctca tggaacaggg acagcgacgg
     2821 atccccgttc agtatgcgaa gcgagtggtg ggcagacgga tgtacggcgg atcctccacc
     2881 tacatcccgc tgaaggtgaa ccagtcggga gttatcccga tcatcttcgc gtcgagcgtg
     2941 ctctatatcc cgacactgtt acagaacgtc atccagaacg agaccatcca gaacttcatc
     3001 gacaacaacc tggtgaaccc gcgcgccgcg acgtacatga ttctgtacgg cgtgatggtc
     3061 atcttcttcg cctacttcta tacggcgatc acgtttaacc cgacggacgt ggccgacaac
     3121 atgaagaagt acggcggttt cgtcccgggt atccgtccag gccgccagac cgcggagtat
     3181 ctcgatcgga tcctgacgag gatcacgctg ccgggcgcgc tgttcatcgc attcatcgct
     3241 ttgttgccgt cggtcgcact ggccggcgcc aacatccagg ccttgccgtt cggcggaacg
     3301 tcgatcctga tcacggtggg tgtgaccctc gaaacgatga agcagatcga gtcgcaactg
     3361 atgatgcgtc actacgaggg tttcctgaag tgatcgtgac cgactgatgc gactgatcat
     3421 ctttgggccc cagggggccg gcaaggggac ccaggctgcg cgcatagccg agaagtacgg
     3481 catcccggcc atcgcaaccg gtgacatctt caggtgggcg atcaagggcg ggacctccct
     3541 gggattgaag gccaaggagt atgtcgacgc gggacgtctg gtgcccgaca gtgtgacgat
     3601 cggtgtgatc gacgagcgcc ttcaggccga cgatgctacg gacggttttc tcatcgacgg
     3661 gttccctcgc aatgtggagc aggctgaggc cctcgacagg atcgtggaga agcagggcca
     3721 ctccatcgat gccgcgctcg tgatcgacgt gcccgaggag gtctcgatcc gccgcatcac
     3781 tggtcgtcgg gtctgttcgg aatgcggacg caactaccac gtggatgcac cgcccgagaa
     3841 gaactggacg tgtgacttct gcggcgggga ggtgatagag cgcacagacg atcacgaaga
     3901 ggtcgctgtg cgagaacgct tacgcctcta tcacgagcag accgaaccct tgaaggtgta
     3961 ctacgagaag aaaggtctcc tgaaggagat cgacggcgaa ggttcgccgg acgaagtttt
     4021 ctcccgcatc gtcggagcga tctgagccgg gcccagtgat ctacaagaag tcgcccgaag
     4081 agatcgccat catgcgccgg gggggcaaga tcctggccga cgtcatggaa cggttggccg
     4141 ctgcggtcaa accggggatc agcacccaag agatcgacgc catcgccgac caggccatca
     4201 cggcagccgg ggccaaacct tcggcgaagg ggtatcgcgg tttccccggc gcgatctgca
     4261 cctcaccgaa cgacgtgatc gtccacggca tcccatccgc cgcagtgatc ctcgaggaag
     4321 gggacatcat ctccttggac gtggccttgt tgtacgaggg cttccacgtc gataacgcac
     4381 gcacataccc cgtgggcacg atcgacagcg atagcgctct gctccttaag gccaccgaag
     4441 agggtctcga ggcagcgatc gccgagtgcc gccccgggaa caggatcggt gacgtgggct
     4501 acgccgtcga gcaggtggcc gaggggtccg gcttctccgt ggtcagggag tacgtcggtc
     4561 acggcgtggg ccgccagttc cacgaggacc ctcaggtgcc gaattacggg ccccccggac
     4621 atcgggaggt gctcacgccg gggatgacgc tggccataga accaatggtg aatgcgggtg
     4681 ccggcgccac gaagctcctg gacgacaact ggacggtgaa gaccgtcgac ggcagccgct
     4741 ccgcccactt cgagcacacc gtggccatca ccgaggatgg gcacgaggtc ctcactcggc
     4801 cctgatcagg gcaaacgcgc ttggttcggt tcgaccggct ccgctagctg gtgttactat
     4861 gtcgagccgg tcccggcccc tagaaatgcg cgccccggaa ggggcgtttg ttttgggttg
     4921 ggtcctgtcc aatctcatcg agcgaaggac tttttgcgcg tggctaagcc taagaacaag
     4981 gagaaagcct cttcttccgg caaagaagaa gggatccagg tagagggcac cgtcatcgag
     5041 cctttgccga acgccatgtt caaagtcgag ctggacaaca agcatcaggt cttggcccac
     5101 atctcgggaa agatgcggat gcactacatc cggatcctcc ccggtgacag ggtcctggtc
     5161 gagctgtccc cgtacgacct cagccggggc cgtgtcgtct acaggtacaa gtagttaggg
     5221 atcgggatga aggttcagcc gagcgtgaag aaggtgtgcg acaagtgcaa ggtgatccgt
     5281 cgccgcggtc gcgtgatggt catctgctcg aacccacgac ataagcaaag gcagggatag
     5341 atggcacgaa tcggaggagt cgaccttcca cgggacaagc gtctcgacat cggcctgacc
     5401 tatatctatg gggtcggtaa gacgaaggcc atggagacgt gcatcggcgc acaggtcaac
     5461 ccgtccacga aggtccgcga cctcacggac gaggaagtcg tccggatccg agatttcatc
     5521 gggacgaact accggatcga gggtgacctc cgacgcgaag tcaatcaaca catcaagcgc
     5581 aagatcgaga tcggtagcta ccaagggctg cgtcaccgcc ggggtctccc ggtgcggggg
     5641 cagcggacgc aca
//
LOCUS       DATMED010000437         5419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2674403, whole genome shotgun sequence.
ACCESSION   DATMED010000437 DATMED010000000
VERSION     DATMED010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674403"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VNP73_11500"
     CDS             <1..1197
                     /locus_tag="VNP73_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HWL66590.1"
                     /translation="SRAEELVKLEVENTGKPFALTMSEEIPFMIDNIRFYASAARMLE
                     GKSAGEYMAGHTSFIRREPIGVCAQVTPWNYPMMMAVWKWAPAVAAGNTVVLKPSDTT
                     PVSTVWMAEVLAEFLPPGVVNVITGDRETGAKLVSHHIPDMASITGSVRAGMEVAKAA
                     AADLKRVHLELGGKAPVIVFDDADLESAAENIAVAGYFNAGQDCTAATRVLAGPKIHD
                     DFVAALSEQAKNTKTGDPDDEDVLFGPVNNQNQLGRVMTFLENAPDHAQVVAGGNKVD
                     RNGYFVEPTVVAGLEQKDEMIQNEIFGPVITVQRFDDEEKALAWANDVEYGLASSVWT
                     RDHGRAMRMAKRLDFGCVWINSHIPLVSEMPHGGFKHSGYGKDLSMYGLEDYTRVKHV
                     MSNIEA"
     gene            1202..2542
                     /gene="gabT"
                     /locus_tag="VNP73_11505"
     CDS             1202..2542
                     /gene="gabT"
                     /locus_tag="VNP73_11505"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HWL66591.1"
                     /translation="MPRSIEGPVPGPRSAELFKRREEAIPRAVFNTVPIFIAGGDGAN
                     VLDVDGNRFIDFAGGLGVLNVGRGNPRVMKAVQEQAEAFLHECFHVSLYEGYLELAEQ
                     LNELTPGDHDKKTMLANSGAEAVENAVKVARYATGRQAVMAFSNAFHGRTYMAMTLTA
                     KEIYKKGYAPYAPEVYRVPFAYCYRCPWGLEYPSCGIACAEHTADVIDHEIGHDRLAA
                     MIVEPVQGEGGFVVAPDDYLPALRAICSERDIVLIDDEVQTGMGRTGKLFAIEHYDVV
                     PDIVTTAKSLAAGLPLSGVTGRAELMEAPHVGGLGGTFGGNPVACAAALAVLAELTEG
                     DLLERAEKQARIIRERLDPLEDALGIVGEVRGLGPMVALELVVDEQTKEPNKLATAAV
                     VRHCHQNGVLIMKAGTYDNVVRLLAPLVITDDDLHEGLDILIEGLELANKGALT"
     gene            2573..4027
                     /locus_tag="VNP73_11510"
     CDS             2573..4027
                     /locus_tag="VNP73_11510"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWL66592.1"
                     /translation="MGAPVAVVNPATEEQIADVGSADADQVAEAVTAAKGAFKEWKRT
                     PAAERGAMLHALSAWITEHSDGLAETLTREGGKPLVENKDEMGWSAACFDFYAEIGRL
                     ERGRVVPSGEAGQLNLVIKEPYGVVAAIVPWNYPILLLTWKLAPALAAGNTVVVKPSP
                     YTPLSTLELAQGFAECFPAGVLNVVTGEAETGRALVGHRDVSMIAFTGSVEVGKSVMR
                     SAADQMKKVHLELGGKDAFIVCDDVDLPVAAKGAAWAAYLNSGQVCTSAERFYVMDSV
                     YSDFVDAVVEETKKLVVGDPMADNTDIGPMVRGIQRDKVEAQLVAAQKAGAKVLVGGE
                     RGGFDKGFFLTPAVVVDVDESMDLFRKETFGPVAPIAKVGALTEAIARANDSEYGLGA
                     NVYTRRLDYMLEAMEDLEAGTVWFNDPLTDNEAAPFGGMKSSGGGRELGVEGLEDFRQ
                     AKHIHIDTKMDMKSWWYPYSEYVRHQQEHGTAPG"
     gene            complement(4024..>5419)
                     /locus_tag="VNP73_11515"
     CDS             complement(4024..>5419)
                     /locus_tag="VNP73_11515"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWL66593.1"
                     /translation="STHYAAHKGVSISMAQKLAGRTEEIQAQEIKRYEERTPKSKALF
                     DRAEASMPFGVSSSFQAGDPYPLYLTEGHGSKVTDIDGNELIDYHNGFGTMVVGHAHP
                     KVRAAIEEAAATGTHFAATTEAAVELAEEIKRRFGVEKVRFTNSGTEATMDAVRLGRA
                     ASGKDVLLKIEGSYHGHHDAVMYSVIPTMDSSGPNERPWSVPFSRGIPKETADSTMIV
                     PFNDAPALESLLEEHGDRIGAMIMEPVMMNIGIVVPLPGYLERVRELCDKHSVVLIFD
                     EVKTGVTISPGGATSLYGVQPDLVCLAKAVGGGTPLGAFAGKAAIMDEIESGVAALGT
                     FNGNPLSMKAALATLTEVLTDDAYNLLAKLGTRLAHGCQKALEEYGIEGHATDLGCKG
                     SITFRDKPLERYRDFQEVDDSLFEAFWYWMINRGVYQTPGKEEQWTISVQHSEDDIDR
                     FVDVFADYCRTVTS"
BASE COUNT         1025 a         1663 c         1758 g          973 t
ORIGIN      
        1 tcgcgggccg aggagctggt gaagctcgag gtcgagaaca ccggcaagcc cttcgccctg
       61 acgatgagcg aagagatccc tttcatgatc gacaacatcc gcttctacgc atccgcggcg
      121 aggatgctcg aaggcaagtc cgccggcgag tacatggccg ggcacacttc gttcatccgc
      181 cgagaaccga tcggggtatg cgcgcaggta acgccgtgga actacccgat gatgatggcg
      241 gtctggaagt gggcccccgc cgtggcagcg ggtaacacgg tcgtcctgaa accgagtgac
      301 acgacgcccg tctcaacggt gtggatggca gaggtgctcg ccgagttcct cccgcccggt
      361 gtcgtcaacg tgatcaccgg cgatcgcgag accggcgcaa agctcgtctc gcatcacatc
      421 ccggacatgg cttcgatcac gggaagcgtt cgggccggca tggaggtagc gaaggcggcc
      481 gccgccgatc taaaacgcgt gcacctcgag ctgggaggta aggctcccgt catcgtcttc
      541 gacgacgccg acctcgagtc tgctgccgag aacatcgcag tcgccggtta cttcaatgcg
      601 ggacaggatt gcaccgcggc aacccgcgtt cttgcggggc cgaagatcca cgacgacttc
      661 gtcgcggctc tgtcagagca agcgaagaac acgaagaccg gtgaccccga cgacgaagac
      721 gtcttgttcg gaccggtgaa caaccagaac caactcggtc gcgtgatgac cttcctggag
      781 aacgcacccg atcacgctca ggtagttgcc ggcggcaaca aggtcgatcg caacggctac
      841 ttcgtcgaac cgacggtcgt cgccggtctc gagcagaagg acgagatgat ccagaacgag
      901 atcttcgggc ccgtgatcac cgtccagcgc ttcgacgacg aagagaaggc gctcgcgtgg
      961 gccaacgacg tcgagtacgg actggcttcg agcgtgtgga cgagggacca cggtcgtgcc
     1021 atgcggatgg ctaagcggct cgactttggt tgcgtgtgga tcaactcgca catcccgctc
     1081 gtctccgaga tgccgcacgg gggcttcaag cactcgggct atggaaagga cctctcgatg
     1141 tacgggctcg aggactacac gcgcgtgaag cacgtcatgt cgaacatcga ggcgtagttc
     1201 gatgccgcgc tcgatcgaag gtccggtccc cggtccccgt tctgccgagc tgttcaaacg
     1261 cagggaagag gcgatcccgc gcgcggtctt taacaccgtg ccgatcttca ttgccggtgg
     1321 tgacggagcg aacgtgctcg acgtggacgg caaccggttc atcgatttcg ccggcgggct
     1381 cggtgttctg aacgtagggc gggggaaccc gcgggtgatg aaggccgtgc aggagcaggc
     1441 cgaagcgttc ctccacgagt gcttccacgt gtcgctctac gaggggtacc tggagctcgc
     1501 cgagcagctc aacgagctga cgccgggcga ccacgacaag aagacgatgc tggcgaactc
     1561 cggcgccgaa gcggtcgaga acgcggtcaa ggtcgcaagg tacgcgaccg gaaggcaagc
     1621 cgtgatggcc ttctcgaacg cgttccacgg ccgcacatac atggcgatga cgctcaccgc
     1681 aaaagagatc tacaagaagg gctacgcgcc ctacgcgccc gaggtctatc gggttccgtt
     1741 cgcgtactgc tacaggtgtc cctggggcct ggagtacccg tcgtgtggga tcgcgtgcgc
     1801 ggagcacacg gccgatgtga tcgaccacga gatcggtcac gatcggctcg cggcgatgat
     1861 cgtggagccc gtccagggtg aaggtgggtt cgtcgtagca cccgacgact acttgcccgc
     1921 gctgcgggcc atctgttccg aacgagacat cgttcttatc gacgacgagg tccaaacggg
     1981 gatgggacgc accggcaagc tctttgctat cgagcactac gacgtagtcc cggacatcgt
     2041 caccaccgcc aagtctttgg ccgcggggct cccgctgtcg ggcgtcaccg gccgcgcgga
     2101 gttgatggag gcccctcatg taggcgggct cggtggcacg ttcggcggta atcccgttgc
     2161 gtgtgcggcg gcactcgccg tcctcgccga gttgaccgag ggcgacctcc tcgaacgagc
     2221 agagaaacag gctcggatca tcagagagcg tctcgatccc ctggaggatg cgctcgggat
     2281 cgtgggggag gtccggggtc tggggccgat ggtggcactg gagctcgtcg tcgacgagca
     2341 aaccaaggaa cccaacaaac tcgcgaccgc cgccgtcgtg cggcactgcc atcagaacgg
     2401 ggttttgata atgaaggccg ggacgtacga caacgtcgtg aggctcctgg ctccactcgt
     2461 gatcaccgac gacgaccttc acgaagggct cgacatactc atcgagggtc tagaactcgc
     2521 gaataaggga gcgctcacct agttgcttat cggcggcgaa aaggtagaag gcttgggcgc
     2581 gcccgttgcg gtcgtcaacc ccgcaacgga agagcagatc gcggacgttg gctcggcgga
     2641 cgcggaccaa gttgcggagg cagtaactgc ggccaagggt gcgttcaaag agtggaagcg
     2701 aaccccggcg gccgagcgcg gggccatgct ccacgcattg tcggcgtgga tcaccgaaca
     2761 ctccgacggc ctggccgaga cattgacccg cgagggcggc aagcccttgg tcgagaacaa
     2821 ggacgagatg gggtggagcg cggcctgctt cgacttttac gccgagatcg gccggctcga
     2881 gcgaggacgg gtcgttcctt ccggggaagc cggtcaactc aacctggtga tcaaggagcc
     2941 ttacggggtc gtcgccgcga tcgtcccgtg gaactacccg atccttctgc tcacatggaa
     3001 gctcgcgccg gcgcttgcgg ccggcaacac ggtcgttgtg aagccctccc cctacacgcc
     3061 tctatccaca ctcgagctcg cccaagggtt cgccgagtgc ttccccgccg gggtgctgaa
     3121 tgtggtcacg ggggaggcgg agaccggacg tgcgctggtc ggacaccgcg acgtgtcgat
     3181 gatcgccttt accggttcgg tcgaagtcgg caagagcgtt atgcgttccg cggccgacca
     3241 gatgaagaag gtgcacctgg agctcggggg gaaggacgcg ttcatagtct gcgatgacgt
     3301 cgaccttccc gttgctgcca aaggtgctgc atgggcggcc tatctcaaca gcggtcaggt
     3361 gtgcacgtcg gccgaacgct tctacgtgat ggattccgtc tactcggact tcgtcgatgc
     3421 cgtggtcgag gagacgaaga aactcgtcgt gggcgatccg atggccgaca acacagatat
     3481 cgggccgatg gtgcgcggca tacagcgaga caaggtcgag gcccagttgg tagccgcgca
     3541 gaaagcgggt gcgaaggtcc tcgtcggggg cgagcgcggc ggtttcgaca agggcttctt
     3601 tctgacgccg gccgtcgtcg tagacgtgga cgagtcgatg gacctgttcc gtaaagagac
     3661 gttcggtccg gtcgcgccga tcgcgaaggt cggggccctt accgaagcca tcgcccgggc
     3721 caatgacagc gagtacggcc tgggcgcgaa cgtgtacacg cgccgcctcg actacatgct
     3781 cgaagcgatg gaggacctcg aagcgggaac agtgtggttc aacgatccac taaccgacaa
     3841 cgaagccgcg ccgttcggcg ggatgaagtc gtccggcggc ggtagggagc ttggggtcga
     3901 ggggctagaa gacttccgtc aggccaagca catccacatc gatacgaaga tggacatgaa
     3961 gtcgtggtgg tatccgtact cggagtacgt gcgtcatcag caagagcacg gcaccgctcc
     4021 cggctaggac gtcaccgttc gacagtagtc agcgaacaca tcgacgaacc tgtcgatgtc
     4081 gtcttccgag tgctgcaccg agatcgtcca ctgctcttct ttgccgggcg tctggtacac
     4141 gccacggttg atcatccagt accagaacgc ctcgaagagc gagtcgtcta cttcctggaa
     4201 gtcccggtag cgctcgagcg gcttgtcccg gaacgtgatc gaacctttgc acccgaggtc
     4261 ggttgcatga ccttcgatcc catactcctc cagcgccttc tgacacccat gcgcgagtcg
     4321 tgtcccgagc ttggcgagaa ggttgtacgc gtcgtcggtc agcacctcgg tcagcgttgc
     4381 cagagcagcc ttcatcgaca ggggattgcc gttgaacgtt ccgagtgccg cgacaccgct
     4441 ctcgatctcg tccataatcg ccgctttgcc cgcgaaagcg ccgagaggag tgccaccgcc
     4501 gacggccttc gccagacaca cgagatcggg ctgaacgccg tacaaggacg tcgcgccacc
     4561 cggtgagatt gttacgcccg tctttacctc gtcgaagatc agtacgacgg agtgcttgtc
     4621 acatagctcg cgcacccgct ccaggtagcc cggcagcggg acgacgatgc cgatgttcat
     4681 catcaccggc tccatgatca tcgccccgat cctgtcgccg tgctcctcca acaggctttc
     4741 gagcgcaggg gcgtcgttga acgggacgat catcgtcgaa tcggccgttt ccttcgggat
     4801 cccgcgcgag aacggaaccg accacggtcg ctcgtttggg ccgctcgagt ccatcgtcgg
     4861 gatgaccgaa tacatcaccg cgtcgtgatg accgtgatag gagccttcga tcttgagcaa
     4921 gacgtccttg cccgacgccg ctctccccaa ccgcacggcg tccatcgtgg cttcggttcc
     4981 cgagttcgtg aagcggactt tctcgacacc gaaccgacgt ttgatctctt ccgcaagctc
     5041 cactgccgct tcggtcgtgg cggcgaagtg cgtccccgtc gcggcggctt cctcgatcgc
     5101 ggcccgcacc ttgggatgag cgtgacccac caccatcgtg ccgaaaccgt tgtggtagtc
     5161 gatcagctcg ttgccgtcga tgtcggtgac cttgctgccg tgtccctcgg tcaggtagag
     5221 cgggtagggg tcgccggcct ggaatgagga ggacaccccg aacggcatcg aggcctcggc
     5281 ccggtcgaac aacgccttcg acttcggcgt tcgttcctcg tatcgcttga tctcctgggc
     5341 ctggatctct tctgttcggc ccgcaagttt ctgcgccatg ctgatcgaca cccctttgtg
     5401 tgcggcgtag tgagttgag
//
LOCUS       DATMED010000438         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4198434, whole genome shotgun sequence.
ACCESSION   DATMED010000438 DATMED010000000
VERSION     DATMED010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4198434"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VNP73_11520"
     CDS             complement(<1..116)
                     /locus_tag="VNP73_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006546085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWL66594.1"
                     /translation="MKRVILAAPRGYCAGVDRAVQIVERALDTCGPPVYVRK"
     gene            complement(137..892)
                     /gene="nth"
                     /locus_tag="VNP73_11525"
     CDS             complement(137..892)
                     /gene="nth"
                     /locus_tag="VNP73_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWL66595.1"
                     /translation="MPIDRSAPPETRTPDILKRLNKAYPEATIALDFRSPLECVVSVI
                     LSAQSTDVLVNSVTPALFAKYRKPEDYLKVPEEELQQDIQSTGFFRQKTRSLRGMCQK
                     LLDDFGGEVPTNIADLLTLPGVARKTANVVQSHLWPELVATDPDAGIAIDTHVGRVAV
                     RLGLTQWNSKEAVKIEKDLTALVPRKHWPHVTDWFIAHGRQICDARKPLCGQCPIEHL
                     CPSSQEAGLPDLYRTTAKKKTTVKKPTRRTDPR"
     gene            957..1352
                     /locus_tag="VNP73_11530"
     CDS             957..1352
                     /locus_tag="VNP73_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66596.1"
                     /translation="MTNVTRVLGVVVLGLAAVILWRIMQNPVPVAQGPLPRATAIPFD
                     EPFWGKVRDWEVVGPGTLEVKLRLLNTGNAVDEGECDLTAFDRNGAVASIPVSTGGPA
                     TPSYRVHVTTDIKIPDRGADDIERLEVDC"
     gene            1349..1963
                     /locus_tag="VNP73_11535"
     CDS             1349..1963
                     /locus_tag="VNP73_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWL66597.1"
                     /translation="MSATKLAAPGQSMARSQRRTDMVQAAARLFSERGYHGTSIQHLA
                     DALGLQKGSLYSHIGSKEELLFDVVNEGADRFLERGERALTLQALASVRLKRLLVAHI
                     ETAIEHLDAATVFLNEWRYLSAERRDEIQAKRDRYEEIVRQIITEGIEAGEFRDDANV
                     RFAARLVLSAGNWTYAWFKPGGELGPTEIGERFADLLIRGLQES"
     gene            1960..>2847
                     /gene="paaA"
                     /locus_tag="VNP73_11540"
     CDS             1960..>2847
                     /gene="paaA"
                     /locus_tag="VNP73_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HWL66598.1"
                     /translation="MTYEEKLAHFNEHIETGGKVETEDWMPDDYRRGVLKFIEMHANS
                     EIMGALPERECLPRAPSLMRKMSLAAKIQDEVGHAQLLYRVAEDLGKSRDSMFDDLVN
                     GRSKFHNVFHYPVSHWGDAALIGWLIDGAALVTQASLLDSSYAPYTRVLKRICTEEQL
                     HLRHGEHITLELATGTDKQRWMFQDALDRWWLAIIHFFGPRSNVEKDLLLRWKVKTRT
                     NEDQRQEFLDRYVPRIYELGFTLPAPTPYKGEAHWVINEDDIDWEPLEKIKRNEGPET
                     ARRLTLRKQIYDDHRWVREA"
BASE COUNT          556 a          880 c          912 g          499 t
ORIGIN      
        1 tcttttcgga cgtacacggg ggggccgcag gtgtcgaggg cccgttcgac gatctgaacg
       61 gccctatcca cgccggcgca atacccccgc ggggcggcca agatgaccct cttcataagg
      121 ccacgctagc agcgcctcag cgggggtccg tccttctggt tggcttcttg accgtcgtct
      181 tcttcttggc cgtcgttcga tagaggtcgg ggaggcccgc ttcttgggac gacgggcaca
      241 ggtgctctat cgggcactgc ccacacaacg gcttgcgcgc atcgcagatc tgacgaccgt
      301 gcgcgatgaa ccagtcggtg acatggggcc agtgcttgcg gggaaccaac gccgtcaggt
      361 ctttctcgat cttcacggct tccttcgagt tccactgcgt taggccgagg cgcaccgcca
      421 cccggcccac atgcgtgtcg atcgcgatcc cggcatcggg atcggtcgcc accagctcgg
      481 gccacaggtg actctggacc acgttggcgg tcttgcgggc tacgccgggc aaggtcagca
      541 ggtcggcgat gttcgtcggg acctctccac cgaagtcgtc gagcaacttc tggcacatcc
      601 cccgcaacga cctcgtcttc tgtctaaaga agcccgtcga ctggatgtcc tgctggagct
      661 cctcctcggg aaccttcagg tagtcctcgg gcttgcggta cttggcgaag agcgccgggg
      721 tcacgctgtt caccaggaca tcggtcgact gggccgacag gatgacggag accacgcact
      781 ccaggggcga ccggaaatcc agggcgatcg tcgcctcggg ataggccttg ttcaggcgct
      841 tgaggatgtc cggcgtgcgg gtctcagggg gcgcagagcg gtcgatcggc acgaccgcag
      901 gatatttggg ctatcctact aacgaacggt cgttcgccac gggagagggg ccccagatga
      961 cgaatgtcac cagagttcta ggggttgtcg tattggggct ggcagcggtg atcctgtgga
     1021 ggatcatgca gaacccggta cccgtcgcgc agggtccgct cccgcgagca accgcgatcc
     1081 ctttcgacga gccgttttgg ggaaaggtgc gtgattggga agtggtcgga cccgggaccc
     1141 tcgaagtgaa gctgcgcctc ttgaatacgg gaaacgccgt cgacgagggc gagtgtgatc
     1201 tcaccgcctt cgaccgaaac ggcgccgtcg cctcgatacc cgtttccacc ggcggtccgg
     1261 cgacgcccag ctatcgcgtg cacgttacga ccgatatcaa gatccccgac cgcggagccg
     1321 acgacatcga acggctcgag gtggattgtt gagcgccacc aagctcgcgg cccccggaca
     1381 gagcatggcg cgcagccagc gtcgcaccga catggtgcag gccgcggcgc gactgttctc
     1441 agaacggggt taccacggaa catcgatcca gcatctggcc gacgcgctgg gtcttcagaa
     1501 agggtccctc tactcacaca tcggctctaa agaggagttg ctgttcgacg tggtaaacga
     1561 gggagccgat cggttcctgg aacgaggcga acgggcgctc acgctccaag cgctcgcatc
     1621 tgtgagattg aagcgtttgc tcgttgcgca catcgagacc gccatcgaac acctcgacgc
     1681 cgcaaccgtg ttcttgaacg agtggagata cctgtctgcg gaacgccgtg acgagatcca
     1741 ggccaagcgc gaccgatacg aagagatcgt ccgccagatc atcactgagg ggatcgaagc
     1801 cggcgagttc cgagacgacg ccaacgttcg cttcgccgca cggctcgtgc tgtcggccgg
     1861 aaactggacg tacgcgtggt tcaaacccgg cggcgagctg ggaccgaccg agatcggcga
     1921 acgattcgcc gacctactca taagggggtt acaagagtca tgacgtacga ggagaaactc
     1981 gctcacttca acgagcacat cgagacgggc ggcaaggtcg agaccgaaga ctggatgccc
     2041 gacgactacc ggcgcggtgt tttgaagttc atcgagatgc acgcgaactc cgagatcatg
     2101 ggagcccttc ccgagagaga gtgtctgccg cgtgcgccgt ccctcatgag gaagatgtcg
     2161 ctggcggcga agatccagga cgaggtaggc cacgcgcagc tcctctacag ggtcgccgaa
     2221 gatctcggga agagccgaga ctcgatgttc gacgacctcg tcaacgggcg ctccaaattc
     2281 cacaacgtgt tccactaccc ggtcagtcac tggggtgacg ccgccctgat cggatggttg
     2341 atcgacggcg cagccttggt gacccaggcg tctctgttgg actcgtccta tgccccctac
     2401 acccgagtgc tgaagcgcat ctgcacggaa gagcaactgc acctccgaca cggcgagcac
     2461 atcacgctcg agctggctac cggcacggac aaacagcgct ggatgttcca ggacgcgctg
     2521 gacagatggt ggctcgcgat catccatttc ttcggtcccc gctcgaacgt cgagaaagat
     2581 ctgctgctcc ggtggaaggt gaagacccgt acgaacgagg accagcgtca ggagtttctc
     2641 gaccggtacg ttcctcgcat ctacgagctc gggttcacgc tcccggcgcc gacgccttac
     2701 aagggcgagg cccactgggt gatcaacgag gacgacatcg actgggagcc gctcgagaag
     2761 atcaagcgca acgaggggcc cgagacggcg cgcaggctca cgctgcgaaa gcagatctac
     2821 gacgatcacc gctgggtgcg cgaggct
//
LOCUS       DATMED010000439         3892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3804850, whole genome shotgun sequence.
ACCESSION   DATMED010000439 DATMED010000000
VERSION     DATMED010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804850"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1105)
                     /locus_tag="VNP73_11545"
     CDS             complement(59..1105)
                     /locus_tag="VNP73_11545"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HWL66599.1"
                     /translation="MSERSWLILADGYLEERAAKTAHGVLRYSKDRVAAVLDRANAGR
                     SVLEVLPDIGRDAPIVGSLAEGLAFEPTSLLIGVATPGGWMPTEWRQWVVDAIDAGLE
                     IVNGLHQFLKDDEEFSRLAEEKGVRLWDVRDPPAGIPLFSGKVLDAPHKIVLTVGSDC
                     AVGKKTAAIELALAGEKAGTRTEFVPTGQTGILIAGRGIAIDRVISDFTSGACEQLVL
                     ESDPDSEVLVVEGQGGLWHPAYSGVTLSLLHGTAPHVLVLCHQAGREAIEEPPFTKLP
                     SLDEMVGTYERLGSINRSTKVACVTVNTHGLDDGEARRHIEDAERVTGLPAGDVLRGD
                     APRLWDAVASALGS"
     gene            complement(1102..2130)
                     /locus_tag="VNP73_11550"
     CDS             complement(1102..2130)
                     /locus_tag="VNP73_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HWL66600.1"
                     /translation="MPADWSRESWELEVTQLDLELAERFTIARETWTVARNVFVTLRF
                     ASVSGVGEVNPDPYLGETVDSVAAQLEAIDPRSLENPFNLEGILEILPAGSARAALDI
                     ALHDLAGKIAGLSITKLLGVKGRVPAPTSLTLPIAEIDKMVERARSLADHPVLKMKVG
                     FDGDVDAVSKVREVFPGTLRIDANTGWNEEEAKKRLKELEALDIELCEQPIPIGDYDA
                     LGRVTESTSIPVFADEDVKTSRDVAALAGVVDGVNLKLRKTGGLREAMRAIATARAHD
                     LKVMLGTDLVSGVAATAEAHLAPLVDHLDIDGPLLLKEDPFPGVTYDKGRITVPDLPG
                     LGVRGAPA"
     gene            complement(2140..3117)
                     /locus_tag="VNP73_11555"
     CDS             complement(2140..3117)
                     /locus_tag="VNP73_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWL66601.1"
                     /translation="MAGDQLFELRRTTFNTPAFRGIEFIEVEAKSIINQVPGSYLPFN
                     WTINPYRGCSHACVYCLARKTHTYLEMNAGKDFDSKIVVKVNAAQLLRKELRSKRWKG
                     EGIAMGTATDPYQRAEGKYRLMRGIIETLIEYRNPFSILTKGTLILRDLDLLKEAAQV
                     TDVSTAFSIGTLDEDAWRKSEPGTPHPRKRIEAVAKLNDAGIQCGVLVAPILPGITDG
                     EEQIKEVVKAAIDAGASHVSPILLHLRPIVKEVYMEWLKTTYPDLVPRYLKMYGGSYA
                     SKDEQGSLGARVRRAIDAAGGLKPRIRARGPWRHPREKPPKPTEQLSLL"
     gene            complement(3154..>3892)
                     /locus_tag="VNP73_11560"
     CDS             complement(3154..>3892)
                     /locus_tag="VNP73_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWL66602.1"
                     /translation="VAVVSRRSLKTLPRSGWFTASVMGAFLTAGYIFQTFGLEYTTAS
                     HAGFITGLFVVLTPIFGAMFFKQKVDRAAWIASGASAIGLYLLSGLSGEGSLFGDALV
                     FGCACSFAFHILVTDKGVKGYPIGALLAVQLGVCGLFSIVVAGIQRDLVVPDSSTVWS
                     ALLVTSLFASALGFFVQTYAQKEATPARTALILASEPAFAGLFAYLLNGESLTPLGWA
                     GAGLIMAAILTVEARPYLPFLRPLPER"
BASE COUNT          722 a         1225 c         1215 g          730 t
ORIGIN      
        1 aggaggcagc gcagtgttgc gtgatgggca gcggcagcgt gaccgtcttc tacgggcgtt
       61 agctgccgag agcagacgca accgcatccc acaatcgggg cgcgtcaccc ctcagcacgt
      121 cacctgcagg taaccccgtg acacgttccg cgtcttcgat gtgacgccga gcctcgccat
      181 cgtcgagccc gtgcgtgttc acggtcacgc acgcgacttt cgttgaacgg ttgatcgatc
      241 cgaggcgttc gtaagttccg accatctcgt ccagcgaagg aagtttcgtg aagggaggct
      301 cctcgatcgc ctcgcgcccg gcctgatgac acagcacgag cacgtgcgga gcggttccgt
      361 gtagcaagct cagagtgacc cccgagtaag cgggatgcca caaacctccc tgcccttcaa
      421 cgactaagac ctcggagtcc ggatccgact cgaggacgag ttgttcgcac gcacccgagg
      481 tgaagtcaga gatcacgcga tcgatcgcga tccctcggcc ggcgatgagg atgcccgtct
      541 gtcccgtcgg tacgaactcc gtcctggtgc cggctttctc cccggcgagc gcaagttcga
      601 tcgcggcggt cttcttgccc accgcacagt cgctccccac ggtgagcacg atcttgtgcg
      661 gcgcatcgag caccttcccg gagaacagtg ggatcccggc cggcggatcc ctgacgtccc
      721 acagccggac tcctttctcc tcggccagtc gtgagaactc ttcgtcgtcc ttcaggaact
      781 ggtgcaggcc gttgacgatc tcgagcccgg cgtcgatcgc gtcgactacc cactgcctcc
      841 actccgtggg catccatccg ccgggtgtag caacgcctat gagcagtgaa gtgggttcga
      901 aggccagccc ttcggccaga ctgccgacga tgggcgcgtc gcgtcctatg tcgggaagaa
      961 cctcgagcac ggagcgcccc gcgttggcac ggtccagcac ggccgcgacg cggtccttcg
     1021 agtagcgcaa cacaccgtga gcggtcttcg ccgctcgctc ttcgagatag ccgtcggcga
     1081 ggatgagcca cgagcgctcg ctcacgcggg cgcgccccgg acaccgaggc cgggcaggtc
     1141 cggaactgtg atccgtccct tgtcgtaggt gacgccgggg aacggatcct ccttgagcag
     1201 aagcggccca tcgatgtcga gatgatcgac gagtggggca aggtgagcct ctgccgtcgc
     1261 agcgacgccg gagacgagat cggtgccgag catgaccttc aggtcgtgag cacgagccgt
     1321 cgcgatcgcg cgcatggcct ctcgcagtcc gccggtcttg cgcaacttga ggttcacgcc
     1381 gtcgaccact ccggcgaggg cggccacatc tcgtgacgtc ttcacgtctt cgtcggcgaa
     1441 gaccgggatg gaagtcgact cggtgacgcg accgagagcg tcgtagtctc caatggggat
     1501 cggctgttcg cacagctcga tgtctaaggc ttctagctcc ttcaatctct tcttcgcctc
     1561 ttcctcgttc caccccgtgt tcgcgtcgat cctgagcgtg ccggggaaca cctctctcac
     1621 tttggacacc gcgtccacat cgccgtcgaa gccgaccttc atcttgagga cgggatggtc
     1681 ggcgagcgac cgggctcttt cgaccatctt gtcgatctcg gcgataggaa gggtgagcga
     1741 agtgggggcc gggacgcgcc ccttgacccc gagcagtttc gtgatcgaga gccccgcgat
     1801 cttcccggcc agatcgtgca acgcgatgtc gagcgcggcg cgagcggacc cggcgggaag
     1861 gatctcgagg atcccttcaa gattgaacgg gttctcgagt gacctcggat cgatcgcttc
     1921 gagctgggcg gccacggagt cgaccgtctc tcctagataa gggtcggggt tgacctcacc
     1981 cactcccgac accgaggcga accgaagggt cacgaaaacg ttgcgcgcga cggtccacgt
     2041 ttcccttgcg atcgtgaagc gttcggcgag ctcgaggtcg agttgggtca cctcgagctc
     2101 ccacgactcg cgggaccaat cggcgggcac gccgcaaacc tagagcaggc tgagttgctc
     2161 cgtgggcttt ggcggtttct cgcgggggtg gcgccacgga ccccgcgccc tgatcctcgg
     2221 cttcaaaccg cctgcagcat cgatggcgcg tcgcacgcga gcgcccaaag agccttgctc
     2281 gtctttcgat gcgtaagagc ctccgtacat cttgagatag cgcgggacaa gatccggata
     2341 cgtggtcttg agccactcca tgtagacctc tttcacgatc ggtcgaaggt ggagcaggat
     2401 cggcgacacg tggctggcac cggcatcgat cgcggccttc acgacctctt tgatctgttc
     2461 ttccccgtcg gtgatgcccg gcaggatcgg tgcaacgaga acaccacact ggatccccgc
     2521 atcgttgagc ttcgcaacgg cttctatgcg cttgcgtgga tgaggggtcc ccggctcgct
     2581 cttgcgccat gcgtcctcgt cgagcgtccc gatcgagaaa gcggttgaga catccgtcac
     2641 ctgcgcagct tccttgagca gatccagatc ccgcaggatc aatgtgccct tggtgaggat
     2701 cgagaagggg ttgcgatatt cgatcagcgt ttcgatgatg ccgcgcatca gtctgtactt
     2761 gccctcggca cgctggtagg gatcggtcgc ggttcccatc gcgatgcctt cgcccttcca
     2821 tcgcttggaa cggagttcct tgcggagtaa ttgcgctgcg ttgaccttca cgacgatctt
     2881 gctatcgaaa tctttgcccg cgttcatttc caaataggtg tgagtcttgc gagctaggca
     2941 gtaaacacat gcatgactgc atccgcgata ggggttgatc gtccagttga acgggaggta
     3001 actgcccggg acctggttga tgatcgactt tgcctcgacc tcgatgaact cgatgcctct
     3061 gaacgccgga gtgttgaagg tcgtgcgacg gagctcgaag agctggtctc ctgccatctc
     3121 cacagtatat cgaacgtatg ttcgatagag gtactagcgc tcaggcaagg gcctcaagaa
     3181 gggcaggtag gggcgggcct cgacggtaag gatcgccgcc atgatcaggc ccgccccggc
     3241 ccaccccagc ggggtcagcg actccccgtt cagtaggtac gcgaagaggc cggcgaaggc
     3301 cggttcgctg gcgaggatga gggccgtgcg tgcaggcgtc gcttccttct gagcgtaggt
     3361 ctggacgaag aaacccaggg cggatgcgaa gagcgaagtc accagaagag cgctccagac
     3421 cgtagagctg tcgggaacga caagatcgcg ctgtatgccc gctacgacga tggagaagag
     3481 accgcacacg ccaagctgca ccgccagcag cgcgccgatc ggatagccct tgacgccctt
     3541 gtccgtaacg aggatgtgga acgcgaacga gcacgcgcac ccgaatacga gtgcgtctcc
     3601 gaaaaggctc ccctctccgc tcaacccgga caacaggtag aggccgatcg cggacgcgcc
     3661 ggaagcgatc catgcggcgc gatcgacttt ctgcttgaag aacatcgcgc cgaagatggg
     3721 tgtgagaacc acgaacaggc ccgtgatgaa tccggcgtgt gaggccgtcg tgtactcgag
     3781 cccgaacgtc tggaagatgt aaccggcggt cagaaacgct cccattaccg acgccgtgaa
     3841 ccatccggat ctcggcagtg tcttaagcga cctacgtgac accaccgcga ct
//
LOCUS       DATMED010000440         3962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3043179, whole genome shotgun sequence.
ACCESSION   DATMED010000440 DATMED010000000
VERSION     DATMED010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043179"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VNP73_11565"
     CDS             <1..637
                     /locus_tag="VNP73_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HWL66603.1"
                     /translation="PGVFADVVDEKGEPVGRGGGGYLVLTRPVPSMFRTIYKDDERYR
                     ETYFSKYGDDVYFAGDGAKLDDDGYYWLLGRVDDVMNVAGHRISTYEVESALVDHKAV
                     AEAAVVGRRDPKQGESIVAFVTPKSGEEGTETLMQDLRGHVAQVIGKIARPHSIIFTD
                     DLPKTRSGKIMRRLLRDVAEGRELGDVTTLQNAPILDDIKAKASGLKSQEE"
     gene            714..1628
                     /locus_tag="VNP73_11570"
     CDS             714..1628
                     /locus_tag="VNP73_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HWL66604.1"
                     /translation="MVDEPIVVTEEEVVVTEGAEASLGRRLFAEFVGTLLFVAVGSGA
                     ATVLALGPLRRLGGLTELLQGAPGQDQVFEAILGNSLGDLLPVAFAFATALAVLVYAF
                     GGVSGAHFNPAVTFALAVSNRFKWKEVVPYWVVQILGAVVGAFIIAGIYGQDGASLAG
                     TDILFGATALSDDVNQWQGILGEAFIAFVLMTGIMAVAVDPRAPKGWSGLIIGLALAG
                     GILVTGAATGGSANFARTLGPMVASLPYEVGKIPWGDLWVYAVGPLIGAAAAALVYES
                     VTGLERATPEPAPGAATGESAEESEPVL"
     gene            complement(1663..2187)
                     /locus_tag="VNP73_11575"
     CDS             complement(1663..2187)
                     /locus_tag="VNP73_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWL66605.1"
                     /translation="MSSGKVAFIIDEMFEDSEFRVPYDRVKEAGYEPMIVGLESGKKL
                     EGKKGKEKITTEVSIDEVTPGDFVALVIPGGYSPDKIRSNAKMVELTRSINTAQKPIA
                     AICHAGWMLAEADIISGKTVTSWPSIKTDLINAGAEWVDQEVVEDGNIITSRKPDDLE
                     AFSKTLLAQLEQQN"
     gene            2231..3223
                     /gene="ligD"
                     /locus_tag="VNP73_11580"
     CDS             2231..3223
                     /gene="ligD"
                     /locus_tag="VNP73_11580"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWL66606.1"
                     /translation="MATPTTVSFPIELPTERKGESWYARAGEREIRLSNLDKVFWPLE
                     RFTKGDLLTYYFNISPVMLPHLIDRPLTLKRMPNGVEGDFFYEKNAPRHTPKWMTRIP
                     VKAEGGTKTINFLAACDVAEMLWVVNLGCIEFHPLHGRGSDQSRPSYAFFDLDPMGGA
                     PWDTVKHVASLVKVVLDRFGIDSYPKTSGATGMQIMIPTGGKYTYDEIRGFVGAVSDL
                     VHKADPETTTVEWVVKQRGRKVFLDVNMNREGANIAAAYSVRPERGATVSTPFDWGEL
                     DDIEPEDWSIETIFDRIATHGDPFLPVAEGPWQDLDAAIKELEAAPRKARYIPR"
     gene            3223..>3962
                     /locus_tag="VNP73_11585"
     CDS             3223..>3962
                     /locus_tag="VNP73_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWL66607.1"
                     /translation="MSLYVGTSGWSYREWKPDFYPADIKQKEWLNFYSTRLSACEINA
                     TFYRLQANETFARWRDSVPDSFRYSIKAHRRLTHSRRIAPEGEGKDFLAAFFRSLEPL
                     GDRLGIILWQFPPYRKRDDDQLEALLAEIGGRRGAFEFRDDSWLNAEIRARLADAGHT
                     MCVADTTGDPPTSLPPGPVAYIRLRHERYSEEQRDGWRKLLADEADKRDVYAFSKHEG
                     VPAADPFAGVGFATWLCNAGPRQVYSDG"
BASE COUNT          732 a         1234 c         1251 g          745 t
ORIGIN      
        1 ccccggggtc ttcgcggacg tggtggacga gaagggagaa ccggtcgggc gcggcggagg
       61 cggttatctc gttctcaccc gccccgttcc ttcgatgttc cgcacgatct acaaggacga
      121 cgagcgttat cgggagacgt acttctcgaa gtacggggat gacgtgtact tcgccggcga
      181 cggcgccaag ctcgacgacg acggctacta ctggctgctc ggtcgcgtag acgacgtcat
      241 gaacgttgcc ggtcaccgca tctcgacgta tgaggtcgaa tcggccctcg tcgaccacaa
      301 ggccgttgcc gaagccgcgg tggtcggccg cagggacccc aaacaaggcg aatcgatcgt
      361 cgcgttcgtc acgccgaagt ccggcgagga gggtaccgag acactgatgc aggacctgcg
      421 cggccacgta gcgcaagtga tcggcaagat cgcccggcct cactcgatca tcttcaccga
      481 tgacctcccg aaaacgcggt ccggcaagat catgcgaagg cttctgaggg atgtcgccga
      541 gggccgggag ctgggggacg tcaccacgct ccagaacgcg ccgatcctcg acgacatcaa
      601 ggccaaggcg agcggcctga agagccagga ggaataaagg accacccggg cctaggcaag
      661 taccctgtcc gggccagtac atcaaacaga ccccgactcc tgaaggaggt tcgatggttg
      721 acgagcccat agtcgtcacc gaagaagaag tggtagtcac ggaaggcgcc gaagccagtc
      781 tcggccgacg tctcttcgcc gagttcgtcg gcaccctgct gttcgtggcg gtgggatccg
      841 gcgcggccac cgttcttgca ctgggaccac tgcgcagatt ggggggcttg accgagctcc
      901 tgcaaggcgc accgggtcag gatcaggtct tcgaagcgat cctcggcaac tcgctgggcg
      961 acctgctccc cgtggctttc gcattcgcga ccgcgctggc cgtactcgtt tacgccttcg
     1021 gcggcgtgtc gggagcccac ttcaaccccg ccgtgacgtt cgccctcgcg gtgagcaacc
     1081 ggttcaagtg gaaagaggtt gtgccttatt gggtcgtgca gatcctgggc gcggtcgtgg
     1141 gtgccttcat catcgccggg atctacggtc aggacggagc ctctctcgcc ggcaccgaca
     1201 tcctgttcgg agcgaccgcg ttgtcggatg acgtcaatca atggcagggc atcctcggag
     1261 aagccttcat cgccttcgtg ctgatgacgg ggatcatggc cgtggcggtc gaccctcgcg
     1321 cccccaaggg gtggtccggg ttgatcatcg gtctggctct cgccggcggg atcctggtga
     1381 cgggggccgc cacgggtggt tcggcgaact tcgctcggac cctcggtcct atggttgcat
     1441 cgctgcccta tgaggtcggc aagatcccct ggggagacct gtgggtctac gcggtcggtc
     1501 ctctgatcgg cgctgctgcg gctgcactcg tctacgagag cgtcaccggg ctggagcggg
     1561 cgacgcccga accggcaccc ggagctgcga caggcgaaag tgccgaggag tcagagccgg
     1621 tcctctaatg attgaggggc ctctccttga gaggcccctc tcttaattct gttgctcgag
     1681 ctgcgccagc agagtcttcg agaacgcttc caggtcgtcg ggtttgcgtg acgtgatgat
     1741 gttcccgtcc tcgacgactt cctggtcgac ccactcggcc cccgcattga tcaggtccgt
     1801 tttgatcgag ggccacgagg tgacggtctt accgctgatg atgtcggctt cggcgagcat
     1861 ccaccccgca tggcagatcg ccgcgatcgg cttctgcgcg gtgttgatcg atcgggtcag
     1921 ttccaccatc ttggcgttgg aacggatctt gtccggcgag taaccacccg ggatgaccag
     1981 agcgacgaag tcgccggggg tcacctcatc gatggagacc tccgtcgtga tcttctcctt
     2041 gcctttcttg ccctcgagtt tcttaccgga ctcgagaccg acgatcatcg gttcgtaacc
     2101 cgcttccttc actctgtcgt aggggactcg gaactcggaa tcctcgaaca tctcgtcgat
     2161 gatgaacgcg acttttcctg atgacactgc tccacctcct gtgatcgcct gcgtgtggtt
     2221 tcctaccctc gtggcaacac cgacaaccgt ttcattcccg atagagctgc cgacggagcg
     2281 caaaggtgag agttggtacg cgcgggcagg cgagcgtgag atccgactgt ccaacctcga
     2341 caaagtcttc tggcccctcg agcgcttcac gaaaggcgac ctgctcacct actacttcaa
     2401 tatctccccc gtgatgcttc ctcacctgat cgaccgtcca ttgacgttga aacggatgcc
     2461 gaacggagtg gaaggcgact tcttctacga gaagaacgct cccaggcaca ccccgaagtg
     2521 gatgactcgg atcccggtga aggccgaggg aggcaccaag acgatcaact tcctggccgc
     2581 ttgtgatgtc gccgagatgc tgtgggtcgt caacctcggg tgcatcgagt tccatccgct
     2641 ccacggtcga gggtccgatc agtcgcggcc cagctacgcg ttcttcgatc tcgatccgat
     2701 gggcggcgcg ccctgggaca ccgtcaagca cgtcgccagc ctcgtgaagg tagtcctcga
     2761 ccgcttcggg atcgactcct accccaagac ctcgggcgcg accgggatgc agatcatgat
     2821 ccccaccggc ggcaaataca cctacgacga gatcagaggc ttcgtcgggg cggtttccga
     2881 tctggtgcac aaggccgacc ccgagacgac aaccgtcgaa tgggtagtga agcaacgggg
     2941 tcggaaggtg ttcctcgacg tgaacatgaa ccgtgagggc gccaacatcg cggccgccta
     3001 ttccgtccgg cccgaacgtg gggcgacggt cagcacccct ttcgattggg gcgagttgga
     3061 cgatatagag ccggaggatt ggagcatcga gacgatcttc gatcggatcg caacgcacgg
     3121 ggacccgttc ctgcccgtcg ccgaagggcc gtggcaggac ctcgacgcgg ccatcaagga
     3181 actcgaagct gcgccccgca aggcgcgcta catcccgcgc tgatgtccct ctacgtcgga
     3241 acatccggct ggtcctatcg ggaatggaaa cccgacttct accccgcaga catcaaacag
     3301 aaggagtggc tcaatttcta ttccacgagg ttgagcgcgt gcgagatcaa cgcgaccttc
     3361 tatcgcctgc aggcgaacga gacctttgct cggtggagag attcggtgcc cgactcgttc
     3421 cgttactcga tcaaggctca ccgaaggctg acacacagcc gccggatcgc tccggagggc
     3481 gaagggaagg atttcctcgc tgcgttcttt cgttccctgg aaccgctagg ggaccgcttg
     3541 ggcatcatcc tgtggcagtt cccgccctat cgcaaacggg acgacgatca gctcgaggca
     3601 ctgctcgccg agatcggggg gcgacggggc gcgttcgagt ttcgcgacga ctcgtggcta
     3661 aacgctgaga tacgggcgcg actcgccgac gccggacaca cgatgtgtgt ggccgacacc
     3721 acaggggacc caccaacgtc gctcccgccg gggcccgtcg cctacatacg tctcaggcac
     3781 gagcgctact ccgaggaaca acgtgacggg tggaggaagc tgctcgcgga cgaggcggac
     3841 aaacgcgacg tctatgcgtt ctcgaagcac gaaggggtgc ctgccgcgga tccgttcgcg
     3901 ggggttggat tcgcaacgtg gctatgcaac gcggggccga gacaggtcta ctctgatggt
     3961 ta
//
LOCUS       DATMED010000441         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1897250, whole genome shotgun sequence.
ACCESSION   DATMED010000441 DATMED010000000
VERSION     DATMED010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897250"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VNP73_11590"
     CDS             <1..1371
                     /locus_tag="VNP73_11590"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWL66608.1"
                     /translation="PLQGRIGVGYSIAYGVEHASAAEPSLTLLDVDRAIGQIEMTVGS
                     GSAGERKRILGDLFAQATASEADFLRRLFIGELRQGALEGVMLEAIAAAAEVPSTVVR
                     RALQLAGDLPHTGAIALSSGESGLREIGLEIFRPVRPMLASTAEDVATAIDGFELASV
                     EWKLDGIRIQIHRLDTEVRIYTRNLNDITHALGGIAAAVRKLPVRRAVLDGEALWFDE
                     SGPASFQETMSRIDTDAPPEGVETFLFDLLHVDGDDLIDLPLKERAARLEAIAPNLRI
                     PSALTSDPDEAQRVLDDALRAGHEGVVVKDAASLYGAGRRGKAWRKVKPVQTYDFVVI
                     GAEWGHGRRQGWLSNLYLAARDPNSGEFVMVGKCFKGLTDELLGWQTQALLERETERT
                     GHAVLVRPELVVEIAIDGVQSSTRYQGGVALRFARVKRYRPDKNPTESDTIDALRNLL
                     QRPSFE"
     gene            1402..1800
                     /locus_tag="VNP73_11595"
     CDS             1402..1800
                     /locus_tag="VNP73_11595"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HWL66609.1"
                     /translation="MDINWLAVLAGTVAHQALGALWYGVIFRKTWLNAMGMNPEEIER
                     EGPGGEMAFGAVASLVSVIALAFIMKWVGDPTVAKGICLGAVTGIGFVAASTFMNGMY
                     EQKKPILSVLFGAYYTLGLIIAGVILGAWR"
     gene            complement(1778..>2027)
                     /locus_tag="VNP73_11600"
     CDS             complement(1778..>2027)
                     /locus_tag="VNP73_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWL66610.1"
                     /translation="NDPGLRRVTYVTSDGRRFRGIDAIARALEHIHLGWALVGWFLLL
                     PGVKQLAQVMADVFGPGVQKVQGASYDPAACVIATPPK"
BASE COUNT          379 a          641 c          649 g          358 t
ORIGIN      
        1 ccgcttcagg gccggatcgg ggtgggctac tcgatcgcgt acggggtcga gcatgcatct
       61 gccgccgagc cctccctcac gctcctcgac gtcgatcgcg cgatcggtca gatcgaaatg
      121 acggtcggga gcggctccgc gggcgaacgc aaacggatcc tgggcgatct cttcgcccaa
      181 gcgactgcgt cggaagcaga cttcctccgc aggctcttca tcggcgagtt gagacaaggc
      241 gccctcgaag gggtgatgct cgaggcgatc gcagctgcgg ccgaggtgcc gagcaccgtc
      301 gtcagacgag ctctgcagct ggccggagat ctcccccaca ccggcgcgat tgctctgtcg
      361 tcgggagaga gcggtttgcg cgagatcggc ctcgagatct tccgccccgt ccgaccgatg
      421 ctggcttcca ccgccgagga tgtggcgacc gcgatcgacg gcttcgaact cgcatcggtc
      481 gagtggaagc tcgacggcat ccgcatccag atccatcgcc tcgacactga ggtccgcatc
      541 tacacgcgca atctcaacga catcacccat gccctcggag gcatcgctgc agcggtgcgc
      601 aagctgccgg ttcggcgagc cgtgctcgac ggcgaggcgt tgtggttcga cgagagcggg
      661 cccgcgtcct tccaggaaac gatgtcccgc atagacaccg acgctccacc cgaaggagtc
      721 gagacgttcc tgttcgacct gttgcacgtc gacggtgacg acctgatcga tcttccgctc
      781 aaggaacggg ccgcgcgcct agaggcgatc gcgccgaacc tcaggatccc ttcggcactc
      841 acgtcagacc cggacgaagc acagcgcgtc ttggacgacg cgttgcgcgc cggacacgaa
      901 ggtgtcgtcg tgaaggacgc ggcttctctt tacggcgcgg gtcggcgcgg aaaggcgtgg
      961 cgcaaggtca aaccggttca aacctacgac ttcgtggtga tcggcgcgga gtggggccat
     1021 gggcgcaggc aaggttggtt atcgaacctg tacttggctg cacgggaccc gaactccggc
     1081 gagttcgtga tggtcggcaa gtgcttcaaa ggcctaaccg acgaactcct tggatggcag
     1141 acccaggctc tattggaacg cgagaccgag cgcacgggtc acgccgtgct cgtgcgcccc
     1201 gagctggtcg tcgagatcgc gatcgatggt gttcaatcct cgacgcgcta ccaaggcggc
     1261 gtcgcacttc gcttcgcgcg agtcaaacgc taccggccgg acaagaaccc caccgaatca
     1321 gacacgatcg atgccttgcg aaacctgctt caacgccctt cctttgagta aggtcctcta
     1381 aacaagaagc tagggggcgt gatggatatc aactggctgg cggttcttgc gggcacggtg
     1441 gcacaccaag ctcttggggc cctctggtac ggcgtcatct tccgaaagac gtggcttaac
     1501 gcgatgggga tgaaccccga ggagatcgaa cgagagggac cggggggcga gatggcgttc
     1561 ggggccgtcg cttccttggt gagcgtcatc gcactggcat tcatcatgaa gtgggtaggc
     1621 gacccgaccg ttgccaaagg gatctgcctt ggagccgtca ccgggatcgg gttcgtcgcg
     1681 gcttcgacgt tcatgaacgg gatgtacgag cagaagaaac cgatcctgag tgtgctcttc
     1741 ggcgcgtact acacgctcgg tctgatcatc gccggcgtca ttttgggggc gtggcgataa
     1801 cacaggcggc tgggtcgtag gacgcgccct gcaccttctg cactccgggg ccgaagacgt
     1861 ctgccatgac ctgggccagc tgcttcactc ccggcaacag caggaaccac cccaccaacg
     1921 cccaccccag gtggatgtgc tccaatgccc gagctatcgc atcgatcccc ctgaaacgac
     1981 gaccatcgga cgtgacgtac gtgacgcgcc tcaaccccgg gtcgttc
//
LOCUS       DATMED010000442         2959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1897486, whole genome shotgun sequence.
ACCESSION   DATMED010000442 DATMED010000000
VERSION     DATMED010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897486"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VNP73_11605"
     CDS             complement(<1..1327)
                     /locus_tag="VNP73_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66611.1"
                     /translation="MTRKILTGTIVAVLTAVWLQGAASSTAAQTGPAVVQACGKGSWI
                     AGTVDICDGELVYRDYVYDDYGADTSDPTAPSTGILSRPAGDERYPEGQENTADIIDL
                     SLEIDGDQLVTEFEFGALYDPETTVAAVAIDTDADASTGGGAWEGLNVSSEGWDELYR
                     FESGDPKTNLISGRVPLPGGTKWRIQAVAAQKDGRVMNVAFRGTNEETVTGAWWDDKQ
                     AAALAGGDISQFGYKIEVSDLRNGTRKVAEVEPGFHQRVYVSKYTLRKGEGMSYDSEF
                     GRHGDSGEVCEQEFNFFGKYQPYGLYVPAQPGPHAMQLALHGCNANHASLVDQPGMQN
                     VMGEQHNRVIVVPLGRGPIGYYSDISERDVLDVMADVEKHYEIDKDKVYSGGYSMGGY
                     GAFRMAMLYPHRFAAMISWVGFTGDCFNGTVRESDRECKSGAIGNVINYV"
     gene            1480..1878
                     /locus_tag="VNP73_11610"
     CDS             1480..1878
                     /locus_tag="VNP73_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL66612.1"
                     /translation="MAIRVLVADDVKEMRVLLRLAMTRHGGFEVVAEAEDGQSAIEMT
                     GRHKPDVVLLDLAMPGMDGLEAIPLIHRALPGVRILVLSGFSSPAIAARAKELCATDY
                     IEKGESPTIILQALERAFRSPSKDPGADRD"
     gene            complement(1875..>2959)
                     /locus_tag="VNP73_11615"
     CDS             complement(1875..>2959)
                     /locus_tag="VNP73_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66613.1"
                     /translation="LLFPYFLYRFAAAFEKPARATEIVAAALAAGVIIWTFFLPEFPQ
                     PGDPQPPQFRAYVFGILIEWVVLSTIMSVKLWRGGAGQPGVARRRMRLMAAAAGIFSL
                     LIVVAGSAGGEQAVGMQVFNQAMALVAALSFYLGFSPPQLVRSVWRRKPLDQMRRGVT
                     ALLSAESDKEVTKGLLPHMSEIVGAQAIAFVDEDERIVDTYGTTKEMDDSIPEILGRE
                     EDIQESCVLLRFPFGSMIVWTSSYAPFFGTEEFETLVYLGTLAHLALERTRATQLKVQ
                     LAEAKFRRMQALQINDNVVQGLAVSKYAFELGDPDKGMKSLSDTLESAKAIITDLLKE
                     VDPDQTLSPGMLVRDRAASVERSESS"
BASE COUNT          542 a          954 c          894 g          569 t
ORIGIN      
        1 tcacgtagtt gatgacgttg ccgatcgcac cggacttgca ttcgcggtcg gactctctca
       61 ccgttccgtt gaagcagtcg ccggtgaagc cgacccatga gatcatcgcg gcgaaccggt
      121 gcggatagag catcgccatt cggaatgcgc cgtaaccacc catcgagtag ccgcccgagt
      181 acaccttgtc tttgtcgatc tcgtagtgct tctctacgtc tgccatcaca tccaggacat
      241 cgcgctccga gatatccgag tagtagccga tcggaccgcg accgagggga acgacgatca
      301 cccggttgtg ctgctcgccc atcacgttct gcatgccggg ttgatcgacg aggcttgcgt
      361 ggttggcgtt gcaaccgtgc agtgcgagtt gcatcgcgtg ggggccgggc tgcgcgggca
      421 catacaaacc gtacggctgg tacttgccga agaagttgaa ctcttgctcg cacacctcac
      481 cggaatcgcc atggcgtccg aactccgagt cgtacgacat cccctcgccc ttgcgaaggg
      541 tgtacttcga gacgtagacc cgctggtgga accccggctc tacctcggcg accttcctag
      601 ttccattccg cagatcgcta acttcgatct tgtagccgaa ctgagagatg tccccaccgg
      661 ccagagctgc cgcctgcttg tcgtcccacc aagctccggt gacggtctct tcgttggtgc
      721 cgcggaaggc tacgttcatc acacggccgt ctttctgggc ggctacggcc tggatgcgcc
      781 acttggttcc tccgggtagc ggaaccctcc ccgagatcag gttcgttttg gggtccccgc
      841 tctcgaatct gtagagctcg tcccagcctt cactcgaaac attcagacct tcccaggccc
      901 ccccaccggt ggatgcatcc gcgtcggtgt cgatcgccac ggccgcgact gtcgtttcgg
      961 ggtcgtaaag agcgccgaac tcgaactccg tgaccagctg atctccgtcg atctcgaggg
     1021 agagatcgat gatgtctgcg gtgttctcct gcccctccgg gtagcgctcg tcgccggcgg
     1081 gtcgagacag gatcccggtc gaaggggccg tggggtcgga ggtgtcggcg ccgtaatcgt
     1141 cgtagacgta gtcgcgatag acgagttctc cgtcacagat gtcgacggtc ccggcgatcc
     1201 aggaaccctt gccgcaagcc tggaccacag ccgggccggt ctgcgcggcc gtcgacgatg
     1261 ccgcaccctg cagccatacg gctgtcaaaa ctgcaacgat cgttcccgtc agtatctttc
     1321 gtgtcatatc cgccgcctcg tctctccccg gctatcagga caagcaaacc gaaccagtac
     1381 taaaggtcga acatttctac tcaaatcacg tattgcctcc gaacctccta cccacccatc
     1441 ctgtgtccag ggggggctga aacaaggaag gggtgccggg tggcaatcag ggttctggtc
     1501 gcggacgacg tcaaagagat gcgagttctg ctccgtctgg ccatgactcg ccacgggggc
     1561 ttcgaggtag ttgccgaggc cgaggacggc cagtcggcca tcgagatgac cggacgccac
     1621 aagcccgatg tggtgctgct cgacctggcc atgccgggca tggacgggtt ggaggcgatc
     1681 ccgttgatcc accgagcact cccaggggtt cggatcctcg tgctttcggg gttctcctcg
     1741 ccggcgatcg ctgcccgggc caaggaactc tgcgcgacgg actacatcga gaagggcgag
     1801 tctccgacga tcatcctgca ggccctcgaa cgggcgttcc gatcaccgtc caaggacccg
     1861 ggcgccgacc gcgactagct gctctcgctt ctttcgaccg aagccgcccg gtcccgaacg
     1921 agcatcccgg gtgacagggt ctgatcggga tccacctctt tgagcaggtc ggtgatgatg
     1981 gccttggcgg actccagcgt gtcggacagc gatttcatac ccttgtcggg atctcccagc
     2041 tcgaacgcgt acttcgacac cgccaagcct tggaccacgt tgtcgttgat ctgaagcgcc
     2101 tgcatcctgc ggaacttcgc ctcggccagc tgaaccttca gttgggttgc ccgggttcgt
     2161 tccaaggcca agtgagcgag ggtcccgaga tagaccaggg tctcgaactc ctccgttccg
     2221 aagaagggcg cgtacgacga cgtccacacg atcatggacc cgaacgggaa gcgcagaagt
     2281 acgcaggact cctggatgtc ctcctcacgc cccaggatct ctgggatcga gtcatccatc
     2341 tctttcgtgg tgccatacgt gtccacgatc cgctcgtctt catccacgaa tgcgatcgct
     2401 tgggctccca cgatctcact catgtggggg aggagaccct tggtcacttc cttgtcggat
     2461 tccgcgctca gaagagcggt gacgccgcgg cgcatctggt cgagtggctt gcggcgccag
     2521 acggatcgca ccaactgtgg cgggctgaaa ccgaggtaga acgacagggc agctaccagc
     2581 gccatggctt ggttgaagac ctgcatcccg acggcttgct cgccgccggc ggacccggcg
     2641 acgacgatga gcaaactgaa gatccctgcc gccgccgcca tcagtcgcat gcgccggcga
     2701 gcgacgccgg gttgcccggc ccccccacgc cacagcttca ccgacatgat cgtcgacagc
     2761 acgacccact cgatcaggat gccgaagacg taggcacgga actgcggggg ctgtggatcg
     2821 cccggttggg ggaactcggg caggaagaag gtccagatga tgacgcccgc cgcaagagct
     2881 gctgccacga tctcggttgc gcgcgccggt ttctcgaagg ccgctgcgaa ccggtacagg
     2941 aagtaaggaa acaagagca
//
LOCUS       DATMED010000443         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2678508, whole genome shotgun sequence.
ACCESSION   DATMED010000443 DATMED010000000
VERSION     DATMED010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678508"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VNP73_11620"
     CDS             complement(<1..526)
                     /locus_tag="VNP73_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66614.1"
                     /translation="MPLLDFSDVRAALDNGKNFGGVTNTGWHADAVYERFSDQEYRRR
                     YEATYAVMDSLGLDALIVGGGPSHWSSGGGMLWLTGHFEWHALACYVLVPRHGDPTLV
                     YSMGGSHIETVRSTSWVTDVRPSRMGAFGDVLVEAAKEAGLENARIGHPPIDARFKDY
                     MPVNQHEAITEGLPD"
     gene            827..2488
                     /locus_tag="VNP73_11625"
     CDS             827..2488
                     /locus_tag="VNP73_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWL66615.1"
                     /translation="MSALFVVLLLVAACNPSADEADTAGAGGEVQNPDFLVITTDDEP
                     ETLDPAAAADNGLGRAAIMYGYDRLVDVGAETIELSPMLATEVPTLENGLISEDGLTY
                     TFPLREDVKFHDGSDLTAEDVVYSWERVQTMNLPEGQAFLFENIAEMKAVDDYTFEVT
                     LKEVDASFLYGTVGAMPASIVNPDVVEENGGVVDNEPNEFMDANMAGTGVYKFESWQR
                     GERLNLEINEDYWGEKAKLDLRWLNVQDPNVSTLGLRAGDYDMIEGVPSIIGDIQGAP
                     GVEVKTDTPGLQLLQIGFNLRFREEDLPDGDTVPADFFHDVRVRQAFNYAFDYQAMIE
                     GPLFGAATRGSYVLPQGLYGYDPEAPIFDYDLERAEELFKEAGWWEEGFTVSVIAEED
                     SGFTDQALIMKDGVESLNPKFRVNVQALPEARFDELMAQDPIPVAMWSWTSPEFRDPH
                     AYMLDSAHTEGRWGTIAGFENGYEDPDAIDEMITAAQRELDPDARAELYSELQQVLFD
                     EAMWIFTAQESAVLAYRDWLTGVTVNPMWPRPALRYSLYGKGGES"
     gene            2531..3553
                     /locus_tag="VNP73_11630"
     CDS             2531..3553
                     /locus_tag="VNP73_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWL66616.1"
                     /translation="MQIRDYVLRRVMLLPFLVVATTLIVFILMRAGGSPLGVYLQPGM
                     SQEQIARLEDRYNLNEPLPLQYVSWLGGVLRGELGYSGVSSAPVTEVFPQKLTATAEL
                     AVAAGAIAVVLGISMGTFAARRRDKLADHLTRVLAISGASMPTFWFGIVMLIVFWAGF
                     GLFPIGRSDPDVWASIAHPTGLYTVDAILAGNFNALLDALWHLVLPAAVLGYSTTAII
                     ARMMRSSLLEELGQDYVDAARAKGLPERLVVRRHARRNALMPTITVIGLTFGILIEGT
                     VVVEIIFVWPGLGKWLSDAVLEGDQATTLAYVLFISIVFLFLNLIVDLAYAYLDPRVV
                     LGDLED"
     gene            3557..>3914
                     /locus_tag="VNP73_11635"
     CDS             3557..>3914
                     /locus_tag="VNP73_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66617.1"
                     /translation="MASEIGFSGRVGLFRESGFSARAGRIGAILWEAFRKPTTAIGGV
                     IIVVLLGVAVFAPLIMEPNQPDAYQLPRDWRLTNAPPGTDGHLLGTTAEGGDVLYGIV
                     WGARSSVRLSVTVVAVA"
BASE COUNT          707 a         1176 c         1288 g          743 t
ORIGIN      
        1 tatcggggag accttccgtg atggcctcgt gctgattgac cggcatgtag tccttgaagc
       61 gagcgtcaat cggcgggtgg ccgatgcgcg cgttctctag cccggcctcc ttcgccgctt
      121 cgacgagcac gtcgccgaac gcgcccattc gcgacgggcg gacgtcggtg acccaagagg
      181 tgctgcgcac ggtttcgata tgacttccgc ccatcgagta gacgagcgtt ggatccccgt
      241 gtcgcgggac gaggacgtag caggcaaggg cgtgccactc gaagtggccg gtaagccaga
      301 gcattcctcc gcccgagctc cagtggctgg gcccaccccc cacgatcaac gcgtcgagcc
      361 cgagagagtc catcaccgcg tacgtcgcct cgtacctgcg tcggtactcc tggtcggaga
      421 aacgctcgta gacggcgtct gcgtgccagc ccgtgttggt cacgcccccg aagttcttgc
      481 cgttgtcgag ggctgcacga acgtcggaga aatcgagcag tggcatgcgg cctcccgcgc
      541 tagaagggta aggagcggtc tttcgctctc ttgatggtgg cgccgcgcaa ccccactgca
      601 aacgtttgcc gagccagggt aactaggggc cctccggctg tcaagaaacc cgtcccgagg
      661 tgacaacaca agtgtccagg attcgaaggc atccgctcca aacacgttga cacccgttcg
      721 cggcgccgcc tatactgcaa tcgtttgcgg cggggtttac ctcaattacc accagggggg
      781 tgggatggac gcaatctctg gaagtcggcc tcttaggcga tggagggtga gcgctctctt
      841 cgtcgttctg ctactggtag cggcatgcaa tccctccgcg gacgaggccg atacggccgg
      901 cgcgggaggc gaggttcaga accccgactt cctcgtcatc actaccgacg acgagccgga
      961 aaccctggac ccggccgccg cggcggacaa cgggctggga agagcggcga tcatgtacgg
     1021 ctacgaccgt ttggtcgacg tcggagcgga gaccatcgaa ctgagcccga tgctcgcgac
     1081 cgaagtaccg acgctcgaga acgggctcat ctcggaagat ggactcacct acaccttccc
     1141 cttgcgcgag gacgttaagt ttcacgacgg cagcgatctg accgccgagg acgtcgtgta
     1201 ctcctgggag cgcgtgcaga ccatgaatct ccccgagggg caggccttcc tgttcgagaa
     1261 catcgccgag atgaaggcgg tggacgacta caccttcgag gtcacgctga aggaggtgga
     1321 cgcgtccttc ctatacggga ccgttggggc gatgcccgca agcattgtta accccgacgt
     1381 cgtcgaggag aacggcggcg tggtggacaa cgaacccaac gagttcatgg acgcgaacat
     1441 ggccggaacc ggcgtgtaca agtttgagag ctggcaacgg ggcgagcgac tcaacctcga
     1501 gatcaacgag gactactggg gtgaaaaggc gaagctcgac ctacgctggc tgaacgttca
     1561 ggaccccaat gtctcgaccc ttggtctgag ggcaggcgac tacgacatga tcgagggcgt
     1621 gccgtcaata atcggcgaca ttcagggggc gccgggcgta gaagtgaaga ccgacactcc
     1681 cggtctgcag ctactgcaaa tcggtttcaa cctgcgtttc agggaggagg acctccccga
     1741 tggcgacacc gtgccggcgg atttcttcca cgatgtgcgc gtccggcaag ccttcaacta
     1801 cgcgttcgac tatcaggcga tgatcgaggg gccgctgttc ggagcggcga cgcgaggcag
     1861 ttacgtcctc ccgcagggtc tctatgggta cgacccggag gcgccgatct tcgactatga
     1921 cctcgagcgc gccgaggagc tcttcaagga ggcgggctgg tgggaggagg gcttcacggt
     1981 gtcggtcatt gccgaggagg acagtggctt caccgatcag gcgctcatca tgaaggacgg
     2041 agtcgagagc ctgaacccca agttccgcgt caacgtgcag gccttgccag aggccaggtt
     2101 cgacgagctg atggcgcagg acccgattcc agtcgcgatg tggtcgtgga ccagccccga
     2161 gttccgcgat ccccatgcct acatgctgga ctcggcacat accgagggac gatggggaac
     2221 catcgcggga ttcgagaacg gctacgaaga cccggacgcg atcgacgaga tgatcaccgc
     2281 ggcacagcgt gaactcgatc ccgacgccag agccgagctc tacagcgagc ttcagcaggt
     2341 gttgttcgac gaggcgatgt ggatctttac ggcgcaggaa tccgcggttc tcgcataccg
     2401 ggactggctg accggggtta cggtgaaccc aatgtggccg aggccggctc ttcgctattc
     2461 gctctacgga aagggcggtg agagctaggc cgtgccccgg tcccctcgag ctcgattctg
     2521 aggaccttga atgcagattc gtgattacgt cctgcgacgg gtgatgctgc ttcccttcct
     2581 ggtggtggcg acgactctga tcgtcttcat tctgatgaga gcgggaggct cgccgctggg
     2641 cgtttacctg cagccgggga tgtcgcagga gcagatcgcc cggcttgagg atcgctacaa
     2701 cctgaacgaa ccgctaccgc tccagtacgt atcgtggctg gggggagtgt tgcgggggga
     2761 gctcgggtac tcgggagtgt cgtcggcgcc cgtgactgag gtctttccac agaagctgac
     2821 agcaaccgcc gagctcgcgg tcgcggccgg ggccatcgca gttgtgctcg gcatttcgat
     2881 gggaacgttt gcagcgagga gacgcgacaa gctcgcggac catctgacaa gggtgctcgc
     2941 catcagcggg gccagcatgc ccacgttttg gttcggcatc gtcatgctga tcgtgttctg
     3001 ggccggcttc ggcctgttcc ccattggccg gtcggatccg gacgtttggg cctccatcgc
     3061 ccatcccacc ggtctctata ccgtcgatgc gattctggcg ggcaatttca atgcgctgct
     3121 cgacgcgctc tggcatctgg ttcttcccgc ggccgttttg ggctacagca ccacggccat
     3181 catcgccagg atgatgcgct cctcgctgct cgaagagctc gggcaggatt acgtcgacgc
     3241 ggcgcgcgcg aaaggtctcc ccgagcgcct ggtcgtccgc cgtcacgcgc gtcgcaacgc
     3301 gctgatgccc acgatcacgg tgatcggatt gactttcggg atactcatcg aaggaaccgt
     3361 ggtagtggag atcatcttcg tgtggccggg gttggggaag tggctctccg atgcggtgtt
     3421 ggagggggat caggcgacga cgcttgcgta cgtactcttc ataagcatcg tgttcctgtt
     3481 tctgaacctg atagtggact tggcctacgc ctacctggac ccacgggtgg tcctggggga
     3541 cctggaggac tgaggcatgg catctgagat cggattctcg ggtcgggttg ggctcttcag
     3601 ggagtccggg ttctcggctc gcgcgggccg aatcggcgcc atcctttggg aggcgttccg
     3661 caagccgacc accgcgatcg gcggagtgat catcgtcgtg cttctcggag tggcggtgtt
     3721 cgcaccgctc atcatggagc ccaaccagcc cgacgcttac cagttgccgc gcgactggag
     3781 gcttaccaac gctccccccg gcaccgatgg tcaccttctc ggtaccaccg ccgaaggagg
     3841 agacgttctc tacgggatcg tgtggggagc gcgctcctcg gtcaggctct ccgtcactgt
     3901 ggttgcggta gcgc
//
LOCUS       DATMED010000444         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_2678553, whole genome shotgun sequence.
ACCESSION   DATMED010000444 DATMED010000000
VERSION     DATMED010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678553"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VNP73_11640"
     CDS             complement(<1..372)
                     /locus_tag="VNP73_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWL66618.1"
                     /translation="MIRVALAQLDLPVGDIDGNAARLEEVIEQIEDSGAQIVAFPELA
                     ITGYPPEDLVLKGSFITANLRAMERVAAASRDALVVFGFVEPAGDRLYNSAAVCRGGE
                     ILAVYRKHLLPNYGVFDEQRYF"
     gene            451..1797
                     /locus_tag="VNP73_11645"
     CDS             451..1797
                     /locus_tag="VNP73_11645"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWL66619.1"
                     /translation="MERQQDYVLRTVEERGIRLVWLWFTDVLGFLKSFAVTSEELEQA
                     FAEGIGFDGSAIEGFARVQESDMLARPDAGTFQIIPPRQEQTEAGVARMFCDIYTPDG
                     RPFWGDPRYVLRRNLERASERGFTFYVHPEMEFFLYKDSHDPTPIDAGGYFDLTPLDI
                     TQDFRRETINVLEQMGIPVEFSHHEVAPSQHEIDLRHADALMMADSVMTFRLVVKETA
                     AARDMYATFMPKPRTDLPGSGMHTHMSLFEGDENAFHDSSDEYHLSKVAKAFIAGMLV
                     HSREITAVTNQWVNSYKRLITGRGYPIEEAPAYVCWGRHNRSALIRVPMYKPRKEQST
                     RIELRSPDPACNPYLAFSVMLAAGLKGIDEGYELPGEATDNIHEMTESERRAKGIAQL
                     PDDLYEALGEMERSELVAETLGEQVFEYLLRNKRAEWNDYKGYVSPYETERYLPLL"
     gene            complement(1798..>2361)
                     /locus_tag="VNP73_11650"
     CDS             complement(1798..>2361)
                     /locus_tag="VNP73_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWL66620.1"
                     /translation="PTPDPSPSGSSNPSGPSACSDGIDNDEDDATDGDDEQCEGPDDD
                     DESGPAPQCGTEGVVCGTSGDDNIQGTAADETIIGGGGDDTCAGGGGDDVLFCGAGND
                     VLRGGSGNDFVRSIWGRDRLFGGIGFDRIHGGFGNDTLVGGKGPDNLIGEAGFDTIDG
                     GGGNDRCVGGAGDEKTTSCEVQPKHDF"
BASE COUNT          432 a          774 c          722 g          433 t
ORIGIN      
        1 gaagtagcgc tgctcgtcga agactccgta gttcggcaga aggtgcttgc gatagacggc
       61 gaggatctcg ccgcctcggc agacggcggc cgagttgtag aggcggtctc ccgcgggctc
      121 cacgaatccg aagacgacca acgcatcccg cgacgcggct gcaacacgtt ccatggcccg
      181 aagattcgcg gtgatgaacg atcccttcag aacgaggtcc tcgggcgggt acccggtgat
      241 cgcgagctct ggaaatgcca cgatctgggc acccgagtcc tcgatctgct cgatcacctc
      301 ttccaggcgc gccgcgttgc cgtcgatgtc gccgacggga agatccaact gtgccagcgc
      361 gaccctgatc accccacaag cctagtgcgc cgatcgcgaa agggatccgt gtaaccagtt
      421 cgaaactttc acggcgtaat gtcctcgacg atggaacgac aacaggacta cgtgctcagg
      481 acggtcgagg agcgggggat ccgcctcgtc tggctgtggt tcaccgacgt gctcgggttc
      541 ctcaaatcct tcgcagtcac gtctgaagag ctcgagcagg ccttcgccga ggggatcggc
      601 ttcgatggct ctgcgatcga gggcttcgcc cgggtgcagg agtcggacat gctcgcccgc
      661 cccgatgcgg gcaccttcca gatcatcccc ccacggcaag agcagacgga agccggcgtc
      721 gcccggatgt tctgcgacat ctacacgccc gacggccggc cgttctgggg cgacccccgg
      781 tacgtgcttc ggcggaacct agaaagagcg tcggaacgag gcttcacgtt ctacgtgcat
      841 ccggagatgg agttcttcct ctacaaggac tcgcacgacc cgaccccgat cgatgccggc
      901 ggctatttcg accttacgcc gctcgacatc acacaggact tccgtcgcga gaccatcaac
      961 gtgctcgagc agatggggat cccggtcgag ttctcccacc acgaggtcgc gccttcacaa
     1021 cacgagatcg atctgcgtca cgcggatgcg ctgatgatgg ccgactcggt catgacgttt
     1081 cgtttggtcg ttaaggaaac cgccgccgca cgcgacatgt acgcgacgtt catgcctaag
     1141 ccacgcaccg acctacccgg gtccgggatg catacgcaca tgtcgttatt cgaaggagac
     1201 gagaacgcgt tccacgactc gtcggacgag tatcaccttt ccaaggttgc gaaggcgttc
     1261 atcgccggca tgctcgtgca ctcgcgggag atcactgcgg tcacgaacca gtgggtgaac
     1321 tcctataaga gactcatcac cgggcgcggc tatccgatcg aagaggcccc cgcttacgtt
     1381 tgctggggcc gtcacaaccg ttcggcactc atccgcgttc cgatgtacaa gccgcgcaaa
     1441 gagcaatcga cgcgcatcga gcttcgctcg cccgatcccg cgtgcaatcc ttatctggcg
     1501 ttctctgtga tgctcgcggc cggcctcaag gggatcgacg agggttacga gcttcccggt
     1561 gaggcgaccg acaacatcca cgagatgacc gagagcgagc gccgcgccaa gggcatcgcg
     1621 cagctgccgg acgatctcta cgaagcactg ggcgagatgg agaggtccga gttggtggcc
     1681 gagacgctcg gcgagcaggt cttcgaatac ctattgcgga acaagcgggc cgagtggaac
     1741 gactacaagg ggtacgtgag tccctacgag acggagcgct acctgcccct tctctgatca
     1801 gaagtcgtgt ttgggctgga cctcgcagct cgtcgtcttc tcgtcgccgg ccccaccgac
     1861 gcaccgatcg ttgccgccgc caccatcgat cgtgtcgaat cccgcttcgc cgatgaggtt
     1921 gtcgggccct ttaccgccga caagcgtgtc gttgccgaag ccaccatgga tgcgatcgaa
     1981 accgatcccg ccgaagaggc gatcccgacc ccagatgctt cggacgaagt cgttgccgct
     2041 accgccgcgg agaacgtcgt tgccggcgcc gcagaagagc acgtcgtcgc cgccaccccc
     2101 ggcacaggtg tcgtcaccgc cgcctccgat gatcgtctcg tcggccgccg ttccctggat
     2161 gttgtcgtcg ccgctggtcc cgcacacgac gccctcggtt ccacactgcg gggccggccc
     2221 actttcgtcg tcgtcgtccg gcccttcaca ctgttcgtcg tcgccgtcgg tggcgtcgtc
     2281 ttcgtcgtta tcgatcccat cggagcatgc ggagggaccg ctcgggttcg agcttccact
     2341 gggggatgga tcgggtgtcg g
//
LOCUS       DATMED010000445         8439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1115879, whole genome shotgun sequence.
ACCESSION   DATMED010000445 DATMED010000000
VERSION     DATMED010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115879"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..589
                     /locus_tag="VNP73_11655"
     CDS             245..589
                     /locus_tag="VNP73_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66621.1"
                     /translation="MRAPGQRADGTYSASASKTIATTTKKAYDAFASDDIRSNWLGDH
                     PWELGTVRVGKSLTARWEDGTRLYVSFEPKDKGKVQVALAHERIPEASRADQMKLFWR
                     EHLGFLKKVLEG"
     gene            636..1781
                     /locus_tag="VNP73_11660"
     CDS             636..1781
                     /locus_tag="VNP73_11660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWL66622.1"
                     /translation="MGEAIGERREVTASHPTMLPPADAGGGRDDLIDLLRSLATTTVV
                     LWHWVFTILVWRVDGPHADNPIGYVSGLWSITWVLQVMPLFFVAGGYVHSKAWARHRG
                     KKGAWPHFVKRRAVQLGVPALVLIAVVAGSVTAAGYLHPGPDPWVTRSILLVLSPLWF
                     LIVYMALVATVPIWDWLDRRWGYLAPIGLAVATMGVDLLRFRFGHPEAAWFNMVFVWG
                     AAHQVGWSWERLRVAPYRFGHALMLIGFAGLAGLTNMGLYPRSMVGTTSAADRFSNMG
                     PPTLPIVALLIFQVGLVVAHRDRILSWAENPRVQRFVEWISANAMPLFLWHAVGFAIF
                     YGLMRLFAFVPEEPNLIWWVTRPLWVLGPALLTVPLLAATKRVRPAA"
     gene            1794..2498
                     /locus_tag="VNP73_11665"
     CDS             1794..2498
                     /locus_tag="VNP73_11665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWL66623.1"
                     /translation="MWFALAMSGVLVLYNNVANRWPLFQGAAYVPLNLTVTAAVTLLC
                     AVSLGLSRSELGLTGDRLDVAVSVVALNIFVFGVFTIARSRHAHRIADKRVADLRGGK
                     LLYQVLVRVPLGTAVTEELLFRGVLFALWSEAGQSVPASALWASIAFGLWHITPTIVG
                     VRMNDAHASSRKVGTAVVGAIILTTLAGLGLTWLRLWTGGLVAPIVLHAGVNSIGTLA
                     AVEGARACGKPPRPFR"
     gene            complement(2461..3099)
                     /gene="lexA"
                     /locus_tag="VNP73_11670"
     CDS             complement(2461..3099)
                     /gene="lexA"
                     /locus_tag="VNP73_11670"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWL66624.1"
                     /translation="MVEGLTDRQRQTLEFIAETVAERGYPPSVREACEALGLASSSTV
                     HSHLQALQRKGYIKIDPTKPRAIEILFDPETGLAAERRPARSVPLLGRIAAGTPILAD
                     ENVEEIYPMPSDLVGDGNVFMLEVHGDSMIDAGIMDGDFVVINQQPTAHSGEIVAALI
                     PSDHGDAEATVKTIRHRGSEIVFEPANPRLQPFEAPQGTEIIGKVVAVFRKL"
     gene            3341..3721
                     /locus_tag="VNP73_11675"
     CDS             3341..3721
                     /locus_tag="VNP73_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWL66625.1"
                     /translation="MAVRSEELYLQRERPAGRSRRTTARVYKFPPVRARRAAVHRRRL
                     ALGTVAVLVVVAGLFATGPSGVAPAASQRVPRSVVLHEGDTVWEIADRYAPENMDVRT
                     YIDAIVRLNGLEGLPPTGTKLRLP"
     gene            3948..6722
                     /locus_tag="VNP73_11680"
     CDS             3948..6722
                     /locus_tag="VNP73_11680"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWL66626.1"
                     /translation="MTPGKTAAATTTTRATGDELVINRHFTTEGIHPYDEIEWEHRDA
                     VIQNYKTGEVAFEQKAVEVPKSWSQNATNIAAQKYFRGSQGTPQRETSVKQMIDRVVN
                     KYHEEGLARGYFAGPTEAQAFKDELTHLLVNQKAAFNSPVWFNVGWREQPQVSACFIL
                     SVEDEMSSILNWYKEEGLIFKHGSGAGLNLSKLRSSKERLKSGGTASGPVSFMRGADA
                     SAGTIKSGGATRRAAKMVVLNVDHPDVKDFIWCKALEENKARALREAGFDMDLDGKDS
                     HSIQYQNANNSVRVTDDFMSAVMDDAEWQFRAVTDGSPVETVKAKELMREIAQAAWEC
                     ADPGFQYDTTINEWHTCPNTDRINASNPCSEYMHVDNSSCNLASLNLMKFVDSDNTFD
                     IDGYKHAIEVMFLAQEISVGFASYPTDKIRENTMKMRQLGQGYANVGALLMAQGLPYD
                     SEEGRSWAASLMAIMTGHCYATSAKIAERVGVFEEYPKNSDPMLAVMRKHREEAYKIA
                     EDKAPPELAQAARAAWDDAVRLGEQHGYRNAQATVLAPTGTIGLLMDCDTTGIEPDLA
                     LKKMKKLVGGGTMSIVNQTVPRALRKLGYNETQVADIVRFIDENSHVVDAPHLRNEHL
                     PVFDTAMGERSIHYMGHIRMMAAVQPFISGAISKTVNLPEDVTVEDVEEAYMAGWRMG
                     LKAIAIYRDNCKVGQPLNIAKKDDKEAKVEARTPERRRLPKKRNSKTIKFRVADTEGY
                     LNVGEYDDGSVGEIFLKVAKQGSTLSGIMDAFAISVSMGLQYGVPLAAYVKQFKDTRF
                     EPSGMTDDPDFRLATSIIDYVFRVLALEYMSDEERHALNIRTAGERKAEIETKLDPNA
                     TGDTPNGNGNGNGNGNGNGAHDEKVIVLGPVASAISDSLPFCGQCGVQMNPAGSCFAC
                     PSCGSTSGCS"
     gene            6709..6924
                     /locus_tag="VNP73_11685"
     CDS             6709..6924
                     /locus_tag="VNP73_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66627.1"
                     /translation="MAAAKKPPLCSECGAVLTTGPARCPLCGTEPSQSKPAAKKSQVY
                     DRDKYQAAIRDLRKKLKEIRDEGAEAV"
     gene            6943..7677
                     /locus_tag="VNP73_11690"
     CDS             6943..7677
                     /locus_tag="VNP73_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66628.1"
                     /translation="MARKPVTIGLVLALLSIPLATNAAPYGKPYFYTFSSRYTEGETI
                     TFKLRHDQHTKVMRMGETWDVVDAEGSVLAQYYWEDDQRWLPPHQYRLWEWDQHQACS
                     GACQNVWEGELVPPGRYRIVTTVDDQEVAKWFSVGAFFHLGFDGRPATDFVVFSNNAD
                     VVGQLRAELDHSQEERQIVAGIVRTRKPGYNTDWSFVLDPPSIFLGEVFVEVCDGAPR
                     YIERHRNEWRGQQWCPWSSYVAAEGL"
     gene            7678..8145
                     /gene="nrdR"
                     /locus_tag="VNP73_11695"
     CDS             7678..8145
                     /gene="nrdR"
                     /locus_tag="VNP73_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWL66629.1"
                     /translation="MLCPYCSADEDKVVDSRLAEEGRAIRRRRECITCGRRYTTFERA
                     EEVPLLVAKRNGEEEPFERTKIIDGLRSACKNRPVSDAEIEALTDDVEEAMRADPRRP
                     VPSAEIGREVLERLRELDDVAYVRFASVYKDFQELTDFEQELGILQKKTAPKA"
     gene            8142..>8439
                     /gene="dut"
                     /locus_tag="VNP73_11700"
     CDS             8142..>8439
                     /gene="dut"
                     /locus_tag="VNP73_11700"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWL66630.1"
                     /translation="MKLLVTRLDPDLPLPRYAHEGDAGLDLLAAEDVALLPGARAAVP
                     TGIAVAIPDGYVGLVHARSGRSLKEGLALANAPGVVDSGYRGEIKVICVNLDPSS"
BASE COUNT         1564 a         2603 c         2777 g         1495 t
ORIGIN      
        1 gttcacgaca tcggcgaagt cgtcaaagag gagtacaccg aggacggcac acgtttactc
       61 gccagggtcc ctcgggagag cctgacgaat ttctccgagt tcgtgaccta gccgactctg
      121 ttcatcaaaa gttgccgtgt cgtgggttct tgttgagcag agtggtctgt gtcacgccgc
      181 ggaaagagga gcatgcgatg accaagcaag cagccttcaa ggagcgcatc cgcgcaagag
      241 agggatgcgt gccccggggc agcgtgcaga cggcacctac agcgcctctg ccagcaagac
      301 cattgctacg accacgaaga aggcctacga cgcgttcgcg agcgacgaca tccgtagtaa
      361 ttggctcggg gaccatccgt gggagctagg gaccgttcgg gtgggcaagt cacttaccgc
      421 ccggtgggaa gacggcacgc gcttgtacgt cagcttcgag cccaaggaca agggcaaagt
      481 ccaggtcgcg ctggcccacg agcggatccc cgaggcgagc cgcgcagacc agatgaagct
      541 cttctggcgc gagcacctcg gcttcctgaa gaaggtgctg gagggctaac aacgaatacg
      601 ccgatccaga ggccaccggc gcgatgctga acggcatggg ggaggcaatc ggcgagcggc
      661 gtgaggtgac cgcgtcgcat ccaacgatgc tcccccctgc cgacgccggc ggggggcgcg
      721 acgatctcat cgatctgctg cgttcgctcg cgacgaccac ggtcgtccta tggcactggg
      781 tcttcacgat cctcgtgtgg cgcgtcgacg ggccacatgc agacaatccg atcggttacg
      841 tgtcggggtt gtggagcatt acgtgggtgc tccaggtcat gccgttgttc ttcgtcgccg
      901 gcggttacgt ccattcgaag gcgtgggctc gccatcgtgg caagaaaggc gcgtggccgc
      961 acttcgtcaa gcgacgagcg gtgcagctcg gcgtaccggc gctggtgctg atagccgtgg
     1021 ttgcagggtc ggtcaccgca gccggatacc tgcacccggg ccccgacccc tgggtgacgc
     1081 gttcgatcct gctcgtgcta tcaccgctgt ggttcttgat cgtctacatg gcactcgtag
     1141 ccaccgttcc gatctgggac tggctcgatc gacgttgggg ctacttggct cccatcggtc
     1201 tggcggtcgc caccatgggc gtggatctgc tgcggttccg tttcgggcat ccggaagcag
     1261 cgtggttcaa catggtgttc gtgtggggag cggcccatca ggtgggctgg tcgtgggagc
     1321 ggctccgcgt tgcgccctat cgattcgggc acgcactcat gttgatcggt ttcgcggggc
     1381 tggcggggct caccaacatg ggtttgtacc cccgctcgat ggtgggaact acctcggctg
     1441 cggatcggtt ctcgaacatg ggaccaccga cgttgcccat cgtcgcgttg ttgatcttcc
     1501 aggtggggct agtagttgcg caccgcgatc ggatcctcag ctgggcggag aatcccaggg
     1561 ttcagcggtt cgtcgagtgg atctccgcca atgccatgcc gctgttcctg tggcacgccg
     1621 tcggttttgc gatcttctac ggtctgatgc gcctctttgc attcgtgccg gaggagccca
     1681 atctcatctg gtgggtcaca cggccgctgt gggtgctcgg ccctgcgcta ctgacggtgc
     1741 cactgctggc ggcaaccaaa cgcgtgcgcc cggctgcctg accctccgca accatgtggt
     1801 tcgcgttggc gatgtccggt gtcctggttc tctacaacaa cgtcgctaat cgctggcccc
     1861 tgtttcaggg ggccgcttac gttccactca acctgaccgt caccgcggca gtcactctgt
     1921 tgtgcgcggt gagcctgggg ctgtcgcgct ctgagctcgg tttgacgggt gaccgtctgg
     1981 atgtggctgt gtccgtagtg gcactcaaca tcttcgtgtt cggcgtgttc accatcgcgc
     2041 gctctcgtca cgcacaccgg atcgccgaca agcgcgtcgc cgatctgcgg ggagggaaac
     2101 tcctctacca ggtgctcgtg cgcgtgccct tgggaacggc ggtgacggag gagctcttgt
     2161 tccgcggcgt gctgtttgcg ttgtggagtg aggccgggca atcggtcccg gcttcagcgc
     2221 tgtgggcatc catcgcattc gggttgtggc acataacgcc aacgatcgtc ggcgtccgca
     2281 tgaacgacgc ccatgcctcc agtcgaaagg tggggaccgc cgtggtcggg gcaatcatcc
     2341 tcacgaccct ggccggctta ggcctcactt ggctacgtct gtggaccggg ggactggtcg
     2401 ctccgatcgt ccttcatgct ggggtcaact cgatcggaac cctcgctgcg gtggaggggg
     2461 ctagagcttg cggaaaaccg ccacgacctt tccgatgatc tcggtccctt gcggggcctc
     2521 gaacggctgc agccgcgggt tcgccggttc gaacacgatc tctgatccgc ggtgccggat
     2581 cgtcttgacc gttgcctcgg cgtcgccgtg atcgctcggg atcaacgcgg caacgatctc
     2641 accggaatga gccgtcggct gctgattgat cacgacgaag tcgccgtcca tgatcccggc
     2701 gtcgatcatc gaatccccat ggacctcgag catgaagacg ttgccgtccc cgacgaggtc
     2761 cgacggcatc gggtagatct cctccacgtt ctcgtcggcc aggatcgggg taccggcggc
     2821 gatccggccg agcaggggga ccgagcgtgc cggccggcgt tcggcagcaa gccccgtctc
     2881 ggggtcgaac aggatctcga tcgcccgcgg cttcgtcggg tcgatcttga tgtagccctt
     2941 ccgctggagc gcctgaaggt gggagtgaac ggtcgaggat gaggccaggc ccagtgcctc
     3001 gcaggcctcc cggaccgatg ggggatagcc ccgctcggca accgtctcgg cgatgaattc
     3061 gagggtctgt cgctgcctgt cggtgagccc ttcgaccatg acggcctccg tttcactttt
     3121 cgcgctctta tccgaacgtc tgttcgctag agggtaggcc ccaggggggc caaaagcaag
     3181 aacatctgtt cgattgagcc cttgcacacg aacatacgtt cccctatagt agcgaacgta
     3241 cgttcgggcg ggatggcccc ttgcgggcgg atggccccga aaagtgtcgc accccctctc
     3301 tagcgtgggg gcatgaccca gaccagacag gaggcgacca gtggcagttc gcagcgaaga
     3361 gctctatctc cagcgggagc gaccggctgg gcggtcgcgc aggactacgg cgcgtgtcta
     3421 caagttccct cccgtcaggg cccggagggc agcggtccac cgcaggcgac tggctctagg
     3481 gaccgtggcc gtactggtgg tcgtggccgg gctgttcgcg accggcccct ccggggtcgc
     3541 tccggcggcg tcgcagagag ttccccgctc ggtggtcctc cacgaggggg acacggtctg
     3601 ggagatcgcc gatcgctacg ccccagaaaa catggacgtc cgcacctaca tcgatgcgat
     3661 cgtccggctg aacggcctgg aagggcttcc cccgacgggc accaagctcc ggcttcctta
     3721 gccgccaccg gcttctccac cggcaactca caggctgtgc tccttgttgt tcggggccct
     3781 cggggaggag cgtgagatcg agtgaacggg atacgcgcca ttggcttgac ctttgagtta
     3841 cagaggcgta atttcgtgac cggaattaac aagatgttgg gtctgggggg accaaaaccc
     3901 caacatctgg tgttgaaata ggtgtatcta cgggtgaagg gggcatcgtg acgccaggca
     3961 agaccgcagc cgcgacgacg acgacgaggg cgaccggcga cgagctggtc atcaaccgcc
     4021 atttcacgac ggaggggatc cacccctacg acgagatcga gtgggagcac cgcgatgcgg
     4081 tcatccagaa ctacaagacc ggcgaggtcg cgttcgagca gaaggcggtc gaggtcccga
     4141 agtcgtggtc ccagaacgcc acgaacatcg cggcccagaa gtacttccgc ggctcgcagg
     4201 gaacccctca gagggagact tcggtcaagc agatgatcga ccgggtcgtc aacaagtacc
     4261 acgaggaagg actggcccgg ggttacttcg ccggcccgac cgaggctcag gcgttcaagg
     4321 acgagctcac gcacctgctc gtgaaccaga aggcggcttt caactcaccc gtgtggttca
     4381 acgtcggctg gcgcgagcag ccgcaagtca gcgcgtgctt catcctttcg gtcgaagacg
     4441 agatgtcctc gatcctcaac tggtacaagg aagagggcct gatcttcaag cacggatccg
     4501 gtgccggcct caacctttcg aagctgcgct cgtctaaaga gcgactcaag tcgggtggga
     4561 ccgcgtccgg tcccgtctcg ttcatgcgcg gtgccgacgc gtcggcggga acgatcaagt
     4621 cgggtggggc aacccgccgg gccgcaaaga tggtcgtgct caacgtcgac caccccgacg
     4681 tcaaggactt catctggtgc aaggcactgg aagagaacaa ggcgcgcgct ctgcgtgagg
     4741 ccggtttcga catggatctc gacggcaaag actcgcattc gatccagtac cagaacgcga
     4801 acaactccgt gcgggtcacc gacgacttca tgtcggcggt catggacgac gccgagtggc
     4861 agtttcgtgc ggtcaccgat gggagcccgg tcgagaccgt aaaggccaag gagctgatgc
     4921 gagagatcgc tcaggcggcg tgggagtgcg cagaccccgg cttccagtac gacaccacca
     4981 tcaacgaatg gcacacgtgc ccgaacaccg atcggatcaa tgcttcaaat ccgtgctccg
     5041 agtacatgca cgtcgacaac tcgagctgca acctggcgtc gctgaatctg atgaagttcg
     5101 tcgactccga caacaccttc gacatcgacg gctacaagca tgcgatcgag gtcatgttcc
     5161 tggcgcagga gatctccgtc ggcttcgcct cgtacccgac cgacaagatc cgcgagaaca
     5221 cgatgaagat gcgccagctc ggccagggct acgcgaacgt cggcgcacta ctgatggctc
     5281 agggacttcc ttacgactcc gaggagggcc ggtcgtgggc ggcgtcgctc atggcgatca
     5341 tgacggggca ctgctacgcc acctcggcca agatcgccga gcgcgtgggc gtgttcgagg
     5401 agtacccgaa gaactccgac ccgatgctcg ctgtgatgcg caagcatcga gaagaggctt
     5461 acaagatcgc cgaggacaag gcaccgccgg agctcgccca ggcagcacga gccgcgtggg
     5521 acgacgccgt gcgtctcggc gaacagcacg gctaccgcaa cgcgcaggcg accgtgctcg
     5581 cccccacggg cacgatcggt cttctgatgg attgcgacac gaccgggatc gagccggacc
     5641 tcgctctgaa gaagatgaag aagctcgtcg gcggcgggac gatgtcgatc gtcaaccaga
     5701 cggttcctcg tgcgctgcgc aagctgggct acaacgagac gcaggttgcc gacatcgtga
     5761 ggttcatcga cgagaactcg cacgtcgtgg acgcgccgca cctccggaac gagcacctgc
     5821 cggtgttcga cacggcgatg ggcgagcggt cgatccacta catgggtcac atccggatga
     5881 tggctgccgt gcagccgttc atctctggcg cgatctccaa gaccgtgaat ctgcccgagg
     5941 acgtgacggt cgaggatgtt gaagaggcct acatggcggg ctggcgcatg gggctcaagg
     6001 cgatcgcgat ctaccgggat aactgcaagg tcgggcagcc gctgaacatc gccaagaagg
     6061 acgacaaaga ggcgaaggtc gaagcgcgca cgcccgagcg tcgccggctg ccgaagaagc
     6121 gcaactcgaa gacgatcaag ttccgggtcg ccgacaccga gggctacctg aacgtcggcg
     6181 agtacgacga cgggtcggtg ggcgagatct tcctcaaggt ggccaagcaa ggctcgacct
     6241 tgtcggggat catggatgcg ttcgccatct cggtctcgat ggggctccag tacggcgtgc
     6301 cgttggcggc ttacgtgaag cagttcaaag acactcgctt cgagccctcg ggcatgaccg
     6361 acgaccccga cttccggttg gccacctcga tcatcgacta cgtcttcagg gtgctggctc
     6421 tcgagtacat gtcggatgaa gagcgccacg ctctgaacat caggacggcc ggtgagcgca
     6481 aggccgagat cgaaacgaag ctcgatccca acgcaacggg ggacaccccg aacgggaatg
     6541 ggaacggcaa cgggaatggg aacggcaacg gtgcgcacga cgagaaggtg atcgtgctgg
     6601 ggccggtggc aagcgcgatc tcggactcgc ttccgttctg cggccagtgc ggggtgcaga
     6661 tgaacccggc cggctcgtgc ttcgcctgtc catcctgcgg atcgacaagt ggctgcagct
     6721 aaaaaacctc ccctgtgcag cgagtgcggg gccgtgctca ctaccggtcc cgcccgttgc
     6781 ccgctctgcg gtaccgaacc aagccagagc aagccggcgg cgaagaagag ccaggtctac
     6841 gaccgcgata agtaccaggc cgcgatccgc gacctgcgaa agaagttgaa ggagatccgc
     6901 gacgaggggg ccgaagccgt ctgaccgccg tagcatcgat gcatggctcg taagccggtc
     6961 acgatcgggc tcgtcctggc gctgctatcg atcccccttg ctacgaacgc ggccccctac
     7021 gggaagccgt acttctacac gttcagttcc cgctacaccg agggcgagac gatcaccttc
     7081 aagctccggc acgatcagca cacgaaggtg atgcggatgg gcgagacgtg ggacgtggtc
     7141 gacgcggaag gaagcgtgct ggcgcagtac tactgggaag acgaccaacg ctggctccct
     7201 ccgcatcagt accggttgtg ggagtgggac cagcatcaag cctgttcggg tgcctgccag
     7261 aacgtctggg agggcgagct ggtcccgccc gggcgctacc ggatcgtgac gaccgtcgac
     7321 gaccaggagg tggcgaagtg gttctcggtc ggcgcgttct tccacctcgg gttcgacggc
     7381 cggcccgcta ccgacttcgt ggtgttctcg aacaacgccg atgtcgtcgg gcagctgcgg
     7441 gccgagctcg accactcaca ggaggaacgc cagatcgttg cggggatcgt tcgcactcgg
     7501 aagcccggtt acaacaccga ctggtccttc gtgctcgacc cgccctcgat cttcctcggg
     7561 gaggtattcg tcgaggtgtg cgacggggct cccagataca tcgagcgaca ccgcaacgag
     7621 tggcggggcc aacagtggtg tccgtggtcg tcgtacgttg cggccgaagg tctatagatg
     7681 ctctgcccct actgcagcgc cgacgaagac aaggtcgtcg attcgcgtct ggcggaagag
     7741 ggccgcgcga tcaggagacg gcgcgagtgc atcacctgcg gtcgtcgcta cacgaccttc
     7801 gaacgagccg aagaggtccc cctgctcgtt gcgaagcgca atggcgaaga ggagccgttc
     7861 gaacgcacca agatcatcga cggcctgcgc tccgcgtgca agaaccggcc ggtcagtgac
     7921 gccgagatag aagcactcac agatgacgtc gaggaagcga tgcgcgccga cccgcgcaga
     7981 ccggttccct ccgccgagat cggtagggaa gtgctggagc gtttaaggga gctcgacgac
     8041 gtcgcctacg ttcgcttcgc ctcggtctac aaggacttcc aggagctgac cgacttcgaa
     8101 caagagctcg gcatccttca gaagaagacg gcccccaaag cgtgaagctt ctagtcacgc
     8161 gcctcgaccc cgaccttccg ctcccgcgct acgcgcacga aggtgacgcc ggtctggacc
     8221 tgttggcggc cgaagacgtg gcgttgttgc cgggggcccg ggccgccgtt cctaccggca
     8281 tcgcagtcgc catccccgac ggttacgtgg gactcgtgca cgcgcgctcc gggcgctcat
     8341 tgaaagaggg tctagcgctc gccaacgcgc ccggcgtagt ggactcgggc taccgcgggg
     8401 agataaaggt gatctgcgtg aacctcgacc ccagctcac
//
LOCUS       DATMED010000446         3562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3440209, whole genome shotgun sequence.
ACCESSION   DATMED010000446 DATMED010000000
VERSION     DATMED010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440209"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VNP73_11705"
     CDS             <1..1292
                     /locus_tag="VNP73_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL66631.1"
                     /translation="AHPEAGPLVTAASSAAGGPIAGPSPLRRPEFRRLLGISITVALG
                     FGMVIPTLPLFASSFGVGLAAIGLVQLVFGLTHFSFGIVAGLVVDRFGDRACTMAGLL
                     IVSLSSYAMGFSETFVQLVLARGFGGAGSALFIAGLMNRILRLIEPEAMGRATGAFRS
                     SFLVGIAAGPVMGGVVAKYLGLVWVFHIYASGLIVATVIAWFVMRGQRVVVIQDRKTP
                     LDALRAAKPLFGDIRYVVALLATLVGWWTLSGPTQIVGTIYAKQELGFSLEQIGLALT
                     LVAIGEVLVLFVAGRATDRFGRRAVLVPALAVSALGVAALGQIEGIPWAFYPLMLLIG
                     TGTAAGGTAAGGLLADSIPEGGSGTAVGVNQMAGDLGYLIAPTAVGFVAETQGFPLAY
                     LLAAVPAVVVFVAALKLPRGIVAATREQPPEPGIPVA"
     gene            1430..2158
                     /locus_tag="VNP73_11710"
     CDS             1430..2158
                     /locus_tag="VNP73_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66632.1"
                     /translation="MSLRALRGLPTALVGMFVAAVLFTAFTATSSNAQMPADKFAVTG
                     SDVDEVNDNTTILAEQMRVSSPADLILQVTAECSILTQLLTNNEQPASDVFGSVRLRV
                     EIDGVNVPVATNDIVGGGGADDTPNPDVGEGDDDDEVGEVTFCNRAYGREVTDGEDPA
                     DGIDSQDDYIATRAANAFNWIALNAGGDVYDADGDNILNIAVIADYDVETTGMGNVAE
                     AFVGSRTLVVEPTHLAVNETTDQE"
     gene            2240..3343
                     /gene="purM"
                     /locus_tag="VNP73_11715"
     CDS             2240..3343
                     /gene="purM"
                     /locus_tag="VNP73_11715"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWL66633.1"
                     /translation="MGTPSYEDAGVKDQGEAISAVERLLGPTFRFPRDAEVITRFGHY
                     ASVLKVAPDLAIAVSTDGAGSKTMIASALDRYDTIGFDCMAMNVNDLICVGARPIALV
                     DYVGVHTLESHRMSEILSGLAAAAEVAGVAIPGGEVAQLPDIIGSDGRSPGDERAFDL
                     VGTAVGVLHPDKLILGREITPGDAIIGIHSTGIHSNGMTLARKVLLETGGYELTEHFE
                     LLGRTLGEELLEPTAIYVRAVTDLWEKGIDTRGLVHITGDGLTNLCRLEARVGYSIEQ
                     LPEPSPIFGLIQDVGNVPEEEMFRVFNMGVGFVVVVPTEQADDALGVITAAGHEAQRL
                     GSVTEGGGEVRLESAGLVGALVEGKSTFTKVLD"
     gene            complement(3350..>3562)
                     /locus_tag="VNP73_11720"
     CDS             complement(3350..>3562)
                     /locus_tag="VNP73_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66634.1"
                     /translation="QAEKAPEAEETPEPQPSEPTPSEEPQPQETEEPPPDDDDDADAL
                     DDDDDQGEAEDDDGLPLPDDDTGGTI"
BASE COUNT          572 a         1157 c         1156 g          677 t
ORIGIN      
        1 gagctcatcc agaagccgga ccactagtga cggccgcatc ctcggccgcc ggtgggccta
       61 tcgccggacc ctctcccctt cgtcgtcccg agttccgccg actcctgggg atctcgataa
      121 ccgtcgctct gggcttcggg atggtgattc cgacccttcc cctgttcgcc agctcgttcg
      181 gtgtggggct ggccgccatc ggactcgtcc aactcgtgtt cggacttact cacttctcgt
      241 tcgggatcgt cgcgggcctc gttgtagacc ggttcgggga ccgcgcctgc accatggcgg
      301 gcctcctgat cgtgtcgctg tcctcctacg cgatgggctt ctccgagaca ttcgtgcagc
      361 tcgtgctggc gcgcggtttc gggggagccg gctcggcgct gttcatcgcc gggctgatga
      421 accggatcct ccgactgatc gagcccgaag cgatggggcg tgccaccggg gcgttccgct
      481 cgtccttcct ggtcgggatc gccgcgggtc cggtaatggg cggggtggtg gcgaagtacc
      541 tgggcctcgt gtgggtgttc cacatctacg cctcaggact catcgtcgcg acggtgatcg
      601 cctggttcgt gatgaggggg caacgggtgg tcgtgatcca ggaccggaaa acgcccctgg
      661 acgcccttcg agccgccaag cctctgttcg gagacatccg ttacgtggtg gctcttctgg
      721 ccacgctcgt cgggtggtgg acactatcgg ggcccaccca gatcgtcggc acgatctatg
      781 cgaagcagga gctgggcttc tctctcgaac agatcgggtt ggctctcacc ctcgtcgcga
      841 tcggtgaggt actcgtcctg ttcgtcgccg gacgggcgac cgaccggttc ggccggcgag
      901 ccgtcctcgt tccggcgctt gcagtgagcg cattgggtgt tgcggccctc ggtcagatcg
      961 aggggatccc gtgggccttc tatcccctca tgctgctgat cgggacggga accgcggccg
     1021 gtgggaccgc agcggggggc ctcctcgccg actccatccc cgaaggagga tcgggtaccg
     1081 ccgtcggcgt gaaccagatg gccggagatc tcggctacct gatcgcccca acggccgttg
     1141 gtttcgtggc cgagacccag ggcttccccc tcgcctatct cctcgccgcc gtgccggccg
     1201 tggtcgtttt cgtcgcggct ttgaaacttc cacgcgggat agtcgcagct acgcgcgagc
     1261 agccccccga acctgggatc ccggttgcct aatgtcccat ttgtgaccga tttgagaatt
     1321 ttttgaaaaa acttccgcta cccggaaggt gaccgagcac ctacggcgta atccttcggc
     1381 agtcgtcccg gaagcgggtc cgggacatct cctctgagga ggcgattgca tgtcgctacg
     1441 tgcacttcgc ggccttccca ccgctctggt tgggatgttc gttgccgcag tgttgttcac
     1501 ggccttcacg gccaccagtt ccaacgccca gatgcctgcg gacaagttcg cagtcacggg
     1561 ttccgacgtc gacgaggtca acgacaacac cacgatcctc gccgagcaga tgcgtgtatc
     1621 cagcccggcc gacctgatcc ttcaggtcac cgccgagtgc tcgatcctga cgcagctcct
     1681 tacgaacaac gagcagccgg cgtccgacgt cttcggctcg gttcgtctgc gcgtcgagat
     1741 cgacggcgtc aacgttcctg tcgccacaaa tgacattgtt ggtggtggcg gcgccgacga
     1801 caccccgaac cccgatgtgg gcgagggtga cgacgacgac gaagtcggag aggtcacgtt
     1861 ctgcaaccgt gcctacggcc gcgaggtcac cgacggggaa gaccccgccg acggcatcga
     1921 ctcccaggac gactacatcg ccacgcgtgc tgcgaacgcg ttcaactgga tcgcccttaa
     1981 cgctggcggc gatgtctacg acgcagacgg cgacaacatc ctcaacatcg ccgtcatcgc
     2041 ggactacgac gtcgagacca caggcatggg caacgttgcc gaggcgttcg tgggcagccg
     2101 cactctggtt gtcgagccga ctcacctggc cgtcaacgag accactgacc aggagtaaca
     2161 agaaccacag cagtttagaa acaccgaacc gggccggctt cgccggcccg gttctttttt
     2221 gtcgggtagc gtcggcgcgg tgggaactcc cagttacgaa gacgcaggtg taaaggatca
     2281 gggcgaggcc atctctgcgg tcgagcgcct cctaggcccg accttcaggt tcccccggga
     2341 cgccgaagtc atcacccgct tcggtcacta cgcgagcgtg ctgaaggtcg ctcccgatct
     2401 cgccatcgcg gtttcgaccg acggggccgg cagcaaaacc atgattgctt cggccctcga
     2461 tcgctacgac acgatcggtt tcgactgcat ggcgatgaac gtcaacgacc tcatctgcgt
     2521 gggggctcga ccgatagcgc tcgtcgatta cgtcggcgtg cacaccttgg aatcgcaccg
     2581 catgtcggag atcctcagcg gcttggccgc agcggccgag gtggccgggg tcgcgatccc
     2641 cggaggcgag gtcgcccaac tccccgacat catcggctcg gacggtcgct cccccggaga
     2701 cgagcgggcc ttcgacctgg tgggcaccgc ggtcggagtg ctgcatccgg acaagctgat
     2761 cctgggtcgg gagatcaccc ccggcgatgc catcatcggt atccactcga cggggatcca
     2821 ttcgaacggg atgacgcttg cccgcaaggt cctcctcgag accggcgggt acgagctgac
     2881 ggaacacttc gagctcctcg gccgcaccct gggggaagaa ctactcgagc cgaccgccat
     2941 ctacgtccga gcggtgaccg acctgtggga gaagggcatc gacacccggg gcctggtcca
     3001 catcacgggg gacgggctca cgaacctgtg tcgcttggag gctcgagtgg gctattcgat
     3061 agagcagctg cccgagccgt ctccgatctt cggcctgatc caggacgtcg gaaacgtgcc
     3121 ggaggaagag atgttcaggg tcttcaatat gggtgtcggc ttcgtcgtcg tcgttcctac
     3181 ggagcaagca gacgacgccc tcggcgtgat caccgcggcc ggccacgagg cacaacggct
     3241 gggaagtgtc acggagggtg ggggcgaagt cagactcgag tccgccgggt tggtcggagc
     3301 actcgtagaa ggaaagagca ccttcaccaa ggtgctcgac tagagcggct tagatcgtcc
     3361 cgccggtgtc gtcgtccggg agcggaaggc cgtcgtcgtc ctcagcttcg ccttggtcgt
     3421 cgtcgtcgtc gagggcgtcg gcatcgtcat cgtcatcggg aggaggctcc tcggtctcct
     3481 gaggctgggg ctcctcggag ggcgtcggct ccgacggctg cggctcgggc gtctcttcgg
     3541 cctcgggagc cttctcggcc tg
//
LOCUS       DATMED010000447         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1118447, whole genome shotgun sequence.
ACCESSION   DATMED010000447 DATMED010000000
VERSION     DATMED010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118447"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VNP73_11725"
     CDS             complement(<1..477)
                     /locus_tag="VNP73_11725"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWL66635.1"
                     /translation="MSPGKPFGPGERALLIDSKERRYLVILEAGKQFHSHMGTLEHDA
                     LIGELEGVRLQASRGGRLLAVRPTLSDFVLKMKRGAQVVYPKDIGLILVYADIYPGAS
                     VLEAGTGSASLTMALARAVGESGRVISYELRQDHHDQAVANVHAWFEAIGGMPPNLD"
     gene            complement(479..1480)
                     /locus_tag="VNP73_11730"
     CDS             complement(479..1480)
                     /locus_tag="VNP73_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL66636.1"
                     /translation="MKCKVCREHAVVDIRRHNAGFCRDHFLEHTRNQVARAIKEFEMF
                     RTDDRLLLAVSGGKDSLALWDILIDLGYDVTGFYLDLGIGGYSNRSKELSISFAEKRS
                     AKLIVRSIETEHGFTVPELSALTGRVPCSGCGLSKRYEFNRAALEEGFEILVTGHNLD
                     DEVATLFGNVLHWNTEMLARQMPVLNERILGEGDDAHVALVRKAKPLVKLLERETAAY
                     ALLRGIDYVIEECPMVEGNTQHQYKHAITQLEESSPGTKSQFYAGFLRKAADRFREAA
                     KDSDDIEIVACISCGAPTVLRDTHESRCSFCKTKALATKRKAGLARRRPARRGKSGA"
     gene            complement(1477..1686)
                     /locus_tag="VNP73_11735"
     CDS             complement(1477..1686)
                     /locus_tag="VNP73_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWL66637.1"
                     /translation="MRVRLRNPDRVVEVPGPKTVVAMLNELDIVPESVLVIKDKTLLT
                     RDERVDDSDEVEVRPVLSGGAGDRK"
     gene            complement(1737..>2653)
                     /locus_tag="VNP73_11740"
     CDS             complement(1737..>2653)
                     /locus_tag="VNP73_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWL66638.1"
                     /translation="VVGLGESFGGDEARAQGSGVIIDESGYIVTNNHVIEGAADVEIV
                     FTDESLDDRRVQGRVIGTDPAKDLAVILVDVDGLKSIELGSEDGLRLGDDVIALGFPL
                     GLGGATVTAGIVSAKERDISVGAGQGVDQEQLEGVLQTDAAINPGNSGGALLDSAGRL
                     VGINTAAAAASTAENVGFAISIDTALPIIKKIIENPAEKKAWLGVNIADPTDPRVEED
                     FEDVADDTKGAGLVEVFPDGPADKAGVDAGEIIVAIDAEDISSPEDLTDVLADFDPGD
                     EVTLTLAGPDGEREVTLELEERPVTLGG"
BASE COUNT          457 a          871 c          791 g          534 t
ORIGIN      
        1 gtcgaggttc ggtggcatcc caccgattgc ctcgaaccat gcatgaacgt tggcaaccgc
       61 ttgatcgtga tgatcctgtc tcagttcgta ggagatgacc cggccgctct cccccaccgc
      121 tctcgcgaga gccatggtca gcgaggccga gccggtccct gcctccagca ccgatgcgcc
      181 cgggtaaatg tccgcataga cgaggatgag cccgatgtcc ttcggataga cgacctgggc
      241 cccgcgtttc atctttagta cgaagtcgct caaggtcgga cgcactgcaa gaagacgtcc
      301 ccctctggaa gcttgcaacc gcacaccctc gagctcgccg atcagcgcgt cgtgctcgag
      361 cgttcccatg tgggaatgga attgcttgcc ggcctcgagg atcaccagat agcgccgctc
      421 cttcgagtcg atcagcaagg cgcgttctcc cggcccgaag ggtttgccgg ggctcacgtc
      481 aagccccgct cttccctcgg cgcgcggggc gtctgcgtgc cagccccgcc ttcctcttcg
      541 tagcaagggc tttcgtcttg cagaacgagc accgagactc gtgggtgtcg cgcagaacgg
      601 ttggggctcc gcacgaaatg cacgcgacga tctcgatgtc atccgaatct tttgcggcct
      661 ctcgaaagcg gtcggccgcc ttgcggagga agcccgcgta gaactggctc ttcgtcccgg
      721 gggaactctc ctcgagctgc gtgatcgcgt gcttgtactg gtgctgcgtg ttgccctcga
      781 ccatcgggca ctcctcgatc acgtagtcga tgccgcgcaa cagagcgtag gcggcggtct
      841 ctcgttccaa cagtttcacg agcggcttcg ccttgcgtac cagagcgacg tgcgcatcgt
      901 cgccctcgcc caggatccgt tcgttcagaa ctggcatctg gcgcgccagc atctctgtgt
      961 tccagtgcaa gacgtttccg aacagtgtcg caacctcgtc atcgaggttg tggccggtga
     1021 caaggatctc gaagccctcc tcgagcgcgg cgcgattgaa ctcgtagcgt ttgctcaatc
     1081 cgcagcccga gcagggaacc cgtccggtca acgcggacag ctcagggacc gtgaagccat
     1141 gctcggtctc gatggaccgg acgatgagct ttgccgagcg tttctcggcg aaggaaatcg
     1201 acaactcctt ggaccggttg gagtaaccgc cgattccgag atcgaggtag aagcccgtca
     1261 cgtcgtaccc gagatcgatg aggatgtccc agagggcgag agagtccttg ccccccgata
     1321 ccgccaggag caaccggtcg tcggtccgga acatctcgaa ctccttgatg gcccgggcga
     1381 cctgattgcg tgtgtgctcg agaaagtgat cccggcagaa gcccgcgttg tgccgacgga
     1441 tgtccacgac ggcgtgctca cggcagacct tgcatttcat ttgcgatctc ctgctccacc
     1501 tgaaagcacg ggtctgacct cgacctcgtc ggagtcgtcc acgcgttcat cgcgcgtcag
     1561 aagagtcttg tccttgatga cgagaaccga ctctggcacg atatccagct cattgagcat
     1621 ggccaccacg gtcttcggac ctggcacctc caccactcgg tcggggttgc gaagcctcac
     1681 acgcatgccc tgagagtagc tgtggcgcct ctgaagtgga aatccctgcc taacccctag
     1741 ccgcctagag tcaccggccg ctcttcgagc tcgagcgtga cctctctctc gccgtcgggc
     1801 cccgcgagag tcaatgtcac ttcatcgccc gggtcgaagt cggccagcac atccgtaaga
     1861 tcctcgggtg aggagatgtc ctcggcgtct atcgccacga tgatctctcc tgcatcgaca
     1921 cccgccttgt cggcaggccc atctggaaag acctcgacga ggccggcgcc cttggtgtcg
     1981 tcggcgacgt cctcgaagtc ttcctcgacg cgggggtcgg tcggatccgc aatgttcacg
     2041 ccaagccatg ccttcttctc ggctgggttc tcgatgatct ttttgatgat cgggagcgcg
     2101 gtgtcgatgg atatcgcgaa tccgacgttc tcggcggtgc ttgcagccgc ggcagccgtg
     2161 ttgatgccga cgagccgtcc ggccgaatcg agcagcgcgc ctcccgagtt tccgggattg
     2221 atcgccgcgt ccgtttgcag gactccctcg agctgctctt ggtccacgcc ttggccggcg
     2281 ccgacggaga tgtcccgctc cttggcggac acgatcccgg cggtgaccgt cgccccgccc
     2341 agccccagag ggaagccgag agcaatgacg tcgtctccca gtcgcaagcc gtcttcgctt
     2401 ccaagctcga tggacttgag accgtcgacg tcgacgagga tgacggcgag gtctttggcg
     2461 ggatcggttc caatgacccg cccttgaacg cgccggtcgt ccaggctctc gtcggtgaaa
     2521 acgatctcga catcggccgc accctcgatg acatggttgt tggtcacgat gtagcccgac
     2581 tcatcgatga tcacgccgga cccttgcgcc cgcgcttcgt ctccgccgaa ggattcaccg
     2641 agaccgacga ctt
//
LOCUS       DATMED010000448         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5738786, whole genome shotgun sequence.
ACCESSION   DATMED010000448 DATMED010000000
VERSION     DATMED010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5738786"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VNP73_11745"
     CDS             <1..183
                     /locus_tag="VNP73_11745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWL66639.1"
                     /translation="GGGVATLYAHLSAYDVRNGESVSQGDVVGRVGCTGSCTGDHLHF
                     EVRVNGSPVDPLDFLP"
     gene            complement(203..1132)
                     /locus_tag="VNP73_11750"
     CDS             complement(203..1132)
                     /locus_tag="VNP73_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL66640.1"
                     /translation="MKKLPPGYTWRRATREDVDRIAELTAAVDVAQGADPSYRADEIL
                     IDWNRPGFEFDQDSWMFEYEGKLAAYGHLSPRGDSDLVLMAWTHPNHDQWALSFSLMS
                     AMEPRAREIAGSSPYKRGVAIASEHEEALMAAIEAEGFKPDALFVEMVMSLDGFQLGD
                     VRLPAGVEIRQLDPAETREFYDVLVAAFAEHWGAGFAPFDEWLRDVKGLEGYDPALSL
                     VAVDAGRIVGALFSHVTEELGEIHDIGTLPDHRGRGLGEALMRYGFARLQEKGATSAR
                     LWVDKGNVTNAIRLYEKVGMSVSRKLHFYAKDL"
     gene            complement(1231..>2650)
                     /gene="groL"
                     /locus_tag="VNP73_11755"
     CDS             complement(1231..>2650)
                     /gene="groL"
                     /locus_tag="VNP73_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804390.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWL66641.1"
                     /translation="NDVAGDGTTTATVLAQAMVRAGIQNVAAGANPMSLKRGIEKAVA
                     AAVDSIKTQSREVEGREQIAHVAAISANNDPEIGEMVAEAMDKVGKDGVITVEESNTF
                     GLELELVEGMQFDKGYISPYFVTDQDRMEAIFEEPYILFAQQKISSVRDLLPVLEKVI
                     QAGKPLVIISEDVEGEALATLVVNKIRGTFTAAAVKAPGFGDRRKAMLQDMAILTGGQ
                     VISEEIGLKLESVGLDMLGQARKVVITKDNTTIVEGTGDAGEIKGRIEQIKAEIERTD
                     SDWDREKLQERLAKLAGGVAVLKVGAATEVELKEKKHRIEDSVSATRAAVEEGVVPGG
                     GATLVRAQEAVEALELSDDEAAGARIVLKALEAPLWWIASNAGLEGGVVVDKVRNEKP
                     GHGLNAATGEYVDLVATGIIDPARVTRSALENAASIAALLLTTEATVADKPEDEDDAA
                     GHAGHGHGMGGMGGMGGMGGMM"
BASE COUNT          447 a          903 c          781 g          519 t
ORIGIN      
        1 ggcggtggcg tggcgacgct ctacgcccac ttgtcggcgt acgacgttcg caacggggaa
       61 tccgtgtcac aaggggatgt cgtgggccgg gtcggctgca ctggatcgtg caccggtgac
      121 cacctccact tcgaggtgcg ggtcaacgga agcccggtag acccgctcga cttcctcccc
      181 tagacgagcg aacgcagcac ccttagagat ccttcgcgta gaagtggagc ttccggctga
      241 cgctcatgcc gaccttctcg tagagacgga tggcgttcgt cacgttcccc ttatccaccc
      301 acagccgggc tgaggtcgct cccttctcct gcaatcgggc gaacccgtag cgcatcaggg
      361 cttcgcccag tcccctaccg cgatgatccg gcaacgtccc gatgtcgtgg atctccccta
      421 gctcctcggt gacgtgtgag aagagcgctc cgacgatgcg gccagcgtcc actgcgacga
      481 gggagagggc cgggtcgtat ccctcgaggc ccttgacgtc ccgcagccat tcatcgaagg
      541 gcgcgaatcc ggccccccaa tgctccgcga atgctgccac gagcacgtcg tagaactcgc
      601 gtgtctcagc cggatcgagc tgccttatct cgacccctgc tgggagccga acgtccccaa
      661 gttggaaccc atcgagggac atgaccatct cgacgaacaa cgcgtctggc ttgaatcctt
      721 cggcttcgat cgccgccatc agcgcttctt cgtgctcgga ggcgatcgcg acaccacgct
      781 tgtatggaga cgacccggcg atctcacggg cgcgcggctc catcgccgac atcaatgaaa
      841 aggacaaggc ccactggtcg tgattgggat gggtccaagc catgagtacg agatcgctgt
      901 cgccgcgcgg agacaggtga ccgtacgcgg caagcttgcc ttcgtactcg aacatccacg
      961 agtcctggtc gaactcgaac ccgggccggt tccagtcgat gaggatctcg tccgcccggt
     1021 aggagggatc ggctccctga gcgacatcga cggctgccgt caattccgcg atgcgatcga
     1081 cgtcctcacg agtggcccgg cgccacgtgt agccgggagg caacttcttc atcgccgaaa
     1141 agataagggg ccgggtgaga tacccggccc ctaacttcct tttggtgctg tccggaagcc
     1201 ctataagggc caccagttag aactcaacgg ctacatcatg ccgcccatgc cgcccatgcc
     1261 acccatgcca cccatcccgt ggccgtggcc tgcatgtcct gcagcgtcat cttcgtcctc
     1321 gggcttgtcg gcaaccgtcg cttcggtcgt cagcaagagc gctgcgatcg atgcggcgtt
     1381 ctcgagcgcc gacctcgtca cgcgagccgg atcgatgatg cctgtcgcca ccaggtcgac
     1441 gtactcgccg gtcgccgcgt tcagcccgtg accgggcttc tcgttgcgga ctttgtcgac
     1501 gacgacgccg ccctcgaggc cggcgttgct ggcgatccac cacaggggag cctccagggc
     1561 cttgaggacg atgcgagcgc ctgcggcctc gtcatcggac agctcgagag cctcgacggc
     1621 ttcttgcgcc cgtaccaagg tcgctccacc accgggaacg acaccctcct cgaccgctgc
     1681 tcgagttgca gagacggagt cttcgatgcg gtgcttcttc tctttcagct cgacctccgt
     1741 ggcggcgccc accttcagaa cggcgacccc accggccagc ttcgcgagac gctcctgcaa
     1801 cttctcgcga tcccaatcgg aatcggtgcg ctcgatctcg gccttgatct gctcgatgcg
     1861 gcctttgatc tcgcctgcgt ctccggttcc ttcgacgatc gtcgtgttgt ctttggtgat
     1921 gacgaccttg cgcgcctggc ccagcatgtc gagaccgacg ctctccagct tcaggccgat
     1981 ctcttccgag atcacctggc ccccggtgag gatcgccatg tcctgcagca tcgccttgcg
     2041 gcggtcaccg aagcccggcg ccttcaccgc cgcggcagtg aacgtgccgc ggatcttgtt
     2101 gacgacgagt gtggcgagag cctcgccttc gacgtcctcg gagatgatca cgagcggctt
     2161 tccggcctgg atcaccttct cgagaaccgg aagcagatcc cgcaccgacg agatcttctg
     2221 ctgcgcgaac aggatgtacg gctcttcgaa aatggcctcc atccggtcct gatcggtcac
     2281 gaagtaaggc gatatgtagc ctttgtcgaa ctgcataccc tcgacgagct cgagctcgag
     2341 accgaaggtg ttcgactctt caacggtgat gacaccgtcc ttaccgacct tgtccatcgc
     2401 ttcggcaacc atctcgccga tctccgggtc gttgttcgcg ctgatcgcgg caacgtgcgc
     2461 gatctgctcg cggccctcga cctcacgcga ctgggtcttg atcgaatcga ccgcagcggc
     2521 gacggctttc tcgatgcctc gcttgaggga catcgggttc gcaccggcgg ctacgttctg
     2581 gatccccgca cggaccatcg cctgagcgag aaccgttgcg gtcgtggttc cgtcaccggc
     2641 aacgtcgttc
//
LOCUS       DATMED010000449         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4204313, whole genome shotgun sequence.
ACCESSION   DATMED010000449 DATMED010000000
VERSION     DATMED010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204313"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..266)
                     /locus_tag="VNP73_11760"
     CDS             complement(57..266)
                     /locus_tag="VNP73_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWL66642.1"
                     /translation="MAESVYKIVELVGTSTESWEKAATAAVEKASKSIRNLRIAEVSE
                     LDLHIEDGKVSSFRAKVKLSFKYED"
     gene            370..>2171
                     /locus_tag="VNP73_11765"
     CDS             370..>2171
                     /locus_tag="VNP73_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66643.1"
                     /translation="MRKAPLLCLSVVMLAFGLPFVAPAAAQSPADPAGRETEPVILTG
                     TNFPEWATVGDVTLEQPHTEGAQCLAAQNGSPDEITPEETCSHNTFEDPDFSSGDYRT
                     DTGVAIDKLLGYQWNGESFDQIPFQVDEMFSRYMSNNNSGFAFYSETDQHTTYAFDYD
                     RYTWTASDPDNPCLSHPASKDDPLTPDAERHAAKDPIEGLDTDDEVVFMADDAAAQAP
                     ADAELPPGITDSYEVALTDPSAQGATRYAYVMLAGGEKPATAAFTADNGYVRYERDAD
                     ADVFLFSESSYDNYGAAPEGPWFDPATGTCHTAENEWRQRRPVDRAWIYTPRYKFRYS
                     GRWVMNELHVSDRDKDGETQTFSDLGDLPDDWYGPDLIDQWKARAFQQRPSGQTPCCG
                     YEEEVNNWGGSSMLMGERVGPVRAIRESWGADSSTNLARREIFYRDEIRFSSFLRVHV
                     IPPLDGIYAQWDYNLGTVEKYYNPIVSEGVDIDGQNDEAFGNSRLHIGRDGVSYDGDD
                     TASDQIDEATGSDTQSVGNPNEPDCSYPGDPAEAEWDDFRRQIPDPIGGELPGDVVDL
                     CIYNDIDSPDPAFSGVNAGLNWEEIAGENGTLVLR"
BASE COUNT          415 a          739 c          645 g          372 t
ORIGIN      
        1 cgagcgctac gacgccgcgg agacgatcac ctctataagg atcggtcgct aggcccctag
       61 tcctcgtatt tgaaggacag cttcaccttg gctcggaagc tggagacctt tccgtcttcg
      121 atgtgcaagt cgagctcgga aacctcggcg atccgcaggt tgcggatgct cttactcgcc
      181 ttctcgacgg ctgccgtcgc cgccttctcc cacgattcgg tgctcgtccc taccaactcg
      241 acgatcttgt agacgctctc ggccacaact ttctccttcc agggtcgact gggcccgagg
      301 gtaccgtccg gctcgtccgg atggcccgtt gtgcaggact tgtcccccga agtggcgaac
      361 cctcggagca tgcgcaaagc ccccttgctc tgcttgagcg tcgtcatgct cgccttcggc
      421 ctcccgttcg tggccccggc ggcagcgcag agcccggccg atccggccgg ccgcgagacc
      481 gagccggtca tcctgacggg aacgaacttc cccgagtggg caacggtggg cgacgtgacc
      541 ctagagcagc ctcataccga gggagcccag tgcctcgccg cccagaacgg ctcgccggac
      601 gagatcacac ctgaagagac ctgcagccac aacaccttcg aggaccccga cttctcgagc
      661 ggggactacc gcaccgacac tggcgtggcg atcgacaagc tcctcggcta ccagtggaac
      721 ggcgagtctt tcgatcagat ccccttccag gtcgacgaga tgttctctcg ttacatgtcg
      781 aacaacaatt ccggcttcgc cttctactcg gagacggatc agcacacgac ctacgcgttc
      841 gactacgacc gctacacgtg gacggcatcg gatcccgaca acccctgcct gtcgcacccg
      901 gcctcaaaag acgatccgct gactccagac gcagagcgac acgcggcgaa ggatcccatc
      961 gagggcctgg acaccgacga cgaggtcgtg ttcatggccg atgacgccgc agctcaggcc
     1021 cccgcggacg cggagcttcc tcccggcatc accgattctt acgaggtagc gctgaccgat
     1081 ccgtcggccc aaggtgcgac tcgctacgcc tacgtgatgc tcgccggcgg agagaagccg
     1141 gcgaccgcag cgttcacggc cgacaacggc tacgtgcgtt acgagcgcga cgccgatgcc
     1201 gacgtgttcc tgttctccga aagctcatac gacaactacg gtgcggcccc cgagggtccg
     1261 tggttcgatc ccgccaccgg cacctgccac actgccgaga acgaatggcg gcagaggcga
     1321 ccggtcgatc gagcctggat ctacacgcct cgctacaagt tccgctacag cgggcgctgg
     1381 gtgatgaacg agctccatgt ctcggacaga gacaaggatg gcgagacgca gacgttctca
     1441 gacctcgggg acctccccga cgattggtat ggccccgatc tcatcgacca gtggaaggcg
     1501 cgtgcgttcc aacagcgtcc ctccggccag accccgtgct gcggctacga ggaagaagtc
     1561 aacaactggg gtggctcctc gatgcttatg ggtgaacgcg ttggaccggt tcgcgccatc
     1621 cgcgaatcct ggggagccga ttcgtcgacg aacctcgccc gccgcgagat cttctatcgc
     1681 gacgagatcc ggttctcttc cttcttgcgg gttcacgtga tcccgccttt ggacgggatc
     1741 tacgcgcagt gggactacaa cctgggaacc gtagagaagt actacaaccc gatcgtgtcc
     1801 gaaggcgtcg acatcgacgg ccagaacgac gaggcgttcg gaaactctcg tcttcacatc
     1861 ggccgcgacg gcgtttccta cgacggggac gacacggctt ccgaccagat cgacgaggcc
     1921 accgggagcg acacgcaatc cgttggcaac ccgaacgagc ccgactgcag ctaccccggt
     1981 gacccggccg aagccgaatg ggacgacttc aggcggcaga tccccgaccc gatcggcggc
     2041 gagttgcccg gcgatgtcgt cgacctgtgc atctacaacg acatcgacag tcccgacccg
     2101 gctttctccg gtgtaaacgc agggctcaac tgggaggaga tcgcgggcga gaacggcacg
     2161 ctcgttctcc g
//
LOCUS       DATMED010000450         3845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3050672, whole genome shotgun sequence.
ACCESSION   DATMED010000450 DATMED010000000
VERSION     DATMED010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050672"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VNP73_11770"
     CDS             <1..945
                     /locus_tag="VNP73_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HWL66644.1"
                     /translation="LRERPGPRDLYQRWEKQNWSAYEIEFSKDKEDWANFPKDLKFQL
                     MLGFRGFFLGEAAVTDTLSPLVHAAPTDDDRHFLTTQLVDEARHTIFFDRFFSEALGI
                     DGLDEVRRQTEEIGYTADRDRGYSRLFYKDLVEVTDAVRLDPSSYEKWVSGIVLYHML
                     VEGMLALAGQRRILHVARMFDVLPGFRAGFTMVTRDESRHVNYGVYAILNAVQAGLTD
                     HIIATAERHLIPTCDVLTAPFRKIPPIPPEFMKQFPIRQGWEFGIHQLRKRLRAGGVD
                     EVRLDPLEEQWWARIEANLDEYEERFDEVHPARQPIPA"
     gene            complement(991..1353)
                     /locus_tag="VNP73_11775"
     CDS             complement(991..1353)
                     /locus_tag="VNP73_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL66645.1"
                     /translation="MAITGTHVLLYTSEPEALRNVFRDVFGFDHVDGGEGWLIFALPP
                     AELGIHPAEGPTFDSGVRHQLTLMCDDIAKTVEDLKDKGIDVRGEPEDEGWGITTTLA
                     LPGGVDVMLYQPRHPTAI"
     gene            1411..3078
                     /locus_tag="VNP73_11780"
     CDS             1411..3078
                     /locus_tag="VNP73_11780"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL66646.1"
                     /translation="MDRVRDSKIRSLLFLSLMFSSLLLLAPAASDAAAACRAPGEALK
                     FDKQKYIDMTRAGGEPTVEQHPDGTLMYGSHAGTTHFYSPAAADPTTAAFVENYTGQT
                     YYYFSKDHGKTWTFQERTLPPNNSPMSGFSDPEYAIDETGQVYVSEINLLNVAVSKAP
                     GSDEPFVLQNFFGQTISDRQWMEADKKDVLYLVGNELGGGTFPNDPVGNAGHYLHRST
                     DGGQTFTPGVEDGAGLGDLQIDRRDGTLYEAYYDDGVLSMTAYREARSGNLDKRETNE
                     VAKGVSMLSHWPSFDIDSAGNQYMTWDESGQGDRPAGVWFSYSTDGGRTWAKPTRVDK
                     NTNTDIWPWLAVGDKGKVAVAWLGADTKLPGHDAETQGDQKWRIYVAQTLNGLGCKGS
                     SAPGFRVTTATSRPVHTSTICQGGTACQAAAIDRRLGDFFTIEIDNSGHVWGGYSDTA
                     EGGATALPGFVHQSGGIPFIKQRAGAATNGSGAGASDAGGSTQNSGGDSSAGGAQQAD
                     ADSEVAGSAQEPSSLAATGAAILGFVALGLALVVGGWYIKRHSATRV"
     gene            3084..3563
                     /locus_tag="VNP73_11785"
     CDS             3084..3563
                     /locus_tag="VNP73_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66647.1"
                     /translation="MRRVSGASIVVALLVASLGACTGDTNDIRPAAACRLPKTLDSPP
                     EFPADLPWPGDVRLTQAKVNKQFVSVEGYSKRSVEHLFTSVRPHLTRNGFDIINTDYE
                     GFEAELFIAKGDGLAGIVSLREALCDGYVKVNVLYDPLETSAGREAVRKTRKLSGEG"
     gene            complement(3567..>3845)
                     /locus_tag="VNP73_11790"
     CDS             complement(3567..>3845)
                     /locus_tag="VNP73_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HWL66648.1"
                     /translation="LSIPVFAVGGIRGLDECLGILENDEADMVGIGRPFYAEPDLPAR
                     LLAGDREKAFCESSNRCVPAQQLGMKARCYNSNVARRLAERREKTAAR"
BASE COUNT          730 a         1211 c         1187 g          717 t
ORIGIN      
        1 ttgcgcgaac gaccgggacc gcgcgacctc taccagcggt gggagaagca gaactggtcg
       61 gcatacgaga tcgagttctc caaggacaaa gaggactggg cgaacttccc gaaggatctg
      121 aagttccagc tgatgctcgg cttccgcggt ttcttccttg gcgaagctgc agtgacggac
      181 acactgtcgc cactcgtgca tgcagctccc acagacgacg atcgccattt cctaaccact
      241 caactcgtgg atgaagctcg gcacacgatc ttcttcgacc gcttcttcag cgaagctctt
      301 ggtatcgacg gactcgatga ggttcgtcgt cagacggagg agatcggcta caccgccgac
      361 cgcgatcgtg gctacagccg gctgttctac aaggacctcg tcgaggtgac cgacgcggtg
      421 cggctcgacc cgagcagtta cgagaagtgg gtctccggca tcgtgctgta tcacatgctc
      481 gtcgaaggca tgcttgccct cgccggacaa cgtcgcatcc tccacgtcgc gcgcatgttc
      541 gacgttctgc ccggattccg cgcgggtttc acgatggtga cccgcgacga gtcacgtcac
      601 gtgaactacg gtgtctacgc gatcctgaac gcggtgcaag caggtctgac ggatcacatc
      661 atcgccactg cggagcgtca tctcatccca acctgcgacg tgctcacggc gccattccgc
      721 aagatcccgc cgatcccacc cgagttcatg aagcagttcc cgatccggca gggctgggag
      781 ttcggcatcc accagctgcg caagcgactt cgtgccggcg gggtcgacga ggtgcgcctc
      841 gacccactcg aagagcagtg gtgggcgcgg atcgaggcca acctggacga gtacgaggag
      901 cgcttcgacg aagtccaccc cgcccgccag ccgatcccgg cctaactcct ttgttctaac
      961 tggtggcccc tatagggccc tgaaccgata tcagatcgcg gtgggatggc gcggttggta
     1021 gagcatcacg tcgactccac ccggtagcgc gagtgtcgtg gtgatgcccc aaccctcgtc
     1081 ctccggttcg ccgcgtacgt cgatcccctt gtccttgagg tcctcgaccg tcttggcgat
     1141 gtcgtcacac atcagcgtca actggtgacg aactccactg tcgaacgtag gaccttcggc
     1201 cggatggatg ccgagttcgg ccgggggcag ggcgaagatc agccatccct cgccaccatc
     1261 cacgtgatcg aacccgaaga catcgcgaaa cacgttgcgc agcgcctcag gctcggaggt
     1321 gtagagcaga acgtgcgtcc cggtgatggc catctttgga ttctaagagg cgtactctgg
     1381 cccttcggct aggcacccgg gggtggcctt atggatcgtg ttcgcgactc gaagattcgg
     1441 tctctgctgt tcctttcgct catgttctcg agccttctcc tgttggctcc ggcggcatcg
     1501 gacgctgctg ccgcttgtcg ggcccccggc gaggccctca agttcgataa gcagaagtac
     1561 atcgatatga ctcgagcggg cggcgagccg acggtcgagc agcaccccga cggaaccttg
     1621 atgtacggat ctcacgccgg gacgacccac ttctactctc cggccgcagc ggaccccacg
     1681 accgcagctt tcgtcgagaa ctacaccggc cagacctact actacttcag caaggatcac
     1741 gggaagacgt ggaccttcca ggaacggact ctccctccga acaactcacc tatgtcgggc
     1801 ttctccgatc ccgaatacgc gatcgacgaa accgggcagg tttacgtctc ggagatcaat
     1861 cttctgaacg ttgctgtctc aaaggccccg gggtcggacg aacctttcgt gctgcagaac
     1921 ttcttcggcc agaccatcag cgatcgccag tggatggagg ccgataagaa ggacgttctc
     1981 tacctggttg gcaatgagtt gggcggtggc accttcccca atgatcccgt tgggaacgcg
     2041 ggccattact tgcaccgcag taccgacggt ggtcagacgt tcactcccgg tgtcgaggat
     2101 ggcgcgggac tcggcgatct ccagatcgat cgccgtgacg gcaccctcta cgaggcgtac
     2161 tacgacgacg gcgtgctgtc gatgaccgcc tatcgcgagg cgcgctcggg caatctcgac
     2221 aagagagaga cgaacgaagt agccaaaggc gtcagcatgt tgtctcactg gccctcgttc
     2281 gacatcgact ccgccggcaa ccagtacatg acttgggacg agagcgggca aggcgatcgt
     2341 ccggccgggg tgtggttctc gtattcgacc gacggcggca ggacctgggc caagccgacc
     2401 cgggtcgaca agaacaccaa taccgacatc tggccgtggc tcgccgttgg tgacaagggc
     2461 aaggttgcgg tggcgtggct gggtgccgac acgaagcttc cgggtcatga cgcggagaca
     2521 caaggcgatc agaagtggcg catctatgta gcccagaccc tcaacggttt gggatgcaag
     2581 ggttcgtcgg ctcccgggtt cagggtcaca accgcgacct cccggcccgt tcacacgagc
     2641 acgatctgcc agggcggaac cgcgtgtcag gcggccgcga tcgaccgccg gctcggcgac
     2701 ttcttcacga tcgagatcga caactccgga cacgtgtggg gcggttactc cgacaccgcg
     2761 gagggtggcg cgaccgcgct tcccggcttc gtccatcagt cgggtgggat tccctttatc
     2821 aagcaacgag ctggggctgc cacgaacgga tctggcgctg gagcgagcga cgctggagga
     2881 tcaactcaga actccggtgg cgactcatcc gctggtggtg cgcaacaggc tgacgccgat
     2941 agcgaagtgg ctggatccgc gcaggaaccg tcgagtcttg cggcgacggg ggcagcgatc
     3001 ctcgggttcg tggccctcgg acttgcgctc gtggttggcg gttggtacat caagcgccac
     3061 tccgcgacca gggtttgatc gcgttgcgcc gagttagcgg cgcatcgatc gtagtcgcgc
     3121 tgctcgttgc cagcttgggc gcatgcaccg gcgatacgaa cgacatacga ccggctgcgg
     3181 cgtgcaggct gcctaagacg ctcgattcac ctccggagtt tcccgccgac ctcccgtggc
     3241 ccggcgacgt ccgcctgacc caggcgaagg tcaacaagca gttcgtctcg gttgagggct
     3301 acagcaagcg atcggtcgag catctcttca cgagcgttcg gccccatctc acccgcaacg
     3361 gcttcgacat catcaatacc gactacgagg gtttcgaggc cgagctcttc atcgctaaag
     3421 gtgacgggct tgcggggatc gtttctctga gagaagcgct ctgcgacggc tacgtcaagg
     3481 tgaacgttct ctacgacccg ctcgaaactt ccgcaggccg ggaagcagtc agaaagacga
     3541 ggaagctctc cggcgagggc tagggactag cgggcggcgg ttttctcccg gcgttcggcc
     3601 aggcggcgag cgacgttcga gttgtagcag cgtgctttca tccctaactg ctgggccggg
     3661 acgcatcgat tcgacgactc gcagaatgcc ttctcccggt ctcccgcaag caatctcgcc
     3721 ggcagatcag gctcggcata gaacggacgg ccgatcccaa ccatgtcggc ctcgtcgttc
     3781 tcgaggatgc cgaggcattc gtcgagaccc cggatcccac cgacggcgaa caccgggatc
     3841 gataa
//
LOCUS       DATMED010000451         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5744114, whole genome shotgun sequence.
ACCESSION   DATMED010000451 DATMED010000000
VERSION     DATMED010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5744114"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1436)
                     /locus_tag="VNP73_11795"
     CDS             complement(141..1436)
                     /locus_tag="VNP73_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL66649.1"
                     /translation="MSPKSIFYVILDGLGDDPLPELGSKTPLEAARTPLLDRLAAQGR
                     NGYVTTVGEGIAPESDIAVFSILGYDPHEHHTGRGPLEAVGAGLKVETGDLAYRVNFA
                     TVEADGDGWTIVDRRVGRDLSSEEAHALAEEVQTTVRLDKASFDFRATIGHRGVLVLR
                     SEEGPLSAEVENSDPAYGRQGALGVAKETFDNKVVHVTPIEGHGSDAAAVRGAELTNS
                     WLRSAYEVLNGSEVNRARVAAGKMPGNFILTRDGGDHIPDLVPFKEKFGLSMGCFVEM
                     PVELGIARLMDMGVVEAPTDLPPIEQYEAWAELALEAIEGYDGLYIHIKGPDVPAHDG
                     DYEAKIASIEEIDSAFFGPLLDGLDRARSIVAVTADHSTSCARKAHTDGPVPLLISGG
                     GVSSDGVATYGETASRGGSLGHLKGVEIMPNLVAVARYA"
     gene            complement(1433..2230)
                     /gene="tpiA"
                     /locus_tag="VNP73_11800"
     CDS             complement(1433..2230)
                     /gene="tpiA"
                     /locus_tag="VNP73_11800"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWL66650.1"
                     /translation="MAEGRKPLIAGNWKMNKTHLEAMHLVQQLGHELATHDFDQVEVC
                     VCPPFTSLRTIQTLIDADRYRFGLGAQNMHWEKDGAFTGEASATMLKSLDVSYVILGH
                     SERRELFGETDDNVNKKVKVAFEAGLIPIMCCGETDGEREAGETEAKVERQVRAGLAG
                     IKPEQAGRAVIAYEPIWAIGTGKTATPEDAQSTIAHIRKVVSAAIDENTSSHVRILYG
                     GSVKAGNAASLMGQADIDGALVGGASLEAAEFSAIVKATPGGGSARD"
     gene            complement(2223..>2637)
                     /gene="pgk"
                     /locus_tag="VNP73_11805"
     CDS             complement(2223..>2637)
                     /gene="pgk"
                     /locus_tag="VNP73_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWL66651.1"
                     /translation="VDVVAATAPEEGAEHAEVALAEIGDRLGVDIGPQTRTAFSEVIS
                     SARTVLWNGPMGIFEIDDFARGTEAVAEAVTTATKNGAYTVAGGGDSAAALKKLDMTD
                     AVSHLSTGGGASLEFLEGKELPGIAVLRKKGGRGG"
BASE COUNT          446 a          917 c          762 g          512 t
ORIGIN      
        1 gacttccatg atctcggtct cgagggggcc gagtacgtcg gctagggaat gcttacggcg
       61 agacggcacg gagccaatct tagtcccgcc cggctaaaga gcgccccctc ggaggcctaa
      121 agttccgcgg aagtagggac ctaggcgtat cgcgccacgg caacgaggtt cggcatgatc
      181 tccacaccct tgaggtgacc gaggctcccc ccacgcgacg cggtctcgcc gtaggtagcc
      241 acgccgtccg acgacacccc acccccggag atcaacaacg ggaccggccc gtcggtgtga
      301 gccttgcgag cgcaagacgt cgagtggtct gcagtcacgg cgacgatgct gcgcgcgcga
      361 tcgagcccgt ccaacagcgg cccgaagaac gccgaatcga tctcctcgat gctcgcgatc
      421 ttcgcttcgt agtcgccgtc gtgagccggt acgtcgggtc ccttgatatg gatgtagagg
      481 ccgtcgtacc cttcgatcgc ctccagcgcg agttccgccc acgcttcgta ctgctcgatc
      541 ggcgggaggt cggtaggcgc ctcgaccacg cccatgtcca tcagcctggc gatacccagc
      601 tcgacgggca tctccacgaa acaccccatc gagagtccga acttctcctt gaagggaacg
      661 agatcgggaa tgtgatctcc cccatcgcgc gtcaggatga agttccccgg cattttgcct
      721 gccgcaacac gagcacggtt gacctcggat ccgttgagca cctcgtacgc ggacctgagc
      781 cacgaattcg tcagctctgc gccgcgcacg gccgcggcat cggatccatg tccttctatc
      841 ggggttacat ggacgacttt gttgtcgaac gtttccttcg caaccccaag ggcgccctgg
      901 cgcccgtagg ccggatccga gttctcgacc tcggccgaca gcgggccttc ttccgagcgc
      961 agcaccagca caccccggtg gccgatcgtg gcacggaaat cgaacgacgc tttgtcgagc
     1021 cgcaccgtgg tctgaacctc ttcggcgaga gcatgtgctt cctcggatga aagatccctg
     1081 cccaccctgc ggtccacgat cgtccagccg tcgccgtcgg cctcgacggt tgcgaagttg
     1141 accctgtacg cgagatcacc cgtctcgacc ttcaatcccg cgccgacggc ttcaaggggg
     1201 ccgcgtccgg tgtgatgctc gtgtgggtcg tagccgagga tcgagaacac ggcgatgtcc
     1261 gactccggcg cgatcccctc gcccaccgtg gtgacgtaac cgttccttcc ctgggccgcg
     1321 agtcgatcga gcaaaggggt gcgcgccgct tcgagcgggg tcttgctacc gagctccggc
     1381 agcgggtcgt cgccgagtcc gtccaggatg acgtagaaga tgctcttagg actcaatctc
     1441 tcgccgaccc cccaccgggc gtcgccttga cgatcgccga gaattcggcc gcctcgagtg
     1501 aggctccccc gacgagggcg ccgtcgatgt cggcttgccc catgagactc gcagcgttac
     1561 cggccttgac actgcccccg tacaggatgc gaacgtggct cgacgtgttc tcgtcgatcg
     1621 cggcggatac gaccttgcgg atatgggcga tcgtggattg cgcgtcttcg ggggttgcgg
     1681 ttttgcccgt cccgatggcc cagatcggct cgtacgcgat cacggcccgt ccggcctgct
     1741 ccggcttgat accggcgagc cccgcgcgca cctggcgctc gaccttggct tccgtctcgc
     1801 cggcctctcg ttccccgtcc gtctctccac aacacatgat cgggatcaat cccgcctcga
     1861 aggcaacctt gaccttcttg ttcacgttgt cgtccgtctc gccgaacaac tccctgcgct
     1921 cgctgtgacc gaggatcacg taggaaacgt ccagggactt cagcatcgtc gcggacgctt
     1981 caccggtgaa tgcgccgtcc ttctcccagt gcatgttctg cgcgcccaga ccaaagcgat
     2041 accggtcggc atctatcaga gtttggatcg ttcgcaacga cgtgaacggg gggcacacgc
     2101 agacctcgac ctgatcgaag tcgtgggttg cgagctcgtg ccccagctgc tgaaccagat
     2161 gcatcgcttc cagatgcgtc ttattcatct tccaattgcc cgcgatcagg ggcttccgtc
     2221 cttcagccac ctcgtccgcc tttcttcctc aggaccgcga tgcccgggag ttccttgcct
     2281 tcgagaaact ccagagatgc gcctccgccc gtcgacagat gcgataccgc atccgtcatg
     2341 tccaacttct tcagggccgc cgcggagtcg cctccgccgg ccactgtgta cgcgccgttc
     2401 ttcgtcgcgg tagtgacagc ttccgccacc gcctccgtcc ccctagcgaa atcatcgatc
     2461 tcgaatatcc ccatcggacc gttccacaac acggttcgtg ccgaagagat cacttccgag
     2521 aaagccgtcc tcgtctgcgg gcctatgtcg acaccgagac gatcacctat ctcggcgagg
     2581 gcaacttctg catgttccgc gccttcttcc ggagcagtcg cagcgacgac atcgacc
//
LOCUS       DATMED010000452         8853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3448164, whole genome shotgun sequence.
ACCESSION   DATMED010000452 DATMED010000000
VERSION     DATMED010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3448164"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1534)
                     /locus_tag="VNP73_11810"
     CDS             complement(161..1534)
                     /locus_tag="VNP73_11810"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWL66652.1"
                     /translation="MIGLKLGHPYLGAAVLTLMALAGSTQVATGRSALEPGERTVAHR
                     AGGVPVRSSHLPRAVMSRTGTTSGEPTLGVTKNGHIILSALQQTGAEVLKSTDEGRSW
                     EVISPQLAESIAVHQVSLDPYVYVDDKNEDVQRIFTIDLTVACSYMSFSDDGGESWTT
                     NPLACGRPVNDHQTLFAGPPASSPTVGYPNIVYYCWNDVATSSCSRSLDGGITFHPTG
                     SPAFIGYETEGEASGHCGGIHGHGVVDDEGIVYLPREFCARPFLAISRDEGTTWERVR
                     VSSLRAIRNGDPSVDVDSAGNIYYAWVGPKRLPFLAVSKDGGKSWSKPMMIGAPGVHE
                     VNLLTLDVGAPGRVAFAYMGSKDAEHPKTWDGFMGMSVTALKASPIFYSAAVSRDEDP
                     LKRGACGPARCGETILDFIDVVIAPDGSPWASFVDACDAKCIETEFESGNEGAVGHLV
                     GGPALDR"
     gene            complement(1531..1992)
                     /locus_tag="VNP73_11815"
     CDS             complement(1531..1992)
                     /locus_tag="VNP73_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66653.1"
                     /translation="MKRRTVYPLLVMVLLTGTLQAPVRAGMPKTRVVTTEYATPSASV
                     VAAGGGVHACAPAAGGGTANIGCVDIPTKTTERFVLIEAADLTTLPIPMWVGQEGVNT
                     GGYFCGGTGEPIPIAPGVPITIWAFPYTVDPLCQGVSTHGTLTLTFSSGKK"
     gene            complement(2063..2491)
                     /locus_tag="VNP73_11820"
     CDS             complement(2063..2491)
                     /locus_tag="VNP73_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL66654.1"
                     /translation="MLITRASRRETSDLEEFFKEEEWDDPVLDKGIAFIARAGKIIGN
                     VRLIEVDTNVLVIEDILVSKDRRGDRIGTQLVQAAMNSRGGKLYLACHDERLNWYGRL
                     GFTVVDFDALPEPVKTFYEETKAAPHQLPEGHVHHFMTAR"
     gene            complement(2494..3387)
                     /locus_tag="VNP73_11825"
     CDS             complement(2494..3387)
                     /locus_tag="VNP73_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWL66655.1"
                     /translation="MIDLHTHTTVSDGGDSPSELVSKAHAAGLSAIAVTDHDNDSGCA
                     EAVSKGNELGVEVVRGVEISCDVDDLTERGFTPSARPTMHLLGYFIPPVENPLSSALA
                     ELQYHRANRNKIIVDRLNELGIPVTFEEVENEAGGPGAQIGRPHFAAVLVRHGAVPDY
                     QTAFDDYLAKGAKAYIGRKLYKPAEATELMLSAGVVPVLAHPFTLNLSFEDLERFVDA
                     LVEAGLKGIEGYHGDMPEVEQEPFRALGRAKDLIVSGGSDYHGDMRPDRGMPGGKHNV
                     ICPDEVLEELRSAATSAAASN"
     gene            complement(3384..3929)
                     /gene="cysC"
                     /locus_tag="VNP73_11830"
     CDS             complement(3384..3929)
                     /gene="cysC"
                     /locus_tag="VNP73_11830"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWL66656.1"
                     /translation="MKQANRERGSVIWFTGLSGSGKTTIAHVVEEKLLDAGVPVEILD
                     GDVVRENLSKGLGFSEEDRNTNIRRIAFVAHLLQRNGVFVITAAISPYKAIRDEARAM
                     IKDFVEVYADAPLEVCEERDVKGLYAKARAGEIKGFTGIDDPYEAPDNAEVVAKTGEL
                     SVEESAQKVIDKLIELKYIEA"
     gene            complement(3940..5400)
                     /locus_tag="VNP73_11835"
     CDS             complement(3940..5400)
                     /locus_tag="VNP73_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWL66657.1"
                     /translation="MGFLKKLTGKGEESAVAGKPGRKVMVLGLDCAPPEHIFDEYAGD
                     IPNLTKLKENGVFGPLESITPPITVPAWACMMTSKDPGTLGIYGFRNRKDHTYDGLEF
                     ATSFKIKEPTVWDILSENGKDCIVMSVPPMYPPKPTNGIQIGCFLTPSNDADYTYPKE
                     LKAEIESNVGEYIFDIRNFRTDNTQYIIDEAYKMTDMKWKTADYLLSNKPWDFFMVVE
                     MGPDRLNHGIWSFIDPNHPRYEPDNPYKESLREYYRYLDGKIGDLLAKHADDDTVVLV
                     VSDHGAKAMVGGVCFNEWLQQEGYLAFTGAGAGGGPTEITPINKMEIDWSRTKAWGDG
                     GYYGRLFLNVAGREPNGIIPQDQYETVRDELIGKIEAMVDHEGNPMGNKAHKPEELYG
                     TTNGVAPDLIVIFGELTWRSVGSLGHGSIYTFENDTGPDEANHAEHGIFIMSNAPGHA
                     TGEKQGLHLWDVHSTILDLFDLPPAQGALGTSALKK"
     gene            complement(5403..6587)
                     /gene="sat"
                     /locus_tag="VNP73_11840"
     CDS             complement(5403..6587)
                     /gene="sat"
                     /locus_tag="VNP73_11840"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HWL66658.1"
                     /translation="MPSTLKDVPGSIAPHGGALEIRMASEDEVADLKKKAAGLPTIEA
                     TSRRILSDLELLATGAVSPNRGFMTKADYTSVVNDMHLANGLPWSLPITLAPSEAELA
                     NLKEGSAAAITYEGRPVAIIDIEEIFDYDPKDEAIKCFKTDDADGHPGVAHIFSQGPK
                     YVGGDITVLENVFAHEFSEYRNTPMKLRQEIADRGWKTVVAFQTRNPIHRAHEYLTKV
                     ALEGVDGLIIHPLVGGTKGDDIPAEVRMKCYEILMENYYPHDRVVLSVFPAAMRYGGP
                     REAIWHALLRKNYGVTHFIVGRDHAGVGDYYGTYEAQEMFDKFADGELGISPLKFEHT
                     FFCKKCEGMASAKTCPHDRENHVHLSGTQVREMLSNGQEPPHEFSRPEVARILIEAQR
                     SK"
     gene            complement(6736..7449)
                     /locus_tag="VNP73_11845"
     CDS             complement(6736..7449)
                     /locus_tag="VNP73_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66659.1"
                     /translation="MPPTEIDELENMIKQIPGVLGCAILRSADGKPVEIQAFTQTGVD
                     RDNIHSMILEEVSSRALDASMNHIYVFELEAESYFGDLESLERAVEFAEQDARTRGAM
                     SEEERATKPPSKPGDRPRVTKVALSSSSWTSEATVSLGDVIGQASSEKTPHGLKVVAE
                     ATLAAASKVVHDVTFELKSASLVNVSGEEAVLVLVDEDGTNMLGAALTRGGPISEATV
                     RATLDAINRRLALAQAAEE"
     gene            7928..>8853
                     /locus_tag="VNP73_11850"
     CDS             7928..>8853
                     /locus_tag="VNP73_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWL66660.1"
                     /translation="MAKSGRVWIAVTILATLVVPVASQAGERERLEEIRQQRAKLHEQ
                     IEVHEAEADSIQAKAKALNETMILLRRDLAKLDADITEIESEVRTAQARIDETQGEID
                     KVEGVATKQAVSLYKAGATETIDALLGAKSLTELDSRIEFLGVAAQKNTDALIEYHRL
                     QLEIQDEHAQLFATKQDLEATRTEQARIYARLDKSYTEHKAQLAKLEQILGHEHAEEG
                     NLLIAEKRIEGDILAAQAVESSLARGVSREGFIWPLNGAINSYYGPRWGRMHTGLDID
                     GTTGQPIVSSKEGTVIMASSYSGYGNTVIVDHG"
BASE COUNT         1625 a         2738 c         2755 g         1735 t
ORIGIN      
        1 gatcgatcta cgacgtcgtt gcacaagctc ggaccacggc ccgactgata gcggccgggc
       61 agagcgaacg ctggcctaac tgcgcttaaa gaacttcccc catcccccac tgtcgggtgg
      121 accggggtcc cgaaaggggc cccggtcgct cgtcgttgtt ctatctatct aaggctgggc
      181 cccccacgag gtggccgacg gcgccttcgt tgccgctctc gaactccgtc tctatgcatt
      241 tagcgtcgca ggcgtccacg aaggaagccc acggcgatcc gtccggagcg atgacgacgt
      301 ctatgaaatc aaggatggtc tcgccgcagc gcgccggtcc gcacgcaccc cgcttgagcg
      361 gatcttcatc ccggctaact gcagccgagt aaaagatggg tgacgcctta agcgcggtca
      421 cgctcatacc catgaagcca tcccacgtct tgggatgctc cgcgtccttg ctgcccatgt
      481 aggcgaacgc gaccctgccc ggtgccccga cgtcgagcgt aaggagattc acttcgtgaa
      541 cccccggcgc gccgatcatc atcggcttgc tccacgactt gccgccgtcc ttcgaaaccg
      601 ctaggaacgg caaccgtttg gggccgaccc atgcgtaata gatgttgccc gcggaatcga
      661 cgtcgaccga aggatcgccg ttcctaatgg cccggagaga tgacacccgc actcgctccc
      721 aagtcgtccc ttcgtctctg ctgatcgcca ggaaggggcg cgcgcagaac tcacgcggta
      781 ggtacacgat cccttcatcg tcgaccactc cgtggccatg gatcccacca cagtgaccgc
      841 tcgcctcgcc ctccgtctcg tatccgatga aggccggcga gccggtgggg tggaatgtga
      901 ttccaccgtc gagcgaacgg gagcacgagg aagtagcgac gtcattccag cagtagtaga
      961 cgatgttcgg ataaccgaca gttggggaac tggcgggagg acctgcgaac aaggtttggt
     1021 gatcgttcac cggacgtccg caggcaagtg gattcgtggt ccacgactca ccgccgtcgt
     1081 cgctgaacga catgtagctg caggcaacgg ttagatcgat cgtaaagatc cgttgtacgt
     1141 cctcgttctt gtcgtcgacg tagacgtacg gatcgaggct gacctggtgt acggcgatcg
     1201 actcggccag ttgaggtgag atgacttccc agctgcgacc ctcgtcggtg gacttgagta
     1261 cctcggcgcc ggtttgttga agcgccgaga gaatgatgtg cccgttcttg gtaacaccga
     1321 gtgtgggttc acccgaagtg gttcccgttc gcgacatgac ggctctcggc aggtgcgaag
     1381 acctcaccgg taccccaccg gctcggtgag ccacggtccg ctcgcccggc tctaaggcag
     1441 accgtccggt agcaacctgt gtcgatcccg caagggccat gagtgtcagg acggccgcgc
     1501 cgagataagg atgcccgagc ttcaaaccga tcacttcttg ccggacgaga aggtgagcgt
     1561 cagcgttccg tgggtggaca ctccttggca aagggggtcc accgtgtagg gaaaagccca
     1621 gatcgtgatc ggaacccccg gcgcgatcgg gatcggttcg cccgttccac cgcagaagta
     1681 ccccccggta ttgacgccct cctgtccgac ccacatcggg atcggcaagg tcgtcaagtc
     1741 cgcggcttcg atcaacacga agcgttcagt cgtcttggtt gggatgtcga cacatccgat
     1801 gtttgcggta ccgcctcccg ctgcgggggc acacgcatgg acacctcctc cggcggccac
     1861 gacggatgct gacggcgttg cgtattcggt cgtgacgacg cgggtcttgg gcataccggc
     1921 ccgcacgggg gcttgcagcg tgcctgttag aagaaccatc accagcaacg ggtaaaccgt
     1981 gcgacgcttc atctctcatc tccttcgatc gcgtctttcg aaggggatta cttagttagg
     2041 cgaaagtggt aacagcgtcc gctcagcgtg cagtcatgaa gtggtgtaca tgcccctcgg
     2101 ggagttggtg gggagccgct ttcgtctctt cgtagaacgt cttgaccggc tcgggtaggg
     2161 cgtcgaaatc gaccacggtg aagccgagcc tgccgtacca gttcaagcgc tcgtcgtggc
     2221 acgcgaggta gagcttcccg ccgcggctgt tcatcgcagc ttgcaccagt tgtgttccga
     2281 tccggtcgcc ccgccggtcc ttcgacacca agatgtcctc gatcactaga acgttcgtgt
     2341 cgacctcgat cagtcgcacg ttgccgataa tcttcccggc ccgtgcgatg aacgcgatgc
     2401 ccttatcgag gaccgggtcg tcccattcct cttccttgaa gaactcctcg agatcggagg
     2461 tctcgcgacg tgatgcgcgg gtgatcaaca tcgtcaatta ctagcagccg cggaggtcgc
     2521 cgccgaacgg agctcctcga ggacctcgtc gggacagatc acgttgtgct taccccccgg
     2581 catgccccgg tccggccgca tgtcaccgtg gtagtcgctc cccccggaca cgatcaggtc
     2641 cttggctcgg cccagggcac ggaagggctc ctgctccacc tcgggcatgt ccccgtgata
     2701 cccctcgatg ccttttagcc cggcctcgac gagtgcatcg acgaagcgct cgaggtcctc
     2761 gaaggaaaga ttcagagtga atggatgggc gaggacgggg acaacaccgg ccgacagcat
     2821 cagctcggtg gcttccgccg gcttgtagag cttgcggccg atgtaagcct tcgctccttt
     2881 ggcgaggtag tcgtcgaacg ccgtctggta gtcgggaacg gcgccgtgtc gaacgagcac
     2941 ggctgcgaag tgtggccggc cgatctgtgc gcccggcccg cccgcctcgt tctccacctc
     3001 ttcgaacgtg acggggatac caagctcgtt caatcgatcg acaatgattt tgttgcggtt
     3061 ggcgcggtgg tactgcaatt ccgccagcgc agacgagagc ggattttcga cgggtggaat
     3121 gaagtatccg agcagatgca tcgtgggccg ggccgacggc gtgaagccac gctcggtgag
     3181 gtcgtcgaca tcgcacgaga tctcgacacc gcgaacgacc tcgacaccga gctcgttgcc
     3241 cttggacacc gcttccgcgc aaccggagtc gttgtcgtgg tcggtaaccg cgatcgcgct
     3301 gagcccggcc gcatgagcct tggaaacaag ttcggacggg gagtctcccc cgtccgaaac
     3361 agttgtgtgc gtgtgtaggt cgatcaagct tcgatgtact tgagctcgat gagcttgtcg
     3421 atcaccttct gagccgactc ttcgacactc agctcgccgg tcttggcaac tacctctgca
     3481 ttgtcgggag cctcgtacgg gtcgtcgatg ccggtgaagc ccttgatctc gccggcacga
     3541 gccttggcgt acaaaccctt tacgtcacgc tcctcgcaca cctcgagcgg agcatcggcg
     3601 tagacctcga cgaagtcctt gatcatggcg cgggcctcgt cgcggatcgc cttgtaaggc
     3661 gagatcgcgg cggtgatgac aaacacgccg ttgcgctgca gaaggtgagc gacgaatgcg
     3721 atgcggcgga tgttcgtgtt gcgatcctcc tcggagaagc cgaggccttt cgaaaggttc
     3781 tcccgaacga cgtcgccgtc caggatctcg acgggcacgc cggcgtcgag gagtttctct
     3841 tcgacgacat gggcgatcgt ggtcttgccc gagcccgaaa gccccgtgaa ccagataact
     3901 gagccccgtt ctctgttcgc ttgcttcatg cattgcctcc tatttcttga gagctgatgt
     3961 tcccaacgca ccttgggcag gcggcagatc gaacagatcc aggatcgtcg agtgcacgtc
     4021 ccacaggtgt aaaccttgct tctcaccggt cgcgtgtccc ggcgcgttgc tcatgatgaa
     4081 gatgccgtgc tcggcgtggt tcgcttcgtc cgggccggtg tcgttctcga acgtgtagat
     4141 cgagccgtgg ccgagtgaac cgaccgagcg ccatgtgagc tccccgaaga tcacgatgag
     4201 gtcgggtgct acaccgttcg tggtcccgta gagctcttca ggcttgtgcg ccttgttgcc
     4261 catcggattg ccttcatggt ccaccatcgc ttcgatcttg ccgatcaact cgtcacgcac
     4321 ggtctcgtac tgatcctgcg ggatgatccc gttcggctcg cgtccggcga cgtttaggaa
     4381 caagcgaccg tagtaaccgc cgtcgcccca cgccttcgtc cgagaccagt cgatctccat
     4441 cttgttgatc ggagtgatct ctgttggtcc cccgcccgcc ccggctccag tgaatgcgag
     4501 gtaaccctct tgctgcaacc attcgttgaa gcacacgcca ccgaccattg ccttggcgcc
     4561 gtggtccgac accacgagca cgaccgtgtc gtcgtcggcg tgcttcgcga ggagatcgcc
     4621 gatcttgccg tcgaggtagc ggtagtactc gcgcagcgac tccttgtaag gattgtcggg
     4681 ctcgtagcgc gggtggttcg ggtcgatgaa agaccagatc ccgtggttca gacggtctgg
     4741 acccatctcg acgaccatga agaagtccca tggcttgttc gacaacaggt agtcggccgt
     4801 cttccacttc atatcggtca tcttgtaggc ctcgtcgatg atgtactgcg tgttgtccgt
     4861 gcggaaattg cggatgtcga agatgtactc gccgacgttc gattcgatct cggccttcag
     4921 ttccttggga taggtgtaat ccgcgtcgtt cgaaggcgtg aggaagcacc cgatctggat
     4981 cccattcgtc ggcttgggtg gatacatcgg cgggaccgac atcacgatgc agtccttgcc
     5041 gttctcgctc aggatgtccc acacggtcgg ttccttgatc ttgaagctcg ttgcgaactc
     5101 cagaccgtcg taggtgtggt ccttgcggtt gcggaaaccg tagataccga gcgtgccggg
     5161 gtccttcgac gtcatcatgc aggcccatgc cggaaccgtg atcggcgggg tgatcgactc
     5221 gaggggaccg aaaacaccgt tctccttgag tttggtgagg ttcgggatgt cgcccgcgta
     5281 ctcgtcgaag atgtgctccg gcggcgcgca gtcgagcccg aggaccatga ccttgcggcc
     5341 aggcttcccg gctacggccg actcttcgcc tttaccggtc agctttttca ggaaacccat
     5401 ggttacttgc tcctctgtgc ctcgatcagg atgcgtgcga cctcggggcg agagaactcg
     5461 tgaggcggct cttggccgtt cgacagcatc tcgcgcacct gggtgcccga gaggtgcacg
     5521 tggttctcgc ggtcgtgggg gcatgtcttg gcagatgcca tgccctcgca cttcttgcag
     5581 aagaacgtgt gctcgaactt gagcggtgag atgccgagct cgccgtcggc gaacttgtcg
     5641 aacatctcct gcgcctcgta ggtgccgtaa tagtcgccga cgcccgcgtg gtcgcggccc
     5701 acgatgaagt gggtgacgcc gtagttcttg cgcagcagcg cgtgccagat ggcctcgcgc
     5761 ggcccgccgt aacgcatcgc cgccggaaac accgacagga cgacacggtc gtgcgggtag
     5821 tagttctcca tgaggatctc gtagcacttc atccgcacct cggcagggat gtcgtcgcct
     5881 ttggtgccac caacgagcgg gtggatgatc aaaccgtcga cgccctcgag cgcgaccttt
     5941 gtgaggtact cgtgcgcgcg gtggatcggg ttgcgggtct ggaacgcaac tacggtcttc
     6001 caaccgcggt ccgcgatctc ctgacgaagc ttcatcggag tgttgcggta ctcggagaac
     6061 tcgtgtgcga agacgttctc tagaacggtg atgtcaccgc cgacgtactt cggtccctga
     6121 ctgaagatgt gagcgacacc cgggtgtccg tcggcgtcgt cggtcttgaa gcacttgatc
     6181 gcctcgtcct tggggtcgta atcgaagatc tcctcaatgt cgatgatcgc taccggacga
     6241 ccttcgtagg tgatcgcggc cgccgaacct tccttcaggt tcgccagctc ggcttcgctc
     6301 ggcgccaaag tgatgggaag gctccaggga agtccgttcg ccaggtgcat gtcgttgacg
     6361 accgaggtgt agtcggcctt cgtcatgaaa ccgcggttcg gagacacggc tccggtcgcc
     6421 agaagctcga ggtccgaaag gatccggcgg gacgtcgcct cgatcgtcgg cagaccggct
     6481 gctttcttct tgaggtcggc cacttcgtcc tccgaggcca tgcggatctc gagcgcgccg
     6541 ccgtggggcg cgatgctgcc gggaacgtcc ttcaatgtgc tgggcatgtt tgaccactcc
     6601 tttttcggag acgaaggggg cggaggtgct tgctggaaga ccctccaggc acactcctat
     6661 agcactctaa cgttaacagt tcgccgccga tggggccagt tcctatgccc cggggcgggt
     6721 cggggacgaa agggtctatt cctccgcggc ctgggccaga gcgagccggc ggttgatcgc
     6781 atccagggtc gccctgacgg tcgcttcgct gatagggccc ccccgggtga gggccgcgcc
     6841 gagcatgttc gtcccgtcct cgtccacgag caccaaaacg gcctcctcac cgctcacgtt
     6901 caccagtgag gcactcttga gctcgaaggt cacgtcatgg acgaccttcg aagccgcggc
     6961 cagggtggct tcagcaacga ccttgaggcc gtgaggggtt ttctcggaag aggcctgtcc
     7021 gatgacgtct cccaacgaca ccgtggcctc ggaggtccac gacgacgatg acagggccac
     7081 cttggtcacc ctgggacggt ctccgggctt agaggggggt ttcgtggccc tctcctcctc
     7141 ggacatcgcc cccctggtgc gagcgtcctg ctcggcgaac tcgaccgccc tctcgagcga
     7201 ctcgaggtcc ccgaaatagg actcggcttc gagctcgaag acgtagatgt ggttcatgga
     7261 ggcatcgagg gccctggagg aaacctcttc caggatcatc gaatggatgt tgtcccggtc
     7321 gacgccggtc tgggtgaagg cctggatctc gaccggtttg ccgtcggcgc tccgcaggat
     7381 cgcgcagccc agaacgcctg ggatctgctt gatcatgttt tcgagttcgt cgatctccgt
     7441 agggggcata ttttcctccg ccgacgacct agctcgtcgg cgctcaactc ggcaggtgga
     7501 ggcccaagca taggagggcc gccagagcct cgcaatagac agaacaaggg ctcggccccc
     7561 gggctggcaa aggaggaaag agaaacgccg atggccatcg gcggaataga aaatgcaccc
     7621 gccgagcctt cgtcccgagc aagcccggtt tacaactcaa cgggtgcagg cgcactctaa
     7681 agggcgttgt gggtgggaca caacccctaa aagcgccttt tcttcgcgac ccacaaaaga
     7741 agtttccccc tctcgggggg tcgagtagga gggatccgag cccccggcga gaaccgttgg
     7801 agagttgaga gccgaagggc cctatggcag gatgccgggc ggcatcaggc cggcccggcg
     7861 ggcgcaggac cgggctctcg aggggcctcg cgggcccacg gacagacccc taaagaggga
     7921 gagctgcatg gcgaagagcg gccgcgtgtg gatcgcagta acgatcctcg cgacccttgt
     7981 cgtccccgtc gcttctcagg ccggcgagcg tgagcgcctc gaagagatcc gtcagcagcg
     8041 agccaagctc cacgagcaga tcgaggtcca cgaagccgaa gccgactcca tccaggcgaa
     8101 ggcgaaagct ctaaacgaaa cgatgatcct gctccgccgc gacctcgcga agctggacgc
     8161 agacatcacc gagatcgagt ccgaggttcg caccgcgcag gctcggatcg acgagacgca
     8221 gggtgagatc gacaaggtcg agggagtcgc gacgaagcaa gcagtctctt tgtacaaggc
     8281 cggcgcgacc gagacgatcg acgcgctatt gggggcaaag tccctgaccg agctggactc
     8341 ccgcatcgaa ttcctagggg ttgccgcaca gaagaacacc gatgcgctga tcgagtacca
     8401 ccgactccag ctcgagatcc aagacgagca tgcacaactg ttcgcaacca agcaggacct
     8461 cgaagcgacg cggacggagc aggctcgcat ctacgcccgg ctcgacaaga gctacaccga
     8521 gcacaaggcg cagctggcga agctcgagca gatcctcggc cacgaacacg ccgaagaggg
     8581 caacctgctc atcgccgaga agaggatcga aggcgacatc ctcgccgcac aagctgttga
     8641 gtcctccttg gcgcggggcg tctcgcgcga aggtttcatc tggccgctga acggcgcgat
     8701 caactcttat tacggcccgc gttggggccg catgcacacg ggtctcgaca tcgacggaac
     8761 gaccggccag ccgatcgtgt catcgaaaga gggaaccgtg atcatggcgt cgtcgtactc
     8821 aggttacgga aacaccgtga tcgtcgatca cgg
//
LOCUS       DATMED010000453         3649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1513332, whole genome shotgun sequence.
ACCESSION   DATMED010000453 DATMED010000000
VERSION     DATMED010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513332"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VNP73_11855"
     CDS             <1..391
                     /locus_tag="VNP73_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWL66661.1"
                     /translation="SRRVTFERYADVWTKLSDVFIGHPDVGYAIMSEPVALKKKGDLS
                     PTEVWEAAAQTAVDAIRSNGDNKTILVNGYRWSSLANWPEIHPDPWIVDPANNFFYTA
                     HHYWDRDASGSYDYSYADEVAYAERNE"
     gene            complement(399..920)
                     /locus_tag="VNP73_11860"
     CDS             complement(399..920)
                     /locus_tag="VNP73_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66662.1"
                     /translation="MDKVIAFFLLCVAAFALVACSNQEPPPTSPIGETPSAVLSDTPE
                     PEPDTASERERFITKADRMCTRILNKLRLLPQPNDLRSAATFTRKANRLNEVMLNRLR
                     ALTPPRFRKALSDDYAEAERLIALTSGEVIAALKSGDPARYQVIALKIQKATRKLNGS
                     LRRFGFDACADSS"
     gene            complement(932..1465)
                     /locus_tag="VNP73_11865"
     CDS             complement(932..1465)
                     /locus_tag="VNP73_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWL66663.1"
                     /translation="MKRISVVGSSGSGKTTFASRLARALSLPHIELDAIYHRSGWTPL
                     PRHEFRAEIERQVGEAGWVIDGNYGTEVLDLVWVNADTVVWLQIPRHLVMRRIIGRSL
                     RRAFKREELWNGNRERFRNLLSLDPERSVILWAYTRYDKYREQYEQAMEDPTWSRLRF
                     IRLTSQGEVRDFLDSCR"
     gene            complement(1466..1824)
                     /gene="ssrA"
                     /locus_tag="VNP73_11870"
     tmRNA           complement(1466..1824)
                     /gene="ssrA"
                     /locus_tag="VNP73_11870"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1887..2246)
                     /locus_tag="VNP73_11875"
     CDS             complement(1887..2246)
                     /locus_tag="VNP73_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWL66664.1"
                     /translation="MNETLAAVEHFNEAFNSHDVDRIMSLMTDDVVFESTSPAPDGQR
                     FEGQAAVSAVWQELFSSTPDAYFESEEIFVGDDRAAIRWIYTFTDSNGSKQHVRGVDV
                     IHVRNGKVAEKLSYVKG"
     gene            complement(2256..2729)
                     /gene="smpB"
                     /locus_tag="VNP73_11880"
     CDS             complement(2256..2729)
                     /gene="smpB"
                     /locus_tag="VNP73_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWL66665.1"
                     /translation="MAGPPDKPGTKLIAQNRKARHNYEIEESVEAGLVLTGTEVKSCR
                     AGKANLADSYAAIRDGEAWLMQCHISPYSHGNKQNHDPVRPRKLLLHRGEIERLGAKV
                     SQEGRTLVPLRLYFKHGLAKAEIAVGRGKKTYDKRATIAKKEAERSMQRELGRRR"
     gene            complement(2729..>3649)
                     /locus_tag="VNP73_11885"
     CDS             complement(2729..>3649)
                     /locus_tag="VNP73_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWL66666.1"
                     /translation="YALFYSPKGFETFQELTTGRFSGIGVWLKQKSNDLEIISVLPSS
                     PALEAGLRQGDAIRTIDGLQVSKMTTDEAVARIKGPEGSEVDMGVERDGTTLTFSIVR
                     ARIEIPNVRASATDGNLGYIRLLGFSRGAGKQVRSEVKRLTDEGAEGIVLDMRDNGGG
                     LFAEAVDVASVFIEQGPIVTYRETAQEDVVYKAEGQAYEEVPLVVLVNEGTASASEIV
                     AGALQDSERAVVVGTTTFGKGSVQEVVPLLDASAVKLTTAAYLTPEGHNINGKGIDPD
                     VQVQAEPVVQRQRAVEILKGIVLSVNETQG"
BASE COUNT          674 a         1138 c         1083 g          754 t
ORIGIN      
        1 gtcgcgccga gtcaccttcg agcgctacgc cgacgtttgg acgaagctca gcgacgtctt
       61 cataggacac ccggatgtcg gttacgccat catgtcggag cccgtcgctc tcaagaagaa
      121 gggtgacttg tctccgaccg aggtgtggga agccgcggcc cagacggcgg tcgatgcgat
      181 ccgctcaaac ggtgacaaca agacgatcct cgtaaatggt taccggtggt cgtcactcgc
      241 caactggccg gagatccatc ccgatccttg gatcgtcgat cctgcgaaca acttcttcta
      301 cacggcccac cattactggg accgcgacgc gtcgggcagc tacgactact cctacgccga
      361 cgaggtcgcc tacgccgaac gcaacgagtg agccgcgatc aagaggagtc ggcgcaagca
      421 tcgaaaccga acctccgtaa actcccgttg agcttcctag tcgctttctg gatcttgagt
      481 gcgatcactt gatagcgggc cgggtcgcca ctcttgagcg cggcgatgac ttctcccgag
      541 gtaagtgcga tcaacctctc ggcctctgca tagtcgtcac tcagggcttt gcggaagcga
      601 gggggcgtaa gtgcccgaag tcgattgagc atcacctcat tcaggcggtt ggccttcctc
      661 gtgaaggtgg cggcggatcg caggtcgttc ggttgaggga gcagacgaag cttgttcagt
      721 atccgtgtgc acatcctgtc ggccttagtt atgaaccgct cccgctccga tgccgtgtcg
      781 ggttcgggct ccggagtgtc gctaagaacc gcgctcggag tctctccgat cggagacgtt
      841 gggggcggtt cctggttgga gcatgcgacg agtgcgaatg ccgcgacgca caggagaaag
      901 aaagcgatca ccttatccat gcctcctcag gttagcgaca agaatcaagg aaatctcgca
      961 cctcgccctg cgaggtaagg cgaatgaaac gaagccggga ccaggtcgga tcctccatcg
     1021 cttgctcgta ctgctctcgg tacttgtcgt agcgggtgta tgcccagagg atcacggatc
     1081 gctcgggatc aagtgacaga aggttcctaa agcgctcccg atttccgttc caaagttcct
     1141 cgcgtttgaa ggctcgacgg agagagcgtc cgatgatcct tcgcatgacc aggtgacgag
     1201 gtatctgtag ccacacgacg gtgtcggcgt tgacccagac tagatccaga acttccgtcc
     1261 catagttccc gtcgatcacc cagcccgctt ctccgacctg gcgttcgatc tcggcgcgga
     1321 attcatgtcg tgggagcggt gtccatccgg accggtgata gatcgcgtcg agttcgatgt
     1381 gtggcaaaga aagcgcgcgc gctagccggg aggcaaacgt ggtcttgccg gagccagaac
     1441 tgccgacaac agagatgcgc ttcattagtg gagctgccgg gaatcgaacc cgggtcctcc
     1501 aaccccgtag tagggcttct acgagcgtag ttcgtgatca agattcggcc ccggccggct
     1561 cacgaacggc actggtcggg gtctagcccg agctggatgt tccggcggtt cgcctcgggc
     1621 gaactcccgc cggtaagcct gctaggcgac gccggatcct gaggtcgcag gcaggcctca
     1681 ggccgacggc catcacttaa gtggatggca actgtgcatt aagcagcgac agttaattaa
     1741 gtccgagcta tttaacgagg catctcggaa cctcggctcg cttcccctac cccggttgat
     1801 cagagtcgat acctgttcag ccccaaggta ggacagattg tcaatgtcct acctaagggt
     1861 aacgccgggg accggcacga ccttacctag cccttgacgt acgagagctt ctcggccacc
     1921 ttgccgttgc gaacgtggat cacatcgacg ccgcggacgt gttgcttgct tccgttcgag
     1981 tcggtaaacg tgtagatcca tcggatcgcg gcacggtcgt cgccgacgaa gatctcttcc
     2041 gactcgaagt aggcgtcggg ggtcgagctg aacaactcct gccacaccgc gctgacggca
     2101 gcttggccct cgaaccgctg cccgtcgggt gcgggcgacg tgctttcgaa gaccacgtcg
     2161 tcggtcatga gcgacatgat gcgatcgaca tcgtgggagt tgaaggcctc gttgaaatgc
     2221 tcgaccgctg ccagcgtttc gttcatgtcg agactctagc gccggcggcc aagctctctt
     2281 tgcatagagc gctccgcttc cttcttggcg atagtcgcgc gcttgtcgta ggtcttcttc
     2341 ccccgaccca ctgcgatctc ggctttagcc aagccatgct tgaaatagag gcggagcggg
     2401 accagagtgc ggccctcctg cgacaccttc gcaccgaggc gctcgatctc gccccggtgt
     2461 agcagcagct tccgcgggcg caccgggtcg tggttctgct tgttcccgtg cgagtagggg
     2521 ctgatatggc attgcatcaa ccaggcctcc ccgtccctga tggcggcgta cgaatcggcc
     2581 aggttcgcct tgcctgcacg acacgatttc acttcggttc cggtcaacac cagtccggct
     2641 tcgaccgatt cctcgatctc atagttgtgc cgcgctttcc gattctgcgc gatgagtttg
     2701 gtgccgggct tgtcgggagg gcccgccact agccctgcgt ttcgttaacg gagaggacga
     2761 tccccttgag gatctccacc gcccgctgac gttgcacgac cggctcggct tgaacctgga
     2821 cgtcggggtc tatccctttt ccgttgatgt tgtgtccctc aggagtcagg taggcggccg
     2881 tcgtcagctt gaccgcggac gcgtcgagca agggaacaac ttcctgaacc gatcctttac
     2941 cgaatgtcgt cgtccccacg acgaccgcgc gctcgctgtc ctgaagagca ccggcgacga
     3001 tctcggatgc gctggccgtc ccttcgttca cgaggacgac cagggggacc tcttcgtacg
     3061 cctggccctc cgctttgtag acgacgtctt cctgcgccgt ctcgcgatag gtgacgatcg
     3121 gaccctgctc gatgaagacg ctcgcaacat cgaccgcctc tgcgaagagg ccgccgccgt
     3181 tgtcccgcat gtccaggacg atcccctcgg ccccctcatc ggtcagtcgc ttcacctccg
     3241 agcgaacctg tttcccggca cccctagaga aacccaatag acggatgtag cccaggttgc
     3301 cgtcggtagc ggacgctctg acgttcggga tctcgatcct ggcccgcact atcgagaacg
     3361 tcagggtggt cccgtcgcgc tcgaccccca tatcaacctc gctgccctcg ggtcctttga
     3421 tacgagccac ggcctcgtcg gtggtcatct tcgaaacctg cagtccatcg atcgtgcgga
     3481 tcgcatcgcc ctgccgcagg cccgcttcga gagccggaga cgacgggagc acggagatga
     3541 tctcgaggtc attgctcttc tgcttcagcc acaccccgat accggagaag cgaccggtgg
     3601 tcagctcttg gaacgtttcg aatcccttcg gcgaatagaa cagtgcgta
//
LOCUS       DATMED010000454         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5745702, whole genome shotgun sequence.
ACCESSION   DATMED010000454 DATMED010000000
VERSION     DATMED010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5745702"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="hflX"
                     /locus_tag="VNP73_11890"
     CDS             complement(<1..1048)
                     /gene="hflX"
                     /locus_tag="VNP73_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWL66667.1"
                     /translation="MTRRDGHEVRPERERAILVGAEFGSVDAEEAEWSLEELEALADT
                     AGADTLDRMVQKRDSPDPATYLGRGKAKEIVDAANALDADILLFDDELTPAQGRNLEE
                     MAGVNPLAERGLKILDRTGLILDIFAQHARSAEGKLQVELAQLNYRLPRLRGWGDVLS
                     RLGGGIGTRRGPGETALETERRAILRRIQRVKKDLDELERTRRLKRKQRVRSGAPVVA
                     LVGYTNAGKSTLLKALTDAGVLIEDRLFSTLDPTTRRLELPFGRHALLTDTVGFVRKL
                     PHQLIEAFHSTLEEVGEATILLHVVDGGRDIERQIQAVNTVLKGLGVMDRATVIAINK
                     IDLLSEVELDKIRGR"
     gene            complement(1081..>2175)
                     /locus_tag="VNP73_11895"
     CDS             complement(1081..>2175)
                     /locus_tag="VNP73_11895"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HWL66668.1"
                     /translation="YFAELIDEWAPEAHADERLYRLGAAVVEALAEGARDAATHRYPS
                     YTGMPEFREAIATWYEKRFGVNLDPDTEIQPLVGSKEGIFHLPVAFIDEGDVALIPDP
                     GYPVYETGTILAGGAPHLMPLKADNDFLPDYDAIPVEVLERARVLWLNYPSNPTAACT
                     DLDFFNTSVAFAAENDLVLAHDAAYTEITFDGYVAPSALEAADAMEHSIEFHSLSKTF
                     NMTGWRIGWVAGAPKAVEAIKRLKTNIDSGIFDAVQRAGIVALRDAQDYVEENNATYT
                     RRRDLLCEGLASMGIDVEPPKGSIYVWVPVPEGHTSESFTTFLLDECDIVVAPGNGYG
                     PSGEGFIRFSLTLPDDRLEEGVERLRKAVL"
BASE COUNT          378 a          725 c          658 g          414 t
ORIGIN      
        1 aacgtccgcg gatcttgtcg agctcgacct cactgagaag atcgatcttg ttgatcgcaa
       61 tcaccgtcgc cctgtccatc actccgagac ccttgagcac agtattgacg gcctggatct
      121 gccgttcgat atcgcggccc ccgtcgacca cgtggagcaa gatcgtcgct tcgccgactt
      181 cctcgagcgt ggaatggaac gcctcgatca gctggtgcgg gagctttctc acgaacccaa
      241 cggtgtccgt aagcaacgca tggcgcccga agggaagctc cagacgccgc gtcgtcggat
      301 cgagcgtcga gaagagccgg tcctctatga gcactcccgc atcggtcaat gccttcaaca
      361 atgtcgattt cccggcgttc gtgtagccca cgagcgcgac caccggagcc cccgagcgga
      421 cgcgctgctt gcgcttgagc cgcctcgttc gctcgagctc gtcgagatcc ttcttgaccc
      481 tttggatgcg ccgcaggatc gcgcgccgtt cggtttcgag cgcggtctca cccggccccc
      541 gtcttgtccc gatacctcca cctagacgcg agagcacgtc accccaacct cgcagccggg
      601 gcaaccggta gttgagttgc gccagctcga cctgaagctt cccctccgcg gaccgtgcgt
      661 gctgcgcaaa gatgtcgagg atcaaccccg tgcggtcgag gatcttcaga ccccgctcgg
      721 cgagcgggtt gacacctgcc atctcctcga ggtttcgccc ctgcgccgga gtcaactcgt
      781 cgtcgaaaag aagaatgtcg gcgtcgagag cgttcgccgc gtcgacgatc tccttcgcct
      841 taccccgtcc aaggtaggtg gcggggtcgg gagagtcccg cttctgcacc attcggtcga
      901 gcgtgtcggc cccagcagtg tcggcaagcg cttcgagctc ctcgagcgac cactccgctt
      961 cctccgcgtc tacggacccg aattccgcgc cgacgaggat ggctcgctcg cgctcggggc
     1021 ggacctcgtg cccgtctctt cgcgtcatct agctcccccc aagggatccg gcacgaacgg
     1081 ttacagcacc gccttccgca gccgctcgac gccttcctcg agtctgtcgt cgggcagcgt
     1141 cagcgagaac cggatgaaac cctcgcccga aggtccgtaa ccgttgccgg gagcaaccac
     1201 gatgtcgcac tcgtccagca ggaaggtcgt aaacgattcg gaggtgtgtc cttcggggac
     1261 cgggacccaa acatagatcg agcccttcgg cggttcgacg tctatcccca tagacgccag
     1321 tccctcgcac agcagatcgc gtcgccgcgt gtacgtggcg ttgttctcct cgacgtagtc
     1381 ctgagcatct ctgagagcca cgatgcccgc acgctgcacc gcgtcgaaga tcccggagtc
     1441 gatgttggtt tttagtcgct tgatcgcttc cactgccttc ggcgcgccgg cgacccagcc
     1501 gatccgccac cccgtcatgt tgaacgtctt cgagagggag tggaactcga tcgaatgttc
     1561 catggcgtcc gccgcctcga gagcgctcgg cgcgacgtaa ccgtcgaagg tgatctccgt
     1621 gtatgccgca tcgtgcgcaa gcacgagatc gttctcggct gcaaacgcga ccgaggtgtt
     1681 gaagaaatcg aggtccgtgc acgcggctgt cgggttgctc ggatagttca gccagagcac
     1741 gcgagcccgt tcgagaacct cgacggggat ggcgtcgtaa tcggggagga agtcgttgtc
     1801 cgctttcaaa ggcatgaggt gcggcgcgcc accggccagg atggttccgg tttcgtaaac
     1861 cggatagccg ggatcgggaa tcagcgctac gtcaccctcg tcgatgaaag ccacgggcaa
     1921 gtggaagatg ccctctttgg atccaacaag cggctggatc tcggtgtccg ggtcgaggtt
     1981 cacgccaaag cgcttctcgt accaggtggc aatggcctcg cggaactctg gcatcccggt
     2041 gtacgaggga tagcgatgag tggcggcgtc gcgcgcccct tcggccagtg cctcgaccac
     2101 cgccgccccc agccggtata gccgctcatc ggcgtgcgcc tcaggggccc actcgtcgat
     2161 gagctccgcg aagta
//
LOCUS       DATMED010000455         5797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1125769, whole genome shotgun sequence.
ACCESSION   DATMED010000455 DATMED010000000
VERSION     DATMED010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125769"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VNP73_11900"
     CDS             complement(<1..455)
                     /locus_tag="VNP73_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL66669.1"
                     /translation="MIDIRRVTGDDWELVRDVRLKALLDTPDWFWSTHEEEVSRLEAW
                     WRDRIASGAWFVARDGDRPVGIAAGIHAPETAESDRQLISMWVENEARGRGIGRRLVA
                     AVESWARTDGARRLQLEVTDKNETGRRFYESYGFVATGRTEPLPRKPEL"
     gene            692..841
                     /locus_tag="VNP73_11905"
     CDS             692..841
                     /locus_tag="VNP73_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HWL66670.1"
                     /translation="MHELSNERGATAVEYGLMVALIAAIIVAVVGLLGQEIRAAFQTV
                     LDALP"
     gene            913..1332
                     /locus_tag="VNP73_11910"
     CDS             913..1332
                     /locus_tag="VNP73_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HWL66671.1"
                     /translation="MRISSTPMYPPFRNATRLRNERGAAAVEFALIAAVLFVFVFGII
                     EFGRLFSELEVLTSAAREGARAAAVRGTSDEVEQAVADAAVPYEVDAAPIPDKTCDDS
                     TSGQAVTVAWDQNFEFSVGLLPAFNRDIEVRGVFRCE"
     gene            1323..2582
                     /locus_tag="VNP73_11915"
     CDS             1323..2582
                     /locus_tag="VNP73_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWL66672.1"
                     /translation="MRVKRALGNETGATIVIVTVALFALTGMIVLVVDVGGLLTLRRR
                     LVTAADSAALAAAQSCGMNQTDAEDKADDFAESNQADANQTAYEATTCGGPSSGRVEL
                     TYASPKDLVFAPILGAPSSRPVSARAEAIWGPAGGVTPVPIELSIDPAGQIDCAYKDL
                     DTTCDYWHDNSADHDINSSSNWGFMNLNLSGVEPDASCPNAGTNERREWIENTRRAPI
                     VITGDMTLVCADSGHSTSSWYAALEGQVGSVKYFPVNDPAQMILTSGKEKYAIIGFVP
                     LRIEEVLRGNDPAAVGVPGESGGCNGNHSFTPGDVFDLDALGCYTAADSITNLEISRR
                     HTTYVSGTDYVYDPISHEVTWFAPSAPSASISFDWAANGEPGKCGNHGPDPNAVCLVL
                     SWQGVRVAGSLPGNGVDFGLRAVRLDG"
     gene            2592..3308
                     /gene="cpaB"
                     /locus_tag="VNP73_11920"
     CDS             2592..3308
                     /gene="cpaB"
                     /locus_tag="VNP73_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWL66673.1"
                     /translation="MVKSRGLIIAIAFVLAGIATMVVYLYLLGVQRQSEGGEMVSVVV
                     SERDVPAGTSLDDLVEEGALTTRALPRSALVRGAVTNLQSLEGRETSAAIVAGEQLTT
                     ARLRGSTTQLPGGSLGIPNGYAALTLPLEAPRVAGGVIQRSDHITIYGTFQNVASQQG
                     SSPASTVTLVPDVEVLRIASSAEEGGANDTQWITLALKPRDAQKVVFAQEQGSVWMSL
                     LPPNQRGTASRPVNAIQLVR"
     gene            3305..4519
                     /locus_tag="VNP73_11925"
     CDS             3305..4519
                     /locus_tag="VNP73_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWL66674.1"
                     /translation="MNQPVGPRILAIGAPPTFRQDVARCMDASPETVDWLPSVTAAEE
                     FVVVRHMEPDVMVLSPEIKDADALGVADFMAKHSPATAVILVRYTSPNGLLPMAMRAG
                     VRDVVDLSRGEEDLADALSRASDWARGVRSHTAEPEHAGERGKIISIFSSKGGVGKTF
                     LSCNLAAALADKGEGDTALLDLDLKVGDVFSYFGQEPTRPIQDLLSLAERKDPEALVA
                     TGKQLGDNLWAYGVTADPACESMPGETTGKIVRNLRANFAYTVIDASNDYSDQTVAVF
                     DLSDEIWLVTGLDVVGMRHLLIGMDTLTSLGIPHDRFRVVLNRSDSKVGVSLTDVERV
                     LRVQVDAKIPSSELVPQSLNKGKPVYLGEPGSEVARAITEIAETIIGTPQSETAPAAQ
                     QRRGLKLLLGLG"
     gene            complement(4536..4679)
                     /locus_tag="VNP73_11930"
     CDS             complement(4536..4679)
                     /locus_tag="VNP73_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66675.1"
                     /translation="MDHRLQLLVGDGFVVQARPESLDQSGLDSIANLPETIAVLRTGQ
                     AFY"
     gene            4671..>5797
                     /locus_tag="VNP73_11935"
     CDS             4671..>5797
                     /locus_tag="VNP73_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWL66676.1"
                     /translation="MVHDRLRDLLNEEEIPLTTTEKAQITKEIQDSVMGLGPLEELLA
                     DTDVTEIMVNGCETVFVERAGKIHRTNARFVDENQLRRTIDKIVTKVGRRIDESSPYV
                     DARLPDGSRVNAIIPPLAIDGPALTIRRFSADPYGTEELVSFGTFSGPMAEFLEACVR
                     GRMNLLVSGGTGAGKTTTLNVLSSYIPHDERILTIEDAAELKLQQPHVVRLESRPPNI
                     EGVGQVAIRDLVRNALRMRPDRIIVGEVRGGEAIDMLQAMNTGHDGSISTIHSNSPRD
                     VLSRLETMTMMAGMELTVRAIREQIASAIDVIVHQERMKDGTRRITHVSEVVGMEGDI
                     ITLQDIFLFNFKAGVDAQGRVLGSLEATGLRPGFLEKLSDRG"
BASE COUNT         1105 a         1896 c         1776 g         1020 t
ORIGIN      
        1 atgagctcgg gcttccttgg gagcggttcg gttcgaccgg tcgccacgaa cccgtagctc
       61 tcgtagaagc ggcgacccgt ttcgttcttg tccgttacct ctagttgaag acgccgggcc
      121 ccgtcggtac gggcccatga ctcaacggcg gcaaccagcc ggcggccgat gcctcgtccc
      181 cttgcttcgt tctcgaccca catggagatg agttgacgat ccgactcggc cgtctcgggc
      241 gcatggatcc cggccgcgat ccccaccgga cgatctccgt cacgagcgac gaaccatgct
      301 cccgacgcga tgcgatcgcg ccaccatgcc tcgagccgac ttacttcctc ctcgtgcgtc
      361 gaccagaacc agtcgggcgt atcgagtaac gccttgagtc gaacgtcgcg caccagctcc
      421 cagtcgtccc cggtgactcg cctgatatcg atcacgaccg cgatcttctc cgaaaaagta
      481 actagtccta aagaactacg gatgtgctgc cgatgactag tcgcacatga ctagtaaccg
      541 gaactcgacg agcacgcagg cccccggcgg ccgcaccagc ccccttgcgt acgtgctcac
      601 tcccaagaac ctctcccctg atgcgtcttc gagctggtcg aaaggaggtg tgcagatgtt
      661 ccacggtctt tcccgctggg tggtcggcca gatgcacgag ctaagcaacg agcgtggcgc
      721 aaccgccgtc gagtacggac taatggttgc cctcatcgcc gcgatcatcg tcgcggtggt
      781 cggacttctg ggtcaagaga tcagagccgc gttccagacg gttctcgacg ctctcccgta
      841 aaccactgcc aaccgactgc gccggccctc acctcccgtc gtccaggtgg gggccggtct
      901 cggttgaaga ccgtgaggat ctcttcgaca ccgatgtatc ccccctttag aaacgcaacc
      961 cgtctccgta acgaacgtgg agcagctgcc gtcgagttcg cactgatcgc cgccgttctg
     1021 ttcgttttcg tcttcggcat catcgagttc ggtcgtctct tcagcgagct cgaggtcctg
     1081 acctcggctg cgcgcgaagg agcccgggcc gctgccgtcc gcggcacgag cgacgaagtc
     1141 gagcaggcgg tcgcagacgc ggccgttccc tacgaagtgg acgcggcccc catccccgac
     1201 aagacctgcg acgactcaac ctcgggtcaa gcggtcaccg tggcgtggga tcagaacttc
     1261 gagttttcgg ttggcctgct ccccgctttc aacagagaca tcgaagtgcg gggtgtcttt
     1321 agatgcgagt gaagcgcgcc cttggcaacg agaccggagc gacgatcgtg atcgttacgg
     1381 tggcgctgtt cgctctcacc gggatgatcg ttctcgtcgt cgatgtcggc ggtctgctga
     1441 cgttgcgccg ccgcctcgtc accgcagccg actccgccgc cctcgccgcc gcccagtcct
     1501 gcgggatgaa ccagacggac gcggaggaca aggccgacga cttcgcagag tcgaaccagg
     1561 ccgacgccaa ccaaaccgcc tatgaggcaa cgacgtgcgg gggcccatcc tccggacggg
     1621 tcgaactgac ctatgcgtcg ccgaaagacc tcgtcttcgc gccgatcctc ggcgcgccgt
     1681 catcgcggcc cgtgtccgca cgagcggaag cgatctgggg acccgccggt ggagtaactc
     1741 ccgttccgat cgagctcagc atcgatcccg cgggtcagat cgactgcgcc tacaaagacc
     1801 tcgacaccac gtgcgactac tggcacgaca actcggccga ccacgacatc aacagcagta
     1861 gcaattgggg attcatgaac ctcaacctct cgggagtcga gccggacgcg tcctgcccga
     1921 acgccggtac gaacgaacgg cgcgaatgga tcgagaacac gcgtcgggct ccgatagtga
     1981 taaccggcga catgacgctg gtatgcgccg acagcggtca ttcgacctcc agctggtacg
     2041 cagcgctcga ggggcaggtc ggcagcgtga agtacttccc ggtaaacgac cccgcgcaga
     2101 tgatcctgac gtccggaaaa gagaaatacg ccatcatcgg cttcgtcccg ctgcgcatcg
     2161 aagaggtgtt acgcggtaac gaccctgccg cggtcggcgt tccgggagag agcggcggtt
     2221 gtaacgggaa tcattcattc acccccggcg acgtcttcga cctcgacgcg ctcgggtgct
     2281 acacggccgc cgactcgata acgaacctgg agatctctag acgccacacc acctacgtct
     2341 cgggaaccga ctacgtctac gacccgatct cgcacgaagt gacttggttc gccccctcgg
     2401 cccccagcgc gtcgatctcg ttcgactggg ccgcgaacgg cgagcccggt aagtgcggca
     2461 atcacggccc cgatcccaat gcggtgtgtc tcgtcctgtc ttggcaaggc gtccgcgtcg
     2521 ccggctcgtt gccgggcaac ggcgtcgact tcggattacg tgccgtccgt ctcgatggtt
     2581 aggaggaagt aatggtgaag tcccgaggat tgatcatcgc gatcgcgttc gtgctcgcag
     2641 gcatagccac gatggtcgtg tacctgtacc tgctcggcgt ccaacgccag agcgaaggtg
     2701 gtgagatggt ctccgtcgtt gtgtcggaac gggacgttcc ggccgggacg agtctggacg
     2761 atctggtcga agagggagcg ctcacgaccc gcgcactccc tcgcagcgct ctcgttcgtg
     2821 gagccgtaac caatctgcag tccctcgagg gacgagagac gagcgccgcg atcgtagccg
     2881 gtgagcaact cacgaccgcc cggttgcgcg gcagcacgac gcagcttccc ggcgggagcc
     2941 tcggcatccc gaacgggtac gcggcgctga cgttgccgct agaagcaccg cgggtcgccg
     3001 ggggggtgat ccaacgctcg gaccacatca cgatctatgg aacgttccag aacgtcgcct
     3061 cacagcaggg aagttccccc gcgtcgacgg tgactttggt gcccgacgtc gaggtcctgc
     3121 gcatcgcaag ctccgctgaa gaaggcggag cgaacgacac ccagtggatc acgctggctc
     3181 tgaagcctcg ggacgcccag aaggtcgtgt tcgctcagga gcaagggtcg gtgtggatgt
     3241 cgctcctgcc tccgaaccaa cgtggaaccg catcccgtcc cgtcaacgcg atccagctgg
     3301 ttcgatgaac cagccggtcg gacctcggat cctcgccatc ggcgcgccgc cgacgttccg
     3361 ccaggacgtg gcgcggtgca tggatgcttc gccagagacg gtcgactggc tgccgagcgt
     3421 cacggccgcc gaagagttcg tggtcgttcg tcacatggaa cccgacgtca tggtgctctc
     3481 gcccgagatc aaggacgccg acgctctcgg cgtcgcggac ttcatggcga agcactcgcc
     3541 cgccaccgca gtgatcctgg ttcgctacac gtcgccgaac ggactcctcc cgatggcgat
     3601 gcgagccggc gtgcgtgacg tcgtagatct gtcccgcgga gaagaggatc ttgccgacgc
     3661 gctgtcacgg gcatccgatt gggctcgagg cgtacgcagc cacacggccg agccggagca
     3721 cgccggcgaa cgcggcaaga tcatctcgat cttctcgtcc aagggagggg tcggcaagac
     3781 cttcctgtcg tgcaaccttg ctgcggcgct ggccgacaaa ggtgaaggcg acaccgcatt
     3841 gttggacctt gacctcaagg tcggtgacgt tttctcctac ttcggtcaag agccaacgcg
     3901 tccgatccag gatctcctct cgctcgccga gcgtaaggat ccggaggcac tcgtcgcgac
     3961 cggcaagcaa ctcggagaca acctgtgggc ctatggcgtg accgccgacc cggcatgtga
     4021 atcaatgcct ggagagacca caggaaagat cgttcgcaac ctccgcgcca acttcgccta
     4081 cacggtcata gatgcatcga acgattactc ggaccaaacc gtcgcggtgt tcgacctatc
     4141 cgacgagatc tggttggtca cgggcctcga cgtagttggg atgcgacacc tcttgatcgg
     4201 gatggacacc ctcacttcgc tcggcatccc acacgaccgc ttccgcgtcg tcctgaaccg
     4261 ttccgactcg aaggtggggg tctctctgac cgatgtggag cgagtgcttc gggttcaggt
     4321 cgacgccaag atcccttcga gcgagctcgt cccgcaatca ctcaacaaag gcaagcccgt
     4381 gtatctcggc gagcccggct ccgaagttgc gagagccatc acggagatcg cagaaaccat
     4441 catcggcact ccacagagcg agaccgcgcc cgcggctcaa caacgacgcg ggctcaagct
     4501 cttgctgggg ctgggatagg aggtaatcgg tgtcgctagt agaacgcttg gcccgttcgc
     4561 agaacggcga tcgtctcggg caggttcgcg atcgagtcca aacccgactg gtcgagagac
     4621 tcgggccgcg cctgaacgac gaatccatct ccgacaagga gctggagtcg atggtccacg
     4681 accggctccg ggatctgctg aacgaagaag agatcccgtt gaccacgacc gagaaggctc
     4741 agatcacgaa agagatccag gacagtgtca tgggtctcgg ccccctcgag gagttgctgg
     4801 ccgatacgga cgtcaccgag atcatggtca acggatgcga gaccgtgttc gtcgagcgcg
     4861 ccggcaagat ccatcgcacg aacgcacgct tcgttgacga gaaccaattg cgccgcacga
     4921 tcgacaagat cgtgacgaag gtcggacgcc ggatcgacga gtcctcgcct tacgtggacg
     4981 cccgtctgcc ggacggctct cgtgtgaacg cgatcatccc gcctctcgcg atcgacggcc
     5041 ccgcgctaac catccgcagg ttctcggccg atccctacgg cacggaggaa ctggtgtcgt
     5101 tcgggacctt cagcggtccc atggccgagt tcctcgaagc gtgcgtgcgc ggccggatga
     5161 acctgctcgt ctcgggtgga accggagccg gcaagacgac gaccctgaac gtgctttcct
     5221 cctacatccc gcacgacgag cggatcctca cgatcgagga tgcggccgag ctgaagctgc
     5281 aacagccgca cgtggtccgg ctcgagtccc ggcccccaaa catcgagggt gtgggtcagg
     5341 tagccatccg cgatctcgtc cgcaacgccc tccgtatgcg ccccgaccga atcatcgtcg
     5401 gggaagtcag ggggggtgag gcgatcgaca tgttgcaagc gatgaacacc ggccacgacg
     5461 gatcgatctc aacgatccac tcgaactcgc ctcgcgacgt cctttcacgt ttggagacga
     5521 tgacgatgat ggcggggatg gagctcaccg tccgagcgat ccgcgagcag atcgcatcgg
     5581 cgatcgacgt gatcgtccat caggagcgca tgaaagacgg aacccgtcgc atcactcacg
     5641 tctccgaggt cgtggggatg gaaggcgaca tcatcacgtt gcaggacatc ttcttgttca
     5701 acttcaaggc cggggtcgat gcgcaaggtc gcgttctcgg ctcactcgag gcaaccggct
     5761 tacgtccagg cttcctcgag aaactcagcg accgcgg
//
LOCUS       DATMED010000456         4655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5359717, whole genome shotgun sequence.
ACCESSION   DATMED010000456 DATMED010000000
VERSION     DATMED010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5359717"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VNP73_11940"
     CDS             <1..380
                     /locus_tag="VNP73_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL66677.1"
                     /translation="ALKAGASGYLLKTADLEEVVSTVRSVAAGGGKLDPTLALKVLSE
                     FQNYQQAEISEGYQPLTPREREILQLMSEGLPNKTIASRLSISERTVTTHVANIYSKL
                     HVNNRVSAIQEAMRRRILNINQY"
     gene            383..1768
                     /gene="selA"
                     /locus_tag="VNP73_11945"
     CDS             383..1768
                     /gene="selA"
                     /locus_tag="VNP73_11945"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HWL66678.1"
                     /translation="MAPSGEADHGSRFRELPSITTLVEELTGAAPEPVLIAVARRAVD
                     LARARIKRGETVAAPDVEREARSLLAEREAGLLQPVINATGVLIHTNLGRVPLGEKQI
                     EAIRRIATSYSNLEFDLTTGSRGTRYAHARSLLTTLTGAEAGVVVNNNAAAVLIVLAA
                     LCAGREVVVSRGELIEIGGEFRLPDVMAASGARLVEVGTTNRTHIRDYETAISPDTAA
                     LMKVHPSNYKVVGFTAEVNARDLARLARGRGVPFIHDLGSGLLEGADAGIATGEPSVQ
                     AALEDGADLVTMSGDKLLGGPQAGIILGRAELVERVIKHPLMRALRVDKLTLAALEAT
                     LAAYLDGSARDHPLWKLALASADELESRAHRLTDSIASRTGDSFKIEAVPTGSVTGGG
                     SLPGTELPSWGLRMLPAQRSADSLERSLRSGSPPIVGRIIDDYVVLDLRCVVPADDEK
                     LADLVVLALQE"
     gene            1825..4101
                     /locus_tag="VNP73_11950"
     CDS             1825..4101
                     /locus_tag="VNP73_11950"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M6"
                     /protein_id="HWL66679.1"
                     /translation="MRRRALSVLCTFGLLAVAVPIARVQAEAARVPQVKVRHVGPDYN
                     GGRMLPIRGGFDATGAIAAAAGPKVGTKREWLGLDDIAGRLYPKDYTLRGKGEHLEAW
                     VANDIDETSTGLNFPGTDCRNDERVLITNKQVKYLINQFDKVMFPRESKIFSTPPKRD
                     GSDALLYDPSVTSPDPAKPRGYYKGRGDRVVVLIDNVRDDNFYDTDNSENLPYIAGFF
                     FSGYNELFDRNVMTIDAYDWIHRTHKNPLHEPVPGDNCASKPARPFLYEGTFAHEYQH
                     LLEYYRDPDEAPWTNEGLSDLAQTVTGYVDPRTPITESGYDGHIQAFLGWLEVQTDAN
                     PNPRAGGPENSLNLWEEPQQPGEVLSDYGGAYTFFEMLKHRYGGRFIRKVHRHPLNGL
                     ESLDTLLQDTKAKKSARQVLHEWSAMVALDGVLDDGATLTGAAPGVFKTKTLDSSINW
                     DNDDTFSYPGAPPNGSDFVRLRDGDGAYVNSSAIESISFDGSDSLPAVPIEWEVDQDP
                     PDHNNAALYSGSGASFDRAIVEEVNVPAQDPQLTFETRYEVEEGWDYGFVQVSDDGGE
                     SWTSLENDNTIFDSDPGAIDLVKDNLPGFTGNSGCEPGSQLGGDCTATWVTETFDLSD
                     YAGEDILLSFRNITDPGVNLPGWWIDEIRVGNKNVSNGTSINEWQTPTEINPHAVEGF
                     TLQLIAYNDAHTLAGYTAVPLGSGFTAELDEADLAFLPDSAETVAALVMYDESTEQIL
                     QYAPYSLQVNGVTQPGGG"
     gene            4132..>4655
                     /locus_tag="VNP73_11955"
     CDS             4132..>4655
                     /locus_tag="VNP73_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWL66680.1"
                     /translation="MNLHRALRIATGEIHHWQADTYVVSYPKSGRTWLRVLIAKAIAV
                     EARHLDDIFDLNKAPASVKRAAPSLRFSHDWSGRNYRWPGPRYDRYSRKKVVFLARDP
                     RDVVVSHFFQLTKRAVAYSVDCPQTMSEFIRNSSWGVERVVHFMNDWYEHKSEPTSFL
                     MVRYEDLQRDPHKEL"
BASE COUNT          962 a         1491 c         1402 g          800 t
ORIGIN      
        1 acgcactcaa agcgggcgcg tccggctacc tactcaagac cgcagatctc gaagaagtgg
       61 tttccaccgt gagatccgtc gcggccggcg gcggcaagct cgatccgaca cttgcgctca
      121 aggttctgtc cgaattccag aactaccagc aggccgagat ctccgagggc taccagccac
      181 tcacaccgcg cgaacgcgag atcttgcaat tgatgagcga gggattgccc aacaagacga
      241 tcgcaagtcg tctctcgatc tcggagcgaa ccgtgacgac gcacgtcgcg aacatctact
      301 cgaaactgca cgtgaacaac cgcgtgtcgg cgatccaaga ggcgatgcgg cgccggatac
      361 taaacatcaa ccagtactga cgatggcccc ctcgggcgaa gcagatcacg gatcgagatt
      421 ccgagagctc ccgtccatca ctacattggt cgaggagttg accggggctg ctcccgaacc
      481 ggtactgatc gcggttgcgc gccgagccgt cgacctggcc agagcgcgca taaaacgagg
      541 tgagaccgtc gcggcccccg atgtcgaacg ggaagcacga tccctcctcg ccgaacgaga
      601 ggcgggcctc ctacaacccg tcatcaacgc gaccggcgtc ctgatacaca cgaacttggg
      661 ccgcgttcct ctcggcgaga aacagatcga ggcgatccga aggatcgcga cctcctactc
      721 gaatctcgaa ttcgacctca cgaccgggtc ccgcgggacc cgctacgcgc acgcacgttc
      781 tttgctgacg actttgaccg gagccgaagc gggggtggta gtgaacaaca acgcggccgc
      841 cgtactgatc gtgctggcgg ctctgtgcgc gggacgcgag gtcgtcgtta gccggggaga
      901 attgatcgag atcggtgggg aattccgtct gcccgacgtg atggccgcgt ccggagcccg
      961 gctcgttgag gtcggaacga cgaaccgcac gcacatacgg gactacgaaa ccgcgatctc
     1021 acccgacact gccgctctta tgaaagtgca tccctccaac tacaaggtcg tgggcttcac
     1081 tgccgaagtg aacgcgcggg atctggcccg actggctcga gggcgcgggg tcccgttcat
     1141 ccacgacctg ggctcgggtc tgctcgaggg cgccgacgcc gggatcgcga ccggcgagcc
     1201 ttcggtccaa gccgctctgg aagacggtgc cgacctcgtg acgatgtccg gtgacaagct
     1261 cctggggggg ccgcaggcgg ggatcatcct gggccgagcc gagctcgtcg agcgagtgat
     1321 caaacatccg ctgatgcgag ccttgcgagt ggacaagctg acgctggccg ccctcgaggc
     1381 gacgctggcc gcgtatctgg atggctccgc acgagaccac ccgttgtgga agctggccct
     1441 cgcttctgcc gacgagcttg agagcagggc gcaccgcctg accgactcga tcgcctcgcg
     1501 cacgggcgat tccttcaaga tcgaagccgt cccgaccggc tcggtgaccg gtggaggctc
     1561 ccttcccgga accgagttgc cctcgtgggg gctccgcatg ttgccggccc aacgctcggc
     1621 cgattcgctg gaacgcagcc tccgttccgg ctcccccccg atcgtgggcc ggatcatcga
     1681 cgactacgtc gtcctagacc tcagatgtgt ggtcccggcc gacgacgaaa aactcgccga
     1741 cttagtcgtc ttagcacttc aggagtagta aaagtaaacc tgccggttac aagcaacagt
     1801 cgatcgaacc caagggggtc atcagtgcgt cgtcgtgccc tgagcgttct ttgtaccttc
     1861 ggtctcctgg cggttgccgt acctatcgcc cgggtgcagg ccgaagcggc ccgggtgcct
     1921 caagtgaagg tccgtcatgt gggacccgac tacaacgggg gccggatgct tccgatccgc
     1981 ggaggcttcg atgcaaccgg agcgatcgcc gctgcggcag ggccgaaggt cgggacgaag
     2041 cgagagtggc tgggcttgga cgacatcgcc ggccggctct atcccaagga ctacacgctg
     2101 cgaggcaagg gtgagcacct cgaggcatgg gtcgcgaacg atatcgacga gacctcaacc
     2161 ggtctcaact tccccggcac cgactgtcgc aacgacgaac gcgtcttgat cacgaacaag
     2221 caggtcaagt acctgatcaa ccagttcgac aaggtcatgt tcccccgaga gagcaagatc
     2281 ttctccaccc cgccgaagcg cgacggatct gatgcgctcc tgtacgaccc gtcggtaacg
     2341 tctcccgacc cggcaaagcc acggggctac tacaagggcc gcggggaccg cgtcgtcgtt
     2401 ctcatcgaca acgtgcgaga cgacaacttc tatgacacgg acaactcgga gaacctcccc
     2461 tacatcgccg ggttcttctt ctcgggctac aacgagctgt tcgatcgcaa cgtcatgacg
     2521 atcgatgcct acgactggat ccaccgcacc cataagaacc ctctgcacga gcccgtgccc
     2581 ggagacaact gtgcgtcgaa gccggcgcgg cccttcctct acgaaggaac gttcgcgcac
     2641 gagtatcagc atctgctcga gtactaccgg gacccggacg aagcgccgtg gacgaacgag
     2701 ggtctgtccg acctcgcgca gacggtcacc ggctacgtgg accctcgcac gccgatcact
     2761 gagagcggct acgacgggca catccaggct ttcctgggct ggctcgaggt ccagaccgat
     2821 gccaacccga acccgcgggc gggggggccg gagaactcgc tgaacctatg ggaagaaccg
     2881 cagcagccgg gtgaggtcct gtccgactac ggcggcgcgt acacgttctt cgagatgctc
     2941 aagcaccggt acggcggccg attcatccgg aaggtgcacc gccatccgct gaacgggctc
     3001 gagagtctcg acacccttct tcaggacacg aaggcgaaga agagcgcgcg ccaggtcttg
     3061 cacgaatggt cggcgatggt ggctctggac ggcgtcctcg acgacggggc gaccctgacc
     3121 ggtgccgcgc ccggcgtctt caagaccaag accctggatt cgtcgatcaa ctgggacaac
     3181 gacgacacat tttcgtaccc cggcgcacct ccgaatggat cggacttcgt ccgccttcgg
     3241 gatggagacg gggcttacgt caacagctct gcgatcgaat ccatctcgtt cgacggttcc
     3301 gacagcctgc ctgcggtccc catcgagtgg gaggtcgatc aggacccacc cgaccacaac
     3361 aacgcggccc tctactccgg ttccggcgcg agtttcgacc gggccatcgt cgaagaggtg
     3421 aacgttcccg cacaagaccc gcaactcacg ttcgagaccc gctacgaggt ggaagagggc
     3481 tgggactatg ggttcgtgca ggtctccgat gacggaggcg aatcctggac gagcctggag
     3541 aacgacaaca ccatcttcga ttccgatccc ggcgcgatcg atctggtgaa ggacaacctg
     3601 cccggcttta ccggcaattc cggctgcgag cccggctcgc agctcggcgg cgactgcacc
     3661 gcaacgtggg tgaccgagac cttcgacctg tccgactacg ccggcgaaga catcctgctc
     3721 tcgttccgta acatcaccga tccgggtgtc aacctccccg gctggtggat cgacgagatc
     3781 cgcgtgggca acaagaacgt ctccaatggg acgtccatca atgaatggca gaccccgacg
     3841 gagatcaacc cgcacgccgt cgagggattc acgttgcagc tgatcgccta caacgacgcg
     3901 cacacgctcg ccggttacac cgcggtcccc ttggggtccg gtttcacggc cgaactcgac
     3961 gaagcggacc tcgccttctt gccagactcg gcagaaaccg ttgcggcact cgtgatgtac
     4021 gacgaatcga ccgaacagat cttgcaatac gcgccgtata gtttgcaggt caacggagtt
     4081 acacaacccg gcggcggcta atctcgaaat gcttgataag acgagcgcgg attgaatctc
     4141 catcgggcgc tccggatcgc caccggagag atccaccatt ggcaggccga cacttatgtc
     4201 gtttcgtacc cgaagtccgg ccgaacctgg ctgagagtgc tgatcgccaa agcgatcgca
     4261 gttgaagccc gacacctcga cgacatcttc gatctgaaca aggccccggc atcggtgaag
     4321 agggccgctc cttctctcag attttcgcat gactggtccg gccgaaacta tcgatggccg
     4381 gggccaagat acgaccgata ctcgagaaag aaagtcgtct tccttgcgcg agatccacga
     4441 gatgtcgtgg tctcgcattt ctttcaacta acgaagcgtg cggtggcgta ctcggtcgat
     4501 tgtccgcaaa ccatgagcga attcattcgt aatagcagtt ggggagtgga gcgcgtcgtc
     4561 cacttcatga acgactggta cgaacacaag tcggaaccaa catcatttct catggtgcgg
     4621 tatgaggact tgcaaagaga tcctcacaaa gagct
//
LOCUS       DATMED010000457         6404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4213155, whole genome shotgun sequence.
ACCESSION   DATMED010000457 DATMED010000000
VERSION     DATMED010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4213155"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VNP73_11960"
     CDS             complement(<1..780)
                     /locus_tag="VNP73_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66681.1"
                     /translation="MRALIVGKGHERGSLAAARSLTAAGWEVGSGAPSGRGLVSLSRA
                     VARRHEIPLISRDGDAFIAAVREVVRETSYDVIFSSSDAELLALSEARADLGAVFPYP
                     PHGSLLSVLDKKQLDPRAREVGFNVPEVIEDPDGQLDRLGYPVVVKDRLHSARLGIGI
                     ERVEATAARDPSEAEEALLEVRAHGGEPVVQRFVPGALMAYVCLIDSEGTVVAECQQL
                     AEAIWPHPAGVSTRSQTIPVDSAVAESARALLSGLRWQGLAE"
     gene            complement(768..1931)
                     /locus_tag="VNP73_11965"
     CDS             complement(768..1931)
                     /locus_tag="VNP73_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66682.1"
                     /translation="MKARTYIHARYPWRAIVQVGGPAASYYAELISQGLKYGLPAKRF
                     GRLGAILAIRGTPRSVLALGGRTGTRGAAPEIDRVVDELQARWGDVRARLTSVPDDPS
                     ELFALGLHRSAGLTVFFVDGSGAPVLVAKVPREGNQGIAVEESALKRVEGIGVAPLLV
                     GSFADVVIQEGVPGEPSALVPLSPDDVCEVPWSAEHRAIADALTRLASHSVTLHHADE
                     LDPWLDEVDRNDDLSRGIRSSVSSAAAAIRGAGISVLRHGDTSVQNCLTRNGSLAALV
                     DWERAQHRGVPGFDTLNFALAWLDHSLGLRRWSESLSFEAFDRAWDSSPLMRSARAAA
                     RAAATAAGLGDSVLDPMEVTFFARRLGRRLADPDAYATGSRVAARMLERVCAL"
     gene            1979..3403
                     /locus_tag="VNP73_11970"
     CDS             1979..3403
                     /locus_tag="VNP73_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWL66683.1"
                     /translation="MALHATRPALDLQRSFLLLLVAASCAAVGAGLAFVGVRLGALGP
                     LFVIGLPAAGILAALSFDRPLIGIAAIVISFPFGTIAIPTGSVTLQAVEVAVVLVTVV
                     VALHRLARGTTMLPWTSHLWWYLALIGWTLIALPSAIDQALALKQIAQLVGGLLLATS
                     LVAACRDMSDVRRLMTLFLAVAAGIAIVALLNGQQLDAGFGGSYVSGRAKGTFDQPNQ
                     LGSFSAVAALVGTGLILGARRWGERMLIIGCLIVVAGAMLLSLSRGAWIGAAGAALFL
                     ALALREYRRLILAVSIPLLVLGAMAGAIAPSNPQVVVVTQRVESLTVLSPYDNRPAIW
                     AEAFREIRADPWTGQGPGSFPVASARASSEATTSSAQHAHNMWLTWAAESGLPGALIL
                     TGFIAALAVAGAQAARAATDARDRALVIGLCAGLIVIAGQGTVDYTLRNAVVFFSVWT
                     VVGGLLASWRILTSRAPTSSVTAP"
     gene            complement(3363..4493)
                     /locus_tag="VNP73_11975"
     CDS             complement(3363..4493)
                     /locus_tag="VNP73_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HWL66684.1"
                     /translation="MGSRRTVIAVVVAAALCAPSSGSVAAPTVTSGATRAVGARNHPQ
                     MGVQFHAMWSDYNDTQRRKVLNKMAAAGVKWVRIDVGWSSLQEQGRGVIGDWYADLLD
                     EVVDETRKRGMHVLATLWRTPAWANNGAGVIAPPTDASEYGRIAEWAARRYRGRIEAW
                     EVWNEPNLSDFFRGSAADYAKLLKASYRRFKQGDPNAKVLMGGPSANDTDWLERVYKA
                     GAAGHFDIMATHPYQGMADYPPEHPDDGNVWWLTHTPAVRRLMKRFGDGNKKIWFTEF
                     GWSSHRNWKGVENWDRGVSAKVQGKYLVRTLKFVAREYPYVKKVFWYNERNRKTGDKH
                     LDNYGLLKRDLTPKPAYRAMKRFLTAEPAATARLLMMSERAT"
     gene            4623..5903
                     /locus_tag="VNP73_11980"
     CDS             4623..5903
                     /locus_tag="VNP73_11980"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HWL66685.1"
                     /translation="MIQERALPDLLDAVRRRWPIVLVMAISVAAGAAYYVESLPNQYE
                     ATALVALSPNTRVEASADTVRVVGPKYAEYLTAPSTIEEVAALVDQDPAILTDAIDAS
                     LAVDTGNLTVTAELSSPEEAAEVANAFADAADDLAREDRLLSGEVLAEAIAPEEPSGP
                     PRRLLEGAALLAGLLLGTAFAVVIERGRPRLNNIQDIARVTGHPVIGRIPRSKLLRRS
                     PRDWQTDPIVGSAFRTLRANMDAQWRDRTIDSIVITSPGPGDGKTTVATVFAASLARL
                     GASVLLIDADLRRPNVKQLIQPDEPGDLAAVLRKDSEFETEIHPEWVDGLWVMTTSAD
                     PDAGDLLARRFSEVLQKAREKFDIVIVDSPPVVTTDDARTLATIASGVLIVVSQRSDV
                     KEVTEAVLALEALKAPVLGIVANRVRESAKAYYG"
     gene            complement(5894..>6404)
                     /locus_tag="VNP73_11985"
     CDS             complement(5894..>6404)
                     /locus_tag="VNP73_11985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWL66686.1"
                     /translation="CVGPLRHQKGQDLLLTAWPLVRREIPDATLVLVGAGSERGRLES
                     LAGPGVVFAGHREDVVTWLAAAAVVTIPSRWDGLSLSLLEAMSCARSVVATDTPGAGR
                     VLNGGGEVVPIGDTGALAGALTTRLRDPSLAAAEGDRGRARVEQDFDWRRVIEQMNAA
                     YGRVISGQP"
BASE COUNT         1150 a         2179 c         1941 g         1134 t
ORIGIN      
        1 ctcggccaag ccctgccacc gaagaccgct caatagtgcc cgcgcgcttt ccgccactgc
       61 actgtccact gggatcgtct gcgaacgcgt gcttaccccg gccgggtgag gccagatcgc
      121 ttccgcgagc tgctgacatt ccgcgacgac cgtgccctcc gagtcgatca ggcacacata
      181 agccatcaac gccccgggca cgaatcgctg gacgaccggc tcgccaccgt gcgcacgcac
      241 ttccaaaaga gcttcttcgg cttcggacgg gtcccgagca gcggtcgctt ccacgcgctc
      301 gatacctatg ccgaggcgag cgctgtgaag acgatccttg accacgaccg gatacccgag
      361 ccggtcgagt tgcccgtccg gatcctcgat gacctccgga acattgaagc cgacctcccg
      421 ggcacgagga tccaactgtt tcttgtcgag aacgctcagc aatgaaccgt gcgggggata
      481 cgggaacact gcccccaggt ccgctcgcgc ttcggagagc gccagcagtt ccgcgtcgct
      541 ggaagagaag atgacatcgt aagaagtctc tcggacgact tctcgaaccg cggctatgaa
      601 cgcatcccca tctcgcgaga tcagagggat ctcgtgacgc cgcgctacgg cgcgtgacaa
      661 ggacaccaag cctcgacccg acggcgcacc ggatcccact tcccagccgg cggcggtgag
      721 agacctcgcc gcggccaacg acccgcgctc gtgacccttc ccgactatca gagcgcgcat
      781 acgcgctcca gcatcctcgc cgcgactcga gatccggtcg cgtacgcatc agggtctgca
      841 agcctgcgcc cgagacgtcg cgcgaagaat gtgacctcca tcggatcgag aaccgagtcc
      901 cccaagccgg cggccgtggc cgccgcacgt gcagccgctc ttgcactgcg catcaagggc
      961 gatgaatccc acgcgcggtc gaaggcctcg aacgagagag actccgacca acgcctcagc
     1021 ccgagcgaat ggtccagcca cgccagcgca aagttcagtg tgtcgaagcc gggtacgccc
     1081 cgatgctgag ctcgctccca atccacgagc gcggccaatg acccgttgcg cgtcagacag
     1141 ttctgcaccg acgtatcgcc gtgtcgcagc accgagatcc ccgcgcccct gatagcagcg
     1201 gcggcggaag agaccgacga tctgatccct cgcgacaggt cgtcattacg gtccacctca
     1261 tcaagccatg ggtccagctc gtcggcgtga tggagtgtta cggagtgaga cgctagtcgg
     1321 gtcaaggcat ccgcgatcgc tcgatgttcc gccgaccatg gaacctcgca cacgtcatct
     1381 ggtgacagag gcactaaggc ggaaggctct cccggaacgc cttcctggat cacaacgtcg
     1441 gcgaacgagc ccaccaacaa aggcgcaaca ccgatcccct caacacgttt cagcgcgctc
     1501 tcttcaacgg cgatgccctg attcccttcc cgtggaacct tcgcgaccaa aactggggca
     1561 cccgacccgt ccacgaagaa caccgtcaga cctgcggaac ggtgcaggcc caacgcgaac
     1621 agctcgcttg ggtcgtccgg aacggacgta agacgcgcgc gcacatcacc ccaccgcgcc
     1681 tggagttcgt cgacgacgcg gtctatctct ggtgcagccc ctctcgtacc cgtgcggccg
     1741 ccgagtgcga gcacgctccg cggagtccct ctgatcgcca ggatggcccc caaacggccg
     1801 aaccgcttgg ccggcaagcc gtacttgagt ccttgggaga tcagctccgc gtaatacgaa
     1861 gcggccgggc ctcccacctg aacgatggcc cgccacggat atctggcatg gatgtacgtc
     1921 cgagccttca ccgaagcatg ctaccgacga gtgacggcgc atacgcgaaa ctgtgtctat
     1981 ggcgctgcac gcgactcgac cggccttgga tctacagagg agtttcctgc ttctgctcgt
     2041 agccgcatcc tgcgccgcgg tcggagcagg actggctttc gtcggagtac gactaggcgc
     2101 cctgggacct ctgttcgtca tcgggctccc agccgcgggg attctggccg ccctttcttt
     2161 cgacagaccg ttgatcggga tagcagcgat cgtgatctcg ttcccatttg gaacgatcgc
     2221 gatcccgacc ggttccgtga cactgcaggc ggtcgaggtt gccgtcgtcc tggtaacggt
     2281 cgtggttgcc ctgcaccgac ttgctcgcgg aacgaccatg ctcccctgga catcgcatct
     2341 gtggtggtat ctcgccctta tcggttggac tttgatcgcg ctgccgtcgg cgatcgacca
     2401 ggccctcgct ctgaagcaga tcgcgcagtt ggttggaggg ctgttgctcg ccacgtcttt
     2461 ggtagctgcc tgccgcgata tgagcgacgt gcgccggctg atgacattgt tcttggcggt
     2521 ggcggccggc attgcgatcg tggctttgct caacggtcag cagttggacg ccggcttcgg
     2581 gggttcgtac gtaagtggac gcgccaaagg taccttcgac caacccaatc aactcggttc
     2641 cttctcggcc gttgccgccc tggtagggac gggcttgatc ctgggcgccc gccgctgggg
     2701 cgaacgcatg ctgatcatcg gctgtttgat cgtggtggca ggagcgatgt tgctttctct
     2761 ttcccgagga gcgtggatcg gcgcggcggg cgcagctctt ttcctggccc tggcattgcg
     2821 cgaataccgc aggctgatcc tcgccgtctc cattcccctg ttggtgctgg gggcgatggc
     2881 cggagccatc gcgccgtcca atccccaggt tgtcgtcgtg acgcaaaggg tcgagtccct
     2941 cacggtctta agcccctacg acaatcgacc ggccatctgg gcggaagcgt tccgagagat
     3001 ccgtgccgac ccgtggaccg gtcagggacc ggggagtttc ccggttgcca gcgcacgagc
     3061 ttcctcggag gcgacaacct cgtccgccca acacgctcac aatatgtggc tcacctgggc
     3121 ggcggagtcg gggctgccgg gggcgctgat attgaccggg ttcatcgcgg ccctcgccgt
     3181 cgccggcgcg caagccgccc gcgctgcgac cgacgccaga gacagagcgt tagtgatcgg
     3241 gctttgtgca ggcctgatcg tgatcgcggg gcaagggacc gtcgactaca cgctccgtaa
     3301 cgccgtcgtg ttcttctcag tctggacggt ggttggagga ctactcgcgt cgtggaggat
     3361 ccttacgtcg cgcgctccga catcatcagt aaccgcgccg tagcggcggg ctcggccgtt
     3421 aggaagcgtt tcatcgcccg atacgcgggc ttcggagtca gatccctctt cagcaaaccg
     3481 tagttgtcga ggtgcttgtc gcctgttttc ctgtttcgct cgttgtacca gaagaccttt
     3541 ttcacgtacg gatactcgcg ggcaacaaac ttgagcgtgc gcacgagata cttgccctgc
     3601 accttcgcgg agacgccgcg gtcccagttc tcgacacctt tccagtttcg gtgcgatgac
     3661 catccgaact cggtgaacca gatcttcttg ttcccatcac cgaaccgctt catcaagcga
     3721 cgcaccgcgg gggtgtgagt caaccaccac acgttgccgt cgtcaggatg ctccggcggg
     3781 tagtcggcca tcccctggta ggggtgcgtc gccatgatgt cgaagtgacc ggcggctcct
     3841 gccttgtaaa cgcgctccaa ccagtccgta tcgttggctg agggcccacc cataagcacc
     3901 ttcgcgttcg ggtctccttg cttgaagcgc cgataggagg ccttcagaag tttcgcgtaa
     3961 tccgccgcgc tccctctgaa gaaatccgag agattcggtt cgttccaaac ctcccacgcc
     4021 tcgatgcgac cgcggtaacg acgggcggcc cattcggcga tccgcccgta ctcggacgca
     4081 tccgtcggag gagcgatgac ccccgctccg ttgttcgccc acgcgggcgt acgccacaac
     4141 gtggcgagca cgtgcatccc gcgcttgcgc gtctcgtcga cgacctcgtc gagaagatcg
     4201 gcgtaccagt cgccgatgac cccgcggccc tgctcctgga gtgaagacca ccccacgtcg
     4261 atccgcaccc acttgacacc ggcggcagcc atcttgttca agaccttcct gcgctgcgtg
     4321 tcgttgtagt cgctccacat cgcgtggaac tggacaccca tctgcggatg gttgcgggca
     4381 ccgaccgcgc gcgtcgcgcc tgaggtgacc gtgggggccg cgaccgaccc gctcgacggc
     4441 gcgcagagag cggcggcgac cactactgcg atcactgtgc gtctcgagcc caccccaact
     4501 cccccttgcc cggtattact aacccctcgg cagccgagaa gccaccttta ggtagctttc
     4561 ccatgttttg tgaggaaaat cttcgactag ggtccacatc cgggcaatac gcaaggagag
     4621 cagtgatcca agaacgagct ttaccggatc tactcgacgc cgtccgtagg cggtggccga
     4681 tagtcctggt gatggcgata tcagtggctg cgggggcggc gtactacgtc gaatccctcc
     4741 ccaaccagta tgaggctacg gccctagtgg ccctgagccc caatacacgg gtggaggcgt
     4801 ccgcggacac ggtcagggtc gtggggccga aatatgcgga gtacctgacc gctccctcga
     4861 cgatcgagga ggtcgccgcc ctggtggatc aggaccccgc catcctgacc gacgctatcg
     4921 atgcctcgct ggccgtcgac accggaaacc tcaccgttac ggcggagctg tcctcacccg
     4981 aagaagcggc cgaggtagcg aacgcattcg ccgacgccgc cgacgacctg gcgcgagagg
     5041 atcgcctgct gtccggggag gtgctggcgg aagcgatcgc accggaggag ccttccggcc
     5101 cccctcggag attgctcgaa ggggccgcgt tactggccgg cctgctactc ggaaccgcgt
     5161 tcgcagtcgt catcgagcga ggccgaccga gactcaacaa catccaggac atcgcacggg
     5221 tcaccggcca ccccgtcatc ggacggatcc cgcggtcgaa actcctccgc cgaagccctc
     5281 gtgactggca gacggatccg atcgtcggct cggcgttccg cacattgcgt gccaacatgg
     5341 acgcgcagtg gcgagatcgg acgatcgact cgatcgttat cacctccccc gggcccggtg
     5401 acggcaagac cacggtagcc accgtcttcg ccgcctccct ggcgcgcctc ggagcctcgg
     5461 tgctgttgat cgacgccgac ctgcgacggc cgaacgtcaa gcagttgatc cagcccgacg
     5521 agcctgggga cctagccgcc gttctgcgga aagactccga attcgagacc gagatccacc
     5581 ccgaatgggt cgacgggctg tgggtcatga ccacttccgc cgatccggat gccggcgacc
     5641 tcctggcgcg acgtttctcg gaagtccttc agaaggcgag agagaagttc gacatcgtca
     5701 tcgtggacag ccctcccgtg gtcacgaccg acgacgcacg gacgttggcg accatcgcga
     5761 gcggagttct catcgtcgtt tcgcaacgct cggacgtcaa ggaagtaaca gaagccgtcc
     5821 tcgccctcga agcgctgaag gcacccgtgt tggggatcgt cgccaaccgg gtcagagaat
     5881 cggctaaggc ctactacggc tgacccgaga taactcttcc gtaggcagca ttcatctgct
     5941 cgatcacgcg ccgccagtcg aagtcctgct cgacgcgcgc gcgaccacgg tctccttctg
     6001 cggctgcaag ggaagggtct ctgagacggg tcgttagagc acccgccagg gcgccggtgt
     6061 cgccgatcgg aaccacctct cccccgccgt tcaatacgcg cccggccccc ggggtgtccg
     6121 tagccaccac actgcgcgcg cacgacatcg cttctagcaa cgacagcgac agaccatccc
     6181 agcgcgacgg gatcgtcacg acggcggcgg cggccagcca cgtcacgacg tcctcccggt
     6241 gtccggcgaa cacgaccccg gggccggcga gagactcgag ccgaccacgt tccgaaccgg
     6301 cccccacgag cacgagagtt gcatccggta tctcacgccg aacgagaggc cacgcggtga
     6361 ggagcagatc ctggcctttc tgatggcgca gcggacccac acag
//
LOCUS       DATMED010000458         3626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_5361748, whole genome shotgun sequence.
ACCESSION   DATMED010000458 DATMED010000000
VERSION     DATMED010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5361748"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VNP73_11990"
     CDS             <1..958
                     /locus_tag="VNP73_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWL66687.1"
                     /translation="RRIDIAKILAPVALATLLVACGGEPTTSTGGEGSGLSGEVAIDG
                     SSTVFPIAQAVAEDFQIENPEVKVSVGFAGTGGGFEAFCAGDTQISNASRAIEPEEAD
                     CLKKAGIGYTEFQIGVDGLAIVTHPDTEWVDCITFEQLQAIYAPGSKVETWKDVDPSW
                     PDEPLKIFGPDPDSGTYDYFAEEVADPDAEEPATRDDMTQSADDNVLVQGVEGEQASI
                     GYFGFAYYQENVQGLKVIEVDDGESGCVEPSAETVKSNTYPLSRPLFFYVANDAIEQD
                     VVAQFVNFWVANSADYATAVGYIEVSQDAELANDEALTQLTG"
     gene            990..1895
                     /gene="pstC"
                     /locus_tag="VNP73_11995"
     CDS             990..1895
                     /gene="pstC"
                     /locus_tag="VNP73_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWL66688.1"
                     /translation="MATETFTLTSHRKRPVERTIKIALFGCAAFSVLITVGIIYTLLV
                     DTITFFGNVSIVDFLTKANWSPQSGSFGVLELVWGTMIITVGAMLVAGPLGVATAVYL
                     SEYAPARVRRVVKPILEILAGVPTVVFGYFALNFITPELMRAIYSDCGIFNWGSAALV
                     LGLFILPTVASLSEDAMRAVPSSLRQAGYGLGSSKLRVSMRVVLPAALSGVVAALILG
                     ISRAVGETMAVTIAAGATPQIAFSPCASVQTMTAYIVQVAGGEASTGSVEYGSIFALG
                     STLFAITLLLNSLSIRFVKRFREVY"
     gene            1895..2776
                     /gene="pstA"
                     /locus_tag="VNP73_12000"
     CDS             1895..2776
                     /gene="pstA"
                     /locus_tag="VNP73_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWL66689.1"
                     /translation="MAVRIETLTRRRSWTTRFGDRLAEASLLAATAVGLIALTVLLWT
                     VLDQGATALNWPFIRNFASRIPERAGIWPPLVGTVYLMLLVTAASFPLGVGAAIYLEE
                     FAPRNRLTSAIETNIANLAAVPSIIYGLLGLALFYRAMNLGRSLLTGALTLTLLVLPV
                     IIIATREALRAVPHSIRLGAMALGATRWEAVYKQVLPIAIPGIMTGTILGLSRAIGET
                     APLLVAGAAAFVAFTPDHLLDAYSALPIQIFDWVRRPQPDFQALAAGAILVLLALLLT
                     MNAIAIVIRNRYYRRNR"
     gene            2773..3609
                     /gene="pstB"
                     /locus_tag="VNP73_12005"
     CDS             2773..3609
                     /gene="pstB"
                     /locus_tag="VNP73_12005"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWL66690.1"
                     /translation="MIVETELVRDDIPIPEEHPAPVFQVRDVAVTYGGQPAVEGITMD
                     VPSDRITALIGPSGCGKSTLIRCFNRMNDLIPGAAIAGRIDYHGLDLYGPNVDPVEVR
                     RRIGMVFQQPNPFPKSIYENVAFGLRVNKMTDDLDARIEKALRDAALWDEVKRKLKTP
                     ATSLSGGQQQRLCIARALATAPDVLLMDEPCSALDPIATTKIEDLMHQLKTNYTILIV
                     THNMQQAARVSDRTAFLTVQGSEEEGIIPRGVLVEFDDTERIFTNPSDSRTEDYITGR
                     FG"
BASE COUNT          705 a         1129 c         1090 g          702 t
ORIGIN      
        1 cagacggatc gatatcgcga aaatcctcgc cccggtagcc cttgcgacct tgctcgtggc
       61 gtgcggcgga gagccgacga cgtcaacggg aggcgaagga tcgggcctct caggcgaggt
      121 tgcgatcgac ggttcgtcga ccgtattccc gatcgctcaa gcggtcgccg aagacttcca
      181 gatcgagaac cccgaagtga aggtctccgt cggattcgcc ggtaccggcg gcggtttcga
      241 ggccttctgc gccggagaca cccagatctc gaacgcttcc cgagcgatcg agcctgagga
      301 agccgactgc ttgaagaaag ccgggatcgg ctacaccgag ttccagatcg gagtggacgg
      361 cctggcgatc gtgacccatc ccgacaccga gtgggtggac tgcatcacgt tcgaacaact
      421 ccaggccatc tacgcgccgg gaagcaaggt cgagacctgg aaggacgtcg atccgtcctg
      481 gcccgacgag ccgctcaaga tcttcggccc ggaccccgat tccggcacct acgactactt
      541 cgctgaagag gttgccgacc cggatgcaga ggagccggcc acccgagacg acatgacgca
      601 gtcggcggac gacaacgtct tggtccaggg tgtcgaaggc gagcaggcct cgatcggcta
      661 cttcgggttc gcctactacc aggagaacgt gcaaggtcta aaggtcatcg aggtcgacga
      721 tggtgagagc ggttgcgtcg agccgtcggc cgagaccgtt aagtccaaca cctacccact
      781 ctccagacct ctcttcttct acgtcgcgaa cgacgcgatc gagcaggacg ttgtggcgca
      841 attcgtgaac ttctgggttg ccaactctgc cgactacgcg accgccgtcg gctacatcga
      901 agtatcccag gacgccgagc ttgccaacga cgaggcccta acgcagctca cgggataggc
      961 ggatcgcgtc aaccggatcg ggggagacgg tggcaaccga gacattcaca cttacatcgc
     1021 ataggaagcg gccggtcgaa cggacgatca agatcgctct gttcggctgt gccgcgttca
     1081 gtgtcctgat aacagtcggg atcatctaca cgctgctcgt ggacaccatc acgttctttg
     1141 gaaacgtcag catcgtcgac ttcttgacga aggcgaactg gtcgccccaa tcaggatcct
     1201 tcggagtcct ggagcttgtg tggggcacga tgatcatcac ggtcggagcg atgctcgttg
     1261 ccggccccct gggagtggcg acggccgttt acctgtccga gtacgcgccg gcccgcgtac
     1321 gccgggtcgt gaaaccgatc ctcgagatcc tcgccggagt accaaccgtc gtattcggct
     1381 acttcgcctt gaatttcatc acccccgagc tgatgcgcgc gatctacagc gactgcggga
     1441 tcttcaattg gggttcggcg gctttggtcc tcggattgtt catcctccca acggtcgctt
     1501 ctctttcgga ggatgcgatg cgtgcggttc catcgtctct ccgccaagcg gggtacggat
     1561 tggggtcatc gaaactccgc gtctcgatgc gcgtagtcct gcccgcagcc ttgtccggcg
     1621 tggtcgcggc tctgatcctc ggaatctctc gcgcggttgg agaaaccatg gctgtaacca
     1681 tcgcagccgg ggcaacgccc cagatcgcgt tcagcccctg tgccagcgtt caaaccatga
     1741 ccgcatacat cgttcaagtg gcaggaggcg aagcctcgac gggatcggtc gagtacggat
     1801 ccatcttcgc actgggcagc acgctcttcg cgatcactct gctcctcaat tctctgagca
     1861 tcagattcgt aaaacgattc cgagaggtgt actgatggcc gtccgcatcg aaacgctcac
     1921 gcgcaggcgc tcctggacaa cgcggttcgg agaccgtctc gcggaggcgt cactcttggc
     1981 tgcaactgcg gtggggttga tcgcgttgac cgttctgctg tggacggttc tggaccaggg
     2041 agcgaccgct ctcaactggc cgttcatcag aaacttcgct tcgcgcatac ccgagcgagc
     2101 cggcatctgg ccgccattgg tcggaacggt ctacctgatg ctcctggtga cggccgcttc
     2161 gttccccttg ggcgtggggg ccgctatcta cctcgaggaa ttcgcaccgc ggaaccgcct
     2221 gacgtcggcg atcgaaacga acatcgccaa ccttgccgcg gtcccgtcga tcatctacgg
     2281 gctgctcggg ctcgcgctct tctaccgggc catgaacctc ggacgcagcc tgctcaccgg
     2341 tgcgctgaca ctcactctgt tggtgctccc cgtgatcatc atcgcgacgc gtgaggcgct
     2401 acgtgcggtc ccccactcga tcaggctcgg agcgatggca ctaggggcga cccgttggga
     2461 ggccgtttac aagcaggtgc tccccatcgc gatccccggg atcatgacgg ggacgatcct
     2521 cggcctgtca cgtgcgatcg gagaaaccgc gccgttgctg gtcgcaggag cggcggcctt
     2581 cgttgcattc actccggacc accttctgga cgcgtacagc gctctgccca tccagatatt
     2641 cgactgggtt aggaggccac agcccgactt ccaggcgctt gcggccggag caatcctcgt
     2701 actactcgcc ttgttactca ccatgaacgc aatcgcgatc gtgatccgta atcgctacta
     2761 caggagaaat agatgatcgt ggaaacagaa ctggttcgtg atgacatccc gattcccgaa
     2821 gaacaccccg cgcccgtttt ccaggtgcgg gatgtggccg tgacgtacgg cggacaaccg
     2881 gccgttgagg gcatcaccat ggatgtgcct tcagaccgga tcaccgcgct gatcgggccc
     2941 tcaggctgcg ggaagagcac gttgatccgc tgtttcaacc gcatgaacga tctcatcccg
     3001 ggggccgcga tcgccgggag gatcgactac cacggcctcg acctctacgg ccccaatgtc
     3061 gatccggtgg aagtgcgccg aaggatcggc atggtgtttc agcaaccgaa tccctttcct
     3121 aagtcgatct acgagaacgt cgcattcgga ttgcgcgtga acaagatgac agatgatctc
     3181 gacgctcgga tcgagaaagc tcttcgtgac gctgctttgt gggacgaggt caagaggaag
     3241 ctgaagacgc cggcaacttc cctctccggc ggtcaacaac agcgcttgtg catcgcgcga
     3301 gcgctggcaa ccgccccgga cgtgttgctc atggacgaac cctgctccgc cttggatccg
     3361 atcgcgacga ccaagatcga ggatctgatg caccagttga agaccaacta cacgatcctg
     3421 atcgtcaccc acaacatgca acaggcggcg cgggtgtcgg accgaaccgc tttccttacc
     3481 gttcagggct cggaggagga agggatcatc ccccgaggtg tgcttgtcga gttcgacgac
     3541 acggagagga tcttcacgaa tcccagtgac tcacgaacgg aggactacat cacggggagg
     3601 ttcggatgac ggtcagactt aacttc
//
LOCUS       DATMED010000459         2880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4593377, whole genome shotgun sequence.
ACCESSION   DATMED010000459 DATMED010000000
VERSION     DATMED010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4593377"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1158
                     /locus_tag="VNP73_12010"
     CDS             187..1158
                     /locus_tag="VNP73_12010"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HWL66691.1"
                     /translation="MAPTDLKSRPEVQVEHPEPQPIPGVKTLEPRQARFQRRLVLVVT
                     VLPFLGFLAAAWTLWGTGLSVLDASIFLVGYCLTGLGVTVGFHRLFTHKSFEAKPALS
                     AALAIAGSMAIQGPVIRWVSDHRRHHAFADQPGDPHSPHLDEGPGVKGVVKGLWHAHV
                     GWFFDDEQTSARRWAPDLVKDPVMRRIDTLFPLWALISFGAPPVVGFLLTGTLQGAVT
                     AFLWGSLARIFFLHHVTWSVNSICHFYGNRPFKTTDFSTNNWVLAIVSFGESWHNNHH
                     AFPTSAVHGIGKGQVDLSAGLIRLLQKLSLVRDVKVASEKQLANKQA"
     gene            1186..1782
                     /locus_tag="VNP73_12015"
     CDS             1186..1782
                     /locus_tag="VNP73_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWL66692.1"
                     /translation="MTGQERRAQLLDVGRSVFAERGYEASSIEEIAARAKITKPVVYE
                     HFGGKEGLYAVVVDREVQSLLEGITKALAGEDPRTLLEQAAVAFLTYIEDEPEGFRIL
                     VRDSPVAGSTGTLASVIGDIAQQVEHILRDEFKQRGFATKLSPLYSHALVGMVALVGQ
                     WWLDAGDPKKEEVAAHLVNVAWNGLKGMEVDPVLRSSS"
     gene            1828..>2880
                     /locus_tag="VNP73_12020"
     CDS             1828..>2880
                     /locus_tag="VNP73_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWL66693.1"
                     /translation="MAGSRAPLVGRDGELAALEGWLSNADAAGTSAVALIVGEPGSGK
                     TRLLREAVGRLSIDTRLGIEGHESEQAMPFSAASDLMRALTEVPVEGERLGSLLYGTD
                     PHRAEADPLRLFEAARRCLSNLGAVRIVADDLQWVDDQTSSLLRYLLRAARQSGPPLV
                     LLGAARPGTAALSFVGSLARSFNASEEFIQLSVGALSRAEGTELALHLRPDLVEKDAI
                     ELWRKSGGSPFWLTLLAGSRQEAADVAHLMSIRFAGMSADAVLMMTALCVIGRPITVH
                     EVASVLGWRSSRAEQAAMKLGSRELMSEVEGRIVVAHDLIREHAIRSMSTSEILSMRS
                     RVADWLEAQAGDHDIAM"
BASE COUNT          518 a          912 c          896 g          554 t
ORIGIN      
        1 ccttcgttcg cctgactaag ttgagaacat ctcaggcgaa cgtcccgaaa ccgttacgag
       61 gccccgaccg gcggcccggc gatcccgatc accgatttga gcgatttgac tatcaaccta
      121 cgctaacgta agttacgtct acgtaaccgt aaacagttaa cccatcgacg ccgaggagcg
      181 atcaacgtgg cccctaccga tttgaagtcg cgccccgaag tccaagtgga gcaccccgaa
      241 ccccaaccga tccccggcgt gaaaaccctc gagcccagac aggctcgctt tcagcgacgc
      301 ctcgtgctgg tagtgacggt gcttccgttc ctcggattcc tggcggcggc ctggaccctg
      361 tggggtacgg gcttgagcgt gctcgatgcc tcgatctttc tggtgggcta ttgcctcacg
      421 ggcctgggcg tcaccgtcgg cttccaccgg ctgttcaccc acaagagctt cgaggccaag
      481 ccggcgttga gcgccgctct cgccatcgcc ggctccatgg ctatccaggg gcccgtgatc
      541 cgatgggtgt ccgaccaccg tcgccatcac gccttcgccg accagcctgg agatccccac
      601 tccccgcacc tcgatgaggg ccccggagtg aagggagtcg tgaaggggct gtggcatgcg
      661 cacgtcggct ggttcttcga cgatgagcag acctcggcgc gacgctgggc tcccgatctc
      721 gtcaaggatc ccgtcatgcg aaggatcgac accctgttcc cgctgtgggc cctgatctcg
      781 tttggagctc cgccggtggt gggctttctc ctaaccggga cgctgcaggg cgcggtcacc
      841 gcatttctat ggggcagcct cgctcgcatc ttctttctgc atcacgtcac ctggagcgtc
      901 aactcgatct gccacttcta cggcaaccgt cccttcaaga cgacggactt cagcaccaac
      961 aactgggtcc tagcgatcgt gtccttcgga gagtcctggc acaacaacca tcacgcgttc
     1021 cccacctcgg cggtacatgg aataggtaag ggtcaggtcg atctcagcgc cgggctgatc
     1081 agattgctgc agaagctcag tctcgttcgt gacgtgaaag tcgcctccga gaaacaactc
     1141 gccaacaaac aggcgtaaga cgtcgtgagc tcgaggtcgg tccgcatgac gggccaggaa
     1201 cgacgtgcgc agcttctcga tgtcggtcgc tcggttttcg ccgagcgcgg atacgaggcc
     1261 tcatcgatcg aggagatcgc ggcgcgggcc aagatcacca aacccgtcgt gtacgagcac
     1321 ttcggtggga aggaaggcct gtacgccgtc gtggtcgacc gcgaggtgca gagcctgctc
     1381 gaggggatca ctaaggcgct ggcaggcgag gacccgcgca cgctgctcga acaggcggcc
     1441 gtcgctttcc tcacctacat cgaggacgag cccgagggtt tccgcatcct ggtgcgagac
     1501 tcccccgttg ccgggtccac cggcacactg gcgagcgtca tcggagacat cgcccaacag
     1561 gttgagcaca tcctgcgcga tgagttcaaa cagcgcggtt tcgccacaaa gctgtcaccg
     1621 ctttactcgc atgccctcgt cggtatggtc gccctcgtcg ggcaatggtg gcttgacgcg
     1681 ggcgatccga agaaagaaga ggtcgctgct catctcgtca acgtggcatg gaacggactc
     1741 aagggcatgg aggtagaccc cgtgctccgc tcttcctctt gacacgaact aacccctgat
     1801 ctggcggccc gggcgtacgc tcggagcatg gccggatcac gcgctccctt ggtagggagg
     1861 gacggtgaac tcgcagcctt agaaggctgg ctgtcgaacg ccgacgccgc cggcacctct
     1921 gccgttgcgc ttatcgtcgg tgagccgggt agcggcaaga cccggctcct gcgagaagcg
     1981 gttgggcgcc tgagcatcga tacgaggctt gggatcgaag gacacgagtc agagcaggcc
     2041 atgccctttt ctgcggcgag cgatctgatg cgcgcactga ccgaggttcc agtcgagggt
     2101 gaacgcctcg gctcccttct ctacggcacg gatcctcatc gagccgaagc cgatcccctg
     2161 cggctgttcg aagccgcccg tcgctgtctg tcaaaccttg gggccgttcg catcgtggcc
     2221 gatgacttgc agtgggtcga cgatcaaacc tcatctctgc tgcgatatct gttgcgcgcg
     2281 gcgcgtcagt ccggtccgcc actggtcctg ctaggagcgg cgcgacccgg cactgcggca
     2341 ctctccttcg tggggtcgct cgctcggtcg ttcaatgctt ccgaggagtt cattcagctt
     2401 tcggtgggcg ctctgagtcg agccgagggt accgaacttg ctcttcacct tcggcccgat
     2461 ctcgtcgaga aggacgcgat cgaactgtgg agaaagtcgg ggggatcacc cttttggctc
     2521 acgctcctgg ccggaagccg gcaggaggcc gccgatgtcg cgcacctgat gagcatccgg
     2581 tttgccggga tgtccgccga cgcggttctg atgatgacgg cactatgcgt gatcgggcgg
     2641 ccgatcacgg ttcacgaggt cgcctccgtt ctggggtggc gttcatcgcg ggcggagcaa
     2701 gcagcgatga agctcggcag tcgcgaactc atgagtgagg tggagggacg catagtggtt
     2761 gcccacgatc taattcgcga gcatgccatc cgctcaatgt ccaccagcga aatactcagc
     2821 atgcggagcc gagtcgcgga ttggttggaa gcccaagcag gggatcacga catcgcgatg
//
LOCUS       DATMED010000460         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3061575, whole genome shotgun sequence.
ACCESSION   DATMED010000460 DATMED010000000
VERSION     DATMED010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3061575"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VNP73_12025"
     CDS             complement(<1..766)
                     /locus_tag="VNP73_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWL66694.1"
                     /translation="MIRALGRDRSTLVWLGALIALVVASATTAWPAPLGIVFLGAVIG
                     SLISFISIGIVLIYRTNRIINFAQADIGGLAAVLTVLLVAQTGMNYFLGVAIGVLSAM
                     VLGALIELTVVRRFFKAPRLILTVATIAVTQVLVFFELFLPRTFGLDFLVVEFPTPFN
                     VQFAVPDVAGNLPPVIFTGDHVLALLTVPLLIGAIGLFFKFSRFGIASRAAAENSDRA
                     SLLGVPVRRISTLVWILAAGLSAIGALLRAPTIGLSI"
     gene            complement(821..1246)
                     /locus_tag="VNP73_12030"
     CDS             complement(821..1246)
                     /locus_tag="VNP73_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWL66695.1"
                     /translation="MKRLIYLLLAAAVVTLASCGGGGETTPEAQPEPAEEQTTEAMEE
                     EETEAVDVTGEDETDLEVGDFYFEPGALQGEAGQELTIMIHHEGDAPHTFTSDALGVD
                     ESLKPGKEVEVSVTFPDSGSAEFHCEIHPDMTGTIEVAG"
     gene            complement(1308..2822)
                     /locus_tag="VNP73_12035"
     CDS             complement(1308..2822)
                     /locus_tag="VNP73_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWL66696.1"
                     /translation="MGLGAHNLAVLGEKSFEKHGDYESLFFEGTTFTSGELRDRALRL
                     STGFRELGIEPDDRVVVMMSNCPEVGLCYAALWRAGAAITPAIFLLQADDLRHILTHS
                     EARAIVTTPEFLETVKAAAEGVDTLKFIISTGEQTNGIIDLASLEDAQPGDIVPRADT
                     DLAALMYTGGTTGRAKGVMISHENLFYAGKAGHDSGYVPGINRTLIPLPLSHSYGLLV
                     TAVGLHSKEPGFAVLQRWFDGPDFLTQIANHKVQLAAVVPSMLQMLLSLPLEDHDLSS
                     LRFVVSGASPLAAEVIHEFERRVPGAEIREGYGLTETTALVSTTPPGERKIGAVGKLV
                     PGCEVRIVDDEGNDVAGGTEGEIICRSPMVMQGYWRSDDATEDAIKDGWFYTGDIGKL
                     DDDGYLWILDRKKDLIIRGGFNVYPRDIEDVLVQHPAVAIAGVVGKPDPRLGEEVVAF
                     VQLNPGAEATEEELIEFARERLGKYKYPREVRIVDGVPLTPVFKIDRKQLRTLL"
     gene            2885..3301
                     /locus_tag="VNP73_12040"
     CDS             2885..3301
                     /locus_tag="VNP73_12040"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWL66697.1"
                     /translation="MADFRLDHVAIAVHEIEPALKLFRDTFGAKYLFDGPIDAGDGRG
                     SWRWYQMTLPEGGTIELLEPITDGFLTKFLAKRGEGMHHITLKTKDIRAAIDRIQSNG
                     YELVDVNLDNDDWKEAYLRPKQSHGTLIQIAQSKWD"
     gene            3316..>4099
                     /locus_tag="VNP73_12045"
     CDS             3316..>4099
                     /locus_tag="VNP73_12045"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWL66698.1"
                     /translation="MAHFNYDGFKIAYEQRGRKQGASERPIVLIHGLLLPRTHHYPLA
                     DALADRGNRVILIDLLGHGESDQPDHSRFYSMEIFGRQVVGLLDHLDIPEAIIGGTSL
                     GANVTIEVANMAPDRTRGMVIEMPVLERAAPAAGALFLPLVIAYSQASTPFGYFATAM
                     RQLPRGVSLYADVVLDVLSRDPVPSAAILHGLLTGRLAPHPTEREKIEAPTLIMGHER
                     DLLHPFSDAEALHRELRNAELVQANSFFELRFPPNRLSDLICE"
BASE COUNT          795 a         1326 c         1243 g          735 t
ORIGIN      
        1 cgatcgacaa tccgatcgta ggagcgcgca gcagcgcgcc gatcgccgac aaaccggcgg
       61 caaggatcca taccaacgtt gagatgcgcc taaccggaac accgagtaac gacgcgcgat
      121 ccgagttctc ggccgcggca cgcgatgcga tcccgaacct cgagaacttg aagaacaagc
      181 cgatcgcgcc gatcaacagc ggcacagtga gtagagccag aacgtggtcg cccgtgaaga
      241 tgaccggcgg gaggttgccg gctacgtccg gcaccgcgaa ctgcacgttg aacggggtcg
      301 ggaactcgac gacgaggaag tccagcccga acgttcgcgg caggaacagc tcgaagaaca
      361 cgagcacctg agtgacggcg atcgtcgcca ccgtcaggat caggcgcggc gccttgaaga
      421 agcggcgcac caccgtcagc tcgataaggg cccccaggac catcgcgctt aagaccccga
      481 tcgccactcc caggaaatag ttcatccccg tctgggcaac cagaaggacg gtgaggacgg
      541 cggcaaggcc gccgatgtcg gcttgggcga agttgatgat ccggttcgta cggtagatca
      601 ggacgatccc gatagagatg aaagagataa gggagccgat cacggctccc agaaagacga
      661 tccccagcgg ggcgggccag gcggtcgtcg ccgacgccac gaccagcgct atcagcgcac
      721 ccaaccagac gagtgtggat cgatcgcgcc ctaacgccct gatcaaatcg ccccccggtt
      781 attgcccggg gccagtatta cctagagggc ttttttcgtc ctatccggct acttcgatgg
      841 ttcccgtcat gtccgggtgg atctcgcagt ggaactccgc actgccagag tcggggaagg
      901 tcacgctgac ctcgacctct ttgccgggct tgagcgactc atcgaccccg agagcatccg
      961 acgtgaaggt atggggagca tcgccctcgt ggtggatcat gatcgtgagt tcctgacccg
     1021 cctcgccctg aagcgcgccc ggctcgaaat agaagtcgcc gacttccaag tcggtctcgt
     1081 cttcgccggt tacatcgacg gcctcggtct cttcttcctc catagcctcg gtcgtttgct
     1141 cctcggccgg ttccggctga gcttctgggg tggtctcacc accaccccca caggacgcca
     1201 acgtcacgac cgcggccgcg agcaacagat agatgagcct cttcacaagc gcctcccttg
     1261 tcgaccgtcc ctcaggagat atacgccggg aaggccccgg cggattccta gagcagggtg
     1321 cggagttgct tgcgatcgat cttgaagacc ggggtcagcg gcaccccgtc cacgatgcgg
     1381 acctcacgtg ggtacttgta cttgcccagg cgctcgcggg cgaactcgat cagttcttcc
     1441 tcagtggcct cggcccccgg gttgagctga acgaaggcga ccacctcttc gcccaaacga
     1501 ggatcgggct tgccgacgac cccggcgatc gcgaccgccg gatgctgaac gagcacgtcc
     1561 tcgatgtcgc gcgggtaaac gttgaaccca ccgcggatga tcaggtcttt cttgcggtcg
     1621 aggatccaga ggtagccgtc atcgtcgagc ttcccaatgt cgcccgtgta aaaccaaccg
     1681 tctttgatcg cgtcttcggt tgcgtcgtcg gatctccagt acccctgcat caccatcggg
     1741 gaacgacaga tgatctcgcc ttcggtccca ccggcgacgt cgttgccctc gtcgtccacg
     1801 atgcgcacct cgcaaccggg aacgagtttg ccgaccgcgc cgatcttgcg ctcgcccggc
     1861 ggcgtcgtcg agacgagcgc agtcgtctct gtcaacccgt acccctcgcg gatctcggca
     1921 ccgggaacgc gacgctcgaa ctcgtggatc acttctgcgg ccagcggaga cgcacccgat
     1981 acgacgaaac gcagcgatga gaggtcgtga tcctcgagcg gcagcgacag cagcatctga
     2041 agcatcgagg gaacgacggc cgcaagttgg accttgtggt tcgcgatctg agtcaagaag
     2101 tcgggcccgt cgaaccagcg ctgcagaacg gcgaagccgg gctccttcga gtgcaacccg
     2161 acggcagtaa cgagcaatcc gtaggagtgc gacagcggca acgggatgag agtgcggttg
     2221 atccctggga cgtacccgga atcgtgtccg gccttgcccg cgtagaacag gttctcgtga
     2281 ctgatcatca cgcccttggc gcgaccggtc gtgcctcccg tgtacatcag cgcggcgaga
     2341 tcggtgtcgg cgcgcggcac gatgtcgccg ggctgtgcat cctcgagcga ggcaaggtcg
     2401 atgatcccgt tcgtttgctc gccggtggag atgatgaact tcagggtgtc gacgccttcg
     2461 gctgccgcct taacggtttc gaggaactcc ggggtggtca cgatcgcgcg ggcttctgag
     2521 tgggtgagga tgtgtcggag atcgtcggct tgcagaagga atatggccgg tgtgatcgcg
     2581 gcccccgccc tccataaggc cgcgtagcag aggcccacct cgggacagtt ggacatcatg
     2641 acgacgaccc ggtcatccgg ctcgataccc aactcccgga acccggtgga caggcgaagc
     2701 gctcgatcgc gcagttctcc cgaggtgaac gtcgtcccct cgaagaacag cgactcgtag
     2761 tcgccgtgct tctcgaaaga cttctccccc aataccgcga ggttgtgcgc ccccaaaccc
     2821 atctggtccc tccccgtctt agagtcctcg ttcctagttt aggcatttcg actttggagg
     2881 ttctatggcc gatttccgtc tcgaccacgt tgcgatagcc gtccacgaga tcgagcccgc
     2941 cctgaagctg ttccgggaca ccttcggggc caaatacctg ttcgacggcc cgatcgacgc
     3001 cggcgacggc cggggatcat ggcgctggta tcagatgacg ctgcccgagg gaggcacgat
     3061 cgagcttctc gagccgatca cggacggatt cctaacgaag tttctcgcca agcgcggtga
     3121 gggtatgcat cacatcacct tgaagacgaa agacatccga gccgcgatcg accgtatcca
     3181 gtcgaacgga tacgaactgg tcgacgtgaa cctcgacaac gacgactgga aggaggcgta
     3241 cctgcgcccg aagcaatcgc acggaacgct gatccagata gctcagtcga agtgggactg
     3301 acttaggctc gcatcgtggc tcatttcaac tacgacgggt tcaagatcgc ctacgagcag
     3361 cgcggacgca agcaaggggc gtcggaacga ccaatcgtgc tgatccacgg tttgctgctg
     3421 ccgcgcaccc atcactatcc gctggccgat gcactcgccg atcgcggcaa ccgggtgatc
     3481 ttgatcgacc tcctcgggca cggcgagagc gaccagcccg atcactcccg cttctattcg
     3541 atggagatct tcggacggca ggtcgtgggc ttgctcgatc acctcgacat ccccgaagcg
     3601 atcatcgggg gaacgtcgct cggcgccaac gtgacgatcg aggtcgccaa catggctccc
     3661 gatcgcaccc gcgggatggt gatcgagatg ccggtgctcg agcgagcagc tcccgcggcc
     3721 ggggctctgt tcctccccct ggtcatcgct tactcgcaag cctccacacc gttcggctat
     3781 ttcgctacag cgatgcgaca gttgccgcgt ggggtctcgc tctacgcgga cgtggtcctc
     3841 gacgtgttgt cgcgcgatcc cgtcccgtcc gcagccattc ttcacggcct gctcaccgga
     3901 cggctcgcac cccacccgac cgaacgggaa aagatcgagg ctccaacttt gatcatggga
     3961 cacgagcgtg acctactaca tcccttctcc gatgcggagg cactgcatcg cgagttgcgc
     4021 aacgccgagc tcgtccaggc gaactcgttc ttcgagctgc gcttcccgcc gaaccggctg
     4081 tcggacctga tctgcgaat
//
LOCUS       DATMED010000461         4339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_756822, whole genome shotgun sequence.
ACCESSION   DATMED010000461 DATMED010000000
VERSION     DATMED010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756822"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VNP73_12050"
     CDS             complement(<1..1603)
                     /locus_tag="VNP73_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWL66699.1"
                     /translation="METFERSTEILTAALFAAIALIGAWRGRRGARASRRAGLAFGSL
                     AFVLVLSLLPDTALEPLGSWGGKVIILTLFAFPYLLFRFTEAFHDYPPLIKRAALAGL
                     LIVAATTTAIPITADESESPGMLGYLVVVLFYWTFVSSVTGIQLWGAGRGQPSLARKR
                     MRLMSVATLGLNLVLIFGVVPGGDSNTASVIVQIAVLVTGVSFLLGFSPPRILRYAWR
                     EPEETALREAVQQLMKADTLQEVSDLLLPHALALVGASTVELRGPDGEVLASLGAPSG
                     AGTSGDFEEGAHTDLPLTAPNVSILRVWTSRYTPFFGRDDVEALSGLAAVADLAFERC
                     RLFEVERELRATADASREEAERANLAKSEFLARMSHELRTPLNVILGFAQILETSDLI
                     EEDREAVAHMIKAGHHLLELINEVLDLSRIESGRLSISVEPVQADDIVRESLSLIQPV
                     AAERGITIHEDLSECDVFVHADRQRSKQVLLNLLSNAVKYNRDKGQIIVSGGRSGDRL
                     FVTVSDTGPGLPEGSADKLFQPFERL"
     gene            complement(1923..2291)
                     /locus_tag="VNP73_12055"
     CDS             complement(1923..2291)
                     /locus_tag="VNP73_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWL66700.1"
                     /translation="MGEALKVLVVDDDAAMRKMLRRLLSSAASVEDIQEAGSGGEAIE
                     MLPSFSPDVVIMDAHMPGMNGAEATRQIKERFAHIIVIGVSGTDDHSMREAGATTVLL
                     KGDVTTALVELLESMAGSTE"
     gene            2315..3058
                     /locus_tag="VNP73_12060"
     CDS             2315..3058
                     /locus_tag="VNP73_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66701.1"
                     /translation="MRPSALALGMLAAVAASLFAMGQRPVGAGSADCNWNRIPTTPTP
                     SPSTTVLDQSNFVDRDIDGVPDRWGSSTDYTRDECRRVTGPRGGDSLVKQVIRPTATN
                     PYADSDESADVSIQRVYSAAPGQIYTATADVDIANYPWTGNEEDDFRGRLKLQPCTSV
                     PCRFDQFEVEYIANLCISEEQDHDTTGSELCVEYDEQTTGWKTLTLTTPPLPEGTKYL
                     LLAWRVREFASDSAWGNGRLTRIVLYRNE"
     gene            3200..4288
                     /locus_tag="VNP73_12065"
     CDS             3200..4288
                     /locus_tag="VNP73_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66702.1"
                     /translation="MGKTFSARRILVALTSTIALMAIVPTPASAQFASGLQPEDAFNH
                     YLTASADGERLYSAATNCDSLPPPCDQVLFESTDGGTTWEQLPARLFDGHKILIPPDA
                     ADQRIFALAAGNLQVSEDGGQTFRPTAPAFQAVSAAISPAFNEGDPTFLIGGTPTTMY
                     NDETHVVTPLPGVLSVGPSNPAFSPGYPDDSRLFFGHNVLGDEPNTIKGVVTVCVDHL
                     VSCSTTEVGTFGPEIRFAPDFAEGGALYAFDQRGVHVSNDGGETFEDITPPERATIWD
                     FEVLDDGSLLVARTFNPFQDPGAEGLFRSTDGGVSWTRVSSPMLDVGVFSLAAGGDTV
                     VAALTSPGLGDEVVCSDDGGLTWASRCP"
BASE COUNT          910 a         1409 c         1257 g          763 t
ORIGIN      
        1 ccagccgctc gaagggttgg aacagcttgt cggcacttcc ttcagggagg ccgggcccag
       61 tatccgaaac ggttacgaac aggcgatcac ctgagcggcc gcccgacacg atgatctgtc
      121 ctttatcgcg gttgtacttg accgcgttcg acagaaggtt gagcagaacc tgcttcgagc
      181 gttgtcgatc cgcgtgaacg aagacatcgc attcactgag gtcttcatgg atggtgatgc
      241 cacgctccgc cgcaacgggt tggataagac tgaggctctc acggacgatg tcatcggcct
      301 ggacaggctc aacggagatc gacagtcgcc cggactcgat gcgggacagg tccaggacct
      361 cgttgatcag ctccaagaga tggtggccgg ccttgatcat atgcgcaacc gcttctcgat
      421 cctcttcgat cagatccgat gtctcgagga tctgagcgaa tcccaagatc acgttgaggg
      481 gggtgcggag ctcgtggctc atccgcgcga ggaattcact cttcgcaagg ttcgccctct
      541 ccgcttcctc gcgactggcg tctgctgtgg cccgcaactc gcgttcgact tcgaacaatc
      601 gacatcgctc gaacgcgagg tcggcgacgg ctgccagccc cgatagagcc tcgacgtcat
      661 cgcgaccgaa gaagggcgtg taccggctcg tccatacccg caaaatcgac acattggggg
      721 ccgtcaaggg gagatctgtg tgagcgccct cttcgaaatc accggaagtc ccggcccccg
      781 acggagctcc cagcgaagcg agaacttcgc cgtccggacc cctaagttcg accgtagaag
      841 caccgaccag agcaagagcg tgtggcagta ggagatcact gacctcctgc aacgtatctg
      901 ccttcatgag ctgctgcacg gcttcgcgta atgccgtttc ttcaggctcg cgccacgcgt
      961 aacgcaggat tctcggtggg ctaaaaccaa gcaggaagga aacgccggta acaagaacgg
     1021 ctatctgtac gatcacggaa gctgtgttgg aatcaccgcc gggaaccacg ccgaagatca
     1081 gcaccaggtt caacccgagg gttgcaacgc tcatgagccg catgcgcttc cgggccaaac
     1141 ttggctggcc gcgtcccgcg ccccagagtt ggatccctgt gaccgaggaa acgaacgtcc
     1201 agtaaaacaa cacgaccacc agatagccca gcattccagg cgactcggat tcgtcggccg
     1261 ttatcgggat ggcggttgta gttgcggcca ctattaggag tccggccaag gctgcccgct
     1321 ttatcagcgg cggatagtca tggaaggcct cagtgaaccg aaacagaagg taagggaacg
     1381 cgaagagcgt caggatgatc accttgccgc cccaagatcc aagtggctcg agggcggtgt
     1441 ccggcagtaa ggacaggacc aggacgaacg ccaacgatcc aaacgcgagc ccggcccgac
     1501 ggcttgcccg ggccccacgg cgaccgcgcc aagctcctat cagggcgatc gccgcaaaaa
     1561 gggcggccgt taggatctcg gtcgatctct cgaaggtctc catcagtcct cctgaaccac
     1621 cgggcatccc cggcctgtcg gttcctcgaa ccgatgaccc ccccgggtca atcgagtatg
     1681 cccagccaca atgaggaaac cattaagaaa ccctgagaag agaccccagg aagagcgccc
     1741 tagttgcaac cggggtgact gcgccagacc ttctcgtatc tcgggataga cgtactggaa
     1801 tgttctgtca atgaaatcgc aagcgaatcc cttcaacgag tcagagaatt cctcgtttga
     1861 gacttgacgt aaccagatgt ggttaggaaa gaggtagtgc gttagtactc agtacgtgag
     1921 tactactccg ttgatccagc catgctctcg aggagctcaa cgagcgcagt cgtgacgtcc
     1981 cctttcagaa ggacagtagt ggcgcccgct tccctcatcg aatgatcgtc ggttccggac
     2041 acgccgatca cgatgatgtg agcgaaacgt tccttgatct ggcgagtcgc ctcagcgccg
     2101 ttcatccccg gcatgtgtgc gtccatgatg acgacgtcgg gggaaaacga agggagcatc
     2161 tcgatcgctt ccccgcccga gccggcctcc tgaatgtcct ccactgaagc ggcagacgac
     2221 agaagccggc gcagcatctt tctcatcgcc gcgtcatcgt ccaccaccag cactttcagt
     2281 gcctctccca catactcagg ataaacatag agagatgaga ccttcggcgc tcgccctggg
     2341 catgctcgcc gccgttgccg cgagcctttt cgccatgggg caacgacccg tcggtgcggg
     2401 atccgccgac tgcaactgga accgcatccc caccaccccg acgccgtcgc cctccaccac
     2461 cgtgctcgac caatccaact tcgtcgatag agacatcgac ggcgtgcccg atcgatgggg
     2521 ttcgtccacg gactacacgc gcgacgagtg tcgtcgggtc accggccccc gcgggggcga
     2581 cagtctcgta aagcaagtca tccgcccgac cgccaccaac ccgtatgccg acagcgacga
     2641 gagcgccgac gtgtcgatcc agcgtgtcta cagcgcggcc cccggccaga tctacaccgc
     2701 gacggcagac gtcgacatcg ccaactaccc gtggacgggg aacgaggagg acgacttccg
     2761 tggacgattg aagctccagc cctgtacatc ggtcccctgc cgcttcgacc aattcgaggt
     2821 cgaatacatc gcgaacctct gcatcagcga ggagcaagac cacgacacca ccggaagcga
     2881 actgtgcgtc gaatacgacg aacagacgac gggctggaag accctgactc tcacgacccc
     2941 tccgctcccc gaaggcacca agtacctgct gctcgcgtgg cgggtgcgcg aatttgcatc
     3001 ggattcggcg tggggcaacg gccgactaac gcgtatcgtc ctctacagga acgagtgacc
     3061 agtcagaaga cccatttgtc cgtttcgtca caaacaagcc gagcaaatcc cgtgccccgc
     3121 ataggagttc cccgtacggc gccgaatccg tagcccaact ccgtcaacga acggcaacca
     3181 ctactccagg ggggttcgga tgggcaagac cttttctgcg cggcgcatcc tcgtcgcgct
     3241 cacgagcacg atcgccctga tggcgatcgt ccccacgcct gccagcgcgc aattcgccag
     3301 cggcctccaa ccggaggatg cgttcaatca ttacctgacc gcgtcggcag acggcgagcg
     3361 cctctactcc gccgcgacga actgcgactc cctgccgccc ccctgcgacc aggttttgtt
     3421 cgaatccacc gacggtggaa ctacatggga gcaactgccc gcacgcttgt tcgatggcca
     3481 caagatcttg atcccgcccg atgcagcgga ccagcgcatc ttcgccctcg ctgcgggcaa
     3541 ccttcaggta agcgaagacg gagggcagac cttccggccg acggcgccgg ctttccaggc
     3601 cgtctcggcg gcgatctcgc ccgccttcaa cgagggagac ccgaccttcc tcatcggtgg
     3661 aacaccaaca acgatgtaca acgacgaaac acacgtcgtt acgccactac cgggagtgct
     3721 cagcgtcgga ccgagcaacc ccgccttctc gccgggatac cccgacgact cacgtctttt
     3781 cttcggccac aacgtgctgg gtgacgaacc gaacacaatc aagggcgtcg tcacggtctg
     3841 tgtcgaccac ctagtgagct gctcgaccac cgaggtcgga accttcggcc cggagatcag
     3901 gttcgccccc gacttcgccg aaggcggagc gctgtacgcg ttcgatcaga ggggcgtcca
     3961 cgtgtcgaac gacggcgggg agaccttcga agacatcaca cctccggagc gtgcaaccat
     4021 ctgggacttc gaagtgctgg acgacggctc cttgctcgtg gcgcgcacct tcaatccgtt
     4081 ccaagacccc ggggcagaag gtttgttccg ctcgaccgac ggaggagtca gctggaccag
     4141 ggtgagcagc cccatgctgg acgttggcgt cttctcgctg gcggccggtg gcgacaccgt
     4201 agttgccgca ctgacctctc cgggtctggg cgacgaggtg gtctgctctg acgacggtgg
     4261 tctgacctgg gcgtcgcgct gtccgtgatc cagacgggcg acccggagag tagttagttc
     4321 ttaactcggg acctactgc
//
LOCUS       DATMED010000462         3130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_758405, whole genome shotgun sequence.
ACCESSION   DATMED010000462 DATMED010000000
VERSION     DATMED010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758405"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VNP73_12070"
     CDS             complement(<1..1262)
                     /locus_tag="VNP73_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66703.1"
                     /translation="MEPVELLLAVAQAVLFLLVGLVLSERFLLRLDAGMEVGGPERLL
                     FGIVGGVGFAASLMVGNIITGGAVFGLPFFVPIVAALCLLVGWKALRVPRGIQWAPLI
                     VITVALVLLFAVPSMAGGSVIRTGDSPWHLGWSQQLLGGEPIPSGPAAGYGRNAYPWG
                     WHALTATLVRLVPGSTIVTAYSALQLLLLFALPLAGACLSRRLDPAAGWPGAIAMSLV
                     GGFGWVTAQGAAFVATPAHSRFGADLVVASPNSVYELFAPPLPRELGVVLLAATATMM
                     VLALRQGGRTHALAAGVCAGLLGLVSVPLFLSAIVWFAIVSMLGIGKRGQIGFALLPA
                     FVIFLLWAGPVLANYAAYGGFVNITPSLGKEWDLVTALQSWGFLFPLAMIGVWVALKN
                     PSSRPMVAFGIGTAMMLGAASARASFDWT"
     gene            complement(1262..2950)
                     /locus_tag="VNP73_12075"
     CDS             complement(1262..2950)
                     /locus_tag="VNP73_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006181112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWL66704.1"
                     /translation="MFSKILVANRGEIAVRIARTCKERGVAYVAVHSDVDAKARHVAV
                     ADEAVHIPGVAPNETYLNVEAILDAAARAGAEAIHPGYGFLSENADFAEAVTAAGLVW
                     VGPPPEATRAVGDKIQARRLATSAGVPIVPGTTEPLGSVDEVRTFADEHGYPVAIKAS
                     GGGGGRGLKVARGPDDLEQAFESSRREAQSYFGSDEVYIERYLENPKHLEVQLLAPNP
                     DEAMWLGVRDCSLQRRHQKLIEETPPPRFAERSADMGLAAISLSKACGYVNAGTVEML
                     VDEDGSFYFLEVNARLQVEHTVTEETLGLDLVACQLQIASGDALGFSQQDIEAHMRGH
                     SIECRINAEDPSAGFVPSPGRITGYVEPNGLGVRVDSGYTAGDEIPGAYDSLVAKLIT
                     WGNDREEARVRMLRALDEFVIEGVATTIPAHKVLLTDDVFVRGEHTTRTVEGSGVLDA
                     LAGDEGDEQPQDVLMVAGRPVRLWHSGMSKSAAAATHGAFGGSGDVTVPMQGTVLKVL
                     VAEGAEVAQGDPVIVLEAMKMETTVVATRAGVVTEVRVDAGENVAAGEVIAVIE"
BASE COUNT          623 a         1089 c          939 g          479 t
ORIGIN      
        1 gtccagtcga acgaagcgcg agccgaagcc gcaccgagca tcatcgcggt gccgatgccg
       61 aacgccacca tcggcctact cgatgggttc ttcaacgcaa cccaaactcc gatcatcgcc
      121 aacgggaaga ggaatcccca cgactgcaac gcggtgacca gatcccattc cttcccgagt
      181 gaaggtgtga tgttcacgaa tccgccgtac gccgcatagt tcgccagcac cggtcccgcc
      241 cacaacagaa agatcacgaa cgccggcaac agagcgaagc cgatctggcc ccgtttgccg
      301 atgccgagca tggagacgat ggcgaaccaa acgatcgcac tcaagaagag gggcacgctc
      361 acgagaccca gtagcccggc acacaccccc gccgcgagtg catgcgtgcg tcctccctgg
      421 cgcagcgcca gcaccatcat ggttgcggta gcagcgagca gaacgacgcc gagctcgcgc
      481 ggcaatggag gagcgaacaa ctcgtagacc gaattcggag acgccaccac cagatcggca
      541 ccgaagcgcg agtgagccgg cgttgccacg aacgcagcac cttgagcggt cacccatccg
      601 aaccctccga ccaacgacat ggcgatggct cccggccatc cggcggcggg atcgagacga
      661 cgagagagac acgctccggc aagcggcagc gcgaacagca acagcaactg cagtgccgaa
      721 taagccgtca cgatcgtcga gccgggtacg agtctcacga gcgtggcagt caatgcgtgc
      781 cacccccagg ggtacgcatt cctgccgtag cccgcggccg gtccgctggg gatcggctcg
      841 cctcccaaca gctgctggct ccaccccagg tgccaggggc tgtcgcccgt gcggatgacc
      901 gagcccccgg ccatcgaggg gacggcgaac agaagtacga gcgcgaccgt aatgacgatg
      961 agaggtgccc attggatccc acggggcacg cgaagggcct tccacccgac gagcaagcac
     1021 agggcagcga cgatcggaac gaagaacgga agaccgaaga ccgccccacc ggtgatgatg
     1081 tttccaacca tcaaggaggc cgcgaacccg accccaccca cgatcccgaa cagcaggcgc
     1141 tcggggcccc caacctccat ccccgcgtcc aatcgcaaca ggaatcgctc cgagaggacg
     1201 agcccgacga gcaggaacag cacggcctgg gcgaccgcca gcaacagctc gaccggttcc
     1261 attactcgat cactgcgatc acttcgccgg cagccacatt ctccccggcg tcgaccctca
     1321 cctcggttac gacccccgcc cgcgtcgcga cgacggtcgt ctccatcttc atcgcctcga
     1381 gcacgatgac cggatcgccc tgcgcgacct cggcgccctc ggcaaccagc accttgagca
     1441 cggttccctg catcggcacg gtgacgtcac ccgaccctcc gaatgcgccg tgcgtcgcgg
     1501 ccgcggccga cttcgacatc cccgaatgcc acaagcgcac cggacgaccg gcgaccatca
     1561 acacgtcctg gggttgctcg tctccttcat caccggcgag agcatcgagg accccactgc
     1621 cctcgacagt gcgcgtggtg tgctccccac ggacgaagac atcgtcggtc agcaacacct
     1681 tgtgagccgg gatcgtcgtt gccacgcctt cgatgacgaa ctcgtccaga gcgcgcagca
     1741 tccgcacgcg cgcttcctca cgatcgttgc cccatgtgat gagcttcgct accagcgagt
     1801 cgtaagcacc ggggatctcg tctccggccg tgtagccgga gtcgacccgg acaccgagtc
     1861 cgttcggctc gacgtaacca gtgatccggc cgggcgaagg aacgaagccg gcggacggat
     1921 cctccgcatt gatccggcat tcgatcgagt gcccgcgcat gtgcgcttct atgtcctgct
     1981 gtgagaatcc aagagcgtcc cctgacgcga tctgcaactg acatgcgacg aggtcgagcc
     2041 caagcgtttc ctccgtaacg gtgtgctcta cctgcaaccg cgcattgacc tcgaggaagt
     2101 agaaggagcc gtcctcgtcg acgagcatct cgaccgtccc tgcgttgacg tagccgcacg
     2161 ccttcgagag tgagattgca gcgaggccca tgtcggcaga tcgctcggcg aagcgcgggg
     2221 gcggcgtttc ttcgatcagc ttctgatggc gacgctgaag cgagcagtcg cgcactccca
     2281 gccacatggc ctcgtcgggg ttcggggcca ggagctgaac ctcgaggtgc ttcgggttct
     2341 cgaggtaacg ctctatgtag acctcgtcgg atccgaagta cgactgagcc tctctacgtg
     2401 aggattcgaa ggcctgctcg agatcgtccg gcccccgagc caccttcaga cccctaccgc
     2461 caccacctcc ggaggctttg atggccaccg ggtatccgtg ctcgtcggcg aaggtgcgta
     2521 cctcgtccac cgatcccaga ggctccgtgg ttccgggaac gatcgggacg cccgcgctcg
     2581 ttgccaggcg acgggcttgg atcttgtcac caacggcccg cgttgcttcc ggcggaggac
     2641 cgacccacac caaacccgct gcagtgaccg cttcggcgaa gtcggcattc tcggagagga
     2701 acccgtaacc gggatggatc gcctcggcgc cggcgcgcgc ggccgcgtcg aggatcgcct
     2761 cgacgttcag ataggtctcg ttcggggcga cccccggtat gtgaacggct tcgtctgcga
     2821 cggcgacatg gcgcgccttc gcgtcgacat ccgagtgaac ggccacgtaa gcaaccccgc
     2881 gctccttgca cgtgcgggcg atgcgaaccg ctatctcgcc acgattggcg accaggatct
     2941 tggaaaacat ctcagcttgt ggagccgggg acctgcgacc gggggccggc ccgcaagacc
     3001 ttgctgggga ggtcgcccac gatcaaccct tgggcgatgc gcgcggcctc gagcaagtgt
     3061 tttaggtcaa caccgatgtc gatccccatg tcatcgagca tatgcaccag atcctcagtc
     3121 acgatgttgc
//
LOCUS       DATMED010000463         5840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4601117, whole genome shotgun sequence.
ACCESSION   DATMED010000463 DATMED010000000
VERSION     DATMED010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4601117"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VNP73_12080"
     CDS             <1..755
                     /locus_tag="VNP73_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWL66705.1"
                     /translation="AGLIRSDSILVAGMAVNNEIGVVQDVGTLVAAVKERNPSTLFHT
                     DAVQALGNIPVDVHSWGVDLAAFAGHKIGGPKGVGALYVRSGVPLEPVIHGGGQERGL
                     RSGTLNVPGIVGLGIAAEIAAREVDAKAARLRTLRDGLLDGIRRAIPDVVVNGTLEHR
                     APNNVNVCIPGTEGETLLLLLDERGIACSSGSACASGALDPSHVLLALGLQPEVAKGS
                     LRFTLGRSSTKADVDALLDVLPDVVGRARKVA"
     gene            752..1855
                     /gene="mnmA"
                     /locus_tag="VNP73_12085"
     CDS             752..1855
                     /gene="mnmA"
                     /locus_tag="VNP73_12085"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWL66706.1"
                     /translation="MSKKAVVAMSGGVDSSVAAALLVEQGYDVTGVMLKLWRGEAENN
                     NSGCCNLGAAEDARRVADVLGIPFYVFNFAEDFERSVIADFHATYAAGRTPNPCVRCN
                     QWIKFETLLDRARQLGADLLATGHYARVIKDGDRYALKRGVDKSKDQSYVLWMLTQDE
                     LAHVAFPVGDQVKSRTRALAAQLGLRTAAKPDSQEICFVRNGDVGAYLDQHVGRKPGE
                     IVDADGTVLGRHDGIGRFTIGQRKGLGISVGMPRFVSHIDAVGGRVTVGSRADLAVAA
                     FSAEELHLVDADLPPGSGIFIQHRAHGEVNPGEVVARGRDRIEIEFDTSVEAVAPGQS
                     AAFYSTDDPDLLLGGGIISETASAVRTRGVVAS"
     gene            1852..3606
                     /locus_tag="VNP73_12090"
     CDS             1852..3606
                     /locus_tag="VNP73_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66707.1"
                     /translation="MIKRVGIVVAAVSLILVAVPAGKTQPAPPASCGLTTIQGTLDYS
                     GFEEGLALACGPGQPLVVRTKFLDASKKRLKNRKVLASFITIADVQLADEESPLRAEW
                     ADKCEEHPATAAFRPHETMVPHLTNAHIRAADAIAEKGSPVMKADFDFAIGLGDLADN
                     NQYNEIRWIIDLFDGDKMIDPDTGDEGYDGVQSADPEGAPNAEADVVSPVEGTTMLEL
                     ANEPFWAVGLRAGGKSLPWYSLPGNHDIKIQGTAPNEAGWRDFASQYAVGGVKFTDVP
                     PDKQQEACQAGFSEEFFTEMATNPGAAEPVPADPDRRLLSREEWAQEHLTTTGVPEGH
                     GYGGERCKDDAGELLERLCYSWTEGDFHYIGLDTNPHEGLEDGNIDDPQFQWLEKDLQ
                     AHSSTYYTAEGLRKKNPSGDDKLIVVFAHHPQQSMTNTSTEGSHQAQDLKQLLLRFPN
                     VIVMANGHTHQNRIWGRINKKAKTGYWEVNTSAIADFPSQSRTVEIVKNGDGTLSIFA
                     VVFDAAVAPDVRSMDWADDPTNEVDLGGAETAINEDWLASYGREVMLYDPQQDLMKLG
                     TPKDRNVELLLRAPRWFR"
     gene            3607..4365
                     /locus_tag="VNP73_12095"
     CDS             3607..4365
                     /locus_tag="VNP73_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWL66708.1"
                     /translation="MSDSDREHTKEVTEQFTRAAEAFSERSRGRFDVLDPVAFSRVEP
                     GQSVIEVGGGSGNFISKFQSIAGLAAIFDLTPAMLGVARRTYPGLALVVADAERLPLA
                     SGSADLATTAQALHHMATPVEVVRELRRVTKRSGRVLVVDSVAPDRIEEARAMNELDL
                     IRDPSHVAFRSPAAMKVIVQAAGLDIVDEHLSVEQQKLSQWMWPDEFPGERIEAVRNF
                     IEERGAETGMKFERSGGDWVFERRRLMLLCERAK"
     gene            4375..>5840
                     /gene="ligA"
                     /locus_tag="VNP73_12100"
     CDS             4375..>5840
                     /gene="ligA"
                     /locus_tag="VNP73_12100"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWL66709.1"
                     /translation="MAAPTKAKAKKRVAELRDAIDYHSYRYHALDDPEIADAEYDALV
                     GELIELETEYPDLITPESPTQRVGAPPSDLFAAAEHLSPMMSLDNCFSLEELQAWGKR
                     VERGIGKADGFVVELKMDGVAVNLIYEKGVLVRAATRGDGRVGEDITANVKTIRAVPL
                     KLRGKPPEIIEVRGEIYMRTDDFEKLNSMLGEQGHKTFANPRNGAAGSLRQKDPTVTA
                     QRNLSLVCHGTGQIQGLRFPRHWGALETIRELGLRTNPENKQVETLDDVHAFCRHWEE
                     QRHDVPYEIDGVVVKVDSIAQQEELGWTSKAPRWAIAYKFPPEERTTVLKRIEASVGR
                     TGVVTPFAQLETVFVGGVNITTATLHNEDEVARKDVRQKDTVIVRRAGDVIPEVVGPV
                     LSKRPKGTKRWKMPRKCPSCGSELVRAEGESATRCLNIYECSAQRRERIFHFASRGGM
                     DIEGLGVQTIVALIEQEWLKDVSDIYFLTREQVLELEG"
BASE COUNT         1155 a         1702 c         1928 g         1055 t
ORIGIN      
        1 tcgccggcct gatccgctcc gactcgatcc tggtggcggg gatggccgtg aacaacgaga
       61 tcggcgtcgt gcaggacgtc gggacgctgg tggccgcggt caaggaacgc aatccctcca
      121 cgctttttca cacggacgcc gttcaggccc tcggcaacat ccccgtggac gttcattcgt
      181 ggggtgttga ccttgctgca ttcgccggac acaagatcgg tgggcccaaa ggggtaggcg
      241 ctctgtacgt tcggtcggga gtgccgctcg agccagtgat ccacggaggc ggacaggaga
      301 gaggtcttcg atcggggacc ctcaacgttc ccgggatcgt gggcctcggt atcgcagccg
      361 agatcgcggc gcgggaagtg gatgcgaaag cggccaggct gagaaccctg cgggatggcc
      421 tgctggacgg catccgcagg gcgatccccg acgtggtcgt caacggcacc ctggagcacc
      481 gtgcccccaa caacgtgaac gtttgcatcc ccgggaccga aggggagacc cttctgctac
      541 ttctggatga acgcggtatc gcttgttcgt ccggttcggc atgtgcctcg ggcgcgctcg
      601 atccctcaca cgttctgctg gcgctcgggc ttcagccgga ggtcgcaaaa ggaagcctca
      661 ggttcaccct cggtcgctcc tcaacgaaag cggatgtgga cgcgcttctg gatgtgttgc
      721 ctgatgtggt cggccgggcc aggaaggtcg catgagcaag aaagcggtcg tcgcgatgtc
      781 gggcggtgtc gattcctccg ttgcagcagc tttactcgtc gaacagggct atgacgttac
      841 cggcgtgatg ctgaaactgt ggcgcgggga agctgagaac aacaactcgg gttgctgcaa
      901 tctcggagcg gccgaagatg ctcgccgggt cgccgacgtg ctggggatcc cgttctacgt
      961 ctttaacttc gccgaggatt tcgagcgttc tgtgatcgcc gatttccacg caacgtacgc
     1021 cgcgggacgc actcccaatc cctgcgtcag atgcaatcag tggatcaagt tcgagacgct
     1081 tctggatcgc gcgagacaac tgggggccga cctacttgcg accggccatt acgcgcgggt
     1141 catcaaggat ggcgatcggt acgccttgaa gcgtggcgtc gacaagagca aggatcagtc
     1201 gtacgtcctg tggatgctga cccaggatga gcttgctcac gttgcctttc ccgtcggtga
     1261 ccaggtgaaa tcccggaccc gggccctcgc tgcccagctg gggcttcgca ctgcggcgaa
     1321 acccgactcc caggagatct gcttcgttcg gaacggcgat gtaggtgcct acctcgacca
     1381 acacgtcggc aggaagccgg gggagatcgt cgatgccgac ggcactgtgc tgggcaggca
     1441 cgacgggatc ggccgtttca cgatcgggca gcggaaaggg ctcggtatct cggtcggaat
     1501 gccccgtttt gtctcgcaca tagatgcagt cggcggacgc gtcaccgtcg ggtcgcgagc
     1561 cgatctagcc gttgcggcat tttcggctga agagctgcac ctcgtggatg ccgacctccc
     1621 gccgggatcc gggatcttta tccagcatcg ggcgcacggc gaggtgaatc cgggtgaggt
     1681 cgtcgcacgt ggtcgggacc ggatagagat cgagttcgac acatcggtgg aagctgttgc
     1741 ccccggtcag tcggccgcct tttattcgac cgatgatccc gacctgttgt tgggaggtgg
     1801 catcatttcc gagacggctt cggctgtccg gacgagggga gtagtcgctt catgatcaag
     1861 cgcgtgggga tagtcgtcgc ggcggtgagc ttgatcctcg tggcggtccc ggcgggcaag
     1921 actcagcctg caccaccagc ctcgtgtgga ctcacgacca tccaggggac cctcgactac
     1981 tcgggcttcg aggaggggct ggcgttggcg tgtgggcccg ggcaaccgct cgtcgtcagg
     2041 accaagttcc tcgatgcatc gaagaagcga ctgaagaacc ggaaggttct ggcgagcttc
     2101 atcacgatcg ccgacgtcca gcttgccgac gaggaatctc cacttcgggc ggagtgggcg
     2161 gacaagtgtg aagaacatcc tgcaaccgca gcgttccgcc cccacgaaac gatggtgccc
     2221 catctgacga acgcgcacat ccgagctgca gacgcgatcg ccgagaaagg cagtcccgtc
     2281 atgaaagccg actttgactt cgcgatcggg ttgggggatc tcgcggacaa caaccagtac
     2341 aacgagatcc gctggataat cgatctcttc gacggcgaca agatgatcga tcctgatacg
     2401 ggggatgagg gatacgacgg cgtccaatcg gcggatccgg agggtgctcc gaacgcagag
     2461 gccgatgtcg tctctcccgt cgaaggcacg acgatgctcg agctggcgaa cgagcctttc
     2521 tgggccgtcg gtctacgggc gggtggcaag tcgctcccgt ggtactcact gcccgggaac
     2581 cacgacatca agatccaggg gaccgccccg aacgaggccg gctggcgtga cttcgcaagt
     2641 cagtacgcgg tcggaggcgt gaagttcacc gacgttccac cggacaagca gcaagaagcc
     2701 tgtcaggcag ggttcagtga agagttcttc accgagatgg cgaccaaccc tggtgctgcc
     2761 gagccggtgc cggccgatcc cgaccgcagg cttctgtcgc gagaagagtg ggctcaggag
     2821 cacctcacga cgacaggcgt gcctgagggg cacggttacg gaggcgagcg ctgtaaggac
     2881 gacgccggcg aacttctgga gcgactctgc tactcctgga ccgaagggga cttccactac
     2941 atcggtcttg atacgaatcc ccacgagggt ctcgaggacg ggaacatcga cgacccgcaa
     3001 ttccagtggc tcgaaaagga cctacaggct cattcctcca cctactacac ggccgaaggt
     3061 ctgaggaaga agaacccgtc tggggacgac aagctgatcg tggttttcgc ccaccaccca
     3121 caacaatcga tgacgaacac atcgaccgag gggtctcatc aggctcaaga tctgaagcag
     3181 ttgctactcc gtttccctaa cgtcatcgtg atggcgaacg ggcacactca ccagaaccgc
     3241 atctgggggc gcatcaacaa gaaggccaag accggttact gggaggtgaa cacgtcggcg
     3301 atcgccgact tcccgtcgca gagcaggacc gtcgagatcg tgaagaacgg cgacggcacc
     3361 ctgtcgatct tcgcagtggt gttcgacgcc gccgtagcgc ccgacgtgcg atcgatggac
     3421 tgggccgacg atcccaccaa tgaggtcgat ctaggcggcg ccgagaccgc catcaacgag
     3481 gattggctcg cgtcttacgg acgcgaggtg atgttgtacg acccacaaca ggacctgatg
     3541 aagctcggta ctccaaagga tcgcaacgtc gagctgctgc tacgagcgcc tcgctggttc
     3601 cggtagttga gtgactcaga ccgcgagcac accaaagagg taaccgagca gttcacgcgg
     3661 gctgccgagg cgttctcgga gcgtagccgc ggccgtttcg acgtgctcga ccccgttgcg
     3721 ttttctcgtg tcgagccggg gcagagcgtg attgaggtgg gtggaggctc ggggaacttc
     3781 atctccaagt tccagtccat cgccggattg gcggcgatat tcgatctgac tccggcgatg
     3841 ctcggtgtag ctcgacgaac gtatccgggg ctggctctcg tggtcgccga tgcagagcgt
     3901 ctgccactcg catcgggctc ggcggacctg gcgaccaccg cgcaagctct tcatcacatg
     3961 gcgacccccg tcgaggtcgt ccgcgaactc agacgcgtga cgaaacggtc cggccgcgta
     4021 ctcgtcgtcg actcggtcgc tcccgatcgg atagaggaag cacgagccat gaacgagttg
     4081 gatctgatcc gggacccgag ccacgtcgcg ttccgttcgc cggcagctat gaaggtgatc
     4141 gttcaggcgg ccgggctcga tatcgtcgac gagcacctct cggtcgagca acagaagttg
     4201 tcgcagtgga tgtggcccga tgagtttcct ggcgaacgga tcgaggcggt gcgaaatttc
     4261 atcgaggaac ggggggccga gactggtatg aagttcgagc gttcgggagg cgactgggtc
     4321 ttcgagaggc gccgtttgat gctcctgtgc gaaagggcca agtgaaggaa agaagtggcg
     4381 gcaccgacta aagcaaaggc caagaagcgc gttgccgagt tgcgcgacgc gatcgactat
     4441 cactcctaca ggtaccacgc tctcgacgac cccgagatcg cagatgccga gtacgacgca
     4501 cttgtcggcg agctgatcga gctggagacc gagtaccccg acttgatcac tccggagtct
     4561 ccgacgcagc gggttggagc ccctccttcg gatctgttcg cggccgcgga gcacctgagt
     4621 ccgatgatgt cgctcgacaa ctgtttctcc ctcgaagaac tccaggcatg ggggaagcgt
     4681 gttgagcgag ggatcgggaa ggcggacgga ttcgtcgtcg aactgaagat ggatggggtc
     4741 gccgtcaacc tgatctacga gaagggggtc ctcgttcgtg ccgccacgcg aggcgacggt
     4801 cgggtgggcg aggacatcac ggcgaacgtc aagacgatcc gggcagtgcc gttgaagctc
     4861 cgcggcaagc ctcccgagat catcgaggtg cgcggtgaaa tctatatgcg caccgacgat
     4921 ttcgagaagc tgaactcgat gctcggggag caaggacata agacgttcgc aaaccctcgc
     4981 aatggggccg cgggctcact ccggcagaaa gatccgacgg tgactgccca gcggaacctg
     5041 tccctcgtct gtcacgggac cggccagatc caaggcctgc gattcccacg gcactggggc
     5101 gcgctcgaga cgatccgcga gctcggtctt cgcacgaatc ccgagaacaa gcaggtcgag
     5161 acgttggatg acgtgcacgc gttctgcaga cactgggagg agcagcgcca cgacgtcccg
     5221 tatgagatcg atggtgtcgt ggtcaaggtc gattcgatcg cgcagcaaga agagctgggc
     5281 tggacgtcga aggcaccgcg ctgggcgatc gcttacaagt tcccgcccga ggagcgcacc
     5341 acggttttga aaaggatcga agcctctgtg ggtcgaaccg gcgtcgtaac cccgttcgca
     5401 cagttggaaa cggtcttcgt gggtggcgtg aacatcacca ctgccacgct tcacaacgag
     5461 gatgaggtcg cccgcaaaga cgtgcgccag aaggacacgg tgatcgtgcg ccgagcgggg
     5521 gatgtcatcc cggaggtcgt cggtccggtg ttgtcgaagc gtccgaaggg aacgaagcgg
     5581 tggaagatgc cgcgcaagtg tccgtcgtgt ggctccgagt tggtgcgagc cgaaggcgag
     5641 tctgcaaccc gttgcctcaa tatctacgaa tgcagtgctc agagacgtga gcggatcttc
     5701 cacttcgcaa gccgcggcgg catggatatc gagggcttgg gagtccaaac catcgtggct
     5761 ttgatcgagc aggaatggct gaaggatgtc agcgacatct acttccttac gcgcgagcag
     5821 gtcctcgagc tggagggatt
//
LOCUS       DATMED010000464         4031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_4978309, whole genome shotgun sequence.
ACCESSION   DATMED010000464 DATMED010000000
VERSION     DATMED010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4978309"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="VNP73_12105"
     CDS             <1..1646
                     /locus_tag="VNP73_12105"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWL66710.1"
                     /translation="RRTFEQLRDAFGMGVAPLSLPLGREHDFRGVISVIDQAAFGYDE
                     SGKPTEEAVPEDRQARLTEVRTALLDSVAETSDELLEKYLEAGDLDRDQVLKALHDGI
                     AQATVFPVLVGAATHLIGIDRLADAIVSAGPSPLDRAPMEGEGGAREPKTDVPMSALV
                     FKTMTDPYVGRVSFLRVYSGIFKGDTSIYNATKKADERVAHPFTMRGKEQIPLSEIVA
                     GDIGAIAKLHETVTGDTIADKSNPVVFEVIEPPEPLLPKAIEPKTKGDEDKLMIGLHK
                     LIEEDPALRLDRNDETHQTILWGMGDAHLDVTLERLKRKFSVEVGDVPFKVAYRTTLR
                     GKADALGRHVKQSGGHGQYGVCNVRVEPLPRGEGFVFDNKIFGGSIPSQWVPSVEKGV
                     RAAMEDGVGTGFKMIDIKVELYDGKFHSVDSSDMAFQLAGTHAMKDAIDKAGVNVLEP
                     IWSISVMVPEEFTGDIMGDLQKRRGIPEGIDTIGGGRQIVKAKVPFGETTHYATDLRS
                     MTGGKGSFSWEFSHYQEVPHDVAEKILAAARAEKEEAKT"
     gene            complement(1684..1824)
                     /locus_tag="VNP73_12110"
     CDS             complement(1684..1824)
                     /locus_tag="VNP73_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66711.1"
                     /translation="MQKVWVDCTAPEPYLGVTLAAMVAAGVVWILVEKRLRRPADAEL
                     VA"
     gene            complement(2027..2518)
                     /locus_tag="VNP73_12115"
     CDS             complement(2027..2518)
                     /locus_tag="VNP73_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWL66712.1"
                     /translation="MIFERLWTPWRMEYMDKVGGSGEGCFLCDAFESNDDVARFVVFR
                     GATGFVIMNAYPYNTGHVMVAPNRHVAGLSELDAAERGELMEVTNHTIEVISEVLNPE
                     GFNTGMNLGRVGGAGVPGHLHMHVVPRWVGDTNFMPVIGETKVLPELLEETAAKLRTA
                     FLG"
     gene            complement(2515..3159)
                     /locus_tag="VNP73_12120"
     CDS             complement(2515..3159)
                     /locus_tag="VNP73_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWL66713.1"
                     /translation="MSERLVEEQIAYYDQRAAEYDATSMTCDDPQYEYVEQIEKALAD
                     FRPTGKVLEFACGTGNWTSRLQPFADEVLALDSSPQMLEFARTKVSAGNVRFEQADLF
                     AWEPPDAYDVVFFSFWISHVPQERFDEFWELVHCCLTRKGRIFFIDERPHEHWHEEYI
                     DEQLVQRTLQDGSRHRVVKKFWAEGELEQRLRGLGWSVSVTGTGPFFWAEGSRA"
     gene            complement(3159..>4031)
                     /locus_tag="VNP73_12125"
     CDS             complement(3159..>4031)
                     /locus_tag="VNP73_12125"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWL66714.1"
                     /translation="YRDLPIRLAELGTVYRYERSGVLHGLLRGRGFTQDDSHIFCRAD
                     QMVEEMVDCVRFFQDLYAHVGMGADEVHFSTRPDKSVGTDEQWKLAEEAIVKAIEESG
                     LDFKVAEGEGTFYGPKIDIHVRDAIGRLWQVATIQVDFQNPQRFELEYVDEHGARVEP
                     VMIHRALYGSIERFTGVITEHFGGAFPTWLAPVQAVLIPIADRHVAYASAVAERLSAK
                     RVRVEVDDSDNTMGAKIRHQQLQKVPYMLIVGDDEAEKESVSVRPRSGDERRGVAVGD
                     FLDEITREIEERRL"
BASE COUNT          811 a         1309 c         1179 g          732 t
ORIGIN      
        1 tccgtcggac cttcgagcaa ctccgcgacg cgttcggtat gggagtcgct cccctgtcgc
       61 tgccactcgg acgcgagcac gacttccgtg gcgtcatctc cgtcatcgat caggccgcgt
      121 tcggttacga cgagtcgggt aagccgaccg aggaagccgt tcccgaagac cggcaggcgc
      181 gactcaccga ggttcgcact gcactgctcg actcggtcgc ggagacatcc gacgagttgc
      241 tcgagaaata cctcgaagcc ggcgacctcg atcgcgacca ggtactgaag gcgctccacg
      301 acgggatcgc gcaagcgacc gtcttcccgg tgctcgtcgg tgcggcaaca cacttgatcg
      361 gcatcgatcg gctcgccgat gcgatcgtct cggccggccc gtctcctctg gatcgtgcac
      421 cgatggaggg cgagggtgga gcacgcgagc ccaagaccga cgtcccgatg tccgcactcg
      481 tcttcaaaac gatgaccgac ccctacgtcg gtcgggtctc gttcctgcgc gtctactccg
      541 gcatcttcaa gggcgacact tctatctaca acgcaacgaa aaaagccgac gagcgcgtcg
      601 cacatccgtt cacgatgcgc ggcaaggagc agatccctct atccgagatc gtcgccggcg
      661 acatcggtgc gatcgcgaag ctgcacgaga ccgtgaccgg tgacacgatc gccgacaagt
      721 cgaacccggt cgtattcgaa gtcatcgagc ctcccgagcc gctcttgccc aaagcgatcg
      781 agccgaagac gaagggtgac gaggacaagc tgatgatcgg cttgcacaag ttgatcgaag
      841 aagatccggc attgcgactg gaccgcaacg acgagactca tcagacgatc ctgtggggca
      901 tgggtgacgc tcatctcgac gtgacgctcg agcgtctcaa gaggaagttc tcggtcgagg
      961 tcggagacgt tcccttcaag gtcgcctacc ggacgacgtt gcgaggcaaa gccgacgctc
     1021 tcggccgcca cgtgaagcaa tccgggggcc acggccagta cggggtgtgc aacgtccggg
     1081 tcgagccact gccccgcgga gagggcttcg tcttcgacaa caagatcttc ggaggctcga
     1141 tcccctcgca gtgggtgccg tcggtcgaga agggtgtgcg ggcagcgatg gaagacggag
     1201 tcggcaccgg tttcaagatg atcgacatca aggtcgagct ctacgacggc aagttccaca
     1261 gcgtcgactc gtcggatatg gcgttccagc tggccggaac gcatgcgatg aaggatgcga
     1321 tcgacaaagc cggcgtcaac gtcctcgagc cgatctggag catctcggtg atggtccccg
     1381 aggagttcac cggcgacatc atgggtgacc tacagaagag gcgaggcatc cccgagggca
     1441 tcgacacgat cggcggcgga cgccagatcg tgaaggccaa ggtcccgttc ggagagacga
     1501 cgcactacgc gaccgacctt cgctcgatga ccgggggcaa aggcagcttc tcgtgggagt
     1561 tcagccacta ccaagaggtt ccgcacgacg ttgcggagaa gatccttgcg gcggcgaggg
     1621 ccgagaagga agaagcgaag acctagccct tcttggctaa ctgagtaacc ttctagcgcg
     1681 ctctcacgcg acaagttcag catctgctgg acgccggaga cgcttctcga ccaggatcca
     1741 aacgacacca gcggcaacca tcgcggccag ggtgactccg aggtaaggct cgggcgctgt
     1801 gcagtcgacc cagactttct gcatgaggcc attaggcagc cttctcactg gcggttgcgg
     1861 gcaccatccc cttcttagaa cccccgcgtc gatcaagaag acctgaccta gtgtcgccgc
     1921 gactcccact gcgagcaaac taacgagagc caccggcagc cggatccatc tgttcttcat
     1981 cactcaccca acttgtcgcg tcagtgcgcg ctagaaggtt actcagttag ccaagaaagg
     2041 cggtgcggag tttggcggcg gtttcttcta ggagctcggg caggactttc gtttcgccta
     2101 tcaccggcat gaagttggtg tctcccaccc agcgcggcac cacgtgcatg tgaaggtgac
     2161 ccgggacacc agccccccca accctgccaa ggttcatccc cgtgttgaac ccctccggat
     2221 tcaacacctc ggagatcacc tcgatcgtgt ggttcgtcac ctccatcagc tcgccgcgct
     2281 ccgcagcgtc gagttcggac agtcccgcca cgtgccggtt cggcgcgacc atcacgtgcc
     2341 ccgtgttgta cggataagcg ttcatgatca cgaagccggt cgccccccgg aacacgacaa
     2401 acctcgctac gtcgtcgttg ctttcgaacg catcgcatag gaagcagccc tctcccgagc
     2461 ctccgacctt gtccatgtac tccatcctcc acggcgtcca cagacgctcg aagatcacgc
     2521 tcgcgagcct tcggcccaga agaacgggcc ggtcccagta accgacaccg accatccgag
     2581 gccgcgcaac cgctgctcga gctcaccttc ggcccagaac ttcttcacca cgcgatggcg
     2641 gctaccgtct tgcagcgtcc tctgaacgag ctgctcatcg atgtattcct cgtgccagtg
     2701 ctcgtgcgga cgctcgtcga tgaagaagat ccttcccttg cgggtcaagc agcaatgcac
     2761 gagctcccag aactcgtcga aacgctcctg gggcacgtgc gagatccaga acgagaagaa
     2821 caccacgtcg taggcatcgg gtggttccca cgcgaagaga tccgcttgct cgaagcgaac
     2881 gttgcctgcg gacacctttg ttcgtgcgaa ctcgagcatc tgcggcgacg aatcgagggc
     2941 caggacctcg tccgcgaatg gttgcaatcg cgacgtccag ttcccagtcc cgcacgcgaa
     3001 ctccagcacc tttccggtgg gccggaaatc ggccagtgcc ttctcgatct gctcgacgta
     3061 ttcgtactga gggtcgtcac aggtcatcga ggtcgcgtcg tattcggccg cccgttggtc
     3121 gtagtaagcg atctgctcct cgacgagccg ctcggacact agaggcgtcg ctcttcgatc
     3181 tctctggtta tctcgtccaa gaagtcaccg acagcaacgc cgcgccgttc gtcgcccgac
     3241 cgcgggcgga cggacaccga ttctttctcg gcctcgtcgt ccccgacgat cagcatgtag
     3301 ggcaccttct ggagttgctg gtggcggatc ttggccccca tcgtgttgtc ggagtcgtcg
     3361 acctcgacac ggaccctctt cgccgacagc cgctcggcga ccgccgacgc ataagccacg
     3421 tgccgatccg cgatcgggat gagcaccgcc tgtacgggag cgagccaggt tgggaaagcg
     3481 ccgccgaagt gctcggtgat aaccccagtg aaacgctcta tcgagccata cagagcgcga
     3541 tggatcatca cgggctcgac ccgcgccccg tgctcgtcga catactccag ttcaaatcgt
     3601 tgaggattct ggaagtcgac ctggatcgtg gctacctgcc acaggcggcc gatcgcatcg
     3661 cgcacgtgga tgtcgatctt gggtccatag aaggtgccct caccctcggc gaccttgaag
     3721 tcgaggccac tctcctcgat cgctttgacg atcgcttcct cggccagttt ccactgctcg
     3781 tcggtaccaa ccgatttatc cggcctcgta gagaaatgaa cctcgtcggc gcccatcccc
     3841 acatgggcgt atagatcctg gaagaaccgg acgcagtcca ccatctcttc caccatctga
     3901 tcggcacgac agaagatatg actgtcgtcc tgagtaaagc cgcggccgcg caacagaccg
     3961 tgcaagacgc cggaacgctc gtatctatag acggtcccca gctccgcaag acggataggg
     4021 agatcacgat a
//
LOCUS       DATMED010000465         3803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1916926, whole genome shotgun sequence.
ACCESSION   DATMED010000465 DATMED010000000
VERSION     DATMED010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916926"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="pgk"
                     /locus_tag="VNP73_12130"
     CDS             complement(<1..838)
                     /gene="pgk"
                     /locus_tag="VNP73_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWL66715.1"
                     /translation="MAELPSLDELDLRDARVLTRCDLNVPLSDGSITDDMRIRAAIPT
                     LEAILERGGRLAVCSHLGRPKGTRVEGLSLAPVAQRLAGLLDRQVEFATDAAGEDSQR
                     LCASDAAVVLLENLRFEPGEASNDPGFADRLAALGTAYVNDAFGAAHRAHASIVGVPE
                     RLPGAAGLLLQDEVAKLTRLLDEPESPFVAVLGGAKVSDKLAVIGNLLDRVDAICVGG
                     AMAFTLLVARGEEVGRSLVERDQVDTVTDVLAEAKRAGIDILLPVDVVAATAPEEGAE
                     HAE"
     gene            925..1077
                     /locus_tag="VNP73_12135"
     CDS             925..1077
                     /locus_tag="VNP73_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66716.1"
                     /translation="MNDKHPHCPDCDSKDISITPVPGLWVQYWCNDCDHFWWERSDVA
                     PETSPG"
     gene            complement(1074..2081)
                     /gene="gap"
                     /locus_tag="VNP73_12140"
     CDS             complement(1074..2081)
                     /gene="gap"
                     /locus_tag="VNP73_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020120076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWL66717.1"
                     /translation="MTTRIGINGFGRIGRNFYRALRKRDSGLELVAVNDLGDAATMAH
                     LLRYDSVLGRFEEEVKVTDAGFTVGGADIKLLSERDPGALPWGDLGVDVVIESTGFFT
                     KRADASKHLDGGAKKVIISAPAKDEDITIVMGVNDGEYDPSGHNVISNASCTTNCVVP
                     LAKVLQDNWGIESGFMTTCHAYTNDQNTLDLAGGDLRRSRAAAINIIPTTTGAAKAAA
                     LAMPELKGRLDGLALRVPVPDGSITDIVALLGSSATKDEINTAYREASQSAGMKGILE
                     YTEDPIVSSDIVGNPHSCIIDGLSTMANGNLVKVLGWYDNEWGYSNRLVDLTALVASK
                     L"
     gene            complement(2125..3081)
                     /gene="yvcK"
                     /locus_tag="VNP73_12145"
     CDS             complement(2125..3081)
                     /gene="yvcK"
                     /locus_tag="VNP73_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HWL66718.1"
                     /translation="MSAQPERSERPERAAASAGALRVVGIGGGHGLATTLRATRLYAS
                     AIAAVVTVADDGGSSGRLTRDLGIPPPGDIRNCLAALAQDEALARLYQHRFESGALTG
                     HTVGNLVIAALTETEGSFGAAVRRAGEFLATVGDVFPATTELVRLAARVDGGEVHGQV
                     AVARTTEPIQAVYLDPPAPEAYPAAVDAILNADQVVLGPGSLFTSLIATLLVPGIRAA
                     LRSTRAVRVFVCNSRMQKGETEGLSASDHIGAVLAHVGPDCVDAAILQSPPVPTDGVE
                     LDEGGLDFLGIDLIRADLVERDGPHDPVRLAEVLKDVTSRMG"
     gene            complement(3078..>3803)
                     /gene="rapZ"
                     /locus_tag="VNP73_12150"
     CDS             complement(3078..>3803)
                     /gene="rapZ"
                     /locus_tag="VNP73_12150"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWL66719.1"
                     /translation="NELNRIALVIDARSGVFFEEATSALENVRRDVSNYRMVFLEASD
                     DALVRRFDASRRLHPLATDDNLQVGISRERELMSAFRDGADLILDTSDLSVRELRAKI
                     GAYFEGGTPGEGLKTTIISFGYKFGLPLDADIVLDVRFLPNPHWIEELRPHTGLDEPV
                     RDYVLGHDQTTEFLARSRDLFAVLLPGYQSEGRHYLTVGIGCTGGRHRSVVLAQELCA
                     LIEERGFRVKTIHRDVERPPVVP"
BASE COUNT          673 a         1274 c         1167 g          689 t
ORIGIN      
        1 cttctgcatg ttccgcgcct tcttccggag cggtcgcagc gacgacatcg accggcagca
       61 agatgtcgat ccccgccctc ttcgcctcgg cgaggacatc cgtgaccgtg tcgacctggt
      121 cccgctcgac cagcgacctc cctacttcct caccacgagc cacgagaagc gtgaacgcca
      181 tcgctccgcc gacacagatc gcatccacgc ggtcgagcag attgcctatg actgcaagct
      241 tgtcggagac cttggctccg cccaacaccg ccacgaacgg tgactcgggc tcgtcgagga
      301 gtctggtcag cttggctacc tcgtcctgca ataagagtcc ggccgctcct gggagacgct
      361 cgggaacccc aactatcgat gcgtgagcgc gatgcgctgc gccgaaggca tcgttcacgt
      421 acgcggtgcc cagagcggcc agccggtcgg cgaaaccagg atcgttgctg gcctcgccgg
      481 gttcgaacct gaggttctcg agcaggacga cggcagcatc ggaggcacaa agcctctggg
      541 aatcctctcc cgccgcatcg gtcgcgaact cgacctgtcg atcgagcaac ccggcaagac
      601 gctgggccac cggggcaagg ctcaatccct ctactcgcgt gcccttgggc cggcccagat
      661 ggctgcacac ggcgaggcga ccacctcgtt ccaggatcgc ctcgagggtc gggatcgcgg
      721 cccggatcct catgtcgtcg gtgatcgagc cgtccgacag agggacgttc aggtcacatc
      781 gggtcaaaac ccgggcatcc cggaggtcca gctcgtccag agatgggagc tctgccatgc
      841 ctgtagccgt tttccttcct cgagcgtcta agacccgtcc aggttaaacg tcgctctcca
      901 gaagcgactc cgagagaggc cgctatgaac gacaagcacc cccactgtcc ggactgcgac
      961 tcgaaggaca tctcgatcac gccggtcccc ggcctgtggg tccagtactg gtgcaacgac
     1021 tgcgaccact tctggtggga gcgttcggat gtcgctccgg agacgagccc cggttagagc
     1081 ttcgacgcga cgagtgcggt cagatccacc aaccggttcg agtagcccca ttcgttgtcg
     1141 taccaaccga gcaccttgac gaggttgccg ttcgccatag tcgagagccc gtcgatgatg
     1201 cacgagtggg ggttgcccac gatgtccgac gacacgatcg ggtcctcggt gtactccagg
     1261 atgcccttca tgccggccga ctgtgatgcc tcccgatagg cggtgttgat ctcgtccttc
     1321 gtcgccgacg aaccgagcag cgccacgatg tccgtgatcg aaccgtcggg gaccggaacc
     1381 cgcagggcca gtccatcgag cctgcccttt agctcgggca tcgcgagcgc cgcggcctta
     1441 gcggccccag tcgttgtcgg gatgatgttg atcgcggccg cacgcgaccg gcgcagatcg
     1501 ccgccggcga gatcgagcgt gttctggtcg ttggtgtacg cgtgacaggt cgtcatgaac
     1561 ccggactcga tcccccagtt gtcctgaaga accttggcga ggggtacgac acagttcgtc
     1621 gtgcacgacg cgttggagat cacgttgtgg cccgatgggt cgtactcccc atcattgaca
     1681 cccataacga tcgtgatgtc ctcgtcctta gctggcgccg agatgatgac tttcttcgcc
     1741 ccaccgtcga gatgcttcga ggcatcggca cgtttggtga agaaccccgt cgattcgatc
     1801 acgacgtcga cgccgagatc gccccacggg agtgctccgg gatcgcgctc tgagagaagt
     1861 ttgatgtccg ctccaccgac cgtgaagccg gcgtcagtaa ccttgacttc ttcttcgaag
     1921 cgtcccagta cggagtcgta gcgcagaagg tgcgccatgg tggcggcatc cccgaggtcg
     1981 ttgacggcga ccagctcgag gccggagtcc cgcttcctga gcgccctgta gaagttccga
     2041 ccgatacggc cgaagccgtt gatcccgatg cgtgtggtca tcagatcccc ttttcctgct
     2101 gtcttgttcc tgcttgcttg actgttaccc catcctactt gtgacgtcct tcagaacctc
     2161 cgccagtcgc acggggtcgt ggggcccgtc gcgctcgacc agatccgccc ggatcagatc
     2221 gatacccagg aaatcgaggc ccccctcgtc gagttcgaca ccgtcggtgg ggaccggcgg
     2281 cgactgcagg atcgctgcgt cgacacaatc cggaccgacg tgagcgagaa ccgcaccgat
     2341 gtggtcggaa gcactcagac cctcggtctc acccttctgc atccgactat tgcagacgaa
     2401 caccctcacg gcgcgcgtgc tgcgaagtgc ggctctgatg ccgggcacca gcagagttgc
     2461 tatcaggctc gtgaacaagc ttccggggcc gagcacgacc tggtctgcgt tcaggatcgc
     2521 atcgacggcg gccgggtaag cctcgggggc cggcggatcc aagtagacgg cctggatcgg
     2581 ctcggtcgta cgcgcgacgg cgacctgccc gtgcacctct cccccatcga cgcgagcggc
     2641 gagtcgaacg agctccgtcg tcgcggggaa tacgtcaccc accgtcgcaa ggaactcgcc
     2701 ggcgcgccgg accgctgcgc cgaagctccc ttccgtttca gtcagagctg cgatcacgag
     2761 attgccgacc gtgtgcccgg tgagtgcgcc gctctcgaac cgatgctgat agagacgtgc
     2821 cagcgcctca tcctgcgcca gcgccgcaag gcagttgcgt atgtcacccg gaggcgggat
     2881 accaagatcg cgcgtcagcc gtcccgacga tccgccatca tctgcgaccg tcacgaccgc
     2941 ggcgatagcg ctcgcgtaca gccgggtcgc gcgcagagtc gtagccagcc cgtggcctcc
     3001 cccgatccca accactctta gcgcgcccgc cgaggcggcc gcgcgttccg ggcgttccga
     3061 gcgttccggc tgcgcgctca tggcaccacc ggcggtcgct cgacgtctcg gtggatcgtc
     3121 ttcactcgga agccccgctc ctcgatcagg gcacacagtt cctgagccag caccacggag
     3181 cgatgtcggc cgcccgtgca tccgatcccg accgtcaggt aatgcctgcc ttccgattgg
     3241 tagccgggaa ggagcacggc gaacagatct cgagatcgtg ccaggaactc ggtcgtctga
     3301 tcgtgcccga gcacgtaatc gcgtactggt tcgtccagtc cggtatgcgg tcgcagctcc
     3361 tcgatccagt ggggattcgg aaggaagcgc acgtccagaa cgatgtcggc atcgagtgga
     3421 agcccgaact tgtagccgaa cgagatgatc gtcgtcttca agccctcgcc gggggtaccg
     3481 ccctcgaagt aggccccgat cttggcgcgg agctctcgca cagacagatc agatgtgtcg
     3541 aggatcaggt cggcgccgtc ccggaaggcc gacatcaatt cccgctcgcg tgagatcccc
     3601 acctgcaggt tgtcatcggt agcaagagga tggagacgcc tgctcgcgtc gaaccgacgc
     3661 acgagggcgt cgtccgaggc ttcgaggaag accatcctgt agttcgacac gtcccgtcgc
     3721 acgttctcta gggccgaggt cgcctcttcg aagaacactc cgctgcgcgc atcgatgacg
     3781 agcgcgatgc gattcagctc gtt
//
LOCUS       DATMED010000466         3313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3826940, whole genome shotgun sequence.
ACCESSION   DATMED010000466 DATMED010000000
VERSION     DATMED010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826940"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VNP73_12155"
     CDS             <1..443
                     /locus_tag="VNP73_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWL66720.1"
                     /translation="NAYFNLTKLGASITAYFPSFYGPMNDGINGRDDSDRVLVVWALD
                     SQKATEASTGNLKAPDLEMLTRSGAEVALKVDTNDRPVEMDASGDTILVGVPRDIVEI
                     RRNDPDLAHLWRLATRDILSGALNDGYEVVAMTRSGYYVLERIK"
     gene            complement(436..1557)
                     /gene="menC"
                     /locus_tag="VNP73_12160"
     CDS             complement(436..1557)
                     /gene="menC"
                     /locus_tag="VNP73_12160"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HWL66721.1"
                     /translation="MLLEAIELRRIALRMKTPFRTSFGIQQDRDVLLVKTITSSGEGW
                     GECVAMSAPHYSSEYIDGAQEVIKRFLAPPLFEPAAITAAHVEPLLAGIKGHRMAKAA
                     VEMAILDAELRSLGQSFAAALGGVQDMVDTGVSVGIHDDIGELLDTVDGYVDQGYIRI
                     KLKIEPGNDIEHVRAVREKFGPDLPLQVDANTAYRLSDARHLARLDEYGLLLIEQPLP
                     EEEVRAHAELARQVKTPICLDESITSVQAAADAIAIGACSIVNIKAGRVGGYLEAKRI
                     HDLCLGHGIPVWCGGMLETGIGRAANVALASLPNFTLPGDTSASDRYWERDITEPFVL
                     ERGRVRVPTGPGLGVEPVPEFLEEVTTSVETITRTTAAT"
     gene            complement(1547..2557)
                     /locus_tag="VNP73_12165"
     CDS             complement(1547..2557)
                     /locus_tag="VNP73_12165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HWL66722.1"
                     /translation="MKSPVVDCHNDWILMFARERSLEHTDSLERRFLPQFKAAGIDVQ
                     VTPIYIDPEFIPEGALRRTLLLIQYLKEEVASCDEVALCNTGAEIDAAVSDGKLALVL
                     ALEGSHAIGTDVQLFQALFDLGVRMASFTHFGNTSLAGGSADGDPGMGLSSHGIEALR
                     SFERLGLVMDVSHLSTQATEEVLQHATRPLVASHSSLKALRDHHRNLSDEHAKGIAAL
                     NGVIGVPAAIPIFIDPEYPTIDRVVDHIEHLAETVGIDHVGIGADYVREYFDEVYATY
                     EKIEHQGEDVRAVIQGLDAPAGMPNLIEALERRGFEGDDLSKVLGGNFLRVFREVMGI
                     AA"
     gene            2705..>3313
                     /locus_tag="VNP73_12170"
     CDS             2705..>3313
                     /locus_tag="VNP73_12170"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWL66723.1"
                     /translation="MTVGEQELQNRVKELSEELAVPGVAVGVLIDGQEHYAFHGVTGI
                     ESPLEVDANTLFQFGSTGKTFTATAIMRLVERGDVDLDAKVRKYVPDLKLKDESVAEN
                     VTILHLLNHTAGWSGDLMENTGDGDDAIAKYVAKMVDIEQVTPLGATASYNNAALSLA
                     GLVIEKVTGQTYEQAMKELIFEPLGLSNMYFFPSEIMTRRFAI"
BASE COUNT          638 a         1033 c         1025 g          617 t
ORIGIN      
        1 gcaacgccta tttcaatctg accaagttgg gggcgtcgat cacggcttac ttcccgagct
       61 tctacggacc gatgaacgac gggatcaatg gccgcgacga cagcgaccgg gttctcgtgg
      121 tgtgggcgct cgactcccag aaggctacgg aggcaagcac cggcaacctg aaggcaccgg
      181 atctcgagat gctcacgcgt tcgggggccg aggtcgctct gaaggtcgac accaacgatc
      241 gtccggtcga gatggacgcg tcgggcgaca cgatcctcgt cggcgtgccc cgggacatcg
      301 tcgagatacg ccgcaacgat ccggacctgg cacacctgtg gcgactcgcg acccgcgaca
      361 tcctttccgg agccctgaac gatggttacg aggtcgttgc gatgacgcgt tccggctact
      421 acgtgctcga gaggatcaag tagccgcagt tgtccgcgtg attgtttcga ccgaagtcgt
      481 gacttcctcg aggaactcgg gcaccggttc gacacccaag ccgggccctg tgggcacccg
      541 gacccggcca cgttcaagca cgaacggttc cgtgatgtca cgttcccagt agcgatccga
      601 cgccgaggtg tcacccggga gggtgaagtt gggcagcgac gcaagtgcga cgttcgcagc
      661 acggccgatc ccggtttcga gcatcccccc gcaccacacc gggatcccgt gtcccaagca
      721 gaggtcgtgg atccgcttcg cctccagata accgccgacg cgacctgcct tgatgttcac
      781 gatcgagcac gccccgatgg cgatcgcgtc ggccgccgcc tggaccgacg tgatcgactc
      841 gtccaggcag atcggtgtct tgacctggcg tgccagctcg gcatgggccc gcacctcttc
      901 ctcgggcagt ggctgttcga taagcagcaa gccgtattcg tcgagccgcg cgagatggcg
      961 cgcatcggac aaccggtaag cggtgttggc atcgacctgt agcggcaggt cgggcccgaa
     1021 tttctcccgc accgcccgca cgtgctcgat gtcgttaccc ggctcgatct tgagcttgat
     1081 ccggatgtag ccctggtcca cgtaaccgtc gacggtgtcg agcagttcgc cgatgtcatc
     1141 gtggatccca acggagacac ccgtgtcaac catgtcctgc acgccaccga gcgcggcggc
     1201 gaaggactga ccgagcgagc gtagttccgc atcaaggatc gccatctcga cggcggcctt
     1261 tgccatccgg tgacctttga tcccggccag gagcggttcc acgtgcgcgg ccgtgatcgc
     1321 tgcgggttcg aagagtgggg gggccaggaa tcgtttgatc acttcttgag cgccgtcgat
     1381 gtactcgctt gagtaatgcg gcgcggacat ggcaacgcat tccccccagc cctcgccgga
     1441 cgatgtgatc gttttgacga gcaggacgtc gcggtcctgt tggatcccga acgaggtcct
     1501 aaacggcgtc ttcatgcgca gcgcgatcct gcggagctcg atcgcttcaa gcagcaatgc
     1561 ccatcacctc tcggaagact cgtaagaagt ttccacccag cactttgctc aggtcatcgc
     1621 cttcgaaacc gcgtcgctct agggcttcga tcagattggg cattccggcc ggagcgtcga
     1681 gcccttggat caccgcccga acgtcctcgc cctgatgttc gatcttctcg taagtcgcat
     1741 agacctcatc gaagtactca cggacgtagt cggcgccgat gcccacgtgg tcgatcccga
     1801 ccgtctccgc taggtgctcg atgtggtcga cgacccgatc gatcgttggg tactcggggt
     1861 cgatgaagat cgggatcgcg gcggggaccc cgataacgcc gttgagcgcg gcgatccctt
     1921 tcgcatgctc gtcgctgagg ttgcggtgat gatcgcgcag ggccttcagc gatgaatgcg
     1981 acgcaacgag tggcctggtg gcgtgctgca atacttcctc ggtcgcctgg gtcgaaaggt
     2041 gcgagacgtc catcacgaga ccgagacgtt cgaacgatcg cagagcttcg atcccgtgcg
     2101 acgacaaccc catccccggg tcgccatccg cgcttccacc ggccagagac gtgttcccga
     2161 agtgcgtgaa cgaagccatc ctcacgccga ggtcgaacaa tgcttggaat agctgaacgt
     2221 cggtgccgat cgcatggctt ccttcgagcg caagtacgag ggccagcttc ccgtcactta
     2281 ccgcggcgtc gatctcggcc cccgtgttgc acagtgcgac ttcatcgcac gaagccactt
     2341 cctccttcag gtactggatc agaagcagtg tgcggcgaag ggctccttcg gggatgaact
     2401 ccggatcgat gtagatcggg gtcacctgaa cgtcgatgcc ggcggccttg aactggggga
     2461 ggaacctgcg ctcgagactg tcggtgtgtt ccagtgagcg ctcgcgcgcg aacatcagta
     2521 tccagtcgtt gtggcaatcg acgacggggc tcttcatcgt tagcagtttc gcaccgaggc
     2581 aagggagggg tgttcggacc caaccacaat cgacagcagt cggattggtt cgcacccaca
     2641 gcggttctgc gtcctcgccc ccttaccttg ctgaaaacgg atcttcagtc aaagggagtg
     2701 gcgcatgacc gtcggcgaac aggaactcca gaatcgggtc aaggagctaa gtgaagagct
     2761 cgccgtaccg ggggtcgccg taggggtctt gatcgacggg caagagcact acgcgttcca
     2821 cggagtcacg ggcatcgaga gcccgctaga ggtggacgcc aacaccctgt tccagttcgg
     2881 ctcgacggga aagacgttca ccgcaacggc gatcatgagg ctggtcgagc gcggagatgt
     2941 ggatctcgac gcaaaggtgc ggaagtacgt gcccgacctg aaactcaaag atgaaagtgt
     3001 tgccgagaac gtaacgatcc ttcatctcct caaccacacc gccgggtggt cgggcgacct
     3061 catggagaac acgggcgatg gcgacgacgc gatcgccaag tacgtcgcca agatggtcga
     3121 catcgaacag gtcacgcccc tgggtgccac ggcctcgtac aacaacgcgg cgctatcact
     3181 cgccgggctt gtgatcgaga aggtgacggg ccagacctac gagcaggcaa tgaaggagct
     3241 catcttcgaa cccctggggc tcagcaacat gtacttcttt cccagcgaga tcatgacccg
     3301 ccgcttcgcc atc
//
LOCUS       DATMED010000467         4919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_3828513, whole genome shotgun sequence.
ACCESSION   DATMED010000467 DATMED010000000
VERSION     DATMED010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3828513"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VNP73_12175"
     CDS             complement(<1..289)
                     /locus_tag="VNP73_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase II"
                     /protein_id="HWL66724.1"
                     /translation="MRVEAGIHRDLGLTDEEFDKIRELLDRDPTYTELAMFSVMWSEH
                     CSYKSSKVHLKTLPTEGEHILVGPGEGAGIIEVAPGVAVAWKIESHNHPSFI"
     gene            complement(286..1002)
                     /gene="purQ"
                     /locus_tag="VNP73_12180"
     CDS             complement(286..1002)
                     /gene="purQ"
                     /locus_tag="VNP73_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWL66725.1"
                     /translation="MNAKAAVVVFPGSNCEQDVERALRSLGCQTQRVWHAAETLPDTD
                     LVVLPGGFAHGDYLRTGAIARFSPVMAAVQEHAATGGLVIGICNGFQILCEAGMLPGA
                     LRKNAGLKFLCRWTDLRVENNATPFTSRAEVGQVVRVPINHFEGNWYCDDDTLADLRD
                     SGSILLRYVDNPNGALDDVAGITNKDRTVFGLMPHPERACEALLGSEDGRVIFESMID
                     HALQAGSPDPAGRVPEMLGT"
     gene            complement(999..1226)
                     /gene="purS"
                     /locus_tag="VNP73_12185"
     CDS             complement(999..1226)
                     /gene="purS"
                     /locus_tag="VNP73_12185"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWL66726.1"
                     /translation="MLKSGIADPQGQTIERALPALGYTGVTGVRVGKIIELHVDAADE
                     TEARRRVDEMCERLLANPVIESYEVTIGDPG"
     gene            complement(1232..1990)
                     /locus_tag="VNP73_12190"
     CDS             complement(1232..1990)
                     /locus_tag="VNP73_12190"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWL66727.1"
                     /translation="MSDSVADPARPMSRADRSRASARLGLTFRTVLLAPREGFEAAVK
                     ATERRQRSGRWPIEGLSTYVLTALGGASLAALWLKMGALFKLRQVCTAEYIPAYVALA
                     LVVGAVLALIAQFAWGAFGRSVIESLKGSAGSSGLRLVWGASALPQILVLVVLIPLDL
                     LIVGTPTFTTQPLNESLSTAWAAFSIALSVSLAVWSIYLFVRGVEVVGGLPARRAVVA
                     TAFAALCLAVAMAALVVPTALTVSKEAACPTRLG"
     gene            complement(1987..2868)
                     /locus_tag="VNP73_12195"
     CDS             complement(1987..2868)
                     /locus_tag="VNP73_12195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWL66728.1"
                     /translation="MSSQAKHVGSGKVRELFEIGEDSLLLVATDRISAYDVVLTQDIP
                     DKGKVLTGLSNYWFEVMDEIPNHLISIRAEDLPDIGIDDLAGRAMLCRRAEPVAIEFV
                     VRGYLSGSGWKEYKANRSVCGHQLPEGLKESDQLPEVLLTPATKATTGHDENITEGEA
                     AEIAGKDVYDTARTYALEVYKKAAALALERGIIIADTKFEFGVVDGEVILIDEVLTPD
                     SSRFWPEDKYEPGRGQPSFDKQYVRDWLDSTGWDHSPPPPDLPEEVVAATRDRYVEAY
                     ELVSGRPFEAWLKEVSA"
     gene            complement(2865..4160)
                     /gene="purB"
                     /locus_tag="VNP73_12200"
     CDS             complement(2865..4160)
                     /gene="purB"
                     /locus_tag="VNP73_12200"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWL66729.1"
                     /translation="MIPRYSREEMAAIFSEQAKFSRWMEIEILAVEARVRLEQVPVED
                     LTEIKEKAAFDVARIAEIEEVRQHDVVAFVENLRENVGEAGRHIHYGLTSSDVLDTAT
                     AAALRDAGDLLIEGVGRLTETIRRKALEHKDTVMAGRTHGVHAEPTTFGLKLAGWAFE
                     LSRDRDRLRAARDRVAVGKLSGAVGSYSQLPPDVEAFVCDRLGLGIEDAATQVTARDR
                     HAEFLAAIAITGASLERFAQEIRHLQRTEVREVEEPFGKGQKGSSAMPHKKNPITCER
                     ICGLARLLRAYAATGFENVALWHERDISHSSVERITLGDACILLDYMMDKLRWVVDEL
                     RVYPDRMLENLDASWGLVHSQSVLTALLAKGLQRESAYELVQRNAMAAWDEGRHLKEL
                     LKSDADVTQHLDPDEIERCFDPTRYLEYADEIFRRLEAL"
     gene            4248..>4919
                     /locus_tag="VNP73_12205"
     CDS             4248..>4919
                     /locus_tag="VNP73_12205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWL66730.1"
                     /translation="MSSGGSLSFDRAAEYYDATRGRSDEIERSVARLAEILGGRDRCL
                     EVGVGTGRVALPLHQKGIDLVGIDISARMMAKMREKTSGRHPFPLVQGDATRIPFRAH
                     SFDAAYSIWVLHLIPAWERVLDELLRVLRPGSPIVVTLGGKFGSGPWEEIASHFREIT
                     GAAPPGAGAMTEIDAAMDARGYRGTSLEPLMTTVEVSAEDILHRLEANQHSFTWVLDD
                     ATLSRG"
BASE COUNT          904 a         1608 c         1519 g          888 t
ORIGIN      
        1 cgatgaagga aggatggttg tgcgactcga tcttccacgc gacggcgacc ccgggggcca
       61 cctcgatgat ccctgcaccc tcgccgggac cgaccaggat gtgctcgcct tcagtgggaa
      121 gcgtcttcag atgcaccttg ctggacttgt aggaacagtg ctcgctccac atcaccgaga
      181 acatcgcgag ttctgtgtaa gtgggatcgc gatcgagtag ctcgcggatc ttgtcgaact
      241 cctcgtcggt caggccgagg tcccggtgga tgccggcctc cactctcacg tcccgagcat
      301 ctccggcacg cgtccggccg gatccgggga gcccgcctgc agcgcgtgat cgatcatcga
      361 ctcgaatatg acgcgaccgt cttcggaacc gaggagcgct tcacaggcac gttccgggtg
      421 aggcatcaac ccgaagacgg ttctgtcctt gttcgtgatg ccggcgacat cgtccagagc
      481 tccgttggga ttgtcgacgt agcggaggag gatgctgccg gaatcccgca ggtcggcaag
      541 cgtgtcgtcg tcgcagtacc agttgccttc gaagtgattg atcggaacgc gcaccacctg
      601 cccgacctcg gcacgcgatg tgaacggagt ggcgttgttc tcgacacgaa gatccgtcca
      661 ccggcacagg aacttgaggc cggcgttctt tcgcaacgct ccggggagca tccccgcctc
      721 gcacaggatc tggaacccgt tacagatccc gatcacgagc ccgccggtgg cggcgtgctc
      781 ttgcacggcg gccatcaccg gcgagaaccg agcgatggct ccggtgcgca ggtaatcgcc
      841 gtgagcgaag ccacccggga gaacgaccag gtccgtgtcg ggaagtgttt cggccgcatg
      901 ccacacgcgt tgggtctggc acccgagaga gcgaagagcg cgctcgacat cctgctcgca
      961 gttcgaaccg gggaacacca cgacggccgc tttcgcattc atcctgggtc tccgatcgtg
     1021 acctcgtaag actcgatcac cgggttcgcc aagaggcgct cgcacatctc gtcaacgcgt
     1081 cgacgcgcct ccgtttcgtc cgccgcgtca acgtggagct cgatgatctt cccgacgcgc
     1141 acaccggtca cgcctgtgta acccagagcc ggcagcgcgc gctcgatcgt ctgaccctga
     1201 ggatcggcga tcccgctctt tagcatcacg ttcacccgag ccgagtagga cacgccgcct
     1261 ccttggacac ggtgagggcc gtcggaacta cgagggccgc catggccacg gccaaacaca
     1321 gcgccgcgaa tgccgttgcg acgacggctc gacgagccgg tagacccccc acaacctcga
     1381 cacctctaac gaacagatag atcgaccaca ccgccaggct taccgacagc gcgatcgaga
     1441 aagcggccca ggccgtcgac aacgactcgt tcagaggttg agtcgtgaag gtcggtgtcc
     1501 ccacgatcag aagatccaac gggatcagga cgacgagcac caagatctga ggtagtgccg
     1561 aagcgcccca caccaaccgg agacccgagg agccggccga acctttcagg gactcgataa
     1621 cggatcgacc gaacgcgccc cacgcgaact gcgctatcaa cgccagcacg gctccgacca
     1681 cgagcgcgag agcgacgtag gccgggatgt actcggcggt acaaacctgc cgcagtttga
     1741 agagggctcc catcttcagc cacagggcgg cgagcgaagc cccgccgaga gctgtgagta
     1801 cgtaagtgga gagtccctcg atcggccagc gacccgaccg ttgtctgcgt tccgtcgcct
     1861 tcacggccgc ctcgaaccct tcgcgtggag cgagaaggac cgtgcggaac gtgagaccga
     1921 gtcgggcaga cgccctggag cggtccgcgc ggctcatcgg gcgcgcgggg tcggccacag
     1981 aatcgctcaa gcagatacct ctttgagcca cgcctcgaac ggccggccac tgacgagctc
     2041 gtaagcctcg acgtaccgat cgcgcgtcgc cgccacgact tcctcgggaa gatcgggggg
     2101 cggcggcgaa tggtcccaac ccgtggaatc gagccagtca cgtacgtact gcttgtcgaa
     2161 agagggctgc ccgcgaccgg gctcgtactt gtcctccggc cagaaccgac tggagtccgg
     2221 cgtcagcact tcgtcgatca ggatcacttc gccgtcgacc acaccaaact cgaatttggt
     2281 gtcggcgatg atgatccctc tctcgagcgc gagtgcggcg gccttcttgt agacctcgag
     2341 cgcatacgtg cgggctgtgt cgtatacgtc cttgccggcg atctcggccg cttcgccctc
     2401 ggtgatgttc tcgtcatgac cggtcgtagc cttggttgcc ggcgtcaaca gcacctccgg
     2461 caattggtcg gattccttca gaccctcggg cagctgatgg ccgcacacgg accgattggc
     2521 cttgtattcc ttccaccccg aaccggacag gtagcccctg acaacgaact cgatagcgac
     2581 gggctcggct cggcggcaca gcatcgcccg gccggcgaga tcgtcgatgc cgatgtcggg
     2641 aagatcctcg gcacggatcg agatcagatg gttcgggatc tcgtccatca cctcgaacca
     2701 gtaattggac agcccggtca gcaccttgcc cttgtccggg atgtcctggg tgaggacgac
     2761 gtcatatgcg ctgattcggt cggtggcgac gaggagcagg ctgtcttcgc cgatctcgaa
     2821 gagctccctg accttccccg agccgacgtg cttggcctgc gaactcacaa agcctccaat
     2881 cgccggaaga tctcgtctgc atactcaaga tagcgcgtgg ggtcgaagca tctttcgatt
     2941 tcgtcgggat cgagatgttg agtcacgtcg gcatccgact tgagcagctc tttcaggtgt
     3001 ctgccttcgt cccaggccgc catcgcattc cgctgcacca gttcgtacgc actctcgcgc
     3061 tgaagcccct tggccagcaa agcggtcaga accgactgtg aatggaccag cccccacgac
     3121 gcgtcgaggt tctcgagcat cctgtccggg tagaccctca gctcgtcgac gacccaacgc
     3181 agcttgtcca tcatgtagtc gagcaggatg catgcgtccc ccaaagtgat ccgctccacc
     3241 gatgaatgtg agatgtcccg ttcgtgccag agtgcgacgt tctcgaaacc cgttgctgcg
     3301 tacgcgcgca gaagccgtgc gagcccgcag atccgctcgc acgtgatggg gttcttcttg
     3361 tggggcatgg cggaagagcc tttctgcccc ttgccgaacg gctcctcgac ttcacgcacc
     3421 tcggtgcgct ggagatgcct gatctcttgg gcgaagcgtt cgagcgaagc ccccgtgatg
     3481 gcgatcgccg ccaagaactc agcgtggcgg tcgcgagcgg tgacctgagt ggcagcgtcc
     3541 tcgataccca gaccgagccg gtcacagacg aaagcctcga cgtcgggcgg gagctgtgag
     3601 tagctgccga ccgcgcccga gagcttccca acggccaccc gatcacgggc ggcacgcagc
     3661 cggtcccggt cgcgggagag ctcgaaagcc cacccggcca gcttcaggcc gaacgtcgtg
     3721 ggctccgcat ggaccccgtg tgtgcggccg gccatgaccg tgtccttgtg ttcgagggcc
     3781 ttgcggcgga tcgtctcggt cagtcgcccg actccctcta tcaagaggtc cccggcgtcc
     3841 cttagtgctg ctgccgtggc ggtgtcgaga acgtccgacg aggtcaatcc gtagtggatg
     3901 tggcgacccg cctcgcccac gttctcgcgc aggttctcga cgaacgcgac gacatcgtgc
     3961 tgccggacct cctcgatctc ggcgatccgc gcgacgtcga atgcggcctt ctccttgatc
     4021 tcggtcaagt cctcgacggg aacctgttcc agtcggaccc gtgcttcgac cgcgaggatc
     4081 tcgatctcca tccaccggga gaacttggct tgctcggaga agatcgccgc catctcctca
     4141 cggctgtaac gaggtatcac caaaccatcc taccgagcca ttcttggcta actgagtaac
     4201 gctccagcgc gctctgacga gacaagttcg cgttctatcg tggccggatg agctcgggcg
     4261 ggtcgctttc tttcgatcgg gcagccgagt actacgacgc gacgcgaggc cgttccgacg
     4321 agatcgagcg cagcgtcgct cgcctggccg agatccttgg gggccgcgac cggtgtctag
     4381 aggtcggcgt cggtacgggc cgggttgcgt tgcctcttca ccagaaaggg atcgatctag
     4441 tcgggatcga catctcggcg cgcatgatgg ccaagatgcg cgaaaaaaca tccggccgtc
     4501 acccattccc cctggtgcaa ggggacgcca cgcgcatacc gtttcgcgca cacagcttcg
     4561 acgctgctta ctccatctgg gtattgcacc tgatcccggc ctgggaacgc gtgttggacg
     4621 aattgctgcg tgttcttcgg cccggaagtc cgatcgtggt gacgctgggc ggcaagttcg
     4681 ggtcggggcc gtgggaagag atcgcatccc atttccgaga aataaccgga gcggcacctc
     4741 cgggcgccgg ggccatgact gagatcgatg ccgcgatgga cgctcgtgga taccgcggca
     4801 catccctgga gccgttgatg acgacggtcg aagtctccgc ggaggacatc cttcaccggc
     4861 tagaagctaa ccagcactcg ttcacgtggg ttctcgacga cgcgacgctc tcaaggggc
//
LOCUS       DATMED010000468         4803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1528689, whole genome shotgun sequence.
ACCESSION   DATMED010000468 DATMED010000000
VERSION     DATMED010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528689"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="VNP73_12210"
     CDS             complement(<1..1939)
                     /locus_tag="VNP73_12210"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HWL66731.1"
                     /translation="MDEELENRAEEVFGPNVWLVDEMYRRWQEDPRSVGAAWRDFFDG
                     YQPATGVVDPPEERPIEDEPAPRPVERDAAPARSTVPDEASPLRGVAARIAENMEASL
                     GVPTATSVRTMPAKLLEENRRVINRFLAGRRGGKVSFTHLIGYAIVKALEVRPAMKSS
                     FTEIDGVPHVVHRKEVNFGLAVDVEKADGSRILLVPNIKGAEQLDFAGFWSAYEELIR
                     KVKSNKLSPEDFAETTVTLTNPGTVGTSLSVPRLMPKQGLIVGTGAIGYPPEYEAADP
                     RSLAHIGVSKVLTITSTYDHRVIQGAESGQFLSYIHELLLGTDRFYDEIFTALKIPYE
                     PVVWSRDHAAIEGTDADLEKQARVGQLANMYRVRGHLLADLNPIAWEILSHRELDLSH
                     YGLTVWDLDREFLTDLPGPRKQTLRRILDTLRDAYCRSMGVEYMHISDPDQKQWIQYH
                     IERGVGEELSSEMKSHILDRLTAAEAFERFLHSKYTGHKRFSLEGADSLIPMLNELLE
                     DAAGSGMVEVVMGMAHRGRLNVLANVVGKPLREIFKEFEGDIDEATVQGSGDVKYHLG
                     MTGRFTARGGNELAVILASNPSHLEAVDPVVEGMTRSKQDLLGEDAEKRIMPVLLHGD
                     AAFAGQGVVAETLNMFDLPGYR"
     gene            complement(1975..2652)
                     /locus_tag="VNP73_12215"
     CDS             complement(1975..2652)
                     /locus_tag="VNP73_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWL66732.1"
                     /translation="MSDYVASYVGARERISELARGAGEDELQLKVPACPDWTVRELVS
                     HLVGVATDSVAGEIENLGNESWTTKHLEDRKGRSIDELIAEWEKAAVTLDAALADIHP
                     ALAGGLIGDVITHEHDLRGALDKPGARDMDLLELPIYTYVRWFGTRVKKAGLDPVEVE
                     SGDKSWIAGAGEAGPRVTADPFDLLRSLTGRRTRLEVKDLKWEGDPEPYLEVFSSYTY
                     ARSPLNE"
     gene            2738..3343
                     /locus_tag="VNP73_12220"
     CDS             2738..3343
                     /locus_tag="VNP73_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66733.1"
                     /translation="MFVQFIEGRCTDEAGVRKQWDRWQEELKPGAIGFLGSTAGITED
                     GTFFTAARFESKEAAQKNSDRPEQGQWWSETEQYLENVKFRDCTDIDEYAKGGSDDAG
                     FVQVMQGTVSDRERSRALDEKMMENMPNLRPDIIGGYQAWDADGKTFTAVNYFTTEAE
                     AREGEKKEVPAELQDAMKEWGEIMQGETRFIDLKEPWYSTK"
     gene            3340..3909
                     /locus_tag="VNP73_12225"
     CDS             3340..3909
                     /locus_tag="VNP73_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HWL66734.1"
                     /translation="MTDVKDLFRRSVEGFGERVHAVGDDQWNAPTPCTDWDVRTLVNH
                     IVYEEKWAPPLLEGKTIEEIGGDSFLGDLLGSDPKQAWKEASTNAVEAGLDPKVENTT
                     VHLSFGDFSGPDYLGQLITDHVIHAWDLARGTGGDDKLDPELVEYVYGLMQIQAEAWR
                     ASGAFANEVQVPNDADTQTKLLALTGRAS"
     gene            3925..>4803
                     /locus_tag="VNP73_12230"
     CDS             3925..>4803
                     /locus_tag="VNP73_12230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWL66735.1"
                     /translation="MKQNPRGGRARPSVFSRTFRLVMFSTFAYFTAVGVIIPVLPRFV
                     EGPLRGGSVAVGLAMGAFALTAVVLRPFVGRISDRRGRTILIIGGGLLMGASTAAYAL
                     ISDLEMLLVLRLVTGVGEAGFYVGVASIVNDLAPEERRGETLSYFSLALFGGLATGPV
                     LGEWLLVSSGFTATWLVAGASACVAGVVGLVLPDTRPPGTSEQASRLIHPAALKPGTI
                     LATHIWALATFTSFVPLYALSLGMGGSRYVFAMHSIIVLAIRSLGARLPDILGPRLSA
                     RLALICGSIGLPVMGFW"
BASE COUNT          910 a         1512 c         1464 g          917 t
ORIGIN      
        1 tcctgtatcc gggaaggtcg aacatgttca aggtctcggc aacaacgccc tggccggcga
       61 acgccgcgtc accgtggagg agtaccggca tgatccgctt ctccgcgtcc tcaccgagca
      121 gatcttgctt ggaacgggtc atcccctcga cgaccggatc cacggcttcc aggtgactcg
      181 ggttcgaggc caggatgacc gccaactcgt taccgccgcg cgcggtgaac ctgccggtca
      241 tccccaagtg gtacttgacg tcgcccgacc cctgcaccgt cgcttcgtcg atgtctcctt
      301 cgaactcctt gaagatctcg cgcagcggct taccgacgac gttagcgagg acgttcagcc
      361 ggcctcggtg ggccatgccc atgacgacct cgaccatgcc cgagcccgcg gcgtcctcca
      421 gcagctcgtt gagcatcggg atcaagctgt cggcaccctc gagtgagaac cgcttgtgtc
      481 ccgtgtactt ggaatggagg aatcgctcga aagcctcggc agcagtaagc cggtcgagga
      541 tgtgcgactt catctccgag ctgagttcct cgcccacgcc acgttcgatg tggtactgga
      601 tccattgctt ctgatccgga tcggagatgt gcatgtactc gacgcccatc gatcggcagt
      661 aggcgtcgcg caaggtgtcg aggatgcgtc tgagggtctg cttccggggg ccgggcaggt
      721 cggtgaggaa ttccctatcg agatcccaga cggtcagccc gtaatgcgag aggtcgagct
      781 ctcgatggct caggatctcc cacgcgatcg gattcagatc ggccaggagg tgacccctga
      841 cccgatacat gttcgccagc tgaccgaccc tcgcttgttt ctcgagatcg gcgtccgtgc
      901 cctcgatcgc ggcgtggtcg cgactccaca cgacgggctc gtaagggatc ttcagggcgg
      961 tgaatatctc gtcgtagaag cggtccgtcc ctagtagcag ctcgtggata tagctgagga
     1021 actgtccgga ctcggccccc tgtatgacgc ggtgatcgta ggtactcgtg atcgtcagga
     1081 ccttgctgac cccgatgtgg gcgagcgacc gcgggtcggc ggcttcgtat tcgggcgggt
     1141 agccgatcgc acccgttccg acgatcagac cctgcttcgg catcagcctc ggaacggaga
     1201 gactcgttcc gaccgtgccc gggttcgtga gagtgacggt cgtctcggcg aaatcctccg
     1261 gcgacaactt gttggacttg accttgcgta tcagttcctc gtacgcgctc cagaacccgg
     1321 cgaagtcgag ttgctcggca cctttgatgt tcggaaccaa caagatgcga ctgccatcgg
     1381 ctttctcgac atctacggcg agtccgaagt tcacctcttt gcgatgcacg acatgaggaa
     1441 cgccgtcgat ctctgtgaat gaggacttca tcgcgggacg tacttcgagc gccttgacga
     1501 tcgcgtaccc gatcagatga gtgaacgaga ctttgccgcc gcgccgtccg gcgaggaatc
     1561 gattgatcac gcgccggttc tcttcaagga gtttcgccgg catcgtgcga accgacgtgg
     1621 ccgtgggaac gccgagcgaa gcctccatgt tctccgcgat ccgggccgcg accccacgca
     1681 acggagaagc ctcgtccggc accgtcgatc tggccggcgc tgcatcccgc tctactggac
     1741 gcggtgcagg ttcgtcctcg atcgggcgtt cttccggagg gtcgacaacg ccggtagcgg
     1801 gctgatagcc gtcgaagaag tcacgccacg cggcaccgac ggaacggggg tcttcctgcc
     1861 agcgccggta catctcgtcc acgagccaga cgttcggccc gaagacctcc tcggcgcggt
     1921 tttcgagttc ctcgtccatc ccctttaagg gtagttcctg aggacgagtt ggaactactc
     1981 gttcaaggga gacctggcgt aggtgtagga ggagaagacc tcgaggtaag gctcgggatc
     2041 accctcccac ttcagatcct tcacctctaa acgggtgcga cgcccggtga gtgagcgcag
     2101 cagatcgaaa ggatccgcgg tgacgcgtgg cccggcctcc cccgctccgg cgatccagga
     2161 cttgtccccc gactcgacct ctaccgggtc cagtccggcc ttcttcaccc gggtcccgaa
     2221 ccagcggacg tacgtgtaga tcggaagctc gagaaggtcc atgtcccggg cccccggctt
     2281 gtccagcgcg ccgcgcagat cgtgttcgtg ggtaatcacg tcgccgatca atccgccggc
     2341 gagcgcggga tggatgtccg cgagcgccgc atccagagtc acggccgcct tctcccactc
     2401 tgcgatcagc tcgtcgatgg agcggccctt gcgatcttcg aggtgcttag tggtccagga
     2461 ttcgttgccg aggttctcga tctcaccagc aaccgaatcg gtagctaccc ccaccagatg
     2521 agagaccaat tcccggaccg tccaatcggg gcaagccgga actttcagtt gaagctcatc
     2581 ttcgcccgcc ccccgggcaa gctcggagat cctctctcga gcacccacgt acgaagcaac
     2641 gtaatcactc acccatagac cctaacgccc cccaacttga cgggagagat gacagcagta
     2701 atttcgaacg aaaggttcct cgagcaagga gggaacgatg ttcgttcagt tcatagaagg
     2761 tcgatgcacc gatgaggcag gcgttcgcaa acagtgggac cgatggcaag aagagctgaa
     2821 gcccggagcg atcggtttcc tcggctcgac cgcggggatc accgaggacg gcaccttttt
     2881 cactgcagct cgattcgaat ccaaagaagc tgcacagaag aacagtgatc gacccgaaca
     2941 aggtcagtgg tggtccgaaa ccgagcagta cctcgagaac gtgaagttcc gtgactgcac
     3001 cgacatcgac gagtatgcca agggtggctc cgacgacgcc ggcttcgtcc aggtcatgca
     3061 gggcaccgtc agtgatcgtg agcgctcgcg agccctcgac gaaaagatga tggaaaacat
     3121 gccgaacctt cgccccgaca tcatcggcgg ctaccaggcg tgggatgcgg acggaaagac
     3181 gttcacggcc gtgaactact tcaccaccga agcagaagca cgcgagggag agaagaaaga
     3241 ggtgccagcg gagctccaag atgcgatgaa ggaatggggc gaaatcatgc agggcgagac
     3301 ccgcttcatc gacctcaaag agccctggta ctcgacgaaa tgaccgacgt caaagacctg
     3361 ttcaggcgct cggtcgaagg cttcggggag cgcgtgcatg cggtaggcga cgatcaatgg
     3421 aacgcgccga cgccgtgcac ggactgggac gttaggaccc tcgtcaatca catcgtctac
     3481 gaggagaaat gggctccccc cctgctcgaa ggcaagacga tcgaggagat cggaggcgac
     3541 tccttcctcg gcgacctgct gggtagcgat cccaagcaag cgtggaagga agcatccacc
     3601 aacgcggttg aggccgggtt ggatccgaag gttgagaaca cgaccgtcca tctctcgttc
     3661 ggcgatttct ccggccccga ctatctcggc cagttgatca ccgaccacgt catccacgca
     3721 tgggatctag cccgaggcac gggtggcgac gacaagctag acccggagct cgtcgagtac
     3781 gtctacggct tgatgcagat ccaagcggaa gcgtggcgag cctccggagc tttcgctaac
     3841 gaggtccagg ttcccaacga cgcagacaca cagaccaaac ttttggcttt aacggggcgc
     3901 gcctcctagt agcgtcgcct ccatatgaag cagaaccctc gcgggggccg cgcgcgcccg
     3961 agtgtctttt cgcgcacgtt ccggctggtg atgttctcga cgttcgccta cttcaccgcg
     4021 gtgggagtca tcattccggt gctcccccgt ttcgtcgagg gacctctgcg tggaggaagc
     4081 gtcgcggtcg gactcgctat gggcgcgttc gccctcaccg cagtcgttct tcgacccttc
     4141 gtcggacgga tcagcgaccg ccgcggccga acgatcctga tcatcggcgg cggcttgctg
     4201 atgggagcga gcaccgccgc gtatgcactc atctccgatc tcgagatgtt gttggtccta
     4261 cggctcgtaa ctggtgtcgg ggaggccggg ttctatgtgg gtgtggcatc gatcgtgaac
     4321 gatctagccc ccgaagagcg ccgcggggaa acgctgagct acttctcact cgccctcttc
     4381 ggtgggctgg cgaccggacc ggttctgggt gaatggctac tcgtctcgtc gggctttacg
     4441 gctacatggc tggtagcggg agcaagtgca tgcgtggctg gagtcgtcgg actggtcctc
     4501 cccgacaccc gaccgcccgg cacgtccgag caggcgtcac gactcataca tcccgccgcg
     4561 ctcaaaccgg gcacgatcct ggcgacacac atctgggcac tcgccacgtt cacctctttc
     4621 gtgccccttt acgcactgtc gctcgggatg ggcgggtctc gatacgtctt cgcgatgcac
     4681 tccatcatcg ttcttgcgat ccgaagtctc ggcgccaggt tgccggacat cctcggcccc
     4741 cgcctatccg cccgcctcgc cctgatatgc ggttcgatcg gacttcccgt gatgggcttc
     4801 tgg
//
LOCUS       DATMED010000469        10062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13426
            k141_1149446, whole genome shotgun sequence.
ACCESSION   DATMED010000469 DATMED010000000
VERSION     DATMED010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942831
            Sequence Read Archive: SRR7976076
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:16:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,429
            Genes (coding)                    :: 2,415
            CDSs (with protein)               :: 2,415
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149446"
                     /isolate="SMAG_U13426"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.9813 N 109.5168 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VNP73_12235"
     CDS             complement(<1..198)
                     /locus_tag="VNP73_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66736.1"
                     /translation="MRRILVLGLVVGAVALALPLNAARAQTECEWLAGDFHVHTVYSH
                     DSWGGPDDPGTPPDEAHTFGWT"
     gene            complement(203..2116)
                     /locus_tag="VNP73_12240"
     CDS             complement(203..2116)
                     /locus_tag="VNP73_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66737.1"
                     /translation="MSWNWNDAPGSSPSHRRKATLRKLFTSLLVGMLSVGVAWAGTGL
                     VPINDTGVTSSDTDDGNWLQSLAGDGDSDGGSASAAAAGTDEPLQANQVYAGAHAVNI
                     APNPAKYNGEWEKDQDHCQTNAAPIGFGNPQETEPEHLADTRVRWNENINCIYTGGFD
                     IGPAHAVSSFDEEFGLYARATAISDGNDTVVLMILDGAYYFAEYANMCNTVVDHASDF
                     DGEGPGANQYTQDCGFRQMAESMAAKYGFTGADARGEHGIEPSSFFLASSHAHASPDF
                     VGAWGGVPRWYMQQVEDAIRQAVYTAIDTMQPTYVEASEILYRAGNNSRRRHYHAAEE
                     AGYAWMRFVAEEDAEPVCTTPPPSPAPSPTKPKGKPSPSPTATPTPTPSCTQPPPNKR
                     AVATIGTYAAHPTDGSGGRAYGDLSAVYAKRAEARFGGTGLYFQTGFGNMTSGPGRVQ
                     LAGNLVDMLPAVGAGPQVTGNLDVRTALRTFNHPVTNSALGGGGITGIFDRPMGEGPA
                     AVSISRDSLGVDAGHMPYKRCNSASPVSVNTSVSVARVGNLLVTGGPGELFSNYTNTI
                     KEKHAGGLAFPLSLVNDGLGYIMQSVETDHVGRQAVGFVNGPLSEYEDAYSIDACFGD
                     HALEQTLAGMDGL"
     gene            complement(2262..4175)
                     /locus_tag="VNP73_12245"
     CDS             complement(2262..4175)
                     /locus_tag="VNP73_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66738.1"
                     /translation="MTWNWDAPPGASRNRQIVRKLFVSLLVGILSIGVAWAGSGLVPV
                     NAVRQNEQSAAANNNFVGIEEQDSGEQEIAAAALEQLEEGQIYAGAAKTSLFPRPEDY
                     AEQFPGAIWEQDEAQCEVMDENAPNGFAFLDDTRVRWHENPNCLYMGGYGLGPTNAIT
                     SWDNDYGLWVRSIAMQDAQGDTVVLTLIDAVYWEAHYDSMCAGEPATPAGRGDQDCGL
                     IEIAERLGDETGLPAESFIFASTHSHTAMDFIGGWGGVPEWYMQQAEDSLMQTARDAL
                     ATLEPAVLEAGESIVRERNGERRDFYHSAEDDNLSWFRLIDADSESEPVCTTPAPAPT
                     PTLPPGQAKKQTPSPQPTPTPTCTGGSPARAIATVGAYAAHPVTEDEGAGEGDADFPA
                     VFAKAVEDKFGTAAEPGVGMFLQTGLGNMSPRGNKVDMGEGLASYIPALKQGRQVTNP
                     DVRVGRTFWDQPATNIPLGHLGAAGIFDRTFNRQPAVVTAGDGSSQHAAVGDGNPENK
                     KCRSASPISVNTSVSAAKIGSLWITGGPGEVFSNLTNTIEERNPGGVTLALGLVNDGL
                     GYIVESFETDHVGRQGVGFVGDANAHDDGNPVQDAIPNEPLALSEYEDAYSIDHCFGD
                     ATLQHTLELLGTL"
     gene            complement(4252..4653)
                     /locus_tag="VNP73_12250"
     CDS             complement(4252..4653)
                     /locus_tag="VNP73_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66739.1"
                     /translation="MKTRTRFIGTCMVVVALLAAFAPPATTAPAPIQVDVRQTDLGLE
                     IVWTCAAVAVGAATETTISTCRVEDRAFGFDTEAPSVTQDGPVAATWTEETGLQGGED
                     HWRLDTWVCWEASATFVGGGTGSSSGCQLVS"
     gene            complement(4804..4980)
                     /locus_tag="VNP73_12255"
     CDS             complement(4804..4980)
                     /locus_tag="VNP73_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66740.1"
                     /translation="MALSLSELVEAGLVTDMKDPHLSEKGREWLRQLESVDHHDMDME
                     DAADLVLSTNGLFR"
     gene            5174..6217
                     /locus_tag="VNP73_12260"
     CDS             5174..6217
                     /locus_tag="VNP73_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66741.1"
                     /translation="MRRPFVTFLIATLIIASAAVPGSAQEAVFTGNEILFTGWRDRAA
                     VYSVDPASGEQRRLTSRRAAVPVWSPARDRIAFIRYWSTSDWIPDVKSSLMVLDRDGT
                     IHQLTDGKTNDFDAAWSPDGSRLVFSRYTTEDNIISVHLFVINADGSGLTELTSGKGI
                     TDAEASWSPDGSKIAFTHNSTFQSILIIDVESGETTALGGDPDILDSQPRWSPDGRYI
                     AFNRYFFSNDQSDIYVADLEAKRFIALTKSDKIEEGPVWSPDGTEIAFVKVLWRDFNP
                     EIFVKSLGGGAATRLTNQKKGDHYPVWSGDGSQIAFVRGDGRDQDVYLVSRDGGRGRR
                     LTRTPIQESTLDW"
     gene            6289..6855
                     /locus_tag="VNP73_12265"
     CDS             6289..6855
                     /locus_tag="VNP73_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWL66742.1"
                     /translation="MQAEESNRGLSPIRAFLILLGVLAAVTAVVLLTRPTESVPAPGS
                     PAGAVDHSLTNAEALARLSDLQRLERQAFAETDASLLSQVYTSTSPLLKDARNGLKKL
                     QKDNVAAVFERKQEELVVLVNESSVVRVRETSIFDIRFVSRDGSPVDQRGSADLQTTE
                     WVLRFAGGQWLIDDAILRSSKPVSKDGQ"
     gene            7071..7655
                     /locus_tag="VNP73_12270"
     CDS             7071..7655
                     /locus_tag="VNP73_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWL66743.1"
                     /translation="MIANRCTGIASGDSFSAFSNSATCSSFVAPTPPRSAPGNVVATA
                     RRPSPQQIALSLVQREIAFAPRPRLRISPSQVGLTGLDSFFWLADRPQPISASAGPVT
                     AQAYPAQYTWDFGDGERRTTSTSGRRWTRRRDGNVAHLYEKRGAYSVSVDVIWEARWR
                     VGTGAWQPLGYFTTSDSAPYSVRQVVALLVRSRR"
     gene            7989..8693
                     /locus_tag="VNP73_12275"
     CDS             7989..8693
                     /locus_tag="VNP73_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWL66744.1"
                     /translation="MFSHRTAGTLMELDGVPEGFVEVVTASPSRLSNVIVHRLRPTDQ
                     PRTVRFQGFRTTSVERTLLDLFAVIDPESAELALEDALRRRLTTLDRLWTLSTGFGCG
                     RNGLRAFRRCLLLHDHRDGSLQSRMEAGLRRIVNTLPDPRAEPQYRVTVDDHRYRIDF
                     AYPHIKLGIEAHGLKWHMGRERWIKDLRRDRRMKTIGWTLLYFPWDDIQLEPRRVATE
                     ILEIRTSLERALLNET"
     gene            complement(8721..9146)
                     /locus_tag="VNP73_12280"
     CDS             complement(8721..9146)
                     /locus_tag="VNP73_12280"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWL66745.1"
                     /translation="MSDQQIDAQQLADLVKGMSDDELKDSIKAMGIDETMKNIFQGMQ
                     DAFKPEKAAGVSSVLQYDIETEEGTKTWKVEIADGACKTSEGAATDPRLTLKLALVDF
                     VRLIMGQADGTQLFMTGKLKLTGDMMFAMQMQGFFEQPS"
     gene            9248..9838
                     /locus_tag="VNP73_12285"
     CDS             9248..9838
                     /locus_tag="VNP73_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWL66746.1"
                     /translation="MLRPRLVRLAAPLVLAAATACNMHQVDLTVQAPITMQEEPTGYE
                     AALVTRVIDGDTIEVEIQEVVPGPGAGNTVPDNGYDVRLTGIDTPESVKPGSPVECFG
                     KEASAALKALLDGHEVRLVKDVEDTDRYDRLLRYVYMGEEMANARMVANGYASAYTYP
                     PNVRHSELFVRLQAEARDNNRGLWHPDACNGDTETQ"
     gene            9835..>10062
                     /locus_tag="VNP73_12290"
     CDS             9835..>10062
                     /locus_tag="VNP73_12290"
                     /inference="COORDINATES: protein motif:HMM:NF042842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HWL66747.1"
                     /translation="MTNVLVRVFAFSLLPILLAALHVRLSSDSKDRLQKVEVYLIYLF
                     FGVGASGLSGFFGHVFTPDAVAESIGWAKGSP"
BASE COUNT         1775 a         3137 c         3180 g         1970 t
ORIGIN      
        1 cgtccaaccg aaggtgtggg cctcgtcagg gggcgtgccg ggatcgtccg gtccacccca
       61 ggagtcgtgc gagtaaacgg tgtggacgtg gaagtccccg gccagccact cgcactcggt
      121 ctgtgctcgt gccgcgttaa gcggcagtgc cagcgcgacc gcgccgacta cgaggccgag
      181 aacaaggatc cgccgcatat gtctagagcc cgtccatgcc ggcgagcgtc tgctcgagag
      241 cgtggtcccc gaagcaggcg tcgatggagt aggcgtcctc gtactcggac agtggtccgt
      301 tcacgaagcc gaccgcttgt cgtccgacat ggtcggtctc gacgctctgc atgatgtagc
      361 cgagcccgtc gttcacgagt gacagtggga acgcgagccc accggcgtgc ttctctttga
      421 tcgtgttcgt gtagttggag aacagctctc ccggcccccc cgtgacgagg aggttcccca
      481 cacgtgcgac cgacacggac gtattcactg aaaccggcga ggccgagttg cagcgcttgt
      541 agggcatgtg ccctgcatcg acgccgagac tgtccctact gatactcacg gccgcggggc
      601 cctcacccat tggacggtcg aagatccccg tgatgccacc accaccgagc gcactgttcg
      661 tcaccgggtg gttgaaggtc cttagagccg ttctgacgtc gaggttgccg gtgacttgag
      721 gtcccgcacc aacggcaggg agcatgtcca cgaggttccc agccagttga acgcgtccgg
      781 ggccactcgt catgttcccg aagccggtct ggaagtagag gccggtccca ccgaagcgag
      841 cctcggccct cttcgcatac accgcggaga ggtctccgta ggcgcgaccg ccgcttccgt
      901 ccgtcgggtg agctgcatag gtgccgatcg ttgcaaccgc acgcttgttg ggcggaggtt
      961 gcgtgcagct cggagtagga gtcggggtag ccgtcggtga aggagagggc ttgcccttcg
     1021 gcttggtcgg gctgggcgcg gggctcggcg gcggtgtggt gcacacaggt tccgcgtcct
     1081 cctcggcaac gaaacgcatc cacgcataac cggcttcctc cgcggcgtgg tagtgccgac
     1141 gccggctgtt gttgccggcg cggtagagga tctcagatgc ctccacatat gtgggctgca
     1201 tcgtgtcgat agcggtatag acggcctggc ggatcgcatc ctcgacctgc tgcatgtacc
     1261 agcgcgggac accaccccat gcccccacga agtcggggga cgcatgtgcg tggctcgatg
     1321 cgaggaagaa gctcgacggc tcgatgccgt gctcacctcg agcgtcggca ccggtgaagc
     1381 cgtacttcgc ggccatcgac tccgccatct ggcggaagcc gcagtcctgc gtgtactggt
     1441 tggcgccggg gccctcaccg tcgaagtcgg acgcgtggtc tacgaccgtg ttgcacatgt
     1501 tggcgtactc ggcgaagtag taggcgccgt cgaggatcat cagaacgacg gtgtcgttcc
     1561 cgtcgctgat cgcggtcgcg cgggcgtaca gcccaaactc ctcgtcgaag ctgctgaccg
     1621 cgtgtgcggg accgatgtcg aagccaccgg tgtagatgca gttgatgttc tcgttccagc
     1681 gaacgcgcgt gtcggccaag tgctcgggct cggtctcttg gggatttccg aatccgatgg
     1741 gggccgcgtt cgtctggcag tggtcctgat ccttctccca ctctccgttg tacttggcgg
     1801 ggttgggggc gatattgacc gcgtgcgctc cggcgtagac ctggttcgcc tggagcggct
     1861 cgtccgtgcc cgccgcggcg gcggatgccg aaccgccgtc ggagtctccg tccccggcca
     1921 ggctctgcag ccagttgccg tcgtcggtat ccgagctcgt cacgccggtg tcattgatcg
     1981 gaactaatcc cgtgcccgcc cacgcgacgc cgacggaaag catgccgacg agcagtgacg
     2041 tgaacagttt cctgagggtc gccttgcggc gatgcgaagg tgacgatccg ggggcgtcgt
     2101 tccagttcca actcatgtgg agatctcctt ggacgaagat gggggttgcc gggaaagttc
     2161 gcttctacgt acgaaagtcc tgcgggcgcg cacctgacgc gcgcatgaaa aaagccccgg
     2221 gacatcgccc ggggcttctt tgtccgttat gtctgattcg cttacagggt gccgagcagt
     2281 tcgagagtgt gctgcaacgt cgcgtccccg aagcagtggt cgatcgagta agcgtcctcg
     2341 tactcggaga gtgcgagcgg ttcgttgggg atggcgtcct gaaccgggtt gccgtcgtcg
     2401 tgagcgttgg catcaccgac gaagccgacg ccctggcgcc cgacgtggtc ggtctcgaag
     2461 ctctcgacga tgtagccgag gccgtcgttc acgagaccaa gagccagcgt gactccaccc
     2521 gggttccgct cctcgatggt gttcgtcagg ttcgagaaca cctctccggg cccaccggtg
     2581 atccacagcg atccgatctt ggctgcacta acagatgtgt tgacggagat aggcgatgcc
     2641 gagcggcact tcttgttctc ggggttgcca tcgccgaccg ctgcgtgctg cgagcttcca
     2701 tcgccggccg taacgaccgc gggctgccta ttgaaggtgc ggtcgaagat cccggcggca
     2761 ccaaggtgcc cgagagggat gttcgtcgcc ggctggtccc agaaagtacg tccgaccctg
     2821 acgtccgggt tcgtcacctg acgaccttgc ttcagagcgg ggatgtacga ggcgagtccc
     2881 tcacccatgt cgaccttatt gccgcgcggc gacatgttgc cgagaccggt ttgcaggaac
     2941 atgcccacac caggttccgc ggcggttccg aacttgtcct cgactgcctt agcaaaaacg
     3001 gcggggaagt cggcgtcacc ttcgcccgcg ccctcgtcct cggtgacggg gtgggccgcg
     3061 taagcgccga cggtcgcgat cgcgcgtgca ggcgatccgc cggtgcaggt cggcgtcggg
     3121 gtgggctggg gagaaggggt ctgcttcttg gcctggcccg gcggcaacgt cggggtcggt
     3181 gcgggagccg gagtcgtgca caccggttcc gactcgctgt cggcgtcgat caaacggaac
     3241 cacgacaggt tgtcatcctc ggcggagtga tagaagtcgc gccgctcgcc gttgcgctcc
     3301 cggacgatcg actcgcccgc ctcgaggacg gcgggctcca gcgttgccag tgcatcgcga
     3361 gcggtctgca tgagcgagtc ctctgcctgc tgcatgtacc actcggggac gccaccccaa
     3421 ccgccgatga agtccatcgc ggtgtggctg tgcgtggaag cgaagatgaa gctctccgcc
     3481 ggaagtcccg tctcgtcgcc gagacgttcg gcgatctcga tgaggccaca gtcctgatca
     3541 ccacggcccg ccggagtggc aggctcgccg gcgcacatcg agtcgtagtg cgcttcccag
     3601 tagacggcgt cgatcagggt caggacgacg gtgtcgcctt gtgcgtcctg catcgcgatc
     3661 gagcgcaccc acaatccgta atcgttgtcc cacgaggtga tcgcgttcgt ggggccgagc
     3721 ccgtagccgc ccatgtagag gcagttgggg ttctcgtgcc agcggacccg ggtgtcgtcg
     3781 aggaacgcga acccgttcgg tgcgttctcg tccatcacct cacactgggc ctcgtcctgc
     3841 tcccatattg cgcctgggaa ctgttcggcg tagtcctcgg ggcgggggaa cagtgaggtc
     3901 ttagccgcgc cggcgtagat ctggccctct tcgagctgct cgagtgcagc ggcagcgatc
     3961 tcttgctcgc cggagtcctg ctcctcgatg ccgacgaagt tgttgtttgc ggccgcgctc
     4021 tgctcgttct ggcggacggc gttcaccggc acgaggccgg agcccgccca cgcgaccccg
     4081 atggacaaga tgcccacgag gagcgatacg aatagcttgc gaacgatctg ccgattgcga
     4141 ctcgcgccgg gaggcgcgtc ccagttccag gtcatggttg ttgtctcccg ctgtatgagg
     4201 ttgcagctct gtgaggggaa atttcgcccc gggccggtta tttcctgcgg atcagctgac
     4261 tagctggcag ccggacgacg aaccggtgcc gcctccgacg aaggttgcag atgcttccca
     4321 gcaaacccag gtgtctaggc gccagtggtc ctcgccgccc tggaggccgg tttcttcggt
     4381 ccaggtcgcg gccacggggc cgtcctgagt cacagaggga gcttcggtgt cgaacccgaa
     4441 cgcgcggtcc tcgacacgac aggtcgagat ggtcgtttcg gtcgcggctc cgacggcaac
     4501 ggcggcgcag gtccacacga tctcgagacc gaggtcggtc tgtcgcacgt caacctgaat
     4561 cggagcgggg gccgtcgtcg cggggggcgc gaacgccgcc agcagggcca ctaccaccat
     4621 gcacgtaccg atgaatcggg tgcgggtttt catcttgttc cgaacctccc tttggtcgct
     4681 ctgctgcgac cgacggtcaa acggttcgcc gggaggcggg gcactccttc ttatgaggag
     4741 ggaagttgga cgcgaaagag ccccggcaga tgccggggcc cgtttatcga gggcttgatc
     4801 gtgttaccgg aacagcccgt tcgtcgagag aacgagatcg gccgcatcct ccatgtccat
     4861 atcgtggtga tcgacgctct ccaactggcg cagccactcg cggcccttct cgctcaggtg
     4921 agggtccttc atgtccgtca ccagaccggc ttccacgagc tcgctaagac tcagtgccat
     4981 tagtcctcct tcaggtcaga cctgcgtatt ggtttcttgg actctcaagg tagcgaacgg
     5041 ttacttgacc gtcaggtcta cctaggcggt ggcgcggaac gttcatcgaa ctttcacaaa
     5101 atgcgtagga acacattgct cgaatggggc gtcggataga agatcgagac agcactcatt
     5161 caagggggga ccgatgaggc ggccgttcgt gactttcctg atcgcaacgc taattatcgc
     5221 gagcgcggcg gtcccgggtt cggcgcaaga agcggtcttc actgggaacg agatcctctt
     5281 caccggctgg cgtgaccgag cggccgtcta ttcggtcgac ccagcatcgg gggagcagcg
     5341 tcgcctcacc tcgcgcaggg cggccgtgcc ggtgtggtcc ccggccagag atcggatcgc
     5401 cttcatccgc tactggagca ccagcgactg gatcccggac gtcaagtcgt ctctcatggt
     5461 cttggaccgg gacgggacga tccaccagct caccgacggc aagacgaacg atttcgacgc
     5521 agcctggtcc ccggatggga gccggctcgt tttctctcgc tatacgaccg aagacaacat
     5581 catctcggtc catctattcg tgatcaatgc agacggctcc ggcttgactg aattgaccag
     5641 cgggaagggg atcaccgacg ccgaggcgtc gtggtcaccg gacggctcaa agatcgcttt
     5701 cactcacaac tccaccttcc agtcgatctt gatcattgat gtcgagtcgg gagagaccac
     5761 agctctgggc ggagatccag acatccttga ctcgcaacca cgttggtcac ccgatgggcg
     5821 ctacatcgcg ttcaaccggt atttcttcag caatgatcag agcgatatat acgtggctga
     5881 tctcgaagcc aaacgattca ttgctcttac caagagcgac aagatcgaag aggggccggt
     5941 gtggtccccg gatgggacag agatcgcgtt cgtcaaggtg ctgtggcgag acttcaatcc
     6001 cgagatcttc gtgaagtcgt tgggtggcgg ggccgccacg aggttgacca accagaagaa
     6061 aggtgaccac tacccggtgt ggagtggtga cgggagtcag atcgctttcg tcagaggcga
     6121 tggccgggac caagacgttt acctggtgag ccgggatggg ggccggggcc gaaggctgac
     6181 gaggacgcct atccaggagt cgacgctcga ctggtgagcc tgcccggcgc cgttttgaca
     6241 aaccatggaa aatcggagcc agcggaagcc tctaactaga ggtggcgaat gcaggcagag
     6301 gaatcgaatc gcgggctgag cccgatccgg gccttcctca tcctgctcgg agttctggca
     6361 gcggtgaccg cggtagtact cctaacgcgc ccaacagagt cggtgccagc tcccggctcg
     6421 ccggcaggag ccgttgacca ctccctaacc aacgccgaag ccctagcccg cttgagcgac
     6481 cttcagcggt tggaacgaca ggcgttcgct gaaactgacg cctcgcttct gtcgcaggta
     6541 tacacctcga caagccccct gcttaaggac gcacggaatg gtttgaagaa actgcagaaa
     6601 gacaacgttg cagcagtctt tgaacgaaaa caagaggaac tggttgtctt agtgaacgaa
     6661 tcctcggtgg tacgcgttcg cgagacaagc atctttgata ttcgtttcgt ctctcgtgat
     6721 ggaagccctg tggaccagcg ggggagtgcg gaccttcaaa caacggaatg ggttcttagg
     6781 ttcgcgggtg ggcaatggct gatcgacgac gcgattctta ggtcgagcaa gccggtctct
     6841 aaggacgggc agtgagaaga ggactcgttc ttgcagccgt tgcagcgctc ctgggtccca
     6901 gcccgtcttt tgctgctgat ggcgaggccc gcgtttttga acctccacgg tcagaagtgg
     6961 ctggcgttga ggcaacggcc cagaagtatt tcccgcctga ccccggtaat tcagagccga
     7021 cgtccgaggc ggatgtagtt cgttctataa gttcccaagg acccgttgtg ttgattgcta
     7081 atcggtgtac tgggattgct tcgggggatt cgttctcggc gttctctaac tcggctactt
     7141 gctcgtcgtt cgtcgctccc acgccgccgc gatctgcgcc gggcaacgtc gtggctacgg
     7201 cccgacggcc ctcgcctcaa cagatcgctc tctccctcgt gcaacgcgag atcgcgttcg
     7261 cgcctcgacc tcggctccgc atatcgccgt cgcaggttgg gctcacgggg ctcgactctt
     7321 tcttctggct cgccgatcgt cctcagccga tctcggcttc cgccggcccg gtaaccgcgc
     7381 aggcataccc ggcccaatac acgtgggact tcggtgacgg tgaacgtaga accaccagca
     7441 cctccgggcg ccgctggaca cgtcgccgcg acggcaacgt cgcccacctc tacgagaagc
     7501 gcggcgccta cagcgtctca gtcgacgtca tctgggaagc acggtggcgc gtcggcacgg
     7561 gcgcgtggca accgctcggc tacttcacga cctcagactc ggccccctac tcggtccgcc
     7621 aagtagtagc gctcctagtt cgctcccgcc gctagcgcca ctctgatcag caaaacccag
     7681 caaaacgtgg gctttcgctg atcagagtgc ggtgagccac agcgctcccg tcgcaaagcc
     7741 agttgcctga tgctgtgccc aggtacgaga gtgcgatatc gaggtgtgag agacgtagtt
     7801 cactcaatca cggtgtgctt tctcgggagg aagcactggc cgaaggtctt acggcgaatg
     7861 tgatcgaacg actcgtgcgc acatgtcgtt ggttccgcct tcagcctcgt acttactgtc
     7921 cggccggagt cccaatcacc tggcacacga gacttgcggc cgtccaggca tccctcggat
     7981 cgaaatgtgt gttctcccat cggactgcgg gcacattgat ggagctggac ggcgtcccgg
     8041 agggattcgt ggaggtagtc acggcaagtc ctagccgtct ctcgaatgtg atcgttcatc
     8101 gattgcgtcc cactgatcag ccgcgaaccg ttcgcttcca gggcttccgg acgacttccg
     8161 tcgaacgaac attgttggat ctattcgcgg tgatcgatcc tgagagtgcg gaactcgctc
     8221 ttgaggatgc cctgcgtcgc aggctgacga cactcgatcg cctgtggaca ctatcgacgg
     8281 gcttcggttg cggacgaaac ggccttcgtg ccttccgccg atgcttacta cttcatgacc
     8341 atcgggatgg ctcgctgcag agtcggatgg aagccggctt gcgaaggatc gtcaacaccc
     8401 ttcccgatcc ccgagcggaa ccgcagtacc gcgtgaccgt agatgaccat cgttacagga
     8461 tcgactttgc ctatccccac atcaagctgg ggatcgaggc gcacggattg aagtggcaca
     8521 tgggccgcga gcgatggatc aaggaccttc gccgagatcg ccggatgaag acgatcggtt
     8581 ggacgctgct ttatttccct tgggacgaca tccaactcga gccgagaagg gtagccaccg
     8641 agatcctcga gatccgtacc agtctcgaac gcgctctgct caacgaaacc tagcgaaggc
     8701 cagggttttg ctgagcagag ttagctcggt tgttcgaaga agccttgcat ctgcatcgcg
     8761 aacatcatgt cgccggtcag cttcagcttg ccggtcatga acagctgcgt cccatcggcc
     8821 tgacccatga tcagtcgcac gaagtccacc agcgccagct tcagcgtgag ccgaggatcc
     8881 gtcgcggccc cctcggaggt cttgcacgcc ccgtccgcga tctcgacctt ccaggtcttc
     8941 gtgccctctt ccgtctcgat gtcgtactga aggaccgagc tcacgcccgc cgccttttcg
     9001 ggcttgaacg catcctgcat gccctggaag atgttcttca tcgtctcgtc gatgcccatg
     9061 gccttgatgg agtccttgag ctcgtcgtcg gacatcccct tgaccaaatc ggctagttgc
     9121 tgcgcatcga tctgctgatc ggacactgct cacctccagg aaagcctgat tgaccgggca
     9181 aatctagacg cccacgctgt gccacgcgtc acgcaggcgc cgtcgggcga ccccgtaacg
     9241 tgcccggatg ctcaggccgc gcctggtccg cctcgccgcg cccctcgtcc tggcggccgc
     9301 gaccgcctgc aacatgcatc aggtcgacct caccgtccag gcccccatca cgatgcagga
     9361 ggagccgacc ggctacgagg ctgcactcgt cactcgggtt atagacgggg acacgatcga
     9421 ggtggagatc caagaggtcg tccccggccc cggcgccgga aacaccgttc ccgacaacgg
     9481 atacgacgtg aggctcaccg gcatcgacac gcccgagtcg gtcaagccgg gctccccggt
     9541 ggagtgtttc ggcaaggaag cctcggccgc gctgaaggcg ctgctcgacg gccacgaagt
     9601 gcgcctcgtc aaggacgtcg aggacaccga tcgctacgac cggctccttc gctacgtcta
     9661 tatgggagag gagatggcga acgcacgcat ggtcgcgaac ggctacgcct ccgcttacac
     9721 gtacccgccc aacgtccgtc attcggagtt gttcgtccga ctccaagccg aggcgagaga
     9781 caacaaccgc ggcctttggc accccgatgc atgcaacgga gatactgaga cccaatgacg
     9841 aatgtcctcg tccgcgtgtt cgcgttctca ctactaccga tcttgcttgc cgcccttcat
     9901 gtccgtcttt ccagcgactc gaaggatcgc ctgcaaaagg tagaggtgta cctgatctac
     9961 ctattcttcg gcgtgggggc aagcggcctc agcggtttct tcggtcacgt cttcactccc
    10021 gatgccgtgg cggaatcgat cggctgggcg aagggcagcc cg
//
